LOCUS       DAOPHX010000001        74820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_294_length_74820_cov_7.074126, whole genome shotgun sequence.
ACCESSION   DAOPHX010000001 DAOPHX010000000
VERSION     DAOPHX010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 74820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 74820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74820
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_294_length_74820_cov_7.074126"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..92
                     /locus_tag="PK502_00005"
     tRNA            4..92
                     /locus_tag="PK502_00005"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38..40,aa:Ser,seq:gct)
     gene            99..175
                     /locus_tag="PK502_00010"
     tRNA            99..175
                     /locus_tag="PK502_00010"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:133..135,aa:Pro,seq:tgg)
     gene            434..1531
                     /gene="meaB"
                     /locus_tag="PK502_00015"
     CDS             434..1531
                     /gene="meaB"
                     /locus_tag="PK502_00015"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPG99231.1"
                     /translation="MKQRKNKDDESALFVAPGIDQPPVVNEQAVQKFRQQKRRQPSID
                     EFVGGILARNNTILSQAITLVESSLPRHYELAQQLIERCLPYSGNSIRVGITGIPGVG
                     KSTFIESLGTMLTSQGHRLAVLAIDPSSTRSKGSILGDKTRMESLSADANAFIRPSPS
                     AGSLGGVARKTRETIILCEAAGFDTVFIETVGVGQSEVSVHSMVDFFLLLMLAGAGDE
                     LQGIKRGIMEMADLIAITKADGANISRSKLAQAEFQNALHLFPMPESGWVPKVVTCSA
                     LNKVGIDEIWREMVAYADFTRKNGYFVSRRNRQAKYWMHETINEALKNKFYADRLIHE
                     QVRELEKEVVANRLSPFAAAQLLLDRYFSLK"
     gene            1670..2197
                     /locus_tag="PK502_00020"
     CDS             1670..2197
                     /locus_tag="PK502_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018451909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPG99232.1"
                     /translation="MDKKQFLSTILNRKSVRSFTGKPLGQDDMELLLRAGMAAPSAHN
                     LQPWKFIAIVDRQILNSLAEKLPYAKMLFEAPAAIVVCGDLTKTGSSPKDYWVQDCSA
                     ASENILLAAEALGLGAVWTGVYPRAERVETVQGILNLPSEIVPLNVIPVGTPKGEHQA
                     KDKFKSENIQWNRWP"
     gene            complement(2194..3570)
                     /locus_tag="PK502_00025"
     CDS             complement(2194..3570)
                     /locus_tag="PK502_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPG99233.1"
                     /translation="MVKTAKPKKEKKESRIQRLKKKFEQIYSFITHDIWVLQVDELSP
                     KLKAFYKYLRVGLLALRGFNEKKVSVKASALTYYTLMSIVPIFAMVFGIAKGFGLDKF
                     LEQQIRSSLEGQEEVMNKLITFSSSLLERTGGGIIAGIGVVLLFWSVVMVLSSIENAF
                     NDIWQIEKPRTYVRKFTDYLSIMVIAPILLLAASGIQIYLSTQVSTIAEKSQIIHIIS
                     PVLLSGFKLVPYLLIWILFSFLFITLPNTKVSIVSGIIAGILSGTAFLIVQWAYVYLQ
                     IGVSKYNAIYGSFAALPLFLVWVHTSWLIVLFGAEVSFAYQNVEMYEFEKETDQISHF
                     NRKVLSILILNTIIKRFNNGEEPLTSLDLSKQLKIPQRLMRNLLTQMVECGFLNEVVT
                     PIAKNYAYQPARHIDHLTLAYIEDKLDNHGLKIEPNSDDYKHIAEVYRNFNEKYKALS
                     KETLIGSL"
     gene            complement(3685..4809)
                     /gene="dprA"
                     /locus_tag="PK502_00030"
     CDS             complement(3685..4809)
                     /gene="dprA"
                     /locus_tag="PK502_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPG99234.1"
                     /translation="MSADPELVYKIGIELIPKVGSISAKKLIAYCGSAEAVFKQKKGS
                     LLKIPGIGEATAAEIVAQTVLSRAENELLFIEKFGIKVLYFTDAAYPYRLKQCEDGPV
                     VLYVKSNINIDFNTDKVLSIVGTRRATEYGKAMCEDLVSGLAEKGYRPIIISGLAYGI
                     DVTAHRAALKRNLPTVAVLGHGLNTIYPQSHRSVAKAILNQGALVSDFTSDAVFDRNN
                     FLRRNRIIAGMADATLVVESALEGGALVTADIANSYNRDVFAVPGNVNSVYSQGCNQL
                     IKQNKAALVESADDLEFLMGWTTSKVNSSRQYSINFDKFSADEQKILNFLKEKGEETV
                     DVIAMDTGLQVSKVLSLLLNLEFAGVVRSMPGKVFALAIG"
     gene            complement(4806..5288)
                     /locus_tag="PK502_00035"
     CDS             complement(4806..5288)
                     /locus_tag="PK502_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99235.1"
                     /translation="MKFDNDDTVTGIHSAKLIMAIIAIILIVLLIATGLSDFLLRTTG
                     IDRDWAIAIILLLYILFSGYFYLRRASYFSYNDEGKKIIIRYFLLRPFKAPKISIEVD
                     KSEFYRFTIEKKFLRRELSIFVRNGNKVSRYPAISISSLTGKQLEQLVAALNSYVKGK
                     "
     gene            complement(5460..6608)
                     /locus_tag="PK502_00040"
     CDS             complement(5460..6608)
                     /locus_tag="PK502_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HPG99236.1"
                     /translation="MIALDNTGNAFQYLSNKELKRACRLFTLLSKLGGMNRSGKANSW
                     ASIPPVNFLLKNRIFDFFSGGLTLETCERVGDELWGYGVYSSPAPLVECNDKQLVFDE
                     YLRAIRIARDFPNHAFTLVKLSTLVEPRILRLVSQNAMLNHADKGIYTEFANRVRLLC
                     NEAFSSGKSIIIDAEHVDYQPAIDSIASEMMMLHNGDRATVYQTFRFDMVETFERLVE
                     QLRIASKNGYILGAKLLRGTLTHREVGESEKILFQNTQNDINHSLNEAIKFLLNNLAS
                     ASFIFGTHNHDTILYILSLMENLGVSPGDPQVFFAHHLGLADHLSFNLALSGYNVVKT
                     IPYGPKNIVEKGIVSSIIQHYSASHLREERHFLRKEVKRREKLVEGET"
     gene            complement(6805..8712)
                     /locus_tag="PK502_00045"
     CDS             complement(6805..8712)
                     /locus_tag="PK502_00045"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPG99237.1"
                     /translation="MKRILNLTMLLGLLWACNPMEDTYNNLDEARKPYKENITYTLDA
                     TDYSNASKYALADAQNHADSASAQLIKDQQAFNQRFTAKNYVGKVLANRFVALNLGST
                     ANVTYNVNLENTGYLTAYSSPTLYTLTSDNYQSVGGAVANTGYFIPSMPAEEFIPQIL
                     TAAISSPTANQQCLVSYKSSDFEPSSTTTRTDTLFKENFNENNLDKFDTVSVKGAQKW
                     AASAYSGNYFAKISGYSGGVNNENEDWLIIPAIDLSGVQSATLNFTTAWKYDGNDLLL
                     KYSTNYSGSGDPNAASWTNLSFDLGTGNFVFCPSGNVPLPKAKIYIAFVYTSTTSASR
                     TWEVDDVVITAKVLAKGKSVTADLFKNYNDYYRFTGSKWEKSKGVLCVQPFEYSLMGL
                     TGNAFTNTNPASRYIPTYLSQKYPYAQDGDTCIMVYNYPNANTKVAGKYQNSAGSWMV
                     YNGIVQQSGQFIHTGEHWIFDPTVHLTPSASDLQLLVDYVYLTYGRQYGSSYGNDEFY
                     FGASAYYKNFDLRLSTRSQYNVPGFAELATDEEKIALTWQRLQEGLTILLKLKYANDA
                     VLEVDGIPVYYWVTFNTYENDLAKKTYVGIFKCTQPAPDPIFERDQDAEDLAVSDGKL
                     LQSAVSWNREK"
     gene            complement(8836..11394)
                     /locus_tag="PK502_00050"
     CDS             complement(8836..11394)
                     /locus_tag="PK502_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013760737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPG99238.1"
                     /translation="MMKVIKKILLTVCIVGTAGVAFSQGIVKGVVVDSNSGEALVGAA
                     VAVENTTIGTLTSLDGSFVLKVPVEKGTIKVTFVGYSDKTIEVTIPSGTEKDLGQIQL
                     TASSIGLEEVLVVSSFVRDRTTPVAVSTIEPKIIAEKLGNKEFPEILKSTPSIYATKS
                     GGGFGDSRVYVRGFDSNNVGVLINGIPVNGMEDGKVYWSNWAGLSDVTSNQQVQRGLG
                     ASKLALSSVGGTINIITRSTDAEKGGSVYAGIGNDGYMKQTFTVSTGMLDNGWAMTLS
                     GGHTYGNGYVQATNFEGWSYFVNVSKKINSKHSIVFNAFGAPQWHNQRSNKHTIQEFR
                     DHPMEAKWNSDFGYRNGDIYNIGYAYNYYHKPQISLNHYWKISDNAMLSTQAYASIAT
                     GGGRRVYGPNSTWLSLNYPSGTPYDNSALTPEGYFDFDWVIAQNAASTTGSKAIIANA
                     INDHQWYGVLSTFTTSISDFKITAGFDGRYYSGVHAYKIDDLLGGVYYLDNKNINRPA
                     SQPLHKGDYVNYNYRGQVLWLGLYAQAEYIKNSYSGFLSVAAASNNYRRVDFFQYAPG
                     NQKSPWHDFLPWNIKGGFNYKISESQNVFINGGYIKKAPIFSNSFLNNTNDFNKGVKY
                     EKIITAEVGYGFNLRNFNAKLNVFRTSWLDKGLVRSLNGQTANILGINALHQGVELEC
                     YYKPINTLNVKGMFSYGDYRWTDDVNFTLYNEDQSVAGTYNAYIADVHVGNSAQITAA
                     VGVDYEVLPKLKVGVDFNWFGKNYADFDPTARTTIESKVDAWEMPDYYLLDIDANYRF
                     KMGSFNATIYGNVSNLLDTEYIADATDGTYHDEFSSYVFYGFGRTWSAGLRINF"
     gene            complement(11983..15264)
                     /locus_tag="PK502_00055"
     CDS             complement(11983..15264)
                     /locus_tag="PK502_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPG99239.1"
                     /translation="MKKIRFFIAMLFTVVGLWANAQVTTSEINGKVVDEKGMALPFAT
                     VVAIHEPSGTQYVTTTDNAGNYRLTNLRVGGPYKLSISFVGYSTGVYTDINLNLGESY
                     LQNVKLKESTTSLEEVVVSAGMGNPILSSERTGAQTNISRGDINTLPSISRSITDFSK
                     LTPQAQGNSFVGRDGRFNTITVDGAAFNNNFGLSSNPLPGGNAQPISLDAIDQVSVNI
                     APFDVRVSQFTGASINAVTRSGDNSFKGSAYSYIRPKSFTGDKVDGNDVEGANDRNSQ
                     NFGLSFGGPIIKDKLFFFINGEIENEDIPGVTWKPSTDGVANPDQMISRTTEADMIRV
                     RDHLINTYNYDPGKYKNFDPFQNKNTKILARVDWNISKNHKFTIRYNDVVGTSDVETN
                     FNSGPPNNARNSGRISSQSLAFSNSFYGFENTVRSITGELNSTFLTNISNKFLGSYTH
                     IQDSRTSPSKLFPFVDIWEDGDQYMSFGYELFSYNNDVINNTLSLLNNTTINLKKHTL
                     TLGVSFDRLYFKNSYIREGTTYYRYASVDDFINGADPIGFGVTYGYNGEDAPGAELTF
                     GMGAFYAQDEWKVNTNLKVTYGIRLELPFYFDDMKDNPAISALTFANSQKIDVGSWPD
                     PQLLFSPRLGFNWDVKGDRSLQVRGGTGIFTGLLPFVWFTNQPTNSGMIQSPEIGWGP
                     GNANLVDLQFNPNFKEFIASNPPLFPQAPGTLPNNAGLAEVSKDFKMPQVWRSNLAVD
                     VELPYNFVFTGEALFGKDINAVQQININEANPTGNMVGPDNRPFWATANDRKVVSTIA
                     NAVVLSNTDKGYQYSFTAQLTKNFSKGFSGMFAYTYTMAKDISSNPGSAAYSAYSSNT
                     AVGSLNNPGLSYSNFATPHQLIGHASYRIEYGGMFATTISLAYRGFQQGRWSYTYSND
                     LNNDGISSDLIYVPKDKDEALAMFVPYTPTGGVEMTAQQQADAFWAYVNGNDYLKDRK
                     GKYAERFGDVQPWLHRFDVKVLQDIFSNFGTSRRYTLQISLDLLNVGNMLNDKWGAYT
                     YNPLASYDNVRPLTRVALPTATTAPTFRLNANTIEEFETKTTLSKNISTTSTWGALLG
                     IRLIF"
     gene            15750..15983
                     /locus_tag="PK502_00060"
     CDS             15750..15983
                     /locus_tag="PK502_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99240.1"
                     /translation="MLGVLKYANSSPSYLFATALIASACRPQPPPIGRSLPATLIVPA
                     QPLPCHSAGSHQPTGSIAEVKFRFLTRRLPVVN"
     gene            complement(16224..18341)
                     /locus_tag="PK502_00065"
     CDS             complement(16224..18341)
                     /locus_tag="PK502_00065"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG99241.1"
                     /translation="MRKLISAILAILPMFTTFGQRSDIDSLLKQLNEAPNTSVKAETA
                     IDLAQRYINIDTDSAMAFNQIAHQLATDLSDTALMIRCENTYGFIFNVNSSFEKARER
                     FSRVLEMAISAHDSSLIASAYGNIGNTYMFEQKYSMAVESFLKALDIFEKRNNQKGVA
                     TVYGVLGNLYLSLDKLDQSLEYYQKAQSMFHTLGWADYEATALMNIGVIYKNKKDYKK
                     ALEYFSRAQKFEINQGNYYKSAQCLANIGEVEFALKNYEKSIAYQEKAFDAFEKVKAK
                     NDAALCLMSIGCAYDSLNKSAMAKSYYAKTLSYIDSLNQPNLKMTLAGQIYSLNKKLN
                     NFEEALKYYEIYTMWKDTILARDNQKNLAELLTKYETVQKEKEIELQKERVKRRDIIL
                     LSELLLSVLLIGLVGVLVSSYRRKKRDNEILEQSNSLISQQKEEIQTQRDEVVSQRDR
                     IIRQNRLITDSIEYARHIQSTILPSREQLSAFFPEFFIFYLPRDIVSGDFYWFKLQEN
                     AGILAFADCTGHGVPGAFMSILGITLLDDIVTPKSSISPSQILEELRVNVKNSLKQSG
                     KRVETKDGMDMAICRFNPASTYLEYSGANMPLTIIRNGEVLEFRPTRNPIGYYPKEMP
                     FENHQVELRKHDMLYLFTDGFSDQLGGPNYQKYKRQNLINLLKSIAGLSMTQQENILA
                     ASHSDWKGTYEQVDDILVMGIKF"
     gene            18648..19694
                     /locus_tag="PK502_00070"
     CDS             18648..19694
                     /locus_tag="PK502_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPG99242.1"
                     /translation="MKKVTLNDIAKKVGVSKTLVSLVVNGKARGYGISGQTCDKVMAA
                     VRAYGYNPNQTARSLRTGMSNTLGLIVSDISNNFYASLARQLEDLAWELGYNMIIGST
                     DESVGKELKLLEIFRNRQVDGIILSSSQQNPTELETLVKSGFPLVLIDRYFPTVPIPS
                     AVVNNKVGGELLAQHLLNQGFRNPVVLSLTPSYISSVAERVEGFVSVFENAAIHTEVH
                     NIPFLCLVDMVEDFFTTRLHDKNMPDAVFAVNNNLATATLRACHVHNIVIPKDIALVC
                     FDELPYFDFSKPSITSIRQPVAELCSVAFSLLLGQITNNGETKPGHVEMMPVELLVRE
                     SSTQISDNNGWRSP"
     gene            20052..21200
                     /locus_tag="PK502_00075"
     CDS             20052..21200
                     /locus_tag="PK502_00075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG99243.1"
                     /translation="MNRKTFTILLAFLCMGFGDAVGPFVGLAKEQFELSNFEAQLIAF
                     MGFIMFGFLSVPMGIFQDRRGKKVVLMLGLAVALAGLTIPLFGLGSYALFLLTILLLG
                     AGAAILQVAGNPIMRDVSEPGMYSRNLSFGQFIKAIGSLSGSIIPFAAAKWMGADWQV
                     LFPIYVSALLLTLVAVGFTKVEEEKSDKKPATLNSCFSLLKNRFVLLMVLGIFIYVGI
                     EICLSSGVPILLSTHFGIDLKTWGLLGNAFFFIAILIGRLLGSIILSWTKPAAFFRVS
                     CLIALMGILLIFSNNQITTLVGIFTTGLGFANIFPLIFSITVDAMPERANEISGLMVT
                     AIAGGAFIPPVMGLVADMSSVRTGFLVPLLGLGFIIVLSFLKPQPSRQ"
     gene            21197..22324
                     /locus_tag="PK502_00080"
     CDS             21197..22324
                     /locus_tag="PK502_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPG99244.1"
                     /translation="MSYFDDSRIVLTLDAGGTNFVFSALQGIKPIADTITLPSNGNNL
                     EKCLQGIVEGFTKLGAKLKGKPSAISFAFPGPADYPKGIIGDLGNLPGFRGGVALGPM
                     LQEKFGIPVFINNDGDLFAFGEAIAGFLPWVNSMLKNAGSPKNFYNLLGVTIGTGLGG
                     GLVTNGKLFTGDNSAGTEVWLLRDGVNPTCFAEEEISIRAVQRTYHAHTRKNESTLLS
                     PKEIAEIAYGNHEGDMVAAKLAYQSLGASLGDALANIITITDSIVVVGGGLSNAYHLF
                     APAMLAQLNGSISTVTGEQVNRLESKVFDLENEGQCNLFVKGENRKIKVPLSSKEVDY
                     DPLKRVGIGVSRLGTSHAVAIGAYVYAINMLDHNTLHSQAI"
     gene            22530..25481
                     /locus_tag="PK502_00085"
     CDS             22530..25481
                     /locus_tag="PK502_00085"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPG99245.1"
                     /translation="MKKPQLLTVVMLISIELFSQVKDPVDYVNPFIGTDFHGHTYPGA
                     TVPFGMVQLSPDTRLTGWDGCSGYHYSDRVIYGFSHTHLSGTGASDYGDILIAPVSGK
                     PVFENTRYSSPFKKENEMAVAGYYSVFLDKYKVQAELTATRRVGFHRYTFKNPNKAAL
                     VIDLEHRDRVIESWLKVVGKNELHGFRRSSHWANDQQLYFVIRFSQPFEKGPNFVDAS
                     EQLSITNGENGEVLAEGTRLKAWLLFPKLPNGKLLVKVGLSAVSEEGALRNLEAEVPG
                     WDFDSVAAQAKNDWNIELSKIKVDGGTPDQLTTFYTALYHAMVVPNVFSDVDGSYRAM
                     DNQVHHAEGFTPYTVFSLWDTYRAWHPLMTIIDTRRTTDYINTFLSDYRFGGRLPVWE
                     LAANETECMIGYHSVPVIADAWIKGIRNFDPDLALKAMLHSANLNHFGLKEYRQYGFI
                     PGDMEHESVSKTLEYAYDDWCIAQFAKSIADSSTYQKFIMRAQSYKNLFDPSTHFMRP
                     RINGGWKDNFNPSEVDQNFTEANSWQYSFYVPHDVSGLIELHGGKKNFIGRLDELFST
                     SSQLMGKNQVDITGLIGQYAHGNEPSHHMAYLYCYAGQPWKTQKLVRKIMDEMYTSKP
                     DGLCGNEDCGQMSAWYILSAAGFYPVCPGSTQLVIGSPLFSKITFNLENGKYFSVVAE
                     NNSPENIYINSASLNGKPITRCWIDYNEIMAGGELRLAMSPTPNSGWGSTDADMPTSS
                     IDEHFIATAPAFNTSTKTYMDSLQLTLTTVQPQASTFYSVAEINHPEAIPIWTQGNTL
                     NLKSSKRVKAYSVLPGGESSAIVEGSFIRIEGIKNVELKSIPSPLYDGGGKLALVDGQ
                     RGSTNFRLGGWQGYQNQDFEAIVDLGHERELHYLGAEFLQDASPWIMMPRQVTFSVSS
                     DGTSYSNIGVVNNDIPDTLEKPIVKELGVAVNVEARYVKVFAKSYGKLPSSHESAGES
                     SWLFIDEIVIR"
     gene            25676..26074
                     /locus_tag="PK502_00090"
     CDS             25676..26074
                     /locus_tag="PK502_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99246.1"
                     /translation="MNNDFICPKCEGYLVVGGSVILSISMQSGLRGLILLSPELGNYA
                     KVSSPHVKIENGEMLDYYCPICHAKLAASDVHPRLVRLIMIDDKSKRHEIFFSGIVGE
                     HCTYKISETVYEKFGESSATYDQYFKTRRI"
     gene            26572..27945
                     /locus_tag="PK502_00095"
     CDS             26572..27945
                     /locus_tag="PK502_00095"
                     /inference="COORDINATES: protein motif:HMM:NF018583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HPG99247.1"
                     /translation="MITVIIPTLNEQNTISQVIELVEKSANVSEILVVDDKSLDSTVK
                     IARAKNVKIYTSTKLGKGSSMYEGMLLATNGIIVYLDADITTYPENIVNLLTDPIINN
                     TADFVKATFNRQAGRVTELVAKPLLSLLFPELAHFSQPLSGMIAAKKSILEKATFEND
                     YGVDVGLLIDMFQEKARIVEVNIGYIENRMQSWEQLAKMSKEVSRAILKRAELIKHSN
                     LETLGNFAMIRDQMDFAIKESLLGLNKMLIFDMDNTILTKSFILTLATNYKFKEKLIE
                     IVAENSSPYVRTKLIAQLLKGLKINELLETLDGIPTVNDFERIVKEFKQKGYICGIIS
                     DSYDCITNHLVNKFDLDFSIANELEFSNGMATGEVKIPSAFLRTKDSTCNHDFCKSNA
                     MREVAHRYHIDLHNIIAVGDDENDICMIKDCGIGVAFCSSNSTLNFIADRVIQERTFA
                     PLIELLK"
     regulatory      28197..28287
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            28408..30861
                     /locus_tag="PK502_00100"
     CDS             28408..30861
                     /locus_tag="PK502_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPG99248.1"
                     /translation="MKKFFLLLLMQLAAAWAFGNPTDFSGSLKVSGRVTDSEGNPLVG
                     ATVALEGTLLGTSTNPDGSFVLNRLRPGTYVLIASFVGFETARLNLVLQENVVINITL
                     KESTLMFDEVVVSATRASGRMPIAQTTISKEQIDGNNTGFDVPYLLELQPSVVATSEG
                     GTGIGNTAIRIRGTDMSRINVTVNSVPLNDAESQGVFWVDLPDFTASVDNLQVQRGVG
                     TSTNGAGAFGATINFQTVSLSQTPFTSFDIFAGSYNTWKLSARFGTGLVKEKFSFEGR
                     YSQLGSDGYIDRAGSDHRSMFMTAAWHGAKSLIRVNIIHGEEHTGITWEGTPDYMLQI
                     NRRYNPAGEYTDEFGNTRYYKDQKDNYWQTHYQLISTFSLGSNLTLNATVHLTDGKGY
                     YEEFKEDRKFSKYALPNLVLGIDTIRTSDIIQRKWMENQFYGAVASVNYRKKSLEVAV
                     GGGWNRYDGDHFGRILWSAYNYGLPKDYEWYRNNGLKTDWNIFAKSVWQPGKMLSLFA
                     DIQLRGIQYDLKGPDSDLLPVDQEHSWHFVNPKIGTTFRFSPASELYVSFGVANREPS
                     RSDLKDALKGATGIAPKPERLYDWELGYGLRHQDFAINTNIYYMKYRDQLVLTGELTD
                     VGYALMDNVPDSYRAGVEILLGLKPFQWLNVEANTTFSRNIIEHYTNYVNLTDNPTDW
                     NETGLTEAEYLGNTTISYSPSVVGSAKITVTPVKALYFSWIGKYVGKQYIDNTESSSR
                     SLDAYFVNNIVGEYKFSPKMAKSISLQVVVNNLFNLKYEANAWVYRTKFQSGDPEYVS
                     IGYYPQATRNFMLRLAVGF"
     gene            31134..31757
                     /gene="pnuC"
                     /locus_tag="PK502_00105"
     CDS             31134..31757
                     /gene="pnuC"
                     /locus_tag="PK502_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HPG99249.1"
                     /translation="MSWLIGNYIELLGATIGLIYLYFEIKQKIWLWPLGIATSALYIY
                     IYFSSKFYADMGLQVYYLIISIYGWYHWLHGNVDNTSSSKPLPVTRITLRLLVVLTTI
                     SLLLFVILWFVLDRYTDSPVPVGDAFTTALSITATWMLTRKIIEHWWVWIVVDGVSMG
                     LYIYKGLYPTTVLFAFYTILSVVGYYQWKREITPSQVKTTAGSSAKE"
     gene            31792..32406
                     /locus_tag="PK502_00110"
     CDS             31792..32406
                     /locus_tag="PK502_00110"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HPG99250.1"
                     /translation="MNAVIVANGKFPEHPKPLRLIHEAKTLLCCDGSIEHLVKLGITP
                     TAIVGDLDSVSPELRERFKNILFHDPDQNSNDLTKAVSWCAERNINSISIVGATGKRE
                     DHTVGNIGLLVRYARMGLQVEMVTDTGTIKPLLESAKLTSFPGQQISIFSPVNQTTIT
                     TNNLKYPIDNGQIPEWWMGTLNESLGSWFELNFSPGPLLVYQMY"
     gene            32526..33602
                     /locus_tag="PK502_00115"
     CDS             32526..33602
                     /locus_tag="PK502_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HPG99251.1"
                     /translation="MKYLNSVITGTGSYIPSVKISNREFARNTFFKKDESAIETLVDE
                     VIAKFKDITGIEERRWVTDDLCASHIAAIAAEEAIATAGIDRETIDQIIVSHNFGDVL
                     QGTIQSDTIPSVASRVKHLLKIKNPACIPYDVIFGCPGWVQGVIQADSYIKSGLAKRC
                     LVIGAETLSRVIDKHDRDCMIFADGAGATIVEAVEADEKAGVIASSMMSHTEQEVFYL
                     SLGKSFHPDSDSRIRYIKMDGRKIYEYSLINVPGAMREAIEKSGINPSELKKIIIHQA
                     NEKMDEAIVARFIKQLKIQTPISDLMPMSIKELGNSSVATIPTLYDLILKGKVPNHTI
                     AKGDIIMFASVGAGMNINAIVYRQ"
     gene            complement(33690..35273)
                     /locus_tag="PK502_00120"
     CDS             complement(33690..35273)
                     /locus_tag="PK502_00120"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HPG99252.1"
                     /translation="MEKLTKIAACLAGLVILIAVATGCHEREQAKVGETEYLEEITIK
                     QLQQGYREGKFTVAQVVNDYLTRIEELDKNGPVLNSIIAINPEAMRIAQELDSELAAG
                     KSRGPLHGIPVILKDNIESKDSMPTTAGAVVLKNSYVWRDSHVAKKLREAGAVIIAKA
                     NLSEWANFRASLSSSGWSGVGGQTRNPYDTERNPCGSSSGSGVAVAANLCAFAIGTET
                     NGSIVCPSNNNGIVGLKPTVGLISRTGIIPISFTQDTPGPMARTVEDVAVCLGIMVGV
                     DSTDAKTLNPDAVFYSDYSKFLKRDGLKGKRIGLLTNVSGFHFKVDTLMKQSVDAMKQ
                     AGAEVVAIDFPVSREVDNASFEVLLYEFKDGLNRYFASLGESARVKNISELIKFNLND
                     SVELMYFDQQLLEMAQSKGDLNSPEYKKNLEMMQTGMRKQGIDRIMNEYKLDALVAPT
                     GSPAWKTDLVDGDHFLGGSSSYAAIAGYPSISVPMGFIDGLPVNISFIGKAWSEAELL
                     SIAYSYEQATHQRKKPMLE"
     gene            complement(35522..36409)
                     /locus_tag="PK502_00125"
     CDS             complement(35522..36409)
                     /locus_tag="PK502_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99253.1"
                     /translation="MENNEKSAKTIKTLRVIMVMLLLIIAGLTVVYFQQKQQAKDTQE
                     ILQAQKDSISNNLLRLMNDYSELETTNDTLNQKLQKEQEHAKKLYAELQSVKQISYAK
                     IKEYQKELGTLRSIMKDMLHDIDSLNALNKELIAENVKVKEEAATAKQTVIELEQKTE
                     ELNTQVAKGSIVKARDIVAMGVNQKGNEVTRARRVNKIRACFTLSENSIAKPGTRNVY
                     MRIVGPDDFVLTKSEADLFDFEGEKIVFSASREVDYQNSDLDVCIFFDNNGELLTGVY
                     KVTLYMDGYMIGYSEFSLK"
     gene            complement(36449..37372)
                     /locus_tag="PK502_00130"
     CDS             complement(36449..37372)
                     /locus_tag="PK502_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG99254.1"
                     /translation="MSIGISHITKQFGEQKALNDVSFNISTGQVVGLLGPNGAGKSTL
                     MRIITGYLFQDSGSVDINGHNTQDTRFDFRREIGYLPENNPLYTDMYVKEYLSWVASI
                     YKLGKEKRRRLSELIGITGIGDEQGKKIGSLSKGYRQRVGLAQALIHNPSVLVLDEPT
                     SGLDPNQIVEIRNVIEEIGRDKTVIFSTHILQEVEAICSRIIIINKGLVVADGTNTEV
                     KALSSGLRQNIVVEFAEKVSREAIMAIEGVETATLIDTNCWWITSGRLEDLRGKIFNF
                     AKNAGFTLLTLQKRDLQLEEVFQNLTLGNRN"
     gene            complement(37433..38704)
                     /gene="metK"
                     /locus_tag="PK502_00135"
     CDS             complement(37433..38704)
                     /gene="metK"
                     /locus_tag="PK502_00135"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPG99255.1"
                     /translation="MGYLFTSESVSEGHPDKIADQISDALLDEFLRRDSEAKVACETL
                     VTTGLVVLSGEVKTKAYVDVQEVARRVIKRIGYTNDQYRFDSESCGVISTIHEQSPDI
                     TRGVVRQSEEEQGAGDQGMMFGYACRETDDYMPLALVLSHVLLMELAGIRREGRQMTY
                     LRPDAKSQVTLEYDDNNQSVRIHTIVVSTQHDEFDTDDARMLSRIYEDVKNILIPRAK
                     KRFPERVQKLFKDDYILHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFS
                     GKDSSKVDRSGAYAARHIAKNMVAAGIADEVLIQVAYAIGVARPINLYVNTYGTAKVS
                     LSDGEIAQKILTIFDLRPAAIVKRLGLKNPIFEETAAYGHFGRDHYIRNVDFIENSNC
                     NGEKIVKREVEFFTWEKLDYVDRLKSEFGIK"
     gene            complement(38960..39358)
                     /locus_tag="PK502_00140"
     CDS             complement(38960..39358)
                     /locus_tag="PK502_00140"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPG99256.1"
                     /translation="MMAKALKFFSKNRNSILWGLVILILCTMPPSGFPKVSVSGIDKL
                     VHFGLFAVLTLLVISENNTLRLSGSVLQKTRWVGIVVSIMYGGLVELLQLKVFTYRGA
                     DWFDLIADVVGTLIAAWLYLPISRLTRRLV"
     gene            39697..41085
                     /locus_tag="PK502_00145"
     CDS             39697..41085
                     /locus_tag="PK502_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HPG99257.1"
                     /translation="MFDKQIYVDRRLKLRKKVKSGIVLILGNTESPINYQGNTYNFRQ
                     DSNFLYFFGLDTPGLAGVIDIEEGTDCLYGDDFDIDDIIWMGPQPKLKDMGEKVGVLS
                     THPFRELSTTLNRAIRQGRKIHFLPPYRADNTLLLEKLLGINQTIIKTYSSIELIKAI
                     VSLRSVKEPCEVEEIEKACSVGYKMHVTAMKMAKPGALEREIAGELEGIAHSFGYMTS
                     FPTILSQHGETLHNHDHSLTLEEGRLLLVDAGAEGISHYASDFTRTFPVGGHFSQKQK
                     DIYSIVLAANNKTTELAKPGVPYFNVHKAAARVIVEGLSALGIMKGNSDEAVENGAHA
                     LFMPHGLGHMMGLDVHDMEDLGEDFVGYDDEISRSEQFGTSSLRMARKLQKGFVLTNE
                     PGIYFIPALIEKWKSEKINQAFINYDVLSSYLTFGGIRLEDDLLITDNGCRILGHRIP
                     ITPEEVEKTMNS"
     gene            41616..42596
                     /gene="nadA"
                     /locus_tag="PK502_00150"
     CDS             41616..42596
                     /gene="nadA"
                     /locus_tag="PK502_00150"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPG99258.1"
                     /translation="MNYSIVDKLGYIDEDIDSNIDLKVAIRKIKEEKNAVILAHYYQR
                     PEVQDIADFVGDSLALSLKAASLNSSLILFAGVKFMAETVKILRPNSQVLIPDLNAGC
                     SLADSCQFSDFKKFIDLHPNHIVVTYVNSSAEVKTISDICCTSSNAIKVVKSLNNSTP
                     LIFAPDLNLGSYVKSTLNRSNMVLWNGSCHIHNQFMLQDIITLKTKHPKAKVIAHPEC
                     QNSILLISDFIGSTKSLLDFTYNSNNTDFIVVTEPGIIHQMKKLSPQKNFYFPYVQNE
                     ECYCNECSYMKLNNLKKIYLTLKYEYPIINLEPDVISNAAIPINRMLKIN"
     gene            42605..43600
                     /gene="ruvB"
                     /locus_tag="PK502_00155"
     CDS             42605..43600
                     /gene="ruvB"
                     /locus_tag="PK502_00155"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPG99259.1"
                     /translation="MENQISDKEFENNIRPSEFKQFNGQDDIIKNLKIFVQAAKIRSE
                     SLDHVLLHGPPGLGKTTLAYIIANELGVSIKTTSGPVLEKPGDLAGILTNLDKFDILF
                     IDEIHRLSPIVEEYLYSAMEDFRIDILIDKGPSARSIQIELNPFTLVGATTRSGLLTS
                     PLRARFGINFHLEYYDASTIKSIIKRSSYILNIDIDDDASMEIALRSRGTPRIANSLL
                     RRVRDFAQVVGNGRINLDIAKSSLDALKIDSLGLDEMDNKILTTIINKFNGGPVGINT
                     IATAISEDPGTIEEVYEPFLIKEGLLKRTPRGRQTTDLAYSHLKIEKNFRQGTLF"
     gene            complement(43592..44233)
                     /locus_tag="PK502_00160"
     CDS             complement(43592..44233)
                     /locus_tag="PK502_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPG99260.1"
                     /translation="MTTEESEQLEANLECYEYKKGEIIYKEGSRITGFYCVKKGVFKI
                     YKTGIDSKPQIVAFAQKGDITGYRSVLSNEPACTTVESLEDSEVCYIPSSLIFEFIRK
                     NSNFALSLIQLTCKELNTANDLIKDIAQKTVRERLAETLLMLDQTFGKDEEGFINVNL
                     TREDISSIIGTATESVIRILSEFKTDGLISLKGKKIGIGNYSKLESISNSFLK"
     gene            complement(44322..45341)
                     /gene="pheS"
                     /locus_tag="PK502_00165"
     CDS             complement(44322..45341)
                     /gene="pheS"
                     /locus_tag="PK502_00165"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPG99261.1"
                     /translation="MQEKINNILELIDKFTPQNYEEVESFRISLIGKKGELTKLFDDL
                     KNLGTDEKKYYGKLLNDLKKKLNEKLDILKDTYKSKGKKEDKIDLTLQEPQLNFGTLH
                     PITIVKNEIFNIFRKIGFSIYEGQEIEDDWHVFSALNFPPEHPARDMQDTFFIQKNPD
                     VLLRTHTSSVQVRSMENEKLPIRVICPGRVFRNEAISSRAHCIFHQVEGLYIDKTVSF
                     ADLKQTLEFFVKEMFGSKTSIRLRPSYFPFTEPSAELDISCNLCGGKGCSVCKHTGWL
                     EILGCGMVDPNVLELNNIDSKKYSGFAFGMGIERIAMLKFRVNDLRNYFENDIRFLNQ
                     FTNFY"
     gene            complement(45361..46173)
                     /locus_tag="PK502_00170"
     CDS             complement(45361..46173)
                     /locus_tag="PK502_00170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPG99262.1"
                     /translation="MNKNILLYFSIAALLLLTGCKNATVDNRHLVKISTNYGDIEVVL
                     YNETPKHTLNFINLTDQHFYDGQIFHRVIKNFVIQAGDPETKNSQPGKEYGEKDSGYL
                     IDPEFCDTIIHKYGAIGMARESDDINPDKLSSGSQFYIVIGKVYTNEELDNLEKKINF
                     EKLNKIKQDLYKKYLNENTAVSDKNIKDILILIENMVDSVKSSEKPFIFTNKQREIYT
                     TIGGIPHLDGNYTVFGEVSKGMDIVKKISEVKTDQNDRPLVDIVINEVKKIR"
     gene            46296..47789
                     /locus_tag="PK502_00175"
     CDS             46296..47789
                     /locus_tag="PK502_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPG99263.1"
                     /translation="MKYFNFVRQILFMASVRQLYSQSVVYGLSTVVPRLLNYLLVPLH
                     TTVLTNPEQYGVITEMYAYISFILILLTFGLETGFFRFSSKFKNNESVYSTIFYFLIF
                     TSSLFFLIISIFSLDISNFLGSFYKPIYITTLAGVVSIDAVLALPFAKLRNENKPFLF
                     SSLKILGVVINVFCNILFYLIIPRNSLYTLFPNVDLLYFVFLSNLIQNIFVLILLLIF
                     TKIPKLIFSPKLLKSILIYSLPLLIAGLSGTTNEAFDRIFIKYLYPIKDEALYQLGIY
                     GANVKLAVFLVLFIQMYRFAAEPFFFKLQNESNNKEIFAKSFKYFLIFCFFITILITF
                     NIPILKYFVGSNYRHNLYIVPILLIANSLFGIYFNLSFWFKLSDKTLFGLKYVGIGAF
                     TTIISNVILIPLIGIFGAAVSRIISYTIMTLLCYIDGKRSGYISIDRNNLKIFLLFLL
                     FISFISTVLLIYSKPLFSLIFANLFLILFIFFFLKKENIKILNLRKC"
     gene            47783..48217
                     /gene="dut"
                     /locus_tag="PK502_00180"
     CDS             47783..48217
                     /gene="dut"
                     /locus_tag="PK502_00180"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPG99264.1"
                     /translation="MLKVKIVNKSSFPLPGYATSHSSGIDLYANISSSIKILSLQRIL
                     IPTGIFVEIPDGYEAQIRPRSGLALKHGLTVLNTPGTIDSDYRGEISIILINLGSDEF
                     ILEPGIKIAQMVFSRYERVHFIEVNELTETSRGAGGFGHTGI"
     gene            48293..49474
                     /locus_tag="PK502_00185"
     CDS             48293..49474
                     /locus_tag="PK502_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPG99265.1"
                     /translation="MKYIFKYCLFFILCYCITFPIKSQSLEDLNSTKRKIENQIQQLN
                     VLINENANLKSETINNLNLVSQRIKLKNSLINQLELEILYLNNNILQSQSNIDSLSFL
                     LNSRKKELEKILQFYQKYHNSYNILIFILSSNSFNQSFLRIKFYNSIIKYYSNTIKSI
                     STDINKISEIKVSLENQKKELYLKQLQKIDEIKTLRKDLDLYNSKLKKLKSNEKNLRK
                     QIEIEKRRSNEIISKINELIEEETRKKIISTNKSNENVKLSNDFLKNYGKLPHPVANA
                     FVINNFGETNHPVLKGVKIKNNGVDFACKTGSEIRSIFNGEVKKVFNLPMNGIAVIIR
                     HGNYLSVYSNLTNVYVKVGDKVITEQVIGKIVSNDNSGSILHFEIWNEKNPENPLMWI
                     Y"
     gene            49534..49929
                     /locus_tag="PK502_00190"
     CDS             49534..49929
                     /locus_tag="PK502_00190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG99266.1"
                     /translation="MQTIIELNSSLDSISKVEKFIDEISANVNISSEIYGKILIATIE
                     AVNNAIVHGNKLNSNKIVRVKIESEGNRLKVIVEDEGPGFDFYNLPDPTTPQNIENIS
                     GRGVFLMRKLSDEINFFNNGSLVELIFYL"
     gene            49929..50351
                     /gene="ybeY"
                     /locus_tag="PK502_00195"
     CDS             49929..50351
                     /gene="ybeY"
                     /locus_tag="PK502_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPG99267.1"
                     /translation="MAISFQSNINFNLSNKRKVKTWIKNVVTKYGFKIGDVTILFTDD
                     EYIKELNIKYLNHHYFTDILTFDYSSGFVLNGDIVISIDTVRSNSILYSTSIDDELLR
                     VIIHGFLHLLGFNDKSSEESRKMRELEDGALNIFYSND"
     gene            50344..52215
                     /gene="mnmG"
                     /locus_tag="PK502_00200"
     CDS             50344..52215
                     /gene="mnmG"
                     /locus_tag="PK502_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPG99268.1"
                     /translation="MTDFFDIIVIGGGHAGCEAASIAAKLGSSVLLISLDLTKLAQMS
                     CNPSIGGIGKGQIVKEIDVLGGYTAKVTDLTTIQFRMLNKSKGPAMWSPRAQCDRMLF
                     SYEWRKYLESIDNLHLFQDEVVDIDFDVKNQLVSGVTTQSGIKFKCNVLILTNGTFLK
                     GKIHIGSNNFSGGRFGEKASIMLSDKLEDMGLHIGRMKTGTPVRIDGRSVDFSKLLEQ
                     KPDTYGKFSLDPKYTSTLQQKSCYVAHTNSVVHDIIREGFDKSPLFSGKIKGIGPRYC
                     PSIEDKVRTFYTKDSHQLFLEPEGVNTYEMYLNGFSSSLPVDIQLKALKNIEGFENVS
                     ILRPGYAIEYDYFDPMQLNHTLAHKSIKNLFFAGQVNGTTGYEEAAGQGLLAGINAHY
                     FINDSSKQLILGRNEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRILIRQDNVDERLLP
                     IAQKLKLASNEDIDRFNVKSKKLESFKNLISSITVSPQDVNDYLVKVNSSPIEHKRKL
                     NDILARPDVNFFELLDSCPNLIGILGDVDKNIAENYIIKIKYSSYEEREQFMVDKTQK
                     LEALKIPNNFNYESIRSLSTESRQKLSKVKPKTIGQAKNIPGVSPSDINVLLVYLGR"
     gene            52457..54073
                     /gene="ade"
                     /locus_tag="PK502_00205"
     CDS             52457..54073
                     /gene="ade"
                     /locus_tag="PK502_00205"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HPG99269.1"
                     /translation="MNRSGIVKGVVYNPILHESFGALVFIENGKILKIERDGNIKEPL
                     ILPGFIDSHVHIESSMLTPAQFGRTAAAHGTVAAVADPHEIANVAGVEGINFMLSSAI
                     ESPIKLFFGAPSCVPASPFDECFQPFSPGIIKELIDRSDIFFLGEMMNFPGVIGGNAE
                     VMEKINIAKSAGKPIDGHAPSLDKNDLKKYISAGISTDHESFTLEEAIDKLSFGMKVI
                     IREGSAAKNFDALHSLIPQFHDNLMFCTDDCHPDDLIVGHINKLVKKAINAGYNIFDI
                     LQVSSVNPVMHYNLNVGLLQPGDPADFIVVDGLSSLNIISTYINGDDVLKEPRPTTNV
                     KIPTYTFPNSFDSNLLVKKLDTKRVKVIDVVKDELITNWHVENSNTDLLEANPKEDLL
                     KIAVVSRYKKNEASVGLIRGFGINKGAIAASIAHDSHHIIAIGCDDNSITQSINYIVK
                     CGGGICYFDGDSIYGLPLPAFGLMTNESAEVAGKAYHELTQRVIADGCKLQAPFMTMS
                     FMSLSVIPHLKITPTSLFDVDKFSFTNICL"
     gene            54109..55905
                     /gene="uvrC"
                     /locus_tag="PK502_00210"
     CDS             54109..55905
                     /gene="uvrC"
                     /locus_tag="PK502_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPG99270.1"
                     /translation="MNDILSNIVINLPDSPGVYQFFDINGILIYIGKAKNLKKRVSSY
                     FHQSAYESAKLRVLVSKICDIKFIVVDSESDALLLENILIKKHKPRYNVLLKDDKTYP
                     WIVIKNEPFPRVYKTRKYIDDGSSYFGPYTSGRLVFELLDLIRKLYPVRTCSLNLTTA
                     NILAKKFKPCLEYHIGNCLAPCIGMQSHQSYLQNIDSIKRILQGNLSEIKNHLKAEMV
                     KYSNQYKYEEAQLIKEKIDILTNFQSKTTIVNPKYKDIEVFTILTRHNIVGVNFIRIN
                     KGQVVQSYNLHLKSFVNESLPELFSSAIAEIRQRLKANSTRILVNIMPEFTINGVSFS
                     VPQRGDRKKFVELSLRNLEIFLDDLYKNEAAKSPVLAVDSILTKIKDDLNLSDLPVHM
                     ECFDNSNIQGSNPVASCVVFKNAKPSKKDYRHYNIKTVQGPDDFASMKEVVYRRYSRM
                     LSDGETLPQLIVIDGGKGQLNAAMESIVELGIQNRVHLIGIAKRLEEIFVPGDPVPVY
                     LDKRSETLRIIQQIRNEAHRFGIKFHRNKRSNSAFESSLEQIEGIGPKTIQLLFTRYL
                     SLDGIRNAPESELIDLIGTKRTNLIKNYFKKV"
     gene            55972..56817
                     /locus_tag="PK502_00215"
     CDS             55972..56817
                     /locus_tag="PK502_00215"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HPG99271.1"
                     /translation="MDSLNLRINLYDNLKQAFFSPDTLNGGCTISPSADTALASSTKL
                     NNVVSSLSGDANVMVEPILRKTNNIPNSYIIPFLAGLVVLVVIVLRYRKYLASIVESA
                     LYSFFAEKFVLETNVPFRRTFALLDALSVVSFSCFSLVALKLLNTEFFYESKPIILIA
                     IAIASFSLFRLFISIYNYLINSILSNNPIFSLFQYNYFIFIRIVGFIILPISFVAFYA
                     PTSVGLYLLYFMFGILLVTLFYRLYRLTLIFVKNRISILYFILYLCGLEVVPILIVIK
                     EVQRI"
     gene            56842..57594
                     /locus_tag="PK502_00220"
     CDS             56842..57594
                     /locus_tag="PK502_00220"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HPG99272.1"
                     /translation="MKISRIVVAQQEPVNEKSPYLDISNRYGVKIDFRPFIHVEGISA
                     KDFRAQKINISEHTAVIFTSKTAIDHYFRMCEELRVTIPETMKYFCITESVALYLQKY
                     IVYRKRKVFFGNNTFPDLLEIIQKHKEEYFLVPLSDIHKPEIPKALEKAKLKFRKGVF
                     YKTVSSDLSDLNPFPYDMVVFYSPSAVKALFDNFPQFKQNDVVIGGFGKNTAKAIKDA
                     GLRLDISAPTTTCTSMTLAIDNFLKNLSKKSK"
     gene            57620..58024
                     /locus_tag="PK502_00225"
     CDS             57620..58024
                     /locus_tag="PK502_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPG99273.1"
                     /translation="MKYFLRKNNRLTLQHQVDLLFSHGYGYFIYPIRAKVLLYDRNVE
                     EDTWFKVMVVVPKRHVKKSSSRNLIKRRIKESFRVAIHSLKGSCPSNKIILIAISFVS
                     GDIKPYDRIKVSIEKLMNEIENDIEKLKSIKD"
     gene            58091..58327
                     /gene="yidD"
                     /locus_tag="PK502_00230"
     CDS             58091..58327
                     /gene="yidD"
                     /locus_tag="PK502_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPG99274.1"
                     /translation="MTKNKIRDVLILLPLGLIKFYQLFISPHIPNSCRYTPTCSVYSV
                     EALKKHGLFKGSWLSIKRILRCNPWGGHGYDPVP"
     gene            complement(58329..59480)
                     /locus_tag="PK502_00235"
     CDS             complement(58329..59480)
                     /locus_tag="PK502_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPG99275.1"
                     /translation="MSISGNTLKNFALIIFAGFIVIFSLLVSNKLVKNLSEEEHAKID
                     LWAKGVRFLSTTQDSCQDLTFVSEVIQNNRTLPVLLTDESGRIISHLNISERQIKDSV
                     AVYRLLRKMTSEHEPIEIMLSDSTKNYVYYRDSTTLRRLEFFPYIQIVIILLFFLISY
                     YAYSQSRKAHENSIWYGMAKETAHQLGTPTSSLMGCLALLEEKIPNDPIIMELEKDIV
                     RLEKIAGRFSKIGSTPILQEANLIELLNSTIDYLGARVLKNVTVIKKYNVQEKLNIRM
                     NGILIEWVIENIVKNSADAMMGKGEITFKVTDNTQVVFIDISDTGRGIQKGKFEEIFR
                     AGFTTKDRGWGLGLTLSKRIIEQHHGGKIFVLNSEPGVGTTFRIVLKKR"
     gene            complement(59492..61867)
                     /locus_tag="PK502_00240"
     CDS             complement(59492..61867)
                     /locus_tag="PK502_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPG99276.1"
                     /translation="MLTTYVFINQSKLIDLLLKQFNEYELVSIYYSSIKFDTFKNFPF
                     ATFSFSDFILTLPENGNNDTLMSAQNLDLKINAARLLLGNFEINSCKAENGRLNLKTN
                     LLKKNDINPANGQAALLQNEFFINRFTLSNFKALIEDTLSRQKSLIGIDRLTLNIRAK
                     KILTLNLDCQLKEINLGSKFMLNEQTQVNAIASYDGKSVFLRNVKISLSGSRLELQGL
                     YHLENSTLGLGFKLNRTNINRLIQVANIKGLPADITGKVAMSGKLSYNGGKKNLTGLW
                     IDYDAQNVSIIAKGKIIEIKEINGLSYFTNNLQTHSGQVSNAVVRYENIEAAFSGRTY
                     GTNRIIILAEGLLKLGKNEPSFTNKLTGLEGNGTFKALLELRKGNGLQSKHFGFLLHD
                     VASEISFSLDSISAFKNLSSISGKATIRDKAIISATCYLDSSKIDFTLCQNNAIYYLN
                     NPLNFSPSIYLNSSYLNLDSLIKSVSTVNKGGKKRVYTTVETSINTKKLIYTNYAFEN
                     VTGVMNIVGDSIYVKGLNAKGFEGKIDGDLMVVGDKYSINASMEGIDITELFRHYNSW
                     NQKYITHNNIEGHLSGHCNFDFELDSANNIRWDRAKMNSEITINDGKLRGMNNIKKLS
                     KWLKLDQVKSIDFTTIHNNILISNGCIYIPSMDLNSNVMGLNLSGKHFLTNDYEYHAR
                     INFSSLLAQRFLNYSADYNQPKTSSGAINLYLTLKGNSKGYEVFLDKKSSYEAIKNTI
                     NTEGQSLKEIISNEFKQTFEDSLKKQHAIQQQPTSQKFQVEWDEYDTTKTK"
     gene            62616..64823
                     /locus_tag="PK502_00245"
     CDS             62616..64823
                     /locus_tag="PK502_00245"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG99277.1"
                     /translation="MKFRIVSLCLLFAFRVGISQNIDSLLLKLYQESNDRSRIEILLN
                     ISSFYDQHDRNFIQAENYLYEALQIAQTAGDNTLLANTYNELGKFYRNQSKYPEALKY
                     HHLADSLAVKMGDCILQATTLNNIGVVYRRLDNHAQASEYHLRALNIAEGCKDTFNIS
                     VACNSLGNIFSLNGRYQQALSYFNRALALSISQNNLLGQAMNFNNIGEVYEFMGDYRN
                     AKYYYTKSLDINFKLKNLKGISINYNALGKIEMFNGNPENAYRLFQNALDIDRKLGDK
                     KFLADSYVNLAKACLALKKIDKAKIYLDSTITIAQQIGSPWHLQSAYEAYSDYFVQKS
                     DYLSALISFKNASRFKDSLLNEKNARHLSTIQTIYETEKKEKEISYLLQEQALKQKEL
                     EKNNAQKNALIIGLVLSSLVVIVSLFAWRTKVKSNIVLSDQIKEIEDKNRLLEIQKEE
                     IEQQKIKIESNQAFIEQKNKNLEEAHKIIESYIENITDSIRYAEKIHESIQPSLSDML
                     SIFLDAFIYSKPKDIVSGDFCWLTRRDNLVFLALADCTGHGVPGAFMSTIGINILNQA
                     INEQHLYSPDKILTFINRTLINRLNKSTGEQVLKNSMDIAVCTFDTESLLLSYSGALI
                     PIFIQKGNSVEEVKPDSITLGTNFSHSASGFSLQNIQLNHGDWVYLSSDGFFDQLGGE
                     SRKKFTRKQFMQIVADSPNMKGNVKVDIIEKKFLQWMNRTPQLDDVLVWGFQV"
     gene            64858..65547
                     /locus_tag="PK502_00250"
     CDS             64858..65547
                     /locus_tag="PK502_00250"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPG99278.1"
                     /translation="MKIPDTTSLQLKISNTHTINMNENRSIERKRFCISLFVSLIFIV
                     ALWCIKIFEIVEGISLYRLGVFPRQISGLPGIIFSPLIHSDFNHLISNSLSLLFLLIG
                     LLYFYRDLSYKVILFIWITSGLMVWVGARDSYHIGASGLIYGLASFLFFSGVVRHDQR
                     LMAISLIVVFFYGGLIWGILPVYPAISWEYHLFGGISGLIAAILFRNQGPPKKVWSWE
                     QEEDENTPENL"
     gene            65839..66789
                     /locus_tag="PK502_00255"
     CDS             65839..66789
                     /locus_tag="PK502_00255"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPG99279.1"
                     /translation="MESHIIINQLLAFIVLAVIGAIASWRKIINDEGKDFLSRLIIDV
                     TLPLLIVSTFLKLESNPRLLVNGLWLFVLTFVNIGVLYMAGTLSARAQKLSPSDSTVH
                     VLHTMFGNIVFLGFPLFDALFPNGVGVFYASVYQLASNFITYTYGTYKLSAGVQKSKL
                     GNFFNINTIALLLGILLMVLKVKLPAFILEPISSLGKCTSPLSMVYIGALLAGMNLKS
                     TFKTTSIYVLTINKLIIVPLLLGFIYSFLRRSFNLNLSIEAFVVLVMQAAMPCQTIVV
                     VMSRRYGGSHVLATGNLFISTIFSIISLPLVYHFLVSIYS"
     gene            66924..67379
                     /gene="dtd"
                     /locus_tag="PK502_00260"
     CDS             66924..67379
                     /gene="dtd"
                     /locus_tag="PK502_00260"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPG99280.1"
                     /translation="MRVVIQRVKSAKVEVNNHEVGCIHKGLLVLCGFEVEESEEDLQW
                     MASKICKLRIFDDETETMNLSIKDISGEILVVSQFTLHASTKKGCRPSYVRAAPSSLA
                     ILFYNRFLNLLEIELGSKIAAGVFGKMMEVSLVNSGPVTIVIDSKNKEF"
     gene            67382..67708
                     /locus_tag="PK502_00265"
     CDS             67382..67708
                     /locus_tag="PK502_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HPG99281.1"
                     /translation="MTFEEAQKEVDSWIRDHGVRYFSELTNTVLLMEEVGELARIIAR
                     KFGEQSFKDNESADGLSDEMADVLFVLICLANQTGVSLSEAFEKNIEKKTQRDSQRHK
                     NNPKLF"
     gene            67747..68622
                     /gene="deoC"
                     /locus_tag="PK502_00270"
     CDS             67747..68622
                     /gene="deoC"
                     /locus_tag="PK502_00270"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPG99282.1"
                     /translation="MDFFVEDKFNLEVNGEEVGRHLARIKDLALSRLGDTDKLKACFG
                     SIDLTTLNTTDTAERGKQFAEKVNGFTVDFPEMPSVAAICVYPTLVSSVKENLKAKGV
                     KIASVAAGFPSSQTFLEIKLSECRMAIGQGADEIDVVIPVGSFLQGDLYSVFSEVKQI
                     KETAGDIHVKVILETGALPSLKEIKLASFLAMEAGADFIKTSTGKLEPAATPEAVFVM
                     CTAIKEYFEKTNRKVGIKPAGGVVTSSEALVYYTIVEQVLGSSWLSPDLFRLGASRLA
                     NNLLSDIYGRAVSYF"
     gene            complement(68725..69627)
                     /locus_tag="PK502_00275"
     CDS             complement(68725..69627)
                     /locus_tag="PK502_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPG99283.1"
                     /translation="MKPTLIILAAGMGSRYGGLKQVDGLGPYGETIIDYSVYDAIRAG
                     FGKVIFVIRRSIEEDFMKIFGNRFSRDVNFDIVFQELDMLPDNITCPPEREKPWGTAH
                     AVWVARKMVNEPFAVINADDFYGYDTFKTLANELSRSNLKNGQYFMVGFRLNNTLSEQ
                     GSVSRGACEVDNASFLKSIVEFTRIQRVDGKIFNIDDSGNRIELDGSMLVSMNFWGFT
                     QDFFTLTETLLKEFLNGIPDHKKSEIYIPTVVNRAIQDGLATCKVLSTTSEWFGVTYP
                     GDKIHVVDQLRKLTLNGVYPSPLW"
     gene            69881..71128
                     /locus_tag="PK502_00280"
     CDS             69881..71128
                     /locus_tag="PK502_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008823384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HPG99284.1"
                     /translation="MMRRSDIEVMAPAGNFESLMAAIQGGADAVYFGVGKLNMRAKSS
                     VNFLEDDLYEIVRICSAGNVKTYLTVNTVLYDEDIDDMHRLIDTAKKARVSAVIVSDQ
                     AAMEYARQVGVEVHLSTQLNISNTHSLRFYSQWADVVVLARELNLQQVKLISEGIHKH
                     NINGPSGNKVRLELFVHGALCMAISGKCYLSLHQKNYSANRGECLQICRRSYTVTDNE
                     TGSQLEIDNEYIMSPKDLCTISFLDQILDAGIRVLKIEGRARGPEYVKTVTSCYAEAV
                     EAIVSGQYTPDKVEGWTKRLTSVFNRGFWDGYYLGRRLGEWSNSYGNIATERKQYVGK
                     ISNYFSDMGVAELNVEATELYLNDKVLIIGPTTGVIETLVSEIRVDNQQASFAPKGLP
                     CSIPVPSKVRRSDKLYRIIYSKP"
     gene            71395..72018
                     /locus_tag="PK502_00285"
     CDS             71395..72018
                     /locus_tag="PK502_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HPG99285.1"
                     /translation="MVKNFSELCNNIFDECTKYYHTMDNVDANPNVPYKSNTIEHSLF
                     IKNWIDAVQWHLEDLIRDPNINPEKALLLKRRIDKSNQDRTDLVEQIDSYFLEYFKDV
                     RPQNGATINTESPAWAVDRLSILALKIYHMEQEVSRTNASADHVATCRQKLTILLEQR
                     KDLSMAIDQLLDDIANGRKYMKVYRQMKMYNDPSLNPILYGKNETNP"
     gene            71993..73069
                     /locus_tag="PK502_00290"
     CDS             71993..73069
                     /locus_tag="PK502_00290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPG99286.1"
                     /translation="MARMKPTLKENKPKKLLVIRLSAIGDVAMTVPVLWSVVKHNPDV
                     RVTFVSQSFARDIVSQIPGIQFYEADTKNRHKGFWGIFKLFRELKNLDRFDAVADLHN
                     VIRSRVIGVLFRIGGIRYEVIDKGRKEKKQLCSVKNKKLVELKPTTERYADVFRHLNL
                     VVSSDFDYLFDEARLPDDISAYLGYKSGTWIGVAPFAKHKGKIYPLNLMEAVVSRLAA
                     NKSYKIVLIGGRNFEKELLEEWAKKYTNTFSVAGKYSILNELKIISNLDVLISMDSAN
                     MHLASLVNTPVVSIWGATHRFAGFYGWKQNADNTVEVELSCRPCSVYGNKPCFRKDYA
                     CLNRITPEMIISKVNSVVDRYGRI"
     gene            73679..74590
                     /locus_tag="PK502_00295"
     CDS             73679..74590
                     /locus_tag="PK502_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPG99287.1"
                     /translation="MSYVTKEKLFSKAWFKAYTLITIGAFVLAAGFVLFIDPHRIAPG
                     GVYGIGIIVHYLTQGVFSWAPEGFPIGTVGLLLNIPLTILGIKILGPRFGVKTVVGFI
                     LSSVFIDTLHMIYGYHPLVDDMLLSSIFGGVLIGFGLGLIFKSKATSGGSDIIAMIIA
                     KYTRLPLGQLMIYVDSTIVLIALVAFKDWKIPLYSWVVIYITGKVIDMTIEGASYDKA
                     LFIISDRYEEVRSKIINDLERGGTLISVKGMYSGEEKKLIFTNVSRREVFMLKDYIRE
                     IDPKAFITVIDANEILGEGFKPISEAL"
BASE COUNT        21872 a        15276 c        14809 g        22863 t
ORIGIN      
        1 cacggagagg tgggtgagtg gctgaaacca acggtttgct aaaccgtcgt acttcgcaag
       61 gggtaccggg ggttcgaatc cccccttctc cgcaccatcg gggtgtggcg cagttggtta
      121 gcgcacctgc tttgggagca gggggtcgtg cgttcgagtc gcaccacccc gaccaggagg
      181 cctaaacggc ctcctttttt ttgcttttat cgctgcatgt tccattgttt atgtaaacaa
      241 gcaactcgat gaatactagt tatttatgtg gatagccatt gccatacatc cagctggtgt
      301 cagagcctgg cttgcttcaa tctgataaaa aaacgatatt tgtcatgctc ttttagcgca
      361 acaccatgac tgctgttttt ttaacctgtt accattaacg gtaacagaac atgtgtcatt
      421 taagcaatgg ttcatgaaac aaaggaaaaa caaggatgat gaaagtgcgc tttttgtggc
      481 tccaggaatt gaccagccgc ctgttgtaaa cgagcaggcc gttcaaaagt ttaggcagca
      541 aaagcgccgg caaccgagta tagacgagtt tgttggcgga atacttgctc gcaacaacac
      601 catcctgagc caggcaatta cattagtcga gagttcacta ccacgacact acgagctggc
      661 ccagcagcta attgaacgtt gccttcccta ttcgggtaat tcgattcgtg ttggaattac
      721 aggaattcca ggggttggca aaagcacatt catcgaatcg cttggaacaa tgctaacatc
      781 gcagggacac aggttggctg ttcttgccat tgacccgagc agtacgcgaa gcaaggggag
      841 cattcttggc gataaaaccc ggatggaatc gctcagcgcc gatgccaatg ctttcattcg
      901 cccttcgcca tcagccggtt ctcttggagg cgtagcacga aagacaaggg aaaccattat
      961 cctttgcgag gctgccggat ttgatacggt gtttattgaa acggttggcg ttggtcaaag
     1021 cgaggtttcg gttcactcca tggtggattt cttcctgctg ctcatgcttg caggagcagg
     1081 tgatgagctt cagggcatta aacgaggcat aatggaaatg gccgacctga tagcaataac
     1141 taaagccgat ggagcaaaca taagcaggtc aaaattggcg caggctgaat ttcaaaatgc
     1201 cctacaccta tttcccatgc ccgaatcggg ttgggttcca aaagtggtta cctgctcggc
     1261 tctgaataaa gttggaattg acgaaatttg gcgtgagatg gtagcttatg ccgatttcac
     1321 caggaaaaac ggctactttg ttagccgtag aaaccgacag gcaaagtact ggatgcatga
     1381 gactataaac gaagcgctta aaaacaagtt ctacgccgac aggttaatac atgaacaagt
     1441 aagagaatta gaaaaggagg tggttgccaa caggttaagc ccatttgccg ccgcacaact
     1501 ccttcttgac cggtatttca gccttaagta gctactctgg caagcgggtc aacttgagct
     1561 gctcaaacga tgaggaatgg ataggattat tgcaaagttg attgacattg gctatccgta
     1621 atagagtgag taaaaaaatt gcacaaccag taaaacaaac tatgcgcaaa tggataagaa
     1681 acagtttctc agcaccatac taaaccgaaa gagtgttaga agttttacag gaaaaccgct
     1741 cgggcaggat gatatggagc ttcttttacg agccggtatg gctgctccct ctgcacataa
     1801 cctgcaaccc tggaagttta tagcaatagt tgacaggcag atattgaact cccttgccga
     1861 aaaacttccc tacgccaaaa tgctttttga agcccctgcc gctatagttg tatgtggcga
     1921 tttaaccaaa acaggaagca gtcccaaaga ttactgggtg caggattgtt ctgcggcaag
     1981 cgaaaacatt cttcttgcag ctgaagcgct aggcttgggt gctgtgtgga caggtgttta
     2041 ccctcgtgca gaaagggttg aaaccgttca gggaattctt aaccttcctt ccgaaattgt
     2101 tccacttaat gttatccccg tcggaacccc aaaaggagaa caccaggcta aggataagtt
     2161 taaatcggaa aatattcagt ggaatcgctg gccctaaagg ctcccaatca gggtttcctt
     2221 tgaaagagct ttgtactttt cgttaaagtt ccggtaaacc tctgctatat gcttgtagtc
     2281 gtcgctgttc ggctcaattt ttagtccatg gttgtccagc ttatcttcta tgtaggcaag
     2341 tgttaggtga tcaatatgcc tggcaggctg gtaggcgtag tttttggcta tgggcgttac
     2401 cacttcgttc agaaagccgc actccaccat ttgggtgaga agattcctca tcagcctctg
     2461 aggaattttc aactgctttg aaaggtccag gcttgtcaac ggctcttccc cattgttaaa
     2521 tcttttgatg atggtattca gtattaaaat ggaaagaact ttcctgttga agtggcttat
     2581 ctggtcggtt tccttttcaa attcgtacat ctcgacattc tggtaggcaa aagaaacctc
     2641 ggcaccaaaa agtacaatta gccagctggt atgcacccat accaggaaca ggggtagtgc
     2701 agcaaaactc ccgtaaatgg cattatactt tgatactcct atctgtaggt aaacataagc
     2761 ccattgcaca ataagaaacg ctgtgcctga aagtatgccg gcaattatcc ccgaaacaat
     2821 cgagaccttt gtgttgggaa gcgtaataaa caaaaagctg aaaagtatcc aaatcaacag
     2881 gtagggtaca agcttaaacc ccgaaagtag taccgggctt ataatgtgta tgatttggct
     2941 tttttcggct attgtgctca cctgggtaga aaggtaaatt tggattccgc tggcagcaag
     3001 aagcagaatg ggcgctataa ccataatcga aaggtaatcg gtaaattttc gcacgtaggt
     3061 tcggggtttt tctatctgcc agatgtcgtt aaaggcgttc tcgatgctcg atagcaccat
     3121 caccactgac cagaaaagta acaccactcc aatacccgca ataattccac cgcctgtgcg
     3181 ctcaagcagc gaagacgaaa aggtaatgag cttgttcata acctcctcct gtccctcaag
     3241 gctgcttcta atttgctgct ccagaaactt gtcaagccca aaacccttgg caattccaaa
     3301 caccattgca aaaatgggga ctatggacat aagggtgtag taggtaaggg ccgacgcttt
     3361 aacgctaacc tttttctcgt tgaaaccacg tagcgccagc agccccactc gaaggtactt
     3421 gtagaaggcc ttaagcttag gcgaaagctc atcaacctgt aaaacccaaa tatcatgggt
     3481 gatgaatgag tatatttgct caaacttctt tttaagccgc tggattctac tttccttttt
     3541 ctctttcttc ggttttgcag tttttaccat aaccttacaa aataaatttt ataaaggcgc
     3601 ctagctccag gagcaggtgt taaggtactc acgattttta ctgccaaata tttcggctcg
     3661 aattattaca tcaaagtctc ataatcatcc aattgctaag gcaaacactt ttcctggcat
     3721 acttctaaca accccagcaa attcaagatt cagcagcagc gataaaacct tagaaacctg
     3781 caaacctgtg tccatagcaa ttacatcaac cgtttcctca cctttttcct ttagaaagtt
     3841 aagaattttt tgctcatctg cagaaaactt atcaaagtta atagaatact ggcgtgatga
     3901 atttaccttt gaggtggtcc agcccatcag aaattctaaa tcgtcggcgg actcaaccag
     3961 tgctgcctta ttttgcttaa tcagctgatt acatccctgc gaatataccg agtttacatt
     4021 accaggcact gcaaaaacat cacggttata ggagttggca atatcggcag taaccagcgc
     4081 accgccctcc agcgcgcttt caaccacaag tgtggcatcg gccatgccag caataatccg
     4141 attccgacgt aaaaagttat tcctgtcgaa taccgcatcg gaggtgaaat cgctaaccag
     4201 cgctccctgg tttagtatag ccttggccac gcttcggtgc gattgcggat aaatggtgtt
     4261 caggccatgc cccagcaccg caacagtagg caggttgcgc tttagtgccg ccctgtgagc
     4321 ggtaacgtca attccataag ccaacccact tataattatt ggcctatacc ccttttctgc
     4381 caatcccgag actaaatcct cacacatcgc ctttccatat tctgtagccc gccgggttcc
     4441 tacaatgctt agcactttat ctgtgttaaa atcaatgtta atatttgatt ttacgtaaag
     4501 aacaacagga ccatcctcac attgctttag ccggtaaggg tatgccgcat cagtaaaata
     4561 aagtactttg atgccgaatt tttcaataaa cagtagctcg ttttcagctc gcgatagaac
     4621 tgtttgggct acaatttcgg cggcagtggc ctcgcctatt cccggtattt taagcaggct
     4681 gccttttttc tgcttaaaaa cggcttcagc acttccgcag tatgcaatga gttttttggc
     4741 gctaatactt ccaacttttg gtattagttc gatgccgatt ttatatacca gttcgggatc
     4801 ggcgctcatt tacctttcac atagctgtta agcgcagcaa ccagctgctc aagttgtttt
     4861 ccggtaagcg aggaaattga aatagccgga tacctagaaa ctttatttcc gttccggaca
     4921 aaaatgctaa gttcgcgacg aaggaacttt ttctcgatgg tgaagcggta aaactcgctc
     4981 ttgtcaacct cgatagaaat tttgggtgct ttaaatgggc gaagcagaaa atacctgatg
     5041 atgatttttt tcccctcatc gttgtacgaa aagtagctgg ctcgcctcag gtagaaatag
     5101 cccgagaaaa ggatgtagag tagcagaatg atggcaattg cccaatcccg gtctatccct
     5161 gttgtcctta gtaaaaaatc gctaaggccg gtggctatca atagaacaat taaaataatg
     5221 gctatgatgg ccattatgag tttggccgaa tggataccag ttacggtgtc gtcgttatca
     5281 aatttcatcc gttagcctta tttttcaaat tttatccgaa aaattaatat acatacggaa
     5341 ctcaaccaag ttgttggcta tgtaaaaata gaataatcat ccaacatgcc caacatttat
     5401 atcattattt aataatgcca acgcctcggt ttgtgagatt ttagttttta tggattcttt
     5461 caggtttctc cttccaccaa tttttccctt cgctttacct ctttccttaa aaaatgacgc
     5521 tcttccctca ggtggctggc tgagtaatgc tgaattatgc tgctaacgat tcccttctct
     5581 actatatttt ttggcccata gggaattgtt ttaacaacat tgtagcctga aagggcaagg
     5641 ttaaagctta aatgatcggc taggccaagg tgatgggcaa aaaatacttg agggtcgcct
     5701 ggactaaccc cgaggttttc cattaaagat agaatgtaga gaatggtgtc atggttatgg
     5761 gtaccaaata taaatgaagc agaggcaagg ttgtttagta gaaatttgat tgcctcgttt
     5821 aagctatggt tgatgtcatt ttgagtattt tgaaacaata tcttctcaga ttcgcccacc
     5881 tcccggtgtg ttagtgtgcc tctaagaagt tttgccccaa gtatgtaacc attcttggac
     5941 gcaatccgaa gctgttcaac tagcctttca aacgtctcca ccatgtcaaa cctaaacgtt
     6001 tgatacacgg ttgccctgtc tccgttatgg agcatcatca tttcgctggc aatactatca
     6061 atagcgggtt gataatcaac atgttcggcg tcaataataa tggattttcc ggaactgaaa
     6121 gcttcgttgc aaagtagcct taccctattg gcgaattctg tgtaaatacc tttgtctgca
     6181 tggtttagca tggcgttttg agataccagc cgtagtatcc tgggttcaac taaagtagaa
     6241 agtttaacca gcgtaaaggc gtggttagga aaatccctgg caattcttat agcccttagg
     6301 tactcatcaa aaaccaactg cttatcattg cattcaacaa ggggcgctgg tgaagagtaa
     6361 actccgtagc cccatagttc gtctccaacc ctttcgcagg tttccagcgt taagcctccg
     6421 gaaaaaaagt cgaatattct atttttgagc aggaagttta cgggaggaat tgatgcccac
     6481 gaattagcct tgccagaacg attcattcca cccaattttg atagcagggt gaataacctg
     6541 catgctcttt tcaactcttt gtttgacaag tactggaaag cattaccggt gttatcaagc
     6601 gctatcatct gactgcaata tttaactcat aaagttaaaa caattgtggg aatgtgtgaa
     6661 aaacagaaaa aacacctata agaaaaagtg ttgaaaatgg tttgccgctc cttggcagaa
     6721 aagaaacgtc taaatggcca tggatacaat aaaagcgccc gatgttaatt cgggcgcttt
     6781 attactacta gctattagct aaaactactt ttccctattc caacttacag ctgattgtaa
     6841 aagcttgcca tcacttaccg ccagatcctc agcatcttga tcgcgttcaa aaattggatc
     6901 aggtgcgggt tgggtacatt taaaaatacc cacataggtt tttttggcca agtcattttc
     6961 gtaggtgttg aatgtaaccc agtagtaaac aggaattcca tcaacctcta atactgcatc
     7021 gtttgcgtac ttcaacttta gcaaaatagt cagaccttcc tggagccttt gccacgttaa
     7081 cgcaattttt tcctcatcgg tggcaagttc ggcaaatccc ggcacgttgt actggcttct
     7141 tgtagaaagc ctaaggtcaa aatttttgta gtatgcgctg gctccaaagt aaaactcgtc
     7201 gttaccatag ctgcttccgt actggcgtcc ataagtaagg taaacataat caactaacag
     7261 ctgcaggtcg gaggcactag gagtcaaatg tacggttgga tcaaaaatcc aatgctctcc
     7321 agtgtggatg aactgtcccg actgctgcac aataccattg tacaccatcc aactccctgc
     7381 actattctgg tatttaccgg ccactttggt attggcgttc ggatagttgt aaaccattat
     7441 gcaggtatcc ccgtcctgtg cataggggta tttttgggaa aggtaggttg gaatataccg
     7501 gctagcggga ttagtatttg tgaaggcgtt ccctgtaagc cccattaggc tgtattcgaa
     7561 tggttgaacg caaagcaccc cttttgattt ttcccacttg gagcctgtaa acctatagta
     7621 atcattgtag tttttgaaaa ggtcggctgt aacacttttc cctttagcca acaccttggc
     7681 tgtaataacc acatcatcta cctcccatgt tctacttgcc gatgtggttg aagtgtacac
     7741 aaatgcaatg taaattttgg ctttaggaag gggaacattt cccgaaggac aaaatacaaa
     7801 gttgcctgtt cccaggtcaa aactcaggtt tgtccatgag gccgcatttg gatcgccgct
     7861 accgctgtag ttggtcgaat acttcagcag taaatcgttt ccatcgtact tccatgctgt
     7921 agtaaagttg agcgttgcgc tttgcacgcc cgagaggtca attgccggaa tgataagcca
     7981 atcctcattt tcgttgttta ctccaccgga gtaaccagat attttggcaa agtagttccc
     8041 gctatatgct gaagcagccc acttttgcgc ccctttgacg ctaacagtgt caaacttatc
     8101 caggttgttc tcgttaaaat tttccttaaa aagggtatca gtgcgtgttg ttgtactaga
     8161 tggctcaaaa tcagacgatt tataggaaac caggcattgc tggttagctg tgggcgacga
     8221 aattgcagct gtcagaatct gtggaatgaa ttcctcggca ggcatggatg gtatgaaata
     8281 accggtattt gcaacagcac ctcctaccga ttgatagttg tcggaggtaa gggtgtaaag
     8341 tgttgggctg ctgtaggcag taaggtaacc ggtgttttca agattaacgt tgtaggtcac
     8401 atttgcagta gagcctaggt ttagcgcaac aaatctgttg gcaagcactt tcccgacata
     8461 gtttttggct gtaaaccttt ggttgaatgc ctgctggtcc ttaatcagct gtgccgatgc
     8521 gctgtcggca tgattttggg catcagccaa agcgtacttg cttgcattag agtaatcggt
     8581 tgcatcaaga gtataggtga tgttttcctt atagggtttc ctggcttcgt caagattgtt
     8641 gtaggtgtcc tccatggggt tacatgccca caaaaggccc aacagcatgg taagattgag
     8701 aatccttttc atcggtgtta tcatttattg tttatttaaa ttcgtttggt taaattatta
     8761 cggttatgca acggcttgca aatcttcaac atatcaactc aactcctaaa atttgcgcgt
     8821 gcactatcca tcgtgttaaa agttgattct aagtccggca gaccacgttc taccaaagcc
     8881 atagaaaaca tacgatgaaa actcatcatg atatgttcca tccgttgcat cggcaatata
     8941 ctcggtgtcc agcaagtttg acacgttgcc atagatggta gcattgaagg atcccatttt
     9001 gaaacggtag ttggcgtcta tatcaagaag gtagtagtcg ggcatttccc atgcatccac
     9061 cttgctttca atagtcgttc tggcagttgg atcaaaatcg gcatagtttt taccaaacca
     9121 gttaaagtca acgcccacct ttagctttgg cagcacctca tagtcaaccc caacagctgc
     9181 tgttatttgg gcagagttcc ccacatgaac atcagcaatg tatgcgttat atgtaccggc
     9241 taccgattgg tcttcgttgt aaagggtaaa gttaacatca tctgtccaac ggtaatctcc
     9301 ataggagaac attcccttaa cattcagggt attaattggt ttgtagtagc attccaactc
     9361 gactccctga tgaagcgcat taattcccag aatgttggcg gtttggccat tcaatgatct
     9421 tacaagccct ttatccaacc agctggtacg gaacacgttc agcttggcat tgaaatttcg
     9481 taagttaaac ccatagccta cctcggcagt aataatcttt tcgtacttaa cgcccttgtt
     9541 gaaatcgttc gtattattca ggaaggagtt ggaaaaaatt ggggcttttt taatgtaccc
     9601 accgttaata aacacatttt gactttcact tatcttgtag ttaaaacctc ctttaatatt
     9661 ccagggcaga aagtcgtgcc atggggattt ttgatttcca ggagcatact ggaaaaaatc
     9721 aaccctacgg tagttgttgc tagcagcagc aacagaaagg aatcccgagt agctattctt
     9781 tatatactct gcctgtgcat aaagtcctaa ccacagcacc tgtcccctat agttatagtt
     9841 aacataatcg cccttatgaa gcggctgtga tgcaggacga tttatgttct tattatcaag
     9901 gtagtaaacc ccaccaagca aatcgtcaat tttatatgca tgtacaccgc tgtagtaccg
     9961 tccgtcaaaa ccggcggtaa tcttaaaatc tgaaatagat gtggtaaaag ttgaaagcac
    10021 tccataccac tgatggtcat taattgcatt ggctattatg gcttttgaac cggttgttga
    10081 agcggcattt tgtgctataa cccagtcaaa atcaaaatac ccctcgggag tgagcgcaga
    10141 gttatcataa ggtgttccgc tggggtagtt taagctcaac caggtagaat tcggaccgta
    10201 gactcttctt ccgcctccgg ttgctattga ggcataagcc tgggtagata gcatggcgtt
    10261 atcactaatt ttccagtagt ggtttaatga aatttgcggt ttgtggtagt agttgtaggc
    10321 atacccaatg ttataaatat cgccattgcg gtacccgaaa tccgaattcc acttagcctc
    10381 catggggtga tcgcggaatt cctgaatagt atgcttgttg ctgcgctggt tatgccactg
    10441 aggtgctcca aatgcattga aaactatgct atgcttactg ttaatttttt tggaaacatt
    10501 aacaaaatat gaccaacctt caaaattagt agcctgaaca tagccattgc cgtatgtatg
    10561 tccccccgaa agagtcatag cccaaccgtt atccaacatg ccggtggata ccgtaaaagt
    10621 ttgcttcata tacccatcgt tcccaatgcc cgcatatacc gacccgccct tttcagcgtc
    10681 tgttgacctg gtgataatgt tgattgttcc tccaaccgag gaaagggcaa gtttggatgc
    10741 acccagacca cgttgaacct gctggttgga agtaacatcc gaaagaccag cccagttact
    10801 ccagtacacc ttgccatcct ccatgccatt taccggaatt ccattaatta gcacgcccac
    10861 gttgttggag tcaaaacccc taacgtaaac gcgggaatcg ccaaaacctc caccgctttt
    10921 ggttgcataa attgaaggcg ttgatttaag aatttcggga aattctttat tcccaagctt
    10981 ttcggcaatg attttgggct caatggttga aacggcaacc ggtgtggttc ggtcacgaac
    11041 aaacgacgaa accaccagca cctcttccag ccctattgag ctggccgtaa gctgtatttg
    11101 ccctaaatcc ttttctgttc ccgagggaat ggttacctca attgttttat cggaataacc
    11161 aacaaatgta accttgatgg ttcctttctc tacaggtact tttaatacaa aagaaccatc
    11221 caacgaggtt aacgttccaa ttgttgtgtt ttcgactgct actgcagcgc caactaacgc
    11281 ttcgccactg ttgctgtcaa caacaacacc tttcacaata ccctgtgaaa aggctactcc
    11341 tgctgttcct actatacaaa cggtaagtag gattttttta ataactttca tcatactgtt
    11401 atgaatttgg ttagggttaa gtgtagctat tgattgtgat ctacaatttt gtttgcgtat
    11461 tgaagttgtg aaaaataacc tgatggctta agggtgaata ttaataaaat actaacctcc
    11521 acttctcgct tgcaggaaac cgacccccgt ttaaccgaaa gcgttgttcc tttagtgttt
    11581 aagctgaatt tttgtgcacc aaattacaaa catgaatttc aaagtgtatt atacggctaa
    11641 cctttcttta ccatcaacta aaatttggaa taaactttaa cagtaattcc aatatggttg
    11701 atttattttc taggtcaagc ccttagaaca tggcgttttg aaaacattta aactatgagg
    11761 gtaaatgcta acacgcctag attcaaataa ttaaaacaac atatatgagt ctattagtac
    11821 ttaatgcaca atgcaaaaat ttaagaggtt acgtttaact cataaggtta aagcaatcat
    11881 tggtgtgagc atataggata agccggataa atgattgggt aaaatgataa aaaaatccgt
    11941 ccagaaaacc cggacggatt atttattaag taaacatggc ttttagaaaa tcaaccttat
    12001 accaagtaga gcaccccatg tactggttgt gctgatgttt ttgctgaggg ttgttttagt
    12061 ctcaaattct tcaattgtgt tggcattaag cctgaatgta ggagcggtag ttgcggttgg
    12121 cagggcaacc ctggttaaag gacggacatt gtcgtagcta gccagcgggt tgtaggtata
    12181 ggcgccccac ttgtcgttta gcatgttacc aacgttgagc aagtcaaggc ttatttgcag
    12241 tgtatatctc ctgctggttc caaagttgga gaaaatatcc tgcagcacct ttacatcgaa
    12301 cctgtgaagc catggttgaa catcaccaaa acgttctgca tacttgcctt tacgatcttt
    12361 caggtaatca ttaccgttaa cgtaagccca aaaagcatca gcctgttgtt gtgcagtcat
    12421 ttcaacacct cctgttgggg tatagggaac aaacatggcc agagcttcgt ccttgtcctt
    12481 tggaacatag atcaggtcgg aggatatgcc atcgttattt aagtcgttgg agtaggtata
    12541 ggaccagcgt ccttgctgaa atcctctata ggctaacgaa atagtagtgg caaacattcc
    12601 accatactca attctgtagg aggcatggcc aatcagctgg tgtggagtgg caaagtttga
    12661 gtagcttagg ccaggattgt taagacttcc cacagcagta tttgaggagt aggcggagta
    12721 ggcggcagaa cctgggttag aggagatatc cttggccatg gtgtaggtgt aggcaaacat
    12781 tccggaaaaa cccttgctga agtttttggt tagctgggca gtgaatgagt actggtaacc
    12841 tttgtcggta ttagaaagaa caacagcatt ggctattgtg gaaaccacct ttctatcgtt
    12901 tgctgttgcc caaaacggcc tattatctgg accaaccata ttgccagttg gattggcttc
    12961 gttgatgtta atctgctgca cggcattaat atctttccca aataatgcct caccggtaaa
    13021 cacaaagtta tagggtagct caacatcaac cgccaggttg gaacgccaaa cctggggcat
    13081 cttaaagtct tttgaaactt cagccaatcc ggcattatta ggcaatgttc cgggtgcttg
    13141 agggaaaagc ggaggattgc ttgctatgaa ttccttgaag ttgggattaa actgcaagtc
    13201 cactaaattc gcattgccag ggccccatcc aatctcgggt gattgaatca tacccgagtt
    13261 tgttggctgg ttggtgaacc aaacgaaggg taaaagccct gtaaaaatac cggtacctcc
    13321 tcgaacctgc aacgagcgat cgcctttaac atcccagtta aatcctaaac gaggggagaa
    13381 aagcagctgc ggatcgggcc aactacccac atcaatcttt tgactatttg cgaaggtaag
    13441 tgcagaaatt gcagggttat ccttcatgtc gtcgaagtag aagggaagct ccaaacgaat
    13501 gccataggta actttcaggt tggtattcac cttccactca tcctgagcat aaaaagctcc
    13561 cattccaaaa gtaagctcag ctccgggagc gtcttcacca ttatacccgt aggttactcc
    13621 aaaccctatg ggatcagcac catttatgaa atcatcaacc gatgcatacc ggtagtaggt
    13681 tgtcccctcc cggatgtatg aattcttaaa gtaaagccgg tcaaaggaaa ccccaagggt
    13741 gagagtgtgt ttttttaggt taattgtggt gttgttgagc aaacttaacg tattgttgat
    13801 cacgtcgttg ttatatgaaa acaactcgta tccaaaggac atatactgat cgccgtcctc
    13861 ccatatatct acaaagggga acagctttga tggagatgtt ctggaatcct gaatgtgggt
    13921 gtagctacct aaaaacttgt ttgaaatatt cgttaaaaag gtgctgttta gctcaccggt
    13981 aatcgaacga acagtgttct caaaaccgta gaacgagtta gagaaagcca gggattggct
    14041 actaattctt cctgaatttc tagcgttgtt gggaggtcca ctgttaaagt tggtttcaac
    14101 gtccgaagta cccactacgt cgttatagcg aatggtaaac ttatgatttt tgctgatgtt
    14161 ccagtcaaca cgcgctaaaa ttttggtgtt tttgttctgg aagggatcaa agtttttata
    14221 cttacccggg tcatagttat atgtgttgat tagatgatcc cttaccctaa tcatatcggc
    14281 ttcggttgta cgcgaaatca tctgatcggg gttggcaacc ccatcggtac tgggtttcca
    14341 tgtaacccca ggaatatctt cattttcaat ttctccattt ataaaaaaga acaacttatc
    14401 ctttataatt ggtccgccaa aacttaaccc aaaattttgg ctgttcctgt cgtttgcccc
    14461 ttctacgtcg tttccgtcaa ccttatcgcc ggtaaatgat tttgggcgaa tgtaggagta
    14521 agccgaaccc ttaaaggagt tgtctccact gcgggtaacc gcatttatgc tggcaccggt
    14581 aaactgggaa actctaacat caaaaggtgc aatattcaca gaaacctggt caatggcgtc
    14641 caaggatatt ggctgtgcat ttcctcccgg aagggggttg ctgcttaaac caaaattgtt
    14701 gttgaaggct gcaccatcaa cagtaatcgt gttaaaacga ccatctcttc caacaaaaga
    14761 gttaccctgg gcttggggtg ttaactttga aaagtcggtg atgctgcggg aaattgaagg
    14821 cagtgtgttt atgtcacccc tgctgatgtt ggtttgtgca ccggttcttt ccgaactcaa
    14881 aataggattc cccattcctg ccgaaacgac aacctcctct agggatgttg ttgattcttt
    14941 taactttaca ttttgcaggt acgattcacc caggttaagg ttaatgtccg tataaactcc
    15001 cgtagagtac cctacaaacg aaatgcttaa cttgtagggt ccaccaacac gcaggttggt
    15061 taaacgatag ttaccagcat tgtcagtagt ggtaacatac tgcgttcccg aaggctcatg
    15121 gatggccaca acagtggcaa atggcaatgc cattcctttt tcatctacta ccttaccgtt
    15181 tatttccgaa gttgttacct gggcgtttgc ccataagcca acaacggtaa acaacatggc
    15241 gataaaaaac ctgatttttt tcattaaatt aaaatttggt tagtaatggt ttaattattg
    15301 gcaaataaat taacacataa ggtgcatgta aaacaaaact ttatataata ggtttaactt
    15361 ttgctactgc attcgaaata tctggactta cccaaaaatc actttccaac atcatactac
    15421 ttgaatatgt ttcggatttg cagctggtaa tacggatact tttttgtcat gccaaagatg
    15481 agtcaaaata atctaacggc tgatagctat atattaaaaa atgattaagt ctaaacggtc
    15541 aaagggttta aatctctttt ttgaacgaaa ttaaggtttt gcccttcttc ttgcctgaaa
    15601 ataactgcac taagttataa aaatatgcct tatagtgttt tcaatgttaa acttatctca
    15661 gcgtaattct agagttttgg aataaaaaca agccatttca aaataagtgt ttgcattatt
    15721 tccatataaa tttattataa taggattttt tgcttggtgt cttgaaatat gctaacagta
    15781 gccccagcta cctttttgcc acagcgctga tagcttctgc ctgtcgccct cagccacctc
    15841 ccataggtcg aagtcttcct gcaaccctta tagttccagc acaacccctt ccctgtcatt
    15901 ctgcaggttc tcatcagccc accggtagca ttgcagaggt aaagtttcga ttcctcacca
    15961 gaaggcttcc tgttgtcaac tgaaagggat taaacccaaa cctccgacat gttgctagcg
    16021 acggagctcg gctaccacct cctcgttggg tattcatcca cagctatgcg cctctgagaa
    16081 tgctacacag caattgttga taaatttggc aaatctacta gtagcgttaa cactcaacgc
    16141 ccatctagtg gttctaagta acgggattaa actaccggct ttgagatcat atcctaaatt
    16201 tgaagatacc caggcgactg accctagaat tttattccca ttaccagaat gtcgtcaacc
    16261 tgctcgtagg tacccttcca atcggaatgg ctggcagcca aaatgttttc ctgttgggtc
    16321 atggaaagcc cagcaatgga ttttaaaagg ttgataagat tttgcctctt gtacttttgg
    16381 tagttggggc cgcctagctg atccgaaaag ccatctgtaa aaaggtatag catatcgtgt
    16441 ttgcgtagct ctacctggtg gttttcaaaa ggcatttcct ttggatagta tccaatgggg
    16501 tttcgtgtag gcctgaattc cagcacctcg ccattcctga ttattgttaa cggcatgttg
    16561 gctcctgaat actcaagata tgttgaggct gggttgaacc tgcaaatagc catatccata
    16621 ccatccttgg tttcaacccg tttacccgac tgtttgaggg agttttttac gtttaccctt
    16681 aactcctcaa gaatttggct tggagatatc gaactcttcg gggtaacaat atcgtccaga
    16741 agggttatgc ccaaaatgct cataaacgcc ccgggtacac catgccccgt gcaatcagca
    16801 aatgccagaa tacctgcgtt ttcttgaagc ttaaaccagt aaaaatcgcc gctgacaata
    16861 tctcggggaa gataaaaaat aaaaaattca gggaaaaatg ctgaaagttg ctccctggat
    16921 gggagaatgg tcgactggat gtgccgggca tactcaatgc tatcggtaat cagcctgttc
    16981 tgacggatta ttctatcgcg ctggctaacc acctcatctc gttgtgtttg gatttcttcc
    17041 ttttgctgcg aaatcaagct attactttgt tccagaattt cgttgtcgcg ttttttcctg
    17101 cggtagctcg ataccagcac cccaactaac ccaataagaa gaacagaaag cagaagttca
    17161 gacaagagaa taatatccct cctttttacc ctttcctttt gaagttcgat ttccttttcc
    17221 ttttgaaccg tttcatactt ggtgaggagc tctgccaggt ttttctggtt atccctggcc
    17281 agaatcgtgt ccttccacat ggtgtaaatt tcgtagtact ttagggcttc ttcaaaatta
    17341 tttaactttt tattcaggct ataaatttgt ccggccagcg tcattttaag gtttggctgg
    17401 ttaagagagt caatatagct caaggtttta gcgtagtaac tttttgccat tgcactttta
    17461 ttcaagctat cgtaagcaca gccgatgctc atcagacaga gggctgcatc gttttttgcc
    17521 ttgacctttt caaaagcatc gaaggccttt tcctggtagg ctattgactt ttcgtagttc
    17581 ttgagggcaa actctacttc tccaatatta gcaaggcatt gggccgattt atagtagttt
    17641 ccctggttga tttcaaattt ttgagccctg ctgaagtatt cgagtgcctt tttgtaatcc
    17701 tttttattct tataaataac tccaatgttc atgagcgctg ttgcctcgta atcggcccat
    17761 cccagtgtat ggaacatgct ttgcgccttt tggtagtact ccaacgactg gtccagctta
    17821 tcaaggctta agtacaggtt tcccagcacg ccgtaaacgg ttgcaactcc cttttggttg
    17881 tttcgttttt cgaagatatc cagcgcctta aggaaacttt caaccgccat gctgtatttt
    17941 tgctcgaaca tgtaggtgtt gccaatattt ccataggcag aggcaataag gcttgaatcg
    18001 tgagcgctta tggccatctc aagcacccgg ctaaagcgct cccttgcctt ttcaaaactt
    18061 gagttcacgt taaaaataaa gccataagtg ttttcgcacc gaatcattag ggctgtgtcg
    18121 gaaagatccg tggctagctg gtgggcaatt tggttgaacg ccatagctga gtcagtatcg
    18181 atgtttatat atctttgagc caaatctatg gctgtttcgg cctttaccga tgtgttcggt
    18241 gcttcattca gctgttttag caggctatca atgtccgacc tttggccaaa agtcgtaaac
    18301 ataggtagaa tagcgagtat ggctgaaatt aatttcctca taatcagatt tttcaataaa
    18361 tttatgcctt tttacctata tgatgtaaaa attcaccata aatttttaca tcctaactca
    18421 gaataaatct tctactgcag attgctgctt tactgccatt ccaagccagg aaaagctatt
    18481 ctaaacaaaa cctacttgta gaatgctcct caggaacaat gccaaactaa aaaatcggca
    18541 gcaatcagag ctgcaataaa actttgatcc tacaccatta aattttccat tggcggctaa
    18601 atcgttttag atatttacag tatatttgtg gaaaaggctc aaacacggtg aagaaggtaa
    18661 cccttaacga cattgcaaaa aaggttggcg tatcgaaaac tctggtatcc cttgtggtaa
    18721 acgggaaggc taggggttac ggaatttctg gacaaacctg cgacaaagtt atggcagcag
    18781 ttagggcgta tggatacaat cccaaccaaa cagcacgaag cctgcgtacc gggatgagca
    18841 acactttggg cctaattgtt tcggatatct ccaacaactt ttatgcctct ttggcaagac
    18901 aactggagga tttggcctgg gagcttggct acaacatgat aataggcagc accgacgaaa
    18961 gcgtgggcaa agagcttaaa ctccttgaaa tttttcgtaa ccggcaggtg gatggaatta
    19021 ttctctcctc ttcgcagcaa aacccaaccg agctggaaac tttggtaaaa tccggattcc
    19081 cgctggtact cattgacagg tactttccta ctgtacctat accgtcggct gttgttaaca
    19141 acaaggttgg tggagaatta ctggcacagc acctgctaaa tcagggtttt cgcaacccag
    19201 tggtactctc cctaacccca agctacattt catccgttgc cgaacgggtt gagggtttcg
    19261 taagtgtttt tgagaatgca gccattcata ctgaagtgca caatattccc ttcctctgcc
    19321 tggtcgacat ggttgaggat tttttcacga ctcgtctcca tgataagaat atgcccgatg
    19381 cagtttttgc cgttaacaat aacctggcaa cagcgactct ccgagcctgt catgttcaca
    19441 acattgttat tcctaaggat attgcgctgg tttgttttga cgaacttcct tactttgatt
    19501 tctccaaacc atccatcacc tctataaggc aacctgttgc cgagctgtgc agcgtagcat
    19561 tttcgttgtt gctagggcaa attacaaata acggggaaac aaaacccggc catgtagaaa
    19621 tgatgcccgt tgagcttttg gtgcgggaat cgtcaactca aatttctgat aataatggat
    19681 ggcggtcacc ttaatgcaaa ggacacaaac ctatgattgt aaaccaactt aaaccatgat
    19741 acaaccagcc cataaaatga ctataattca ctttatatca ggtgtttcat tgctttttca
    19801 taaatcgcat ttggcaatgt cgcagtacaa atcaccagcc gatttcatat taaggtgtgc
    19861 taaatcagta attttaaaca ctcatgtaag gcagactgca caaacacgca tgaataagcg
    19921 aacgttactt gcaagttcgg caaagtcaaa atatgggggt tccattgctc taccccgata
    19981 acgaaaagct gtgggctagc gtgcttgtaa ttaaaaaacc taaaaaactt tttcagttta
    20041 aaccttgagt tatgaataga aaaacattta caattctttt ggccttccta tgtatgggat
    20101 ttggcgatgc cgttggacca tttgtgggtc tggcaaaaga gcagttcgag ctttcaaact
    20161 ttgaagcgca gctaattgcc ttcatgggat tcatcatgtt tggctttttg tctgtaccaa
    20221 tgggaatatt ccaagatcga cgggggaaga aagttgtgct gatgctgggt ctggcagttg
    20281 ccctagccgg gttaaccatt ccgttatttg gactgggcag ctatgcgttg ttcctcctga
    20341 caattctgct tctgggcgca ggtgctgcaa ttctgcaggt agcaggaaat cccatcatgc
    20401 gtgatgtttc tgaacccggg atgtactccc gaaaccttag cttcggccag ttcattaaag
    20461 ctattggatc gctgtcgggt tccattattc catttgccgc cgcaaaatgg atgggggccg
    20521 attggcaagt tttatttcct atttacgtgt cagccctgct gttaacgttg gtcgccgtgg
    20581 gattcacgaa ggtagaggag gagaagtccg ataagaaacc tgcaacactc aacagttgct
    20641 tttcgctatt aaaaaatcgc tttgtccttt taatggtgct gggcattttt atctacgtcg
    20701 gaattgaaat ttgcctcagc tcgggtgttc ctatcctgct tagcacccat ttcgggatcg
    20761 acctaaaaac atgggggttg cttggtaatg cctttttctt catcgccatt cttattggta
    20821 ggcttcttgg ttccattatt ctcagctgga caaaaccggc agcatttttc agggtttcat
    20881 gtctcattgc gctaatgggt atcctgctca ttttttccaa caaccagatt accacattgg
    20941 taggaatatt cactaccggt cttgggtttg caaacatttt ccctttgatt ttttcaatta
    21001 cggtcgatgc catgccagag cgggctaacg aaatatcggg tttaatggtt accgcaatcg
    21061 cgggtggcgc atttattccc ccagttatgg gcttggtggc cgatatgtca agcgttagaa
    21121 caggcttttt ggttcctttg ctgggtcttg gatttattat tgttctatca tttttaaaac
    21181 ctcaaccctc cagacaatga gctacttcga cgatagcaga attgtgctaa cccttgacgc
    21241 aggcggaacc aacttcgtat tttcggctct acaggggata aaacccattg ccgatacaat
    21301 tacactaccc agcaacggta acaacctcga aaagtgtctt cagggaatcg tggaagggtt
    21361 caccaaacta ggagctaagc taaaagggaa accttcggcc atcagctttg ccttccccgg
    21421 acctgcagac tatcccaaag gaattattgg cgatttgggg aatcttccag gattccgggg
    21481 tggcgttgcc ctgggaccaa tgctacagga gaagttcgga attcctgtct ttatcaataa
    21541 cgatggcgat ctctttgcct tcggagaggc catcgcaggt tttctgccct gggtaaacag
    21601 catgcttaaa aatgccggaa gcccaaaaaa tttctacaac ctacttggag taactattgg
    21661 cacgggttta ggaggcggtc tggtgacgaa tggtaagctt tttaccggcg ataactctgc
    21721 cggaacagaa gtttggcttc tgcgcgatgg tgtcaaccca acctgctttg ccgaggaaga
    21781 aatcagcatt agggcagtgc aacgaacata ccatgcacat acgaggaaaa atgaaagcac
    21841 cttgctttca cccaaagaaa ttgctgaaat agcctacgga aaccacgagg gcgatatggt
    21901 tgctgccaag ttagcctacc agtcgcttgg cgcctccctc ggcgatgctc tggcaaatat
    21961 cataactata accgattcga ttgtagttgt tggaggtgga ttgtcaaacg cctaccatct
    22021 ttttgcccct gccatgcttg cccagctaaa tggaagcatt tcaacagtta ccggagagca
    22081 ggtaaaccgc ttggagtcaa aggtgtttga ccttgagaat gaagggcagt gcaacctttt
    22141 tgttaagggt gaaaatcgga aaatcaaagt tcccctaagc agcaaagagg ttgactacga
    22201 tcctcttaaa cgggtaggca ttggcgtttc acgtttaggg accagccatg ccgttgctat
    22261 aggagcctat gtttatgcca ttaacatgct tgaccataac acattacata gccaggctat
    22321 ttaatgcttt aatcctccat catctaataa atttaaggcg aacgcttaat gctaatgtat
    22381 gaaagcaaat ccaaggtaat aggagtaatc cccaccccat gtttcagggt gttggtttta
    22441 ttagacaaaa aatgcaaaca ccaacaagtt tggaataaaa gtaaactagg ccataataaa
    22501 ataacaccac ttataaatac ctataaacaa tgaaaaagcc gcaactattg acagttgtaa
    22561 tgctcatcag cattgagctg ttttcacagg tgaaagaccc cgtggattac gttaatccat
    22621 ttattggtac cgattttcac ggacacacct atccgggggc aaccgttccc tttggaatgg
    22681 ttcagcttag tcccgataca aggctaaccg gttgggatgg ctgttcgggg tatcattact
    22741 ccgatagggt aatctatggg ttctctcata ctcaccttag cggtaccggg gcttctgatt
    22801 atggcgatat tctcatagcc cctgtttctg gcaaacccgt ctttgaaaac acgcggtatt
    22861 cttctccatt taaaaaggaa aatgaaatgg cggttgccgg ttactactcg gtttttctcg
    22921 acaagtacaa ggttcaggct gaacttaccg caacccgtag ggttggcttc caccgctaca
    22981 cctttaagaa tcccaataaa gcggcgctgg ttattgatct cgagcatcgc gatagggtca
    23041 tcgaatcttg gttaaaggta gtagggaaga acgagctgca tgggtttcgc cgatctagcc
    23101 actgggcaaa cgaccagcag ctatactttg ttatccgatt ttcccaaccc tttgaaaagg
    23161 ggccaaactt cgtggatgcc agcgaacagc taagcataac gaacggcgaa aatggtgaag
    23221 ttttggctga gggaaccagg cttaaggcat ggcttctgtt tcctaaactt ccaaacggta
    23281 aacttcttgt aaaggtcgga ctttcagccg tgagcgagga gggggcttta cgcaacctgg
    23341 aagccgaggt gcccggttgg gatttcgata gcgttgccgc tcaggccaaa aacgactgga
    23401 atatcgagct gtcaaaaatt aaagtggacg gcggcactcc cgaccagctg accaccttct
    23461 acacagcttt gtaccacgct atggttgtcc ccaatgtgtt ctctgatgtt gatgggagct
    23521 accgtgccat ggataaccag gtgcaccatg ccgaaggttt tactccctat accgtttttt
    23581 cgctttggga tacctacagg gcttggcacc cgctaatgac aattattgat actcgacgta
    23641 ctaccgatta tattaacact ttcctgtctg attaccgctt cggtggtcgc ttgcccgtgt
    23701 gggagctggc tgccaacgaa accgagtgca tgattgggta ccattcggtt cccgttatag
    23761 ccgacgcatg gataaaagga atcagaaatt ttgatcccga tctagccctg aaggcaatgc
    23821 tccatagcgc aaatctcaac cattttgggc taaaggagta caggcagtac ggttttattc
    23881 ctggcgatat ggagcatgag tcggtttcca aaacactgga gtatgcctat gacgactggt
    23941 gtattgctca atttgcgaaa tccattgccg atagctctac ctaccaaaag ttcattatgc
    24001 gggcacagtc atacaagaat ctcttcgacc catccactca cttcatgcga ccacggataa
    24061 acggcggttg gaaggataac tttaacccat ccgaggttga ccaaaacttc accgaggcaa
    24121 acagctggca gtacagcttc tatgtacctc atgatgtttc cggccttatt gaactccacg
    24181 gcggaaagaa aaatttcatc ggtaggttgg atgagctatt ttcaacctca tcccagctga
    24241 tggggaaaaa tcaagttgat ataaccggcc ttattgggca gtatgctcat ggaaacgaac
    24301 ccagccacca tatggcatac ctttactgct atgccgggca accgtggaaa acacaaaagc
    24361 ttgtacgcaa gattatggac gagatgtaca cctcaaaacc cgatggcctt tgtggtaacg
    24421 aggattgcgg acaaatgagc gcatggtaca ttttaagtgc agcaggattt tatccggtct
    24481 gcccgggaag tacacaactc gtcattggtt ctccgctctt cagtaaaatt acctttaacc
    24541 tggaaaacgg aaagtatttt tcggtggtag ccgagaacaa ctcgcctgaa aatatttaca
    24601 tcaactctgc ttcgcttaat ggtaaaccca ttacccgctg ctggattgac tacaacgaga
    24661 taatggcagg gggcgaactc cgtttagcta tgtcgcctac tcctaatagc ggctggggca
    24721 gcaccgatgc tgatatgcca acctcatcaa ttgatgagca ttttatagct actgctccgg
    24781 ccttcaacac ctcaactaaa acatacatgg acagcttgca gctaaccctc acaactgtcc
    24841 agccccaagc ctcaactttt tattcggtgg cagaaataaa ccaccccgaa gccattccaa
    24901 tatggacaca agggaatacg ctgaatctta agagctccaa acgggttaaa gcctactccg
    24961 ttcttccggg tggcgaatcc agcgccatag ttgagggttc ctttatccga atcgagggaa
    25021 ttaaaaatgt tgagctgaaa agcatcccta gccctcttta cgatggtggt ggaaaacttg
    25081 cgctggttga cgggcaaaga ggctctacaa atttcaggtt gggaggctgg cagggctacc
    25141 aaaatcagga ttttgaggca attgttgacc tgggccatga acgtgaactt cactatcttg
    25201 gagcagagtt ccttcaggat gcctcgccat ggattatgat gccccggcaa gttaccttct
    25261 ctgtttcgtc cgatggtact agctactcca acattggagt ggtcaacaac gacatccccg
    25321 atactttaga aaagcccata gtaaaagaac tcggagtagc tgttaatgtc gaggctcgct
    25381 acgtaaaggt atttgcaaaa tcgtatggaa aactgccatc gtcgcatgaa agtgccggcg
    25441 aatcatcctg gctatttatt gacgaaatag taatccgtta gctaaatgtt cagcttatga
    25501 acccacaaga cgcataaaca taccaacaca cattttagcc gccaacctgc tgaaccacaa
    25561 aatcatggtc atttaaggct ggtccttctc ctattttttt gtaataattt tggcttttgc
    25621 tttacttcag gaaatttttg ttgtatctta tagctgtaat ccaacaccat gtaaaatgaa
    25681 taatgatttt atctgtccaa aatgtgaggg atacctggtt gtaggaggca gcgtgattct
    25741 ttctattagc atgcaaagcg gattacgagg tcttatcctg cttagtcctg aacttggaaa
    25801 ctatgccaag gtgagtagcc ctcatgttaa aattgaaaat ggcgaaatgc tggactacta
    25861 ctgtcccatt tgccatgcca agctggctgc ttcagatgta caccctcgtc tggtaaggct
    25921 tatcatgatt gacgacaaat ccaagcggca tgagattttc ttctcgggaa ttgtggggga
    25981 acactgcacc tacaagattt cggagactgt atatgagaaa tttggcgaat catctgccac
    26041 ttacgatcag tacttcaaaa cccgtagaat ataggctgac atccatgtgt agaaaaatat
    26101 agcctagcca gggcggttgc actcgggtta tgcttcgtgg tttatctttc acgcgagacc
    26161 tcctatgggg caaatttttg atggggcatg ttgtgttggt atactaaact tccataccta
    26221 aacctattgc tattccctgt tgcgccggag ttataggggg attatcatta aaaatgaaat
    26281 atgtaaagct ggtaactagg caattccggg cttgcctatc tatccgaaga tgccctgcct
    26341 gcatttttaa ctgaaagcaa catcggccgg gttgcgccct aagcgatgta ttcagttgag
    26401 gcgctcctcc tcagtctaat ggagaaatta atacctcgaa taaatttatt atatttgtat
    26461 tttggctagt atcacgacgt tttccctgca gttttaacta gcattctgtt gcttgcaacc
    26521 acaataagca tttttacaaa atccgattcc gctcatttaa ccctttacaa aatgataaca
    26581 gtaattatac ccacactcaa cgaacaaaat acaatctcgc aggtaattga acttgtggaa
    26641 aaatctgcaa acgtatcaga aattttggta gttgatgata aatctttgga tagcacagta
    26701 aaaattgcca gggcaaaaaa cgtaaagatt tatacaagca caaaattggg taagggttct
    26761 tcaatgtatg agggaatgct actcgccaca aatggtataa ttgtatattt agacgctgac
    26821 ataactacct atcctgaaaa tattgttaat ttactaaccg acccaataat aaacaatact
    26881 gcagattttg taaaagcaac atttaatagg caagccggca gggttactga attagtggca
    26941 aaacctttat taagccttct tttccctgaa cttgctcatt tttctcaacc cttaagtgga
    27001 atgattgcag caaaaaaaag tattctggaa aaggctactt tcgaaaacga ttatggtgtc
    27061 gacgttgggc tgcttattga tatgttccag gaaaaagcac gaattgtaga agtaaatata
    27121 ggatatatag aaaataggat gcagtcgtgg gaacaacttg ctaaaatgtc taaagaagtt
    27181 tcaagggcaa tcttaaaacg tgcagaacta ataaaacact ctaatttgga gacacttggt
    27241 aattttgcta tgattagaga ccaaatggat tttgctatta aggaaagtct gcttggttta
    27301 aacaaaatgt taattttcga catggataac accattctta caaaaagttt cattttaaca
    27361 ctggctacaa attataaatt taaagaaaaa ttaattgaaa ttgtagctga aaatagtagc
    27421 ccttatgtcc gaacaaaatt aattgcacaa ttacttaaag gtttaaaaat aaacgaattg
    27481 ctggaaacat tagatggcat tcctactgtt aatgattttg aaagaattgt taaggagttt
    27541 aaacaaaaag gttatatatg cggtataata agtgatagct acgattgcat aacaaatcat
    27601 cttgtaaata aatttgattt ggacttttct atcgcaaacg aattagaatt ttcaaacggc
    27661 atggcaacag gggaagtcaa aattccttct gcgtttttac gtactaaaga tagtacatgt
    27721 aatcatgatt tttgtaaatc caacgccatg cgagaagttg cacaccgcta tcatattgat
    27781 ttacacaata taattgctgt gggtgacgat gaaaatgata tttgcatgat aaaggattgt
    27841 ggaatcggcg ttgcgttttg ttcttcaaat tcaacattga attttatagc agatagagta
    27901 atccaagaaa gaacatttgc ccctttgata gagcttctga aataaaaaat aaatataaag
    27961 ctttgctttt tatcaattta ataaagtaaa ttctaaaaag gaggataagc ttttagacat
    28021 tttgctacct atattgaaaa gatgtccttt ttcaaaatat acccactccg tttcaacata
    28081 gtccgaatcc cgatggaata gcctcaaaat ggaacatttg agttcggctg ctgataacat
    28141 gcctagccaa acccttaaaa aaatttttca catcatttaa aatggttacc tttacccccg
    28201 acttttgggg gtgcctttaa ggctgagatt acaccctttg aacctgatcc gggtaatacc
    28261 ggcgaaggga aaaagagttt taggtatcaa tctcaagttt ttttacttcc ccctttttta
    28321 aaaggctact caagagcatc aaccggggtg cacaccgcta ttgcttttgg tggcccgatg
    28381 tttaacactt aaaaaagggt aaaaaccatg aaaaaatttt ttcttttgtt gctcatgcag
    28441 cttgcagctg cttgggcttt tggtaatcca accgattttt caggctcatt aaaagttagc
    28501 ggtagggtta ccgattctga aggtaatccg ctagttggag caaccgtagc tcttgagggt
    28561 accctgctgg gaacctcaac caaccccgat ggcagttttg tccttaaccg cttgcgaccc
    28621 ggaacctatg tgctgatcgc ctcatttgtc ggtttcgaaa ccgccaggct caacctggtg
    28681 ctgcaagaaa atgtagttat caacattact cttaaggagt caacgctgat gttcgacgag
    28741 gttgtagtaa gcgctactcg ggcttccgga cgtatgccaa ttgcccaaac taccataagc
    28801 aaggagcaaa ttgacgggaa caacactggt tttgatgttc cttatctcct ggaacttcaa
    28861 ccctccgttg ttgcaacctc cgaggggggg actggcattg gcaacacggc tattcgcatt
    28921 cgaggaactg atatgtcgcg aataaacgtt acggttaaca gcgtgcccct gaacgatgcc
    28981 gaatcgcagg gcgttttttg ggtcgatttg cccgacttca ctgcttcggt agataacctt
    29041 caggtgcaac gcggggtggg tacttcaaca aacggtgccg gggcatttgg agcaactatc
    29101 aactttcaaa cggtttccct atcgcaaaca ccatttacct cattcgacat ttttgcgggc
    29161 tcctacaaca cctggaaact ctctgctcgg ttcggaacgg gtttggtaaa agaaaaattt
    29221 agctttgagg gtcgttactc gcagcttggc tccgatggat atattgatcg cgcgggctcc
    29281 gaccaccggt caatgttcat gaccgcagca tggcacggcg ctaaaagtct gattagggtt
    29341 aacatcatcc atggcgagga gcacacggga ataacatggg agggcacacc cgattacatg
    29401 ttacagatta accgccgcta caaccccgcc ggtgagtaca ccgatgaatt cggcaatacc
    29461 cgctactata aagaccagaa ggacaactac tggcaaaccc actaccagct tatttcaacc
    29521 ttctccctgg gttctaactt aacacttaac gctaccgttc atctgacgga tggaaaaggg
    29581 tactacgagg agttcaagga ggacagaaag ttttcaaaat atgcgctgcc taacctcgtt
    29641 cttggaattg acaccattcg cactagcgat atcatccagc gcaaatggat ggaaaaccag
    29701 ttctacggtg cggttgcctc ggtaaactac cgaaaaaaat ctcttgaagt tgctgtcggt
    29761 ggtggttgga accggtacga tggcgaccac tttggccgga ttctctggag tgcttataac
    29821 tacgggttac ctaaagatta cgaatggtac cgaaacaacg gcctaaaaac cgactggaat
    29881 atctttgcca aatcggtttg gcaacccggt aaaatgctta gcctttttgc cgatattcag
    29941 ctgcgcggca tacaatatga cctaaagggt ccggactcag accttctgcc ggttgaccag
    30001 gaacatagct ggcattttgt taacccaaag attggtacaa catttaggtt ttctcctgca
    30061 agcgaactct acgtttcatt tggagttgcc aaccgtgaac cgtcgcgaag cgacctcaag
    30121 gatgccctaa agggcgcaac aggcattgcc ccaaaacccg aaagacttta cgactgggag
    30181 ttgggatatg gccttaggca tcaggatttt gcgattaaca caaatattta ctacatgaag
    30241 tacagggacc agctggtgct aaccggtgag cttacagatg ttggctatgc gcttatggac
    30301 aacgtccctg atagctaccg ggccggagtt gagattcttc ttggtttaaa gccatttcaa
    30361 tggctcaacg tggaggccaa cacaactttt agccgcaaca ttattgaaca ctacactaac
    30421 tacgtcaatt taaccgataa cccaaccgat tggaatgaaa cagggttaac cgaagcagaa
    30481 tatcttggta acacaaccat ttcctactcc ccctccgtgg tgggttcagc taaaattacg
    30541 gttaccccgg ttaaagcact ttacttttca tggattggta agtatgtggg caagcagtac
    30601 atagataaca ccgaaagcag tagcagaagt ttggatgctt actttgtaaa caacattgtt
    30661 ggtgagtata aattttcacc caaaatggcc aaaagcattt cactgcaggt tgtagttaat
    30721 aaccttttca acctgaaata tgaggctaac gcatgggtgt accgcacaaa atttcaaagt
    30781 ggcgatccgg agtatgtcag cataggctac tatccccagg ctacccgaaa tttcatgctc
    30841 cgacttgcgg ttgggttctg aaattatatc aacaataatg aagagcatca tgcgatgata
    30901 ataggggtgg gcagcagcaa gcccgcccct gcttgccaaa aagattagga ataagttgaa
    30961 agaaaatgaa ttaattgaac cattaagcgt gagaagccaa atgcttgtcc taaatcttta
    31021 gaaggaactt gcccgtttgc cggttgagct tgccgaaggg tgaatcaacc agcaggcgta
    31081 caagttcttg aattttgaat ctgaaatctt caattcttta tccctgaaat aaaatgagct
    31141 ggctgatagg taactacatc gaacttctgg gtgccaccat tggcttgatt tatctatact
    31201 tcgaaataaa gcagaaaatt tggctatggc cattgggaat tgccacctcg gctctttaca
    31261 tatacatcta cttttcatca aaattttacg ccgacatggg gcttcaggtt tactatttga
    31321 taataagtat atacggatgg taccactggc tgcatggaaa tgttgacaat acctccagct
    31381 caaagcccct gccggttacc cgaataacat tgaggctact agtagttctt acgactattt
    31441 cgcttttact attcgttatt ttatggtttg tgctcgacag gtacaccgat tcccctgttc
    31501 cggttggcga tgcctttaca acggcgctca gcatcactgc cacctggatg ttgacacgaa
    31561 aaattatcga acactggtgg gtttggattg tagttgatgg ggtctctatg ggactctaca
    31621 tctataaagg actttaccca accaccgtgc tttttgcttt ttacaccatt ttgtcggttg
    31681 tggggtacta ccaatggaaa agggaaatta ccccatctca agtaaaaact accgctggct
    31741 cttccgcaaa agagtaaatt tgctagtcca tataactcaa acaagtgccc tatgaacgct
    31801 gtaattgttg ccaacggcaa atttccggaa caccctaaac cgcttcgtct tatacatgaa
    31861 gctaaaacct tactttgctg cgatgggtcc atagagcatc ttgttaaact cggtataacc
    31921 cctaccgcca tagttggcga ccttgattcg gttagccctg aactcaggga gcgtttcaaa
    31981 aacatacttt ttcacgaccc cgaccaaaac agcaacgacc taaccaaggc tgtttcctgg
    32041 tgtgctgagc gtaacatcaa ctcaatttcc attgtaggag caactggaaa acgtgaggat
    32101 cacacagtgg gaaacatcgg gctacttgtt aggtatgcca gaatgggctt acaggttgaa
    32161 atggtcacag acaccggcac tatcaagcct cttttagaat cagctaaatt aaccagcttt
    32221 cccggacagc aaatttcaat cttttctccc gttaaccaaa ctacaattac cacaaataac
    32281 cttaaatatc ctattgataa tggccagatt cccgagtggt ggatgggtac gctgaatgaa
    32341 tcgctgggca gttggttcga actcaatttt tctcctggtc cccttctcgt ttatcaaatg
    32401 tactaataaa ctgtaatata tactagtttc tgaatatcaa tatattataa attttattta
    32461 caatatcatt gctttttgat tgcatattgt tagctttgtg gtgggactaa ttaaatctcc
    32521 acatcatgaa gtatctaaat tccgttatta ccggaacagg tagctacatc ccttccgtta
    32581 agattagcaa ccgcgaattt gcccgtaaca ccttctttaa aaaagatgaa tcggcaattg
    32641 aaaccctggt tgatgaggtt attgctaaat ttaaagatat taccggtatt gaagagcggc
    32701 gctgggtaac cgacgatttg tgcgcctcgc acattgcagc cattgctgct gaagaagcta
    32761 ttgccactgc gggcattgac cgcgaaacta ttgaccaaat catagtttcg cacaactttg
    32821 gggatgttct tcagggtacc attcagtccg acaccatccc cagcgttgca tcacgtgtta
    32881 agcacctgtt gaaaattaaa aatcctgctt gcattcctta cgatgttata tttggttgtc
    32941 cgggttgggt tcaaggggtc attcaggccg attcatatat taaatcaggg ttggccaaac
    33001 gatgcctggt gattggtgcc gaaacccttt cgagggtcat tgacaagcac gaccgcgact
    33061 gcatgatttt tgctgatggc gctggtgcta ccattgttga ggcggttgaa gctgacgaaa
    33121 aagccggggt catagcctcc tcaatgatgt cgcacaccga gcaggaagtc ttctaccttt
    33181 cactagggaa atccttccat cccgattcgg attcccgcat acggtacata aaaatggatg
    33241 gtcgtaaaat ctacgagtat tctcttataa acgtaccagg tgccatgcgc gaagccatcg
    33301 aaaaatcagg gattaaccca agcgaactta aaaaaattat cattcatcag gctaacgaaa
    33361 agatggatga ggccattgta gcacgtttca tcaaacagct aaaaattcaa acccccattt
    33421 ccgatctcat gccaatgagc attaaggagt taggcaatag ctcggtggca accattccaa
    33481 cactttacga cctaatacta aagggaaaag tgcccaacca taccatagcc aaaggcgata
    33541 tcataatgtt tgcttcggtt ggtgccggga tgaacatcaa tgcgattgtt taccgacagt
    33601 aatttagcaa cacacgatga acttttgact gaaaataacc agctaacgca aatggctaat
    33661 gcttgtttgg cgggcctctt ttatggtggt tattccagca taggtttttt cctttgatgg
    33721 gtagcttgct cgtaggagta ggcaatgctt aatagttccg cctcgctcca ggctttcccg
    33781 ataaacgaaa tatttacggg gaggccatct ataaatccca taggaacgct tatgcttgga
    33841 taaccagcaa tcgctgcata cgacgaactt ccccctaaaa agtgatcgcc atcaaccaaa
    33901 tcggttttcc aggctggtga accagtaggt gcaactagtg catccagctt gtattcgttc
    33961 attatacggt cgattccttg cttgcgcatc ccagtttgca tcatctcaag attttttttg
    34021 tactcggggc tgttaagatc tccttttgat tgtgccattt ccagtagctg ctggtcgaag
    34081 tacattaact ccacagaatc gttcaggtta aacttaatca gttcgctaat attctttacc
    34141 cgagcgctct cccccaggct ggcaaagtag cggttcaacc catctttaaa ctcatacagt
    34201 agaacctcga acgaagcgtt atcaacttcc ctgcttaccg gaaaatcaat agctaccact
    34261 tcggctccag cctgcttcat ggcgtcaaca ctttgtttca taagggtgtc aaccttaaaa
    34321 tgaaatcctg atacgttggt aagcaaacct attcgtttac ctttcagccc atctcttttt
    34381 aaaaattttg aatagtcaga atagaatacc gcatccgggt ttagggtttt tgcatcggta
    34441 gaatcgacac ccaccataat tcctaaacat actgccacat cctcaactgt acgcgccatg
    34501 ggtcctggtg tgtcctgggt aaatgaaatt ggtataattc ccgtccggct aatcaggcct
    34561 actgtgggct tcagcccaac aatgccgttg ttattggacg ggcacactat agacccgttt
    34621 gtttcggttc caattgcaaa agcacaaagg ttggctgcta cggcaacccc tgaaccggaa
    34681 cttgacccgc atgggtttct ttcggtatcg taaggattgc gggtttgccc accaactccg
    34741 ctccaaccac ttgatgagag actggctcta aagtttgccc attcgctaag atttgccttg
    34801 gcaataataa ctgccccggc ttcgcgaagt tttttggcaa catggctatc ccgccaaacg
    34861 tatgaattct ttaagacaac tgcaccagca gtagtcggca ttgaatcttt tgattctata
    34921 ttatccttca gtattaccgg tatgccatgc aagggaccac ggcttttccc tgcggctagc
    34981 tcgctgtcga gctcctgggc tattctcatg gcctcgggat taatagcaat tattgagtta
    35041 agaacgggtc catttttatc cagctcctct attcttgtca ggtaatcgtt taccacttgg
    35101 gccaccgtga actttccctc ccggtatccc tgttgaagct gttttatagt aatttcctca
    35161 aggtattcgg tttcccctac ttttgcctgt tcccgctcgt gacaacccgt agctaccgct
    35221 ataagtatga ccagcccggc tagacatgcg gcaatttttg tcaacttttc catttttgca
    35281 aattttttaa tagtgttagg acttgctttt catatctact ttacacaaac aatgctgtgc
    35341 tgagtgttgc tgcggcaccc tttttcgtat ggcataacta tgtcatatgc tataagtatg
    35401 ctttaatttt gaccaagtta gctcaactag gtttaaaatc aaatacatat ccagggtggg
    35461 gggtaatagg aaaaggcggt ttgctgaacc gccttttccg aattattcca ggttaaacgc
    35521 tttactttaa agaaaattca gagtagccaa tcatatagcc atccatatac agtgttacct
    35581 tataaacgcc cgtcaaaagt tctccattgt tatcaaaaaa tatgcacaca tcaagatcgc
    35641 tgttctggta atcgacctcc ctactagccg agaaaacaat tttttcgccc tcaaaatcga
    35701 acaaatcggc ttccgacttg gtcagcacaa agtcatcggg tccaacaatc cgcatgtaaa
    35761 cattccgggt tcccggttta gcaatagaat tttcgcttag ggtaaagcat gcgcgaattt
    35821 tattcaccct gcgtgctcgg gttacctcgt tgcctttctg gtttacaccc atggctacaa
    35881 tatcgcgtgc cttaactatt gaaccctttg caacctgggt attgagctct tccgtttttt
    35941 gctcaagctc tattactgtt tgtttggcgg tggctgcctc ctctttcacc ttaacattct
    36001 ctgcaattaa ctccttgttc aaagcattca aggaatctat atcatggagc atatccttca
    36061 taattgaacg tagggtgcct agctcctttt ggtactcctt aattttggca tagctaatct
    36121 gcttcacact ctgaagctcg gcatacaact ttttggcatg ttcctgctcc ttttgaagtt
    36181 tttgatttag cgtgtcgttg gtagtctcca gctcgctgta gtcattcatc aacctcagca
    36241 aattattaga aattgagtcc ttttgtgcct ggagtatttc ctgtgtatct tttgcttgct
    36301 gcttttgctg gaagtacaca acagttaatc cagctataat caacagaagc ataaccataa
    36361 ttactcttag agttttaatg gttttggctg atttttcatt attctccatt tgtcttttat
    36421 tatttagtta aaactgttag gtttagtgtt agtttctgtt accaagggta agattttgaa
    36481 atacttcctc aagttgaagg tctctttttt gaagggtaag tagcgtgaat cctgcatttt
    36541 tggcaaaatt aaatattttg cctcgtaagt cttcgagcct gccagaggta atccaccagc
    36601 agttagtatc aatgagggtt gccgtttcca ccccttcaat agccatgata gcttcacgac
    36661 taaccttttc ggcaaactct acaactatgt tttgcctaag ccccgacgac aaagctttaa
    36721 cttcggtgtt ggttccatcg gcaactacta aacccttgtt aatgatgatg attctgctgc
    36781 agattgcttc aacttcttgt aggatatggg ttgagaaaat aacagtcttg tctctgccta
    36841 tttcttcaat aacgttacga atttcgacaa tctgattagg atccaaaccg ctggttggct
    36901 catcgagcac cagcacggag gggttgtgaa ttaatgcttg cgccaagcct acccgttgcc
    36961 tgtacccctt ggagaggctg ccgatctttt taccctgctc atcgcctata cccgtaatgc
    37021 caataagctc cgataagcgc cggcgctttt cctttcccaa cttgtagatg gaggccaccc
    37081 acgataggta ttccttcacg tacatatccg tataaagagg attattttcg ggcaaatacc
    37141 caatctccct tctaaaatca aatcgggtat cctgcgtgtt atgaccatta atatcaacac
    37201 ttccgctgtc ttggaaaagg tagcccgtga taattctcat caacgttgac tttccggcgc
    37261 cgttcggccc taaaagtcca accacctgac ctgttgaaat attgaacgaa acatcgttga
    37321 gcgctttctg ctctccaaac tgcttggtta tgtggcttat tcctattgac atcgttatac
    37381 taaacccgta aatgctagtt cttcgttcaa ataaacggaa ttgggaatgg gtttatttta
    37441 ttccaaactc acttttaagc ctatcaacat agtcgagctt ttcccacgta aaaaattcca
    37501 cttcccgctt aacaattttt tctccgttgc agttgctatt ctcaataaaa tcaacattac
    37561 gtatataatg gtcgcgccca aaatgcccgt aggcggcagt ttcttcaaat atgggatttt
    37621 taagtcccag ccgtttaact attgctgctg gtcgcagatc gaaaatggtc aatatttttt
    37681 gggcaatttc accatcactg aggcttactt tagctgtgcc atatgtatta acatatagat
    37741 tgataggcct ggctactccg atagcgtacg ctacctgtat tagcacttcg tcggcaatac
    37801 cagctgccac catattcttc gcaatgtgac gagcggcata agctcccgac ctgtcaactt
    37861 ttgaagaatc ttttcctgaa aaagctccac cgccatgcgc tccttttcca ccgtaagtat
    37921 caacaatgat tttcctgccg gttagcccgg tatcgccatg aggcccgcca ataacaaact
    37981 tgcccgttgg gttaacatgc agaatataat cgtccttaaa tagtttttga acccgctcgg
    38041 gaaaacgttt ctttgctctt ggaatcagaa tattctttac atcctcgtaa attctgctga
    38101 gcatacgggc atcatcggta tcaaattcgt cgtgctgggt tgataccaca atggtgtgaa
    38161 tgcggacgga ttggttgtta tcgtcatact caagggtaac ctgcgactta gcatcaggcc
    38221 gaagataggt catttgccgc ccctcgcggc ggatgccggc cagctccatt agcagcacat
    38281 gcgaaaggac taaagccaat ggcatatagt catcggtctc gcggcacgca tagccaaaca
    38341 tcatcccctg gtcgccggcg ccctgttcct cctccgactg gcgaactaca ccccttgtta
    38401 tgtcgggcga ttgctcatgg attgttgaaa taaccccaca cgattcagaa tcgaaccggt
    38461 actggtcatt cgtgtagcca atccttttta tcacccttcg ggcaacctct tggacatcaa
    38521 cataggcctt agttttaacc tctccgctaa gcactaccaa gcccgttgtt acgagggttt
    38581 cacatgccac cttggcctcc gaatccctcc gtaaaaactc atcaaggaga gcatctgaaa
    38641 tttgatcggc tatcttatcg ggatgaccct ccgatacgct ctcggacgta aataaatacc
    38701 ccattttaat aaaatttaga tggttactta attaaataac aagggaaggt ttgaacagtt
    38761 gcggcaaaca gcacgtttta gcatttatta ttgtggttgc aatcagtcaa atctctccac
    38821 attctaccgg caaagatacc acattattta tttttactct ataatttatg ttcatctatt
    38881 gggcaatatc caagttgaag tatataaaag gcttttgtta agtccatcac gatggatttg
    38941 cctaaaaaca gacatatagc taaactaacc ttctggttaa gcggctaatc ggcaggtata
    39001 gccatgcagc gattagcgta cccacaacat cggctatcaa atcaaaccag tctgcccctc
    39061 gataggtgaa tactttaagc tgaagcagct caaccaatcc tccatacatg atggaaacaa
    39121 ctatcccaac ccacctggtc ttttgcaata cgcttcccga tagtctaagc gtgttgtttt
    39181 ccgagatcac cagaagtgtt agcacggcaa aaaggccaaa atgaaccaac ttgtctatac
    39241 ccgaaacgct tacctttgga aatccggagg gtggcattgt gcatagaatc aaaataacca
    39301 acccccaaag aatcgaattc ctattcttgc tgaaaaattt tagtgccttc gccatcatta
    39361 ccagtataat ttggttgtca ttcttgtagc aagtataaag ctagtaataa tttggtaaac
    39421 aaattgaacc atacctgcgg ctctgccttt taccccttac caccaacgaa taaaatattg
    39481 tattgctatt tatatcaaca ccccaatgaa ttaaaatcta tttggcttcc atattaaatt
    39541 atgttaaatt atctattgtt gaccctgtaa aaattgttaa gcaccttgta gaataagact
    39601 tctttagact tatctacaaa atgacaacag agcttagttt gattatatcg ggcaagtagc
    39661 tatttttata ccataaaatt actaaaactc atggccatgt ttgacaagca gatttatgtt
    39721 gaccgcaggc ttaagctgcg aaaaaaggta aaatctggca tagttcttat tttgggaaat
    39781 accgaaagcc caatcaacta ccagggtaac acgtataact ttcggcagga tagtaatttt
    39841 ctttactttt ttggacttga caccccgggg cttgctgggg taattgacat tgaagagggt
    39901 accgattgcc tttacggcga tgattttgac attgacgaca ttatttggat gggaccccag
    39961 ccaaagttga aggatatggg tgaaaaggta ggcgttcttt ctacccatcc atttagagaa
    40021 ctctctacta ccttgaacag agccataagg cagggccgaa aaattcattt cctcccgcca
    40081 tacagggcag ataataccct cctcctggaa aaattattgg gaattaacca aaccattatt
    40141 aaaacctact cctccattga actcataaaa gccattgtta gccttcgctc agtaaaagaa
    40201 ccctgcgagg ttgaagaaat agaaaaggcg tgctctgtgg gttacaaaat gcatgttacc
    40261 gccatgaaaa tggcaaagcc cggcgctttg gagcgcgaaa ttgctggcga acttgaagga
    40321 atagctcact ctttcggata tatgacctct ttcccaacaa ttctctccca gcatggcgaa
    40381 actcttcaca accacgatca ctcacttacc cttgaggaag gacgattatt actggttgat
    40441 gccggagccg aggggattag ccattacgct tccgatttca ccagaacttt tccggttggc
    40501 gggcattttt cacaaaagca aaaggatatt tattctatag tgctggctgc caacaataaa
    40561 actacagaac tagcaaagcc tggcgttcct tacttcaacg tgcataaggc tgcagcaagg
    40621 gttattgttg agggtctttc tgctcttgga attatgaagg ggaattccga cgaggctgta
    40681 gaaaatggcg ctcacgcact ttttatgccc cacggattag gacacatgat ggggcttgat
    40741 gttcatgata tggaggacct cggcgaagac tttgttggct acgatgacga aatttcacgc
    40801 tccgagcaat ttggaacttc ttcacttcgc atggcccgca agcttcagaa gggctttgtc
    40861 ctgactaacg aacccggcat ctactttatt cctgccctta tcgaaaaatg gaaatccgaa
    40921 aagattaatc aggcatttat caactacgat gtactgtcca gctatctcac ctttggaggc
    40981 attcgcctgg aagacgattt actaattacc gataacggat gccgtattct aggccaccga
    41041 atccctatta cccccgaaga ggttgaaaaa acaatgaatt catagcaaaa ttgctgcata
    41101 gccgattcga aaggagcgtt ggcaacatcc acgctccttt tttgtttcat aaatagatag
    41161 cggacgtagt tttgcatgta acctccacta ccgtaatgct cattacggta aaaatccagt
    41221 taacatctta ccgtttactg ttaagttctt tattattgac tgttgaaaac tagtaatgct
    41281 tttttgttgt catgcattcc aatttaacta cttaataaat tctcgatacg tgtcaagagt
    41341 atcttacaaa tctatacctg aagttttaaa cagcttagct aactaaatcg ggtggtttaa
    41401 ttcggaaaat gttttaacat tgcccggcgt tacccgatgt taattacttc ttttatttgc
    41461 tgattaccag caataaccat cttttctttg tgttaataat ttgttatttg ccttttaata
    41521 ccgaattttg cattattgtt ttccgttttt taccaacaat ataaacaaca aataatcata
    41581 atttaaattt tttttaaata cttaaataaa tattaatgaa ttattctatt gttgataagt
    41641 tagggtatat tgatgaagat attgatagca acattgactt gaaagttgct atacgcaaaa
    41701 tcaaggagga aaaaaatgcg gttattcttg cgcactacta ccaacgaccc gaagttcagg
    41761 atattgccga tttcgttggt gatagtctag ccctttcttt aaaagctgca agtcttaata
    41821 gtagcttaat tttgtttgca ggagtgaaat ttatggctga gactgtaaaa attttacgac
    41881 caaattcaca agttctaata cctgatctaa atgctggttg tagtcttgct gattcatgtc
    41941 aattttctga ttttaaaaaa tttatcgatt tacacccaaa tcatattgta gttacctatg
    42001 ttaactcttc agctgaggta aaaacgattt ctgacatttg ttgcacttca tctaatgcta
    42061 taaaggttgt taaatcgtta aataactcta ccccgcttat ttttgcacct gatctaaatt
    42121 taggcagcta cgtaaaatct accctaaaca gatctaatat ggttctatgg aacggttctt
    42181 gccatattca taatcagttt atgttacagg atattattac tcttaaaact aaacatccga
    42241 aggctaaagt tatagcccat ccggaatgcc aaaattcaat tttacttatt tctgatttta
    42301 ttggttcaac taaaagcctc cttgatttta catacaattc aaataatact gattttattg
    42361 ttgttactga accaggtata attcaccaga tgaagaaatt atcaccacaa aaaaatttct
    42421 atttccccta tgttcaaaat gaagaatgtt attgtaatga atgtagctat atgaaattaa
    42481 ataacttaaa aaaaatttac ttaactttaa aatatgaata tccaattata aatttagaac
    42541 ccgatgttat ttctaatgca gctattccta ttaatcgaat gttaaaaatt aactagttaa
    42601 acatatggaa aatcaaattt ccgacaaaga atttgaaaac aatattaggc catccgaatt
    42661 caaacaattt aatggtcagg atgatataat aaaaaacctt aaaattttcg tacaagctgc
    42721 caaaattagg tctgaatcat tagaccatgt gctgctacat ggtcctcctg gactcggaaa
    42781 aactactctg gcttatatta tagcgaatga acttggagtt tctataaaaa ctacatcagg
    42841 acctgttctt gaaaagcctg gcgatttggc cggaatactt acaaatttag ataaattcga
    42901 tatacttttc attgatgaaa ttcatcgttt gagcccaata gtagaagaat acctttactc
    42961 tgccatggag gattttagaa tagatatttt aatagataag ggtccaagtg ccagaagcat
    43021 ccaaattgaa ttgaatccat ttactcttgt cggtgccact actcgaagcg gtttacttac
    43081 tagtcctctt agagcacggt ttggaattaa ttttcatctg gagtactatg acgctagtac
    43141 tataaaatct atcattaaac gatcctctta tattttgaat atcgacatag atgatgatgc
    43201 ttctatggaa attgcactta ggagtagagg aactccccga atagccaaca gtttactacg
    43261 tagagttcgc gattttgctc aggtggtagg aaacggccga attaatcttg atatagcaaa
    43321 aagttcttta gatgctttaa aaattgattc gttaggtctt gatgaaatgg ataataagat
    43381 attaacaacc ataatcaata aatttaatgg tggacctgta ggaataaata ctatcgcaac
    43441 tgctattagc gaagaccctg gaactattga agaagtttat gaaccttttc tgattaagga
    43501 aggtttacta aagcgtactc caagaggaag acagacgaca gatttagcat attcccattt
    43561 gaaaattgaa aaaaatttta ggcagggaac cctattttaa aaatgaatta gaaatacttt
    43621 ctagtttaga ataattacca attcctattt ttttaccctt taagctaatt agcccatctg
    43681 ttttaaactc gcttagtatt cttataactg attctgttgc cgttcctatt atgcttgaaa
    43741 tatcctctct ggtgaggtta acatttataa atccctcctc atccttacca aatgtttgat
    43801 caagcattag tagggtttct gctagccttt cccttactgt tttttgagct atatctttta
    43861 tcaggtcatt ggcagtattc aattccttac aggttagctg gattagtgat aaggcaaaat
    43921 ttgaattttt cctaataaac tcaaatatca agctggatgg tatgtagcat acttcgctat
    43981 cttctagtga ttctacagta gtacatgctg gttcattact caaaactgat ctgtagcccg
    44041 ttatatcgcc tttttgagca aaagctacta tttgaggttt tgagtctatt ccggtcttat
    44101 aaattttaaa tactcctttc tttacacagt aaaaccctgt aattctgcta ccttctttat
    44161 aaattatttc tccctttttg tattcgtaac attccaaatt tgcttctagt tgttctgatt
    44221 cttcggtagt caaagaacca aaaaccgttt tcgacgacag gctacaggtt ttgcaaacgt
    44281 tcatgctttt cattacctac caaatttttg actatagaaa atcagtaaaa attcgtaaat
    44341 tgatttaaga accttatatc attttcaaaa taatttctta agtcattaac cctaaatttt
    44401 aacatggcta ttcgctcaat tcccatacca aatgcaaaac ctgaatattt tttactatca
    44461 atgttattaa gttccaacac attcggatcg accattccac aacccaaaat ttcgagccat
    44521 cctgtatgtt tacatacaga acatccttta ccaccacata aattgcagct tatatctaat
    44581 tctgctgaag gttctgtaaa tgggaaatat gatggcctca atcttatact tgttttactt
    44641 ccaaacatct ctttaacaaa gaactctagt gtttgtttca aatctgcaaa cgatacggtt
    44701 ttatctatat acagaccttc tacctgatga aatatgcagt gtgctcttga tgaaattgcc
    44761 tcatttctaa atactcttcc tggacaaatt acccttattg gcaatttttc attttccatt
    44821 gatctcacct gaacggatga agtatgagta cgtagaagta catctggatt tttttgtata
    44881 aaaaatgtat cctgcatatc acgtgctgga tgttctggtg gaaaattcaa tgctgagaaa
    44941 acatgccaat catcctcaat ttcctgtcct tcatatatcg aaaagccaat tttcctaaaa
    45001 atattgaaaa tttcattttt aactattgtt attggatgta gtgttccgaa atttagctga
    45061 ggttcctgaa gtgttaaatc tattttatct tctttttttc ctttagattt atatgtatct
    45121 tttaaaatat ctaatttttc atttaacttt tttttcaagt catttagcaa tttcccgtag
    45181 tatttttttt catcagtgcc tagattcttt agatcgtcaa atagttttgt caattcaccc
    45241 tttttcccta taaggctaat tctaaaactt tcaacttcct catagttttg aggagtaaat
    45301 ttgtcaatta attctaaaat attatttatt ttttcctgca tagttaaaaa ttatttagat
    45361 ttaacgtatt tttttgactt cgtttattac aatatcaacc agcggtctgt cgttttgatc
    45421 agtttttacc tccgatattt ttttaacaat gtccatacct tttgaaactt caccaaaaac
    45481 agtataattt ccatcaaggt gtggtatgcc tcctatcgtg gtgtatatct ctctctgttt
    45541 atttgtaaat ataaagggtt tttctgacga ttttacagaa tcgaccatgt tttctattag
    45601 aattagaata tctttaatat tcttatccga aacagctgta ttttcattca ggtatttttt
    45661 atataaatct tgttttattt tatttaattt ttcaaaattt atttttttct ctaaattatc
    45721 gagttcctcg tttgtgtaaa cttttcctat aactatataa aattgcgagc ccgatgacaa
    45781 tttatctgga ttaatatcat ctgactctct agccattcct attgcaccat atttatgaat
    45841 aatagtatcg caaaattcgg gatcaatcag atatcctgaa tctttttccc catattcttt
    45901 acctggttgg gaatttttag tttcagggtc ccctgcttgt attacaaaat ttttaataac
    45961 cctatggaaa atttgcccat cgtagaaatg ttggtccgta agattaataa aattaagtgt
    46021 atgtttaggt gtttcgttgt atagtacaac ttcaatatca ccataatttg ttgaaatttt
    46081 aacaagatgt cgattatcaa ctgtggcatt tttacagcct gttagtagga gcaaagcagc
    46141 tattgagaaa tacagcaaaa tatttttgtt catagtagta atattaaaat ttgttcaata
    46201 ttaatgaagt aaattacaaa ttcattcatt aaaaaaattt agttgaataa atttaagatt
    46261 gcgcgcatat agtactttat atttcgcaaa attatatgaa atattttaat tttgtgcgtc
    46321 aaattttatt tatggcttct gttcgtcaac tttactcaca atctgttgtt tatggtttaa
    46381 gcactgttgt tccaaggctg cttaactacc tgttggtacc acttcacaca actgttctta
    46441 ctaatcctga acagtatggg gtgattacag aaatgtatgc gtatatttcg tttattctta
    46501 tacttttaac ttttggcctt gaaactggtt tttttagatt ctcttccaaa tttaaaaata
    46561 acgagtctgt ttattctact attttttatt ttttaatttt cacctcttca ttattttttt
    46621 taataatttc tatattttca ttagatattt caaacttttt gggtagtttt tataaaccta
    46681 tttatataac tacacttgcc ggggttgtta gtattgatgc tgttttagct ctcccatttg
    46741 caaaattgag aaatgaaaac aagccatttt tattttctag tttaaaaata ttaggtgtag
    46801 ttattaatgt attttgtaat attctttttt atctcatcat tcctagaaat tctttatata
    46861 ctttgttccc aaatgtcgac ttgttatatt ttgttttttt atctaactta atacaaaata
    46921 tttttgtatt aattttattg ctaattttta ccaagattcc aaaattaatt tttagtccca
    46981 agcttcttaa atcaatttta atttactcat tacctcttct aattgctggt ttatcaggaa
    47041 ctactaatga agcatttgat agaattttta ttaagtattt atatccaata aaagacgaag
    47101 cactttatca acttggcatt tatggagcaa atgtaaagct ggcagttttt ttagtattgt
    47161 ttatacaaat gtataggttt gctgctgagc cttttttctt taaattgcaa aatgaaagta
    47221 ataataagga aatatttgct aaatccttca agtatttttt aatattttgt ttttttatca
    47281 caatacttat aacttttaat atccctattc taaaatattt tgttggttca aattatcgtc
    47341 ataatcttta tattgtccct attttactta tagcaaattc actttttggt atatacttta
    47401 atctatcatt ttggtttaaa ctctccgata aaactttatt cggtcttaaa tatgtaggta
    47461 taggtgcttt tacaacaata atttcaaatg ttattcttat tcctttaatt ggtattttcg
    47521 gtgctgcagt ctctagaata attagctaca ctattatgac gttactttgc tatatagatg
    47581 gaaaacgttc aggatatatt tccattgata gaaataattt aaagatattt ttattgtttt
    47641 tattatttat ttcatttatc tctacggttt tattaatata tagcaaacct ttattttctt
    47701 taatttttgc aaatttattt ttaatattat ttatattttt ctttttaaag aaggagaata
    47761 taaaaatttt aaatttacga aaatgctaaa agttaaaatt gtaaataaat ctagcttccc
    47821 tcttcctggt tatgctacat cacattcaag tgggatcgat ttatatgcta atattagtag
    47881 ttcaatcaaa attttatctc ttcagagaat tttaatacca actggaattt ttgttgaaat
    47941 tcctgacgga tatgaagcgc aaattcgtcc gcggagcgga ttagccctta aacatggatt
    48001 aactgtttta aacacgcctg gcacaattga ttctgattat cgcggcgaaa tttcaataat
    48061 acttataaat ttaggtagtg atgaatttat acttgagcct ggtataaaaa ttgcccaaat
    48121 ggttttttcc aggtatgaaa gagtacactt tattgaagta aatgagttga ccgaaacatc
    48181 ccgtggagcc gggggattcg ggcacaccgg gatttaatta ttttaaattc ttcagtaatt
    48241 tagaaggtta cagctgaaca gaaataaatt tattaatgtc atattttaaa ctgtgaaata
    48301 tatttttaaa tattgtttgt tctttatttt atgctactgc attacatttc ctattaaatc
    48361 gcagtcttta gaggatttaa attctactaa gagaaaaatt gaaaatcaaa tacagcagct
    48421 aaatgtttta attaatgaaa atgcaaacct aaagtccgaa actattaaca atcttaattt
    48481 agttagccaa agaataaagt taaaaaattc attaatcaac caattagaat tagaaatatt
    48541 atacctcaat aataatattc ttcaaagcca gtcaaatatc gatagtttat cctttttact
    48601 gaatagcaga aagaaagaac ttgagaaaat tcttcaattt taccaaaaat atcataatag
    48661 ctacaacatc ttaatattta ttttatcttc caactctttt aatcagtcgt ttttaagaat
    48721 aaaattttat aattctatca ttaaatacta ttcaaatacc ataaaaagca tatctacgga
    48781 tattaataaa attagtgaaa taaaagtatc tctcgaaaat caaaaaaagg agctttacct
    48841 aaagcaactt caaaagattg atgaaattaa aactcttcga aaagacttag atttatacaa
    48901 ttcaaaacta aaaaaattaa aatctaatga gaaaaatttg agaaagcaaa ttgaaataga
    48961 aaaaagacga agtaatgaaa taatatctaa aataaatgag ctgattgaag aagaaaccag
    49021 aaaaaaaatt atttctacca ataaatcaaa cgaaaatgtt aaattaagca acgacttttt
    49081 aaaaaattat ggaaagttac cccacccggt tgcaaatgct tttgttatta acaattttgg
    49141 cgaaaccaat catcctgttt taaaaggagt aaaaatcaaa aataatggtg tagattttgc
    49201 atgcaaaact ggctccgaaa ttcgttcaat atttaatgga gaggtaaaaa aagtatttaa
    49261 tctacctatg aacggaattg ctgtaattat tcgccatggt aactatttat cggtatactc
    49321 caatttaact aatgtatatg taaaagttgg tgataaggtt attactgagc aggttatagg
    49381 taaaattgtt tcaaatgata attccggctc aattttacat tttgaaattt ggaatgaaaa
    49441 aaatccagaa aatcctctta tgtggattta ctaacttttc ctatttttgt aaccttttag
    49501 aatacattta gtataacgaa taaaatattt tgtatgcaaa ctattataga gttaaatagt
    49561 agtttagata gtatctcaaa agttgaaaaa tttatagacg aaatttcagc gaatgttaac
    49621 atttcatctg agatttatgg taaaattctt attgctacca tcgaagcagt taataatgca
    49681 atagttcatg ggaataaact taattctaat aaaattgtta gggttaaaat cgagtccgaa
    49741 gggaataggt taaaagttat agtagaagac gaggggccag gatttgattt ctataattta
    49801 cccgatccta caacgccaca gaatatcgaa aatatctctg gcagaggagt ttttttaatg
    49861 agaaaattgt ctgatgaaat aaattttttt aataatggta gtctagtaga gctaattttt
    49921 tacttgtgat ggcaatttca tttcaatcaa atataaactt taacttaagc aataaacgaa
    49981 aagttaaaac ctggattaaa aatgttgtta ctaagtatgg atttaaaatt ggagatgtaa
    50041 ctattttatt taccgatgat gagtatataa aagaattaaa tattaaatat ttaaatcatc
    50101 actattttac cgatatcctt acctttgact actcttcagg ttttgtttta aatggtgata
    50161 ttgttatttc aatcgatact gtaaggtcaa atagtatctt atactctact tcaatcgacg
    50221 atgagctact ccgtgttata atacacggat ttttacatct tttggggttt aatgataagt
    50281 ctagtgaaga aagtagaaaa atgagggagc tagaggatgg tgctttaaat attttttatt
    50341 cgaatgactg atttttttga tattattgtt attggaggcg gacatgctgg atgtgaagct
    50401 gcttctatcg cagcaaaact tggatcttct gtattgctta tttctctgga tttaaccaag
    50461 ctggcccaaa tgagctgtaa cccatctatt ggtggaatag gtaagggaca aatagtaaaa
    50521 gaaatcgatg ttcttggcgg atataccgct aaagtaacag atcttaccac cattcaattc
    50581 aggatgctta acaaatccaa gggtcctgcc atgtggagcc ccagagcgca atgcgataga
    50641 atgctatttt cgtatgaatg gcgaaaatat ttagaatcta ttgataacct ccatttattc
    50701 caagacgaag ttgttgatat tgattttgat gttaaaaacc aacttgttag tggtgttacc
    50761 acccaaagcg gcattaaatt taaatgtaat gttcttatcc ttacaaatgg aacttttcta
    50821 aaaggaaaaa tccacattgg tagcaataat ttttccggtg gtagatttgg tgaaaaggca
    50881 tccatcatgc tttcggacaa attggaagat atgggattgc atattggtag aatgaaaacc
    50941 ggaacacctg taagaataga tgggcgaagt gttgattttt caaaactttt agaacaaaaa
    51001 cccgatactt atggcaagtt ctctttagat cctaaataca cttctacctt gcaacaaaag
    51061 tcatgctatg tagcacacac caatagtgtt gttcacgata ttattagaga aggtttcgat
    51121 aaatcgccac tcttttctgg caaaataaaa gggataggac caaggtactg cccaagtatt
    51181 gaggataaag tcagaacatt ttatactaaa gattctcacc aacttttttt agaacccgag
    51241 ggagtcaaca cttatgaaat gtaccttaat ggattttctt cttcactacc tgtagatatt
    51301 cagctaaagg cactaaaaaa tatcgagggt tttgaaaatg tatctatact tcgacccgga
    51361 tatgcaatag agtacgacta ttttgatcca atgcagctaa atcatacatt ggctcataaa
    51421 agtattaaga accttttttt tgcaggacag gtaaatggaa ctacaggcta tgaagaagct
    51481 gcaggacagg gattgcttgc tgggattaat gcacattact ttattaatga tagctcgaaa
    51541 cagctgatat tgggaagaaa tgaagcatat attggagttt tgattgacga tcttgttacc
    51601 aaaggagttg atgagcctta cagaatgttt actagtcgtg ctgaatacag aattctgatt
    51661 cgacaggata atgttgatga aaggttactt cctattgcgc aaaaactcaa gctggcttca
    51721 aatgaagaca tagataggtt taatgtaaaa tctaaaaaat tagaatcatt caaaaattta
    51781 attagcagca taacggtttc tcctcaagat gtaaatgatt acctggttaa agttaactct
    51841 tcacccattg aacataaacg caagctaaac gatatattgg ctcgacccga tgtaaatttt
    51901 ttcgaactgt tggatagttg cccgaattta atcggaattt taggagacgt tgataaaaat
    51961 atagccgaaa attatattat taaaatcaaa tattccagct atgaggaaag agagcaattt
    52021 atggttgata aaacccaaaa gttagaagcg cttaaaattc ctaataactt caattacgaa
    52081 tctatacgct ccttaagtac tgagtcaaga caaaaattat ctaaggttaa accgaaaaca
    52141 atagggcaag ctaagaatat tcccggtgtg agccccagcg acattaacgt tctgcttgta
    52201 tatttgggaa gataattgtt ccacgtggaa caaatgtttt gaaatttgtt ccacgtggaa
    52261 caacttaagg cattatgccg atttgtttta gggatttctt cttaaatttt ttatgttaaa
    52321 gaaacacttg tggagcgccc ctgaaatgct gaacacacaa gcgccctgca ataatcaagt
    52381 agccaactgc ataaacttga tgtctaaaac gaactggtca aaatatttta aatcagaaac
    52441 ataaaatttt gtaataatga atagatctgg cattgtgaaa ggtgttgtgt acaatccgat
    52501 actacacgaa tcgtttgggg cattggtctt tattgaaaat ggaaaaatcc tgaaaatcga
    52561 gagggatggg aatattaagg aaccgctaat acttcccggt tttatcgact cccacgtgca
    52621 tattgaaagc tcaatgctca cccctgcgca attcgggaga accgcagcag ctcatggaac
    52681 cgtagccgct gttgccgatc ctcatgagat tgccaatgtt gcaggggtcg agggcattaa
    52741 ttttatgctg agtagcgcaa tagagtcacc cataaaacta ttttttggcg caccgtcgtg
    52801 cgtaccagct tcaccattcg atgaatgttt tcagcctttt agcccaggta taatcaagga
    52861 gcttatcgat agaagcgata ttttcttttt gggtgagatg atgaatttcc ctggtgttat
    52921 cggtggtaac gcagaggtta tggaaaaaat caacattgct aaaagcgcag gtaaacccat
    52981 cgatggacat gcgccctcgt tggacaaaaa cgacttaaaa aaatacattt cagccggaat
    53041 ttctaccgat catgagagtt ttactctcga agaggcaatt gacaagctat cgtttggaat
    53101 gaaggtaatt atccgcgaag gtagtgccgc taaaaatttt gacgccctgc attcgctaat
    53161 tcctcagttt catgataacc ttatgttttg caccgacgac tgtcatccgg acgatcttat
    53221 agttggacat ataaacaagc ttgtaaaaaa agccatcaac gcggggtata atatcttcga
    53281 cattctccag gtttcttctg ttaatcctgt aatgcattac aacttaaatg tgggattact
    53341 tcagcccgga gatcctgccg attttattgt ggtcgatggt ttaagtagcc ttaacattat
    53401 ttctacatac ataaacggtg atgacgtgct taaggagcca aggccgacaa caaatgtcaa
    53461 aattccaact tacacttttc ctaattcgtt cgattcaaac ctgctcgtta aaaagctgga
    53521 cactaagcgt gttaaggtta ttgatgtagt taaagatgag cttataacca actggcatgt
    53581 tgagaactct aacaccgatc tgctggaagc aaacccaaag gaagatctct tgaagattgc
    53641 tgttgtaagc cgttataaga aaaatgaagc atctgttggt ctaatcaggg gttttggtat
    53701 caacaaaggt gccattgcag caagcattgc ccatgatagc catcatatca ttgccattgg
    53761 ttgtgacgat aactccatca cacaaagtat aaattatatt gttaagtgcg gaggtggaat
    53821 ttgctatttc gatggcgata gtatttatgg tctccccctt ccggctttcg gattaatgac
    53881 taatgaatca gccgaggtgg ctggaaaagc ctaccacgaa ttaacccaac gtgttattgc
    53941 tgatggctgt aaactgcaag ctccctttat gaccatgtcg tttatgtcgc tttcggttat
    54001 accccatctt aaaattaccc ccacctccct gttcgatgtt gataaattta gctttaccaa
    54061 tatatgccta tagcttatta aattagcatt tataacttta tctactagat gaatgacatt
    54121 ttatcgaata tcgttattaa cctcccggac tcgccgggtg tttaccaatt tttcgatata
    54181 aatggaattc taatttacat tgggaaagca aaaaacctta aaaaacgggt ttcatcctat
    54241 tttcatcaat cggcttacga atcggcaaaa cttagagttc tcgttagcaa aatatgtgat
    54301 atcaagttca ttgttgttga ttctgagtca gatgcactcc tattggagaa tattctgatt
    54361 aagaaacaca agccccggta caatgttttg cttaaagacg ataagaccta tccctggatt
    54421 gttattaaga acgaaccatt tccccgtgtt tataaaaccc gcaagtatat tgatgatgga
    54481 agttcttact ttggcccata cacatcggga aggctcgtgt ttgaactgct cgatctaatt
    54541 agaaagcttt accctgtacg tacctgctcg ctcaacctta ctactgctaa catacttgca
    54601 aaaaaattta aaccttgcct tgaataccat attgggaact gtcttgctcc ttgcatcggc
    54661 atgcagagcc atcaaagtta tcttcaaaat attgatagta taaaacgaat tttacagggc
    54721 aatctttccg aaataaagaa tcatttaaag gctgaaatgg ttaaatattc aaatcagtat
    54781 aagtacgaag aagcacagct gataaaggaa aagatcgaca tattaacgaa ttttcaaagc
    54841 aaaaccacta ttgttaaccc aaaatataag gatatcgaag tttttactat acttaccagg
    54901 cataacattg taggagtaaa ttttattaga ataaataaag gccaggttgt tcaatcctat
    54961 aacctacatc tgaaatcctt cgtaaacgaa tcgcttcccg aattattttc ttctgcaata
    55021 gccgaaatca ggcaacgtct caaggctaat tctactagaa tacttgtgaa tatcatgcct
    55081 gaatttacta ttaatggggt ttccttttca gtcccacagc gtggagatag aaaaaaattc
    55141 gttgaattaa gcttaagaaa tttagagata tttcttgatg acctctataa aaatgaagca
    55201 gctaaaagcc ctgtcttagc cgttgactcg attttgacca aaattaaaga cgacctaaac
    55261 ctgagcgatt tgcccgtcca catggagtgt ttcgataatt cgaatattca gggaagcaat
    55321 ccggttgcat catgtgtggt ttttaaaaat gctaagcctt caaaaaagga ttaccgtcac
    55381 tataacataa aaacagtcca aggccccgac gattttgcct ctatgaagga ggttgtttac
    55441 agaagatatt cacgaatgct ttctgatggc gaaacccttc cccaacttat tgttatagat
    55501 gggggtaaag gacagcttaa cgccgccatg gaaagtattg ttgagctcgg aattcaaaat
    55561 agggtacatt taattggaat cgcaaaaaga cttgaggaga tttttgttcc cggggatcct
    55621 gttccggtgt accttgacaa gcgctcagaa actcttcgga ttattcaaca aataagaaat
    55681 gaagcacacc gtttcggaat aaaattccac cgtaacaagc gaagcaattc agcatttgaa
    55741 tctagtctcg agcaaattga aggaattgga cccaaaacca tccagctact ctttacccgt
    55801 tacctctctc ttgatggaat taggaatgcg ccagaatctg agcttattga cctaatcggg
    55861 actaagcgaa cgaaccttat caagaattac ttcaaaaaag tttagtattt ttggggatta
    55921 agtattatta acaaccatat accatatact ccttactgcc atatatttgt aatggatagt
    55981 ttgaatttac gcattaattt atacgacaac ctaaaacaag cgtttttttc tcctgatacg
    56041 cttaacggtg ggtgtacaat tagtccatca gccgacacag ccttagcctc gtcaacaaag
    56101 ctaaacaatg ttgtttcttc tttgagtggc gatgcaaatg tgatggtcga gccaattctc
    56161 cgcaaaacta acaacattcc aaattcctac attattccat ttttagcagg gctagtggtt
    56221 ttagtagtca tcgtgttgcg ttaccgaaag tatctggcct ccatagttga atctgcttta
    56281 tattcatttt ttgcggagaa atttgttttg gaaacaaatg ttccattccg ccgaactttt
    56341 gcgctgctcg atgctctgtc tgtggtttcg tttagctgct tttctttggt tgcattgaaa
    56401 ctgcttaaca ctgaattttt ttatgaatca aaacctataa ttcttattgc cattgctata
    56461 gcctcttttt ctctattcag attattcatt agtatatata actacttgat aaatagcata
    56521 ttatccaaca atccgatttt ttctttattt caatataact actttatttt cataagaata
    56581 gtaggattta ttattcttcc aatttcattc gttgcttttt atgcccctac ttcggtagga
    56641 ctttacctcc tctattttat gtttggtatt ctactagtta ccctattcta taggctatac
    56701 cggcttactt taatatttgt aaaaaatcgt atttcaattt tgtatttcat tttatacctt
    56761 tgtggcctgg aagttgtccc aattcttatc gttattaagg aggtacaacg catttaattc
    56821 gtgttttaat attataaggc tttgaaaatc agcagaattg tcgtagccca gcaagaacct
    56881 gttaacgaga aatctccata tcttgatatt tctaataggt atggagttaa aatcgacttt
    56941 cgtccattta tacatgttga aggaatctca gctaaagatt ttcgagctca aaaaattaat
    57001 atttccgagc acactgcagt aatttttacc agtaaaaccg ctatagacca ttactttagg
    57061 atgtgtgaag agttaagagt caccattccg gaaacaatga agtacttttg cattacagaa
    57121 tcggttgcgc tttacctgca aaaatatata gtgtatagaa aacgaaaggt gttttttgga
    57181 aacaatactt ttcccgatct gctcgaaata attcaaaagc ataaagagga atattttctg
    57241 gtaccccttt cggatattca taagcccgaa attcccaagg cgctcgagaa agcaaaatta
    57301 aaattccgga aaggggtatt ttataaaact gttagtagcg atttatccga cctaaaccct
    57361 tttccttacg atatggttgt tttttatagc ccaagcgctg ttaaagccct atttgataat
    57421 tttcctcagt tcaagcagaa tgatgttgtc atcggtggct ttggaaaaaa tacggcaaag
    57481 gctatcaagg acgccggttt aaggctggac attagcgcgc ctaccactac ctgcactagt
    57541 atgaccctgg ctatagacaa ttttttaaaa aatttaagca agaaatctaa gtagttaagg
    57601 cacttcctac ttaatttgta tgaaatattt cttgcgaaag aacaatcgct taacgctaca
    57661 gcaccaggtt gaccttcttt ttagccatgg ttatgggtat tttatttacc ccatcagggc
    57721 aaaagttctg ctatacgatc ggaatgttga agaagacaca tggtttaaag taatggttgt
    57781 tgtaccaaag aggcatgtca aaaaatcaag cagcaggaat cttataaaac gaagaataaa
    57841 ggaatcattt cgggtggcaa tacattcatt aaaaggttct tgtccttcaa acaaaattat
    57901 cttaatagct atttctttcg tttcaggcga tattaagccc tacgatagaa taaaggttag
    57961 cattgaaaaa ttgatgaatg aaatcgaaaa cgacatagaa aagttaaaat ccataaagga
    58021 ttaaacattt ttcaaggcga atgctttaat agttgtttat taagaattat tttgtgttga
    58081 atttcatgat atgaccaaga ataaaatccg tgatgtttta attcttttac ctttagggtt
    58141 aattaagttt taccaacttt ttatttcacc ccacatcccc aatagctgcc gttatactcc
    58201 aacctgctcg gtttattcgg tagaagcgct aaaaaagcat ggccttttta aaggaagctg
    58261 gctttccata aagagaattt taaggtgtaa tccgtggggc ggtcatgggt atgaccccgt
    58321 tccttaatct accgcttttt taaaactatc ctaaatgtag ttccaactcc aggctctgaa
    58381 ttcaggacaa aaattttccc cccgtggtgc tgttcaataa tacgcttaga aagggttaac
    58441 cctagtcccc agcctctatc ttttgtggtg aagcctgccc gaaatatttc ctcaaatttt
    58501 cccttctgta ttccccttcc cgtatccgaa atatcaataa atacaacctg ggtattgtcc
    58561 gtaactttaa aggttatctc accctttccc atcatggcat ctgcagaatt cttaacaata
    58621 ttctctataa cccattcaat cagaatgcca ttcatgcgga tgttcaattt ttcctgaaca
    58681 ttatattttt ttataacagt cacatttttt aatactctcg ctcccagata gtcaatagtg
    58741 ctattaagca gctctattag gtttgcttcc tgaagtattg gagttgagcc aattttagag
    58801 aatcgcccag ctattttttc caaacgcaca atatcttttt caagttccat tatgataggg
    58861 tcatttggta ttttttcttc cagaagggct aaacagccca ttaacgaaga ggtaggggtg
    58921 ccaagctgat gggccgtttc ctttgccatc ccataccaaa tgctgttctc gtgtgctttg
    58981 cgcgattgac tataggcgta atatgaaatg agaaaaaaga gaagaattat gacgatttgg
    59041 atgtagggga aaaattctag ccttcggagg gtggtgctat cccggtagta cacatagttt
    59101 ttcgtgctat cggatagcat aatttcaatg ggctcatgct ctgatgtcat ttttctaagt
    59161 agcctataaa ccgctactga gtcttttatt tgcctctcgg aaatgttcag gtgcgaaatt
    59221 attcttccgc tttcgtctgt aagcaaaacg ggcaaggttc tattattttg tataacctct
    59281 gaaacaaagg ttaaatcttg gcatgaatcc tgggtagtgg agagaaacct tacgcctttg
    59341 gcccataaat ctattttggc atgctcctct tccgataaat tttttacaag cttattgctg
    59401 accagtagcg aaaaaatcac gataaaaccg gcaaatataa taagcgcaaa attttttaag
    59461 gtattacccg aaatactcat aaacattatg gctatttagt tttagttgta tcatattcat
    59521 cccactcaac ttgaaatttt tgagatgttg gttgttgctg tatagcgtgt tgcttcttta
    59581 aagaatcttc aaaggtttgc ttaaattcgt tgcttatgat ttcttttaac gattgaccct
    59641 ctgtattaat tgtgttttta atggcttcgt agctgctttt tttgtcaaga aaaacctcat
    59701 agcccttact attacccttc agggttaagt ataggtttat tgcgccgctg ctagttttag
    59761 gctggttata gtctgctgaa taatttaaga acctctgggc cagtaagctg gaaaagttaa
    59821 tacgagcatg gtattcataa tcgtttgtca ggaagtgctt tcccgaaagg tttaacccca
    59881 taacgtttga atttaaatcc attgagggga tgtaaataca gccattcgaa atcaaaatgt
    59941 tattgtgaat agtggtaaaa tctatcgatt ttacctggtc gagtttaagc cattttgaaa
    60001 gcttttttat gttattcatg cctctcaatt tgccatcgtt tatagtaatc tccgaattca
    60061 tttttgccct gtcccaccta atgttgttag cactatccag ctcaaaatca aagttgcaat
    60121 gccccgagag atgcccttct atgttgttat gggtaatgta cttttggttc cagctgttat
    60181 aatgtctgaa aagttcggta atgtctatac cctccatcga tgcgtttata gagtacttat
    60241 caccaacgac cattaaatca ccatcaattt tcccttcgaa acccttagca tttagccctt
    60301 taacatatat actatccccg actatattca ttactcccgt tacattttca aaggcatagt
    60361 tggtatatat aagtttttta gtgttgatgc ttgtttcaac tgtcgtataa accctttttt
    60421 taccaccttt atttacggta gaaactgatt ttattaagct atctaagttt aaatagctag
    60481 aatttaaata tattgatgga gaaaaattta acggattatt taaatagtaa atggcattat
    60541 tctgacacaa tgtaaaatca atttttgagc tatccaaata gcacgtagcg ctaataattg
    60601 ccttatcgcg aattgtcgct tttcccgaaa tgctagacaa atttttgaat gcagaaatcg
    60661 aatcaaggct gaagctaatc tcgctggcta catcatgcaa caaaaatcca aaatgtttac
    60721 tttgtagccc gttacccttt cttagctcta gtaaagcctt aaaggtaccg ttgccttcaa
    60781 gcccagttag tttattagta aatgaaggtt cattttttcc cagctttaat aacccctcgg
    60841 cgagtattat tattcgattt gttccatagg ttctccccga aaaggcagct tcgatattct
    60901 cgtaacgaac cactgcattt gatacttgcc cggaatgtgt ttgcaggttg ttggtaaagt
    60961 aacttaagcc gttaatttct ttaatctcaa taattttacc tttagcaata atacttacat
    61021 tctgggcatc gtagtctatc cataggccgg ttaaattttt tttacctcca ttatacgaca
    61081 acttgcccga cattgcgacc ttacccgtta tgtcggcagg caatcccttt atattggcaa
    61141 cttggattaa ccggttaatg tttgtcctgt tcagtttaaa acccaggcca agggtgctgt
    61201 tttccaggtg atacaatccc tgaagttcta acctgctacc acttaggcta atttttacat
    61261 ttcgcaggaa gacgcttttc ccatcatagc tagctattgc atttacctgt gtttgttcat
    61321 ttagcataaa cttacttcct aaatttattt ccttaagctg gcaatcaagg tttaacgtta
    61381 aaattttttt tgctcttatg ttaagagtaa gcctatctat tccgattaat gatttttgcc
    61441 tgctaagtgt atcctctatt aatgctttaa agttcgaaag tgtaaaccta ttgatgaaga
    61501 actcgttttg gagaagggct gcttgtccgt tagcaggatt tatatcgttc ttttttagta
    61561 ggtttgtttt tagatttagc cttccatttt ctgctttaca tgaattaatt tcgaaattcc
    61621 ccagcagtag ccttgctgca ttaattttta aatccaagtt ttgcgccgac atgagcgtat
    61681 cattattccc attttctggt agtgtaagta tgaagtctga aaatgaaaag gttgcaaatg
    61741 gaaaattttt aaacgtatca aacttaatac tactgtagta tatgcttact aactcatatt
    61801 cattaaactg ttttaataac aaatcaatca atttgctctg atttataaat acataagttg
    61861 taagcaatat tagtgctaaa attataccac ttaaaaagta aagggtaaaa agtaatcctt
    61921 tgcgagcaaa tcggcctaaa aaattaaaca gcggtttcat tcttgctatg ttttcagttt
    61981 tccggttaca aacacttccg ctcttaacct tattttaaaa ggcgttgagc ggaatgaaca
    62041 tccatatttt ggtttcaccc agccagcagg taacgccagg tagtttgagc attgttaagc
    62101 ctgctaccca atattgatag cggtatggct ttagtataga aaatatgtat ttgtttataa
    62161 ttgaacggtg actctagcga gatgttttat aatctaatat tttgacatac cacgctaata
    62221 gcaaccggca tattataaat acttcctaat cctatacatt ttggcaaaat ccctaaatgt
    62281 taaatagatg ttgggagatg caagcaaagg gaaaggcttt agcaccagtt tacacgccca
    62341 ttaaaaatcc acaaatttat ttttttgctg gtaaataccc atattttttc tatacgacta
    62401 gtaattaagt ataaatacct ttaagataac gaataagcac attccgataa attaatagtt
    62461 taggtagtgg tatatttttc ggtcgtctga aactgttaaa aggttataag aaattattat
    62521 gtgctttgta gatcgattaa aatatataaa tttacctgtt tcttttaggt gaatatcaac
    62581 gtgaagcatt tgccaggaaa gcaaaattaa gatttatgaa atttcggata gtttctctct
    62641 gtttattatt tgccttcagg gtaggcatat cacaaaatat cgattccctg ctgctaaagc
    62701 tgtaccagga gtcaaatgat aggtcgagga tcgaaatctt actaaacata tcatcttttt
    62761 acgatcagca tgacagaaat ttcatacagg ccgaaaatta tctatacgag gcattacaga
    62821 tagcccagac agctggagac aatactcttt tagccaacac ctataacgag ctgggcaagt
    62881 tttatagaaa tcagtcaaaa tatccggaag cactcaaata ccatcatctt gctgactctt
    62941 tggccgttaa gatgggtgat tgtattcttc aggcgacaac attaaacaac ataggtgttg
    63001 tctatcgaag actcgacaac catgcccaag catctgaata ccatctaagg gcacttaata
    63061 ttgccgaggg atgtaaggat acattcaata tttccgttgc ctgtaatagc ttaggaaata
    63121 ttttttcact taatggacgt taccagcagg cactttcata ttttaacagg gcgctggccc
    63181 tatctattag ccagaataac ctacttggcc aagcgatgaa cttcaacaat ataggtgaag
    63241 tttatgagtt tatgggagat tatagaaatg ccaagtacta ctacaccaag tcgttggata
    63301 tcaatttcaa gttaaaaaat ctaaagggaa tctcaatcaa ctataatgcg cttggtaaaa
    63361 tagaaatgtt taatggtaat cccgaaaatg cgtacagact tttccaaaac gctcttgaca
    63421 tagatagaaa gctaggtgat aaaaagtttc ttgccgatag ctatgtaaac ctggctaaag
    63481 cctgtctggc cctaaaaaaa attgacaagg caaaaatcta cctggacagc acaattacca
    63541 tagcccaaca aataggaagc ccttggcatc tccagtctgc ctatgaagca tactcggatt
    63601 actttgttca aaaatcagat tatttatcag cgttaattag ctttaagaac gcttcacggt
    63661 tcaaagacag ccttttaaac gagaaaaatg cacgacatct ttcaacaata cagacaattt
    63721 acgaaaccga aaaaaaggaa aaggaaatta gctacttgct gcaagaacag gcactaaagc
    63781 aaaaagaact tgaaaagaat aacgcacaga aaaatgcgct cattattgga cttgtactaa
    63841 gctctttagt tgttattgtt tctctgtttg catggagaac aaaggtaaaa tcaaacattg
    63901 ttctatccga tcaaattaag gagattgagg ataaaaatcg gctccttgaa attcaaaagg
    63961 aagaaattga acaacaaaaa attaaaatag aaagcaacca ggcctttatc gagcaaaaaa
    64021 ataaaaatct cgaagaggcg cataagatta ttgagagcta catcgaaaat attacagata
    64081 gcattaggta tgcagaaaaa atacatgagt caattcaacc cagcttgagc gatatgctat
    64141 ccattttctt agatgcgttt atttacagca agcctaaaga tattgttagt ggagattttt
    64201 gctggttaac ccgaagggat aacctggttt ttctggctct agccgactgc actggacatg
    64261 gcgtcccggg tgcttttatg agcacaatag gtattaacat tctgaatcaa gccattaacg
    64321 aacaacacct ttactctcct gataaaatac taactttcat aaatcgtact ctcattaata
    64381 gattaaataa gtcaacagga gagcaggttc tgaaaaacag catggacata gctgtttgta
    64441 cttttgatac cgaatcgctt ttactgagct attctggagc gcttattcct atttttatcc
    64501 aaaaggggaa ttccgttgaa gaagtaaaac ccgactctat tacccttggc acaaacttta
    64561 gccactcagc atcgggcttt tcacttcaaa acattcagct taatcatggg gactgggttt
    64621 acttatcctc cgatggtttt tttgaccaac ttggtggcga atcacgtaaa aaattcacgc
    64681 gaaagcaatt catgcagata gttgcggata gtcctaatat gaagggtaat gttaaggtag
    64741 atataatcga aaagaaattt ttgcagtgga tgaatagaac cccgcaactc gacgatgtgc
    64801 ttgtttgggg atttcaggta tagagcccaa atattattta agaaaatttt cggactgttg
    64861 aaaatccccg atacaacatc cctacaactt aaaatttcca atacccacac catcaacatg
    64921 aacgaaaaca ggagcatcga acgcaaacgt ttttgcatca gcctttttgt atcgcttatt
    64981 ttcattgtag cgctttggtg cattaaaatc tttgaaatag ttgagggtat ttcactctat
    65041 aggctgggtg tttttcctag acaaataagc ggactgcctg gaataatttt ttcaccatta
    65101 atccattcag atttcaacca tctgataagt aattcgttat cgcttttatt cttacttatt
    65161 ggattactat atttttatag agacctatca tataaggtaa tattgtttat ttggattaca
    65221 agtgggctaa tggtttgggt tggagccagg gacagctacc acattggggc tagcggtctc
    65281 atttatggat tggcctcgtt tcttttcttt agtggtgtgg taaggcatga tcaaagactt
    65341 atggcaattt cgttgattgt ggtgtttttt tatggtgggt taatatgggg aattcttccg
    65401 gtttatccag caatttcgtg ggagtatcat ctttttggcg gtattagcgg tctaattgct
    65461 gcaatattat ttcgaaatca gggaccacct aaaaaagtct ggagttggga acaggaagag
    65521 gatgaaaata caccagaaaa tttgtagtat tttttctgta caaaaattgg tttttggtta
    65581 caaatacatt tgttcgaatg ctttaaacat cggggttaag aaaattgaac agccatttta
    65641 gcttctacaa acttgcgtta aaaaccggat gttcctgcgg gtttgtgcaa gttgacttgc
    65701 aggagtgttt cctgtcttgt catcaaccta acgctttata gaatattcaa catggatgac
    65761 ccttccctaa tttcccaata attccaaacg agagaatccc actttttaaa tacttttgcc
    65821 taaccagaaa attatggaat ggaatcccat attataatta atcaactcct tgcctttata
    65881 gttttagctg taattggagc aattgcctcg tggaggaaaa ttattaatga cgaggggaaa
    65941 gatttcctat cacgattaat tattgatgtt actcttccct tattgattgt ttccaccttt
    66001 ctcaaactcg aaagcaaccc ccgacttctt gtaaacggtt tatggctatt tgttctgact
    66061 tttgtaaata ttggtgttct gtatatggca ggaacgttgt ctgccagagc acaaaagtta
    66121 tctccatctg actcaaccgt gcatgttctt cataccatgt ttggaaatat tgtttttctg
    66181 ggttttcctt tatttgatgc cctctttcct aacggggtag gagtttttta tgcttccgtt
    66241 taccagctgg cttccaattt tattacctat acctatggaa cctataagct aagtgcggga
    66301 gttcaaaaat ccaaattggg taattttttt aatataaaca caattgccct gctgctcggc
    66361 atcctgctca tggtgttaaa ggttaaacta cctgctttta tcctagagcc aatttcgagt
    66421 cttggcaaat gtaccagccc gctctccatg gtttatattg gagcgctgct ggccggcatg
    66481 aacttgaaat caacttttaa aactacaagt atttatgttc ttacaatcaa caagttaata
    66541 attgtccctt tactactagg ttttatatac tcttttctca gaaggtcgtt taatttaaac
    66601 ctatcaatag aggcatttgt cgttttggtt atgcaggcag ctatgccttg ccagacgatt
    66661 gttgtagtaa tgtctagacg ttacggcgga agccacgtac tagcaacagg gaacttattt
    66721 atctctacca tctttagcat catctcacta cctttggttt accatttttt ggtaagtatt
    66781 tattcttagt taaatatatt attaaattat aaaacgataa ggtgcatata atgaatcaaa
    66841 tataaaaaat caaaacgata tacatagttt ttgtgcgtag attcacaaat tttcaaatgg
    66901 aataattttt aactattatt accatgagag tagttattca gcgtgttaaa agtgctaagg
    66961 tggaagtaaa caatcatgaa gttggctgca tacacaaggg cttattggtc ctttgtggtt
    67021 ttgaagtgga ggaatcggaa gaggatttac aatggatggc ctcaaaaatt tgcaagctgc
    67081 gaatatttga tgatgaaact gaaaccatga accttagcat taaggatata tccggagaaa
    67141 tactagtggt gagtcagttt acccttcacg ccagcactaa aaagggttgc cgaccatcat
    67201 atgtgcgcgc cgctccatct tcgttggcca tattgtttta caacagattc ttaaatttat
    67261 tggagatcga gctgggctct aaaatcgctg ctggggtttt tggaaaaatg atggaggttt
    67321 cattagtaaa cagtggccct gtaactatag tgatagattc taaaaataaa gaattttgaa
    67381 aatgacattc gaagaggcac agaaggaagt tgactcctgg attagggatc acggcgtgag
    67441 gtactttagc gagctgacaa acaccgtgct tctgatggaa gaagtcggcg agctggcaag
    67501 gattattgcc cgcaaatttg gcgagcagtc ttttaaagat aacgaaagcg ccgatggctt
    67561 atccgacgaa atggcggatg ttctttttgt tttaatttgc ctggccaatc aaacaggggt
    67621 atccctttct gaagcatttg agaaaaatat tgaaaaaaaa acacaaagag actcccagag
    67681 gcacaaaaat aaccccaagt tattttaact ttgtagcata accgaacttt taatatttta
    67741 tacagtatgg atttttttgt tgaagataaa tttaaccttg aggttaatgg ggaagaagtc
    67801 gggcgccatt tggcacgaat taaggatctc gcactttccc ggcttggtga taccgataag
    67861 ttgaaggctt gttttggctc gattgaccta acaaccttaa acacaacaga cactgctgaa
    67921 agagggaaac aatttgctga aaaagttaac ggcttcactg ttgattttcc tgaaatgcca
    67981 agcgtggctg caatttgcgt atatccaaca ctagtatcat ccgtaaagga gaacctaaaa
    68041 gctaaagggg ttaagattgc ctccgttgcc gcaggatttc cttcgtcgca aacctttttg
    68101 gagattaaac tttctgaatg taggatggca attggccagg gggctgacga gattgatgtg
    68161 gttattccgg tgggatcttt tctacaggga gatctgtata gcgttttcag cgaggtaaaa
    68221 caaataaagg aaactgctgg cgatattcat gttaaggtaa tactggaaac gggagccctt
    68281 ccttcgctga aagaaataaa gctggcttct tttttagcta tggaggctgg agccgatttc
    68341 ataaaaacat ctacaggaaa gcttgaaccc gcagctacac ccgaggccgt ttttgttatg
    68401 tgtaccgcaa ttaaagagta ctttgaaaaa acaaaccgta aagttggcat taagcctgcc
    68461 ggcggcgttg taacttcttc cgaagccctg gtttactata ccatcgttga gcaggttttg
    68521 ggttcgtctt ggctatcccc cgacctgttc aggctgggag caagcagact ggcaaacaac
    68581 cttctttcag atatttatgg tcgtgctgta agctactttt aagtctcaga acatacagaa
    68641 caagtttttt ttatataatt gaagaaagcg cttatttagc caaaggttgc tttatccctg
    68701 ggctaggctc ctgaaaacct ctttttacca taagggcgac ggatatacgc cattaagcgt
    68761 tagcttcctg agctgatcaa ccacatgaat tttatcgcca ggataggtaa ccccaaacca
    68821 ttcggaagta gtagaaagaa ccttacatgt tgccaatcca tcctgaatgg cacggttgac
    68881 aactgttggt atatatattt ccgatttttt gtgatcgggg atcccgttta aaaactcttt
    68941 aagcaatgtt tctgtaaggg taaaaaagtc ctgagtaaac ccccagaaat tcatcgatac
    69001 cagcatgctg ccgtctagct cgatgcggtt acccgagtcg tctatattaa atattttccc
    69061 atcaaccctt tgaatcctgg taaactcaac aatactctta agaaatgatg cgttgtcaac
    69121 ttcacaagcg cccctagaca cagacccttg ttcagagagt gtattgttga gcctgaatcc
    69181 caccataaag tattggccgt ttttaagatt gcttcttgag agctcgtttg ccagagtttt
    69241 aaatgtatcg taaccataaa aatcatcagc atttataacg gcaaaaggct cgttaaccat
    69301 tttacgggct acccaaacag catgagctgt accccagggt ttttcccttt ccggaggaca
    69361 ggtaatgtta tcgggaagca tgtcaagttc ctgaaagact atatcaaaat taacatcccg
    69421 gctaaacctg tttccaaaaa ttttcataaa atcttcctcg atactgcggc ggataacaaa
    69481 aataactttt ccaaaaccag cccgaatggc atcgtaaacc gagtaatcaa ttatggtttc
    69541 gccgtatggc cccagaccat caacctgctt taaaccacca tatcggctgc ccattccggc
    69601 agccaggata attagcgttg gcttcatcat ggtatgcttt acaaacttaa atttttaggg
    69661 ttgacaaaag tagcatttat ggcacagcta tggtaaacca accattcgaa ttttgatatt
    69721 ttttgcaata taatttgata ataataacga tatagtccat atgtatagcc acatagaatc
    69781 agcaattaaa ctaaaagttt aaacattgaa tttctttcta tacaaatagc ttaaaggtat
    69841 taacagttaa aatacctact tttgcagaaa caagggctta atgatgagac gttcggatat
    69901 agaagttatg gctcctgcgg gaaactttga atcgctgatg gctgcaattc aagggggtgc
    69961 cgatgcagtt tacttcggag tcggtaaatt aaatatgcgg gctaaatcca gcgtcaactt
    70021 tcttgaagac gacctttacg aaattgttcg catttgctca gcaggcaatg ttaaaaccta
    70081 cctgaccgta aataccgtgc tttatgatga ggacatagac gacatgcata ggttaattga
    70141 cactgccaaa aaggccaggg tttccgcggt aattgtttca gaccaggccg caatggagta
    70201 tgcccgtcag gtaggagtcg aagttcatct ttcaacgcag ctaaatataa gcaataccca
    70261 ctcgttaagg ttttactcgc agtgggccga tgtggtagtg cttgccaggg agctcaacct
    70321 tcagcaggtc aaattaattt cggaaggaat tcataagcat aacattaatg gtccctcggg
    70381 gaacaaggtt aggcttgaac tttttgttca tggagcactt tgtatggcca tctccgggaa
    70441 gtgctacctt agcctccacc aaaagaatta ttctgccaat cgaggcgaat gcctgcaaat
    70501 ttgcaggaga agctatacgg taaccgataa cgaaaccggc tcgcagctcg aaatagacaa
    70561 cgaatacatc atgtcaccta aagacctctg cactatttcc tttctcgatc aaattcttga
    70621 tgcgggcatt agggtgctta aaattgaagg acgcgctagg ggtcccgagt atgtaaaaac
    70681 cgtaacatcc tgctatgccg aggcagttga agcaattgtt tcaggccaat atacacccga
    70741 caaggttgaa ggttggacta aacggcttac atcggtattc aataggggct tttgggatgg
    70801 ttactacctt gggcgcaggt tgggggagtg gagcaattcc tatggaaata tcgcaacgga
    70861 gcgcaaacag tatgtgggaa aaattagcaa ctacttttcc gacatgggag ttgccgagct
    70921 aaacgttgag gcaacggagc tataccttaa tgataaggtt ttaataatag ggcctactac
    70981 cggcgttatt gaaacactgg tatccgaaat acgtgttgat aaccagcagg ccagctttgc
    71041 tccaaaggga cttccgtgct ctattcctgt cccatcaaag gttcgtcgct ccgataagct
    71101 ttacaggatt atttattcta agccttgatt gcagcctata ccacccaagc gttaacttag
    71161 gaatgggtag aatgttttaa gtgatatttt cagaaacatc aatacatgat tgataaacaa
    71221 aattataaat gctcaagttg aaatttttat tttagatgat ggtttaaaga tttttacaag
    71281 gatttcggca cattgcttaa atcggtgatt gtaataacca caaaagatct ttttgaattg
    71341 ttaatcattg aatttattta aatatatcat taagtaactt aatataaatt aactatggtt
    71401 aagaatttta gcgagttgtg caataacatt tttgatgaat gcacaaaata ctaccacact
    71461 atggataatg tggatgctaa cccaaacgtt ccttataaaa gtaacaccat cgagcactcc
    71521 ctcttcatta agaattggat tgatgcagtt cagtggcacc tggaagattt gattcgcgac
    71581 ccgaacatca atcccgaaaa agcgcttctg ctaaaacgaa gaatcgacaa gtcaaatcaa
    71641 gatagaaccg atttggttga gcaaattgac agctactttt tagaatactt taaggatgtt
    71701 cgcccccaaa atggggctac aatcaatacc gaaagcccgg catgggcagt tgacaggctc
    71761 tccattctag ccctaaaaat ttaccacatg gaacaggagg ttagcagaac taatgcttca
    71821 gctgatcatg tggctacttg ccgacaaaaa ttaactattt tactggagca acgaaaagat
    71881 ctctccatgg caatagatca gttgttggac gacattgcca atggcagaaa gtatatgaag
    71941 gtatacaggc aaatgaaaat gtataacgat ccctctctga accccattct ttatggcaag
    72001 aatgaaacca acccttaagg agaacaaacc gaagaaactt ttggtaatcc gcctcagcgc
    72061 cattggtgat gttgccatga ccgttcctgt tctctggtcg gtagtaaagc acaaccctga
    72121 cgttcgcgtt acttttgtat cgcagagctt tgctcgtgac atagttagcc aaattcctgg
    72181 aattcaattt tatgaagccg acaccaagaa tcgccataag ggtttttggg ggatttttaa
    72241 acttttcagg gaacttaaaa atcttgatag gtttgatgct gtagccgatt tgcacaatgt
    72301 aattagaagc cgggttattg gcgttttgtt taggattggt ggaatacgct atgaggtaat
    72361 agacaaggga agaaaggaga aaaaacaact atgtagcgta aagaataaaa aattggtaga
    72421 gttgaagcct actaccgaac gctatgcaga tgtttttcgt cacttgaacc tggtcgttag
    72481 tagcgatttc gattacttat ttgacgaggc ccggttgcct gatgatatta gtgcatacct
    72541 ggggtataaa agtggcacct ggataggtgt agctcctttt gcaaagcata aagggaagat
    72601 atatccgctc aacctgatgg aagccgttgt gtcgagattg gcagcaaata agagttataa
    72661 gatagtgctt attggtggta gaaattttga aaaggagttg ctggaggagt gggcaaaaaa
    72721 atacacaaat acattttcag ttgccggtaa atacagtatt ttaaacgagc ttaaaattat
    72781 ttctaatttg gacgtgctta tatccatgga ctctgccaat atgcaccttg cttcgcttgt
    72841 taacactccg gttgtgagca tttggggcgc cacccatcgt tttgcgggat tttacgggtg
    72901 gaagcagaac gccgataaca ccgtagaagt tgagttgtcc tgtcgccctt gctcggtgta
    72961 tgggaacaag ccttgtttcc gtaaggacta cgcatgttta aaccgaatca cccccgaaat
    73021 gattattagt aaggtaaatt cagtggttga tagatatgga agaatttgaa aaatccatga
    73081 tttagcctaa agttgacagc tgaatgggtt tatcgttaaa tttaatttta tactgttgaa
    73141 tgataaagag atatataact ttaattaata taaatgtgaa gaaaaatatt gattatctga
    73201 actcaaacat catataaaaa tgttaatatt acaggttatg agcatattat aatgtaatta
    73261 tgatttatag cactccgttg tctttctgat tagtttaccc atcaccacca tggttggtat
    73321 tgtttaaaag tgctaatatt ttgtgaaaac aatttcagac gggatgctca tataatcgaa
    73381 ctaacggagg gattacggtt cgagggttac cacaaaaaaa taaaatttct gaatggatgg
    73441 atagcgcact aagtggctca accagcgatt aacccaacac ctttattacc cgttctttaa
    73501 atgtaaatat tggagataag cttttcaaac actgtagtaa atatttaatc ataaatcaca
    73561 acaaccagaa aataacccga ataaaaatat tttaattttt acttaagcgg gcaagtttta
    73621 atgccacagc cacttgcatg gtgtttttta cttacctttg cagcaaaata aatattgtat
    73681 gtcgtacgtt acgaaagaaa agttgtttag taaggcttgg tttaaagcct acaccctaat
    73741 aacaattggt gcatttgtgt tagctgctgg gttcgtgcta tttatcgatc cccatagaat
    73801 tgcacctgga ggtgtttacg gaatcggaat aattgtacac taccttaccc agggcgtgtt
    73861 ttcctgggcg cccgaaggat ttccgattgg gacggtaggc ctgctactca acattccgct
    73921 taccatttta ggaattaaaa ttcttgggcc acgttttggt gttaaaacgg tggtgggctt
    73981 tattttgtcg tcggttttta tagataccct tcacatgatt tatggctacc atcctcttgt
    74041 ggacgatatg ctactttcgt caatttttgg aggggttcta attggcttcg ggcttgggct
    74101 gatttttaaa tcaaaggcaa ccagtggcgg ttccgacatt attgctatga taattgcaaa
    74161 gtacaccagg ttaccattag gacagctgat gatttatgtt gactctacca tcgttctaat
    74221 agcacttgta gcatttaagg attggaaaat ccccctctac tcatgggtgg tgatctacat
    74281 aacgggtaag gtgatagata tgaccataga gggagccagc tacgataaag ccctgtttat
    74341 cattagcgac cgttacgagg aggtgaggtc aaaaatcatc aacgatctcg aaagaggcgg
    74401 aacgcttatt agcgttaagg gaatgtatag cggtgaggaa aaaaagttga ttttcacgaa
    74461 cgtttcccgc agagaggttt ttatgcttaa ggattacatt cgtgaaatag atcctaaggc
    74521 ttttattaca gttattgatg ccaacgagat tcttggcgag gggtttaaac caatcagcga
    74581 agctctttag ttctgcaggc aaatgttaaa caattcaacc agttggttta taatccatca
    74641 cataatttaa aggtcagcaa cagtgaatca attactttaa atgaagaatg gctacagaat
    74701 aagccctgtt attgtgctgt gaaataaact ttatatcttt gaagcctcaa attctttgaa
    74761 acggtattgc ccgggtggcg gaattggtag acgcgctggt ctcaaacacc agtggtagca
//
LOCUS       DAOPHX010000002        55230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_503_length_55230_cov_6.515777, whole genome shotgun sequence.
ACCESSION   DAOPHX010000002 DAOPHX010000000
VERSION     DAOPHX010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 55230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55230
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_503_length_55230_cov_6.515777"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..981)
                     /locus_tag="PK502_00300"
     CDS             complement(31..981)
                     /locus_tag="PK502_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPG99288.1"
                     /translation="MQKQMDDVLWFNRVGDVAFVDKVYIYGPPPANVKNPTAMGATNP
                     VKFWAYVFIPKYIDPSKKYPLLVFPHGGVHADFTTYHTHIIREMMAQGYIVVAAEYRG
                     STGYGRSFWENIDYGGLENEDVYASRNYMVENYDFVDEKRVGIIGWSHGGMITLHNIF
                     DHPNDYACAFAGVPVSDLIMRFGYSTDDYRELYSAPYHIDKSPREDIAEYKRRSPVWQ
                     VDRFKNTPLLIHTNTNDDDVYMIEVEHLIQALKAEGKKFEYEIFKDMPGGHSFDRIDT
                     KAAREIRVKIYKFLARYLTPPNPIRSVDDINRAAYLLHNP"
     gene            complement(1220..2476)
                     /locus_tag="PK502_00305"
     CDS             complement(1220..2476)
                     /locus_tag="PK502_00305"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG99289.1"
                     /translation="MTFHLNPASYLSKIKFAFRALKSPNFRIFFYGQAVSLMGTFIQG
                     LALGWLVYRLTNSPFLLGAVSFAGQIPSLILMPFAGVYADRLNRRKVLVITQGISMIV
                     ALTLATLYFTHTIQVWQIFVTSILNGIAIAFDAPFRHSFMLEMVGDKDLLQNAIALNS
                     TLVNSARFIGPAIGGVLVAWIGEGFCFLVNGISFMAVIASLLHIRVAQVERKPHNSSI
                     FKELKEGLSYSYNFLPIRYVLSLLVVVGFLGFPFQTFMPVFARDILHGNSQMLGLLTG
                     AVGAGALTGAILLASLKSIKVIPNIILYSSLTFGTSLAAFSLSSYATLSVGLLFFTGF
                     GMIVQLAATNTLLQYIVDDDKRGRILSLYSLSFNGFPPLGSLLLGAISPSVGIQPTMA
                     VAGGLCAISALLFSRRVPVVKSVVPD"
     gene            2721..3185
                     /locus_tag="PK502_00310"
     CDS             2721..3185
                     /locus_tag="PK502_00310"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HPG99290.1"
                     /translation="MKKVKTIALVAHDHRKRDLVEWVEWNWQELLKHKLICTGTTGRL
                     IEESLQKKKSDVADHIQLTRLKSGPLGGDQQLGALIAEGKIDILIFFWDPMEAQPHDV
                     DVKALLRIATVYNIPTATNRSTADFLISSPLIDEDYNPILFDYSHYMNRKVF"
     gene            3583..5259
                     /locus_tag="PK502_00315"
     CDS             3583..5259
                     /locus_tag="PK502_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99291.1"
                     /translation="MNHNKGKWCRIGSMVLLTALLTGCYDFSNFENFKVEPVNPSIVA
                     PVVNSTITFKELAETEDANTIVIVKPGDTRFYLSFRDTMEIANTSGEFSIPPFAYSKT
                     YQPDAGEIPPVAPPAGESITFSPKTFTQTWQPDADSELKLIKLSQGSMNVTVTNNFGN
                     IISGSITLNFLLTPQNQPYTINIPNINPASSQAISLTNLAGYSFNLFDGTSYNTLTFT
                     ATITLTSMGNSINPGDNINIDISANNLDFTEIYGKVNKTINLSDQSFPLDIFRSTYIA
                     DQHLAEPSLMLKFANSYGIPLSLNVNQLVASNDQTGSMNVVNETVPPDGSLLIGAPNS
                     LNYVGSPSQEFAMDSLKLDYQNSNIEDVFDFAPNNFTLKSGITLGDASTSHDYFVRKN
                     SNLTLFSNVEVPLVGWVETNGIRDTVEIDLPDIENDLNMEDDDELSLTLKFRFNNGIP
                     LNVYFQAYFIDDYDHELTRLYTNVAEEQLIESAAVNPSTGKVTTPNVKISEISIDRNR
                     YKQMQGATKLVLQVRFKTGGSTHQNIVVESTNSIDIQMSIKAKGTVRFDS"
     gene            5450..6802
                     /locus_tag="PK502_00320"
     CDS             5450..6802
                     /locus_tag="PK502_00320"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HPG99292.1"
                     /translation="MKRLSLLALILTVGIYAKAQSELTLPFMPGVFQSSFVNPTVIPE
                     HTFSMGFSIYTQVVTNGFVPNNVLIFRDDTMHFDPNLLLDDLRDKNLFYMGQNTDIFH
                     IRTKILNGYYWFAIRQKMESSFFYPYDLFRMVIEGNEPYIGQTLDFSNLKIFTSLFNE
                     YSFGMTKEYPHWIFGGRLSLLQGLSNINFNPTRLSIKVEDEMYGQLANADAQLRTSGI
                     PKDGNGDPSFDHVEEPEWITDYFTNFKNKGFALSGGATYRYDDRTRISLSFRDVGFIT
                     WKDSIETYTLNGNTNFTGLDIMSDLLYGNDVEVDSIFENILDEDFHRDTIYNKYTTWL
                     NPKFNLTASYDLARRTTVGFSFSGVVNQKLYTALTLSLSQGIGRFFSIVGTASINQRS
                     FTNLGLGLMLKPGPLQIFVVADNLYAAHNPLTTTNFNIRVGVNLVFGRINQPEGLPFR
                     "
     gene            6846..9212
                     /locus_tag="PK502_00325"
     CDS             6846..9212
                     /locus_tag="PK502_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPG99293.1"
                     /translation="MRVSGIKNKIVALLLGSMVALGLQAQTSFTPINLGDRVNSEYPE
                     INPILHPDGKTLYFSRANNPENTWGGVNSQDIWYSVLGDDGTWSQAQRLPRAVNIGRY
                     NALFAILNNGKTFLINGHYSPNGKYWIERGLSTVEQVENENWGKPQSIEVKGYSNKNR
                     GKTTTIFVSPDEQKIFMAFSKKVNGKKASIYLSERKGDRRYSKPKKINFLGNNKCESM
                     EAPFLTSDGNALYYSCKINGDNNLYMSHRTDDTYRSWSEPIPLNDTINTPGWECYYHL
                     NSKGSWAYFCSNTNSLGKSDIFRVKIFEENPFVRLHGLVLNKADQKLMLADTSYILKV
                     NGSEPEKLKVDRAAASYELLLPFGQSYQLQPEMKNWIGIPELYDATGIKEFTDVELNL
                     YFEPIPYVKIFGKVINTRTNLPISPEIGFKVLINGLENDSVKYIRDISGYQALLPLGQ
                     KYVVSLNLPNYRSEADTVDVSNISFYTEKQVDFHVTSAPWVEVYGIAMDNVSFTPIPG
                     SSSPKLLVNGYPADSIAIDPFTGEFTIKLPYGIKHNLAISSKDYNPLDNVVDLTGYVE
                     FTRINHDVYAERKDANMATLYGKVINLKTGQPVESSIPVKLKVNGVETRAFIYDSTTA
                     AYTLKLPVGVSYDILPSVKNFYNKYEQVDLTKTKKGTKVPKNFYITPIEVGQAVNIDF
                     IYFETGKSTLKPQSFRSLNALVDFLNEYPNVIVEIGGHTDNTGSAAINEKISNLRAKA
                     VADYVVSMGIPASRITSKGYSFNKPKASNATATGRAQNRRVEFTIVGI"
     gene            9417..10040
                     /locus_tag="PK502_00330"
     CDS             9417..10040
                     /locus_tag="PK502_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HPG99294.1"
                     /translation="MFALKLKLLLSCFVAVLALVNPIQKVFVMTSLQDQFSEADTKMV
                     ALKSSLTALLILLFFLLLGEVVFNYVFHVELYAFQITCGVVLFYNGITGLQKGIFIKV
                     DQNISIHDITAVPIAIPMIAGPATITAAVTFPAHYGHFNTIVSIVAALAINLLFMLYA
                     KHIARFLNRFNLMNPLVRIFGLIVATIGVQMTLNGIWGFVVSVAGHV"
     gene            10112..10933
                     /locus_tag="PK502_00335"
     CDS             10112..10933
                     /locus_tag="PK502_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPG99295.1"
                     /translation="MEQKNFIDIDQVIASKSPTLQKSLPRFIVNYLKRIVHQDEINEI
                     LAKHGHKQGAEFVEAVLSTMGVNYSVEGLENLQDNQRYLFASNHPLGGLDGMVLIHLL
                     AKRFDEVKFPVNDLLMHIKPLSSVFIPVNKLGTQSASNAIQLEEAYSSSAQILYFPAG
                     LCSRKQKGRIEDLEWKKNFIVKAIKHERDVVPVFFDGRNSTFFYNLARLRAKLRIKTN
                     IEMLYLSDEMFRQKGKSITVKIGKPIPFSTFDNTRNAHEWAQWVKHVVYQMKPAS"
     gene            10995..11954
                     /locus_tag="PK502_00340"
     CDS             10995..11954
                     /locus_tag="PK502_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG99296.1"
                     /translation="MQEIIEPVDRNLLMAELTPEKFVRNTNNAENELYLFTAHDSPNL
                     MREVGRLREFTFRSAGGGTGEEVDIDEYDICDNCYKQLIVWDPKEREILGGYRFHSVD
                     PSKGDEINLSTKHLFNFSEKFVREYIPKMIELGRSFVQPKYQSLSRGGKGLYALDNLW
                     DGLGALIVLNPHIRYFFGKVTMYRSYNVEARDTLLYFFEKYFKDNEQLVTTIHPIEVN
                     MDRDKMERLFTGKNYTEDYKILSHKIRELGEKIPPLINAYMNLSPSMKVFGTVINPYF
                     GNVEETAILITIPDIYQHKMERRTGTFVRKLAGKYLRPYRKES"
     gene            complement(12039..13940)
                     /locus_tag="PK502_00345"
     CDS             complement(12039..13940)
                     /locus_tag="PK502_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPG99297.1"
                     /translation="MSEQIKHECGIALIRLLKPMEYYHLKYGTWQYGLQKLYLLMEKQ
                     HNRGQDGAGLTSLKFDLEPGKRYIDRERSNSDSSIKDIFDAVNRSINQYGHRPELAGD
                     SAWAKENIPFAGEIYLGHLRYGTFGNNSIDYVHPVMRDNNWKSRTLVLAGNFNMTNVD
                     ELFNKLVSLGQHPRDYSDTVTVLEKIGHFLDEENQQVFRQYKNEGYSNMEISKLIAEN
                     INIGKILQAATKDFDGGYAIAGLIGHGDAFVIRDPWGIRPAFYYLNDEFAVVASERSA
                     IQTVMNVATDDVKEVNPGYGLIIKRNGTITHEEIRVPQQRRSCSFERIYFSRGSDRDI
                     YQERKKLGELLTPAILKAINYDHSNTVFSYIPNTAETAFYGMVKGVEDYLKVEKKRMI
                     LEKSSSLTESDLDEVIAIRLRVEKIAVKDVKLRTFISQDKGREDLVGHVYDITYGAIR
                     PGIDSLVVIDDSIVRGTTLKESILRILDRLNPVSVVVVSSSPQIRYPDCYGIDMAKLS
                     DFVAFKAAVGLLHDTGQEAIINKVYRKCKEQQYLPKEEIVNYVKEIYSPFTDDQLAEK
                     IAQLLTPKDMRAKVKIVYQSIENLHLACPNDLGDWYFTGNYPTPGGNRVVNNSFINFI
                     EGRNNRAYQ"
     gene            complement(13986..14990)
                     /locus_tag="PK502_00350"
     CDS             complement(13986..14990)
                     /locus_tag="PK502_00350"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPG99298.1"
                     /translation="MSYTLTTYLYKMEQFRNKGDKATRTGFGEGVVDIGELNPNVVVL
                     GADITASVGLDGFKRRFPERFFSFGIAEQNITAVAAGLALAGKLPVFSSYGVFAALRA
                     ADQLRISICYNNVKVIIGGAHAGISVGPDGATHQALEDIAYLRALPNMTLISPCDANQ
                     ARAATIAAMQQVNGPVYIRFGREPVPNFTDEDQKLVVGKGQVLMNGDDVAIVATGHEV
                     WESIEAAHILAGQGVNAMVVNIHTIKPLDIPLLVDIAHHTGFVVTVEVHQVNGGLGGA
                     VAEVLGKYCPTPMEIVGMPDCFGESGTPAELLQKYGLNSQSIASKVLETLSRNKKGIL
                     "
     gene            complement(14938..15783)
                     /locus_tag="PK502_00355"
     CDS             complement(14938..15783)
                     /locus_tag="PK502_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPG99299.1"
                     /translation="MHQLLKVSKQLREDVVRSLASAGSGHLGASLGLADIFTVLYWQV
                     LHHRPLQPGWSERDRLILSIGHAAPVLYAALANSGYFPREELLTLRKLGSRLQGHPGR
                     DHGLPGLELSAGSLGQGLSVAVGLALAAKIDRAPWRVFSIHGDGELQEGSIWEAAMSA
                     AHHKLNNLIALVDRNDCQIDGRTSEVMGLEPLRAKWESFGWRVLECDGNDIAQLVETF
                     EVAEHGDVKPTVIIARTLMGYGVKSIQDDYRWHGKAPSPTEAEKFLDELHAHYLSLQN
                     GTVQE"
     gene            complement(15803..16264)
                     /locus_tag="PK502_00360"
     CDS             complement(15803..16264)
                     /locus_tag="PK502_00360"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPG99300.1"
                     /translation="MRSLPALLFNSLFLICVFAAEHVHGQGVSGIVQNQHEGNEQGKV
                     KITQDPVVSNLLELHILQNAKNPGMNGFRIRIFLELGQNARRNSEAVMNAFMEKYPDI
                     RVYQSYDNPYWKISVGDFRTRESAQKLYHQLLVDYPKAFIIPQWINFPPLE"
     gene            complement(16303..16830)
                     /locus_tag="PK502_00365"
     CDS             complement(16303..16830)
                     /locus_tag="PK502_00365"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HPG99301.1"
                     /translation="MKVKRFLAAFVPIFMVLMSGCAPKFQDIKINGINKVEYNGLKND
                     VVKLNLAVGIENPNSKRITVKEVEFKSWIKQRELGTVRVKNRIEIPPNSSQIYSIPVE
                     VKLNSSADALKLMISGEKILTMITVEGFVKGSSFPIVKKIKIPRQPLNNLINKYQNGF
                     IITDTISVRNSTVGN"
     gene            complement(16950..17696)
                     /locus_tag="PK502_00370"
     CDS             complement(16950..17696)
                     /locus_tag="PK502_00370"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPG99302.1"
                     /translation="MSGHNKWSTIKRKKGALDAKRSKEFSRIIKEITVAVKESGSDPD
                     GNPRLRLAINNAKGVNMPKDNIMRAISKAEKDPENFHELTFEGYGPGGIALFIECLTD
                     NNNRTVSAIRSILTKRGGSLGTNGSLSFLFDRKGIFTIAKDKLKLDEVELDLIDAGAQ
                     SIDDNGDFYVVTTEMEDFGRMNKKLEELNVEVENASLQRIPNDYKTLDKDSSLRFLKL
                     IDELEDNDDVQNVYHNLEMTDEVVAAMENE"
     gene            17875..19275
                     /gene="miaB"
                     /locus_tag="PK502_00375"
     CDS             17875..19275
                     /gene="miaB"
                     /locus_tag="PK502_00375"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPG99303.1"
                     /translation="MDFNGEEPNIFEGISTAGTGNGAKVYLETYGCQMNVNDSEVVAS
                     ILLANGYSITQEINEADIILINTCSIRENAETRVFGRIDLFGQVKKNKPSVIIGVLGC
                     MAERLKDELLEKKKIVDLVVGPDAYRELPTLLRAAESGQKGVNIMLSADETYADISPV
                     RLDKYGVSAFVSIMRGCNNMCSYCVVPYTRGAERSRDPQTIIHEVEELVRNGYREVTL
                     LGQNVNSYSWSESKGMVKHTVNFADLLEMVALVSPFLRVRFSTSHPKDLTNDVLYTMA
                     MYENICNHIHLPVQSGSDRILQLMNRKYSRNSYLNRIQAVREIIPDCAISTDIIAGFC
                     TETELDHDLTLSLMREVGFDFAYMFKYSQRPNTKAARHLVDDVPDEIKSRRLTEIIEL
                     QGKLSEESKKKDVGKRFEVLVEGTSKKSDDFYVGRTTHNKVVVFPKEASSIGDFVLVE
                     VTSCTPATLIGKIVDE"
     gene            complement(19330..20100)
                     /locus_tag="PK502_00380"
     CDS             complement(19330..20100)
                     /locus_tag="PK502_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99304.1"
                     /translation="MSKINLTPGEINDLIDLYQTEIDRTQRRIENLKSILKKIDRAQE
                     EVSEPASRVAAPARRGRKKLVESVPAAGEAVTPEAKVDGRKVRKGRNAKAVAKSAAPK
                     VKAIRKTRKSKANAATSEETKLAATKRGRKANLKSKVAAKKSPRKESVVRKKIKSGAG
                     PDKVKWIDLIQDILRSKQNLMLANSLTVEAMERLGIDESDRDRVRMAVATNLTRLTKN
                     LKLVVKFKPEGSTNYFYGLTEWFDEGGNLKSEFQNKLM"
     gene            21522..24803
                     /locus_tag="PK502_00385"
     CDS             21522..24803
                     /locus_tag="PK502_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPG99305.1"
                     /translation="MKRKSFKTAMLLVALGSSLWAQNSSNPLWMRYPAISPDGKHIAF
                     SYMGNLFRVSSDGGMAELLTSNAAYDFSPVWSPDGQTIAFASNRYGNFDVFTIPAMGG
                     EAMRLTFHSADEIPSAFSPDGKSILFSACIQDNANNIQFPSGALSELYSVPTQGGRIA
                     QILTTPAEQAVYNPEGTEIAYTDIKGYEDKWRKHHKSSVTRDIWIYDISTGKHRKVTT
                     FEGEDRNPAFAPDGKSLFYLSEMQGNSNVFSVSLSSIGQPESVSSFTNHPVRFLSVAR
                     NGMLCYQYNGELYTQKPGSSPQKVNIQIATDQTETRVKYENLSTGITEMNVSPDGKEV
                     AFIIRGDIFVTATDYPTTKRITNTPDQERSVSFSADGRKLLYSSERDGSWKIYESSIA
                     SAEEPNFSLSTIIKEQVIVDIPEEAFQPLYSPDGKEVAFLKERTTLCVVNLETKKIRT
                     VLDGKYNYSYSDGDQWFQWAPDGNWLAVNFMETPSWPDSDIALVKADGSGEFHNITQS
                     GYDDRNPKWTMDGKAIIWLNDKMGMRSHGSWGSQFDVYSAFLTREAYDEFKLSKQEYE
                     LLKEKRSKDKKDNGAGEDKESSSDKKKEVKAKKDKEAQRKPLIIDYENLDDRKVRLTI
                     NSSDLSDAILTDDGEKLYYLSKFEKGYDLWVNNLKDNETKLLVKIDGFAGDLQIDKDK
                     KFLFVNSNGTPMKIEIATNKLSPIKFNAELETNYPRERDYMFEHMWRQVEKKFYDPNL
                     HGVDWKFYKSEYARFLPHINNNFDFAEMMSELLGELNASHTGCRYRPKPENADQTAAL
                     GVFLDYNFDGKGVKIIEITDNSPLVKANSKVKPGCIVEKIDGIAIECNSDYYKMLNHK
                     ANKLTLLSFYNPQNGERWEESVKPISRSIESELIYKRWVRKNNATVEKISNGRIGYVH
                     VRGMDSQSFREVYSDILGKHRNKEAIIVDTRFNGGGWLHDDLATLLSGKRYVDFVPRG
                     QLIGSEPISKWYKPSVVIISEGNYSDAHGFPYVYKTLGVGKLVGMPIAGTMTAVWWES
                     QVDPTLVFGIPQMGVKDLNGKYLENQQLNPDIKVAISPEEAQSGVDSQLVKAVEELLL
                     ELNQK"
     gene            24890..25075
                     /locus_tag="PK502_00390"
     CDS             24890..25075
                     /locus_tag="PK502_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99306.1"
                     /translation="MKKIALFVIAAGMISMVACKSAPKQEEATEQPATEQTQQETAQP
                     DSAAQAAETPAQPAPAQ"
     gene            complement(25162..26910)
                     /gene="aspS"
                     /locus_tag="PK502_00395"
     CDS             complement(25162..26910)
                     /gene="aspS"
                     /locus_tag="PK502_00395"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPG99307.1"
                     /translation="MLRTHTCGELRISDVQKVVTICGWVQRIRNLGGMTFIDIRDRYG
                     VTQVVIDEKSSNELHSIVEQLGREFVVQVRGEVVERTSKNPKNPTGEVELRTSNITIL
                     NQSELPPFTIEDDTDGGDELRMRYRYLDLRRNPVKNALMLRHTMAQEVRRYLCNKGFL
                     EVETPFLIKSTPEGARDFVVPSRLHHGQFYALPQSPQTFKQLLMVAGFDRYFQLVRCF
                     RDEDLRADRQPEFTQIDCEMSFVERDDILDVFEGMARHLFHTVKGVEFDAPFRRMTYH
                     EAMSLYGTDKPDLRFGMSFKDIGAQLKGKGFSVVDNAEYVGGICARGCASYTRKQLDE
                     LTDFVKRPQVGASGLVYIRYNDDGTLKSSVDKFYSADDLMLVAKVFEAKPGDLMLILA
                     GKRNKTLPALSELRLEMGNRLGLRPKNQFAPLWVVDFPLLEWDEELQRFFAMHHPFTS
                     PHPDDIPLFQSNPAAIRANAYDMVINGVEVGGGSIRISNRELQQTMFNTLGFSDDEAE
                     KQFGFLLNAFRYGAPPHGGIAFGFDRLCSLFGGSDSIRDYIAFPKNNAGRDVMIDSPS
                     SISQQQLDELNIIIKE"
     gene            27011..27451
                     /locus_tag="PK502_00400"
     CDS             27011..27451
                     /locus_tag="PK502_00400"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPG99308.1"
                     /translation="MNLYSHEYFMNEALKEAKVALELKEVPIGAVVVCNNRIISRAHN
                     LTETLNDPTAHAEMQAITAATTYLNGKYLDQCSLYVTLEPCPMCAGAMFWAQLGKLVY
                     GASDPKRGYSLHGNSILHPKTLVVGSILTNECSGILVDFFRGRR"
     gene            27717..28562
                     /locus_tag="PK502_00405"
     CDS             27717..28562
                     /locus_tag="PK502_00405"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HPG99309.1"
                     /translation="MYQPAQPKIVFSDIDGTLLNGSRQLSATTIQTIRKITAAYDVYF
                     ILVSARMPMAMQAIYDELCLKTPIICYNGALIMESLAGGFDNQREICSHSIDPRIPLS
                     IFNHTLEHNLHFGLYSNNSWFVSKNDVWTEREENNTCVKAVIANNMPGIISQLASAGE
                     PIHKLMVMGSPVEIDLLINSISLESRNKITIYRSKETFLEISPAQSSKAKACSLLLDY
                     LNLKPEQAVAFGDNFNDMEMLRIVGTGVAMGNASEEVKRHADKIAPPNTEDGVAFVLA
                     EFFGI"
     gene            28559..30382
                     /locus_tag="PK502_00410"
     CDS             28559..30382
                     /locus_tag="PK502_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPG99310.1"
                     /translation="MTSLELLSPAKDLKTGMAAITHGADAVYIAAYRFGAREKAGNSI
                     EDIGKLTEFAHQYFARVYVTVNTIIFDNELDEVQNLINELYRVGVDAIIVQDLAILSM
                     NIPPIALHASTQMHTNTLAKLKFLEQCGFQRVVLPRELTLSEIGSYCGSTSMEIEAFI
                     HGALCVSYSGQCYMSQRITGRSANRGECAQPCRSSYDLVDEHGKILVRNKHLLSLKDL
                     NLTSQIEQLVDAGVTSFKIEGRLKDITYVKNITAHYNKILNSIISRRPEFRRASSGVC
                     ECSFDADPEKTFNRGYTQHFINGRTVNQTSFHTQKSVGKPIGIVARVNADYFEINSYE
                     AVHPGDGLCYFTTIGQLSGMLVNRVMGNRIYPRNNPTGLSEGDTIYRNSDIEFEKQLN
                     GKSARRWVGVRIAVSDNNGNLKFSATDSDGIAVEYTLNNNFDEAKDKARSVEMLIRQL
                     SKSGDSIFRVDGVAVQDYTTPKFIPISTVNEARRMLLNNLILERLKQHTRPFRGKYFP
                     AVLYPEQAITYKGNISNTASQDFLLKHGVTSIVEAVEISAPKGVFELMVTRYCIKFEL
                     GLCPSKQKASPTGQLYLQNSSTLYPLVFDCNECQMKVMSPK"
     gene            30682..31584
                     /locus_tag="PK502_00415"
     CDS             30682..31584
                     /locus_tag="PK502_00415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPG99311.1"
                     /translation="MIQLLTPESWPEYELIDSGDGEKLERIGSQMVARPEPQALWRKQ
                     LSNEEWVKLADAYFVKSKKTKPTENEWGEWRLKSGAKEQWVVSYNQSGLAFRLRLGLT
                     SFKHVGIFPEQAVSWNYIYNTIKQLDLVETKVLNLFAYTGASSLAARAAEAKVTHVDS
                     VKQVVSWANENMLLSGMDNIRWIVDDALKFVSREVRRGNLYNGIILDPPAYGRGPDGE
                     KWTVEENLYQLLELCKQVMEPKNNFLVLNLYSMGFSPLIAENLIRDIFGVPSYEVGEL
                     FVSDRAGRKLPLGIFVRFRSGSNF"
     gene            31740..32120
                     /locus_tag="PK502_00420"
     CDS             31740..32120
                     /locus_tag="PK502_00420"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPG99312.1"
                     /translation="MEAIKIQGTSQYPTIVLDSEAGRFEFSGNSLPEDPKAFYEPILS
                     WLDAYAQNPNPETVVRFKMVYYNTPSSKQIFQIFKKFEKMAASGHKVSVVWAYNEDDI
                     DIKDAGKDLSDHIKLPFRLESYKE"
     gene            complement(32164..33456)
                     /locus_tag="PK502_00425"
     CDS             complement(32164..33456)
                     /locus_tag="PK502_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99313.1"
                     /translation="MRKILLLTAAMVCLAGYTFSQDKVLDKSGKKPKWVNGIEKDYII
                     VAGSGPDLQVAQQNALTSIKESIVTSVAQNVKSTSTIQKEEANYNNNVNIFLEKFAST
                     TTAQSGKVPFLQGISLSKVEDFYWEKLQRPDKSIIYTYHIKYPFPEFELMKLVSDFKM
                     RDNELTRQLEDLLAQVDQVESVEQIEKNMAELASLKEYFMDARVDQCNLGITRYRSLL
                     NSIELVELESSLGELKYYLRLGGRSISTTKKPVVKSECARVTSIVNNVDNVTIKYDYD
                     NCYEDPENNILVKYRFGVADVQKPFYFDVAANKASIFVSDPFNFTAISKDSTNVVSAL
                     LDMTVRSKYDSPFTIDKVVIEWKGIPPVVIDNIGQSFQGKGNHNLKLNVEQPIKIAGT
                     SSAGKTLSLISGYIYYRSNNTGESKTYRIYNHSYTTDW"
     gene            complement(33567..34145)
                     /locus_tag="PK502_00430"
     CDS             complement(33567..34145)
                     /locus_tag="PK502_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99314.1"
                     /translation="MKMKSMIMVAAAFTFAAVLSSCGGSKKAADSSFGKEVSVPCNDD
                     EFHSDAKFFRGTGNGISQDLSTAKSKARLDANTNLARSINTTIKSVSDRYVNERQIGE
                     NSEFEQKFEQMSREVVNQQLNNVEVACNKTMQTKDGKFQVFTALQVSKDEVLNNISNR
                     ISKDQKLQLDYDKMKFEQIFNEEMNKLESERP"
     gene            complement(34218..35192)
                     /locus_tag="PK502_00435"
     CDS             complement(34218..35192)
                     /locus_tag="PK502_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99315.1"
                     /translation="MTRRILILVATILGFTVGLGAQNSQGKTDDLGRIALAAIVPEQA
                     EGIPQSARQQLVNKMEQIAVQNGLGAAAANPRFCIVPMVNVLSKEITPTAPPMQAVNL
                     EVTFYIVDAASQNIFSQTSIQVKGVGQTEDKAYIQALKGVNVKAGQFKGFVEKGKEKI
                     IEYYNSQCDVVLKGAQALAGQKKYEEALYILLSVPDVCMECFDKSMDLSVEIYKQYAN
                     YKCTEYMAGAKAAWANMNVDKAAEYLGKITPDMECYSEAVKLADEITQKQLADGANVW
                     EFKMKQYDDSIEKDKMMIQAGKEVAQSWAYWGAASYFNWDWSWLYVKK"
     gene            complement(35221..36180)
                     /locus_tag="PK502_00440"
     CDS             complement(35221..36180)
                     /locus_tag="PK502_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HPG99316.1"
                     /translation="MKKLLFSSILIFSTLAVFGQAKKPVIMVVPSDQYCISRGYKMVF
                     DNQGMKQTLPDYKTALQTDPDLRLVITKMGQIMADRGFPLKDLEQEMKNLEQERAESS
                     MLTSSSSGSEMAESPIDALKRVAKADIIMDLDFEVKRQGPQKYIVFNLKGLDAYTAKQ
                     VSGVAGAGNPSSAASPELLLEEAVLSHMDNFNAGLMRHFEDMFANGREVKVQIKVWSN
                     WGENLESEFGDNSEELSTIIENWFADNCVQGRFSLSDATENFMKFEQVRIPMMRTDDK
                     GRERAVDTRSFVSDLRKYLNGMGIPSKLYLRGLGEAWLILGEK"
     gene            complement(36201..36722)
                     /locus_tag="PK502_00445"
     CDS             complement(36201..36722)
                     /locus_tag="PK502_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99317.1"
                     /translation="MKGKLTTMLAVVLAWVIVFPANAQTRKERKILYNSQYNYEIEPV
                     GVGQDGTKVFKVWGYGKDVGAAVMNAKEAAVAACIFKGLPGGAGSAPTPPICSQPNAE
                     VTHADYFEKFFEVGGKYLQYIAVTNDGDPSGEDRIKIKKGYKVAIYVQVMYDALRKQL
                     EADGIARRLDSGF"
     gene            complement(36742..38394)
                     /locus_tag="PK502_00450"
     CDS             complement(36742..38394)
                     /locus_tag="PK502_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99318.1"
                     /translation="MKIKLLFVNAMLIMAVLGAKAQNAEQPKKIPSTYDRSSITFLYM
                     KVPGENHVQEVESKIGSIVFSDKFYNNNMETLSFDAPFSRTSTEVVPQEAVKKYLTDQ
                     KVAKSIISLWYNRQEDGSMSMDKIFDRGMFNATDAAYIKAQSTKLGNAVLQDYGSRLV
                     GRSYIMVLDYQNVKTMQEAKINNMHGWQATAVGYLFKINYTEETQNALYDCWIYPEDS
                     PAVKAEKIKKFEQLEIPLEFVTKTSVPIVASQPNPDTQIGRFIKQKSDDVLLQELVQK
                     GYDETIYNLEKSHEDFMVKTTIHQVNPIRVKIGKKEGLKCDHRYFAYEYVYNEKTNTT
                     EPRFRGVIRATSKIVDNRQVATGEMPTSTFYQTAGRKLQTGYLVRQQNDMGLEVLAGF
                     EMGEVGGVYGRADFRLGRFVGIRSLFVFGEVGFQTKEYDILSLATEDVSFTHYGAGIA
                     KGFMLTRNVELRPYVGIGQESATSDAIEAANTGNYTTLYLRLGGNLALNLKHNIQLMG
                     GIGTYSFISNASNDNGDLGVTWDEVFPDRGGVSTLLGVKIMF"
     gene            complement(38604..40022)
                     /locus_tag="PK502_00455"
     CDS             complement(38604..40022)
                     /locus_tag="PK502_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HPG99319.1"
                     /translation="MKRRYLLPIWLILFIFPSLISAQNQKGAKYVFLFIGDGMGIQQV
                     NAAQVYADSVLHDTLGVTFMQFPVTTLATTYAANRYVTCSAAAGTALSTGYKTSIGTL
                     GLNANHTDTLYSIATRFRDNGRKVAILTSVSIDHATPAAFYAHAGSRSSYYQIALDLL
                     KSGYNFYGSGGFLEPNGSKHQGSTVASIYELGTRAGYGFTSSFTGVDSLKMLGVKSIV
                     YSTPNAAPEQTLQYEIDRKTSDISLADLTRKAIEVVDNPKGFFMMVEGGKIDWACHDN
                     DAATAIHEVLALSRAVNVALDFYSKHPSETLIIVTADHETGGMSLGNNANGYDLHLTA
                     LSLQKASADRLQAELYDLWSKNSKATFEDYLGLLEARIGLSENGAFALTADERNTLEA
                     AYKRLVNLNAGESKNAQKNAGLLAGDAISILNRKAGIGWTSHSHTGSPVPVFIMGRGS
                     SQLPGLLDNTDIPKLILKAAEL"
     gene            complement(40476..40563)
                     /locus_tag="PK502_00460"
     tRNA            complement(40476..40563)
                     /locus_tag="PK502_00460"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40527..40529),aa:Ser,seq:gga)
     gene            complement(40635..41675)
                     /locus_tag="PK502_00465"
     CDS             complement(40635..41675)
                     /locus_tag="PK502_00465"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I asparaginase"
                     /protein_id="HPG99320.1"
                     /translation="MKNRSSLSILIIYTGGTIGMKENPHTGALSPFNFDNIEEEVPEL
                     QKFGFNLQTISFDPPIDSSDITPDFWVKLAELISSNYEKYDGFVILHGTDTMSFTASA
                     LSFMLEGLAKPVVLTGSQLPIGMLRTDGKENLISAIEIAAAKRDGLPVVPEVTIFFEN
                     NLYRGNRTTKHNSEHFNAFRSDNYPLLAEAGITIKYNYPFIHYPRANSSLVVHTSMDS
                     SIGVIKLFPGITPQVVASALSAPGVKAVILETYGSGNALSQQWFIEMISKAVANGLTI
                     LNVTQCKAGSVNMNKYENGLILKNAGVASGFDITFEAAVAKLMYLLGKGFVGSALALE
                     FANPIAGEITLY"
     gene            complement(41662..42450)
                     /locus_tag="PK502_00470"
     CDS             complement(41662..42450)
                     /locus_tag="PK502_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPG99321.1"
                     /translation="MLLVDSHSHIFAEDFDYDREVVLDRAAQAGVKYHVLPNIDSTSV
                     PRLLETADRYSQCFPLMGLHPTSVNRNFSKELEVVGSYLSIRKFWGIGEIGIDLYWDK
                     TFVEEQKVAFRHQVQLAKKLGLPVAIHARNSFQEIFSILDEEADSTLSGVFHSFTGTL
                     EDYRHILDYQTFMVGIGGVVTFKNGGVDRVLPHINIEKIVLETDSPYLSPAPARGKRN
                     ESFNLTFIAQRVAGILEMPVHEVASLTSANAIKLFNLPINNEES"
     gene            complement(42549..43820)
                     /locus_tag="PK502_00475"
     CDS             complement(42549..43820)
                     /locus_tag="PK502_00475"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPG99322.1"
                     /translation="MLKRAFFSVILALLAIFFVDYFVGISPVRTSKNSEEYSKDTLLA
                     KPILKYGIPVDSFYIEKGSLRYGQTIGNLLLDLNVDPEVVNSLTLVSADSFDPHKFKA
                     GNHYEVFRTRDTLAAVRYLVYEDSPVDFVIFDISDTLAIYPFKKEVTTVRRVDESAIT
                     SSLWETMKQKNINPMLALELSDVFAWTVDFFGLYPEDKFKVIYDELYVDSLPVGLGTI
                     HAAWFEHRGEKFYAFRYEQDSVASYWDEKGNSLRKSFLKAPLRFSRISSRFSGRRFHP
                     VLRIYRPHSGVDYAAPAGTPVMAIGDGVVIDKGYNESAGNYIKIRHNSVYTTGYNHLS
                     RFGKGIAEGVRVKQGDIIGYVGSTGYATGPHLDLRFWMNGKPVDPLKVKSPPVEPIRP
                     ENLEKFRQAITPVIYQLDSITPAGDSLRASR"
     gene            43997..45040
                     /gene="thiL"
                     /locus_tag="PK502_00480"
     CDS             43997..45040
                     /gene="thiL"
                     /locus_tag="PK502_00480"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPG99323.1"
                     /translation="MSNQERKTELYELGEFRLIDRLTGSFGKLHETTIMGVGDDAAVV
                     DVGSKYMVITTDLLLEGIHFDLTYFPLKHLGYKAVVVNLSDVYAMNATPTQITVSIGV
                     SKRFGVENLEELYAGVKMACERYGVDMVGGDTSSSMTGLTISITALGEVSKTDITYRR
                     GAKANDLICVTGNLGAAYMGLQLLEREKRVFESSPGAKPKLEGNSYILERMLKPEVRK
                     DTLQKLRDNKIKPTAMIDISDGLSSDLLHICKNSGVGCKIYADKIPIDSQTAQMAEEF
                     GIEPLVAALNGGEDYELLFTIPLEEFQNVSKIDGIATIGHITAESSGANLISPAGDEI
                     RLTAQGWNALAKE"
     gene            complement(45139..45522)
                     /gene="gcvH"
                     /locus_tag="PK502_00485"
     CDS             complement(45139..45522)
                     /gene="gcvH"
                     /locus_tag="PK502_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPG99324.1"
                     /translation="MNIPSNLKYTKDHEWLKVEGNGEATVGITDFAQSQLGDIVFIEV
                     ETVGETLGHEEVFGTVEAVKTVSDMFMPVSGEVLEVNPKITDQPDVVNKDPYGEGWIV
                     KIKMTNPAEVNNLLTAEQYKSVVSE"
     gene            complement(45722..53065)
                     /gene="sprA"
                     /locus_tag="PK502_00490"
     CDS             complement(45722..53065)
                     /gene="sprA"
                     /locus_tag="PK502_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HPG99325.1"
                     /translation="MFVFSCFLLFTPRVSIGGQVYEQTVNLQDTTRLPYPIKSENFLT
                     PATVRSPLFLNQPSNVTEVVEYDPTTKKYTVYQRVGGYNVGLPRVMSDEEYQNYRVEQ
                     SIREYWRQKQTGETVGKGDGILPRIQVGGESFDRIFGSNVIEIIPQGNAELVFGISSS
                     KTENPALPVDQRKNTTFDFQSKIQMNVAGKIGEKLKMEVNYNTEATFDFENNVKVEYN
                     GFEDEIIQRIEAGNVSLPLPGTLITGSQSLFGFKTEMKFGKLYVTSVVSKQNGQTQVV
                     EVKGGAQVRDFQIRADEYDANRHFFLSHYFRENYNRALANLPVINSGINITKIEVWVT
                     NKQANFENSRNLVAFADLGEADPNIFASSIFHQTVPGPADNSRNTLYELMTTTYSGIR
                     DIAEVNSTLLPLEGSGFSGGRDYEKIENARRLSSNEFTFNPVLGYISLNSPLYNDEML
                     AVAFEYTQDGKTYRVGELSTDGIAAPQALVVKLLKGTNLSPSLPTWKLMMKNIYSMNA
                     FQVSKNEFRLDVLYENDKTGTPLSYIGEDAIANIPLIRVLNLDNLNNQLDAIPDGVFD
                     FIEGVTINPAGGKIIFPVLEPFGRDLKAKFTNPAIADKYVFQELYDSTLTRARQMAEK
                     NKFILSGSYQSAGGSEIYLNAPNIPKGSVVVTAGGAKLQEDVQYIVDYNLGSVRILDQ
                     ALLESGTPIRISVESNALFSLQTKTLIGSHFDYRFSDNFNVGATIMNLTERPLTQKVS
                     FGNEAISNTIWGLNTSYQTDSRLLTRMVDFLPFIQTKEKSTVTFDAEFAHLIPGHSRA
                     IGKAGTVYLDDFEGSSTKLDQHYWTEWKLASTPQGQPALFPEASYSNDLRNGMNRAML
                     AWYFIDPLFLRNTSLTPSYIRNNKELQGSHFVREIFERELFPNRNTPQGQPTNISVLN
                     LSFYPDERGPYNYDDINVDPDGRLLNPQNRWGGVMRSIGTTDFETANIEYIEFWLMDP
                     FVYDSTMSGGDFYINLGNVSEDILRDGRKSFENGMPTTQAMEKVDTTVWGRVPRKQYL
                     PVGFDNDPANRKYQDIGLDGLSGEDLDGDGIPDEQSFFQNYLTQLRFRVSNEEVMAKS
                     DADPSNDNFIHFLNNQYDQSKATILDRYKYFNNTEGNSPVATGTTSQQAYSTPDVENL
                     SEGDITMSESEAYYQYRISLRPADMEVGRNYITNVISGEKDKLRNVPVKWYQFKIPIT
                     EYERVVGPIDDFKSIRFMRMFLRGFQDTMILRFATLELVKSEWRKYNYSLIQGQEGIP
                     SGDLPTTGFAISSVNIEENDKRTPVNYVLPPGVDRVIDPSQPQLTELNEQAMEYRVID
                     LADGDARAAFKNVNIDIRQYKKLKMDVHAEMVEGYPLSNGDVSAFIRIGTDYTDNYYE
                     YEIPLSLTPYGYYFNSRESDREMVWPRENLINIDLEKLVEVKVARNDAMRTPGSMVTQ
                     RTLYTVDDGDAQMKVVGNPNLSNVKVIMIGIRNPANGKGPTDDGMPKSVVAWFNEFRL
                     TNFNDQGGWAANARVSAKLADLGMINVAGSTVTPGFGSIEKKVNERSKETIYQYDINS
                     TLELGKFFPEKLNVRIPMFLGFSESISNPQYNPLDPDVTLSSSLNSYNPSERDSVKRI
                     VQDYSRHRSINFTNVKIDKTTEKPHFWNISNFSVSYSYSELFSRNIRTDHKIQRDIRG
                     ALTYNFNKQPKYIVPFKNVKWLNSKYFRLIKDFNFNLTPAQLSFRTDLDRSYFEQQFR
                     NVSNPSAVLLPTYSKDFLWNRNYALNWDITQSLKFDFTASNVARIDEPEGMVDRNRDP
                     KGYEHWRDSVWQNIKNFGRNTMYNHQFNLSYSLPLSKIPFLDWTNASARYTGIYRWQA
                     GPIMADTSRFDPGNTVQNSNTIQLNGQININTLFNKVGYLKRINQEFDSRGRGAAPKT
                     KTVKYEEGGVNIRAKYAKNINHKLKTDKVTVRVYDEKGNEVKVKSEVKSDTRVAIRAD
                     ADYSNCRVVVEGKVPDRPNPLKFAWEGTLRLMMSVKSLSLSYSETNGTLLPGYKPGTR
                     YLGIDSYNGFTAPGWEFIAGWQDPEIAWKAATNGWLTHDTTFNTPVVFTHNENLNFRS
                     SIEPVPGFRIELTANRTYSRNHNETYRANALGSYSSLSPLTTGNFSISVLTLGTTFEG
                     KNRDAKSRAFERMKSNRLVIAERLRDQRVANPGQGYDPMAPPDSLGFPVGYGPLSQDV
                     LRASFLAAYTNTSPQKVTLRDFPVIPMPNWQITYDGLAKIRPFKNLFKTFTLSHGYRS
                     AYTIGAFTTNLNYLPEDDGFSYVRNINGDFVPKREITNVSINEQFSPLLGLDMGWQNN
                     LTTRLEFKTSRSLAMSFANNQLTEIYGNEYIIGAGYRFENLPLVFQSEDGGKSAVKSD
                     LRITVDLSIRENTTILRKLVEDYSEIIAGQLVNTIKTSADYRINEQVTVRLFFDRTLN
                     APKVSRTYRTTNTSFGFSVRFELVK"
     gene            complement(53123..53707)
                     /gene="ruvA"
                     /locus_tag="PK502_00495"
     CDS             complement(53123..53707)
                     /gene="ruvA"
                     /locus_tag="PK502_00495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPG99326.1"
                     /translation="MYDYIVGKLAELTPTHAVVEVGGLGYHVNISLQTYSAINGMNEV
                     KLYLHQVIREDAHLLYGFFEAKEREIFRLLISVSGVGASTARLILSSLNADELQAAIL
                     TENINLIKQVKGIGLKTAQRIVVDLKDKVVGDDFASKQIMPAAHNTSRDEALSALVML
                     GFSKSATEKVLDQIIREGGELTVEGLIKSALKRL"
     gene            complement(53834..54886)
                     /gene="pdxA"
                     /locus_tag="PK502_00500"
     CDS             complement(53834..54886)
                     /gene="pdxA"
                     /locus_tag="PK502_00500"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPG99327.1"
                     /translation="MSDEKIRVGITQGDINGIGYEVIIKTFMDPRMVELCTPVVYGSP
                     KVAAYHRKALNVENFSFNHIRDAAEANPKRVNIINCMDDEVRVELGKSTEEGGKGSLL
                     ALEAVMPDLKAGKIDVLITAPINKANIQSAKFSFPGHTEYLASSFNAKEFLMLMVGDL
                     MRVGVVTGHVPLSKVSSLITRDTVLGKIRLLNQTLVRDFAIRRPRIAVLGLNPHAGDA
                     GLLGGEEESAIIPAIKQAADEGIAALGPYPADGLFGSDGFTRFDAILAMYHDQGLTPF
                     KAICFDSGVNYTAGLPVIRTSPDHGTAYDLAGQNLANPNSFRNALYLAIDIFNNRKLH
                     DQLTTNPLSVSSSENG"
BASE COUNT        15340 a        13073 c        12223 g        14594 t
ORIGIN      
        1 cttttaccag cagtcagcca accacataca ttatggattg tgaagaaggt atgcagcccg
       61 attaatgtca tcaaccgagc ggatgggatt tggaggggtg aggtagcgtg ccaaaaactt
      121 gtagatttta acgcgaattt cacgtgcagc cttggtgtca atcctgtcga aagagtgacc
      181 accgggcata tctttgaaaa tttcatactc aaacttctta ccttcggctt taagcgcttg
      241 aatgaggtgc tccacctcaa tcatataaac atcatcgtcg ttggtattgg tgtgtatcaa
      301 aagtggtgta ttcttgaatc ggtcaacctg ccaaacaggc gagcgacgct tgtactcggc
      361 aatgtcctcg cgggggctct tgtcgatgtg gtagggagcg gagtataact ctcggtagtc
      421 atcggtggag taaccaaacc gcatgatgag gtctgaaacc ggaaccccgg caaatgcgca
      481 ggcataatcg ttggggtggt caaaaatgtt gtgcagcgta atcatgccac catggctcca
      541 gccaatgatg ccaacccttt tctcatcaac aaaatcgtag ttttcaacca tatagttacg
      601 gctggcatac acgtcctcgt tctccaggcc gccatagtcg atattctccc aaaaactgcg
      661 tccatacccg gtgcttcccc tgtactctgc tgccactaca atgtaaccct gagccatcat
      721 ctcgcggatt atgtgggtat ggtaggtagt aaagtcagca tgaacacctc catgagggaa
      781 taccagcaag ggatactttt tagaagggtc gatatattta ggaataaaaa cgtaggccca
      841 gaacttaacc ggattggttg cccccatggc agtaggattt ttaacgtttg caggaggagg
      901 cccataaatg taaaccttgt caacaaaggc tacatcacct accctattaa accaaagaac
      961 atcatccatt tgcttctgca tctcatcgag cctatggttt aggttgacat tctcctcctg
     1021 aagggcttcg agcttacggt tgatttcatc ggcctgctgc gagtatgccg ataaaaacaa
     1081 gcaaaacagc ggtagaacaa taaatttttt catctggttg taggttttgt ttgatataaa
     1141 gttaaaacaa aagtttgatg aatgctacca ccaaaagctt aaccgccagt cttaaccggc
     1201 tataaggaaa tgcaattttc taatcgggta ccaccgattt taccacaggt acccttctac
     1261 tgaaaagcaa ggcggaaata gcacaaagtc cccctgcaac agccatggtt ggctgaattc
     1321 caaccgaagg gctaatggcg cctagcaaca ggctccccaa aggtggaaat ccgttgaacg
     1381 aaagactgta gagcgacagg atacgacccc gcttatcgtc gtcaacaata tactgcagga
     1441 gcgtgttggt tgcagcaagc tgcacaatca tcccaaaacc agtaaagaag agtaggccaa
     1501 ccgaaagcgt agcataggat gacaatgaaa aagctgccag cgatgttcca aatgttagcg
     1561 agctgtagag gataatattt ggaatgacct ttatgctctt aagcgacgcc agcaggatgg
     1621 ccccggtaag cgcaccggca cctaccgccc cggtaagcag tccaagcatt tgcgagttac
     1681 cgtgcaggat atcacgtgca aataccggca taaaagtttg gaagggaaat cctagaaacc
     1741 ctaccacaac caggagagag agcacatacc tgataggcag gaaattatag gagtaggaca
     1801 atccctcctt caactccttg aatatggatg aattatgtgg ttttctttca acttgggcca
     1861 cccggatatg aagtagggag gcaattacgg ccataaagct gattccattg accagaaagc
     1921 aaaaaccctc tccaatccat gcaaccagaa cacccccaat ggcaggacct atgaaacgtg
     1981 ccgagttaac caacgttgag tttaaagcta tggcgttttg aagcaaatcc ttatcgccta
     2041 ccatttcaag cataaacgaa tgcctgaacg gagcatcgaa agctattgcg attccgttca
     2101 aaatggaggt tacaaaaatt tgccaaacct gtatggtatg tgtaaagtag agtgtagcca
     2161 gggttaatgc aacaatcatg gaaataccct gagtaataac aagtactttg cgcctgttca
     2221 acctatcagc gtaaacgccg gcaaagggca tgagaattaa cgagggtatt tgaccggcaa
     2281 aactaacagc acccagcaaa aaaggagaat ttgtcagcct ataaaccaac catcccaacg
     2341 ctaagccctg gatgaaggtt cccatgagag aaactgcctg gccgtaaaag aaaatcctaa
     2401 agttgggact ttttaacgct cgaaaagcaa acttgatttt gcttaggtag gatgcaggat
     2461 taagatgaaa agtcattatt atttagatgg gtaggtattt ggttatgcaa aaatacaaaa
     2521 tatatgctga cgtcgtgtat tgcccatcac gcttgattga cctcccaagg gtaggaaact
     2581 taatgggtaa ggcatgttag tcattaaagt taaaaaatgt tactccccta atcttttgtt
     2641 aattctgatt tagctgtagc gcaaccatcc gtaatgtgat aatagctaca tttgcagcaa
     2701 gtgaaaaaaa acgtatttct gtgaagaaag taaaaacaat tgccctggtt gcgcatgatc
     2761 accgtaagcg cgacctggtt gaatgggtcg aatggaactg gcaggaacta cttaagcaca
     2821 agctcatctg cacgggaaca accggtcgtc ttattgagga atcgttacag aaaaagaaga
     2881 gtgacgtagc agaccacatc cagctaaccc gccttaaatc aggcccgctg ggtggggatc
     2941 agcagctggg tgctctaata gccgagggca aaattgacat tctgatattc ttctgggacc
     3001 ccatggaagc ccagccccac gatgtggatg ttaaagccct gctccggatt gcaaccgttt
     3061 acaatattcc cacagcaacc aaccgctcaa cagcagattt tcttatcagc tcccccttga
     3121 ttgatgagga ttacaaccct attctgtttg actacagcca ctacatgaat cgaaaggtat
     3181 tttaacaagt cctttctact tcaattttga ggttaagcag cgcctgaatc tggctcgaaa
     3241 tggatttctt gacagtttca gggcatacca attacaaccg gcttaatgca ggagttgcac
     3301 tagcactgat aacttctact gtggtcttgc tatgggccta tgtgctctcc acattaaggc
     3361 ctaatatctt tgatcatcgg gcaacaaggt gttcaatttc agcgccttat cgcaccactc
     3421 atcagagcat aacaaatcat aaaagctgac tagacaacag ccactcatag aacattatca
     3481 ggcataaata caaaatctat gagaatgtgt ttactaaaat tttgaattgt gatttaaata
     3541 ttgctaaatt tcaccaaaat ttcataaaca aaaacgctgg ttatgaacca caataaaggt
     3601 aaatggtgca gaataggcag catggttttg cttacagcac tacttacagg atgctacgat
     3661 ttttcaaatt ttgaaaattt caaggttgaa cctgtaaacc cctccatagt tgctccggtg
     3721 gttaactcca ctataacttt taaggagctg gccgaaactg aagacgccaa taccattgtg
     3781 attgttaaac caggcgatac caggttttac ctctccttcc gcgataccat ggaaattgcc
     3841 aataccagtg gtgagttttc aattccacca tttgcctact ccaaaaccta tcaacccgat
     3901 gcaggtgaaa ttcctccggt tgcacctcca gctggcgaaa gtattacctt cagccccaaa
     3961 accttcaccc aaacctggca acctgacgcc gattctgagc taaaactcat caagctatct
     4021 cagggaagta tgaacgttac cgtaaccaac aacttcggaa acatcattag cggcagcatc
     4081 acgttaaact ttctactaac ccctcagaat caaccctaca ctatcaacat tcccaatatc
     4141 aatcccgctt caagtcaagc catttcgcta acaaacctgg cgggctacag ctttaacctt
     4201 ttcgacggca ccagctacaa cacccttaca tttaccgcta ccatcaccct cacatctatg
     4261 ggcaattcta taaaccctgg cgataacatc aatatcgaca tttcggcaaa caatctcgac
     4321 ttcaccgaaa tttacggcaa ggtcaataaa accatcaacc tttccgacca atcttttccg
     4381 ttggatatat tccgttccac ctacattgcc gaccagcacc tggccgagcc cagccttatg
     4441 ttaaagttcg ccaacagcta cgggattccc ctgtcgctta acgttaacca gctggtagcc
     4501 agcaacgacc aaactggatc catgaacgtg gttaacgaaa ctgttcctcc cgatgggtca
     4561 ctactcattg gtgcacccaa ttcgttaaac tatgttggga gcccatccca ggaatttgca
     4621 atggactccc taaagctgga ctatcaaaac tccaatattg aggatgtttt cgactttgcc
     4681 cctaacaatt ttacccttaa atcgggaatt acccttggcg atgcctcaac cagccacgac
     4741 tactttgtta gaaaaaactc caaccttacc cttttcagta atgtagaagt tccgctggtt
     4801 ggctgggttg agactaacgg cattcgcgat accgtagaga tagaccttcc ggatattgag
     4861 aacgatttga acatggaaga tgacgacgag ttgagcctaa ctctgaagtt caggtttaac
     4921 aacggtattc ccctgaatgt ttacttccag gcttacttta ttgacgatta cgaccatgaa
     4981 cttaccaggc tatacacaaa tgtggcagaa gagcagctta ttgaaagcgc tgcagtaaac
     5041 ccatcaaccg gaaaagtcac aactccaaac gtaaaaattt ctgaaatttc gattgacaga
     5101 aataggtata aacaaatgca gggtgccacc aagctcgttc ttcaggtacg gtttaaaacg
     5161 ggggggagca ctcatcaaaa catagttgtt gaaagcacca actccataga tattcagatg
     5221 agcatcaagg caaaaggaac ggttagattc gattcctagt gttaggagta tgccgataaa
     5281 tagctataca gctacaaatg aagcgcccac gatttgattt aacgcaaccg tgaataagtt
     5341 aaacttgaat atttgccttt ggcaagcctt atgaactcca aatgctggtg aaaactaaaa
     5401 agtgaaagca caacttattg tcaactaata atattaacat acttttcgga tgaaacgtct
     5461 gtctttacta gcactaatac tcaccgtcgg catttatgcc aaggcccaat cggagctaac
     5521 gctaccattc atgcccggtg tttttcagtc atcgttcgta aatcctaccg ttatccctga
     5581 acatacattc agcatgggct ttagtattta tacccaggta gttacaaatg ggttcgtgcc
     5641 taacaacgtg cttatattca gggatgacac catgcacttt gaccccaacc tgctgctcga
     5701 cgatctcagg gataaaaacc tattctacat gggtcagaat acggacatct tccatattcg
     5761 tacaaaaatt cttaatgggt actactggtt tgccattagg caaaaaatgg aatcgtcgtt
     5821 tttctaccca tacgatcttt tccgcatggt tattgagggt aatgaaccct acatcggcca
     5881 aaccctcgac ttctcaaacc tgaagatttt cacctcccta ttcaacgagt actcctttgg
     5941 catgacgaag gagtaccccc actggatttt tggtggcagg cttagcctgc tacaaggcct
     6001 aagcaacatt aattttaacc ccacaaggct tagcattaag gtggaagatg aaatgtacgg
     6061 ccagctggcc aacgcagatg cccagctgag aacatcgggc attcccaaag atggcaatgg
     6121 cgatcccagc ttcgatcatg ttgaagaacc tgagtggata accgattact ttaccaactt
     6181 caagaacaaa ggatttgcgc ttagcggggg tgctacctac aggtatgacg accggacacg
     6241 aatttcgctt tcattccgcg acgttgggtt tatcacatgg aaggacagca ttgaaaccta
     6301 cacccttaac ggaaacacca acttcaccgg gctggatatc atgagcgacc tcctttacgg
     6361 caacgatgtt gaggtggact ccatttttga aaatatcctc gatgaggatt ttcaccgtga
     6421 caccatatac aacaagtaca ccacatggct caacccgaag tttaacctta cggctagcta
     6481 cgacctggct cgtagaacaa ctgttggatt ttccttttcc ggagtagtta accagaaact
     6541 ctacaccgca ttaaccctaa gcctttcgca ggggattggc cggtttttca gcattgttgg
     6601 cacggcatcc atcaaccaga gatcttttac caatctggga cttggcctaa tgctgaagcc
     6661 gggcccactg caaatttttg tggttgccga caacctttac gccgcccata acccgctgac
     6721 tactacaaac ttcaacatta gggtaggtgt gaatcttgta ttcggtagaa taaaccaacc
     6781 cgaaggatta cccttccgtt aatgcaacac aaattgatga atttccgact taatccgttt
     6841 cacacatgag agtatcagga attaagaata aaatagtagc actgcttctt ggaagcatgg
     6901 tagcgttagg cctgcaggcc cagacctctt tcacccctat caacctggga gatagggtaa
     6961 attctgaata ccctgaaatt aaccctatac tgcatcccga tggtaaaact ctgtattttt
     7021 cgagggccaa caacccagag aatacatggg gaggcgttaa cagccaggat atatggtact
     7081 ccgttttagg agacgacgga acatggagcc aggcccaaag gctaccccgt gctgttaata
     7141 ttggtaggta caacgccctg tttgccatcc tgaacaacgg gaaaactttt ctcattaatg
     7201 gccattactc tccaaatgga aagtactgga ttgaaagggg actttcgact gttgagcaag
     7261 ttgagaatga aaattggggg aaacctcaaa gtattgaggt aaagggttat agcaacaaga
     7321 ataggggaaa aaccacaact atattcgtaa gccccgacga gcaaaaaatc ttcatggcat
     7381 tcagtaaaaa agtaaatggt aaaaaggcca gcatttacct gtccgagcga aaaggcgaca
     7441 ggcgttactc caaacctaaa aaaataaatt ttctaggaaa taacaagtgc gaaagcatgg
     7501 aggcaccttt ccttacctcc gatggtaacg ccctgtacta cagctgcaag attaatggtg
     7561 ataacaacct gtacatgagc caccgcaccg atgataccta ccgcagctgg tcggagccca
     7621 taccgctaaa cgatacaatt aacactccgg gttgggaatg ctactaccac ctgaacagta
     7681 agggtagctg ggcatacttc tgctccaata caaactccct cgggaagtca gatatcttca
     7741 gggttaaaat atttgaggaa aacccgttcg taaggcttca cgggttggtt ctcaacaagg
     7801 ctgatcaaaa actaatgctg gccgatacaa gctacatctt aaaagtgaat ggctctgaac
     7861 cagaaaagct aaaggttgat agggcagcgg catcctacga acttctgctc ccatttggcc
     7921 aaagctacca gcttcaaccc gaaatgaaaa actggattgg aatacccgaa ttatacgatg
     7981 ccacgggcat taaggagttc actgatgttg agctaaacct ttactttgag ccaattccct
     8041 acgttaaaat atttggtaag gtaatcaata ccagaaccaa cctacccatt tctcccgaaa
     8101 taggcttcaa ggtgcttata aacgggctag aaaatgattc ggtgaagtac attagggata
     8161 tttcaggcta ccaggcacta ctgccactag gccagaagta tgtggtttcg cttaacctcc
     8221 caaactacag gtcggaagcg gatacggttg atgtaagcaa tatatccttc tacaccgaga
     8281 aacaggtaga ttttcatgta acctcagcac catgggtcga agtttacggt attgccatgg
     8341 ataacgtttc ctttaccccc attcccggct cctcttcacc caagctactg gttaacggtt
     8401 accctgccga tagtatcgct attgatccgt ttaccggaga gtttaccatt aaactaccct
     8461 acgggataaa acacaacctg gcaatcagct caaaggatta caacccgttg gataacgtgg
     8521 ttgatttaac cggctacgtt gagtttaccc gtataaatca cgacgtatat gctgaacgta
     8581 aggatgccaa tatggcaacg ctttacggta aggttatcaa tctaaaaacc ggacaaccag
     8641 ttgaatcgag tattcctgta aaactcaagg tgaatggagt tgaaaccagg gcctttattt
     8701 acgattctac cactgctgcc tacaccttga aacttcctgt aggcgtttca tacgacattc
     8761 ttccatcggt aaaaaacttt tacaacaagt acgagcaggt tgacctgacc aagactaaaa
     8821 aaggcaccaa ggttcccaag aacttctaca ttacacctat tgaggttgga caggcggtta
     8881 acattgactt tatctacttc gaaacgggta aatcaaccct gaaaccccag tcgttccgat
     8941 cgcttaatgc cctggtggat ttcctcaacg agtaccccaa tgtaatagtt gaaattggcg
     9001 ggcacacgga caacacgggt tcggcagcta taaacgaaaa aatatccaac cttagggcaa
     9061 aggctgtagc tgactatgtg gtatcgatgg gcatccccgc ctcgcggata acttcaaaag
     9121 gttatagctt caacaaacct aaagccagca acgcaacagc aaccggcagg gctcagaaca
     9181 gaagggttga gtttaccatt gtaggaatat aatacaatct gatgaaacga acgagtgtgg
     9241 tcatgtgtaa gaacatactc gttcttttta ttgtttaaag caatacccgg taccaatcaa
     9301 taatgttggc tgtgtcaacc ttttactgcc tattcatctc ccgcaatgaa accaaaaggc
     9361 atttttacgc caacctgttg actaccggta gtagtttttc acacagaagt tgaaacatgt
     9421 ttgccctaaa gctaaaattg ctgctaagct gttttgtagc cgtgctggcg ctggttaacc
     9481 ccatccaaaa ggtattcgta atgacaagcc ttcaggatca gttcagcgaa gccgatacca
     9541 aaatggttgc tcttaagtcg agcctaacag cattgctcat tctgttgttc ttcctcctgc
     9601 taggagaggt tgttttcaac tacgttttcc atgtcgagct gtacgccttc cagataacct
     9661 gcggcgtggt tttgttttac aatggtatta ccggattgca aaagggcatt tttataaagg
     9721 ttgaccagaa catcagcata cacgatataa cggccgttcc cattgccata cccatgattg
     9781 caggaccagc aaccattaca gctgctgtta cctttccagc ccactatgga cacttcaaca
     9841 ccattgtttc cattgttgct gcccttgcca ttaacctcct cttcatgctc tacgccaagc
     9901 atatagcaag attcttaaac cgtttcaacc ttatgaatcc actggttaga atttttggac
     9961 ttatagtggc cactattggg gttcaaatga cgctaaatgg aatttggggg tttgtggttt
    10021 cagttgctgg acacgtgtaa cttcgagcta ataatcacca caaaaaatcc tcatacattg
    10081 ctatatttgc agaacataag aaacattatc ggtggaacaa aaaaatttta tagacattga
    10141 tcaggttatt gcctcaaaaa gccctacgct tcaaaaaagc ctcccccgtt tcattgtcaa
    10201 ctacctaaag cgtatagttc atcaggacga aataaatgag attttggcca agcatgggca
    10261 taagcaggga gctgagtttg tggaggcagt tctcagcaca atgggggtaa actactcggt
    10321 tgaggggttg gaaaacctgc aggataacca acgctacctt tttgcatcga accacccgct
    10381 cggtgggctc gatggcatgg tgcttattca cctgctggca aaaaggtttg acgaggtgaa
    10441 gtttcccgta aacgacctgc tcatgcacat taaacccctc tccagcgtat tcataccagt
    10501 taataagctt ggaacgcaga gcgcttcaaa cgccattcag cttgaggagg cttacagctc
    10561 atcggcacaa attctatact ttcctgccgg cctttgctca cgcaagcaaa agggtagaat
    10621 tgaggacctg gaatggaaaa aaaattttat tgttaaagcc attaaacatg agcgggacgt
    10681 ggtgccggta ttttttgacg ggcgtaactc cacctttttt tacaacctag ccaggttacg
    10741 ggctaagctg aggattaaaa ccaacatcga gatgctctac ctttccgatg aaatgttcag
    10801 gcaaaaagga aagagcatta cggttaaaat aggtaagcct atcccattca gcacctttga
    10861 caacaccagg aatgcccatg aatgggctca atgggttaag catgtggttt accagatgaa
    10921 gcccgcttca taaactgtca aattgatgca gtaaaaacca acccagtaaa ctccaattct
    10981 agaagatact agccatgcag gaaataatcg aaccggttga ccggaaccta ctaatggcag
    11041 aactcacccc cgagaagttc gttaggaaca ccaacaatgc ggaaaatgag ctctacctct
    11101 ttaccgccca tgattcgccc aacctgatgc gcgaggtggg caggctaagg gaatttactt
    11161 ttcgctctgc cggtgggggc accggagagg aggttgatat agatgagtac gacatctgcg
    11221 ataactgcta caagcagctt attgtttggg atccaaagga aagggaaata cttggaggat
    11281 acaggttcca cagcgttgac ccctcaaagg gcgatgaaat taacctctcc accaagcacc
    11341 tgtttaattt ctctgaaaaa tttgtaagag agtatatccc taaaatgatt gagctgggac
    11401 gctcttttgt tcagcctaag taccagtcgc tcagcagggg tggcaaagga ctttacgccc
    11461 ttgacaacct gtgggatggg ctgggcgccc ttatcgtgct taacccccac atcaggtact
    11521 tcttcggcaa ggttaccatg taccgctcct acaacgtaga ggctagagac acactcctct
    11581 acttctttga aaaatacttt aaggacaacg aacagctcgt tacaaccata catccaattg
    11641 aggtgaacat ggatcgggat aaaatggaaa ggctattcac cggaaaaaac tacacggagg
    11701 attacaaaat tctatcgcac aagataagag agcttggcga gaaaattccc cccctcatta
    11761 acgcctacat gaaccttagc ccatccatga aagtttttgg cacggttata aacccctact
    11821 tcggaaatgt tgaagaaaca gcaattctta taactattcc ggatatttac cagcataaaa
    11881 tggaaaggcg caccggcaca ttcgtgagga agctggccgg taaataccta agaccctacc
    11941 gaaaggaaag ctgaccgcag gggagcagaa aaccctgctc agccgggttc aaccagcaaa
    12001 agatttacca tgacagcacg gacagagaaa gttcactgtt actggtatgc cctgttgtta
    12061 cggccctcaa taaagttgat aaacgagttg ttcaccaccc tatttccacc cggagtaggg
    12121 tagttgccgg tgaagtacca atcgcccaaa tcgttggggc aggccaggtg aaggttttcg
    12181 attgactggt acacaatttt aaccttagcc ctcatatcct ttggggttaa tagctgggcg
    12241 attttttcgg caagttgatc gtccgtaaac gggctgtaaa tctccttgac gtaattcaca
    12301 atttcctctt taggcagata ttgctgttcc ttacacttcc ggtaaacctt gttgatgatg
    12361 gcctcctgac ctgtatcgtg cagcaatcca acagcggcct tgaaagctac aaaatcgcta
    12421 agcttggcca tatcgatgcc gtagcaatcg ggatagcgaa tttgaggtga ggaggacacc
    12481 accacaacgc ttacgggatt tagcctgtcg agtattctta gaatgctctc ctttagggtg
    12541 gttcccctta ctattgaatc gtcaatcacc accaggctgt ctatccccgg cctgatggcg
    12601 ccataggtaa tgtcgtacac atgcccaaca agatcctccc tccccttatc ctgggaaata
    12661 aatgtacgaa gcttaacatc cttaacggca attttttcga ctcgtaaccg aatggcgatt
    12721 acctcatcca aatcgctctc ggtcaatgag ctgctctttt ccaaaatcat ccgcttcttc
    12781 tccaccttca ggtaatcctc caccccctta accataccgt agaatgccgt ttcagcggtg
    12841 ttgggaatat aagaaaacac agtattggaa tggtcataat taatggcctt caggattgcc
    12901 ggggttagca gttcgcccaa ctttttacgc tcctggtata tatccctgtc tgagccccgt
    12961 gaaaagtaaa tcctctcgaa tgagcatgac cttcgttgct ggggtaccct tatctcctca
    13021 tgggtgatag ttccgttacg cttgataatc aagccatagc ctgggttaac ctccttaaca
    13081 tcatcggttg caacattcat aaccgtttgt atggccgaac gttcagaagc cacaacggca
    13141 aattcgtcgt ttaaatagta gaacgctgga cgaatgcccc atgggtcgcg aattacaaag
    13201 gcatcgccat gaccaataag accggcaatg gcataccccc cgtcaaaatc ctttgttgct
    13261 gcctgaagta tcttgccaat gttaatgttc tcggcaatta gcttgctaat ctccatgttg
    13321 gagtacccct cattcttata ctgtcgaaaa acctgctggt tttcctcatc cagaaagtgg
    13381 ccaatctttt ccagaacagt tacggtgtcg gagtaatcac gtgggtgctg ccccaagctt
    13441 accagcttgt taaagagctc atccacgttg gtcatgttaa agttcccggc cagaaccaga
    13501 gtacgtgatt tccagttgtt gtcgcgcatt acgggatgaa cgtaatctat ggagttgttt
    13561 ccaaaagtac cataccttaa gtggcccaag tatatttcac cggcaaaggg aatattttcc
    13621 ttagcccagg ccgaatcacc ggctagttca ggtctatgcc cgtactggtt aatgctccta
    13681 ttaacggcat caaaaatatc cttaatggaa gaatccgaat tagaacgctc cctgtcgatg
    13741 taccgcttcc ccggttccaa atcgaactta agagaggtta gccctgctcc atcctgtcca
    13801 cggttgtgct gcttttccat tagaaggtac agcttctgaa gcccatactg ccatgtgccg
    13861 tacttaaggt ggtagtactc cataggttta agcagcctaa taagcgcaat accacattcg
    13921 tgtttaatct gctcgctcat tttatgtttc ctgtatgata aaaaaaggta gattaatcta
    13981 cccgtttaca atattccttt tttattacga gaaagggttt ccaaaacctt actggctata
    14041 gactgggagt tgagcccata cttctgcaac agctcggctg gagtaccaga ctctccaaag
    14101 caatcgggca ttccaacaat ttccattggc gttgggcaat atttcccaag aacttcggca
    14161 acagctccac caagtccacc gtttacctgg tgcacctcaa cagtaaccac aaaacccgta
    14221 tggtgggcta tatctacaag tagtggtata tctaaaggct taattgtgtg aatattaacc
    14281 accatagcat taaccccttg cccggccaga atatgggcag cctcgattga ctcccaaacc
    14341 tcatgcccgg tagctacaat tgcaacgtcg tcgccattca taagaacctg ccccttcccc
    14401 accactagct tctgatcctc atcggtaaaa ttggggacgg gctcacgacc gaagcgaatg
    14461 taaacaggcc cattcacctg ctgcatagca gcaatggtag cagccctggc ctgattggca
    14521 tcgcacggag aaataagggt catatttggt aatgccctga ggtaggctat atcctccagc
    14581 gcctgatggg tagcaccatc ggggccaacc gatattccgg catgagcacc acctataata
    14641 accttaacgt tgttgtagca tattgaaatt cgtagctggt cggcagcgcg tagtgcggca
    14701 aaaacgccat aggaggaaaa tacaggaagt tttccggcca acgccaaacc tgctgccact
    14761 gcggtaatgt tctgttcggc aataccaaag gaaaaaaaac gttcagggaa cctacgcttg
    14821 aaaccatcca acccaaccga ggcggttatg tcggcaccca gcaccaccac gttggggttg
    14881 agctccccaa tatcaaccac tccctcacca aagcccgttc gggtagcctt atcgccttta
    14941 ttcctgaact gttccatttt gtagagataa gtagtgagcg tgtagctcat caagaaactt
    15001 ttccgcctcg gtagggcttg gtgccttccc atgccagcgg taatcgtcct gaatgctttt
    15061 aacgccatat cccataagcg ttctggcaat aattaccgta ggtttcacat caccatgctc
    15121 tgcaacttca aaggtttcaa ccagctgggc aatatcattt ccatcacact ccagcaccct
    15181 ccagccaaaa gattcccatt ttgccctaag gggctcaagc cccataacct ccgatgttcg
    15241 gccatcaatc tggcagtcgt ttctatcaac cagggcaatc aggttattga gtttatgatg
    15301 ggcagcgctc atggctgcct cccagattga tccctcctgt agttccccat cgccatggat
    15361 ggaaaagacc cgccatgggg ccctatcaat tttagcggcc agagctaagc ctactgctac
    15421 cgaaagtccc tgaccaagcg aacctgcaga aagttcaagt ccgggcaatc catgatccct
    15481 tccgggatgt ccttgtaggc gcgagcccag ctttcgtaag gtgagaagtt cctccctggg
    15541 aaagtatccc gagttggcca aagcggcgta gagcaccggg gcagcatgtc ctatagatag
    15601 tatcagcctg tcccgctcgc tccaaccggg ctgaagcgga cggtgatgca gaacttgcca
    15661 gtagagcact gtaaaaatat cggcaagccc taacgaggct ccgagatggc ctgaaccagc
    15721 tgaggctaaa ctccgaacga catcctcccg aagttgtttc gataccttta gaagctggtg
    15781 caagtttgcc tggaccataa cactactcca gtggtggaaa gttaatccat tgcggaatta
    15841 taaatgcttt agggtaatct acaagaagct ggtggtaaag tttttgagcg ctctcccgag
    15901 tacggaaatc acccaccgaa attttccagt agggattgtc gtagctttgg taaactctaa
    15961 tatcagggta cttttccata aaagcattca tcactgcctc ggagttgcga cgagcgtttt
    16021 gccccagctc caggaatatg cgaatacgaa aaccgttcat ccccggattc ttagcattct
    16081 gaagtatgtg cagctccaac aggtttgaaa ccactggatc ctgggtaatt ttaacctttc
    16141 cctgctcatt gccttcgtgc tgattttgca caattccact taccccctgt ccgtgaacat
    16201 gctcagcggc aaatacacaa atcagaaaaa gcgagttaaa gagaagtgct ggtaaactac
    16261 gcatcggaat ataattgcac cacaaaagta gcaaaagtta gcctaattgc caaccgtcga
    16321 attgcggacg gaaattgtgt cggtaataat aaacccgttt tggtacttgt tgatgagatt
    16381 gttcagaggc tgccggggga ttttaatttt ttttacaatg gggaagctcg accccttaac
    16441 aaaaccctca acggttatca ttgtcagaat cttttcgccg gaaatcatca actttagggc
    16501 atcagcagaa gaatttaatt ttacttcaac agggatagag tagatttggc ttgagttggg
    16561 tggaatttca attctatttt taactctaac agttcccagt tccctttgtt ttatccacga
    16621 tttaaactca acctctttta cagttatcct tttggaattt ggattctcaa tccctactgc
    16681 taaattcagc ttaacaacgt cattctttaa cccgttatac tcaactttgt ttatcccgtt
    16741 gattttaatg tcctgaaatt tgggtgcgca tccggacatt aataccatga agataggcac
    16801 aaaagcagca agaaaccttt taaccttcat ctattgtaga attttaattt aattagcaat
    16861 aactgttcca tgctgcttat aaaaagaaga aaaagccatc gaacaagcta gccgatggcc
    16921 tcaatattgt aaaacgttta taaattctac tactcatttt ccatagcagc cacaacctca
    16981 tcggtcattt ccaggttatg gtaaacattt tgaacatcgt cgttatcctc aagctcgtct
    17041 atcagcttaa ggaatctaag cgatgaatcc ttgtcaagtg ttttatagtc attggggata
    17101 cgctgtaggc ttgcattttc aacttcaaca ttcagctcct ccagcttttt gttcatccgg
    17161 ccaaagtctt ccatttcggt agttactacg tagaaatctc cattgtcatc gatactttgg
    17221 gcgccggcat ctatcaggtc aagctccacc tcgtcgagct tcagcttatc cttggcaata
    17281 gtaaaaatgc ccttgcggtc gaaaagaaac gataatgagc cgttagttcc aaggcttcca
    17341 ccacgcttgg tgagaatgga acgaatagcg ctaaccgttc gattgttatt atcggtaaga
    17401 cattctataa ataatgctat tccacccgga ccataaccct caaaggtaag ctcatgaaag
    17461 ttttcgggat ccttctcagc cttggatatt gcccgcataa tgttatcctt gggcatgttc
    17521 accccctttg cgttgtttat agcaaggcgc agccgggggt taccatcagg atcggagcca
    17581 ctttccttga ccgccacggt aatttccttg attattcggg aaaactcctt ggagcgtttg
    17641 gcatcaagcg ctcccttttt tcttttaatg gttgaccatt tattatgtcc tgacataaag
    17701 tatttgttta gcttattcaa ttataccatg caaaagtaac cgcttgtttt ttgctatcaa
    17761 aaaaaatcgg aagttttcac actggttcag gtaaaaatat aggtttagga cttgcaggtc
    17821 taataaaagt atatctgaat ttttagcatt tcattacatt tgtaaaaaaa aagaatggac
    17881 tttaacggcg aggagcccaa catttttgaa ggtatatcaa ccgcaggaac aggcaacggc
    17941 gcaaaggttt acctggaaac atacggttgc cagatgaatg taaacgatag cgaggtagtt
    18001 gcctccattc tgctggcaaa cggatactcc ataacgcagg agataaacga agcggacatc
    18061 atacttatca atacctgttc aattagggaa aatgccgaaa ccagggtttt tggccgtatt
    18121 gacctgtttg gacaggtaaa gaaaaataaa ccatcggtaa ttataggcgt tttaggatgt
    18181 atggccgagc ggcttaagga tgagctactg gagaagaaga aaattgtgga tttggtggtt
    18241 ggcccagacg cctataggga gctgccaact ctgctacgag cggctgaaag tggacaaaag
    18301 ggggtaaaca ttatgctctc agccgatgaa acctatgcag acataagccc ggtgaggctc
    18361 gacaagtatg gcgtatcagc ctttgtatcc ataatgcggg ggtgcaacaa catgtgctcc
    18421 tactgtgtgg ttccctacac ccgaggagca gaacgcagcc gagatcccca aaccattatc
    18481 catgaggtag aagagctggt tagaaacggg taccgggagg ttacgctgct tggtcaaaat
    18541 gtcaactcat attcctggag cgaatcgaag gggatggtaa agcacaccgt taactttgcc
    18601 gaccttctgg agatggttgc cctggtgagt cctttcctaa gagtgcggtt ttccacttcc
    18661 catccaaaag acctgaccaa tgatgtgctt tacaccatgg caatgtacga gaatatttgt
    18721 aaccacatcc atctacctgt gcagtcaggc tcagaccgca ttctccagct gatgaatcgc
    18781 aagtactccc gcaacagcta cctaaacaga atacaggccg tgcgggaaat aattcccgac
    18841 tgtgctatat caaccgatat tattgcaggt ttttgcactg aaaccgagct cgatcacgac
    18901 ctcaccctaa gcctgatgag ggaggttggg tttgactttg cctacatgtt caagtactcc
    18961 caacgcccaa atacaaaagc ggccaggcat ttagttgatg atgtacccga tgaaattaaa
    19021 tcaaggcgct taaccgaaat catcgagcta caaggtaagc tgtcggaaga aagtaagaaa
    19081 aaggatgttg ggaaacgatt cgaggtgctg gttgaaggca cctcaaaaaa atcggacgat
    19141 ttctatgtgg gtcgcacaac acacaataaa gtggtggtgt ttccaaaaga agcaagtagt
    19201 attggcgatt tcgtgctggt tgaggttacc agctgcacgc cagctaccct aattgggaag
    19261 attgttgacg agtagtagta aaaaaggccg tataaaacgg ccttttttgg ttgaaataat
    19321 actctaacac tacatcagct tgttctgaaa ttcgctcttt aaatttcccc cctcatcaaa
    19381 ccactcggtt agcccgtaaa agtaatttgt actcccctcg ggtttgaact tcacaaccaa
    19441 cttaagattt tttgttaagc gggtaagatt tgtagccaca gccattctta ctctatccct
    19501 atccgactcg tcgataccta aacgctccat tgcctcaacg gtgagagagt ttgccagcat
    19561 caagttttgc ttgctacgaa gaatatcctg aattagatca atccatttta ccttgtcggg
    19621 accagcaccc gacttaatct tctttcgtac gacactttcc tttcggggcg atttcttagc
    19681 ggcaactttt gatttcaaat ttgcctttct cccccttttt gtagcagcca gcttggtttc
    19741 ttcggaggta gcagcattag ccttactttt ccttgtcttt ctaatggctt ttactttagg
    19801 ggcagcactc ttagcaacgg ctttcgcatt ccggcctttt cgaacttttc taccatcaac
    19861 cttggcctcg ggtgtaactg cttcaccggc tgctgggact gattcaacca attttttcct
    19921 ccctcttctt gctggagcag caacccggga ggcgggttcc gaaacttctt cttgtgcccg
    19981 atcaatcttt tttagaattg attttaaatt ttcaattcta cgttgagttc tgtcaatctc
    20041 ggtttggtag agatcaatca aatcgttaat ctctcctggg gttaaattta ttttactcat
    20101 cttttagtgc tattaaaaat taactgatta gttgaaaatt tcattctaaa actgttacca
    20161 ttactaaata aaataaaggt gaaaacaaaa aaatatctat aaaaagaaaa taataatttt
    20221 tataatttaa ctttttatca acaaatcaac tagttaataa gtttcaaaaa tagataaaaa
    20281 aatattatat aagtacaact tgtgtgattt tttttatttt aattatcaat gtttaattaa
    20341 atccctaatg ttgatgagct ctctcatcgt tttcaaaata accatttatg aaaggcgtat
    20401 gtagtgaaat tgatttacaa atatcaacga aattaatata tttagctgat tattaactca
    20461 tagcaacatt tttaatacat gacttcagag tagcgatttt atttttctac atgcaagcaa
    20521 ccatccaaag caatcagacg gtttaatatt agccaggttt aattgtgaag ttgaacttga
    20581 agaaaaatca cattaaaatt tttcaggata accctctaaa aaagggaagg aaggcaaaag
    20641 ccactttaca tgaaaattat taagtataaa aaacgcttaa actttggtag gcaagatata
    20701 cctgtggagt aggtgcccgt taaaccaaat gcaccaagac ttatcaaaga accgaaataa
    20761 aacacgagaa ctgaatgtgt tattcgagct agcctcacca tgtgtatgca ttttcccctg
    20821 ggactacaag gcttacctgt tcactttaaa tagtacagct aataccgaat taaaatattt
    20881 caaccgccat gcaaagcgca ctccagtttg tcaacataac ataaattttt aaaaaagtgg
    20941 taaattgtaa accatagtaa taactctatc agcctgaacc aagagcattt gcctaagcaa
    21001 aagtccgagg aaatgttgaa tgaagtaaat ttgggttaca aagaatcttc aagagatggc
    21061 tcgtttgtga gttttaccag caaagtcatt attttaaaac gacaaaatgc taatgattgc
    21121 aacaataagt aaactggtag gttggcttta ttttttcgga ctagttctct attcatatta
    21181 atactggcta ataaatataa agatgtaaag aatcatgatt aagcaggttg aaatccaaaa
    21241 cttcaaacct aggtgtttcc cggccaaact tcttatccaa ggcatccagc aagcatggcc
    21301 tgctaccatg gtaaatcagc atatatacca gcgaaataat aacccaataa aatttcaggt
    21361 ggaacattca tgcagggcag cttgcacaaa tgcgccggaa caaccaggcg atactagtaa
    21421 gttcggcgag gccaaaaaat gggaattcca tctgttcaac tccataccta aatggtatgg
    21481 ggcaaagtac ttgtaatcaa aaacctaaaa atttttcacg aatgaaaaga aaatctttca
    21541 aaacagctat gctcctggtc gccctaggaa gtagcttatg ggcacaaaat agttcaaatc
    21601 ctctatggat gcggtatcca gctatttcgc ccgatggcaa acacattgcc ttttcatata
    21661 tgggcaacct atttagggtt tcgagcgacg gcggaatggc agagcttctg acctccaatg
    21721 cagcgtatga tttcagcccg gtttggtcgc ccgatggtca aaccattgcc ttcgcatcaa
    21781 accggtatgg caattttgat gtattcacca tccctgccat gggaggagag gcaatgcggt
    21841 taacctttca ttcggctgat gaaattcctt ctgcattttc acccgatgga aaaagcatcc
    21901 tattctcagc gtgcatacag gacaacgcta acaacattca attcccttcg ggagccctat
    21961 cggagcttta ctcagttcca actcagggag gtcggatagc acaaattctc actactccag
    22021 ccgagcaggc cgtttacaac ccagagggga ctgaaattgc ctacaccgat ataaaaggtt
    22081 atgaggataa atggcgaaaa caccataaat cgtcagttac ccgtgatata tggatttatg
    22141 atattagcac cggaaagcat cgtaaggtaa ccacttttga gggcgaagac agaaacccgg
    22201 catttgctcc cgatggcaaa tcgcttttct acctctcaga aatgcagggc aactcaaacg
    22261 ttttctcggt tagcttatcc agtatagggc aaccagagtc cgtttcaagc ttcaccaacc
    22321 acccggtacg attcctttcg gtggcccgga atggaatgct ctgctaccag tacaacggag
    22381 agctatacac tcaaaagcca ggtagcagtc cgcaaaaggt caacattcaa atagctaccg
    22441 accaaaccga aacccgggta aagtatgaaa acttatccac cggcattacc gaaatgaacg
    22501 tttcgcccga tggtaaggag gtggctttta ttatcagagg tgatattttt gttaccgcta
    22561 ccgactaccc taccaccaaa aggataacca acacgcccga tcaggaacgt tcggtcagct
    22621 tcagcgccga tggtcggaag ctactatact cctcagaacg cgacggtagc tggaagatat
    22681 acgaatcgtc tattgcatca gccgaggaac caaacttctc actttccacc atcatcaaag
    22741 aacaggttat tgttgacata cccgaggaag cttttcagcc cctatactcg ccagatggga
    22801 aagaggttgc ttttcttaag gaaagaacga cgctttgcgt ggtaaacctc gaaacaaaaa
    22861 agattagaac cgtcctcgac ggaaagtata actattccta tagcgatggg gatcagtggt
    22921 tccaatgggc acccgacggg aattggctag ctgtaaactt catggaaacg ccttcgtggc
    22981 ccgattccga tattgccctt gtaaaggcag atggttcagg agaatttcac aacatcaccc
    23041 aaagcgggta cgacgaccga aaccccaagt ggaccatgga cggaaaggct atcatctggt
    23101 taaacgacaa aatggggatg agaagtcacg gctcatgggg ctcacaattc gacgtgtatt
    23161 ccgcttttct tacccgggag gcttacgatg agtttaaact gagcaagcag gagtacgaat
    23221 tgcttaaaga aaaacgaagt aaggataaaa aagacaatgg agcaggcgag gataaagaaa
    23281 gctcatccga taagaaaaag gaggtaaaag ctaaaaaaga caaggaagca caacgcaaac
    23341 ccctaatcat agactatgaa aacctcgatg accgcaaagt tcggctcaca ataaattcat
    23401 ccgacctctc cgatgcaatt ctcaccgatg atggggaaaa actctactac ctcagcaagt
    23461 ttgagaaagg atacgacctt tgggttaaca accttaagga taatgaaacc aagctgctgg
    23521 tgaaaattga tgggtttgcc ggcgatttgc aaattgataa ggataaaaaa ttcctgtttg
    23581 taaactccaa cggcacaccc atgaaaattg agattgccac caataagctt tcgccaataa
    23641 aatttaatgc ggagctggaa acaaactacc cccgtgagcg ggactacatg tttgagcaca
    23701 tgtggcggca ggtggaaaag aaattttacg accccaacct gcacggggtt gactggaaat
    23761 tctacaagag cgagtatgcc cggtttctcc cccacatcaa caacaacttt gattttgccg
    23821 agatgatgag cgagctgctt ggcgagctca acgcatccca taccggttgc aggtacaggc
    23881 ctaaaccaga gaatgcagat caaacagccg cactaggagt tttcttagat tataatttcg
    23941 atggaaaagg ggtgaaaatc atagagataa cggacaactc cccgctggtg aaagccaaca
    24001 gtaaagtaaa accgggttgc atagttgaaa agattgatgg cattgccatt gaatgcaact
    24061 ccgattacta caaaatgctc aaccacaagg ccaataagct aaccctactg agcttttata
    24121 atccccaaaa cggagaacgg tgggaagaat cggtaaaacc tatcagcagg tcaatagaat
    24181 cggagctgat atacaagcgt tgggtcagga aaaacaatgc caccgttgaa aaaatctcga
    24241 acggccgaat aggttatgtg catgtgaggg gaatggactc gcagagcttt agggaagttt
    24301 actccgacat actgggaaaa catcgtaaca aggaagcaat cattgttgat acccgattta
    24361 acggtggcgg gtggcttcac gacgacctgg ccaccctcct ctcagggaaa cggtatgttg
    24421 attttgttcc ccgaggccag ctaattggct cggaacccat atcaaaatgg tataagccat
    24481 ctgtggtcat cataagtgag ggtaactact ccgatgccca cggtttccct tacgtttata
    24541 aaactctagg cgttgggaag ctggttggta tgcctatagc cggaaccatg actgcagttt
    24601 ggtgggaatc gcaagttgac cccaccctgg tttttgggat tccacaaatg ggtgttaagg
    24661 atttaaacgg caagtatctg gaaaatcaac agctgaatcc cgatattaaa gttgccattt
    24721 caccggagga agcccagtcg ggcgtagata gccagctcgt aaaagcggtt gaagagctgc
    24781 ttttagagct caaccaaaaa taggactcaa taatgtcatg gaaatttttg tagtaaatga
    24841 tattatttat acctttgtaa ttgtacaaac taactaaatc tatactacta tgaaaaagat
    24901 tgctttattt gtaattgccg ccggaatgat ttccatggtt gcatgcaagt ctgctccaaa
    24961 acaggaagag gctactgaac aacctgctac tgaacaaact cagcaagaaa cagctcagcc
    25021 cgattctgca gcacaagctg ccgaaactcc tgctcaacct gctcctgcac agtagttgtt
    25081 gctataagga caaataaaga caccccatat ttttaatgtg gggtgtttta ttttttaaaa
    25141 aaagggtagt gccagtaaaa actactcctt aatgatgata ttcagctcat caagctgctg
    25201 ttggctaatg gatgaaggcg agtcaatcat cacatcccgg cccgcattat tcttcggaaa
    25261 ggcaatataa tccctgatgg aatcggagcc tccaaaaagg gagcagaggc gatcgaaccc
    25321 aaacgctatt cctccgtggg gcggagcgcc gtacctgaag gcatttaaaa gaaacccaaa
    25381 ttgtttttcc gcttcatcat cggaaaaacc caacgtgtta aacatggttt gctgcagctc
    25441 cctgttggaa attctaatgg atccaccacc tacctcaact ccgttgataa ccatatcata
    25501 ggcgttggcc cgtatggccg caggatttga ttggaataag gggatatcat cgggatgggg
    25561 cgaagtaaag ggatggtgca tggcaaaaaa ccgctgtaac tcctcgtccc attcgagtaa
    25621 agggaagtca actacccata ggggagcaaa ctgattttta gggcgtaacc ccaagcggtt
    25681 tcccatttcg agccttaact cgctaagtgc aggaagggtt ttgttgcgct tgcctgccaa
    25741 aatcagcatc aagtcgccag gtttggcttc aaacactttg gcaaccagca tcaaatcgtc
    25801 ggcagagtaa aacttgtcaa ccgacgattt cagtgtccca tcatcgttgt agcgaatata
    25861 aaccaaaccg ctggctccaa cctgtggccg tttgacaaag tcggtcagct catcgagctg
    25921 ttttcgggtg tagctcgcac atccccgggc gcagattcct cccacatact ccgcattatc
    25981 cacaaccgaa aagcccttgc ctttaagctg tgcaccaata tccttgaagg acatgccaaa
    26041 tctcagatcg ggcttgtcgg ttccgtagag cgacatggcc tcgtggtagg tcattcggcg
    26101 aaagggagcg tcgaattcta cccccttaac agtatggaaa agatgcctgg ccattccttc
    26161 aaagacatct agtatatcgt ctctttcgac aaacgacatt tcacaatcaa tctgggtaaa
    26221 ctccggctgc ctatcagctc tcaagtcctc atccctaaag caccttacaa gttggaaata
    26281 cctgtcgaaa ccagcaacca tgagaagctg cttgaaggtt tggggggatt ggggaagtgc
    26341 ataaaactgc ccgtggtgca aacgtgaggg cacaacaaaa tcgcgtgccc cttcgggtgt
    26401 tgatttgatt agaaaagggg tttccacctc aagaaatccc ttattgcaaa ggtatctccg
    26461 aacctcctgg gccatggtat gccgcaacat cagggcgttc ttaacgggat ttcttcgtaa
    26521 gtcgagatac cggtacctca tccgtagctc gtcgccccca tcggtatcat cctcgatggt
    26581 gaacggtggt aattccgact ggttcaaaat ggtaatattg ctggtgcgca gctcaacctc
    26641 accggtagga ttttttggat ttttacttgt tcgctcaacc acctctcccc taacctgaac
    26701 cacaaactcc cttcccagct gttcaacaat tgaatgtaat tcgttactgc ttttttcgtc
    26761 aataacaacc tgggttactc catacctgtc gcgaatatca ataaaggtca ttcccccaag
    26821 gttacggatc cgctgcaccc aaccacaaat cgtaacaacc ttttggacat cgctaattct
    26881 gagttctcca cacgtgtgtg ttcttaacat acggatattt tttaacctgc gaatgtaaaa
    26941 ataaaaaatg acccatacgc atagaattgc tatttttgaa gttgacaggt atccctaaaa
    27001 tcctttgaca atgaacttat actcccacga gtattttatg aacgaagcgc ttaaagaggc
    27061 caaagtggca cttgagctga aagaggttcc cattggggcg gttgttgtgt gcaacaacag
    27121 aattatttcc cgggcccaca acctgaccga aacccttaac gatccaaccg ctcatgccga
    27181 aatgcaggcc attactgccg ctacaaccta tttaaatggc aaatatttag accaatgctc
    27241 cctttacgtt acccttgaac cctgccccat gtgcgccgga gccatgtttt gggcacaact
    27301 tggcaagctg gtgtatggtg cttccgaccc caaaaggggc tatagcctcc acggaaattc
    27361 aattttacat ccaaaaactc ttgtagttgg tagtattctt accaacgagt gctctggcat
    27421 cctggtcgat ttttttagag gtagaaggta gccacagccc ctgctgttgt tattcctacc
    27481 taatttcacc ggtaacaatg cctctaccct tcgcaccatc attcaacaca attccactgc
    27541 acttcccatt ttgcatgaag gtatttaaaa ggaaaccttc tcaatgctta tgcccgattg
    27601 ataacccgaa taattttcat taaaagttcc tgagtataaa caagcactaa gttgctctaa
    27661 atgttttaca tttgcatgat ttttccctat aaatagcagc ccaacttcac gggtctatgt
    27721 accaacctgc tcaaccaaaa atagtatttt cagatattga tggaaccctg ctgaacggta
    27781 gcaggcagct ttccgcaact accattcaaa ccatcaggaa aattacagct gcctatgacg
    27841 tttactttat actagtctct gccagaatgc ccatggcaat gcaggctatc tatgatgagc
    27901 tctgcctaaa aacacccatt atttgctata atggcgccct tataatggaa agcctagctg
    27961 gtggtttcga caatcaaagg gaaatatgct cccactccat tgacccccgt attccgctat
    28021 caatatttaa ccacacgctg gagcataacc tgcacttcgg gttatacagc aataacagct
    28081 ggtttgtctc aaaaaatgat gtgtggacag aacgtgagga aaacaacact tgtgttaaag
    28141 cggttatagc caacaacatg ccaggtataa tatcacagct ggccagtgcc ggtgagccca
    28201 tacataagct tatggtcatg ggaagccctg ttgaaatcga cttgctcata aatagtattt
    28261 ccctggaaag caggaacaaa attaccattt acaggtcgaa ggaaaccttc ctggaaatat
    28321 caccagcaca atcgagtaag gccaaggcct gctccttact tctcgattac ctaaacctta
    28381 aacccgagca ggcagtagcc tttggtgata attttaatga tatggaaatg ctcaggatag
    28441 tcggaacagg ggttgctatg ggaaatgcaa gtgaggaggt aaagcgacat gccgacaaaa
    28501 ttgcaccgcc aaatacggag gatggggttg cctttgtgct ggcagaattc ttcggaatat
    28561 gacctcattg gaacttctat caccggcaaa agaccttaaa acaggaatgg cagccattac
    28621 ccatggtgcc gatgctgttt acattgcagc ataccgcttt ggcgctcgcg aaaaagccgg
    28681 caactcaatt gaagatattg gtaagctaac agaatttgcc caccagtact ttgccagagt
    28741 ttatgtaaca gtaaacacca taattttcga caacgagctg gacgaagtcc aaaacctaat
    28801 taatgaactc taccgggttg gggtggatgc cattattgtt caggacttgg ccattctttc
    28861 catgaacatt cctcccattg ccctgcacgc cagcacccag atgcatacca atactctggc
    28921 taaactaaag tttctggagc agtgtggttt ccaacgggta gttttaccac gtgagctaac
    28981 tctttccgaa ataggcagct attgcggctc aacctccatg gagattgagg cttttattca
    29041 cggtgcgctt tgtgtttcct atagtggaca atgctatatg agccagagaa ttaccggccg
    29101 aagcgcaaat cgtggtgagt gcgctcaacc atgcaggtcg tcatacgacc tggttgatga
    29161 gcatggcaaa attctggtca ggaataaaca cctgctatca cttaaggatt tgaacctgac
    29221 aagccaaatc gagcagctgg ttgatgccgg ggttacctcc tttaaaattg agggccgcct
    29281 gaaggatatt acctacgtta aaaacatcac cgcccactac aacaaaatac ttaactccat
    29341 tatcagccga agaccagaat ttaggagagc ttcttcgggc gtgtgcgagt gcagcttcga
    29401 tgccgatccc gagaaaacat tcaacagggg ctacactcag catttcatca acggtcgaac
    29461 ggttaatcaa acttcattcc acactcagaa atcggtaggt aaacccattg ggatagtagc
    29521 acgggtaaat gctgattatt ttgaaattaa cagctacgaa gctgttcatc ccggggatgg
    29581 tttatgctac ttcaccacca tcggccagct tagcggtatg ctggtaaaca gggtaatggg
    29641 caaccgtatt tatccacgta acaaccccac agggctaagc gagggtgata ccatttaccg
    29701 aaattcagac atcgaatttg aaaagcagct taacggaaaa agcgccaggc gatgggttgg
    29761 agtgcgcata gctgtaagcg acaacaacgg caaccttaag ttcagcgcca ccgattccga
    29821 tggtattgct gttgaatata ccctcaacaa caactttgac gaggcaaaag acaaggcaag
    29881 gtcagtggaa atgctcatcc gtcaactctc caaatcgggc gattcgattt ttagggttga
    29941 tggggttgcg gtgcaggact acactactcc aaaatttatt cctatctcca cggtaaatga
    30001 ggccagacga atgctactaa acaaccttat cttagaaaga ttaaagcagc acacccggcc
    30061 ctttcggggt aaatattttc ccgccgtttt ataccccgag caagccatta cctacaaggg
    30121 gaacatttca aatacagcca gccaggattt tttactgaaa cacggtgtaa cctcaatagt
    30181 tgaagctgtt gaaatctctg ctcccaaggg agtcttcgag ttgatggtta cccgatactg
    30241 catcaaattc gagcttgggc tttgcccatc gaagcagaaa gcttcaccca cagggcagct
    30301 ctatctccaa aacagctcaa cgctctaccc cctggttttc gattgtaacg agtgccagat
    30361 gaaggtgatg agtcccaaat agccatagaa cttattgtgg aagaaatgaa acaccaatga
    30421 agggcggctt gtataaatac gcttgaacaa ccgggcgtta cttacgggtt tggcgaagcc
    30481 aaaatattgg cgttccatac gctttacctc gatacctata aggaataaca taatgtattt
    30541 gtaatcagaa acctaaacat tttatactga ttaaaattct taaaaaatct gcataaaact
    30601 gaaaaaacag gtattttcga taaagtttct gattcgggaa gtgctaaagc atatacccgc
    30661 cacagcttaa aaagattgca gatgattcaa cttttaactc ccgaaagctg gccagagtac
    30721 gagcttattg acagtggtga tggtgaaaag ctggagagaa ttgggtcgca aatggttgcc
    30781 aggcccgaac cacaagcgtt atggaggaag caattatcaa atgaagaatg ggtcaagctt
    30841 gccgatgcgt attttgttaa aagcaaaaaa accaaaccga ccgaaaatga gtggggtgaa
    30901 tggcgcctaa agtcgggggc aaaagagcaa tgggtggttt catataacca gagcggcctg
    30961 gcatttcgcc tacggttggg attaacctcg tttaaacacg taggcatttt ccctgagcag
    31021 gcggttagct ggaattatat ctacaacact attaagcagc tcgatttggt tgaaaccaag
    31081 gtgctaaacc tatttgccta tacgggggca tcgtcgctgg ctgccagagc agcagaagca
    31141 aaagtaaccc acgttgactc ggtaaagcag gttgtttcat gggccaatga gaatatgcta
    31201 ctttcaggaa tggataacat ccgttggatt gtggatgatg ccttgaaatt tgtaagccgt
    31261 gaagtcaggc ggggtaacct ctacaacgga attatactcg atcctccagc ctacggcagg
    31321 ggccccgatg gagagaagtg gacagtagag gagaacctct accaacttct cgagctgtgc
    31381 aagcaggtaa tggaacctaa aaacaacttc cttgttctca acctatactc aatgggcttt
    31441 tcacccttaa tagccgaaaa cctgatacgg gacattttcg gcgttcccag ctatgaggtg
    31501 ggcgaacttt ttgtttccga cagggcaggc cgcaagctgc cgcttggaat ttttgtcaga
    31561 tttcgctctg gtagtaattt ttaacttgct gattggttaa ttgttagccc attttaaagg
    31621 tgttaaaaaa aatgatggtg aatttagtaa ggtgttttta ttacctttgt caagattttg
    31681 tatatatttg attatcaatt gtacttccaa caggattagg ttttaaaatt ggagagctta
    31741 tggaagcaat aaaaatacag ggaaccagcc aatatcctac tattgtgctc gacagtgaag
    31801 caggaaggtt tgaattctca gggaactctt tgcccgagga tccaaaagcg ttttacgagc
    31861 caattcttag ctggctcgac gcatacgccc agaaccccaa tcccgaaaca gttgttcgct
    31921 ttaagatggt ttactataat acccccagct caaagcagat tttccagatt tttaagaaat
    31981 ttgaaaaaat ggctgcttcg gggcataagg tttcggtggt ttgggcctac aacgaggatg
    32041 atattgacat caaggatgcc ggaaaagacc tctccgacca cataaaactc ccattccggc
    32101 ttgagtccta caaggaataa aaaaaggcct ttcaggcctt tcttttttta gccttcaagt
    32161 cttctaccaa tcagtagtgt agctatggtt gtatatcctg taagttttag actcaccggt
    32221 gttgtttgac ctgtagtaga tgtagcccga aattaacgaa agagttttac ctgccgagga
    32281 tgtcccggca atcttaatgg gctgttcaac attcaacttg aggttgtggt tgccctttcc
    32341 ctgaaaactc tggccaatgt tatcaataac aacaggagga atccctttcc actcaatcac
    32401 gaccttatca atggtaaagg gagaatcgta cttcgaacga accgtcatgt ccagcagagc
    32461 gctaaccaca ttagtgctat cttttgatat ggcagtaaag ttgaagggat cgctcacaaa
    32521 tattgaagcc ttgttggcag caacatcaaa gtagaagggt ttttgaacgt cggccactcc
    32581 aaatctgtac tttaccagaa tgttgttttc cggatcctcg tagcagttat cgtagtcgta
    32641 cttgatggtt acgttatcaa cattgttaac aattgaggta acccttgcgc attccgattt
    32701 aacaacaggt ttttttgttg ttgaaatcga acgccctccg agtcggaggt agtacttaag
    32761 ctctcccagg ctgctctcca gctcaaccaa ctcaatggag ttaagcagcg agcggtacct
    32821 ggtgatccct aggttgcact ggtcaaccct ggcatccatg aagtactcct ttagacttgc
    32881 aagttcggcc atgttctttt caatttgctc aaccgattca acctggtcaa cctgggctag
    32941 caaatcttcc agctgtcggg tcaactcgtt atcgcgcatt ttgaaatcgc ttaccagctt
    33001 catcagctca aactcaggaa aagggtactt aatgtgatag gtataaatta tgcttttgtc
    33061 gggacgctgg agcttctccc agtagaagtc ttcaaccttt gatagcgaaa tgccctgcag
    33121 gaaaggcacc ttacccgact gggccgttgt ggtggaggca aacttttcga ggaatatatt
    33181 cacattattg ttgtagttag cctcttcctt ctgaatggtg gaggtagatt taacattttg
    33241 tgcaaccgac gtaacgatac tctccttaat ggaggttaaa gcattctgct gcgcaacctg
    33301 caaatcgggg ccactgccgg caacaattat gtaatccttc tctataccat taacccactt
    33361 gggtttcttt ccgcttttat ccaacacctt atcctgcgaa aaggtatatc ctgccaggca
    33421 aaccatcgcg gcggtcagca aaagaatttt tctcatagct aattaaattt ccccatcgta
    33481 aagaatggga gattataaca attcaagcac ttaccataag taatcagcaa ccccgatggg
    33541 ggttgctgaa ttcttgggtt atcaaattaa gggcgttcgc tttccagctt gttcatctcc
    33601 tcgttgaaga tctgctcgaa tttcatcttg tcgtagtcga gctgcagctt ctgatccttc
    33661 gaaatccggt tggaaatgtt gttcagcacc tcatccttgg aaacctggag cgcggtgaaa
    33721 acctggaact ttccatcctt ggtttgcatg gttttattgc aagctacctc aacgttgttg
    33781 agctgctggt ttacaacctc acgcgacatt tgctcgaact tctgctcaaa ttctgaattc
    33841 tcgccaattt ggcgctcgtt aacatacctg tcagataccg acttaatggt cgtgtttata
    33901 ctgcgagcca ggttagtatt ggcatcgagc cttgccttac tcttggcagt gcttaggtcc
    33961 tggctgatgc cattacctgt tccacggaaa aacttggcat cggaatgaaa ctcgtcatca
    34021 ttgcagggaa ctgaaacttc cttcccgaat gaagaatctg cagccttttt ggaaccaccg
    34081 cagctagata aaacagctgc aaaggtaaaa gcagcagcaa ccataatcat acttttcatt
    34141 ttcatagctt tcaatttaaa gttaaacttc ggattaagtt aaaataatgg gtagaaaaat
    34201 tttttgctag ttgtgaatta ctttttaacg taaagccagc tccaatccca gttgaagtag
    34261 gaggcagcgc cccagtaagc ccaagactgg gctacctcct tgcctgcctg tatcatcatc
    34321 ttgtccttct caatggaatc gtcgtactgc ttcatcttga actcccaaac gttggcgcca
    34381 tcggcaagct gcttctgggt tatctcatca gccagcttta ccgcttcgct atagcactcc
    34441 atgtcggggg taattttccc caggtactcg gcagccttgt caacgttcat gttggcccac
    34501 gcagcttttg ctccagccat gtactccgtg cacttgtagt tggcgtactg cttgtatatc
    34561 tctacggata aatccatact cttgtcgaag cactccatgc aaacatccgg aacggagagc
    34621 aggatgtaga gggcctcctc gtatttcttc tgaccggcaa gggcctgggc gcctttcagc
    34681 accacgtcgc actgcgagtt gtagtactct attatctttt cctttccctt ttcaacaaaa
    34741 cccttgaact gccctgcttt tacgttgact cccttcagcg cttgaatgta ggccttatcc
    34801 tccgtctgcc caacaccctt aacctgaatg ctggtctggc tgaagatgtt ctggcttgca
    34861 gcgtcaacaa tgtagaaggt tacttcaagg tttacagcct gcattggcgg tgctgttggg
    34921 gttatctcct tgcttaacac gtttaccata ggcacaatgc agaaccgggg gtttgcagcc
    34981 gctgcgccca gcccattctg cacggctatt tgctccatct tatttaccag ctgctggcga
    35041 gccgattggg gaatgccctc ggcctgctcg ggaactatcg ctgcaagggc aatgcgcccc
    35101 aagtcgtcgg ttttaccctg actattttgg gcacccaatc caacggtaaa tccaaggatg
    35161 gtagcaacaa gaatcaatat tcgtctagtc atcacctttt attttctgat taatagcatt
    35221 ttacttttca ccaaggatta gccaggcctc tcctagtcct ctcaggtata actttgaagg
    35281 aatgcccatt ccgttgaggt acttgcgcaa gtcgcttacg aacgaacggg tgtcaaccgc
    35341 acgctcccga cccttatcgt cagtacgcat catgggaatg cgcacctgct caaacttcat
    35401 gaaattttcg gtagcatcgg aaaggctgaa gcggccctga acgcagttgt cggcaaacca
    35461 gttctcgatg atggttgaaa gctcctcgga gttgtcgcca aattccgatt cgaggttttc
    35521 accccagttg ctccatacct taatctgaac cttcacctcg cgaccatttg caaacatatc
    35581 ctcaaaatgc ctcatcagac ctgcattaaa gttgtccatg tggctgagaa cagcttcctc
    35641 caggagaagc tcgggtgagg cagccgaaga agggttaccg gcacctgcca cgcccgatac
    35701 ctgtttggcg gtgtaggcat caaggccttt aaggttgaaa acgatgtact tttggggtcc
    35761 ctgccttttc acctcgaagt cgaggtccat gatgatgtcg gcttttgcaa cacgctttaa
    35821 tgcgtcaata gggctttcgg ccatttcgga gcctgaagat gagctggtaa gcatggatga
    35881 ttctgctctt tcttgttcca aattcttcat ctcctgctcc agatccttaa gcggaaatcc
    35941 cctgtcggcc ataatctgtc ccattttggt aattaccaac cgtaaatcgg gatcggtttg
    36001 cagggcagtt ttatagtcgg gaagggtttg cttcattccc tggttatcaa acaccatctt
    36061 gtagccccgg cttatgcagt actggtcgct gggcactacc ataataacgg gtttctttgc
    36121 ctgaccaaac accgccaggg tagaaaaaat cagaattgat gaaaaaagta gcttcttcat
    36181 ggtaggttaa tagttagaag ttaaaatccg gaatccaacc tgcgggcaat gccatcggcc
    36241 tcaagctgct tgcgtagggc atcgtacatc acctgcacgt atatggccac cttgtagccc
    36301 tttttaattt ttatacgatc ctcgcccgat ggatcgccat cgttggtaac ggcaatgtac
    36361 tggaggtact tgccacctac ctcaaaaaac ttttcgaagt agtcggcatg ggtaacctcg
    36421 gcgttgggtt gagagcatat ggggggagtt ggagcagatc cggctccacc gggtaaaccc
    36481 ttaaaaatac aggcagcaac ggcagcttcc ttggcattca taactgcagc acccacatcc
    36541 ttaccgtagc cccacacctt aaataccttt gtgccatcct ggccaacccc aaccggctca
    36601 atttcatagt tgtactggct gttgtagagt attttgcgtt cttttctagt ttgggcattg
    36661 gcggggaaaa ctataaccca tgccaagacc actgctaaca ttgtggttaa tttacctttc
    36721 atagcttaat atacttttag tttagaacat aattttaaca cctaataggg tcgaaactcc
    36781 accgcggtcg gggaagacct catcccaggt aacccccaaa tcgccattat catttgatgc
    36841 gttgctgata aaggagtatg tgcctatgcc acccatcagc tgtatgttat gtttcaaatt
    36901 tagtgccaaa tttcctccca gcctcaggta aagggtggta taatttccag tattagcagc
    36961 ctcaatggca tcgctggtag cagactcctg accaatacca acgtatggac gcaattccac
    37021 gttgcgggtg agcatgaacc ccttggcaat acccgctccg tagtgggtaa acgaaacatc
    37081 ctcggttgct agggataaaa tatcatactc cttagtctgg aatccaactt caccaaatac
    37141 aaacagggac cttattccaa caaaccttcc aagcctgaag tcagccctac cgtaaacacc
    37201 gccaacttca cccatttcaa atccggcaag cacctccaat cccatgtcgt tttgctggcg
    37261 aaccaggtag ccagtttgga gcttacgccc agcagtttgg tagaatgtgg aggtgggcat
    37321 ttcgccggta gccacctggc ggttgtcaac aattttcgat gtagccctga taactcccct
    37381 gaacctgggt tcggtggtat tggttttctc gttgtaaaca tactcgtagg caaagtagcg
    37441 gtggtcgcat ttcagcccct ccttcttccc aattttaacg cggatagggt taacctgatg
    37501 aatggtggtt ttcaccataa agtcctcgtg gctcttttcc agattgtaaa tggtttcgtc
    37561 ataacctttc tgcaccagct cctgcaggag cacatcgtca gatttttgct taataaaccg
    37621 gccaatctgt gtgtcggggt taggctgaga tgctacgata ggtacgctgg ttttggttac
    37681 aaactcaagt ggaatttcga gctgctcgaa cttcttaatt ttttcggcct taacggcagg
    37741 gctatcctca ggatagatcc agcagtcgta tagggcattc tgggtttcct ccgtatagtt
    37801 aattttaaaa agataaccca ctgccgttgc ctgccaccca tgcatgttgt ttatctttgc
    37861 ctcctgcatg gttttcacat tttggtaatc aagcaccata atgtagcttc ggccaactag
    37921 ccggctaccg taatcctgaa gcacagcgtt tccaagcttg gttgattgag ccttaatgta
    37981 ggcagcatcg gtggcgttga acatgcccct gtcaaaaatc ttatccattg acatggaccc
    38041 atcctcctgc ctgttgtacc ataggctgat aattgacttg gcaacctttt ggtcggtcag
    38101 atacttctta acagcctcct gcggaacaac ttcagttgaa gtgcgcgaga atggagcatc
    38161 gaagctaagg gtctccatgt tgttattgta aaacttgtca gaaaaaacaa tggatccaat
    38221 tttcgactca acctcctgca catggttttc ccccggaacc ttcatgtaaa gaaaggtgat
    38281 ggagctgcgg tcgtaggttg agggaatttt ttttggttgc tcggcgttct gagcttttgc
    38341 tcccaacacg gccataatta gcatggcatt tacgaaaagt agtttaattt tcataggtaa
    38401 gtggttttgg ttttagactt taagtttaaa ttatttcaga aaaaatttag aaactaaaat
    38461 tatgccgatt aatacactaa agcaaataaa agtgattcca ccggaatact ttagttgttc
    38521 aaatatacca attaacctac tacccatttt cattcgtatt gccttgaatg gtgttcatca
    38581 aaaaaaaggg tctccccttt tttctacaat tccgccgctt taagtattag tttgggaata
    38641 tcggtgttat ccagtaatcc cggaagttgc gatgatcccc gacccattat aaaaaccggc
    38701 acaggcgagc ccgtgtggga gtggcttgtc caaccaattc ctgcttttct gttgagtata
    38761 gaaatggcat cacctgcaag taatccagcg tttttttgcg cattttttga ttcccctgca
    38821 tttaggttga ctaagcgctt ataggcagct tcaagggtgt tcctttcatc agcagtcaac
    38881 gcaaatgcac cattttctga aagcccgatc ctggcctcca gcaggccaag gtaatcctcg
    38941 aaggtggctt tagaattttt gctccataaa tcgtaaagtt cggcttgaag cctgtcggcc
    39001 gatgcctttt gaaggcttag cgctgtcagg tgcaaatcgt agccattggc attatttcct
    39061 aacgacattc cgcctgtttc gtgatcggca gtaacaataa tgagggtttc cgaaggatgc
    39121 ttgctgtaaa aatccaacgc cacgttcact gccctggata gagcaagcac ctcgtggatt
    39181 gcggtagcag catcgttgtc gtggcaagcc cagtcaattt taccaccctc taccatcata
    39241 aaaaagccct tagggttatc aaccacttca attgccttgc gggtaaggtc agccagtgaa
    39301 atgtccgagg ttttacgatc tatctcgtac tgaagtgttt gctccggtgc ggcatttggc
    39361 gttgagtaaa caattgattt tacgcccagc attttaaggg aatctacacc ggtaaaagaa
    39421 gaggtaaagc catagcccgc cctggttccc aactcataaa tagaagcaac agtgctccct
    39481 tggtgtttgg agccatttgg ttcaaggaaa ccaccggagc cataaaagtt atacccgctt
    39541 ttgagcaaat ccaaggcaat ttggtagtag ctgcttcggg agcctgcatg tgcataaaag
    39601 gcagcaggtg tggcgtgatc tatgctaaca ctggtaagaa tagcaacctt acgaccgtta
    39661 tccctgaacc tggttgcaat gctataaagg gtgtcggtat ggtttgcatt cagccctaac
    39721 gtcccgatag atgttttata ccccgttgat agggcagttc cggctgctgc ggagcaggtt
    39781 acgtatcggt tggcagcata ggttgttgca agggttgtga cagggaattg catgaaggta
    39841 acccccagcg tatcgtgtag cacgctgtcg gcatatacct gggcagcatt aacttgctga
    39901 atgcccattc catcgccaat aaacaaaaat acgtatttcg cccctttctg attctgggca
    39961 gaaataagag acggaaatat gaacagaatt aaccaaatag gcagtaagta tcgtcttttc
    40021 atgatagttt agattagtat tttaaacaaa attagtcgga attattaaca aaactattag
    40081 ctataagcta ttatgatgtt aaagttgatt aacaagaatt gctattgtga ggcttgctag
    40141 cctaatcggt ttgtatagta aacagctcca ccacagcgga gattacaagt tgcaaaatat
    40201 gccgattgga ttgctacccg gaaggtgatt gaaatattcg aggttattcc ctggaaattt
    40261 gaacttagga aggataatgg tataggcaga gcaaccaggg aattaattta ctgacaaaac
    40321 ttgaagctaa atcaaaattg gaaaggagaa ttttagcgaa ataatcttta tgaatcctta
    40381 aaaaaaaatt gaagaacctt aaaatcgata tggaatatca gaagctagaa cacgaaaagt
    40441 ttctataaaa aagatgagga tatccaaaaa aattgcggag aaagtgggat tcgaacccac
    40501 gttacgcttt tggcgtaaac acgctctcca ggcgtgcgcc ttagaccact cggccatctc
    40561 tccggtaaaa ccaattcaac taactcgttt aaaatcggcc gcaagataca aaaatattta
    40621 ctaaagctac atagctagta aagagtaatc tcgcctgcaa ttggattggc aaattccaaa
    40681 gccaaggcac tccctacaaa tccctttcct aaaaggtaca ttaacttggc tactgctgcc
    40741 tcaaaggtaa tatcgaagcc gcttgcaacg cctgcatttt taaggattaa tccattctcg
    40801 tatttgttca tattaacact tcctgcctta cactgggtaa cattgagaat agttaggcca
    40861 ttcgcaacag ccttagatat catttcaata aaccattgct ggctaagcgc atttcccgat
    40921 ccgtaggttt cgagaattac cgccttaacc cctggtgcgc ttaaagcaga ggcaaccact
    40981 tgaggagtaa taccgggaaa gagcttaata acccctatgg agctgtccat gctggtgtgc
    41041 accacaagcg aagaatttgc ccggggatag tgtatgaaag gatagttgta tttgatggta
    41101 atgcccgcct cggcgagcaa ggggtagtta tcggaacgga aggcgttgaa gtgctcggag
    41161 ttgtgcttgg tggttcgatt gcccctgtac aggttattct caaaaaaaat ggtaacttcc
    41221 ggtacaacag gcagcccgtc acgcttagct gcagcaattt caatagcgct gatgaggttt
    41281 tcctttccgt ctgtccggag cattcctatt gggagttgag aaccggtaag aacaaccggc
    41341 ttggcaagcc cttcaagcat gaagcttaat gctgatgcgg taaacgacat ggtatcggtt
    41401 ccatgaagaa taacaaagcc atcatacttt tcgtagttgc tgcttataag ttcggcaagc
    41461 ttaacccaaa aatcgggtgt aatatcggaa gaatcgatag gtggatcaaa ggagatggtt
    41521 tgtaggttga agccaaactt ctgcagctca ggcacctcct cctcgatatt atcgaagtta
    41581 aatggcgaaa gggctcccgt atgagggttc tccttcattc caatggtgcc gccggtgtaa
    41641 atgatcagga tggaaagact gctacgattc ttcattattt ataggcaaat taaacagttt
    41701 tatggcgtta gccgatgtta gcgaggcaac ctcatgcacc ggcatttcca ggatgccggc
    41761 aacccgctgc gcaatgaagg tgaggttgaa actctcgttt cgtttgcccc tagctggtgc
    41821 aggtgaaagg tatggggaat cggtttccag aactatcttt tcaatattta tatggggtag
    41881 caccctatca accccgccat tcttgaaggt taccactccg ccgattccca ccatgaaggt
    41941 ttggtagtcc agtatatgcc tgtagtcctc aagcgtaccg gtgaagctgt gaaatacccc
    42001 cgaaagcgtg gaatcggcct cctcatcgag aatgctaaaa atttcctgaa acgaattcct
    42061 ggcatgaata gctactggca atcccagctt ctttgccagc tggacctggt gtcgaaaggc
    42121 aaccttttgc tcctcaacaa aggttttgtc ccagtaaagg tcaatgccaa tttcgccaat
    42181 tccccaaaac tttcgaatac taagatatga gccaactacc tcaagttctt ttgaaaagtt
    42241 acggttgacc gaggtgggat gtaaacccat caaaggaaaa cactgggaat acctatccgc
    42301 agtttctaaa agtcgcggta cagaagtaga gtcaatattg ggtagcacat ggtacttcac
    42361 cccggcctgt gcagccctgt cgagcacaac ctccctatcg tagtcgaaat cctcggcaaa
    42421 aatgtgtgaa tgcgaatcta ctaaaagcat gctgcaagat gaagataggt ttaaaagcaa
    42481 agttaagcaa aattcgcatg ttcaaaattg gcaacctgcc tactccaaaa tcaggatcat
    42541 gttaaatttt accttgatgc ccgtaggctg tccccagcag gggttatgct gtcgagctgg
    42601 taaattacag gtgtaatggc ctgcctgaac ttttccaagt tttctggacg aatgggctcc
    42661 accggcggtg atttaacctt cagcggatca acaggtttac cattcatcca gaaacgaaga
    42721 tcgagatgtg gaccggtggc gtagcctgtg cttcctacat atcctatgat atcaccctgt
    42781 tttacccgaa caccctcggc tatacccttg ccaaatctgg aaaggtgatt atagcctgtg
    42841 gtgtaaacgc tattgtgcct gattttgatg tagttaccgg ccgattcgtt gtaacccttg
    42901 tctattacaa ccccgtcgcc gatagccata acaggtgttc cggcaggtgc ggcatagtca
    42961 accccactat gtggacggta aattctcaga acagggtgaa agcgccgacc gctgaagcgg
    43021 ctcgatattc tggaaaagcg caagggtgct ttcaggaatg atttgcgaag agaatttccc
    43081 ttttcgtccc agtagctggc aacggaatcc tgttcatacc tgaaggcata aaacttttca
    43141 ccccgatgct cgaaccatgc agcatgaata gttcccaggc caaccggcag gctgtcaaca
    43201 taaagctcat cgtatatcac cttaaactta tcctcggggt agagtccaaa gaaatcaaca
    43261 gtccatgcaa aaacatccga aagctccagt gccagcatgg ggttgatgtt tttctgcttc
    43321 atggtctccc acaacgagga ggtaatcgca gactcgtcaa ccctcctaac ggtggtaact
    43381 tcctttttga aagggtatat cgccagcgtg tcggaaatgt caaaaataac aaaatcaacg
    43441 ggtgagtctt cataaaccag gtatctaacc gcagcaagcg tatcgcgggt tctgaaaact
    43501 tcgtaatgat tgcctgcctt aaacttatgg ggatcaaaac tatcggcgct taccaaagtc
    43561 aagctgttta caacttcggg atctacattc aaatcgagca aaagattccc aatggtttgc
    43621 ccataccgca acgaaccttt ttcaatgtag aatgaatcaa caggaattcc atattttaag
    43681 atgggcttgg ccaaaagcgt atctttagaa tattcctcag aatttttact cgtccttacg
    43741 ggtgatatcc ccacaaaata atcaacaaaa aaaatggcta gtaatgccag aattactgaa
    43801 aaaaatgctc ttttcaacat aatatacaaa attctaaaca accagtaggc gaaaattctg
    43861 caacccaact cctttgaaag aactgctata ggagaaatga tgggcgtgtt aaaacagcct
    43921 caaagctacc aaaaagaaag gttaagaagg ggaaataatt tttaaatttc gtgtagcttt
    43981 gccaaaaatt ggtggtatga gcaatcaaga aaggaaaaca gagctttacg aattaggcga
    44041 attcaggctt attgacaggc taacggggtc atttggcaag cttcacgaaa ccaccatcat
    44101 gggagtggga gacgatgcgg ctgtggttga cgttggttct aaatacatgg ttataaccac
    44161 cgacctactg ctggaaggga tacacttcga cctaacctac tttccgctta agcatttagg
    44221 ctacaaggct gttgtggtga acctaagcga tgtgtacgcc atgaatgcta cacctaccca
    44281 aattaccgtt tcaattggcg tatcgaaaag atttggcgtg gagaatttgg aagagctcta
    44341 tgcaggggtg aagatggctt gcgaaaggta tggagttgac atggtgggag gcgatacctc
    44401 atcgtccatg acagggctta ccattagcat taccgccctt ggcgaagtct ccaaaaccga
    44461 cataacctac cgcagaggcg caaaggctaa cgacttgatt tgtgtaacgg gtaatctagg
    44521 ggcagcctac atgggcttac agctgcttga acgcgaaaag cgtgtgtttg aatcaagccc
    44581 cggggcgaaa cccaagctgg agggcaacag ctacatcctt gagcgaatgc tcaaacccga
    44641 agttcgtaag gataccctcc agaaacttag ggacaataag attaagccaa ccgccatgat
    44701 tgacatttcc gatggactat cgtcggattt actgcacatc tgcaagaact caggagtggg
    44761 ctgcaaaatt tacgccgata aaattcctat tgatagccaa acagcacaaa tggctgaaga
    44821 atttggcatt gagccgctgg ttgctgcgct gaacggaggc gaggactacg agctactctt
    44881 caccattccg ctggaggagt tccaaaacgt ttctaaaata gatggaatcg caaccattgg
    44941 tcatatcacc gctgaatcat cgggggcaaa cctgatttca cccgcaggcg atgaaataag
    45001 gctgactgct cagggttgga acgcactggc taaggagtag ccttcaaaat aaaaaacccc
    45061 ggaaatccgg ggttactcat ctaacagggt tgtctaaaac cccaacaggt ggcgcaggaa
    45121 attcaccagc ttcggcaatt actcgcttac cacactctta tactgctcgg cggttagcag
    45181 gttgttcacc tcagcagggt ttgtcatttt gattttgaca atccagccct ccccgtaagg
    45241 atccttgttg acaacgtcag gttggtctgt aattttaggg ttcacctcca gcacctcacc
    45301 actaacaggc ataaacatat ccgatacggt tttaacagcc tcaacagtcc caaaaacctc
    45361 ctcatgcccg agggtttcgc caacggtttc aacctcaatg aatacgatat cgccaagttg
    45421 cgactgggcg aagtcggtta tcccaacagt tgcctcgccg ttaccttcca cttttagcca
    45481 ttcgtggtcc ttggtgtact taagatttga tggaatgttc attgctataa ttttttagta
    45541 gttaatattt tagttggtaa aatattttca aaagtaagca tttttgcaat tgaaatcgaa
    45601 agatactgaa aaataaagtt aatgcggagc agtattactg gttcacaccg ggtaattgtt
    45661 ggccttggtg tttattgcct tagcacatca gccaaaaggg gtggcaggga gcaggagcca
    45721 tctacttaac cagctcaaac ctaacgctaa acccgaagct ggtattggtt gtgcggtagg
    45781 ttctggaaac ttttggggcg ttgagggttc ggtcgaaaaa cagccttacc gtaacctgct
    45841 cgttgatgcg gtagtcggcg gaggttttga tggtattaac gagttgtccg gcaataattt
    45901 cggagtaatc ctccaccagc ttgcgcaaaa tggttgtgtt ttcgcgtatg gagagatcaa
    45961 cagtaatccg caggtcgctc ttaacggcgc tcttaccgcc atcctccgac tgaaacacaa
    46021 ggggcaaatt ctcgaacctg tagcctgccc cgataatata ctcgtttccg taaatttcgg
    46081 taagctggtt gttggcaaaa ctcattgcca gcgaacggct ggttttaaac tcaagtcggg
    46141 tggtgaggtt gttctgccag cccatatcca acccaagcaa tggagaaaat tgctcgttta
    46201 tggaaacgtt ggtaatttca cgttttggca caaaatcgcc atttatgttg cgcacgtagc
    46261 taaaaccatc gtcctcgggc agatagttca ggttggttgt aaaggcgcca atagtatagg
    46321 ccgatcggta gccatgagat agtgtaaagg ttttaaaaag attcttaaat ggtcgaattt
    46381 tagcaagtcc atcgtaggta atctgccagt tgggcattgg aataaccgga aaatctctaa
    46441 gggtaacctt ctgcggcgag gtattggtat aggcagccag gaatgaagcc ctgagcacat
    46501 cctgcgatag aggaccgtag cccaccggga atcccaaaga gtctggtgga gccatcgggt
    46561 cgtaaccctg accgggatta gccactcgtt gatcgcgaag cctctcggca attaccagcc
    46621 ggttgctctt catcctctca aaagccctgg attttgcatc gcggttcttt ccctcaaagg
    46681 tagttccaag cgttagtacg ctgatgctga aattaccggt tgtcagcggg ctaagcgagc
    46741 tatacgaccc tagtgcattt gcccggtagg tttcgttatg gttgcgcgag taggttcggt
    46801 tggccgttag ctcaattcga aaaccgggca caggctcaat gctgctgcgg aagttcaaat
    46861 tttcgttgtg ggtaaacacc accggcgtat taaaggtggt atcgtgtgtg agccagccgt
    46921 tggtagctgc tttccatgca atttccggat cctgccaacc ggcaatgaac tcccatccgg
    46981 gagccgtaaa gccgttgtag ctgtcaattc ctaagtaccg tgtaccgggc ttgtaaccag
    47041 gcagaagcgt tccgttggtt tcggaatagg aaagcgaaag gcttttaacg ctcatcatca
    47101 agcgaagtgt gccctcccac gcaaacttca gcggattggg cctatcgggc accttgcctt
    47161 caaccaccac cctgcagttg ctgtaatcgg catctgccct gatggcaaca cgtgtatcac
    47221 ttttgacctc cgatttaacc tttacctcgt tgcccttctc gtcgtacacc cttacagtta
    47281 ccttatcggt tttgagctta tggttgatat tttttgcgta cttcgctcta atgttcaccc
    47341 cgccttcctc gtacttaacg gttttggttt taggtgctgc ccctcgcccc ctactatcaa
    47401 actcctggtt aattcgctta aggtagccaa ccttgttgaa aagagtatta atgttgattt
    47461 gaccgttaag ctggatggtg ttggagtttt gaacggtatt acctgggtcg aatcgcgatg
    47521 tatcggccat aatggggcct gcctgccacc ggtaaattcc agtgtagcgt gctgaggcat
    47581 tagtccagtc aagaaatggg atcttactta gtggaagcga gtaggaaagg ttaaactggt
    47641 ggttatacat ggtattacgg ccaaaattct taatgttctg ccacaccgag tcgcgccagt
    47701 gctcgtatcc tttagggtcg cggtttcgat caaccatgcc ctcgggttca tcaatacggg
    47761 ctacgttgct ggcagtaaaa tcaaatttca acgattgggt aatgtcccag ttcagagcat
    47821 agttgcgatt ccacagaaag tccttggagt aggttggaag caacacggcg ctggggttgc
    47881 ttacgttgcg aaactgctgt tcaaagtaac ttcgatccaa atcggttcgg aaggagagct
    47941 gagctggggt aaggttaaag ttgaagtcct taatcaacct gaaatatttt gagttcaacc
    48001 acttcacgtt tttaaagggc acaatgtact ttggctgctt gttgaagttg taggttaggg
    48061 cgcctctaat atcccgttga atcttatgat cggtgcggat attccttgag aacagctcgc
    48121 tgtaggagta gctcacggag aagttggaaa tgttccaaaa atgaggtttt tcagtagtct
    48181 tgtcaatttt aacatttgta aagttgatgc ttcgatgtcg ggagtaatcc tgcacaatcc
    48241 gctttacgga atctctttcc gaagggttgt aggaattcag ggagctgcta agggttacat
    48301 cggggtcaag ggggttatac tggggattgg atatggactc gctgaacccc aggaacatgg
    48361 gtattcttac gtttaacttt tcggggaaaa acttacccag ctccagggtg gagttgatat
    48421 cgtactggta aatagtttcc ttgctgcgct cgttaacctt tttctcgata gagccaaatc
    48481 cgggagtaac ggttgaaccg gccacattta tcatccctaa atcggccagc ttggccgaaa
    48541 ctcgggcatt agcggcccat cccccctgat cgttaaagtt ggtaagtcga aactcgttga
    48601 accaggccac cactgattta ggcattccgt catcggttgg accctttccg ttagccgggt
    48661 ttctaatacc tatcataatc accttaacgt tgctgaggtt gggatttccc accaccttca
    48721 tctgggcatc accatcatca accgtgtaga gggttcgttg ggttaccatt gagcctgggg
    48781 ttcgcatggc atcgttacgg gccaccttaa cctccaccag cttttccaga tcaatattga
    48841 ttagattctc acgaggccaa accatttccc tatccgactc ccggctgtta aagtagtagc
    48901 cgtagggtgt cagggacaag ggaatttcgt actcgtagta gttgtcggta taatcggttc
    48961 ctattcggat aaatgccgaa acatcgccat ttgacaacgg ataaccctca accatctcgg
    49021 cgtgcacgtc cattttaagt tttttgtact gccggatatc tatattgaca tttttgaaag
    49081 ccgctctggc atcgccatcg gccaggtcaa tcaccctgta ctccatggcc tgctcgttga
    49141 gctcggtgag ctgaggctgg ctggggtcaa taaccctgtc aacgcccggg ggcagcacgt
    49201 agttaaccgg cgtgcgtttg tcgttctcct caatgttgac cgaagagatg gcaaaccccg
    49261 tggtgggtaa atcgcccgaa ggaataccct cctgcccctg aataagcgag tagttatact
    49321 tacgccactc gcttttcacg agttcaaggg tggcaaatct caggatcatg gtgtcctgaa
    49381 agccccgcag gaacattctc atgaagcgga tggacttaaa atcgtcaatt gggccgacca
    49441 cccgctcata ctccgtaatg ggaattttaa actggtacca cttcaccgga acgtttctaa
    49501 gcttatcctt ctctccggat atcacgttgg taatgtagtt tctgcccacc tccatgtcgg
    49561 caggtcgaag ggatatcctg tactggtagt atgcctcgct ttcgctcatg gtgatgtcgc
    49621 cctcgctaag gttttcaaca tcgggcgtgg agtaggcctg ctgggatgtg gttccggttg
    49681 caacaggtga gttgccctcg gtgttgttga aatacttata cctgtcgagg atggtagctt
    49741 tgctctggtc gtactggttg ttcaggaaat ggataaaatt atcgttggac gggtcggcat
    49801 cggattttgc cataacctcc tcgttggata ccctgaagcg cagctgggta aggtagttct
    49861 ggaaaaatga ttgttcatcg ggaatgccgt cgccgtcgag gtcttcgcct gaaaggccat
    49921 caagccctat atcctggtac ttacggttgg cggggtcatt gtcgaatcct acaggaaggt
    49981 actgcttgcg gggcactcta ccccaaacag tagtgtcaac cttctccatg gcctgggtgg
    50041 tgggcatgcc attctcaaac gacttgcgac cgtcgcgaag tatatcctcc gaaacattgc
    50101 caaggttgat gtaaaaatcg cctccgctca tggtggagtc gtaaacaaag ggatccatca
    50161 gccaaaactc gatgtactct atgttggctg tttcaaagtc ggtagttcca atgctccgca
    50221 taacaccacc ccagcggttc tgtgggttaa gcagtcgtcc atcagggtca acattaatat
    50281 catcgtagtt gtatggaccg cgctcatcag ggtaaaagga caggttcagc accgatatgt
    50341 tggtgggctg cccctgcggt gtgttccggt tggggaacag ctcacgttcg aaaatttccc
    50401 gaacaaaatg cgatccctgc agctccttgt tgttgcggat gtaggatggg gtaagggatg
    50461 tattccgcaa aaaaagcggg tcgatgaagt accatgccag catcgctcta ttcatgccgt
    50521 tgcgcaggtc gttgctataa gaggcctcag ggaataaggc aggttggccc tggggtgtgg
    50581 aggcaagctt ccactcggtc cagtagtgct ggtcgagctt agtgctgctc ccctcaaaat
    50641 cgtcaaggta aactgttccg gctttgccta tagcccttga gtgcccggga ataagatgcg
    50701 caaactcggc gtcgaaggtt acagttgact tctccttggt ctgaataaat ggtaaaaaat
    50761 caaccatgcg ggtaagcagc cgagagtcgg tttggtagga ggtattcaac ccccagatgg
    50821 tatttgagat agcctcgttt ccaaaactca ccttttgggt aagcgggcgc tcggtaaggt
    50881 tcataatggt ggcgcccacg ttgaagttgt cggagaacct atagtcaaag tggctgccaa
    50941 ttagtgtttt agtttgaagg ctgaacaagg cgttgctttc caccgaaatt ctaatgggag
    51001 tacccgattc aagcagcgcc tggtctaaaa tgcgcaccga gccaagattg tagtcaacaa
    51061 tatactgaac atcttcctga agcttagcac ccccggccgt aaccaccact gaacccttag
    51121 gaatgtttgg agcgttaagg tatatctccg atccacctgc cgactggtag gagccgctaa
    51181 gaataaactt gtttttttcg gccatctgcc tcgccctggt aagggtggag tcgtatagtt
    51241 cctgaaaaac atacttatcg gcaatcgccg gattggtaaa tttagccttc aaatcgcggc
    51301 caaatggttc aagtaccgga aaaattatct ttccccctgc cgggttaatg gttaccccct
    51361 caataaaatc gaacacacca tcgggtatag catcaagctg gttgttgagg ttatcgaggt
    51421 ttaacacgcg aataagcggg atgttggcaa tagcatcctc gccaatgtag cttaaaggtg
    51481 ttcctgtttt atcgttctcg tagagtacat cgagcctaaa ctcattcttg ctaacctgga
    51541 aagcgttcat ggagtatata ttcttcatca tcagcttcca tgttggcagg gaagggctta
    51601 ggttggtacc cttaagcagt ttcacaacaa gtgcttgggg tgcagcaatc ccatcggttg
    51661 aaagctcacc aaccctgtag gtttttccat cctgggtgta ctcaaaggca acagccagca
    51721 tttcgtcgtt atatagcgga gagttcagcg aaatgtagcc taaaacaggg ttaaaggtaa
    51781 actcgttgga agaaagccgg cgtgcatttt caatcttttc gtaatcacga cccccgctaa
    51841 acccacttcc ctcaaggggc aaaagggtgg agttcacctc ggctatatcg cgaatgccgg
    51901 agtatgttgt ggtcatcaac tcgtagaggg tgttccggct gttatcggcc gggccgggaa
    51961 cggtttggtg aaaaatagag ctggcaaaaa tattcggatc ggcctcaccc aggtcggcaa
    52021 atgccaccag atttcgggag ttctcaaagt tggcctgctt gttggttacc caaacttcaa
    52081 ttttggtgat gttgatgccc gagttaatca ccggtaggtt ggccagcgcc ctgttatagt
    52141 tctcacgaaa gtagtgcgac aggaaaaaat gccggttggc gtcgtactca tcggcccgaa
    52201 tctggaagtc acgcacctga gcgccaccct taacctcaac tacctgggtt tgaccattct
    52261 gcttcgacac cacactggta acgtaaagct tcccgaactt catctcggtt ttaaagccaa
    52321 aaaggctttg gcttccggta attagggtgc ccggcagggg aagtgatacg tttccagcct
    52381 caatccgctg gataatttcg tcctcaaaac cgttgtactc caccttcacg ttattctcga
    52441 aatcgaaagt tgcctcggtg ttgtagttca cctccatctt caatttctcg ccaatttttc
    52501 cggccacgtt catctggatc ttcgactgaa aatcgaaggt ggtatttttt cgctggtcaa
    52561 ccggtagtgc agggttttcg gttttagagg aggatatgcc aaaaaccagc tcagcattac
    52621 cctgggggat aatctcaata acattgctcc caaatatcct atcaaacgac tcgccaccca
    52681 cctgaatgcg tggtagaatg ccatcgccct ttccaactgt ttcgccggtt tgcttttgcc
    52741 gccagtactc ccgtatggac tgctccaccc ggtagttctg gtactcctcg tcgctcatca
    52801 cacgaggcaa acctacgtta tacccgccaa ctcgctggta aacagtatat ttctttgtgg
    52861 tggggtcgta ctccaccacc tcggtaacat tcgatggctg gtttagaaac aggggtgaac
    52921 gcaccgttgc cggtgttaaa aaattttcag attttattgg gtagggtagc ctggtggtat
    52981 cctgcaggtt aaccgtttgc tcatacactt gcccaccgat ggatacacgc ggggtgaaca
    53041 gcagaaagca tgaaaataca aacaacagca aggaacctat tttaacgcgt gccaaagtca
    53101 atgcctctcc agtgataaag ttctacaagc gctttaatgc cgatttaatc agcccctcga
    53161 cagtcaactc cccgccttcc ctgataattt gatcgagcac cttttcggtg gcagacttgg
    53221 aaaaaccaag cataaccaat gcagataacg cttcatccct cgaagtattg tgtgctgcag
    53281 gcataatttg tttggaagca aagtcatcgc ccaccacttt atcctttagg tccaccacaa
    53341 tgcgctgggc tgttttcagg ccaatgccct taacctgctt tatcaggtta atattttcgg
    53401 taagaatggc cgcctgaagc tcatcggcgt taagcgacga aagaattaaa cgagctgtgc
    53461 tcgcgcctac acctgaaacg ctgataagca gcctgaaaat ttcccgctcc tttgcttcga
    53521 aaaagccgta aagcaaatgg gcatcctccc ttatcacctg atgaaggtag agctttacct
    53581 cattcatacc gttgatggcc gagtatgtct gcagcgaaat gtttacatgg tatcctaacc
    53641 ctccaacttc gaccacagcg tgggtgggcg taagttcggc cagcttgcct acaatatagt
    53701 cgtacatttc ctttacgttg gtttgaatta agtatgggct attaggctga tttgtttggc
    53761 gttatgagcc actactttca ggtagagctg atataaggat ttggtggcaa gcacacctca
    53821 acccattgct aaactaacca ttttccgagc tagaaacgct caaagggttg gtagtgagct
    53881 ggtcatgcag ttttcggttg ttgaatatgt ctatggccag gtatagggca tttctaaacg
    53941 agttggggtt agccaagttt tgcccggcca aatcgtaggc tgttccatga tcgggtgaag
    54001 ttcggataac cggtaggccg gccgtgtagt ttaccccgct gtcgaagcaa attgccttga
    54061 atggggtaag tccctggtcg tggtacatgg ccagaatagc gtcaaaccga gtaaaaccat
    54121 cggaaccaaa caggccatcg gctgggtatg ggcctaacgc tgcaattccc tcatcagcag
    54181 cctgctttat ggcaggtata attgccgatt cctcctcacc tcccagcaat ccggcatcac
    54241 ctgcatgggg attaagcccc aacacagcaa tacgtggcct gcggatggca aaatccctta
    54301 ccagcgtttg gtttagcaaa cgaatttttc ccagtaccgt atcacgggta attaacgacg
    54361 agactttcga aagaggcacg tggccggtta ccacgcctac cctcattaaa tcgccaacca
    54421 taagcataag gaattccttg gcattaaacg agcttgccag atactcggtg tgtcccggga
    54481 agctgaattt tgcactttga atattggcct tgttaatggg tgcggtaatc agcacatcaa
    54541 ttttaccggc cttcaaatcg ggcataacag cctccaatgc aagcagcgag cccttacctc
    54601 cttcctcggt tgattttcca agctcaaccc tcacctcgtc atccatgcag ttaatgatgt
    54661 ttaccctttt cggattggcc tcagccgcat cacgaatgtg gttaaagctg aaattttcaa
    54721 cattcaacgc tttccggtgg taggctgcaa ccttaggcga tccgtaaaca accggagtgc
    54781 agagttcaac catccggggg tccataaagg ttttaattat cacctcgtag cctataccgt
    54841 taatgtcgcc ctgggtaatt cctaccctta ttttctcatc gctcatgtag tttctttcag
    54901 ttagtagggt taaaacttac aaacgtactt gataattttc aattaaccac agactaaaga
    54961 aaaactttta caggaacacg ggtaatgctg attaagctta cactcaacag atttgttcct
    55021 gctattcagt agctacaaaa ttatttgtgc gccagctctt caaaatttta aactatacag
    55081 aagaaagctc agaacctgtc cgccttgcgg cggaacttgt ccggctcctg acggattcac
    55141 ccttcgactg gtgttacagt gagcctgccg aactgctcac tataacattt gtcgaactgc
    55201 tcagggttca gacttgtcca ccttacagtg
//
LOCUS       DAOPHX010000003        44170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_764_length_44170_cov_6.307560, whole genome shotgun sequence.
ACCESSION   DAOPHX010000003 DAOPHX010000000
VERSION     DAOPHX010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 44170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44170
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_764_length_44170_cov_6.307560"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..156
                     /locus_tag="PK502_00505"
     CDS             7..156
                     /locus_tag="PK502_00505"
                     /inference="COORDINATES: protein motif:HMM:NF025274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease C-terminal domain-containing
                     protein"
                     /protein_id="HPG99328.1"
                     /translation="MPILGAGICLLQMAALPLATWLRLIIWFAIGLLIYLFYGRNKSK
                     LNSGL"
     gene            complement(667..2721)
                     /gene="htpG"
                     /locus_tag="PK502_00510"
     CDS             complement(667..2721)
                     /gene="htpG"
                     /locus_tag="PK502_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPG99329.1"
                     /translation="MQTGKIGVTTENIFPIIKKFLYSEHEIFLRELISNAVDATQKLK
                     TLASVGDYKGEVGDTTIRVKLDKKAGTLTISDSGVGMTADEIDKYINQIAFSGAEEFM
                     EKYKNQANGIIGHFGLGFYSSFMVSDKVEIVTKSWRDDAGAVKWSCDGSPEFAIEEAT
                     RSSRGTDVILHISDESKEFLEESRISELLLKYCRFLPIPIAFGTIKEWKDGKEVDTGK
                     EKIINETNPLWVRKPVDLTEEDYMNFYRNLYPTQEDPLFYIHLNVDYPFHLTGILYFP
                     RIKTNFDIQKNRIQLYSNQVYVTDSVEGIVPEFLTLLHGVIDSPDIPLNVSRSYLQSD
                     SNVKKISSHITKKVADRLQEIFKNDREQFEKKWDDLKLFITFGMITDEKFYERAEKFA
                     LLKNVDGKYFTLEEYEKLVKDNQTDRNKNVIYLYASDREAQHSYIEEAKNRGYDVLLI
                     DGQLDPHFINTLESKLKDTRFARVDSDVVDKLISKEETREVSLSAEEQDDLRAVFEGA
                     LPEGDHYWVSFEELGETANPVIITHSEFMRRMKDMAAMSPTASFYDKMPDSHNLVVNT
                     AHPLVKAIVEAKNKQVGGKLKELNAGIEPLKVSVDELHKAQMEKKEEEIPQAQKEELA
                     EREKQLEELKEERRNLLKNFGREYKLVKELTDLAMLANNMLKGEDLSRFVKRSLELIK
                     TE"
     gene            complement(2915..3439)
                     /locus_tag="PK502_00515"
     CDS             complement(2915..3439)
                     /locus_tag="PK502_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPG99330.1"
                     /translation="MPIIGTKYRLFIFVVESNPDCVARLMQGLEVPEQSLIKAFSSGE
                     SFIDFFPKSKIPKRKLILIFLGYTFSDEANQNMMNGIEILETVKMLNPAVHVVMLTHN
                     GEQEYGGHAKSMGANSCIMKDESMVIRANSLIRLLLSEKKLGNRRRQLKVAAVSWLVA
                     LVCFLLSVGVCCFS"
     gene            complement(3523..4176)
                     /gene="pyrE"
                     /locus_tag="PK502_00520"
     CDS             complement(3523..4176)
                     /gene="pyrE"
                     /locus_tag="PK502_00520"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPG99331.1"
                     /translation="MTYSEAINAESIAANLLKIKAIRLSPTAPFTWASGLKSPIYCDN
                     RITLSYPDVRNRICNAFVELVRRLYPDVELVAGVATGAIAHGMLVADRLNLPFVYVRS
                     SPKSHGLANRIEGEYRREQKVVVIEDLISTGQSSIAAVEALKEAGCNVLGLLAIFSYQ
                     LEQADENFTSAGVELTTLTNYSELIGVAVSTGVVNASQLQLLMSWHENPESWGNITL"
     gene            4157..4780
                     /locus_tag="PK502_00525"
     CDS             4157..4780
                     /locus_tag="PK502_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPG99332.1"
                     /translation="MASEYVIFFNDRRFTITSDLAGYFVGNEKGYFLGHASSYDIPKL
                     VKIFQSHPAICNVFVGNGSVDKLFSWFTSQFKFLAAAGGLVRNNAGQYLMIYRRGKWD
                     LPKGKVEPSETPVEAGLREVEEETGISGLTVVRPLTCTYHTYEEKGNTMLKQTHWFLM
                     QVRGTLSLKPQVEEDIEIAEWVDADGVENRLKDSYPSIRMVFASLRS"
     gene            complement(4777..6186)
                     /locus_tag="PK502_00530"
     CDS             complement(4777..6186)
                     /locus_tag="PK502_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPG99333.1"
                     /translation="MRNLVLSLLVFITLGVTGVLAQQALVYGTAADYAGTELVFYTYS
                     DYITLTEVEIGRCRVKANGNFEGSVKVLETTYVFSHLGIYRIYFFAEPGKRYQLVLPA
                     REEKTEAQRLSPFFRETDLQVGVANISQSDINFLVNAFDIRFNAHFDQIVADTYRGKS
                     QNVDSLINVMEEKFKNEPNSYFKAYREYRYGLLRQVTLSRKSSGISNELFLNKPILYS
                     NPSYMELFNLVYDKYFMFFSRTSTGNAIFSTISQEKSLTQLKKLLATNTVLSNDTLME
                     LVILKSLYDEFFSSNFSRSALLTLLDSVYRTTQIPEHAIIAENIRSRITKLLPGFVPS
                     PFKLKDVNGHEVTLDQLEGKYVYLNFCTTTSYTCLQDFSLLEKIYRKYRKKLEIVTIS
                     ADMEKKDLESFLKTTGYSWTFLHFGEKPDVIKDFDVRAYPTYFLIGPDRKIILSPAPS
                     PREGFEQRFFELLRSRGEI"
     gene            complement(6287..6760)
                     /gene="coaD"
                     /locus_tag="PK502_00535"
     CDS             complement(6287..6760)
                     /gene="coaD"
                     /locus_tag="PK502_00535"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPG99334.1"
                     /translation="MSKVALFPGSFDPITVGHESLVLRALSIFDRIVVAIGNNSSKKS
                     YFDIDLRKAMIEKVFANEPRVEIICYDGLTVDICNKLNINHILRGLRTAADFEFERAI
                     AQVNRAMYPHIDTIFLLTAPEHTFVNSSVIRDILQHHGDATKFIPASINIYDYMG"
     gene            complement(6992..7073)
                     /locus_tag="PK502_00540"
     tRNA            complement(6992..7073)
                     /locus_tag="PK502_00540"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7037..7039),aa:Leu,seq:tag)
     gene            complement(7140..7865)
                     /gene="lptB"
                     /locus_tag="PK502_00545"
     CDS             complement(7140..7865)
                     /gene="lptB"
                     /locus_tag="PK502_00545"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPG99335.1"
                     /translation="MRLFTENLVKRYKTRTVVKGVSVEVSQGEIVGLLGPNGAGKTTT
                     FYMIVGLVKPNEGKIFVDDVEITGEPVYKRAQRGISYLAQEASVFRHLSVEDNLKAVL
                     EMTKFSKEYQMEKVESLLTEFGLQKIRKSKGIQLSGGERRRTEIARALALSPKFILLD
                     EPFAGVDPIAVEDIQNIVAHLKDKNIGILITDHNVHETLSITDRAYLLYDGEILKSGT
                     SEELADDEQVRKLYLGQNFELRK"
     gene            complement(7914..10364)
                     /locus_tag="PK502_00550"
     CDS             complement(7914..10364)
                     /locus_tag="PK502_00550"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HPG99336.1"
                     /translation="MRRLLFFLALQLAFVSSIFAQGQITKIRGRVIDAQTREPIAYVN
                     IAFRGLPIGTITSEKGEFFLETRTTGDSLTFSYVGYQSKTVAVKKGYFQEINVELQPE
                     AIELASIVVKPGEYQANRIVKNIIRNKDKNNPANLSYFCHSYNKIQVDLNNVNLEASQ
                     RKFLNQFKFIWDYVDTNAITGKPYLPIFITESLSDYYYRSSPKMEKEIIKATKISGVN
                     NESVAQFTGMIYQNVNIYDNYINIFDQGLVSPIANAGLIYYKYILVDSAFVGNRWCYQ
                     ISFKPRRKMEPTFTGDFWVNDTTWAVVKAQFRLSDMVNLNFVNDMVTTSEYVPINDTL
                     WFPKQLTMFVDFNLTDKEAGFFGHKTVTYSDVTLNPDFPSEITEQPTSLKVMEGALNQ
                     DKEYWNKTRPFELTPKEANIYAMVDSVQQVPLYKTFVDIINMIVNYYYVVGYVEIGPY
                     YQTYSFNEIEGNRFKVSGRTSNEFSTKVMLSGFLAYGDKDNRFKWGVGSMYMIGKNPR
                     QVLSLDYKSDIEQMGQSPNALTEDNILTSFLRRNPNYKLTMVKDFSVSYEKEWFTGLS
                     NTIYFSNRIMYPTEYIPFLPVGGGLQLKNITTSAVTLNTRWVKDETFVQGEFTRVSMG
                     SKWPEVNFDITKGIKGVLGSDYSYWKLHLNYYHKFNVNPFGYARVIADAGKTFGEVPY
                     PLLQLHEGNETYAFDRYAFNMMNYYEFASDQYASLYLEHHFQGFFLNRFPLLRRLKLR
                     EVATAKGLIGSISNSNMNVLQFPEGLGSVSKPYVEVGVGVENILKVIRIDAIWRLTHL
                     DNPDISPFGIRVGLQIIF"
     gene            complement(10376..11176)
                     /gene="tatC"
                     /locus_tag="PK502_00555"
     CDS             complement(10376..11176)
                     /gene="tatC"
                     /locus_tag="PK502_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPG99337.1"
                     /translation="MAGKASKHNEMTFWQHLGELRNIFIRCMLAVVVLSIVAFCLKDI
                     IFQQVVLRPRMPEFVTNRLLCRLAEQWNSPVLCINSTILPLINIELAGQFTVHLRVAF
                     WSGVIMAFPYIMYELAKFFRPALKQGESAMLRGVALMFTLCFAIGAAFGYFIIAPISI
                     HFLGTYSVSAQVVNQITLGSYLSTVLGLIFAGGAAFEMPVVVLYLSRLGVVKPQFLSR
                     YRKVAIVVILVISAVITPPDVFSMTIVAIPLLLLYEVSVLLARMEFKD"
     gene            complement(11190..12185)
                     /locus_tag="PK502_00560"
     CDS             complement(11190..12185)
                     /locus_tag="PK502_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HPG99338.1"
                     /translation="MTKVANERKKMNNDAIRKLAVETIRTEADAIEKLAGFIDDDFVM
                     AVNLIFNGKGRVIVTGIGKSAIVAEKIVATFNSTGTPAIFMHAADAIHGDLGIIQPDD
                     VVVCLSKSGSTPEIKVLVPLIRNLGNKIIAVVSNTDSYLARSSDFVLKATVDREACPN
                     NLAPTSSTTAQMVIGDALAVCLLKLRGFTPADFARFHPGGALGKKLYMRVSDIFTSTD
                     VPKVRPDDRIRSVILEISSKRLGATAVVDSNDRVVGIITDGDLRRMLQKNQSIEGLAA
                     ADIMTVNPKTIDREELAITAFNLMEQNKITQLLVTHEDRYEGVIHIHDILREGIV"
     gene            12338..14536
                     /gene="recQ"
                     /locus_tag="PK502_00565"
     CDS             12338..14536
                     /gene="recQ"
                     /locus_tag="PK502_00565"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HPG99339.1"
                     /translation="MNVNTSELSLKEYLNKYFGFNTFKGNQEAIIHNVLTGNDTFVLM
                     PTGGGKSLCYQLPALILDGTAIIISPLIALMKNQVDSMRAFVLEEGIAHFMNSSLNKT
                     QLQKVRQDVTNGVTKMLYVAPESLTKEENIQFLKQIKISFYAIDEAHCISEWGHDFRP
                     EYRRIRPIINEIGTAPIIALTATATPKVQHDIQKNLGMLNASVFKSSFNRPNLYYEVR
                     PKVNAVKEIIKYIKSNQGKSGIIYCLSRKKVEEMAEILKINGIKALPYHAGMDAATRS
                     ENQDKFLMEEVDVIVATIAFGMGIDKPDVRFVIHYDIPKSLEGYYQETGRAGRDGGEG
                     QCITFYSYKDIQKLEKFMQGKPVSEQEIGKQLLLETVSYAESSMCRRKTLLNYFGEEY
                     DEENCNSCDNCLHPKTQFEGKEELQMVLETVLAVNQKFKADHIVNILLGNVTAAVKSY
                     KHNQLDTFGEGAEKDSRFWNSIIRQALVMKFLDKDIENYGLLFITPKGQEFIDKPTDV
                     LFTLDHEYEEADDDESIGMPMDRSGAVDDELLAMLKDLRKKMAKKKNLPPFVIFQDPS
                     LEDMAIQYPITIEELHSITGVGAGKAKRFGQEFVELIKAYVEDKDIIRPQDMVVKSVA
                     TKSLLKVYIIQSIDRKMDLEDIANAKNLEMDELLNEIEAIVNSGTKINLDYYINQVID
                     EDKQEEIFDYFRNEAETESLEEALVALGDEDYTEEEIRLMRIKFLSEMGN"
     gene            14889..15923
                     /locus_tag="PK502_00570"
     CDS             14889..15923
                     /locus_tag="PK502_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPG99340.1"
                     /translation="MKKLLLLSPQLVLTLSLFGQTIITSQNHGFIPNDNNPMLLTSYV
                     EPGPAGKNVVWNFSMLETSENFQGSLKEVYTLKDYPSNQGGNVVLEEFGNRFIFNMTA
                     NSLEQLGFQSSTGSTEITYSKPFVKMRYPFGFNSSFSGDFKGDYLVNKVPVGTISGTY
                     RVEGDGYGKLILPGGRELSGVLRVKEVKLTTQTINNSLINIEDVTYRWYVANHRFPVL
                     SLIKSTYTPANGKPSTQTKAAFNGNVVSQPSDVDKVSDNLLRFTTFPNPYKGKVAITY
                     TLPESCKVNLAVYDITGKLVSVLVNGIEDAGQKTHYFSAAEVGMPAATYIVKLTAGNV
                     EFIGKVLEVR"
     gene            complement(15932..16984)
                     /locus_tag="PK502_00575"
     CDS             complement(15932..16984)
                     /locus_tag="PK502_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99341.1"
                     /translation="MRGKLLMLLVASGVILSVSAYGQNNLPAYLGVSYIPELRWKVYF
                     INHKSIGEPLSLNFDMTSMSSYEGNFGIRRLGVRMGVSAQTENNLIGKFYRWGGYLGF
                     KNMLLRLQSSKMSGDLEWSGALPPGYYANREFSNKYFNIDLLKTAKSGSAFNDVGGYW
                     GVGYTSMGYPVEISTLVTEGGRENQKFGMPAYDTLYSIKSYNICFGFDLLRQLCLTGG
                     LEGFAPGRRAMRFAMYASTQDKVGFGPAKISDYGVSMAEALNPGRTAVTSKFFNVMVH
                     YSLSLGLRYYIHPGPTVVIFALGYDFEGAMNAPFKGAADNAEDLGFDFLNYYLNHGVS
                     FKIYLAWNRDWQQKNQ"
     gene            17310..20930
                     /gene="dnaE"
                     /locus_tag="PK502_00580"
     CDS             17310..20930
                     /gene="dnaE"
                     /locus_tag="PK502_00580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPG99342.1"
                     /translation="MGSFVHLHVHSHYSILDGASSIEMLVSRAKELGMPGIAITDHGN
                     MFGIKEFLNTVKKSNSPVLKEIESIKAEIKGISSGSSVADNSPSLDLLNEQLREAEKQ
                     LFKPIVGCETYLAKNSRFDRTDKEDRHNFHLILLAKNLEGYHNLVKLVSYGYTEGFYY
                     KPRIDKELLRQYSKGLIASSACLGGEIPQAILNNNIAEAERLIMEYRDIFGDDFYLEL
                     MLHKSGEPRIDSEVYENQLKVNKALLELSVKTGVKCITTNDVHFVMSDDAASHDLLIC
                     LNTDKRIDDTNRMRYTFQEYLKSEEEMLSIFSEHPEAVHNTMEILDKVEFYDLSHKPI
                     MPNFPLPDGFTDDNDYLRHLTYEGAKLRWGDTLSDEIGSRIEFELQVIERMGYPSYFL
                     IVWDFIRAAREMGVSVGPGRGSAAGSVVAYCLKITDIDPIKYNLLFERFLNPDRISMP
                     DIDIDFDEDGREDVLQYVVQKYGEKRVAHIVTFGTMAAKSSIKDVARVLNLDLSTSER
                     LSKLVPERIGITLPAAIKEVPELEKELKSDNELVRATLKHAQVLEGTIRQTGVHACGI
                     IIGRDDLENYIPLCINKEAKLFVTQYEGNHMEDIGLLKMDFLGLKTLSIVKDALQLIM
                     ESTGKQLDIDNIPLDDSTTLELFSRGDTTAIFQFESAGMKKYLKALEPNRFEDLIAMN
                     ALYRPGPMEYIPSFINRKHGREKIEYDIPEMEEFLKDTYGITVYQEQVMLLSQKLAGF
                     TRGEADSLRKAMGKKLRDVMDKMKGKFIHGCINNGHDQAKVEKIWTNWEAFAEYAFNK
                     SHSTCYAFLAYQTAYIKAHYPGQFMAAVLSRNLSDIKKITTFMDECRRMGLQVLGPDV
                     NESNYKFSVMPNGDIRFGLGAIKGMGENAAANIIETRNHEGKFKNIYDFMERVNLQTV
                     NKKNLESLVLAGAFDNISPISRGAYFTPDSKGVTFMETLMRYGAKVQLEKNSKQMTLF
                     GATNGFEVPQPEPPKTDDWPTIEKLNKEKEIIGIYLSAHPLDEFRFEIENLCNTNLAE
                     LKNLEDFNNREVTIAGMVVKAKIDTTKNGKPYARLTIEDYSDTMDIMLFGKDFETYRN
                     FCYTGYMVMIKGKVQPKAFKPEELELHIKSMSMLYDVREKQIKGISITLPTTEINDIL
                     IDDFTRLTHENAKVSLRFKVVDVENRISVDLFSRSHRVSINTELIHFLADNELEYKLI
                     TS"
     gene            21006..21326
                     /gene="trxA"
                     /locus_tag="PK502_00585"
     CDS             21006..21326
                     /gene="trxA"
                     /locus_tag="PK502_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPG99343.1"
                     /translation="MAQEVNDSNFDEVVLNAGKPAVVDFWAEWCGPCRMIAPYIEQIA
                     DEYKDRAVVAKVDVDNNPGIAARFGIRNIPTVLFFKDGQLADKQVGAVPKSNLVSKLE
                     ALLR"
     gene            21589..22164
                     /locus_tag="PK502_00590"
     CDS             21589..22164
                     /locus_tag="PK502_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPG99344.1"
                     /translation="MRISVYCASSSKINQKYFDATRQLASELANRGDIIVYGGGSHGL
                     MGCLADTALMNGGMVVGILPRFMNKVEWGHKNLTELKLVKDMHERKKLLIKDVDAVVA
                     LPGGCGTLEELMEVITLKRLGKFTKPIIILNTDGFYDYLKLLLAKMIDEHFMRPEHAG
                     IWTFVETPQEVIPAIEKAPTWDSDAIKFAAL"
     gene            complement(22244..23341)
                     /gene="ribD"
                     /locus_tag="PK502_00595"
     CDS             complement(22244..23341)
                     /gene="ribD"
                     /locus_tag="PK502_00595"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPG99345.1"
                     /translation="MDNLDVDKLYMSRCLELALNGMGSVAPNPMVGAVVVHNGKIIGE
                     GFHAQYGEAHAEVNAINSVRDKSLLQDSTLYVSLEPCSHYGKTPPCTDRIVEYGIPRV
                     VVATTDPNPRVSGRGLEQLRKHGVEVSLGILEDEALLLNRRFITFHTCKRPYIILKWA
                     QTIDGFIDLVRTAETPVGSVWITNELARSLVHRWRSEEQAVIVGTNTVERDNPRLNIR
                     SWSGRNPIRVIVDRKLRLSRDANVFDGSQPTILFIGNNSSALVRKSEFAGISNLEMVT
                     IDFAKGSEQQILKELSDRDVISLIIEGGATLLNNFIQKNLWDEARVFVGNRFFGEGVK
                     APLFKGDPVSYDEVGDSKLFIYRNQQGRSNR"
     gene            23385..24245
                     /gene="prmC"
                     /locus_tag="PK502_00600"
     CDS             23385..24245
                     /gene="prmC"
                     /locus_tag="PK502_00600"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPG99346.1"
                     /translation="MKSYTQRQLFDEVGLVLNQFFTSGEVNSLRRMLLEHVLKIPYYH
                     VHLTPNAQVSPSLSKNILDMTGQLVRGVPIQYLLGETTFMELNLTVNPSVLIPRPETE
                     ELVRWVIADCHLPKPKILDIGTGSGCIAIALAQNIPDSTLTAVDISPDALKVARHNAK
                     IYSASICFVEADILSGSLIPNAPFDIVVSNPPYVRSSERRFMHKNVLDHEPPLALFVS
                     DDNPLQFYKAIVSKSSKLIKQGGRLFLEINEEFGNQMVELLEANHFGKVTLRKDINER
                     YRMISGTYTG"
     gene            24268..24762
                     /locus_tag="PK502_00605"
     CDS             24268..24762
                     /locus_tag="PK502_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPG99347.1"
                     /translation="MDSREKVSIDKALTRLQNLCARQEKCVWDIMQKLKLWGINNEEA
                     QKIVSMLVDEGFVDDRRYAQMFVREKSQLNKWGSIKIAAALRVKKINESIIKEALLEL
                     QPQFDRATLENIITTKANQVKAKSPSDLRAKLVRFGLSRGFELEHVVKTVDMVMKSNM
                     TDEA"
     gene            24954..26216
                     /locus_tag="PK502_00610"
     CDS             24954..26216
                     /locus_tag="PK502_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HPG99348.1"
                     /translation="MLKIFTRFDSKNHRPVMGAMEIFKFIGPGLLVTVGFIDPGNWAS
                     NLAAGADYGYKLLWMVSLSTIMLIVLQHNVAHLGIATGLCLSEATTKYLKPGVSRFIL
                     GTGMLASVSTSLAEIMGGAIALQMLFGIPIKIGAVLVLAFVLVMLFTNSYRAIEKWII
                     AFVSIIGLSFIYELSLVNIDWPEAIKSSVTPSFPEGSMLVIMSVLGAVVMPHNLFLHS
                     EVIQSRQWNLQDDRVIRRQLDYEFFDTLFSMLVGWAINSSMILLAATTFHKTQTPVSE
                     LSQAQNMLIPLLGSSAADIFAVALLLAGISSTITSGMAAGSIFAGIFNEPYDVKDNHS
                     KLGILLSLVAAVLVIFAIDNPFKALLISQMVLSIQLPFTIFTQVRLTSSEKVMGKYKN
                     SPFTKWTLYGLGAVVTLLNLMLFVSFLG"
     gene            complement(26281..26676)
                     /locus_tag="PK502_00615"
     CDS             complement(26281..26676)
                     /locus_tag="PK502_00615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPG99349.1"
                     /translation="MASIFSKIVNGEIPSYRVAEDEKYFAFLDINPLAEGHTLVIPKQ
                     EVDYLFNLDQETLSGLVLFAQRVAKAIESVIPCKRIGVAVLGLEVPHAHIHLIPINSE
                     SDIDFHKPKLKLNPEQLDAIAQKIATKFE"
     gene            complement(26689..27162)
                     /gene="greA"
                     /locus_tag="PK502_00620"
     CDS             complement(26689..27162)
                     /gene="greA"
                     /locus_tag="PK502_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPG99350.1"
                     /translation="MSKVNYLTEEGLEKLRKELDHLKSVERPAISKMIAEARDKGDLS
                     ENAEYDAAKEAQGMLELRISKLEDLVANARVIDESKIDTERVQILNRVKLKNLKTNAV
                     VEYMLVSESEANLRENKISIATPIAKALIGKKIGDIVEVQVPSGLMKFEILSISL"
     gene            complement(27272..27919)
                     /locus_tag="PK502_00625"
     CDS             complement(27272..27919)
                     /locus_tag="PK502_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99351.1"
                     /translation="MDGGDILFIIIVTILGIISSVSRKKKEAAKRAMKQTEAEEPKNY
                     SEGQPVEIPENNNEDIYTWPQQSVSEFPSNDTMQDFTSNSNPRNEGKSVLSGWDNMEG
                     EVKSVEYEMPSVQVSEEELYAPKKYEELDKIPTSSGSSLLDQPVSPYETTYEGLASSY
                     QPIAFEEQEEESKAPDMIFEYEKVLEQESNQAGILDDFDPAKAVIYSEVINKKYF"
     gene            28087..28518
                     /locus_tag="PK502_00630"
     CDS             28087..28518
                     /locus_tag="PK502_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99352.1"
                     /translation="MRAKIAKLLVIAASFFLPSSCGEDEPEIVPDVPVHIEFYLNEPF
                     YNNITVPMGAVKIPNEGYNNNGIIVFRLNQEEIVAFDATCPQHIAVNASVALDENGSG
                     TATCPHCHTVYYFYNNGYPTKGYKLKSYQVSFRGDLVGIYN"
     gene            28682..30583
                     /locus_tag="PK502_00635"
     CDS             28682..30583
                     /locus_tag="PK502_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HPG99353.1"
                     /translation="MIPPVKHAPRWLVFVIDLCICLVSITLSYYVRFEFRIGSEQVNA
                     LKLVAPLVLFVRALAFISFRTYAGLIRYTSLKDAERIFMVTTSSTIVLFTINFIVYSK
                     SGQFLIPNSILLIDFCGTNILMIGSRLFYKTLYYTIKNVDIEAENIVIVGIEQFAASA
                     KRAIDKDNMAGLKVVAFIDPNCKQEGNSIEDVRIYNTSKIEFLVQKYGVSRILIAQKN
                     LDPVKKNKIIDKCLQLNVKVQVIPDATAWINGELNTSQLKSINIEDLLERPPIVLDKR
                     RIQNYIKNKVVLVTGAAGSIGSEMVRQISKFSPKKIVLFDQAETPLYDLELELKENLH
                     FYDFESVIGSVTNDYRVNKLFDVFKPNVVFHAAAYKHVPMMENNPTEAVFNNVLGTKL
                     IAETAVRYGVEKFVMISTDKAVNPTNVMGASKRIAEIFTQSLSCQVDTNFITTRFGNV
                     LGSNGSVIPRFRKQIEKGGPVTITHPEVTRFFMTIPEACQLVLEAGAMGNGGEIFIFD
                     MGEPVKILDLAKKMIKLSGLTLGKDIQINFTGLRPGEKLYEELLNNTENTIPTYHPKI
                     MIAKVPDLIPENVIPKINRLIETLPEHNNFKVVSLMKDIVPEFISRNSIYEDIDRQKK
                     YIVNDYKTA"
     gene            30564..31016
                     /locus_tag="PK502_00640"
     CDS             30564..31016
                     /locus_tag="PK502_00640"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPG99354.1"
                     /translation="MTIKPHSYFHSTPIQIRVNDIDPLNHVNNSVYQQYFDLGKIAYF
                     NRVLGEHLNLETEGLVLANITIDYLKPITLYDSIEVFTRIYEMGTKSLKMGQEIVNRV
                     SKEVYTTSKSVLVCFSNSSGLSIPIPLLWRERIADFEKEGLIHGVNLQ"
     gene            complement(30990..31520)
                     /gene="rimM"
                     /locus_tag="PK502_00645"
     CDS             complement(30990..31520)
                     /gene="rimM"
                     /locus_tag="PK502_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPG99355.1"
                     /translation="MTDTQLVEVGFIQRTHGVKGEIQISVASSAGVLPEELELVFLDI
                     EGIPVPFFIQGIVAISDEILIVKFDEVDSVEEAAELVGLRVLLPCGCLSNDDGGVPFS
                     SLVGYRLFSTKNEELGIITRYEEFAYNALFAVTAKSGKELLIPAAENLISRIDDAERI
                     VWMDIPQGLLEVNSMD"
     gene            complement(31527..32069)
                     /locus_tag="PK502_00650"
     CDS             complement(31527..32069)
                     /locus_tag="PK502_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPG99356.1"
                     /translation="MRLSRHGRKKNPYYHIVVADSRAPRDGRYIERIGSYNPLTNPAT
                     IELNFDRALDWLQKGAQPTDTCRAILSYKGVLYKNHLLIGVKKGAITMEQAEAKFDTW
                     LKEKEGKIKAKIDLVENSNAKRKNERLEAEAKVREARAQAVAKKRAAEAEKEAKKSSE
                     NSAQDAVEANADTEAGSSEE"
     gene            32308..33663
                     /locus_tag="PK502_00655"
     CDS             32308..33663
                     /locus_tag="PK502_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPG99357.1"
                     /translation="MPFNRDKVEQWSLAYWLLKCHENFFFKLYFRTEIVGIEKLPDNA
                     TLIFAPNHQNTLMDALAILTLKRDFQPIFLARADIFKKPFLSKILTFLKIMPVYRSRD
                     GFENLLLNDRIFQKTLDVLQNRNGLVVLPEGSHEGVKKLRQLKKGIARIAFQAEDAAN
                     GKLGIQIVPVGLEYSNYVAFRSNLLIRLGEPIEVDRYLELYRQNRAQAYNALIAELEQ
                     RMRLEMINIEDENYTAYLTILELFSARYIKERKLPKSQNQKFQVDKKIISLLDRLKNT
                     NASEFEEIIKNANDYGRIISANGLSVKLLPASSKTLRDLPIRLLLLLITMPVALYGAG
                     NNAIPIAVPYLLSKKFKDVQFHSSIKHAGALLLTPLAYLVQTLVVGFVLKNIWLALAY
                     LATLPLGAAAFYLWRREFFRFTSQLSLRRFRKAKAEEFDLVNHLNNQIYRTVTGLVDK
                     E"
     gene            complement(33836..35179)
                     /locus_tag="PK502_00660"
     CDS             complement(33836..35179)
                     /locus_tag="PK502_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HPG99358.1"
                     /translation="MNKRNVVIIGAAGRDFHNFNTFFRNKEEYNVVAFTAAQIPDIDG
                     RKYPAELAGKLYPNGIPIVSEEELPRLIKEKNVHDCVFSYSDVKYQNVMRISSIVNAA
                     GANFMLLGPNETMLKSTKPVIAVVATRTGCGKSQTSRKVIEYLMGLGLKVVAVRHPMP
                     YGDLVAQKVQRFATVEDLKKHKCTVEEMEEYEPHVVRGNVIYAGVDYEAILREAEKDP
                     SGCDVILWDGGNNDFSFYKPDLTITVADPHRPGNEVSYYPGEVNLRMADVVVINKMDS
                     AAPEGIQMVRDNIRRVNPKAIVVDGASPIKVDNPELITGKRCLIVEDGPTLTHGEMKI
                     GAGTIAARKFGASEEVDARPFLVGKLKETYQIYPNIGRILPAMGYGEQQLKDLEGTIN
                     ATDCDSVIIGTPIDLNRIIKIKKPSTRVYYDLVEIGLPDLKGVLEKFVKQHKLDK"
     gene            complement(35572..36669)
                     /locus_tag="PK502_00665"
     CDS             complement(35572..36669)
                     /locus_tag="PK502_00665"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPG99359.1"
                     /translation="MMKRILISGFACAMLLIGIVDAQSQVARIYFKDSNHDYGVIKED
                     DGVAYYEFQFSNTGSTPLIIQRVETSCGCTTPDWPKEPIAPGKNGRIKVGYNPSGRPG
                     AFSKTITVYSNAETPTVILQIKGEVIPHVKTPDEIFNISIGGKLYFEKTHIPFSRLFP
                     GKIYTDTLKFMNKSSEVVKVAANAGGLNFLTVRVEPQSIKPKQVGNIIVTYDPQKRND
                     WGFVMDRFMLSINDSLITNTPISITGSIEEDYSALSASELEKAPRVEFNTTSYDFGQK
                     AEGEPVEYSFTIKNTGKSDLQIRKVKASCGCTTVQPSKNILKPGESTEIKALFRTNGY
                     SGRQSKSITVITNDPKSSTTVLRLSGNVVKK"
     gene            37130..38323
                     /locus_tag="PK502_00670"
     CDS             37130..38323
                     /locus_tag="PK502_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPG99360.1"
                     /translation="MMASLNDKLKELYKTTPVVAGDIYRHIHQNPELSFRESGTSSYV
                     ASFLESLGIGIIKLDKSYSLLATIDGTGEGKTIGLRAELDALPIDEKTGESYASRTAG
                     VMHACGHDFHLASVLGAAIILNNLREFWSGKVVLVFESGEEELPGGARAILESDEFQE
                     VKPDYMVGMHVLPELTTGKVGFCPGRYMASGDEVYLTARGRGGHAALPHTLVDPVVIA
                     STIILSLQTLVSRKAPSNIPSVLSFGRVTANGSTNVIPDEVSISGTFRTMNEEWRAKA
                     HDLIVQMASDIARSTGGECSVEIRKGYPSVYNDPILTQTAVEVASSIFGSENVAPLEP
                     RMTTDDFAYFSQAIPSVFFRIGVGTPGAPTLQLHRPDFRIDEQAFQTSLPMLTGLCLK
                     LLTSS"
     gene            complement(38430..40196)
                     /gene="sppA"
                     /locus_tag="PK502_00675"
     CDS             complement(38430..40196)
                     /gene="sppA"
                     /locus_tag="PK502_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPG99361.1"
                     /translation="MKSFFKYLLATIVGVLLSFLLIFLIMMGIAGSMVSSADKAVEVK
                     DNSILYMDLKNEIVDRASSNPFETLDLATFEPQKNLGLNEILKAIAKAKDDPRIKGIL
                     LEFDVVNAGAATTGEIREALREFKESGKFVVSYSDTYSQKAYYLASVADSIYLNPEGM
                     VEWLGLRSEIMFYKKALEKLDVEPQIIRHGKFKSAVEPFMSERMSPENREQTLTYLSS
                     IWGSWVDEISKSRNIAVAELNRLADNMEVYDGKSCMKSKLVDSLIYKDQLLEKLKAFT
                     GIPENRNLNTVTLEKYFKVPPVQKTAFSKNKIAVIYAQGEIGMGDGSNTAIGSEGLSK
                     AIRQARRDSTIKAIVFRINSPGGSALASEVIWREVELAGQAKPLVVSMGDVAASGGYY
                     IASPATTILASPTTITGSIGVFGLFFNLQKTLDKKLGINVDVVKTNAHSDFFSMFRPM
                     TAEEKAVGQRFVEQTYQTFIGHVSQGRGIPVERVDEIGQGRVWSGANAKEIKLVDDFG
                     GLTAAIKLAAEKANLDIYRLVELPKQKSPFEELLKGFTTEAKAMLVKDDLGKGYKYYN
                     RVMQMVENQGVQARIPYDVEVY"
     gene            complement(40602..41207)
                     /locus_tag="PK502_00680"
     CDS             complement(40602..41207)
                     /locus_tag="PK502_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPG99362.1"
                     /translation="MNEKGIIKFRCSWVNADPLPYEAVADLNRWRCILYNKGLVGVDC
                     NGVGFGNLSKRYLHNQFVITGSGTGQLTELTTMHYTLVTDFSIGRNWVEAKGPVIASS
                     ESLTHAALYQCDKAIKAVFHIHNLELWKRIIGKVPTTLSNAEYGTPQMAMEIKRLYRQ
                     TDLRKTRVLVMAGHQEGIMAFGLDADEAGKRILSLMKEYNP"
     gene            complement(41204..42316)
                     /gene="mtnA"
                     /locus_tag="PK502_00685"
     CDS             complement(41204..42316)
                     /gene="mtnA"
                     /locus_tag="PK502_00685"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPG99363.1"
                     /translation="MKVNGVNYRTIWLEDGSSPCVKIIDQRYLPHRFVVEPIESFDGL
                     LRAISEMHLRGAGLIGVAAAFGIYLAVWEQRNLVNFQEALEKCALKLIKTRPTAVNLR
                     WAVERQLEVLAGVENPDDRVAIARQTAIDIAEDDAGCCRKIGLEGVGLIEDIARNKNG
                     GVVNILTHCNAGWLAFTDYGSALAPVYEAWRRGVRLHVWVDETRPRNQGASLTAWELL
                     NEGITHTVIADNTGGHLMQHGLVDLVLTGADRITTNGDAANKIGTYLKALAAHDNGVP
                     FYIAAPSSTFDWSLSDGLSQIPIEQRHPDEVRYVGGWLDGRLQNVLVCPDESPAANFA
                     FDVTPARLISGIITERGICKPLPAGIKKVFPEKFNY"
     gene            42634..44148
                     /locus_tag="PK502_00690"
     CDS             42634..44148
                     /locus_tag="PK502_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPG99364.1"
                     /translation="MNKRIFILIIIDLMLILVAFYLSVLLKGVSFASYLESYQNALLL
                     FALIWIATSIVFEKYVFTTHSARKISANIIKSNLLAFAVVTFLIYFTRSDRYSRFIVF
                     NTILMVTLLELFIYNLWAGLKRIRVIPDNLLGMIERSVKRRTPPIQTTRAPNPMRVEL
                     IKRTLQAEFGNEVYEYLNTNTRLFDDKTLIVSTTTPFNIINQPDGYFDTLINLKRTND
                     IRRINKFFEAVNQKLPRGGIFVCMAETQEQRKKRILSKFPPVINWIYYFFDYVVKRVF
                     PKFSLTKGIYFFLTRGENRVISRAELLGRLYSCGFYVNNETEIEKLQYVVAIKNRKPF
                     FDEEPTYGPIIKLRRVGKNGKIIRVYKFRTMHPYSEYLQEYIFNLHNLEEGGKFRNDF
                     RVSTLGAVMRRLWIDELPMITNLFRGELKIVGVRPLSEQYFNLYTPELQERRIKYKPG
                     LVPPFYMDNPKTLEEIMASEVKYLDAYDKHPLFTDIRYLFGAAYNIIFKKYRSS"
BASE COUNT        12365 a        10435 c         9699 g        11671 t
ORIGIN      
        1 ccgcttgtgc caattctggg tgcaggtatc tgccttctgc aaatggccgc cttgcccctt
       61 gccacatggc tgaggctgat tatctggttt gccattggtt tgcttatcta cctgttttac
      121 ggcaggaata aaagcaagct gaactcgggg ctgtagcagc aacatgttta tgaatgcaca
      181 ccggataaat atttacagta ctcgtgcagc tgggcctacc tccttaaaat gggaaaaagc
      241 caaatgcagc tccaacactt gttttaatga tcaacgctgt cgctctacca tcaaaaaaac
      301 aggcatcaaa ctcctgccca tcgagtacga gtaaatgata aattattacc atacgatgct
      361 gcaggaaagc aagaaaacaa gggtgtcccg aaccgtagaa aaaatttgat tgtagctgta
      421 actccagggg ttatctactt caaagacaac cccatgccta catgttaggc ttctgctatg
      481 ccataaggca aaacgctaaa tttttccttc aagccaaccc gctcggctgc tcttctgccc
      541 gttcaacatg gacaagcgat gaggggttca ccagcatcaa ggctttttag ccaaaaaggg
      601 ggtcaggcct caaaattcag acctaacccc taggccattg gttgtggtag aaaatgaata
      661 acgtggttat tctgttttta tcaactcgag gctgcgcttc acaaaccgac ttaaatcctc
      721 acctttaagc atgttgtttg ccagcatggc caaatcggtt agctccttaa ccagcttgta
      781 ctcacgtccg aaattcttga gtaaattgcg cctctcctcc ttcagctcct cgagctgctt
      841 ctccctttcg gcaagttcct ccttctgagc ctgaggaatt tcttcctcct tcttttccat
      901 ctgggcttta tgcagctcat caacgctaac ctttagaggc tcaatgccgg cgtttagctc
      961 cttcagctta ccgccaacct gcttattctt agcttcaaca atggctttca ccaacgggtg
     1021 tgcggtgttc accaccaggt tgtggctatc gggcatttta tcgtaaaagc tagcggttgg
     1081 gctcatggca gccatatcct tcattcggcg catgaactcc gaatgagtaa tgataacggg
     1141 gttagcggtt tcgcccagct cttcaaacga aacccagtaa tggtcgcctt cgggaagcgc
     1201 accctcgaaa acagcccgca agtcatcctg ctcttcagca gacaggctaa cctcacgggt
     1261 ttcctccttg gatatcagct tgtcaaccac gtcggagtca accctggcaa aacgggtatc
     1321 cttaagcttg ctctccagcg tgtttatgaa gtgcggatcg agctgcccat ctatcagcag
     1381 aacgtcgtac ccacggttct tagcctcctc aatgtagctg tgctgggctt ccctatcgct
     1441 ggcataaagg taaattacat tcttattccg gtcggtctgg ttgtccttaa ccagcttctc
     1501 gtactcctca agggtgaagt acttcccatc cacattcttg agaagggcaa acttctcggc
     1561 acgttcgtag aacttctcgt cggtaatcat tccgaaggtg atgaacagct tcaggtcgtc
     1621 ccacttcttc tcgaactgct cgcggtcgtt cttaaaaatt tcctgcagcc tgtcggccac
     1681 ctttttggtg atatggctcg atattttctt tacgttggaa tcgctttgca ggtagcttcg
     1741 cgaaacgtta aggggaatat cgggggagtc tatcaccccg tgcagcaggg ttagaaattc
     1801 gggcacaata ccttcaaccg aatcggtaac gtaaacctgg ttggagtaga gctgtatcct
     1861 gtttttttga atgtcgaagt tggtttttat gcgtgggaag tagagaattc cggtgaggtg
     1921 gaaagggtaa tccacgttca ggtggatata aaatagaggg tcctcctgcg ttggatagag
     1981 attgcggtaa aagttcatgt agtcctcctc ggtgaggtca accggcttac gcacccaaag
     2041 tgggttggtt tcgttgatga ttttttcctt gccggtgtca acctccttgc catccttcca
     2101 ctctttgatg gtgccaaagg caatgggaat gggtagaaaa cgacagtact tgagcagcaa
     2161 ctcgctaatg cgcgactcct caagaaattc cttcgactca tcgctgatgt gaagaataac
     2221 atcggttcca cgcgagctgc gggttgcctc ctcaatggca aactcgggtg agccgtcaca
     2281 tgaccattta actgctccgg catcgtcgcg ccacgatttg gttacaattt ccaccttgtc
     2341 gctcaccatg aatgatgagt aaaacccaag gccaaagtgt ccgatgatgc cgttggcctg
     2401 attcttgtac ttctccataa actcctcggc gcccgagaag gcaatctggt tgatgtactt
     2461 atcaatctcg tcggctgtca tgcccacccc gctatcgctg atggtgaggg ttccagcttt
     2521 tttatcgagc ttaaccctga tggttgtatc gcccacctcg cccttgtaat cgcccaccga
     2581 tgccagcgtt ttcaacttct gtgtagcatc aacggcattc gatatgagct ccctcagaaa
     2641 tatctcgtgc tcggaataga gaaacttttt gatgatcggg aaaatattct ccgttgttac
     2701 cccaatttta cccgtttgca ttgcatccac atttaaaggt ttaacaaatt ttttccagcc
     2761 tctacctgca agttgaatgc catacaaaat ttaccaccgg gtagggcata gaaaatgatt
     2821 atcatcatgt tgatttattg ataattacac tttgtcgggt gattcttaat acaactgcca
     2881 agcggacacg gtaggcgcaa ttttggcagg ttggctagga aaagcagcaa acaccaacgg
     2941 agagtaaaaa gcaaaccagc gcaactaacc agctaaccgc tgcaacttta agctgacgtc
     3001 gcctattgcc caatttcttt tcgcttagaa gtaacctgat aaggctgttg gctctgatta
     3061 ccatgctttc gtccttcata atgcagctgt tggcccccat gctttttgca tgaccaccgt
     3121 actcctgctc gccgttgtgg gttagcatta caacatgaac agcagggtta agcattttaa
     3181 cggtttctag aatttcgata ccgttcatca tgttttggtt ggcctcatcg gaaaaggtat
     3241 agcccaaaaa aatcagaatt agctttcttt tggggatttt ggacttgggg aaaaaatcaa
     3301 taaaactctc tccgctcgag aatgccttta ttagggattg ttcaggaact tccaggcctt
     3361 gcatcaacct ggcaacacaa tcggggttgc tctcaacaac aaatatgaag agtctgtatt
     3421 tcgtgccaat gatgggcatg ctagcacccg tttagttgaa aattaggata aatgtaataa
     3481 aatgaacgaa atatatttat gcccgccaaa gagaaattca gcctacaagg ttatatttcc
     3541 ccatgattca gggttttcgt gccacgacat caggagctgc agctggctgg cattcacaac
     3601 gcctgtactg acagccaccc ctataagttc gctatagttc gtaagagtgg taagctcaac
     3661 accggcgctg gtaaaatttt catcagcttg ttcaagctgg tagctgaata tagccaaaag
     3721 ccccagcaca ttacagccag cttcctttag ggcttcaacc gccgcaatgc tgctctgacc
     3781 ggttgaaatg aggtcttcaa ttaccaccac tttttgctca cgacggtatt ccccttcaat
     3841 cctgttggct agcccatgcg atttgggtga cgatctaaca taaacgaacg gcaggttaag
     3901 cctgtcggca acaagcattc cgtgggctat tgcaccggta gccactcccg caaccagctc
     3961 gacgtcggga tacaaccgac gaacaagctc cacaaaggcg ttgcatatcc ggtttcgaac
     4021 atccggatag gaaagggtta ttcggttatc gcagtatatt ggcgatttaa gacccgaagc
     4081 ccaggtaaaa ggagctgtcg gacttaaacg aattgcctta atttttagta aatttgcagc
     4141 gattgattca gcatttatgg cttcagagta cgtcatattt ttcaacgata ggaggtttac
     4201 catcacatcg gatttggctg gttacttcgt tggcaacgaa aaagggtatt ttttgggaca
     4261 tgcctccagc tacgacattc caaagttagt caaaattttt caaagtcacc cggcaatttg
     4321 taatgttttt gttggtaacg gcagcgtgga taagctcttc tcctggttta cctctcagtt
     4381 taagttcctt gcagctgccg gagggttggt gcggaataac gctggacagt atctgatgat
     4441 ataccgacgc ggaaaatggg atctaccaaa ggggaaggtc gaaccttcag aaacccctgt
     4501 tgaggcaggc ttgcgcgaag tggaggagga aacaggaatt tcaggtctaa cggttgtccg
     4561 cccgcttaca tgcacctacc acacctacga ggagaaggga aacactatgc tcaagcaaac
     4621 ccactggttt ttgatgcagg ttaggggcac tctcagcctg aagcctcagg ttgaggaaga
     4681 tattgaaata gctgaatggg ttgatgcaga tggggttgaa aaccgtttga aagactccta
     4741 cccatctatc cgaatggtat ttgcgagttt acgaagctag atttcgcccc tgctccggag
     4801 cagctcaaaa aatcgctgct cgaacccttc ccttggcgaa ggagcgggag aaagtataat
     4861 ttttctatcg gggccaatca ggaagtaggt ggggtaagcc ctcacatcaa aatccttaat
     4921 cacgtcgggt ttttctccaa agtgcaggaa tgtccagctg tacccggtag tcttaagaaa
     4981 actttccaaa tcctttttct ccatatccgc cgaaatggta acaatttcta atttttttct
     5041 atacttccgg tagatttttt ccagcaagga aaaatcctga aggcatgtgt agctggtagt
     5101 ggtgcagaag ttcagataaa cgtattttcc ctcaagctgg tcgagcgtaa cttcatggcc
     5161 gtttacatcc ttcagcttga aaggacttgg cacaaatccg gggagcaact tggtaattcg
     5221 gcttcggata ttttcggcaa ttatggcatg ttcggggatt tgagttgtgc gataaaccga
     5281 gtcgagaagg gttagcagag ccgagcgcga aaagttactg ctaaaaaact catcgtagag
     5341 cgacttgagg ataaccagct ccattagggt gtcgttgctg agcactgtat tggtggcgag
     5401 gagttttttt agctgggtta ggcttttctc ctgcgatatg gtgctgaaaa tggcattccc
     5461 ggtactggta cgtgagaaga acatgaagta cttatcgtaa accaggttga atagctccat
     5521 atacgagggg ttggagtaaa ggattggctt gttaagaaac agctcgttag aaattccaga
     5581 cgatttccgg ctgagggtaa cctgccgaag cagaccatac cggtactcgc ggtaagcctt
     5641 gaagtaggaa tttggctcat tcttaaactt ttcctccatc acgttaataa gcgaatctac
     5701 attctgggac ttaccccggt aggtatccgc tacaatttgg tcaaaatggg cattaaagcg
     5761 aatatcaaag gcattaacca gaaagttaat atcggattgg ctgatgttag caactccaac
     5821 ctgcaagtcg gtttccctaa aaaatggcga taacctttga gcttctgtct tctcctccct
     5881 tgcaggaagc accagctggt aacgcttacc aggttcggca aaaaagtaaa tacggtaaat
     5941 ccctaaatgg ctgaatacgt aggttgtttc caaaacctta acgctcccct caaagttacc
     6001 atttgccttt accctgcatc tgccaatttc aacttctgta agcgtgatgt agtcggagta
     6061 ggtgtaaaaa accagctcag taccggcata atcggccgca gtgccgtaaa ctaaggcttg
     6121 ttgagccaat actcccgtaa ctccaagagt aataaacacc aacagggaaa gcaccaaatt
     6181 tctcattttc agccttgcta gaatgttagg taatattgct ttaaccggtg gagtaaacaa
     6241 atatcctgcc taagcaggta aaccaccctt gggtttttgc agggttttag cccatatagt
     6301 cgtatatgtt gatggaggca ggaataaact tagtggcatc gccgtggtgc tgaagtatat
     6361 ccctgataac tgaggagttt acaaaagtat gctcaggagc ggtaagtagg aaaatagtat
     6421 caatatgggg atacatggcc ctgtttacct gggctatggc ccgttcaaat tcaaaatcag
     6481 ctgcagttct aagcccacga aggatgtggt ttatattcag cttgttgcag atatcaaccg
     6541 ttaaaccgtc atagcaaata atttcaaccc tgggttcgtt tgcaaaaacc ttttcaatca
     6601 tggccttgcg caagtcaata tcgaagtacg attttttgct ggagttgttg ccaatggcaa
     6661 caactatcct gtcgaaaatg gatagtgcgc gcagcacaag cgattcatgt cccactgtaa
     6721 tagggtcgaa cgaacccggg aaaagcgcaa ccttgctcat gtgatggcag atttagctgt
     6781 aagtagtagc aaaactttaa tcgtacaata gtaggtaaaa attttgaatg ccagtaaatc
     6841 gggaaaatga attgcttaca ccttcaccac caatcccccc acaatggcaa tggagttaag
     6901 ggctattatg actacaaata gcggatttga ggggagagga actattaagg ctggtagcaa
     6961 aacaaaaaac ccggaacttt tccgggtcaa gtgcggatag agggactcga acccacacgc
     7021 cttacggcac tagatcctaa gtctagcgcg gctaccaatt acgccatatc cgcataattt
     7081 ttggcaaagt tagctgattt ttttaagcag ccaaccggga gtagccttaa aaggaaaaat
     7141 tatttacgaa gctcgaagtt ctggccaagg tagagctttc gaacttgttc gtcgtcggcc
     7201 agctcctcgg atgttcccga tttaagtatt tccccgtcgt atagcaaata tgctctgtcg
     7261 gtaattgaaa gcgtttcatg aacgttgtga tcggtaataa gaataccgat gttcttatcc
     7321 ttaaggtggg caacaatatt ctgaatatcc tctacggcaa tagggtcaac cccggcaaat
     7381 ggctcgtcga gaagaataaa cttaggactg agcgccagtg cccttgcaat ttcggttctt
     7441 ctccgttcac cgccggaaag ttgaattccc ttactctttc ttattttctg cagcccaaat
     7501 tcagtgagaa gcgactccac cttttccatc tggtactcct tgctgaactt ggtcatctcc
     7561 agaacagctt ttaggttatc ctccacgcta aggtgcctga aaaccgaagc ctcctgggcc
     7621 aggtagctta taccgcgctg ggcgcgcttg taaaccggtt cgccggtgat ttcaacatca
     7681 tcaacaaaaa ttttcccctc gttgggttta accagcccaa caatcatgta gaaggttgtt
     7741 gtttttccgg cgccattggg cccaagcagc ccgacaattt caccctggct cacctcaacg
     7801 cttacgccct taacaacagt tcgggtttta taccgtttta ccaggttttc agtaaataaa
     7861 cgcatagcta gcttgcatta tttaagctcc aaaagtaaac aaattgttgc tactcagaaa
     7921 ataatttgta gccccacccg tattccaaaa ggcgaaatat caggattatc cagatgggta
     7981 aggcgccaaa tggcatcaat cctaataacc ttgagaatat tctccactcc caccccaacc
     8041 tcaacgtagg gttttgatac ggagcccagc ccctctggaa actgaagcac attcatgttg
     8101 gaattgctga tggagccgat gagtcccttg gcggtggcca cctcgcgaag cttaagcctg
     8161 cgcaacaggg gaaatcggtt caggaaaaaa ccctggaagt ggtgctcaag gtacaagctg
     8221 gcatactgat cggaggcaaa ttcgtagtag ttcatcatgt tgaatgcata cctgtcgaaa
     8281 gcataggttt cgttaccctc gtgaagctga agtaacgggt agggtacttc tccaaatgtt
     8341 ttaccggcat cggcaataac ccgggcgtag ccaaaaggat taacgttaaa cttgtggtag
     8401 tagttcaggt gcagcttcca gtagctgtaa tcgctaccca gcacgccttt tatacccttg
     8461 gtaatgtcga agttcacctc aggccattta ctgcccatgc ttacccgggt gaattcaccc
     8521 tgaacaaaag tttcatcttt tacccagcgg gtgttaaggg ttactgccga ggtggtgata
     8581 tttttcagct gcaaacctcc gccaaccggg agaaatggga tgtactccgt ggggtacatt
     8641 atcctgttgc tgaaataaat tgtattggac aatccggtga accactcctt ttcatacgac
     8701 acgctaaaat ccttcaccat ggttagcttg tagttagggt ttcggcgaag aaacgaggtg
     8761 aggatgttgt cctcggtaag ggcatttggg ctttgtccca tttgctcgat gtcgcttttg
     8821 tagtcaaggc ttaacacctg gcgcggattc tttcctatca tgtacatgga accaacaccc
     8881 cacttgaatc ggttgtcctt gtcgccatag gccagaaaac ccgatagcat aactttagtg
     8941 ctaaactcgt tacttgttcg tcccgacacc ttaaagcggt taccctcaat ttcgttgaag
     9001 ctgtatgttt ggtagtaggg gccaatctca acgtacccca caacgtagta gtagttcaca
     9061 atcatgttga ttatgtcaac aaaagttttg tatagaggaa cctgctgcac cgagtccacc
     9121 atggcgtaaa tgttggcctc cttgggcgtc agctcaaaag gtcgcgtctt attccagtac
     9181 tctttatcct gatttagcgc accctccata accttaaggc tggttggctg ctcggtaatc
     9241 tcgcttggga agtcgggatt aagggtaaca tcgctgtagg taactgtttt gtgcccaaaa
     9301 aaaccggcct ccttatcggt aaggttaaaa tcaacaaaca tggttagctg cttcgggaac
     9361 cagagggtat cgttaattgg gacgtactcc gaggtggtaa ccatgtcgtt tacaaagtta
     9421 aggttaacca tatcggaaag gcggaactgg gctttcacca cagcccaggt ggtgtcgttc
     9481 acccaaaaat cgccggtaaa tgtaggctcc attttccgac ggggcttaaa cgaaatctgg
     9541 tagcaccagc gatttcccac aaaggcgcta tcaaccagaa tgtacttgta gtagattaag
     9601 ccggcattgg ctatcgggct aaccaaaccc tgatcgaaaa tattgatgta gttatcgtaa
     9661 atgttcacgt tctggtatat catacccgta aactgcgcaa cgctctcgtt gttaacacct
     9721 gaaattttgg tagccttaat aatttccttc tccatttttg ggcttgaacg gtagtagtag
     9781 tcggaaagcg actcggtgat gaaaatcgga aggtagggtt ttccggttat ggcattggtg
     9841 tccacgtagt cccaaataaa tttaaactga ttcaaaaact tacgttgcga agcttcaagg
     9901 tttacattgt tcaggtcaac ctgaatttta ttgtagctat ggcagaagta gctgaggttg
     9961 gccgggttgt ttttatcctt attcctgata atgtttttca caatacggtt tgcctgatat
    10021 tcgcctggtt ttactacaat gctggcaagc tcaatagcct cgggctgaag ttccacgtta
    10081 atctcctgaa aatacccctt tttaaccgct acggttttcg actggtaccc aacataagaa
    10141 aaggtaagtg agtcgcccgt agttcttgtt tcgaggaaaa actcaccttt ttcggaggta
    10201 atagttccaa tgggaagtcc cctaaaagca atgttcacgt aggctatagg ctccctggtt
    10261 tgagcgtcaa taaccctacc cctgattttg gttatctgcc cctgggcaaa aatggatgaa
    10321 acaaatgcca gctgaagtgc tagaaaaaac aacaatctcc tcataatcat aaaaatcaat
    10381 ccttaaactc cattcgggcc agcaaaacgc tcacctcgta gagaagcaaa agcgggatgg
    10441 caacaatggt catgctgaac acatcgggag gtgttatcac ggccgaaata accaggataa
    10501 ctacaatggc aacttttcta tatcgggata ggaattgggg tttcaccact cctaaacggc
    10561 taaggtacag cacaactacc ggcatctcaa aggctgcgcc acctgcaaaa atgaggccta
    10621 aaacggttga caggtatgaa cccagagtga tttggttgac tacctgcgca ctcaccgagt
    10681 aggttcctaa aaaatgaatg gatatgggtg ctattatgaa gtacccaaaa gcagcgccaa
    10741 ttgcaaagca aagggtaaac attaatgcta cacctctaag catagctgat tccccctgct
    10801 taagcgccgg tctgaaaaat ttggcaagtt catacataat atacggaaaa gccataatta
    10861 ccccgctcca gaaagccacc cttaaatgaa cagtaaactg cccagcaagt tcaatgttga
    10921 taagcggtaa aatagttgag ttgatgcaaa gaaccggaga gttccactgc tcagccaagc
    10981 ggcataacaa ccggttggta acaaactcgg gcattcgagg gcgaagtaca acctgctgga
    11041 aaataatgtc ctttaagcaa aatgccacaa ttgaaagcac aacaactgcc aacatgcagc
    11101 ggataaaaat attcctcaat tcacccaggt gctgccagaa agtcatttca ttatgtttcg
    11161 atgcttttcc agccatcacc ttaaggaaat tagactatac cttctctgag aatatcgtga
    11221 atgtgtatca ccccttcata tcggtcctca tgggtaacga gcagctgggt gattttattc
    11281 tgctccataa ggttgaaagc cgtaattgcc aactcctccc tgtcaattgt ttttgggtta
    11341 acggtcataa tatcggcggc ggcaaggcct tcgatgctct gattcttctg gagcattcgg
    11401 cgtaaatcac cgtcggtgat aatgccaaca accctgtcgt tggagtcaac caccgcggtg
    11461 gcgcctaagc gctttgagga tatttcgagt ataaccgatc gaattcgatc gtcggggcga
    11521 acttttggaa catcggtaga ggtgaaaatg tctgaaacac gcatgtagag tttcttacca
    11581 agggctccac ccggatgaaa gcgagcaaaa tcggccgggg taaagccgcg aagtttgagt
    11641 aggcaaactg ccagggcatc gccgataacc atttgggctg tagtgctgga tgtgggggcc
    11701 agattgttag gacatgcctc ccggtcaact gttgccttaa gcacaaaatc ggatgaacgg
    11761 gccaggtagg aatcggtgtt cgaaacaacc gcaattatct tattgcccaa atttctgatt
    11821 agtgggacca aaacctttat ttcgggagta ctgccgctct ttgagaggca aacaacaaca
    11881 tcatcgggct gaataatccc taaatcgccg tgaatggcat cggcagcgtg catgaatatg
    11941 gctggcgttc ccgttgagtt gaaggtggct acaatttttt cagccacaat cgcgcttttt
    12001 ccaatccccg taacaattac cctacctttt ccgttaaaaa tcaggtttac tgccataaca
    12061 aaatcatcat caataaaccc ggcgagtttt tcaatggcat cggcttcggt tcggattgtt
    12121 tcaacagcta gttttctgat ggcatcatta ttcatttttt tcctttcatt tgctactttt
    12181 gtcatccttt tgttaaatga ctttaaattt gaaccactaa aaaaaatatg gcgtatattt
    12241 ataaaagcaa aagtatctat tttttatcca cgtaacgtaa tgtagggtgg aaagcaacag
    12301 ttaaacagtt taaaattagg agtcggaaac ttaaatcatg aacgttaaca catctgaact
    12361 atcattaaag gagtatctta acaagtattt cgggttcaac acctttaagg gcaaccagga
    12421 ggcaattatt cacaatgttc ttacagggaa cgacacgttt gttcttatgc ccaccggtgg
    12481 aggcaaatcg ctatgctacc agctgcccgc cctaatactt gatggaaccg ccatcatcat
    12541 atcgcccctc atcgccctta tgaagaacca ggttgactca atgcgcgcat ttgttctgga
    12601 ggagggcatt gcccacttta tgaactcctc gcttaataaa acccaacttc aaaaggtaag
    12661 acaggatgtt accaacggtg ttaccaaaat gctgtatgta gcacccgaat cgcttaccaa
    12721 agaggaaaac atacagtttc taaagcaaat caaaatttca ttctacgcaa ttgacgaggc
    12781 gcactgtatt tctgaatggg ggcacgactt ccgtccggag taccgaagaa ttcgccccat
    12841 aattaacgag ataggcacag ctcctataat tgccctgact gctacggcaa cccccaaggt
    12901 tcagcacgac atccagaaaa acctcggcat gctcaacgcc agtgttttca aatcgtcgtt
    12961 caacaggccc aacctgtact acgaggtacg ccccaaggtg aatgcagtta aggaaatcat
    13021 caagtacata aagtccaacc agggcaagtc gggaatcatt tactgcctta gccggaagaa
    13081 ggttgaggag atggccgaaa ttctcaaaat caacggaatt aaggcgttgc cctaccatgc
    13141 ggggatggat gccgccaccc gctcggaaaa tcaggataaa ttcctgatgg aggaggttga
    13201 tgtaattgtg gcaacaatag cctttggaat gggtatcgac aagcccgacg ttaggtttgt
    13261 tatccactac gacattccca agagccttga gggatactac caggaaaccg gtcgggctgg
    13321 ccgggatggg ggcgaaggtc aatgtataac cttttacagc tacaaggaca ttcaaaaact
    13381 tgaaaagttt atgcagggga aaccggtgtc ggaacaggaa attggcaagc agctgctgct
    13441 cgaaaccgtg tcgtacgccg agtcatccat gtgccgtcgg aaaactcttc tgaactactt
    13501 cggggaggag tatgatgagg aaaactgcaa ctcgtgcgac aactgcctac accccaaaac
    13561 acagtttgag gggaaagagg agcttcaaat ggtgctggaa acggtgctgg cagtcaacca
    13621 aaagtttaaa gctgaccaca tagtcaacat cctgcttggc aacgttaccg cagcggttaa
    13681 atcgtacaag cataaccagc tcgatacctt cggcgagggc gccgaaaagg actcccggtt
    13741 ctggaactcc ataatccgtc aggccctggt gatgaagttc ctggacaagg acatcgaaaa
    13801 ctacgggctg ctatttatta cgcctaaggg tcaggaattt atcgacaaac ccaccgacgt
    13861 gctctttacc ctggaccacg agtacgagga agccgatgac gatgaatcta ttggaatgcc
    13921 catggacagg tcgggcgctg ttgatgacga gctgcttgca atgctgaaag acctgcgaaa
    13981 gaaaatggcc aaaaagaaaa accttccccc ctttgtaatc tttcaagacc catcgctgga
    14041 ggatatggcc atccagtacc ccattaccat tgaggagctg catagtatta ccggagttgg
    14101 cgccggaaaa gccaagcgct tcggacagga gtttgttgag ctgattaagg catacgttga
    14161 ggataaggat attatccgac cccaggacat ggtggttaaa tcggttgcca ccaaatcgct
    14221 tctcaaggtg tacatcattc agagcatcga ccggaaaatg gacctcgaag acatagccaa
    14281 cgcaaaaaat ctcgaaatgg atgagctgct gaacgaaatt gaggcaattg tcaactcggg
    14341 taccaaaatc aaccttgact actacatcaa ccaggttatt gacgaggata agcaggagga
    14401 aatattcgac tacttccgca atgaggccga aaccgaaagc ctcgaggaag ctcttgttgc
    14461 ccttggcgat gaggattaca ccgaggagga aatcagactt atgcggatta agttcctttc
    14521 cgaaatggga aactaattgg cccgaactac ccgcccacaa cctcatttta cctgtaaatc
    14581 acaatgtaag cggcatgctc caaaggtaac aggggcagat atagctgttt gccgttgctg
    14641 ctgaccaccc acaccaaagt acaacttttt ttgccatacc atcaccaaac gccacattgc
    14701 ttcacatcca agactcttct accagcctga gaaaagcatt ctgttaactt ttcaactcac
    14761 taaacccaac ctctttgctt atggagctgc ccttaaagaa gttaacaaaa aattaaccgc
    14821 acaacgtatt gatttcattt cgaagttgag ctatatttgg gcatagctaa acctaaaagg
    14881 aggccgtcat gaagaaatta ttactgctca gccctcagct ggttcttacc ctaagtttat
    14941 tcgggcaaac tattattacc agccaaaacc acggattcat tcctaatgac aataacccaa
    15001 tgctgcttac aagctacgta gaacccggcc cagctggtaa aaacgtggtg tggaacttca
    15061 gcatgctgga aacttcggag aacttccagg gcagcctaaa agaggtttac accctcaagg
    15121 attacccctc aaaccagggt ggcaacgtgg tgcttgaaga gttcggcaat aggtttatct
    15181 tcaatatgac agccaactca ctggagcaac ttggttttca gtcatctaca ggaagcaccg
    15241 aaataacata cagcaagcca tttgtcaaaa tgcgctaccc gtttggtttt aacagcagct
    15301 tttccggaga ttttaagggt gattaccttg ttaacaaagt gcctgttggc accatttcag
    15361 gaacctaccg ggtggagggc gatggctacg gtaagctgat tttacccggt ggaagagagc
    15421 taagtggagt tttacgggtt aaggaggtga agctcaccac gcaaaccatc aacaacagcc
    15481 tgattaacat tgaggatgtt acctatcgct ggtatgtggc caaccaccgc tttccggtac
    15541 tatcgctaat aaaaagcacc tacactccgg caaacgggaa accttcaaca caaaccaagg
    15601 cagccttcaa cggcaatgtt gtaagtcaac caagcgatgt ggacaaggtg tcggataacc
    15661 tgctgaggtt tacaacattt ccaaatccct ataagggcaa ggttgccatt acctacaccc
    15721 tccccgaaag ctgcaaagta aaccttgcgg tgtatgacat tacgggcaag cttgttagcg
    15781 ttttggtgaa cggtattgag gatgcgggac aaaaaaccca ctacttttcg gcagcagaag
    15841 taggaatgcc tgctgcaact tacatcgtta agcttacagc aggaaatgtt gaattcattg
    15901 gcaaggtgct tgaggtgagg taaacaggta gttactgatt tttctgctgc cagtccctgt
    15961 tccatgccag gtatatctta aacgaaacgc catggttcag gtagtagttc aaaaagtcga
    16021 aacctaaatc ctcggcgtta tcggctgctc ctttaaaggg cgcattcatg gcgccttcaa
    16081 agtcgtatcc cagagcaaaa attactaccg ttggcccggg atgaatgtag tagcgcaacc
    16141 ccaaggagag ggagtaatga accattacat tgaaaaactt actggtaact gcagttctac
    16201 ccgggttaag cgcttcggcc atagaaactc cgtaatcgga tattttggcc ggcccaaaac
    16261 caaccttatc ctgcgtggag gcatacatgg caaaacgcat ggcgcgtctt cccggagcaa
    16321 aaccttcaag tcctcctgta aggcaaagct gtcgaagcaa gtcaaatccg aaacaaatat
    16381 tatacgattt aatggagtag agcgtatcat aagcaggcat tccaaacttc tggttctccc
    16441 ttccgccttc ggtaaccagg gtgctaatct ctaccggata acccattgag gtatagccaa
    16501 caccccagta ccctccaaca tcgttaaacg ccgaaccact tttagcagtc tttaaaagat
    16561 ctatgttgaa gtacttgttg gaaaattccc tattggcata gtaaccagga ggcagcgctc
    16621 ccgaccattc caggtcgccc gacatcttgg aactctgtag ccgtagtagc atgttcttaa
    16681 atcccaagta cccgccccag cggtaaaact tacctattag gttgttctcc gtctgggccg
    16741 acacccccat cctgacccct agcctacgaa taccaaaatt gccctcgtag gacgacatgc
    16801 tggtcatgtc aaaattcagg gaaagaggtt cgccgataga tttatggttg atgaagtaca
    16861 ccttccatct cagctccgga atgtatgaga cacctagata ggcaggtagg ttattctgcc
    16921 cgtatgcgct aacagataat atcactccgc tagccaccaa tagcattagt agttttccgc
    16981 gcatcctata aaaatttaat atgctaagct ataaaaatag aagtaagatt catatattcc
    17041 ataactcctt ttacaaacta cagtaaaaat gtggcaaaca gccaaattga agtaaccaaa
    17101 cctgaatgcg aaaagaaaat tcaatagtta tcaacatcca ttttacctac cccatatttt
    17161 ggtaaattcg cacgataaca ggtagggtta atggctaagt tcattcattt gaatcccaag
    17221 attttcaatt taaaatcacc ttcgttttca tgacggtaac gcttcatcct gccatttcgt
    17281 aaatctgagt attgactaca attaagtaaa tgggaagttt tgtacatctt catgttcaca
    17341 gccactactc catactggat ggagcatcga gcattgaaat gctagtaagc agggcaaaag
    17401 agctgggcat gccaggaatt gccataaccg atcacggcaa catgttcggc ataaaggagt
    17461 ttctgaatac tgtaaaaaaa agtaatagcc ctgttcttaa ggaaatagaa agcataaaag
    17521 ctgaaattaa aggcataagc tccggaagta gtgtggctga taacagccct tctctagacc
    17581 tgctgaacga gcagctgagg gaggccgaaa aacaactttt taaacccata gtcggctgcg
    17641 aaacatacct ggcaaaaaac agccgtttcg accgcacgga caaggaggac cgccataact
    17701 ttcaccttat tcttctggcc aagaacctgg aaggttacca caacctggtg aagctggtta
    17761 gctatggcta caccgagggt ttctactaca agccccgcat tgataaggag ctcctgcgac
    17821 agtacagcaa ggggctaatt gcctccagcg cctgtttggg aggcgaaata cctcaagcca
    17881 tacttaacaa caacatcgcc gaagccgaga ggctaataat ggagtacagg gatatttttg
    17941 gcgacgactt ttacctggag ctcatgctcc acaaatccgg tgaaccccgc attgactccg
    18001 aggtttacga aaaccagctg aaggtaaaca aggcattgtt agagctgtcg gtaaaaacag
    18061 gagtaaagtg cattaccacc aacgacgtcc acttcgtcat gtccgatgat gccgcctcgc
    18121 acgacctgct aatatgcctt aacaccgaca agcggattga cgacaccaac cgtatgcgct
    18181 acacctttca ggagtacctg aaaagcgagg aggaaatgct ttccatcttt tccgagcatc
    18241 ccgaagcagt tcacaacacc atggaaatac tggataaggt tgaattttac gatcttagcc
    18301 acaagcccat tatgcctaac ttcccgcttc ccgatggttt caccgatgac aatgattacc
    18361 tgcgccacct tacctacgaa ggagcaaaat tgcgctgggg cgataccctt agcgacgaga
    18421 tcggcagtag aatagagttt gaacttcagg taattgaacg tatgggctac cccagctact
    18481 tcctgattgt ttgggacttt ataagggctg cccgcgaaat gggggtatcg gttggtcccg
    18541 gacgaggttc ggccgctggc tccgtagtcg cctactgcct aaaaattacc gacatagacc
    18601 ccataaaata caacctgctg ttcgagcggt ttctcaaccc cgaccgcatc tccatgcccg
    18661 atattgatat tgattttgac gaggatggcc gggaggatgt gctccagtac gtggttcaaa
    18721 agtacggcga aaaacgtgtg gcccacattg taacctttgg caccatggca gccaaatcgt
    18781 ccataaagga tgttgcccga gtgttaaacc ttgacctctc aacctccgag cgtttgtcga
    18841 aacttgttcc cgaacgcatt ggcattaccc tgccggctgc cattaaagag gttcccgagc
    18901 tggaaaagga gctgaaatcg gacaacgagc tggtgagggc aacactaaag cacgcacagg
    18961 ttcttgaagg aaccatcaga caaacaggtg tccatgcctg cggcatcatc attggacgcg
    19021 acgatttgga aaactacatc cccctttgta tcaacaagga ggccaaactc ttcgttaccc
    19081 agtatgaggg caaccatatg gaggacattg gcctcctgaa aatggacttt cttggactga
    19141 aaaccctttc cattgttaag gatgccctgc agcttatcat ggaatcaacc gggaaacagc
    19201 tcgatataga caatatcccc ttggatgatt cgacaacact cgaactcttc tctcggggcg
    19261 ataccaccgc cattttccag tttgagtcgg ccggaatgaa aaaatacctt aaagccttgg
    19321 agccaaaccg attcgaggac ctcattgcca tgaatgcgct ctaccgcccg gggcctatgg
    19381 agtatatccc cagcttcatc aaccggaagc atggtcggga gaaaatagag tacgacatac
    19441 ccgaaatgga ggaattcctg aaggatacct atggaataac cgtttaccag gagcaggtga
    19501 tgctactctc gcagaaactt gccggattta caaggggtga ggctgattct ctgcgaaaag
    19561 caatggggaa aaaactccgg gacgtcatgg acaaaatgaa gggaaaattt atccatggat
    19621 gcatcaacaa cggacacgac caggctaaag tggaaaaaat ctggaccaac tgggaagcct
    19681 ttgccgaata tgccttcaac aagtcacatt ccacctgcta tgcctttctg gcctaccaaa
    19741 ccgcatacat caaggcacat tacccgggtc agttcatggc ggctgtgcta agccggaatc
    19801 tttcggatat taaaaaaata accactttca tggacgagtg ccgtcgaatg ggcctgcaag
    19861 tgctgggacc ggacgtgaat gagagcaact acaagttctc agtgatgccc aacggcgaca
    19921 tcagattcgg cttgggagct atcaagggaa tgggcgaaaa tgcagcagct aacattatcg
    19981 aaactcgcaa ccatgagggt aaatttaaaa atatatacga tttcatggag cgggttaacc
    20041 tccaaacagt taacaagaaa aatttggaat cgctcgtgct ggctggcgct ttcgacaaca
    20101 ttagccccat tagccggggc gcctatttta cacccgactc caagggtgta accttcatgg
    20161 aaaccctgat gcgctacgga gcaaaggttc aattggaaaa aaacagtaag cagatgaccc
    20221 tttttggtgc aaccaacgga tttgaggtac cacaacccga acctcccaaa accgacgatt
    20281 ggccaaccat cgaaaagctg aacaaggaaa aggaaatcat tggcatttac ctctctgctc
    20341 atccacttga cgagtttcgg tttgaaattg agaacctatg caacaccaac ctggccgaac
    20401 ttaaaaacct cgaagatttc aacaacaggg aagtaaccat tgccggtatg gtggttaagg
    20461 ctaagattga tactaccaaa aatggcaaac cttacgccag gctcaccatt gaggactact
    20521 ccgacaccat ggacatcatg ctgtttggaa aggactttga aacctatcga aatttctgct
    20581 acaccggtta catggtgatg attaagggaa aagttcagcc caaagccttt aaaccagagg
    20641 agttggagct tcacatcaaa agtatgagca tgctttacga cgttcgggaa aaacaaataa
    20701 agggtatttc gattaccctt cccaccactg aaattaacga catacttatt gacgatttta
    20761 ccaggttaac ccacgagaat gccaaggtat cgcttcgatt taaggtggta gatgtggaaa
    20821 acaggatttc ggttgacctg ttttcccgat cgcaccgggt tagcatcaac accgaactta
    20881 ttcacttcct ggcagataat gagctggagt ataaactaat tacaagctga acctttggta
    20941 ttaattttcg ttttaatttt gtactggtaa acgaattaga ttatcaattt aaaacaatag
    21001 ttgtcatggc acaagaagta aacgattcca actttgacga ggtggtgctg aatgccggca
    21061 aacctgctgt agttgatttt tgggcagaat ggtgtggccc ctgccgaatg attgccccct
    21121 acatcgaaca aattgccgat gaatacaagg atagagctgt tgttgcaaag gttgatgtgg
    21181 ataataaccc tggcattgct gcgcgtttcg gcatcaggaa tatccctacc gtgctgttct
    21241 ttaaagatgg acagctggcc gacaagcagg ttggagctgt tcctaaaagc aacctggttt
    21301 ccaaacttga agccctactc aggtagtttt tttaaaaatt ttcataccac catgatgttt
    21361 agggttatta acccgatgtt gtggtggttt tacctatttc tactaaacat cttcattatt
    21421 atctttaccc aaaatccccc ttccaccatt cgcgttgcag caaatggagt tttcaataga
    21481 ttctaaccgg tattctccat cagctgtgca ttacaaacag cggcattccc gaaataaagg
    21541 actaaccgca tgttcttgca aacgtagagc ccatttaatc ttttccttat gagaatttcg
    21601 gtttactgtg cgtccagctc aaaaattaac caaaagtatt ttgatgcaac ccgtcagcta
    21661 gcctcggaat tggccaacag aggcgacatc atagtgtatg gtggtggttc tcacgggctt
    21721 atgggatgtc tggccgatac cgctcttatg aatggaggca tggtggtagg tatattgccc
    21781 cgatttatga ataaagttga gtggggacat aagaatctca ccgagctaaa gctggttaag
    21841 gatatgcatg agcggaaaaa gctcctcatt aaggatgttg atgccgtagt cgccctgcct
    21901 ggcggctgtg gaacgcttga ggagctgatg gaggtaatca ccctaaaaag gttgggaaaa
    21961 tttacgaaac ccataatcat tctcaatacc gatggattct acgattacct aaaattactt
    22021 ttggccaaga tgattgatga gcacttcatg cggcctgaac atgcaggtat ttggacattt
    22081 gtggaaaccc cccaggaggt tattccggcc atagaaaaag cgccaacatg ggattcggat
    22141 gctattaaat ttgcagcact gtaacttttg caacgcggtt acgcctggca ctgcaaaact
    22201 acaatggtaa atttgaaacg atatgctgaa ggttattagt atttcacctg ttgcttcttc
    22261 cctgctgatt ccgataaatg aatagcttgc tgtcccctac ctcatcgtag gatacaggat
    22321 cacccttaaa aagaggggcc ttcactccct cgccaaaaaa gcggttgcca acaaataccc
    22381 gtgcctcgtc ccataagttt ttctgaatga agttgttgag cagcgttgca ccgccttcaa
    22441 ttatcaatga aataacatcc cgatcgctca gctcttttag gatttgctgc tcggagcctt
    22501 tggcaaagtc gattgttacc atctccaggt ttgagatgcc tgcaaattcc gatttacgca
    22561 ccagcgcgct gctgttattc ccaatgaaca aaattgtagg ctgacttcca tcgaaaacat
    22621 tggcatccct actcaggcgc aactttcgat caacaatcac cctaatggga tttcttccac
    22681 tccagctgcg gatgttcagg cgtgggttat ccctttcaac ggtgttggtt ccgacgatca
    22741 ctgcctgctc ttcgcttctc catctatgta ccagcgagcg tgccagctcg ttagttatcc
    22801 aaacagaacc aacaggtgtt tcagcagttc taaccagatc aataaatcca tctatagttt
    22861 gagcccattt aagaataata tagggtcttt tgcaggtatg aaaggtgata aacctcctgt
    22921 ttagcagcaa tgcttcatcc tcaagaatac ccagagaaac ctcaactcca tgcttacgca
    22981 gctgctccaa cccgcggccc gagaccctgg ggtttggatc ggttgtggcc accactaccc
    23041 gcggaatgcc atactcaaca attcggtcgg tacatggagg agttttacca taatgcgagc
    23101 agggctcaag cgaaacgtaa agcgttgaat cctgcaacag cgatttatcc ctaaccgagt
    23161 taatggcatt aacttcggca tgtgcctcac catactgcgc atgaaatccc tcaccgatga
    23221 ttttaccgtt gtgtacaaca acagcaccca ccatggggtt tggagcaaca cttcccatcc
    23281 cgtttaaggc caactccagg cagcgagaca tgtaaagctt atccacatcg aggttgtcca
    23341 taaatattac ctaaatttgt gctatgacaa agttaaattt ttaaatgaag agttacactc
    23401 aaaggcagct atttgatgag gtgggcttag tcctgaacca attttttact tccggtgagg
    23461 taaactcctt gcgtaggatg ctgctggaac atgtgcttaa aattccctac taccatgttc
    23521 acctaacccc aaacgcccaa gtcagcccca gcctgtccaa aaatatatta gacatgacag
    23581 gccagctggt tcgaggcgtt ccaattcaat acttgctggg cgaaaccact tttatggagt
    23641 taaatctaac tgttaaccca tcggtgctca ttccccgccc ggaaaccgaa gaacttgttc
    23701 gatgggtgat tgctgattgc cacttaccaa aacctaaaat tctggatatt ggaaccggct
    23761 cgggttgcat tgccattgcc ctggcacaaa acatccccga ttctaccctt acagctgttg
    23821 atatttcccc ggacgcgctt aaagttgcaa ggcataacgc taaaatatac agcgcctcga
    23881 tttgctttgt agaggctgac atcctttccg gcagcctgat tccaaacgct cccttcgaca
    23941 ttgtggtgag caacccaccc tacgtgcgca gctccgaaag gaggtttatg cacaaaaatg
    24001 tgctcgacca cgaacccccg ctagccctgt tcgtaagtga tgataacccc cttcaatttt
    24061 acaaggctat tgtaagcaag tcctccaagt tgattaagca agggggaagg ctattcctcg
    24121 aaattaatga ggagtttggc aaccaaatgg tagagctgct ggaggcaaac cattttggca
    24181 aggttacgtt gcgaaaggat ataaatgaac gctaccggat gatttcgggc acctataccg
    24241 gctaaacata accaacccac tgtaattatg gatagcagag aaaaagtgag tattgataaa
    24301 gctttaacac ggctccaaaa cctatgcgca aggcaggaaa agtgcgtgtg ggatatcatg
    24361 cagaaactta aactatgggg gattaataac gaagaggctc aaaagattgt ctccatgttg
    24421 gttgatgagg gatttgttga tgacagaagg tatgcacaaa tgtttgtaag ggaaaagagc
    24481 cagctaaaca agtggggcag cattaaaatt gcagcagctc tgcgggtaaa aaaaattaac
    24541 gaatctataa ttaaggaggc gctgctcgag cttcagcccc aattcgaccg ggcaactctg
    24601 gaaaatatta ttaccacaaa ggccaaccag gttaaggcta aaagtccgtc ggacttacgg
    24661 gcaaaacttg tccgatttgg gttgtcgagg ggttttgaac tggagcatgt ggtaaaaacg
    24721 gtggacatgg ttatgaaaag caatatgaca gatgaggcct agctctactc cactatgaat
    24781 aaacggaaaa gtcagcattg gagcaccaag ccaaagtata tcatattgcg caacagcaga
    24841 aaaaaattga gttagcgcaa aggtaaaaag tagaactttt tcacctaaaa aagggttact
    24901 aatattaagg tggtaagcaa caaaacaggt aacggataaa tagcggttta aagatgctaa
    24961 agatttttac aaggtttgat tccaaaaacc atcgtcccgt gatgggagcg atggaaatat
    25021 tcaagtttat tggacccgga ctgctggtaa ctgtgggatt tattgatccc ggtaactggg
    25081 cttccaacct cgcggcggga gccgactatg gctataagct gctatggatg gtgagcctga
    25141 gcaccattat gctcatcgtc cttcagcata acgtggcaca tttgggaatt gccaccgggt
    25201 tgtgcctgtc cgaagcaacc acaaagtacc taaaacctgg cgtttcaagg tttatcctgg
    25261 gcaccggaat gctggcatca gtctcaacct cgctggccga gataatggga ggcgcaatag
    25321 cactacaaat gctattcggc atacctatca agattggcgc agtgcttgtt ttggcgtttg
    25381 tgctagtgat gctattcacc aatagctacc gggcaattga aaagtggatt atagcatttg
    25441 tctccataat aggactatcg tttatctacg agttgagcct ggttaatatt gactggcccg
    25501 aagccatcaa atcctccgta acgccatcgt ttcctgaagg ctccatgctc gttatcatga
    25561 gcgttctcgg ggcagtagtt atgccccaca acctgtttct gcactccgag gtaattcaaa
    25621 gccgacagtg gaaccttcag gacgacaggg tgatacgaag acaactcgac tatgaatttt
    25681 tcgatacact tttcagcatg ctggttggat gggccataaa cagctccatg atactacttg
    25741 ctgcaaccac ttttcataaa acccaaacgc cggtttcgga gctaagtcag gctcaaaaca
    25801 tgttaatacc attacttgga agcagtgcag ccgatatatt tgcagtagct ctgctacttg
    25861 cagggatatc ctctaccata acctcgggga tggctgccgg atcaatattc gcaggaattt
    25921 ttaacgagcc ttacgacgta aaggacaacc attcaaaact tggaattctg ctttcgctgg
    25981 tagctgctgt gctggttata tttgccatag ataacccttt taaagcactg ctaatatccc
    26041 aaatggtgct aagcattcaa ctacccttca cgatattcac ccaggttagg ctcacctcct
    26101 ccgaaaaggt gatgggaaag tacaaaaaca gtccctttac aaaatggaca ctttacggtc
    26161 ttggagccgt agtaaccctt ttaaacctaa tgcttttcgt aagctttcta ggataggttg
    26221 aagtattagc ctgattgtcc tgcaaagagg cacggaatgg aagcccgcta ccacacacag
    26281 ctattcaaat ttcgtggcaa ttttttgggc aattgcatca agctgttcag gattcagctt
    26341 tagctttggt ttatggaaat caatgtccga ctcggaatta attggtatga ggtgaatatg
    26401 cgcatgaggc acctcaagcc ctaaaaccgc tacaccaata cgcttacatg gaatcacgct
    26461 ttcaattgcc ttggcaaccc gctgggcaaa aaggactaat cccgataggg tttcctgatc
    26521 gaggttgaaa aggtaatcaa cctcctgttt gggtattact aaggtgtgcc cttcagccaa
    26581 agggttaatg tcaagaaagg caaaatattt ttcgtcttca gcaaccctgt aagagggaat
    26641 ttcgccattc acaatttttg agaaaatgga agccataacc cgacaggatt aaagtgatat
    26701 gctaagaatt tcaaacttca tcagacccga agggacctga acctctacta tgtcgcctat
    26761 ttttttacca atcagcgctt tagctattgg cgtggcgata gagatcttgt tctcgcgcaa
    26821 gttagcctcc gactccgata ccagcatgta ctcaactaca gcatttgttt tcagattttt
    26881 aagcttaacg cggttgagta tttgcacgcg ttcggtatca atcttcgact catcaataac
    26941 cctggcattg gcaactaggt cttcaagctt tgaaatacga agctccagca tgccctgggc
    27001 ctccttggct gcatcatact cggcattttc cgacaaatcg cccttgtccc ttgcctcggc
    27061 aatcattttg gaaatggctg gcctctccac actctttagg tggtcgagct ccttacgcag
    27121 cttctcaagg ccttcttcgg ttaagtagtt cactttggac atttcaacct ccttatacat
    27181 ctgaaataaa aaagaattcc gattgctcgg aactctcatc ttttgcaaat ataacaaatt
    27241 ttagaataaa aatctcggaa tgaaatgaaa gctaaaaata ttttttatta attacctcag
    27301 agtagattac tgccttggcg ggatcaaaat catccaaaat gccagcttgg ttcgattcct
    27361 gctcaagcac tttttcatac tcaaaaatca tatcgggggc tttagactct tcctcttgct
    27421 cctcgaaagc aataggctga tagctagaag ctagaccctc gtaggttgtc tcgtagggac
    27481 taacaggctg gtcgagtagg ctgcttcccg aagaagtggg aattttatcg agctcttcat
    27541 actttttagg ggcgtacaac tcctcttcgc ttacctgcac tgacggcatc tcatactcca
    27601 cagatttaac ctcaccctcc atattgtccc agccagacaa cacagacttc ccttcatttc
    27661 gcgggtttga attagaagta aaatcctgca tagtgtcgtt ggacggaaac tcgcttactg
    27721 attgctgagg ccatgtatat atgtcttcat tattattttc aggtatttca actggttggc
    27781 cttctgaata gtttttaggc tcctctgctt cggtctgctt catggcccgt ttggccgcct
    27841 cttttttctt cctggaaacc gagctgataa ttcctaaaat ggtaacaatg attatgaata
    27901 ggatatctcc accatccatt tcagcaaata tttagttaac aaaaattcaa ctacccacac
    27961 taacgtcatt tccgttaaat tgttacgaac ggcaatccgt tgctgttttt caccatcagg
    28021 tttaagccgg tattataagt aacaaaaaca attgtatttt tgtaatgtat ttaccggaat
    28081 aaaaccatga gagcaaagat agctaaactt ttagtaatag cagcatcctt ttttttgcct
    28141 agcagctgtg gagaggacga gcccgaaatc gtccccgatg tcccagttca catagaattc
    28201 taccttaacg aaccctttta caacaacata accgttccca tgggcgcggt aaaaattcct
    28261 aatgagggtt acaacaacaa cgggattata gtattccgtc taaaccagga ggaaatagtt
    28321 gcctttgatg ctacctgccc gcagcacatt gcagtaaatg catcggtggc ccttgatgaa
    28381 aacggctcag gaactgccac ctgccctcat tgccatacgg tttactactt ctacaataat
    28441 ggctacccaa ctaaggggta taagctgaaa tcataccaag tatcattcag gggtgatttg
    28501 gtgggaatat acaactaacc cgctctccag gactttcttc ccgcattact ctactaccta
    28561 aaataaacca gaaaggtttg aaatttattc atcattctca ttttaggaat aatttttgct
    28621 taaaaaaatt ttctaacaat acaatttttc tcctaaattt gaatataaaa tgttacctaa
    28681 catgattcca cccgtaaaac atgcaccccg ctggttggtt ttcgtcattg atttatgcat
    28741 ctgcctggtt tccattactc tatcgtacta cgtgcggttt gagtttcgga ttggaagcga
    28801 gcaagttaat gccctaaagc tagttgctcc gctagtcctg tttgtaaggg cgcttgcatt
    28861 tatttcgttt cgtacctacg caggcttaat tcggtacacc agtttgaaag atgctgaaag
    28921 gatattcatg gtaaccactt ccagcacaat agtactgttt accatcaact ttatagttta
    28981 ctctaaatcg ggccagttcc taattccaaa ctccatcctg ctcatagatt tttgcggcac
    29041 caatattctt atgattggct ccaggctatt ctacaaaacg ctttattata ccattaaaaa
    29101 tgttgatatt gaagccgaaa atattgtgat tgtaggcatt gaacaatttg ccgcttcagc
    29161 aaaaagggca attgacaagg acaacatggc tggtctaaag gtggtggcct ttattgaccc
    29221 aaactgcaag caggagggaa actccattga ggatgtcagg atatacaaca cctcaaaaat
    29281 tgaattcctt gttcaaaagt atggcgtttc aaggatttta atcgcccaaa aaaatcttga
    29341 ccctgtaaaa aagaacaaaa taatagacaa gtgcctgcag ctcaacgtaa aggttcaggt
    29401 tatacccgat gctaccgcat ggattaacgg agagcttaac accagccagc taaaaagcat
    29461 aaacattgaa gaccttttag agcgccctcc tattgttctt gacaagagga gaattcaaaa
    29521 ctacataaag aacaaggttg tgctggtaac gggtgcagcg ggatcgatag ggagcgaaat
    29581 ggttaggcag atctccaaat tcagccctaa aaaaatagtg ctcttcgacc aggccgaaac
    29641 tccgctttac gatcttgaac tggagttaaa ggaaaacctg cacttctacg actttgaatc
    29701 tgtaattgga agcgttacca atgactatag ggtaaataaa ctctttgatg tttttaaacc
    29761 caacgtggtt ttccatgcag cagcctacaa gcatgtcccc atgatggaga ataaccctac
    29821 tgaggctgtt ttcaacaacg tgctaggcac aaagctaatt gccgaaaccg ccgtaaggta
    29881 tggagtagag aagttcgtga tgatttctac agacaaggcg gttaacccca ccaacgttat
    29941 gggtgcaagc aagcgcatag ctgaaatctt tacccaatcg ctcagctgcc aggttgatac
    30001 caactttatt accacgcgtt ttggcaacgt gcttggttca aacggctcgg ttataccccg
    30061 tttcagaaaa caaattgaga agggtggtcc ggttactatt acccatcccg aagttacgcg
    30121 attctttatg accatcccgg aagcttgcca gcttgtgctt gaagccggag ccatgggtaa
    30181 tggaggcgaa atttttatat tcgacatggg cgaaccggtt aagattcttg atctggcaaa
    30241 aaaaatgatt aagctctcgg gactcaccct gggcaaggat atacaaatta acttcacggg
    30301 gcttcgccct ggcgaaaaac tttatgagga acttctaaac aacactgaaa acaccatacc
    30361 cacctatcac cccaaaatca tgattgccaa agttcctgat ctgatccccg aaaatgtcat
    30421 tccgaaaata aacaggctaa ttgaaactct acccgaacac aacaacttca aggttgtttc
    30481 actgatgaag gatattgttc cggagttcat tagccgcaac tcgatttacg aggatatcga
    30541 tcgtcaaaaa aaatatattg ttaatgacta taaaaccgca tagctacttt cactcaaccc
    30601 ccattcagat aagagttaac gatattgacc cgctgaacca cgtcaacaac tcggtttacc
    30661 aacagtactt cgatctgggg aaaattgcct actttaatag ggtgctggga gaacatctaa
    30721 acttggaaac agagggcttg gtgctggcca acattactat tgattacctg aaacccataa
    30781 ccctttacga tagcatagag gtgtttacca gaatctacga aatgggaact aaaagcctca
    30841 aaatgggaca ggagattgtg aacagggtat caaaagaggt ttacaccacg agcaagtcgg
    30901 tgctggtttg cttcagcaac tccagtggac tttccatccc gatcccttta ctttggaggg
    30961 agagaattgc tgattttgag aaagaaggcc taatccatgg agttaacctc cagtaaacct
    31021 tgaggtatat ccatccaaac aattcgttca gcatcatcta ttcttgaaat caaattttca
    31081 gcggcaggga taagcagctc ctttcccgat tttgccgtaa cggcaaaaag ggcattatag
    31141 gcaaactcct cgtagcgggt aataatgcca agctcctcat ttttggtact gaatagccgg
    31201 taacccacta gggatgaaaa aggaacaccc ccatcatcat ttgataaaca gccacagggt
    31261 aaaagaacac ggaggccaac cagctcagca gcctcctcaa ccgaatcaac ttcgtcaaac
    31321 ttaacaatca aaatctcgtc ggaaatagca actataccct gaataaaaaa aggaactggt
    31381 attccttcaa tatcaagaaa taccaattcc aactcctcgg gtaaaacgcc agctgaactc
    31441 gctactgaaa tctggatttc acctttaaca ccatgagttc tctgaataaa gccaacctca
    31501 acaagctgag tatcggtcat gcattactat tcttcactgc tgccagcctc agtatcagca
    31561 tttgcttcca cggcatcctg agcagaattc tcggaagatt ttttagcttc cttttcagcc
    31621 tcggcagctc gttttttagc aaccgcttga gctctagcct cacgtacctt ggcttcggct
    31681 tcaagtcgtt cattctttcg tttagcgttg ctgttttcta cgaggtcaat cttcgcctta
    31741 atcttgcctt ccttctcctt gagccacgta tcaaacttgg cttctgcctg ctccatggtt
    31801 attgcaccct tttttacacc aatgagcagg tgatttttat aaagaacacc cttgtatgaa
    31861 aggatagccc tacaggtgtc ggtaggttga gcgccttttt gcagccagtc caatgcccta
    31921 tcaaaattta attcaatagt agcaggattt gtcagaggat tatatgaacc aatcctctct
    31981 atgtatctgc catcccgtgg cgccctgcta tccgccacca caatgtggta gtagggattt
    32041 tttttcctac catggcggct tagtctgatt ttttaggcat acttaacgct tttttaattg
    32101 gttaaaccta cttttttgcc ccgcaaagat atgctttttt attttattgc tctcatacta
    32161 ctgtaaatca ttttttatga tactcgtcag cgctcaataa tgcaaacgct actattgcca
    32221 aacacggatt actttaccat aaactctatg gaacttataa aaaaaagtat cacatttgca
    32281 ggctaacaat tatatacccc aaacgcaatg ccttttaacc gagataaagt tgagcaatgg
    32341 tctctggcat actggttgct caagtgccac gagaattttt tctttaagct gtactttaga
    32401 actgaaatag ttgggattga gaaacttcca gataatgcca ccctgatttt tgcccccaac
    32461 caccagaata cccttatgga cgcccttgcc atccttacgc tgaaaaggga ttttcaaccc
    32521 atatttctgg ccagagcgga tatattcaaa aagcctttcc tttcaaaaat tctcacattt
    32581 cttaaaatta tgcccgttta ccgttcgcgc gacgggtttg aaaatctcct tctcaacgac
    32641 aggatattcc aaaagacctt ggatgtcctt caaaaccgta atgggttggt ggtgcttccc
    32701 gagggaagcc atgagggggt taaaaagctc cgccagctga agaaaggaat tgcccgtatt
    32761 gcctttcagg cagaggatgc cgccaacggt aaacttggta ttcagatagt acctgttggg
    32821 ctagagtaca gcaactacgt agctttcaga agcaaccttt taatcaggct gggtgaaccc
    32881 attgaggttg accgctacct tgagctctac aggcagaata gggcacaggc ctacaacgct
    32941 ctaattgccg agctggagca acgcatgagg ttggaaatga tcaacataga ggatgaaaac
    33001 tataccgcct accttaccat cctggagcta ttttcagcaa ggtacattaa ggagagaaaa
    33061 ctacctaagt cacaaaacca gaaatttcag gttgataaga aaatcatttc actgctagac
    33121 aggctgaaaa atacaaatgc ttcggaattc gaggagataa tcaaaaatgc gaatgattat
    33181 ggcaggataa taagtgctaa tgggctttcc gtaaaactcc tgcctgcttc ctcaaaaacc
    33241 cttcgggatt taccaatcag attgctcttg ctgctaatta ccatgccagt tgccctgtat
    33301 ggggcgggaa acaacgctat tcccatagct gtcccctacc tgctcagcaa aaagttcaag
    33361 gatgttcagt ttcacagctc tataaaacat gccggggcgt tgcttttaac ccccctggca
    33421 tatctagtac aaaccctggt tgttggattt gtgctgaaaa acatttggct agctttggcc
    33481 tacctggcca cgctacccct tggtgctgct gccttttacc tatggcgaag ggagttcttc
    33541 cgatttacct cccagttatc gctacgaagg ttccgaaaag ctaaagctga agagttcgac
    33601 ttggttaacc atcttaacaa ccagatttac agaactgtaa caggtttggt tgacaaagaa
    33661 taggctacaa aacttcccat tctccaagat caagatttag aaaaggcttt agtctctaaa
    33721 tggccaatat gggccacatt taggtatgat ttttttcacc gcaaggcatc actaaaaaga
    33781 attaaggcag ccttgttgag ccgccttaac cattatacct caaaaatact gcttactact
    33841 tatcaagctt gtgctgtttc acaaatttct cgagtactcc tttgaggtcg ggaagaccaa
    33901 tttcaaccaa atcgtagtaa actcgggtgc ttggtttctt tattttgata atgcgattaa
    33961 ggtctattgg tgtgccaata atcacgctat cgcaatcggt agcgttgatg gttccttcca
    34021 aatctttgag ctgctgctca ccgtagccca ttgcaggaag aatccggcca atgtttgggt
    34081 aaatctggta ggtttcctta agctttccaa cgaggaacgg acgggcatcc acctcttcgg
    34141 aagccccaaa cttacgagct gcaatggtgc cggcacctat cttcatctcg ccatgggtga
    34201 gggttgggcc atcctctaca atgaggcagc gtttgcctgt aatcagctcg gggttatcaa
    34261 ctttaatggg tgaagcgcca tcaacaacaa tggccttggg gttaacccta cggatgttat
    34321 cacgaaccat ctggataccc tcaggagcag cggaatccat tttattaatt acaactacat
    34381 cagccatacg gaggttaact tcgccgggat agtagcttac ctcgttgcct ggacggtgag
    34441 ggtcggcaac ggttatggtc aaatcgggtt tgtagaacga aaagtcgttg ttgccaccat
    34501 cccaaaggat tacatcacaa ccgcttggat ccttttcggc ttcacgaaga atggcctcgt
    34561 agtcaacgcc agcatagata acattcccgc gaacaacgtg gggttcgtac tcctccattt
    34621 cctcaacggt gcacttatgc tttttaaggt cttcaaccgt agcaaaccgt tgaacctttt
    34681 gggcaaccaa atcaccatag ggcatggggt ggcgaacagc aacaaccttc aggccaagcc
    34741 ccatcaggta ctctataacc tttcgggatg tttgcgattt accgcatccg gttcgggttg
    34801 ctaccacagc aataaccggc ttagtgctct tcagcatggt ctcattgggc cccaacagca
    34861 taaagtttgc acctgctgcg ttcacaattg agctgatccg cattacattc tgatacttga
    34921 catcgctgta ggagaaaacg caatcatgaa cgttcttctc cttgatcagg cggggtagct
    34981 cctcctccga tactatggga attccatttg ggtaaagctt tcctgccagc tctgcaggat
    35041 attttctacc atcaatatcg ggaatctgag cggctgtaaa ggctacaacg ttgtactcct
    35101 ccttgttgcg aaagaaagtg ttgaagttgt gaaaatctct tccggcagcg ccaataatta
    35161 ctacattacg tttattcata aagcctcctt taaagtgttt actaaattta aggtttgtta
    35221 aaatccataa cgctcacaaa attaagtgca ttaagaaata attatttttc gttggaattt
    35281 ttttttaggt tcgtattttt cacaaaaaat gaaaattcaa gtcaaatgtc tgttattctt
    35341 atacttagtg atgttttctg aaaaacatgt taagaaacat gtaatggcag gcttgttcgg
    35401 tagccccttg ccagcagcaa atgtaagctg gcgaagtgat gccgtgcatg gagcgaacaa
    35461 cggggaaggt caaaataatt gcgagcagat ggaatgaatg ggggcttaat ctcacgaaaa
    35521 agggctgcaa gttgcagccc tttttccgca tcattacatc aatgcagctt actatttctt
    35581 aacaacattt cccgataaac gcagaaccgt ggttgaactt ttaggatcgt tggttatcac
    35641 ggtaatagat ttactctgcc tacccgaata cccattagtc ctgaacaggg ctttaatctc
    35701 ggttgactcg cctggtttaa gaatgttttt tgaaggttga acggttgtgc agccgcagct
    35761 ggctttaacc ttacgaatct gcagatcgct tttccctgtg ttcttaatgg taaagctgta
    35821 ctctacgggt tctccctcag ccttctggcc aaaatcgtag gaggttgtgt taaattctac
    35881 cctgggagcc ttttccagct cactggcgct gagggcggag taatcctcct caatgctgcc
    35941 ggtaatcgat attggggtgt ttgtaatcag ggaatcgttg atagaaagca taaacctgtc
    36001 cattacaaaa ccccagtcgt ttcgtttttg aggatcgtag gtaactataa tatttccaac
    36061 ttgtttaggc ttaatactct ggggctcaac ccttacggtt aagaaattta acccacctgc
    36121 attggcagca accttaacca cctcgcttga tttgttcata aatttgaggg tgtcggtata
    36181 aattttcccg gggaatagcc ggctaaaagg aatatgggtt ttttcaaagt atagcttacc
    36241 gccgatggaa atgttgaata tctcatcggg agttttaaca tggggaataa cctccccttt
    36301 aatttgtaga ataaccgtag gtgtttcggc atttgagtaa acagtaatgg ttttggaaaa
    36361 ggcccccgga cgccccgatg ggttatatcc caccttaatt cttccatttt tacctggggc
    36421 aatgggttct tttggccaat cgggtgttgt gcagccgcac gaggtttcaa cccgctgaat
    36481 tattagcggt gtacttcctg tgttggaaaa ctgaaactcg tagtatgcaa cgccatcgtc
    36541 ctcctttatc actccgtaat cgtgattgga atctttaaag taaatcctag ccacctgcga
    36601 ctgagcatca acaataccta ttagcagcat tgcacaggca aaaccggaaa tcaaaatcct
    36661 tttcatcatt atctacattt agtatgttgt aacttgactg cctcaaaacc tctcaaattc
    36721 aaaacaaacc atagttatgc tttaggctta tccatgtata cctggtaagc agaactcaca
    36781 caggttcaag tccttcaaaa cgaaaaccag gtaaaaatac ttcagcaata tcaatactca
    36841 atattttcaa aagctaaaaa atactgcaag caaaacgatt ttcacctggc aggtagtaaa
    36901 ccaaatcaac atcaaagtta cgaattacag gcaactacaa caattttttc aaagtaaaaa
    36961 agtgcgagcc tcaaacagta aaccaaattt tgacaaaaag gctttgaggg aaatgatcta
    37021 aacatctggc tttagtcagt aatgtaaggt tttcgtccgt aaaagtgctg atgatgaata
    37081 ttttttcgga aattacatgt aagtttgaat ggtttaacca ggttgcaaaa tgatggccag
    37141 cctaaatgac aagttaaagg aactctacaa gactactccg gttgttgctg gtgatattta
    37201 ccggcatatc caccaaaatc ctgaactatc gttccgcgaa tcaggcacat caagctatgt
    37261 tgccagcttt ttggaatcgt taggcatagg gataataaaa ctagataaga gctacagcct
    37321 tttggcaact attgacggca caggtgaagg caaaaccatt ggactcaggg ctgaactgga
    37381 tgcactgccc attgatgaga aaaccggaga atcgtacgcc tctcgaaccg caggagtaat
    37441 gcacgcctgt gggcacgatt ttcaccttgc gtcggttcta ggtgccgcaa taatcctcaa
    37501 caacctacgc gaattctgga gtggaaaagt ggtgctggtt ttcgaatcgg gagaggagga
    37561 acttcccggt ggtgccaggg ccattctcga atcggatgaa tttcaggagg taaaacccga
    37621 ctatatggtg ggcatgcacg tgctacccga acttaccacc gggaaggttg gcttttgccc
    37681 aggacgctac atggcatcgg gcgatgaggt ttatctaacg gctagaggac gagggggaca
    37741 cgccgcatta ccccacaccc tggttgatcc ggtagtcatt gcctcgacga ttattttatc
    37801 ccttcagaca ctggtaagcc gcaaggcacc ttccaacatc ccatccgttt tgtcgtttgg
    37861 ccgggtaacg gcaaatggct caactaatgt tataccagac gaggtaagca tttccggaac
    37921 tttcagaaca atgaacgagg aatggagggc aaaggcacac gatttaattg tccaaatggc
    37981 aagtgatatt gccagatcta cgggaggaga atgctctgtg gaaattcgca agggctaccc
    38041 gtcggtttac aacgacccaa tccttactca aacagccgtt gaggttgcaa gcagtatttt
    38101 cggttccgaa aacgttgctc ccctggagcc aaggatgacc accgacgatt ttgcctactt
    38161 cagccaggca atcccatcgg tgtttttcag aattggcgtt ggcacgccag gcgctccaac
    38221 cctgcagctg catcgtccgg atttcaggat tgacgagcaa gcgttccaaa cgtcgttacc
    38281 catgctaacc gggttgtgcc ttaaacttct aacatccagc taacaaactt ctgttggtag
    38341 ctatgctgtt gaaagccaaa cggctcaggg taaaccctaa gccgctcaac acttttcatg
    38401 cctattttcc gtgcatgaat ggcaacattc tagtaaactt caacatcata ggggattcta
    38461 gcctgcactc cctggttttc aaccatctgc ataaccctgt tgtagtactt atatcccttg
    38521 cccaaatcgt ccttcaccag catggcttta gcctcggttg taaacccctt aagtagctcc
    38581 tcgaagggcg atttctgctt aggcagctcc accaaccggt atatgtcgag gtttgccttt
    38641 tcggcggcca gctttatggc tgcagttagc ccgccaaaat catctaccaa cttaatttct
    38701 tttgcatttg caccgctcca cacgcgaccc tggccgatct cgtcaactct ttccacggga
    38761 atgcctcttc cctgagaaac atgcccgata aaggtctggt aggtttgttc aacaaaacgc
    38821 tgcccaacgg ccttttcctc ggcagtcatg gggcgaaaca tggagaaaaa gtcggaatgg
    38881 gcgttggttt tcaccacatc cacgttaata cccaactttt tatctaaagt tttttgaagg
    38941 ttgaagaata ggccgaagac cccaatagaa cccgttatgg tggttggact ggcaagaatg
    39001 gtggttgcag gtgaagcaat atagtaccca cccgaagcag ccacatcgcc cattgatacc
    39061 acaagaggtt tagcctgtcc ggccagctca acttctcgcc atatcacctc ggaggcaaga
    39121 gcactaccac caggagagtt gatgcggaaa acaatggcct tgatagtgct atcgcgccta
    39181 gcctgacgaa tggccttcga aagaccttcc gagccaattg ccgtattgct accatcaccc
    39241 atgccaatct ctccctgggc ataaataaca gctattttgt ttttggagaa agccgttttt
    39301 tgaacgggag gcaccttgaa gtatttctcc aaggtaacgg tattcaggtt acgattttcg
    39361 ggtattcctg taaaagcctt aagcttctct aacagctgat ccttgtatat caggctgtca
    39421 accagcttcg acttcatgca ggatttacca tcgtacacct ccatgttgtc ggccaggcgg
    39481 ttaagctctg caacagctat gttacgggat tttgaaattt catcaaccca actgccccaa
    39541 atagaggaaa ggtaggtaag agtttgctcg cgattctcgg ggctcatcct ttcactcatg
    39601 aatggctcaa cagcgctttt aaacttgcca tgacggataa tctgtggttc aacatccagc
    39661 ttttcaagcg cttttttgta gaacataatt tcggaacgca ggccgagcca ctccaccatc
    39721 ccctcggggt taaggtagat gctgtcggct acagaggcca ggtagtatgc tttttggcta
    39781 taggtatcac tatagctaac aacaaactta cccgactcct taaattccct cagggcctca
    39841 cggatttcgc cggttgttgc agcaccagca ttgaccacgt caaactccag caaaattccc
    39901 ttaattcttg ggtcatcctt tgccttggca attgctttta gtatctcatt aaggccaagg
    39961 tttttctggg gttcgaaagt tgccaggtcg agtgtttcaa aggggttgct gcttgcccta
    40021 tcaacaatct cgttttttaa atccatgtaa agaatagagt tgtcctttac ctcaacagct
    40081 ttatcggctg atgaaaccat gctgccggca atacccatca ttatcaaaaa tatcagaaga
    40141 aacgacagca gcacgccaac tatggtggca agcaagtatt taaaaaaact tttcatgata
    40201 caggtttttt agatgattac tactaaaagt tgaattttca gatttgctct gcaaacaggc
    40261 attccacctt ttaaaactca aacttataaa aagttatcaa aacaagcata aaatatgaag
    40321 caaaaaaaca aaagtgtagc tgtagtgggt ttgtcaatgt tgccgagctc gtgcggaaaa
    40381 atcatctgtt gtttgaacta atcagccagg cgtaaagcct gtgtatgcct actcagcaaa
    40441 tttttagtta atggaaaaat taaccaatcg cccctgagct aaagtttggg gtagctgcta
    40501 tgcgacaact gaacttcatt gttttagtag gacggaaaga atggaaggta ggtagtaaag
    40561 atttatccgc gaagtttata gcactgcctc aaaaacttgg cttatgggtt gtattccttc
    40621 atgagcgata aaatacgctt ccctgcttca tcggcatcaa ggccaaaggc cataattccc
    40681 tcctggtgac cagccatgac cagcactctg gttttacgga gatcggtttg tctgtatagc
    40741 cttttgattt ccatggccat ttggggagtc ccgtactcgg cattagacag cgttgtgggc
    40801 actttcccga taatccgctt ccaaagttca aggttgtgaa tgtggaacac cgccttaatt
    40861 gccttatcgc actggtaaag tgcggcatgg gtaagcgatt cggaagaagc aataaccggg
    40921 cctttggcct ccacccagtt acgcccaatg ctgaaatctg taaccagcgt gtagtgcatg
    40981 gtggtaagct cggtaagttg tcctgttcct gagcctgtaa tgacgaactg gttgtgaagg
    41041 taacgcttgc tcaggtttcc aaaaccaacc ccgttacaat ccactccaac cagacctttg
    41101 ttgtagagta tgcaacgcca acggttcaag tcggcaaccg cttcgtaggg aagagggtcg
    41161 gcgttaaccc agctgcacct gaatttaata atgcccttct cgttcaatag ttgaattttt
    41221 caggaaaaac tttctttatg ccagcaggta agggcttgca aattccccgt tcggtaataa
    41281 tcccggaaat taatcgggcg ggtgtaacat cgaaggcaaa gttggcggca gggctttcat
    41341 cagggcaaac cagcacgttt tgaagcctgc catccagcca gccaccaacg taccgcacct
    41401 catcgggatg gcgctgctcg atgggtatct gcgaaagccc gtcgctgagc gaccagtcga
    41461 aggtggatga aggcgctgca atgtaaaatg gaaccccgtt gtcgtgggca gcaagagctt
    41521 taaggtaggt accaattttg ttggcagcat cgccgttggt ggtgatacgg tcagcaccgg
    41581 ttaacaccaa atccaccaac ccatgctgca tgaggtgtcc tccagtgtta tcggctatca
    41641 ccgtgtgggt aattccctcg ttgagcagtt cccatgcagt aagcgatgca ccctggttgc
    41701 gtggccgggt ttcgtcaacc caaacatgca gccttacacc ccttcgccat gcctcgtaaa
    41761 cgggagccag ggctgatcca taatcggtaa aagccagcca tcctgcattg caatgggtta
    41821 gaatattgac cactccgcca ttcttgttgc gggcaatgtc ctcaattaaa cctactccct
    41881 ccagcccaat tttccgacag caaccagcat cgtcttcggc aatatctata gccgtttgac
    41941 gtgcaatggc caccctatcg tcgggattct caacccctgc aagaacttcc agctgccttt
    42001 caaccgccca tctcaggttt acggcagtag gacgtgtttt aatcagcttt aaggcacact
    42061 tctccaacgc ctcctgaaaa ttgacaaggt ttctttgctc ccacactgca aggtagatcc
    42121 caaaggctgc cgctacgcca attaagccgg ctcccctaag gtgcatttcg gaaattgccc
    42181 gtagtaaccc atcaaagctt tcaataggct caactacaaa ccgatggggc aggtatcgct
    42241 ggtcaattat tttaacgcat gggctgctgc catcctccag ccatatcgtc cgatagttaa
    42301 ctccgtttac cttcaactct ttctttcgct aatgggttaa ggagggctta tccactctca
    42361 taagcaccac caaatttaaa ctaatggcaa atttacccaa aaacgcgtta ataaattgga
    42421 atgaaattgc ttatcagaaa atcgtggggt tccttcatgg cgttggtata atgtatcatt
    42481 gccttaacat tcaaaatagc atgattcata aaaaattgaa cctcttttag gtcaaaaggt
    42541 tatattttaa aggtaatttt acatttttta aaaatctgct ggccatttgc agcaggtaaa
    42601 ttggatttaa aaaggtacaa aggctaagcc aacatgaaca agcgtatttt catactgata
    42661 attatagacc tgatgttgat actggtagct ttctacctat cggtactgct gaaaggggta
    42721 agttttgcca gctacctgga gagctaccag aatgcacttc tgctctttgc cctgatatgg
    42781 attgcaacgt caatagtttt cgagaagtat gtgtttacaa cccattcggc ccgaaaaata
    42841 tcagcaaata ttattaaatc aaacctcctg gcatttgccg ttgtaacttt tctcatatac
    42901 ttcacccggt ccgacaggta ctcccggttt atcgtgttca acaccattct gatggtaacc
    42961 ctgctggagc tattcatata caacctttgg gccggattaa agcgaatcag ggttatcccc
    43021 gataatttgc tggggatgat tgagcggagc gtaaagcgtc gtaccccgcc catccaaaca
    43081 accagggcac ccaaccccat gagggttgag ctgattaagc gaacgttaca agcagaattt
    43141 gggaacgagg tttacgagta cctaaatacc aacacccgat tatttgatga taaaacgctg
    43201 atagtgtcca ccactacccc attcaacatt ataaaccaac ccgatggcta tttcgacacc
    43261 ctaatcaacc tgaaacgaac aaacgacatt cgcaggatta acaagttttt tgaggcagtt
    43321 aaccagaagc tgccacgggg tggaatattc gtttgcatgg ccgaaacgca ggagcagcgc
    43381 aaaaagcgaa tactttcaaa gtttcccccg gtgataaatt ggatatacta cttcttcgac
    43441 tacgtagtta aaagggtttt cccgaagttc tcgctaacca agggtatcta cttttttctg
    43501 actcgaggtg aaaaccgcgt gattagcagg gcagaactgc tggggaggct atactcctgc
    43561 ggcttttacg taaataacga gaccgaaata gaaaagctac aatacgtagt ggccatcaag
    43621 aaccgaaagc cctttttcga cgaggaacca acctacggtc ccattatcaa gcttcgcagg
    43681 gtaggaaaga acggaaaaat tatcagggtt tataaattcc ggactatgca cccctactcc
    43741 gaatacctgc aggagtatat tttcaacctg cataacctgg aggagggcgg caagtttcga
    43801 aacgacttta gggtatcaac ccttggtgca gtaatgagaa gattatggat agatgagctg
    43861 cccatgatta caaacctttt tcgtggtgag cttaagatag ttggcgttcg accccttagc
    43921 gagcaatact tcaacctcta cacacccgag ctgcaggaac gccggataaa gtataaaccg
    43981 ggtttggttc caccgttcta catggacaat cccaaaacgc tggaggagat aatggcctcg
    44041 gaggtaaagt accttgatgc ctacgataaa catcctttgt ttaccgacat acgctaccta
    44101 ttcggggctg cctacaacat aattttcaaa aagtaccgca gcagctaggt gcttaaaacc
    44161 caaagcaaac
//
LOCUS       DAOPHX010000004        43469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_788_length_43469_cov_5.920302, whole genome shotgun sequence.
ACCESSION   DAOPHX010000004 DAOPHX010000000
VERSION     DAOPHX010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 43469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43469
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_788_length_43469_cov_5.920302"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..538
                     /locus_tag="PK502_00695"
     CDS             293..538
                     /locus_tag="PK502_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HPG99365.1"
                     /translation="MERRIGAALILVENRGSVEKLNQLFSAHSSVIVARQGIPLQQRG
                     VSVISLVLDGTSDEISALTGPVGKLDGVQVKTLMMKG"
     gene            770..2197
                     /gene="hydG"
                     /locus_tag="PK502_00700"
     CDS             770..2197
                     /gene="hydG"
                     /locus_tag="PK502_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPG99366.1"
                     /translation="MKFNPEVYSIPDERMKPFIDPDEIWGYINSAKPTRERVREIIAK
                     SLDKHRLNLEEVAVLVNTSEPELVEEILQGARTLKQKVYGNRIVLFAPLYIGNKCTNN
                     CKYCGFRTSNTEAVRVTLSDEEITKEVEALEDNGQKRLILVYGEHPEYSADYIAHTVR
                     LVYGVKKDRGEIRRVNINAAPLSVEDFLKVKKAGIGTYQIFQETYNPEAYSWYHQAGK
                     KRDFEWRLTSLDRAQEAGLDDVGIGALMGLYDWRFEVLGLVRHTNHLEACYNVGPHTI
                     SFPRIKSASGLDITNQYFVSDDDFVKLVAILRLAVPYTGMILTAREPVHVRQKVLQFG
                     VSQIDGGTKLELGAYIESKNEKQNLNREQFEIGDSRSLGEVVDDLVEHDYIPSFCTAC
                     YRLGRTGEHFMEFSVPGFIKRYCTPNAVLTLTEYLVDYATPEVKDKGYRLIDKTLTEL
                     DGNQNVEEIKRRMERIKAGERDLYF"
     gene            complement(2498..3922)
                     /gene="pyk"
                     /locus_tag="PK502_00705"
     CDS             complement(2498..3922)
                     /gene="pyk"
                     /locus_tag="PK502_00705"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPG99367.1"
                     /translation="MLKRTKIVATISDQRCEVSFIKSLYDAGMNVARLNTAHMNPSGA
                     KILIDNIRKVSDHIAILVDTKGPEIRTSSKGAEVKVQQGMTVKISGDPNGESGGDNIF
                     VSYPNIHKEVPVGNAVLIDDGEIELRVRSVSHDALVCEVENGGVIKLRKSVNLPGVHI
                     NLPSLTQKDKDFVQFAVENRVDFIAHSFVRNKHDVLEIKEILQQQKSPIKVIAKIENQ
                     QGVDNIDEILDHAYGIMVARGDLGIEIPSEKLPIIQNRIVRKCIESKKPVIIATQMLH
                     TMIEHPRPTRAEISDIANAIYQRADAIMLSGETAYGAYPVEAVNVMTRVANEVEDVLE
                     TDSSINLARINNEVTAALAKSAVRACSMLPVRAIIVDTLTGRTGRYLSAFRGQIPIYA
                     ICYNEHVMRQLALSYGIEAFYMEPRQSRDHFLSDAISYMLEQRKIASEDMVLIIGGSF
                     GPSNGATFMEIGKVKNLAKKKGEF"
     gene            4165..5061
                     /gene="xerD"
                     /locus_tag="PK502_00710"
     CDS             4165..5061
                     /gene="xerD"
                     /locus_tag="PK502_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HPG99368.1"
                     /translation="MGWSAAIEDYINFLKLEKSLSKNSVAAYHTDMMKLYGYAEIIGV
                     EPESVTGEQIQDFIAHLHDSGLSKRSQARIVSSVRGFYKYLLIEEVVDSDPTELIELP
                     KTGRKLPEVLSVEEIDAMEAAVDLSMPDGHRNKAIIETLYSCGLRVSELVNLRLTDLF
                     FDDGYVRVLGKGNKERLVPIGRKAIADINMYLSQRIMVSITVDAKSGNIVFLNRRGKQ
                     LTREMVFLIIKKYAKAAGITRNISPHTLRHSFATHLVEGGADLRAVQEMLGHESILTT
                     EIYTHLDKEFLRETILKFHPRG"
     gene            5093..5272
                     /locus_tag="PK502_00715"
     CDS             5093..5272
                     /locus_tag="PK502_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPG99369.1"
                     /translation="MDTGEKIVKTLSSAGKPMKAGEIAEAAGIDKKEVDKVIKKLVAD
                     GKLHSPQRCFYDIKK"
     gene            complement(5422..6189)
                     /locus_tag="PK502_00720"
     CDS             complement(5422..6189)
                     /locus_tag="PK502_00720"
                     /inference="COORDINATES: protein motif:HMM:NF007757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPG99370.1"
                     /translation="MKISIIQASPFWESIEENLKLFTAKIESTSTDTNLILLPEMFTT
                     GFTMKPRECDASEAERTLDWMLQTSMVRKASIVGSIIARKENKLVNRMAFVMPNGQVV
                     FYDKRHLFRMAGEHNQYSAGNQRVVVSYMGWRILLTICYDLRFPVWCRNRNDYDLMLN
                     VANFPDERSYAWNSLLVARAIENQCFVAACNRVGIDGSGANYSGDSQVVDPMGQIVAK
                     ANPNSESTIEASISLEQLHEIRASFPAHLDADRFEIL"
     gene            complement(6363..7217)
                     /locus_tag="PK502_00725"
     CDS             complement(6363..7217)
                     /locus_tag="PK502_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPG99371.1"
                     /translation="MNFPADLTERFFVHNGQLLPTTNFTDSMVEGETVVYEVVRVAGS
                     TPIFAIEHLLRLQASIKNSNLPALANNSAASSLRMLTEKNPVAEKNVRITYSVSTNHP
                     VRNLTVYYIPSRYPTRIEKQKGVVLECIDAERPNPTVKVENKSLRELTNEIIESHGCY
                     EVLLVNAQGEITEGSRSNVFFISRDRLFTAPETKVLSGITRQKVVQICHSLNVELVER
                     CIGIKELAFMDGCFLTGTSPGVLPVRQVGNVSMNASIPVINAISMEYERLVEKSKAEW
                     HSFTGSSK"
     gene            complement(7572..8717)
                     /locus_tag="PK502_00730"
     CDS             complement(7572..8717)
                     /locus_tag="PK502_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99372.1"
                     /translation="MKKIYKGVMVAISVIGLVAILLFIFWQLQPSKKLNIYILDKTVT
                     RSNRPEHRALVWVLNQDRIVGPDGKTYRVNEDYWGFFPIDLNQQLFDFKAIRLNEVDA
                     YAAAYDAAYYADCYGVYSFEWYKNDAQPIRSSKVYGGLNQNDYLLLKSMKDMGKLIIG
                     EYNMFSTPTNALMRSKAEELFNVSWTGWSGVFCRNLTPNEAGGPAPWMPKLYESQHLK
                     PWPSEGKGIILLSNDGLVDVLVDGEDLNSAFPVIISDNTGISRFGLISEIPFAGWFEF
                     VVPGSNAVTPSTFKINTTQQGLQKLRAIGLTNEFPAVIQAKSNEFTFYFAGDFTENKV
                     TMFTSKMMGGKFLNQLLEGKSENSQFFYKFYSPLMENILNSYINKTQ"
     gene            complement(9136..11142)
                     /locus_tag="PK502_00735"
     CDS             complement(9136..11142)
                     /locus_tag="PK502_00735"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPG99373.1"
                     /translation="MFDKPNKLANEVSPYLLQHSLNPVSWYPWGNEAFEVAKRENKLV
                     VISIGYSSCHWCHIMEKESFTDEQVARLMNEKYVSIKVDREERPDIDHIYMSAVQIIT
                     RRGGWPLNCIALPDGRPVYGGTYFPKDQWLNILESMDATWKSEPERVYEVAEDLAEGI
                     ANTEIIHVKTKLTELDFTGIVMGNLQSIEPALDYGLGGTRGAPKFPMPGLLGYLMQSA
                     QATGNLKLKSFVSTTLDNIANGGVYDHVGGGFFRYAVDSRWHTPHFEKMLYDNAQLVE
                     LYSLAYRINPNPSYLNIVFNTVDFLDRELHSPDGGFYCSLDADANGLEGGFYTWTKQE
                     LDEVLEFDSEIFCSAYGVTPSGDINGRNGLKRCASDDQLSSLFDIPPQEIQFRLQRSL
                     KTLASIRNKRIPPLIDDKVLASWNGLLVSALAQAFITFKNPRFIGLASEIASYLETKH
                     FVEGNLKRVFCKGKTYGNALLDDYANYIRALIMLYQATLEEKWLFKANSLAQEAIRDF
                     WDEGKGMFYYTPGSSDLMVRKMELIDGVIPSSSSIMTGNLLFLAKHIENPLYSKIAMQ
                     VLANLAENTKNNGVFLYGWGNLMLSLALPEVSITIFEGTSNDVNDILGRVIYPNIYFE
                     LIAKKVDHKFHLCVGSSCRLPMDSAADVAQAVNGIGLHNNSSNF"
     gene            11321..12733
                     /locus_tag="PK502_00740"
     CDS             11321..12733
                     /locus_tag="PK502_00740"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPG99374.1"
                     /translation="MKNLLFALSFSFLSFGVFAQNHNGYRIEIKIDGLKDTTLLLGYH
                     FGDKKFVSDTARIDSNGVAIFEGDSLLKGGIYLVILPDKTYFEVLLSNNQRFGVQTTA
                     KNMVENLKFTGSKENEEFIHFQQFMMGVQAQSNELSAKMKDALTDSTRSNQLRKEMNE
                     LDEKVKAYWLEVENNNRGTFLASIIKAMRSVEVPEFTIPANTPAYDSLKWVHSYNFNR
                     DHYFDNIDFSDSRLLRTPILENKINIYFDRVLIPHPDSIAAAAVNLVEKSKANREMLQ
                     YILSYLINKFQTSERMGMDGVFVVLAEKYYLGGQAWWADQKLLDKISERVNALKPNLI
                     GKVAPDLWLPDPNGKYFRLSNINSKITVIFFWDPECSHCKKEMPKLKSVYDNYKKYGL
                     EVYAVYTQGDQPKWMESISKNDLTWINVWDPTFSSNFRNLYDIYSTPVIYVLDSGKKI
                     IAKRISVETLESILKQELKL"
     gene            13017..14495
                     /locus_tag="PK502_00745"
     CDS             13017..14495
                     /locus_tag="PK502_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPG99375.1"
                     /translation="MESLLTNTYFWVVLAYFAVVLFISFFTKKLASRSSADFLVAGRN
                     LGLLVCSMVVAAEWLGGLSTVGVSEKAFDTLTLQPILYNFSTAIGMVILGFTLAKAYR
                     ERKVNTVSEMVESIFGKRARSISAIAFLIAFITLAYVELTTFATVLSPIFNINWVWAV
                     IIAATITTIYTYMGGMHALTIVAVFNLVVRYVGLGIALIIGLVAIGDFSNLTNRLVEI
                     GAPQNLYNPFSIGWNAALSLILGGIFGGMASQASIQPIFAAKNPITAKRAAILSAVFI
                     APFGIITGILGLIARSGLFFEVTPGFNPKMALPRLLMTPEFVHPVIGGIAIAAILAAI
                     LSTIGPVSFSIVTIATKDIYHGLINKKASDEKILQFAKRLVILVNLVTVPLALFMKGA
                     ILDAGYFSYAIRTIGAIVILLGIYKLKWISTLSVQLSFIGGTLAIIISIVAKKFGWFD
                     IDKTYASIIATVFFIIIGNIYTKYFTGKQKSSELNINESATK"
     gene            14476..15687
                     /locus_tag="PK502_00750"
     CDS             14476..15687
                     /locus_tag="PK502_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HPG99376.1"
                     /translation="MNQLLNDIRVLELANVLAGPSVGMALAELGARVVKVENLLTLGD
                     VTRTWKLPSEDPDTNISGYFSCVNWGKKSLAIDLNKPEGLDIVYRLTEVSDIVLVSYK
                     PGDAEKLKVDYNTLKQYNKSLIYAHLTGYGLKNERAGYDAIIQAESGFTYMNGEQGGK
                     PVKMPVALMDILAGHQLKEAILLALLNRFRKGEGTYIEASLIKSGVAALANQATNWLV
                     GNSIPQRMGSDHPNIVPYGRIFYTRDEKPVVLAVGSDKQFAELCSILGKPELAQDPKY
                     STNFNRVKNRNEIYPILQDLVAQIDRDPFLNELARRRIPAGGVYNMQEVFDQPEAQEL
                     VMSGTTSAGTPIKGLRTVAFSYNENSKPLELAAPPYYGEHTIQILKETLGFSNDCIEG
                     LINKNVVYAPR"
     gene            15674..16798
                     /locus_tag="PK502_00755"
     CDS             15674..16798
                     /locus_tag="PK502_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPG99377.1"
                     /translation="MHPGKTSLAFIDGSRLITESLVRAGADTFIGYPITPANLLYLYA
                     TKRYPTMLAAPDEITTLQWMSGFAIAGHVPVTATSFPGYALMLESINMAAMFELPMVI
                     VLVQRLGPATGTATCGAQGDLSLVHGTLSGGYTLPTFSPSNLNDCWDISAKALHTAIR
                     LRTPVVLLTSKEMVMTLQAFDWSHLAEIETLKVKTFSGSDTYIPYKVDESLVPDFMPV
                     SQNNHAVRLTASTHDTKGFLQNSTPEAIENTKRLEAKVTKHLQEYLLFDLDEQAGAQH
                     LVVSYGISAQASREAVRQLRARGEKVSLLVAKTLLPVSRDYVDIANRYERVVVVEENL
                     TGQLRQLLFGAAGRNGLSGVNAIARMISPSEIVDEVMRKF"
     gene            16886..18154
                     /locus_tag="PK502_00760"
     CDS             16886..18154
                     /locus_tag="PK502_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPG99378.1"
                     /translation="MNNVKFLTGGQLPFCKGCGHVAVAQNTEKALQKLDFNPLDVVLV
                     TDIGCHGIVDKNFLTHNVHGLHGRSSALAAGISAGLSNPNKKVIVFTGDGGATIGMQH
                     LVGGAHLGFDMTVVVHNNMLYGMTGGQPSEFTPCGFKTPTLPEGSTKSGYDICELMLA
                     AGAAYVERVVGIGDFSDSLARAFSTKGFSLVEVMEICTSYGVKSNPGMKLPKLVEEAG
                     WKVKVFTEAKQRLFQTPQNSNPTSLLSEKLEVEPKYSGAISKPVSIMLSGSAGEGVQL
                     AAEFLARAAMLSGLYATKKGSYPVTVGVGYSAAEVIISHEPILYTGSPVPDILAITSA
                     DGLGYARTAAGRMKGGTIYIDQSLDIPQTGAETVVIPFREKVGAQNSSLYSAFYMVGS
                     QHFFPMEALRDIFMANKISQRVSVDIFMQL"
     gene            complement(18731..19405)
                     /locus_tag="PK502_00765"
     CDS             complement(18731..19405)
                     /locus_tag="PK502_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HPG99379.1"
                     /translation="MGIYLIMIIFFVLSFLISNQLKVRFARYSKIDLPQGMTGKEVAE
                     MMLKQHGIFDVRVISTTGQLTDHYNPTDRTINLSPAVYNGHNIAAAAVAAHECGHAVQ
                     HAQSYAFLQLRSSLVPVVSVASRWVQWILLAGVILLQSMPAILVVGVALLALTTLFSL
                     ITLPVEFDASRRALQWLGSTGILYGQNVAYARDALKWAALTYVIAALGSLATLFYYLG
                     FLNNND"
     gene            complement(19452..20540)
                     /locus_tag="PK502_00770"
     CDS             complement(19452..20540)
                     /locus_tag="PK502_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPG99380.1"
                     /translation="MIDYLKLKSYISLLFERMGCSEMDAKSVADLLVAAELRGIPSHG
                     VMRVKDYYQMVAAGRINTRPSVKTIHETPSTATVDGDNALGPVAGLYSMRLAIEKAKV
                     AGTGWVATQNSNHYGIAGYYAMMALEQDMIGISMTNANPLVVPTFSTSKLLGTNPIAV
                     AIPALEQPPFVADFATAPIARGKLALMAKKGEKAPIGLVQDGLGKSTDDPDTLLHGGG
                     ILPLGGDYEHGSHKGYCLTSIVDIFSAVLSGANFGPFVPPQVPYLPIPEHSVGKGLGH
                     FFGAIRIDAFRPANEFKLMMDEWIKTFRAAKSGVNQPPVLIPGDPERENERIKSKEGI
                     TILPQVLNDLNEVAEKLAVEPLSVFSSK"
     gene            20968..22251
                     /locus_tag="PK502_00775"
     CDS             20968..22251
                     /locus_tag="PK502_00775"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPG99381.1"
                     /translation="MTYNEALDYIVSQLPMFHRVGKAAYRADLGNALVLDEFFGHPHQ
                     KFASIHIAGTNGKGSTSHMLASVLQQAGYKVGLYTSPHLKDFRERIRINGEMISQVEV
                     TRFVEEYRALFDRVKPSFFEMTVAMAFDYFARRNVEVAVVEVGLGGRLDSTNIITPIL
                     SVITSIGLDHTDLLGSTLPQIAGEKAGIIKRGIPVVVSQLQPEIADVFTSKAGEMQSP
                     LVFADSEYKVREMSYSASGTQTFLVDHGGNPEQWDTDLMGSYQRLNLPGVLAALDVLR
                     NEGYRITSDAQKQGLRMVQQLTGLMGRWQKLQDRPLIYCDTGHNVDGITMVMEQIHRM
                     SYAKLHVVLGMVADKDIDGMLSILPADAIYYFTRAALPRALDQNILKTKAKGFGLKGE
                     AYPTVAQALEAAKGSANEDDLIYVGGSTFVVAEVV"
     gene            22344..22418
                     /locus_tag="PK502_00780"
     tRNA            22344..22418
                     /locus_tag="PK502_00780"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22379..22381,aa:Val,seq:tac)
     gene            complement(22501..23790)
                     /gene="murF"
                     /locus_tag="PK502_00785"
     CDS             complement(22501..23790)
                     /gene="murF"
                     /locus_tag="PK502_00785"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPG99382.1"
                     /translation="MLEELYHHYLNHGSVATDSRSIPKGAIFFGLKGDKFDGNKFAEQ
                     ALNNGASLAVIDNPTFMTHDCILVENTLKTLQELANHHRKQLKAKIIGITGTNGKTTT
                     KELIQCVLSKKYHAVATKGNLNNQIGVPLTLLSLTADVEVAIVEMGASHPGDIAELAS
                     IAAPDIGIITNIGKAHLEGFGSYEGVVRTKGELYDYIARHKGLIIYNQHNEVLKQLVM
                     GIGIANKLEYSNYCKSIKVTNQHDTPFISVNVEMAGTDKPMDIKTKLVGSYNIENIEA
                     AIAVGLHFGVKPELIKNAIEEYNPSNSRSQLIQTKRNTVIMDAYNANPTSMEAAVRNF
                     TSLESPNKIIILGEMLELGSYAESEHKKVVKLLESLGATQVFLIGQNFSGINSSYGWF
                     ADAKACAEYFLSHEVANATILLKGSRGVGLEHVLDVL"
     gene            complement(24036..25547)
                     /gene="gldJ"
                     /locus_tag="PK502_00790"
     CDS             complement(24036..25547)
                     /gene="gldJ"
                     /locus_tag="PK502_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldJ"
                     /protein_id="HPG99383.1"
                     /translation="MNLKNKPLSLILVAMLGLTSCGISGKGGSNVSSKTGWAYNSPDE
                     GGFEVVTDWKPEAGPGLVFIEGGTYIMGRVEQDLMYDWNAKPRRVTVTSFYMDETEVS
                     NVDWREYIYWLKRVYSDYPQVIKQALPDTLVWRSPLGYNEPFVTDYFRHPAYNDYPVV
                     GVNWLQANDYCQWRTDRVNEMLLVQKGILEIELGQKGDDVFNTEAYLAGQYVGKVKEN
                     LPSLNPEQPEGRPVRFEDGILLPKYRLPTEAEWEFAASGLVGNTYEERMVERRIYPWN
                     GHNVRNSESKNRGQMMANFVRGRGDYMGLAGNLNDQNTFPGPVKSYWPNDYGLYNMAG
                     NVNEWVLDVYRPLSYEDFDEIRPFRGNVFKTPYDKEGNFAKKDSLGRIPYREVTEEEA
                     ANRRNYNKAYYINYKDGDVASTLNYVEGETLKTKDSNAMYDQGKPDAKGAGMTSLIND
                     HVRVYKGGSWKDRAYWLSPATRRYLDEASSTNDIGFRCAMEAVGPQSARQRGE"
     gene            complement(25627..26607)
                     /locus_tag="PK502_00795"
     CDS             complement(25627..26607)
                     /locus_tag="PK502_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPG99384.1"
                     /translation="MFRKFFVAIGITMVTSIRVFGQDPIFSQLFFNPLYLNPAYAGAS
                     KYMRLGVVYRNQWTMIDMPYSTYGVSFDRTIQSIKSGVGLNIVADVEGKGVFSRTTVD
                     LVYAYGIQPSYNSHLRFGIQTSGIVRTRNYSGLVFPDMIDINGDVTGTYNTSGITTWN
                     YDFSLGVAGDFENFYGGIAVHHLLEPVEATYQDTKAYIPRKYTVHIGADFNLYKWYKF
                     KEVLLLSPNIIYIQQAKFNQLNIGFYLSRHWLVGGFWLRENLSLNSHSFIFTIGYTDD
                     LFRMGYSYDFSIMKYGLRGLPTSSHEVTLGWNFEYKKGKSKYKYIKCPKF"
     gene            26741..30547
                     /gene="porU"
                     /locus_tag="PK502_00800"
     CDS             26741..30547
                     /gene="porU"
                     /locus_tag="PK502_00800"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HPG99385.1"
                     /translation="MFRRTFFLSLLCISNGIFAQDTLERAICWNYRQVPTAQAEQTIL
                     WFDNAVYTNPQTMIPSYFELIPIENNGAYNSTPDVLVADKQWVLLANDEMNLLQGVNN
                     FINESVDCRTAIQRGRMMIQLTVPAVRLNSDNKFEKLVSFKIILKRAEKTNDERSYAP
                     ARLQVKSSVLSSGSWVRVSVREGGVYKISYNDLQSYGLSNLENVSLWGNGGHCLPYMN
                     SEQAPDDLNPIPILMSKGDDGIFNQGDYILFYAEGPKTFSYNATMDMWEEKSHPYAQQ
                     ISYFITTSLPQSTIETIEESAAPLNRTANSYDAFLTIERNDTNMVKSGREWFGDVFDL
                     TTSRTYTTDLSLPVVGLPLKVWLRAAARSSVASNFTVKVNTTTLGTLPLNSVVIGDEY
                     ADVVAVNEQVYTSALNEGNLTIELTYSKPNSTATGLLDFITINTRQQLKYNGSQLIFR
                     DKQSVGSGNITEFSILNSNQNVQVWDITSFNHPYRLNSRYSGGVVAFKQATDTLRHFI
                     AFEPEKAKTVTFVGAVANQNIHGAGQPDFVIVAHPLFMPQAQELAQLHRSLDGLDVVV
                     FSTDEVYNEFSSGNPDLSAIRNMMRYFYRCAATTEQQPKYLLLFGDGSYDNLSDRNGN
                     TNYILTYQSPRSINKVGSFQSDDYFGLLDDEEGEAVGLLDVGVGRLPVSTTEQANAVL
                     AKIQHYVSGSNTGDWQSKLCFIGDDEDGNIHMTDANTLADYLSTSNPEYNIQKIFFDA
                     YRQESSSGGASYPEVTNAINTTVNNGVFLVNYTGHGNERWLAHEKVLMLSDVSSWKNT
                     KQLPLFVTATCEFSRFDDYHLTSTGEQILLSPDGGGIALVSTTRLVYSSPNFTLNYKF
                     INNLFKFNEDSTSHYRLGDLIKISKNLSGTGNNKRNFTLLGDPALMLRYPELGAEITS
                     INGKPVDEEADTLKALSVANLSGRIFTPIGQVASDFSGNATITVFDKKSEIKTLANDG
                     GMPMTFFSWDNVLYKGRAIVNNGLFSIDFVVPKDIRLSFGTGRISIFADDLARVAMGY
                     NESVIVGGVFNSPYTDNEGPEIDVYLNDTLFNGGGISDSSPKLLIRLRDESGINTSGI
                     GIGHDLTATLTSPGGDEVNVILNMYYQSEPNSYQEGYVNYQLADLSEGRYWLRVKAWD
                     TYNNSSEKIISFVVVGAGNLQLEKFYSYPNPFSEGTSFYFEHNMPEDEFNLTIQIFDV
                     AGRLVSTITQSQAYSGGYRVGPIYWNGTDSRGSRLGRGIYICRLSVKTSSGYAASLQH
                     KLVILR"
     gene            30623..31840
                     /gene="porV"
                     /locus_tag="PK502_00805"
     CDS             30623..31840
                     /gene="porV"
                     /locus_tag="PK502_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HPG99386.1"
                     /translation="MRRILFTLLSFVGFIFFSQVSSAQFDSTVSLTGGLNALKIAVPF
                     LTIAPDSRAGGMGDAGVATSPDINSQHWNPAKFPFIESKKWGVAFSYTPWLRNLVNDI
                     NLTYLTGYYQVDKMQAISSSLLYFAMGEIQFTTDNPNDPPVKFFNPNEFAIDIAYSRK
                     FSDYISGAIAFRYIRSDLSGGYSQPGQASAKAGTSFAADIAMYYQHPVKLSDKFGQMA
                     FGFNISNIGTKLSYSDDPMKDFIPINLRLGGRLTMDIDSYNSFSFTVDANKLLVPTPP
                     VYGVVDEQTVIVKGMNPNVSVVQGMLQSFYDAPGGFKEELNEIYYATGIEYWYARQFA
                     LRAGYFYENENKGNRKYFTVGAGLKYNIFNIDFSYLIPTGGFNSPMANTVRFSLSFDF
                     EATRMRGQKVNNN"
     gene            31844..32323
                     /gene="ispF"
                     /locus_tag="PK502_00810"
     CDS             31844..32323
                     /gene="ispF"
                     /locus_tag="PK502_00810"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPG99387.1"
                     /translation="MKVRVGFGYDVHKLESNTPFHLGGIFIEHSKGAVGHSDADVLIH
                     AICDAMLGAANLRDIGYHFSDTNPNLKGIDSKILLAETLKLLDSKGFRVVNIDSVVCL
                     QRPKLMDIIPNMQLTLASVLKMDMEDISIKATTTEKLGFVGNEEGVTAYAVVLIQKD"
     gene            32329..32718
                     /locus_tag="PK502_00815"
     CDS             32329..32718
                     /locus_tag="PK502_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPG99388.1"
                     /translation="MVPENAETFSTLVLGASCKAGRFSYLAIKSLVKHGVRVYAIGSR
                     PCFVDGVQVHAEKINLTNLHTVTLYLNSQNQTTYYDYILSLKPKRIIFNPGAENPELV
                     RLAHENRIDYVFGCTLVMLNDGSFFKS"
     gene            32965..33558
                     /locus_tag="PK502_00820"
     CDS             32965..33558
                     /locus_tag="PK502_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99389.1"
                     /translation="MKKTVLLGFAALSMVAVLSGCGKVPQEKIDAANAAIDSAKMVEA
                     DVYVAADFAAVQDSMSAVMADIEVQKSKLFKKFGPANEKLDRILSQAHQIQANAVTKK
                     QEVKVEVEGLMTEVTTLIDQDKTLMKKAPRGKEGAAVLEQIKTEIAAIEASVAEAKTM
                     YDNGKYMDASNKLKAAKDSATKINTELTDAIAKVKKR"
     gene            33845..34990
                     /locus_tag="PK502_00825"
     CDS             33845..34990
                     /locus_tag="PK502_00825"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPG99390.1"
                     /translation="MSRKSKRIAVIILASLVVATGISVLFYKLIPEPPIGDVDYARLC
                     LQQASKSKAQSYSKKSFKQAKADYDSAMKCWQKENEKFILFRDYSMVAKFAQKSAKEA
                     RNAMNNSQNLRKNMVVAVKDKIELLNGLILNVNELFNRVPLPQEVRDKISRGKLLLKE
                     AQLYFERGEYLSAQDKIVLAEDMLMSSYNRTLDDLEEYFNSQPYWRKLVNSTIENSAK
                     TNGNAIIIDKFARKLYIYQSGVKKLEFEAEFGKNWMGRKRLKGDKATPEGIYLVKTKI
                     PQNKTKYYRALLLNYPNNDDVEMFRREKQQGKLPANAQIGGLIEIHGNGGKGTDWTDG
                     CIALSDNDMLKVYNFIQVGTPVVIVGSLADFKSITENFKLRKHTERS"
     gene            34997..35722
                     /locus_tag="PK502_00830"
     CDS             34997..35722
                     /locus_tag="PK502_00830"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPG99391.1"
                     /translation="MDAVNIALATTDKDKSIGKVSKFNNILAGLLIVILSIILFFAIL
                     IYAIPSLTELVMKVNGFAPPSSEIFSPTPKDYAKIKLLTGKMQQKYVALTPPRPYIVV
                     NTTYNRFKLYKNRRLIREGFCSTGSYTLLKSHFNREWIFQTPKGFYRIQGKITNPVWR
                     RPDWSFVEEGLPIPSQNDPSRWEAGVLGDYAMSIGDGYLIHGTIYKRFLGMPVTHGCI
                     RMNDDDLEAVYKTLELGSKVYIY"
     gene            35734..36519
                     /locus_tag="PK502_00835"
     CDS             35734..36519
                     /locus_tag="PK502_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99392.1"
                     /translation="MQKKFLKIFLLACLPIFALALVYFILKLVAVNGAVDRFQKFYSQ
                     LDGDDKSNAVFQLTNDSAYNAEQNSYAYYHAQLEAANSDSVVLCINFPDSTATLLIKG
                     VTVHTAKIDRYRVSNFFTKIDQRILYKALSYPFRVERQTSSIPKVPLMIKIAPKDTSE
                     YTPDIVPDTTDVNPVYFLLEAKPGMLVYFYETPKNQSRISFNGVITDIKDRTAILLDD
                     LQRIVHFRKLDYKPSITLRLNRKDAKIIFRAIPNDALIILKLS"
     gene            complement(36648..36725)
                     /locus_tag="PK502_00840"
     tRNA            complement(36648..36725)
                     /locus_tag="PK502_00840"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36688..36690),aa:Pro,seq:cgg)
     gene            36910..38190
                     /locus_tag="PK502_00845"
     CDS             36910..38190
                     /locus_tag="PK502_00845"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HPG99393.1"
                     /translation="MVESFIHFLWQYKSFAFSGLNTSDNCQLEVLDPGTYNSDAGPDF
                     FNAKVRLNGTTWVGNVEMHINSSEWFAHNHHTDRAYDNVVLHVVVNNDKPAINSRGVP
                     IPTLAISYPFGLEREYSRLTSSGSYIPCGEVLPNVDNLTKRALLDRMMVERLQAKTDE
                     IGKSLEKCHGSWEEAFYRAVTRTFGLKVNAFPAEILAQETPLKVLAKHKNNLFQLESL
                     IFGQAGLLNRFDVEDEYLLSLRMEYSFLKKKFNLTPIDGSLWKFLRMRPVSFPTIRLA
                     QLAMLIHHSTSLFSKAVEASSLKQLQELFQVETSPYWYNHYLFGFPVKSRKKSLGTAT
                     INVIIINAVIPFMFAYGGWRGNQKLKEKAISILEEIPPEENHIIKKFDSMGVKSCNSS
                     DSQAVIHLKSTFCDARKCIFCPVGIKLLLKAAYI"
     gene            38296..39198
                     /locus_tag="PK502_00850"
     CDS             38296..39198
                     /locus_tag="PK502_00850"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPG99394.1"
                     /translation="MRILVTGANGLVGKKITRQLLSGGKHEVYATSLKKLQIPGAVTF
                     TSNLLNADINFMVDETHPDVIIHCAALSSPDACEVDRFACKRLNVDLTARIAMACNDY
                     GVQLILMSTDFVFDGLKGQYAEEDRANPICYYGMSKAEAEQVVASMSAPWAIARTSLV
                     FGYEQRLSRSNIVLRVAENLIKGNPFRVAFDQIRTPTLAEDLARGIEAIATNRATGIF
                     HLAGSEIISVADLAARTAEVFGLNPELLLPVSTEMLTEPARRPLNTSLCCHKAIEQLG
                     YSPKGLTEALRLVYSQMKLDGYNP"
     gene            39606..40868
                     /locus_tag="PK502_00855"
     CDS             39606..40868
                     /locus_tag="PK502_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HPG99395.1"
                     /translation="MKKISVNIQSEIGELEGVIIHTPGPEVENMTPENAERALYSDIL
                     NLSVARKEHAQLSGVLSKVTQTFEVGDLLEQVLSNPTAKEELIRRICLTENALWLMDD
                     LLAVSPKELGKFLIEGVPLVRNNLTNFLSHERFSLKPLYNLYFTRDASFAISRSVVIA
                     KMASTVRHRESIIMEAIFSMSDIFDTSTVNPSAFTLASNATIEGGDVLVVRDDVLLIG
                     NGARTNTHGIDFILNRLQLNSDARKKYILVQELPYKPESFIHLDMVFTLLDADKCMVY
                     DPIIIQPNRYQTVQITIANGRVEEIKTVDNLLVALKKLGMDLKPVYCGGTTDRWVQER
                     EQWHSGANFVAIGPGKVIGYARNVHTIEEMNKNGFEVIRAKDVIAGRVDLGSYSSYVV
                     TIDGSELPRGGGGARCMTMPVRRKKVNW"
     gene            complement(40877..42076)
                     /gene="sucC"
                     /locus_tag="PK502_00860"
     CDS             complement(40877..42076)
                     /gene="sucC"
                     /locus_tag="PK502_00860"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HPG99396.1"
                     /translation="MNLHEYQSKALLKKSGVNVPLGSVAFTPDEAVKVAESVIEATGT
                     DVIAVKAQIHAGGRGKGGGVKIAKSTEEVRRYAHDIIGMRLVTHQTGPKGKLVGKVLV
                     EQGIYQPGPAEVKEFYISILLDRSTSRNMVVYSPQGGMDIEAVASQSPQLIFKEEINP
                     LGLQPYQARKIAFNLGLTGKAYSQMVKFLANLYNTYEANDFSLLEINPVVKTSDDDIY
                     AADAKCTVDDSALFRHPDIAGQRDLDEEEPAEVEAGKNNLNFVKLDGNVGCMVNGAGL
                     AMATMDIIKLSGGEPANFLDVGGSANAKTVAAGFRIILKDPNVRVILINIFGGIVRCD
                     RVAQGVVEAYREIGNIPVPVIVRLQGTNALEGRKLIDQSGLRVYTATSFQEAANLVKE
                     MVEKQAF"
     gene            42152..42694
                     /locus_tag="PK502_00865"
     CDS             42152..42694
                     /locus_tag="PK502_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPG99397.1"
                     /translation="MAKNRKLSNEELHRKTVDEYKMVEKFPIVVVLDNVRSLNNIGSV
                     FRTSDALMVERVALCGICGKPPHPEIHRTALGAENSVLWEYFDKTEMAIEKLRGEGYR
                     IISFEQTEHSVNLVDYMPMPGVKYALVFGNELRGVGQDIVDLSDECVEIPQFGTKHSF
                     NISITAAIVLWDFFAKLKKH"
     gene            complement(42778..>43469)
                     /locus_tag="PK502_00870"
     CDS             complement(42778..>43469)
                     /locus_tag="PK502_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99398.1"
                     /translation="DKVGYSDDTLKYMVENNAYDSLNVEEMLYAATLVDNVDKKIEVY
                     MKAGEKFNCARGYNNAAYYYILNKNTAAAKAALDKIPADQKIPAVTNNRGCIALMEGN
                     YEAAEKLFADAADAGNEVKYNLGIIAILKGQYPAAAEYLGGTDSFNQGLALLLSGKVD
                     AAKNTFAKVETAKGYYGRAIVGARMQEEATTLDNLRTAIGKDASLKARAKDDVEFRNF
                     FQNEAFKAIAE"
BASE COUNT        11947 a         9310 c         9682 g        12530 t
ORIGIN      
        1 gatgatgcaa agttgttttt ccaaaaacag cttgccgatc atgttgacat agctgatgag
       61 gaacgcctga ctagtcagct ttcggcagct tgcagctcct tggatgagct cattttttaa
      121 ggagcagctc cgataaattt taaagctttt tgtgctgaag catagcgtaa ctatttgtaa
      181 ctttacgacg taattaaact tgtatttcaa ggttaaaaat ctgtatccgg gaaaggaatt
      241 ttaattgctt tcgaggattc agctaacagt tctaatccaa aggagaactc tgatggaaag
      301 gagaatcggg gcggcgctta tcctggtgga gaaccgggga agcgtggaga agttaaacca
      361 gctattttcg gcccattcca gcgtgattgt cgcccggcag gggattccgc ttcagcagcg
      421 gggtgttagt gttatctcgt tggtgctcga tggcaccagt gatgaaatta gcgcactaac
      481 cggtccggta ggcaagctcg atggcgtaca ggtaaaaacc ctcatgatga aagggtaatt
      541 ctacctattt tctctccttt acattagaat tgcgctattc taacctatca ttaggttgga
      601 gattatgggt atgcaaagca tattcattcc catgaagtat atatgttatg gggaagattt
      661 agtacgaagc gtgaaaaaaa tgatgaatta cttaaaatta tgaaatgagg ggcatagcaa
      721 ctctttgcat gcattaccaa ctaaatatta acaattaatt tgcaaacaaa tgaaatttaa
      781 tcctgaagtt tacagcattc ccgatgagcg aatgaaacct ttcatcgatc ccgatgaaat
      841 ttggggttac atcaacagcg ccaagcccac ccgcgaaagg gtgagggaaa tcattgccaa
      901 atcgctcgac aagcatcggc taaaccttga ggaggttgca gttctggtaa atacctccga
      961 gcctgagctt gttgaggaaa tcctgcaggg agctcgtaca cttaaacaaa aggtttacgg
     1021 caatagaatt gtcctatttg cgccgcttta catcgggaat aagtgcacaa acaactgtaa
     1081 gtattgcggg ttcagaacct ccaacaccga agctgtgagg gtaacccttt cggatgagga
     1141 aattaccaag gaggttgagg cgcttgagga taacggtcag aaaaggctga tactggttta
     1201 cggagaacac ccggagtact ccgccgacta cattgcccac accgttcgcc tggtttacgg
     1261 ggttaaaaag gatagaggtg aaattcggcg ggtaaacatt aatgctgcac cgctaagcgt
     1321 tgaagatttt ttgaaagtga aaaaggcagg gattggcacc taccagattt ttcaggaaac
     1381 atacaaccct gaagcttata gttggtacca ccaggcaggt aaaaagcggg attttgaatg
     1441 gcggctcacc tcgctcgata gggcacagga ggctggtttg gacgatgttg gaattggtgc
     1501 attgatgggc ctgtatgact ggcgttttga ggtgttgggg ctggttaggc ataccaacca
     1561 ccttgaggct tgttacaacg ttggccccca caccatatct tttccgcgta taaagtcggc
     1621 ttcaggtctg gatattacca accagtattt tgtttctgac gatgactttg tcaagttggt
     1681 ggcaatcctg cggctagctg tcccctacac cggcatgatt cttaccgctc gtgaacccgt
     1741 gcatgttagg caaaaggttt tgcagtttgg cgtctctcaa attgatggag gaactaagct
     1801 ggagctgggc gcttatattg agtctaaaaa cgagaagcaa aatcttaatc gtgagcagtt
     1861 tgaaattggc gacagccgat ctttgggcga ggtggttgac gatttggtgg aacatgatta
     1921 tatcccctcg ttttgcacgg catgttaccg cctggggcga accggcgagc attttatgga
     1981 gttttctgta ccagggttta tcaaacgtta ctgtacccca aatgcagttc tcaccctaac
     2041 cgagtatctg gttgactatg ctacacccga ggttaaggat aaggggtaca ggttgattga
     2101 taaaaccttg accgagctgg atggaaatca gaatgtagag gaaataaaac gtcgtatgga
     2161 gcgaataaag gcaggggaga gggatttata cttttagcaa aggttccttc cgtttttact
     2221 agaattatgt gatgcacagc ttataacctt gtatagccca tctaccttaa tagcagatgg
     2281 gtttttttat gtaaagtgtt aaccatagct gtcagctaat cccattcatt ttgcgcgtag
     2341 caagtgcggt tttaggaacc tgccatctgt ccacgacgcg aatcggagta taacatcaac
     2401 ccatctaaaa ataagcaaga tatgtagaac tctggaaaaa ttgtagagaa ttaatctgaa
     2461 aggcttaaaa atggttgaat tggctattta aacgttatta gaactcaccc tttttttttg
     2521 ccagattttt caccttgcca atttccataa aggttgcgcc attgctcggg ccaaagctgc
     2581 ccccaataat aagcaccatg tcctccgagg caattttacg ctgttcaagc atgtagctta
     2641 ttgcgtcgct gaggaaatgg tcgcgtgatt gacgcggttc catgtagaat gcctcaattc
     2701 cgtatgaaag tgcaagttgg cgcataacat gctcgttgta gcaaattgca taaataggaa
     2761 tttggcctct gaatgccgat aggtaacgtc cggtgcgccc ggtaagcgtg tccacaataa
     2821 ttgccctaac gggcagcatt gaacatgccc taaccgctga ttttgcaaga gctgcagtaa
     2881 cttcattgtt aattctagcc agattaattg acgagtcggt ctccaaaaca tcttcaactt
     2941 catttgccac tcgggtcatc acgttaacag cctcaacagg ataggcccca taagccgttt
     3001 caccgctaag cataatggca tcagcccgtt ggtatatggc gttggctata tcgctaattt
     3061 cggcacgggt gggacgggga tgctcaatca tggtgtgtag catttgggtg gcgatgataa
     3121 ccggcttttt gctttcaatg cattttcgta caatccggtt ctggataatg ggtagttttt
     3181 cggaaggtat ctcaatgcct aaatcgcccc gcgctaccat tatgccgtag gcatggtcga
     3241 gtatttcgtc aatattatca actccttgct gattttcaat cttggctata acttttattg
     3301 ggcttttttg ttgttgaagt atttccttta tttcaagcac atcgtgctta ttcctcacaa
     3361 agctgtgggc aataaaatca accctatttt caacggcaaa ctgcacaaaa tccttatcct
     3421 tttgggtaag cgagggaagg ttgatatgca caccaggaag gtttacgctt ttccgtagct
     3481 ttattacacc tccattttca acttcgcata ccagggcatc gtggctcaca ctccttaccc
     3541 taagttcaat ttctccatca tcaatcaaca ctgcgtttcc aacaggtacc tccttgtgta
     3601 tgtttgggta gctaacaaaa atgttgtcgc cacccgattc tccgttcggg tcgcccgata
     3661 tctttaccgt cataccttgc tgaaccttaa cctcagctcc cttagatgaa gtccggattt
     3721 ctggaccctt ggtgtcaacc agaatggcaa tatggtcgga tacttttcgg atgttatcta
     3781 tcaatatttt agccccgctg ggattcatgt gggcggtgtt aagcctggca acgttcattc
     3841 cggcatcata caacgacttg atgaaggaga cctcacaccg ctggtcggat atggtggcca
     3901 cgatttttgt ccttttaagc atactcgcta aatttttcgg tgcaaaacta ctcaatattt
     3961 agagaaacat aaatttttta aatagcgccc agaaccatat acgagggcga taacgtataa
     4021 atttaaaaga aattcagaaa actttgtaaa attgcctttt cgttatgttt atttgcagca
     4081 aactctgagt atggcgattt gtattttcgt tatacttata aatctggttg tattgtggtg
     4141 aaagaattgt aaaagaatgg ttttatggga tggagcgccg ctattgagga ttatattaat
     4201 tttttaaagc tggaaaaatc tctctctaaa aattcggtgg cagcttacca tacagacatg
     4261 atgaagttgt atggttatgc agagattata ggggtagagc ccgaatcggt taccggagag
     4321 caaattcagg actttattgc acaccttcac gatagcgggt tgagtaaacg ctctcaggcc
     4381 aggatagttt caagtgtaag gggcttctac aaatacttgc ttattgagga ggtagttgac
     4441 agcgatccaa ctgagcttat agagctgcct aaaaccgggc gaaagctgcc tgaagtttta
     4501 agcgttgagg agattgatgc catggaggca gccgttgatt tgagtatgcc cgatgggcat
     4561 cgcaacaagg caatcatcga aaccctttac agctgtgggt tacgtgtttc agaactggta
     4621 aacctaaggc ttaccgacct tttttttgat gatggttacg tgcgggtatt gggaaaggga
     4681 aacaaggaga ggctggtacc cattggccgt aaggctatag cggacattaa catgtacttg
     4741 tcgcagcgca taatggtaag cattactgtt gatgccaagt ctgggaacat cgtttttcta
     4801 aatcgtaggg gaaaacagct aacccgcgaa atggtttttc tcatcattaa aaaatatgcc
     4861 aaggcagcag ggataaccag gaatattagc ccgcatactt taagacactc ctttgccacc
     4921 cacttggtgg agggaggtgc cgatttgcgt gcagttcagg aaatgctggg gcatgaatcc
     4981 attctgacta ccgaaatcta tacccaccta gacaaggaat ttctccgtga aaccattttg
     5041 aaatttcacc caagaggtta actttgtgca aacatcctta aatcatttag ctatggatac
     5101 tggagaaaaa attgttaaaa ccctatcatc tgccggaaaa ccgatgaagg ccggagaaat
     5161 tgccgaggct gccggaattg ataagaagga ggttgacaag gtgattaaga agctggttgc
     5221 cgatggcaag ctccattcgc cccaacggtg cttttatgat attaaaaagt agctttaaac
     5281 cgggttagcc gctaacccgg tttttttaat ctttcgggtg atttttgaca cccttaacat
     5341 acaagggttt acatgaattt cggggaagaa aagttaagcg aaaactttac aataaatgtc
     5401 ttgcaggcat ggttttctcc tttaaagaat ttcaaacctg tcggcatcaa gatgagccgg
     5461 aaaacttgca cgaatttcat ggagttgctc caatgagatt gaagcttcaa tagttgattc
     5521 gctgttgggg tttgccttag ctacaatttg tcccattggg tcaactacct gagaatcacc
     5581 agaataattc gccccgctgc catcaatgcc cactcgattg caggcggcta caaagcactg
     5641 attttcgata gcccgtgcaa ccagtaggga attccacgca tagcttcgtt catccggaaa
     5701 gttagctacg ttgagcatca ggtcgtagtc attcctgttt cgacaccaaa cagggaaacg
     5761 caaatcgtag caaatggtaa gaagtatccg ccagcccatg tagcttacta ctaccctttg
     5821 gttaccggcg gagtattggt tgtgctcacc ggccatcctg aagaggtggc gtttatcata
     5881 gaaaacaacc tgtccgttgg gcataacaaa agccattctg ttcacgagtt tgttttcttt
     5941 ccgggcaata atgcttccaa caatagaggc tttccttacc atggaggttt gcaacatcca
     6001 atcaagagtt cgctcggctt cacttgcatc gcattccctg ggcttcatgg tgaaaccggt
     6061 ggtaaacatt tcgggaagca gaatcaagtt ggtatctgtt gaagtgcttt caattttggc
     6121 ggtaaaaagc ttaaggtttt cctctatgct ctcccaaaac ggcgatgctt gaatgatgga
     6181 tattttcatc gtgcagctta ggtgttggct attcggtata atgctgcgaa tatacctaaa
     6241 tttttttctg ggttaagttt taggcttggt ataggcactt ggctgttgtg aaaaagcgct
     6301 tggtcaactg ctcaatgctt aattgaacac tccccggaga tttctacctt tctttgcagg
     6361 gcttatttgc tgcttcctgt gaaactatgc cattcggctt ttgatttctc aaccagcctt
     6421 tcatactcca ttgatatagc gttaattaca ggtatggagg cgttcatgct cacgttacca
     6481 acctgtctaa cgggtagaac tcctggtgaa gttcctgtaa gaaagcaacc atccataaac
     6541 gccagctcct taatgcctat acatctttct actagttcaa cgttcaagct atggcatatt
     6601 tgcacaactt tttgtctggt aattccactt agcactttag tttctggcgc agtgaataat
     6661 ctatcacggc tgataaagaa aacattggaa cggcttcctt cggtaatttc cccctgggcg
     6721 tttaccaaaa gaacctcgta gcatccatga ctttctatga tctcgttggt aagctcgcgc
     6781 agcgatttgt tctcaacttt aaccgtcggg ttaggccttt cggcatcaat acattccaga
     6841 acaacgcctt tctgtttttc aattctggtg ggataccttg aaggaatgta gtaaaccgtt
     6901 aaattccgga ccggatgatt ggtggatacg ctgtaggtta tacggacatt cttttcagct
     6961 acagggtttt tctcagtgag cattctaagg gaactggcag ctgaattatt tgccaatgca
     7021 ggcaggttgg aatttttgat tgatgcttgc aggcgcaata aatgttcaat tgcaaaaatg
     7081 ggtgttgagc cagcaaccct tactacttcg taaaccaccg tttcgccctc aaccatgcta
     7141 tccgtaaaat tggtagttgg gaggagttgg ccgttatgaa caaaaaatcg ctcggttaaa
     7201 tcagctggaa aattcaaggc tttaatttgc tagtagtact ataactatca accatttagc
     7261 tcaggatgat ctcggatttc tgggaatgtc caggtaagtt gattcttgct ttatactggt
     7321 aaccctgcaa aaatagcagg aaaaactaat attcagcatg gatatttcta gtttgtttca
     7381 gcggagttgg gtaaaatccg aaggggtggg taaattaatt cctcatagtc tcgaaagttg
     7441 ggaaaagtgt taatttccct gttttctcag cctataaatt tggtaacggg gaattgatag
     7501 cagaatctat gtgcattggt ttgttcctca tattcgaatt aaaagttaac ctgtgctgag
     7561 gtatggacta gctactgcgt cttatttata tagctgttca gaatgttttc cattagcggg
     7621 gagtaaaact tgtagaagaa ttgagaattt tccgatttcc cttcaagcag ctggttcagg
     7681 aatttgcccc ccatcatttt agaggtgaac atggtaactt tgttttcggt aaagtctccg
     7741 gcaaagtaga aggtgaactc atttgattta gcttgtatta ctgcggggaa ttcattagtc
     7801 aatccaattg ccctgagttt ttgtaagccc tgttgggtag tgttaatctt aaaagttgaa
     7861 ggggtaacag cgttactacc gggaacaaca aattcaaacc aacccgcgaa tggaatttcc
     7921 gaaatcaaac caaagcgaga tattcctgta ttgtctgaaa taattaccgg gaaggcagaa
     7981 tttaaatctt caccatcaac tagcacatca actagcccat cattgctgag taagataatg
     8041 cccttacctt ctgatggcca tggtttaagg tgttgtgatt catacaactt gggcatccat
     8101 ggtgcagggc caccagcctc gttaggggtt aagttgcggc agaatactcc cgaccaaccc
     8161 gtccagctga cattgaatag ctcttcggct ttacttctca ttagtgcgtt ggttggagtg
     8221 ctgaacatat tatattctcc tatgatgagt tttcccatat ccttcatgct ctttagaaga
     8281 aggtagtcgt tctggttaag gccaccataa actttagagg aacgtatagg ttgggcatcg
     8341 tttttatacc attcaaaaga gtaaacacca taacaatcgg cgtagtatgc tgcatcgtat
     8401 gcggcagcat aggcatcaac ctcatttagc cgtatagcct taaagtcgaa tagctgttgg
     8461 ttcaaatcaa tggggaaaaa tccccagtaa tcctcattaa cccgataggt cttgccatcg
     8521 ggaccaacaa tcctgtcctg gttaagcacc caaaccaagg ccctatgctc tggccgattg
     8581 ctccgggtaa ctgttttgtc caggatatag atatttagtt tttttgaggg ttgaagctgc
     8641 cagaatatga acagcaagat tgccactagc ccaataacgc taatagcaac cattactcct
     8701 ttataaatct ttttcatcta tctaattatc ttatggttag ctggttgagg gtgaaaatgt
     8761 ttgtcaattc tgatgtgcta gagcggccta cttagcgctg ttcgttctga ctgtcggtta
     8821 tttgtcttac atccaaacgc cataaatggt taactttagg tagcttatgc ctgtatattg
     8881 attattttta aaagttaaac tccaaaacgt ttaaatccgc atggtttaag tacgatacgg
     8941 ccaatttacg aaaaattaat tattcggtgg ccaaattgtt gatggctaag acgatttttt
     9001 acttatactt ttaattttac agcccgaaga gccaatacgc ctgcatgtga ttagccttta
     9061 tacaggtttt gccacagcta accctcgggt aagtgcaaaa agttggatga gtggttggtg
     9121 cggaaatatt atttcttaga agttcgatga gttattatgc aagccaatgc cattaacagc
     9181 ttgagccaca tcagcggccg agtccatagg gagcctgcag gagctaccta cacatagatg
     9241 aaatttatgg tcaacctttt tagcgatcaa ctcaaaataa atgttagggt atattaccct
     9301 gcctaaaatg tcgttaacat cgtttgatgt gccctcgaat atggttatgc taacttcagg
     9361 aagtgcaagc gaaagcataa ggtttcccca gccatacaag aatactccgt tgtttttagt
     9421 attttcagcc aaattggcca gcacctgcat ggctattttg ctgtaaaggg ggttttcaat
     9481 atgcttagcc agaaaaagca aatttccggt cattatggag gatgaagatg gaataacccc
     9541 gtcaatcagc tccatttttc gaaccatcag atcactgctt cccggggtgt aatagaacat
     9601 acctttaccc tcatcccaaa aatcccgtat ggcctcctgc gctaatgaat ttgctttaaa
     9661 caaccatttt tcttccagcg ttgcctggta aagcataatc agggcgcgta tgtagttggc
     9721 ataatcgtcg agcagggcgt taccataggt tttacccttg cagaaaaccc gtttcaggtt
     9781 gccttcaaca aaatgtttgg tttcaagata gctggcaatt tcgctggcaa gcccaatgaa
     9841 acgtggattt ttaaaggtga tgaaagcctg cgccagggct gaaactaata aaccattcca
     9901 tgatgctaga actttatcat ctattagcgg aggaatacgc ttgtttctta tgctggcaag
     9961 ggtttttagg ctgcgctgca gcctgaactg aatctcctgc ggagggatat cgaataagct
    10021 gcttagctga tcatccgaag cgcaacgttt aagcccattt ctgccattaa tatctcccga
    10081 aggcgttacc ccatatgccg agcaaaaaat ttcgctatca aattccagga cttcgtcaag
    10141 ttcctgtttg gtccatgtgt agaagcctcc ctccagtcca ttcgcatctg catcaagcga
    10201 gcagtaaaaa ccaccatcgg gtgaatgtag ctccctgtcg agaaaatcaa ccgtgttaaa
    10261 cactatgttc aggtatgatg ggtttgggtt tatccggtag gccagggagt agagctcaac
    10321 aagctgagcg ttgtcgtaga gcattttctc aaagtggggt gtgtgccacc tggagtcaac
    10381 ggcataccta aaaaaacctc ctccaacatg gtcgtaaact ccgccattgg caatattatc
    10441 tagcgtagtg gaaacgaagc ttttaagttt tagattaccg gtagcctgtg cgctctgcat
    10501 cagatagcct agcagccccg gcatcgggaa tttcggggca ccacgcgttc cacccaggcc
    10561 gtaatctaaa gcaggttcaa tggattgcag gttccccatt actattccgg taaaatccag
    10621 ctccgtcagt ttcgtcttga catggattat ctcggtgttg gcaattcctt ctgccaagtc
    10681 ttccgctacc tcgtaaaccc tttcgggttc gctcttccag gtggcatcca tgctttcaag
    10741 aatattcaac cattgatctt ttgggaagta ggttccgccg taaacaggcc gcccgtcggg
    10801 aagggctatg cagtttaatg gccatccacc ccggcgggtt attatctgca cggcgctcat
    10861 gtagatgtgg tcgatatcgg gacgctcctc ccggtcaacc ttaatggaaa catacttctc
    10921 gttcatcaac ctggctacct gctcgtcggt aaatgattct ttttccatga tatggcacca
    10981 gtggcagctg ctatacccta tgctgatcac caccagctta ttttcccttt ttgcaacctc
    11041 aaaagcttcg tttccccatg gataccagct gactgggttt aacgagtgct gtaacaggta
    11101 ggggcttacc tcatttgcaa gtttattggg cttatcaaac attaactttt aggaatgtgg
    11161 gtttagtaac ttttggtatt gcatttgttc catttatttc aaaacaacta cccgtgcaca
    11221 atgttgatgt atgttccgag aattattcat tttgcaagtt tatttttctt ttttctattt
    11281 ttgaggtgaa aatattggtc gttaataaat tcatgtaacc atgaaaaatt tgctatttgc
    11341 cctatcgttt tctttccttt cgtttggtgt atttgcccaa aaccacaacg ggtatagaat
    11401 tgagattaaa attgatgggc tgaaggatac caccctgctt ttggggtacc actttggtga
    11461 taagaaattt gtgagcgata cagcacgtat agattctaac ggtgtagcca tttttgaggg
    11521 tgatagcttg ctaaaaggtg gaatttacct ggtaatccta ccggacaaaa cctactttga
    11581 agttctttta agcaacaacc agcgttttgg agttcaaaca acggctaaaa acatggttga
    11641 aaatctgaag tttacaggct caaaggagaa tgaagaattt attcattttc aacaatttat
    11701 gatgggtgtt caggctcaaa gcaatgagct atcggccaaa atgaaggatg cccttaccga
    11761 ttcaaccagg tcaaaccagc ttcggaaaga aatgaatgag ctggacgaga aggtgaaggc
    11821 ctactggctg gaggtcgaga acaacaacag gggcacattt ctggcatcaa ttatcaaagc
    11881 catgagaagt gttgaggttc ctgaatttac gattcctgct aacacgcctg cttacgattc
    11941 cttaaagtgg gttcacagct ataattttaa ccgcgatcac tacttcgaca atattgattt
    12001 cagcgattca cggcttcttc gcaccccaat tctggagaat aagataaaca tttacttcga
    12061 tagggtgctt ataccccatc ccgatagcat tgcagcggca gcagttaacc tggttgaaaa
    12121 atcgaaggca aacagggaga tgctccagta cattctttca tacctaatta acaagtttca
    12181 aacctcagaa aggatgggca tggatggcgt gtttgttgta ttagccgaaa agtactatct
    12241 tggaggccaa gcttggtggg ccgaccagaa acttctcgat aaaatttccg aaagggtaaa
    12301 tgcactaaag cccaacctta ttggtaaagt tgcccccgac ttatggctac ccgatccgaa
    12361 tggcaagtac tttaggctta gcaatattaa ttctaaaatt actgtaattt tcttttggga
    12421 tcctgagtgc agccattgta aaaaggagat gccgaagctt aaaagcgttt acgacaacta
    12481 caaaaagtat ggccttgagg tgtatgccgt ttacacccag ggcgaccagc ccaaatggat
    12541 ggaaagcatt tctaagaacg acttaacctg gattaatgtt tgggacccta cattcagctc
    12601 taatttccga aacctctacg acatttactc cacgcctgtt atttacgtcc tcgattccgg
    12661 taaaaaaatt atagccaaac ggattagcgt tgaaaccctc gaatcgattc taaagcagga
    12721 actcaagctg tagtgatttt ttcctcagta aaatttcagc ttaagggttg aaaaatcctg
    12781 aacattcccg taaatgattt tgaccaagat tagcagaccc ttcactgttg tgtctggtta
    12841 ttggtcatgg ttgtcacggt atatttgttg ataaatccga acagaacgga ttgttttact
    12901 ttcatattgt tagccaatat ccgaaattta taaaaattat aagctgtttt aggctagagg
    12961 atggaatttt tactatcttg gccgcaaaac tctaatcaac ttaacattta agatttatgg
    13021 agagcttact gaccaatacc tacttttggg tagtgttagc ctattttgcc gtggttttgt
    13081 ttatctcgtt ttttacaaaa aagcttgcta gccgatcgtc agccgacttc ttagttgccg
    13141 gtcgaaacct ggggcttctg gtgtgttcaa tggtggtagc cgccgaatgg ctgggagggt
    13201 tgagcacagt tggcgttagc gaaaaggcat tcgacaccct taccctgcaa cctattctct
    13261 ataatttttc cacagctatt ggaatggtga tactcggctt tactttagcc aaagcttacc
    13321 gcgaacggaa ggtgaatacg gtgagcgaaa tggtagagag catttttgga aaacgtgccc
    13381 gaagcatatc ggctattgcc tttctaattg cttttataac gcttgcctac gtggagctaa
    13441 ccacttttgc aactgttctt tcgccaattt ttaacataaa ttgggtttgg gccgtcataa
    13501 ttgcagctac tataaccaca atttacactt acatgggcgg aatgcacgcc ctcactattg
    13561 ttgctgtgtt caaccttgtt gttcggtatg ttgggttggg aattgcccta attattgggc
    13621 ttgttgcaat tggcgatttt tcaaatttaa ccaacaggct tgttgagatt ggcgctcctc
    13681 aaaatttgta caatcctttt agcataggat ggaatgctgc gttgagcctt atactgggtg
    13741 gaatttttgg tggaatggct tcccaagcca gcattcaacc tatttttgca gctaaaaatc
    13801 ctattaccgc aaaaagggct gccattttat cggcagtttt tatagctcct tttggaatta
    13861 tcacaggaat tcttggtctt attgcccgaa gtggtttgtt ttttgaagtt acccccgggt
    13921 ttaaccccaa aatggccctt ccacgcttgc taatgacccc cgaatttgtt catcccgtga
    13981 ttgggggaat tgcaattgcg gcaattcttg cggcaatttt atctaccatc gggccggtga
    14041 gcttttcaat tgttaccatt gctactaagg atatatacca tgggttgata aacaaaaagg
    14101 catccgatga gaaaatcctc cagtttgcta aaaggctcgt aatactggta aatctggtaa
    14161 ccgttccttt agccctgttt atgaagggcg caattcttga tgcagggtat ttttcctatg
    14221 ccattcgaac aattggtgca atagtgatac ttttgggtat ttacaaattg aagtggattt
    14281 ctaccctgtc tgttcagctt tcctttattg gcggcacgtt ggccatcatt attagcattg
    14341 tagccaaaaa gtttggttgg tttgacattg ataaaaccta tgccagcatc attgcaacag
    14401 tatttttcat tattataggc aacatctaca cgaagtactt tacaggtaaa caaaaatcat
    14461 cggagttaaa tatcaatgaa tcagctacta aatgacataa gggttcttga gctggccaac
    14521 gtgctggccg ggccaagtgt tggaatggcg ttggctgagc tgggcgccag agtggtaaag
    14581 gttgagaact tacttacctt gggtgatgtt acccgaacct ggaaattacc atccgaagat
    14641 cccgatacaa atatttcggg atacttcagc tgtgttaact gggggaaaaa gtcgttggcc
    14701 atcgatctca acaaacccga ggggcttgac attgtttaca ggctaaccga ggtttcggac
    14761 atcgtgctgg ttagctataa acccggcgat gccgaaaaac tgaaggtgga ctacaatacc
    14821 cttaaacaat acaacaaatc gctgatatac gcccatctaa ccgggtatgg attaaagaat
    14881 gaacgcgctg gatacgacgc aatcattcag gccgaaagtg gctttaccta catgaatggt
    14941 gaacaaggag ggaaacctgt gaaaatgccc gttgcactga tggatatact tgccggacat
    15001 cagctaaaag aggcaatttt gctggctctg ctgaatcgct ttagaaaagg tgaagggact
    15061 tatattgagg cgagccttat taaatcagga gttgccgctt tggcaaacca agccaccaac
    15121 tggttggtgg gaaattctat cccccagcgc atgggttccg atcatcccaa cattgtgccc
    15181 tatggaagaa ttttttatac ccgcgacgag aaacctgtag tgctggctgt tggcagcgat
    15241 aaacagtttg ccgagctgtg ctcaatcttg gggaagcccg aattggctca ggatcctaag
    15301 tactctacca acttcaaccg cgtcaagaat cgcaacgaga tttacccaat tttacaggat
    15361 ttagttgccc aaatagaccg tgatcctttc ttaaatgaac tcgccagaag gcggatacct
    15421 gcaggtggag tttacaacat gcaggaggtg ttcgaccaac ccgaagcgca ggagctggtt
    15481 atgagcggaa caacttcagc cggaaccccg ataaagggac taagaacggt ggctttttct
    15541 tacaatgaga attccaagcc cctagagttg gctgctcctc catattacgg tgagcataca
    15601 attcaaattc taaaggaaac gcttggtttc tcaaatgatt gtattgaagg tttaatcaat
    15661 aaaaatgttg tgtatgcacc caggtaaaac cagcttggct tttatagatg gatcgaggct
    15721 tattaccgaa tcgcttgttc gtgccggcgc cgataccttc ataggttacc caattactcc
    15781 tgcaaacttg ctatacctct acgccacaaa gagataccca acaatgcttg ctgctcccga
    15841 tgaaattacg acacttcaat ggatgagcgg ctttgccatt gctggacatg taccagttac
    15901 agccacttca ttcccaggtt atgccctgat gctggaatct ataaacatgg cggccatgtt
    15961 tgagctgccc atggtaattg ttcttgttca acgcttagga ccggcaacag gtacggctac
    16021 ttgtggggcg cagggcgatc tttcactggt tcacggtacc ctttccggcg gttacaccct
    16081 gccaacattc tcgccaagca acttaaacga ttgctgggat atttcagcta aagcccttca
    16141 tactgccatc aggctgagaa ctcctgttgt gctactcaca tcaaaggaaa tggtgatgac
    16201 ccttcaggct ttcgactggt cacatttggc ggagattgaa acacttaagg ttaaaacgtt
    16261 tagtggcagc gatacctaca ttccctacaa ggttgatgaa tcgcttgttc ccgattttat
    16321 gccagttagc caaaacaacc atgcagtgcg gttaaccgct tcaacacacg acacaaaggg
    16381 attccttcaa aattcaactc ccgaggctat agaaaacacg aagcggttgg aggctaaggt
    16441 taccaagcac cttcaggaat accttttatt tgaccttgat gagcaagcag gagctcaaca
    16501 tctggttgtc tcgtatggaa tttctgcaca agcttcacgc gaagcagtta ggcagctaag
    16561 agcaaggggc gaaaaggtgt cgctgctggt tgccaagacc cttctgcccg tttcacgtga
    16621 ttatgttgac attgccaacc gttacgaacg cgtagtggtg gttgaggaaa accttaccgg
    16681 acagctgcgc cagctgcttt ttggcgctgc tggtcgtaat gggttaagcg gtgttaatgc
    16741 tattgcccgt atgataagcc cttccgaaat tgttgacgag gtaatgagaa agttttagaa
    16801 attatttact tatgcaaacc ctaatatttg aatttcggaa atactagaat gaatcggtta
    16861 tatttaaatc ataacgattt ttagaatgaa caacgtaaaa tttttaaccg gaggacaact
    16921 acccttttgt aagggctgcg gacatgttgc tgttgcccag aacaccgaaa aggccttaca
    16981 aaagctagat tttaaccccc ttgacgtagt tctggttacc gatattggtt gccatggcat
    17041 tgttgataag aattttctca cccacaacgt gcacggcctt catggacgct cttcggcctt
    17101 agcagcagga atatcggcag gattaagtaa cccgaataag aaggtcatag tttttacggg
    17161 cgatggaggt gcaactatcg ggatgcagca tttagtcggc ggagctcatc ttggattcga
    17221 catgacggtg gttgtgcata acaacatgct ttacggcatg accggtggac aaccctctga
    17281 gtttacaccc tgtggcttca aaactccaac ccttcctgaa ggcagcacaa agtccggcta
    17341 cgatatttgc gaattaatgc ttgctgctgg cgcagcttat gttgagaggg tggttggaat
    17401 aggcgatttt tctgattctc ttgcacgcgc tttctctacc aaaggcttct cgctggttga
    17461 ggtgatggaa atttgcacca gctacggggt aaaatcaaac cccggcatga agcttcccaa
    17521 gctggttgag gaagccggat ggaaggtgaa agtttttacg gaggctaaac agcgcctctt
    17581 tcaaacccca caaaatagta atcctaccag cctgctttcc gaaaaacttg aggtagagcc
    17641 aaaatactca ggggcaataa gtaagcctgt aagcattatg ctgtcaggat cagcaggcga
    17701 aggtgttcaa ttagcagctg aatttctggc cagagcagcc atgctgtcgg ggttgtatgc
    17761 tactaaaaaa ggaagttacc cggttactgt cggagttggg tattcagctg cagaggtgat
    17821 aatttcacac gaacccatac tttatactgg ctctcctgtt cccgatatcc tggctattac
    17881 ttctgccgat ggacttgggt atgccagaac tgcagcagga agaatgaaag gggggacaat
    17941 ttacatagac caaagtcttg atatacccca aacaggtgct gaaaccgtcg tgattccctt
    18001 tagagaaaag gtgggcgctc aaaatagttc gctgtacagc gcattttaca tggttggctc
    18061 ccagcatttt ttccccatgg aggctttaag ggatattttt atggctaata aaatttcgca
    18121 acgggttagc gtggatatct ttatgcagct ataattcaga atgatgcaaa gcttgttttg
    18181 gtggaaatac ctttaagttg tttccagtgg tatggtatgt cgggcatata cacactactg
    18241 gcaagatgca agttcttgaa cacgccggga atggatgaag tgctagcgaa gggcaggggt
    18301 gttgctgatg gctgcaaatc tggaaaaagc cataagcaag ggcagcgatg cagcatttac
    18361 cctgcaaaaa gccagcagag ggcatagcag cctacaaggg ctgaatcttt tgatgtaggg
    18421 agcagccggt acaaaagtaa atccctatgg agcggaagca agctatagcg catccgcagg
    18481 agtttttttt caacagcagc gggaacgcta tactccagtg aagccgtaag cggggctggg
    18541 ggcagtaaag tttcagcagg agggtttagc aggagtacag cgacaagact taacccacgg
    18601 atttatgaga ggttacacgt taaaaggaat tgccgtgtcc acctcaggcg gatgccgggt
    18661 ggtgtaagag ggagggggag taccccccta cctaaccgat taataaggta tgcagatctt
    18721 gcttgcgctt ttagtcgtta ttgtttagaa acccaaggta gtagaaaagg gtggccaacg
    18781 agcctagggc tgcaataaca tacgtgaggg ctgcccattt tagagcatca cgtgcgtaag
    18841 ctacattttg accgtacaga attccggttg agcctagcca ctggagcgcc ctacgcgatg
    18901 catcgaactc aacaggcaaa gtaatcaggc taaacagtgt tgtaagggcc agcaatgcca
    18961 cgcctacaac cagtatggcc ggcatagatt gaagcagaat gactccggca agcaaaatcc
    19021 attgaaccca tcgtgaagct acacttacaa caggtacaag ggacgaacgc agctgaagaa
    19081 aggcgtagct ttgtgcatgt tgaaccgcat ggccgcactc atgcgcggca accgcagcag
    19141 cggcaatgtt gtgtccgttg tacaccgcgg ggcttaaatt tatagtcctg tcagttgggt
    19201 tgtaatggtc ggttagctga cccgtggttg atattacgcg cacatcaaaa ataccatgct
    19261 gctttagcat catctcggcc acctccttgc cggtcatacc ttgggggagg tcaatcttcg
    19321 agtaccgtgc aaaccttacc tttagctggt tggatatcag gaagctaagc acgaaaaaaa
    19381 taatcattat caggtatatg cccatggtgt ttaattttta ttttggttag aatttcatcc
    19441 tttacctacg tttatttact actaaaaaca gaaagtggct caacggcaag tttctcggcc
    19501 acttcgttaa gatcgttgag aacttgaggc aatattgtaa taccttcctt tgattttatc
    19561 ctctcgtttt ccctttctgg atcaccgggg atgagtactg gcggctggtt taccccgctt
    19621 tttgctgcac ggaatgtttt aatccattcg tccatcatta gcttaaactc gttggctggg
    19681 cgaaaggcat caatacgaat agcaccgaaa aaatgcccaa gtcctttgcc aacggaatgt
    19741 tcaggaatgg ggaggtaggg aacctgtgga ggcacaaatg ggccaaagtt ggcacctgaa
    19801 agtacggccg aaaaaatatc aactattgag gtcaggcagt atcccttgtg gcttccatgc
    19861 tcatagtctc ctccaagcgg tagtattcca ccgccgtgaa gcaaggtgtc cggatcgtcg
    19921 gtggactttc caagtccatc ctgaacaagt ccgataggtg ctttttcgcc ctttttagcc
    19981 attagagcca gcttgccgcg ggctattggg gctgtggcaa aatcggccac aaagggtggc
    20041 tgttctaatg ctggaatggc cactgctatg ggatttgttc ctaaaagctt gctcgttgaa
    20101 aaggttggaa ctaccagggg attggcattg gtcatgctaa taccaatcat atcctgctca
    20161 agcgccatca tggcgtagta tccagcaatt ccatagtggt tgctgttttg ggttgcaacc
    20221 catcccgttc cggcaacttt tgccttttct attgccaggc gcatggagta aagtcctgct
    20281 acaggcccca gtgcattatc accatcaact gtagcagttg atggagtttc atgtatcgtt
    20341 tttacagatg gcctggtgtt tatcctcccg gcagccacca tttggtagta gtccttcacc
    20401 cgcattactc catgtgatgg aattcctcga agttctgctg ctaccagtag gtctgcaacg
    20461 gattttgcat ccatttccga acaacccatc cgctcaaaca gcaggctaat gtagctctta
    20521 agtttaagat aatcgatcat aaatggcaat ttatctttta caaagataat gaatgtaagt
    20581 atttttagag gtctagctat tggttccgta tagccaactg tgtaacaaaa caaaccgtaa
    20641 taagcttact gtcaaggtat aatattttgt taagtgtttg ccggtttttt aatttaacaa
    20701 aatttaaatc gctttgaaag gttaaacgtg caatacactc aaaaaggcat aatggaaagc
    20761 aaatcacaag cactatagaa gttttcgata ttacaacctg tagctgtttg cgggcgtttt
    20821 ttaatatgat tgatggtgag tggatttgat gcaaaggata ttaggttcaa tacatctttg
    20881 agcaacactt gaagaatgtt tataccctgg ctaaccaaga gaacatagct ataatttttt
    20941 gattaaaatt gtgcgaaatt ttttgttatg acctataatg aagcgttaga ctacattgtg
    21001 tcgcagctac ccatgtttca tcgcgttggc aaagctgcct acagggcaga cctgggtaat
    21061 gcgttggtgc tggatgaatt ttttggtcat cctcatcaaa agtttgcatc cattcatatt
    21121 gcgggaacca atggaaaggg cagcacatcc cacatgctcg catcggttct tcagcaagct
    21181 ggatacaagg ttgggcttta cacttctcct caccttaagg atttccgtga gcgaataaga
    21241 atcaatggtg aaatgatttc acaggttgag gttactcgat tcgtggagga gtatcgggcg
    21301 cttttcgaca gggtaaaacc ttcgtttttt gagatgactg ttgccatggc attcgactac
    21361 ttcgcacgtc gaaacgttga ggttgctgtg gtggaggtgg gacttggtgg aaggcttgat
    21421 tcaaccaata tcattacccc aatcctttcg gttatcacga gcattggtct cgaccacacc
    21481 gatttactgg gaagtacact tcctcaaatt gccggggaaa aggcaggtat aataaaaagg
    21541 ggtattccgg ttgtcgtaag ccaacttcag cccgaaattg ctgatgtttt tactagtaag
    21601 gctggtgaaa tgcaatcacc tcttgttttt gccgactcag aatataaagt tcgagaaatg
    21661 agctattccg cttctggcac gcaaaccttt ctggtggacc atgggggaaa tcctgagcag
    21721 tgggatacag atctcatggg gagctaccag cgattaaatc taccaggggt tcttgccgcg
    21781 cttgatgttt taaggaatga aggatacagg attacatccg atgcccagaa gcagggtctc
    21841 aggatggtgc aacagctcac cggcctgatg ggtcgatggc aaaaacttca ggataggcca
    21901 ttaatatact gtgatacagg acataacgta gatggtataa ccatggtgat ggagcaaatc
    21961 caccggatga gctacgctaa gcttcatgtt gttttgggta tggttgccga taaggacatt
    22021 gatggcatgc ttagcatact gcctgccgat gccatttact actttacccg tgcagccctt
    22081 ccccgtgctc tggaccagaa tatattgaag actaaagcca agggttttgg attgaaaggt
    22141 gaagcatatc ctaccgttgc ccaggctctt gaggcagcca agggaagtgc taatgaggac
    22201 gatcttatct atgttggcgg aagcactttt gtagtggccg aagtggtgta gtgatatttt
    22261 ttcaaaaaaa aatttgctga tttaaaattt taggctacct ttgctccgca tttcaaacga
    22321 gagtgcagtc aaaataaagt attgggagct tagctcagct ggtttagagc acctgcctta
    22381 caagcagggg gtcataggtt cgaaccctat agctcccaca aaaaaaattt aaaccgcata
    22441 cattctttca atgtaggcgg tttttttatt ggatgatttt tttgcttacg gaagagtatt
    22501 ctacaaaacg tccagtacat gttcaagtcc tacaccccgc gaacccttta aaagaatggt
    22561 tgcatttgcc acttcgtggc ttaggaagta ctcagcacat gctttggcgt ctgcgaacca
    22621 gccgtagctg ctatttattc ccgaaaagtt ttgtccaatg agaaaaacct gtgttgcccc
    22681 aagcgattca agcagtttaa ccactttctt atgttcactt tcggcgtagc tgcctagctc
    22741 cagcatttcg ccaaggataa ttatcttatt gggactctca agagaggtga agttgcgtac
    22801 ggccgcctcc atgctggttg ggttggcatt ataggcatcc attatcaccg tattgcgctt
    22861 tgtttgtata agctgtgaac ggctgttaga aggattatac tcctcaatgg catttttaat
    22921 aagttccggc tttactccaa agtgaaggcc aacagcaata gctgcttcaa tattttcaat
    22981 gttgtagctt cccaccagct ttgttttaat gtccatgggc ttatctgtac ctgccatttc
    23041 aacattaaca gaaatgaatg gtgtatcatg ctggtttgtt acctttatgc ttttacaata
    23101 gttggaatac tccagcttgt tggctatgcc gattcccatg acaagctgtt taagcacttc
    23161 attgtgctgg ttgtagataa tcaaaccctt gtgccgggcg atgtagtcat acaactcgcc
    23221 ctttgtcctt accactcctt cgtacgaccc gaaaccttcg aggtgtgcct ttccaatgtt
    23281 ggtgattatc ccgatatcgg gtgctgctat tgaggctaac tctgcaatat ccccgggatg
    23341 gctagcaccc atttccacaa tggcaacttc tacatcagcc gtaagggaaa gaagcgttag
    23401 cggaacccca atttggttat tcaaatttcc ttttgtagca accgcatggt atttttttga
    23461 gagaacgcat tgaatcagct ccttggtggt ggttttaccg ttggtcccgg ttataccaat
    23521 tattttggct tttagttgtt tccggtgatg gttagcgagc tcctgtaggg tttttagggt
    23581 gttttcaacc aaaatgcaat catgcgtcat aaaggttgga ttgtcgatta cagccaagga
    23641 tgctccatta tttaatgctt gttctgcaaa cttattccca tcaaatttgt ctcctttcaa
    23701 gccaaaaaat attgcacctt taggaatgct tcggctgtcg gtggcaactg agccatgatt
    23761 taggtagtgg tggtagagtt cttcaagcat gggttgggta tttatatcct gttttttatt
    23821 attaagttga ttaacttagg ttctttactg ggtgtgtatg tggttgatgg tgatggtaaa
    23881 cttttatagc ttggttgtag ggtaggggta atatacaaat gtaaggcttg tcaatggcat
    23941 cggaaattct tttaaaggtt ataccttcaa aaagcagaaa acctcatgtt tatgaggttt
    24001 tctcgatatt aattgaagta tttccaaaaa aaacgttact cgcctctttg acgggcagac
    24061 tgggggccaa cagcctccat tgcgcaacga aatccgatat cgttggttga agaggcttcg
    24121 tccagatacc ttctggtggc gggactcaac cagtaggctc tatccttcca ggaaccaccc
    24181 ttgtaaactc taacatggtc gttaatgagg ctggtcatgc cggcaccttt tgcatcaggt
    24241 ttaccctgat cgtacatggc gttagaatct ttggttttaa gggtttcccc ttcgacgtag
    24301 tttagagtag aagcaacgtc gccatcttta tagttaatat agtaggcctt gttatagttt
    24361 cgcctgttgg cagcttcttc ctcggtaacc tccctgtatg gaattctacc caggctatcc
    24421 ttttttgcaa agttgccttc tttatcatag ggggttttga atacatttcc acgaaatggt
    24481 ctaatctcgt caaagtcttc gtagcttaaa ggacggtaaa cgtcgagcac ccactcgttg
    24541 acattaccgg ccatgttgta aagcccgtaa tcgttaggcc agtaggactt aacaggacca
    24601 gggaaggtat tctggtcgtt taggtttcca gcaagtccca tgtagtcgcc gcgtcccctg
    24661 acaaagtttg ccatcatttg tcccctgttc ttagattcag agtttctaac gttgtggcca
    24721 ttccatgggt agatgcggcg ctcaaccatt cgttcttcgt aggtattccc aactaatcct
    24781 gaagctgcaa attcccactc ggcttcggtt gggaggcggt attttggcag caggatgcca
    24841 tcttcaaagc gaaccggacg gccttcgggc tgctcggggt taaggcttgg caaattttcc
    24901 ttaacttttc ccacatattg accggcaagg taggcttcgg tgttgaaaac atcatcaccc
    24961 ttttgcccga gttctatttc aagaattccc ttttgaacaa gcagcatttc atttacccga
    25021 tcggtacgcc actggcagta gtcatttgcc tgaagccaat ttacgccaac aacgggatag
    25081 tcgttatatg cagggtgacg gaagtaatcg gtaacgaatg gttcgttata gcccaatggg
    25141 gatcgccaaa ccagcgtatc tggaagagcc tgctttatta cctgcgggta gtccgaataa
    25201 acgcgtttaa gccagtatat gtattcccgc cagtcaacgt tactaacctc tgtttcatcc
    25261 atgtagaaag aggttacggt tacccttctg ggcttggcat tccaatcgta cattaagtcc
    25321 tgttcaaccc gacccataat gtaggttcca ccctcaatga acaccaatcc tgggccggct
    25381 tccggtttcc agtcggtaac aacttcaaaa ccaccttcgt cgggcgaatt gtatgcccat
    25441 ccggtttttg atgatacgtt tgaccccccc ttaccagaaa ttccacatga agtaagcccc
    25501 aacatggcta ccaaaataag gcttaatggt ttattcttta agttcataac tatttatttt
    25561 tacccttaaa ataagttcaa atatagtgat aaaaaattgt ttaccacaat gtagtaacgt
    25621 aaaaaattaa aatttcggac atttaatgta cttatattta ctttttccct ttttatactc
    25681 aaaattccat cccagggtta cctcatgcga tgaggttggt aatcctctaa ggccatactt
    25741 cattatgctg aagtcgtagc tatagcccat tctaaacaaa tcgtcggtgt acccgatcgt
    25801 gaaaataaat gaatggctgt tcaggcttag gttttccctt agccaaaatc ctcccaccaa
    25861 ccagtgccgg gaaagataga acccaatgtt tagctggtta aacttggctt gttgaatgta
    25921 tattatgttc ggtgacagca ggagaacctc tttaaatttg taccatttat acaggttgaa
    25981 gtcagcgcca atatgaacgg tgtacttccg tggtatgtaa gcttttgtgt cctggtaggt
    26041 tgcctccact ggctccagaa ggtggtgaac agctattcct ccataaaaat tttcaaaatc
    26101 ccccgctact ccaaggctga aatcatagtt ccatgttgtt attcccgaag tattataagt
    26161 accggttaca tctccattta tatcgatcat atccggaaat acaagccccg agtagttacg
    26221 ggtacgcaca atgccacttg tttgaatacc aaaacgcaaa tgtgagttat aggaaggctg
    26281 aatcccgtaa gcatagacca aatctaccgt ggtgcggcta aacactccct tgccctccac
    26341 atcggctaca atattgagtc ccacaccgct ttttatagat tgaattgtcc tgtcaaagct
    26401 aaccccatag gtagagtagg gcatatcaat catggtccat tgatttctgt aaacaactcc
    26461 tagtcgcatg tatttgctag cgccggcgta agcggggttt agataaagag ggttgaaaaa
    26521 tagctgggaa aatattggat cttgcccaaa tacccttatt gatgtaacca ttgttatacc
    26581 tatagcaaca aaaaattttc taaacatttt attatcagta gctaaatatt ttgttgataa
    26641 taattatgaa tcctttgccg tttttattgt tcttgtaact cgtaaaagta aaataaagtt
    26701 tttatttatt tgcaacagca aaatcaattt ggtgatgtgc atgtttagaa gaacgttttt
    26761 tctttcgctt ttgtgtattt caaatggcat ttttgcacag gatactctgg agcgtgcaat
    26821 ttgctggaat tacaggcaag tacccactgc tcaggctgag cagaccatac tgtggttcga
    26881 taacgccgtt tacacaaatc cacagacaat gattccttca tactttgagc taattccaat
    26941 cgagaataat ggtgcataca attcaacacc ggatgttttg gtggctgaca agcaatgggt
    27001 tttgttggct aatgacgaaa tgaacctgtt gcaaggcgtc aataacttta taaatgaaag
    27061 tgttgactgc agaacagcca ttcaaagggg aaggatgatg attcaactta ctgttccggc
    27121 ggtgaggctt aattctgaca acaagtttga gaagctggtt tcttttaaaa ttatactaaa
    27181 aagggcagaa aaaacaaatg atgaacgcag ctatgcccct gctcgtctcc aggtcaaatc
    27241 gtcggtgcta agttccggta gctgggtaag ggttagcgtt cgggagggtg gtgtatataa
    27301 aatttcatac aacgatcttc aatcgtatgg gctgtcaaac ctcgaaaatg ttagcttatg
    27361 gggaaatgga ggacactgtc ttccctatat gaatagcgag caggcacctg acgatttaaa
    27421 tccaattcct attctcatga gtaagggtga tgacggaatt tttaaccagg gcgattacat
    27481 ccttttttat gccgaaggcc ccaaaacttt ttcctacaat gctaccatgg acatgtggga
    27541 ggagaaaagc catccttatg cccaacaaat ttcatacttc atcacaactt ctttgcctca
    27601 atcaacaatt gaaaccatag aagagtcagc tgctccatta aaccgcacgg ctaattcata
    27661 tgatgcattc ttaaccattg aaagaaacga taccaacatg gttaaatcgg gtagggagtg
    27721 gtttggcgat gttttcgatt taactacatc ccgaacatat actaccgacc tttccctacc
    27781 tgttgtgggc ttacctttaa aagtttggct tagggcggca gcacgctctt ctgtagccag
    27841 caactttacg gtaaaggtca acacaaccac ccttggaact ttgcctttaa acagcgtagt
    27901 aattggtgat gagtacgccg atgtggtagc agtgaatgag caggtttaca cctctgcact
    27961 taacgaaggc aacctgacaa tcgaattaac ctatagtaaa cctaattcta ctgcaaccgg
    28021 cctgttggat tttatcacaa taaatacccg ccagcagctt aaatataatg gttcacagct
    28081 gattttcagg gataaacaat cggtcggaag cggtaatata acggaattca gcatacttaa
    28141 ttcaaaccaa aatgttcagg tttgggatat cactagtttc aaccatccct acaggctcaa
    28201 ctccaggtat tcgggaggag tagtagcatt taaacaggcc accgataccc tacgccattt
    28261 cattgctttt gaacctgaaa aggctaagac cgttactttt gttggagctg tggctaacca
    28321 gaacattcat ggagctggac agcccgattt cgtgatagta gcccatccgc tattcatgcc
    28381 acaggcacag gagctggctc aacttcaccg ctcacttgat gggctggatg ttgtggtttt
    28441 ttcaactgat gaggtctata acgaattttc ctcagggaat cccgatttgt cggctattcg
    28501 caacatgatg cgatattttt acaggtgtgc tgctacaacc gagcaacaac ccaagtacct
    28561 tcttttgttt ggcgatggat catacgataa cctatccgac aggaatggca acaccaacta
    28621 tattctaacc taccagtcgc cacgttcaat caacaaagtt ggttcgtttc agtccgacga
    28681 ctactttggg ctgctcgatg acgaagaggg tgaagctgtt ggcctgcttg atgttggagt
    28741 aggccggttg cctgttagca ctaccgagca ggctaatgct gttttagcaa aaattcagca
    28801 ctatgtaagc ggcagtaaca ctggtgactg gcaaagtaag ctttgtttta taggtgatga
    28861 tgaggatgga aatattcaca tgacggatgc caacaccctt gctgattacc tttcaaccag
    28921 taatccagaa tacaacattc aaaaaatatt ttttgacgcc tacaggcagg aatcttcatc
    28981 ggggggagct agctacccgg aggtaaccaa cgctataaac acaacggtga acaacggggt
    29041 attccttgta aactacacgg ggcatgggaa cgaaaggtgg cttgcccacg agaaagtgct
    29101 gatgctatct gatgtatcat catggaaaaa cacaaaacag ctgcctcttt ttgtaacggc
    29161 cacctgcgag tttagccggt tcgacgatta ccacctcacc tcaaccggag agcagattct
    29221 actttcgcct gacggtggtg gtattgccct ggtttcaact acacgtctgg tttactccag
    29281 ccctaacttt actttaaatt ataaatttat caacaatctt ttcaagttta atgaggactc
    29341 aacctcccat taccgcttag gcgacctcat caaaatatcc aaaaaccttt ccggaacggg
    29401 taacaacaag cgcaacttta ccctgctggg cgatccggct ttaatgcttc gataccccga
    29461 acttggagct gaaattacca gcattaatgg aaaacctgtt gatgaggagg ccgataccct
    29521 caaagcttta tcggttgcaa acctctcggg aaggatattt accccgattg gccaggttgc
    29581 atcagacttt agcggaaatg ccaccataac tgtttttgat aagaaaagcg aaattaaaac
    29641 gctggcaaat gatggcggga tgccaatgac cttttttagt tgggacaatg tgctctataa
    29701 aggcagagct attgtcaata acggattatt cagcattgat tttgttgtac caaaggatat
    29761 tcgcctatcg tttggcacgg gtaggataag tatttttgct gacgacttgg caagggtggc
    29821 aatgggctac aacgaatcgg taattgttgg cggggttttc aactcacctt ataccgataa
    29881 cgaaggaccg gagattgacg tttaccttaa cgatacactt ttcaacgggg gaggaatatc
    29941 cgatagcagc cctaagcttc ttatccggtt aagggacgag agtggtatta atacttccgg
    30001 aattgggatt ggacacgatt taacggcaac ccttacttcg cctggtggcg atgaggttaa
    30061 cgtaattcta aacatgtact accagtccga acctaacagc taccaggagg ggtatgttaa
    30121 ctaccagcta gctgaccttt ccgaaggaag atactggctt agggtcaagg catgggatac
    30181 ctataataat agctctgaaa aaattatttc gttcgttgtg gttggagcag gaaaccttca
    30241 gctggaaaaa ttctactctt accctaaccc attttctgag ggaacatctt tctactttga
    30301 acataacatg ccggaagatg agttcaacct tacaattcaa atttttgacg tggcaggacg
    30361 gctggttagc acaattacac agtcacaggc ttactctggg ggataccggg ttggacctat
    30421 ttactggaat ggaactgaca gcaggggaag tcggcttgga aggggcatat acatctgccg
    30481 cttatccgtt aaaacttcta gcggatatgc agccagctta cagcataaac ttgtcatttt
    30541 gaggtaaaat ataattccga gttttacgtt cgctagaata tatttcacaa tcagctaaat
    30601 ttgcacctaa aatttatatc agatgcgtag aatactgttt acacttcttt cttttgttgg
    30661 cttcattttt ttttcacaag tctcaagtgc acagttcgat agcactgttt cgcttaccgg
    30721 cggactgaac gctctcaaga ttgccgttcc ttttctaact attgcacccg attcaagagc
    30781 tggcggaatg ggcgatgctg gtgttgccac ttcacccgat atcaactccc agcactggaa
    30841 tccggctaag ttcccattta tcgaaagtaa aaaatggggt gttgcctttt cctatacccc
    30901 ttggcttaga aatttggtga atgatatcaa ccttacctac ctcaccggtt actaccaggt
    30961 ggacaagatg caggccatca gcagctcgct gctatacttc gccatgggcg aaatccagtt
    31021 tactaccgac aaccccaatg accccccggt taaatttttc aaccctaacg agtttgccat
    31081 tgacattgct tactcccgca aattttccga ttacatttcg ggtgctattg ccttcaggta
    31141 catccgttcc gacttgagcg gtggctactc gcaacccggg caagcctcgg ctaaagctgg
    31201 aacttccttt gcagccgata ttgctatgta ctatcaacat cctgtaaagc taagcgataa
    31261 atttgggcaa atggcttttg gtttcaacat ctccaacatc ggcacaaaac tttcatacag
    31321 cgatgacccc atgaaggatt ttataccgat taacttgcgg cttggaggaa ggttgaccat
    31381 ggatatcgac agctacaact cattctcctt tactgtagat gccaataaac tactggttcc
    31441 aactccaccc gtttacggag ttgtagatga gcaaacagta atagttaagg ggatgaaccc
    31501 caacgtgtcc gtcgtacagg gaatgctaca atcgttctac gatgcccctg gtggttttaa
    31561 agaggagctg aacgaaattt actatgctac aggcattgag tactggtatg cccgtcagtt
    31621 tgcccttaga gcagggtatt tttatgagaa tgaaaataag ggtaaccgta agtactttac
    31681 tgttggtgca gggctgaagt acaacatctt taatattgat ttttcctacc ttattccgac
    31741 cggtggattt aacagcccaa tggcaaacac ggttaggttt tcgctatcct ttgactttga
    31801 ggctacacgt atgcgtggcc aaaaagtcaa caacaactag cccatgaaag ttagagtggg
    31861 atttggctat gatgttcata agctggagag caacacccct ttccacctgg gtggaatatt
    31921 tatcgagcat tctaaaggag cagtaggcca ttctgatgct gacgtgctta tccatgctat
    31981 ttgtgatgcc atgctaggcg cagcaaacct cagggacatt ggctaccatt tttccgacac
    32041 taaccccaac ttaaagggta ttgatagcaa aattttgctt gctgaaaccc taaaactact
    32101 tgatagcaag ggtttcaggg tagttaacat tgattcggta gtgtgtctcc aacgccccaa
    32161 gcttatggac atcatcccca acatgcagct aaccctggca tccgtcctta aaatggacat
    32221 ggaggatatt tctattaagg ccacaaccac cgaaaagttg ggatttgttg gaaatgaaga
    32281 gggcgttact gcctatgccg ttgtcctgat tcaaaaggat tgacccacat ggtacctgaa
    32341 aatgcagaaa cgttttctac ccttgttctg ggggccagct gtaaagcagg gaggttttcc
    32401 tatctggcca ttaaaagcct tgtgaaacac ggggttaggg tctatgcaat tggctcgagg
    32461 ccatgctttg ttgacggagt tcaagtacat gcagaaaaaa ttaacctcac caacctccat
    32521 acggtgaccc tttacctgaa cagtcaaaat cagacaacct actacgacta tattttaagc
    32581 ctaaaaccca aacggattat ttttaatccc ggtgctgaaa accccgagct ggtgagatta
    32641 gcacatgaaa acagaataga ctatgttttt ggttgcacct tggttatgct taacgatggg
    32701 agttttttta aatcatagat tcccattaaa cattctgcag caaccctgct ggcaaattcc
    32761 atgctactaa ttattaaaat tgataaataa cttaatttca gctatttacg aatagcactt
    32821 gccaaggaga aaagccatgt ttttaaaatt ttgttaaaat atttatgttt ttttaataca
    32881 taagcttaaa acttatgtaa atttgcatcg gtaaataaca attaccaata gatatttaac
    32941 ttcattaacc caaacaattc gattatgaaa aagacagttt tactaggatt cgcagcctta
    33001 agcatggttg cagttctttc tggttgtggc aaggtgcccc aggagaaaat tgatgctgcc
    33061 aatgcagcta ttgactctgc caaaatggtt gaagctgatg tatatgttgc agccgatttt
    33121 gcagcagttc aagattcgat gagcgctgtt atggccgaca tcgaagttca aaaatccaaa
    33181 cttttcaaga aatttggacc tgctaacgaa aagcttgaca gaattctgag ccaggctcat
    33241 cagattcaag ctaacgccgt taccaagaag caggaagtta aggtagaagt tgaaggttta
    33301 atgaccgaag taaccacctt aatcgaccag gacaaaactt tgatgaaaaa agctcctcgt
    33361 ggaaaggaag gcgctgctgt tcttgaacaa atcaaaaccg aaattgccgc tatcgaagct
    33421 agcgttgctg aagccaaaac aatgtacgac aatggaaagt acatggatgc ttccaacaag
    33481 ctgaaagctg ctaaggatag cgctactaaa atcaacaccg aacttaccga tgctattgct
    33541 aaggtaaaaa agagatagta taattcgatt ttccgaaacc ccgaggttgt taaacttcgg
    33601 ggttttttat aggctattct tatttagtta ccatgtttta attttggacg aggattttca
    33661 cctcaacgaa tatgctagta atattgatag attagccaat gtatgctaat tttgcatact
    33721 gtaactgatg gtttttacat agtggcttgt tcatgttgct cggaaatagt tggtttactt
    33781 aggttttata aatcattttt ctataaccta atttattcgt tatcagccta ttaaatagga
    33841 gtttatgagt agaaaatcga aaagaatagc tgttattata ctggcgagtt tggtcgtggc
    33901 taccggcatt tctgtgctat tctacaagtt aatacctgaa cctccgattg gagatgttga
    33961 ctatgcccgt ttatgcctgc agcaagcctc caagagtaaa gcacagagct actctaaaaa
    34021 aagttttaaa caagccaagg ccgactacga ttctgccatg aagtgctggc aaaaggaaaa
    34081 tgaaaagttt atcctgttcc gtgactattc catggttgca aaatttgccc aaaaatcggc
    34141 gaaagaggca agaaatgcca tgaacaattc ccaaaactta aggaagaaca tggttgtagc
    34201 tgttaaggat aaaattgaac tcctgaatgg cctgatacta aatgtaaatg agcttttcaa
    34261 cagggtgcca cttccccagg aggtaaggga taagatttca cgggggaagc tgctgcttaa
    34321 ggaagcccag ctatattttg aaaggggtga gtacctgtca gcccaggaca aaattgttct
    34381 tgcagaagat atgctcatgt catcctacaa tagaacactt gacgacttgg aagagtactt
    34441 taactcccaa ccatactggc gtaagctggt aaatagcacc attgagaact ctgcaaaaac
    34501 caacggaaac gccattatta ttgataagtt tgcccgaaaa ctttatatct accagtcggg
    34561 tgtgaaaaaa cttgagtttg aagctgagtt tggtaaaaac tggatgggta gaaaaaggct
    34621 taaaggcgat aaggcaaccc ctgagggtat ttaccttgta aaaaccaaaa tcccccagaa
    34681 taaaacgaag tactacaggg ctcttctctt aaactacccg aacaatgacg atgttgaaat
    34741 gtttagaagg gaaaagcagc aaggtaaact tcctgcaaat gctcaaatcg gcggcttaat
    34801 tgaaattcat ggtaacggcg gtaagggaac agactggact gacggatgca ttgctcttag
    34861 tgataatgat atgcttaagg tttacaactt tattcaggtt ggaacaccgg ttgttattgt
    34921 aggctcttta gccgatttta aaagcattac cgaaaatttc aagttgagaa aacatacaga
    34981 aaggagctaa ttatatatgg atgccgtaaa tatagccctt gccactaccg ataaagacaa
    35041 atcgattgga aaagtatcaa aatttaacaa tatcctagcc ggactgttaa ttgtgattct
    35101 ttcaattatc cttttttttg ccatacttat ttacgctatt ccctcattaa ccgagctggt
    35161 aatgaaggtt aatggttttg ctccccccag ctctgagatt ttttcaccca cacctaagga
    35221 ttatgccaaa atcaaacttt tgaccggcaa aatgcagcaa aaatatgttg ctttaacacc
    35281 cccaaggcca tacattgttg ttaataccac ctataatagg tttaaactat ataagaatag
    35341 gaggttaatt cgtgagggat tctgcagcac tggaagctat acactcctca aatcgcactt
    35401 taatcgggaa tggatttttc aaacccccaa ggggttttac cgaattcaag gaaagataac
    35461 gaatcctgtt tggcggaggc cggactggtc gtttgtggaa gaggggcttc caattccttc
    35521 ccagaacgat ccttcccgat gggaagcagg agttttaggc gattacgcca tgagcattgg
    35581 tgatggctac ctcattcacg gaactatcta taagcgtttt cttggaatgc ctgttacgca
    35641 tgggtgcatc cgaatgaacg atgatgacct ggaagccgta tataaaaccc tcgaactagg
    35701 ctcaaaggtt tatatctact aatagctgat ctaatgcaaa aaaaattcct taaaattttt
    35761 cttctggctt gccttcccat cttcgctttg gccctggttt actttatact aaaattggtt
    35821 gctgttaacg gagcggttga tcgcttccaa aaattttact cgcagctaga tggagatgat
    35881 aagagtaatg ctgtttttca gcttaccaac gattctgcct ataatgctga gcagaacagc
    35941 tacgcctact accatgccca acttgaggcg gcaaattccg attccgttgt gctttgcatt
    36001 aacttcccgg atagcactgc taccctttta atcaagggtg ttacggttca taccgcaaaa
    36061 atcgaccgct atcgggtaag taattttttt actaaaatag atcaacgaat cctctacaaa
    36121 gctctttcat acccatttag ggttgaacgc caaacatcct caatcccaaa ggttccccta
    36181 atgattaaaa ttgcccccaa ggatacttca gaatatacgc ccgatatcgt ccccgatact
    36241 accgacgtta accctgtcta tttcttgctg gaagctaagc ccggcatgtt agtttatttt
    36301 tacgagactc caaaaaatca atcccggatt agcttcaacg gggtgattac tgatattaaa
    36361 gatagaactg ctattctgtt ggatgatcta cagagaatag tacacttccg aaagctggat
    36421 tacaagccat ctatcacgct aaggttgaac aggaaggatg caaaaattat cttcagggct
    36481 attcctaatg atgcccttat tattttaaag ctatcgtagg gggaaaaatg ccctgcaaac
    36541 ttgaaagcag gctttgtaac taatcaaaat ctttaagaga gaatttttta tcaggttgtt
    36601 tgatagtaac aatagctaag gaagaaaatt taaatcccct gagttcttgg tcggggtggt
    36661 gagattcgaa ctcacgacct ctacgtcccg aacgtagcgc gctaaccggg ctgcgctaca
    36721 ccccgattgt ttacctcttt agaacaggcc tactagaaaa ttgattgtga aaggttaatc
    36781 cttttttaaa tagctttaaa cccccaccaa tccatgcaaa ggtatttatt ttttgtatcc
    36841 taaatgcctt cggttaattt tttgcctgtt ttttttgtaa cttgttgcca taagaagcag
    36901 cttgatacta tggttgagtc ttttattcat ttcttatggc agtataaatc atttgccttt
    36961 tcgggcttaa atacttcaga caactgccag cttgaggtgt tagaccccgg tacatataat
    37021 tctgatgccg gtcccgattt ttttaatgca aaggttaggt tgaacggaac aacctgggta
    37081 ggaaatgttg agatgcacat taattcttcc gaatggtttg cccataacca ccacactgat
    37141 agagcctacg ataacgttgt gctgcacgtg gttgttaaca acgacaagcc ggctataaat
    37201 tcccgcggtg tgcctattcc cactcttgcc ataagctacc cgttcgggtt ggaaagagaa
    37261 tattcaagat tgacgagttc tggaagctac atcccatgcg gtgaggtgct accaaatgtt
    37321 gataacctaa caaaaagagc cctcctcgac agaatgatgg tggagcgttt acaggccaaa
    37381 accgatgaaa taggtaaatc gctcgaaaaa tgtcatggaa gctgggagga agctttctat
    37441 cgggcggtca ccagaacctt tggccttaag gtcaacgctt ttccggccga aatcttagcc
    37501 caggaaaccc ctttaaaggt gctggcaaag cataagaata acctttttca actcgaatcg
    37561 cttatatttg ggcaggccgg attactaaac cgttttgatg tagaggacga gtacctactt
    37621 tcgctcagga tggagtactc ctttcttaag aaaaaattta accttactcc gatcgatggt
    37681 agcttatgga aatttttacg aatgaggcct gtttcctttc ccactatccg ccttgcgcag
    37741 cttgccatgt taatacatca ttctacctct cttttttcca aagcggtgga ggcaagctcc
    37801 ttaaagcagc tgcaagaact tttccaagtt gaaacatcgc cttactggta taatcattac
    37861 ttatttggat ttccggttaa atcacgaaaa aaaagtttgg gcactgctac tatcaacgtg
    37921 attattatta atgccgtcat tcccttcatg tttgcctatg gtggttggag gggtaaccag
    37981 aaactaaagg aaaaggcaat ttcaattttg gaggagattc cccccgagga aaatcatatt
    38041 ataaaaaaat tcgattctat gggggttaag tcatgtaatt cgtctgatag ccaggctgtt
    38101 atacacttaa agtcaacatt ctgcgatgct cgaaagtgca tattttgccc tgttggcata
    38161 aaattattgc ttaaagcagc ctatatttag agtcttaaaa cgaacgtaaa aatacaccct
    38221 acaacaaact ttataacatt tgcggaaatc ggggaattgc tggagttaaa gaaaatacac
    38281 gtatttaatt gcaatatgag aatactggtt actggggcaa acggacttgt tgggaaaaaa
    38341 attacccggc agctactttc aggcggaaag catgaggttt atgctacatc cctaaaaaag
    38401 ctgcaaattc ccggcgctgt tacctttaca tcaaacctgc taaatgcaga cattaacttt
    38461 atggttgatg aaacccatcc cgatgttatt atacattgtg ctgcactttc cagtcccgat
    38521 gcatgtgaag ttgatcgttt tgcctgtaaa aggctaaatg tagatctcac cgcccgaata
    38581 gccatggctt gcaatgacta tggagttcaa cttattctga tgtccaccga ttttgttttt
    38641 gacggattga aaggtcaata tgcggaagag gatcgggcta atccaatctg ctactacggg
    38701 atgtcaaaag ctgaggccga gcaggtggta gcgtcaatgt cagcaccatg ggctatcgct
    38761 agaacctcac tggtattcgg atacgagcaa aggctgtccc ggtcaaacat tgttttacgt
    38821 gttgccgaga acttgataaa gggtaaccca tttagggttg ctttcgatca aattcgaacg
    38881 ccaacccttg ctgaggattt ggctcgaggt atcgaggcta tagctaccaa cagggcaaca
    38941 ggaatatttc accttgcagg aagcgagata atctcagtcg ctgatttggc ggctaggacg
    39001 gctgaagtat ttggattgaa tccagaactt cttttgccag tgagcactga aatgttaact
    39061 gagccagcca ggagacccct taacacttcg ttatgttgtc ataaggcaat tgagcagctg
    39121 ggctattccc ctaagggttt aaccgaggct ctaaggttgg tttactcgca aatgaaatta
    39181 gatgggtaca atccataatg cgcttgatgc gttgaatctc tggtagaaat ttgagcagcg
    39241 gtttcaagaa atttgcccat gcaaccttaa tataacactt gatacactca gccagcgtta
    39301 ttgccggttt ctaccatttt atatttccgg caattaaaac ctgaattttt tatttgtaaa
    39361 catgtatgca taccccctta gccggcatat cccatttgtt ctgttgagat ttgttaattt
    39421 tgtaagtttc tacctttatt accatgaagc acccatgtaa gaaagcttgc ctaagtatgt
    39481 ttgtacaagc gggggttacc tacgagctcg gcgaattcaa aatgtggatg tttttttcga
    39541 ccaaccctaa tgactagaag gtagggaata aagtatttat aatcagaaac ataaattttg
    39601 tattgatgaa aaaaatttcg gttaacattc agtcggaaat tggtgaactt gagggagtta
    39661 ttatccacac gccgggcccg gaagtagaga atatgacacc cgaaaatgcc gaacgagccc
    39721 tctatagcga cattctcaac cttagtgttg ctcgaaagga gcatgcccag cttagcggtg
    39781 ttctttcaaa agttactcaa acctttgagg ttggcgattt gttggaacaa gtgctgtcga
    39841 atccaactgc caaggaggaa ttgattagga gaatttgcct taccgagaat gccctgtggc
    39901 taatggacga tcttctggct gttagtccga aggagttggg aaaatttctt attgaggggg
    39961 ttccattggt aaggaataac ctgaccaact ttttgagcca cgaacgcttt tccctaaagc
    40021 ccctttacaa cctttacttc acccgcgacg cttcatttgc catatccaga agcgtagtca
    40081 tagccaaaat ggcaagcacg gttaggcatc gcgaaagcat aataatggag gcaatctttt
    40141 caatgtcgga tatttttgat acctcaacgg ttaatccttc tgcttttaca cttgcaagca
    40201 acgctaccat tgagggtggc gatgtgctgg ttgttaggga tgatgtttta ctcattggta
    40261 acggtgcccg aaccaacact catggtattg atttcatttt gaataggtta caattaaaca
    40321 gtgatgctag gaaaaagtat attcttgttc aggagctacc ctataagccc gagtccttta
    40381 ttcatctcga tatggttttt actttactgg atgcagataa gtgtatggtt tacgatccaa
    40441 ttattattca gcccaaccgg taccaaacag tccaaattac cattgcgaat ggaagggttg
    40501 aggaaataaa aacggttgac aacctgttgg ttgcgctaaa gaagttggga atggacctca
    40561 agccggttta ctgcggaggc accaccgata ggtgggttca ggagcgggag caatggcata
    40621 gcggggctaa ttttgtagcc atagggcccg gcaaggtaat tggctacgct cgcaacgtac
    40681 acaccattga ggagatgaac aaaaacgggt ttgaggttat tagggctaaa gatgtaattg
    40741 ctggaagggt tgatctaggc agttactcca gctatgtagt aaccatcgat ggaagcgaac
    40801 ttccacgagg tgggggtgga gccaggtgta tgacaatgcc tgtgcggcgc aaaaaagtta
    40861 actggtagga ttccgtttaa aaagcctgct tttcaaccat ctcctttacc aaattggctg
    40921 cttcctgaaa cgatgttgca gtatataccc ttaatcctga ctgatcgata agctttcggc
    40981 cttccaaggc atttgttcct tgaagcctta ctattaccgg aactgggata ttgcctattt
    41041 ccctgtaggc ctccacaact ccctgggcta ccctgtcgca acgaactatt ccgccaaaaa
    41101 tgtttatcag gataaccctt acgttgggat ccttaagtat gattctaaac cccgcagcaa
    41161 cggtttttgc attagcactg ccacccacat ccagaaaatt tgccggttcc cctcctgaaa
    41221 gtttaataat atccatagtt gccatggcca gcccagcccc attcaccatg cagccaacgt
    41281 taccatcgag tttaacaaag ttaaggttat tctttcctgc ttcaacttcg gcaggttcct
    41341 cctcgtcaag atcgcgttgc cctgctatat cgggatgacg gaaaagggcg ctatcatcaa
    41401 ctgtacattt ggcatcggct gcatatatat catcgtcgga tgttttgact acagggttta
    41461 tctcgagtag agaaaaatcg ttggcttcat aggtgttata aagattcgct aaaaatttca
    41521 ccatctggga gtatgctttt ccggttaggc caaggttaaa ggcaatttta cgggcttggt
    41581 atggttggag acccagaggg tttatttcct ccttgaatat gagttggggg ctttgtgagg
    41641 caactgcctc tatgtccatt cctccctggg gcgagtaaac caccatgttt cggctggttg
    41701 atctatccaa tagtatgctt atgtaaaact cctttacctc cgctggccct ggctggtaga
    41761 tgccctgttc taccaatacc tttccaacca gtttgccctt aggccctgtt tgatgcgtaa
    41821 ctagcctcat cccaatgatg tcgtgtgcat atcttcttac ctcctcggtt gatttggcaa
    41881 tctttacccc acctccctta cctcttccgc ctgcatgtat ttgggcctta actgctataa
    41941 catctgttcc tgtggcttct atcacacttt cagctacctt aacagcttcg tcgggcgtaa
    42001 atgccacact acccaatggt acattaactc ccgatttctt tagtaatgcc ttagactggt
    42061 actcgtgtag gttcatactc ggctttctat tttgatttaa gttgttaaat ataaaaaaaa
    42121 tgattgagta attttgtcga aaattcaact tatggctaaa aacagaaaac tttccaacga
    42181 agagcttcac aggaaaactg ttgacgagta taaaatggtt gaaaagtttc ctattgtcgt
    42241 tgttctggac aatgtcagga gcctaaataa tattgggtcg gtttttagaa cttccgatgc
    42301 cttgatggtt gagagggtag ccctttgtgg aatatgcgga aaaccaccac atcccgaaat
    42361 tcatcgtact gctcttggag ccgaaaattc tgttttatgg gagtattttg ataaaactga
    42421 aatggctatt gagaagttga gaggggaggg ataccgcatc atctcttttg agcaaaccga
    42481 gcattctgtc aacttagttg actatatgcc catgccagga gtgaaatatg ctttagtatt
    42541 tggcaatgag cttcgtgggg tagggcagga tattgttgac ctttcagatg aatgcgtaga
    42601 gataccgcag tttggcacca agcattcgtt caacatttct ataactgctg ccatagttct
    42661 ttgggacttc ttcgctaagc ttaagaagca ttaaggcaga gcggcaaacc aacaaccgta
    42721 gcaccaataa aaaaacctgc caacggcagg ttcttttata aactttttac tgttcagcta
    42781 ttcagcaata gccttgaaag cttcgttctg gaagaagtta cgaaattcaa catcatcctt
    42841 tgcacgagcc ttcaacgagg catccttgcc aatagcggtg cgtaggttat caagtgtggt
    42901 agcttcctcc tgcatgcggg cacctacaat agcacggcca taataaccct tagctgtttc
    42961 aacctttgca aaagtattct ttgctgcatc aacctttccg ctaagaagca gggcaagtcc
    43021 ctggttgaac gagtcagttc cgccaaggta ttcagcagca gcaggatact gacctttaag
    43081 aatggctatt ataccaaggt tgtacttaac ctcgttaccg gcgtcggcag catcagcaaa
    43141 tagtttttca gcagcttcat agttcccctc catcagcgca atgcaaccac ggttgttggt
    43201 aacggcagga attttctgat cggcaggaat tttatccagg gcagcctttg cagcagcagt
    43261 gttcttgttg aggatatagt agtatgcagc attgttataa ccgcgagcgc agttgaactt
    43321 ttcgcctgcc ttcatgtaaa cctcaatctt tttatcaacg ttatctacaa gggttgctgc
    43381 gtaaagcatt tcctcaacat taagtgaatc gtaagcattg ttctcaacca tatactttaa
    43441 cgtatcgtca gagtagccta ccttgtcaa
//
LOCUS       DAOPHX010000005        40318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_896_length_40318_cov_6.685468, whole genome shotgun sequence.
ACCESSION   DAOPHX010000005 DAOPHX010000000
VERSION     DAOPHX010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 40318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40318
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_896_length_40318_cov_6.685468"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..222)
                     /locus_tag="PK502_00875"
     CDS             complement(31..222)
                     /locus_tag="PK502_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99399.1"
                     /translation="MKYSLSGRSFRAGCSITLQALHRTYSRSAKFVASSFINRGLLTA
                     DKKITLPHFTVKLHTYYNP"
     gene            complement(316..1281)
                     /locus_tag="PK502_00880"
     CDS             complement(316..1281)
                     /locus_tag="PK502_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPG99400.1"
                     /translation="MKNILVVGAGGQIGSELVPYLRKIYGNEHVVAADINSSACTHLA
                     EEGPFEELDALKADRYLEIVKKYRIDTIFNLVALLSATGEKKPQLAWHVNMGALMNSL
                     EIARENSCAVFTPSSIGAFGPTSPLDNTPQDTVMRPTTIYGICKVTGELLSDYYFLKY
                     GVDTRSVRFPGIISNVTLPGGGTTDYAVEIFYGAVQHKHYVCPLPEGTYLDMMYMPDA
                     LDACVQLMEADPSKLKHRNSFNVTAMSFEPSQVYSAIRRRIPDFTMEYSVDPVKKAIA
                     DSWPNYMDDSCARQEWGWSPKWNLETMTDDMLRAIGEKMKKGLLG"
     gene            complement(1731..2711)
                     /gene="floA"
                     /locus_tag="PK502_00885"
     CDS             complement(1731..2711)
                     /gene="floA"
                     /locus_tag="PK502_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137107.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HPG99401.1"
                     /translation="MSQVGAILTIIAVCIVGLWIIFYFVPVGLWFQALVSGVRISLLQ
                     LIFMRWRKVPPRVIVQAMIEGTKAGLTLNRNEMEAHYLAGGRVPKVVHALVSAQKANI
                     PLDFKMATAIDLAGRDVFEAVQMSVNPKVINTPPVTAVAKDGIQLITKARVTVRANIK
                     QLVGGAGEETVLARVGEGIVTAIGSSQNHKEVLENPDKISKTVLNKGLDAGTAFEILS
                     IDIADIDIGRNIGAVLQMDQANADKNIAQAKAEERRAMAVALEQEMKAKAQEARAKVI
                     EAEAQIPLAMADAFKSGNLGIMDYYRIKNIEADTNMRDSIAKPGVEPKKK"
     gene            complement(2717..3178)
                     /locus_tag="PK502_00890"
     CDS             complement(2717..3178)
                     /locus_tag="PK502_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPG99402.1"
                     /translation="MIWIFLLILLGLFLLVAEVLLVPGITVAGIAGFLLIGYGVFAAY
                     TEHGTIAGHITLVFSIVASVITIVLSLRSKTWKKITLNTSIDSKANMDYEQHIQPGDT
                     GVTIGRLAPMGKATINGHDVEVTALDELVDPQTTVVVVKVEGSKIIVKPKK"
     gene            complement(3195..3476)
                     /locus_tag="PK502_00895"
     CDS             complement(3195..3476)
                     /locus_tag="PK502_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HPG99403.1"
                     /translation="MKVKEKTFGLEHTQNKSSEVYTLFLHNDDVNTFEFVIQALVEVC
                     RQDENQAEQCALITHFKGKCDIKRGSLPELEPMMEELHRRGLSASINLN"
     gene            complement(3616..6399)
                     /locus_tag="PK502_00900"
     CDS             complement(3616..6399)
                     /locus_tag="PK502_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99404.1"
                     /translation="MRLTRITAILFFSCIALAANGQEIGMTGTKANAERVMADSLLKN
                     QQYAQALPIYSRLLEQHSRDPQIQLNLGICYLYGTRRMDLAVKFLSQASTGDVSSIVY
                     FYLAEALRQSYRFSEAIDYYRRFTISGGASDIKLEEVEQLVTLCENGSFLTRYIYNPE
                     VYDTKAVAAGEACTFYSIAPKSGSFVNAPTNLLTPTDIKENHQPLMFYPTNPTPGTYV
                     YYSSYGSTTTYGKDIFRIQLQDNGSWSKPENLGDVVNSNFDEDYPYIIPDGVTLYFAS
                     KGHYGMGGYDIFRTVFDPTTKRWSPPENLAFPINSPFDDFLFVADDSDSLACFATNRK
                     VMPDTVEVILMRNEQNPIRRSPRSYSELDSIAALKVSTLALNAPTKNPTTDQAEKPIA
                     ASQKPASFNSVENDPEYSRTIAKGFAAQQLADSLKIKLEGLRGKFDYVTTAEQRIRLE
                     KQVVQVEDDMLAAQKEADLMFVRASQIEQEYLTGKRKPKGDESLSFVSDNPDYIYQAQ
                     FAPTVFQTDELNRLAQAEQLYPQVLRNRNLALTNRDIYDQCKKNSTDSSTKVCDTKYA
                     DMLSSMQSYNEQMGKYYEKKYPIYNDCVHVALVKSGSNDQSIRKCISTAYSHFRAAGT
                     ILNNMGNDGRVESAFEANLLRELGLLQLDLAFAKIWHLQLFEQKLTSSIIKFERSAFG
                     STSAIELLSAEQSKPQQAAASSYTPPRIERVSSPVNIENLAVESEISSDFGISKTSPY
                     NEENPIPAVPALPNGICYKIQLGVYGTPRQPQFFKGIYPISAEPVGKLTRYYAGVFAR
                     LADAESALSKVKSVGFRDAFIVAWYNGQQVSLQRAQQLEGSLQQQKSSDSDRKGVFAI
                     QLGVYEGSMPNDELKTIRTLIPNKDITRRVDERGLMVYSIGNFSTLSEAERNKDNLIA
                     GGILNAKVIELNQ"
     gene            6374..6721
                     /locus_tag="PK502_00905"
     CDS             6374..6721
                     /locus_tag="PK502_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99405.1"
                     /translation="MAVILVNRIGWIRIFTKIKKHSKLHEKVDKVESCRVVMGNGLKM
                     THPKLHEISIYSCTGDLCFWLQNSAVRPIPNIGFSSIVNGIKCKDFPTIVARNFERLY
                     LCGISYGKKIEEA"
     gene            6732..7412
                     /locus_tag="PK502_00910"
     CDS             6732..7412
                     /locus_tag="PK502_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HPG99406.1"
                     /translation="MPVAVFLSAALMLGIIRFISKPDHRYVIRGIDVSAHTGSISWEK
                     LKDQRVYFAFVKASEGATLKDKNFQDNVNGARAAGIPVGAYHFFNFNRDGLTQANNFL
                     STIGRQKYNLPLVVDVEEWGNNIRRSRSQIIADLREFVSKVEKNRYQKIIIYTNEDCY
                     KLYIQDVFPDNDIWICSFNHKPKLNREWTFWQYEHNGRLKGVKGKVDFNVFRGSMLEW
                     RQYIEDGR"
     gene            7594..8820
                     /gene="clpX"
                     /locus_tag="PK502_00915"
     CDS             7594..8820
                     /gene="clpX"
                     /locus_tag="PK502_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPG99407.1"
                     /translation="MVKNTDLCSFCGRSRKEVSLLIAGMDGFICDLCVEQASIIVSEE
                     LKKKGEFKLQKSKLMRPTEIKKFLDQYVIGQDEAKKYLSVAVYNHYKRLLQKDMGDDV
                     EIEKSNILLVGETGTGKTLLARTIARMLHVPFTIVDATVLTEAGYVGEDVESILTRLL
                     QVADYNVEAAEKGIVFIDEIDKIARKGDNPSITRDVSGEGVQQALLKLLEGSVVNVPP
                     QGGRKHPEQKMIAVDTKNILFICGGAFEGIEKKIAQRLNTTVVGYGAGKTREVIDKNN
                     LLQYIAPQDLRAYGLIPEIIGRLPVLTFLSPLDKVALRKILTEPKNAIIKQYIKLFEY
                     DGIKLQFEEEVLDYIVNKAVEFKLGARGLRSICEAIMMDAMFDLPTGSEKEITVNLDY
                     AQQKLEKSNLKRLKAA"
     gene            complement(8817..9302)
                     /locus_tag="PK502_00920"
     CDS             complement(8817..9302)
                     /locus_tag="PK502_00920"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPG99408.1"
                     /translation="MVISIVVATSENRVIGKNNQLLWHISEDLKRFKKLTTGHTVIMG
                     RKTFESIGQPLPNRHNIVISRSEGLKFEGCTAAKSLADALEIASTDEEVFIIGGGEVY
                     RQALPLAERIYLTLVHANYEGDTFFPALNPNEWKVVREEKVSPTEGPGISFLDYVRQH
                     S"
     gene            9503..10792
                     /gene="purD"
                     /locus_tag="PK502_00925"
     CDS             9503..10792
                     /gene="purD"
                     /locus_tag="PK502_00925"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPG99409.1"
                     /translation="MSGVNVLIIGSGGREHALAWKLSQSPKLGKLFALPGNPGTARLG
                     ENIPLSVLDFEGIRTVVLEKNINLVVIGPEEPLVKGLYNFLTGDLGLRNVLVVGPGRE
                     GAMLEGSKNFAKAFMQRHGIPTARYRAFTRDEYSEAKKFLGTLSSPYVLKADGLAAGK
                     GVVIQASYEDACASLGEFFSGKFGDAGNVVVIEEYLRGVELSVFVLTDGKSYVILPEA
                     KDYKRVGAGDTGPNTGGMGAVSPVPFATPEFMKKVEETIVKPTISGLASEGIPYCGFI
                     FFGLMNVVGNPFVVEYNARLGDPETQVVLPRIKSDMLELLMLCAAGKMNEAELVVAPQ
                     FAATVILASGGYPGSYEKGKAISGLEASANAIIFHAGTTSSGSMTLTAGGRVLACTGT
                     GESMEDALASSYCAANAITFESKYFRSDIGMDVYPKS"
     gene            10809..11816
                     /locus_tag="PK502_00930"
     CDS             10809..11816
                     /locus_tag="PK502_00930"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HPG99410.1"
                     /translation="MLLKFFRRFRISTLLVMLGVITLFWAGTLMQKYPPSTFTFDSFG
                     MPLYVLIVKILSPYSFLSKLVAFALTLTVAIYLLQFNVRHTVVRQRTYLPFLFFVLVA
                     SSLTPLQRLNPAIIANIFILVSLSHVISTYQNSEALDHLFRSGLLLGIGTLLYAPTVV
                     FVFLIFWSIATLRQFNLREWLVTLVGILVPWGAVVFYFYWFDLGASEPFIQLANSLCL
                     EAESSADKIAPIILCVAVGVPLLVAILHMLTGMGSQKIAIRKHQNIMLLFTVVALLSF
                     FVVPSCSYEILYIAAAPISLLLSEYFSDAKGKYWPEILFLIFILGLVSMQLYPLLMHS
                     L"
     gene            complement(11927..12853)
                     /locus_tag="PK502_00935"
     CDS             complement(11927..12853)
                     /locus_tag="PK502_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HPG99411.1"
                     /translation="MKSFWKRDLGLVPSEEWEPYCWVDVEIPTEEERKYLFDELGVPE
                     AFFNDIEDVDERPRIEFEDEWFFILLRMPYKNVDVSLPFTTIPVGIIFKDDIFVSISF
                     YRTEVIPDFIQFTIRKGIVIKDHFDLVLRLMLSSSVWFLKYLKQINLDIRLAEDQLEK
                     SIKNEDLQTLLSIEKCLVYFTTSLKGNDILLHRIKNLRNYKDTYNPDLIEDVEIELRQ
                     AQETTSVHSDILSGMMDAYASVISNNLNNVMRQLTSISIILMIPTLIASIYGMNVPNG
                     LENNPWGFGLVVLASFLFSILGIVLFRRKKWF"
     gene            complement(13187..14314)
                     /locus_tag="PK502_00940"
     CDS             complement(13187..14314)
                     /locus_tag="PK502_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPG99412.1"
                     /translation="MEELVRLRHELHRNPELSGHESATAKRLRFFLSRYNPDELYSNI
                     AGEGMAVIYQGKEPGPTVLFRCDMDALPIAETNDVKYKSNVQGVSHVCGHDGHMTIIS
                     GLAVRFSQSKPKKGRVILFYQPSEENGEGARKSLEGLKELGLNPDYAIALHNLPGYKM
                     GSIILGRYTFSAASKGLVINLKGKPSHAAYPEHGLNPAAATAEIIQSLLSIQSTTHFK
                     NFILVTVIHIRVGDIAFGTSPGNATIMATLRAFDDDDMTLLSKIAAETATSIAHKHKL
                     GIDVSFTDEYPASVCDKTLTHTLEAIAHEASLEVNFLEQPNRWSEDFAHFSTCFPSVI
                     FGLGAGENHPDIHTPEYDFPDELIDNAVDFLGKIANKLMEG"
     gene            complement(14500..15003)
                     /locus_tag="PK502_00945"
     CDS             complement(14500..15003)
                     /locus_tag="PK502_00945"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HPG99413.1"
                     /translation="MAGTIDHPIKKVLIICAKANIEDVYAALVMANGAVMEGIETKMF
                     FTFFGLDAITKKSMKKLHTSAVGNPGLKMPGGLPFPTLLGILPGVEAGVSAMMKSQME
                     KLDIPTVEEFLELITAGGGEIYACKLAVDMFGLKKEDFSEHVKDIITVGQLYELAAGE
                     GSQIIFT"
     gene            complement(15021..15332)
                     /locus_tag="PK502_00950"
     CDS             complement(15021..15332)
                     /locus_tag="PK502_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HPG99414.1"
                     /translation="MSEKVYAGKSVTVTEDGYLTDPNQWTKDIAVEIAREEGITLSPK
                     HFEVIEFIRDKTLKGEVMTIRTIGKSGIIDTKGFYELFPGAPLKKASRIAGVSKPTSC
                     V"
     gene            complement(15350..16024)
                     /locus_tag="PK502_00955"
     CDS             complement(15350..16024)
                     /locus_tag="PK502_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99415.1"
                     /translation="MDERIIEQQIDALNQKLDTVIELLVSQHQKNEVVEDLIDDLSIV
                     GKNAFQATVDELAVQNISIEADDLKYLFFKVLGNIKTFGELMDMLESVVDLVKDLGPI
                     VHDASIAVTEKLGQLDREGYFSYLNQLGLLAIEFKKNFTEKDITRLREEMPAVGAIVR
                     NLSQPQVVNALATITETLSEMKLDDKLDNKSLFRLFRELNKPDVRKTLSFTLRLLAEL
                     GKKQDV"
     gene            complement(16036..17265)
                     /locus_tag="PK502_00960"
     CDS             complement(16036..17265)
                     /locus_tag="PK502_00960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HPG99416.1"
                     /translation="MKRIVILGAGTGGTIMANKLRKALDREQWEITIVDKDPVHYYQP
                     GLLFIPFGIYGKQDVVKPKASFIPAGVNFIINEIERVDPAKNRVYLANGQTLDYDFMI
                     VSTGTSIRPEETPGLLGNEWGKSIFDFYTFNGAVALQNFLKSWEGGNLVLNIAEMPYK
                     CPVAPLEFVFLADEFFVKKGIRDKVNIFYSTPLSGAFTKPKATKMLGDLLQEKNINLI
                     PDFYLERVDAENKKIISYDGRELPFDLLTIIPVNKGADFIERSSMGDDLNYISTDKYT
                     LRSEKWSNIFVLGDASNLPTSKAGSVAHFSSDVVFDNLISSIEGRPLMAKFDGHSNCY
                     IETGFGKGSLIDFNYDTEPLPGTYPVPGIGPFGLLKNTRINHYGKVMFRWMYWHLLLK
                     GKELPVDSQMSLIGKEL"
     gene            17681..18379
                     /locus_tag="PK502_00965"
     CDS             17681..18379
                     /locus_tag="PK502_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPG99417.1"
                     /translation="MKAEVCPGTECLTCGFRSEVFASLPNYQLEELSRSKVLMHAKKG
                     ETIARQNDSIEGFIFLRDGLAKLCRTNAEGREQIVGIATPNDFVGLLSLFSSKTYNYS
                     IVAIEDSSYCSVEYDMVMNLVGSNGEFARNLLAKISRVSDLLIGSRLDIDLLQLRGRV
                     AYILCFFANEIYHSTRFNLPVSRREIAELIDMRVENVVRILSEFRRDRIILIEGTYIE
                     ILDKEKLSWAKMHG"
     gene            complement(18387..19136)
                     /locus_tag="PK502_00970"
     CDS             complement(18387..19136)
                     /locus_tag="PK502_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG99418.1"
                     /translation="MITVKNLSKSFDGRMVIDNITATFEKGKTNMVIGQSGSGKTVLL
                     KCIVGLLDVDEGEIYYNDIQFNALSFKKKKEIRQKIGMLFQGAALFDSQTVEENVMFP
                     LNMFTNMAIAEKKERVAFCLKRVDIVGANKLYPAEISGGMKKRVAIARAIVLNPEFLF
                     CDEPNSGLDPKTAVVIDHLIKEITEEYGITTIINTHDMNSVMGCGDKVMFIYNGKKWW
                     EGTRFDVLDSDNQELNDFVFASALTRKIKGM"
     gene            complement(19133..19876)
                     /locus_tag="PK502_00975"
     CDS             complement(19133..19876)
                     /locus_tag="PK502_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG99419.1"
                     /translation="MVRIFGLIGEYFLLLKKVFSKPEKGRIYLKQTIKEMDKLGIQSI
                     GIVTIISVFMGAVITLQTAYNMENPFIPRYMIGLGTRDSMFLEFSSTIIGLILAGKVG
                     SSIASELGTMRVTEQIDALEIMGVNSASYLILPKIIATLIFNPFLCMLSMVVGIAGGW
                     MVGEFSGVISTQAYIYGIRYAFIPFYITYSLIKTVVFAFLITTISAFYGYRVQGGSLE
                     VGQASTNAVVVSSVFILLFNLILTQLLLS"
     gene            20203..21294
                     /locus_tag="PK502_00980"
     CDS             20203..21294
                     /locus_tag="PK502_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPG99420.1"
                     /translation="MSNKNYYCIVMAGGIGSRFWPLSRTACPKQFIDILGVGKSLLQQ
                     TFERMVKICPVENIFIVASNSYKNLILEQLPEVKESQLLLEPMRRNTAPCIAYATYKI
                     RKRNPEATVIVSPSDHLILNEQVFTNTVNLALDFASKNNALLTLGVKPNRPETGYGYI
                     QVGKPVKGINGLYRVKTFTEKPNLEMAKVFLSSGEFFWNSGLFIWNLNAITQALEEHL
                     PEVNSLFSEVASLLDSADETAAIEKTYAECKNISIDYGVMEKAENVFVICSEFGWSDL
                     GTWGSLYTHLPVNQNGNAVIARNVLLYDTSNSLISAPDGKLAVVQGLDDYIVVDTSDV
                     LLICRKQEEQQIKNILNDVAMLKDGEIYT"
     gene            complement(21375..22256)
                     /gene="gldN"
                     /locus_tag="PK502_00985"
     CDS             complement(21375..22256)
                     /gene="gldN"
                     /locus_tag="PK502_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HPG99421.1"
                     /translation="MKRIVVLFGGFFLILGTLHAPAQDRKESLVNDGFYTRETIQQRK
                     PIPYQYVRESDVLWSKRIWRMVDLRQKMNYPLYFPTTPMQDRESLVQRLVRAIKYNEI
                     TAYDPVDDEFTTRMSYEQVIKNLGAEDRVEKQIDENGNEVEVTIKGEIKWSGIKELLI
                     KEDWFFDKQTSTLQVRIVGICPIMHDMRILQTGEEDQEETGEITKKQTFWVYFPEART
                     ILAKTDFFNSFNDAQRISFDDLFFKRQFDSYIIRESNVFDNRLISDYTFGGVQTMQES
                     EKIKTDLFNMEHDLWEY"
     gene            complement(22349..23986)
                     /gene="gldM"
                     /locus_tag="PK502_00990"
     CDS             complement(22349..23986)
                     /gene="gldM"
                     /locus_tag="PK502_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HPG99422.1"
                     /translation="MAHGKETPRQKMIGMMYLVLTAMLALNVSTDVLEAFVLVDNGLS
                     RTTENYRLKNDKLYSEFDKAMLVNPVKVGPWKSKADEIKEMAREIYDYVQDLKIEVVK
                     TSEGDDTPAINGREVISELIEDKANLDIGGQILIGMEGNGKAFELKRKIIKFREKAIS
                     LLDPVRGEEMIKSINSILNTDDPPVREDGTHNSWESSRFEHIPLISVIPQLTKVQVDI
                     LNTEAEVVNYLLQQIGASDFKFNVLSATVIPNSNVVFQGSDFKAEVFLAASDTTQKPK
                     IYLCRYDSTQNPVTKEWDYNPIGPQEEIPVSQNGRGLYSRRAGALGQTSWSGLIEMKA
                     PDGSIVRKPFKHTYSVLQPNVVIAPTKMNVLYLGVDNPVDISIPGVPPDKVTASISKG
                     SIRPEGKGSYIVQPGAGASTCEITVYANLEGGRQSMGTKTFRIKKVPPPLPRIIGITG
                     KYAEKNQLAASLGIAAEMPQDFDFDLKYKIVSFRLSATVGGFLQEKESNSSRFTDEQK
                     KIVSNLRSGSQLAITDIKAVGPSGDVIDLNDMVLKIR"
     gene            complement(24020..25306)
                     /gene="gldL"
                     /locus_tag="PK502_00995"
     CDS             complement(24020..25306)
                     /gene="gldL"
                     /locus_tag="PK502_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HPG99423.1"
                     /translation="MKINIEELVTSRKWKTFMKYLYGWGASVVILGALFKILHLPGAG
                     TMLMAGMCTESIIFFFSAFEPLPDEFDWTLVFPELTGMTEEDEIDGYKSSRRQGIGAE
                     EIHEIVTGILASMPAGSVPAAQAIAPEKVETTPPHQAAATAAPQISGQHAGAGAGGAL
                     IFTEKFNQMLENAEISPALFDKVSQGLNKLSQASSSIADITTTAVHVKEFTDNMAKAS
                     ESVKTFHENYSQNSQVLNESMNHLSDSIQKTAGEMSESGKNFMDGVAGSVQNLENELT
                     AAGQKVGGRIVESCETVAGKLNMAAEGLANAYQQTAEGVAITYQQLAEAMKNNGSTIT
                     KGSTTYNQQLEQLNKNMAALNTVHEMHLQETSQRLKEAEKLYQGVDGMIKKLNVTVDE
                     SEKFKSALETLNSNITSLNNVYGNMLAAINSISTSK"
     gene            complement(25347..26675)
                     /locus_tag="PK502_01000"
     CDS             complement(25347..26675)
                     /locus_tag="PK502_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPG99424.1"
                     /translation="MKKLLPIGIAVILLSSCSPGGNGELTGVPGRRGFSEPTPYGMVF
                     IPMGSFTIGSNDQDVAWAINSPTKTVSVDAFWMDQTEITNNQYRQFVYYVRDSIARRM
                     LAEVNDEFVITEDEFGNPIDPPVLNWEVPIDPNDEEQMEVLNNMYYSKEDAFYGRKEI
                     DTRKLFYEYYWIDMKQAGQKNNRYNFETLSYDKGEVVNSLGEKVKVDNRQSFIMRDMV
                     NVYPDTLCWISDFSYAFNEPYANSYFWHPAFDNYPVVGVTWKQATAFCIWRTQLLNKY
                     LAPEGITIQDYRLPSEAEWEYAARGDLDHSMYPWGGPYTRNIEGCFIANFKPLRGNYI
                     DDGGLIPIAAGSYEPNDYGLYDMAGNVAEWTANAFDESTYSFMHDMNPDYKYNAKPND
                     LPAMKRKVIRGGSWKDISFFLQVGSRAFEYQDSAKSYIGFRCVRSYLGAK"
     gene            complement(26732..27676)
                     /locus_tag="PK502_01005"
     CDS             complement(26732..27676)
                     /locus_tag="PK502_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPG99425.1"
                     /translation="MHSSSRKFLLFQLFLPFAGILMAQQDPQFSQILYNPMSINPGYA
                     GSQEMIGIGAFNKLQWTGFGDGAPVSTTVSINAPIAPFGFKSGLGLSILNDQYGFNSD
                     LGISLAYAARFKPKLINGSLGIGVSGGFVNNTIDPKWNYPVAGNDQAIPQQKESSVNL
                     DLGLGLFYTTDYMFFGVSATHINRAKINKSPIPARYTPQYYISGGYIVMFPSKTWQFE
                     PSVLIVTDIKSSRLSLNSIVRYNKKFWGGVSYRINEAVTGMIGFELFNGFRIGYAYDF
                     SLSRISKYNDGSHEFMLGYSFQLKKEKPPQQYKSIRFL"
     gene            complement(27916..29088)
                     /locus_tag="PK502_01010"
     CDS             complement(27916..29088)
                     /locus_tag="PK502_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HPG99426.1"
                     /translation="MDELLSFLTPTAIKGVNFSSQNSRMLQQQVVPLNKTNYKHLKKN
                     DIVVIGVNESRNSPNPSIKNSPETIRKYLYGLSTIKLAGKIYDAGDLVETSTPLKTYE
                     ALNRVVEILFQTGAATVVLGGTQELTYCLYEGVKSNKSLVTISIIDCLIDADEDLSDF
                     HSRNYVNRLLGEKLRSLFELSIVGYQGYYVGGDMVNLFQKNNHELLRLGYVRSSYREV
                     EPTLRNADIVSFDMEAIRACDSPANAIPSPNGLYAEEACQLAHYAGVGERASLFGIFN
                     YNPDSDTTKQSALLAAQLAWHFIEGYSKRKNVPKTLNTPDYLKFIVGTGTSGVDMVFY
                     CDEALDRWWMEIPISPEMGIPTNRIIIPCSHSDYTLASRQELPDRWLRIFQKIQQL"
     gene            complement(29125..31479)
                     /gene="topA"
                     /locus_tag="PK502_01015"
     CDS             complement(29125..31479)
                     /gene="topA"
                     /locus_tag="PK502_01015"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPG99427.1"
                     /translation="MIQNLVIVESPAKAKTIEKFLGKEYVVKSSFGHIRDLSKKNLGV
                     DIAKNYAPEYIVSDDKKKVVDELKKATSEAQMVWLASDEDREGEAIAWHLAEVLNLNP
                     KKTKRIVFHEITKSAILNAIETPRSIDLDLVNSQQARRVLDRLVGFELSPILWKKVKP
                     ALSAGRVQSVAVRLIVEREREIIAFTPTPYYRVTAIFMADSEFNAELSVRLSTFEEAL
                     SLLEHCKNATFTVQDVTKKPSKKAPAPPFTTSTLQQEASRKYGFSVAQTMSIAQKLYE
                     AGHITYMRTDSVNLSSTAIAAARDVIVAEYGEKYHQPRQYKTKTKGAQEAHEAIRPTY
                     LDRVKISGTSTEQKLYDLIRKRTIASQMSDAQIEKTTINIDISGSHYKFVASGEVIVF
                     DGFLKVYLESSDDEAEENGREILPPVKAGQTLAVMEIVANERFTQRPPRYTEASLVKK
                     LEELGIGRPSTYAPTISTIISRGYVAKEDRPGVERNYKSIILKNGKITRQEKVENSGA
                     EKAKLFPSDIGMVVTDFLLEYFRDILSYDFTAHIEEKFDEVALGKLEWTRMIDEFYKP
                     FHSLVEKTIKESDYTKGERVLGIDPVSGKPVSVRIGRYGPVVQLGEADASKKTKPQFA
                     SLLKGQLIETITLEEALSLFKLPRSLGNYENSEVVVGVGRFGPYVRHQSKFYSLKKDD
                     NPLTIDLNRAVELIEEGRKKERERVIKKFESPQVVEILNGRWGPYISFNKQNYKIPKD
                     KDPKLLTFDDCMKLIDEQAEKGSKRNSTAKKTLPKRQGKKGNTK"
     gene            complement(31841..33103)
                     /locus_tag="PK502_01020"
     CDS             complement(31841..33103)
                     /locus_tag="PK502_01020"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPG99428.1"
                     /translation="MNKKKVIIAGGGFAGIQLARKLDQRLFDVLLVDKINHHQFQPLF
                     YQVATSQLEPSSISFPLRNIFSRKKNVQIRLAEILSIDRNESKIITSIGEFSYDYLVL
                     AMGCTNNFFGNENIKKNAFTLKTTYDAITIRNHIILTFEKIVSAKEDELEALNNLAIV
                     GAGPTGVELAGAFAEIKKNILPKDYAGVDFSKFNIILIEGTRNTLGSMSDASKVESRR
                     YLQQMGVTILTETFVADYDGYLLKLSNGETIKTKTVIWVAGVTGNNIEGLPEAARAPG
                     NRISVDRFNRVNGCDNVFAVGDIAYMVTPKYPKGHPQVANVAINQAKLLAKNLKSIQT
                     GKKLKAYEYKNLGTMATIGRNKAVVDLPHIHLKGYFAWFIWMSLHLMLILSVRNKLII
                     FINWAWSYLTKDTSLRLILKEDKPRTNT"
     gene            complement(33535..35082)
                     /locus_tag="PK502_01025"
     CDS             complement(33535..35082)
                     /locus_tag="PK502_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HPG99429.1"
                     /translation="MKTKLLCFILLLAVIPVLGQVTPQAPQKFSYQAVLRNADGTVIG
                     NQSVEVQVKLHAQAADGAVVYTQTQTVETNQFGLVAFAVGGSSGFETIPWTDPIFMEI
                     LVKRSGESSFSSMGTTQIISVPYALVANNGIQGPYIQGQTVFSNGGGWVGTNRVMVAD
                     SSVVVTTPLGHDSSKPIFAVTNANGEMVMAVYESGVRFYVEEETTKGVKGGFAVGGLT
                     NQVKETKSGEPIYYMNIQPTQVQFLIEEPTEKGVKGGFAVGGLTNQGKTLTPTENYFE
                     VNRDSAQFLTPSLVSQGNITTQGNITVSGNITTNVGQADSTLTDVDGNTYQTVRIGFQ
                     QWMAENLKTTHYSDGTPIDTAVHVYNDWAYPDSINIYGRLYSGLAISSGNNVCPDGWH
                     VPTPDDWNELFTFIAGPAGISDMQSRVLVLKKLLEPGTMMEGNGYWNYPIYPTNETGF
                     TARPAGQRDYLTYYSELHDYAYFWSYVVGVGLQQVSMTTMDGGNLEIMPPSDSNYSFS
                     VRCLQNP"
     gene            35551..37353
                     /gene="typA"
                     /locus_tag="PK502_01030"
     CDS             35551..37353
                     /gene="typA"
                     /locus_tag="PK502_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPG99430.1"
                     /translation="MQKIRNIAIIAHVDHGKTTLVDKMIFQGKLFKDYERPGELILDS
                     NDLERERGITILAKNLSVNYKGYKINIIDTPGHSDFGGEVERVLNMADGVLLLVDAFE
                     GTMPQTRFVLQKALAIGLKPIVVINKVDKPNCRPAEVQEQVFELMYNLDATEEQLNFP
                     TIYGSAKQGWMSNDWQKPSKDITPLLDCIIDYVPAPKVTEGTTQLLVTSLDYSSYVGR
                     IAIGKLHRGSLQAGQPISLVKRNGEVVRTKIKDLLVFEGLEKNSVERVECGDICALVG
                     IEGFDIGDTIADYENPEPLPTIAIDEPTMSMLFTINNSPFFGRDGKFVTSRHLKERLD
                     RELEKNLALRVEKTDSADSFMVFGRGVLHLSVLIETMRREGYELQVGQPKVIIKMVGG
                     EKCEPIEYLTIDLPEDVSGKAIEQVTRRKGALLLMERRGDRIHLEFEIPSRGIIGLRS
                     ILLTATAGEAVIAHRVKGFEPYRGDFETRTNGSLVAMESGISYAYALNKLQDRGRFFI
                     DDHEEVYAGQVVGEHNRSGDLVVNVTKSKKLTNMRAAGSDEKIFLAPPIRFSLEEALE
                     YIQEDEYVEVTPKAMRLRKIILDETQRKRIARVA"
     gene            37468..37992
                     /locus_tag="PK502_01035"
     CDS             37468..37992
                     /locus_tag="PK502_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPG99431.1"
                     /translation="MANVLDAYSINFKGLKLGKHLFSFQLGRSFFSAFEEGEIHNGEL
                     VARVSLNKQSRLLEFSTHIEGMVEIACDRCLEQFNFPVEFDGKLIVKIDASQESDSDE
                     IIYLSENDFEVNLAQYLYESIYLSMPIERYHGMNGTSASDCDSDMVSLLGSHGHENQE
                     VDPRWDKLKSLTSN"
     gene            38026..38211
                     /gene="rpmF"
                     /locus_tag="PK502_01040"
     CDS             38026..38211
                     /gene="rpmF"
                     /locus_tag="PK502_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPG99432.1"
                     /translation="MAHPKSKISKQRRNKRRTHYKAELPTLSTCSNCGAAVEYHRVCP
                     ECGYYRGKLAIEKSVSA"
     gene            38244..39194
                     /locus_tag="PK502_01045"
     CDS             38244..39194
                     /locus_tag="PK502_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HPG99433.1"
                     /translation="MRIGVDAMGGDFAPEAVVRGSILACKQLKADERIVLFGDKAKIE
                     ELINSLEGGSVNCFDIEPCSEVIAMGDNPSKSFSKKTDSSIVRGFSALAAGAIDGFAS
                     AGSTGAMMVGAMLVVKQIPGVIRPAIAGFIPVSDNSVNLLLDVGLNPDCRPDVLYQYG
                     ILGSIYSKQVLGVENPRIALLNVGEEDEKGNLVTKSAFEQMKDSADFNFVGNIEGHNL
                     FDADKADVVVCDGFVGNIVLKEAEAFYHLLKKLSPNSSFTERFNSENYGGTPVLGINK
                     PVVIAHGASSPKAIMNMILQTREVISSNLCEKIKQAFNKW"
     gene            39188..40195
                     /locus_tag="PK502_01050"
     CDS             39188..40195
                     /locus_tag="PK502_01050"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPG99434.1"
                     /translation="MVKITAAITGVGGYVPDYILTNDELSRMVDTTDEWIMTRIGIKE
                     RRILKGEGLGTSEMGVMAVNELLQKTNTRPEEVDMLICGVVTPDMQFPATANIISDKC
                     GIKNSFGYDINAGCSSFLYSLVTASKFIESGTHKKVVVVGADKMSSITDYTDRATCPI
                     FGDGAGAVLLEPNYDGLGLLDAKMQVDGSGRKHLHQVAGGSCYPASFETVAKRQHYIY
                     QEGQPVFKAAVSNMADVSVEIMERNNIKPENLAWLVPHQANMRIIEATAHRMGISKDQ
                     VMINIQRYGNTTSATIPLCLWEWESQLKKGDNIIIATFGAGFTWGAAYIKWAYDGNSK
                     H"
BASE COUNT        10283 a         8824 c         9554 g        11657 t
ORIGIN      
        1 gaccgtgcaa gaatctactt gtagctaggc ttaagggttg tagtatgtgt gcagctttac
       61 cgtaaaatgg ggaagagtaa tttttttatc ggcggtaagg aggccgcgat ttataaacga
      121 cgaagctaca aacttcgccg aacgggagta ggttcgatgc agagcttgca aggttatgga
      181 acaaccggct ctaaagctcc ttcccgataa ggaatacttc atcaatgccc ttaagcgcaa
      241 gggctatagg gaataaaaaa ataaaggtgg tttaatccaa ataaaccacc ttgtgcgttc
      301 taccgttgtt gggtattacc caagcagtcc cttcttcatc ttttcgccta ttgccctaag
      361 catgtcatcg gtcatggttt cgaggttcca cttggggctc cagccccatt cctggcgggc
      421 gcatgagtcg tccatgtagt tgggccagct gtcggcaatg gccttcttaa ccgggtcaac
      481 gctatactcc atggtgaaat cgggaatccg tcttctgatt gccgagtaaa cttgtgaggg
      541 ttcaaagctc atggcggtta cgttgaagct gtttcggtgc ttcagcttcg aggggtcggc
      601 ctccataagc tgcacgcagg catcaagagc atcgggcatg tacatcatgt cgaggtaggt
      661 tccctcgggc aaagggcaaa cgtagtgctt atgctgaact gcaccgtaga aaatctccac
      721 agcgtaatcg gtggtacctc caccgggaag ggtaacatta gatattattc cgggaaaacg
      781 aacgctacgg gtatcaacgc catacttcag gaagtagtaa tcgctcagca gctcgccggt
      841 taccttgcag atgccgtaaa ttgttgtggg gcgcatcacg gtatcctgtg gggtgttgtc
      901 gaggggggat gttgggccaa atgcgccgat agagctgggg gtaaaaacgg cgcagctgtt
      961 ctccctggca atctcgaggg agttcatcag ggcacccatg ttaacatgcc aggccagctg
     1021 gggttttttt tcgccggtgg cggagagcag tgcaaccagg ttgaaaatgg tatctatccg
     1081 gtatttcttt acaatttcga gatacctgtc ggcctttagg gcatccagct cctcaaaagg
     1141 accttcctcg gccaggtgag tgcaggcgct tgagttgata tcagctgcca caacatgctc
     1201 gtttccgtaa atttttctaa gatagggaac cagctcggag ccaatttgcc cgccggcgcc
     1261 tacaaccaga atattcttca tttttttagt atagttacct tgctatttaa caatatgtta
     1321 tgaagttatg tagccattct cgttaaaaaa tccgaaagaa aagtcaacgg gtaaagcaat
     1381 gcttgaattg ctaccccgga tgaatgcctt tttctatcaa atttttacca aattggtgat
     1441 attgattaaa atgttgagca aaagtatagc ttttgcttaa tagcctgtat ggtatttagc
     1501 atattttggt aaatattttt ttagacatca ggatggttat ttttggaatt tgggtaagga
     1561 atagcatttt taacagccag attcaatgcc accttaattg atttttaatc agcattcgga
     1621 accaagtcga ggtcagggta ggtggtaggt tttttacggg tggggttgaa aagaaaaaga
     1681 ggggcaactc ccctcttttt tgcacaagct attttaggat gttatgctat ctacttcttt
     1741 ttgggttcaa ctccgggttt tgcgatgctg tcgcgcatgt tggtatcggc ttcaatattt
     1801 tttatccggt agtaatccat aatacccagg tttccgcttt taaaagcatc ggccatggct
     1861 aatggaattt gagcctcggc ttcaataacc ttagcgcggg cctcctgtgc tttagccttc
     1921 atttcctgtt ccagggcaac ggccatagcc ctacgctctt ctgctttggc ctgggcaata
     1981 tttttatcgg cgttggcctg gtccatctgg agtacagcgc caatattgcg tccaatatca
     2041 atgtcggcga tatcaataga aaggatttca aaggcagttc cggcatccag ccctttgttc
     2101 aaaaccgttt tggaaatctt atcggggttc tccagaactt ccttgtggtt ttgcgacgaa
     2161 ccaatggcgg taactattcc ctcgccaaca cgggccagaa cagtttcttc accggcacca
     2221 ccaaccagct gcttaatgtt agccctaacg gtaactcttg ccttggtaat aagctgaatg
     2281 ccgtccttgg ctacagcggt aaccggtggg gtatttatca ccttggggtt tacggacatc
     2341 tgaacggctt caaaaacatc acggccagcc aggtcaatag cggttgccat tttgaagtcg
     2401 agcgggatgt ttgctttttg ggccgaaaca agtgcatgaa ccaccttcgg aacccttcct
     2461 ccggcaaggt agtgggcttc catttcatta cggttcaggg taagcccggc tttagttccc
     2521 tcaatcatgg cctgcacaat tacccgggga ggaaccttcc gccagcgcat gaaaattagc
     2581 tgcaggagtg agattctgac accggaaaca agcgcctgaa accacagacc taccggtaca
     2641 aagtagaaaa taatccataa gccgacaatg cagacggcta taatggtaag tattgctcca
     2701 acttggctca tgtaatctat tttttaggtt taacaattat tttgctgcct tcaactttaa
     2761 ctactacaac tgttgtttgt gggtcaacta gctcatcgag ggcggtaacc tcaacgtcat
     2821 gtccatttat ggtggctttt cccattgggg caagccttcc tatagtaact cccgtgtcgc
     2881 cgggctggat gtgctgctcg taatccatgt tggctttaga atctatgctg gtgttaaggg
     2941 tgattttttt ccatgttttt gaacgtaacg acaaaactat ggtaataacg gaggcaacga
     3001 tagaaaatac aagggttata tgaccagcta tggttccatg ctctgtgtag gcggcaaata
     3061 ctccatagcc aatcagcagg aatccggcaa ttccggctac agtaattccg ggaactagca
     3121 gcacttctgc aaccagaagg aagagaccca ggagaattag taggaatatc caaatcattg
     3181 aggattgcaa ttttttagtt tagattgatg gaagcgctta gtccccgccg gtgtagctcc
     3241 tccatcatag gctcaagttc tggcaagctg ccacgtttaa tatcgcattt acccttaaaa
     3301 tgggtgatta aagcgcattg ctcggcctgg ttttcatcct gccggcatac ctctaccaac
     3361 gcctgaataa cgaattcaaa tgtattcaca tcgtcgttat gcaggaatag ggtgtaaacc
     3421 tcggaactct tgttctgggt gtgttccaaa ccaaatgttt tttcttttac cttcatgccc
     3481 ggctacagct tgtcttttcg gaaagtcata aaaacgccac tttttttaac tccatatctc
     3541 agtaagaact agaggttctg gttataaaag tgtttgtttt ttaagaatag tccaggttgc
     3601 taatatagca aatttttatt gattcaactc gatgactttt gcattaagaa ttccgccggc
     3661 aatcaggtta tccttgttac gctcggcttc gctaagggtg gagaagttcc cgatggagta
     3721 aaccatcagc cctctttcat caaccctgcg ggtaatatcc ttgttgggga ttagcgttcg
     3781 gatggttttt agctcatcgt tgggcatgct gccctcgtaa acccccagct ggatcgcaaa
     3841 aacacccttt cggtcggaat ctgacgactt ttgctgttgg agactacctt caagctgctg
     3901 ggcgcgctgg aggctaacct gttgtccgtt gtaccaagca acaataaagg catccctaaa
     3961 gcccaccgat ttcaccttcg aaagagcact ttcggcatcg gcaagccttg cgaatacacc
     4021 ggcatagtat cgggttagct tgccaacggg ttcggccgag attgggtata tacccttaaa
     4081 aaactggggt tggcgtggag tgccgtaaac tcccagttga attttatagc aaatcccatt
     4141 tggaagagcg ggtacggccg gaataggatt ttcttcgttg tatgggcttg ttttggatat
     4201 accgaaatcg gaggagatct cgctttcaac agccaggttt tcgatgttca cagggctgct
     4261 taccctttca atccttggcg gggtgtatga cgaggcagct gcctgttgtg gcttgctctg
     4321 ctctgccgat aaaagttcaa tagctgaggt agagccaaat gccgatcgtt caaacttaat
     4381 aatgctgcta gtcaacttct gctcaaagag ctggaggtgc catattttgg caaaggccag
     4441 gtccagctgg aggagcccaa gttctctaag caagtttgcc tcgaaggcgc tttcaaccct
     4501 tccgtcgtta cccatgttgt tcagaattgt gccggctgct cgaaagtgtg agtaggcagt
     4561 gctgatgcac ttgcgaatgc tttggtcgtt tgatccgctt ttcaccaggg ccacatgtac
     4621 gcagtcgttg taaatggggt attttttttc atagtatttc cccatctgct cgttgtagct
     4681 ttgcattgac gatagcatat ccgcatactt ggtatcacaa acctttgtcg aggagtcggt
     4741 tgagtttttt ttgcattggt cgtatatatc cctgttggtt aaggctagat ttcggtttcg
     4801 caatacctgc gggtatagtt gttctgcttg tgccaaacgg ttcagctcgt cggtttgaaa
     4861 gacagtaggg gcaaattgag cctggtagat gtagtcgggg ttgtccgaaa caaacgatag
     4921 gctttcgtcg cccttaggtt tcctttttcc agtaaggtat tcctgttcaa tttggctggc
     4981 acgtacaaac atgaggtcgg cctccttttg ggcagccagc atgtcatcct ctacctgaac
     5041 cacctgtttc tccaaccgaa tgcgttgttc ggctgtggta acgtaatcaa attttccgcg
     5101 tagcccctcc agcttaattt ttagagagtc agccagctgc tgggccgcaa aaccctttgc
     5161 tatggttcgg gagtattccg gatcgttttc gaccgagttg aagctggctg gtttttgtga
     5221 agcagctata ggtttttcgg cctggtcggt ggttggattt tttgttgggg cattcaatgc
     5281 taaggtcgaa actttaagag cagctattga atcgagttcg ctataggagc ggggcgagcg
     5341 gcgtataggg ttttgctcat tccgcatgag tatgacttca accgtatcgg gcattacctt
     5401 gcggttggtg gcaaaacagg ccaggctatc cgaatcatcg gccacaaaca gaaaatcgtc
     5461 aaagggtgaa ttaatcggga aagctaaatt ttcgggaggg ctccagcgtt ttgtagtggg
     5521 gtcgaatacc gttctgaaaa tatcgtatcc acccatgccg taatgcccct tggaggcgaa
     5581 gtatagtgta acgccatcgg gtatgatgta ggggtagtcc tcatcaaagt ttgagttaac
     5641 cacgtcaccc aaattttcgg gtttgctcca ggatccatta tcctgaagct gaatgcggaa
     5701 aatatcctta ccgtaggtag ttgtgctgcc gtagctggag tagtaaacgt aagtgccagg
     5761 agttgggttt gtggggtaga acattagcgg ttggtgattt tcctttatgt ctgtaggggt
     5821 gagtaagttt gttggagcgt taacaaagct gcccgattta ggggctatgg agtagaacgt
     5881 gcaggcctcg ccagctgcaa cagcttttgt gtcgtaaact tcagggttgt agatgtaacg
     5941 ggtcaggaag ctgccattct cgcaaagggt aaccagctgt tcaacctcct ccaacttaat
     6001 gtcggatgcc ccaccgctga tggtaaacct tctgtagtaa tcaattgcct cgctaaaccg
     6061 gtagctttgc ctgagtgctt cggccaggta gaagtaaact atgctgctta catcacccgt
     6121 tgaggcttgc gaaaggaatt ttaccgccaa atccatacgg cgggttccgt agaggtaaca
     6181 aattcccagg tttagttgaa tttggggatc gcgggagtgc tgctccagca accttgagta
     6241 aatgggtagc gcctgcgcat actgctggtt tttcagaagc gaatcggcca tgacacgctc
     6301 cgcatttgcc tttgttcctg tcatgcctat ttcctgacca ttggcagcaa gggctataca
     6361 ggagaaaaaa agtatggcag ttattctggt taaccgcata ggctggatta ggatttttac
     6421 gaaaatcaaa aaacattcaa agttacacga aaaagttgac aaagttgaga gctgccgggt
     6481 tgtaatggga aatgggttga aaatgacaca ccccaagctc cacgaaataa gtatctatag
     6541 ctgtacaggc gatttgtgtt tctggcttca aaatagcgct gttcgtccca ttcccaacat
     6601 tggtttttct tctatcgtta acgggataaa atgtaaagat tttcctacta ttgttgcgcg
     6661 aaattttgaa cggctttacc tttgtggaat ttcttatggt aagaaaatcg aagaagcata
     6721 aaagttggtc tgtgcccgta gcagttttcc tctctgctgc cctgatgctg ggcattattc
     6781 gatttatttc caaacctgac cataggtatg ttattcgggg cattgatgtt tcggcgcata
     6841 ccggaagcat cagctgggaa aagctaaagg atcaacgtgt ttactttgct tttgtgaagg
     6901 cctccgaggg tgccactctg aaggataaaa attttcagga taatgttaat ggagcccggg
     6961 cggccggcat tcctgtaggt gcttatcatt tttttaactt caaccgggat ggcctcaccc
     7021 aggcaaataa ctttctttct accataggta ggcaaaagta taacctgccg ctggttgtgg
     7081 atgtggagga gtggggcaac aacatccgca ggtcgcgaag ccaaatcatc gccgatctac
     7141 gcgaatttgt tagtaaagtt gaaaagaacc ggtatcaaaa aattattatt tacaccaacg
     7201 aggattgcta taagctttac attcaggatg tttttccaga taacgatatt tggatttgtt
     7261 cgtttaacca taagccaaag ctcaacaggg aatggacatt ttggcagtat gagcataacg
     7321 ggaggcttaa gggggtaaag ggcaaggtcg attttaacgt ttttaggggg agcatgctgg
     7381 agtggaggca atacatagag gatgggcggt aaacatctgc tttttttacg agccccattt
     7441 cgttattcat gggatgctaa atcagaaacc ataggtaaac atggtagtgg tagcaatcat
     7501 gtaatcaaca cggtaaaacc aaattaaccc agcttaacag caacctgctg caaaaatttg
     7561 ctatttttgt cgcagaaata aatttcagga ataatggtta aaaacaccga tttatgctct
     7621 ttttgtggaa ggagccgaaa ggaagtaagc ttgctgattg ccgggatgga tgggtttata
     7681 tgcgaccttt gcgttgagca ggccagcatc attgtaagtg aagaacttaa gaaaaagggc
     7741 gagttcaagc tgcagaagag caagctgatg agacccaccg agataaagaa attcctcgac
     7801 cagtacgtta ttgggcagga tgaggccaag aaatacctgt cggtggcggt ttacaaccac
     7861 tacaagcgtc tcctgcagaa ggatatgggc gatgatgttg aaattgaaaa atcgaatatt
     7921 ctgctggtag gcgaaacagg aacaggcaaa acacttttgg cccgtactat tgcccgtatg
     7981 ctgcatgttc ctttcacaat agttgatgct acagtgctaa ccgaggcagg ctacgtggga
     8041 gaggatgtgg aaagcattct tactcgtttg cttcaggttg ccgactacaa cgttgaggca
     8101 gccgaaaagg gcattgtgtt tattgatgaa attgataaaa ttgcccgtaa gggcgataat
     8161 ccgtcaatta cccgtgatgt atcaggagag ggagttcagc aggcactgct caagcttctt
     8221 gagggctctg ttgtcaacgt tccgccacaa ggtggcagaa agcatcccga gcagaagatg
     8281 atagccgttg atactaaaaa cattcttttc atttgcggag gagccttcga gggaattgag
     8341 aaaaaaattg cccaaaggct caataccacg gtggttggat acggtgcagg gaaaacccgt
     8401 gaggtcattg ataaaaacaa cctgctacag tacattgctc cgcaggatct acgagcctat
     8461 ggtcttatac ccgaaatcat tgggcgcctg ccggtgctca ccttccttag cccgttggac
     8521 aaggttgccc ttaggaaaat tctcaccgaa cccaaaaatg ccatcatcaa gcagtacatc
     8581 aagctttttg agtacgatgg cataaagttg cagttcgaag aggaggtgct agactacatt
     8641 gtcaacaagg ccgtagagtt taagctgggg gcaaggggac ttaggtctat ttgtgaggcc
     8701 attatgatgg atgccatgtt cgatttacca acagggagtg aaaaggagat tactgttaac
     8761 ctggattacg cacagcaaaa actcgaaaaa tcgaacctga agcggcttaa ggctgcttag
     8821 gagtgctgcc gaacataatc taaaaaggag atacccggcc cttcggtcgg gcttactttt
     8881 tcttccctta caactttcca ctcgttcggg tttaatgcgg ggaaaaacgt atcaccctca
     8941 tagttagcat gtaccaacgt taggtatatt ctttcggcaa ggggcaatgc ctgccggtaa
     9001 acctccccgc cgccaataat aaaaacctct tcatctgttg aggctatctc cagggcgtcg
     9061 gccaacgatt ttgcagctgt gcaaccctca aacttcaagc cctcgctacg tgaaattacg
     9121 atattatgcc ggtttggtag tggctggcct atactttcaa atgttttacg gcccattatc
     9181 accgtatgtc cggttgttag cttcttaaaa cgttttaaat cttcagaaat atgccaaagc
     9241 agctggttgt ttttaccaat taccctgttt tctgaagttg caacaactat ggagataacc
     9301 attcggaatt attttagaat tgtagcggta aagttagcat caattatggg tagtttgcaa
     9361 gagctgattc ctgttgagca ctttaacggt tgtaaaaaag ttaaaacatt ctggacattg
     9421 gctttggcaa tgaaatgcag atgtggttta cgttgttgtg tcaacccaaa tgattgctac
     9481 ctttgtagca aacatctata aaatgtcggg agtaaacgtg ttaatcattg gctctggagg
     9541 gagggagcat gcgctggcat ggaaactttc acaaagccca aagttaggaa agctatttgc
     9601 tctaccagga aatcccggaa ctgcccggtt gggtgaaaat atacccctta gtgtgctcga
     9661 ttttgaaggg ataaggacag tagttctcga aaaaaacatt aacctggtag ttattggtcc
     9721 tgaggagcca ttggtaaagg ggttgtataa ttttctaacg ggtgatttag ggcttagaaa
     9781 tgttttggtg gttggacccg gcagggaagg tgccatgctt gagggcagca agaactttgc
     9841 caaggcgttc atgcagcgcc atggaattcc aactgcaagg tacagagcat tcaccaggga
     9901 tgaatacagc gaagccaaaa aatttctggg aactctttcc tctccttatg ttttgaaagc
     9961 cgatgggttg gctgccggta agggagtggt tatccaagcc tcatatgaag atgcgtgtgc
    10021 gagccttggc gaatttttca gcggaaaatt tggtgatgct gggaacgtgg tggttatcga
    10081 ggagtatctt aggggggtgg agctatcggt ttttgttctt accgacggca agtcctatgt
    10141 tattctcccc gaagctaaag attacaaaag ggttggagct ggtgataccg gcccgaacac
    10201 cggcggaatg ggtgctgttt cgccagttcc ttttgccact ccggaattca tgaagaaggt
    10261 ggaggaaact atagtaaagc caaccattag cgggttagct agcgagggaa taccctactg
    10321 tggctttatt tttttcgggt tgatgaatgt ggtggggaat ccgttcgtgg tggagtacaa
    10381 cgcccgcttg ggcgatccgg aaacccaggt tgttttgcct cgtatcaaaa gcgacatgct
    10441 tgagctgctc atgctttgtg ctgccgggaa aatgaacgag gccgagctgg ttgtagctcc
    10501 ccaatttgcc gctacggtaa tccttgcctc gggaggatac ccgggtagct acgaaaaggg
    10561 gaaagctatt agtggtttag aggcttctgc caacgcaatt atttttcatg ctggtaccac
    10621 tagctccggt tccatgacgt taaccgcagg aggtagggtg ctggcctgta ccggtacagg
    10681 agaaagcatg gaagatgctc tggcctcatc atactgtgct gccaatgcaa ttaccttcga
    10741 aagcaagtac ttccgaagcg atataggtat ggatgtttac ccaaaatcat agctaacacg
    10801 ctaaccagat gctgctcaag ttcttcagaa ggtttaggat ttctactctg cttgtcatgc
    10861 tgggagtaat aacgcttttt tgggccggaa cgcttatgca gaagtacccg ccttccactt
    10921 ttactttcga tagctttgga atgccgcttt atgtgcttat tgtcaaaatt ttatcgccgt
    10981 atagctttct gtctaaactg gttgcctttg ctcttaccct taccgttgct atttacctgc
    11041 tccagtttaa cgtaaggcat acggttgttc ggcagcgtac ctaccttcct ttcctttttt
    11101 tcgtcctggt tgcctcgtcc ttaacaccgc ttcaacgttt aaaccctgct atcattgcta
    11161 atatttttat actcgtttca cttagccatg ttatctcaac ctatcagaat tctgaagcgc
    11221 tcgaccacct ttttcgctcc gggctactgc taggtatagg aactctactg tatgccccta
    11281 cggtagtatt tgttttcctg attttctgga gcattgccac cttgaggcag tttaacctgc
    11341 gtgaatggtt ggttaccctt gtgggcattt tggttccgtg gggagctgtt gttttctact
    11401 tctactggtt tgatttaggt gcatccgaac ctttcataca gctggcaaac agcctttgcc
    11461 ttgaagccga aagttctgcg gacaaaattg ccccgattat tctgtgtgta gctgttggcg
    11521 ttccgttgct ggttgcaatt ctacacatgt taaccggtat gggaagtcag aaaattgcca
    11581 tcaggaagca tcagaatatt atgcttttat ttactgttgt tgccttgctt tccttctttg
    11641 ttgtgccgtc ctgctcatac gaaatattat acattgcagc agctcccatt agcctgttgc
    11701 tttccgagta tttttccgac gcaaagggta agtactggcc ggaaatctta ttcctgattt
    11761 ttattttagg tttagtatca atgcaacttt accccctgct gatgcacagc ctctagccgg
    11821 tgaggtttga ttttttttac gactctacag ggtatttacc caaaacgact gttgctacaa
    11881 cttgcagcaa gtttggttta taagctgatt ttgaccctgg cagctgctaa aaccattttt
    11941 tcctgcgaaa gaggacaatt cccagaatgg agaaaaggaa tgatgcaaga accaccaacc
    12001 caaagcccca aggattattt tccagcccgt ttggcacatt cattccgtag atgctggcaa
    12061 tcaatgtagg aatcatgaga ataattgaaa tggaggttag ctgcctcatc acgttgttca
    12121 ggttgtttga tataaccgaa gcgtaggcat ccatcatacc gctaagaata tccgaatgca
    12181 cacttgttgt ttcctgagct tgccgtagtt caatctctac atcttctata agatcggggt
    12241 tatatgtatc cttatagttc cttaaatttt taatgcggtg tagcaaaata tcattcccct
    12301 ttaaggaggt ggtgaagtaa accaggcact tctcaatgct cagcagcgtt tgcaagtcct
    12361 cgttcttaat agatttttcg agttggtctt cggcgagcct gatgtcgagg tttatttgtt
    12421 taaggtactt cagaaaccat acgcttgatg agagcataag cctgagcacc aggtcgaagt
    12481 ggtcttttat gacaatgccc ttacggatgg tgaactgaat gaaatcgggg ataacctctg
    12541 ttcggtaaaa ggaaatgctt acaaaaatgt catccttaaa aattatacca acgggaatag
    12601 tggtaaaagg aaggcttaca tctacatttt tatagggcat tcggagcagg atgaaaaacc
    12661 actcatcctc aaattctata cgtggacgct cgtccacgtc ctcgatatcg ttaaagaaag
    12721 cttccggaac tcccagctcg tcgaaaaggt attttcgctc ctcttcggtt ggaatttcga
    12781 catccaccca gcagtagggc tcccattctt cgctgggaac aagcccaaga tctcttttcc
    12841 agaatgattt catgtactaa cctccatgtt taaatgaatg gagtctcagt acatgttcct
    12901 ccattcgggt ttaacggtca gggtgataat cgtccatata ttcaaattat tgaacggcgc
    12961 aaagatataa ttttaatggc aaagaagatt tgttaaaaac ctatattttc tttcaacgtg
    13021 cttatttcat ttatggagtg atggtagcag gttatcaggg aagctataat ggttgtttaa
    13081 ccgcaaaatc aacctgaaaa tggcaatgaa attattgcat gcactacttt ttccaaaagg
    13141 tttaaacatg gtggcttgca agctttgcac cggcatcggt ttaagcttaa ccttccatta
    13201 gcttgtttgc gatttttccc aagaagtcaa ccgcattgtc tattagctca tcggggaaat
    13261 cgtactcggg ggtgtgtata tcaggatggt tttctccggc accaaggcca aaaatcaccg
    13321 aagggaagca ggtggagaaa tgtgcaaaat cctcgctcca cctgttgggt tgctcgagga
    13381 aattcacctc gagactagct tcatgcgcaa ttgcctcaag ggtgtgggtt agcgttttat
    13441 cacacaccga ggcggggtac tcatcggtaa acgaaacgtc aatacccagc ttatgcttat
    13501 gggcaatgga agttgcggtt tcggctgcaa ttttcgacag cagggtcatg tcgtcgtcgt
    13561 caaaggctcg cagggtcgcc attatggttg catttccagg ggatgttcca aaagctatgt
    13621 cgccaacccg aatgtggata accgttacca ggatgaagtt tttaaagtgg gtggtgcttt
    13681 gaatggatag aagcgactgg atgatttccg cagtagccgc ggcagggttc aacccatgct
    13741 ccgggtaggc cgcatgcgag ggcttccctt taaggttgat gaccagcccc tttgatgccg
    13801 ctgagaaggt gtaccgccca aggatgatgc ttcccatttt gtaacccggt aggttatgaa
    13861 gggcaatagc atagtcggga tttagaccca gctcttttaa cccttccagc gactttcggg
    13921 cgccctcccc attttcctca gagggctggt agaaaagaat gacccgccct tttttaggtt
    13981 tgctttggct aaacctaacg gcaagtccag aaataatggt catgtgccca tcgtgcccac
    14041 acacgtgtga aactccctga acattcgatt tatacttgac gtcattggtc tcggcaattg
    14101 gaagggcatc catatcgcag cggaacagga cagttggccc tggctctttt ccctggtaga
    14161 ttacggccat tccctctcct gcaatgttcg agtacagctc atcggggtta tacctactca
    14221 ggaaaaaacg cagccgtttg gccgtggcgc tttcatgtcc ggaaagttca ggattcctat
    14281 gcagctcatg cctcaaacga acaagttctt ccatgctcac ttgtagtttt tttgtttaaa
    14341 ggtaagaaaa taccccataa actcaaacct ataggtatga aaaacctggt aatttttatt
    14401 gaactcttga tgggcggggg ataacgggta aaattgaaaa acctgtcagc tattcgtaga
    14461 tgacaggttt ttatgttgcc cggtgtttac caggtcatgc tatgtaaaga ttatttgtga
    14521 tccctcaccg gctgcaagct cgtaaagttg tccgacggta atgatatcct tcacatgctc
    14581 gctgaagtct tcctttttaa gaccaaacat atcgacggca agcttgcagg catatatctc
    14641 gcctccacca gcggtaatca gctccagaaa ttcctccaca gtaggaatat caagtttttc
    14701 catttggctt ttcatcatgg ccgatacacc agcttcaacg cctggtagta tgcccagaag
    14761 tgttggaaat ggtagtccgc cgggcatttt tagaccagga tttcccaccg cagaagtgtg
    14821 gagctttttc atgctctttt tagtaattgc atcaagacca aagaaggtga agaacatttt
    14881 ggtttcgatg ccctccataa cagcaccatt ggccattacc aacgcagcgt aaacatcctc
    14941 aatgttggct tttgcgcaaa ttattagcac ctttttaata gggtgatcta tagttcctgc
    15001 cataattaat tgttattagg ttaaacgcaa cttgttggtt tcgaaactcc ggcaatgcga
    15061 cttgcctttt tcaggggggc accggggaag agttcgtaaa accccttggt gtcaattatc
    15121 cccgattttc ctattgtgcg gatggtcatc acctcgccct ttaaggtttt atcacgaata
    15181 aattcaataa cctcgaagtg cttagggctt agggtaatgc cctcctccct ggcaatttca
    15241 acagcaatat cctttgtcca ctggtttgga tcggttaggt atccatcttc ggtaacggtt
    15301 accgattttc ctgcataaac tttttctgac atagcgctta tggtattagt taaacatcct
    15361 gttttttacc cagttcagcc aaaagccgaa gggtgaatga taaggtcttc ctaacatcgg
    15421 gtttatttaa ctccctgaat agcctgaaga gcgatttgtt gtcaagctta tcgtcgagtt
    15481 tcatttctga aagagtttcg gttatagtgg ccaaggcgtt gacaacctgg ggttgtgaaa
    15541 ggtttctaac tatggctcca acggctggca tttcctcccg taggcgtgta atatcttttt
    15601 cagtaaaatt tttcttaaac tcaattgcta gcaatcctag ctggttaaga taggagaagt
    15661 agccctcccg gtcgagttgc cccaattttt cggttactgc aatgctggca tcgtgaacaa
    15721 ttgggcctag gtctttaacc aggtccacta cactttccaa catgtccatc agctctccga
    15781 atgttttgat gttgcccaga accttgaaaa ataggtactt cagatcgtct gcttcaatac
    15841 taatattctg aacggcaagc tcatctacgg tagcttgaaa ggcgtttttg cccacaatgg
    15901 agaggtcgtc aatcaggtct tcaaccacct cgtttttttg atgttgacta accagcagct
    15961 cgatcacggt gtcgagcttc tggttaaggg catcaatctg ctgctcaata attctctcat
    16021 ccatgatatg caaccttaaa gttcctttcc tatcagggac atttgcgaat caaccggtaa
    16081 ttccttccct tttaagagta agtgccagta catccagcgg aacattactt ttccgtagtg
    16141 gttgatgcgg gtatttttca agaggccgaa gggtccaata cccggcaccg gataggttcc
    16201 tggcaggggc tcggtgtcgt agttaaaatc aatcagcgac cccttgccaa acccggtctc
    16261 gatgtaacag tttgaatggc cgtcgaattt ggccatcagc ggtctcccct cgattgacga
    16321 aatgaggttg tcgaaaacta cgtccgagga gaaatgggct accgaaccgg cctttgatgt
    16381 tggaaggttg ctggcatcgc ccagaacaaa tatattactc catttctcgg agcgtagggt
    16441 gtacttgtcg gttgaaatat agttgaggtc atcgcccata ctgcttcgct caatgaaatc
    16501 agcgccctta ttcacaggaa taatcgttag caggtcgaag ggcagctccc gaccatcata
    16561 ggagataatt tttttgtttt ctgcatccac cctttctagg taaaagtcgg gaatcaggtt
    16621 gatatttttt tcctgaagga ggtcgcccag catttttgtt gcctttggct tggtaaatgc
    16681 acccgaaagg ggtgtagagt aaaaaatgtt gaccttgtct cttatccctt tttttacgaa
    16741 aaactcatcg gccaggaaaa caaattcgag cggtgctact gggcatttat agggcatttc
    16801 ggctatgtta agaaccaggt tgcctccttc ccacgacttc agaaagtttt gaagggcaac
    16861 tgccccgttg aaggtgtaga aatcaaaaat gctcttaccc cactcgttac ccagcaaccc
    16921 gggagtttct tccggtctga tggaggtgcc ggttgaaact atcatgaaat cgtaatctag
    16981 cgtttgaccg ttagcgaggt aaaccctatt tttagccggg tcaacccgtt caatttcgtt
    17041 gataataaag tttactcctg cagggatgaa gctggccttt ggttttacta cgtcctgctt
    17101 gccgtatatc ccaaatggga tgaagagcaa tcctggctga tagtaatgta ccggatcctt
    17161 atccactatg gtaatttccc attgttccct atccaacgct ttacgaagct tgttggccat
    17221 tatagtgcca cctgttcctg caccaagtat tacgattctt ttcatacgta tattttttta
    17281 gttttcaaat ctcctataca ttaaaccata ccttttgggt atcgggggta gagctaattg
    17341 aacaaccatt ttttggcttc gccaaccttg caagtaacac ccggttgttc attcctgttt
    17401 gtgcacgctg tactacatga gggcttatca cctaaattgc cagctatttg tacttgttaa
    17461 tcaattctaa cgctatggcg tatcttccag cagtcaaaca atgcttctga ttggtataac
    17521 aaaccattta tgactgtttc aaccggctca tttttatagc agctgtaaat gcaaaggttt
    17581 ccttataaat ttcagctcaa atttcaagtc tttttaaata aaatgtatag ttttaaatct
    17641 tgttaaaaat caatattgat aactatcaac atatttgaat atgaaggctg aagtgtgtcc
    17701 gggtaccgaa tgcctgacgt gcggatttcg ttccgaggtg tttgcttcgc ttcccaatta
    17761 ccagctggag gagctgtcga ggagcaaagt gctaatgcat gccaaaaaag gtgaaacaat
    17821 tgcccgccag aacgacagta tagagggttt tattttttta agggatgggc tggccaagct
    17881 ctgccgcacc aacgctgaag ggagggagca aatagtggga attgctactc caaacgattt
    17941 tgttgggctg ctcagccttt tttcttctaa aacgtacaac tactccattg ttgcaattga
    18001 ggattcgagt tactgcagcg tggagtacga catggtaatg aatttagttg ggagcaacgg
    18061 ggagtttgca cggaatttac ttgccaagat ttcgagggtt tccgatttgc tgataggcag
    18121 caggctcgat atcgatcttc ttcaacttag agggcgtgta gcctacatac tttgcttttt
    18181 tgccaacgaa atttaccatt ccacacggtt taacctgccg gtttcgcgtc gggagatagc
    18241 cgagctcatt gatatgcggg tcgaaaacgt ggttagaata ctctcggagt ttagacggga
    18301 taggattatt cttattgagg gaacttatat tgagatttta gacaaagaaa agctaagctg
    18361 ggcaaaaatg cacgggtagt atattgctac attcctttta ttttgcgggt aagtgcggag
    18421 gcaaaaacaa agtcattgag ttcctgattg tcgctatcca acacgtcgaa cctggttcct
    18481 tcccaccatt ttttaccgtt gtagatgaac atcaccttat ccccgcagcc catcacggag
    18541 ttcatgtcgt gcgtatttat gatggtagta ataccatact cttcggtgat ttccttaatg
    18601 agatggtcaa ttaccactgc agttttgggg tcaagtcctg agtttggctc atcgcagaac
    18661 agaaactcgg ggtttaaaac gatggcgcgg gcaatggcta ccctcttttt catgccgccg
    18721 ctaatctcag ccgggtaaag cttgttggcc ccaacaatat ctacccgttt gaggcagaag
    18781 gccacccgct cctttttctc cgcaatggcc atgttggtga acatgttaag aggaaacatc
    18841 acattttcct caacggtttg cgagtcgaat agcgctgccc cctgaaaaag catgcctatt
    18901 ttttgtcgta tttctttttt ctttttgaac gaaagggcgt taaactgaat gtcgttgtag
    18961 taaatttctc cctcatccac atccagcagg cctactatgc acttcagcag gacggttttt
    19021 cccgaaccgc tttggccgat taccatgttg gtttttccct tttcaaacgt ggccgttata
    19081 ttgtcaatca ccattcggcc atcaaacgat tttgacagat ttttaaccgt aatcacgaca
    19141 gtagcagttg ggttagaatt aggttaaaca ggagaataaa gacgctgctg acgacaaccg
    19201 cattggtact tgcctggccc acctcgagcg acccgccctg aacgcggtaa ccataaaatg
    19261 cagaaatagt ggtgattagg aaggcaaata ccacggtttt tattagcgag taggtgatgt
    19321 aaaaggggat aaacgcatac cttataccgt aaatgtaggc ttgtgtagat attactcctg
    19381 aaaattctcc aaccatccat cctccggcta tcccgaccac catgcttagc atgcaaagaa
    19441 aagggttaaa aataagggtg gcaataattt taggaagaat caggtagctg gcggagttaa
    19501 cacccatgat ttcgagggca tcaatttgct cggttaccct catggtgcct agttcagagg
    19561 ctatgctgga acccaccttg ccggcaagta ttaggccaat gattgttgaa gagaactcca
    19621 gaaacatgct gtcgcgcgtg cccaacccaa tcatgtagcg gggaataaaa gggttttcca
    19681 tgttatatgc ggtttgtagg gttattaccg ctcccataaa aaccgatatt atggtaacaa
    19741 tcccgatgga ttgaataccc agcttatcca tctcctttat ggtctgttta aggtaaatcc
    19801 ttcccttctc cggctttgaa aaaacctttt tcagcaaaag aaaatactcc ccaattaacc
    19861 cgaatattct taccatcttt gaccttttta cttacaaaaa ttaatcggat tggcagaaat
    19921 cgtatttcta ccaacagcaa aattagtcaa tattaagatg gtagtaaaat taatcatgcc
    19981 acattcacag ccgtccattt cctccagtat cctggttagg gttatcgctc catttgctta
    20041 accatggtaa gcactggtta agtttattcc ggtgttggtt gttaagtttg ctcgaaatgc
    20101 ccttcctttc tggttagtgt agggttttcc tttcattacc ttaagccgaa agtttccatc
    20161 tcatgttaaa attccgtagg tttgtatttt taataaaaag caatgagtaa taagaattac
    20221 tactgtatag ttatggccgg aggaattgga agccgttttt ggccattaag cagaactgca
    20281 tgtccaaaac agtttattga tatactcggc gtgggtaaat cgctgctcca gcagacgttt
    20341 gaaaggatgg taaaaatttg ccctgttgaa aatattttta ttgtggcaag taattcctac
    20401 aagaatttaa tcttggagca gcttcccgag gtgaaggaga gccagctttt acttgagcct
    20461 atgaggagga acacggcacc atgtatagct tatgctacct ataaaattcg caagcgtaac
    20521 cctgaagcta cggttattgt ttcgcccagt gaccacctca ttctaaacga gcaggttttt
    20581 actaacacgg ttaacctggc gttggatttt gcatccaaaa ataacgcctt gcttaccctg
    20641 ggcgtaaagc ctaacaggcc tgaaactggc tatggctaca ttcaggtggg taaaccggtt
    20701 aaggggataa atgggctata ccgggttaaa actttcaccg agaagccaaa cctggaaatg
    20761 gccaaagtat ttttaagcag cggggagttt ttttggaatt cgggactttt tatttggaat
    20821 ctgaatgcca ttacccaagc gcttgaagag catcttccag aggtgaattc cttgttcagc
    20881 gaggtcgctt cgttgctcga ttcggctgat gagaccgcag ccattgagaa aacttatgcc
    20941 gagtgcaaga atatctccat tgactatggg gttatggaga aagccgaaaa tgttttcgtg
    21001 atttgctcgg aatttggttg gtccgatttg ggtacatggg gttcgctcta cacccatctt
    21061 cccgtcaacc aaaatggcaa tgcggtgatt gccagaaatg ttttacttta cgataccagt
    21121 aactcgctga tttctgctcc cgatggaaag ctggcagttg ttcaggggct tgacgactat
    21181 attgtggtcg ataccagcga tgttttgctt atatgccgca agcaggaaga acagcaaatt
    21241 aagaacattt tgaacgatgt ggcgatgctg aaggatggag aaatatatac ctagccaatt
    21301 tcagaaaagt atagtaaaag caaaaggcta tccgcacgac agccttttcc ttttataaca
    21361 agaacagaag tatgctagta ctcccataaa tcatgctcca tgttgaatag gtcggtttta
    21421 attttttccg attcctgcat ggtctgcaca ccgccaaagg tgtaatcgga aatcaaccgg
    21481 ttgtcaaaca cgttggattc ccttatgatg taggaatcga actggcgttt aaaaaagagg
    21541 tcatcaaacg aaatccgctg ggcatcatta aaagaattga aaaaatcagt tttagccagt
    21601 atggtgcgag cttcggggaa gtaaacccag aaagtttgct ttttggttat ttcgccggtt
    21661 tcttcctgat cctcctcgcc tgtttgtaaa attcgcatat catgcataat tgggcatatt
    21721 ccaactatac gaacctgcag ggttgatgtt tgcttgtcaa aaaaccagtc ttcttttatc
    21781 agcagctcct taatgccgct ccacttgatt tcacccttta tggtaacctc cacctcgtta
    21841 ccgttttcgt caatctgttt ttcaacccta tcctcggcgc caaggttttt gataacctgc
    21901 tcgtaggaca tccgggtggt gaactcatcg tcaacaggat cgtaggcggt aatttcgttg
    21961 tacttaattg cccttaccaa gcgttgaacg aggctttccc tgtcctgcat tggagtggtg
    22021 gggaagtaaa gggggtagtt cattttttgg cgcaggtcaa ccatccgcca aatacgcttt
    22081 gaccaaagaa catctgattc acgaacatac tggtagggta tgggttttcg ctgctggata
    22141 gtttcccggg tataaaaacc atcgtttacc aggctttcct tgcggtcctg ggctggggca
    22201 tggagcgttc ccagtatgag aaaaaatcca ccaaataaca caactatcct tttcatccct
    22261 gtaattttat tagtatttat tttcaaccat gctggtaaat gaaaaatgtt taatgcgcat
    22321 tatccattgc aaaatggaat agctgaaact accttatttt taacaccata tcgttcaggt
    22381 caataacatc accgctcggt ccaaccgctt ttatgtcggt aatggctagc tggcttccgc
    22441 tccgcaggtt gcttacaatt tttttctgct catcggtaaa ccttgaactg tttgactctt
    22501 tctcctgaag gaacccacca actgttgccg aaagcctgaa gctaacaatc ttatacttaa
    22561 ggtcgaagtc gaaatcctgg ggcatttcgg cagctatgcc caacgatgca gccagctggt
    22621 ttttctcggc atattttccg gtaatgccaa ttattctggg gagaggtggt ggaacctttt
    22681 taatgcggaa tgttttggtt cccatactct gtcttccccc ttcaagattg gcgtaaacgg
    22741 taatttcgca tgttgaggcg cctgctccgg gttgtacgat gtagcttccc ttaccttcag
    22801 gcctaatgga ccccttgctt attgaagcgg taaccttatc gggtggtact ccaggtattg
    22861 agatatccac agggttgtca acacccaggt aaagaacgtt cattttggtt ggggcaataa
    22921 ctacgttggg ctgaagaacg gagtaggtat gcttgaaagg tttcctgaca attgaaccat
    22981 cgggagcctt catttcaata agccccgacc agcttgtttg tccgagtgct ccagcccttc
    23041 gggaatacaa tcccctaccg ttttgggata ctgggatttc ctcctgcggc ccaattgggt
    23101 tgtagtccca ctccttagtt acagggtttt gagtggagtc gtaacggcaa aggtatattt
    23161 ttggtttttg ggtagtatcg gatgcggcga ggaaaacctc tgccttaaaa tcgctaccct
    23221 gaaataccac gttggagttt ggaatcaccg ttgccgaaag cacgttaaac ttaaaatccg
    23281 atgcaccaat ttgctgcagc aggtagttta ccacttcggc ctcggtgttt agtatatcaa
    23341 cctgaacctt ggtaagctgg ggaattacgg aaatcagagg tatatgctca aaacgagaac
    23401 tttcccatga gttgtgagtt ccatcttccc ttacgggggg atcgtcagtg tttagaatgc
    23461 tattgatgga ttttatcatt tcttcaccac gaacggggtc gagtagggaa atggcttttt
    23521 cacgaaactt aattatttta cgcttcagct caaaagcctt gccgttgccc tccatgccga
    23581 ttaggatctg ccctcctata tccaggttgg ctttatcctc tatcaactcg cttatgacct
    23641 ctctaccgtt aattgcaggt gtatcatctc cttcggatgt tttaaccacc tcaattttga
    23701 gatcctgaac gtagtcgtat atctccctgg ccatttcttt tatctcatcc gctttgcttt
    23761 tccaagggcc gacttttacg gggttaacca gcatggcctt gtcaaactcg gagtagagtt
    23821 tatcgttctt gagcctgtag ttttcagtgg tgcgcgataa cccgttgtca accagtacaa
    23881 aagcctccag aacatcagtt gagacattca gcgcaagcat agcggtcagc acaaggtaca
    23941 tcatgccgat cattttttgc ctgggagttt cttttccgtg tgccatagac cgttaggtga
    24001 gtcttaagat gcagttgatt tacttactgg tggaaatcga gttgatagcc gccagcatgt
    24061 tgccataaac attatttaga gacgtgatat tgctgttaag tgtttccagt gctgatttga
    24121 acttttcgga ttcatcaacg gtaacattca acttctttat cattccatcc accccctggt
    24181 acagcttttc ggcttccttt agccgttggg atgtttcctg aaggtgcatc tcatgaaccg
    24241 tgttaagggc cgccatattc ttatttagct gctccagctg ctggttgtag gtagtgctac
    24301 ccttggttat ggttgaaccg ttattcttca ttgcctcggc cagctgctgg taagttatcg
    24361 caaccccttc ggctgtttgc tggtaggcgt tggccagccc ctctgcagcc atgttgagtt
    24421 tgccggcaac ggtctcgcat gactccacaa tccttccccc tactttttga cctgcagcag
    24481 taagctcatt ctccaaattc tgaaccgaac cggcaactcc atccataaag ttcttgcccg
    24541 attcggacat ttcgccggcg gttttctgaa tgctgtccga aaggtgattc atggattcgt
    24601 tgagaacctg gctattctgt gagtagttct cgtggaatgt tttaactgat tcagaagctt
    24661 tggccatgtt atcggtaaac tccttaacat gaacggcagt tgtggtaata tctgcaatgc
    24721 tacttgatgc ctggcttagc ttattaagtc cttggcttac cttatcgaat agggctgggc
    24781 taatctcggc attctcgagc atttggttaa acttctcggt aaagatgagg gcaccgccag
    24841 ctcctgctcc agcatgttgc cccgagatct gcggggctgc ggtggcagct gcctggtgcg
    24901 ggggtgtagt ttcaactttc tcgggggcaa tagcctgagc tgccggaaca cttcctgcag
    24961 gcatggaggc aagaataccg gtaacgattt catgtatctc ctcggcacca atgccctggc
    25021 gtctggaact cttgtagccg tctatttcgt cctcctctgt cataccagtt agctcaggaa
    25081 acacaagtgt ccagtcaaac tcatcgggca gtggttcaaa tgcggagaaa aagaatataa
    25141 ttgattcggt gcacatccct gccataagca tagtacctgc gcccgggagg tgcagtattt
    25201 taaagagagc tccaaggata accaccgatg caccccaacc gtagaggtat ttcatgaagg
    25261 ttttccactt ccgggaggta acgagttctt caatgttaat tttcatgcta aaggaattta
    25321 tgattttaaa aacctacatg ttcaggttac ttagcaccaa ggtatgacct cacgcaacga
    25381 aatccaatat acgattttgc agaatcctgg tactcaaatg ctctggagcc aacctgaaga
    25441 aagaagctga tatccttcca ggagccgccc ctaattacct tacgcttcat ggcaggcaaa
    25501 tcgttaggtt tagcattgta cttatagtcg ggattcatat cgtgcatgaa ggaataggtg
    25561 ctttcgtcga aagcgtttgc tgtccattcg gcaacgttgc cggccatgtc gtatagtccg
    25621 tagtcattag gttcatagct accagctgcg atgggaatta atccgccgtc atcaatgtag
    25681 tttcctctga ggggtttaaa gtttgcaata aagcagccct ctatgttacg ggtgtagggg
    25741 ccaccccaag gatacatgct atgatcaaga tcaccgcgtg cggcatactc ccattctgct
    25801 tcgctgggga gcctgtaatc ctgaatggta attccttcag gggcaaggta cttgttaaga
    25861 agttgggttc gccatatgca gaaagctgta gcctgcttcc aggttactcc tacaacagga
    25921 tagttgtcga atgccggatg ccaaaagtag ctgtttgcat aaggttcgtt gaaagcgtaa
    25981 gaaaaatcgg aaatccagca tagcgtgtcg gggtaaacat taaccatgtc gcgcatgatg
    26041 aaactttggc ggttatccac cttaaccttc tctcccaggc tgttgaccac ctctccttta
    26101 tcgtagctta atgtttcaaa gttataccgg ttgtttttct gaccggcctg cttcatgtca
    26161 atccagtagt actcgtaaaa gagcttgcgg gtatcgattt cctttcttcc gtaaaaggca
    26221 tcctctttag agtagtacat gttattaagc acttccatct gctcctcgtc gttggggtca
    26281 attggtactt cccagttgag aacaggagga tctatggggt tgccaaattc atcctcggtg
    26341 atgacaaatt catcgttaac ctctgccagc atacgcctgg ctatggagtc gcgaacatag
    26401 tacacaaact ggcggtactg gttgttggtt atttcggttt ggtccatcca aaatgcatca
    26461 accgacacgg ttttagtggg tgaattgatg gcccacgcaa catcctggtc gttactacca
    26521 attgtaaacg atcccattgg gataaatacc atgccatagg gggttggttc agagaagcct
    26581 ctacgaccgg gtactcccgt taactcgcca tttcctcctg gagaacagct ggaaagcaga
    26641 attactgcaa tgcctatagg aaggagtttt ttcatactgc catatttatt tagcaaataa
    26701 cgcaaatttt ttaaactcat gaaaaaaaaa attataaaaa tcttatactt ttatactgct
    26761 ggggcggttt ctccttctta agctgaaaac tgtaacccag catgaactcg tgcgatccgt
    26821 cattatactt acttatccgg ctaaggctga agtcgtaggc ataacctatt cggaatccgt
    26881 tgaagagttc aaatccaatc attccggtaa ctgcctcatt tatacggtat gaaacgccgc
    26941 cccaaaactt tttattgtac cgaacaattg aatttaggga taatcttgac gatttaatgt
    27001 cggttactat cagtactgag ggttcaaatt gccaggtttt agatgggaac ataacgatgt
    27061 aacctccgga aatgtagtac tgaggggtat agcgtgcagg tatggggctt ttgttgattt
    27121 tagcccggtt aatatgggtt gccgaaacgc cgaagaacat gtagtcggtg gtgtagaaaa
    27181 ggcctagccc aaggtcaagg ttcacagaac tttctttctg ttggggaatt gcctggtcgt
    27241 ttcctgcaac cggatagttc cactttggat caatggtgtt gtttacgaaa cctccgctta
    27301 ctccaattcc aaggctaccg tttatgagtt ttggtttgaa ccgtgcggca taggctaagc
    27361 tgatgcccag gtcgctgtta aatccgtact ggtcgtttag tatgctcaac cccaaaccgc
    27421 ttttaaaacc aaatggtgcg atgggggcat taatgctaac agtagtgcta acaggagccc
    27481 catcgccaaa accagtccat tgaagcttgt taaatgcccc tattcctatc atttcctggc
    27541 tgccagcata tccggggttg atgctcattg gattatacag aatttggcta aattgtggat
    27601 cctgctgagc cattagaatg cccgcaaaag ggagaaacag ctggaataac agaaattttc
    27661 gggatgagct atgcatagct atatataaaa agcataaaaa atattcaacg ttcttttcaa
    27721 ttagatagta aagtaagcaa aaaaattaga ggcatacaaa tttatcaaca aatatgataa
    27781 aattttaacc gaaggcagaa tgttatacag atttttgacc aaaagggtta ccgtataatt
    27841 caaaaatata gaaaagtagg gtcaagttta acaatagcag ctacagctta accctaaagc
    27901 aaaagatgag ctgcactaga gctgctgtat tttctgaaat attcggagcc accggtcggg
    27961 cagctcctgt cggcttgcca gggtgtaatc gctatgggag catgggataa taattctatt
    28021 ggtgggaatg cccatttcag gggaaatagg aatctccatc caccagcgat cgagcgcttc
    28081 gtcgcagtag aaaaccatgt caactcctga tgtgccagtt cctacgataa acttcaggta
    28141 gtcaggtgta tttagggttt tgggaacgtt tttccttttt gaataaccct caatgaagtg
    28201 ccatgctagc tgggctgcaa gtaaggccga ctgttttgtg gtgtccgaat caggattgta
    28261 gttgaaaatt ccgaaaaggc tagctcgttc tccaacacct gcataatggg caagttgaca
    28321 agcttcctca gcgtagagcc cattaggaga gggaatggcg ttggcaggag agtcgcaggc
    28381 ccttatggct tccatgtcga agcttacaat gtcggcattt cgaagggttg gctcaacctc
    28441 gcgatagcta ctcctgacat agccaagccg taaaagttcg tgattgtttt tctgaaacag
    28501 gttaaccata tctccgccaa catagtagcc ctggtagcca acaatgctta attcgaaaag
    28561 cgagcgcagc ttttcgccca gcagcctgtt gacatagtta cgtgaatgaa aatcagacaa
    28621 gtcttcgtcg gcatcaataa gacaatcgat aatggaaatt gttaccagcg atttgttaga
    28681 ctttacgcct tcgtaaaggc agtaagttaa ctcctgggtt ccgcccaata ccactgttgc
    28741 tgctccggtc tggaatagaa tttctacaac tcgatttaag gcctcatagg tttttaaggg
    28801 ggtgctggtt tccaccagat cgcctgcatc atatatttta ccggctagct ttatggtcga
    28861 aagcccataa aggtactttc taatggtttc aggggagttt ttgatagatg gatttgggga
    28921 gtttcggctt tcatttacgc cgattacaac tatatcattt ttttttaggt gcttgtagtt
    28981 agttttgtta agcgggacta cttgttgctg gagcatacgg ctgttttggg agctaaagtt
    29041 aaccccctta atggctgtgg gtgttaaaaa tgacagcagc tcgtccatgg ttttaaaagt
    29101 tatatggtgg cgccaaatgg caaactattt tgtgtttcct tttttcccct gtcgcttggg
    29161 caaagttttt ttggcagtag aattcctttt acttcctttt tcagcctgtt cgtcaatgag
    29221 cttcatgcag tcatcgaagg tgagcaattt cggatctttg tccttgggta ttttgtagtt
    29281 ctgcttgttg aatgaaatgt atgggcccca tctaccattt aggatttcaa ctacttgagg
    29341 ggattcgaac tttttgataa ccctttcgcg ttctttttta cgtccttctt caatcagctc
    29401 aaccgctcta ttgagatcaa tggtgagggg gttatcgtcc tttttgagcg agtaaaattt
    29461 ggattgatgt cgaacatagg gtccaaatct tccaacgcca acaacaacct cagagttttc
    29521 gtagtttcca agggaacgag gcagcttgaa gagcgacaat gcttcctcaa gggtaatagt
    29581 ttctatcagc tgtcctttta gtaagctggc gaactgtggt tttgtttttt ttgaagcgtc
    29641 agcctctccc agctgaacca ccgggccata tcggcctata cggacgctca caggtttacc
    29701 gctaaccgga tcaatgccaa gcacacgttc acccttagtg taatccgact ccttgatagt
    29761 tttttcaaca agggagtgaa agggtttata gaattcgtcg atcattcttg tccactcgag
    29821 tttgccaagg gcaacctcat caaacttctc ctcgatgtgg gcggtaaaat cgtagctgag
    29881 gatatccctg aagtactcaa gtaggaaatc tgtaaccacc ataccgatat cacttggaaa
    29941 aagtttggct ttttcggcac cggagttttc aaccttttcc tgccgggtta ttttaccatt
    30001 tttaaggatt atggacttat agtttcgctc aactcccggt ctgtcttcct tggccacata
    30061 gccgcgggaa atgattgtgg aaatagttgg tgcgtaggtt gatggtctgc caatacccag
    30121 ctcttcgagc ttcttaacca agctggcctc ggtgtacctt gggggacgtt gggtaaaacg
    30181 ttcgttggca acaatttcca ttaccgctag ggtttggccg gcctttaccg ggggaagaat
    30241 ttcacgtccg ttttcctcgg cttcgtcatc ggacgattca aggtaaacct tgagaaatcc
    30301 gtcgaacacg attacttcgc cggaagcaac aaatttgtaa tgcgatcccg atatatcaat
    30361 gtttatagta gttttctcaa tttgggcatc gctcatctgg gaggctatgg ttcgtttcct
    30421 gattaaatcg tagagttttt gctcggtgct ggttccgctt atcttaaccc tgtcgaggta
    30481 ggttggcctt atggcttcat gcgcttcctg ggcacctttg gttttggttt tgtactgtct
    30541 gggttgatgg tatttctcac cgtattctgc tactattaca tctcttgcgg cagcaatagc
    30601 tgttgaagaa aggtttacag agtcggtacg catgtaggtt atgtggcctg cttcgtaaag
    30661 tttctgggct atcgacattg tttgcgcaac agaaaaacca tatttacgac tagcctcctg
    30721 ttgcaaggtg gaggtggtga atggtggagc gggtgccttt tttgagggtt ttttggtaac
    30781 atcctgcact gtgaaggtgg catttttgca atgctccagc agggataatg cttcctcgaa
    30841 agttgatagc ctaaccgata gttcggcgtt aaattccgag tcggccatga atatggctgt
    30901 tactctgtag tatggtgttg gtgtgaaggc aattatctcg cgttcacgtt ccacaattag
    30961 ccttacggca accgattgca cccttccggc tgaaagggcg ggcttaacct ttttccagag
    31021 aattggcgaa agttcaaagc ctaccagccg gtccagcacc cttcgggctt gttgcgaatt
    31081 cacaaggtcc aggtctatgc tgcggggtgt ttcaatggca tttagtatgg cgctttttgt
    31141 gatttcatga aacacaatcc gcttggtttt cttagggttg aggtttaaaa cctcagccag
    31201 gtgccaggca attgcctctc cctcacggtc ctcatcggaa gctagccaaa ccatctgtgc
    31261 ttcggaagtg gcttttttaa gctcatcaac aacttttttc ttgtcgtcgc ttacaatgta
    31321 ctccggagcg tagtttttgg cgatatctac acctaaattc tttttggata ggtcgcgaat
    31381 atgcccaaag ctcgatttta ccacgtactc cttgcccaaa aacttctcaa tggttttggc
    31441 ctttgcgggg gactcaacta ttaccaggtt ttgaatcata ccttaataat atatggatga
    31501 aaaaatttgc acaaagtaaa tcaattggca ggtaacggca acaaatttta aaggaattga
    31561 aatatctttt gatgttatcc gtgctgtatg ttgcagatat tcagcatgat taaatatggc
    31621 ggcaggattt ttagttgacc gctctaggtt agagggaatg atttttcatc cagaaaattc
    31681 cttttcccct tcgcatgcgc ttaagatgtt ctatagatta atttagagca tcggccacgg
    31741 gtatcttgtg gatttgagtt tctacagtag tgtttttgaa cggttggcgg aaatgcaata
    31801 gtgttcctga aggtttagtg agaatatgaa ttctacttag ctatgtattg gttcggggct
    31861 tatcctcctt gaggattaat cggagcgagg tatctttggt caggtacgac catgcccagt
    31921 tgataaaaat tatcagcttg tttctcacgc taagaatgag catgaggtga agcgacatcc
    31981 agatgaacca tgcaaaataa cccttaagat gaatgtgggg taggtcaacc acagccttat
    32041 tccggccgat tgtggccatg gtccccaaat ttttatactc atacgccttt agctttttgc
    32101 ctgtttgaat ggattttaga ttttttgcca gaagttttgc ctggttaatg gccacgttgg
    32161 caacctgtgg gtgccctttg gggtacttgg gggttaccat gtaggctatg tcgcctactg
    32221 cgaatacatt atcgcatccg ttaacccggt taaacctgtc aacgcttatc ctgtttccgg
    32281 gagcccttgc agcttccggg agcccttcga tgttgtttcc ggtaactcct gcaacccaga
    32341 taaccgtttt tgtttttatg gtttctccgt tgctcagctt taatagatat ccgtcgtagt
    32401 cggctacgaa agtttctgtc agtattgtaa cgcccatttg ctgtaggtac cttcttgact
    32461 ccacctttga ggcatcgctc atgctgccaa gcgtgtttcg ggtaccctcg attagaatta
    32521 tgttgaattt tgaaaagtca acacctgcgt aatcctttgg caggatattc ttttttattt
    32581 cggcaaatgc gccggcaagt tcaactcctg ttgggccggc accaactatt gcaaggttgt
    32641 taagcgcttc gagctcgtcc tccttagccg atacaatttt ttcgaatgtt agaatgatgt
    32701 ggtttctgat ggtaatggca tcgtaggttg tttttagagt gaaagcattt tttttaatgt
    32761 tttcatttcc gaaaaagttg ttggtgcagc ccatggccag caccaggtaa tcgtagctaa
    32821 attcgcctat actggtaatg attttgctct catttcgatc aatggagagg atttcagcta
    32881 gtctaatctg aacatttttt ttcctgctaa agatgttcct taatgggaag gaaatacttg
    32941 atggttcaag ttgcgaggtg gcaacctggt agaaaagcgg ctgaaactgg tggtggttga
    33001 ttttatctac caacaacacg tcgaatagcc gctggtcgag ctttcgggca agctgaattc
    33061 cggcaaaccc cccaccagct ataataacct tttttttgtt catcctgtgg gtgcttattt
    33121 tgtatccgca tggcagcctg ttcaccacca gcttacagct gaaacctaaa gtgtttagca
    33181 ggcctgcccc ttagttatag tttaaaaatc aacgacaaaa atgcgctaaa ttttcaactc
    33241 tatgtgtaac atgggttgaa caattttgtt gagtctgtac ataatgtcgg atgaaacggg
    33301 taggcaagag tttttgcttg ttaaacctgg ccggcgtttg gggattactg aatatgtagc
    33361 atggatgtgg caagtgggat tctggcgaaa atttagcagg gcgaaaaata agggtcggag
    33421 taaaccttgc tccgaccctt gatctttgcc agctgagcag caagttagct atactttata
    33481 gagcgcaagt tgttctaagt tagaagagct agcctgttgc taaaaattct ctttttaagg
    33541 attttgcaag catctaactg agaatgagta attagaatct gatggtggca taatttctag
    33601 attacctcca tccattgttg tcatggaaac ctgttgcaac ccaacaccca caacgtatga
    33661 ccagaagtag gcatagtcgt gcagttcgga atagtaggtc aggtagtctc tttgtcctgc
    33721 aggcctggca gtgaaccccg tttcgttggt ggggtatatg gggtaattcc agtagccgtt
    33781 tccttccatc atggtgcctg gctctaacaa ttttttgaga acaagaaccc tggattgcat
    33841 atccgaaatc cctgcaggac cggcaatgaa tgtaaacagc tcattccagt cgtcaggagt
    33901 tggaacatgc caaccatcgg ggcaaacgtt attaccgctg ctaatagcca gtccgctgta
    33961 gagcctgccg tatatgttga tggagtcggg gtaagcccag tcgttataga catggacggc
    34021 ggtgtcaatc ggcgttccat cagaatagtg cgttgtttta agattttcgg ccatccactg
    34081 ctggaatcca atgcgaactg tctgataggt gttgccgtca acatcggtca gtgtggaatc
    34141 agcttgccca acgttggtgg taatgtttcc gctaacagta atgttaccct gagtggttat
    34201 gttaccctga gataccagcg atggagtaag gaattgggcg gagtccctgt taacctcaaa
    34261 gtagttttca gtaggagtta acgtctttcc ctggttggtg agcccgccaa cggcaaatcc
    34321 acctttaacc cccttctccg ttggttcctc aattagaaat tgaacctgtg ttggctgaat
    34381 gttcatgtag taaatgggct caccgctttt tgtttctttc acctggttgg tgagaccgcc
    34441 cacggcaaat ccacccttta ctcccttggt agtttcctct tcaacataaa acctcacgcc
    34501 cgattcgtaa accgccatca ccatttctcc attggcgttg gtaacggcaa aaattggctt
    34561 tgatgagtcg tggccaaggg gtgtagtaac aaccaccgag ctgtcggcca ccatcactcg
    34621 gttagtgccg acccaacccc cgccattgct gaaaacggtt tgaccctgaa tgtaaggccc
    34681 ctgaataccg ttgttggcaa caagggcata gggaaccgag ataatctggg tagttcccat
    34741 ggaggaaaag ctggattcgc ccgatctctt tacaaggatc tccatgaata ttggatcggt
    34801 ccaaggaatg gtttcaaatc cgctgctacc gccaacagca aaagctacca ggccaaactg
    34861 gttggtctca acggtttgag tttgagtgta caccactgcc ccgtcggcag cttgcgcatg
    34921 aagcttcacc tgcacctcaa cgctttgatt gccaataacc gtaccatcgg catttcgcag
    34981 aacagcctgg taggaaaatt tctgcggcgc ttgaggtgtt acctgtccaa ggacaggaat
    35041 tacggctaac aaaaggataa agcataaaag ttttgttttc atctgcttaa atttatttgt
    35101 ctgggtttaa ttagggttag gaattagcca tgagcagaac ctgcctacac caaaaggtta
    35161 ctaatagtta tactcataac ttctgtttag tgcgttattt ggtaaaatat gtaaggcctg
    35221 atactaacaa attgtctata tatatcaaga aacaagaaag tagaaatctt ccagccacat
    35281 ctctttttag tgcaggttga tacttaaaga tttaaaaaaa tacaagttac gttacgctag
    35341 attaattgtc aaattatgtt tcaaaataaa atcttatttt ttccttttat ttatcgatac
    35401 acttaaccat tcagaaaaaa tgaactattt gacaaataac caaaattagt gggtaaaact
    35461 ttcaaaacaa ctacatgcct tactttttat attcagcgtg ggggaaaaat taaataaata
    35521 gaattaattt tgccggttaa aattttttag atgcagaaga tacgaaacat tgcgataatt
    35581 gcgcatgtgg atcatgggaa gaccacgctg gtggacaaaa tgatttttca ggggaaactt
    35641 tttaaggatt acgaaaggcc aggcgagctt atactggaca gcaacgatct ggagcgggag
    35701 cggggcatca ccattctggc aaaaaatctt tcggtaaact acaagggtta caagataaac
    35761 atcattgata cgcctgggca ctccgatttt gggggtgagg tggagcgggt gctcaacatg
    35821 gccgatgggg ttttgctgct ggttgatgct tttgagggaa ccatgcccca aacccgtttt
    35881 gtgcttcaga aagcgctggc catagggctt aagccaatag tggtgataaa caaggttgac
    35941 aaacccaact gccgtccggc agaggtacag gaacaggttt ttgagctgat gtacaacctt
    36001 gatgccaccg aggagcagct caactttcca actatttacg ggagcgccaa gcagggatgg
    36061 atgagcaacg actggcaaaa accctcgaaa gatattaccc cgctgctcga ctgcattatt
    36121 gactacgtgc ctgcgccaaa agtgaccgaa ggaacaaccc agcttctggt tacatcgctt
    36181 gactactcta gctatgtggg ccgaattgcc atagggaagc tacaccgggg cagcctgcag
    36241 gcagggcagc ctatatcgct ggttaagagg aacggtgagg tagttcgaac taaaatcaag
    36301 gacctgttgg tttttgaggg acttgaaaaa aatagtgtcg aacgggtgga atgcggcgac
    36361 atctgtgctc ttgtaggcat tgagggcttt gatattggcg ataccattgc cgattatgag
    36421 aatcctgagc cacttccaac cattgccatt gacgagccaa ccatgagcat gctcttcacc
    36481 atcaacaact ctcctttctt tggcagggat ggcaagtttg taacctcccg ccatttgaaa
    36541 gagcggctcg acagggagct ggaaaaaaat ctcgccctaa gggttgagaa aaccgactcg
    36601 gccgattcat tcatggtgtt tggacgggga gtgctacacc tatcggtgct tattgagacc
    36661 atgcgccggg aaggatacga gcttcaggtt ggtcagccga aggttatcat caaaatggtt
    36721 ggtggcgaga agtgcgaacc cattgagtac ctgactatcg acctaccgga ggatgtgtcg
    36781 ggcaaggcaa tagaacaggt tacccgcagg aagggtgccc tgttgcttat ggagcgacga
    36841 ggcgacagga ttcaccttga gtttgaaatt ccttcacgtg gcatcattgg actacgaagc
    36901 atcctgctta ccgccactgc cggtgaggct gttattgccc accgggtgaa agggtttgaa
    36961 ccctaccggg gtgatttcga gacccgtact aacggctccc tggtggcaat ggagagcgga
    37021 atttcatacg cctatgccct taacaagcta caggataggg gtaggttttt cattgatgac
    37081 catgaggagg tttatgccgg acaggtagtt ggagagcaca accgctctgg cgatttggtt
    37141 gtgaatgtta ccaagtcgaa gaagctcacc aacatgcgag ctgccggttc ggacgaaaaa
    37201 atcttcctgg ctccccccat tcggtttagc cttgaggaag cattagaata cattcaggaa
    37261 gatgagtacg ttgaggtaac cccgaaagcc atgcggctac gcaaaattat tcttgacgaa
    37321 acccagcgca agcgtatcgc cagggttgca taatttctga taacccgaac tttcccttta
    37381 ggtgaagcca ccaacctaat tttcagccat tatttatacg tttgatattt aattttataa
    37441 atttgcaacc taattttatt gggtaaggtg gcaaacgtgc tcgatgcata ttcaattaat
    37501 tttaagggac ttaagctggg aaagcatctg tttagtttcc agctgggaag gagttttttc
    37561 agcgcatttg aggaggggga aatccataac ggcgagctgg ttgccagggt aagcctcaac
    37621 aagcaatcga gattacttga gtttagcacc cacattgagg gtatggtgga gattgcctgc
    37681 gataggtgcc ttgaacagtt taattttcct gttgaatttg acggcaagct aattgtaaag
    37741 attgacgcca gccaggaatc agattccgat gagattatct acctttccga aaatgacttt
    37801 gaggttaacc tggcacaata tctttacgag agtatatacc tgagtatgcc aatagaacga
    37861 taccacggga tgaacggaac aagcgcttcc gactgcgata gcgatatggt atcattgctt
    37921 ggttctcatg ggcacgaaaa tcaggaggtt gatcctcgtt gggacaagct caaaagctta
    37981 acaagtaact aagtatcaac aacagtaact taaatatttc acaaaatggc acatcctaaa
    38041 tcaaaaattt ccaaacagag gaggaataag cgtagaaccc actacaaggc agaattgcca
    38101 actctttcta cttgttcaaa ctgtggtgca gctgtggagt accacagggt atgcccggag
    38161 tgtggatact accgtggcaa gctggcaatt gaaaaatctg tcagcgccta gtagtcatct
    38221 cgtattcaac caaaaccaca acgatgagaa ttggcgttga tgcgatgggc ggtgattttg
    38281 cccccgaagc tgttgtaagg ggttctattc tagcctgcaa acagcttaag gctgatgaaa
    38341 ggattgtttt gtttggcgac aaggcaaaaa ttgaggagct gattaattcg ctggagggtg
    38401 gtagcgtcaa ctgctttgat atagagccgt gctccgaggt tattgccatg ggcgataatc
    38461 catcaaaatc tttttctaaa aagaccgatt ccagtatcgt gagagggttt tcggctcttg
    38521 ctgcgggagc aatcgatggt tttgcaagtg ccggcagcac cggcgccatg atggtcggcg
    38581 ccatgcttgt ggttaaacaa attcccggtg ttattcgtcc cgctattgcc gggtttatcc
    38641 ccgtttcgga caacagcgta aacctgctgc ttgatgtggg actgaaccct gactgccggc
    38701 ctgatgtcct ttaccagtac ggtatccttg ggtcaattta ctccaagcag gtgctgggtg
    38761 tcgaaaatcc acgcattgca ctccttaacg taggagagga ggatgaaaag ggtaacctgg
    38821 ttactaaaag tgctttcgag caaatgaagg attcggcgga tttcaacttt gttggcaaca
    38881 ttgaggggca taacctcttc gatgccgata aggccgatgt ggttgtttgc gatgggtttg
    38941 ttggaaatat agtgctaaaa gaagccgagg ctttttacca tcttttaaaa aaactcagcc
    39001 ccaacagctc atttaccgag cgcttcaact cagagaatta cggtggcaca cctgttctgg
    39061 gaattaacaa acccgttgtt attgcccacg gagcatcaag cccaaaggcc ataatgaaca
    39121 tgatacttca gacccgtgag gttatctcct caaacctttg cgaaaaaatt aaacaagctt
    39181 ttaacaaatg gtgaaaatca cagctgcaat cacgggtgtg ggagggtatg tccccgacta
    39241 catcctgacc aacgatgagt taagccgaat ggttgatact accgacgagt ggattatgac
    39301 aagaattggc attaaggagc gaaggattct gaagggtgaa ggccttggca cctccgaaat
    39361 gggtgttatg gcagtaaacg aactccttca gaaaaccaac acccggcctg aggaggttga
    39421 tatgctgatt tgcggcgtgg ttacacccga catgcagttt cctgcaactg ccaacatcat
    39481 tagcgacaag tgtggcatca agaattcctt tggatacgat attaacgccg gatgctccag
    39541 ctttttatat tcgttggtta ctgcttctaa attcatagaa tccggaacgc acaaaaaggt
    39601 ggttgtggtt ggagccgata agatgtcgtc cattaccgac tataccgacc gggctacatg
    39661 tccgattttt ggcgacgggg ccggagctgt gctgcttgaa cccaactacg acgggttagg
    39721 gctactagat gccaagatgc aggttgacgg gtctggccgt aagcacctgc accaggtggc
    39781 tggaggttca tgctatcctg cttcatttga aaccgttgca aaacgtcagc actacattta
    39841 tcaggagggt caaccggttt ttaaagctgc agtttccaac atggcagatg tgtcggttga
    39901 gattatggaa cgaaacaaca ttaagccgga gaacctggca tggctggttc cacaccaggc
    39961 caacatgcgt ataatagagg ctacagctca tagaatgggc atcagtaagg atcaggtgat
    40021 gatcaacatt cagcgctacg ggaatactac cagtgcaacc attcccctct gcctctggga
    40081 gtgggaatcg cagctgaaaa agggcgataa catcataatt gccacgtttg gcgctggttt
    40141 tacatggggt gctgcttaca ttaaatgggc atacgacggt aactccaagc actagtagct
    40201 agtaacatcc ttagtttttt tattcgaagt ttggttgaag cagaaaccct tgtcgctaag
    40261 aagacgtggg aggttgtcta tttaatctac gctgccaggc aggtaagcaa tttccttg
//
LOCUS       DAOPHX010000006        38718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_966_length_38718_cov_6.175698, whole genome shotgun sequence.
ACCESSION   DAOPHX010000006 DAOPHX010000000
VERSION     DAOPHX010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 38718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38718
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_966_length_38718_cov_6.175698"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            393..947
                     /locus_tag="PK502_01055"
     CDS             393..947
                     /locus_tag="PK502_01055"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HPG99435.1"
                     /translation="MEEKKSNLNVTMTWGLVVGLALIILSLVLYIMNIYKAPTWVGIL
                     NYALIIGGIVMAQLKYRNNILDGYITYGKAVGTGVLVSLFASIIYAFYFIILTKFIDT
                     GYMDKVLETVEQAYAENGLSEDQIAAALEMSKKMMTPIMMALSTVFGFVFMGTLFSLI
                     TSIFIKKEAPLFGSESKEGETLSE"
     gene            958..1914
                     /locus_tag="PK502_01060"
     CDS             958..1914
                     /locus_tag="PK502_01060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPG99436.1"
                     /translation="MDISIVVPLYNEEESLSELAAWINRVMTLHNLSHEVIMVDDGSS
                     DRSWAVIQELRKQYPAIRGIRFRRNYGKSAALQVGFTAASGDVVITMDADLQDSPDEI
                     PELFRMIKEEQLDLVSGWKKKRYDPLLTKRIPSKLYNFTVRMVSGIKLHDMNCGLKAY
                     RNNVVKSVEVYGEMHRYIPVLAKQAGFRKIGEKIVQHHERKYGSSKFGWSRFINGFLD
                     LLSVTFVSRFGKKPMHFFGTLGTLMFFAGGVIAVVLVANKIWSQTHMLRYRPVTDQPL
                     FYIALAIAIIGVQLFLAGFIGELVSRSASDRNTYIIEEDTGA"
     gene            2026..2772
                     /locus_tag="PK502_01065"
     CDS             2026..2772
                     /locus_tag="PK502_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPG99437.1"
                     /translation="MKALHSHRSIRRYKSTPIDESLLNEILQAGIRASTTGNMQVYSI
                     IVTKSQEVKQQLLPCHFNQQMVVEAPVVLTFCADFNRFNKWCGQRKAEPGYDNFISFF
                     TGAIDALLVAQNVCIAAEENGLGICYLGTTTYTADRIVEVLKLPKGVVPITTVVMGYP
                     DESPELTDRLPLAGVVHHEVYQDYSEGDIDQIYMNKESLQLTKKLLEENGKETLAQIF
                     TDIRYTRADNLLFSKVFLKTIEQQGFMNNK"
     gene            2831..3220
                     /locus_tag="PK502_01070"
     CDS             2831..3220
                     /locus_tag="PK502_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99438.1"
                     /translation="MKTFKTILLVLVLLLIGAGAFLYFGVYEKGVMAGRILRVSQKGV
                     IFKTYEAKLSIESFGALRSTSPIAETFDFSVERKQKELIKQLEEVALTGERVNVHYLK
                     RYMRVPWRGNTKYFAESIERLGKTQQP"
     gene            complement(3349..3696)
                     /locus_tag="PK502_01075"
     CDS             complement(3349..3696)
                     /locus_tag="PK502_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPG99439.1"
                     /translation="MNIYVSGLSYRINDDDLRQLFEEYGEITSAKVITDRETGRSRGF
                     GFVEMANNEEAQRAIDELTGAEYDGKVISVSPAKPKTDKPRNGGGFNRERRGGFDRGS
                     RGGSGNRGERRDY"
     gene            complement(4000..5985)
                     /locus_tag="PK502_01080"
     CDS             complement(4000..5985)
                     /locus_tag="PK502_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HPG99440.1"
                     /translation="MFKNHPKGLLVLALTNMGERFGYYTMLAILTLYMQAKFGLTAAS
                     TSMIYGAFLASVYFLPIFGGVLADKVLGYGKTILLGILVMFIGYLLLAIPLEGNSVGL
                     TMMIISLVLIALGTGFFKGNLQALVGKLYDDKRYDTNRDIAFSIFYMFINIGAFFAPS
                     AANYINNAVMKSSDFTYDASIPKNYVVLNDEKQAVDKNTFIVQNLTPAEQQLSGKEFD
                     AVLTKAKKKHKVVFTEDIEHALFRLKAAGLNQYTQSGKITDPAMMISNNEYELLQSRS
                     QEDMLKKAEVTAKIQAISITDPALEGGPNPDDFARNFGKHYVSESLSTSYNLAFGVAC
                     ISLIISVLIFLLFKKSWKSVDKTVKQEIAESKANGKEVKVMSKEEVRERTVALLLVFF
                     VVIFFWMSFHQNGLTLTFFARDYTQSVISPGWTMLFNLFPLLSLIVFLYGIYLSTIGL
                     LGGKPNKKSGLLLTVLGIAGLAGYYFTRVKGIEVMTKITPQIFQQFNPMFIVILTPLT
                     VALFSYLGKKKKEPSAPRKIGIGMFLAAIGFTIMLICSFGLPAPSEISGVSDQLVSPA
                     WLISTYFVLTVAELFLSPMGLSFVSKVAPPQYSGLMQGGWLAATAIGNLLVGVIGTFW
                     EKLPLVGFWVILIICCVISGIFIFSVMKRLEKATGNV"
     gene            6868..7365
                     /locus_tag="PK502_01085"
     CDS             6868..7365
                     /locus_tag="PK502_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPG99441.1"
                     /translation="MKKIVLFALAMEVLSGMAQAQDPWTLEQCIQYANENNLQIKMGE
                     LSVNMSKDKLLQSKLSIYPSANLNANHAYSFGRVTNYITNQKESRNTQSTSFSFSSSM
                     PLFEGFQLTNTKKQSNYDLLASIQDVETVKNNIALSVASAYLQILYQQELVEVAQRQV
                     EQSEM"
     gene            7603..8208
                     /locus_tag="PK502_01090"
     CDS             7603..8208
                     /locus_tag="PK502_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPG99442.1"
                     /translation="MFTVAQQTMPQILGSQYKVQSSEMGLKIAKGANYPRLTLSGSYS
                     SGAQSYLNNDNPLLTSDPFFTQIKDNASTSVGLSLGIPIFNGGSTRYRVSTARISLDN
                     ARLTLENEKNILYKDIQQAYADAIAAQKKHKASQKSKMASEESFRYTENKFNLGLLPA
                     IDYTTARNNLAKAETDLLQAKYELVFKTKILDFYKGVPLSL"
     gene            8332..9654
                     /locus_tag="PK502_01095"
     CDS             8332..9654
                     /locus_tag="PK502_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPG99443.1"
                     /translation="MKKGLLKYLIGAMVIIIVLAIVGKKKGWFGSTDAIEVYVEKPQK
                     RTITESITANGKIQPEVEIKISSEVSGEIIELPVKEGNWVEKGTLLVRIKPDTYISMR
                     DRAIASVNSANARLAQAKAQLSQSELAFNRSKQLFEQKAIAESEFETAKTNYEVAKSE
                     LKASEFNVESAQASLKEAEENLRKTSIYSPISGTVSKLNVELGERVLGTIQMAGTELM
                     RIANLNRMEARVDVNENDIVKVNIGDTALVEVDAYLDRKFKGIVTQIANTANVQGVST
                     DQVTNFEVRIFLLDDSYKDLVTDSRLSPFRPGMSTTVEILTETRSNVLTVPILAVTVR
                     SDSTAKKDITGMEKVDDSSTPIEQTNSKLNKPKEIVFAVSGDTARIITVKTGIQNNQY
                     IEIVEGLKGDEDVVTAPYSAISRKLKDKSLVKKVKTKNDLLKAEVAKK"
     gene            9912..10619
                     /gene="tsaB"
                     /locus_tag="PK502_01100"
     CDS             9912..10619
                     /gene="tsaB"
                     /locus_tag="PK502_01100"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPG99444.1"
                     /translation="MATILCMETGSTTCSVALGSESGVIGFREVSDANIHASALPVLI
                     EELIRETGFELGNIDAIAVSMGPGSYTGLRIGVSLAKGMCYALHKPLIAVGSLEAMVT
                     GVGEFINMESINNNSLLSPMIDARRMEVYTQLFNRKGEMLSEVKALVVETDTFSDLLK
                     KNTVVFFGSGASKCQQVIGNSGAIFVEHFKPSARYLLPLAQKKFRDELFEDIAYFEPY
                     YLKDFVPTRPKNRIIPD"
     gene            10579..11427
                     /locus_tag="PK502_01105"
     CDS             10579..11427
                     /locus_tag="PK502_01105"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPG99445.1"
                     /translation="MCPQGQKTGSFRINKNFELLRSRQGFVVLLACILILGIPVASAQ
                     KAFGPGEKLNYKIYYGLIHGGEAVLEVRKGDYKGKPANHIYMAGKTVGLANKFYYVED
                     IYESYTDPETDWPYFSIRNIHENRYRHYSTQVWDHWSRKDSSIVNSSKTGEVVVVKGC
                     QDILSSFYYLRNILIKSDPVKDQLFIVDTYFTDEKFPLMVRFKGRETIKTSLGEFRCL
                     KFMPVVEVGRVFKTQDDMTIWLSDDDNLVPVRVRFEIFVGAFYCDLYSFSGLKNPFSS
                     YSPKVK"
     gene            11592..12158
                     /gene="efp"
                     /locus_tag="PK502_01110"
     CDS             11592..12158
                     /gene="efp"
                     /locus_tag="PK502_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPG99446.1"
                     /translation="MATTADFKNGLCIEFNGKLYTIVQFQHVKPGKGGAFVRTKLKNL
                     ETGKVIENTFTSGVKINVVRVERRPYQFLYKDDLGFHFMHQETFEQVSLSGDLIENSD
                     LLKEGQYVEMMVEADKENILTCELPPFVEMEVTYTEPGLKGDTASSTALKPATVETGS
                     VINVPLFVNTGDKIRIDTRTREYYERVK"
     gene            12266..13513
                     /locus_tag="PK502_01115"
     CDS             12266..13513
                     /locus_tag="PK502_01115"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPG99447.1"
                     /translation="MSIATSQNRLKLSTFKLNALLGMAQAISAELKTEELVDRFRSIL
                     NDDLGIDRILLYKKEEDRWELLLNSNCPQKVVEGISVERDLMAFNEITFVGVAENPLL
                     LELDVIIPIVQNNAPVGYVLIGDTSEYEKGVSATIRHLNFVQTLSIIIFVAIENLRLF
                     HKSLEQEAMRKELELASKMQSMLIPTPESMPHYPHISIASFYNPHFEVGGDYYDVIPL
                     NDNEFGFCIADVSGKGISAALLMSNFQANLRALFSLDIPLTRLVEKLNERVMATANGE
                     KFITLFVGRYNVEKHLLEYVNAGHNPPFMIDKQSKIISTLSNGCVGLGMLDCIPVINQ
                     GAVTIDQPSLLVCYTDGLVEIMRNNKVEYNTQVIEQEIVKLEPISTTIQRIAESRGIK
                     NDGSSNPEIFDDISILGFGFNCC"
     gene            complement(13500..14939)
                     /locus_tag="PK502_01120"
     CDS             complement(13500..14939)
                     /locus_tag="PK502_01120"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPG99448.1"
                     /translation="MVTDEHKTKLAALFLGLAFVAINSLLVTKNILSFSLLPVILLLV
                     LGVVLVPVKIFYLVIALAPLSVPLSEFLPGRSFDLWLPTEPLIFALLLLTAIHLIQER
                     RFDTGLFGNPLFWAMLFYLGWLVITCISSELPLVSVKYTVLRIGYIAFFFYIPFVLFK
                     ATPQNFTKYIAFYITGLTVVVAITLYRQARIGIFDKFAAVGTCNPFYTDHTSYGAALA
                     FLIPVVVAWAASTKTLWQKILMGILILVFTVALVLSYSRAAWLSLLVALLIWVVWLMK
                     LRFRTIAILSVSIIAIGFGLRGQLAQWVGKNQTVSTGSLSDQLLSVGNITTDVSNVER
                     LNRWTSALRMFRERPFLGWGPGTYQFTYAPFQPSQFFTVESSNLGAKGNAHSEYLGLL
                     AEAGWPATMGYIAILLVAFIMGFRLLGKLPLDFPYRKLLLGVLLGLSTYAFHGFLNNF
                     LDMDKIAALFWGYLAMIAAMNTAYRTSSS"
     gene            complement(14950..15816)
                     /locus_tag="PK502_01125"
     CDS             complement(14950..15816)
                     /locus_tag="PK502_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99449.1"
                     /translation="MEQDYRTAEPSFVATVKVWKRYRLVFLIVGVAALVGSTVASLLI
                     APKFRATAVFAPTRELQVPGTPLSDSLFVEFNNFGGAVEREQFLEVMASGAFRDTLIS
                     RMGLHSYWGLQTTGDKSMQAARNLYGKHVRVKPTQFIGIAVEVTDADPQMAAAVANAA
                     VGIADSMMRSLKSEVAAKTLQALEHQYRLALMELASLQDSFANLKKSFGLAPAVELSG
                     LRQAIAFARVQVTGDIPTCYVIDRAVKPSVKVSPNRGLIVVISTLSAIFFTIFVVAVL
                     ELARGSKLNQGD"
     gene            15947..16186
                     /locus_tag="PK502_01130"
     CDS             15947..16186
                     /locus_tag="PK502_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99450.1"
                     /translation="MKNLNFTRGQVTNILEGIAKKEEGVQHILKMSIEAIKMVWRDVY
                     NKRHQDISNSMKMQILPLFLHLALGNTTYGREGAI"
     gene            complement(16364..17656)
                     /gene="tyrS"
                     /locus_tag="PK502_01135"
     CDS             complement(16364..17656)
                     /gene="tyrS"
                     /locus_tag="PK502_01135"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPG99451.1"
                     /translation="MNFIDELQWRGLVHDVMPGTADQLNKEMTSGYIGFDPTAESLHV
                     GSFAQIMLLKRFQLCGHKPIVLVGGATGMIGDPSGKSQERNLLDSATLQKNLEGIRNQ
                     LSRFLDFDSGNPNAAVMVNNYDWMKNFSFLDFIRDVGKHITVNYMMAKDSVKKRFGEE
                     TKEGMSFTEFSYQLVQAYDYLWLYKNLGCRLQMGGSDQWGNITTGVELIRRMTGGEAF
                     ALTIPLITKADGSKFGKTESGNVWLDSKLTSPYKFYQFWLNTSDDDAEKYIKVFTLLD
                     QSTVDALVEEHRREPHLRKLQKMLAREITIMVHSEEDYRKAVEASELLFGNATTETLM
                     SMDEDTFLSVFEGVPLFEVSRQHIAEGVPLSTLAVELAPIFSSKGELRRLVTGGGLSL
                     NKVRVENTELPVSNSHLLNGKYILIQKGKKSYSIIRTV"
     gene            complement(17771..18301)
                     /locus_tag="PK502_01140"
     CDS             complement(17771..18301)
                     /locus_tag="PK502_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPG99452.1"
                     /translation="MAVFDIVVVILLVVGAIRGLRQGFLSQLASVVALLLGIWGAVRF
                     SDYMATLLSEKFDIGSAHLPIISFAVTFILIVIAIHFIEKIVERFLNLAMLGGINKVL
                     GFVFGFAKYAIITSVLLVFVEKANIRFNFFTEEQKSKSLVYKPVASLAPAIFPYLHFD
                     TIKESVKKSLGNQKGK"
     gene            complement(18319..18627)
                     /locus_tag="PK502_01145"
     CDS             complement(18319..18627)
                     /locus_tag="PK502_01145"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPG99453.1"
                     /translation="MVKINELKVEQLEDAANMLKAMAHPMRIAIISLLEANGRLTVTQ
                     IHEKLQIEQSTASHHLGILKDKGLLACKREGKNNLYFLRNENLGKILDCVGSCTSTRF
                     "
     gene            19161..20129
                     /locus_tag="PK502_01150"
     CDS             19161..20129
                     /locus_tag="PK502_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPG99454.1"
                     /translation="MLEAIKQPVDEELKKFEPFFRKNMGSNIRFLDLITNYIYRNKGK
                     QLRPIIVFLSAGLNGKICQSTYVAAGMIELLHTATLIHDDVVDESFERRGAFSINALW
                     RSKVAVLVGDFLLSKGLLVAVQNKEYELLEVMSNAVREMSEGELLQIEHSRKMDINEE
                     AYYDIIRKKTAYLIASCCENGAISAGADSDSIRRMHEFGINLGMAFQIRDDLFDYQPK
                     GLVGKPNGNDIKEKKLTLPLIYALREAPAAEGRKVLREIRSRSKSSVTISTVVKFTNE
                     YGGISYATGQMLNFKQQAQEVLNSFPDSDYRQSLMLLTDYVTDRNK"
     gene            complement(20133..21455)
                     /gene="dgt"
                     /locus_tag="PK502_01155"
     CDS             complement(20133..21455)
                     /gene="dgt"
                     /locus_tag="PK502_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HPG99455.1"
                     /translation="MEWTKLLDASRFGATGATKNTYDTARTNYQHDFDRLIFSSPFRR
                     LQNKTQVFPLPGSIFVHNRLTHSLEVSSVGRSLGQNVYLQISETLSPHEKPLVAEIPG
                     IIAAACLAHDMGNPPFGHSGEEAIRKFFEDNSGVFKPMVDSWEWSDLVRFEGNANALR
                     LLTQQLTGRRTGGFRLSNAVLASVVKYPYPSASGKKKFGFFKSEANPFLSIAHATGMH
                     SVGNMEFCRHPLVYLVEAADDICYQIMDIEDAHKLGILSTPMVVDLYRAFFTDEPKSA
                     RDRITSTLKEVQDPNEQVAYLRAIVISKLVEECARIFVDNLKPIMDGEDFSSLLEKIS
                     GASHTAMEAIKNLAVERVYNHRKVVEIEIAGYQILGSLLEIFCDAVLHPQTGLSKKVI
                     RLVPNQYMTSSGDDYTKIMSVLDFVSGMTDVYALETYRIIKGINLPTL"
     gene            complement(21692..24511)
                     /locus_tag="PK502_01160"
     CDS             complement(21692..24511)
                     /locus_tag="PK502_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPG99456.1"
                     /translation="MMKSKPNARKLNLQFFSSLSLKSKILILFTGSTILILIAALIFI
                     SNQLKRQSVENAIAQIGITADQQALMIQNALEKNLYIARTLSGTAKSCSQLPPDRLNS
                     YLTSAYRDVLQSEPNILSVWDSWELKFLDPAFSNSYGRIRNMAWRSNGTILTKSETIL
                     TPDSPQYARIKKELTESVEEPYFFSFDNGSPVLMTSLVVPNIIDGKFAGVSGVDIPLT
                     GFNELIRGIDRYEGSYAFVLSTTLKFIAHPDKNLAGDDAMASYEELFTLHGVPEIIAN
                     GNSAFFEGADASGANSFVAIRPIIIGSCPQSWALVLLVPKGVVLHDATVIFWKTLLVG
                     LAGILLLTLLIQAVARKYILEPIIHVSENLSRLAKGHVDGSMKVHVEVSDELGAMSEY
                     LNRTVDGLTKKTEFAQTIGQGNYGAKMELMSDEDALGQSLIDLSANLKKAQEEVQKRA
                     ADDQKRQWTNEGLAKFSEILRQNNDRLDLLSINILRNLIDYLGANQGGLFVYNDDDPS
                     NPFFDLLAAYAYNRQKFIKKRILPGEGLIGTCAIEKQTIYLTEIPENYITISSGLGEA
                     KPRNLLIVPMKVDENVLGVIEIASFSAIMDYQVEFVEKLAQSIAQTLTTVKTNLKTAE
                     LLEKTQQQTEEMKAQEEEVRQNLEELATIKEELEKRNEDILENQKQLEWEKSLLDSLL
                     NYVPDKIYFKDLKSRFIKVSRSTLKAHGFENPDDLIGKTDFDLFGEEHARPAYEDEQN
                     IIRTDSPIIGLVEKEVFTDGRVSWAETSKLPLKNPRGETIGTFGITRDITASKLMEEE
                     VKKKEMDALAYQRDMQKKALEAQNLYEAINNSTYVIEYNPDGYITFINDAYLELLNSK
                     REDVVGKHHSYQLDFTDEQRKNYKQFWDDLNSGIARKETHRFTVGKRTYLFFETYTPL
                     KDENGKVFKIMKIAVNVSHLLHEGE"
     gene            complement(24532..25368)
                     /locus_tag="PK502_01165"
     CDS             complement(24532..25368)
                     /locus_tag="PK502_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPG99457.1"
                     /translation="MDSIRKVVLVPWDFTQSSEYALQHAIELAKAGDNEIMLLHIIPH
                     SPLFDIFYKPNFKKIEAINKQFEKVAQDVQSRFGIIPQTLVMEGSPRKIIRGLILTAN
                     VNLIVAEHGYTIKDKTYRTSDFIKNLTFKDITLPFIIVSKPPRHDHYIEIVIPVDYDR
                     KFKEAVHWIIYLAKYYKCNINLIKPFLTDDGQKKQLANNIYFTKKMLDGVGIIYGIKT
                     AKKKSFFKDEVFRFANTIEADLILVMADKYNYFVDAKREANLDIPVMCVNPRIRKYQS
                     FY"
     gene            complement(25501..26844)
                     /locus_tag="PK502_01170"
     CDS             complement(25501..26844)
                     /locus_tag="PK502_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPG99458.1"
                     /translation="METLLFQRTNELLKEREKSENLLARVLPQETADELKQFGKIKAQ
                     KFQMVTVLFSDIQGFTKITDDTDSEELIDQLDRFVYEFDSVVQKYHIEKIKTVGDAYM
                     CAGGLPKKNQTNPIEVVVVALEMMSWMKETNRRHQKGDVWELRMGIDTGPVIAGVVGQ
                     SKISYDIWGATVNTASRMESAGEAGKINISENTYLLVRDFFICTYRGQIPVKNRGNIQ
                     MYFVEGILPNLSQNLEGTLPNQDFFIELQLIRLSDLEELVLEKLEQGLPKNLYYHNVK
                     HTVDVYTQVELIGRAEGVSKEDLLLLRTAALFHDAGHLVDYATHEQMGVKLAREILPN
                     YRYSSDQIDEICKLIMSTKLPPNPKNLLEEIICDADLDYLGRTDFIPVSNMLYKEMHE
                     NGMIGSLHEWNILQMNFIQNHQYFTQTARRLRNVNKDMQLQNIKSWINQMEKSNL"
     gene            27291..28088
                     /locus_tag="PK502_01175"
     CDS             27291..28088
                     /locus_tag="PK502_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPG99459.1"
                     /translation="MKGESKTILVPTDFSQVAEYALQHAVRVSGVVKEPITILHIVKA
                     EADKAEAEARLEKIVRGTKEKYGVDITPVVRVGNIFTDIALVADEVQASFVVMGSSTI
                     KEMDENKVSWVLKVITSCKVPFITIQEPPVNKRYDEVVFPVDFTLENQEKHKWISYFS
                     DFYLSRFHLVKPNTTDPELLAKIDINMASARRFLDQKGAKSVEYTVPGLKPYEQEILS
                     LAANIRADLIVLMTTPLDSEGRFTVDPMEQYILANAGHIPVMCINPR"
     gene            28100..28681
                     /locus_tag="PK502_01180"
     CDS             28100..28681
                     /locus_tag="PK502_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HPG99460.1"
                     /translation="MPQKLVFATNNVYKLKEVQAILGNAFQLLSLHDIGFIAEIPEDY
                     ETLEENAMQKARYVFSRTGYSCFADDTGLEVEALNGEPGVYSARYAGEDKKSTENIKK
                     LLMKLNGVENRNARFRTVIALTLSGKDYLFEGTVNGKIIDSPNGFDGFGYDPVFIPDG
                     YNQTFAQMPLALKNSISHRGMAISKLVDFLKKQ"
     gene            28731..31022
                     /locus_tag="PK502_01185"
     CDS             28731..31022
                     /locus_tag="PK502_01185"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPG99461.1"
                     /translation="MARVTSAVFLFLFLLQGVLQAQIPVGSWRDHFSFGNTKLLCYEE
                     GKIYCGADNGIFSYNIATGEVDKVTRVNGLSEVGISALSYFPSLQILAIGYESGNIDL
                     LVQNSVVNLPFIKEKPLSGSKTINRFTLFNNLIYTSTDFGLVVVDPAKREIKETYYIG
                     NNGTGIRVSSMEVFGDRFWAATSNGLKSALCSDPLLVSFEHWTTETSFTNPTAECAGV
                     AAKDSYLFALERQENANDILWRFDGSSWVEVDRPLPATLHLSVGNNRILVTSANGIQT
                     YQFPGIRDEFLSPASLFINPQAAISVDADKLAIADKFNGLMFGSSQALRSIYPNGPAN
                     NNHLAIDVSSNRVVVASGGYDATFTNLWHPFMVHDFTHEEWSSFADYSYHDAVAVRVN
                     PGNPSDYYVASWGGGLYQFHDGSMVAHFTPENSSLQSIFPNAPYCRISGLDFDPKGNL
                     WVANTMVANPISVRKTDGTWISFPYASAINSDKLSTLSYSNYGTLWLLLPRGQGLFAL
                     NPGNNIDIKDDDRYRKLQPASSSGSLFNVDINALAFDRDDYLWLGTSQGVLVCFNPGK
                     VFDGGMYFQKIKIPDVVEGLAVYLLETEEVTSVTVDGGNRKWFGTARSGVFLFSPDGT
                     KELAHYTIQNSPLPSNRITDVKVHPASGEVFIATDKGLVSFRSNANQPGSGFGKVYAF
                     PNPVRPEYEGDITITGLMENTLVKITDISGNLVYETFSVGGMATWNGKNSKGQRASSG
                     VYLVFCSASQGEKTAVTKILFIR"
     gene            31026..31754
                     /gene="recO"
                     /locus_tag="PK502_01190"
     CDS             31026..31754
                     /gene="recO"
                     /locus_tag="PK502_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPG99462.1"
                     /translation="MLQKSRGIVLHAVKFRDNSLIATCYSKELGRTTIQVNHAYGSGK
                     TAGLAVYFQPLDILDLVFYKKETSEISLLKEVAIAIPLSELHFDPVKISISMFVSEVI
                     YRTVREVEPNSELYEFLENSIRLLDVMHDGVANFHLIFLIQLTRYLGFYPANGWSETM
                     PFFDYKNGFFCPSPPSHSFNLDKTSSRRLGMLLGTPFYRSNEISLNHAQRIQAINDLL
                     CYYNFHLGSPLDFKTLPILIQLFQ"
     gene            31854..32990
                     /locus_tag="PK502_01195"
     CDS             31854..32990
                     /locus_tag="PK502_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPG99463.1"
                     /translation="MKQINAEFGVDTANSLLKLINSHNAIDLTAGWTRFPIPVQVADL
                     VARELRQTHGRAPIEGTLELRKHVSEYIQKKYFRTYHPAAEIAITSGAAQAIFSAVAA
                     LVSDGDEVIIFEPTRNDTALAIEVCGARPVYLKLHEDNASIDWHEVQKSVTPRTKLII
                     ISSPNSLTGSILSPDDMENLQKLINGTKLKIISDETLSDIVYPGVIPSSVAFFPKLAD
                     NAYIIGSLSQTLGISDWDLGYCLAPAAMMNNFQRIQRAIANTANHALQLAIADFMSTN
                     ETSFPYHAVIQKNRQILLDSLSSTRFKPLPMQAGYTQTLDYSDVSDLKDVEFAEMLVR
                     DYGVGVSPVSIYFHDKTIRKHLLINLAVEVDVMTEAALRLSKTA"
     gene            complement(33046..34080)
                     /gene="rlmN"
                     /locus_tag="PK502_01200"
     CDS             complement(33046..34080)
                     /gene="rlmN"
                     /locus_tag="PK502_01200"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPG99464.1"
                     /translation="MPELLPLYGKTLAELQDIVSQEGLPPYAAMQLADWLYKKNVESI
                     EAMTNISKSAREQLVGRYYVGLSKPEGVSASADGTKKYLFRSGNGKFVEAAYIPETGR
                     ATLCVSSQAGCKMGCLFCMTGRQGFQGNLSSGEIINQLRSIPEFKNVTNIVYMGMGEP
                     FDNLENVLRSTEVLTSEWGMGWSPKRITVSSIGIIPAMNTFLSRSSCHLAISLHSPFD
                     EERRMLMPIQNVYPIADVVEEIRKANIAGQRRVSFEYIMFDGLNDSPRHVREISRLLH
                     NLRCRVNLIRFHPIPESALRSASDETIIKFRDALTSKGIFTTIRASRGLDIQAACGLL
                     STKGLLKNKD"
     gene            complement(34169..34834)
                     /locus_tag="PK502_01205"
     CDS             complement(34169..34834)
                     /locus_tag="PK502_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99465.1"
                     /translation="MKAWKEGDYRLQQVISNTRLAPGAFLLKFRKQFDFSAGQIVALG
                     VSTDIEPRFYSIASSEEDELISVLYTEKPDGKLTPMLSMLNPGNPILVSKPFGAFTSF
                     KSEPVFVAAGTGVAPFISALLSNKVRGATLIHGAPYPEYFYFSDYLSEKLGRNYIQCC
                     SRCMPGSHYVGRVTSYLLQWDELDPNRNYYLCGSTEMVVETRSLLISRGVPPDRINAE
                     IYF"
     gene            complement(35088..35798)
                     /locus_tag="PK502_01210"
     CDS             complement(35088..35798)
                     /locus_tag="PK502_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPG99466.1"
                     /translation="MNKADIANYFKGRVIIFLFFILLVSEGIKAQPYVGLRAAYTTST
                     MTFKPDAETKLLTGSGLDYGLEFKYFNAKYVGFQSELYLTSRGYLKAIDVEELGDTLF
                     KRVNLYLELPMFIQFRLKLGIVMLHVNAGPYFAYLLSAKEGDNSTGKYRLNKVDINIL
                     RENRFDYGLMGGVGLSHDFQWGTIQAEARFGYGLGDLYDYSYSGMPSESRAIIQNFSL
                     SYLYRFGQHNTQQEVKQP"
     gene            complement(35929..36228)
                     /locus_tag="PK502_01215"
     CDS             complement(35929..36228)
                     /locus_tag="PK502_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HPG99467.1"
                     /translation="MSVLMNFAMFPTDKGASVSPYVARVVEGVRSLNHASQLTAMGTI
                     VETSSLEEALQVVEKAYQLLEPDCDRVYVTVSLDIRKGGSGRISLKVKSVEDKLA"
     gene            complement(36236..37972)
                     /locus_tag="PK502_01220"
     CDS             complement(36236..37972)
                     /locus_tag="PK502_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPG99468.1"
                     /translation="MLKKILLITIFAVALLSLFPNCAKVVTPTGGPKDTIPPALVNSI
                     PKPNAINFRGEKLVLEFDEFLALKDVQQKLLVSPPLNNRPQIKPKGKRIELEIKDTLL
                     ENTTYTVYFGDAIRDNNENNAIPNFEFAFSTGPTIDTASLAGTIVNSFTNEPVEGALI
                     MLYSSFADSLPYIKRPTHMAKSGKNGRFKVNNLKNIDYKLVAITDANGNYLYNQGIED
                     IAFVEDKVTHDMLMNASDSSRWVRLRTFSEPLPYQLLTGTNRPQRDLIELNFSRKPVG
                     GFKLVQVDNPVSEKWYIPEPDNEGDTVKLWIISEKISAADTLKLALSYLKTDSLNMLQ
                     PRIDTLRFVYYESEEAGNDSRSKRGKKEEVVKPTGFKVETSIKNMAVAIPGVPFEFTL
                     PSPARKVNGALVQIFNQTDSILEPGVKLVADSINPRIYRFNTSWKPNVTYKMIVLPGA
                     FESYTSTLNDTLKLQLTGADPESFGTLTAKFSGVSKAVVAELLTEKDAVVDRKAVVGN
                     QSVTFTFIAPAKYKLRFIDDLNANGTWDSGNYLKRIQPERVIPFTEGKNKGVINVRAN
                     WDNEISVSLQKP"
     gene            38065..38550
                     /locus_tag="PK502_01225"
     CDS             38065..38550
                     /locus_tag="PK502_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99469.1"
                     /translation="MAVAGFKTLHLLIPYIMDNKNFEDNLFFYWTKNLVGTNSRFILN
                     LAIAILFGTLYSFKIAQTNVILLIFGVVSPVIFTLCLYNLILLVSGDKQEVLNFPSVF
                     LVKKSNRLLSIFDSSLVVLLGWLIYRGTLNYFFFRFLLTFFIPVLLIIFLRGLYFFIT
                     G"
BASE COUNT        10638 a         8971 c         8752 g        10357 t
ORIGIN      
        1 atgcaaggtt tttgcatggc tctttggaaa agccacgact tgcctcgcca acacaccctc
       61 tccacacttt taatttcaaa actattccgc cccttcaggg cttgtaaaaa ttgttcgctc
      121 catacaaggg gctgcgcccc ttgctactta ttacgcccct tcggggctaa accaccagac
      181 aggttctgga atccgccagg agccggacaa gttccgccag taggcggaca agttcagccg
      241 gtaggcgaac aagttcttga atcttgaatc ctgtttgatt ttttatcctt tatcctttaa
      301 cctttatcct tgtattaccc tttacccttt atccttacat ttcattctaa ccatttttat
      361 atatatttgc tgaaacctaa aactacttag ttatggaaga aaaaaaatca aacctaaacg
      421 ttacaatgac gtggggttta gtcgtaggac tagcgttgat cattctgagc cttgtacttt
      481 acatcatgaa tatttacaaa gcccctacgt gggtgggtat tctgaactat gcgctgatca
      541 ttggcggaat agtaatggct cagctaaagt acagaaataa cattctggat ggttacatta
      601 cctatgggaa agcagtggga actggagttc tggtttcgct cttcgcatcc atcatctatg
      661 ccttctactt cattattctc acaaagttta tagataccgg ctacatggac aaggttcttg
      721 aaaccgtgga gcaggcatac gctgaaaatg gcctgagcga agatcagatt gcggcagcgt
      781 tggagatgtc gaaaaaaatg atgacaccga taatgatggc cttaagcact gtttttggct
      841 ttgtgtttat gggtaccctc ttctcgctga ttacctccat ttttattaag aaggaagccc
      901 cgcttttcgg cagcgagagc aaggagggtg aaactctttc cgaatagccc tgaattaatg
      961 gacatatcta ttgtagttcc gctttacaac gaggaggaat cgctgtccga acttgcagca
     1021 tggattaacc gagtgatgac cctgcacaac ctgagccatg aggtgattat ggttgatgat
     1081 ggcagcagcg acaggagctg ggcggttata caggagctcc gaaagcagta ccccgccatt
     1141 aggggaatca ggttcaggcg gaactacggt aaatcggctg cactccaggt tggctttacc
     1201 gcagcatcgg gtgacgtggt aatcaccatg gatgccgacc ttcaggacag tcccgacgaa
     1261 atccccgagc ttttcaggat gattaaggag gagcagcttg acctggtctc gggttggaaa
     1321 aagaaacgct acgaccccct gctaaccaag cgaattccca gcaagctgta caactttact
     1381 gtcagaatgg tgagcggaat aaagctgcac gacatgaact gcggcctaaa ggcataccgc
     1441 aacaacgtgg tgaagagcgt ggaggtgtac ggcgaaatgc accgctacat ccccgttctt
     1501 gccaagcagg cgggttttcg caaaattggc gaaaaaattg tgcagcacca cgagcgcaag
     1561 tacggctcgt caaaatttgg gtggtcgcga tttatcaacg gctttctcga cctgctctcg
     1621 gttacctttg tctcccgctt tggcaagaaa cccatgcact ttttcggaac actgggcacg
     1681 ctcatgtttt ttgccggcgg ggtgatagcc gttgtactgg tggccaacaa aatatggtcg
     1741 caaacccaca tgctgaggta cagaccagta actgaccagc ccctgttcta cattgccctg
     1801 gctatagcca ttataggagt tcaacttttc ctggctggct tcatcggcga gttggtgtcc
     1861 cgcagcgcct ccgacaggaa cacctacatt attgaggagg atactggggc ataaaaaaga
     1921 tgagccgcag cggactcgtg taatatgaat gtccaagaac atgaattttt ttgaaccatt
     1981 ttgtttcaaa attagttcct ttggttcatt aaaactcata ccaaaatgaa agcactccat
     2041 tcccatcgtt ccatacgcag gtataagagc actccgattg acgaatcgct gcttaacgaa
     2101 atccttcagg cgggtattcg cgcctcaaca actggcaaca tgcaggttta cagtattata
     2161 gtaaccaaaa gccaggaagt aaaacaacag cttcttccct gccactttaa ccagcaaatg
     2221 gttgtagagg ctccggttgt gctgaccttt tgtgctgatt tcaaccggtt caacaaatgg
     2281 tgcgggcaga gaaaggctga acccggatac gataacttca tctcgttttt caccggtgca
     2341 atagacgctt tgctggtggc acaaaatgta tgcattgccg ccgaggagaa cggccttggt
     2401 atttgctacc tgggtaccac cacctacacc gccgaccgca tcgtcgaagt actgaagctg
     2461 ccaaagggag ttgtccccat tactacggtt gtaatgggct accccgacga gtcgccggag
     2521 ctaaccgatc gtttaccctt agccggagta gttcaccatg aggtttacca ggactactcg
     2581 gagggcgaca tagaccaaat ttacatgaac aaggaatcgc tgcagctgac taaaaagcta
     2641 cttgaggaaa atggtaagga aaccctggcc caaatattta ccgatattcg ctacacccga
     2701 gccgacaacc tcctattttc gaaagtattt cttaagacca tcgagcagca agggtttatg
     2761 aacaacaagt aggattgggt ttatatgcag ctacacactt ttacttcaac acaactctag
     2821 gagaaaaagc atgaagactt ttaagactat tttactggtg ctggtgctgc tcctgatagg
     2881 tgcaggggct ttcctctatt ttggagttta tgaaaagggt gttatggccg gccgaatttt
     2941 aagggtgagc caaaagggtg ttatttttaa gacctacgag gcgaagctca gcatcgagtc
     3001 gtttggagca ttacgaagca ccagccctat tgccgaaacg ttcgatttct cagttgagcg
     3061 caagcaaaag gagctaatca aacagcttga ggaggttgcc ctcactggcg aaagggtcaa
     3121 cgtgcattac cttaaacgct acatgcgtgt tccctggcgc ggcaacacca agtattttgc
     3181 agaaagcata gagcgtttgg ggaagacgca acaaccctaa atcgtgtgtt gaagacaaca
     3241 aaccaagctt acgccaacaa ctaccaggaa aaacaatcgc cctggaaaag ttatatatta
     3301 aaaaaggctg tcggttaagg acagcctttt aagttatcta cgtaaggatt agtaatccct
     3361 tctttcacca cggttaccgc ttccgccacg gctaccacgg tcgaaaccac cccgacgctc
     3421 acggttaaaa ccgcccccat tacggggctt gtcagttttt ggtttggccg gtgaaacgct
     3481 gattaccttt ccatcatact ctgctcccgt taattcgtca atagcccttt gggcctcttc
     3541 gttgttggcc atttcaacaa agccaaaacc tctagaacgc ccagtttccc tgtcggtgat
     3601 caccttggct gatgtaatct cgccatactc ttcaaaaagt tgccttaggt cgtcgtcatt
     3661 aattctgtag ctcagacctg acacgtaaat gttcataaaa ttaaattgaa ttaagtgata
     3721 aattgattgt ttttcgagcc ttaaggcaat acaggaaact tttgcactca cgcacgttct
     3781 gaggtgtggg taaaggattc gtattgtaac ttaattttcg attcggcaca aaggtagcta
     3841 tttttttgaa agccaacatc tttttatttt tcaaaaaaaa aatttggaga aatagtaagg
     3901 ctgcccgaag tgggcagcct tactatttca tgggtattca ccatttcagg ccataataat
     3961 tttttgggta gttgggtatg aaagtatttt caacctcccc tatacattcc ctgtggcttt
     4021 ttcgagcctc ttcataacgg agaagatgaa tatgcccgaa attacgcagc aaataatgag
     4081 aattacccag aagccaacca gtggaagttt ttcccagaaa gtgccgatta cccctaccag
     4141 caaattgccg atagcggtag ctgcaagcca tccaccctgc ataagaccgc tgtactgtgg
     4201 cggtgcaact ttcgatacaa aggataaccc catggggctg aggaatagct cggctacagt
     4261 taacacgaaa taggtgctta tgagccaggc tggagacacc agctgatcgg aaacacccga
     4321 aatttcggag ggtgcaggga gtccgaaaga gcagattagc ataatggtaa aaccaatggc
     4381 tgccagaaac ataccgatac caattttccg gggagccgaa ggttcttttt tcttcttacc
     4441 taaatagctg aacagcgcaa cggttaacgg ggttaggatg acgatgaaca tggggttaaa
     4501 ctgctggaaa atctgcgggg taatcttagt cattacctca attcccttaa cgcgggtaaa
     4561 gtagtagcct gccaatccgg caatacccaa caccgttagt aaaagtcctg attttttatt
     4621 gggtttacca cccagcaatc cgattgtaga aaggtaaatt ccataaagga aaacaataag
     4681 cgatagcaat gggaacaggt taaacagcat agtccaaccg ggcgagatta cgctctgggt
     4741 atagtcgcgc gcaaagaagg ttagcgttaa cccgttttga tggaacgaca tccagaaaaa
     4801 gattactacg aaaaatacaa gcagcaacgc cacggttcgt tcccgaacct cctccttcga
     4861 catcacctta acctccttgc cgttggcctt tgattcggct atttcctgct taactgtttt
     4921 atcaaccgat ttccacgatt tcttgaaaag caggaatatg agtacagaga taatcaggct
     4981 gatgcaggcc acgccaaagg ccaggttgta ggatgtagat agcgattcgc taacgtagtg
     5041 tttaccaaag ttacgggcaa aatcgtcggg attgggacct ccttccagcg ccggatcggt
     5101 aatgctgatt gcctgaattt tggcagtaac ctcagctttt ttcagcatat cctcctggct
     5161 acggctttgc agcagctcat actcgttgtt gctaatcatc atagccggat cggtaatttt
     5221 gcccgactgg gtgtactggt tcagcccagc agccttcaga cggaaaagcg catgctcaat
     5281 atcctcggtg aaaaccacct tgtgcttctt tttggcttta gtaagcactg catcaaactc
     5341 cttaccggag agctgctgct cggccggtgt aaggttttgt acaataaagg tgtttttatc
     5401 tactgcttgc ttctcatcgt tcagaacaac ataattttta ggaattgagg catcataggt
     5461 gaaatcggac gatttcatca cggcattgtt gatgtagtta gcagcacttg gggcaaaaaa
     5521 ggcgccaata ttgatgaaca tgtaaaaaat ggaaaaggca atatcccggt tggtgtcgta
     5581 acgcttgtcg tcgtaaagtt tacctaccag cgcctgtaaa tttcctttga agaatccggt
     5641 acccagcgca atcaaaacga gcgagataat catcatagtc agccccacag aattgccttc
     5701 gagtggaata gccaaaagta aataccctat aaacatgacc aaaataccca gcaaaatggt
     5761 cttcccgtag cccagcacct tatccgccag aactccacca aaaatgggta aaaaatacac
     5821 agaagccaga aaagctccat aaatcatgct ggttgacgct gctgttaacc caaatttagc
     5881 ttgcatgtat aaagtaagga tagccagcat ggtgtagtat ccaaaccttt cgcccatgtt
     5941 cgttagggct aagaccagta aacctttagg atggttttta aacatttctt agaatttatt
     6001 aggttattga tttggttttt ttttgatttg acaaaaatag ttttttttgg tttagttcaa
     6061 aattaatatg ttttatagca ccttccttac tacaaccatc aagcaataca catgatagtt
     6121 gaacattaat ttcagccagg taaacatcaa cttcacttca actttgagaa ataaattgtg
     6181 tgaatactta aagttttagc tttctttaaa tggtcggtac aaattttagc tatactttcg
     6241 tcaaaaatta aaccacaggc tgttagcagc atcctagttg cagccgggtt aagcaacaca
     6301 aaaaaacagt agaaaggaac tttgaccaac cataatggtg agctgaagtt ccgaggggat
     6361 gttcaatatt gttgtttgcc cagcgggaag gggtgcagga taaattttga ttttacacgt
     6421 tttttgccat acgtggcgct tcagggtaat ttcatgcacc ttttaatatt ttgatgcttc
     6481 atgtatagct attcgggtta aaatcagcat taactggtac aacttagtgg tatttacatg
     6541 taagattgtt gctagggcca atttggttaa aaaatgagca ttaaacactc aaaataccca
     6601 aattttgatg atgtaatgtt tatccaacaa gctaaactta aaaacatacc ctggccaaat
     6661 tctttccgtg acagaacatt acctctgaaa cctgaatgaa accaaaccac cggcaggaat
     6721 atacgtatgg agtacacatg tcaataccaa caccaacatg tatgaaaagg tcatacctca
     6781 acctacgatt attttgtttg ttaaaaaagt tacagctgaa ttacaattac cataaacatc
     6841 tataaatacc ctaaatatcg ataacggatg aaaaagatag ttctttttgc cctggcaatg
     6901 gaggtgcttt caggtatggc tcaggcccaa gatccttgga cactggagca atgcattcag
     6961 tatgccaacg agaataacct gcaaataaaa atgggtgaac tctccgtaaa catgagtaag
     7021 gataagctgc tacagtcgaa actcagcatc taccctagcg caaacctgaa tgccaaccac
     7081 gcctacagct ttggacgagt tacgaactac atcaccaacc agaaggagag taggaatacc
     7141 caatctacaa gtttttcgtt tagtagcagc atgcccttgt ttgaggggtt tcagcttacc
     7201 aacaccaaga agcagtccaa ctacgatttg ttggccagca tacaggatgt ggagactgtg
     7261 aaaaacaata ttgccctgtc ggttgcctcg gcctatctgc aaattcttta ccagcaggag
     7321 ctggtagaag tggcacaacg tcaggtagag caatcagaaa tgtaggttaa acgaaccgcc
     7381 cttctggtaa atgcaggaag ccttcccgaa ggcaatctat acgaggtaga ggctctccta
     7441 gcttccgacg agctgcagct ggtcaattcc cgcaaccagc tcgacctggc ataccttaac
     7501 cttacccagc tacttgagat taaagatgtt gagaatttca gaatagccaa accaaacatt
     7561 tcggaggttg acagcattgc tgttatccaa aatccacagg aaatttttac ggtagcccaa
     7621 caaaccatgc cccaaatact cggttcacag tataaggtgc agagctccga aatgggctta
     7681 aaaattgcca aaggagcaaa ctacccacgc cttactctta gcggcagcta cagctccgga
     7741 gctcagagct acctgaacaa cgacaaccca ctgctcactt ccgatccatt tttcacccaa
     7801 ataaaggata atgcaagcac ctcggtgggg cttagccttg gaattccaat atttaatggc
     7861 ggaagcaccc ggtatagggt atcaacagcc aggatatcgc tcgataacgc ccgcctaact
     7921 ctggaaaatg agaaaaacat tctctacaag gatatccagc aggcatacgc cgatgccata
     7981 gccgcacaga aaaaacataa ggccagccag aaaagtaaaa tggcctccga ggaatcgttc
     8041 cgctacaccg agaataagtt caaccttggt ttgctgccag ccatagacta tacaacagcc
     8101 cgtaacaacc tggccaaggc cgaaaccgac ctgctacagg ctaaatacga gcttgttttt
     8161 aaaaccaaaa ttctcgactt ttacaaaggt gttcctttga gcctttaagt ttaattcgta
     8221 ataaaacatg tacattttgg cttgggaatc agcagcaacc aattaaggtt ttaccgtttt
     8281 aaaatatcga catacttttg ataatttgat gtttaactaa accatactct cgtgaaaaag
     8341 ggattactga aatacctgat tggcgcaatg gtcatcataa tagttttagc cattgttggt
     8401 aaaaaaaagg gctggttcgg ctcaaccgat gctattgagg tttatgtaga gaaaccccaa
     8461 aaaaggacta ttaccgagtc aatcaccgct aacgggaaaa tacaacccga agttgaaatt
     8521 aagattagct ccgaggtatc tggagagata atagaacttc cggtaaagga gggcaactgg
     8581 gttgaaaagg gaacgctgct ggtcagaatt aagcccgata cttacatctc catgagggat
     8641 cgtgcaatag cctcggtgaa ctctgccaat gcacgtttag ctcaggccaa agcccagctt
     8701 agccagagcg agctggcttt taaccgtagc aagcagctgt tcgaacaaaa ggccattgca
     8761 gaatcggaat ttgaaaccgc caaaaccaac tacgaggtgg ccaaatcgga actcaaggct
     8821 tccgagttca atgtggaaag cgcccaggca tcgctgaagg aagccgagga aaacctccgc
     8881 aaaacatcca tatactcacc catatcgggc accgtatcga agctaaacgt agagctgggc
     8941 gaacgggtgt tgggaaccat tcaaatggca ggaaccgaac tcatgcggat tgccaaccta
     9001 aaccggatgg aggccagggt tgatgttaac gagaatgata ttgtaaaggt gaatattggc
     9061 gacaccgctc tggtggaggt tgatgcatac ctggatcgca agtttaaagg gatagttacc
     9121 caaatcgcca acactgcaaa tgttcagggg gtctcaaccg accaggttac caactttgag
     9181 gttagaatat tcctcctgga tgactcatat aaagacctgg ttaccgattc ccggcttagc
     9241 cctttccgcc cgggcatgtc aaccaccgtt gaaattctta ccgagacccg tagcaacgtt
     9301 ctcaccgtac ccatactagc ggttacagtg cgaagcgatt caaccgccaa gaaggatatc
     9361 accggaatgg aaaaggttga cgattcgtca acccccatcg aacaaaccaa ctcgaagctg
     9421 aacaaaccta aagaaattgt atttgctgtt tctggcgata cagccaggat tatcacggtg
     9481 aaaaccggaa tacagaacaa ccagtacatc gaaattgttg agggattaaa gggtgatgag
     9541 gatgtggtta cggctcccta cagcgcaatc tcacgcaagc taaaggacaa atcgctggtt
     9601 aaaaaggtta agaccaaaaa tgatctcctg aaagctgagg ttgcaaagaa atagcattcg
     9661 ggttgaccgt tattcctaac ccggtatcta cccaatctgc tgccaggctt ggcttggcag
     9721 taagttgtgg atggctcagc aaacaggtgg tagggtttaa tgggcttttc acccctgaaa
     9781 cccaacgttt ttgagttaag ccaacaggcc ctcatcaacc tgaaatgaaa aaataggaat
     9841 tgccggggtt gcagacaaaa aattgctgta gcaaccctga agaattagct tcgcacaatt
     9901 gcaacaccaa catggcaacg attctttgca tggaaaccgg ctcaacaacc tgctcggtag
     9961 cactgggcag cgagagcggc gttattggat tcagagaggt ttccgatgcc aatatccatg
    10021 cctcggcgct tcctgttctg attgaagagc tgataaggga aacgggtttt gaattaggga
    10081 atattgatgc cattgctgtt agcatgggtc ctggctccta caccggcctg cgtataggtg
    10141 tttcgctcgc caagggaatg tgctatgctc tgcataagcc tctaatcgct gtgggtagcc
    10201 tggaggcaat ggtaaccggg gttggagaat ttatcaacat ggaaagcatc aacaacaact
    10261 ctctgctcag ccccatgata gatgcccgac gaatggaggt ttacacccaa ctttttaaca
    10321 ggaagggaga aatgctgtcg gaggtaaaag ccttggtggt ggaaacggat actttcagcg
    10381 atttgctaaa aaaaaatact gtcgtatttt tcgggagcgg agcatccaag tgtcaacagg
    10441 tgattggtaa cagtggtgca atatttgttg aacatttcaa accatcagct cgctatttac
    10501 tacctttggc gcaaaagaaa tttagagatg aattgtttga ggacattgca tattttgagc
    10561 catactacct taaggacttt gtgcccacaa ggccaaaaaa caggatcatt ccggattaac
    10621 aagaattttg aactacttcg atcccgccaa ggattcgttg tactgctggc ctgcattcta
    10681 attctaggca taccggtagc ttcggctcaa aaggcatttg gaccgggtga aaagttaaac
    10741 tacaaaattt actacgggct tatacatggc ggcgaggctg tgctcgaggt gaggaagggc
    10801 gactacaagg gaaagcctgc caatcatatt tacatggccg gcaaaacggt tggcctggcc
    10861 aacaagtttt actacgttga ggatatctac gagagctaca ccgaccccga aaccgactgg
    10921 ccatacttct cgattaggaa tattcacgag aaccgttacc ggcactacag cacccaggtt
    10981 tgggatcact ggagccgtaa ggactcaagc atagtcaaca gctccaaaac aggagaagtg
    11041 gtagtggtaa agggatgtca ggatatactc tcgtcgttct actaccttcg taatatcctg
    11101 ataaaatcag atcctgtgaa ggatcagctc tttatcgttg acacctactt taccgatgaa
    11161 aaatttccgc taatggttcg ctttaagggt cgggaaacca ttaaaacaag tcttggagaa
    11221 tttcgatgcc tgaaatttat gccggttgta gaggtaggac gagtttttaa aacccaggac
    11281 gatatgacaa tctggctctc ggacgacgat aacctggtgc cggttagagt gcgcttcgag
    11341 atttttgtag gagccttcta ctgcgattta tacagttttt ccggtttaaa aaatccgttt
    11401 tcctcctata gtcccaaagt gaaataattt tcttaccccg gcaatagagc actaagttgt
    11461 ttttatttaa acccaaacat gctgcttgtt ctttgtgccc gaatgctcca tggagaaatg
    11521 cttacttgta aatttgtttc aacaagctaa agaattatta catttgcacc ctaatttgtt
    11581 aaaaatattt tatggcaaca acggcagatt tcaaaaacgg gctatgcatt gagtttaacg
    11641 gcaagttata caccatagtg caatttcagc acgtaaaacc gggcaagggg ggagcattcg
    11701 ttcgaactaa gcttaaaaat cttgaaacag gcaaggtaat tgaaaataca tttacctcag
    11761 gggtaaaaat aaatgttgtg cgggttgagc gtcgtcccta ccagttcctc tataaggatg
    11821 accttggttt tcacttcatg caccaggaaa cctttgaaca ggttagcctg agcggcgacc
    11881 tcatcgaaaa ctccgacctg cttaaggaag gacaatacgt tgaaatgatg gtagaagccg
    11941 ataaggagaa catactaacc tgcgagcttc ccccgtttgt ggagatggag gttacctaca
    12001 ccgaaccagg cctcaagggc gacaccgctt cttcaactgc cctgaaaccc gcaaccgttg
    12061 aaaccggttc ggtgatcaac gttcccttat ttgtcaatac aggagataaa atccgtatag
    12121 acacaagaac aagggagtac tacgaaaggg tgaagtagtt ttcatcgcta tccaagctaa
    12181 attattcata aaattttgac ttcccctttt tttattgtta acttattcca cttttccaag
    12241 ttggattggg ctactttttt aaaccatgtc tatagcaacc tcgcaaaata ggttaaagtt
    12301 aagcacattc aagctaaatg ccctacttgg tatggcccag gcaattagcg ccgagttgaa
    12361 aaccgaggag ctggttgaca ggtttagatc aatccttaac gacgatcttg gaatagacag
    12421 gatactactc tacaaaaaag aggaggatcg ctgggagttg cttctaaaca gcaactgccc
    12481 gcaaaaagtg gttgagggga tatcggttga gcgcgacttg atggccttta acgaaataac
    12541 ctttgttgga gtagccgaaa accccctgct attggaactt gatgtaatca ttcccatcgt
    12601 tcaaaacaat gccccggtgg gttacgtcct gattggcgat accagcgagt acgaaaaagg
    12661 ggtaagcgcc accatcaggc acctcaactt tgtgcaaacc ctttccatca ttattttcgt
    12721 ggcaatagag aaccttaggc ttttccataa aagtttggag caagaggcca tgcggaagga
    12781 acttgagctt gcctccaaga tgcagtccat gctaattcct accccggagt ccatgccaca
    12841 ctacccccac atttccattg cctcattcta caacccacat tttgaggtag gcggcgacta
    12901 ctacgatgtt atccccctaa acgataatga attcggcttt tgcattgcag atgtttcagg
    12961 caagggaatt tccgcagccc tgctcatgtc gaactttcag gccaacctgc gggcactttt
    13021 ttcactggat atccccctta caagattagt tgaaaagcta aacgaaaggg ttatggctac
    13081 tgccaatggc gaaaaattca ttaccctatt tgttgggcga tacaacgttg agaagcacct
    13141 cctggagtac gtcaacgccg ggcacaatcc acccttcatg attgacaagc aaagcaaaat
    13201 aatttcaacc ctttccaacg gctgtgtggg cttgggaatg cttgactgca ttccggttat
    13261 taatcaggga gccgttacca ttgaccagcc gtccctgctg gtttgctaca ctgatggcct
    13321 ggtggaaatt atgcgcaaca acaaagtgga gtacaacaca caggtaatcg agcaggaaat
    13381 tgttaagctt gagcccatat caaccacaat tcagcgtata gccgagtcgc gtggcattaa
    13441 gaacgacggc agcagcaacc ccgaaatttt tgatgacatt tcaattttag gctttgggtt
    13501 caactgctgc tagtcctata cgctgtattc atagcagcta tcatagccag atatccccaa
    13561 aaaagggctg ctattttgtc catatccaaa aagttgttca ggaacccgtg aaaagcatag
    13621 gttgacagcc ccagcaacac cccgagcaat aacttccggt agggaaaatc cagcggcagc
    13681 ttacccagca acctaaaccc cataataaaa gcaactaata gaattgcgat atatcccata
    13741 gtggcaggcc agcccgcctc ggcaagcaaa cccaggtact cgctatgggc attccccttt
    13801 gcccccaggt ttgaactttc aacggtaaaa aattgcgaag gctgaaatgg ggcataggtg
    13861 aactggtatg taccaggccc ccaacccaaa aacggacgct ccctaaacat gcgcaatgcg
    13921 ctggtccaac ggtttagccg ctcaacgttc gacacgtctg ttgtaatatt tccaacagaa
    13981 agcagctgat cgcttaggct gcccgttgat accgtctggt ttttccccac ccattgggcc
    14041 agctgacctc ttaacccaaa acctattgca ataatactta ccgaaaggat ggctatagta
    14101 cgaaatcgca gcttcataag ccatactacc catataagta aagctaccaa aaggctaagc
    14161 catgccgccc ggctatagga tagcaccagc gcaacggtaa aaacgaggat aagtattccc
    14221 attaatattt tctgccatag cgtttttgtg ctggcggccc aagccaccac aacaggaatg
    14281 agaaacgcca acgctgcacc gtaggaggtg tggtcggtgt agaatggatt acaggttcca
    14341 accgcagcaa acttatcaaa aatgcctatt ctagcctgcc ggtatagggt tatagccaca
    14401 actaccgtca atccggttat gtagaaggca atgtactttg taaagttctg aggcgttgcc
    14461 ttaaacagta caaatggaat gtagaagaaa aatgctatgt aaccaattcg cagaacagtg
    14521 tactttaccg ataccaaagg taactcgctg ctgatacatg ttatgacaag ccatccaagg
    14581 tagaagagca tggcccagaa gagtggatta ccgaaaaggc cagtgtcaaa tcggcgctcc
    14641 tgaataagat gaatagcggt taacagaagt agagcaaaaa tgaggggctc ggttggcagc
    14701 cacaggtcga aagaacggcc tggcaggaac tcgctaagag ggacggatag cggcgccagg
    14761 gcaattacca ggtaaaatat ttttacgggg acaagcacta ctcccagcac caacagcagg
    14821 ataaccggca gcaggctgaa cgacagtatg tttttagtaa ccagtaggct gtttatggct
    14881 acaaaagcaa gaccgagaaa cagtgcagcc agctttgttt tgtgctcatc ggttaccatg
    14941 gccacgggat tagtcgcctt ggtttaactt tgaccctctt gccagctcaa gaactgcaac
    15001 cacaaaaatg gtaaaaaaaa ttgcagatag cgtggatatt accactatca gccccctgtt
    15061 tggggaaact tttacgctag gcttaacagc gcggtcaatt acgtagcatg tgggtatatc
    15121 acctgtgacc tgcacacggg caaacgcaat ggcctgcctt aaccccgata actccacggc
    15181 aggggctaac ccaaaacttt ttttgagatt agcgaaagaa tcctgtaaac ttgccagctc
    15241 catcaacgcc agccggtact gatgttcaag cgcctgaagg gttttagctg ccacctccga
    15301 ttttaaggag cgcatcatgc tgtctgctat accaacggct gcattggcaa ctgctgcggc
    15361 catttgcgga tcggcatcag taacctcaac cgctataccg ataaattggg taggcttaac
    15421 ccttacatgc ttgccataga ggttgcgagc tgcctgcatg gatttatcgc ctgttgtctg
    15481 caacccccag tagctgtgaa gacccattcg ggagattagc gtatccctaa aagcaccgga
    15541 ggccatcacc tcaagaaact gctctctttc aacggctccc ccaaagttgt taaattcaac
    15601 gaacaggcta tccgacaatg gagtgcctgg tacctgtagc tcccttgtag gagcaaatac
    15661 cgcagtagca cggaacttcg gggctattaa caaagaggca acggttgagc ctaccagtgc
    15721 tgctacgcca acaataagaa ataccaaacg gtaacgcttc caaaccttta ccgttgccac
    15781 gaaacttggt tctgcagttc tataatcctg ctccatgggt tagggttgat gagaactcga
    15841 aaatttaaaa tgctaaagtt ccatattcgg cattaataat agatactgtc ccaaaaagtg
    15901 tgtaaagcca ggctttcata attttgaagc gtaaactttt aaaaagatga aaaacctaaa
    15961 ctttacacgc gggcaagtta cgaacatttt agaaggaatt gccaaaaagg aagaaggcgt
    16021 acagcatatt ttaaagatga gcatagaggc aattaaaatg gtctggcggg atgtttacaa
    16081 caaacggcac caagatatta gcaattccat gaagatgcaa attctgccat tgtttttgca
    16141 cttggcttta ggcaatacca cgtatggtag ggagggcgcc atttaacccg tcgtaggttg
    16201 gactaggcct ggaagatagc accaatcaaa aatacccggc ttttcttcgc tcttactatc
    16261 tttgacaaat tcggagcatg cgctgccttt gcttaatcaa gcttacagga aaaacgtcct
    16321 aacccgtttt aagtaaagtg tcaaaaaaat agggggttaa accttatacc gtacgtatta
    16381 tgctgtagct ctttttgccc ttctgaatga ggatgtactt gccatttagc agatggctgt
    16441 tggaaaccgg aagctccgtg ttttcaaccc ttaccttatt caggctaagt ccaccgccgg
    16501 taaccagacg ccgtagttct ccttttgatg agaaaatggg tgccaactca acggcaagcg
    16561 ttgaaagggg cactccttca gcaatatgct gccggctaac ctcaaataat ggcacaccct
    16621 caaaaacgct aaggaacgta tcctcatcca tggacatcag ggtttcggtg gtggcattac
    16681 caaatagcaa ctcggaggcc tccacggcct ttcggtaatc ctcctcggag tgcaccataa
    16741 tggtaatttc gcgtgctagc attttttgca gctttcgcaa atggggttcc ctacggtgct
    16801 cctcaacaag ggcatcaacg gttgactggt cgagcagggt gaaaaccttt atgtactttt
    16861 cggcatcgtc gtcggaggtg ttgagccaga actggtagaa cttatagggc gacgtaagtt
    16921 ttgagtcaag ccacacgttc ccgctttcgg tttttccaaa cttgctcccg tcggccttgg
    16981 taatcaaggg gatggtaagt gcaaaagctt cgccaccggt catccggcgg atgagctcaa
    17041 cccccgtggt gatgttaccc cactggtcgg aaccacccat ctgcagacgg caacccaggt
    17101 tcttgtagag ccaaagataa tcgtaggcct gtaccagttg gtaggaaaac tcggtgaacg
    17161 acatgccctc cttcgtttcc tcgccaaatc gcttctttac cgagtccttt gccatcatgt
    17221 agttaacggt gatatgcttg ccaacatcgc ggatgaaatc gaggaacgag aaattcttca
    17281 tccagtcgta gttgttcacc ataacagctg cgttggggtt accgctgtcg aagtcgagaa
    17341 accgggaaag ctgattccgg ataccctcca ggtttttctg tagggtggcg ctgtccagca
    17401 ggtttcgctc ctgcgacttg ccgctggggt cgccaatcat tccggtagcg cctccaacca
    17461 gcacaatggg cttatgcccg catagctgaa acctcttcag cagcatgatc tgggcgaagc
    17521 tgcccacatg tagagactcc gcagtggggt cgaatcctat atatcccgag gtcatctcct
    17581 tattcagctg atcggccgtt cccggcatca cgtcgtgaac cagtccgcgc cattggagct
    17641 catctatgaa attcatttct attgtaaaat tagtccggtg caggaatatc aaaaaaacgt
    17701 ccaaatataa gcgtttttgt cccaaaagta actacagaaa gtcgagtgta ggtgaaaagg
    17761 aaggtcgaac ctacttaccc ttctggttcc cgagggattt tttaaccgat tccttaattg
    17821 tgtcgaaatg aaggtagggg aatattgccg gagcaaggct tgcaacaggt ttgtacacca
    17881 gcgattttga tttttgctcc tcggtgaaga agttgaaacg tatattagcc ttttcaacaa
    17941 aaaccagcag cacgctggta ataatagcgt acttggcaaa gccgaaaaca aacccaagca
    18001 ctttattaat tccccccagc atggccaaat tcaaaaacct ttcaactatc ttttcaataa
    18061 agtggatggc tattacaatt aaaatgaagg ttacggcaaa cgaaatgatg gggagatgag
    18121 ccgagccaat gtcaaacttt tccgaaagca gggtggccat gtagtctgaa aaacgtactg
    18181 caccccaaat acccagtaga agagcaacta ccgatgccag ctgggacagg aatccctgcc
    18241 ttaatcccct tatggcgccc acaaccagga gaattaccac aacaatgtca aatactgcca
    18301 ttagaagggt tgttaggatt agaaccttgt ggaggtgcag ctgcccacac aatccaaaat
    18361 ctttcccaaa ttctcgttac gcagaaagta caggttgttc tttccctcgc gcttgcaggc
    18421 gagaagcccc ttatccttta aaattccaag gtggtgtgag gcggttgact gctctatttg
    18481 gagtttttca tgaatttgcg taaccgtaag cctcccatta gcctccagta agctgatgat
    18541 ggctatgcgc atagggtggg ccattgcctt tagcatgttg gcggcatctt cgagctgctc
    18601 aaccttcaac tcatttatct ttaccattta aaaaatactg ttaatagttc catccgcaca
    18661 aaatttagtg ctataaattt acaattagcc acgctttggc atttctatct tgttaacctt
    18721 cggatgaagc acccattgtt tggattcgcc gaatccgcaa gttacgctcg gttgttcgtc
    18781 cttttttatt caggcttact cacaaaggtg tttcatatta catccatgca aatatattaa
    18841 tattttacta caaacagaat tttatgcccg gcctgtattt accagccaag cctcctcacc
    18901 agcttcgcag aggtatttac ccaattccac attctaccag ttgtcgaata tcaaccccga
    18961 ggaggagcaa gttgaaaggt tagtacaggc gaaaggcact aataaaaaat aagtgtattc
    19021 ggaacttcat gtaataacaa gggaaaatca acaaaaatgg catttgccaa atgtcggcta
    19081 aacttttatt gagctttagc gttaaatgaa taaccaaaaa aagtgttaat ttaccgctta
    19141 atattttcaa cattccagga atgctcgaag ccattaaaca gccagttgac gaggagctga
    19201 aaaaatttga accctttttc aggaagaaca tgggctcaaa cattcgtttc ctcgacctga
    19261 ttaccaacta catataccgc aacaagggga agcagctacg accaatcatt gtatttctat
    19321 cggctgggtt gaacggcaag atttgccagt caacctatgt tgctgccggt atgattgagc
    19381 tgctgcacac tgccacgctt attcatgacg acgttgttga tgagtcgttt gagcggaggg
    19441 gtgctttttc catcaatgcc ctctggaggt cgaaggtagc ggttctggtt ggcgattttt
    19501 tactttccaa aggcttgctg gtggccgttc agaataagga gtacgagttg ttggaggtga
    19561 tgagcaacgc tgttagggag atgagcgagg gggagttgct gcagatagag cactcacgga
    19621 aaatggatat taacgaggag gcttactacg atataatccg taagaagacg gcctacctta
    19681 tagcctcgtg ctgtgagaat ggggcaattt cggctggtgc cgattccgat tccataaggc
    19741 gaatgcacga atttggtatt aacctgggaa tggccttcca gattagggac gatctcttcg
    19801 attaccagcc caaaggcctg gttggtaaac ccaacggcaa cgacatcaag gaaaagaagc
    19861 taaccctacc cttgatttat gcgcttaggg aggcacccgc tgctgaaggc agaaaagtac
    19921 tgcgtgaaat caggagcaga agtaaaagca gcgttaccat ttcaacagtg gtaaagttta
    19981 ccaatgaata tggaggaatt agctatgcca ccggacagat gctaaacttc aagcagcaag
    20041 cccaggaagt gctcaacagc tttcccgatt cggattaccg ccaatcgctg atgcttctca
    20101 ccgattacgt tacggaccgc aacaagtaac ccctaaagtg ttgggaggtt tatcccctta
    20161 atgatcctgt aggtttcgag cgcatacaca tccgtcatcc ccgacacaaa gtcgagtacc
    20221 gacataatct tagtgtagtc atcaccactg gaagtcatgt actggtttgg aacaagccgt
    20281 atgacctttt tggatagccc ggtttgtgga tggagaacgg catcgcagaa aatttcgagc
    20341 agcgacccca ggatttggta gcctgctatt tcaatctcaa ccaccttcct gtggttgtaa
    20401 actctctcca ccgccaggtt tttaatagcc tccatagccg tatggcttgc tcccgatatt
    20461 ttttcgagca acgatgaaaa gtcttctcca tccatgattg gcttcaggtt atccacaaat
    20521 atccgagcac actcctccac cagcttgctg atgacaatag ccctaaggta ggccacctgt
    20581 tcgttgggat cctgaacctc ctttagcgta gaggtaattc tatcccttgc acttttgggc
    20641 tcatcggtaa aaaaagcacg gtagaggtca accaccattg gcgtggaaag gatgcccagc
    20701 ttatgggcat cctcaatatc cataatctgg tagcagatat catcggcagc ctcgacaagg
    20761 taaaccaacg gatggcggca aaattccata ttgcctacac tatgcattcc tgttgcatgt
    20821 gcaatactca gaaagggatt ggcctccgac ttaaaaaaac cgaacttctt tttgcccgaa
    20881 gctgatgggt agggatattt aaccactgaa gccaacacgg cattgctaag cctgaatcca
    20941 cctgtccgcc gcccggttag ctgctgggta aggagcctaa gggcgttggc gttgccctca
    21001 aatcgcacca aatcggacca ttcccagctg tccaccatcg gtttgaaaac cccgctgttg
    21061 tcctcaaaaa actttcgtat ggcctcctca ccgctgtggc caaaaggcgg gttgcccatg
    21121 tcgtgggcca agcatgcagc tgcgatgatg cctggtattt cggcaaccag tggtttttca
    21181 tgtggactaa gggtttcgga tatctgcagg taaacattct gtcccagcga tcggcccacg
    21241 ctcgacacct caaggctatg ggtaaggcgg ttgtgaacaa atatgcttcc cggcaaaggg
    21301 aatacctgtg ttttgttctg caaccgacgg aagggagagg aaaaaataag ccggtcgaag
    21361 tcgtgctggt agttggttcg ggcggtatcg taagtgtttt tagttgcccc ggttgcgcca
    21421 aaccggctgg catcgagtag ttttgtccat tccatttgtt ggcataaatt gactgatgtt
    21481 tggcactacc ttttgggcag aaaaaggaat accttattcg ggaaattcta cctccatttt
    21541 cccataatgc taaaattcgc cccttgccct aaaggattcg ggtaaaggta ggtaaattat
    21601 taaggtagta ttcgcataaa cgctgcacct tatatgatgg gaaaaagttt acgggcggaa
    21661 ggtgctgttt aaagtttcat ttatgcagct tctactcgcc ctcatgcagc aggtggctca
    21721 cgttgactgc aatcttcatg attttaaaaa cttttccgtt ctcgtccttt agtggtgtat
    21781 aggtctcgaa aaagaggtat gttcttttac caacggtgaa ccgatgagtc tctttgcggg
    21841 cgattccgct gtttaaatcg tcccagaact gcttgtagtt tttccgttgc tcatcggtaa
    21901 aatcgagctg gtaggaatgg tgctttccga ccacatcctc ccttttcgag ttgaggagct
    21961 ccaggtaagc gtcgttgata aaggtgatgt acccgtcggg gttgtactct ataacgtagg
    22021 tggagttgtt tattgcctcg tagaggtttt gcgcctctag ggcttttttc tgcatgtcgc
    22081 gctggtatgc aagggcatcc atttcctttt tcttaacctc ttcctccatg agcttcgatg
    22141 cggtaatatc gcgagtaatt ccgaaggtgc caatagtttc tccccttgga ttcttaagag
    22201 gcagcttgga agtttcggcc cacgataccc tgccgtcggt aaacacctcc ttctccacca
    22261 gcccaataat aggggagtcg gttcgtataa tgttctgctc gtcctcgtag gcgggacgcg
    22321 catgctcctc tccaaaaagg tcgaagtcgg ttttgccaat caggtcatcc gggttctcga
    22381 acccgtgggc ttttagggtt gacctgctta ccttgatgaa gcggctcttc aggtctttga
    22441 agtatatttt gtcggggacg tagtttagca gcgagtcgag tagcgatttt tcccactcga
    22501 gctgtttctg gttttcaaga atgtcctcgt tgcgtttttc gagttcctcc tttatggtgg
    22561 caagttcctc caggttctgt cttacctcct cctcctgagc cttcatctcc tctgtttgtt
    22621 gctgggtttt ttcgagcagc tcggcggttt taaggtttgt cttcacggtg gtaagggttt
    22681 gggctatgct ttgagctaac ttttctacaa actctacctg gtagtccatt atggctgaga
    22741 aagaggctat ttctataact cccagaacgt tttcgtcaac cttcatgggc acaataagga
    22801 ggttgcgggg tttggcttct ccaagtccgg aggaaatggt gatgtagttt tctggtattt
    22861 cggtgaggta aatggtttgc ttctcaatgg cacatgttcc gattaaaccc tcgccaggga
    22921 gtatccgttt cttaatgaac ttttgacggt tgtaagcata ggcagccagt aggtcgaaga
    22981 atgggtttga cgggtcgtcg tcgttgtaaa caaacaggcc accctggtta gccccaaggt
    23041 agtcaatcag gttacgaagg atgttgatgg acagcaggtc gagcctatcg ttattctgcc
    23101 ggagaatttc actgaatttt gccagcccct cattagtcca ttgccgcttt tggtcatcag
    23161 ctgctcgttt ttgaacttcc tcctgcgcct ttttaaggtt agccgatagg tctattagcg
    23221 attggccaag ggcatcctca tcgctcatca gctccatttt agctccatag ttaccctgcc
    23281 cgatagtttg ggcaaactca gtttttttgg tgagaccgtc aactgttcta ttcaaatatt
    23341 ccgacatggc ccccagctca tcgctaacct caacatgcac cttcatgctg ccatccacat
    23401 gtccttttgc cagccggcta aggttttcag aaacgtgaat aataggctcc agaatgtatt
    23461 tcctggctac tgcctgtatc agcaaggtga gcaacagaat gcctgccagg ccaaccagca
    23521 gggttttcca aaaaattacg gtggcatcat gcagcacaac accttttgga acaagcaaca
    23581 ccaacgccca gctctggggg caactaccaa ttatgattgg acgaatggcc acaaatgaat
    23641 ttgctccact agcatcggca ccctcaaaaa aggcagagtt gccgttggca ataatttctg
    23701 gtactccatg cagggtaaac aactcctcat agctggccat ggcgtcatca cctgccaagt
    23761 tcttatcggg atgggcaata aacttcaggg tggttgagag cacaaaggca tagctccctt
    23821 catagcggtc aatgcctctg atgagctcgt taaaaccagt gagggggatg tcgacacccg
    23881 acactccggc aaacttccca tcaatgatgt tgggcacaac caggctggtc atcagcacag
    23941 gggagccgtt atcgaaagaa aaaaaatagg gctcctcaac cgattcggtt agctcttttt
    24001 tgattcgggc gtactggggt gaatcaggag taagaatggt ctccgatttg gtgagtattg
    24061 ttccgttgct cctccatgcc atgttcctga ttctaccgta gctgttgctg aaagccgggt
    24121 caagaaattt gagctcccag ctatcccaaa cggataggat attcggttcc gattgcagca
    24181 cgtcccggta agcagaagta aggtagctgt tgagcctatc gggtggcagt tgactgcagg
    24241 atttggcagt tcccgatagg gttctggcaa tatacagatt cttttctagg gcattttgaa
    24301 tcatcaaggc ttgctgatcg gcagtaatgc ctatctgggc aatggcgttt tcaacagatt
    24361 gtctttttag ctgattgctg atgaaaataa gagcagctat aaggataaga atagtggacc
    24421 cggtgaacag gataagaatt ttacttttaa gtgaaagcga tgaaaaaaac tgaaggttga
    24481 gtttccttgc gtttggttta ctcttcatca taacccgatg tgtggtagag attagtaaaa
    24541 actttgatac ttacgaatcc tggggttaac gcacataacg gggatgtcca ggttggcctc
    24601 ccttttggca tcaacaaaat agttgtactt atcggccata actagtatca aatcggcctc
    24661 aatggtattg gcaaagcgga atacctcgtc cttaaaaaaa cttttctttt tagcggtttt
    24721 aatgccgtaa ataattccca caccatcgag catctttttg gtaaagtaga tgttgttggc
    24781 cagctgcttc ttttgtccgt catcggtaag aaacggttta ataaggttga tgttgcactt
    24841 gtagtatttg gccaggtaga tgatccagtg tacagcctcc ttgaacttgc ggtcgtaatc
    24901 aaccgggatt acaatttcga tatagtggtc gtgccttggg ggcttgctta caataataaa
    24961 gggcagggta atatctttaa aggttaaatt cttaatgaaa tcggaggtgc ggtaggtttt
    25021 atccttaatg gtgtagccat gttcggcaac aatcaggttc acgtttgccg tgagtattaa
    25081 ccctctgatt atcttacggg gacttccctc cattaccaag gtttgcggga taattccaaa
    25141 cctgctctgc acatcttgtg caaccttctc gaattgcttg ttgattgcct caattttttt
    25201 gaagttcggt ttgtagaaga tgtcaaagag tggggaatgg ggaatgatat gcagcagcat
    25261 aatttcgtta tctccagcct tcgccagctc aatggcatgt tgaagagcgt attcggaact
    25321 ttgggtgaaa tcccaaggaa caagaactac ctttcttatg ctatccatac cgagatgttt
    25381 tgtttataaa atggcatact cattcatgct caacaattgc gagtaaaagc caaagtgatt
    25441 aattcccatt acatttgaat aaattcagta aaagtaaaat attttgtgta aaaatcatag
    25501 ctaaaggttg gatttttcca tttggtttat ccaactcttg atgttctgaa gctgcatgtc
    25561 cttgtttaca ttccttagcc ttcgtgcggt ttgggtgaag tactggtggt tttgaataaa
    25621 attcatttgc agaatattcc attcgtgaag ggaaccaatc attccatttt cgtgcatctc
    25681 cttgtaaagc atgttggaaa cggggataaa gtccgttcgt ccgaggtaat ccaggtcggc
    25741 atcacatata atttcctcaa gaaggttttt cggattgggg ggtagcttgg ttgacatgat
    25801 tagcttgcag atttcatcta tctggtcgga agagtaccgg tagttgggta gaatttccct
    25861 ggctagcttt acccccattt gctcgtgggt ggcgtagtca accaggtggc ctgcgtcatg
    25921 aaacagggca gcggttcgga gtaggagcag atcctccttg ctaacccctt ctgcacgccc
    25981 aatcagctca acctgggtgt acacatccac cgtatgcttg acgttgtggt agtatagatt
    26041 ttttggaagg ccctgctcaa gtttctccag caccagctct tcaaggtcgc ttagccggat
    26101 tagctggagc tcgatgaaaa agtcctggtt aggaagtgtg ccttcgaggt tttgggatag
    26161 gttgggcaga ataccctcca cgaagtacat ctgtatgttc cccctattct ttacggggat
    26221 ttgcccacgg taggtgcaaa tgaaaaaatc ccgcaccagc aggtaggtat tttccgaaat
    26281 gtttatctta ccagcttcgc cggccgattc catcctgctg gcagtgttaa cggttgctcc
    26341 ccaaatatca tacgaaattt tactttgccc tactactcca gctataaccg gcccggtgtc
    26401 tatgcccata cgtagctccc acacatcacc tttctggtgc cttcggttag tttccttcat
    26461 ccagctcatc atttcaagcg caacaaccac cacttcaatg gggttggttt ggttcttctt
    26521 cggaagccct ccggcacaca tgtaggcgtc cccaacagtt ttaatctttt caatatggta
    26581 cttttgtaca accgaatcaa actcgtaaac aaacctgtcg agctgatcga taagctcttc
    26641 ggaatccgtg tcgtcggtta tcttggtaaa accttgaata tccgagaata gcacggttac
    26701 catctgaaac ttttgcgctt taatctttcc aaattgctta agctcatcgg ctgtttcctg
    26761 aggtagtaca cgggccagaa gattttccga tttttccctc tccttcaaaa gctcattagt
    26821 acgttgaaat agtagggtct ccaagccatg catcgccttt gttcgcgagt agcttatcaa
    26881 gctatagagc aaaaaaaggg ttaataacac tagtattgaa ccaagccaaa tcattattca
    26941 gtaatatgca gaaagatttg ccatctaagc tacaatattt ttaaatgatt tgaaattaat
    27001 caggtaatat ttattcgtct cgttggcata ttcaggtatg taaaaggtta aaggatataa
    27061 gccattaaag gaaatctggt ttggactgat tttttctctt ctctctaact tataacacat
    27121 tgcccctcca gattcatggt tcagtaattt atgccgcaac cccggggttt aaaataacta
    27181 cgaatccgtt aaaagaaacc caagttttaa aggttttatt atttttgtat gaaaatttta
    27241 taaaactttg gaagtccggc taaagccttg aacattttaa aaatgtaagt atgaagggag
    27301 aaagtaaaac cattctggtc cctaccgact tttcacaggt ggcagagtat gccctacagc
    27361 atgccgttag ggtttccggc gtagtgaaag aacctatcac tattttgcat attgttaaag
    27421 cagaggccga taaagcagag gcagaggcca ggctggagaa aatagttcgg ggcacaaagg
    27481 agaagtacgg agttgacatc accccggtgg ttcgggtagg taacattttc accgacatag
    27541 ccctggtggc cgatgaggta caagccagct ttgttgtcat gggctcatcc acaattaaag
    27601 aaatggatga aaataaagtt tcatgggtgc tcaaggtgat taccagctgc aaggtgccct
    27661 tcattaccat tcaggagccc ccggtaaaca aacgctacga cgaggtggtt tttcctgttg
    27721 attttaccct tgaaaaccag gaaaagcaca agtggatatc ctacttcagc gatttttacc
    27781 tgtcgaggtt tcatctggtt aaacccaaca ctaccgatcc ggaactgctg gccaaaattg
    27841 acatcaacat ggcttcggca cgtagattcc ttgaccaaaa gggcgctaaa tctgttgaat
    27901 atactgttcc cgggttaaaa ccttacgagc aggagatttt gagccttgcc gccaacatta
    27961 gggccgatct gattgttctt atgactacac cccttgacag cgaagggcgg tttacggttg
    28021 atcccatgga gcagtacatt cttgccaatg ctggccacat cccggttatg tgcatcaatc
    28081 cacgctaggt atgattaaca ttccacaaaa actggttttt gccaccaaca acgtctataa
    28141 gctaaaagaa gttcaggcca ttcttggaaa tgctttccag ctgctatccc tacatgatat
    28201 tggatttatt gccgaaatac ccgaagacta tgagaccctg gaggaaaatg ccatgcaaaa
    28261 agcccgttac gtattttcaa gaacagggta cagctgcttt gccgacgaca caggacttga
    28321 agtagaggca ttgaatggcg aacctggcgt ttacagcgct cgatatgctg gagaagacaa
    28381 aaaatcaacc gaaaacatta aaaaacttct gatgaaactc aacggggttg agaaccgtaa
    28441 cgccagattt cgtactgtta tcgccctgac actctccgga aaggactacc tttttgaggg
    28501 aactgtcaac ggaaaaataa ttgactcccc caatggcttt gacggttttg gatacgatcc
    28561 ggttttcatc cccgatggct acaaccaaac ctttgcccaa atgcccctcg cgcttaaaaa
    28621 ctccataagc cataggggta tggctatatc caaactcgtt gattttttga aaaaacagta
    28681 aattctgtct taaactttac tgctaccgcc cttaaactaa ttaaagctga atggcaagag
    28741 taaccagtgc ggttttttta ttcctttttt tgctgcaggg agtcctgcaa gcacagattc
    28801 cggtaggtag ctggcgcgac cacttttcgt ttggtaacac aaaattgctg tgctacgaag
    28861 aagggaagat atactgcgga gctgataacg gaattttctc atacaatata gccaccggtg
    28921 aggttgataa agttacgagg gtgaatggcc tttcggaagt tggaatctcg gcacttagct
    28981 atttcccttc attgcaaata cttgccattg gctacgaaag tggaaacata gatttgctgg
    29041 ttcagaacag cgtagttaac cttcccttta taaaggaaaa acccctttcg ggcagtaaaa
    29101 ccattaaccg gtttaccctt tttaacaacc tgatttacac ttcaaccgat tttggactag
    29161 tggtggttga tccagctaaa agggaaataa aggaaaccta ctatatcgga aacaatggaa
    29221 ccggcatccg ggtgagcagc atggaggtat ttggggatag attctgggct gcaacttcca
    29281 atggcctgaa aagtgccctg tgctccgacc ctcttctggt aagctttgag cattggacta
    29341 ctgaaacatc gtttaccaac cctactgccg aatgcgctgg agttgctgct aaggatagct
    29401 acctttttgc tctggaaaga caggagaacg ccaacgacat tctctggcga ttcgatggaa
    29461 gctcatgggt tgaagttgac cggcctttgc ctgccaccct tcatcttagc gtaggcaata
    29521 ataggattct ggtaacctcc gctaatggga ttcaaaccta ccaatttccg gggattagag
    29581 atgaatttct ttcacccgca tcactgttta tcaacccaca agctgcaatt tcggtagatg
    29641 cagacaagct ggccatagcc gataagttta acggtcttat gtttggtagt tctcaggcgc
    29701 taaggtctat ctacccaaac ggtcctgcaa acaacaacca ccttgccata gatgttagca
    29761 gcaatcgtgt ggttgtggcc tcgggaggct acgatgcaac cttcaccaac ttgtggcatc
    29821 ccttcatggt tcatgatttc acgcatgagg aatggagcag ttttgctgat tatagctatc
    29881 atgatgccgt tgcggtaagg gttaatccag gcaacccatc agattactac gtagccagct
    29941 ggggtggcgg cctataccag ttccacgatg gcagcatggt tgcacacttt acgcctgaaa
    30001 acagctccct acagtccatt ttccccaatg ctccctactg ccgtataagc ggcctggatt
    30061 ttgacccaaa aggcaacctg tgggtggcaa acacaatggt cgcaaaccct atatctgtcc
    30121 gaaagacaga tggaacgtgg atttcatttc cctatgcatc agctatcaat tctgacaagc
    30181 tgtcaaccct cagctactcc aactacggaa cgctatggct actgctacct cgtggtcaag
    30241 gactttttgc cctgaaccct ggtaataata tcgacatcaa ggacgacgac cgctacagaa
    30301 aactacaacc ggccagtagc agcgggtcgc tattcaacgt tgatataaac gccctggcat
    30361 ttgataggga cgactaccta tggctgggaa caagccaggg tgttttagtg tgcttcaacc
    30421 ccggcaaggt ttttgatggg ggcatgtact ttcaaaagat taaaattccc gatgtggttg
    30481 aggggctggc agtttacctt cttgaaaccg aggaggtaac ctctgtaacg gttgacgggg
    30541 gtaaccgtaa atggtttgga acagcccgtt cgggagtatt cctgtttagc cccgacggaa
    30601 ccaaggagtt ggcccactac acaatccaga atagccctct cccgtccaat aggattaccg
    30661 atgtaaaagt tcatccggca tcaggagagg tttttattgc caccgataaa gggctggttt
    30721 cgtttcgaag caatgccaac caacccggat cgggctttgg aaaggtttat gctttcccaa
    30781 atcccgttcg acccgaatat gaaggtgaca ttaccattac cggtttaatg gagaataccc
    30841 tggtaaaaat taccgatata agcggaaacc tggtttacga gactttctct gttggaggta
    30901 tggctacatg gaatgggaaa aacagcaagg gacaacgggc ttcctccggg gtttacctgg
    30961 tcttttgctc ggctagtcag ggcgaaaaaa ctgccgttac aaaaattctt tttatcaggt
    31021 aacccatgct tcagaaatca aggggaattg tcctgcatgc ggtcaagttc cgtgataaca
    31081 gccttatagc tacatgctac agcaaggagc ttggtcgaac aaccattcag gtgaaccacg
    31141 catacggtag cgggaaaact gctggtctgg ctgtttactt ccaaccgctt gacattcttg
    31201 acttggtgtt ctacaaaaag gagacatccg aaatttcgct cttaaaagaa gtggcaattg
    31261 ccatacctct gagcgagctt cacttcgatc ctgttaaaat cagcatttca atgtttgtta
    31321 gcgaggtgat ttaccgcacc gttagggagg ttgaacccaa ctcagagctg tatgaatttt
    31381 tggaaaattc tataaggctg ctggatgtca tgcacgatgg agtggctaac ttccacctga
    31441 tttttctcat ccagctaacc agatacctgg gtttttaccc ggcaaacgga tggagcgaaa
    31501 ccatgccgtt tttcgactat aaaaatggct ttttctgtcc ttcaccacca tcccattctt
    31561 tcaacctcga caaaacttca agccgtcgcc tgggaatgct gctaggcacc ccattttata
    31621 ggtcaaatga aatatctcta aaccatgctc aaagaattca ggcaatcaac gacctgttgt
    31681 gctactacaa cttccacctt ggctcccctc tcgattttaa aacgcttcca attttaattc
    31741 aactttttca ataacgcact ttcaccacca aattggctca atttgtcaaa catttatggt
    31801 ggggtggcac aacttgagta tttttataaa acaatgataa atttttcttt tgaatgaaac
    31861 agattaacgc ggaatttgga gttgatacgg caaattcctt gcttaaactt attaatagcc
    31921 ataatgcgat agatctaact gcgggatgga caaggtttcc cataccagtg caggtggccg
    31981 atttggttgc cagggagttg aggcaaacgc atggacgtgc gcccatagag ggcacgctag
    32041 agctcagaaa gcatgtttct gagtacattc agaaaaaata ctttcggaca taccatcctg
    32101 cagctgaaat tgccattacc tcgggtgctg cccaggcaat tttttcggca gttgcggctt
    32161 tggtgagtga tggtgatgag gttatcattt tcgagcccac acgcaacgac acagcgctag
    32221 ccatcgaggt ttgtggtgcc cgacctgttt atcttaaact acacgaggat aacgcaagta
    32281 ttgattggca tgaagttcaa aagtcggtta ccccgcgtac caagctcata atcataagct
    32341 ctcctaacag ccttaccgga agcatccttt cgcctgacga catggagaat ttgcaaaagc
    32401 tgataaacgg aacaaagctt aaaattatca gcgatgaaac tttgagcgat atcgtgtacc
    32461 ctggcgttat tccatctagc gtggcgtttt ttcctaaatt ggccgataac gcatacataa
    32521 ttggctcgtt gagccaaacc ctgggaattt ccgattggga cttaggttac tgccttgctc
    32581 cagcagccat gatgaacaac tttcaacgta ttcagcgagc cattgccaac accgccaacc
    32641 acgccctaca gctggctatt gccgatttca tgagcaccaa cgaaacttct ttcccctacc
    32701 atgctgtaat tcagaaaaat cgccagattc tgcttgattc tctttcatct accaggttca
    32761 aaccactacc catgcaggcc ggatacactc aaaccctgga ctactcggac gtgtcggatt
    32821 taaaagatgt tgaatttgcc gaaatgctgg ttagggatta tggcgttggg gtttcgcctg
    32881 tttccattta cttccacgat aaaaccattc gaaagcactt gctgataaac ctggctgttg
    32941 aagttgacgt aatgaccgag gctgctcttc gtttatcaaa aacagcatag ggcttaacca
    33001 gctttactgc ccaattaaaa ttttaccagg atgggaatgc taccttcaat ccttgttttt
    33061 cagcaaaccc tttgtggata gaagtccaca tgcagcttga atatccagtc cccgagatgc
    33121 cctgatggtg gtgaatattc ctttagaggt aagggcatcg cgaaacttta tgatcgtttc
    33181 atcggatgcg ctgcgaaggg ccgattcagg aattgggtga aaccggatca ggttaaccct
    33241 acatcgtaag ttgtgcaaca gtcgggatat ttcccgaaca tgccgtggac tgtcgttaag
    33301 cccatcaaac atgatgtact cgaacgatac ccttcgttgc cccgcaatgt tggccttacg
    33361 gatctcctcc acaacgtcgg caatcgggta aacattctga ataggcatca gcatcctacg
    33421 ctcctcgtca aatggagaat gcaggctaat tgccaaatgg cagctacttc gggataggaa
    33481 ggtgttcatt gcagggataa ttccaatgga tgaaacggta atgcgtttcg ggctccagcc
    33541 cataccccat tcggaggtca gcacttcggt gctgcgaaga acattctcca ggttgtcgaa
    33601 tggttcgccc attcccatgt aaacaatatt cgtaacattt ttaaactcgg gtatgctccg
    33661 gagctggttg ataatctcac ctgaggaaag atttccctga aaaccctgcc tgccagtcat
    33721 gcagaacagg caacccattt tgcacccggc ctgtgacgat acgcaaagtg ttgcccttcc
    33781 ggtttcaggt atataggctg cctcgacaaa ttttccattt ccgctccgaa aaaggtattt
    33841 tttggttcca tcggccgaag ctgaaacgcc ttcgggcttg ctaaggccga cgtaatacct
    33901 accgacaagc tgttctctgg cgctcttgga aatgttggtc atcgcctcga tgctctccac
    33961 attctttttg taaagccagt cggcaagctg catagcggcg tacggaggca acccctcctg
    34021 gctaacaata tcctgtagct cggcaagcgt ctttccatat agcgggagta gttcgggcat
    34081 aggattaaga agttaatgga aacaatcgag gtagtatgat tttctggtaa attgtaaatt
    34141 aatgaattgt gaaaagctac caataggttt aaaagtaaat ttcagcgttt attctatcgg
    34201 gtggtacacc ccttgaaatt agtaagctcc ttgtttcaac aaccatttcg gtgctgccac
    34261 aaaggtagta atttcggttt ggatctagct catcccattg cagcaggtag ctggtaacac
    34321 gaccaacata atggcttccg ggcatacacc tcgagcagca ctggatatag tttcttccta
    34381 gcttctcgga aagataatcg gaaaagtaaa agtactcggg atatggagca ccatgtatta
    34441 aagttgcccc tctaacctta ttggatagga gtgcagaaat gaaaggagca accccagtac
    34501 cggcggcaac aaacaccggt tcggatttaa agctggtaaa tgctccaaat ggctttgaca
    34561 ccagaatcgg gttacctgga ttgagcattg acagcatagg agttagcttc ccatcgggtt
    34621 tttccgtata gagaaccgat atcaactcgt cttcctcgga agaagcaatg ctgtagaacc
    34681 ttggttcaat atcggtagaa actccaaggg caacaatctg ccctgcacta aaatcgaact
    34741 gttttctgaa tttcagaaga aacgctcccg gggctagtcg ggtattcgaa atcacctgct
    34801 gtaatctata atcgccttcc ttccatgctt tcatgaagta atcggaaata attttagtac
    34861 tacgatttat cagctaacat aagcctagta gggggtaaat taccttaatg ccttaacagt
    34921 gtgcagcaat tattcctgaa gcacgggttg cttaaccctg gcaagcaagc acgttagact
    34981 aaaacatagg tttaccacaa cgaaagcagt aacattcaaa actacaaata gtttacagta
    35041 aatttttgaa aacacacact cctgcgttca ggtgagaaaa ttataactta aggctgtttg
    35101 acttcttgct gtgtattgtg ttggccaaat cggtaaaggt agctcaggct aaaattttgt
    35161 atgatagccc ttgactcgct tggcatgccg gaatacgagt aatcgtaaag atcacccaac
    35221 ccgtaaccaa acctggcttc agcctgaatg gttccccatt ggaagtcatg gcttaggcct
    35281 acaccaccca tcaacccgta gtcgaaacgg ttttcgcgaa gaatgttgat gtccaccttg
    35341 ttcagcctgt actttccggt ggagttatcg ccctctttgg cgctgaggag gtaggcaaaa
    35401 tacggaccgg cattcacatg cagcattact atccccaact tcaacctgaa ctgaataaac
    35461 atgggcaact ccagatagag gttaaccctt ttaaaaagtg tatcacccaa ctcctcaaca
    35521 tcaatagctt ttaaataacc cctgctggtc aggtagagct cggattgaaa ccctacatac
    35581 tttgcattga agtacttgaa ttcaagtccg tagtccagcc cgctaccagt taaaagttta
    35641 gtttcagcat caggcttaaa agtcatggtt gaagtggtat aagcagccct tagcccaaca
    35701 tatggctgag ccttaatgcc ttccgaaaca agcaaaatga agaagagaaa aattattacc
    35761 cttcccttaa aataattggc aatgtcagcc ttattcattt caaagttaca cattggcatg
    35821 caagtatatt atcatgaaag cttccactca attcttcgca cataatgaaa ctatcagcgg
    35881 taatgaagct attatggctg aggcttgttg gatatgtaga agaattgcct aagccagctt
    35941 gtcttcgacc gatttcacct ttaagctaat ccttccggag cctcccttgc gaatgtctaa
    36001 gcttactgta acgtaaaccc tgtcgcaatc gggttcgagc aattggtaag ctttttccac
    36061 cacctgcaag gcctcctcaa gggagctggt ctccacaatt gttcccattg cggtaagctg
    36121 tgaggcatgg ttcaacgaac gaacgccctc caccaccctg gccacgtagg gactaacact
    36181 ggcaccctta tcggtgggaa acatggcaaa attcatcagt acagacatag gtattttatg
    36241 gtttttgcag gctaaccgaa atctcgttat cccagttggc ccgaacgttt atcacaccct
    36301 tatttttccc ttcagtaaac gggattaccc tttcgggctg aatgcgcttc aagtagttgc
    36361 cgctatccca ggtaccgttt gcgtttaaat catcaataaa tcgaagttta tactttgcag
    36421 gggctatgaa cgtaaaggtc acggattggt tgcctacaac ggctttccta tctaccactg
    36481 catccttttc ggtcagcagc tcagctacaa ccgccttact tacaccagaa aatttagctg
    36541 tgagggttcc aaaactttcg gggtcagctc cggtaagctg aagcttaagg gtgtcgttca
    36601 gggttgaggt gtagctttca aaagcacccg gcaaaacaat cattttgtag gttacattgg
    36661 gtttccagga ggtgttgaaa cggtaaatcc tggggtttat cgagtctgcg acaagcttca
    36721 cgccaggctc caaaatgctg tcggtttggt taaaaatctg cactagcgca ccattcacct
    36781 ttcttgcagg tgaaggaagg gtaaactcaa agggaactcc ggggatagca actgccatgt
    36841 tcttgatgga ggtttcaacc ttgaatccgg ttggttttac aacttcctcc ttctttccgc
    36901 gctttgaacg ggagtcattc ccggcttcct cggattcata gtaaacaaac ctaagggtat
    36961 ctatccgagg ctgaagcatg ttgagggaat cggttttgag gtacgacaaa gccagcttta
    37021 gcgtgtcagc agccgaaatt ttctccgaga taatccaaag tttaacggtg tctccctcgt
    37081 tgtcgggttc gggaatatac catttctcgc taacggggtt gtcaacttgt accagcttaa
    37141 aaccgccaac cggtttgcgg gagaagttta gctcaattag atcccgctga gggcggttgg
    37201 tacccgtaag tagctggtag ggcagtggct cgctaaatgt tcgcagcctt acccacctag
    37261 acgaatcgga agcgttcatt agcatatcgt gtgttacctt atcctcgaca aatgcaatat
    37321 cctcaatacc ctggttgtaa aggtagttac cgttggcatc ggtaatggca acaagcttgt
    37381 aatcaatgtt tttaagattg tttaccttga acctgccatt tttacccgac tttgccatat
    37441 gtgttggacg tttaatatag ggtagggaat cggcaaaact actgtagagc atgattagcg
    37501 ctccctcaac tggttcatta gtaaaagagt tgacaatagt acctgccaga gaagcagtgt
    37561 caatggttgg gccggttgaa aatgcaaact caaaatttgg aatggcatta ttctcattgt
    37621 tatcccttat ggcatcgcca aagtaaaccg tgtaggtggt gttctccagc agggtatctt
    37681 ttatctccag ctcaattcgc ttgccctttg gcttaatttg aggtctgtta ttcaggggtg
    37741 gtgaaaccag caacttttgc tgcacgtcct tcaatgccag gaactcatca aactctaata
    37801 ccagtttttc ccctctgaag tttatggcgt taggcttcgg gatgctgttt accagcgctg
    37861 gaggaatggt gtcctttggg cctcccgttg gggttaccac cttggcacag ttagggaaaa
    37921 gtgaaagcag agcgacagca aaaatggtta tcagaagaat tttttttagc atctatattt
    37981 atcctttgtt taagttagca aagttaacct aatcgcaatt atctttgcag cagctagcca
    38041 aaaaaccttg aaaattagct gtgtgtggct gttgctggat ttaaaacttt acacctgctt
    38101 attccctaca taatggataa caagaacttc gaggataatt tgtttttcta ctggactaaa
    38161 aatcttgtag gcacaaattc ccggtttatc ctcaacctgg ctattgctat tcttttcggg
    38221 acgctttact cctttaaaat cgctcaaacc aacgtaattc tactcatatt tggggtggta
    38281 tcgcctgtaa tttttacact atgcctctat aaccttattt tgcttgttag cggggataaa
    38341 caggaggtgc tgaattttcc atcagttttt ttggtgaaaa aaagcaaccg gcttctttcc
    38401 attttcgaca gctctctggt ggttttgctt gggtggctaa tttaccgggg caccctaaac
    38461 tacttctttt tccgttttct acttacattc tttattccgg tgttgcttat aattttcctc
    38521 aggggtttgt atttcttcat aactggctaa aacagggtta aaggtaagcg aatacttgtc
    38581 cgcctactgg tggattccag aacttgtccg cctactggtg gattccagat tccagaactt
    38641 gtccgcctaa cggcggattc cagaacttgt ccgcctgctg gcggattcac ccttcgacag
    38701 gtgttacagt gagtagtt
//
LOCUS       DAOPHX010000007        33306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_1241_length_33306_cov_6.649183, whole genome shotgun sequence.
ACCESSION   DAOPHX010000007 DAOPHX010000000
VERSION     DAOPHX010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 33306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33306
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1241_length_33306_cov_6.649183"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PK502_01230"
     CDS             <1..478
                     /locus_tag="PK502_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPG99470.1"
                     /translation="FVGQNIGANKIDRVKRGLRSTLKISISFCVVNIAIILIFGHSLM
                     GIFTKSSDVIAIGYQYLLVVSLFYIIFAVMFSINGLLRGVGAAVVPMYITLLSLWIFR
                     IPLAYLLSKTFGMGALGIWWSIPIGWTMGAIAAVAYYRHGSWKNKSLVKVRQQAAD"
     gene            871..4029
                     /locus_tag="PK502_01235"
     CDS             871..4029
                     /locus_tag="PK502_01235"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPG99471.1"
                     /translation="MLKDYLSRIQDTINKGDAREESYYSHLDSLIKEFAIENGIKKID
                     VTILPKKTEAGNPDFRVWDGKNHITGYIEAKDPSVDNLDRIEESEQLTRYRSTFPNLI
                     LTNFYEFRLYRDGELVRKVTIGRQRIAQKLKTAPPVENEKEFNDLLGTFFSFSLPQVR
                     SAKALAQELAKRTRFLRDEVVSIELAEEGRIGKRDIMGFYEAFRKYLIGTLTEQSFAD
                     LYSQTITYGLFAARTRSDNTFNRELAFRYIPNTIGILRDVFRFISLEDPPKPLQMIVD
                     DIAEVLSVTDVKKILDDYYQQGKGQDPVVHFYETFLSAYDPSIREKRGVYYTPEPVVG
                     YIVRSVNQVLKTHFNITDGLAGEDVTLLDPAAGTLTFPAEAVKLAVEEFTRSYGSGGK
                     TNFIRNHILKNFFAFELMMAPYAIGHIKMSVLLEELGYRMGDDERFQLYLTNTLEMDE
                     LEQISIPGLSSLSEESHLAARVKKEQPILVILGNPPYSGISGNINQWTEKLLKQNLDG
                     AQSYYEVDGKPLGEKNPKWLQDDYVKFLRFAQWKVQKAGLGVVGMITNHSYLDNPTFR
                     GMRQSLMGTFNEIFILNLHGNSHKRETSPDGSKDENVFDIRQGTAIAIFVKQKGKNGC
                     RVYHKDLFGLREVKYGWLGSNEIKVDDYRVLKPKSPWYFFIPKDTDSIQDYLAWKKVN
                     EIFPVSVTGIVTARDNLAIDFNLDALKNKIMMFRNLKIDDNTLKQAFNLTDNYQWKLE
                     EQRKKIQKVTDWQENFTKILYRPFDTRNIYYQENIVFRTRREVMRHMLKDNIAINTVR
                     QVKTGKSWQHCLVSDRITESSYLSNKTSEIGYVFPLYLYKEVASKKSHFQSMLVFEPE
                     VEYGGNRTPNIDKKILEKLNKSYRKNPTPEEILYYIYAVFYSNVYRRKYAEFLRIDFP
                     RVPFTKDYDVFARMAELGNQLADLHLLKGKLLENPISKYQGESENDRIEGIAYDEQKG
                     RIYINKNKYFDGITPEVWGYEIGGYQVLQKYLKDRKGRNMDDSKHYSRVITAISATIE
                     LQRQIDETYPLVENELVEFLSA"
     gene            4171..4464
                     /locus_tag="PK502_01240"
     CDS             4171..4464
                     /locus_tag="PK502_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99472.1"
                     /translation="MASRKDLKKDVDSLVGEVVNDCFTYMTLHGDKNRDKAEGIISKI
                     MESRNTAIKKINNPGKLTDKKATKAHFKALQQELLVSADECFTELSKLPKDLG"
     gene            4647..5714
                     /locus_tag="PK502_01245"
     CDS             4647..5714
                     /locus_tag="PK502_01245"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPG99473.1"
                     /translation="MKPLNRIFIFLLLLLSVAKPSSAGRVVPNSINRSSIREIEASIR
                     NGDYGKVTSIVVLNRQGAILYEKYFGFSTRNSLHTISSVTKSITSLMVGICLDKGFIS
                     SIDSPIYSYFPEYKEVFEKDSTKKLITLRHLLQQTAGLNFKEWQFPYNYASNSLMATI
                     EENRSWVERFFYLPVDTLPGVKFSYNSLASQVIAEVLSRASGIAYDEMVRKFLFKPLS
                     IENFLWESYAGNSLPAWGGLSLTTFDMAKIGLLVINGGIYGGRRLVSSEWVESSTVGR
                     VRVNDNIYYGLHWWVDILPSGKPLPFAAGYGDQYVFTAPDRGLVVALNAQNFSDFRFS
                     KSAIDLANILLSSVEVENFQN"
     gene            5726..6736
                     /locus_tag="PK502_01250"
     CDS             5726..6736
                     /locus_tag="PK502_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HPG99474.1"
                     /translation="MSDLKTSYMGIELKNPLIAGASSLSLNIESLKAIEAAGAGAVVF
                     KSLFEEQIQLEALELENELEEYNERHAEMVKLFPTLKHAGPADHLAKLRKAKDTLSIP
                     VIASLNAMYPDTWVEYAKEIEQTGVDGIELNFYTTPKNFEVEGKTIIQSQVDTLKSVL
                     EKVTIPVSVKLSPFYANPLDVIRRFDETGIQGFVLFNRFFEPDIDIEKEELVQTITLS
                     NEDDLRLPLRFTGLLYGNIVADICSSTGIMTGADAVKMLLAGATTFQVTSTIYANGIE
                     QITKILGEIEAWMHRKGYKSLHDFRGKLAKKNLHDPFAYLRAQYVDILLRSNDIIKDS
                     AI"
     gene            complement(6935..7207)
                     /locus_tag="PK502_01255"
     CDS             complement(6935..7207)
                     /locus_tag="PK502_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPG99475.1"
                     /translation="MTAKTKAISVKGRVQGVGFRYFVLRNAKRLGIRGFVKNMPDRSV
                     YIEAQGQEENLNELIGLCWKGPERSRVDSVETNEVVRKEWNDFYVE"
     gene            7321..7809
                     /locus_tag="PK502_01260"
     CDS             7321..7809
                     /locus_tag="PK502_01260"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HPG99476.1"
                     /translation="MVVDYVLIVLAAALVVVGLVGCFLPVIPGPPLSWVGLLLAYFTN
                     FVNFDWSLLLWTGIATLVVVILDYVMPVWVTRKMGGTKRGVWGSAIGLVVGLFVFPPL
                     GIIVGPFVGAFIGELTARDKSNNPLLSATGSFIGFLVGTGLKFAVSGYIAYRFTVEVF
                     FR"
     gene            complement(7953..8228)
                     /locus_tag="PK502_01265"
     CDS             complement(7953..8228)
                     /locus_tag="PK502_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPG99477.1"
                     /translation="MMIYVGNIAYSMTPDDLKELFAPFGNVLSVKIIADKTSGRSKGY
                     GFVEMEKDEDGQKAISALNDTQVKGRNIKVNNAFRKDDSKTEGKQKD"
     gene            complement(8338..9339)
                     /gene="argF"
                     /locus_tag="PK502_01270"
     CDS             complement(8338..9339)
                     /gene="argF"
                     /locus_tag="PK502_01270"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPG99478.1"
                     /translation="MSFNLRNRSFLKLLDFTPKEIQFLLDLSADLKKAKYAGTEQQQL
                     KGKNIVILFEKDSTRTRCAFEVAAYDQGAHVTYLGPSGSQMGKKESMKDTARVLGRMY
                     DGIEYRGYSQEIVETLAKYAGVPVWNGLTTEYHPTQILADFLTMREHSSKPLSKVTFC
                     YMGDARNNMGNSLMVGAAKMGMDFRACAPKACWPKEELVKTCMEIAKTTGARITLTEK
                     VEEGVKGCDFLYTDVWVSMGEADSVWDERIKLLKPYQVNRKAMELTGNPNCKFMHCLP
                     SFHNRETAVGEDIFKKFGLESMEVTDEVFESDASIVFDEAENRVHTIKAVMVATLGS"
     gene            complement(9501..10460)
                     /gene="arcC"
                     /locus_tag="PK502_01275"
     CDS             complement(9501..10460)
                     /gene="arcC"
                     /locus_tag="PK502_01275"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPG99479.1"
                     /translation="MSKLAVVALGGNALLRGNQVGTIEEQEQNTTETLENLVYLIKEG
                     YNLVISHGNGPQVGNILMRNDAGEQLYGIPQMPLDVCVADSQGGIGYMIERMLRNVLK
                     NHGIDKEVCCLVTPVIVDKNDPAFSNPTKRVGRIYDKAQADALASQKGWIFKEEVKDT
                     GSGWRRVVPSPKPKGILNIKVIDQLVNQGIIVIASGGGGVPVYIDEEKNVRPAEAVID
                     KDSASALMAAEIKADEFYILTDVPFVFLNYKKPNEKKLEFLNHADATRYLQEGMFGEG
                     NMAPKIRACLNFIENGGKMSVITEATKLEDMSYGTKITMAYED"
     gene            10782..11474
                     /locus_tag="PK502_01280"
     CDS             10782..11474
                     /locus_tag="PK502_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99480.1"
                     /translation="MRKPIAGLVILSAFILCSCGGRVKNNSISEGVITYDITYDSAIQ
                     SRYDAWLLPSELIVRFNSNHSINSIEALGGAISISIIKNPERNEFSTLLKIFNKKLYH
                     SEPIEQERYPALYSRMPAVKLISEGETCEFLGYSCTKAKGYFADNPDFPFEIIYTSQI
                     QIENPNAHTPFEEVKGTLLQFNIRLNNLTMHATARSVKSEHVPLELFAPPSDYVVTDS
                     QTIANLIYTLQQ"
     gene            11704..14166
                     /locus_tag="PK502_01285"
     CDS             11704..14166
                     /locus_tag="PK502_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPG99481.1"
                     /translation="MASDSNRPNVKEKNNATKEGQDNGKNKLLRDERVRFTIGLFLLL
                     LGVYFLISLISYLFTWKVDQSLEWQSLFSKAEYEVKNWTGRFGAVISAQFMNHWFGLS
                     SLALPSIIIVFGLKLLKLYRKNIWRFIFNIFIGTLLCSLVLGFLFETAKGFLGSGLGG
                     AQGLFVSKWLSALLGKVGTSFLLLLLVIAYTIFIKPSTLHWFRRTIRGLLIRMRRPVS
                     RKNDKRQNKKEKTAEELAGQVDSEVLPNNTDAAVEVTLEKFIPEPNSELVDAEPFELI
                     EEETAGTNSSNAENTPEKSDVEFTIEKINEVELNPVEVNGQELYDPTLELSRYQRPLL
                     ELLEDYKNTTSPVSNETLIENKNRIVETLSHYKIQIDKIKATIGPTVTLYEIVPAPGI
                     RISKIKSLEDDIALSLAALGIRIIAPIPGKGTIGIEVPNQNPEIVSMHSIIKSQKFQE
                     CKYELAIALGKTISNEIFMLDLAKLPHLLVAGATGQGKSVGLNAIITSLLYKKHPAEI
                     KFVLVDPKKVELTLYSKLERHFLAELPGSEEPIITDTQKVISTLNSLCIEMDNRYELL
                     KLAQVRNIKEYNEKFVERRLNPNKGHRFLPYIILIIDEFADLIMTAGREVETPLARLA
                     QLARAIGIHLIIATQRPSTNIITGVIKANFPARIAFRVPSMIDSRTILDHPGANNLVG
                     RGDMLVSAGSDMVRVQCAFIDIPEIERITDFIGNQQGYPEAYQLPDYAPEGQDGFREI
                     DLNKRDELFEEAARMVVQTQIGSTSLIQRKLSIGYNRAGRIVDQLEAAGIVGPFEGSK
                     ARQVLIADELSLEQILRGLSNQ"
     gene            14229..14873
                     /locus_tag="PK502_01290"
     CDS             14229..14873
                     /locus_tag="PK502_01290"
                     /inference="COORDINATES: protein motif:HMM:NF027898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPG99482.1"
                     /translation="MRSLMLFVFALLSVNLVAQESQDVPQTFWDFSSKMLEASSINAT
                     FSLTIENRQENVANTQQGEILIKGNKYLLKIMGMEVYFDGSTKWQYIGEANEVTISTS
                     GGELDSPLDDPAGIFRDYESRFKVRFISETTVKNRTLVEMDFYPKALDSPYVSVKLRF
                     DKVSLEPVFIRYHGKDGTSYIVNISKFKTNVPAPDGTFTFDISTRKGIEVIDLR"
     gene            14923..15465
                     /gene="cysC"
                     /locus_tag="PK502_01295"
     CDS             14923..15465
                     /gene="cysC"
                     /locus_tag="PK502_01295"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HPG99483.1"
                     /translation="MTSCTPATIWLTGLPSSGKSTIGLGLGRMLDGIGVGYVLLDGDE
                     VRSGVCSDLGFSDDDRKENIRRVAHIAKLLNSQGLVAICCLVSPLQSMRDLARSIVGS
                     RFFYEVFVNASLETCMKRDVKGLYAKAEQGQLNNLTGFSAAYENPTDPNLVIFNNSIG
                     VEEAVNMLFTHVVDWLEIPW"
     gene            15580..16485
                     /gene="cysD"
                     /locus_tag="PK502_01300"
     CDS             15580..16485
                     /gene="cysD"
                     /locus_tag="PK502_01300"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HPG99484.1"
                     /translation="MSKFKLNHLRELESESIYVIREVVSQFENPVMLFSGGKDSIVMF
                     HLARKAFYPAKVPFPLLHIDTGHNFPETLEFRDWLVKETGVQLIVRYVQDSIDRGRVA
                     EEKGYNASRNKLQTVTLLDAIEELKVDCAMGGGRRDEEKARAKERFFSHRDEFGQWDP
                     KNQRPELWNLFNGRKNLGEHFRVFPISNWTEMDVWQYVLMEQIALPSLYFSHERDVFM
                     RDGVLYAKTPFLPMKDDEVAEKRIVRFRTIGDATCTGAVESAASTLEEIIREVAATRT
                     TERGGRADDKRSEAAMEDRKIEGYF"
     gene            16598..17875
                     /locus_tag="PK502_01305"
     CDS             16598..17875
                     /locus_tag="PK502_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPG99485.1"
                     /translation="MEQEITSSAYLDMELLRFTTAGSVDDGKSTLIGRLLYDSKAIFQ
                     DQLEAIERASIKKGEDSLDLALLTDGLRAEREQGITIDVAYRYFHTPKRKFIIADTPG
                     HTQYTRNMVTGASTANAAVILVDARHGIVEQTIRHAYIASLLRIPHVIVCVNKMDMVD
                     YAQESFEAIQRRFLEFSSFLDLYDLRFIPISALLGDNVVERSKHMDWYQGPTLMYLLE
                     NLYISNDYNQLDARFPVQYVIRPQSAEFPDYRGYAGRMASGTFRVGDRVKVLPSGFSS
                     VIKGIDFWTDSLDSITAPQSGTILLDDDLDISRGGLIIPEENGIKQGQEVDLMICWFS
                     EKPLVVGGKYAVKHTTNDLRCLIKEVKYKVNINTLEKDYHEKSIGMNDIACITIKTTK
                     PLFYDSYRQNRITGSLIIIDEATNVTVGAGMIV"
     gene            complement(17876..18313)
                     /locus_tag="PK502_01310"
     CDS             complement(17876..18313)
                     /locus_tag="PK502_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPG99486.1"
                     /translation="MYCYEYPRMLVTVDAVIFRTDTQGHPVDLLLIERKNEPYKGCYA
                     LPGGFPEMNELLSDAAARELKEETGLDGITLKQVGAFDRVDRDPRDRNISVAYLGFAS
                     ADSPLPNAGDDAATASWFPLNNLPPLAFDHAEILEKALSNIGI"
     gene            complement(18495..19268)
                     /locus_tag="PK502_01315"
     CDS             complement(18495..19268)
                     /locus_tag="PK502_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPG99487.1"
                     /translation="MEKGVVSKSMAALRREPTHTSEMVNQLLFGETFDMVERQGGWLK
                     VKGHTDGYSGWMDERLSLQLPDNSPIFTDKPKIASRLFRAKAASGFTTYLCPGSQLHS
                     FDGKTFRNGSSSFVCMDTPFAKLPDNPRKKILALSTTFMDAPYLWGGRSLFGVDCSGL
                     VQVVFEIAGTMLPRDARDQAGIGQTIEFANEAKPGDLAFFDDENGVITHVGIVAEDAT
                     IIHCSGFVRVDRFDHQGIFNLEAHRYTHKLRIIRRVIFD"
     gene            19502..22846
                     /gene="mfd"
                     /locus_tag="PK502_01320"
     CDS             19502..22846
                     /gene="mfd"
                     /locus_tag="PK502_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPG99488.1"
                     /translation="MQHQHELYKLYLKHPSLKQLLDAGQNLEGGFFLKGLAGSAVAFV
                     LSGYVEQRRRPSLVVVNDKEEAAYLFSDLSCLLPENQVYFFPSSYKRSVQYGQTLPDA
                     IVQRTDVVALLPALNENDERPVVVVTYPDALFEKVVSLDTLSRNSIKIQKGEKVSPDF
                     ICDTLTEYGFQRSDFVYEPGQFSVRGSIVDVFSYSASTPYRLDFFGDEVETIRSFDVE
                     DQLSKELLSEVVIIPNLQSPEDNPSRILLTDLLPPSTTLWVKNPELVLNRMDELSRIV
                     STATQLNASNFASRDIFSNSLLTRSTLYFGIKPLSVQLPTFDFGTSPQPAFGKNFELL
                     VQKINEDAGRGYQTFILSDNSAQLERLDGIMHGIAPDIAYNPVEGTISEGFVDHNLRI
                     NLYTDHQIFERYHRYKLKHEFNRRDAITMQELVDLQPGDYVVHIDHGIGIFGGLVSTE
                     INGKKQEAIRLVYQDNDTLLVSIHNLHKISKYRGKDAESPKIYKLGSGAWQRLKQNTK
                     RKIKDIARELIALYSQRRSQPGFSFSPDTYMQQELEASFVFEDTPDQLSATKAVKEDM
                     EAERPMDRLICGDVGFGKTEVAIRAAFKAVADSKQVAVLVPTTILALQHYQTFTSRLK
                     GFPCTVEFLNRMKTPAQQREIRQRASEGKIDILIGTHALLNSTLKFKDLGLLVVDEEQ
                     KFGVAAKEKLKQLKLNVDTLTLTATPIPRTLQFSLMGARDLSIINTPPANRHPILTEV
                     HTFSTAIIKEAIDYEMDRGGQVFFVHNRIQNIAEVELMVNKICPKAKTAVAHGQMEPS
                     KLEKIMLGFISGDYDVLISTSIIESGLDISNANTMIINNAQMFGLSDLHQLRGRVGRS
                     NKKAFCYLLAPPLETLTADARRRLRAIEEFSELGSGFSIAMQDLDIRGAGNILGAEQS
                     GFITEIGFEAYQRILDEALEELRESEFVTIQTQAEHETQREWKPSYADCHVETDMEIL
                     LPDSYITNTTERIKLYREIDSLHNAEELERFRNEIRDRFGPLPRQVEDLLSVVRLRWI
                     AVELGFEKLILGNGQLMAHFISNQLSPYYRSSTFEGIIRFIQQHPERFKLKEGKDKLT
                     IRIAGVGEISEAIETLQKLHETLPVK"
     gene            22887..23609
                     /locus_tag="PK502_01325"
     CDS             22887..23609
                     /locus_tag="PK502_01325"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPG99489.1"
                     /translation="MNLIIDVGNTQVKVALMEQGEIVFLKSFSTLSAGSMEEILSTYL
                     PHKAIVSVVGNFQPEILSYLSQKLMVHVLGSKSKLPLKNTYETPLTLGYDRIAVAVGA
                     ITLYPNQNVLVVDCGTAITYDVVTSDEIYLGGGISPGISLRFKSLNDYTAKLPLLSIS
                     NTFSLIGRNTNDCILSGVLNGVVAEVDAYIEKFNLIYSNSRVVLTGGDSSFFVDKLKN
                     SIFVNPNLIYTGLNRILELNAE"
     gene            23599..24897
                     /locus_tag="PK502_01330"
     CDS             23599..24897
                     /locus_tag="PK502_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99490.1"
                     /translation="MQNSRKILLLVGFTLAALEVFSQNIYTYSPYSRFGIGIIPTHGF
                     SQTRAMGGVSQSVRSPYMINYLNPASYAAQDSMSFTFDFGLESNNSSFSGYDQYLNKV
                     SASSSAGGIHHIAIAFPVSKKIGMAAGIAPYSSVGYRLIRFETDPYILSTIGRIKYEH
                     AGKGGINQLFVGAGYAPFKNFSIGANFIYYFGSLDYNNNIIFPSSSYSYTNASVGSSI
                     MVSDVSFNVGFQYRLFLDEDKNNSLGFGVTVDAGSDINMKYKWLTKLEGSTYVDSLIP
                     HPQSKSKFKLPTGVNSGISYTFKDKLFATAEYYYQDWTKTEAFNTTLQMGKMEAFRLG
                     VEFTPNRSDLKSYLKKISYRAGGFYNKTNLIINSRQIEEYGISLGFGLPFRNNTSFNL
                     SAELGRRGTNENYLIKESFFVLNLGITFHDSPWFFKRKYY"
     gene            24916..25482
                     /gene="lptC"
                     /locus_tag="PK502_01335"
     CDS             24916..25482
                     /gene="lptC"
                     /locus_tag="PK502_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPG99491.1"
                     /translation="MRITLFKGYKYILPGIAIVGTTFFFGCKTDTEAIQYFSDKKSIP
                     GVTAYNSELEYTENGVVKIMVKAPITIYYQFSEEPYTEFPEGITVYTYDDTLGVESNL
                     TAKYAIYYEKKMLWHAKNNVVARNRKGEVLNTEELYWDQGKHLIYTDVNVKINSVNGN
                     IYGKGLVSDEKFDSWEVKEPYDGTILLE"
     gene            26018..27289
                     /locus_tag="PK502_01340"
     CDS             26018..27289
                     /locus_tag="PK502_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPG99492.1"
                     /translation="MGTTIIIILISLFFSFFFAGMEIAYLSANKLKLELDKKKGGFTS
                     HVIAVLAGNKEQYIATMLVGNNIALVIYGIEIAVLLTPALGKLIQIPSLLLLVQTLIS
                     TIIVLVTAEFLPKSIFRLHPNAFLRFFALPVYILYIIFYPISKFTSWLSYWIIRLVFR
                     IKVKHDGSKPIFNKVDLDFLVDQASSADDGEGDHTQELKIFQNALDFSEVRLRDCMIP
                     RTDIEAIDVNATVDELRAKFAETNYSRLPVYSNSIDNIVGYVNSKVLFINPQSIKSKL
                     INIDFVPETMPANKMLTRFIKEHKSIAIVVDEFGGTAGLVTIEDIMEEIFGEIDDEHD
                     RNEFVEKKLSDNEYIFSGRLEVKHLNENYQLDIPESDDYETLAGFILFKHQNIPKLNE
                     TIEFEGFIIKILRVNETRIELVRLTRIKGKG"
     gene            complement(27484..27978)
                     /locus_tag="PK502_01345"
     CDS             complement(27484..27978)
                     /locus_tag="PK502_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPG99493.1"
                     /translation="MKRIGILSDTHGTLDDGVMEFFSDVDEIWHAGDIGSLSLADRLA
                     SFRPFRAVYGNVDDARVRLSYPLSQHFRVEDIVVFMTHIGGRPGRYEPIVYEAINSNE
                     VNLLVCGHSHILLVKFDSKHNLLFINPGAAGNNGFHKIKTVVKLDIEGKQMKNLQVWE
                     KTRF"
     gene            28403..31522
                     /locus_tag="PK502_01350"
     CDS             28403..31522
                     /locus_tag="PK502_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPG99494.1"
                     /translation="MRKLSGFLSFFFIILLVLPASKIAAQNIKISGTVISLTGGYPIR
                     GVTVVVVGSARGTFTDSLGRYELVTPSISKTLRFTCIGYKTQEVDIAGRRVIDVALEE
                     EAIEMQEVKVTALGIMRAERSTGYALQSVKGEDINRTSETSLLNALQGKVAGAIITST
                     SGAVGASSRIVLRGINSLNTDNQPLFVVDNVVLSNANFGNTTTEGVNRGSGVADINPN
                     DIEDITVLKGPNAAALYGSRAANGVIIIKTKSADVDDHFGVSFNSTITMESPMRLPEF
                     QNKYGQGSGGLFEFYDGMGNGINDNVDESWGPPLDIGLMIPQWNSPVAGGIRQPTPWI
                     SHPDNVKNFFETGMTFTNNVSVEGADESYAARFSYTNTEQKGMVPNTDQSKNNVMLNA
                     SISPYTYLTLNASLGYVNTHSDNLPAYGYDAQNVMQQFLWFGRQVDVADLRQYRFPDG
                     SKRNWNYSYHNNPYFTLYENLNGLDRDRLFGQGSINLKFTDWLNLKVGAGIDYFKNYN
                     NARSAFGDIDSPLGYYGESHLTFRELNTDFLLSFNRNLSSKLSLSFNLGGNLMRQYSQ
                     SLNSYATELSVEDVYTLENSRGPVVSINSLSRKQINSLFYNGQLVWKNALFFDFTGRN
                     DWSSTLPLNNNTYFYPSFNLSAVLSDLFDFQSPTLNMVQVRAGWAQVGTDTDPYQLQP
                     VYRFDQGWHESTKLPAIYIPNDLPNPNLKPQRTNSLEVGATLKLFMSRLGIDLTYYNQ
                     KTFDQIIRLPVSATTGYTSKIVNAGEIDNSGIELTLDGVPVRTRSGFEWSINLNFAWN
                     KNEVKELAEGLKQYVLGGYWSLDVIAIPGQPVGTLYGYDFKRNENGKIVFYDGLPAQG
                     EIHALGNYTPDWTGSIANSLRYKNLSLNFLIDARFGGEIYSMTSTWGRYAGVLKETLK
                     GREGGIIGVGVMQNEAGEWVPNNVVVTAEEFNKAAYNNNIAYSSVFDASFVKLREISL
                     GYTFNHIANNLIKEISIAVVGRNLALLYARVPHIDPETAFSNSNVQGLEFGQLPSPRS
                     YGLSLNLKF"
     gene            31535..33094
                     /locus_tag="PK502_01355"
     CDS             31535..33094
                     /locus_tag="PK502_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HPG99495.1"
                     /translation="MDTPMWRWFKIYRWIAITGILVGMVSCTGDFEELNRNPNAIGAN
                     ADHPDMLLTHAVESLGDQLFDVWLGHEIGSCWVQHMAKVQYTDEDRYVPRNDVINSVW
                     NNLYAGPGIEIQRIYEIGQRKGHANYIGVALVLKSYLFSVLTDLYGDIPYTQAFNPDS
                     TLTPQYDTQESIYLNLIQNLKYANELLNPYGDGIEGDILFHGDILKWKKFANSLRLRI
                     LLRMSGHAEQEQFVKSEMSSILGNSTDNPIFTSNSDNAALHYLGSYPNNHPLNETYKT
                     RDDHRVSRTLVDVMYNPGNHDNPDWRIVVYAQTPQAGGFWIGLPNGLTSAKAAAYLGN
                     GIRRTSKIGEYFIRPNAPAMFMSFAEVNFILAEAALRNLIDISPLTPKDYYEMGIDAS
                     YRQYAREIVENIPVYYPSFEGVSINIDDFITDFLNHNGAWDAANPLQKIATQKWVAMF
                     DQGLQAWFEWRRTGYPVLTPAEDGLNGGKIPVRLRYPSDEYSRNGENVAKAVALQGDD
                     NLNTPVWWDCN"
BASE COUNT         9140 a         7406 c         7661 g         9099 t
ORIGIN      
        1 atttgtgggg caaaacattg gtgccaacaa gattgacagg gtaaaaaggg ggctacggtc
       61 aaccctgaaa atttccatta gcttttgtgt tgtcaacatt gccattattc tgatttttgg
      121 ccacagcctg atggggatat tcactaaaag ttccgatgtg attgccatag gttaccagta
      181 cctgctggtt gttagcttgt tctacataat ttttgccgta atgttttcca tcaacgggtt
      241 gctcagaggg gtgggcgctg ctgttgttcc catgtacatc accctgctgt cgctctggat
      301 ttttcgaatc cctcttgcct acctgttgag caaaaccttt ggtatgggtg cattgggtat
      361 ctggtggtct attcctattg gctggacgat gggggccatt gctgcagtag cctactatcg
      421 ccatggaagc tggaagaata aatccctggt aaaggtacgc cagcaagctg cggattagag
      481 ttgagatggt agcaagtaag tagctccgaa aaccaaaagg ttgaatatct caccgaaaca
      541 aaactatttg ccatttttgc ttggattgaa ttccattttt tatcctaaag ctggttaata
      601 aattagatgt tgatggctgt ttcctaaaaa ttttatattt gtaggagcaa tataatggca
      661 ttccattaaa tactcattaa gctacactgg ttgataatga tggtaattca tgtcaggcaa
      721 catgatttca taaacgggtg atggagttct tgagttctat aaaactattg tgggtgcttt
      781 tcaatctggg caggtacttt tccttgcgtc aatagttttt atttattggt ttgctgccgg
      841 gtaaattcta tcatataaac tttttaaaac atgctaaagg actatttaag cagaattcaa
      901 gataccatta acaagggaga tgccagggag gagagctact actcacatct agatagcctg
      961 attaaagaat ttgcaataga aaatggcatt aaaaagattg atgtaacaat acttcccaag
     1021 aaaacagaag cggggaatcc cgatttccga gtatgggacg ggaagaacca tataaccggg
     1081 tatattgagg caaaggatcc ttcggttgac aatcttgata ggattgagga atcggagcag
     1141 ctaacacgat accggagcac ctttcccaac ttaattctga cgaattttta cgagtttagg
     1201 ctatacaggg atggcgaact ggtaagaaag gttacgattg gccgacagag gattgcccaa
     1261 aagttgaaaa cagctccacc ggtggagaac gagaaagaat tcaacgacct gcttggcacc
     1321 ttcttctcct tttccctacc acaggtacgt agcgcaaagg cgcttgcaca ggagctggct
     1381 aaacgtacgc gtttcctgcg cgatgaggtg gttagcattg agctggcaga ggagggaagg
     1441 attggaaaaa gggatattat ggggttctat gaggctttta ggaagtactt gataggcact
     1501 ttaacggagc agtcttttgc cgatctgtac tcgcaaacca tcacctacgg tttgtttgca
     1561 gctcgtaccc gttcagacaa cacttttaac cgtgagctgg ccttcaggta tattcccaac
     1621 accatcggca tcctgcgtga tgtgtttcgc ttcatctcgc tcgaagaccc tccaaaaccc
     1681 ctgcagatga ttgtggacga tattgcagag gttttgagcg ttacggatgt caagaaaatt
     1741 ctggacgact actaccagca ggggaaaggt caggatccgg ttgtccactt ctacgaaaca
     1801 tttctttcgg cctacgatcc ctccattcgt gagaaacggg gcgtttacta tacccccgag
     1861 ccagtagtgg gctatatagt acgctcggtt aaccaggtgt taaaaactca cttcaacatt
     1921 accgatggcc ttgcaggaga ggacgttacc ctgctcgacc ccgctgcagg aacgctcact
     1981 tttccggcag aggcggtaaa gctggcggtg gaggagttca ctcgaagtta tgggagtggg
     2041 ggtaaaacaa attttatcag gaatcatatt ctgaagaatt tttttgcctt tgagctgatg
     2101 atggcgccct acgccatcgg ccacatcaaa atgagtgtcc tgctcgagga gctaggctac
     2161 aggatgggcg acgacgaaag gtttcagcta tacctgacca acaccctcga aatggacgag
     2221 ctggagcaaa tttccatacc tggtctgagc tcactgagcg aggagagcca cctggccgca
     2281 agggtaaaaa aagagcagcc catcctggtg atacttggca acccgccgta tagcggcata
     2341 tcgggaaaca tcaaccaatg gaccgagaag ctgctaaaac aaaacctgga tggcgctcaa
     2401 agctactacg aggtggatgg aaagcccctg ggagagaaaa atccaaaatg gctacaggat
     2461 gattacgtga aattcctgcg ctttgcccag tggaaggtgc aaaaggcggg gttgggtgtt
     2521 gtgggcatga ttaccaacca cagctacctt gacaacccaa cctttcgcgg gatgcgccag
     2581 agtttaatgg gtaccttcaa cgaaatattc atactcaacc tgcatggcaa tagtcataaa
     2641 cgggaaacct ctcccgatgg tagtaaggac gagaatgtgt ttgacatacg tcagggtacc
     2701 gctattgcca ttttcgtgaa gcagaagggt aaaaatgggt gcagggttta ccataaagat
     2761 ttattcgggt tgagggaggt caagtatggt tggctaggtt ctaacgagat taaagttgat
     2821 gattaccgtg tgctaaaacc caaaagtcct tggtattttt ttattcctaa agatactgat
     2881 agcattcagg actaccttgc atggaagaag gtaaacgaaa tattcccggt aagtgtaact
     2941 ggcatagtta cagccagaga taatcttgct attgacttta atttggatgc attgaaaaat
     3001 aaaattatga tgttcagaaa tttaaaaatt gatgataata ctttaaaaca ggcatttaat
     3061 ttaacagaca attatcaatg gaaattagaa gagcagagaa aaaaaattca aaaagtaaca
     3121 gactggcaag aaaatttcac caaaattctt taccgtcctt ttgacaccag gaacatttat
     3181 tatcaggaaa atattgtttt tcggacacgg agggaagtta tgcgtcacat gttaaaagat
     3241 aatattgcca taaatacggt taggcaagta aaaaccggga aatcatggca gcactgtctt
     3301 gtttctgata gaattaccga gagctcatac ctatctaaca agacaagcga gataggttac
     3361 gtttttcccc tgtacctata caaggaagtt gcctctaaaa aatcccattt ccagtccatg
     3421 ttggtgttcg aaccggaggt agaatatggc ggtaaccgga cgcctaacat cgacaagaaa
     3481 attcttgaaa agctgaacaa atcctaccgt aaaaacccaa ctccagaaga aattctttac
     3541 tacatctacg cggtattcta cagcaacgtg tacaggagga aatacgcgga atttttaaga
     3601 atagattttc cacgggtccc atttacaaag gattatgatg tttttgccag aatggcagag
     3661 ttgggaaacc aattggccga tttgcacctg cttaaaggca aattattaga aaatcccatc
     3721 tctaaatatc aaggtgaatc tgaaaatgac agaattgaag ggattgccta tgacgaacaa
     3781 aagggaagaa tctatatcaa caagaataag tattttgatg gcattacccc tgaagtttgg
     3841 ggttacgaaa tcgggggcta tcaggttttg cagaaatacc tgaaagacag gaagggaagg
     3901 aatatggatg attcaaagca ttatagccgt gtcattaccg ctatcagtgc caccattgag
     3961 ctgcaacggc aaatagacga aacataccca ttagtcgaaa acgagctggt ggaattttta
     4021 tctgcatgaa ttttttggct tcttaaattt tctttaccgc aagcgcttgc tcagcataaa
     4081 tcgtttggct tcaataatct ttcccttccc ggctgggtgt ttttttaatt ttttatacct
     4141 ttgcagttca tcaaattaat agtttcaccc atggcaagta gaaaagatct taaaaaggat
     4201 gttgatagct tggttggcga ggttgttaac gattgcttca cctatatgac gctgcatggc
     4261 gataaaaatc gcgacaaggc agagggaatt atctcaaaaa ttatggaaag ccgtaacacc
     4321 gctattaaaa aaatcaacaa tccgggcaag ttgactgaca aaaaggctac aaaggcccac
     4381 ttcaaagcct tacagcagga gttgctggtt tcagccgatg agtgtttcac cgagttaagc
     4441 aagctgccta aggatttagg atagcagctt gagtttaatg agcaactatt tccccggaaa
     4501 agtagccggg gaattttttt tatgtcctat tgcgataatc gcgctgcccg gtgtgaacaa
     4561 aatttgtcag gagcggttta ttgcaaaagg gtttgggttg tctggtttat attttttgcc
     4621 gtaactttag gttaaagttt tttcggatga aacccctaaa tcgaatattc atttttttgc
     4681 ttctgctttt gtctgtggca aaaccatcta gtgcgggcag ggtggtacct aactcgatta
     4741 acaggtcatc tatcagggaa attgaagcct caattcgcaa tggagactat ggtaaggtta
     4801 ccagcatcgt ggttttaaac cggcaaggtg ctattcttta cgaaaagtat tttggattct
     4861 ccacccggaa ttcacttcat accatcagct cggttaccaa gagtattacc tcgctaatgg
     4921 tgggaatatg cctcgacaag ggctttataa gttctattga ttcacctatt tacagctatt
     4981 tccccgagta taaggaggtt tttgagaaag atagcaccaa aaagctcatc actctgcgcc
     5041 atttgcttca gcagactgca gggttgaact tcaaagagtg gcagttcccc tataactatg
     5101 cctctaacag ccttatggct accattgagg aaaaccggag ctgggtggag cgctttttct
     5161 acctgccggt tgacacgcta ccaggtgtaa aattttctta caacagcctt gcctctcagg
     5221 taatagctga ggtgctctca cgtgcctctg gaatagctta cgatgagatg gttcggaaat
     5281 ttttattcaa gcccctgtct attgaaaatt tcttgtggga gagctatgca gggaatagcc
     5341 tacctgcttg gggaggattg tccttaacta ccttcgatat ggccaaaatc ggtttgctgg
     5401 taattaacgg gggtatatat ggcggtagga ggttggtttc cagcgaatgg gtggagagtt
     5461 caacggtggg cagggtgagg gttaacgata acatatacta tgggctgcac tggtgggtag
     5521 atattttacc atcgggaaaa ccattgccat ttgccgccgg gtatggcgat cagtatgttt
     5581 ttactgcgcc cgataggggt ttggttgttg cgcttaatgc gcagaacttt tctgactttc
     5641 gcttctccaa gtcggcaatt gacctggcaa atattttact atcatcggtt gaagtagaaa
     5701 atttccaaaa ttaaaaccgt atgccatgag tgacctgaaa actagctaca tgggaataga
     5761 gcttaaaaat ccgctaattg cgggtgcatc gtctctgtcg ttaaacattg aatcgttaaa
     5821 ggcaattgag gccgcaggtg caggagcagt tgtcttcaaa tcgcttttcg aggagcaaat
     5881 tcaactcgaa gcgctggagc ttgaaaatga gctggaagag tataacgagc ggcatgccga
     5941 aatggttaaa ctctttccca cgcttaaaca cgcaggccct gccgatcatc tggctaaact
     6001 taggaaggcc aaggacacgt tgagcattcc agtcattgca agtcttaatg ccatgtatcc
     6061 cgacacatgg gttgaatatg ccaaggagat tgagcaaaca ggggttgatg gtatagagct
     6121 gaacttctac actactccca aaaattttga ggtagagggg aaaaccatta ttcaatcgca
     6181 agttgacacg ctgaaaagtg tcctcgaaaa ggtgaccatc ccggttagcg ttaaacttag
     6241 cccgttttac gccaacccac ttgatgttat tcgccgcttt gatgagacag gtattcaggg
     6301 tttcgtcctg ttcaatcgtt tttttgagcc cgacattgat attgaaaagg aggagttggt
     6361 acagaccata accctgagca atgaggatga cctacgtttg ccattgcgat ttaccggttt
     6421 gctttatggc aacatagtag cagacatttg ctcctcaacg ggaataatga caggtgctga
     6481 tgctgtaaag atgttgcttg ccggagcaac tacgtttcag gttaccagca ctatttatgc
     6541 gaatggtatt gagcaaatta ccaaaattct gggtgaaatt gaggcatgga tgcatcgtaa
     6601 gggttataaa tcccttcatg atttcagggg taagctggca aagaaaaatc tgcacgatcc
     6661 ctttgcatac ctgagagccc agtacgtgga tatccttcta cgttctaacg atatcataaa
     6721 agactctgca atctagccta aaattgattt ggagattaat cgaatttgct ctacagaaaa
     6781 atatcacttc tagcaggtat taatggaggg gtgattgccc aagatataat tatgctgtag
     6841 cctgttttac ccgataactc gttatgaaac agttgtaatt actacattct tacccttgca
     6901 atttgctaga gagaccaagt tgtttgtaaa ttctctactc cacgtaaaag tcgttccact
     6961 ctttccttac cacctcgttg gtttcaacgc tgtccactct ggaccgttca ggccctttcc
     7021 agcaaagacc aatcagctca ttaagattct cctcctgtcc ctgagcctcg atgtacacgc
     7081 ttctatcggg catattcttt acgaaacccc tgatgcctag ccttttggcg ttgcgtagca
     7141 cgaaatacct gaaccccacg ccttgaaccc taccttttac acttatggct tttgttttag
     7201 ctgtcatggc tggttttgat tatacaaatt tagggctatc tttgtttgta ttcaatgctg
     7261 aaaggatttc atggagaaat ttttgaagca tttttgaagt agaacaaact aaatgagtgt
     7321 atggtagtag attatgtcct gattgtttta gcggctgccc ttgtggttgt tggattggtt
     7381 ggttgttttc tacccgtcat tcctggccct ccgttgagtt gggtaggatt actgctggcc
     7441 tactttacca actttgtgaa ttttgattgg agcttgcttt tatggacggg gatagccacg
     7501 cttgttgtag ttattctcga ttatgtaatg ccggtgtggg taaccaggaa aatgggtgga
     7561 accaagcggg gagtatgggg ctcggctatt ggattggtgg ttgggctttt tgtttttcca
     7621 cccctaggaa tcattgttgg accatttgta ggcgcattca tcggtgagct caccgcaagg
     7681 gataaaagca ataacccgct gctctcggca acaggttcat ttataggatt tcttgtaggt
     7741 accgggctaa aatttgctgt ttcgggttat attgcctacc gctttacggt ggaggttttt
     7801 ttcaggtagc ccaaaagaca ttattgattt tccttttaaa ctccaaataa acataatggt
     7861 aaaaccacac caacagcaca tcgaatgtta actgcatgga aaaaatcgga attcaaactt
     7921 accaggagtt ggttttacct ttaagcccgt ggttagtcct tctgctttcc ttctgttttt
     7981 gaatcatcct tcctaaaggc gttattcacc tttatgtttc tacccttaac ctgggtatcg
     8041 ttcagagcgc tgatggcttt ttggccatcc tcatcctttt ccatttcaac aaaaccgtac
     8101 ccctttgatc ttccggaggt tttgtcggca ataattttaa ccgagagcac gtttccaaaa
     8161 ggggcaaata gctctttcaa gtcatctggc gtcattgagt aggcaatgtt accaacataa
     8221 atcatcataa ggcataaaga tttggttatt atgcaactaa aattatagct aaaaaaatgt
     8281 aaatgcaaga aaaaagcagc ttttttaagc tgctttcccg caaaaaaatt taaaagccta
     8341 gctgccaagc gttgccacca ttacagcttt aatggtgtgc accctattct cagcctcatc
     8401 aaaaacaatt gaggcatccg actcaaaaac ctcatcggta acctccatgg attctaatcc
     8461 gaatttcttg aaaatatctt cgccgactgc agtctcgcgg ttgtggaagg agggcaggca
     8521 gtgcatgaac ttgcagtttg gattgccggt gagctccatg gccttgcggt taacctggta
     8581 gggttttaag agcttaattc gctcgtccca aacagaatcg gcttcaccca tggaaaccca
     8641 aacatcggtg tagaggaaat cgcacccttt aacgccctct tctacctttt cggtaagggt
     8701 tatccgggct ccggtggttt tggcaatctc catgcaggtt tttaccagtt cttcttttgg
     8761 ccagcaggcc ttaggcgcac aagccctgaa atccattccc attttagctg ccccaaccat
     8821 aagcgagttt cccatgttgt ttctggcatc gcccatgtag cagaaggtaa ctttgctcag
     8881 cggtttgctg ctatgctcgc gcatggtaag aaaatcggcc agaatttgag ttgggtggta
     8941 ctcagtggtt aggccgttcc atacaggaac acccgcatac ttggcaagag tttcgacaat
     9001 ttcctgggag tagccgcggt actcaattcc gtcgtacatt ctgcccagca ccctggcggt
     9061 atccttcatg gattcctttt tccccatttg cgaacctgaa ggtccaaggt aggttacatg
     9121 ggcaccctga tcgtaggctg ccacctcgaa ggcgcaccgg gttcgggttg agtccttctc
     9181 aaataggatc acaatgtttt tcccctttag ctgctgctgt tcggttccgg cgtactttgc
     9241 ctttttaagg tcggccgata ggtcgagtag aaactgaatt tctttcggag taaaatcaag
     9301 aagttttaaa aaactccgat tccttaggtt gaatgacata ctcttaagtt tttatgttgt
     9361 tttacaattt atgataagct taaaatgcac tgttgaaatc atggtttcag gttcgcccgt
     9421 tcagctgcat tcctggaggg ctatgccttc tctaaaagtc ttatagttcg aacggtatgc
     9481 ttaccgggtt agcttgatac ctagtcctcg taggccatgg ttattttagt tccgtagctc
     9541 atatcctcca acttggtggc ttcggttatg acactcattt tgcctccatt ctcgatgaag
     9601 ttcaaacagg cacgtatttt aggtgccatg ttgccctcgc caaacatccc ctcctggagg
     9661 tatctggttg catcggcatg gttgaggaat tccaacttct tctcgttggg tttcttgtag
     9721 ttgaggaaca caaatggcac atcggttaga atgtagaact cgtcagcctt gatttcggca
     9781 gccattagcg ctgatgcgga atccttgtca ataaccgcct cagcaggcct cacatttttc
     9841 tcctcatcaa tgtaaacagg cacaccacct cctcctgagg caataacaat tattccctga
     9901 ttaaccagct ggtctataac cttgatattc aaaatccctt ttggctttgg agatggaact
     9961 accctgcgcc aaccactacc cgtatccttc acctcttcct taaaaatcca tcctttttgt
    10021 gaggccaggg catcggcttg agccttgtcg tatattcgcc ccacacgctt ggttgggttg
    10081 ctaaaggctg ggtcgttctt gtcaacaatt accggtgtta ccagacagca aacctcctta
    10141 tcaatgccgt gatttttcag cacgttacgg agcatgcgtt caatcatgta tccaattcct
    10201 ccctgcgaat cggcaacgca tacatccagg ggcatttggg gaatgccgta tagctgctct
    10261 cccgcatcgt tccgcatgag gatattgccc acctgagggc catttccatg gctaatgaca
    10321 agattgtagc cttccttaat cagataaacc aggttctcca gtgtttcggt tgtgttttgc
    10381 tcctgttctt ctattgtccc aacctgattc ccccttagga gggcattacc tcctaaagca
    10441 acaactgcta atttgctcat atacaaatta ttaatataat gttgatttaa aattgcaaag
    10501 ctattaaatt ttttcaaagg tattttaccc tgccatcttc aggcttgctt aatcaatagt
    10561 tttgcagtat gtttaataac atatttttta cagctgcttt tcatcacctc ttttttagaa
    10621 aggttcgaga ttgttttctc cgaatgctga ctcgctggaa gtgaaaaaag ggggattagc
    10681 cagctagctt gccttttggg acatggccta gtttattatt gcaatagttg tatatatggc
    10741 ggctatttta ctacttttat ggtttgttaa ggatgatgca tatgaggaag ccaattgccg
    10801 gtttggtcat tttgtctgct tttattcttt gttcatgtgg cggtagggtt aaaaacaata
    10861 gcatttcaga gggtgttatt acttatgaca tcacctacga tagcgcaatt cagagccggt
    10921 atgatgcatg gctgctacct tccgagttga tagttaggtt taacagcaac cattccataa
    10981 attccattga ggctctaggg ggcgccattt ccatttcaat tattaagaat cctgaacgta
    11041 atgaattttc gacactgctt aagattttta ataaaaaact ttaccacagc gaacccatag
    11101 aacaggagcg ttacccggca ctttactccc gaatgcccgc ggtaaagttg atttcggagg
    11161 gtgaaacatg cgaatttttg ggttatagct gcactaaagc taaaggctac tttgccgata
    11221 atcccgattt tccattcgaa attatttaca ccagccagat tcaaatcgaa aacccaaatg
    11281 cccacacacc ctttgaggag gtaaagggta ccctgttgca gtttaacatc aggctgaata
    11341 accttaccat gcacgccacc gcaaggtcgg taaagtccga gcatgtcccg cttgagctgt
    11401 ttgctcctcc atcggactat gttgttaccg attcacaaac gatagccaat ttaatctaca
    11461 cgcttcagca gtaagtttgt aaatctaccg gtaagagttg aatgtaggtt gttccccaaa
    11521 agtacattaa cctttggatt ttgtcggatt ttattcatta cttttgcatc ttgtcttaat
    11581 gatgtagggg ataatggaag ttggacaact gcaaccaggg tgcttaaacc gtaaggagat
    11641 tctgccagtc atgaattctg tccttttgcc ccagctcttg ctttaaaaat aaacgattca
    11701 gggatggcat cagatagcaa ccggccaaat gtaaaggaaa agaacaacgc cacaaaggag
    11761 gggcaggaca acgggaaaaa taaacttctc cgggacgaaa gggtaaggtt taccattggg
    11821 ttattcttgc tgctactggg tgtttacttt cttatttccc tgatttcata cctgttcacg
    11881 tggaaagttg accagagtct ggagtggcaa agcctttttt ccaaagccga atacgaggtg
    11941 aagaactgga ccgggaggtt tggagcggtt atatcggctc agtttatgaa ccactggttt
    12001 ggcctctctt cacttgcgct gccatctatt atcatagttt ttggacttaa gcttcttaag
    12061 ctgtacagaa aaaatatctg gcggtttatc tttaatatct tcataggaac actactatgc
    12121 tcgctagtgc tgggcttttt atttgaaacc gctaagggat ttcttggtag cgggctaggg
    12181 ggagcgcaag gactttttgt gtcgaaatgg ctgtcggcat tgctgggtaa ggttggaacc
    12241 tcttttttgc tgttacttct ggttattgcc tacaccattt ttatcaagcc ttcaacgcta
    12301 cactggttca gaagaaccat caggggatta cttattagaa tgaggcgccc ggtttcccga
    12361 aaaaatgata aaaggcaaaa taaaaaagaa aaaactgctg aggagttggc tggacaagtt
    12421 gactctgaag ttctacctaa taacaccgat gccgctgttg aggttacttt ggaaaagttc
    12481 attcccgagc ctaattcaga attggttgat gctgagccat tcgagcttat cgaagaggag
    12541 acagctggca ctaacagttc aaatgcagaa aatactcccg aaaaatccga tgtggagttc
    12601 accattgaaa aaatcaacga ggtggagcta aacccggttg aggtgaacgg acaggagcta
    12661 tacgacccca ctttagagct ctcccgctac cagcgtccgc tgctggagct gctggaggat
    12721 tacaaaaaca ccacctcgcc cgtatcaaac gaaacgctta ttgaaaataa gaaccgaatt
    12781 gtagaaaccc tgtcgcacta caagatacag attgataaga taaaggcaac cataggcccc
    12841 accgttacgc tttacgaaat tgttcctgca cctggaatcc gcatcagcaa aattaaaagt
    12901 ctggaggacg atattgccct tagcttggct gccctgggca taagaattat tgcgccaatt
    12961 cccggcaagg gaacaatagg tattgaggtg ccaaatcaga accccgaaat tgtttcgatg
    13021 cactccatca tcaaatcaca gaagtttcag gagtgcaagt atgaattggc catagcttta
    13081 ggaaaaacca tatcgaatga gatattcatg ctcgatttgg ccaagctacc ccacttgctg
    13141 gttgccggag caacaggcca gggtaaatcg gttgggctga acgccataat cacctcgttg
    13201 ctttacaaaa agcatccggc ggaaatcaag tttgtgctgg ttgaccccaa aaaggttgag
    13261 cttacccttt acagcaagct ggaaaggcat ttcctggccg aacttccagg ttcggaagaa
    13321 cccattatta ccgatacgca aaaggtaatc agcaccctca actccctttg catcgagatg
    13381 gataaccgct acgagttgct gaagctggcc caggtgagga atatcaagga gtataacgaa
    13441 aaatttgttg agcgtaggtt gaaccccaac aaggggcacc ggtttctgcc ctacatcatt
    13501 ctgataattg atgagtttgc tgatttgatt atgactgccg ggcgggaggt agaaacaccc
    13561 ctggcccgtt tagcccagct ggccagagct attggcattc acctgattat tgccacgcaa
    13621 cggccgtcaa ccaacatcat taccggcgtt ataaaggcca acttcccagc acgtatagcc
    13681 ttcagagtcc cttccatgat agattcccgc acaattcttg accacccggg ggccaataac
    13741 ctggttggcc gtggcgatat gctcgtttct gccggaagcg acatggtgag ggtgcagtgt
    13801 gcttttatcg atatccctga aattgaacga ataaccgatt ttattggtaa ccagcagggc
    13861 taccccgaag cctaccagct ccccgactat gcacccgaag ggcaggacgg ctttagggag
    13921 atagacctga ataagcgtga tgagcttttt gaggaggctg ctcgcatggt ggttcaaacg
    13981 caaattggct ccacctccct aattcaacgt aagctctcga ttggctataa ccgagccgga
    14041 cgaatagttg accagctcga agcagccggt atcgttggcc cgtttgaggg aagcaaggcc
    14101 cggcaggtgc tgattgccga cgagctgtct ttggaacaaa tattgagggg tttatctaac
    14161 cagtgagcca caccagctgt acaccttgat ggctgctaaa tggcgatatt tcgtttaata
    14221 attaggctat gagaagttta atgctgtttg ttttcgccct attatcggta aacctcgttg
    14281 cgcaggaatc ccaggatgta ccacaaactt tttgggattt tagcagtaaa atgctggaag
    14341 ccagtagtat aaatgccact ttttcactta ccatcgagaa ccgccaggaa aatgttgcca
    14401 acacccagca gggtgaaatt ctgattaagg gcaacaagta cctgctgaag attatgggca
    14461 tggaggttta ttttgatggc tcaaccaagt ggcagtacat tggcgaggcc aacgaggtta
    14521 ccatttccac ttccggtggg gagttggaca gccctcttga tgaccccgct ggcattttta
    14581 gagattatga atccaggttt aaggtgagat ttatatcgga aaccacggtg aaaaaccgca
    14641 cgctggtcga aatggacttc tacccaaaag cccttgactc tccctacgtt tcggttaagc
    14701 tgagatttga caaggtttcg ctcgaaccgg tttttatcag ataccatgga aaggatggga
    14761 caagctacat cgtcaacatt agtaaattta aaaccaacgt ccctgctccc gatggcacct
    14821 ttacattcga tatttcaacc cgtaagggca tagaggttat agatttacgg taggttggaa
    14881 ttttttcttc tgtactcatt agcattacat agttatttcc ttatgactag ctgtacacct
    14941 gcaacaattt ggctaacagg actaccctcg tcgggtaaat caacaatagg gttgggctta
    15001 ggccggatgc tcgatggcat tggcgtagga tatgtgcttc tggatggtga tgaggttcgc
    15061 agcggcgtat gctccgacct gggcttttcc gatgacgacc gaaaggagaa tatccggcgg
    15121 gttgcccata ttgccaagct gctcaattcc caaggattag tggcaatttg ctgcctggta
    15181 tcaccgcttc aatccatgag ggatttggcc agatcaattg tgggcagtag gtttttctac
    15241 gaagtattcg taaatgccag cctcgaaacc tgcatgaagc gtgatgttaa aggactttac
    15301 gccaaggctg aacaaggaca gctaaataac ctcacaggtt tttcggcagc gtatgagaat
    15361 ccaaccgatc caaatcttgt catattcaac aattcaatcg gggtagagga ggcggtaaat
    15421 atgctgttta cccatgtggt tgactggctg gagattccat ggtaggttga acccttttgt
    15481 ctgtttgagt gttaaggtta taggttgtaa gtgagcatga gctgcgcaat cgttgttata
    15541 tttgtgctga aaattttaaa ggagaagtaa gtcgttgata tgagtaaatt caagctaaat
    15601 catttacggg agctggagtc cgagtcaatt tacgtgattc gagaggttgt ttcgcagttt
    15661 gagaatcccg ttatgctgtt cagtgggggt aaggattcca tcgtgatgtt tcaccttgcc
    15721 cgcaaggcct tttatccggc caaagtaccg tttcctcttc tgcatattga tacgggtcat
    15781 aatttccccg aaaccctgga gtttcgcgac tggctggtta aggaaacagg ggtgcagctt
    15841 attgttcgtt acgttcagga ttccattgac aggggtaggg ttgccgagga aaaaggctac
    15901 aatgccagcc gtaacaagct tcaaaccgtt accctgctcg atgccattga ggagttgaag
    15961 gttgattgcg ccatgggagg gggacgtcgc gacgaggaga aagcacgggc aaaggagcga
    16021 ttcttttcgc accgcgatga gtttggacag tgggacccca agaaccagcg gccggaactt
    16081 tggaacctat tcaacgggcg aaagaatctg ggcgagcatt ttagagtttt ccccataagc
    16141 aactggaccg aaatggacgt ttggcagtac gtgctaatgg aacagattgc cctgccctca
    16201 ctgtactttt cacacgaacg cgacgtattc atgcgcgacg gggtcctcta tgccaaaacc
    16261 ccattcctgc cgatgaaaga tgacgaggtg gctgaaaagc gtattgtgcg gttcagaacc
    16321 ataggcgatg caacctgtac cggtgcggtg gagtctgcgg ccagcacgct tgaggagatt
    16381 atccgcgagg tggctgccac ccgaaccacc gagcgcggtg gtcgtgccga tgataagcgc
    16441 tcggaagcgg ccatggaaga caggaaaata gagggttatt tttaaacacc ttactattta
    16501 cggaaactta cattcgcaag aattgtttca tggcagtaag attaagccaa tacaggtttc
    16561 aaaatattta acttgataat ctttttacta aaaattgatg gaacaggaaa ttaccagtag
    16621 cgcgtacctc gacatggagt tgctacgttt taccacggca ggaagcgttg acgatgggaa
    16681 aagtacactg attgggcgcc tgctctatga ttccaaggct atctttcagg atcagctgga
    16741 ggctattgag cgggcaagca ttaaaaaagg tgaggattcc ttagacttgg cgctgcttac
    16801 cgatgggctg cgtgccgaga gggagcaggg tattaccatc gatgtggcct acaggtactt
    16861 tcatacccct aagagaaagt tcatcattgc cgatacccct ggccatactc agtacacgcg
    16921 taacatggtt acaggggcat caaccgcaaa tgctgctgta attctggttg atgccaggca
    16981 cggtattgtt gagcagacca ttcgtcatgc ctacattgcc tcgcttctca gaatacccca
    17041 tgtgattgta tgtgtgaaca agatggacat ggtggactac gctcaggaat cctttgaggc
    17101 cattcagcgt aggttcctgg aattttcaag ttttcttgac ttatacgatt tgcggtttat
    17161 tccgataagc gcgcttttgg gcgataacgt ggtggagcgt tcaaagcaca tggattggta
    17221 tcaggggcca acgttgatgt acttgctcga gaatctctac atttcgaacg actacaacca
    17281 gctggatgcc cgttttcctg ttcagtatgt tattcgccca cagtctgcgg aatttcccga
    17341 ttaccggggc tatgccgggc gcatggctag cggaacgttt agggttggag acagggtgaa
    17401 ggtgctaccc tcggggttta gctccgtgat taagggcatc gacttctgga cagacagcct
    17461 cgactccatt accgccccgc aatccggaac cattcttctg gatgatgacc ttgacatatc
    17521 gcgtggtggc ctcatcattc ccgaggagaa tggcatcaag caggggcagg aggtggatct
    17581 gatgatttgc tggttttccg aaaaaccact cgtggtggga ggtaagtatg ccgttaagca
    17641 caccaccaat gacctgaggt gtctgattaa ggaggtaaag tacaaggtaa acatcaacac
    17701 cctggagaag gattaccatg aaaagagcat tggaatgaac gatatagcct gcattacaat
    17761 taaaaccacc aagccgcttt tctacgattc ataccggcaa aacaggataa ccggcagcct
    17821 gataattatt gacgaagcca caaacgttac tgttggcgct ggaatgattg tgtagctaaa
    17881 taccaatgtt gcttagagcc ttctcaagta tttcggcatg gtcgaatgcc agcggtggaa
    17941 gattattaag gggaaaccag ctagcagtag cggcatcgtc tccagcattt ggcaaagggc
    18001 tatcagcaga ggcaaatcct aagtaggcaa cagaaatgtt gcggtcgcgg ggatctctat
    18061 ccaccctgtc gaaagcccct acctgcttca acgttattcc gtcaagccca gtttcttcct
    18121 ttaattcacg ggcagctgcg tccgaaagca gctcattcat ctctggaaat cccccgggca
    18181 atgcgtagca acctttatag ggttcgtttt tcctttctat gagcagcaag tcaacagggt
    18241 ggccttgagt atcggtcctg aagatcactg cgtcaactgt taccagcatt cggggatatt
    18301 cgtagcagta catttttcag cttattacat ggacaactca tcattatttt catcgtcatc
    18361 tccctgattg cgccgcttat cccttatttt aaaggcaatc cagactgcaa taaggatgat
    18421 gacaaagacg atgactatct gatttttcag catggatttc tttttggtaa atataggcct
    18481 ttggaaaaag attatcaatc aaaaattact cttcggataa ttcttagctt atgggtgtag
    18541 cggtgggctt cgaggttaaa tatgccttgg tgatcgaacc tgtccacacg tacaaatccc
    18601 gaacaatgta taatggtggc atcttcggca acaataccga catgggtgat aaccccattt
    18661 tcatcgtcga aaaatgccag atctccaggt tttgcttcat tcgcaaattc tatggtttgg
    18721 ccaatgccag cctgatcgcg ggcatcacgg ggcagcatag ttccggctat ctcgaaaacc
    18781 acctgcacaa ggccggagca gtcaactccg aaaagtgatc ggccacccca taggtagggc
    18841 gcatccatga aggtggtgga cagcgcaaga atttttttgc gtgggttgtc gggcaacttg
    18901 gcgaatgggg tgtccatgca cacaaagctg gagctgccat ttctgaaggt ttttccatca
    18961 aaggagtgca gctgcgatcc tggacaaagg taggttgtga atccacttgc agctttcgcc
    19021 ctgaagagcc gactggcaat tttaggtttg tcggtaaaga ttggcgagtt gtctggtagc
    19081 tgtagcgata gcctttcgtc catccatcct gaatacccgt cggtatggcc tttaactttt
    19141 aaccatccac cctggcgctc aaccatgtcg aaggtctctc caaaaagcag ctggttcacc
    19201 atttcgctgg tgtgggttgg ttcgcgacgt aacgctgcca tactttttga aactactccc
    19261 ttttccatac ctagaaaatt ttggtaaaaa tagaaaaata ttggtcggct ttgtaaagca
    19321 gtatcgggat agattgaatc gtttataacc gtctaaagca aaagggttta aaaagcaccc
    19381 taagggatgg ttcttcaagc tatatgctgg caatgccttt gtccgtttaa agtatgaata
    19441 tgagcattat tgcatatcgc aattcgacta attaggatta cttttgctga aaattttttt
    19501 attgcaacac caacacgagc tatataagct ttacctaaag catccttcgc tgaagcagct
    19561 tttggatgct ggtcagaatc tggagggcgg ttttttcctt aaaggccttg ccgggtctgc
    19621 ggttgcattt gtactgtccg gttacgttga gcagaggcga cggccatcgc tggttgtggt
    19681 aaacgataag gaggaagcag cttatctttt tagtgatttg tcgtgtctgc tgcccgaaaa
    19741 tcaagtgtac tttttcccat cgtcctacaa gcgttcggtt cagtatgggc aaactctccc
    19801 cgatgctatt gttcagcgga ctgacgttgt agcgctcctt ccagctctca acgaaaatga
    19861 cgaaaggcct gttgtagttg ttacctatcc cgatgcccta tttgaaaagg tggtgagcct
    19921 ggatacgctc agccgtaaca gcataaaaat ccagaagggc gaaaaagttt ctcccgactt
    19981 catttgcgat acacttacag agtacggctt tcagcgttcc gattttgttt atgaacctgg
    20041 acagttctcc gtaaggggga gcattgtgga tgttttctca tactctgctt ccactcccta
    20101 taggcttgat ttctttggtg atgaggtgga aactattagg tcgtttgacg tggaagacca
    20161 gctatcgaag gagctgctca gcgaagtagt tattattccc aacctgcaat cgcccgagga
    20221 caacccttca aggattctgc tgaccgattt gctgcctcca agcaccacgc tctgggtaaa
    20281 aaatccggaa ttggtgctga accggatgga tgagctaagc cgtattgttt ccacagcaac
    20341 ccagcttaac gccagtaact ttgcttcccg agacattttt tccaacagcc tgctcacccg
    20401 gtcaacccta tactttggta ttaaaccgtt gtccgtgcag ctacccacct tcgattttgg
    20461 cacatctccc caacccgcat ttgggaaaaa ttttgaactt cttgttcaaa aaatcaacga
    20521 ggatgccggg aggggctatc aaacttttat tctgtccgac aacagcgctc agctggagcg
    20581 gctcgatgga ataatgcacg gtattgcccc cgacattgcc tacaacccgg tggaagggac
    20641 catctcggaa ggttttgttg accataacct ccgaattaac ctgtacaccg accaccagat
    20701 atttgaaagg taccaccggt acaaactgaa acacgagttt aaccgtcggg atgccataac
    20761 catgcaggag ttggtagatc tacagccggg agactacgtg gtgcatatag accatggaat
    20821 tggaattttc ggaggtcttg tttcaactga gattaacgga aaaaagcagg aggctatcag
    20881 gctggtgtat caggataacg ataccctgct ggtaagcatt cataacctgc ataagatttc
    20941 aaagtaccgt ggaaaggatg ccgaatctcc taaaatctat aagctgggtt cgggtgcatg
    21001 gcagcggctt aagcagaaca ccaaaagaaa aatcaaggat attgcccggg agttgattgc
    21061 cctgtactcc cagcgaaggt cgcagcccgg tttcagcttt tcgcccgaca cctacatgca
    21121 gcaggagctt gaagcctctt ttgtttttga ggatactccc gaccagcttt ctgccactaa
    21181 ggcagttaag gaagacatgg aggctgaaag gccaatggat aggctgatat gcggtgatgt
    21241 aggatttgga aaaacagaag ttgccattag ggcagccttt aaagcggttg ccgatagcaa
    21301 gcaggttgcc gtgctggtgc caaccaccat tcttgccctt cagcattacc aaacattcac
    21361 cagtaggctc aaggggttcc cctgtacagt tgagtttctc aatcgcatga aaactcccgc
    21421 acagcaacgc gagatacgcc aaagggcatc cgagggcaaa attgatattc tgattggaac
    21481 ccatgcccta ctcaacagca cgctgaagtt taaagatttg gggttactcg ttgtggatga
    21541 ggagcaaaaa ttcggtgttg cagcaaagga aaagcttaag cagctaaagc tgaatgttga
    21601 taccctcacc ctaaccgcta ctccaattcc acgcaccctt caattttcac tcatgggtgc
    21661 tcgcgatctc tccataatca atactccgcc tgccaaccgc caccccatac taaccgaggt
    21721 gcataccttc agcacagcaa ttataaagga agccattgat tatgaaatgg ataggggcgg
    21781 acaggtgttc ttcgtgcata accgtattca aaatatagcc gaagtggagc tcatggtgaa
    21841 taagatttgc ccaaaagcca aaaccgctgt agcccacggc cagatggagc cctcgaaact
    21901 agagaaaatt atgctaggct tcataagcgg tgactacgat gtgcttatat caacctctat
    21961 aattgagtca ggattggata tttcgaatgc caataccatg attattaaca atgcccagat
    22021 gtttgggcta agcgatttgc accagcttag gggcagggta gggcgttcaa ataaaaaggc
    22081 cttttgctac ctgcttgccc caccgttgga aactttaaca gcagatgctc gtcgtaggtt
    22141 gagggctatt gaggagtttt ccgaactcgg cagtggattt agcattgcca tgcaggattt
    22201 ggatatacga ggagctggta acattcttgg ggcggagcaa agcgggttta taaccgaaat
    22261 cggttttgag gcctaccagc gtattttgga tgaagcactc gaggagttga gggaatcgga
    22321 atttgttacc attcaaactc aggccgaaca tgagacccaa agggaatgga agccttccta
    22381 tgccgattgc cacgtggaaa ctgacatgga aatacttcta cccgacagct acatcaccaa
    22441 caccaccgag cggattaagc tctaccgtga aattgatagc ctccataatg ccgaggagct
    22501 agaaaggttc aggaatgaaa ttcgggatag gtttggacca ctgccccggc aggtggaaga
    22561 tctcttaagc gttgtaagac ttaggtggat agccgttgag ctgggctttg aaaagctaat
    22621 actgggtaac ggccagctga tggctcattt catctccaac cagctttcgc cgtactaccg
    22681 ttcttctact tttgagggta taattcgttt catccagcaa catcccgagc gctttaagct
    22741 caaggaggga aaggataagc tgacaatccg tatagccgga gtaggtgaaa ttagtgaggc
    22801 aattgaaacg ttgcagaagc ttcatgaaac cttgcccgta aaatgaccca taaatttaca
    22861 catccgccaa tatattcctg ttatgtatga acttaattat tgatgttgga aatacgcagg
    22921 taaaggttgc cctcatggag cagggcgaga ttgtctttct gaaaagtttc tccaccctca
    22981 gcgctgggag catggaagaa attttatcta cctacctgcc ccataaggct atagtctcgg
    23041 ttgttggaaa ttttcagccc gaaatattaa gctaccttag ccaaaaactt atggtacacg
    23101 tgcttgggtc taagtcaaaa cttcctttaa aaaacaccta tgaaaccccg ctgactctcg
    23161 gttacgaccg gattgcggtt gccgtgggag ctattaccct ttacccaaat caaaacgtgc
    23221 tggttgttga ctgtggaacg gccattacct acgatgtggt gactagcgac gaaatctacc
    23281 tgggtggtgg tatttcaccc ggaatatcgc tacgcttcaa atcgttgaat gactacaccg
    23341 caaagctacc cctactaagc atttccaata ccttcagcct gattggtcgg aacacaaacg
    23401 actgcatact ttccggtgtg ctaaatggcg tggttgctga ggttgacgcg tatatcgaga
    23461 aatttaacct aatttatagc aacagcaggg tagttttaac aggtggggat tccagcttct
    23521 ttgtagataa gttaaaaaat agcatatttg taaacccaaa tttaatctat accgggttaa
    23581 accgaatttt ggaactcaat gcagaatagt agaaaaattt tacttctagt gggctttacg
    23641 ctagcagctc tagaagtttt ttcacaaaat atctatacct attcgcccta ctccaggttt
    23701 ggaattggca ttataccaac tcatgggttt agccaaacca gggcaatggg aggcgtttcg
    23761 caatcggtaa gaagccccta catgataaac taccttaacc cagcctctta tgctgctcag
    23821 gattccatgt cgttcacgtt tgattttggt ttggagagca acaattcatc gttcagcggt
    23881 tatgaccagt acctgaataa ggtatcggcc tcaagcagcg cagggggcat tcaccatatt
    23941 gccattgctt ttccggtgtc gaaaaaaatt ggaatggctg ctggcattgc cccatatagc
    24001 agcgtgggct acaggctaat tcgttttgaa accgatccat atattcttag cactattgga
    24061 agaattaagt acgagcatgc cggcaaagga ggcattaacc agctgtttgt tggtgcaggt
    24121 tacgctcctt ttaaaaactt ctcaattggc gctaatttca tctactactt tggaagcctc
    24181 gactacaaca acaacataat ctttccgtcc tcgtcctatt cctacactaa tgccagtgtg
    24241 ggctcaagca ttatggtcag cgatgtatcg tttaacgtgg ggttccagta caggcttttc
    24301 ctagacgagg ataaaaacaa ctcgttgggg tttggcgtta ccgtggatgc gggttcggat
    24361 atcaacatga agtataagtg gctaaccaag ctcgaagggt caacctacgt tgattccctg
    24421 ataccacatc cccaatctaa aagtaaattc aaacttccta caggtgttaa ctcaggtatt
    24481 tcctacacgt ttaaggataa gctctttgca acagccgaat attactatca ggattggact
    24541 aaaacggaag cttttaacac cacccttcag atgggaaaaa tggaagcctt ccgtttgggg
    24601 gttgagttca cccctaaccg tagcgatttg aagagctacc ttaaaaaaat cagctatcgg
    24661 gcagggggat tttacaataa gactaacctt attatcaact ctaggcagat tgaagaatat
    24721 ggaatttccc ttgggtttgg cttacccttt aggaataata ccagctttaa cctgtcggcc
    24781 gaacttggta ggcgggggac aaatgaaaac tatcttatta aggagtcgtt ctttgtcctg
    24841 aacctgggca ttacctttca cgattcacca tggttcttta agcgtaagta ctactagcag
    24901 gtaaactctt tagctatgcg catcacacta tttaagggtt acaagtacat actgccaggc
    24961 attgccatag tgggtactac tttctttttc ggctgcaaga ccgatactga agccatccag
    25021 tacttttcgg ataagaaaag tatacctggc gttaccgcct acaactcgga acttgagtac
    25081 accgaaaatg gggtggttaa aattatggtg aaggccccta ttaccatcta ctaccagttt
    25141 tccgaagaac cctacaccga atttcccgag ggtattaccg tatataccta tgatgatacc
    25201 ctgggtgtag agtcgaatct gactgctaaa tacgccattt actacgaaaa aaaaatgctt
    25261 tggcatgcaa agaataacgt tgtggcacga aaccggaaag gggaggtgct taacaccgag
    25321 gagctctact gggaccaagg caaacatctg atttatacgg atgtgaatgt aaaaattaac
    25381 agcgtaaatg gcaacattta cgggaagggg cttgtatccg acgaaaagtt tgacagctgg
    25441 gaggttaaag aaccctatga tggaactatt ctgcttgaat agcagatagc tggattaccc
    25501 catggttatt aggatttctc ctcaaatatt ctttcaccca gcattgttta tcctcgtcaa
    25561 tgccagtatc aagttgcctg ttgtacctat gtaatttttt tcccgggaat tacccagtag
    25621 aactcggaaa actaaacatt cggctgccat atttttcaag gaggcatact ggaacaattt
    25681 atcattcagt ttagactaga aaggcgatgg gtaattttgt ccaacatcgg gcaatgcttt
    25741 ttaagcttaa atttgtaact ttatgcagaa aaaaatacat agcatcctag aaactaagtt
    25801 ggtgaattat ctctttagct gtgaaaggat ttgcctacat acttgaaatg atttggctac
    25861 tgctcactgt tttcctagtt gcgctgggga tttatgaaac cgttcgaaac ggttttgcct
    25921 ccagctatat acttttttta ctagccctac tagccggcct tatgtatttt ataagacgaa
    25981 gacgaaggga aaagccggaa gagtaaacag gtaaaacatg ggtaccacaa taatcatcat
    26041 actcatttca ctttttttct cattcttttt tgctggaatg gaaatagcct acctttcggc
    26101 caataagctt aagctggagc tcgataaaaa aaaaggtgga tttacatcgc acgttatagc
    26161 ggttttagca ggtaataagg aacagtacat tgccaccatg ctggttggca acaacattgc
    26221 gctggttatc tacggtattg aaatcgccgt tctgcttacc cctgcattgg gtaagttgat
    26281 tcagattcct tcccttttac tcctggtgca aaccctgata agcaccataa tagttttggt
    26341 taccgcagag tttctaccca agtcaatttt taggttgcac cctaacgcct tccttaggtt
    26401 ttttgctttg cctgtttaca tcctgtacat tattttttac cccatcagca agttcacttc
    26461 ctggctttcc tactggatta ttaggttggt atttaggata aaggtaaagc acgatggctc
    26521 taagcctatc tttaacaagg ttgacctcga ttttctggtt gaccaggcct catcggccga
    26581 cgatggggaa ggagaccaca cccaggagct gaaaatattt caaaacgcac tcgatttttc
    26641 cgaagtacgt ctccgcgatt gtatgatacc cagaaccgat attgaagcca ttgatgtgaa
    26701 tgccactgtt gatgagctca gggccaagtt tgccgaaacc aactactccc ggttacccgt
    26761 ttacagcaac tcaattgata atattgtcgg ctacgttaat tctaaagttc tcttcattaa
    26821 tccccaaagc ataaaatcaa agctcatcaa catcgacttt gtccccgaaa ccatgccggc
    26881 caataaaatg ctaactcgtt ttataaagga gcataaaagt attgccattg ttgttgatga
    26941 atttggcgga acagctggcc tggttactat tgaggatatc atggaggaaa tattcggtga
    27001 aattgatgat gagcacgacc gcaacgagtt tgtcgagaag aagcttagcg acaacgagta
    27061 tattttttca ggacggcttg aggtgaaaca tctcaacgaa aactaccagc ttgatattcc
    27121 tgagtccgac gattacgaaa cacttgccgg ctttatcctg tttaagcacc agaacatacc
    27181 caaactaaac gaaaccatcg agtttgaggg ttttatcatt aaaatcctca gggtaaacga
    27241 aacccgcatt gagctggtga ggttgacaag gataaagggt aaagggtaag atagttcaag
    27301 aaattgcccg cctaccggcg gatttcagat tccagatttc agattccaga ttcaagaact
    27361 tgtccgccta ccggcgatta aagatttcag aactagtccg cctcctggag gatttcaaaa
    27421 cttatccgct acttgacaaa ttcaagaaga tctggcgctg gctaatttag gacaactgct
    27481 ggactaaaag cgtgtttttt cccaaacctg caggtttttc atctgtttac cttcaatatc
    27541 cagcttaact accgttttaa tcttgtgaaa tccattgttg ccggctgcgc ccgggttaat
    27601 aaacaacaag ttatgcttgc tatcaaactt taccaggagt atatggctat gcccacaaac
    27661 cagcaagttg acctcattgc tattaatggc ttcatacaca attggctcat atcttcccgg
    27721 cctgcctcca atatgggtca taaacacaac aatatcttcg acccgaaaat gctgtgaaag
    27781 tgggtagctc agcctaactc tggcatcgtc aacattgccg taaactgccc tgaatggcct
    27841 aaagctggcc aacctatcgg ccaagctaag ggagccaata tcccccgcat gccaaatttc
    27901 atcgacatcg gagaaaaatt ccattactcc atcatctaaa gtcccatgcg tatcggagag
    27961 tattcctatc cttttcatct gcctatccag ctaatattca aacttctact aagttgccta
    28021 agtattatgc tactagaaaa ctactattcc gacactcctg tatgcttaag ccgccatcca
    28081 ccggtcttcc ataaccactc attttgtgtt tattaaataa atgttggtta aaattcttaa
    28141 gcaatccacc gcaaatgaaa aattagcggc aacaggagtt actctaaaat cgaaatatat
    28201 aggttttgac ttggtattca gcttcaaggg gaaaaaaatt gccagtagta tttataacat
    28261 tcagactaat aggaaggtaa cagattatat gttttttttg ataaaaaact tgacacgagt
    28321 tgtttaacgc cataaaattg cctaccaaat agcttaatat ttaagcaggt tcgaatcaga
    28381 aatatttaaa atggttgttt ctatgagaaa attgagcggc ttcctctcat ttttttttat
    28441 tattttactg gtattacccg caagcaaaat agcagcacag aacataaaaa taagtggaac
    28501 agtaatttca ctaacaggag gatatcccat aaggggggtt acagtggtag tagttggatc
    28561 ggcacgggga acatttaccg atagcctggg ccggtacgag ctggtaaccc catcaatctc
    28621 caaaactcta cgctttacat gcattggcta taagacccag gaggttgaca tagccggacg
    28681 aagggttata gacgttgcgc ttgaggaaga ggccattgaa atgcaggagg taaaagttac
    28741 agcgttggga ataatgcggg cggagcgatc aaccggctat gctttacaaa gcgtaaaggg
    28801 tgaagacatt aaccgcacca gcgagacaag cctgctgaat gctcttcagg ggaaagtagc
    28861 aggcgcaatt attacaagca cgtccggggc agtaggcgca tcatccagaa tagtgcttag
    28921 gggaattaac tcgctcaata ccgataacca gcctctattt gtggttgata acgtggtgct
    28981 gagcaacgcc aatttcggaa acactacaac cgaaggggtg aacaggggaa gtggcgttgc
    29041 cgatattaat cctaatgata ttgaggatat taccgttcta aaagggccta atgcggcagc
    29101 actctacgga tcgcgtgcag ccaatggggt tataatcatt aagaccaaga gtgccgatgt
    29161 tgacgatcat tttggcgtat cgttcaacag caccatcacc atggagtcgc caatgcggct
    29221 gccagaattt caaaataagt acggacaagg ctctggtggt ttgtttgaat tttatgatgg
    29281 catgggtaat ggcattaacg acaatgttga tgaaagctgg ggaccgcctc tcgacattgg
    29341 gctaatgata cctcaatgga actctcctgt ggcaggcggg attaggcaac ctactccttg
    29401 gatttcccat cccgacaatg taaagaattt ttttgaaaca ggaatgactt ttaccaacaa
    29461 tgtttcagtt gaaggtgctg atgaaagcta tgctgcacgc ttctcctaca ctaataccga
    29521 gcaaaaagga atggtcccga acaccgacca gtcgaagaat aacgtaatgc tcaacgcctc
    29581 catttcgccc tatacctacc taaccctaaa tgccagcctt ggctacgtga acacccacag
    29641 cgacaacctg cccgcctacg gctacgatgc acaaaacgtt atgcagcaat ttctttggtt
    29701 tggccgccag gttgatgttg ccgatttgag gcagtataga tttcccgacg ggtcaaagcg
    29761 aaattggaac tacagctacc ataataaccc ctacttcacc ctgtatgaaa acctcaacgg
    29821 gcttgaccgc gacaggctgt ttggacaggg aagtataaac ctcaaattta ccgattggct
    29881 caaccttaaa gttggtgcag gcattgacta ctttaaaaac tacaacaacg cccgctccgc
    29941 ttttggagat atagacagcc cgttgggcta ctatggcgaa tcgcatctga ccttcaggga
    30001 gttgaatacc gactttctgc tctccttcaa ccgcaacctt tcaagcaagc tatcgttaag
    30061 ctttaacctt ggaggtaacc tgatgaggca atactcccaa tcgctcaatt cctacgccac
    30121 cgagctatcg gttgaagatg tttacacgct tgagaatagc cggggtccgg tggtttcaat
    30181 taactcacta tcccgaaagc aaattaatag cctgttctac aacggacagc tggtttggaa
    30241 aaatgccctg tttttcgact tcaccgggcg taacgattgg tccagcacgc tgccactgaa
    30301 caacaatacc tatttctacc catcgttcaa cctttcagct gtcctatccg atcttttcga
    30361 ttttcaatca cctactttga atatggttca ggtcagagct gggtgggcac aggtaggcac
    30421 tgatacagat ccctaccagc tacaaccggt ttaccgcttc gaccagggat ggcacgagtc
    30481 aaccaagctg cctgctattt acattcccaa cgatctacca aaccccaacc ttaagcccca
    30541 gcgtactaac tctcttgagg taggtgctac actgaaattg ttcatgagca ggcttggtat
    30601 agaccttacc tactacaacc aaaagacatt cgaccaaata atccgcctac ccgttagcgc
    30661 caccaccggc tacacatcca aaattgttaa cgcgggtgaa atagacaaca gcggtattga
    30721 gcttacgctc gatggcgtgc cggtgagaac acgttccgga ttcgaatgga gcataaacct
    30781 gaacttcgca tggaacaaaa acgaggtgaa ggagttagcc gagggactca agcagtacgt
    30841 tttgggtgga tactggagcc tcgacgtaat tgcaatccca ggtcaaccag ttggcacact
    30901 ctatggatac gactttaaac gtaatgaaaa cggaaaaata gtattctacg atgggttacc
    30961 tgcacagggc gaaatacatg ctttgggtaa ctacaccccc gactggacag gtagcattgc
    31021 taactcgcta cgatacaaaa atttatcact caactttctc attgatgccc gatttggtgg
    31081 cgagatttat tccatgactt caacctgggg tagatacgct ggtgttttaa aggaaacatt
    31141 aaaagggcgt gaaggtggaa ttataggggt tggcgtaatg cagaacgaag cgggtgaatg
    31201 ggtacccaac aatgtagtag ttacggccga agagttcaac aaggctgcct acaacaacaa
    31261 catcgcctac tccagcgttt ttgacgccag ctttgtgaag ctccgcgaga tatcgcttgg
    31321 ctacaccttc aaccacattg ccaataacct tattaaggag atttctatag ccgttgttgg
    31381 tcgaaacctt gcactgctct acgcccgagt gccccatatt gacccggaaa ccgctttcag
    31441 caacagcaat gtccaaggcc ttgaatttgg ccagctgccc tcgccccgta gctatgggtt
    31501 aagtttaaat ttaaagtttt aatgctgaca aataatggat acccctatgt ggagatggtt
    31561 taagatatat agatggattg ccataacggg catacttgta ggcatggtat catgcaccgg
    31621 tgattttgag gagctaaaca ggaatccaaa tgcgattggt gcaaatgctg accaccccga
    31681 catgctgctg acccatgctg tcgaatcgct gggcgatcag cttttcgatg tgtggctggg
    31741 tcacgaaatc ggctcatgct gggttcagca catggcaaag gttcagtaca ccgatgagga
    31801 caggtatgtt ccccgcaatg atgttatcaa ttccgtttgg aacaaccttt acgccgggcc
    31861 gggaattgaa attcaaagaa tttacgagat aggccagaga aaaggccatg ccaactacat
    31921 cggcgttgct ctggtgctga aatcatacct tttttcagta ctcacggatt tgtatggcga
    31981 tataccctat acccaagcat tcaacccaga tagcaccctt acgccacagt acgatacaca
    32041 ggaaagcatc tacctgaacc tcattcagaa cctgaaatat gccaacgagc ttcttaaccc
    32101 ttatggcgat ggtattgagg gcgatatcct ttttcatggc gatatcctta aatggaagaa
    32161 atttgccaac tcattgcgtt taaggatttt actccggatg tcgggtcatg ccgaacagga
    32221 gcagttcgtc aagtcagaaa tgtcctcaat tttgggaaat tctaccgaca atccaatatt
    32281 tacctctaat tctgacaatg cagcgcttca ctaccttggc tcttacccca acaaccaccc
    32341 cttgaacgaa acctacaaaa cccgcgatga ccacagggtg agcagaaccc ttgttgatgt
    32401 catgtataac ccaggtaacc atgataaccc cgactggaga attgtggttt acgcccaaac
    32461 gccacaggcc ggaggcttct ggattggcct tcccaacggt cttacatctg ctaaagctgc
    32521 agcataccta ggcaatggaa tcaggcgtac ttcaaaaatt ggggagtatt ttattcgacc
    32581 caacgcacct gccatgttca tgagcttcgc cgaagtaaac ttcatccttg ccgaagctgc
    32641 actacgcaac ctgattgaca tcagcccact taccccaaag gattactacg aaatgggaat
    32701 tgatgcttca tacaggcaat atgccaggga aatagttgaa aatatacccg tttactaccc
    32761 atcgtttgaa ggggttagca taaacataga cgattttatt accgatttcc tgaaccataa
    32821 cggtgcatgg gatgccgcta acccactaca aaaaatcgct acccaaaaat gggtggccat
    32881 gttcgaccag ggacttcaag cttggtttga atggcgaaga actgggtatc cggttttaac
    32941 gccagcagag gatggactga atggtggtaa aatacccgta aggcttcgct acccatccga
    33001 cgagtactcg cgaaatggtg aaaacgtggc aaaagcagtt gcactgcagg gagacgacaa
    33061 cctaaatacc ccggtttggt gggattgcaa ttaagctgat tttagaagat aacaatatct
    33121 aaaacttatg gaaaagttca aaatgtattt cgtgaagctg attttgccca ttgcccttac
    33181 taccttgtcg ttaggttcct gtattgaaaa ccaggacctc atgaccagca acgtgcgtac
    33241 aggaggtatc cttgatgcgt cagggttagt gcagtttaag cccagtgccg ctaaccagga
    33301 cgactc
//
LOCUS       DAOPHX010000008        32776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_1282_length_32776_cov_6.147948, whole genome shotgun sequence.
ACCESSION   DAOPHX010000008 DAOPHX010000000
VERSION     DAOPHX010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 32776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32776
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1282_length_32776_cov_6.147948"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(315..785)
                     /locus_tag="PK502_01360"
     CDS             complement(315..785)
                     /locus_tag="PK502_01360"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPG99496.1"
                     /translation="MRNPYQNQPSHGCFCCSSHNPIGLKLEFWFDDERGTVGTQWLPS
                     ENFQGYHDVLHGGIQSTLMDEVASWCIYILAGTAGVTSKMEVAYKKPVQISNGMVSVV
                     GRMEKMEKRLAIVKTELFDGKGILCSTGQITYFTYSAEVAKQQFGYPGLAEFLS"
     gene            917..1519
                     /locus_tag="PK502_01365"
     CDS             917..1519
                     /locus_tag="PK502_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPG99497.1"
                     /translation="MHGNATKMKLMETARELFSAKGVDRVGVREIAAKAGVNLSLMNY
                     YFESKENLLEQIFEFSVRENGHKLRQILSSGLPLDEKIRQYVYMYIDILIDDPLLVPF
                     VLSTIHRKADKSPYLKSIHTLYSTESFSKQLKEEADKGHIRPIDTEQFFVSMISLIIF
                     PFAVEPLVKYRMSLNNDQMNIFLAARKEHVFDMLMAVLKA"
     gene            complement(1644..3011)
                     /locus_tag="PK502_01370"
     CDS             complement(1644..3011)
                     /locus_tag="PK502_01370"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG99498.1"
                     /translation="MDYDVVVIGAGPAGYVAAIRAGQVGLKTAIVEKKLIGGMCLNWG
                     CIPSKAIIESGRLYRRIKDDARFFGVDGIDEQNITFSWEQARKRALQVAKQLTSGVEL
                     MLKKNGVEIINGEARITSENSVTVANRNITASNIIIATGSHHLPLKAKLPSEKVVELE
                     QFYSLKELPEHIAVVGEGSIAIELAQFFNLIDRNVTILVPGERMMPLADEYLAGWMLS
                     KLKRDGIRVIFNANDMADSSHFEGNQLVVGKDKFPCDIIINSKLRQGIIPPSDIPLET
                     ANGFIATNDFFQTSFPNIFAVGDVNGEMMFANAASTQGFHTINFIKGIKEPFDKTRYP
                     LNMYSSPEVAQIGKIESQLKADVVNYRVSELPLSANGKALSEGNTEGFVRILSEVKYG
                     QVLGVQIIASNATDMISEAAAFMEAEATIYDVAKTVHAHPTISEVFMEAGFVAVDSAK
                     EENRQ"
     gene            complement(3028..4314)
                     /locus_tag="PK502_01375"
     CDS             complement(3028..4314)
                     /locus_tag="PK502_01375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HPG99499.1"
                     /translation="MRYIFNFPDIGEGLDEGTIAEWYVQKGQHVEAGDPLVNMETDKV
                     VTDIPSPKSGTIVATYGKIGETIRVGSPLVEIDIEGVEGEAAVAEVRKEEPVEEGAGV
                     VGTLEVAGNSAFLPAGDEGFEQQPAADSKIGRKALATPVARAMAKELGIDINRVTGTG
                     PSGRVTTQDIENFLKKGGTQKSLSVEPSATMVASGEQLVEYEPLTQIRKTIARNMIQS
                     KQNAAHMSVFDEVEVSELVRIRAKYKDAFAEKGVKLSYLPFILKALAVALSHHKTLNS
                     QLDLENNRIIYKKYFNIGIAVDTEEGLVVPVIRGVDKLSVFEIAERVNEIAEKARNRK
                     LTLDDMKDGTFTVTNYGSIGGQFAVPVINYPQVGILGIGRLVEKPVISHGELAKGMVL
                     PLSLSVDHRIVDGGEVARFLNRLIAYLADPVSLIME"
     gene            complement(4341..5318)
                     /locus_tag="PK502_01380"
     CDS             complement(4341..5318)
                     /locus_tag="PK502_01380"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HPG99500.1"
                     /translation="MKVMTMVQAINNALDIKLQEDPMIVTYGEDVGVEGGVFRATEGL
                     QKKHGTNRVFDSPLAESAIVGTAVGMAANGLKPIVELQFCGFVYPSYNQIISHVARFH
                     NRSRGRYKMPMVIRFPYGGGINALEHHSESMETIYGHIPGVKVVIPSTPYDAKGLLIS
                     AIESEDPIIFMEPKRIYRAIKQEVPEEKYSIPLGKAKVLQQGTDITLIAWGAMVREAQ
                     KAIVMAKKDGIAVELIDLRSIYPIDRQTIVESVRKTGRVVIAHEEPLTFGTGAELTAI
                     INEEAFLSLEAPVNRVAGFDTIVPLPRGEHHYIISPEKIYYNILKTMKF"
     gene            complement(5448..6536)
                     /gene="pdhA"
                     /locus_tag="PK502_01385"
     CDS             complement(5448..6536)
                     /gene="pdhA"
                     /locus_tag="PK502_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HPG99501.1"
                     /translation="MLSDFSPLEDRMFQVMDNDGRVINPKYKPNIEPERLVKAYKDML
                     FARQADLMAVSYQRQGRMYTYPPNFGQEAISGGLAVVMREQDWLVPAFRELGAWLAKG
                     ATLKDVFLYFIGYEDGTVFPNAQNILPISVPIASQLQHAAGIGYAINYKKEDSVVYAI
                     VGDGGTSEGDFSEAVNFASVWKAPVVFIVQNNQYAISVPFKMQTRSVNIAVKSYAFGI
                     PGIKVDGNDLLAMTSALQEASAHARAGNGPVLIEAFTYRRGSHTTSDDPTKYRSKEEE
                     EAMAATDPIDRLRKYLIGQKLYDASSEGELISQFKQEVERQFGEAENYHPYPLEDVFK
                     YTYVDMPDELKQQKIEHEKFLKWKESLK"
     gene            6793..7764
                     /locus_tag="PK502_01390"
     CDS             6793..7764
                     /locus_tag="PK502_01390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004351088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG99502.1"
                     /translation="MKYSVVIPVYNRPLEVKELMESLTEQAFKDFEVLVVEDGSSIPC
                     KQEVLEYKGRLNLTYLEKENTGPGLTRNYGACSANGEYLIFFDSDCLIPNGYFQEVEK
                     ELASAPADFFGGPDRAHPSFTPIQKAISYSMTSFLTTGGIRGGKHKLDKFLPRSFNMG
                     VTAEAFRKVEGFSAMRYGEDLDLSLRLVAAGYSSRLFPGAFVYHKRRTDFRKFYRQVF
                     SSGFARINLHVRHHGSMKLVHLLPSAFVVGICLLLVASIFFPWLLMLPLLYCFALFTD
                     SLYKGNSPWVALLCIPASWVQLFGYGLGFIAACWKLTMRGKNYFLPD"
     gene            8171..8680
                     /locus_tag="PK502_01395"
     CDS             8171..8680
                     /locus_tag="PK502_01395"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HPG99503.1"
                     /translation="MHQGNIIIAYKGSVNADIIADTLSLVEVKLNDEMEHSLTRKKVY
                     NVLVECLQNLFHHVDTVPGFYNEDNFGVFILSKIDEGFRVSTGNMVNGQKKEILKQKI
                     DKINSLDKEELKEFYKFVLNNQMFSDKGGGGLGLIDIARKTGNKLEYTFTPFDESFDF
                     FTLSATIND"
     gene            8699..9079
                     /locus_tag="PK502_01400"
     CDS             8699..9079
                     /locus_tag="PK502_01400"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPG99504.1"
                     /translation="MELLSIEGTPKTPTVTMNPNTGVIEIKGRSIPENSIEFYKTIID
                     WIDEYSKSPQPNTVVNIQFEYFNTSSSKCILDVFKKFEMLKKNKADVVINWYYEEDDE
                     DMLEAGEDYESIIKVPFKMIQIVD"
     gene            9261..10274
                     /gene="murB"
                     /locus_tag="PK502_01405"
     CDS             9261..10274
                     /gene="murB"
                     /locus_tag="PK502_01405"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPG99505.1"
                     /translation="MPVIKKNVSLKPYNTFGVDVSASYFVKANTLEKILYAINFASYN
                     QLPIFIIGGGSNMLLTKNLNGVMVNPFIQGLTLTEETDSDAIVKVGAGMTWDALVNIA
                     VDHGWGGIENLSNIPGSVGAAPIQNIGAYGVEAKDAIFKVEAIEIKTRKSLEFSNSEC
                     RFGYRDSIFKQELKGKVIVTHVWFKLSKKPEYQLAYGNLKEEVEKLGGVSLKNIRQAV
                     INIRSAKLPDPAVIGNAGSFFKNPVVEDVLFQKIQQDYPDVPHFSVSESHVKIPAGWL
                     IEQCGWKGKRVGSCGVHDKQALVLVNYGGATGEEILTLARSIQQSVYDRFHINLDMEV
                     NIC"
     gene            complement(10313..12094)
                     /locus_tag="PK502_01410"
     CDS             complement(10313..12094)
                     /locus_tag="PK502_01410"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HPG99506.1"
                     /translation="MDTKINERLQLLRRQMKSRGFDAYVIHHSDPHISEYIPDYWKVR
                     EWASGFSGSAGTLVLTEKKAALWTDSRYFLQAEQQLAGTGIELCKQGLPDTPDIPGWI
                     KLNLEAGSTVGCNGGIISVSAARYLSKSLEQLGFKCSFDDDIASTVWLDRPPLPTAKA
                     YIHAAEFAGISAGDKLESIRRNLLNINATACLMCSPDEVCWTLNLRGSDIQFNPVVLC
                     YAYVDLERAILFVDGRKLDDEISNFLTSAGVSIMGYEQVGNFISNISRHTVLVVDSDK
                     TNYKLYNQIPQTVKIVEKIGIVTEIKAKKQKPEVEGIHQAMVQDGVALVNFFYWLEEV
                     LGRERLTELTVAKKLLDFRSKQQHFVSESFETIAGYRDHGAIVHYSATPESDYTIEQD
                     GFLLIDSGGQYLNGTTDITRTVHLGKPNPDEMKHYTLVLKGMISLAKARFPFGTRGSQ
                     LDTLAKMALWSNHLTYGHGTGHGVGYFLNVHEGPQQIRPENHLPIEPGMVMSDEPGIY
                     ISGCYGIRIENLILCTDDISNDFGRFLTFETLTLCPIDTKPIAVSMLSDDERDWLNHY
                     HATVYEKLSPYLDEEHKTWLKKKTLPL"
     gene            complement(12138..14969)
                     /locus_tag="PK502_01415"
     CDS             complement(12138..14969)
                     /locus_tag="PK502_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPG99507.1"
                     /translation="MNGNSLSSKESETHIYLQHIHRYLRAGFWLWNSETNHLLLGHKF
                     QEALGVKADELGIHLDDFFSVVHPDDRRQLSTILFRLLKQKLRYREFDVRVMVNDVWH
                     RLRIHALVGEIATDGSVQSLFGKFLPADPRQPISGPAIPASTFMGFDAFTYNPEEGQL
                     QWQFQPSSMFWGNTPKGKTWNFSEFRAILSPSSFKELSQSWQVFANGNEEFFTRNLTL
                     SSPQNAKRIITLLARKVEGSRYIQGVMIDTSKLCGIADKSSFLHFYSSIASSHRLLMI
                     ELSREGAIKGCNRITEKSFGYSLAELQNPQLLPSLFTNADSFKQLVTYANSGGSKPFV
                     CRVRNREGQVKDISWILLKLPEVVGMEGNTLIGQDVTRQVQVNFEVNLLQQRYQAIKL
                     ANAELQGAANFEGVFSCFGQQLERIFPSMVTVVLSYNAADGFITVEGIFGIQHKTWES
                     LIFDLGWNPVGRRFLLHDKDKHAIKSAAAYRVNESFTGIMEGYISMSASKIIERYINI
                     EAIYAEGLVSGNHMYGGVLLLKPEPADEPDLTLLEELASVAVKTIQRIDTAEGLQQQI
                     FTLKSKIEQKDELFSQLSHQLRTPLNAVLGFSQLLLELNQPDDLLKKYIGIINSKGKQ
                     LLRLVNDTIDYNLIEKGNLTLVKSTFNLNHLLQQLHRSFSQQFELYQNEGIKLKFIQP
                     GNAENIFLKTDEGRLEQALANLLDGIFKTVERGTIELSCRKENSKVMIFLRISGAGAE
                     SKVNESLLDQFRQSENIESPMQSLLGIRMARQIVTLLGGEMRFDIGSNDNAEVEVLLP
                     LTDSSTSGSNNNIEDNAPVYPDFKNKVILIVEDEEVNYLLFRELLVSCGATTLWAKNG
                     REAVNLVSTINQTIHLVVMDIQLPVMDGYAATMEIKQINPKIPVIAETAYISADDRKK
                     AEAAGCDGYITKPIEPKVFYEVLEIFLGT"
     gene            complement(15101..17602)
                     /locus_tag="PK502_01420"
     CDS             complement(15101..17602)
                     /locus_tag="PK502_01420"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPG99508.1"
                     /translation="MPEKQDKTKVTAAFYFFKAGLLLLLMVTVMVVKGQTGQLLVVGF
                     VTDSVGSPVPFASVVVEGGPIGVSTDINGRFRITLPAAQSAVLTISCVGYKSTSVLIN
                     ADVNSPIVELRVKLDVNELLIDEIRVSSDRRWTGNIERVNARDISFMPNVSGNFESII
                     KLMPGVASTNELSSQYSVRGGNFDENLVYVNDIEIYRPFLVRSGQQEGLSFINSDLVS
                     AVEFSAGAFNAEFGDRMSSVLNVSYRMPTSYQSGVSLSLLGASAYTEGVLGNRKFSYI
                     TGVRYKTSSYLLNTLDVKGDYTPSFLDWQGLFSYRLNDKLAFSFLGNYATNSYQFAPD
                     VRETRFGVFSNALQLKIYYEGKELDRYISGMGAAYVEYAPSQNTSIKIYSANYLTRES
                     ETFDLLGEYFLNELDNSMGSGSYGDSLINVGIGGFLNHARNFLFARINNFGLVGSVRF
                     SNAKLKWGAQLQHEFFDDELNEWNLLDSSGYAIPYNGSAIGLSNVMVAGNSTSVNKLS
                     AFSQLDYSLSRGAFTLATNTGLRLLYWDYTGDVLVSPRVSLSLKNQRLKNFNFHLAGG
                     VYYQPPFYRELRMPNGSLNPNIKAQRSVQLLAGLEYYFTAWNRPFKLSVELYNKWLDN
                     LIPYKVDNVRIRYAGQNLAKGFARGVDIKLNGELLEDAESWVSISLLKTMEDILHDSY
                     VDANGQTVYPGYYPRPTDQTLGFNLFFQDYLPGNPTFRVHLSANYGTGLPVGVNGIDR
                     YDVTFRMKDYKRVDIGFTKILRDEISSNGISRKLKGFKGIWLGAEVFNLFNFNNTVSY
                     LWIQTVGNQQGESDTFAVPNYLTSRRLNIRLQLKF"
     gene            17811..18218
                     /locus_tag="PK502_01425"
     CDS             17811..18218
                     /locus_tag="PK502_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPG99509.1"
                     /translation="MIQQVEFALPKFPIGFHLITELVVKKLPALPETGMANIFIKHTS
                     AALTINENADYTVRKDFQKFISTLVPFSNTYEHTLEGEDDMTAHIKSSLLGQSVWIPI
                     SNGKLNLGTWQGIYLCEFRNHGGNRKLVITIIS"
     gene            18517..20127
                     /locus_tag="PK502_01430"
     CDS             18517..20127
                     /locus_tag="PK502_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPG99510.1"
                     /translation="MEKLLLLGDEAIAQGAIDGGLSGIYAYPGTPSTEIMEYVQGSKM
                     AAERNIHRQWSANEKTAMEAAIGMSYAGKRAMVCFKHVGLNVAADAFINSAITGVNGG
                     LVVVSADDPSMHSSQNEQDSRFYGKFAMIPILEPSNQQEAYDMAYQSFELSERLNVPV
                     LFRITTRMAHSRADISRHEVKDQNKLRMPLDPRQYVLLPAIARKNYKKLLSTQPAIEL
                     EAEQSKFNAFIPGENHDMGIVATGIAYNYLVENYPNHQCPFPVVRIGHYPIPRKKITE
                     LYESCKSLLVIEEGYPMVEEMLRGFFNVGKPIHGRLDGTLPRDGELNPNIVAKALGFP
                     AAGEEPLAEVVKNRPPSLCMGCGHRDVYTDLNEAIKDYSAGRVFSDIGCYTLGALPPF
                     NAINSCVDMGASITMAKGAADAGLVPAVAVIGDSTFTHSGMTGLLDAVNENSPITIII
                     SDNSTAGMTGGQDSSATGKIVDICRGMGVDPSHIRVVVPLKKNHEENVKIIREELAYN
                     GVSVIIPQRECIQTFARRKKTEKKESND"
     gene            20237..20818
                     /locus_tag="PK502_01435"
     CDS             20237..20818
                     /locus_tag="PK502_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPG99511.1"
                     /translation="MNNDIILAGVGGQGILSIAAAIGLAAVNSNLHIKQSEVHGMSQR
                     GGDVQSHLRISDAPIASDLIPAGKAHLIISVEPMEGLRYMPFLSKNGWLVTNITPFDN
                     IPDYPKVDEILKRVKAVKNHIALDADSIAKECGSTKASNIVMLGAASPFIDIPAAGIE
                     EGIRQLFNRKGEDIVNLNLKAFRAGREYSEKNR"
     gene            21074..23713
                     /locus_tag="PK502_01440"
     CDS             21074..23713
                     /locus_tag="PK502_01440"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPG99512.1"
                     /translation="MYKLLPPFCIAILLFAVQPAQAFLDEDKKICQAVRVNPKPPVVD
                     GSLDDEVWEKGEWNGNFIQHEPYNGNEPSQKSYFKILYDSDYLYAAFKMSDSEPDKVV
                     SRITRRDDADGDRVTLCIDSYFDQRTAFLFTTNAAGVKSDEVMTENGDKEDMNWDPIW
                     WAKAVKTPDGWTAEMKIPLNQLRFANSSDMKWGVQVMRYIYRNQETDLWQHVPKDAPG
                     FVHLFGELHGLNGLAPKRQVEVAPYMVTKTERLEAESGNPFMTGKNNGFNAGVDAKIG
                     ITNNFTLDLTVNPDFGQVEADPAVVNLSAFETYFEEKRPFFIEGSGMMNFSLMFGDGD
                     LATQNLFYSRRIGRAPQLQPDLDDNEYAKIPSNTAILGAAKVTGRTGKGMSVGILETI
                     TNSTYATIDSMGVRTHEIAEPLTNYTVASVRQEFSGGNTIISGIATSTNRQLNDLSSD
                     YFHQNAFTGGMDLQRFWSNKKYLFCGKLYFSHVNGSKEAIARTQTSPTRYFQRPDNNY
                     ARLDSTRTSLTGTGGLLQFGRVGEGHLQYMGAVTWHSPQLEINDIGYIQNVDDIFQVM
                     WVGLRFWKPTLWFRYVNFNLNQWATWNFGGQSTDKGGNINFNLQFTNYWTFNAGINYN
                     SESLSAHALRGGPMLKIPGNWNAWYNIGTDGRKKLVFYMGGSTLESNENYAYYQSVYA
                     TLSYRPLKNLQLSLNPSYSVNSDKLEFVENVDYGNETRYIQGKLSQKTFALSLRVEFS
                     INPDLSVQYYGRPYLSSGKYTKFKRITNPMANSFDQRFVTFEPSQLVYNQDEEEYYVD
                     ENNDGGSDYSFSNRNFNFMDLQSNLIVRWEYRPGSTLFLVWTLGKQAYESEYGSSLNN
                     NIETLYDAHPHNIFLLKFSYRFN"
     gene            24034..24582
                     /locus_tag="PK502_01445"
     CDS             24034..24582
                     /locus_tag="PK502_01445"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPG99513.1"
                     /translation="MKIYTKTGDKGMTSLIGGMRVPKYHPRIDTYGTVDELIAFIGLL
                     RDQPVDGHTRDMLIFVQDKLMSCAAVLAAEEPNSKTDLPTVSDADVRKLEADIDAMDA
                     LLEPLTSFVLPGGHPTVSLCHVCRTICRRAERLAVKLATETPLPDNLTIFLNRLSDYL
                     FTLSRKLAKDLKVDQIKWHPDK"
     gene            24668..24889
                     /locus_tag="PK502_01450"
     CDS             24668..24889
                     /locus_tag="PK502_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HPG99514.1"
                     /translation="MYWTLELASKLEDAPWPATKEELIDYAIRSGAPLEVIENLQELE
                     EETEIYESIEDIWPDYPTKEDFFFNEDEY"
     gene            complement(25110..28211)
                     /locus_tag="PK502_01455"
     CDS             complement(25110..28211)
                     /locus_tag="PK502_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG99515.1"
                     /translation="MSESILKALMQLFAIISRPVAEEEMEANDYSDRRRVVEEFLRNQ
                     LNQELVAEYIKVFDNYYNLYQEKQSEKTKSKKRTSSSSVRVLKICAQINEELQQQQKI
                     VVLVRLLEFVKPEEGKVTEQEMAFISTVAETFYVSADEFNMLSDFVLSSFNQIPITPN
                     VLLIDGNKDFELPGVKHIYAEGLLDELRVVFIESANIFFLRYRGTHELYLNGHLIQNE
                     KIFVLSNGSSIRNPKIKPVYFSDIVGRFALDKLKERIVFEALDVEYRFRSGKIGIHQM
                     TFTEESGHLVGIMGASGAGKSTLLNVLNGSEKPTAGNVLINGIDIHRQPELVEGQIGF
                     VSQDDLLIEELTVYQNLYFNARLCFDNYTPQQLDEAVSRTLVNLGLNEIRDMRVGTPL
                     NKKISGGQRKRLNIALELIREPAILFLDEPTSGLSSRDSENILDLLKELTLKGKLVFV
                     VIHQPSSEIFKMLDKLLILDTGGYLIYNGDPIESIMYFKSRVQHANWNESECATCGNV
                     NPEQIFNIVEANVLDEYGNPTRARKISPVEWNNHYKKWFSAQPQLDGIPDFLPKIHFK
                     TPGWFKQLKIFVKRDVLSKLANTQYMIINVLETPLLAFLLSYIIKYFNIDVSNRVGYT
                     FMENSNIPVYLFMSVIVGVFVGLTVSAEEIIKDRKIQKRESFLNLSWSSYLMSKVAIL
                     LVLSAVQAFMFVIVGNSILEVRGMYFEYWLVLFSAWFSSNMLGLVISDSFKTVVTIYI
                     LIPFLVIPQIILSGVIVKYEKLNPKISSPSSIPFYGELIVARWAYEALAVEQFCNNPY
                     EKPFYVFDEAMSISDYKRNYWLKSLQNKVDFCSRNLGLPEKSEEVEDALTLLRNEIMH
                     EMTSPQGSKLTFSKYNQVSMAGVSPELLDELKSYLELVRKYYVKLYNKASSQKDAYIS
                     SFQAAPGGQEAFVNLKRQYYNESLTDFVRNTGEVERITEYKGRLIQKIDPIFLYPDTK
                     FVKAHFYSPVKPVFGKFAGTFWVNVAIIWISNILLYLILYYRLLKRFLEQFDRIGAEK
                     EAN"
     gene            complement(28234..28650)
                     /locus_tag="PK502_01460"
     CDS             complement(28234..28650)
                     /locus_tag="PK502_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99516.1"
                     /translation="MAKKILIPFILIGLFSVKATAQVENLRNTCALNFPPEYVSDGQE
                     YFASLKPDQVIEFRTTFFGENTYRITTCTSVKKGQVVLTVYDTDKNLLFSNRDYDYSP
                     YWNFRFTSTVICIIQVDVVSQQFTPGYVMLLIGFKQ"
     gene            complement(28663..29562)
                     /locus_tag="PK502_01465"
     CDS             complement(28663..29562)
                     /locus_tag="PK502_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99517.1"
                     /translation="MLNFKRIVFFVSGCFLIFGLTECNHPNKSDNQTIKEDVSIDTLP
                     TLIKYESSIFPIPSPWQVVGLVKNLSIPYNENLLNRYDRYQKYGKSFKQALNLGIYGA
                     DISYLCIYDRTQEAIKYLDAIRKLCDQLGLEQIFDQNFFDAVEQNINNKDSLARIITK
                     AYSSSDLYLKTNERSDIGALIVAGSWIESMYFLTQIALGNSNRDIINRFGEQKHPLDN
                     LIELLTPYYYKSSDFSELIDELINVAYEYDGIIYSYKYKEPIIDAANRQITINSTTQV
                     VVSEYHIRIISSKIESIRNKLVE"
     gene            29815..30696
                     /locus_tag="PK502_01470"
     CDS             29815..30696
                     /locus_tag="PK502_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HPG99518.1"
                     /translation="MKKVTLLTAAFMLSLTSVAQQKVLTLEQAVLRQYADFRPQTLAN
                     FSWKANVNAYTYTEGDSIIEVSVPSGMPKTVMTLELLNRLLSADSIPALKRLAYTWAS
                     ENVIKVFSAPYLAFVDVGLGKITRRFTIPEEAENLELSPNNTSLAYTVANNLYITDAK
                     GTPVAVTTNDNPGIVNGQAVHRNEFGINKGIFWSPSSSYLAYYRMDETMVTDYPLVDI
                     TQRIARVEPVKYPMAGMKSHEVTLRVYNLSTHSNIEIKTGLPAEQYLTCVTWSPEKVD
                     FYCNAEQGTEPHAAQPV"
     gene            30677..31948
                     /locus_tag="PK502_01475"
     CDS             30677..31948
                     /locus_tag="PK502_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPG99519.1"
                     /translation="MRLNRYSAQTGELMATLFEETDSEYVEPQQPLAFLDDNSGRFIW
                     QSRRDGWNHLYLYDVNGKLLRQLTRGEWEVTDINSVDLPNGKIYITATRESPIERQVY
                     AVDLKKDNITMLTQGAGFHNALFSPDCKYFVDFYSSISIPNRVNLCTGKGKIVKNLVN
                     APNPLASYNVSLPELLTLKADDGTTLYGRMIRPVNFDSTLRYPVVVYVYGGPHSQLVR
                     NSWMGGASLWECYMASRGYILFTLDNRGTSYRGSNFEQTIHRRLGTIEIADQLAGVNF
                     LKSLPYVDSTRIGVHGWSFGGFMTISLMLRAPEFFKVGVAGGPVTDWKFYEVMYGERY
                     MDTPQENPDGYSTSDLKQYVKNLKGKLLIIHDDMDKTVVPQNSLTLLHEFVKSNVQVD
                     FFLYPQHEHNVMGKDRVHLIDKIIRYFDDNL"
     gene            complement(32277..32519)
                     /locus_tag="PK502_01480"
     CDS             complement(32277..32519)
                     /locus_tag="PK502_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99520.1"
                     /translation="MELKDYFKENTPDSIISEFEGHSSYFFHIIYNETGIAENQLFDN
                     SFEKNIMNNNWLKGNKITFYYKDNKFIKTEPYFSNE"
BASE COUNT         9157 a         7684 c         7097 g         8838 t
ORIGIN      
        1 gtttcccgcc tttccgggca ctgcggagaa taccttttcc cccgttgggg ctgaaatcaa
       61 gggtaaaggt taatacaagt ataaaggtta aaggataaag gataaaaaaa ttcaagcaag
      121 attcaagaac ttgtccggct cctgacggat tcacccttcg acaggctcag ggttcaaacc
      181 tgtccgccta ctggcggatt taagaacctg tccggctttt gacggaaggt tgagtggttc
      241 tgacggttat attgttcaat ttctatttat caactttcaa cctctaccct gtttcctgta
      301 tcccttccct acacctagct tagaaattca gccagtccag gataaccaaa ttgctgctta
      361 gctacttcgg ctgaataggt aaagtaggtt atttgaccgg ttgagcaaag aatgccttta
      421 ccatcgaata gctctgtttt aactattgcc aaacgtttct ccattttttc catcctaccc
      481 acaacgctaa ccatgccatt cgagatttga actggttttt tataggcaac ctccatcttt
      541 gaagttactc ctgcagtccc ggctagtatg tatatgcacc agctggcaac ctcgtccatc
      601 agggttgact ggattccgcc atgaagcaca tcatggtatc cctgaaaatt ctcagaagga
      661 agccactgtg tccctacagt tccccgttca tcgtcaaacc aaaattccaa cttcaaccca
      721 atggggttgt gggacgagca gcagaagcat ccatgactag gctggttctg gtatggattt
      781 ctcaacttta taaccatgtt tatcgagtag tattattgca cacaatttta gcattatttc
      841 acaaaacttt aagcctagta gctctttgtt ttttagcgaa agagttaaat ttggtaaacc
      901 tataaatctg gactctatgc atgggaatgc cacaaaaatg aagctgatgg aaaccgcccg
      961 cgagctattt agcgcaaagg gcgtagatag agtcggagtt cgtgaaattg cagctaaagc
     1021 aggggttaac ctttcactca tgaactacta tttcgagagc aaggagaacc tgctagaaca
     1081 aattttcgaa ttttcggtta gggaaaatgg gcataagctt aggcagattc tgagctctgg
     1141 ccttccgctg gatgagaaaa taaggcaata cgtttacatg tatattgaca ttttaattga
     1201 tgaccctctt ctggttccct ttgttctgtc aaccatccac agaaaggcag ataaatcgcc
     1261 ctacctaaaa tccattcaca ccctatacag caccgaaagt ttctccaagc agctaaagga
     1321 agaggcagat aagggacaca tcagacctat tgacaccgaa caattctttg tgagcatgat
     1381 ctccctaatt attttccctt ttgcagttga gcccttggtg aagtatagga tgagccttaa
     1441 caacgatcaa atgaatatct ttctggctgc ccgcaaggag catgttttcg acatgctgat
     1501 ggcagtgtta aaagcatagg ttgatgagag ccggcgctag ggtaatcgtg caaaacaaag
     1561 tggaagcatg gggcaatata gctgaaaagg ccacggctta cctagtatag gctccgtgac
     1621 ctcagcagtt catcctgaaa aggttactgt ctgttttcct ccttagcgct atcaaccgct
     1681 acaaatcctg cttccataaa aacctccgaa atggtgggat gggcatgaac ggtcttggcc
     1741 acgtcgtaga tggtagcctc ggcctccatg aatgcagcag cttccgatat catatcagtt
     1801 gcgttgcttg ctataatttg cactcctaaa acctgcccat attttacttc ggataggatt
     1861 cttacaaacc cttccgtatt gccctccgaa agggctttcc cattagcaga aagtggaagt
     1921 tcgctcaccc tgtagttcac cacatcggct tttagctggg attcaatctt gccaatctgc
     1981 gctacttcgg gtgaagaata catgttaagc gggtacctgg tcttatcaaa aggctccttt
     2041 attcctttga taaagttaat agtgtggaag ccttgagttg acgctgcatt ggcaaacatc
     2101 atttcaccat tcacatcgcc taccgcaaaa atgttgggaa agctggtttg aaagaagtca
     2161 ttggtagcaa tgaaaccatt tgccgtttca aggggaatgt ccgagggcgg aataatgccc
     2221 tgccgcagct tagagttgat tataatatca caggggaatt tatctttccc aacaaccagc
     2281 tggttccctt caaaatggct actatcggcc atatcgttgg cattaaaaat taccctgatt
     2341 ccatcccttt tcagcttgct gagcatccag ccggcaaggt actcatctgc aagcggcatc
     2401 atacgttcgc cgggaacgag aatggtaacg ttgcggtcaa tgaggttgaa gaattgagcc
     2461 agctcaatgg caatggatcc ttccccaacc acagctatat gctccgggag ctccttaaga
     2521 ctataaaatt gctccagctc aaccaccttt tctgatggga gcttggcctt taaaggcaga
     2581 tggtgcgagc cagttgcaat aataatgttg cttgctgtaa tgtttctatt cgcaacggta
     2641 acagaatttt cggaagtaat ccgagcctcc ccgttgatga tttcaacccc attctttttc
     2701 agcattaatt caaccccgga ggtgagctgt ttggcgactt gcagggctct tttgcgcgct
     2761 tgttcccagc tgaaagtgat gttttgctcg tcaataccat caacgccaaa aaatcgggca
     2821 tcgtctttaa ttcgcctgta cagcctgccg ctttcgatga ttgccttaga gggaatgcag
     2881 ccccagttaa ggcacattcc tccaatcagc ttcttttcca caatggcggt ttttaaaccc
     2941 acctgtccgg cacgtatggc agccacatat cctgccgggc cagctccaat aactactaca
     3001 tcgtaatcca tatcaatatt ttaaagttta ctccatgatt agcgaaacgg gatcggcaag
     3061 atacgcaata agcctgttga ggaatcgcgc cacctctcca ccatccacaa ttctgtggtc
     3121 aaccgaaagt gaaagtggga gtaccattcc cttggcgagc tcgccatgac ttataaccgg
     3181 cttctccacc agccttccta tacccagtat tcctacctgc gggtagttaa tgacgggaac
     3241 ggcaaattgc cctccaatgg aaccatagtt ggtaactgta aaagttccat ccttcatatc
     3301 gtcgagggtt agcttacggt tacgggcttt ctcggcaatt tcgtttacac gctcggcaat
     3361 ttcaaaaacg ctgagcttat caacacctct gattacagga acaaccagtc cttcctcggt
     3421 atcaaccgct ataccaatgt tgaagtactt cttgtagatt atccggttgt tctccaaatc
     3481 gagctgggag tttaacgttt tgtggtggct aagagccacg gccagggctt tcaggataaa
     3541 tggcaggtag ctaagcttaa cacccttttc ggcaaatgca tccttatact ttgcccgaat
     3601 gcgaaccaac tcgctgactt cgacctcgtc gaacacgctc atgtgggcgg cattctgctt
     3661 cgactgaatc atgttccggg caatggtttt ccttatctgg gtgagcggtt catattcaac
     3721 aagttgctcc ccactggcta ccattgtggc tgaaggctca acagaaaggc ttttttgagt
     3781 acctcccttt ttaagaaagt tttcgatatc ctgtgtggta accctacctg aaggaccagt
     3841 cccggtaacc ctattgatat ctatacccaa ctcctttgcc attgcccgag caactggcgt
     3901 tgctagagcc ttgcgcccaa ttttactatc tgcagcaggt tgttgttcaa aaccttcgtc
     3961 tcctgcaggc aaaaaggcgc tatttccggc aacctcaagg gtaccaacaa cccctgcacc
     4021 ctcctcaaca ggttcttctt ttcggacctc agcaactgct gcttctccct caacgccctc
     4081 aatatcaatc tcaacaagcg gggagcccac acgtatagtt tctccaatct taccgtaggt
     4141 ggcaacaatg gttcccgatt tgggagaagg aatatcggtt accaccttat cggtttccat
     4201 gttaacaagc gggtcgccgg cttcaacatg ttgtcccttc tgcacatacc actcggcaat
     4261 tgttccttcg tcaaggcctt cgccaatatc cggaaaatta aatatatacc tcatgtttag
     4321 tagtttaaac tttcaagcat ttaaaatttc atcgttttta ggatattgta gtatatcttt
     4381 tcgggtgaaa tgatatagtg atgttcgcca cgaggcaggg gcactatggt gtcaaaacct
     4441 gcaacacggt taaccggagc ttcaaggctt aggaaggcct cttcgttaat aatggcggta
     4501 agctctgcgc ctgttccaaa ggtaagcggc tcctcatggg ctattaccac cctaccggtc
     4561 ttccggacgg attccacgat ggtttgcctg tcaatcggat aaatggaccg gaggtcgatg
     4621 agctccactg caattccatc ctttttagcc ataacaattg ctttttgagc ctcgcgtacc
     4681 atggctcccc aggcaatcaa agtgatatct gttccctgct gaagaacttt ggccttcccc
     4741 aatggaatgc tgtatttttc ctcgggaacc tcctgcttaa tggcccggta aattcgcttg
     4801 ggttccatga agataatggg atcttcactt tcgatagccg aaattagaag cccctttgca
     4861 tcgtaaggag ttgagggaat aaccactttt actcccggaa tatggccgta aatggtctcc
     4921 atgctttcgg agtgatgctc cagcgcattt attccaccac cgtaagggaa gcgaatcacc
     4981 atgggcattt tgtagcgacc acgtgatcgg ttgtgaaacc tggccacgtg gctaataatc
     5041 tggttatagg aagggtaaac aaacccacaa aactggagtt ccacaatagg tttaagcccg
     5101 ttagcagcca tccccacagc agtgcctaca attgccgatt cggcaagagg tgaatcaaaa
     5161 accctgttag tcccatgttt cttctgtaac ccctcggttg ccctaaatac accaccctca
     5221 acacctacat cttccccgta ggtaactatc atagggtctt cttgtaactt tatatccaag
     5281 gcattattta ttgcctgaac cattgtcatt actttcatct gcttaatccg tataagtgat
     5341 tgttaatcta tttattccaa atcttattgt aatcaaaagg tggttgcccg aatgatatac
     5401 ccctatcaca acaggctata actctaattc gttgccattg ggatatttca ttttaaactc
     5461 tccttccatt taaggaattt ttcatgctca atcttctgct gcttcagctc atcaggcata
     5521 tcaacatagg tgtatttaaa cacatcctca agcgggtatg gatggtagtt ttcggcctcg
     5581 ccaaactgcc gttcaacctc ttgcttaaat tgggatatca gctcaccctc ggaagaagca
     5641 tcgtaaagct tttgcccaat aagatacttt cgaagccggt caataggatc ggtagcagcc
     5701 atggcctcct cctcctcctt ggaacggtat ttagttgggt catcggaggt ggtgtgggag
     5761 ccgcggcgat aggtaaacgc ctcaataagt acgggtccgt tacctgccct ggcatgcgcc
     5821 gaggcttcct gcagcgcact ggtcatggca agtaggtcat tcccatcaac ctttatacca
     5881 gggataccaa aagcatatga tttaaccgca atgttgaccg accgggtttg cattttaaac
     5941 ggaacggaga tggcatactg gttgttttgc acgataaaaa ctaccggtgc tttccatacc
     6001 gaggcaaaat tgacagcttc ggagaaatcg ccctcggaag tgcctccatc cccaacaatg
     6061 gcataaacta cgctatcctc cttcttgtag ttgatggcat agccaatacc tgcggcatgt
     6121 tgaagctgtg aggcaatggg cacagaaatg ggaagaatat tttgtgcatt cggaaatacc
     6181 gttccatcct cataaccaat gaagtagaga aaaacatctt ttagggttgc ccccttggcg
     6241 agccaggcgc caagctcgcg gaatgccgga accagccaat cctgctccct catcacaacg
     6301 gccaggccac ccgatatggc ctcctgcccg aaattaggtg gataggtgta catgcgtccc
     6361 tggcgctggt acgaaacggc catcaaatcg gcctgacgag caaaaagcat gtccttatat
     6421 gcttttacca accgctcggg ttcaatgttg ggtttgtact tggggttaat aaccctaccg
     6481 tcgttgtcca ttacctggaa catcctatct tctaatggtg aaaaatctga aagcatacta
     6541 ttgatgaatt tatttgtttt tacgtaagtg ttggtgttct aatcgttttc attaacaacc
     6601 atttaattga aaatgttcat taaaacatgt tgaggtgtgc tgatttggca tgaatagcat
     6661 taagttttcc caaggattac aaaacaccag cttgttccac agtgattaaa attttataat
     6721 cgtccaatct agctggtgga agcgggcaaa atattttgca ttacctttac ctgtctaata
     6781 aaaactgctt ccatgaagta ctccgtggtt attcctgttt acaataggcc gctagaagtc
     6841 aaggagctta tggaaagcct taccgaacag gctttcaaag atttcgaggt gctggtggta
     6901 gaggatggtt cgtcaattcc ttgtaagcag gaggttcttg aatacaaagg ccgtttgaac
     6961 cttacatatc ttgaaaagga aaatacggga ccaggcttaa cacgaaacta cggcgcctgt
     7021 tcagcaaatg gtgaatatct gatatttttc gactccgact gcctcattcc taatggatac
     7081 tttcaggagg tagagaagga gctggcctcg gctcctgctg actttttcgg aggccccgac
     7141 cgtgcacatc cttcattcac ccccatacag aaagccatta gctactccat gacctctttt
     7201 cttaccacag gcggaatacg tggaggtaag cataaactcg acaagttttt gccccgtagc
     7261 tttaacatgg gtgttaccgc agaagccttt agaaaagttg aaggattttc cgccatgcgc
     7321 tatggcgagg accttgactt aagcctgcgg ttggtagcag caggatacag ctcccgtctt
     7381 ttccccgggg cttttgttta ccacaagcgc cgtaccgatt ttcgaaaatt ctaccggcag
     7441 gtttttagct cgggctttgc caggataaac cttcatgttc gacatcacgg ctcaatgaag
     7501 ctggtgcatt tgctcccttc cgcttttgtt gtaggcattt gcctgttgct ggtcgcttct
     7561 atattttttc cttggctgct gatgctgcct ctactctact gctttgccct ttttactgac
     7621 tctctatata aaggcaacag cccttgggtt gctttgctgt gcataccggc gtcttgggtg
     7681 cagctgttcg ggtacggcct agggtttata gctgcatgct ggaagctaac catgaggggt
     7741 aaaaattatt ttttgcctga ctaagtagca aatttgagtg tcgtttaaat aaggtcattg
     7801 gtggttgaca ttataccacc tctcaggaag attagctttt cactcattct agtaacataa
     7861 tggacatcca ctaatactaa cacaaagtac ctttaaaatc aggagtaaca accttacgca
     7921 tagctatttt agtaaagtct tctttcactt atcagcccca gtagctgaaa aaacttccca
     7981 aacccatcag gtaaggttgt aacctgaagt agctatccca aaaagcaaaa taataggatg
     8041 caaaaaatta agcgcctacc tcttgcaact ttcaaaaaaa agacagtata tttgtttaga
     8101 gataccaaca gctggcaaat tgctcgaaaa ccaatccatt tccgattttt taaaccggtg
     8161 ttatgctagt atgcatcagg gcaatatcat tattgcatac aagggctcgg ttaatgccga
     8221 tattattgcg gatacgctta gcctggttga ggtaaaactt aacgacgaaa tggagcatag
     8281 ccttacacga aaaaaagttt acaacgtgtt agtagagtgc cttcagaact tgtttcacca
     8341 cgttgacaca gtgcccgggt tttacaatga ggataatttt ggagtattta ttctttcaaa
     8401 aattgatgag ggtttcaggg tttcaacagg caatatggta aacgggcaaa aaaaagaaat
     8461 tcttaagcaa aaaattgaca agattaattc tctggacaag gaagaactca aagaatttta
     8521 taaatttgtt ctcaacaatc aaatgttctc tgacaaggga ggagggggat tagggctgat
     8581 agatattgcc cgtaaaacag gaaacaagct ggagtacacc ttcacacctt ttgatgagtc
     8641 gtttgatttt tttaccctga gtgcaacaat taacgactaa cgaactaaaa cgaacaccat
     8701 ggaattgcta agcatcgaag gaactcctaa aacaccaacc gttaccatga accccaacac
     8761 aggggttatt gaaataaaag ggagatctat tcccgagaat tcaatagaat tctacaagac
     8821 tatcattgat tggattgatg agtactcaaa gtccccccaa cccaacacgg tggttaacat
     8881 tcagtttgag tacttcaaca cctcatcatc caagtgcatt ctcgatgtat ttaagaagtt
     8941 tgaaatgctg aagaagaaca aggcagatgt ggtaatcaac tggtactacg aggaggatga
     9001 cgaggacatg cttgaagcag gtgaggatta cgaatccatc atcaaggttc cctttaaaat
     9061 gattcagatt gttgattaaa acgacagagt atcacaatta taaagggcgt ttatcgccct
     9121 tttttacttc atgaactacc acgggctaag tacaaattta tgagctttac ggacaatcct
     9181 attaaatatc aaccaatccc tacattccat gttaccaatt attgattact tttgttgttg
     9241 atgatgaaaa taccacaagc atgccggtta ttaaaaaaaa tgtttcgcta aaaccctata
     9301 atacatttgg tgttgatgtt tcagcatcct actttgtaaa ggccaacacg ctggagaaaa
     9361 tcctttacgc aataaatttt gcatcgtaca accagcttcc aatctttatc atcggagggg
     9421 gtagcaacat gctgctcacc aaaaacctaa acggcgtaat ggttaatcct tttattcaag
     9481 gtttaaccct taccgaggaa accgactccg atgcaatcgt aaaggttggt gccggaatga
     9541 cctgggatgc attggtaaac atagctgtcg atcacggatg gggtggaatc gaaaacctat
     9601 caaatattcc cggaagcgta ggtgctgcac ccatccagaa tattggtgcc tacggggtag
     9661 aggccaagga tgctattttc aaggtcgagg caattgagat aaaaacacgt aaatcactgg
     9721 aattttcaaa tagcgaatgc cgttttggct accgcgatag catattcaaa caggagctca
     9781 agggcaaggt tattgttacc cacgtttggt ttaaactatc aaaaaaacct gaataccagc
     9841 tagcttacgg gaatcttaag gaggaggttg aaaagcttgg tggcgtttcg ttaaaaaata
     9901 tccgccaggc agttataaac attagaagcg ccaagctccc cgatccggct gttatcggaa
     9961 atgccggcag ctttttcaaa aatcccgttg ttgaggatgt gctgtttcaa aaaattcaac
    10021 aggactaccc cgatgtaccc catttcagcg tgtcggaatc ccatgtaaaa attccagccg
    10081 gatggcttat tgagcaatgc ggttggaagg ggaaacgggt aggcagctgc ggcgtgcacg
    10141 acaagcaagc gctggtgctg gttaactatg ggggtgcaac aggtgaggaa atattaaccc
    10201 tagcacgctc aattcagcaa tctgtttacg acaggttcca catcaacctc gacatggagg
    10261 ttaatatttg ctagccacgt tagaatagac aaataagctg atgttgaata gcttacaaag
    10321 gtaaagtttt cttcttcaac caagttttgt gttcctcatc aagatagggc gacaattttt
    10381 cgtaaaccgt tgcatggtag tgattcagcc agtcccgttc atcgtcgcta agcatactta
    10441 ccgcaattgg cttggtatca atggggcaaa gggttaacgt ttcgaaagtt aaaaaacggc
    10501 cgaagtcgtt ggaaatatcg tcggtgcaaa gtataaggtt ttcgattcga atgccatagc
    10561 agccagaaat atatattcca ggttcatcgg acatcaccat gccgggctca atgggaagat
    10621 ggttttcggg acgaatctgc tgggggccct cgtgaacgtt cagaaagtac ccaactccat
    10681 gacctgttcc atgcccatag gtaagatggt ttgaccaaag ggccattttg gccagcgtat
    10741 cgagctgcga accacgtgtt ccgaagggga acctggcctt ggcaaggcta atcatccctt
    10801 ttaaaaccag cgtgtaatgc ttcatctcgt cgggattagg cttacccagg tgaacagttc
    10861 gggtaatatc agtagtcccg ttcaggtatt gccctcccga gtcaataagc agaaatccgt
    10921 cctgttctat ggtatagtca gactcgggag ttgcgctata gtgaactatt gccccatgat
    10981 ccctataacc agcaatggtt tcaaaacttt cgcttacaaa atgctgttgt ttgctcctga
    11041 aatcaagcaa ctttttggct accgtaagct ctgttaacct ctccctcccc aaaacctcct
    11101 ccaaccagta gaaaaaatta accagggcaa ccccatcctg caccattgcc tggtgaatgc
    11161 cttcaacctc tggtttctgc ttttttgcct taatttcagt tacaattcca attttctcga
    11221 caatctttac cgtttggggt atctggttgt acaacttgta gttagtttta tcggaatcaa
    11281 ccaccaacac cgtgtgcctg ctgatgttag agataaaatt tccaacctgc tcatagccca
    11341 ttatagaaac gcctgcactg gtaaggaaat tagaaatttc atcgtcgagt tttctcccgt
    11401 caacaaaaag aatagcccgt tcaaggtcaa cataggcata gcaaagcact accggattga
    11461 actggatgtc gcttccacgg aggttgagag tccagcaaac ctcatcgggg gaacacatta
    11521 gacaggccgt agcattaatg ttaagcaagt tacgacggat actttctagc ttatcaccag
    11581 cggagattcc ggcaaattca gctgcatgaa tataggcttt agcagtagga agtggtggcc
    11641 tgtctaacca tactgtgctt gcaatatcgt catcaaaact acatttaaag ccaagctgtt
    11701 caaggctttt cgaaagatat cgtgcagcag aaaccgaaat aatgccacca ttacacccaa
    11761 ctgttgaccc agcttctaga ttcaacttta tccagcctgg aatatccggt gtatcgggaa
    11821 gcccctgctt acaaagttca atgcctgtac ctgcaagctg ctgctcagct tgaaggaagt
    11881 agcgcgagtc ggtccaaagt gctgcttttt tttcggttag caccagagtt ccagccgatc
    11941 cggaaaatcc ggaagcccat tccctaacct tccagtaatc gggaatgtac tcgctgatat
    12001 gggggtcgct atgatgaata acataggcat caaaacccct gcttttcatc tgccggcgca
    12061 gcagctgcaa tctttcattt attttagtat ccattcaatc tatttattaa caaatcaaca
    12121 tcatgtgtta tgtaaaatca ggtacccaga aaaatttcca aaacttcgta gaatacttta
    12181 ggttcaattg gcttagtaat atacccatcg catccggcgg cctcggcctt ttttcgatcg
    12241 tcagctgaaa tgtatgcggt ttcagcaatg actgggattt taggattgat ctgctttatc
    12301 tccatggttg cagcatagcc atccattacc ggaagttgaa tatccattac aaccaggtgt
    12361 atggtttgat tgatagtgct aaccaggtta acagcttccc tgccgttttt tgcccacagg
    12421 gttgttgccc cacacgaaac cagcaactcc ctaaacagca ggtagtttac ctcctcgtcc
    12481 tcaacaatca ggataacctt gtttttaaaa tcgggataaa cgggagcatt gtcctcgatg
    12541 ttgttattac tacccgaagt actgctgtcc gttaggggta atagaacttc aacctccgca
    12601 ttgtcgttgg agccgatatc gaaccgcatc tccccaccaa gcaatgtaac aatctgccta
    12661 gccatcctaa tgcccagtag agattgcatt ggcgactcta tgttttcgga ctgtctgaac
    12721 tgatcgagta agctttcgtt aacctttgat tcggcaccag ctccgcttat tcttaaaaag
    12781 atcatcacct tgctgttttc ttttctgcag ctcagctcaa ttgtgccccg ttcaacagtt
    12841 ttaaagatac cgtccaaaag gttggctaat gcctgttcca accttccctc atcggtttta
    12901 agaaagatgt tttcggcatt tcctggttgt ataaatttta gctttatccc ctcattctgg
    12961 taaagttcga actgctgtga aaaagagcgg tggagctgtt gaagcaggtg gttaagatta
    13021 aaagttgatt ttaccagagt caggttgccc ttttcaataa ggttataatc aatggtgtcg
    13081 ttaaccagcc ggagaagttg cttccccttg ctgttgatta tcccgatgta ttttttaagc
    13141 aaatcatcgg gttgattcag ctccagcagc agctggctga aacccaaaac tgcattcagg
    13201 ggggttctta gctgatggct tagctggctg aatagctcgt ctttctgctc aatttttgat
    13261 ttaagtgtaa aaatttgttg ctgtaaaccc tcagctgtat caatccgttg aatggtttta
    13321 acagcaacag aagccagctc ttcgagtaag gttaaatcgg gttcatcggc aggttctggt
    13381 ttaagtagga ggactccccc gtacatgtgg ttgccagaaa ccaaaccctc tgcatatatc
    13441 gcctcaatat tgatgtatcg ttcaataatt tttgatgccg acatggaaat atatccttcc
    13501 ataataccgg taaacgattc gttaaccctg taggctgcag ccgacttaat ggcatgttta
    13561 tccttatcgt gcagtaggaa ccggcggcca acagggttcc atcccaagtc gaaaattaac
    13621 gattcccagg tcttgtgctg aatgccaaaa atgccttcaa ccgtaataaa gccatcggca
    13681 gcattatacg acaataccac tgttaccatg ctggggaata tccgctcaag ctgttgccca
    13741 aagcacgaaa aaacaccctc gaaattagca gcaccctgca actcggcatt agccaactta
    13801 atagcctggt acctttgttg aagtaggtta acttcaaaat ttacctgaac ctgcctggtt
    13861 acatcctgcc caattagggt attgccttcc ataccaacca cttccggaag tttaagcaga
    13921 atccacgaaa tatctttaac ctgtccttcg cggtttctaa ctctacacac aaaaggttta
    13981 gaacctcctg aatttgcata ggtaaccagc tgtttaaaag aatcggcgtt tgtgaaaaga
    14041 gacggtaata gctgtgggtt ctggagttcg gccaggctgt agccaaaaga tttttcggta
    14101 attcggttgc atcctttaat agcgccctcc ctgctaagct caatcatcag caaacgatgt
    14161 gacgaagcaa ttgatgagta aaaatgcaga aaacttgatt tatcagcaat tccacaaagt
    14221 ttggatgtat caatcatcac cccctgaata tacctgcttc cttcaacctt cctggcaagc
    14281 agggtaatga ttcgtttggc gttctgagga cttgaaaggg ttaagttccg tgtaaaaaat
    14341 tcttcatttc cgttggcaaa aacctgccag ctttgcgaga gctccttgaa gctggagggt
    14401 gaaagaattg ccctaaattc tgaaaaattc caggttttac cttttggtgt atttccccaa
    14461 aacatagaac taggttgaaa ttgccattgc agctggcctt cctccgggtt ataggtaaaa
    14521 gcatcaaagc ccataaatgt tgatgcaggt atggctggcc cggatatggg ttgcctggga
    14581 tcagcaggta aaaattttcc aaacaggctt tgcacactcc cgtcggtagc tatttctcct
    14641 actaaagcat gaattctcaa cctatgccat acgtcgttca ccattaccct aacatcaaat
    14701 tccctgtagc gtaacttttg cttcagcaat cggaaaagga tagtagaaag ttgccttcta
    14761 tcgtcggggt gaactacgct aaagaagtcg tcaagatgga tgcccagctc atcagcctta
    14821 actccaagtg cctcttggaa tttatgccct agcagcaagt ggttggtttc tgaattccac
    14881 agccagaatc cagcccttag gtaacgatgg atatgctgta ggtagatatg tgtttcagac
    14941 tctttgcttg ataagctatt tccattcatt tttacagagg aaaatccttt aacagtatgc
    15001 tacaatgatg aaccaattta gctttttact ttacaattta taaaaatagc aaagttaggc
    15061 taaactctca acattttttt aggaaaacaa aagctattgg ttagaattta agctgcaacc
    15121 tgatatttaa gcgccttgag gttaggtagt tgggaacagc aaaggtatct gattcaccct
    15181 gctggttgcc aacggtttga atccagaggt aggaaaccgt gttgttgaaa ttaaacaggt
    15241 tgaaaacctc ggcgcccaac catatcccct taaatccttt caacttccgg gatataccat
    15301 tagaagaaat ttcgtcacgg agtatcttgg taaaaccgat gtcaaccctt ttgtaatcct
    15361 tcattctgaa ggtaacatca tacctgtcga taccgttcac accaacaggc aagcctgtcc
    15421 cgtagttagc gctaaggtgt accctgaaag tcggattacc gggaaggtaa tcctggaaga
    15481 acaagttaaa ccccagggtt tgatcggttg gtcggggata gtaccctggg taaaccgttt
    15541 gtccgttggc gtcaacatag ctatcgtgaa gaatatcctc catggtttta agtagcgaaa
    15601 tgctaaccca cgactcggca tcctcaagca gctcaccatt aagcttaata tcaacgcccc
    15661 gggcaaaacc cttagccagg ttttgtccag cataacgaat tcgcacgttg tcaactttat
    15721 aggggataag gttgtcgagc catttgttgt aaagttctac cgacagctta aaaggtcggt
    15781 tccaggcagt aaagtagtat tccaaaccag caagcagctg caccgaacgt tgagctttga
    15841 tatttggatt taagcttccg ttgggcattc gcagctcccg gtaaaaagga ggttggtagt
    15901 aaacgccacc cgctaaatgg aagttaaaat ttttaagtct ttgattttta agcgataacg
    15961 agactcgggg acttaccagc acatcaccgg tataatccca gtagagaaga cgtagtccgg
    16021 tatttgtggc aagggtaaat gcccctctgc ttaagctgta atcgagctgt gaaaaagccg
    16081 ataacttatt caccgaggtt gagttacctg ctaccatcac atttgaaagg ccaatggctg
    16141 aaccattata gggaatggca tagcctgagg agtcaagcag attccactcg ttcagttcat
    16201 cgtcgaagaa ctcatgctga agctgggcgc cccattttag cttggcattt gaaaacctca
    16261 cacttccaac aagcccaaaa ttattgatgc gggcaaagag gaagttgcgg gcatgattca
    16321 gaaatccacc gattcctacg ttaatcagac tgtcgccata ggaacccgat cccattgagt
    16381 tgtcaagctc attcaggaag tattctccaa gcaggtcgaa tgtttcggac tcgcgagtaa
    16441 ggtagtttgc agagtaaatc tttatgctgg tgttttggct tggggcatac tccacataag
    16501 cggctcccat tcccgaaatg tacctgtcca gctccttacc ctcgtagtat attttcagct
    16561 gaagcgcatt gctgaacacg ccaaacctgg tttctctaac atctggagca aactggtagc
    16621 tgttggtggc ataattgccc agaaagctaa acgcaagctt atcattcaac ctatacgaaa
    16681 acaacccctg ccaatcgagg aatgaagggg tgtagtcgcc ctttacgtca agcgtgttca
    16741 gcaggtagct tgaagttttg tagcgcactc cggttatgta cgaaaacttc cggttaccca
    16801 aaacaccctc ggtgtatgca gaggcaccaa gcagacttag agacacacca gactggtagg
    16861 aggtcggcat tcggtagctc acattcagaa ccgacgacat cctgtcgcca aactcggcgt
    16921 taaatgctcc ggcagaaaac tctacagccg ataccaaatc ggaatttatg aagcttaacc
    16981 cctcctgctg ccccgatctt actaaaaatg gcctgtaaat ttcaatatcg ttaacgtaaa
    17041 ccaagttctc atcaaaattt ccccccctaa ccgagtactg cgagctaagc tcgttggttg
    17101 atgccacgcc cggcatcagc ttgataatgg attcaaaatt tccagaaaca ttgggcataa
    17161 agctaatatc cctggcgtta accctttcga tgttacccgt ccagcgacgg tcggacgata
    17221 cacgaatctc atcaatcagc aactcgttaa catctagctt caccctaagc tcaacaattg
    17281 ggctatttac gtctgcatta ataagaaccg aagtgctttt gtagcctaca cacgaaatgg
    17341 tgagaacagc actttgcgca gccggaaggg taatgcgaaa ccgtccattg atatcggtcg
    17401 aaacccctat tggcccaccc tctacaacaa cgctggcaaa aggaacaggg ctgccaaccg
    17461 agtcggttac aaaccctaca accaggagct gacccgtctg gccttttaca accataacgg
    17521 ttaccattaa caacaggagc aaccctgctt taaagaaata gaaagcagcg gtaaccttcg
    17581 tcttatcctg tttttcgggc attacaagtt gttgagttta tctgtacata ttccctcaaa
    17641 agtagcatgc tgaacaatac caagcctaag aataaccata tttgcttaaa aaaattgtgc
    17701 tacaatagct gtcaacatgg tttacaaatc aaatttcctt acctttagtt gattttttaa
    17761 tttactcccg ctttcgattt acctaattgt gaaacataaa atgcctgagc atgattcaac
    17821 aggttgaatt tgccctcccc aaatttccaa ttggttttca cctgataacc gagttggttg
    17881 ttaaaaaatt gccggcgtta cccgaaaccg gaatggccaa catattcatt aagcacacct
    17941 cggcagcgct aacaattaat gagaatgccg attacaccgt acgaaaagat tttcagaaat
    18001 ttatatccac ccttgtccct ttttccaaca cctacgagca taccctggaa ggtgaagacg
    18061 atatgactgc ccatattaaa tcgtctctcc taggccaatc ggtttggatt cccatctcta
    18121 acggaaagct gaaccttggc acctggcagg gaatttacct atgcgagttt cgaaatcatg
    18181 ggggaaaccg taagctggtt attactatca tttcctgacg taacattacc taccttaaat
    18241 tttaactttt catcaaaaaa gggcttttag cttaatcatt ctaaaataat tcctactttt
    18301 gctaacaatc agaacccaat tttgaactga atggtttaaa actgaaatga tttaaccatg
    18361 tattcggcct attacttata taacgttgcc cagaacgtag gttacctgaa gcgttttggt
    18421 ggcctttaga acttgtttag cttataccat taccttaata gctaaccacg ccaaagcaaa
    18481 agccaaataa caattttgaa gaataaacca aacgaaatgg aaaagttact acttctaggc
    18541 gatgaagcca ttgcccaggg agcaatagac ggagggctat cgggcattta cgcctaccct
    18601 ggcaccccat caacagaaat catggagtat gttcagggtt caaaaatggc tgccgaacgg
    18661 aacattcaca ggcaatggtc ggccaacgaa aagacagcca tggaagctgc tatcggcatg
    18721 tcctatgctg gcaagcgtgc aatggtgtgt tttaagcacg tagggctcaa cgtggctgcc
    18781 gacgccttta ttaattctgc cataacaggc gttaatggag ggctggtggt agtatcggcc
    18841 gacgatccct ccatgcactc atcgcaaaac gagcaggact caaggttcta tggcaaattt
    18901 gccatgattc ccattcttga gccatcaaac cagcaggagg cctacgacat ggcctaccaa
    18961 agttttgaac tctccgaaag gcttaacgtc cccgtgctgt tcaggattac aacccggatg
    19021 gcacactccc gcgccgatat tagcaggcat gaagttaagg atcaaaacaa gcttagaatg
    19081 ccgctcgacc caagacagta cgttttactt cccgcaatag cacgtaaaaa ctataaaaag
    19141 cttctttcca cccaacctgc tattgagctg gaggctgaac aatctaaatt taacgccttc
    19201 attccaggag aaaaccacga catgggtatt gtagctaccg gaattgccta caactacctg
    19261 gttgaaaact accccaacca ccagtgtccc tttccggttg tcaggattgg acactaccct
    19321 attccccgca aaaaaattac cgagctctac gaaagctgca aatcgctgct ggttattgaa
    19381 gagggatacc ccatggtgga agagatgctt aggggatttt tcaatgttgg caagccaatt
    19441 catggacggc tcgacgggac cctgccccgc gacggcgagt taaatccaaa catagttgcc
    19501 aaggcattag gttttccggc tgctggtgaa gagcctttgg cagaagttgt aaaaaaccgc
    19561 cctccatcgc tctgcatggg atgtggacac cgtgatgttt acacggattt gaacgaggca
    19621 attaaggact acagcgccgg ccgggttttt tccgacattg ggtgctacac cctgggagct
    19681 ttgccaccat tcaacgccat aaattcatgc gttgacatgg gagcctccat tacaatggcc
    19741 aaaggggcag ctgatgccgg acttgttcca gctgttgctg tcattggcga ctccaccttc
    19801 acccacagcg gtatgacagg gctactcgat gctgtaaacg aaaactcccc tataaccatc
    19861 atcatctccg ataattcaac tgccggtatg accggaggcc aggattcttc ggcaacaggt
    19921 aaaattgttg acatttgcag gggtatgggc gttgacccca gccacatcag ggtggttgta
    19981 ccgttaaaga aaaaccacga ggaaaacgtc aagataattc gcgaggagct agcctataat
    20041 ggcgtttccg taataatccc ccagcgtgag tgcattcaga ctttcgcccg taggaaaaaa
    20101 acagaaaaga aagaatcaaa tgattagcaa tgagctggag aaagtttacc atgcatcttt
    20161 ttcggatgaa cgtagctcag caattaatca atatttctaa caacgtatta tatatcaact
    20221 agttaaacaa tttataatga ataacgatat aattttagca ggtgttggcg gacagggaat
    20281 tctttcaatt gctgcggcaa ttggcctggc cgctgttaac agcaacctgc atatcaagca
    20341 atcggaggtt cacggtatga gtcaacgcgg gggtgatgtg caatcgcacc ttcgcatttc
    20401 cgatgcaccc attgcctccg acttaattcc cgccggaaaa gcccacctga ttatctcggt
    20461 tgaacccatg gaaggcttac gctacatgcc atttctgtcg aaaaacggat ggctggttac
    20521 caacataacc ccctttgaca acatacccga ctacccaaag gtggacgaga tactaaagag
    20581 ggtgaaagcc gttaaaaacc atattgccct cgatgccgac tccattgcca aggagtgcgg
    20641 gtcaacaaag gcttcaaaca tcgtcatgct aggggcagcc tcacctttta ttgatattcc
    20701 tgccgctggc atagaggagg gaatacggca gctatttaac cgtaagggag aggatatcgt
    20761 caaccttaac cttaaagcct tcagggctgg cagagaatac agcgaaaaga accgctaatc
    20821 ctctattacc ctgcaagagt atacgttgtt taacccactg gaaatgaggg ttacaggttt
    20881 tctcagaaaa taggtcggct acattacaca ttatcgaaaa actccagcga cggctggagt
    20941 ttttttttta cgcattggta aaggtaagca ggtcattatt tttgcgtcct acagcaaatt
    21001 ttcatcccgc ttgcagcaaa actcacaaca tgtcgtcgtt atgttgttgt attgaaaacc
    21061 ttaaaacata agtatgtata agttgctgcc cccattctgc attgcaatac tgctatttgc
    21121 tgttcaacct gcccaggcat tccttgatga ggataagaaa atttgccagg ccgtccgggt
    21181 aaatccaaaa ccccctgttg ttgacggaag ccttgacgat gaagtatggg aaaagggtga
    21241 atggaacgga aattttatcc aacatgagcc ctacaacggg aatgagccca gccagaaatc
    21301 ctacttcaaa attctatacg atagcgacta cctgtatgct gcctttaaga tgagcgatag
    21361 cgagcccgat aaggttgtat cgaggataac ccggcgcgat gatgccgacg gggatagggt
    21421 tacactctgc atcgactcct actttgacca acgtactgca ttcctcttta caaccaacgc
    21481 tgcaggagtg aaaagcgatg aggtgatgac cgaaaatggt gataaggaag atatgaactg
    21541 ggatcccatc tggtgggcca aagcggttaa aactcccgat ggctggacag ccgaaatgaa
    21601 aataccgtta aaccagcttc gtttcgctaa ctcatccgac atgaaatggg gcgttcaggt
    21661 gatgcggtac atttaccgca accaggaaac tgacctctgg cagcatgttc caaaggatgc
    21721 acctggattt gttcaccttt tcggcgagct gcacgggcta aacggacttg cccccaagcg
    21781 gcaggtggag gtagcaccct acatggttac caaaaccgaa cgacttgagg ctgaatctgg
    21841 aaatcccttt atgaccggta aaaataatgg tttcaatgct ggcgttgacg caaaaatagg
    21901 aataaccaat aacttcacac tcgacctaac cgttaacccc gattttgggc aggttgaagc
    21961 cgaccctgcc gtggtaaatc tttcggcttt cgaaacctac tttgaggaga aaaggccatt
    22021 ttttattgag ggaagcggca tgatgaactt ttcgttgatg tttggcgatg gcgacctggc
    22081 aacccagaac ctgttttact cccggagaat tggtcgcgct ccccagcttc aacccgattt
    22141 ggacgataac gagtatgcta aaattccttc aaacactgca atcctcggcg cagccaaggt
    22201 taccggacga accggaaaag gcatgtcggt tggtattctt gaaactatta ccaacagcac
    22261 ctacgccacc atcgacagta tgggtgttcg cacacatgaa atagcggaac ccctcaccaa
    22321 ctacacagtt gccagcgtgc ggcaggagtt tagcggagga aacaccatca tttcgggcat
    22381 agctacttca accaaccgcc agctaaacga tctctccagc gactactttc atcaaaatgc
    22441 ctttaccggc ggcatggatt tacagcgatt ttggtcgaat aagaaatacc tgttttgtgg
    22501 aaaactctat tttagccatg ttaacggaag caaggaggct atagccagaa ctcaaacttc
    22561 tccaactcgc tactttcaaa ggccagacaa caactatgcc aggctcgact ctactcgcac
    22621 gtcgcttacc ggtactgggg gattgctaca gtttggaagg gttggcgaag gacacctgca
    22681 gtacatggga gcggttacct ggcactcccc ccaactcgaa attaacgata taggatacat
    22741 ccagaacgtt gatgatatct ttcaggtgat gtgggttgga cttaggttct ggaaacctac
    22801 cctgtggttt cgctatgtaa actttaacct taaccagtgg gctacatgga actttggagg
    22861 gcagtcaacc gataaaggtg gaaatattaa cttcaaccta cagttcacca actactggac
    22921 atttaatgcc gggattaact ataacagcga gtctctgagc gcccatgccc ttcgtggtgg
    22981 tccaatgctg aagataccag gtaactggaa tgcatggtat aatataggga ctgatgggag
    23041 aaaaaagctg gttttctaca tgggtggatc aacgctggaa agcaatgaga actacgctta
    23101 ctaccaatcg gtttacgcaa ccctttcata tcgccccctt aaaaatctgc aactttctct
    23161 aaacccgtca tactccgtaa acagtgataa gctcgaattt gttgaaaatg ttgattacgg
    23221 caacgaaacc cgctatatcc agggaaaact tagccagaaa acatttgccc tttcgttgag
    23281 agtggaattt agcattaacc ccgacctgtc ggtgcagtac tatggccgac cctacctttc
    23341 aagtggcaag tacaccaaat ttaagcgtat taccaatcca atggccaaca gtttcgatca
    23401 acgattcgta acctttgagc ccagccagct tgtttacaac caggatgagg aggaatacta
    23461 tgttgacgag aacaacgatg gcggttccga ctactcattc tccaaccgca acttcaactt
    23521 tatggaccta cagtcgaacc tgattgtgcg ctgggagtac agaccggggt caaccctttt
    23581 cctggtttgg acgctgggta agcaagccta cgaatcggag tacggcagct cgttgaacaa
    23641 caacatcgaa accctttacg acgctcaccc ccataatata ttcctcctga aattttcgta
    23701 cagattcaac tagccctaaa cactcggcat tcgaatgtag gcaggaagca cctgcaacgc
    23761 tccacctgta ggtgttaatc ccaacccgca ttattcccat cagcaacttg aggaatcatc
    23821 tgaacacatg tcagcaattg cctaccgcaa tacttcatag aagttcacag atccggcctt
    23881 caaaaaacct gcagcatcac acccgtgaaa aaattcaaaa ccgacattta aaaatttata
    23941 aaaatttaat ttcaaaatac taacaaatct taaccaaaac atgtatgttt agggtgaagt
    24001 ttgcactacg attatttcac agctagccat accatgaaaa tatataccaa aacgggcgac
    24061 aaggggatga cctccctgat aggcggaatg cgtgttccca agtaccaccc caggatagat
    24121 acctacggaa cagttgatga gctaattgcc tttatcgggt tgctgcgcga tcaaccagtt
    24181 gatggccaca ccagggatat gctgatattt gtccaggata aattgatgag ctgtgccgca
    24241 gtgcttgcag ccgaagaacc caactcaaaa actgacttac caacagtttc cgatgccgac
    24301 gttaggaagt tggaagcaga cattgatgcc atggatgccc ttcttgaacc cctgacatcg
    24361 ttcgttcttc ccggtggaca tcctacagtt tcgctttgcc atgtttgcag aacaatttgc
    24421 cgtagggctg aacgactggc cgttaaactt gcaaccgaaa cgcccctacc cgacaacctg
    24481 actatatttc tgaatcggct aagcgattac cttttcacgc tttcccggaa gctggcaaaa
    24541 gatttaaagg tagatcagat aaaatggcat cctgataagt aattccaatt ttttatatat
    24601 ttgcgggtat atggttattt ataatctatt aattatcagt ttattaacta aaatataaat
    24661 tctacctatg tactggacac ttgaattagc atcaaagttg gaagatgcgc cctggccggc
    24721 aacaaaggag gaactcattg actatgcaat tcgctcaggc gcaccacttg aggtgattga
    24781 aaacctgcaa gaacttgagg aggaaactga aatatatgaa agcatagagg atatttggcc
    24841 cgattatccc accaaggagg atttcttttt taacgaggat gaatactaat cattctaaat
    24901 ggtaacaaag caaccagcaa gcaaccattc aaaagaaatt agaaaaagcc acttcggaat
    24961 ccatgtaggt gaactctctg cccccgccat gaagggttat ttgccttaca gccaaaaagt
    25021 ttttggtagg ggtagcatgt ttttgcataa cctacctgtg gaaaactgtg gaagaatagg
    25081 aaagaaggat tgttggtgtt gacccggctt tagttcgctt ccttctcggc gccaattcta
    25141 tcaaactgct ctaaaaaacg tttaagcaac ctatagtata aaataaggta gagcaggatg
    25201 tttgaaatcc aaataattgc cacatttacc cagaaagttc cagcaaattt tccaaatacc
    25261 ggcttaacag gtgagtagaa gtgtgccttc acaaactttg tatcaggata aaggaaaatg
    25321 ggatcaattt tctgaataag ccttccttta tactcggtaa ttctttccac ttcacccgtg
    25381 tttctgacaa aatctgtaag actctcgttg tagtattgcc gtttaaggtt tacaaatgct
    25441 tcctgcccac caggagcagc ctgaaagctg gatatgtagg catccttctg gctgctggct
    25501 ttgttataaa gcttcacgta gtacttgcga accagctcca ggtagctttt cagctcgtcg
    25561 agcaactcgg gtgaaactcc agccatggaa acctggttat acttactaaa agtaagcttc
    25621 gagccctgcg gcgatgtcat ttcatgcata atttcgttac gcagcagggt tagggcatct
    25681 tccacctcct cacttttttc gggcaagccc aggttccgag agcagaagtc aaccttattc
    25741 tgaagcgatt ttaaccagta gttcctcttg tagtccgaaa tgctcatggc ctcatcaaac
    25801 acgtagaagg gtttctcata cgggttatta cagaactgct caactgccag tgcctcgtag
    25861 gcccatcggg caacaatcag ctccccgtaa aatggaatgc tacttggtga ggatattttg
    25921 ggattaagct tttcgtactt tacaattacc ccgctcaaga tgatctgggg aataaccaaa
    25981 aacgggatga gaatataaat tgttacaacc gtcttgaagc tatccgagat gaccaacccc
    26041 agcatgttcg acgagaacca tgcgctgaat agaaccagcc agtactcgaa gtacatcccg
    26101 cgaacctcaa ggattgaatt cccaacaatt acaaacatga atgcctgcac tgccgacaac
    26161 accagcagta tggcaacctt cgacatcagg tagctggacc agctgaggtt caggaatgat
    26221 tcgcgtttct gaatttttct atccttaata atctcctcgg cgcttaccgt gaggcctacg
    26281 aaaactccca caatcaccga catgaacagg taaacaggaa tgttgctgtt ttccatgaag
    26341 gtgtagccaa ccctgtttga aacatcaatg ttgaagtact tgataatgta ggacagtagg
    26401 aaggccaaca gcggtgtttc tagcacattg ataatcatgt actgcgtgtt ggccagcttg
    26461 gacagcacgt cgcgcttgac aaaaattttc agctgcttaa accaaccagg ggtcttaaag
    26521 tgtattttgg gaaggaaatc gggaataccg tctagctggg gttgagctga aaaccacttt
    26581 ttgtagtggt tgttccactc tacggggctt attttacggg cacgggtagg attgccgtac
    26641 tcgtcgagca cgttggcctc cacaatgttg aatatttgtt cggggttcac attgccacag
    26701 gtggcgcatt cgctctcgtt ccagttggca tgctgaaccc ttgacttaaa gtacatgatg
    26761 gactcgatag gatcgccatt gtagatgagg tagccacccg tgtcgagtat caggagcttg
    26821 tcgagcatct tgaatatttc ggacgagggt tgatggatta ccacgaaaac aagttttccc
    26881 ttcagggtaa gttccttgag caggtcgagg atgttttccg aatcgcgtga gctgagaccg
    26941 gaggttggct cgtcgaggaa aaggatggca ggctcccgaa taagctccag ggcaatgttg
    27001 agcctttttc gctgacctcc gcttatcttt ttgttaaggg gtgtacccac ccgcatatcc
    27061 ctgatttcat taagcccaag gtttaccagt gttcgggaaa ccgcctcatc gagctgctgg
    27121 ggcgtgtagt tgtcgaaaca caagcgggcg ttgaaataca gattttggta aacggtaagc
    27181 tcctcaatga gcagatcgtc ctgtgaaacg aatccaattt gtccctccac caactcgggt
    27241 tgcctatgaa tgtcaatgcc gttgatgagc acatttccgg ctgtgggctt ttcgcttccg
    27301 ttcagcacgt tcagcaaggt agattttcct gcacccgagg ctcccataat gcctaccagg
    27361 tgtcccgatt cctcagtaaa cgtcatttgg tgaataccga tttttccact tctgaatcgg
    27421 tactcaacgt ctaaagcctc gaatactatc ctctccttga gcttatcgag ggcaaaccgc
    27481 ccaacaatat cgctgaagta aaccggtttg attttaggat tgcgaatgga agagccattg
    27541 ctcagaacaa aaattttctc gttctggatt aggtggccgt ttaggtagag ctcatgggtg
    27601 cccctgtaac gcaggaaaaa tatgttggcc gactcaataa aaacaacccg cagctcgtct
    27661 agcaagccct cagcgtaaat gtgcttcaca ccaggcagct caaaatcctt gttgccgtcg
    27721 atgagtaaaa catttggggt aataggaatt tggttgaagc tggagagaac gaaatcggaa
    27781 agcatgttga actcatcagc ggaaacgtag aatgtttcag caacggtaga aataaatgcc
    27841 atttcctgct cggtaacctt gccctcttcg ggcttaacaa actccagcag cctaaccagc
    27901 accactattt tttgctgctg ctgtagctct tcgtttatct gggcacaaat tttcagcacc
    27961 cttaccgagc ttgacgatgt tctttttttg gacttggtct tctcgctttg cttctcctgg
    28021 taaagattgt agtagttgtc gaaaaccttg atgtactcgg ccaccagctc ctggttcagc
    28081 tggttacgaa ggaattcctc cactacccga cgacggtcgc tgtaatcatt ggcttccatt
    28141 tcctcctctg caaccggtcg ggaaatgatg gcaaaaagct gcattagagc ttttagaatc
    28201 gattcgctca tggagtgcta tataaggtta gaactactgt ttaaacccaa taagcagcat
    28261 aacatatcca ggtgtaaatt gctggcttac cacatccacc tgaataatac atataaccgt
    28321 tgaagtgaag cggaaattcc agtatggtga atagtcataa tccctgttgg agaaaagaag
    28381 gtttttatcg gtgtcgtaca cagttagaac aacctgcccc tttttaacgc ttgtgcaggt
    28441 ggtaatcctg taggtatttt ctccaaagaa agtggtgcgg aactcgataa cctggtcggg
    28501 cttaagggag gcaaagtact cctgaccatc ggaaacgtat tcgggaggaa agttcagtgc
    28561 acaggtgttt ctgagatttt ctacctgggc ggtggcttta actgagaaaa gtccgattag
    28621 tatgaagggg attaaaattt ttttagccat ggcaacatat tgtcattcaa ctaatttatt
    28681 gcggatactt tcaattttgc tgcttatgat ccggatatgg tactccgaaa ccaccacctg
    28741 cgtggtgcta tttattgtga tttgcctgtt ggcagcatca ataatcggct ccttgtattt
    28801 gtaggagtaa attatgccat cgtattcgta ggcaacattg ataagctcgt ctatcagctc
    28861 ggaaaaatcg gacgatttgt agtagtaggg agttaacagc tcaataagat tatcaagggg
    28921 atgtttctgc tcgccaaacc ggttaatgat gtcccggttg ctgtttccaa gagcaatctg
    28981 ggtcagaaag tacatgctct caatccacga accggcaact attaaggcgc caatatcgct
    29041 ccgctcgttg gtcttaaggt ataaatcgga acttgagtat gctttggtta taattcgagc
    29101 taatgagtcc ttgttgttaa tattctgctc aaccgcatca aaaaaattct ggtcgaaaat
    29161 ttgctccaga ccaagctggt cgcagagctt acgtatggca tcgaggtatt taatggcttc
    29221 ctgggttcta tcgtatatgc acaggtagct gatatcggca ccgtaaatgc caaggttaag
    29281 agcctgcttg aaagatttgc cgtacttttg ataccggtcg taccggttga gcagattctc
    29341 gttgtaagga atgcttaggt tctttaccag ccctacaacc tgccaaggag agggaatggg
    29401 aaaaatagaa ctttcatact tgataagggt gggtagcgta tcaatactta catcctcttt
    29461 aatcgtttga ttatcagact tattaggatg attacattca gtaagcccaa atattaaaaa
    29521 acaacccgat acaaaaaaaa ctatgcgctt aaaattaagc atacatcact tttgaaattg
    29581 ttcagtgcaa gtaagataaa ataaatgaaa tatccaaagg gcaaaagcgg caaattagct
    29641 accccaacaa acaacgggca acaacctgat ttggtaaaaa aatattaaac ctcccggagc
    29701 agcaccatgt caaaataaaa ggattatttt tgctttagct tggtgtagga tacccgaatc
    29761 gcacacacta aatgctgata caaacactaa cttataaagt taaaagcttg aaatatgaaa
    29821 aaagttaccc tccttacagc tgcatttatg ctgtcgctaa catcggttgc ccagcagaaa
    29881 gttttgaccc ttgaacaggc agtcctaagg cagtatgccg actttagacc ccaaaccctg
    29941 gcaaatttca gctggaaggc taatgtcaac gcatacacct acactgaagg cgacagcatt
    30001 attgaggttt ccgttccaag cggtatgcct aaaaccgtta tgacgcttga attgctaaac
    30061 cgtttgctct cagccgattc aattcctgca ctgaagaggt tggcctacac atgggcatcg
    30121 gaaaacgtta tcaaggtatt ctcggctcca tacttggcct ttgttgatgt tggtttgggg
    30181 aaaatcacac gcaggtttac aattcccgaa gaggcggaaa acctggagtt aagccctaac
    30241 aataccagcc ttgcctacac agttgcaaac aacctctaca ttactgatgc taagggtact
    30301 ccggttgccg ttacaaccaa cgataacccg ggcattgtta acggacaagc tgttcatcgg
    30361 aatgagttcg gaatcaacaa aggcattttc tggtcgccca gcagcagcta cctggcctac
    30421 taccgcatgg acgaaacaat ggtaaccgac taccccttgg tggacataac gcagcgcatt
    30481 gcaagggtag agcctgtgaa gtaccccatg gccggcatga agagccacga ggttaccctc
    30541 agagtttaca accttagcac ccattcgaac atcgaaatta aaaccgggct acccgcagaa
    30601 cagtatctta cctgcgttac gtggagtccg gaaaaagtcg atttttattg caacgctgaa
    30661 cagggaacag aaccacatgc ggctcaaccg gtatagcgct caaaccggag agctgatggc
    30721 aacccttttt gaagaaaccg attctgaata tgttgaaccc caacaaccgc tggcgtttct
    30781 tgacgataat tcgggtaggt tcatctggca aagccggcgc gatggctgga accacctcta
    30841 cctttacgat gtaaacggaa agttgctcag gcagctaacc cgtggcgagt gggaggttac
    30901 cgatattaac agtgttgatc ttccaaacgg taaaatttac attaccgcaa ctcgtgaaag
    30961 cccaatagaa aggcaagttt acgctgttga cctaaaaaag gacaacatta ccatgctaac
    31021 ccagggggca gggtttcata acgcactttt cagtcccgat tgtaagtatt ttgtcgattt
    31081 ctactcctcc atttccattc caaacagggt taacctttgc acgggcaagg gaaaaatagt
    31141 taaaaacctg gtcaatgccc ccaaccctct ggccagctac aacgtatccc ttcccgaact
    31201 cctcacgctt aaggctgatg acggcacaac cctgtacggc cgtatgatta gacctgtaaa
    31261 ttttgactct acactaaggt accccgtggt ggtttacgtt tacggaggcc ctcactcgca
    31321 actggtacgg aactcgtgga tgggtggggc aagcctttgg gagtgctaca tggcttcaag
    31381 gggctacatc cttttcaccc tcgataaccg gggaacttcc taccggggaa gcaattttga
    31441 gcaaacaatc caccgccggc taggaaccat agaaattgcc gaccagctgg ctggggtaaa
    31501 cttcctgaaa tccttgccct atgttgattc cacaagaatt ggtgttcatg gatggagctt
    31561 cggaggattc atgaccattt cgctcatgct gcgtgcacct gaattcttta aggttggagt
    31621 tgctggtggc ccggttaccg attggaagtt ctacgaggtg atgtatggtg agcgttatat
    31681 ggacacccca caggaaaacc ccgacgggta tagcacgtcc gaccttaaac agtacgtaaa
    31741 aaacttaaag ggaaagctcc tcatcatcca cgacgatatg gacaaaacgg tggtgcctca
    31801 gaacagcctc acgctgcttc acgagtttgt taaaagcaac gttcaggtcg atttcttcct
    31861 ctacccccag cacgagcata acgtgatggg taaggacagg gttcacctga ttgataaaat
    31921 tatccgctac ttcgacgaca acctgtaaaa ccagctaccc tggtttttgc tacaagctgt
    31981 gcagagcaaa tgtgagatgt tcccttaagg tgaacggctg gcttcacaga acttttttgc
    32041 tgctgcacaa ttgccttcgc cgagctcccc gccgactggc gggtcggttc ttgcagtatc
    32101 ggcgttcggc ttaggctaaa gcctaagttt aatataacct tgcaggctga gcctgcattc
    32161 gataggctga cggcgggaca gcgtctaaat cccacgatag gcagtatcga aaatgcgatt
    32221 agcttgctaa tccgcatgaa gccagcattt tttacaccct ttgttagggg aagtttttat
    32281 tcatttgaaa aatatggctc tgtcttaata aatttattgt ctttgtaata aaacgttatt
    32341 ttgtttcctt taagccaatt attattcata atattttttt cgaaagagtt atcaaaaagt
    32401 tggttttctg caatgcccgt ttcgttatat ataatatgaa aaaaatagct tgagtgtcct
    32461 tcaaattctg aaattattga atcaggagta ttctctttaa aatagtcttt taattccaca
    32521 ttaaatagtt taatattaat agtatctagt gaaaatgtct tttcattgaa gttatagtta
    32581 taaatcctaa attcaagtaa ttaatgcaac tttttaaggg ggtcagggaa gtaaaaaaac
    32641 aaactttttc aagacaaagg agaaaaaaag cagattttcc ccccttgtcc tgatggagta
    32701 aatgccttac tttgccctct tccctcaaaa agttacaaag atgaagcact taaccgtaga
    32761 gcaaaggtac accatt
//
LOCUS       DAOPHX010000009        31086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_1411_length_31086_cov_6.699816, whole genome shotgun sequence.
ACCESSION   DAOPHX010000009 DAOPHX010000000
VERSION     DAOPHX010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 31086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31086
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1411_length_31086_cov_6.699816"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1576
                     /locus_tag="PK502_01485"
     CDS             <1..1576
                     /locus_tag="PK502_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99521.1"
                     /translation="LSFSSGYEVQELSFDRNTRMLYALVVTQQGKEIVSVDKNKSFNV
                     VYGPTFDDVNSIYAFDSRVIFSSTDGDVENIYLHNNLTDSTVKIYNSRYSSRYCSVTK
                     NHELIFSDYTPDGYTIGTSTIDLDNQKNDLISNRVNNSYPNFTLIGKGSVVVDTLQIS
                     DSVFSSKPYKGAGTLFNVHSWMPFFVNIDGTEVTSINPGVMLLSQNLTGTLTSSIGYG
                     YGNSHLFHTDVTYYGFWPVLSVRFNLYDEDAFLYRVTENPVFPNKNRWEAKLNVYLPL
                     QLANSSYQTYLQPFSDLTLSNDYLFSSNDSSYHSGIGTVNSGFYFHRLRNMAHRDLQP
                     RMGLVFRLNLVNAPFNKNSLGSLQAANLKIFLPGFLANQGLKFSAAWQNQNLKRYYLG
                     NEISFPRGFSENVSKKYLGASADYIFPFITPDLSLGALAYIKRISVNLFYDYARNSYL
                     WVNNNNEIAVHDNLTSCGFEIITDFHLFRFRFPFRLVVQQAFTGSNWNPYTNVSLTLD
                     VYGEFWGNKVNRTLGY"
     gene            1619..2158
                     /locus_tag="PK502_01490"
     CDS             1619..2158
                     /locus_tag="PK502_01490"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPG99522.1"
                     /translation="MEVAKAALRSEIKRQKELVGAERKMDHARKVFGVVESMEEFKQA
                     KTVLAFWSLPDEIYTHDFIMKWAGLKHIALPVMVGDYLELRLFSSKESLVKSSNFSIF
                     EPSKNKLVEPDQVDFAIIPGVAFDMQGNRLGRGKGYYDKLLPKLNKAIKVGVGYNFQL
                     VGQVPVAEHDVMVDRVVCM"
     gene            2189..3589
                     /locus_tag="PK502_01495"
     CDS             2189..3589
                     /locus_tag="PK502_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPG99523.1"
                     /translation="MMTKMKMTLISLFRGNYYGVKSIINRTFLCFLLVLATEVTQGQI
                     PNGYYSNANGKTGSELKTALFNIIKNHTSISYDQLWTAFKSTDKKPNGKVWDMYSDNP
                     GGTPPYEFTFVTDQCGNYSVEGDCYNREHSFPKSWFNDGTPMYSDLFHLYPTDGKVNG
                     QRSNYPYGEVSNPTWTSLNGSKLGPSSYPGYTGVVFEPIDDYKGDFARSYFYMVTRYE
                     DKVDSWSTDMLNGTAYPAFSTWAKNMLLEWNAQDPVSQKEIDRNNVVYNQYQHNRNPF
                     IDHPEYAQLIWGSYTPVSFTSTPITEAQVGTVYTYNITATGTNGVSLTITSTAKPSWA
                     TLTTTGNGAATLSGTPGAANVGSNGVTLTVSDGTTSASQTFTINVSPASIATTSDLPV
                     FRVYPNPVSDVLHIEASEVKMFSVEIFDLIGKTIYTKDKIMEPVEVEMANFPKGIYMV
                     KILANGKTYMVKVVKR"
     gene            complement(3671..4372)
                     /locus_tag="PK502_01500"
     CDS             complement(3671..4372)
                     /locus_tag="PK502_01500"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPG99524.1"
                     /translation="MRKLIFATFLVGLGTMVQAQTSWLNTKVSEMSFYTLPFEYESLE
                     PIIDKATVEIHYSKHQRSYFDKLISAAKGTEYYNKTLAEIFEEINSVPAAIRNNAGGY
                     FNHILYWDVLKPGNRSNTSKELLNAITLSFGSTDELKRQVTQEATNFFGSGWVWLCAD
                     RNGGLFVCSTPNQDNPLMANAQKIGTPILAIDLWEHSYYLKYQNRRADYVNQLWDAIN
                     WEVVSELYKQTNSKK"
     gene            4554..4958
                     /locus_tag="PK502_01505"
     CDS             4554..4958
                     /locus_tag="PK502_01505"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPG99525.1"
                     /translation="MAKLNENELTANNINLVGLGTEISGDISSNGDLRIDGTLIGNLS
                     VKGKVVIGETGRIKGELVCKNSDISGQVEGKITVQELLSLKASARVNGDLNIGKLAIE
                     PGCKFTGYCNMLSAEQNFRSDSKLDSGTSLGK"
     gene            5363..6481
                     /gene="atpB"
                     /locus_tag="PK502_01510"
     CDS             5363..6481
                     /gene="atpB"
                     /locus_tag="PK502_01510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPG99526.1"
                     /translation="MTSFISRYIISAALVVGLVVGVEEVRADDIAGPVETDSTQTEKS
                     KGFNVADFIFEHVGDSYEWHILTVNGHHISIPLPIILYSRTSGLQVFLSNKFHNSTRT
                     YKGFKWEDEGKYAGKIVQLNSAGEVDDANPLPLNFSITKNVLTIFMLGIFLCWLFISI
                     AKQYKKRPNSAPTGIQNMLEPIVLFVRDEIALPSLGEKQYQRFMPFLLTLFFFIWFAN
                     MLGLIPIFPGGANVTGNIAVTMVLAVFTFVVITVNGNKHYWKDIFNAPGVPWFLKLPI
                     PIMPLVEIMGMFTKPIVLMVRLFANILAGHMVGIVFMSLIFIFAGMKFWLGYVAAPIS
                     LIFSIFMTALELLVALIQAYVFTLLSAIYIGMATTEHE"
     gene            6513..6770
                     /gene="atpE"
                     /locus_tag="PK502_01515"
     CDS             6513..6770
                     /gene="atpE"
                     /locus_tag="PK502_01515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPG99527.1"
                     /translation="MTTLLILLQAAQGAALGKIGAAIGAGLAVIGAGMGIGSIGKSAL
                     EAIARQPEAAGDIRSNMIVAAALIEGVAFFALIVCILVIFL"
     gene            6783..7277
                     /gene="atpF"
                     /locus_tag="PK502_01520"
     CDS             6783..7277
                     /gene="atpF"
                     /locus_tag="PK502_01520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPG99528.1"
                     /translation="MGLVTPDYGLLFWMLLSFSIILYILKKFAWKPIIHALKNREDSI
                     ALSLKQAEQARAEIVKLQEENKALAEKARLEREKEFQETKALRDKLLIEAKEQARLET
                     QKMLDKAREQILSEKEAAKKELHSTIVSMSVEISEKVLRKQLAEKDRQVEYINELLNE
                     MSRN"
     gene            7292..7828
                     /gene="atpH"
                     /locus_tag="PK502_01525"
     CDS             7292..7828
                     /gene="atpH"
                     /locus_tag="PK502_01525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HPG99529.1"
                     /translation="MSSGRVATRYAKGLLKHGNQKGNSPEIFASVQFMIRILDEVKML
                     REFIDNPAFSTDEKCNKIKELVGDQKHTDIVNFIQLLLKKHREEYLYNSLHIFRNLYL
                     RENNILDVVIESVNELDDNVVSRIEAYISSQFGKKCEIKVEVNPALIAGIVILVDGKV
                     IDYSVKGQLEGFRKKLVG"
     gene            7865..9445
                     /gene="atpA"
                     /locus_tag="PK502_01530"
     CDS             7865..9445
                     /gene="atpA"
                     /locus_tag="PK502_01530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPG99530.1"
                     /translation="MADIKPSEISELIKDELADLKYKPKFEEVGFVLQVSDGIARVFG
                     LRNVKSNELVEFESGVKGIALNLEEDNVGVVLLGDTFKVKEGDKVKSLGEIASINVGE
                     GLLGRIINTLGEPIDGKGPIAGKTIQLTLERKAPGVIYRQPVSQPLQTGLKAIDSMIP
                     IGRGQRELIIGDRQTGKTAIAIDTIINQRNNFLKGDPVYCIYVAIGQKGSTVASLART
                     LEEHGAMEYTTIVVATASDPAAMQFYAPFAGATIGEYFRDTGRHALVVYDDLSKQAVS
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKIINNDSIAANMNDLPEPLRPFVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESNLFNNGIRPAINVGISVSRVG
                     GNAQIRSMRKVAGTLKLDQAQFRELEAFAKFGADLDAATISVLEKGSRNVEILKQGQF
                     KPLPVEEQVAIIFCGTAGLLKNIPINRIADFEKDFLERCELTLKEEMKRIAAGEFTDD
                     IAKALTQLVSSVSKNYNA"
     gene            9584..10456
                     /gene="atpG"
                     /locus_tag="PK502_01535"
     CDS             9584..10456
                     /gene="atpG"
                     /locus_tag="PK502_01535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPG99531.1"
                     /translation="MANLKEIRTRISSVTSTRKITGAMKMVSAAKFKKAQNNLLRFRP
                     FYEKINHLAADLIKSSVSNESAYMIEREQVRNIGVVLITSNNSMCGAFNANIIKRALV
                     VLNDLNRQYPQAKVIFYPLGKTGEEFLVKNKFNVVSCNNAIIEKPSVERTKDFFNLLN
                     SLYLGNELDRIMLVYNSFKNAATQIQKVETLLPLVTPDEQYGSEDLRIVEPDPATLLD
                     RLLPYYLLNSLHSSILESSAAEHGARMTAMHQATENATTIMNNLILEYNKARQASITN
                     EILEIVSGANALSH"
     gene            10610..11170
                     /locus_tag="PK502_01540"
     CDS             10610..11170
                     /locus_tag="PK502_01540"
                     /inference="COORDINATES: protein motif:HMM:NF023238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 domain-containing protein"
                     /protein_id="HPG99532.1"
                     /translation="MKKTTMFISIFLVMIMGAVQSSCIGSFKLSKKVLTWNKSLGGKF
                     LNELVYILFWVLPVYEVSMLIDGLILNTIEFWTGSNPVAMNPGEVESKIVEQNGVRYQ
                     VVASQNRFDFTQLDGPQKGQSGALVYSPTTKTWSYEGETLSVELLRLNDDGTTSVLLP
                     EGATVAVDATSGLTASLRSFAGVEVL"
     gene            11392..12366
                     /locus_tag="PK502_01545"
     CDS             11392..12366
                     /locus_tag="PK502_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPG99533.1"
                     /translation="MFTADQLSAIFSELLQGYSFEKEPRKLYEPIRYVLSLGGKRIRP
                     ILTLAACNMFSDDIEPAKIPALAIEVFHNFTLIHDDIMDNASIRRGKETVHEHWDTNV
                     AILSGDAMSILAYKLLTQTHLEYLQQVMNIFNSSALGVCEGQQLDMDFESREMISRDE
                     YIKMIELKTAVLLKCALQIGATLGNATTRQVELVGQLGTYLGLSFQLQDDLLDAFGEV
                     GAFGKAIGGDIVAGKNTILPVETTALISPEEAGKFIKILKANNLKESDKISMIMDYYN
                     RVDVKAVVEELIAQYQTKAAEIFEKLDIDNNRKTTMHTIMDSLVGRKK"
     gene            12517..13359
                     /locus_tag="PK502_01550"
     CDS             12517..13359
                     /locus_tag="PK502_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPG99534.1"
                     /translation="METKPQTFVIPWDFTPVAENAFKYAVKLSKVSNEPSQIDLVHVL
                     HSGGSPTKSGKTSEEQVQEMIQADKQRLSTTYSVEINTKLLEGSLFDVITDYASEVHA
                     NMVIMGTHGIKGVQKLTGSWALKVIAGSDVPFLVVQDEPAKDRIFSQLVMSIDFRDEE
                     KEKILWASRMAKQYGSKIHVIVPNSFDSGVKKKISNNLAFAKKQFENVGISWEQHNAA
                     KGKSFQEEIVNLAVDLEADLILIMTAPNLDFTDYVFGAQEQYIIANRSKIPVMCINFG
                     TINP"
     gene            complement(13349..14395)
                     /gene="mnmH"
                     /locus_tag="PK502_01555"
     CDS             complement(13349..14395)
                     /gene="mnmH"
                     /locus_tag="PK502_01555"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008752.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HPG99535.1"
                     /translation="MEVSSIHINSFLTSRESCPVVDVRTPDEFNRGHIPGAVNIPLFT
                     NEEREQVGTKYVRESRYKALLAGLDLAGPKLTSYLHQLKKVASGKNILVYCWRGGLRS
                     SSMAWLFNLAGYNSKTLAGGYKSYRRFAQQFFTNPYRFIVLGGMTGSGKTELLNRLKT
                     INEQVIDLEALANHKGSAFGWIGQPPQPTTEQFENLLFEQLLKLDPLKPIWIEDESSS
                     IGTIFIPPTIYSRMVKCPTIVVDVAKEERVARLVEDYARFSADDLVLSVTRMTKRMGG
                     DRAKQCIDYIRAGNFKEAIDIALSYYDKTYNYGLMSKTAKPITIKTSTKTENSLSLLL
                     AAKNSIGELSWPKD"
     gene            complement(14441..15364)
                     /gene="rsgA"
                     /locus_tag="PK502_01560"
     CDS             complement(14441..15364)
                     /gene="rsgA"
                     /locus_tag="PK502_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPG99536.1"
                     /translation="MFLGVVVKSTGNLYRVKLEDGSTVECTVRGKLRLKGSKTTNPLT
                     VGDRVNVETSHEGTAVIVDILPRTNYIIRRSTNLSRESHIIAANIDQAILVVTIIQPE
                     TQTAFIDRYLVTSEAYSIPAILVFNKVDILNAAQTAMMNQFISIYEEVGYKCLCTSAT
                     TGYNLDAFRQLLQGKTSLISGNSGVGKSSLINAIEPTLQLKTSSISAAHLKGKHTTTF
                     SEMFELSFGGYVIDTPGIKGFGLIDIDKKELYHYFPEIFRLKDKCRFYNCTHVHEPGC
                     AVIEAVESGTISQSRYMSYLSIYFDENSKYR"
     gene            complement(15408..16494)
                     /gene="prfB"
                     /locus_tag="PK502_01565"
     CDS             complement(join(15408..16430,16432..16494))
                     /gene="prfB"
                     /locus_tag="PK502_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132894.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPG99537.1"
                     /translation="MITAEQVKDIVRRRDALRRHLDIDLKQVELDEEEQKTQYPGFWD
                     NPSEAESQLKKIAGIKIWTNGFSKVNSLVEDLLVLYDFAKQGDATDVELEEHFNSTLK
                     SIEELELKNMLRKEEDNLGAIMKINSGAGGTESMDWAAMLMRMYMRWGERNGYDVKVL
                     DIQEGEEAGIKSATLEFTGEMAYGYLKSENGVHRLVRISPFDSNARRHTSFASVFVSP
                     AIDDRIDVVVNQGDIKWDTYRSSGAGGQNVNKVETGVRLYHIPTGIVIENTESRSQFQ
                     NKENAMRLLKSQLYELELRKRREAQAEIEGSKKKIEWGSQVRNYVLHPYKLVKDLRSN
                     YETSDVNAILDGDLNECIKAYLMQYGE"
     gene            complement(16821..17894)
                     /gene="amrB"
                     /locus_tag="PK502_01570"
     CDS             complement(16821..17894)
                     /gene="amrB"
                     /locus_tag="PK502_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPG99538.1"
                     /translation="MKKLSIFAIVLASMVACTSSKSPKSVRFFADTVGFASKATQMDS
                     VYARIFRFQGDSLNRKQALPGARLVICPHDDYAYVGWQYPATLRNITAKTVLILGVAH
                     KAKTFNIENKLVFDSFDCWHGPYGDVKVSPIRDELLRLMPADMVVVHDSLQLAEHSVE
                     PMIPFLQRSNPNIEIVSILVPYMPFARISELANSLAQNIYSISSKKHLEWGKDFAFII
                     STDAVHYGDEGWGGNNYAPFGADSAGYAKAVEHEYQIVDSCLAGKATPERIERFYRYT
                     VSDADYREYRWTWCGRYCVPLGLATAIRLSNLSKGSSLEGKLVSYSTSIDHQPLLVED
                     LGMGRTAPASIRHWVGYASLVYK"
     gene            18130..20013
                     /locus_tag="PK502_01575"
     CDS             18130..20013
                     /locus_tag="PK502_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPG99539.1"
                     /translation="MVKKSNNERLFAEFPPVSTTEWEAAIAKDLKGADYEKKLIWKTN
                     EGFNVKPYYRSEDLADIQTVHTKPGEFPFVRGNNAKKNPWLIRQDMDVCLDKPTEANG
                     KALEWLQRGVDSVGFKLCSDCEPTFDSISRLLKGIDLNCVEVNFTGGVPGAKALPYIL
                     QYLEKGKFDIRQVRLSFDYSPLSNLTLNGKFCCEKDSSYANLQELVENVKSFPNVKVI
                     GINGNIFHNSGSSSVQELGFSLAMANDYIQFLLQKGYGIDDISPRIRFNFAVGTNYFM
                     EIAKFRAARLLWAKMVEVYKPTIETSARMSIHAETSRWSMTVYDPYINMLRTTTESMS
                     AVLAGVDSLNVLPFNAPYSEPTSFSERIARNQQILLREEAYFGKVADPAAGSYYIERL
                     TLSIAEHAWNLFLKVNEMGGYRDAFIKGYVQERISEVAAKRDANIATRRETILGTNQY
                     PNFNEVADLNVVTAEVVTRQNKLKPADAIAQPLTPYRGAQAFEELRYRTDKSGRRPKV
                     FMLTLGNLTMRRARAQFSSNFFAVAGFEILDNIGFNSVDEGVKTALDSQSEIVVLCSS
                     DDEYSELAPIAFEKLAGKTIFVVAGEPQCKSELESKGISNFISIKSNVLETLKGYQKM
                     LSI"
     gene            20173..22308
                     /gene="scpA"
                     /locus_tag="PK502_01580"
     CDS             20173..22308
                     /gene="scpA"
                     /locus_tag="PK502_01580"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HPG99540.1"
                     /translation="MNPDFKKVRINEVNAPKSPLSYNPEWVTSEKIPVMPAFSEADLE
                     SMEHLDYAAGIPPFLRGPYSTMYVMRPWTIRQYAGFSTAEESNAFYRRNLAAGQMGLS
                     VAFDLPTHRGYDSDHERVVGDVGKAGVAIDSVEDMKILFDGIPLNKMSVSMTMNGAVL
                     PIMAFYIVAALEQGATLEQLSGTIQNDILKEFMVRNTYIYPPEFSMRIIADIFAYTSK
                     LMPKFNSISISGYHIQEAGATADIELAYTLADGLEYIRTGLRAGIDIDAFAPRLSFFW
                     GVGMNHFMEIAKMRAARLLWAKIVKQFNPKNPKSMALRTHSQTSGWSLTEQDPYNNVA
                     RTCVEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYLQEETNICKVVDPWA
                     GSYYVEYLTNELAHRAWALIQEVEELGGMSKAIETGIPKMKIEEAAARRQARIDSGKE
                     IIVGLNKYRLDKEDPIEILNVDNTAVREAQIRRLNKLKAERNNEEVRLALEAITRCVE
                     TGKGNLLELSVEAAKRRATLGEISMACEKVVGRYKAVIRSISGVYSAESMEDQNYAKA
                     RELCEKFAKLEGRQPRIMIAKMGQDGHDRGAKVVATGYADIGFDVDIGPLFQTPAEAA
                     KQAVENDVHVLGVSSLAAGHKTLVPQVIEELKKLGRDDIMVIVGGVIPPQDYQFLYDH
                     GVVGIFGPGTPVSVAAVKILEILLERVTD"
     gene            complement(22621..23574)
                     /locus_tag="PK502_01585"
     CDS             complement(22621..23574)
                     /locus_tag="PK502_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPG99541.1"
                     /translation="MENVVAGIDIGGTNTVVGLVTKSGEILKETTLSTRLETTFDGFI
                     KILVSEIEKLLMTFEHDYKLVGIGIGAPNSSYDLGAIVDAPNLQWKGILPVCKEITKH
                     TGVNSVVTNDANAAAVGEMLFGDAQGMKNFVILTLGTGLGSGIVVDGRLVLGSEGFAG
                     EFGHIVAKPNGRQCGCGKRGCLETYASATGIRRTAFKMLADSNHPSLLRNITYDKLTS
                     KFITEAAKTGDPLARSAFEYTGLILGTRLADLVAILNPEAIFFFGGLANAGELLLDPT
                     RRYMEEYLFPVFRGKVKLLISGLQNKNAAVLGAAALMWKEL"
     gene            complement(23750..23983)
                     /locus_tag="PK502_01590"
     CDS             complement(23750..23983)
                     /locus_tag="PK502_01590"
                     /inference="COORDINATES: protein motif:HMM:NF027444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99542.1"
                     /translation="MFAFLTFLFYFFLTLMIIGFLGRLIFRYWMKRVYRKMNGNANET
                     KKRKTTTRQTREKPSKKIINKDQGEYVDFEEID"
     gene            complement(24018..26762)
                     /locus_tag="PK502_01595"
     CDS             complement(24018..26762)
                     /locus_tag="PK502_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99543.1"
                     /translation="MKKVGILITVAALVVGTIAVVVYNWLSNERKKDIEATAALPADA
                     PLVVKIDSYTQISQSLKASNEWKSLLTREDVALVDRMVAITDSMVSVNPVAAGLLKPS
                     AIYAAILPQGDKSNQLLVSLKVPENISISDIRQLIEPLAFGSYTSHVKTYNRTEMLVL
                     TSTSNPQKQAALAIKHGILMVGSREIMVEETINRLESEYTLQSNQKFATINQMAGQGV
                     DASLFINFSKLPVLLTSWLNDNFQANANFVADVGDWGEVDLQLTKSRLTTSGIITTSD
                     SATSFLGLIADQKPVQPEIPGILPAQTSFYFWLGISDLGRYLEDYRTYLDRSGRLFAY
                     TQALAKIGGKVAMEPEELYKRILLNELCLVCLPSTPSGNVDEWYVVAQNQSSSTTGHL
                     LGSLSGKGGSLPAASINVDDKHSYGVFRSPVPGIHRALFGDLFSVVSDEYYCLIDQQI
                     VFGSSIKSLEFFIKEASRGTTLQNSLLHQDVEKELPEKANYRIYTVPSKAKRLLGNYL
                     SNRMATMLTSSPTGKTGALACQLAGGNRNIVATLTVNPSVKEQVAALPVEWVCQLSNT
                     PTLMPQVVVNHNTGEKEVLVQDKANTLYLIDRFGKTIWKRTVDGQVIGNVTQVDIFNN
                     RKLQLAFSTSSKLYVIDRNGKDVQGFPVIFQSRATNPLMVVDYEGNHSYRFFQACSDR
                     KIYVFDSYGKIVPGWMFGSTETIVSERIGYARVAGLDYIIVFDQNRPYILNRKGEERV
                     KPERFFAKNPNSHYALGKDEKGSYFVVTSDTKGLIRFMYLNGKVKDMALRHFTSSHSF
                     GYFDVTSDGQDDFVVADGEYLLATDHLGKKIFSTRFKTKLQPELYYFKLEQIMGIGVR
                     AASNDENYLVDRSGEIVTGFPIEGCTMFSVAKLSNLSKMYNLIVGVKQGAVVCYTLNK
                     "
     gene            27263..27676
                     /locus_tag="PK502_01600"
     CDS             27263..27676
                     /locus_tag="PK502_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99544.1"
                     /translation="MGNFIRELQAPCFAATDQPYELETNLSLFSLNGYYKLNVVKNRL
                     FWTAGLGLGMVHAYWEKPLSYTFSEMEHHNIFGPVANVSLTLNVRITKRVYIEASPLV
                     VVMPTNRVYFSTMDEEHFNNFFAFTFFPFGVKFRL"
     gene            complement(27953..28450)
                     /gene="folK"
                     /locus_tag="PK502_01605"
     CDS             complement(27953..28450)
                     /gene="folK"
                     /locus_tag="PK502_01605"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPG99545.1"
                     /translation="MARVYFTIGGNLGAREKNLEEARNLISARIGTVSKQSAIFESEP
                     WGFSSNDNFLGQVLMVETSLTPAALMLEISYIESKLGREHQGIMYASRTIDIDVLFYD
                     QLVLFTPELIVPHKHLHKRRFVLEPLNEIAPNFIHPLFSMTVHELLSSCPDECKVWRY
                     QPVGA"
     gene            29226..29639
                     /locus_tag="PK502_01610"
     CDS             29226..29639
                     /locus_tag="PK502_01610"
                     /inference="COORDINATES: protein motif:HMM:NF025613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4348 domain-containing protein"
                     /protein_id="HPG99546.1"
                     /translation="MKTIITTIVLIMISICTSFSQRTNEDFNIFFFKFSVDSVFQIER
                     IKFPLEFKTWEDPVEIGGDIVTIQIKKEDWKHDYFFMTENYRPQIFDNFEGELRDTDE
                     RLFQWIGIETGVNVKYFFKRIEGKWFLIKKENLGD"
     gene            29799..31016
                     /locus_tag="PK502_01615"
     CDS             29799..31016
                     /locus_tag="PK502_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99547.1"
                     /translation="MRRILFFILIIFNTYACLGQKKLNNEISSYVHFLQKNSYSAKEY
                     IMTLWDKYDIVIFCERFHGEVTQYNLLLDIIKDERFISNIGNIYTEIGSITIQQDLNI
                     FLQTNYSNEELRKDQLLNIYRNLTFSPYWEKYNFYHFLSSINMLNNTVQESLKLKIHP
                     LSFEFPGWENFKTSTEYKNWFYEQPNFDSIMAVNFIKQYEIQLKNNVRKKALVILNYR
                     HAFSKDVKNEMQGNSIYIPNTARYIFEKYPQKVANVLLNTWTSLPVNNPDIGDPHVPI
                     QGGKWDASFKYIGIKSMGFDFENSPFGYDTFDLWPFSTTELKYKDFFTGFVFYLPLTE
                     HYELYGIPGLVSNDFREELRRRLEIFNPVWNWDTQGQKSEINKTLKQLNRLQKERYYK
                     LNRKLKLIDKWLN"
BASE COUNT         8989 a         6412 c         6814 g         8871 t
ORIGIN      
        1 gctgagtttt tcaagtggat acgaagttca ggagctttcg ttcgatagaa atacccgaat
       61 gctatatgca cttgtagtta cccagcaggg caaggaaatt gtttccgtag ataaaaacaa
      121 atcattcaat gttgtttacg gacctacttt tgatgatgtt aactcgatat acgcctttga
      181 cagcagggtg atttttagct ctacggatgg cgatgtagaa aatatatatc tgcataataa
      241 cttaacagac agcacagtaa aaatttataa ttcaagatat agttctaggt attgttccgt
      301 tactaaaaac catgagctta ttttttctga ctatactccc gatggttata cgataggaac
      361 atcaactatt gatttagata atcagaaaaa tgatttgata agcaataggg taaataatag
      421 ctatccaaat tttacattaa ttggtaaggg ttctgttgtg gttgataccc ttcaaattag
      481 tgattcagta tttagctcaa aaccctacaa gggggcggga acactcttta acgtgcacag
      541 ctggatgcct ttttttgtca acatagacgg gactgaggtt acctcaatta atccgggagt
      601 aatgctactt tctcaaaacc ttaccggaac ccttacttcc agtattggct atggatacgg
      661 aaattctcac ctttttcata ccgatgtaac ctactatgga ttttggccgg ttttgtcggt
      721 gaggtttaac ctatacgacg aggatgcgtt tctttataga gttacagaaa atcctgtttt
      781 tcccaataaa aatagatggg aggcaaagct aaacgtttac ctacccttac agctggccaa
      841 cagcagctac caaacctacc tgcaaccctt ttctgaccta accctgtcga atgattatct
      901 tttttcctcc aacgactcga gctaccatag cggtattggc acggtcaaca gcgggttcta
      961 ctttcatagg ctacggaaca tggcacatcg tgacctacaa ccccgtatgg gtctggtttt
     1021 caggcttaac cttgtaaacg caccctttaa taaaaactcc cttgggtcgt tgcaggcggc
     1081 aaaccttaaa atattcctac ccggattcct ggcgaaccaa ggcttgaaat tctcagctgc
     1141 atggcaaaac caaaacctga aacggtacta tttgggaaac gagattagct ttccccgtgg
     1201 gttttcagaa aatgtatcga aaaaatacct tggagcatca gccgattata tttttccttt
     1261 tataaccccc gacctttcct tgggggcact ggcttacatc aaacgaattt cggtgaacct
     1321 attttacgac tatgcccgta attcctacct atgggtgaac aacaacaatg aaattgctgt
     1381 gcacgataac ctcacctcgt gtggtttcga gatcatcacc gacttccacc tgtttagatt
     1441 tcgattcccg ttccgcctgg ttgttcagca agcctttaca gggagcaatt ggaacccgta
     1501 cacaaatgtt agcttaacat tggatgttta cggagaattt tggggcaaca aagttaatag
     1561 aaccttgggg tactagctta cgtatataca accacaatat caaaagcaac taataaaaat
     1621 ggaagtagca aaagcagcat tacgaagtga aataaaacgg caaaaggaac tagttggtgc
     1681 tgagagaaaa atggatcatg caagaaaggt ttttggtgtc gttgaatcta tggaggagtt
     1741 taagcaggcg aaaacagttc tggcgttctg gtcgcttccc gacgaaatat atacccacga
     1801 ttttattatg aaatgggcag gtttaaagca tattgcgttg cccgttatgg ttggagacta
     1861 tctggagtta aggcttttca gcagtaagga gagtctggta aaaagttcaa atttcagtat
     1921 ctttgaaccc tcaaaaaaca agttggtaga gccagaccag gttgattttg ccattattcc
     1981 tggtgtggca tttgatatgc aaggaaaccg gttgggcagg ggtaagggtt attacgataa
     2041 gcttttaccc aagcttaaca aggctattaa ggtaggcgtt ggttacaact tccagctggt
     2101 ggggcaagtt cccgtcgcag agcatgacgt tatggttgat agggtggtgt gtatgtagtt
     2161 tttctgtaaa tatataagtt ttggtaagat gatgacgaag atgaaaatga cgttaatcag
     2221 cctatttagg gggaattatt acggagtgaa atctattatc aatagaacgt ttctgtgttt
     2281 cctgcttgtg cttgctacgg aggttacaca aggtcaaatt cccaatggtt actactctaa
     2341 cgccaatggg aaaaccggtt ccgagctgaa gaccgcactc ttcaacataa taaagaacca
     2401 cacatccatt tcgtacgacc agctttggac ggcatttaaa agcaccgaca agaagccaaa
     2461 tggcaaggtg tgggacatgt actccgataa tcccgggggt actccaccct acgagtttac
     2521 ctttgttacc gaccaatgcg gaaactacag cgttgaaggg gactgctaca accgggagca
     2581 ttcgtttcct aaaagttggt tcaatgatgg aactcccatg tactctgacc tctttcacct
     2641 ttaccccacc gatggtaagg ttaacgggca aaggagcaat tacccctacg gagaggtatc
     2701 taaccccaca tggacatcgc tcaatggaag caagctgggg ccaagttcat atcccggcta
     2761 cacgggggtt gtttttgagc caatagacga ctacaagggc gactttgccc ggagctactt
     2821 ctacatggtt acgcgctatg aggacaaggt tgacagttgg agcaccgaca tgctgaatgg
     2881 tactgcgtat ccagcatttt caacatgggc caaaaacatg ctgctggaat ggaacgcaca
     2941 ggaccctgtt agccaaaagg agatagacag gaataacgtg gtgtacaacc agtaccagca
     3001 caaccgcaac ccgtttattg atcatcccga atatgcacag ctgatctggg gcagctacac
     3061 accggtttct tttaccagca ctcctattac cgaagcgcag gttgggactg tttatacata
     3121 caatattact gcaaccggta ctaatggagt gtctcttacg attacttcta cagctaaacc
     3181 atcatgggca accctaacaa caactggaaa tggagctgca acactttccg gaacgccagg
     3241 cgctgccaat gtgggttcga acggagtaac cttaactgta agtgatggaa caacttctgc
     3301 aagtcaaaca ttcaccatta atgtttcgcc agcaagcatt gctacaactt ccgatttgcc
     3361 agtatttagg gtttacccga atccagtatc agacgttctg catatagaag catcagaggt
     3421 aaaaatgttc tcggtagaaa tttttgatct gataggtaaa acgatttata ctaaggataa
     3481 aatcatggag cccgttgagg ttgaaatggc caacttcccc aagggtatct acatggtaaa
     3541 aattttggct aatggcaaaa cctacatggt gaaggtggta aagaggtaaa ccactgttat
     3601 tccggttttc tataacaaac atgggggcta tttaaccccc atgtttgtta ttctatcagg
     3661 cattttgcgc ttatttctta gaattggtct gcttgtaaag ttcagaaaca acctcccagt
     3721 taatagcatc ccataattga ttgacataat cagcccttcg attctggtat ttcaggtagt
     3781 acgaatgttc ccaaagatca attgccagaa ttggcgttcc gattttttgg gcatttgcca
     3841 taagggggtt gtcctggttg ggcgttgagc atacaaagag cccgccattc ctatcagcac
     3901 aaagccaaac ccatccgctt ccgaaaaaat tggttgcttc ctgtgttacc tgtcgtttca
     3961 actcgtcagt agaaccaaaa ctaagagtaa tagcattcaa taactcttta ctggtgttac
     4021 tacgatttcc tggttttaga acatcccaat agaggatgtg attaaagtag ccaccagcat
     4081 tgttgcgtat agcagcagga accgagttaa tttcttcgaa aatttcggct agggttttat
     4141 tataatattc cgttccttta gcagccgaaa ttaacttatc gaagtaggag cgctgatgct
     4201 tggagtaatg aatttccaca gtagccttgt caataatggg ttccagcgat tcatattcaa
     4261 agggcaaggt gtaaaaactc atctccgaca ctttagtgtt tagccacgat gtttgggctt
     4321 gaaccatagt tcccaagcca accaaaaagg tagcaaaaat taactttctc atagtaatta
     4381 atttttggta tgctcgttaa acatgtgggc tgaaattaag ttcatgagaa tggtgtacaa
     4441 ttattcaagc cgaaaaaaat gggttaaaca atagtaaaaa tttaacaata gctttgataa
     4501 atttatatat ttgctgcctt aaaggagata tgttgaactc aaaatagttg gaaatggcaa
     4561 aacttaatga aaacgaacta accgccaaca acattaacct ggttggccta ggaaccgaaa
     4621 ttagcggaga tatttccagt aacggcgatt taaggattga cggtacacta atcggaaacc
     4681 tatcggtaaa ggggaaagtg gtaattggcg agacagggag gattaaaggt gagctggttt
     4741 gcaagaactc tgatatttcc ggacaggttg agggtaagat tacagtacag gagttgttat
     4801 cgctgaaggc atcagcacga gtaaacggcg atttaaacat agggaagctg gctattgaac
     4861 ccggctgcaa gtttacaggg tattgcaata tgctttcagc ggagcagaat ttcaggtcag
     4921 atagcaaact tgactccggg acatctttag gtaagtaggc ctactgaaaa atgggtaatc
     4981 cttttcatag gatgatagca caggtggtag taatcacgat agtggtatcg gttattatca
     5041 acctttattt tgtttatatt tacaagcaac cggtcggggt tctttctacg atgcttccat
     5101 ttttcccagc cgttgtaagt ataatttttt acctgcagct agcgcgattc ttttcaaagg
     5161 acagcaaaaa ctttaaccaa aaatatttat tgtttagcac agctaagctt tttatcaatg
     5221 ttgcaatctt tgtagtaatt cttcttgccg gcttgctgaa accggtagta ttaatatcac
     5281 tatatttgac cacatacttt gtgctcttaa cccatgaggt ggtaatgctt acaaggttga
     5341 ttaaaaaaag gtagatttga aaatgaccag cttcatttca agatatatta ttagcgcagc
     5401 tctggtagtt gggttggttg tcggggttga agaagttcgt gcagatgata ttgcaggacc
     5461 tgttgaaacg gattctaccc aaacggagaa atcgaagggt tttaatgtag ccgatttcat
     5521 ttttgagcat gttggcgatt cttacgagtg gcatattctt actgtcaatg gacatcatat
     5581 aagcattcct cttcctataa tactttatag cagaactagc ggattgcagg ttttcttatc
     5641 gaacaaattc cataattcca cccgaaccta taaaggcttt aagtgggaag acgagggcaa
     5701 gtatgctgga aaaattgttc agctcaacag tgcaggtgag gtagatgatg caaatcctct
     5761 gccccttaat ttttcaatta caaaaaatgt gctcaccatc ttcatgcttg gcatttttct
     5821 ctgctggtta tttatttcta tcgccaaaca gtataaaaaa aggcctaatt cggctccaac
     5881 aggcatacaa aatatgctgg aacccattgt gctatttgtt cgtgacgaaa tcgccttacc
     5941 ctcgttaggt gaaaagcagt accagcgatt catgcccttc ctgctaaccc tattcttttt
     6001 catttggttt gccaacatgc tgggtcttat tccaatattt cccggaggag ctaacgtaac
     6061 aggtaatatt gccgttacca tggtgttggc cgtttttacc tttgtggtaa ttacagtaaa
     6121 tggaaacaag cattactgga aagatatctt taatgcacca ggagttccgt ggttcttaaa
     6181 gcttcccatt cccatcatgc cgcttgttga aattatgggt atgtttacca agcctattgt
     6241 gctgatggtc cgactattcg ccaacatttt ggcaggtcat atggttggaa ttgtttttat
     6301 gagcctaatc tttatctttg caggaatgaa attttggtta gggtatgtag cagcacccat
     6361 ttcgcttatt ttttctatat ttatgactgc tcttgagctg ctggtcgctc taatccaagc
     6421 gtatgtattc accttgctgt cagccatata tataggaatg gccacaacag aacacgaata
     6481 gctaactaac attttttata aatcttataa ctatgacaac attactaatt cttttacaag
     6541 ctgcccaggg tgcagcatta gggaaaattg gagccgctat tggggcaggg cttgctgtta
     6601 taggtgcagg catgggaatt ggtagcatcg gaaaatctgc gctggaagca attgcccgcc
     6661 aacccgaagc tgccggagat atccgttcaa acatgattgt tgccgctgcg cttattgaag
     6721 gggtggcatt ttttgccctc attgtttgta ttctggttat attcctttaa aatttgtaag
     6781 ctatgggctt ggtaaccccc gattacggac tgcttttctg gatgcttcta tccttttcca
     6841 taattctcta cattcttaaa aagtttgcat ggaaaccaat aattcatgct cttaagaata
     6901 gggaggattc cattgctctt tcgcttaagc aggcagagca agccagagca gaaattgtaa
     6961 aacttcagga agaaaacaag gctttggcag aaaaggcaag attggagcgg gaaaaggagt
     7021 ttcaggaaac aaaagccctt cgcgacaagt tgctcatcga agccaaagaa caggctcggc
     7081 tcgaaaccca gaaaatgtta gataaagccc gcgaacaaat acttagtgaa aaagaagcag
     7141 ccaaaaaaga gctccactcc actatcgtct ccatgtcggt ggagatttcg gagaaagttc
     7201 ttagaaaaca gcttgccgag aaagaccggc aggtggagta cattaatgag ctgcttaacg
     7261 agatgtcgag gaattgattg ctttgtagat tatgagtagc ggaagggttg caaccagata
     7321 tgccaaggga ttgctaaagc atggcaacca aaagggaaat tcgcccgaaa tatttgccag
     7381 cgtccagttc atgattagga ttctggatga ggttaaaatg ctcagggaat ttatagacaa
     7441 ccctgctttt agcaccgatg agaaatgtaa taaaattaag gagctggttg gggaccaaaa
     7501 acatacagat atagttaact ttatccagct gctgcttaaa aagcatcgtg aagaatatct
     7561 ttacaactcg ttacatatat ttcgaaacct ttacctccgc gaaaacaaca tcctggatgt
     7621 ggttatagaa tcggttaatg agttggacga taacgttgtc agtaggattg aagcttacat
     7681 aagttcgcaa tttggcaaaa agtgcgaaat aaaagttgag gttaatccag cattaattgc
     7741 gggcattgta atattggttg atggtaaagt cattgactat agtgtgaaag gacaacttga
     7801 agggtttagg aaaaagttgg ttggatagtg aggttatact ggatgaacac aaacattgat
     7861 tgcaatggca gatattaaac cttcggaaat atcggagtta atcaaagatg aacttgctga
     7921 tttgaagtat aaacccaagt ttgaagaggt tggatttgtg cttcaagtta gcgatggaat
     7981 tgcccgcgtg tttggtttga ggaatgtaaa atcaaacgag ctggtggaat ttgaatcagg
     8041 agttaagggt attgcgctta acctcgaaga ggataacgta ggagttgtgc tgttgggaga
     8101 caccttcaag gtgaaagaag gagataaggt aaagagttta ggagaaattg cctctattaa
     8161 cgtaggagaa ggattgcttg gccgcattat taacaccctt ggtgagccta ttgatggtaa
     8221 gggccctatt gctgggaaaa caatccaact tacactagaa aggaaagcgc caggtgtcat
     8281 ttatagacaa cccgtttctc agccacttca aacaggacta aaggctattg attcgatgat
     8341 tcctattggt aggggacagc gagaacttat aattggtgac cggcaaacgg ggaagactgc
     8401 cattgccatt gacaccatta tcaaccagcg caacaacttc ctaaagggcg atccggttta
     8461 ctgcatttat gtggccattg gccagaaggg gtcaaccgtt gccagtctgg ccagaacgct
     8521 cgaagagcat ggtgcaatgg agtacaccac aattgttgtg gcaacagctt ctgaccctgc
     8581 ggccatgcaa ttctatgcac cttttgccgg tgcaaccatt ggcgagtact tcagagatac
     8641 cggaagacac gccctggtgg tgtatgacga cttgtccaag caagctgttt cataccgtga
     8701 agtttctcta cttcttcgca gaccacccgg tagggaggca tatcctggtg atgtttttta
     8761 tcttcactca cggctgcttg agcgcgctgc aaaaattatc aacaacgact ccatagctgc
     8821 aaacatgaat gacttgccgg aaccgttaag gccttttgtg aaaggtgggg gttcgttaac
     8881 cgctcttcca attatcgaaa cccaggcagg cgacgtgtcg gcatatattc ctacaaatgt
     8941 tatatccata acggacggtc agatttttct ggagtcaaat ttgtttaata atggaattag
     9001 acctgcgatt aatgtgggta tttcggtatc aagggttggt ggaaacgcac aaataaggtc
     9061 aatgaggaaa gttgccggga cactgaaact tgatcaggcg caattcaggg agctggaggc
     9121 atttgccaag ttcggagccg atcttgatgc ggctaccatt tccgtgttgg aaaaaggttc
     9181 gaggaatgtc gagatcctta agcaggggca gtttaaaccc ctaccggttg aggagcaggt
     9241 tgccattatt ttttgcggca cagccggtct tttgaaaaat attcccatta accggatagc
     9301 cgattttgag aaggattttc tggaacgctg tgagctcacc ctaaaggagg aaatgaagag
     9361 aattgccgca ggggagttta ctgacgatat tgctaaggcg cttacccagc tcgtaagctc
     9421 ggtttcaaaa aactataacg cctagccaaa tggactacgg tgccacgtgg agctgttaat
     9481 tttcattgtg gggtaaccat ttacagggtt accccagaat ggccttatta cgaaccgctt
     9541 tacaatgtta tactgctggt tattaaattt agtgtaagta aaaatggcta accttaagga
     9601 gatacgaaca agaatttcat cggtaacttc aacaaggaaa attaccggtg ctatgaaaat
     9661 ggtatcggct gctaaattta aaaaagccca gaataacctg ttgcgcttca ggccgtttta
     9721 tgaaaaaatc aaccatctgg cggcagattt gataaaatca agcgtttcaa acgaatctgc
     9781 ttatatgata gaaagggagc aagtccgtaa catcggagtt gtgcttatta cctccaacaa
     9841 cagtatgtgc ggagcattca atgccaacat tattaaaagg gctttggttg tgctcaatga
     9901 ccttaaccgt caataccccc aagcaaaagt gattttttac cctcttggga aaacaggtga
     9961 agaatttttg gtgaaaaaca aatttaacgt agtttcatgc aataatgcca taattgaaaa
    10021 gccatcggtt gaacgaacca aggatttttt taatttgctt aatagcctct acctaggcaa
    10081 cgagcttgat aggattatgc tggtttacaa tagttttaag aatgcagcaa ctcaaattca
    10141 aaaggttgaa acccttttac ccttagtgac acctgatgag caatatggat ctgaagatct
    10201 tcgaattgtt gagcccgatc cggctactct acttgacagg ttgctgccgt actacctgct
    10261 taactcatta cattcatcca tactcgaaag ttctgccgct gagcatggtg caagaatgac
    10321 agccatgcac caggctaccg aaaatgccac taccattatg aataatctca ttctggagta
    10381 caacaaggca agacaggctt caattactaa cgaaattctc gaaattgtct ccggagcaaa
    10441 cgctttgagc cactaacctc tttgctttat tttggtactg tttggcttgt ataacttcat
    10501 actctgtaat gggggttaaa ttttttttat attctcgtta cataatgatt gcattttatg
    10561 tataaataaa ctaaacttgt taagatgttt aaacctaaat ctttttatca tgaaaaaaac
    10621 aacaatgttt attagcattt tcctggtgat gattatggga gcggtacaat catcctgcat
    10681 cgggagtttt aaactatcca aaaaagtttt aacatggaac aagagtttag ggggtaaatt
    10741 tctaaacgaa cttgtttaca tactgttttg ggtgctacct gtttatgagg tgtcaatgct
    10801 aatagacggg ttaattttaa acaccataga attttggacc ggttcaaacc ccgttgccat
    10861 gaatccgggc gaggttgagt cgaaaattgt agagcaaaat ggtgtaaggt accaagtggt
    10921 tgcaagccag aaccgatttg attttaccca gctggatggc ccacaaaaag ggcaatcggg
    10981 agcgttggtt tacagcccta ctaccaaaac atggagctat gagggggaaa ccctctccgt
    11041 agagctgttg aggctaaatg atgatggaac aactagcgtt ttgcttcctg aaggtgctac
    11101 ggttgctgtt gatgctacat caggacttac ggcatcgctt aggtcgtttg ctggggtaga
    11161 agtactttaa aacagcatgg aattttgtaa agcccgctta ccagctaagc ggttttttta
    11221 gttataaaaa atttggtatt tttatacttc attccaaaca gcctgaagtg ttgttggaaa
    11281 aatataaaaa ggctaagaga gatgctttag tgctggttaa aatcattggt tgtatagttg
    11341 ttggctagga ttttcaggaa gttaaattta ttctaaccct tttatttggt tatgttcacc
    11401 gcagatcagc tgagtgcaat tttttcagag ttactgcaag gttactcctt cgaaaaagaa
    11461 ccccgtaaac tctacgaacc tatacgctat gttttaagtt tgggagggaa acgaatcagg
    11521 cctatactta cgctagcagc atgtaatatg ttctcggacg atattgagcc tgccaaaatt
    11581 cctgcactag ccatagaggt ttttcataac ttcaccttga ttcacgacga tataatggat
    11641 aacgcctcca taagaagggg aaaagaaact gtccacgagc actgggatac caatgttgcc
    11701 attctttcag gagatgcaat gtctattctg gcctacaagc tgcttaccca aacccattta
    11761 gaataccttc agcaagtgat gaacattttc aactcgagtg ctttgggggt ttgcgagggg
    11821 cagcagctcg acatggattt tgaaagcagg gagatgattt ccagagatga atacataaaa
    11881 atgatagagt tgaagaccgc agtgctgtta aagtgcgctc ttcaaattgg agcaaccctg
    11941 ggaaatgcca ctacccgtca agtggaactc gttggacagc ttggcactta cttggggcta
    12001 tcctttcagc ttcaggacga tttgctcgac gcattcggtg aggtaggtgc ttttggtaag
    12061 gcaatcggag gcgatattgt agccggtaaa aacaccatcc tacctgtaga aactactgcg
    12121 ctgatttcgc ccgaagaagc gggtaaattt attaaaatac taaaagccaa caacttgaaa
    12181 gaatccgata aaatatcgat gattatggat tactacaacc gggttgatgt caaagctgtt
    12241 gttgaggagt tgattgccca gtaccaaaca aaagcagctg aaatctttga aaaacttgac
    12301 atagacaaca accgaaaaac aactatgcat accattatgg atagcctggt aggacgaaaa
    12361 aaataattgt gcaaagggtg tgctgctttt attggttatt ttattccaat aattttttgc
    12421 agtaagctta ttacaataaa ttttgttatt acttaaagat gccataaatt tatcataaat
    12481 atttgctaac aaagattata ctaaaccttt agcgaaatgg aaactaagcc tcaaacattc
    12541 gttattccat gggactttac acctgttgcc gagaatgctt ttaagtatgc tgtaaagctc
    12601 tcgaaagtga gcaacgaacc cagccagata gaccttgtgc atgtgctgca ttcaggaggc
    12661 tctccaacca agtcgggcaa gacatctgag gagcaggttc aagaaatgat tcaggcagat
    12721 aaacaacggt taagcaccac ctattcggtt gaaattaaca caaagctact ggaaggaagt
    12781 ctcttcgatg taattacgga ttacgccagc gaggttcatg ccaacatggt cattatgggg
    12841 acccatggta ttaagggtgt gcaaaaacta accggcagct gggcgctaaa ggtaatagcc
    12901 ggttcagatg ttccttttct ggttgttcag gacgaacccg ctaaggatag gattttcagc
    12961 cagctggtga tgtcaattga tttcagggac gaggagaagg aaaaaatctt atgggcttcg
    13021 aggatggcca aacagtatgg ctctaaaatt catgttattg tccccaacag cttcgactcg
    13081 ggagttaaaa agaagattag caataacctt gcttttgcca agaaacaatt tgaaaatgta
    13141 ggcattagct gggagcagca taatgctgcc aaggggaaaa gttttcagga ggaaattgtg
    13201 aaccttgcag ttgaccttga agcagattta attctaataa tgacagctcc taatctggat
    13261 tttaccgatt acgtgtttgg ggctcaggag caatacatca tagccaaccg atcaaagatt
    13321 cctgtaatgt gcattaactt tgggactatt aatccttagg ccagctcaac tccccaatgg
    13381 aatttttagc agcaagcagg aggcttaagc tgttttcggt tttggtcgaa gttttaatgg
    13441 ttataggctt ggcagttttg ctcattaacc cataattata ggttttatca tagtatgata
    13501 gggcaatgtc tattgcctct ttaaagttac ccgccctgat gtagtcaatg cattgtttag
    13561 ccctgtcgcc acccattctt tttgtcattc tggttaccga aagcaccagg tcatctgctg
    13621 aaaatcgggc ataatcttca accagccggg caacccgttc ttctttagcc acatcaacaa
    13681 caattgttgg gcattttacc attcgggagt aaatggtcgg ggggataaaa atggtaccta
    13741 tgctgctgct ttcgtcttca atccatatag gtttcaaggg atctagcttt aggagttgct
    13801 cgaaaagtaa attctcaaat tgctcagtgg tgggctgggg tggttgacca atccatccga
    13861 atgctgagcc cttatggttg gcgagagctt ccaggtcaat aacctgttcg tttatggtct
    13921 taagccgatt cagcagttct gttttgccgc taccggtcat cccacctaaa acaataaatc
    13981 ggtatggatt agtgaagaac tgttgcgcaa acctacggta cgacttgtat cctcctgcca
    14041 gcgttttgga gttataccca gcgaggttga aaagccatgc catgctggaa ctacgtagcc
    14101 cgccccgcca gcagtaaacc agaatatttt taccggaggc gactttctta agctgatgca
    14161 ggtatgaggt gagcttgggt ccagctaaat caaggccggc cagtagagcc ttatagcggc
    14221 tctccctgac gtattttgtt ccaacctgtt cccgctcctc gttggtaaaa agagggatgt
    14281 ttactgcacc gggaatatga cctcggttaa actcatcggg agttctaaca tcaaccacag
    14341 ggcaactttc cctggaggtt aaaaaggagt tgatatgaat tgaacttact tccaatgtgt
    14401 ggcaaagtgt ttgatattgt tttttgaaac aatatagctg ctacctgtat tttgagtttt
    14461 cgtcaaagta aatgcttaga tagctcatgt atcgcgattg gcttattgtt cctgattcaa
    14521 ctgcttctat caccgcgcat ccaggctcat gcacatgtgt gcagttgtag aaacggcact
    14581 tatcttttaa cctgaaaatt tcggggaagt agtggtaaag ctccttttta tctatatcta
    14641 taaggccaaa acccttaatt ccgggggtgt ctatcacata tccgccaaaa ctaagctcaa
    14701 acatttcgct gaaggtggtg gtgtgcttgc ccttaagatg ggctgcggat attgagctgg
    14761 tttttaactg gagtgtcggt tcaatggcgt tgatcaggct tgattttccc acacccgaat
    14821 ttccggaaat caagctggtt ttaccctgaa gaagctgcct gaatgcgtca aggttgtaac
    14881 cggttgtggc tgaggtgcac aggcatttgt aacctacttc ttcataaatc gaaataaact
    14941 ggttcatcat tgcagtttga gcagcgttga gaatgtcaac tttattgaaa actagaattg
    15001 cggggattga gtatgcctct gatgtaacca ggtagcgatc aataaaagcg gtttgtgttt
    15061 cgggttgtat aatggttaca accaggattg cctggtcgat gtttgcagca atgatatgcg
    15121 actcccttga aaggttggtt gaacggcgaa tgatgtagtt tgttctgggc aggatgtcaa
    15181 caattacggc agtaccttca tggcttgttt ccacattcac ccgatcgcct accgttagcg
    15241 ggttggtcgt tttagaccct tttagccgga gcttgcccct aacagtacat tcaaccgtgc
    15301 tgccatcttc gagcttgacc cggtataaat ttcctgttga ttttactaca acacccaaga
    15361 acatagtctg aacaataaat tatttcacta tgggcactac tttctcccta ttctccgtac
    15421 tgcatcaggt aagctttgat gcattcgttt aaatcgccat ccagaatggc atttacatcg
    15481 gatgtctcgt agtttgagcg gagatcttta accagcttat agggatgtag aacgtaattt
    15541 cttacctgcg aaccccattc aatttttttc ttactcccct cgatttccgc ttgtgcttcc
    15601 ctacgcttcc ttagctcaag ctcgtaaagc tgggatttga gcaaccgcat ggcattttcc
    15661 ttgttttgga actgggaacg ggattccgtg ttttcaatca ctattcctgt ggggatatgg
    15721 taaagtctaa cacctgtttc taccttgttt acattctgac cacccgcacc cgacgagcgg
    15781 taagtatccc attttatatc gccctggtta actacaacgt ctatcctatc gtcaatcgca
    15841 ggagaaacaa aaacagaagc aaacgatgtg tggcgacggg cattgctgtc gaatggggaa
    15901 atcctaacca accggtgaac gccattttca cttttcaggt atccataggc catttcacct
    15961 gtaaattcca gagtagccga ttttattccg gcttcttcgc cttcctggat atccagcacc
    16021 ttaacatcat aaccatttcg ttctccccat cgcatgtaca ttcgcatcag catggcagcc
    16081 cagtccatgc tttcggttcc cccggctcct gaatttattt tcattatagc ccccaggttg
    16141 tcttcctcct tacgtagcat atttttgagt tcaagctcct caatgctttt cagggtagaa
    16201 ttgaagtgct cttccagctc cacgtcggta gcgtcgccct gtttggcgaa atcgtagaga
    16261 acaagcaaat cctccacaag gctgttaacc ttgctgaatc cgtttgtcca tattttgatg
    16321 cctgcaattt tttttagttg gctttccgcc tctgatgggt tatcccaaaa accgggatat
    16381 tgagttttct gctcttcctc gtccagctct acttgcttca aatcgatgtc aaagatgcct
    16441 ccttagcgca tcccgtcgcc ttactatatc tttaacttgt tcggcagtaa tcatatactt
    16501 ttttctgcaa atatagcata tattgaagca aaggttgaat ggattagtaa tttgaagaag
    16561 gattttccct attgtttttc tgtgagtaag tctcgagtaa atacagtata ttctttatga
    16621 tgccatagag cggagctggt taaatccgat ttcaaaaagt tagggtagcc gaattttgga
    16681 gtaaggctag atctctgcaa aaaaatgagg gttatgcaat tcttccatcg gtgaaataac
    16741 acacttaatc aagcaagtat tttgcctaaa cctaagttta ttagcttatt caaaagaatg
    16801 gctatgcaaa cctccaatgg ctacttgtaa accaacgagg cataacctac ccagtgtctt
    16861 atgcttgcag gagccgttct gcccattccc aaatcttcta ccaatagcgg ctggtggtct
    16921 atgctagttg agtatgaaac caatttcccc tccaggcttg aacctttgct taagttggaa
    16981 agcctaatgg cagttgccag tccaagtgga acgcagtacc tgccgcacca ggtccagcga
    17041 tactccctgt aatcggcatc gcttacggtg tagcgataaa atctttcaat acgttcgggc
    17101 gttgcttttc cggccaagca gctgtcaact atttgatatt catgctctac cgctttggca
    17161 tagccggctg aatctgcccc gaatggggca taattatttc caccccaacc ctcgtcgcca
    17221 taatgaacgg catcggtcga gatgatgaaa gcaaagtctt tcccccattc caagtgcttt
    17281 ttagatgaaa tgctataaat gttttgggct aagctatttg ccagctcgga aattcttgcg
    17341 aagggcatat acggaacaag aatggaaaca atttcgatgt tcgggttact gcgctgcaga
    17401 aatggaatca tgggctctac cgaatgctca gcgagttgaa ggctatcgtg aaccaccacc
    17461 atatcggcag gcattagcct gagaagttca tctctgattg gcgaaacttt aacatctccg
    17521 taggggccat gccagcagtc gaaactgtcg aaaaccagct tattctcaat gttgaaagtt
    17581 ttagctttgt gggcaacgcc aaggattaat actgtttttg cggtaatatt tcttaaggtt
    17641 gctgggtact gccagcctac gtaggcgtaa tcgtcgtgag ggcaaattac aagcctggct
    17701 ccgggcaatg cttgctttct attcagacta tcaccctgaa acctgaaaat cctagcatat
    17761 accgagtcca tctgagttgc ctttgaggca aaaccaaccg tatcggcaaa aaagcgaacc
    17821 gatttaggac ttttgctgct ggtacaagcc accatgctag ctagcacgat agcaaatatc
    17881 gatagcttct tcatacttca accttaaagg taaaattgtg gggaatttca tgagagaaaa
    17941 tagttaaacc ttaaacattt tcaccaggat gcaagtaaat aattttccat taaaacctca
    18001 atagcaatgg ataaaaagaa ctttcaaatt atgcttaaaa gcatggtaaa ggcaaatatg
    18061 ttactatgca gcgttttacc aattcagtaa ttgctaaatt tgcaaatcaa taatccaact
    18121 agtccgatta tggtaaaaaa gagcaacaac gaaaggcttt ttgccgaatt tccacctgtt
    18181 tcaaccactg aatgggaggc ggcaatagcc aaggatctga aaggagcaga ctacgaaaag
    18241 aagcttattt ggaaaacaaa cgaggggttt aatgttaagc cctactaccg ttcagaggat
    18301 ttagcggata ttcaaaccgt tcataccaaa cccggagaat ttcccttcgt taggggaaat
    18361 aatgctaaga aaaatccctg gctaatacgt caggatatgg atgtttgcct cgataagcca
    18421 accgaagcaa acggcaaagc cttggaatgg ctacaacgag gggtggactc ggtaggcttt
    18481 aagctttgca gcgactgcga accaaccttt gactctatta gcaggttgct aaagggaatt
    18541 gacctgaact gcgttgaggt taactttaca gggggagttc ctggagcaaa agcgctgccg
    18601 tacattttgc agtacctcga aaaagggaag tttgatattc gtcaggtacg actctcattc
    18661 gattacagtc ccctttccaa cttaacactc aacggtaagt tttgctgcga aaaggactcc
    18721 agctatgcca acttgcagga gcttgttgaa aacgtcaaaa gtttccccaa tgttaaggtg
    18781 ataggcatca atggaaatat tttccacaat tcaggctcgt cttccgttca ggagttgggt
    18841 ttttcgctgg caatggccaa cgactatatc cagtttctac tccaaaaagg ttatggtata
    18901 gatgatattt cgccacgtat tcgcttcaac tttgctgttg gcaccaacta cttcatggag
    18961 attgccaaat ttagagcggc caggctgctt tgggcaaaaa tggttgaggt ttacaagcca
    19021 actattgaga catctgctcg catgagtatt catgccgaaa caagtcggtg gagcatgacg
    19081 gtttacgacc catacataaa tatgctccgc acaacaacag aaagcatgtc ggccgttctt
    19141 gcaggtgttg attcgctgaa cgttttacca ttcaacgccc cctattccga gccaacctca
    19201 ttttccgaaa gaattgcaag gaatcagcaa attttactac gcgaagaggc ctattttggg
    19261 aaagttgctg accctgcggc ggggagttac tacattgaga ggttaaccct ttccattgcc
    19321 gagcatgcat ggaacctttt ccttaaagtt aacgagatgg gtggctaccg tgatgccttt
    19381 atcaaggggt atgttcagga gcgtattagc gaagttgcgg ctaagcgtga cgccaatata
    19441 gccacccgac gtgaaacaat tttaggcacc aaccagtacc ccaacttcaa cgaggtagcc
    19501 gatttgaacg tagtaacagc ggaggttgtt acccgtcaaa acaagcttaa acctgcagat
    19561 gcaatagccc aaccccttac tccgtaccgg ggagcgcagg ctttcgagga gctacgctac
    19621 agaaccgaca aaagcggaag gcggcccaaa gtctttatgt tgacccttgg caacctcacc
    19681 atgcggcggg caagagccca attcagctct aacttttttg cagttgccgg atttgagatt
    19741 ctggacaaca ttggctttaa ttctgttgac gagggtgtta agacagcact tgattcacaa
    19801 tcagaaatag tggtgctatg cagctccgat gatgagtact ctgaacttgc ccctattgcc
    19861 tttgagaaac ttgccggaaa gacaattttc gtagtggcgg gagaaccgca gtgcaaatcc
    19921 gaactcgagt ctaaagggat tagcaatttc atcagcataa aaagtaatgt gcttgaaacc
    19981 ctgaaaggct accagaagat gctgtcaatc taaaccctgc ttttccggcg attagccatc
    20041 cgttgaaggt ttaaagtgcg ttatttttat atcataatac attaggaatt tctggttgag
    20101 ccttcaggtt ggataatcaa taattgaaaa gtttttaaaa cgaaattggt aaaaagtaaa
    20161 ttcactacag ggatgaaccc agattttaaa aaagttagga ttaatgaggt aaatgcccca
    20221 aaatccccat taagctacaa tccggagtgg gtaacatcgg aaaaaatacc ggttatgccg
    20281 gctttttcag aagccgattt ggaaagtatg gagcatctgg actatgcggc aggtattcca
    20341 ccatttctga gaggcccgta cagcaccatg tacgtgatga gaccctggac catcaggcag
    20401 tatgccggat tttcaacagc tgaagagtcg aacgcttttt atcggagaaa ccttgctgcc
    20461 ggacagatgg gtttatcggt agcattcgac ctgcccaccc accgtggtta cgattccgac
    20521 catgaaaggg tggttggcga tgttggcaag gcgggtgtgg ccattgattc ggtggaggat
    20581 atgaaaattc tcttcgatgg catacccctt aacaaaatgt cggtttccat gaccatgaat
    20641 ggtgcagtgc tcccaattat ggccttttac attgttgcag cattagaaca gggagcaact
    20701 cttgagcagc tgagcggaac aatacagaat gatatactta aagagttcat ggtgcgcaac
    20761 acctacatct atccgccgga gttctccatg aggattatag ccgatatttt tgcctatacc
    20821 tcgaagctta tgcccaagtt caacagcatt tccatttccg ggtaccacat tcaggaggca
    20881 ggagctaccg ccgacataga gctggcctac acccttgccg atgggctgga gtacatcaga
    20941 acagggctgc gggcaggtat cgatattgat gcttttgctc ccaggctgtc atttttctgg
    21001 ggcgttggca tgaaccattt catggaaatt gccaagatga gggcagcacg tttgctttgg
    21061 gcaaaaattg ttaagcaatt taacccgaaa aatccaaagt ctatggcatt acgaacccat
    21121 agccaaacct cgggatggag cctaaccgag caggaccctt acaacaacgt ggccagaacg
    21181 tgcgtggaag caatggctgc tgcgctaggt catacccaaa gtttacacac caacgccctg
    21241 gatgaggcaa tagcccttcc aaccgatttt tctgctcgca ttgctcgcaa cacccagatt
    21301 taccttcagg aggaaaccaa catttgcaaa gtagttgacc cttgggccgg ttcctactat
    21361 gtggagtatc ttacaaatga actagcccac agggcttggg ctctaataca agaggtagaa
    21421 gagttgggtg gtatgtcgaa ggccattgaa accggcattc caaaaatgaa aattgaagag
    21481 gctgctgccc gtcgccaggc tcgtattgat tcggggaaag agattattgt tggcttaaac
    21541 aagtaccgcc tcgacaagga ggaccccatt gaaattctta acgttgataa tactgcagtt
    21601 agggaggcgc aaatacgccg gttaaacaag ctaaaagccg aacgtaacaa cgaggaggtt
    21661 aggcttgctc ttgaagctat tacccgttgt gttgaaaccg gaaagggaaa cctacttgag
    21721 ctatcggttg aggcggcaaa aagacgcgca acacttggtg aaatctccat ggcttgcgaa
    21781 aaagtggtag gacgctacaa ggctgttatt cgttcaatat caggagttta ctctgctgaa
    21841 tctatggaag atcaaaacta cgctaaagct cgtgaacttt gcgaaaagtt tgccaaactc
    21901 gagggtcgtc agccgcgaat catgattgcc aaaatggggc aagatgggca cgaccgtggt
    21961 gctaaggtgg ttgctacggg ctatgccgat attggatttg atgtggatat tggccccctg
    22021 ttccaaacac cagccgaagc ggccaagcaa gcagttgaaa acgatgtgca cgtgctcgga
    22081 gtgtccagcc tggccgcagg ccacaaaacg cttgttccgc aggtaattga ggagttaaaa
    22141 aaattaggac gtgatgacat catggttatt gttggaggag ttattccacc acaggactac
    22201 caattccttt acgaccatgg agtcgttggt atattcggcc caggcacacc tgtttctgtt
    22261 gctgcggtga aaatacttga aattttactc gagagggtta ccgactaaca gttggaacaa
    22321 tttttaatgt aggaaccaac aacccctagg gctgttggtt ctttttgtgt gagggtaaag
    22381 tgcataagat gaacatgtcc tttcatcatt agaaaattat gtgaatttga agcatcccga
    22441 atggggcttt ataggcatgc cactattttg tggttttcag gatgtttgaa tttgctttta
    22501 atgaagggga atcatagatt ctcttgaaca acctcttttt taccagttca attgcaaaaa
    22561 ttatgctccc gcaggtgtcg aaaagaagcg gctgaaaaaa atcaaaaatt taagcgttat
    22621 ttatagctcc ttccacatca atgcggcggc tcccaggaca gcggcatttt tattctgtag
    22681 ccctgaaatc agaagtttta cctttcccct aaataccgga aacaagtact cttccatgta
    22741 gcgacgtgtt gggtcgagta gcagctcccc tgcattggca agacctccaa agaaaaagat
    22801 agcctcaggg ttaagaatgg ccaccaggtc ggccaagcgg gttccaagaa taagcccggt
    22861 gtattcaaat gccgagcgag ccaacggatc accggttttt gcagcttcgg tgataaattt
    22921 tgaggtcagc ttgtcgtagg taatgtttct caggaggctt ggatggttgc tgtcggccag
    22981 cattttaaat gcggttcggc gaattccggt tgcagaagcg taggtttcga ggcagccacg
    23041 ttttccgcaa ccgcactgcc ttccgttggg tttagctaca atatgcccaa actctcctgc
    23101 aaaaccttca gaacctaaaa ccagtctgcc atcaactaca atgccgcttc ccaaacccgt
    23161 accaagggta agaattacaa aatttttcat accctgtgca tcgccaaaaa gcatttctcc
    23221 aactgctgct gcatttgcat cgtttgttac aacgctgtta acgccagtat gctttgttat
    23281 ctccttacaa acagggagaa tccctttcca ctgaaggttt ggtgcgtcaa caatagcacc
    23341 cagatcgtat gagctgttag gtgctccaat tcctatacct accaacttgt agtcgtgctc
    23401 gaacgtcatg agcagctttt cgatttccga aaccagaatt ttgatgaaac catcaaaagt
    23461 tgtttctagc cgcgtagaaa gggttgtttc cttgagtatt tcaccagact tggtaaccag
    23521 tcctaccacg gtatttgtac cgccaatatc aattcctgct actacatttt ccataatcac
    23581 taataaaatc aattttttta aatgtagaaa gacttacccg ccaacccaca taaaccattc
    23641 ctttagtttc accaatttca cgcaatacgc tcataacgtc gaattactaa taagcagtaa
    23701 gcgtttatta atacaactta agaattacga ttaaattaaa cgggtgaaat tagtctattt
    23761 cttcaaaatc gacatactcc ccctggtcct tattaattat tttttttgag ggtttttccc
    23821 gggtttgcct ggtggttgtt ttacgctttt ttgtttcgtt cgcattgcca ttcatcttac
    23881 gatatactct tttcatccag taccgaaaaa ttaatcggcc caaaaatccg atgatcatca
    23941 gggtgagaaa aaagtagaac aggaaggtta aaaatgcaaa catgatatta ccttttgtaa
    24001 tttttgtgcg aaggtagcta tttatttagg gtgtagcata caacagctcc ctgttttacg
    24061 ccaactatca ggttgtacat tttggacaag ttgctcagct tggcaactga aaacattgta
    24121 cacccctcaa tgggaaagcc tgttactatc tctcccgatc tgtcaaccag gtagttttca
    24181 tcattacttg ccgctctaac accaataccc ataatttgtt caagtttaaa gtagtaaagt
    24241 tcgggttgca atttagtttt gaagcgggtg gagaatattt tctttcccag atggtcggtg
    24301 gcaaggaggt actcaccatc ggcaacaacg aagtcgtctt ggccatcgct ggtaacatca
    24361 aaatatccaa aggaatgcga tgaggtgaaa tgacgcaatg ccatatcttt aaccttaccg
    24421 ttgaggtaca tgaacctgat taagcctttt gtatccgatg taactacgaa gtagcttcct
    24481 ttctcatctt ttcccagggc gtaatggcta ttggggtttt ttgcaaaaaa cctttcgggt
    24541 ttaacccgct cctcgccctt tcggttaagt atatatgggc ggttttggtc aaaaactatg
    24601 atatagtcta gtccggcaac tcttgcgtaa ccaattcgtt cactgactat agtttccgta
    24661 ctaccaaaca tccatccggg aacgattttt ccataggaat cgaatacata aatttttcgg
    24721 tcgctgcaag cctgaaagaa ccggtagcta tggtttccct catagtcaac caccatgagg
    24781 ggattggtgg ctctggattg gaaaatgacc ggaaatcctt gaacatcctt cccattgcgg
    24841 tcaataacgt aaagcttgct gctggtgcta aatgccagct gtagcttgcg gttgttgaaa
    24901 atatcaacct gggtaacatt accaataact tgcccgtcaa cagtacgttt ccagattgtt
    24961 ttcccaaagc ggtcaataag gtaaagcgtg ttggccttgt cctgaacgag cacttccttt
    25021 tcgccggtgt tatggttaac cacaacctgt ggcataaggg ttggtgtgtt gctgagctgg
    25081 caaacccact ccactggtaa tgctgcaact tgttctttta cagagggatt aactgtaagg
    25141 gtagccacaa tatttcggtt accaccggcc agctggcatg ccaatgcccc ggttttacct
    25201 gttggggatg aggttagcat ggtagccatc ctgttgctta aatagttgcc taatagcctt
    25261 ttcgctttag aaggaactgt ataaatacgg tagttcgcct tttccggcag ctccttttcc
    25321 acatcctggt gtaacaagct gttttgaagg gttgttcccc gcgacgcttc cttaatgaaa
    25381 aattccaacg attttataga agagccaaaa acaatttgct ggtctattaa gcagtagtat
    25441 tcatccgata caacactaaa aaggtctcca aatagagccc ggtgtattcc aggcacggga
    25501 gaccggaaaa ctccatacga atgcttgtcg tcaacattaa tgctcgcagc aggcagtgag
    25561 ccgcctttcc ccgaaagact gccaagcagg tgccctgttg tgctggagct ttggttttgt
    25621 gctacaacat accattcatc aacattgccg gatggagtag aggggaggca aaccaaacaa
    25681 agttcattga gcagaatacg cttgtatagc tcttcgggtt ccatcgcaac tttaccacca
    25741 attttggcaa gtgcctgcgt atatgcaaac agccttcctg atcggtcaag gtaggtgcgg
    25801 taatcctcaa gatacctacc aaggtctgaa atgcccagcc agaagtagaa gctggtttgt
    25861 gcgggcagaa tacccggaat ttcgggctga acgggttttt gatcggctat tagtcccaga
    25921 aatgaggttg cagagtcgga tgttgtgatt ataccactgg ttgttagcct actttttgta
    25981 agctggaggt caacctcacc ccaatcccca acatcggcaa caaaatttgc atttgcctga
    26041 aaattgtcgt tcagccagga tgtaagtaga acaggtaatt ttgaaaagtt gataaaaagg
    26101 gaggcatcta ccccctgtcc ggccatttgg tttatagtag caaatttttg gttgctttgt
    26161 agcgtatatt cgctttcaag ccggttaatc gtttcttcaa ccatgatttc ccggctgcca
    26221 accattagga tgccgtgctt gattgctaaa gccgcttgct tctgtggatt cgaggttgat
    26281 gttaatacca gcatttcggt cctattgtag gttttaacat ggctggtata gctgccaaac
    26341 gccaatggct ctataagttg gcgtatgtcg cttatagaga tgttttcggg tacttttaat
    26401 gaaacaagaa gctgatttga cttgtcgcct tgaggcagga tggctgcata aatcgcgctt
    26461 ggttttagca gacctgctgc aaccgggtta acactaacca tgctgtcggt aatggccacc
    26521 atcctatcaa caagggcaac atcctctctt gtcagtagcg acttccattc attgcttgcc
    26581 ttaagcgatt ggctaatctg cgtgtagctg tcaatcttta caactagagg ggcatccgca
    26641 ggtaaagcag cggtggcctc tatatctttt ttacgctcgt tcgacagcca attataaact
    26701 acaacggcaa ttgttccaac cactaacgcc gcaactgtaa ttaaaatgcc aacctttttc
    26761 atctgtcagc tgcttttttt aatgtagcta ctaatggtaa cataaaggtg ctaaattagt
    26821 caggattaat cgtaaaaaga tacttggttt cttagaaaat gcagctcatt ttgctgcggc
    26881 tgatttactc aatcctgtta acccctcgtc ccagctggat ttcttattac atggaattag
    26941 ctgcatgaac atttttctta catttatacc tgttgcggat tatactaatt tgtagcagca
    27001 agagcaaaac ttatccggtt gattgatttt tttaacttac acgatgatgc catatgaagt
    27061 tgacgcataa tagtttaagg ctgatggtgc tgctctcgtc atttgtgata gctggcaacg
    27121 ctaacgcttt atctcttggg agcagcgaca cctgtaaggt tgactattta tcctttcagt
    27181 ctggaatgat aattgaccgc tacaattcgc tgggggtaag gatttttttt gagtaccaac
    27241 gtgatattaa aaaaaaatgg caatggggaa tttcatacga gaactccagg caccttgttt
    27301 tgccgcaacc gatcaacctt acgagttgga aacaaacctc agccttttca gcctgaacgg
    27361 ctactataag ctgaacgttg tcaaaaacag gcttttctgg acagcagggc ttgggcttgg
    27421 aatggttcat gcatactggg aaaagccact aagctataca tttagcgaaa tggagcatca
    27481 taatattttt ggaccggttg ccaatgtaag tcttacgctg aatgttagga ttacaaagag
    27541 agtatatatc gaagcgtccc ctctggttgt tgtaatgcct accaataggg tttacttttc
    27601 gacaatggac gaggagcatt tcaacaactt ttttgccttt acattttttc cctttggggt
    27661 aaagttcagg ctatagagaa acttttaata atgtcataag gttgaaaagg gcaggtcagg
    27721 gtatctccaa ccgtaaacag aaaccaggtt gggcagctgc ctcaaagctg acaaaatgaa
    27781 tagttcgggg tggaaagttt aaaatatggt tttacctctt gggccaacac caaaggttat
    27841 tcaaaacgga ggaatgcgcc aacaatggcg attaacgtaa agggattttt ttaccttggt
    27901 aattactgct acaaccagct taaaccctaa ctttaactta ttatgcttgt agctaggcac
    27961 ctacaggttg gtagcgccag accttgcatt cgtcggggca ggatgaaaga agctcgtgca
    28021 ctgtcatgga aaagagcgga tgaatgaaat tcggagcaat ctcgttgagc ggttcaagca
    28081 caaagcgacg cttgtgcagg tgcttgtggg gaacaataag ttcaggtgtg aaaagcacaa
    28141 gctggtcgta gaaaagaaca tcaatatcta ttgttctgga ggcatacatg attccctgat
    28201 gctcacgtcc cagcttcgac tctatgtagg atatctcgag catgagcgca gctggggtta
    28261 agctggtttc gaccatgagc acttgcccta gaaaattatc gttgcttgag aaaccccaag
    28321 gttcagactc aaaaattgcc gattgcttgc taactgtccc gattcgtgca gaaataaggt
    28381 tgcgtgcttc ttccaaattt ttctccctgg cacctagatt cccgccaatt gtgaagtaaa
    28441 cccttgccat ttccctttac cagtttatat ggtacaaact tatttgaaat tttactaaaa
    28501 tgttcttaga ccggttaatt tttacgaaca attttaatgg tatgtttggt tttcgtcgaa
    28561 tagctatatt tttaatttac aagaacccat tattacaaca gcaaaaagaa taccagctgc
    28621 aaaggttaaa caggcgaatt cggtgtaatg ttcacctaac acatctactg ccttaaaagt
    28681 tggttatggc cattttaacc ttttaccggc aaagtttgct acatagccgt agctaagtat
    28741 ggcctcctgc ctgcaatcaa acagcaaatt atcccttact gcaaggctgt taaagggctg
    28801 ctcttttgca acgatgtagc aaccccatgc tcaaatacag gcgcccaaag taacaccaat
    28861 cgagggttac gctacgctga ccgatccgcc agctagggga gagctcgccg aaggtgacct
    28921 ataccaagcg agggaataga tttaaacttt attcagaaac ttttcgggca caaacattct
    28981 aaaaccacag aaatttacat gcacgttact gaaaaaaact acaaaaaatt aaaccgcctt
    29041 ttgatggttt gtagtaagga agttcaaaag tttggtttat caaataaata atttttctac
    29101 ttttgaatag aaatagaacg caactttatg gcgtctatcc atccaaatat ggaaataagg
    29161 cgcaacagag tggcgtgtat aaatgagttg ggcacaatgc tgagattctt gttacataac
    29221 attttatgaa aactattatt accacaattg tcttaatcat gatttccatt tgtacttcct
    29281 tttctcaaag gacaaatgaa gactttaata ttttcttctt taagttttct gttgactcag
    29341 tttttcaaat tgaacgaata aaatttcctc ttgaattcaa gacttgggaa gatccagtag
    29401 agattggagg agatatagtg actattcaaa taaaaaaaga ggattggaaa catgactatt
    29461 tttttatgac tgaaaattat cgtccacaga tctttgataa tttcgaagga gaattaaggg
    29521 atactgacga aagattattt caatggatcg gaatagaaac tggagttaac gttaaatatt
    29581 tttttaaaag aatcgaagga aaatggtttt tgataaaaaa agaaaatctt ggtgattaac
    29641 caagcactgt gcccaacacc gggtaaattc cattgccggc aaagtgctct tgcgggtttc
    29701 aactcatttg ggttcgtttg gtgtacccgg acaagtgggc gctccgaacc aggcaacggg
    29761 ctatacccga aaacgttagc aaccataatg ataaaagaat gagaagaata ctatttttta
    29821 tactcattat ttttaataca tacgcctgtc taggacaaaa aaaattaaat aatgaaattt
    29881 catcctacgt tcacttctta caaaagaatt cttattctgc caaagagtat ataatgactc
    29941 tttgggacaa atatgatata gtaatttttt gtgaaagatt tcatggagaa gtaacgcaat
    30001 ataatttatt gctggacatt attaaagatg agcgctttat atcaaatatt ggtaatattt
    30061 acacagaaat tgggagcata acaattcaac aggatttaaa tattttttta caaaccaatt
    30121 attcaaatga agaactaaga aaagaccaac tgctcaacat ttataggaat ttaacgtttt
    30181 ctccctattg ggagaaatat aatttttatc actttctctc atcaataaac atgttaaaca
    30241 atacggttca agaatcactt aaactaaaaa tacatccatt atcctttgag tttcccggtt
    30301 gggaaaattt taagacctcc acggaataca aaaattggtt ttatgaacaa ccaaattttg
    30361 attcaattat ggcggtcaat tttattaaac aatatgagat acaattaaaa aacaatgtac
    30421 gaaaaaaagc tttagtaatc ctgaactaca ggcatgcatt tagtaaagat gttaaaaatg
    30481 aaatgcaagg aaattcaatc tacataccta atacggcaag gtatattttt gagaaatacc
    30541 cacaaaaagt agcaaacgta ctactgaaca catggacatc cttgcccgtt aataatccag
    30601 atattggtga cccacatgtt ccaatacagg gaggtaagtg ggacgcctca ttcaaatata
    30661 ttggaattaa aagtatgggg tttgacttcg aaaattcacc atttggttat gacacatttg
    30721 acctttggcc tttttcaaca accgaactaa aatataaaga cttttttact ggttttgttt
    30781 tttatttgcc cttaacagaa cattacgaat tatatggaat tcctggccta gtttctaatg
    30841 attttcgaga agaattaaga cgaagattag agatttttaa tcctgtgtgg aattgggaca
    30901 cacaaggaca aaaatccgaa attaataaaa cactaaagca attaaatagg ttacaaaaag
    30961 aaagatacta taaactaaac cgaaaattaa aattgattga caaatggtta aattaaaata
    31021 gatttctgtt gttaacaggt ggtttagcct actggagctg atttgcagat tagaaagttt
    31081 ttagta
//
LOCUS       DAOPHX010000010        30785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_1430_length_30785_cov_5.670387, whole genome shotgun sequence.
ACCESSION   DAOPHX010000010 DAOPHX010000000
VERSION     DAOPHX010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 30785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30785
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1430_length_30785_cov_5.670387"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..156
                     /locus_tag="PK502_01620"
     tRNA            84..156
                     /locus_tag="PK502_01620"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:117..119,aa:Gly,seq:gcc)
     gene            218..302
                     /locus_tag="PK502_01625"
     tRNA            218..302
                     /locus_tag="PK502_01625"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:252..254,aa:Leu,seq:taa)
     gene            450..1814
                     /locus_tag="PK502_01630"
     CDS             450..1814
                     /locus_tag="PK502_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPG99548.1"
                     /translation="MRVHFIAIGGSAMHNLAIALKQKGYEVTGSDDELFEPSLSRLKQ
                     HGLLPSEIGWYPERLTPDIDAVVLGMHASSENPELQKARVLGLKIYSYPEYLYEQTRG
                     KTRVVIGGSHGKTTITSMVMHVLQQAGFDFDYMVGAQLEGFDNMVGLNNQTNLAVFEG
                     DEYLTSPVDPRPKFHLYKPTIAVISGIAWDHFNVFPTIENYRDQFVGFISCIEPNGTL
                     VYCSEDDEVAGVVSSTKRSDLELIPYTTHPYRIEPNVGCQLVTEQACYPLKIFGRHNM
                     QNISAAKAVCLKLGVSESNFYRAISTFKGAAKRLQRLAEGANSTVFLDFAHSPSKVAA
                     TIGAVLEEYPNRKLVACLELHTFSSLNINFMSQYRNVFSGVAVPIVYFDPVTVEHKNL
                     PPLNINEVRDAFGQPIPDVCISASEMVEKLNGMNWENANLLLMTSGNFSGLNLNGLAL
                     QIAR"
     gene            1897..2376
                     /locus_tag="PK502_01635"
     CDS             1897..2376
                     /locus_tag="PK502_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99549.1"
                     /translation="MKIARVIVVSLALIMAVTTARAQDYKTGLGLRGGYPSGITVKHF
                     FGSKTAVDGILSFGWGGIGITGLYELHNRIPSAPGLNWYYGFGAHFATADANKNNPFA
                     NDRGDDVFLGVDGAIGLEFTFPDAPICISLDILPILNLIESPGIWFNAGLSVRYAFK"
     gene            2470..2541
                     /locus_tag="PK502_01640"
     tRNA            2470..2541
                     /locus_tag="PK502_01640"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2503..2505,aa:Glu,seq:ttc)
     gene            2570..2824
                     /gene="rpsT"
                     /locus_tag="PK502_01645"
     CDS             2570..2824
                     /gene="rpsT"
                     /locus_tag="PK502_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPG99550.1"
                     /translation="MANHKSAEKRIRETETRRLHNRYYAKTTRNALKALRNTTNKAEA
                     IEMLPKVSSMLDKLAKKHIIHRNKAANLKSSIQKHVNALQ"
     gene            complement(2881..3687)
                     /locus_tag="PK502_01650"
     CDS             complement(2881..3687)
                     /locus_tag="PK502_01650"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4163 domain-containing protein"
                     /protein_id="HPG99551.1"
                     /translation="MLKSRIIRLLSLAVLMGLVYFLFNTVRATYTPNLSVAPDSLVFT
                     RGCVEPDSSCFRILLKFPVVQGKRAARIQKMIAYDYLKLLSDSTKVKGVNQFKEVLTR
                     LTESYDSAYVDYLSLFPGSNGISWYLNADYDVLLNDGKVLTIQYRYEDYLGGAHGMYG
                     YHYQNIDLRKSRPLSLNDIVSNTKQFSELAEKLFKGRVGVDHLNGYWFEGGKFSLPRE
                     FGLSENGIVLHYNVYEIASFADGDLVFEIPYSSMGGMLSKRFRYLAEEGK"
     gene            4062..9608
                     /locus_tag="PK502_01655"
     CDS             4062..9608
                     /locus_tag="PK502_01655"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HPG99552.1"
                     /translation="MNSKSIKWLLTVGVIAIIILQVGCKGKQDVEISTDPLFGEYISA
                     YTSGMISAHSAITIKLTKPSSKFTFNGDEVHEGYFSFSPSVKGKAYWVDDQTLEYRPT
                     EVMRSGKVYSSSLALSKLFKGVEPKLKTFRFSFRVIPQSVSIELNGLIVRDAENPNSY
                     ELDGTVQTADVAESEKLNECIKASVNGMSIATKLDSTEVVNTYHLRLEGIERTVKKGE
                     VRISWNTSAIGGNSKGYEILEVPPLGSFKVISAKAIQTPEQSVTIIFSDLLDRNQNFE
                     GLVELEGISGLRYEVDRNMLRVYPPETIEGEKNLNISAGVLNAIGKRLNAEFSQNLLF
                     EDVNPALRTVGHGAILPTTQGLIFSFEAVNLKAVSVQIVKIYESNVPYYLQVNTMGGT
                     NELRRVGYPVLNKVIGLNQFGPIVPNKWTRYTLDLAQLFKADRGAIYQISITFNKKQL
                     LSSCGDDSDQTTPTSPFSPGDEEVARGYDGPGYYDDYYYYYDDDYNWRERDNPCNSAY
                     YSESRMINQIIISSDIGIIAKLGNDGGMLVAVTDLPSAKPLSNANVRISDYQNQTIAD
                     GNTNNDGFFEFKTTRRPFLVTASDGKQKGYLRVDNGTSNPLSNFDVSGNEVQHGLKGM
                     IYGERGVWRPGDTLHLCFILEDKGKTLPPNHPVVLELHDPKGLLVRRAVQPNNDAGIF
                     YFPTVTDPQAPTGPWSATVRVGGAIFQKTLKVESIKPNRLKINLELQGNNIPPSGMVN
                     ATLNAKWLHGAAAANLKAKYELTVSKANASFPEFSSYSFDDPGIDFPTQTQTLWDGQL
                     DDNGTARVYGSLNIPDSRPAAINVLFKGRVFEPGGDFSIDLFSKTFYPFSELVGVKPP
                     QPKMGTSWLETDTDQQFSVVTINNDGKPISCPNLELELYKLTWRWWWEQPETGEAGYV
                     NRNYDYLISSHKFGTVNGKATVKLRVDYPEWGRYYVKVTNKTTGNSCGTIVYFDWPYS
                     SGKSQANRPGGASILAISVDKPDCKVKESFKLTIPGAENSRALVSIENGYKVVKAFWV
                     DASSQANVVDIQTTPEMTPNVFVHVTLIQPHTNKTNDLPIRLYGITRINVSDPETTLQ
                     PVITMPDELKSDKPVEILIREKSGKKMNFTLAMVDEGLLDINRFKTPDPHSVFYAREA
                     IGVKTWDLFDEVIGAYGSTMERLISVGGDEELNPTQGEKIQRFKPVVRFFGPFVLEKG
                     KTEKISFNMGNYFGSVRIMVVAANDGAYGFAEKSCPVKNDLMVMATLPRVLGPQETVT
                     MPISIFTTSPAIKQVKIRVKTSSNLKVLNGNEQNLEFTKPGDLLAFFTLQVSEGTGPA
                     RVEVLAEGNGVKSTQVVDIEVRNPISIQVVSIDTLLPPNGKKSLQLSTFGIKGTNEAY
                     LEASVTPPLNLNKRLGYLIQYPHGCVEQTTSAVFPQLYLSQLVDLTADQQKKTSRFVS
                     EAIKKLAGFQNSDGSFRYWPSYVYGDDWSTSYVGHFLLEARSKGYDVPEGMLSRWTSY
                     QGKSARNYIPRPTNPFNSDLVQAYRLYTLALAAKPEVGAMNKLKEQELGLIAKMRLAG
                     AYALIGQKDVALAIVGSVPIKFSPYRELSYTYGSDIRDRGIVLETLILLGLDSKAYPI
                     ALEISKALSQNDWMSTQETAVALLALSRLSIDKEKGGELNLSYTLAKNSRVISSRRPV
                     AREQLEIRDGSNTLEIQNMTKNNVYASVVLKGVPAPGNETSYSSNLNIMARYRSASGD
                     ELNPYNLKKGMDFTCEVTVSNPGIRGELKELALTQIFPAGWEIQNTRFEGTENVSGST
                     FDYQDIRDDRVITYFSLKPNERRSFTIRLTATYSGRYYMPGPHCEAMYDNAISAGEKG
                     GWVTVE"
     gene            9616..11952
                     /gene="pbpC"
                     /locus_tag="PK502_01660"
     CDS             9616..11952
                     /gene="pbpC"
                     /locus_tag="PK502_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HPG99553.1"
                     /translation="MNFRRNPTGVWQVLTGITCLLVFLLLPPVRFKSETSTVLLDRNG
                     QLLSATVSDEGMWQFPASDTVPNKFATCITQFEDAYFRFHPGVNPFSMVRAAFQNIKA
                     RKVVSGGSTITMQLVRLSRRGKPRTLGEKIVEMAMAIRTEISYSKPRILAMYSSNAPF
                     GGNVVGLDAAAWRYFGVSPQNLSWAEAATLAVLPNAPGLIFPGRNQHLLVEKRNKLLG
                     KLLKRGYIDDETYHLALLEPSPSLTGGLPHEATHLLTRCIHNGFKGKLVRSTIDRELQ
                     ENIMRVAQNHHGYLLANGIRNMAAMVVDNQTGEVMAYMGNVQSNNLSNSPMVDVISSK
                     RSYGSLLKPFLYGLMLSEGLLYPRQLVNDMPVAYAGFAPQNFSHTFEGVVPANEIISR
                     SLNVPSVNLLQQYGIEKFHSSLKQMGITSINKPAGHYGLSIILGGAEASLWELSGIYA
                     ALARQAQGIAAGNISLRYVLDSTTSSAQPFSCSGIGSGAAYLTLEALTQASRPGEDGT
                     WRNFVSSQKIAWKTGTSYGFRDAWAIGVTTCYTVAVWVGNADGEGRANLIGVATAAPV
                     MFDIFSLLPSSVWFSPPWFDLKPVVTCRQSGHQASPACTDTVTEYVPAVRTKTLPCPY
                     HQKVLVDKTHRYRLTQRCASQEEMVEVSWFVLPPAQEWYYSKLHADYRILPPFGKGCT
                     ENPDRLQSMALIYPYSGADIYIPVEAKGVKGQSIWKAAHRNPKATIYWHLNDRFIGKT
                     TGLHEVAVSPSPGVYTLTLVDDNGEMLQRRVRIAGEKK"
     gene            complement(12067..13701)
                     /gene="groL"
                     /locus_tag="PK502_01665"
     CDS             complement(12067..13701)
                     /gene="groL"
                     /locus_tag="PK502_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941147.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPG99554.1"
                     /translation="MSAKVLNFDIEARDKLKRGVDQLANAVKVTLGPKGRNVVMEKKF
                     GSQQVTKDGVSVAKEIELEDPFENVGAQMVKEVASKTSDTAGDGTTTATVLAQAIVNV
                     GLKNVTAGANPMDLKRGIDKAVAKVIENLKKQSVEVGDDYLKIEQVATVSANNDTEIG
                     KLIAEAMKKVKKEGVITVEEAKGIETTVEVVEGMQFDRGYISPYFITNPEKMETALEN
                     PMILITDRKISTMKDLLPVLEPVAQAGRAMLIIAEDIEGEALATLVVNKLRGSLKIAA
                     VKAPGFGDRRKEMLEDIAILTGGVVISEEKGLRLDGAKMDMLGTAEKITIDKDNTTIV
                     NGGGKKDNINKRIAQIRTQIENTTSDYDREKLQERLAKLAGGVAVLYVGAATETEMKE
                     KKDRVDDALSATRAAVEEGIVPGGGVAYIRAIEALENMKGNNEDETTGIQIIKRAIEE
                     PLRQIMTNAGLEGSVIVQKVKEGKGDYGYNAQTNKFENLFQTGVIDPTKVTRVALENA
                     ASIAGLILTTECVITEKKEDKPVPMGNPGMGGMDMM"
     gene            complement(13742..14020)
                     /locus_tag="PK502_01670"
     CDS             complement(13742..14020)
                     /locus_tag="PK502_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPG99555.1"
                     /translation="MSEVKIKPLADRVLVEPMEAEEKTASGIYIPDTAKEKPQKGTVI
                     AVGPGTKDVTMEVKKGDVVLYGKYAGTEISVDGKDYLMMKQSDILAII"
     gene            complement(14314..14661)
                     /gene="secG"
                     /locus_tag="PK502_01675"
     CDS             complement(14314..14661)
                     /gene="secG"
                     /locus_tag="PK502_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPG99556.1"
                     /translation="MYTLFSVLIIIVSILLILIVLIQNPKGGGLASNFSGSNQVMGVR
                     KTADFLEKSTWTLAVALILFTLFATLAMPKKSAEEQKSVIESKVKNTAEPNALPTFPV
                     NPTEGNMGSDTTK"
     gene            complement(14684..15454)
                     /locus_tag="PK502_01680"
     CDS             complement(14684..15454)
                     /locus_tag="PK502_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99557.1"
                     /translation="MDNETLYGIMEARLKPTPETLNQLRETIDEFPYFQGCRVLILKS
                     LSQLGNDEFVAQLRKNAVLLPSRRYLYFTLYPSKPLEETEKIDGGNAEDKKISKPTPK
                     RGRPRKAPTDERKPSFILLDDEKQPAITIHVEASSNTTDTEILELAEGEGEVDSQANG
                     RGLNESEDLIAKFINNQPTISRSTTPGNKDTGENEDISISSVEEPEELASESLAQIYL
                     SQGYLEKAIRVFEKLSLKYPEKSSYFADEIRKIKESTK"
     gene            complement(15459..15974)
                     /locus_tag="PK502_01685"
     CDS             complement(15459..15974)
                     /locus_tag="PK502_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HPG99558.1"
                     /translation="MKRIIRIAIILLPLFVLIQPGCKVSYTFTGASISPETKTFSVQL
                     FQNLAGIVNPTLSSYLTEELKNRFITQTSLSMVDDFGDFAFSGQITQYNVSPISIQDN
                     EVAAQNRLTISVRVKFVNAKDPKLSFDKTFSQYENFDSNQNFVQVEAELVRLIVEKLV
                     DDIFNAAAANW"
     gene            complement(15978..17219)
                     /locus_tag="PK502_01690"
     CDS             complement(15978..17219)
                     /locus_tag="PK502_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPG99559.1"
                     /translation="MELQTIKQRFGIIGNAPGLNRAIDIARQVAPTDLSVLITGESGT
                     GKEVFPQIIHQFSARKHGNYIAVNCGAIPEGTIDSELFGHEKGSFTGATDSRQGYFEV
                     ANGGTIFLDEVAELPHSTQVRLLRVLETGEFIRVGSSKVIKTNVRVVAATNVNLYEAI
                     QEGKFREDLFYRLNTVPIYIPPLRERKEDIYLLFRKFAVDFSEKYRMPPITLDENARK
                     LLTDYSWPGNVRQLKNVTEQISIIEQSRVITVDILQKYIPDNNVPRLPTLYSKKGATE
                     EPSFSSEREILYKILFDMRNDVNELKKLVNVIMQSGVTGAESSFADSAVLHNNFGPVN
                     PIITSPAQPTFEPMSFVKSNNEIQDTEEIMEESLSLEEKEVELIRKALDKHNGRRRQA
                     ASELGISERTLYRKIKEYKLD"
     gene            complement(17433..18890)
                     /locus_tag="PK502_01695"
     CDS             complement(17433..18890)
                     /locus_tag="PK502_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPG99560.1"
                     /translation="MKPKPATTFQRRLNLFDSTAIVVGSMIGSGIFLVSADMARNLGS
                     PGWLLVAWAITGILTVIAALSYGELAVMMPHAGGQYVYLREAYNPLAGFLFGWTLFLV
                     IQTGSIAAVSVAFSKFLGVIFPWFSDNNVLLQVGSLKITTIHLMSIFIILFLTWVNTR
                     GINIGKYVQDTFSYTKIAALLLFFIVGFFIARNDEAVRLNREIFWQGQQLVDGKPVPL
                     SGISLIIALGIAMVGSLFAADAWNNVTFASEEIKDPKRTIPLSMVLGVVIVIVLYMLV
                     NYIYIKVLPVRGSPDATTVMGQGMQYASNDRVGTAAMYGTFGESAAIIMAILIMISTF
                     GCVNGMILSGARVYYAMAKDGLFFKQVGILNSMGVPGVSLALQCAWACILCLSGTYSN
                     LLDYVIFSVLIFYVLSVVGVFILRVKKPDVARPYKTWGYPVTPAVYILACTFIMVILL
                     IYKPAYTWPGLIIVILGIPVYYLWKKPKFSRTKRT"
     gene            complement(19215..20699)
                     /locus_tag="PK502_01700"
     CDS             complement(19215..20699)
                     /locus_tag="PK502_01700"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPG99561.1"
                     /translation="MNRLSTLLFGILSAMPVMSCIAQQPMNRLTYPEVLQLAKEQSPQ
                     AILAKHRFRAAYWEYRTFVAEYRPTLSMNGTIPEFSRSLIRYQNPDGSYSYIEENANT
                     TSLGLSLSQNIGFTGGQIFVKSNLERTDLLGDSSSTSYLSVPVSIGFSQPLFAMNRLK
                     WNKRIEPIKYEEAKKSFIQSMEDISIEASRLFFNLAMAQQNLETARLNYSNTDTLYKI
                     AQGRYNIGTIAENELLQMELSFLNAGTQVNEAEVDLQLKKIQLKSFLGLNDQYDLEII
                     LPTAIPQFELIYDKVMMLARENNPQILSYERQLIEADRDVAQARSERGFNASLYATYG
                     LTQRALNVSDAYKDPQDQQNVRVGITIPILDWGLGKGKVKMAQSNREVIKTTVQQAMT
                     DFEQDIFLKVMQFNRQDDQVMIAEKADYISQNRYEVTKQRFLIGKIDVLDLNVAQSER
                     DQAKQKYISTLGNYWQFYYQMRRLTLFDLENNQPLQADFEMLLK"
     gene            complement(20721..22106)
                     /locus_tag="PK502_01705"
     CDS             complement(20721..22106)
                     /locus_tag="PK502_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG99562.1"
                     /translation="MSMLRVDKLYKRYLHDINIAIEAIMANKLKSALTALGIIFGVAA
                     VISMLAIGNGAQQEILEQMKMVGVNNIIVNSIFEAPDTQTSEEENGTKQKQKFSPGLT
                     LKDVEAIQQIIPSVKRISPEISLNSFVQYDGIRMPAKIMGVSNDYFSLFNLPLEDGVI
                     FDSYHEEHGVPVCVIGSNIKTKFFSTQNPIGQYIKFGSVWLRVIGVLAKSDIKVTGFE
                     SSGINVYNDNIYVPVKSILLRYQNRALVNTRKISESGTSVFVSGGFVAYGGSTQSQAA
                     SNYHQLDRIILQVNQTEDITSTSEVLSRMMLRRHSEVKDFEITVPELLLKQQQRTKDI
                     FNIVLGAIASISLIVGGIGIMNIMFASVMERIKEIGTRLAIGAKKADIIAQFLSEAVL
                     ISVSGGIIGVILGFVLSFLIRHFAGILTIVSPLSILVAFGVSAAVGVIFGLSPAKRAA
                     EKDPIESLRYE"
     gene            complement(22106..23581)
                     /locus_tag="PK502_01710"
     CDS             complement(22106..23581)
                     /locus_tag="PK502_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HPG99563.1"
                     /translation="MRKKVIIGLAVVLAIILAVVLFGSSDKDKAAPQFARVMQGKFEV
                     LVNVTGELKAQNSENIMAPTELRGRTFRISEVKIQDLIAEGTLVDSGDYVATLDRAAL
                     SNQLKSTEDELEKSLQAFLKVQLDTTLQLRELRNNLINLKFDMEEKQLVLEQSKYEPP
                     ATQRQAQINLDKAQRAYEQEVHNYTLKKEQAEASMKEVAINLQRQKRERQDMLDVLDK
                     FEIRAPKPGMLIYYREWNGQKRKVGSSVSPWDLIVATLPDLSVMNSSTYVNEIDISKI
                     KTGQPVRITVDAFPEKKFTGEVIEVANIGEQLPNTDAKVFEVTIRVNEYDPILRPSMT
                     TGNQIITNVYDSVTYIPLEALHANDSISYVYTRKGKMQVVVPGEMNENFIIIEKGLEA
                     NDEVYLSMPEKPEKFSLAGKELISVIKKKKEQKIQDEMRLREVQDSAMKSRSQSQKMM
                     FQMNGQQVRKNGSAPKINSELKGKGNSGSNQANPKPTAPKS"
     gene            24126..26429
                     /locus_tag="PK502_01715"
     CDS             24126..26429
                     /locus_tag="PK502_01715"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPG99564.1"
                     /translation="MVTKISAMVMVATIGMGCINAQQPDYAQWVNPLIGTDNMGHTFP
                     GATVPFGSIQLSPDTDTVPYSLDGKSYNPEVYRYCAGYQYSDKTIVGFSHTHFSGTGH
                     SDLGDILVMPTVGQLQLNPGTSANPEKGYRSRYSHNNEYAKPGYYRVILDDYNIKAEL
                     TATTRVGVHRYTFPQSDSAHIIIDMVHGIYNYPEKNVWTFVRVESDTLVTGYRVTSGW
                     ARTRTVYFAIAFSKPFNTYGLVNDENLAYKGFWRKFNQNENFPENAGRRVKCHFDFTT
                     DSNEAIMLKVAISPVSTANAIENLKMEAPGWSFDNMAQHARDIWNHELGRIDVATLNQ
                     NDKVNFYTSLYHAYLGPTLYMDQDGSYMGLDQNIHKATGFENYTTFSLWDTHRALHPL
                     FNIIQPKRNADMIRSMLAHYSQSVHHMLPVWSHYANENWCMIGYHAVSVIADAAVKGN
                     TAFDLNEALNAAVATAENDWYDGVGYYMQYGYVPEDLSPNSVSKTLEYAYDDWCISQL
                     AAKANRPDLAARFYQRSRNYLNVFDKEIRFMRPRLSNGNFKKEFDVLSTHGQGFIEGN
                     SLNYSLYVPHDPESLASLLGGKEKLASYLDTLFTKYLPDEYFAQTEDITREGLIGTYV
                     HGNEPSHHVAYLYNWSAEPWKTQEKVRMICQTMYKPTPDGLSGNDDCGQMSAWYIFSA
                     LGFYPVAPASEFYSIGSPTVVNATINLENGKTFRISTLNQSPANIYVQNAILNGKPLT
                     GLKLSHSDIMSGGELQLVMGKKPIKRM"
     gene            26683..29181
                     /locus_tag="PK502_01720"
     CDS             26683..29181
                     /locus_tag="PK502_01720"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG99565.1"
                     /translation="MIKPKVLTGFIIILLFSASSIIVTYQGFRELNLTRQEIGQPGQK
                     LAVINRLISGVYGGEADVRMFIITNDTGYLESFEERKKKVTSVLNSLDGLMAETPGQQ
                     QNVKQIKALLSRKWAIVNELLALRKTYTDDRFYEEALKQIERAGTESRIRASVAKTTT
                     VTTTLHDTVVTLENKDENLFGKIKRFFVGPERVDTVSRSVNIETRLDTIDKGQYVSDS
                     IINNLVKLINGIRADQRKYQFDLGVKEAELLEQDRELMKKIESIVSSLERQELSSSIR
                     QSVLAQDLVNRSIIKILALGSFTFILLLVLLAVIFRDISRSNQYRTQLLEAKQYAERL
                     LRVKEQFLANISHEIRTPLSAVIGLTHQLGKTDLNARQQDYVSTLSSSADHLLSVIND
                     ILDYSKLESGQVRFETAMFDPVNVLESVTQTFNPKAEEKQLLVHTNIDPAVPHVLWGD
                     SFRLKQALMNLLSNAIKFTESGSIIVSLGVVRLTPEYVKLQFTVADTGVGIPPEQQQA
                     IFEEFTQADSSISRKYGGTGLGLTLVKKIVELQGGEISLESKINEGTIVKFSIPYALT
                     GVETKEETLERSSFAIPNNTAILVIDDDEVNLLIVTEMATTLGLQVDTLSKPEALYET
                     LSSKKYSAILTDIQMPGLNGYEVVKLVEQFNENIPVVAITANSMVDSPEHFTSHGFAG
                     YLIKPFVETDLQKVLSPLIGIPKPVKQLQVSTKSRRSSPAQIDLSDLYRFTGGDKNSL
                     RLILTSFLDNTYRNADELNRYIREKNLKQASEVAHRMKSAFSQLKIYHISSLLQKIET
                     LKPDKYRAANIYLDYLNKQIKPVVKEIRELLEKL"
     gene            complement(29227..>30785)
                     /locus_tag="PK502_01725"
     CDS             complement(29227..>30785)
                     /locus_tag="PK502_01725"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPG99566.1"
                     /translation="NEIVAACYNDSTGKYWVYTMAMDSSGEWVTNDKPFKRLPNKGAD
                     ALYVDDGGVLWIGIGKELFSFNTRVKRSYNAPYHALVRSVYAKDSLLFNGSFSEVDQD
                     GRKITLLQQPQSKIPQLKYRYNGLVFSFSSPYFEEEAELLYSHYLEGSDETTWSAWDG
                     RTEVTYTNLSEGKYTFHVKAANIFGEESIPATFTFVIHPPWYRTLLAYFCYFIFFIGL
                     VWGLVRWNTRRLIAEKERLEKIVEERTAEVVAQKEEIERQKEKISIQNEEITSSIQYA
                     SRIQSALLTPSELVSRIFPEHFILYLPRDIVSGDFYYVTQVESKKICIVADCTGHGVP
                     GGFMSMLGISFITQILSEVKDLQAADILNRLRQLVISTLHQTSDVGGSKDGMDITIYI
                     VDEQSMTMQFAGANNPLILIRNGEVTQVKGDKMPIGIHLRGEVPFTNNVIEIQKGDVM
                     YTFSDGYVDQFGGELGRKFMIKDFRELLLKIHTYPMAEQRQILLDTLISWQGDTPRID
                     DVVVMGVRIV"
BASE COUNT         8255 a         7366 c         7049 g         8115 t
ORIGIN      
        1 gcggaactag ctcagctggt agggcataat ccgactaccg gcggataagg gtcgccagtt
       61 tgtagtagat ttttgaaaat tttgcggaag tagctcagtt ggtagagcat aaccttgcca
      121 aggttagggt cgcgagttcg agtctcgtct tccgctcagt tctttattaa agttcaaagt
      181 tttgcacttt gaacttttta agtttaatac cgaaaatgcc cgggtggtgg aattggtaga
      241 cacgcaggac ttaaaatcct gtagccattg cggctgtgcg ggttcaattc ccgccccggg
      301 tacgttaaag cctcgcactt gcggggcttt aatatttatc agaatcagct tacacctaca
      361 taaagcagcc actcaccaac ctaagattac tacttttcaa aatataaatt ctacatttgg
      421 ctctatcaac caccacacat ttttaaaaca tgcgagtaca cttcattgcc attgggggaa
      481 gcgccatgca caacctggca attgcgttga aacaaaaggg atacgaggtt accggttcgg
      541 atgatgaact tttcgaacct tcactctcca ggttaaaaca gcatgggctg ctcccgtcag
      601 agattggctg gtatccggaa aggttaacgc ccgatattga tgccgtcgtg ctgggcatgc
      661 acgccagttc agaaaatcct gagttgcaaa aagcccgagt gcttggtttg aaaatatact
      721 cctatccgga gtacctctat gagcaaacca ggggaaaaac cagggtagtt attggtggca
      781 gccatggaaa aaccaccatc acctcgatgg taatgcatgt gttgcagcag gctggctttg
      841 atttcgatta tatggtggga gcccagctgg agggatttga taatatggtt ggacttaaca
      901 accaaaccaa cctggcagta tttgagggcg atgaatattt gacctcacct gtggacccac
      961 gcccgaaatt ccatctttac aagccaacca ttgcagttat ttcgggtatt gcatgggacc
     1021 attttaacgt cttccctacc attgaaaatt accgtgatca atttgtgggt ttcatcagct
     1081 gtattgagcc aaacggaacg ctagtttact gtagcgagga tgatgaggta gcaggggtag
     1141 taagctccac aaaacgttca gacctggagt tgattccgta cacaacacat ccttaccgaa
     1201 tcgagcccaa cgttggctgc cagctggtta ccgagcaggc atgctacccc ttaaaaattt
     1261 ttggtcgcca taacatgcag aacattagcg ctgctaaggc tgtatgctta aaacttggag
     1321 ttagcgaatc gaatttttac cgggcaatct ccaccttcaa gggagctgca aaaagactgc
     1381 aacggctagc cgaaggagca aactccacag tttttctcga ttttgcccac tcgccatcaa
     1441 aagttgccgc cactattggc gctgttctgg aggaataccc caatcgtaag ctggttgcct
     1501 gcctggagct gcacaccttt agcagcttga acataaattt tatgagccag taccgtaatg
     1561 ttttctcagg tgttgcggtg ccgatagttt actttgaccc ggttacagtg gaacacaaaa
     1621 atttacctcc attaaacatt aacgaggtgc gggatgcttt tggacagcct ataccagatg
     1681 tgtgcatttc ggcaagcgaa atggttgaaa aacttaacgg catgaattgg gaaaatgcca
     1741 acctgttgct gatgacttcg gggaactttt cgggtttaaa ccttaacggg ttggctttac
     1801 aaatagcacg atgatttaaa ctttgttaaa gatatcctgt tataggatat tgttcctact
     1861 tttacaacac atattaacta accaaaataa aagcacatga aaatagcaag agtaatagtg
     1921 gtatcgctag cgcttataat ggcggtaaca accgcaagag cgcaggacta caaaaccggt
     1981 ttgggattac gaggcggata cccttcgggt atcacggtta aacatttttt tggcagtaaa
     2041 acagctgttg acggcatcct ttcgtttggc tggggtggaa ttgggattac cggcctttac
     2101 gaattacata accggattcc cagtgctcca ggactcaact ggtactacgg gtttggcgct
     2161 cactttgcca cggctgatgc caacaagaat aatccatttg ccaacgacag gggcgatgat
     2221 gtgtttctag gcgttgatgg ggcaattgga ttggagttta ctttccccga tgctcccatt
     2281 tgcatcagct tggatattct gcctatcctg aacctcatag aatcgcccgg tatttggttt
     2341 aatgcaggcc tttcggttcg ctatgctttt aagtaaaaac agcataaatt ttttcccatt
     2401 tatttgtaga aaataaaaat tcacttacct ttgcacccgc cttggtaggc aatatgctac
     2461 ttagagtaag gcccgttcgt ctaggggtta ggacgtcagg ttttcatcct ggtaacagcg
     2521 gttcgattcc gctacgggct acgaataacg aacctattta taaacggata tggcaaatca
     2581 caaatcagca gaaaaacgaa tcagggaaac cgaaacccga aggcttcaca accgctacta
     2641 tgccaaaact acacgtaacg ccctgaaagc tttacgtaac accactaaca aggcagaagc
     2701 aatagagatg cttcccaagg tttcatcaat gctcgacaaa cttgccaaaa agcatattat
     2761 tcataggaat aaagctgcaa atcttaagag ttccatacaa aagcatgtaa atgccctgca
     2821 gtaagaaagg cttaattttc catgaactgc actttgcgcc tcccacaatc gaggcgtttt
     2881 ttatttacct tcttcggcta ggtacctgaa tcgttttgaa agcatccccc ccatgctgga
     2941 gtatggaatt tcaaagacca aatccccatc ggcaaagctg gctatttcat acacgttgta
     3001 gtgaagcact attccatttt cgctaagtcc aaactccctg ggtaggctga attttcctcc
     3061 ttcaaaccag tagccattca aatgatcaac ccctacccta cccttaaaga gtttttcggc
     3121 caattcggaa aattgcttgg tgttgcttac aatatcatta aggctgagcg gtctggactt
     3181 acgcaggtct atgttttggt aatgatagcc atacattcca tgtgctcccc caaggtaatc
     3241 ttcgtagcgg tactggatgg tcaacacttt tccatcattc aacaggacgt cgtaatcggc
     3301 atttaaatac cacgaaatgc cattgctgcc aggaaataac gataggtaat caacataagc
     3361 actatcatag ctttcggtta accgggtaag aacttcctta aactggttaa ctccctttac
     3421 ctttgtgctg tccgataaaa gtttcaggta gtcgtaggct atcatctttt gaattcgggc
     3481 tgcccttttc ccctgaacaa ccggaaattt aaggagtatt ctaaaacagg aagaatcggg
     3541 ttctacgcat ccacgtgtaa acacaagaga atctggagcc accgataagt tgggtgtata
     3601 ggttgccctt acggtgttaa atagaaaata aaccaacccc ataagaactg ctaacgatag
     3661 aagacgtatt attctggact tcagcatatt actattttcc ctttaaatat tgaaccagct
     3721 ccaggcgagc tattaaacct atttctttac tgctcaaata tagctcgata atttcgaaaa
     3781 atcttatgct aggtctgccc cattcatcac ccatcgtaaa ccttcaaatg ccgctcgcct
     3841 cccaaccgat tgaactacat ataaaatgct taaatacaga tgtactaccc ataaccttgc
     3901 ggtattcggg ttctaaaagc gcattgtttt taaagttcct ttaccttaag aaagtagatg
     3961 gatgtttgtg tgttaccaat atttgtgggc atttcaatat taatgcggta aactcaaaat
     4021 cttgagtaat tttgggtagt agtataaatc atccttcaag catgaacagt aaatcaatca
     4081 aatggctttt aactgttggg gttatagcca taatcatcct tcaggttgga tgtaagggca
     4141 aacaggatgt tgaaatttcg actgatccgc tatttggaga gtacatttca gcatatacct
     4201 cgggaatgat ttcggcacac tccgccatca caattaagct tacaaagcca agcagcaagt
     4261 ttactttcaa tggcgacgag gtacacgaag gctatttcag cttttcacca tcagtaaagg
     4321 gtaaagccta ctgggttgat gaccaaactc tagagtatag accgacagag gtaatgcgtt
     4381 cgggcaaagt ttactcctca tccctggcat taagcaagct ttttaaaggt gtagaaccaa
     4441 agcttaaaac gtttcgcttt agcttcaggg taattccaca atctgttagc attgagctga
     4501 atggtttgat tgttagggat gcagaaaatc caaacagcta tgagctcgat ggcacggttc
     4561 aaacagctga tgttgcagag tccgagaaac tgaacgagtg cataaaggca agcgtcaacg
     4621 gaatgagtat tgctacaaaa cttgattcca ccgaagtcgt caacacctac catttgcgtc
     4681 tggagggaat tgaacgaacc gtaaaaaaag gagaggtaag gatatcatgg aacacctcgg
     4741 caataggagg aaactcaaag gggtatgaaa tccttgaggt gccacccttg ggtagcttta
     4801 aagtgattag cgcaaaagcc attcaaacgc cggagcaatc cgtcaccatt attttttccg
     4861 acctactgga cagaaaccag aactttgagg ggttggttga gcttgaagga atttcggggc
     4921 tgaggtacga agttgaccga aacatgctcc gggtttaccc acccgaaact attgaaggcg
     4981 agaaaaatct taacattagt gctggtgtgc taaatgcaat tggtaaaagg cttaatgctg
     5041 agttttctca aaacctactg tttgaggacg ttaatcctgc acttcgcact gtggggcatg
     5101 gagcaattct gcctacaacc caggggctga ttttctcttt cgaagcagtt aaccttaaag
     5161 cggttagcgt tcagatagtg aagatatacg aaagcaatgt gccctactac cttcaggtaa
     5221 ataccatggg agggactaac gagctaaggc gtgtgggtta ccctgtgctc aacaaggtca
     5281 tcgggttgaa ccagtttggc ccaattgttc cgaataaatg gaccaggtac acgctggatc
     5341 tcgcacaact tttcaaagcc gacaggggcg caatttacca aattagcatt acattcaata
     5401 aaaagcaact tctttcttcc tgcggggatg actccgacca aaccactcct acatctcctt
     5461 ttagccccgg cgatgaagaa gttgcacggg gttacgatgg acccggttac tacgacgact
     5521 actactatta ctacgacgat gattataact ggagagagag agacaacccc tgcaactcgg
     5581 cctactactc agaatcgaga atgattaacc agataataat atcatcagat atcggtatta
     5641 ttgcaaagct aggtaatgac ggtggaatgc tggttgctgt taccgacttg ccttcagcaa
     5701 aaccgttgtc taacgccaac gtaagaattt ccgactacca gaaccaaacc atagccgatg
     5761 gtaacaccaa caacgacgga ttttttgagt ttaaaacaac acgaaggcca tttttggtaa
     5821 ccgcaagtga tggcaagcaa aaaggatact tgcgtgtaga taacgggaca tcgaacccgc
     5881 taagcaactt cgacgttagc gggaatgagg ttcaacatgg cttaaaggga atgatatacg
     5941 gtgaaagggg tgtatggcgc cccggcgata cccttcatct ctgctttatt cttgaggaca
     6001 agggaaaaac acttccgccg aaccacccgg tagtacttga gctgcacgat ccgaagggtt
     6061 tgctggtcag gagagctgtt cagcccaaca acgatgcagg gattttctac tttccaacag
     6121 taaccgatcc tcaagctcct accggaccat ggagcgcaac agttagggtg ggcggagcca
     6181 tttttcaaaa aaccttaaag gttgaaagta taaaaccaaa ccgtcttaaa ataaaccttg
     6241 aacttcaggg caacaacata cccccgtcgg gcatggtcaa cgctaccctt aacgccaaat
     6301 ggctgcatgg cgcagctgcg gcaaatctaa aagctaaata cgagttaact gtaagcaagg
     6361 caaatgcttc gtttcccgaa ttcagcagct actcatttga cgatccggga attgattttc
     6421 caacgcaaac acaaaccttg tgggatggac aactcgacga taatggcacc gccagagtct
     6481 atggatcact aaacataccc gatagccgcc cagccgctat taatgtgctt tttaagggaa
     6541 gagtttttga accgggtggt gatttcagca tcgatctgtt ttctaaaaca ttttacccgt
     6601 ttagcgagct tgtgggagtt aagccaccac agcctaaaat gggaaccagc tggctcgaaa
     6661 ccgacaccga ccagcaattc tcggtagtta ccatcaacaa cgatggcaag ccgataagct
     6721 gtccgaacct ggagctggaa ctctacaagc taacgtggcg atggtggtgg gaacagcccg
     6781 agacaggcga ggccggctac gtgaaccgga actacgacta ccttatatcg agccataaat
     6841 ttggaacagt caacggcaag gctaccgtta agcttagggt tgattatccc gaatgggggc
     6901 gctactacgt taaggttacc aataaaacaa cggggaacag ctgcggaaca atagtatact
     6961 tcgactggcc atacagttca ggaaaatctc aggctaatcg tccgggtggt gccagtattc
     7021 tggctatctc ggttgataag cccgattgta aagttaaaga aagcttcaaa cttaccattc
     7081 ctggcgccga aaattcgaga gctcttgtta gcattgagaa tgggtacaag gtggttaaag
     7141 cattctgggt tgatgcctca agccaggcca acgtggttga catccaaacc acaccggaga
     7201 tgaccccaaa tgtgttcgtc catgttaccc tgatacagcc acataccaat aagaccaacg
     7261 acttgcccat ccgtctctat ggaatcaccc ggattaacgt ttctgacccc gaaacaacgc
     7321 tgcaaccagt aatcactatg cccgatgagt taaaatcgga taaacccgtc gaaattctca
     7381 tcagggagaa gagcgggaaa aaaatgaact ttacgcttgc gatggttgac gaggggttgc
     7441 tcgacatcaa ccgcttcaaa acccccgatc cccactcggt attttacgcc cgggaggcca
     7501 taggggtgaa aacatgggat ttgttcgacg aagtcatagg tgcttacgga agcacaatgg
     7561 aaaggttgat ttctgtaggt ggcgacgagg agctaaatcc tacccaagga gaaaaaatcc
     7621 agcgcttcaa accggttgtc cgattctttg gcccctttgt gctcgaaaag ggtaaaactg
     7681 aaaaaataag cttcaacatg ggaaattatt ttggcagcgt aaggataatg gttgttgcag
     7741 ccaacgatgg agcctatggt tttgctgaaa aaagttgtcc ggtaaaaaat gacctgatgg
     7801 taatggccac ccttccccgg gtactgggac ctcaggaaac agtaaccatg ccaatcagca
     7861 tctttaccac atcgcccgcc attaagcagg tgaagattag ggtaaagacc tcttcaaacc
     7921 tgaaagttct gaatggtaat gagcaaaacc tagaatttac aaaacctggc gacctgttag
     7981 cattcttcac attacaagtt tccgaaggca ccggtccggc aagggttgaa gtgcttgccg
     8041 aaggcaacgg ggtaaaatca acccaggtag ttgacattga agtacgtaat cccatttcaa
     8101 ttcaagttgt aagcattgat accctcctac cgccaaacgg caagaaaagt ttgcagctta
     8161 gcacttttgg cataaaaggc acaaatgaag cttacctcga ggcatcggtt acaccacctc
     8221 taaacctaaa caaaaggttg ggctacctta tccagtatcc ccatggctgc gtggagcaaa
     8281 caacctcagc agtattcccc caactttacc ttagccaact ggttgatctg acagccgacc
     8341 agcagaagaa aacctcacga ttcgtttctg aggctattaa aaagctggca gggtttcaga
     8401 atagcgatgg gagctttcgc tactggccat cctatgttta tggtgatgac tggagtacca
     8461 gctatgtagg ccattttctt cttgaggctc ggagcaaagg gtatgatgtt ccagagggca
     8521 tgctatcgag atggaccagc taccagggaa aatcggcaag aaattacatt ccccggccta
     8581 ccaacccgtt taacagcgat ttggttcagg cataccggtt gtacaccctt gctctcgcag
     8641 caaaacccga agttggcgct atgaataagc ttaaggagca ggaacttggc cttattgcaa
     8701 aaatgaggct ggccggcgcc tatgccctta ttggccaaaa ggatgtagcg ttggccatag
     8761 taggaagtgt tcccattaaa ttcagcccat acagggagct aagctacact tatggctcgg
     8821 acattaggga caggggaata gttctggaaa ccttaatttt gctgggtttg gattcaaaag
     8881 cttatccgat tgccctggag attagcaagg ctctctcgca aaacgactgg atgagcactc
     8941 aggaaactgc agtagcatta cttgccctct ccaggttatc cattgacaag gaaaagggtg
     9001 gcgagctaaa tctatcctat actcttgcca aaaattccag ggtgatttca agtcgcaggc
     9061 ctgttgcccg cgagcagttg gaaatacgcg atggctctaa cactcttgaa attcaaaata
     9121 tgactaaaaa caacgtatat gccagcgttg ttctaaaagg agtacctgca ccaggcaatg
     9181 agacatcgta cagcagcaac ctcaacatca tggctcggta ccgttcagct tcaggtgatg
     9241 agctgaatcc ctataacctc aaaaagggaa tggatttcac ctgcgaggta actgtttcga
     9301 accccggtat ccgtggagaa cttaaggagc ttgcccttac acaaattttc cctgcaggat
     9361 gggaaattca aaacacccgc ttcgagggga cggaaaatgt ttcggggtca acttttgact
     9421 atcaggatat tcgggacgac cgggttatca cctacttcag ccttaagcct aatgaaagaa
     9481 ggagtttcac cataaggcta acagccacct attccggcag gtactacatg ccgggtccac
     9541 actgcgaggc aatgtatgat aacgccattt ctgccggtga aaaaggaggg tgggtaaccg
     9601 tggagtagca agccaatgaa ttttagaagg aatcctacgg gtgtttggca ggtgcttact
     9661 ggtataacgt gcctgctggt tttcttgtta cttccccctg taaggttcaa gagcgaaacc
     9721 tcaaccgtgc tactcgaccg taacgggcag ctgctttcgg ctactgtttc cgatgaagga
     9781 atgtggcagt ttcctgcctc cgatacggtt cccaataagt ttgcaacttg cattacccag
     9841 tttgaggatg cctacttccg gtttcaccct ggcgtaaacc ctttctcaat ggttcgtgca
     9901 gccttccaaa atataaaagc ccgaaaagta gtaagcggtg gcagcaccat caccatgcag
     9961 ttggttcgat tgtcgcgacg aggaaaacca agaaccttag gtgaaaagat tgttgaaatg
    10021 gcgatggcta tccgaacaga aatttcgtat tcaaaacctc gaatactcgc catgtattca
    10081 tccaacgcac cctttggagg aaatgtggtt gggctcgatg cagcagcttg gcggtacttt
    10141 ggagtaagcc cgcaaaatct aagttgggcc gaagctgcta cgctggcagt gctacccaat
    10201 gcccccgggt tgattttccc cgggcgaaac cagcacttgc ttgttgaaaa aaggaataag
    10261 ctgctgggta aactcctgaa aaggggctac attgacgatg agacctacca tcttgccctg
    10321 ctggaacctt ccccaagctt aaccgggggg ctgccccatg aagcaaccca cttgcttacc
    10381 cgttgcatcc acaacgggtt taagggtaaa ctcgtacggt caaccattga tagagaactc
    10441 caggaaaaca tcatgagggt tgcgcaaaac catcatggat acctcctggc aaatggaatt
    10501 cgaaatatgg ctgccatggt agtggacaac caaacgggtg aggttatggc ctacatgggc
    10561 aatgttcaat ccaacaatct atccaactca ccgatggttg atgtgattag ttcaaaacga
    10621 agctacggaa gcctcctcaa gccttttctc tatgggctaa tgcttagtga gggattactc
    10681 tacccccgcc agctggttaa cgatatgccc gtagcctatg caggatttgc gcctcaaaat
    10741 tttagccaca cctttgaagg cgttgtgcca gccaacgaaa tcatttcccg atcactaaac
    10801 gtgccttctg ttaacctttt gcagcagtat ggaattgaaa aatttcacag cagccttaaa
    10861 cagatgggga taaccagcat caacaagcct gcaggtcact atggtctttc catcatactt
    10921 gggggcgctg aggctagcct ttgggagcta tcgggcatat atgctgctct tgcccggcag
    10981 gcacagggga ttgctgctgg aaatattagc ttacgctatg ttctggatag cacaacatcc
    11041 tccgcacagc ccttcagctg cagcggaatt ggttccggtg ctgcctacct tacacttgaa
    11101 gcactaacgc aggctagccg ccccggtgag gatggaacct ggcgaaactt tgtttcgtcg
    11161 caaaagattg catggaaaac cggaaccagc tatgggtttc gtgatgcttg ggctattgga
    11221 gtaaccactt gctacacggt agcagtgtgg gtgggaaatg ccgatggcga ggggagagca
    11281 aatcttattg gcgtagccac agctgcccct gtaatgtttg acattttttc gctgctccct
    11341 tcttcggtgt ggttttctcc gccatggttc gacttaaaac ctgtagtaac ctgcaggcaa
    11401 agcggacacc aggcctcacc agcctgtacc gatacagtta ccgagtatgt tccagctgtt
    11461 aggacaaaaa cgctgccttg cccctaccat caaaaggtgc tggttgataa gactcatagg
    11521 tataggctaa cccaacgctg cgcaagccag gaggagatgg ttgaggttag ctggtttgtt
    11581 ctgccgccag cccaggagtg gtactactcc aaactacatg cagactatag gattttacca
    11641 ccatttggga aggggtgcac ggagaatccc gacaggctgc aatcaatggc gttaatttac
    11701 ccctattccg gcgcagacat ctatatcccg gtagaagcaa aaggcgttaa gggtcaaagc
    11761 atttggaagg ctgcccaccg aaaccccaag gcaactatct actggcatct gaacgataga
    11821 tttatcggaa aaactacagg tttgcacgaa gtagccgttt cgccttcccc gggagtttat
    11881 acgctcacgc ttgttgacga caacggtgaa atgctgcagc gaagggtgag gatagccggc
    11941 gaaaagaagt gagtaaccac aactagcacc aactcgtagg ggttacactt ttccggtagg
    12001 taaggtagat gaaaaaaaac ggaggatatt caatcctccg ttttgtaggc tatgcatggt
    12061 tgaatgttac atcatgtcca ttccgcccat tccggggttg cccattggga caggcttatc
    12121 ttccttcttc tcggtaataa cacactcggt ggtaagaatt agcccggcaa tagaagctgc
    12181 attttccaga gctacacggg taaccttggt tgggtcgata actccggttt ggaataggtt
    12241 ttcaaacttg ttggtttgag cgttgtagcc atagtcgccc ttaccctcct taaccttctg
    12301 aacaattacc gaaccttcaa gaccggcatt ggtcattatc tggcgtaggg gttcttcgat
    12361 agccctttta ataatctgga taccggtggt ttcatcttcg ttatttccct tcatgttttc
    12421 aagagcttcg atagccctga tgtaggcaac acctccaccg ggtactatac cctcttcaac
    12481 agctgcgcga gtagcgctta gggcatcgtc aaccctatcc ttcttctcct tcatttcggt
    12541 ttcggtggca gcgccaacgt agagaacggc taccccgcct gccagcttgg caagacgctc
    12601 ctgaagcttc tcacgatcgt agtcggaggt ggtgttttca atctgggtgc gaatttgggc
    12661 aatacgcttg ttgatgttgt cctttttgcc cccgccattc acaatagttg tgttgtcctt
    12721 atcaattgta attttttcgg cggttcccag catgtccatc ttagcgccat caagccgtag
    12781 gcctttttcc tcgctaatca ccacaccacc ggttaggatt gcaatatcct cgagcatctc
    12841 cttacggcga tcgccaaatc cgggtgcctt tactgcagca attttgagcg aaccgcgaag
    12901 cttatttacg accagtgttg caagtgcttc gccttcaata tcctcagcaa tgatgagcat
    12961 tgcacgtcct gcctgagcaa ccggttcaag aacgggtagc aggtccttca tggtcgaaat
    13021 cttacggtcg gtgataagaa tcatggggtt ctccagagca gtttccatct tctctgggtt
    13081 ggtaatgaag tagggtgaaa tgtatcccct gtcgaactgc ataccctcaa ccacctcaac
    13141 agtagtttcg atacccttag cctcctcaac ggtgattacg ccttcctttt taaccttttt
    13201 catggcttcg gcaatcagct taccaatttc ggtgtcgttg tttgccgaaa cagtggccac
    13261 ctgctcaatt ttaaggtagt cgtcgcctac ctcaacagat tgctttttaa gattctcaat
    13321 caccttggca acagccttgt caataccacg cttcaaatcc ataggattgg ctccagcggt
    13381 aacatttttt aatcctacgt tgacaattgc ctgggccaaa accgttgcag ttgttgttcc
    13441 gtcgccggct gtgtcggagg ttttagatgc cacctcctta accatctggg cacctacatt
    13501 ctcaaatggg tcttccagct caatctcctt ggcaaccgaa acgccatcct tggttacctg
    13561 ctgtgagcca aatttctttt ccattaccac gttgcgtccc ttaggtccta gggttacttt
    13621 tacggcattg gccagctggt caacgcctct tttaagctta tcgcgtgctt cgatatcaaa
    13681 atttaagact tttgctgaca tagttatgct tgatttagtt gtttaacaat gtagaaaccg
    13741 attaaataat agccagaatg tcggattgtt tcatcatcag gtaatcctta ccatcaacgg
    13801 agatttccgt gccggcatac ttgccatata gcactacatc gccttttttt acctccatgg
    13861 taacatcttt tgtaccggga cctacggcta ttactgttcc cttctggggt ttctcctttg
    13921 ccgtatcggg aatgtaaatt ccacttgctg ttttttcttc tgcttccatg ggctcaacga
    13981 gcaccctatc ggctaacggt ttaattttta cttctgacat agcactatat tttaatgtta
    14041 gtattttgat ttttacgaga gggtgtgcat cataatacat gccaaaccgg taaactgact
    14101 ttttttcatg gttcttctgc cacatttgat caatacttcc agcctttggc agaattttcg
    14161 ggcatttagc tccctgcttc gccggaaagc tttgacaatt tataccaaca accaggaatt
    14221 tgccaacatg gcatgaaagc taacgccttg agggataaaa aatgccagca aagaatgctg
    14281 gcattttttt aagtatcatg taagggttac tgcctacttg gtagtatcac ttcccatatt
    14341 tccttcggtt gggttaaccg ggaaggtggg aagggcattg ggctcggcag tatttttcac
    14401 ctttgattcg ataacagatt tctgctcttc tgcgcttttc tttggcatag ccagagtggc
    14461 gaaaagtgta aaaagtatga gggcaaccgc caatgtccaa gttgattttt cgagaaaatc
    14521 ggctgttttc ctcaccccca tcacctgatt tgaccccgaa aagttagaag ccagaccgcc
    14581 tcccttagga ttctgaatca gcactatcag gatgagcagg atggaaacaa taattataag
    14641 cactgagaat agcgtgtaca tcgtaggagt tgttaatgtt aacttatttt gttgattcct
    14701 taatcttccg aatttcgtcg gcaaagtaac tacttttttc cggatatttc aaacttaatt
    14761 tttcgaatac cctaattgcc ttttcaaggt aaccctggct gaggtagatt tgggcaagcg
    14821 attcgctggc cagctcctcc ggttcttcca ccgagctgat agaaatatcc tcattctcac
    14881 cggtgtcctt attacccggg gttgtagaac gagaaatggt gggctggttg tttataaatt
    14941 tagctataag gtcttcgctc tcattcaagc ctcttccatt tgcctgggaa tctacttcac
    15001 cttcgccctc agcaagttcc agaatttcgg tgtcggtggt attactactt gcctcaacat
    15061 gaatggttat tgctggctgt ttttcgtcat cgagtagtat gaatgagggt ttcctttcat
    15121 cggtaggagc cttacgggga cgtcctcttt taggtgtagg tttggagatt tttttatctt
    15181 cagcattacc gccatctatt ttttcagtct cttctagtgg ttttgaagga tatagagtaa
    15241 agtaaagata acgtcgtgag gggagcaaaa cagcattttt ccgaagttga gccacaaact
    15301 catcgtttcc cagttgcgaa agacttttga gaataagtac tcggcaccct tgaaagtagg
    15361 ggaactcatc aatggtttcc ctcagctggt taagggtttc gggtgtaggc ttcagtcgtg
    15421 cctccattat gccgtatagg gtttcattat ccatagcgct accagtttgc tgctgctgcg
    15481 ttgaaaatat catccactag cttctcgact atcaaccgga ccagttcggc ttcgacctgc
    15541 acaaagttct ggttgctgtc gaagttttcg tactgcgaaa aggttttatc gaagctgagc
    15601 ttggggtcct tggcgttaac aaatttgacc ctgacagaaa ttgtgaggcg gttttgcgct
    15661 gctacctcgt tgtcctggat tgaaattggt gaaacgttat actgggtaat ctgcccggag
    15721 aaggcgaaat cgccaaaatc gtcaaccatt gacaggctgg tttgggtgat aaagcggttt
    15781 ttgagctcct cggtgaggta gctgctaagg gtggggttga ctattccggc aaggttttga
    15841 aaaagctgaa cgctgaaggt tttggtctcg ggagaaatgg atgcaccggt aaaggtgtag
    15901 cttaccttgc accccggctg aattaagaca aacaggggaa gaagaataat agctattcgg
    15961 atgattcgct tcatatatta gtcgagtttg tactccttga ttttacggta aagtgtacgc
    16021 tcggagattc caagttcgga agcagcttgt cttcggcgac cgttatgctt atccagagct
    16081 tttcggataa gctccacctc cttttcctcc aacgaaagcg actcctccat tatttcctcg
    16141 gtgtcctgaa tttcgttgtt gctctttaca aacgacatgg gttcaaaggt gggttgtgct
    16201 ggcgaggtaa taatcgggtt caccggtcca aagttgttgt gcagcacggc tgaatcggca
    16261 aaggaagatt cagcaccggt aacgccactc tgcatgatga cgttgacaag ctttttaagc
    16321 tcgttcacgt cgttgcgcat atcaaagaga atcttataga gaatctccct ttcggaggag
    16381 aagcttggtt cttcggtagc accctttttg gagtaaaggg tgggaagtcg gggtacgttg
    16441 ttatcgggga tgtacttctg aaggatatcc accgtaatca cccggctttg ctcaataatg
    16501 gatatttgct cggtaacgtt tttgagctgg cgaacattgc ccggccagga gtaatcggta
    16561 agcagcttgc gggcgttttc gtcaagggtg atggggggca tacggtactt ttcggagaag
    16621 tccacggcaa attttctaaa gagcaggtaa atatcctcct ttcgttcacg aagcggggga
    16681 atgtatatgg ggacggtgtt gagacggtag aagagatcct cccggaactt accctcctga
    16741 atggcttcat agaggttgac gttggtggcg gccaccaccc ttacgtttgt ttttatcacc
    16801 ttagaggagc caacccttat gaactcacca gtttcaagaa cccttaacag ccttacctgg
    16861 gttgagtggg gaagttctgc cacctcgtcg agaaaaatgg tacccccatt agccacctca
    16921 aagtaaccct ggcggctgtc ggtagcccct gtaaacgaac ccttttcgtg cccaaacagc
    16981 tcggagtcaa tagtaccctc gggaatagca ccacagttaa cggctatgta gttgccatgc
    17041 ttgcgtgcgc taaactggtg tattatctga ggaaaaactt ccttccctgt gccgctctca
    17101 ccggtaataa gcaccgacag gtcggtagga gcaacctggc gggctatgtc aatagccctg
    17161 ttcaatccag gggcattccc aattattcca aagcgttgtt ttatggtttg tagctccatg
    17221 acatttccct aacacctgac aaaattacat atttttctca atcttgtatt aatgccttag
    17281 gataaaaaat gccgttgatt ttaactttcg aacagggagg ttacagcatg aacacttttt
    17341 agccacacag ccagctgtct agacgcgctg atttttaaaa atgtagcatt ttcttttacg
    17401 gaaatttcaa catgggcttg tattgcaacc gcttaggtgc gtttagttct agaaaatttg
    17461 ggctttttcc aaaggtagta aacagggatt cccaaaataa ctatgataag gcctggccat
    17521 gtgtaggcag gtttatagat aagcaaaatt accataatga atgtacaggc aagaatatat
    17581 actgccggtg taactgggta gccccacgtt ttataaggac gagctacgtc aggttttttt
    17641 acccttaaga tgaaaacccc caccacggaa agcacgtaga aaatcagcac tgagaatatc
    17701 acataatcca gcaggttgct gtaagtgccc gaaaggcaca gaatacaagc ccaagcacac
    17761 tgaagcgcca gactgacacc aggtacaccc atagaattaa gtatgcctac ctgtttaaag
    17821 aacaggccat ccttggccat ggcgtagtaa acgcgggccc cggatagtat catcccgtta
    17881 acacatccaa aggttgaaat catgatgaga atggccatga tgatggctgc agattcgcca
    17941 aatgttccat acatagctgc tgtgcctacc ctgtcgttcg aggcgtactg cattccctgt
    18001 cccatcaccg ttgtggcatc gggcgatcca cgaacgggta aaaccttaat gtatatgtag
    18061 tttaccagca tgtagagcac tatgacaata acaacgccaa gcaccatact taacgggatg
    18121 gttcgtttgg ggtccttaat ctcctccgag gcaaaggtta cattattcca tgcgtcggcg
    18181 gcaaacaacg atccaaccat ggctatacct aaagcaataa taagcgaaat gcccgaaagg
    18241 ggaacaggtt taccatcaac cagctgctgc ccttgccaga atatctccct gttgagcctt
    18301 acggcttcat catttctggc aataaaaaaa ccaacaatga aaaatagaag caatgctgca
    18361 atttttgtgt agctgaatgt atcctgaaca tattttccaa tgtttattcc cctggtgttt
    18421 acccaggtaa ggaagagtat aatgaaaata gacattaggt ggattgtagt gattttcaag
    18481 ctgccaacct gcagcaaaac attgttatcg gaaaaccacg gaaatatcac cccaagaaac
    18541 ttgctaaacg caactgaaac tgcagcaatg gaaccggttt gtattacgag aaagagcgtc
    18601 cagccaaata ggaaaccagc tagaggattg taggcttctc ttaggtaaac atattgtcct
    18661 ccggcatggg gcatcatcac ggccaactca ccatagctga gtgctgctat aacggtaaga
    18721 attccggtta ttgcccacgc aaccagcaac cacccgggcg agcccagatt tctggccata
    18781 tcggcgctaa ccagaaatat gccagagcca atcatgctac ccaccactat tgctgtggaa
    18841 tcaaataagt ttaatcgccg ttgaaaagtg gttgctggct ttggcttcat tccaatctca
    18901 ataagctact tcttaggggt tgcctgtaaa cagatgcaag aaaattaaaa ttattctgat
    18961 tagcaatacc aaaccccatg aaacggtatg ctccattcaa aaaaaatgct tcaaccaaaa
    19021 cctttattgg tgtctgcata caaccctgta attaggagca acaagcgact attatggaat
    19081 ggatagactg ccaggtagaa gtccgttaag tatgcgaaag cacgcaaggg ctactcttcg
    19141 tgcatattat gattttagct gaattggtgc agcttatcag cgctcgtgct gtaactcact
    19201 gaaaaaggca aatcttactt aagcagcatt tcaaaatcgg cctgaagggg ttggttattc
    19261 tcaaggtcga agagcgttaa cctgcgcatc tggtagtaaa actgccagta gtttcccagg
    19321 gtgctgatgt acttttgctt ggcctgatcg cgctcggact gggctacgtt taaatcgagc
    19381 acatcaatct tgccgatgag gaatcgttgc ttggtaacct cgtacctgtt ttgtgaaatg
    19441 tagtcggctt tttcggcaat cataacctgg tcgtcctgac ggttgaactg catcaccttt
    19501 aaaaagatat cctgctcaaa atcagtcatg gcctgctgta cggtagtttt aattacctcg
    19561 cgattcgatt gggccatttt cacctttccc ttgcccaatc cccaatcgag aataggaatg
    19621 gtaataccca ccctaacatt ctgctgatcc tgcgggtcct tgtaggcatc agaaacattg
    19681 agtgccctct gggtcaaccc ataggtagcg tataggctag cgttaaatcc cctttcggaa
    19741 cgggcttgag ccacgtcacg gtccgcttcg atgagctgcc gttcgtagga aagaatttgc
    19801 ggattgttct ccctggccag catcatcacc ttgtcgtaaa taagttcaaa ttggggaatt
    19861 gctgtgggta gtattatttc cagatcgtac tggtcgttta gccccaggaa cgacttaagc
    19921 tgaattttct taagctgaag gtccacctca gcctcgttaa cctgagttcc ggcattaagg
    19981 aagctgagct ccatctgtag cagctcgttt tcggcaattg taccgatgtt gtagcgccct
    20041 tgggcaattt tataaagggt gtcggtgttg gagtagttaa gcctggccgt ttcgaggttt
    20101 tgctgggcca tggcaaggtt aaaaaacaac ctgctggcct caatggaaat atcctccatg
    20161 gactggataa agcttttctt ggcctcctcg tacttaatag gctcaattcg cttattccac
    20221 tttaggcggt tcatggcaaa caggggctgg gagaaaccaa tggaaacagg cacgctaagg
    20281 taggaagtgg agctgctgtc gcccagcagg tctgttcgtt ccaggttgct tttaacgaaa
    20341 atctggcctc cggtaaaccc aatattttgc gataacgata ggccgagtga ggtggtgttg
    20401 gcattctcct caatgtagct atacgacccg tcaggatttt ggtagcgaat tagacttctg
    20461 ctgaattcgg gaattgttcc gttcatgctc agcgttggcc tgtattctgc cacaaatgta
    20521 cggtactccc agtatgccgc tctaaaccta tgcttggcaa ggatggcctg gggcgattgt
    20581 tctttggcca gctggagcac ctccgggtag gttagccggt tcatgggctg ctgggcaata
    20641 cagctcatta ccggcattgc tgatagtatc ccgaatagta gggttgacaa cctgttcatc
    20701 tcaacaaatt attagcgttt ttactcgtag cgtaacgact caatagggtc tttttcggca
    20761 gcacgcttgg caggcgacaa gccaaaaatg acgcccacgg cagccgatac cccaaatgca
    20821 accaaaattg aaagtgggct gactatggta agtattccgg caaagtgcct gattagaaac
    20881 gatagcacaa agcccagtat cactccaata attccgcccg aaacgctgat gagcactgct
    20941 tcggaaagga attgcgcaat tatgtcagct ttcttggccc caatggccag gcgtgttcct
    21001 atctccttta tacgctccat aaccgaggca aacatgatgt tcataattcc aatcccgcca
    21061 acaatgagcg agatgctggc aatggccccc agcacaatgt tgaatatgtc cttggtgcgc
    21121 tgttgctgct tgagcagcag ctctggaacg gttatctcaa aatccttcac ctcggaatgg
    21181 cgcctgagca tcatccggct gagcacctcg gaggtggagg taatatcctc ggtttggttc
    21241 acctgcagga taatccggtc gagctggtgg tagttggagg ccgcttgtga ttgcgtgcta
    21301 cctccatagg cgacaaaccc accgctgacg aatacgctag ttcccgattc actaatcttg
    21361 cgggtgttta ccagcgccct gttctggtag cgaagcagta tgcttttcac cggcacgtag
    21421 atattatcgt tgtacacgtt aatcccgctg ctttcaaatc cggtaacctt aatgtccgat
    21481 ttagccagca cgcctatcac ccttagccat acactgccaa acttgatgta ctgcccaatg
    21541 ggattctggg tgctaaaaaa cttggtcttt atatttgaac ctataacaca aaccggaaca
    21601 ccatgctcct cgtggtagct gtcgaagatt accccatcct ccaggggaag gttaaagagg
    21661 gaaaagtagt cgttggatac tcccataatt ttagcgggca tacgaattcc gtcgtactgg
    21721 acaaacgagt taaggctgat ttcggggcta atccttttaa ccgaggggat tatctgctga
    21781 atggcctcca catcctttag tgtcagcccg ggcgaaaatt tctgtttttg cttggtgcca
    21841 ttctcctcct ccgaggtttg ggtgtcgggt gcctcgaata tggagttaac gatgatgttg
    21901 ttaacgccaa ccatcttcat ctgctccagt atctcctgct gggcaccatt ccctatggcc
    21961 aacatgctga taactgcagc cacaccaaag attatgccca gcgctgttag ggccgatttt
    22021 agcttattgg ccataattgc ctcaatggca atgttgatgt cgtgcaggta gcgcttgtag
    22081 agcttgtcaa ctcgcagcat actcatcagg atttaggagc ggttggttta gggtttgctt
    22141 ggttgctacc agagtttccc ttccctttaa gctcactgtt gattttcgga gctgagccat
    22201 ttttcctcac ctgttgccca ttcatctgaa acatcatttt ctgggactga gacctgcttt
    22261 tcattgcaga atcctgcacc tcgcgaagcc tcatttcatc ctgaattttt tgctcctttt
    22321 tcttctttat aaccgaaatg agctccttcc ccgcaagcga aaacttttcg ggtttctcgg
    22381 gcatcgaaag gtaaacctca tcgtttgcct ccagcccttt ttcaataatg atgaaattct
    22441 cattcatttc gccaggaacc accacctgca ttttcccctt acgggtatag acgtagctga
    22501 tggagtcgtt cgcatggagc gcctcgagag gaatgtaggt taccgagtcg taaacattgg
    22561 taattatctg gttaccggtt gtcatggagg gtcgaagtat cgggtcatac tcatttaccc
    22621 tgatggttac ttcaaaaacc ttggcatcag tgtttgggag ttgctcgcca atattggcta
    22681 cttcaataac ctcaccggtg aatttttttt caggaaaggc atcaacggta atacgaacgg
    22741 gttgtccggt tttaatttta ctgatgtcta tttcgttaac gtaggtgctg gagttcatta
    22801 ccgaaaggtc gggaagggtg gcaacaatca aatcccaggg tgaaacgctt gaccccacct
    22861 tgcgtttttg cccattccac tcgcggtagt agataagcat acccggcttg ggtgcacgta
    22921 tttcaaactt atcaagcaca tcaagcatat cctgcctttc tcgtttttgg cgttgaaggt
    22981 tgatggccac ctccttcatt gaggcttcgg cctgctcctt tttcagggta tagttgtgaa
    23041 cctcctgctc gtatgcacgc tgagccttat caaggtttat ctgagcctgc cgttgggtag
    23101 ccggtggttc atacttcgac tgctccagca ccagctgttt ctcctccatg tcaaacttca
    23161 ggttgataag gttgttcctt agctcgcgca gctgcagggt ggtgtcgagt tgaactttca
    23221 ggaaagcctg aaggcttttc tccagctcgt cctcggtgct ttttagctgg ttgctaagcg
    23281 cagccctatc gagcgttgcc acgtaatcgc cagagtccac aagggttcct tcggctatca
    23341 aatcctgaat tttaacctcc gagattcgga atgtccggcc tcgcagctcg gttggggcca
    23401 tgatgttctc tgaattttga gctttcagct cgccggttac gttaaccaaa acctcaaatt
    23461 ttccctgcat aacccgggca aactgtggtg ctgccttgtc cttatcggac gatccaaaga
    23521 gaactaccgc aagtataatt gccagaacaa cggccaggcc aatgattacc ttttttctca
    23581 tattcacctt ctgtttttgc taagaatttg agttgaaaat agcaaatagg tttaagctgg
    23641 taaaacattc tggcctaaaa atataaaata tgcgctaccc tactggcctc ttcaactttt
    23701 aatattgtca cttctcctaa tgtcctgaaa attttaggtt tattgcctaa caaaagggat
    23761 ggggaggctt ttcaaaatac tgctctaatt tttactgcaa aactatacta caaagttgtc
    23821 gaggagtaaa actcggaacc cgccatcgca ctgcttggcc atcaggtttg atgggaaaat
    23881 cacccttttt ttttacactt tcaacatcaa cagccgaaca aaataaaatt accaattatc
    23941 ccatatctga tggtttttaa aatcaacatt tccggccgat tatcatcaat tcaaccaaaa
    24001 gccactcaac agttttaggt tctatcggct aaagcagcct ttgcgagctg ctgcgagctg
    24061 ctgattattc ggtaaacatg tatcttcaaa aaaattacct ttacaacaat ttataaacca
    24121 cgctaatggt tacaaaaatt tccgcaatgg ttatggtagc aacaattgga atgggctgta
    24181 taaacgccca acagccagac tatgcacaat gggttaaccc cttaattgga accgacaata
    24241 tggggcacac ctttcccggg gccaccgttc cattcggttc catccagcta agccccgaca
    24301 ccgataccgt tccctactcg cttgatggta aaagctacaa tccagaggtt taccgctact
    24361 gcgccggcta ccagtattcc gacaagacaa ttgtgggttt cagccacacc cattttagcg
    24421 gaacgggaca ctccgactta ggcgatattc tggtaatgcc tacggtgggc cagctgcagc
    24481 ttaaccccgg tacatccgct aatcccgaaa agggctaccg ttcgagatat agccacaaca
    24541 acgagtatgc caagccggga tactaccggg tgattctcga cgactacaac atcaaggctg
    24601 aacttactgc taccacacgc gttggtgttc accgctacac ctttccccag tctgattcgg
    24661 cccacattat catcgacatg gtccacggca tctacaacta tccagaaaaa aatgtttgga
    24721 ctttcgtcag ggtcgaaagc gatacgctgg ttacaggcta cagggtaact tcgggctggg
    24781 ccagaacgcg caccgtttac tttgccatcg ccttctccaa accctttaac acctatggcc
    24841 tggtgaacga cgaaaacttg gcttacaagg gtttctggcg taagtttaat caaaacgaga
    24901 attttcccga aaatgcaggc cgacgggtga agtgccattt cgatttcact accgacagca
    24961 acgaggccat tatgcttaaa gtggccatat cgccggtaag caccgcaaat gccattgaga
    25021 accttaaaat ggaagccccc ggttggagct tcgataacat ggctcaacat gccagagaca
    25081 tttggaacca tgagctggga agaattgatg ttgccacact caaccaaaac gacaaggtaa
    25141 acttctacac ctcgctttac catgcctacc tgggacctac cctttacatg gatcaggatg
    25201 gaagctacat ggggctcgac caaaacattc ataaagcaac cgggtttgaa aactatacca
    25261 ccttttcgct ttgggatacc catagggcgc tacaccccct tttcaacatc atacaaccca
    25321 agcgaaacgc agacatgatt aggtcaatgc tggcacacta cagccaaagc gttcatcaca
    25381 tgctacccgt atggtcgcac tacgccaacg aaaactggtg tatgattgga taccatgcag
    25441 tttcggttat tgctgacgct gcagttaaag gaaatacggc atttgacttg aacgaagcgc
    25501 ttaatgcagc cgttgcaact gccgaaaatg actggtatga tggcgttggc tactacatgc
    25561 agtacggtta cgttcccgag gacctatcgc ccaactcggt ctccaaaacc ctggagtacg
    25621 cctacgacga ctggtgcatc tcccagcttg cagcaaaagc aaatcgaccc gaccttgcag
    25681 ccaggttcta ccagcgatcc cgaaactacc tcaatgtttt tgacaaggaa atacgcttta
    25741 tgaggcctcg cctctccaac ggaaatttca aaaaagagtt tgatgttctg agcacccatg
    25801 gacaggggtt tatcgagggt aactcgctta actactcgct ctatgtgccc catgaccccg
    25861 aaagcctggc ttcactcctg ggtggaaagg aaaagctggc cagctacctt gatacccttt
    25921 tcaccaagta cctacccgat gagtactttg ctcaaaccga ggatattaca cgcgaagggc
    25981 tgattggaac ctatgttcac ggtaacgaac cctcgcacca tgtggcctac ctctacaact
    26041 ggtctgcgga accttggaaa acacaggaaa aagtccgaat gatttgtcaa accatgtata
    26101 aacccacacc cgatgggtta agcggtaatg atgattgtgg ccagatgagc gcatggtaca
    26161 tttttagcgc tctcggattt tatccggttg ctccggcatc ggagttttac tccattggta
    26221 gccctaccgt ggtaaacgct acaattaacc tggaaaatgg caaaactttc cgcatttcaa
    26281 ccttaaatca atcacctgca aatatttacg tgcagaatgc aatccttaat ggaaaaccat
    26341 taaccgggtt aaaattgtcc cattctgaca taatgagcgg cggtgagctg cagctggtaa
    26401 tggggaaaaa accaataaaa aggatgtaaa atataggggc tgccaggcta aatttcattc
    26461 tctaaaacct ttatacatcc tgttacgtgt gggtattcca ccaggtgtaa agaaattttc
    26521 acctcttaaa ccaggcacag gcacgagtgc tcttgatagc tttgtagaaa atggagggct
    26581 accccacccg gaccaattca gccaggtgca acgtaaattt ttctaccttt gaagtgttgt
    26641 taaactgtta tggccgattc gcttgacaaa ccaaagggct tcatgataaa accaaaagtg
    26701 ctaacgggtt ttatcatcat tctgctattc tcggcctcat ccattatagt aacttaccag
    26761 ggtttcaggg agctgaacct tacccggcag gagattggac aacccggcca aaaactagcc
    26821 gtcatcaacc ggctgataag cggggtgtac ggaggtgaag ccgacgtaag aatgttcatt
    26881 attaccaacg acacgggcta cctcgaaagt tttgaggaaa gaaagaaaaa ggttacatcg
    26941 gtgctcaact cgctggatgg cttaatggct gaaacgccag ggcagcaaca gaatgttaag
    27001 caaataaaag ccttactttc acggaaatgg gcaattgtaa atgagctcct tgcactcaga
    27061 aaaacctata ctgacgatag gttttacgag gaggcgctaa aacagattga aagagccgga
    27121 accgaaagcc gtataagggc atctgtagcc aaaacaacca ccgtaaccac caccctgcac
    27181 gacacggtgg taaccctcga gaacaaggat gaaaaccttt ttggaaaaat aaagcgattt
    27241 ttcgttggtc ccgagagggt tgacacggtt tcgcgaagcg ttaatatcga aacccggctc
    27301 gatactattg ataaggggca gtacgtttcc gattcgataa tcaacaacct ggttaagctc
    27361 attaatggca tcagagccga ccagcgtaag taccaattcg acctgggcgt aaaggaagcc
    27421 gagctgctgg agcaggacag ggagctgatg aaaaagatag agagcattgt ttcatcactc
    27481 gaaaggcagg agctttcttc gtccatacgc caatcggttc ttgcgcagga cctggtaaac
    27541 cgctccatca taaaaattct agcgctaggc tccttcacct ttatcctgct gcttgtcctg
    27601 ctggctgtta tattcaggga tatttcgcga agcaaccagt accgtaccca gctgctggaa
    27661 gctaagcagt atgccgaacg tctcctgaga gtaaaggagc aatttttggc taacatcagc
    27721 cacgaaatac gaacgcctct tagtgcggtt attggtctaa cgcaccagct cgggaaaacc
    27781 gacttgaatg ccaggcagca ggactacgtc agcacgctca gtagctcagc cgatcacctg
    27841 ctgtcggtaa tcaacgacat tttggactac tccaagctcg agtcagggca ggtgcgattt
    27901 gaaacagcaa tgttcgaccc ggtgaatgta ctggaaagcg ttactcagac ctttaatccg
    27961 aaagctgaag aaaagcagct gctcgttcac accaacattg accctgccgt tccgcatgtt
    28021 ttatggggtg attcgtttcg cctgaaacag gcgcttatga acctgcttag caatgccatt
    28081 aagttcaccg aaagtggaag tattatagtc agccttggcg tggtaaggct aactcccgag
    28141 tatgttaagc tgcagttcac agtggccgac acgggagttg gaattccccc tgaacagcag
    28201 caggctattt ttgaggagtt cacccaggcc gacagcagca tttccaggaa atacggagga
    28261 acaggacttg ggctcacgct ggtgaaaaag attgttgagc ttcagggggg tgaaatttcg
    28321 ttggaaagca aaatcaacga aggaacaatt gttaagttct ccattcccta cgccttaaca
    28381 ggggttgaaa cgaaggagga gactttagaa agaagcagct ttgccatacc aaacaatacg
    28441 gctattctgg taattgacga tgacgaggtt aacctgctaa ttgtaaccga aatggctact
    28501 actctcggat tgcaggttga tacgctgtct aagcccgaag cactctacga aactcttagc
    28561 agcaagaagt atagcgccat tcttaccgac atccagatgc cggggcttaa cggctacgag
    28621 gtggtgaagc tggttgaaca gttcaacgaa aatattcctg ttgtggccat caccgccaat
    28681 agcatggttg acagcccgga gcacttcacc agtcatgggt ttgccgggta cctgataaaa
    28741 ccctttgtgg aaaccgacct gcaaaaggtt ctgtcgcccc tgattggcat tccaaaaccg
    28801 gtgaagcagc tgcaggttag cacaaaaagc aggcgtagct ctccggcaca gattgatctg
    28861 tcagatttat accgatttac cggcggtgat aagaactccc tcaggttgat actaacctcc
    28921 ttcctggaca acacctaccg taatgccgac gagctgaacc ggtatattcg tgaaaaaaat
    28981 ttaaagcagg cctcggaagt tgcccatcga atgaagtcgg catttagcca gctcaaaatc
    29041 tatcacattt cgtccctgct ccaaaaaata gaaacgctca agcccgacaa gtacagggct
    29101 gccaacatct acctcgacta ccttaacaag caaattaaac cggtggtaaa agaaattcgt
    29161 gagctgctgg agaagttgta gccggggtaa acccgaagtt ttcccaaaca tcggatttta
    29221 atcaggtcaa acaatcctta cacccattac cacaacatcg tcaatccgtg gagtatcgcc
    29281 ctgccacgaa ataagcgtgt cgagcagaat ttgcctttgc tcggccattg ggtaggtgtg
    29341 aattttcaga agcagctccc tgaagtcctt aatcatgaat tttcgcccca actcaccgcc
    29401 aaactggtca acatagccat cggagaaggt gtacatcaca tcgcccttct gaatttcgat
    29461 gacgttgttg gtaaacggca cttcaccacg aagatgaatt ccaatgggca ttttgtcgcc
    29521 ctttacctgg gtaacctctc catttcttat caggataagc gggttatttg cccccgcaaa
    29581 ctgcatggtc atggattgct catcaacaat gtagatggtg atgtccatgc cgtccttgct
    29641 accgccaaca tcgctggtct ggtgcagcgt gcttataacc agctgccgca gcctattcag
    29701 gatgtcggct gcctgtagat ccttaacctc gcttaatatt tgggtaataa aggagatacc
    29761 cagcatactc ataaatccac ccggtacgcc atgacccgtg cagtcggcaa ctatacatat
    29821 ttttttgctt tccacctggg ttacgtagta aaaatcgcca ctgacaatat ccctgggtaa
    29881 gtatagaatg aagtgctcgg gaaatatcct gctgaccagc tcggatggtg taagcagtgc
    29941 gctttgtatc cggctggcat actgaatact gctggttatc tcttcattct gaattgaaat
    30001 tttctccttt tgcctttcaa tttcctcctt ttgggccaca acctcagcag tgcgctcctc
    30061 aacaatcttt tcaagtcgtt ccttttcagc aatcagcctt cgggtgttcc accttaccaa
    30121 cccccaaacc agccctataa aaaagataaa gtagcaaaaa tatgccagaa gggttctgta
    30181 ccatggtgga tggatgacga aggtgaaggt agccggaata ctttcctcac caaaaatatt
    30241 ggcagctttt acatgaaagg tatatttccc ttcactcagg ttggtatagg taacctcggt
    30301 tctcccatcc catgcgctcc aggtagtttc atccgaaccc tccaggtagt ggctgtagag
    30361 cagctcggcc tcctcctcaa aatagggcga agaaaagcta aaaactaagc cattgtaccg
    30421 gtactttagc tggggaatct tgctttgagg ttgctgcaga agggtaatct ttctaccatc
    30481 ctggtccacc tcggaaaaag agccgttgaa aaggagggaa tccttggcat aaacgcttcg
    30541 aaccaaagca tggtatggag cgttgtagct ccgcttaacc ctggtattga aactgaacag
    30601 ctccttccct atcccaatcc atagcacccc gccatcatca acatacagag catcggctcc
    30661 tttgttggga agccttttaa aaggcttgtc attggtaacc cattcgccgg atgaatccat
    30721 cgccatcgtg tacacccagt acttgccagt agaatcgttg taacaggcgg caacaatttc
    30781 attcc
//
LOCUS       DAOPHX010000011        29692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_1521_length_29692_cov_6.949118, whole genome shotgun sequence.
ACCESSION   DAOPHX010000011 DAOPHX010000000
VERSION     DAOPHX010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 29692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29692
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1521_length_29692_cov_6.949118"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /gene="pdxH"
                     /locus_tag="PK502_01730"
     CDS             <1..840
                     /gene="pdxH"
                     /locus_tag="PK502_01730"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HPG99567.1"
                     /translation="FRVEFLFSKGIGMIFFPMLQYKKQGVEAEIITPVRVEQLPATFV
                     SGLMLLCYFRATESKDVIIFSGTMGDYVQLRREYMLKSLRKMDVLPNPFEQFDIWFKE
                     AVNAKLPDPNAITLATSTFEGRPSARVVLMKHYDSDGIAFFTNYESRKARNLLKNPYA
                     AMVFFWPELERQVRIEGKVSRVSEQQSDRYFQTRPEGSKIGAWASPQSSVIPNRRYLE
                     QLHADFMEEFQGTTIKRPPNWGGYILQPALFEFWQGRENRLHDRIQYSLVNEKWLIER
                     LAP"
     gene            1330..2490
                     /locus_tag="PK502_01735"
     CDS             1330..2490
                     /locus_tag="PK502_01735"
                     /inference="COORDINATES: protein motif:HMM:NF040309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6051 family protein"
                     /protein_id="HPG99568.1"
                     /translation="MHDELEIVEYNFESSSHKILPGVNKYHCTLHNLDIECNYTYSTE
                     VGNPNSNVDTKDICIEENRFYKYQIIKPKGEATTQRVVFLFHGFNEKDWGKYVPWAKA
                     ISESTGFSVILFPIAFHMQRAPKGWSNKREMFKLSEMRKRSYPNVVHSTLSNVAISIR
                     LHSMPQRFIWSGLQTYYDVIQLIEECKEGKHPYISKNFEFDFFAYSIGGFLAQILKLS
                     NYNNYFANSKVCLFCSGATFNRLSPVSKFILDSEANIALYSFLIEHFDSVLQKDNLLH
                     HYITEHLEGQVFRSMLNYQRMRDLREGLLKKYEDQIYAITLKKDLVIPSFEVINTLKG
                     AYRTINIKVEELDFNREYIHENPFNADSSASQLVDEDFKLVFSKVSDFFKKG"
     gene            complement(2793..6608)
                     /locus_tag="PK502_01740"
     CDS             complement(2793..6608)
                     /locus_tag="PK502_01740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPG99569.1"
                     /translation="MASRIVLLVTNKVLPSFQFEPRLDGMGYSLITLDRIGSDVELPA
                     FDLAIVGVADRASYNAILKVFRKYPEKPLLFYSGKNTADAQKSIPELDSTNHVNPDFT
                     NHELSLQVELAITRKWFDHSSIFSDGNAGSNHVRLKKILSEEQAWRVLFEQSPNGVLL
                     SDSAGSIIFTNNATSEILGYSSSQLLKMRFHDLVPPETLPQVDQNIKKILAGEQLITE
                     VYNLRKDGSKRFVQLHESRVLFPSGEFGIMVVSIDMTRAKKAEDALGESLEKYRILVE
                     QSNDGIIYSKDGIITYGNPRIAELLEVQHCELVGKPFYTYLPKSEWEKLQNRYKNRLE
                     GIKEPSIYETLLVSAHGSNIPVELNVNLATIGNERVTMVVVRDISLRKEAEKAIRESE
                     ESYRGLFNNSGEAIYIIDASGILLDVNPSGLKLFGKEKEETLGLTLMELADQEKSNTE
                     EVKRKIRIAHQGQTQYLEFWGKKSNGDRVPMDIVLSKGTYFGLQVVLAIGHDITERKN
                     AEAILRESEDKFRTLTEQIPVGIYRLTPEGKILYGNPAFAKTLGFNDTSEVIGHSMDE
                     FIATNYDDWVEESVKLSPGAPAEQSFRRQDGINLWANNQLRRVKNASGEVDYLDGVLV
                     DITEHKFTLDALKESEERFRAMVTAIPDRLFRINADGDLIDFAPSEQLLDFELNAGMI
                     GHSLSEFLPQDVFQKFTDAISLAKQSGKLQTFEYKFSKNNQDYFNEVRLLQSGRSLFL
                     VLQRDITQKKQFEAQANMLAQTIMTANDSISITNTDGHFIFVNPAFTKTYGYTQSEII
                     GKHTSILKTPDRDLTLDKTIHEETLKGGWQGELINVRKDGAVFPIFLSTSAVYDENGI
                     AIAHVGIANDITERKKIEQELIRAKEKAEESDRLKTAFLSNMSHEIRSPMNAVLGFTQ
                     LIKSDEQLSETGMQYIELIQNSGKQLLSLLEDIIDISKIQSNQLRIDKNAFDLNELMD
                     ELYLIFSNQLKLKDECKTLLFKPEKTCVSPFVIYSDPVRIKQILSNLLSNAIKFTPSG
                     SINFGYSIFSDDDASKIQFYVRDTGIGISIENQLLIFERFRQADNSYTRLYGGSGLGL
                     AISRGLVELLGGDIWVESKPGEGSEFFFNIPLEFSEKDTPAPVQPSSEVKQPVSQLKG
                     KTVLVVEDVNEIRLYFERVLERTGAKILFAKNAKEARKLFKSNNRIRLVLLDIGLSDA
                     DGFELAVEFKRMRPNTPIIAQTAYALQSELNRSIESGCDDYITKPLDSAVLIGKMLRL
                     IGSKSQQSD"
     gene            complement(6868..7932)
                     /gene="arsB"
                     /locus_tag="PK502_01745"
     CDS             complement(6868..7932)
                     /gene="arsB"
                     /locus_tag="PK502_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HPG99570.1"
                     /translation="MPSKSRLKFFDRYLTLWIFLAMLVGVLSGYLFPSIAVFWDSLKA
                     SPESTTNIPIAIGLILMMYPPLAKVKYEELGDVFRNTRVLSLSLIQNWVIGPILMFLL
                     AIVFLRFNIDYMYGLILIGLARCIAMVIVWNELAGGDTEYAAGLVAFNSVFQVLFFSV
                     YAYLFIAILPGWFGLPVNETISQITIGQIATSVFIYLGIPFIAGFLTRLVLTRIKSKA
                     WYNTHFIPKISPLTPIALLFTILVMFSLKGEYIVKIPLDVVRIAIPLMIYFVVMFLIT
                     FFMSRRIGASYRQTATLSFTAASNNFELAIAVAIAVFGINSGEAFAAVIGPLVEVPVL
                     ISLVNVSLKLKKKYFSQQAV"
     gene            complement(7937..8356)
                     /locus_tag="PK502_01750"
     CDS             complement(7937..8356)
                     /locus_tag="PK502_01750"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HPG99571.1"
                     /translation="MKILILCTGNSCRSQMAEGFLKLFGGNFEVHSAGTQPAPAVNSY
                     AIKVMGEVGIDISHNRTHHVDEFLNEEWDYVITVCGGARESCPAFIGKVKNRLHIGFE
                     DPAEAQGSDEEVLTVFRRVRDQIGEEFRKFHDNLLKP"
     gene            complement(8450..9289)
                     /locus_tag="PK502_01755"
     CDS             complement(8450..9289)
                     /locus_tag="PK502_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99572.1"
                     /translation="MRKLALLILFCILSELGLYLQAQPLVEIYLNSRGSESRGHAFKS
                     VEELHEVLVLGQTKTPSFRVVKQFNAQGKVVSEIKYNSAGGVQNEVRWEYNINGYPIR
                     KTTHQFINYKGWVNEEITLKYNDTTGYLNEINKSVESKIINTALVSCDSTGRIVEVRV
                     FNEKGELSGIDRLIYVPANNLIRVLSYKANDQFVMATAYPLDPNKPEPKSTLKREFNS
                     HGDVILELLPGSKIGQGYYYEYSYDTFGNWTEKLTYQCTVTPNGNVKDKKLEHRITRK
                     IVY"
     gene            9672..10352
                     /locus_tag="PK502_01760"
     CDS             9672..10352
                     /locus_tag="PK502_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPG99573.1"
                     /translation="MKKRLVVLTGAGVSAESGIKTFRDSDGLWEEYDVMEVASIEGWS
                     RNPQLVLNFYNERRRQLQKAMPNPAHLSLAKAESDFLVYIITQNIDDLHERAGSTKVL
                     HLHGELKKARSTVNPDLVYDIGYSDIHWGDMCELGSQLRPHVVWFGEAVPAMEEAVKL
                     TQTAELFAVVGSSLNVYPAAGLLSYVPRQCPIFLVDPKEVATFRDDITVIREPASTGV
                     PKMLRLMA"
     gene            10760..12499
                     /locus_tag="PK502_01765"
     CDS             10760..12499
                     /locus_tag="PK502_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG99574.1"
                     /translation="MKAGYRGFLAAAVAVLLVSLVYPSGVFSQDLSQQIANYEQLAAE
                     FEKNGDANQAAYYFDKAANQAWQARDLNRAIANFDRALANIKQVGNVNGAKTIYTNLG
                     LIYCEKGNHNEAYNNFLAALAAARSLGRKDDIANALVNISTEQIEMSDFTGAQKSLDE
                     AQRLAQEVDNARALKNIYFNYNKLYEKLGSKDKADEYFRLYMMLNKKLQDDEFKARQM
                     LAQAKVDSAGRIINQISYEKESTARKLMETSHELQQTTNILKETERISTEQRMQIDLL
                     NTQMKLREAEIRNQHLLQRIYIGLIIILLMFAAILFWANNQKQKANKLLSEKNAEIFA
                     QKEEISRQADELRELNALKDRLFSIIAHDLRSPLFSLITMLNIAKEGHFTEESFKEII
                     SELSSNVSHTTALLENLLTWAKNQMQGTKVKPERFFLNEVVTPRLQQLNEVALQKNIV
                     ITNHVDESTSVYADRDMLDLIIRNLISNAIKFCNAGDKIQIWSSVENSHVKVCVEDTG
                     VGIDAHSLKKLFGSQVSSTPGTRNEKGTGLGLILCREFVEMNNGKIWAESTPGKGSQF
                     FFTLPISGEQASR"
     gene            complement(12640..13527)
                     /locus_tag="PK502_01770"
     CDS             complement(12640..13527)
                     /locus_tag="PK502_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99575.1"
                     /translation="MNTKRLLLISAILFTTILAWIIPVEAQNRPPQNVYRVTVTSRYE
                     IKDGKRTTNSRAIRQEIKDSLGRTHTILNRDYESQRVTSHTWHTFSGKIITRTDEFEN
                     ERLIRFHLFTYTPDSLIASEAIYRVQPGDTSLYLNIRYGYTNRRPVQAIATSATGKKA
                     YTLKSEWDSQGTEIKRTVKSKKGFAPLDSIALLTCHPTYDSLGRKVAETISRKFLDGS
                     SKTQQFRYAYDSKGNRIMIEELNPSGSTIARTEMQYNDKGMLKFISRFDAQNNLIDYQ
                     AIRYELYPSRNRLNMIIEY"
     gene            complement(13698..14549)
                     /gene="nadC"
                     /locus_tag="PK502_01775"
     CDS             complement(13698..14549)
                     /gene="nadC"
                     /locus_tag="PK502_01775"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPG99576.1"
                     /translation="MATNPELLVEALLNLAIAEDIGDGDHSTLSCIPPSATGKVQLMM
                     KQEGIIAGLSVALKIFKKIDSSVEVEFFRGDGDLLRYGDIAFKAEGKVHSLLQAERLV
                     LNVLQRMSGVATQTREYVNRCEGTKAKILDTRKTTPGMRILDKMAVVIGGGMNHRMGL
                     FDMIMLKDNHIDFAGGIENAVALAHKYLNETGRKLAVEVEARSLKDVQVILRVGGVNR
                     IMLDNFSVEATREAVGMIGGRVETESSGGITLENIRDYALCGVDFISVGALTHQIRSI
                     DMNLKKI"
     gene            complement(14550..14975)
                     /locus_tag="PK502_01780"
     CDS             complement(14550..14975)
                     /locus_tag="PK502_01780"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HPG99577.1"
                     /translation="MGKQKIKLLSLFLLIMLGSSASVLQQDSNEIISKSAEALQNGDA
                     VNLAKYFYKTIEIDMLGEENFYSQAQSVQLLKNFFEKNKPVKFAVSHQGAKETSAFAI
                     GQLQTKGNTFRISIFLKSDGSQFFIHQLRIEQDEEGNGI"
     gene            15435..15908
                     /gene="rlmH"
                     /locus_tag="PK502_01785"
     CDS             15435..15908
                     /gene="rlmH"
                     /locus_tag="PK502_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPG99578.1"
                     /translation="MKIHLILIGKTSVPYLSEAMNDYLKRIERYCRFEVKVLPELRNT
                     KSMPVEVQLQKEAEVLMPVMGNYQEVILLDEKGRELSSEEFAGFIQKKQNSGLRDIAF
                     VVGGPYGFSDGVKLAAAERLSLSRMTFSHQLVRLVFVEQLYRAFTIINGEPYHHA"
     gene            15972..19640
                     /locus_tag="PK502_01790"
     CDS             15972..19640
                     /locus_tag="PK502_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG99579.1"
                     /translation="MNKRLVAIVVLAIASLALLLVSQYLSKSALDVRLYRPNVKHLER
                     IVERETTYSGKLAREFLGKVNSNASSRQIFESGVEFKRKLEGKWDGELFLFSHDTLCF
                     WSCKLDVSGVPFDSSQVGLVKIQSEYFFTQWFTAGKGLQLLVLNPIYSEYPYQNKYLK
                     NSFSPLFKEFNNYRISIDLVSNAIPVRPHNVAPFYLVPLDIYQNGHVNRLWPILQWIA
                     FAMAVCAVLLIFGQKFFRYKPLLKIAGATAGIFIIRYLSLYMEIPSRSGMKIFNPEIY
                     AHSFINPSLGDFFINAILVFMLVLFIRQSIRLSLRHIKNMHLYLLAASVLILFLALFP
                     ITDKLLTSLVTHSTLTLEPYRIFSLSVYSIIGYLSVSLWAASAVILTITWVSVVAEQL
                     SLKQRLILLVSGLLVVTLLSLALTSKPSGLGLVWMWLTVAYVLLVYRSSKTHLLLRHI
                     LVIVTIFSAYSVSTVFIKTSEKELNVRKVLAINLSSERDPLAEMLLPNLYQKMLTDSY
                     IRDCIRNIEQKNAELYSYLRKDYFKNYLNRYDLRATVCSPSSIINVDGKSTLLCDRFF
                     GMLIGDYGIMLPSSRFYYLNTQSGFISYVGEINYRIGDNIRNLYIELTSRPGWEMLGY
                     PELLIEGRASQQQLKEYSWAKYHNGNLITQSGEFPYRQKVSGINPTTQVFTCENFLGY
                     NHLTYRPGSSDIVVISRPREKLLNVTASFAYCLFLYFVMFLLCFKIADIPLRLIPTHA
                     SFKNRITRAIILIVTLSLLMVGAATIAYNIKNFEQRNEKNLSEKLISIMFELDRDVDL
                     IQSNDPSAIGFLSDRLVELSNIFYTDINIYDTTGMLLATSRSEIFEKQLVGPRMNPAA
                     WHELATNHSPKFLDNENIGKAKFLSAYVPILSSNREAIAYLNLPYFTRQEELRSELYS
                     IVVAIVNIYALLTLIAISIAIIISSQITRPLQLIRERIGKVSITGNNQTINYSSDDEV
                     GQLIGEYNRMVNELTRSANELARNQRESAWREMAMQVAHEVKNPLTPIKLSLQHLVKA
                     KKDNLPDWDARFERFAQSLLEQIESLAVIASEFSLFAKLPSAKREPVKLLDIINDTAN
                     LYRGYRNVALNVISYMEAEPLVLADKEQLIRVFNNLIKNAIQSIERGKDGKVTITLAP
                     ENDLLIRVDVTDNGVGIPEEVLPKLFTPNFTTKSGGTGLGLAISREIVLGFGGEILVK
                     TQKDLGTTFSVLIPFLKH"
     gene            complement(19658..20953)
                     /locus_tag="PK502_01795"
     CDS             complement(19658..20953)
                     /locus_tag="PK502_01795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG99580.1"
                     /translation="MSKSGLRSFPRNFWTVITMEFFERGSYYSVMSILSVYLVLGVNE
                     GGLGFTKESVGVIKSVITPLLYILPIISGAIADRFGYRKILFFSFAVMSLGYLLTSFS
                     NTYATVFLSLILMVTGAGFFKPIISGTIARSTDSRNSSLGFGIYYWAINLGAFLFPLI
                     FVPLLKSIGWSYIFIMAAVGTGWLLFLNLFAYKEPTIPKSNKSLIMVFKEMVLVLKDY
                     KFILMIVVYSGFWVLYFQMFDSVLWFLKEYVDMTPVDTFVNSLLAIFVSNPNWHFDAE
                     HVTVVNAGTIIALQLVISAIVKNTRALPTMIVGIAMGTIGMAVLATSNYAWVFIGGMI
                     VFSLGEMIAQPKFISYIGLIAPDDKKALYMGYSFLYGVIGSGIGGVLGAYSYVKLVNE
                     MGRPSMFWLMFSFIGVATILGLLAYNRFILKKGTANPHS"
     gene            complement(21170..21706)
                     /locus_tag="PK502_01800"
     CDS             complement(21170..21706)
                     /locus_tag="PK502_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99581.1"
                     /translation="MKKILLACLAAAVFLITCIAASAQEDLKLQKLEEKVKIAQEKLD
                     KADAKVNSADSLISAGKSIIQEAEVEVRALEAEKKQLDKAHFAELKPLEKQLRSKDKD
                     EANSAKEEIKASETKYKEDLKGWNASYKEALKKYEAGKKMVQKGEENMKKAKDARKEA
                     EATLKEAEQALSKAEKAK"
     gene            complement(21825..23804)
                     /locus_tag="PK502_01805"
     CDS             complement(21825..23804)
                     /locus_tag="PK502_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPG99582.1"
                     /translation="MKKFNLYMGAIVAGVLMFYSPARSQEFSKIVPKQLGEKITLEYA
                     ISGETPGQLFNITPYYSVDGGKTFKQPKSVKGNVGSNVPGGRNLIVVWDVLKDLEELN
                     SDVIFKLDAKTSTTKPLTDDFNQVHFSLESLNRTKDGNIELLLSITNNGPTRDLKIIN
                     GLVTITDFNKQKYDAQKGNIAEVQGNERYSTPTRTIKQGETVKSRFVFERIPAEMDRI
                     MRLDLGVELITDEKYGIDLKIGKLQFRDLPVSPAPTTGLKVKRDYSFQLTSQPARFDI
                     RKVEIADAIPPEIVVTSPADVPLAGKEATRGRPYAQSSIGMDDRRLRSLASGEEYATT
                     DSSMTVKGKASDESGIYQVLVNGFEAKVDSNGIFEASIPLEIGRNEIAIRAVDLRQNA
                     VEKKFFVLRKAPSGKVAKGETEELDLVFDQPKTAPKFYALIMGANDYQDNSITDLDNP
                     ISDATKLYNVLVNRYTFDPNNVVFLKDPTREQMINALDMLTRRIGKNDNLLIFYAGHG
                     YWDKETDFGYWIPTDSKGNSTANWLANSQIKDYVAAIKSKHTLLIADACFGGSIFHSR
                     KAFQEGSSSTKEAFDAPSRKAMTSGNLTEVPDRSVFLEQLVTRLDGNTKNYVTAEEIF
                     ASIKNVVMSSSPVTPVYGEIKDAGDEGGDFIFVRK"
     gene            complement(23872..24918)
                     /locus_tag="PK502_01810"
     CDS             complement(23872..24918)
                     /locus_tag="PK502_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99583.1"
                     /translation="MVKFYLSSSLVLIFNMVIPFSGRSILAQDWNKFEGLFIQDTTQK
                     VKDTSNCAFKNISTLSYYPDTLPGWFFSPPVLYQGAAGLGISDPDMPMEKAREQAYLR
                     AKSMALLNYNARVQYYRDIYSSANEVGRYTNLRQRFDTYFKISSLATVSNAMFEVVDT
                     HFTRYNEYVVLLSYNPGNHSDDSLYKLTSVGSAFLVEATFDDVVDEQAEYELLCNATF
                     GEDSAASSHFLFREKGKRFLTSSFMNNAEVGYPMFPYSYVSPQDSARQHPFISYSGLW
                     SSYLRQLVNYLTLNAQQSSIKLKSMDDQYSTKLTNLSREIASFRASLNINGIKFANDT
                     LKLDLQLNEVESNF"
     gene            25290..26207
                     /locus_tag="PK502_01815"
     CDS             25290..26207
                     /locus_tag="PK502_01815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG99584.1"
                     /translation="MISICIPVYNFDVTELVDELLRQANHLNYPVEVLIFDDHSQQYF
                     RQQNSLLASRSKTSYLEFDFNIGRSKIRNRLADFAHGDWLLFLDCDVMVDSPDYLKTY
                     IDNLGRAKVLCGGREYGPKPFRPELLLRWKYGVSRECKSAFVRQTDPYSSFISGNFVI
                     SADIFNAIRFNEALSGYGHEDTLLGIELKRHNIEILHLDNAARHLGLDPCGEFISKTE
                     QGIVNLVRILNISAGNRREIEKSIKLLRIFRFLRHIGMLYPLRWVFKVLTPYIRRQLC
                     SSNPNIQLFDLYKLSLLSKIYRPHWRQVS"
     gene            complement(26246..27553)
                     /gene="der"
                     /locus_tag="PK502_01820"
     CDS             complement(26246..27553)
                     /gene="der"
                     /locus_tag="PK502_01820"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPG99585.1"
                     /translation="MGNIVAIVGRPNVGKSTFFNRMVGSRHAIVDQVSGVTRDRIYGK
                     SDWNGVEFSIIDTGGYVVNSDDVFEEEIRKQVILAIEEADVILFLVDVRDGITSMDTA
                     VADILRRSSKTVHLVVNKVDNNLLRYDANEFYRLGLGTPMCVSAINGAGTGDLLDEVV
                     KSFSPVHEEVQELEIPRIAVVGRPNVGKSSLINSLVGEERNIVTPVAGTTRDAIDTTY
                     NKFGYNFTIVDTAGLRRRTKVEENLEFYSVVRSIRAIENSDVCLLLVDATQGMENQDL
                     FIFNLIQKNRKGLVIVVNKWDLVEKDTKTTDRYTKLIKERIAPFQDVPIVFASALTKQ
                     RILKVLETASNVYKNRKQRIPTSKLNEFLQATLDIQQPPTYKGKYIKIKYVTQLPTPT
                     PAFVFFCNLPQYIREPYKRFIENRLREQFNFTGVPLQIYFRAK"
     gene            complement(27657..28538)
                     /gene="era"
                     /locus_tag="PK502_01825"
     CDS             complement(27657..28538)
                     /gene="era"
                     /locus_tag="PK502_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPG99586.1"
                     /translation="MTHKSGFVNIIGNPNVGKSTLMNALVGEKLSIITSKAQTTRHRL
                     MGIVNGEDFQIVYSDTPGILKPAYKLQQAMMKFVETALEDADIILYVTDTVETPNKNQ
                     EFLNKLQQLGKPIVLAINKVDLTTQEKVQELFNLWSTMMPNATVIPISALHGFNLNSL
                     VDFILDRLPEGPEFFPKDTLTDKTLRFFASEIIREKILNNYRKEIPYSVEVEIEQYTE
                     SDTIDRIKAIIYVERESQKSILIGHQGNMLKKVGTEARLELEAFLGKKVYLELFVKVN
                     ENWRNDTSKLKRFGYPL"
     gene            complement(28623..>29692)
                     /locus_tag="PK502_01830"
     CDS             complement(28623..>29692)
                     /locus_tag="PK502_01830"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004351309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPG99587.1"
                     /translation="PYEDSRPDVHKPFRSSDPYHDDKPKHFEGNRHEFSDGHFSHPKN
                     FRTNSRNFSSDRRERQGSSYNRDFDSNPAQSYRSRTFQGSYGDERKHIERKNLNTFRE
                     RSPQKDNSGEISTNPTGPIRLNRYIAISGLCSRREADVYIQNGQVTVNGKVVTELGTK
                     VNLTDEICFKGKVLKSERMVYILLNKPKDYITTMDDPHAKRTVMELIEGACPQRVYPV
                     GRLDRNSTGVLLLTNDGELTAQLTHPSFKKKKVYQVGLDRKFNPNDFQKLTEGIEIDG
                     EVVKVDAVDYVEGGDGTELGVEIHSGQNRVVRRMFDALGYGVVKLDRVYFAGLTKKNL
                     PRGKWRFLTPKEVNMLKMGRF"
BASE COUNT         7802 a         6577 c         6729 g         8584 t
ORIGIN      
        1 tttagggtag agtttttatt ttcaaagggg ataggcatga tttttttccc gatgctgcaa
       61 tataaaaagc agggagtaga agctgaaata ataaccccgg tgagggttga acaattacct
      121 gctacttttg ttagcgggtt gatgcttttg tgctatttca gagcaaccga aagtaaggat
      181 gttataattt tttcgggaac catgggtgat tacgtacagc ttaggcgtga gtacatgctt
      241 aaatcgctgc ggaaaatgga tgttctacca aatccgtttg aacaatttga tatttggttt
      301 aaggaggcag tgaatgccaa gctacccgat ccaaacgcca taacccttgc tacttcaacc
      361 tttgagggta ggccttcggc acgtgttgtt ttaatgaaac actacgatag cgatggaatt
      421 gcttttttta cgaactacga gagccgcaaa gccaggaacc tgctgaaaaa cccctacgct
      481 gctatggtgt ttttctggcc ggagcttgaa aggcaggtaa gaattgaggg aaaggtgtcc
      541 agggtgagcg agcagcagag cgaccgctac tttcaaactc gtccagaggg aagcaagatt
      601 ggtgcatggg catcgccgca aagttcagta attccgaaca gaaggtacct ggagcagctt
      661 catgctgatt tcatggagga gtttcaagga acaaccatta agcgtccacc aaattggggt
      721 ggctacattc ttcaacccgc tctttttgag ttttggcagg gaagagagaa tcgcctacac
      781 gaccgcatcc agtactccct ggttaacgag aagtggttga ttgagcgctt ggctccctag
      841 ccgagccgtt ggcttttcca ccagtcaatg taaacattac tattatccag cccagccaga
      901 cctggcggtt cggagatatt gcttttgcac ccacctatgc taaattttgt gcgctttggc
      961 aagttgcatt catgcgccaa tgcccccggt tacttttttg tagggcttaa aatggcaaag
     1021 ccttaaaact tcaaccttaa gtgtgaattc tgccacgaag gcttgagata gtgttaggat
     1081 ataagctacc tttgaagcag gttatctgca tttgggcggg taagcgtact gttggataat
     1141 gcccgaagcc gctggcagtg tgaacctgaa atgagacatc ctcattaccg gctatgatgt
     1201 gtggtgagta ctttccctgg attactacct ttttgccaac ccttagcaga gaggaacaaa
     1261 tattttttta aaactataca cctagggtca tagtaaagtg ttttgctagt tgtaataaaa
     1321 agttgaaata tgcatgatga gctggaaata gtggagtaca actttgagtc gtcatcacac
     1381 aagattcttc caggtgtgaa taaatatcat tgcacattgc ataacctaga tattgagtgc
     1441 aactatactt actctaccga ggttggcaat cctaatagca atgttgacac caaggatatt
     1501 tgtattgaag aaaacagatt ttataagtat caaatcataa aacctaaagg cgaagccacc
     1561 acccaaagag tggtttttct atttcatggg ttcaacgaga aagactgggg aaaatatgtg
     1621 ccctgggcca aggcaatttc tgaaagcaca ggattcagcg ttatactatt tcctatagct
     1681 tttcatatgc agcgggcgcc aaaaggctgg agcaataaaa gggagatgtt taagctgagc
     1741 gaaatgagga aaagaagcta cccgaatgtg gtgcactcaa ccctgtctaa tgttgccatt
     1801 agcatccggc tgcattcaat gcctcagcga tttatatggt ccggattgca aacttactat
     1861 gatgtaattc agctgattga agaatgcaag gaaggaaagc acccctacat cagcaaaaat
     1921 tttgaatttg atttttttgc ctactccata ggcggttttc ttgcccaaat acttaagcta
     1981 agcaactaca acaattattt cgctaactca aaggtttgtt tattttgcag cggagctact
     2041 tttaaccggc tatcaccagt ttcaaaattt atactcgata gtgaggccaa catagcgtta
     2101 tactcatttt taatcgaaca ttttgattcc gttctgcaaa aagacaacct gctccatcat
     2161 tatatcactg aacatttaga gggtcaggtg tttcggtcaa tgctcaacta ccagaggatg
     2221 agagatctcc gtgaaggctt attgaaaaaa tacgaagatc agatttatgc tatcacccta
     2281 aaaaaagatc ttgttattcc ctcttttgag gtaattaata cactaaaagg agcctaccgc
     2341 acaattaata ttaaagtaga agaacttgat tttaacaggg agtatattca tgaaaaccct
     2401 tttaatgctg atagtagcgc atcacagctg gttgatgagg attttaagct ggtttttagt
     2461 aaagtttccg atttttttaa aaaaggataa cttcaaaatt tggcttgatt tactttctat
     2521 ctctggtaaa atgtgcccca aaatggacgc tattagattc tttgccgtat ccgttcttca
     2581 gccaaagttt ttctacgaaa agattcattt cccattagcc ttaatgaaac ttaagacatc
     2641 acgccataat tggcctacaa aatatgttag gttcagctgt taagttggat taccggacaa
     2701 gttagggttt ggctgtgttt ccatccctga agccctgcca gatgggggta tttttccatc
     2761 cattcccttg ctatggttga acgcttagtc ccctaatcgg actgctggga tttgctacca
     2821 atcaatctaa gcattttacc aataagcaca gcggaatcta aaggtttggt gatgtagtcg
     2881 tcgcaaccgc tttcaatgct cctgtttagc tccgactgca gggcgtaggc cgtttgggca
     2941 attataggcg tgttgggccg cattcgcttg aactcaacag caagttcaaa accgtcggca
     3001 tcggaaaggc caatgtctag cagaacaagc cggatgcggt tgtttgactt gaatagcttg
     3061 cgtgcctcct tggcattttt ggcaaataga atttttgcac ccgttcgttc cagcacccgt
     3121 tcaaagtaaa gcctgatttc gttaacatcc tcaacaacga gtacagtttt tccttttagc
     3181 tggcttacag gttgtttcac ttcgcttgaa ggttggacgg gggctggcgt atctttttcg
     3241 gaaaattcaa ggggaatgtt aaagaaaaac tctgaacctt caccaggttt actctccacc
     3301 cagatatccc cgcccaggag ttcaacaagg cctcttgaaa ttgccaggcc aagacccgaa
     3361 cccccatata atcgggtgta tgagttgtcg gcctgcctaa aacgctcaaa gatgagaagc
     3421 tgattttcta tagagatgcc tattcctgtg tccctcacgt aaaactgaat ttttgaagcg
     3481 tcatcatccg aaaatatgga gtatccaaag tttatacttc cgcttggggt aaacttaatg
     3541 gcgttgctca gcaggttgga gagaatttgc ttaattctca ccgggtcgga gtatataaca
     3601 aaaggagata cacaggtttt ttcgggcttg aagagcaggg ttttgcactc atccttaagc
     3661 ttaagctggt tggaaaatat caggtaaagc tcatccatca gctcgttcag gtcgaatgcg
     3721 tttttatcaa tccgtagctg gttcgactgg attttagaaa tatcaataat atcctccagc
     3781 agcgacagga gctgcttgcc gctgttctga atcagctcga tgtactgcat tccggtttcc
     3841 gatagctgct catcactttt gattagctgg gtgaacccca gcacagcgtt cattggcgac
     3901 ctaatctcat ggctcatgtt ggagagaaaa gctgttttga gcctgtccga ttcctctgcc
     3961 ttttcctttg ctcggatgag ctcctgctca atcttttttc gctcggtaat gtcgttagca
     4021 attcccacat gggctattgc gattccgttc tcatcgtaaa cggccgaggt ggaaaggaat
     4081 attgggaaaa ctgcgccgtc ttttcgaacg ttgatgagct ctccttgcca tccgccctta
     4141 agggtttcct catgaattgt cttatcaagt gttaagtccc tgtcgggggt tttgaggatg
     4201 gaggtgtgct tgcctataat ttcggattgg gtgtagccat aggttttggt gaaggcgggg
     4261 ttaacgaaaa taaaatgtcc atctgtattg gtaatggaga tgctgtcgtt ggcggtcatg
     4321 atggtttgcg ccagcatgtt tgcttgagct tcaaactgtt ttttctgggt aatgtccctc
     4381 tgtaggacca ggaaaaggct tcgtcccgat tgaagtagcc taacttcgtt gaagtaatcc
     4441 tgattgtttt tggaaaactt atactcaaaa gtctgaagct tgccgctttg ctttgccagg
     4501 ctaatggcat cagtaaattt ttggaaaacg tcctggggta gaaattcgga aagggaatgc
     4561 cctatcattc cagcgttaag ttcaaaatca agcagttgtt ccgaaggggc aaaatcgata
     4621 aggtcaccgt cggcattgat tcggaacagc ctatcgggta tagctgtaac catggcccga
     4681 aaacgctcct cgctttcctt tagagcatcc agcgtgaatt tatgctcggt gatgtcaacc
     4741 agcaccccat cgaggtagtc aacctcgccg gaagcatttt taacccttcg gagttgattg
     4801 ttagcccata aatttatgcc atcttgtcta cggaacgatt gttcggcagg tgcacccggt
     4861 gatagcttta ctgattcttc aacccaatcg tcgtaattag tagctataaa ctcatccatg
     4921 ctgtggccta tcacttcgct ggtatcgtta aaaccaagcg ttttggcaaa ggcgggattt
     4981 ccgtacagta ttttcccttc aggggtcaac cggtaaatgc caacaggtat ttgctcggtt
     5041 agggttcgga acttatcctc actttcacgc aggatagcct cagcattttt tcgctcggtt
     5101 atatcgtgtc caattgccag cacaacctga agcccaaagt aggtgccctt gctaagcact
     5161 atatccattg gcactcgatc gccattgctt ttctttcccc aaaattctaa gtattgagtt
     5221 tgaccctggt gcgctatcct aatcttccgc ttcacctctt ccgtgttgct cttttcctga
     5281 tcggccagct ccattagggt taagcctagg gtttcctcct tttctttacc gaacagctta
     5341 agcccgcttg ggtttacatc aagcagtatg ccgctggcgt caatgatgta aatggcctca
     5401 ccggagttgt tgaaaagccc cctgtagctc tcctcgcttt ctcttatggc cttttcggcc
     5461 tctttccgta gcgaaatatc cctaacgacc accatggtaa ccctttcgtt gccaatagtg
     5521 gccaggttta cgtttagctc aacaggaata ttgctaccat gagcactcac aagtaaggtt
     5581 tcatagattg aaggttcttt tatcccctcc aaccgatttt tataacggtt ttgtaatttt
     5641 tcccattcgc ttttaggtag gtaggtataa aatggcttgc caactagctc gcaatgttga
     5701 acctccagca gttctgcaat gcgtgggttt ccgtaggtta ttatgccatc ctttgagtaa
     5761 attatgccat cgttggattg ttctaccaga attctgtact tctccagcga ctcccccagc
     5821 gcatcttctg ccttttttgc tcttgtcatg tcgatggaca caaccataat gccgaattcg
     5881 ccgctgggaa ataaaactcg ggattcgtgc agctgaacaa atcgcttgct gccatccttg
     5941 cgtaggttgt aaacctcggt gataagctgc tcaccagcaa gtattttttt tatattctga
     6001 tcaacttgag gaagcgtttc tggcggaaca aggtcgtgaa acctcatttt aagtaattgg
     6061 gaggacgaat accccagaat ttcgcttgtt gcattattcg taaaaattat actcccggcg
     6121 ctgtcgctaa gcagaactcc gtttggcgac tgttcaaaaa gtacgcgcca ggcttgttcc
     6181 tcagaaagga tttttttaag cctaacatga ttggatcctg cattgccatc ggagaagatg
     6241 gaggaatgat cgaaccactt ccttgtgatg gccagttcta cttgcaaact cagctcatgg
     6301 ttggtaaaat caggatttac gtggttggtt gagtctagct cgggaataga tttttgagca
     6361 tcggcagtat ttttgccgga gtagaacagc agtggttttt ccgggtattt cctaaaaact
     6421 ttaaggatgg cgttgtagct tgcacgatca gctactccta ctatggccaa gtcgaatgcc
     6481 ggtagctcca catccgagcc aattctatca agggtaataa gggagtaacc catcccatca
     6541 agccgaggct caaattggaa agaaggtaaa actttgttgg ttaccagcag cactattctt
     6601 gaagccataa ccaaaagttt gaaagttgaa atttagcatt tttcaactcc gatttcaact
     6661 ttttttaaca taacatccgt caacccaagg tatattttga cgatggagat taatttgttt
     6721 accagcaacc cgttacagtc tttagatgga aaaggtttgc agctgctata tcaaaagtga
     6781 gccgttttgg gttggtagct ctccatggca tttagaaatg ataaattttg atacacaacc
     6841 ttcctataaa gccatacctg atattcatca aacagcctgt tggctaaaat actttttctt
     6901 aagctttaga gacacgttaa ccagtgaaat caacacgggt acctcaacca gcgggccaat
     6961 aaccgcggca aacgcctcgc ccgagttgat tccaaacaca gcaatagcca ccgcaattgc
     7021 aagttcaaag ttgttgctgg cagcagtgaa cgacagggtt gccgtttgac ggtagctggc
     7081 accaattcgc ctgctcatga agaaggtaat cagaaacatt acaacgaagt atatcatcag
     7141 cgggatggca attcttacca catctaaagg aattttaacg atgtattcac ccttgagcga
     7201 gaacatcacc aggatggtga acagcagggc aatgggtgtt agggggctaa ttttcggaat
     7261 aaagtgggtg ttgtaccatg ctttactttt aatgcgggtg agaacgagtc gtgttaaaaa
     7321 cccggcgatg aagggtattc ccaggtaaat gaacacgcta gtggctattt gtccgatggt
     7381 aatttgtgaa atggtttcgt tcaccggaag cccgaaccag cctggcagta tggcaataaa
     7441 caggtatgcg taaaccgaga agaacagcac ctggaaaacc gagttgaagg ctacaagccc
     7501 ggcagcatac tcagtgtcgc ctccggccag ctcgttccat acaatcacca tggcaatgca
     7561 tcttgcgagc cctatgagga tgaggccata catatagtca atgttgaatc gaaggaatac
     7621 aatggccagc aggaacatca ggatgggccc aataacccag ttttgaatta acgataggga
     7681 taaaaccctg gtgttgcgga aaacatcgcc cagctcctca tacttaacct tggccaaggg
     7741 tggatacatc attagtataa gcccaatggc tattgggata ttggtagtac tttcgggcga
     7801 tgcttttagt gaatcccaaa atactgctat ggatgggaaa aggtagcctg acagcacacc
     7861 aaccagcatg gccaggaaaa tccacaacgt caggtagcgg tcaaagaact ttagtcggct
     7921 tttcgaaggc atggctttaa ggctttaaca ggttatcgtg aaatttccgg aattcttccc
     7981 caatttgatc tctcaccctt cggaataccg tgagcacctc ctcatcgctg ccctgtgctt
     8041 cggcagggtc ttcaaaacca atatgaagcc gatttttgac cttcccaatg aatgcggggc
     8101 aggattctct ggctcctcca caaacggtaa tcacgtagtc ccattcctcg ttaagaaact
     8161 catctacatg atgggttcgg ttatggctga tgtcaattcc cacctccccc attaccttta
     8221 tggcatatga atttacggct ggtgcgggtt gtgttccagc cgaatgcact tcaaaattac
     8281 ctccaaacaa cttgagaaaa ccctccgcca tttggctacg gcagctgttg ccggtgcata
     8341 gaattaatat tttcatggtt attcccgata taagaatgga tggagtgcgt ctaattccgt
     8401 aacctcaaaa aaattatact ctaaaaaaac actaaaaaat gggaaaagtt tagtacacta
     8461 ttttccgggt aattctatgc tccaactttt tgtctttaac attcccgttg ggggtaaccg
     8521 tacactggta ggttagcttt tcagtccagt tcccaaaagt atcatagcta tactcgtagt
     8581 agtagccttg tccaattttt gatccaggca acagctcgag aatgacgtcg ccatgggagt
     8641 taaattctct tttcagggtt gatttaggct cgggtttgtt aggatcgagg gggtatgcgg
     8701 ttgccattac aaactggtcg ttggccttgt aggagagcac cctgataagg ttgttggcag
     8761 gcacatagat gaggcggtct ataccgctca gctcgccctt ttcattgaat accctaacct
     8821 ccacaatccg tccggtgctg tcgcagctta ccagagcagt gtttataatc ttactttcaa
     8881 cacttttgtt aatctcgttc aggtatccgg tggtgtcgtt atatttcagg gtgatttcct
     8941 cgttcaccca acccttgtag ttgatgaatt gatgcgtagt ctttctaatg gggtagccgt
     9001 ttatgttata ctcccacctg acttcattct gaactccccc ggcgctgttg tacttaattt
     9061 ctgatactac cttaccctga gcattaaact gtttcaccac cctgaacgat ggagttttgg
     9121 tttggccaag taccaggact tcgtggagct cttccacgct tttaaatgca tggcctcttg
     9181 attcagaccc cctggagttt aggtaaattt caaccagcgg ctgtgcctgg aggtataagc
     9241 cgagttctga tagtatgcaa aaaagaatta acagagctag tttgcgcatt tttcttctgt
     9301 caatattttg gtaaatttaa taattctgta ttttcggagg cattagctct atttttttct
     9361 gattttgctg gaaagaagta ttggcgttaa agttctacaa ccctaaatat catttgttca
     9421 gcctgcattt gtaaccagta cttttaccta aattgtggat aaccgggagg gagcgctgaa
     9481 attcaaaagg ctggcataac agcgctaagt tttttcgccc tttattaaag ttacctttag
     9541 gctgtgctga aggttaaatt atctggaacg ttgtatttta gactacaatg tgtgtagctt
     9601 tatgactaat tttatgctgg ttaaaaaata aaattccttt aatgtagtta agcatttcat
     9661 gttgaatggg tatgaaaaaa aggttggtag tgttgacagg ggcaggcgtt agcgccgaaa
     9721 gcggtataaa aacgtttcgt gattccgatg ggctctggga ggagtatgat gttatggagg
     9781 ttgccagcat tgaaggatgg tccaggaatc cacagctggt gctcaatttt tacaacgaac
     9841 ggcgcaggca gctgcaaaag gctatgccaa atcctgccca cttgtctctt gccaaggctg
     9901 agtcggactt tttggtttac attatcactc aaaacatcga cgatctgcat gagcgggcag
     9961 ggagcactaa ggtgcttcac ctgcacggtg agctgaaaaa ggctcgctca actgttaatc
    10021 ctgatcttgt atatgacatc gggtacagcg atatacactg gggtgatatg tgtgagctgg
    10081 ggtcacagct gcgtccacat gtggtttggt tcggcgaggc ggtgccggcc atggaggaag
    10141 ctgtgaagct tacccaaacg gcagagctct ttgccgttgt ggggtcatcg ttgaacgttt
    10201 acccggccgc agggctcttg agctatgttc cccgccaatg ccccatcttt ttggttgatc
    10261 caaaggaggt agccaccttc agagacgata tcaccgttat tcgtgaacct gcctcaacgg
    10321 gagttcccaa aatgctcagg ctaatggcct aaaaatcttc atctactcct aaatcagtat
    10381 ttttagcata acccccgatt tccaaccgga agtttttatg attaaaacga gtattgattt
    10441 tgacccagct gctccgttgg catttcatat tttacatttt atcccagaaa acttacgatt
    10501 taagtaaaaa gccgttcatt ttgtatcgga ataaattgaa aaccgtagaa aatatcaaaa
    10561 tctagaacgt taaacatttc gtgttattga tttaatagga gttgcctgaa tagatttata
    10621 cagaacacct tggcgcaaag tttttggagt gttttttgcc tccatttaac tcaaggagca
    10681 ccgtgcaagg taacgtgttg gccacgtcta taaggcgatt gttttttcgg agggtataaa
    10741 aaatcaactt ttacgcaaca tgaaagcagg gtataggggt tttctagcag cagcagtagc
    10801 agttttactc gttagtttgg tttatccttc tggcgttttt tcacaggatt taagccagca
    10861 aatagctaat tacgaacagc ttgccgctga atttgagaag aatggcgatg ccaaccaggc
    10921 agcctactac tttgataagg cagccaacca ggcatggcag gcaagagacc tgaacagggc
    10981 aattgctaat tttgataggg cgttggcaaa catcaagcag gtggggaacg tcaacggcgc
    11041 taaaacaatc tatacaaacc ttgggctaat atactgcgag aagggcaacc acaacgaggc
    11101 ctataacaac tttttggcag cacttgcagc tgccagaagc ttaggccgaa aggacgatat
    11161 tgccaatgcc ctggttaaca tttcaacaga gcagattgaa atgtccgatt ttactggtgc
    11221 acagaagagt cttgatgaag cccaaaggct ggcgcaagaa gttgataatg ccagagcctt
    11281 aaaaaatatt tacttcaact ataataagct ttacgaaaaa cttggcagca aggataaggc
    11341 tgatgagtac ttcaggctct acatgatgct aaataagaaa ttgcaggatg acgagttcaa
    11401 ggcaagacaa atgctcgctc aggccaaggt tgacagcgct ggaagaatta ttaatcagat
    11461 ttcatacgag aaggaatcta ctgctcgtaa gctaatggaa accagccatg agcttcagca
    11521 aaccaccaac atactcaagg agaccgagcg aatttccacc gaacagcgta tgcagataga
    11581 cctgctaaat actcagatga agctccgcga agccgaaatt aggaaccaac atctactcca
    11641 aaggatttac atcgggttga ttattattct ccttatgttt gccgccattt tgttttgggc
    11701 taataaccag aagcaaaaag ccaacaagct tttaagcgag aaaaatgctg aaattttcgc
    11761 ccagaaggag gaaatcagcc ggcaggcgga tgagcttagg gagttgaatg ctttaaagga
    11821 taggctattt tccataattg cgcacgatct ccgaagtcca ttgttttcgc ttatcaccat
    11881 gcttaacatt gccaaggagg gacatttcac cgaggaaagt tttaaggaaa taatcagcga
    11941 gctgtcgtcc aacgtaagcc acacaaccgc cctgctcgaa aacctactta cctgggccaa
    12001 aaaccaaatg cagggaacca aggtgaaacc ggaaagattc ttcctgaacg aggtggttac
    12061 tcccaggctt cagcagctaa acgaggttgc ccttcagaag aatattgtca ttaccaacca
    12121 cgtggatgaa tcaacatcgg tttatgccga tcgcgatatg ctcgacctga ttatccgaaa
    12181 ccttatatca aacgccatca agttctgcaa tgccggagat aaaattcaga tatggagttc
    12241 cgttgaaaat tcccatgtaa aggtatgcgt tgaggacact ggggttggaa ttgatgccca
    12301 tagcctaaaa aaactctttg gcagccaggt tagctcaacc ccgggaaccc gcaacgaaaa
    12361 ggggacaggt cttggcctga tactatgccg ggagtttgtt gaaatgaata atggtaaaat
    12421 ttgggccgaa agtactccgg gaaagggtag ccagttcttc tttactcttc caatatcagg
    12481 ggagcaagcc agccgttagc ccgattatcc gttctgtttt accaacaact atgcttgctg
    12541 gcgggaaggg tgtgtttttg tagctgatta tgctgatcgg ctgcttgcta taagtgcagg
    12601 gcaaatcatt tagctgattc ggttcaaccc ttttggcaat cagtactcaa taatcatgtt
    12661 taaccggttg cgtgaggggt aaagctcgta gcgaatggcc tggtagtcaa taaggttgtt
    12721 ttgtgcatcg aatcttgata tgaactttag catacccttg tcgttatact gcatttcggt
    12781 gcgggcaatc gtgctacctg aagggttaag ctcctcaatc ataatcctat tacctttgga
    12841 gtcataagcg tagcggaatt gctgggtttt agaactccca tccagaaatt ttctggatat
    12901 tgtttcggcc acttttcttc ccagggaatc ataggttggg tggcaggtga gaagggcgat
    12961 tgaatccagt ggggcaaaac ctttcttcga ttttaccgtt cgcttgatct cggttccttg
    13021 actgtcccat tccgatttta gggtgtaggc ttttttaccg gtagcggagg tggcaatggc
    13081 ctgaacaggc ctcctattgg tgtagccata cctgatgttg aggtaaagcg aggtatcgcc
    13141 gggttgaacc ctgtaaatag cctcactggc aataagcgag tcgggagtgt aggtaaaaag
    13201 gtgaaacctg attaaccgtt cgttctcgaa ttcgtcggta cgggtaatta tttttccgga
    13261 aaatgtatgc catgtatggc tggtaacgcg ctgactttcg taatcgcgat taagaattgt
    13321 atgggttctt ccaagcgagt cctttatttc ttgccgtata gccctgctgt tggtggtgcg
    13381 cttaccatct ttaatctcgt aacggctggt aacggtaacc ctgtacacat tttgcggggg
    13441 acgattttgg gcttcaacag gtattatcca cgccagaatg gttgtaaata atatggctga
    13501 aatcagcagt agccgttttg tattcatatc tatagaagtt aggcagtaga aattttcctt
    13561 gtaaaaatac gagttaaaag cgaaaaatgt tcaagttcaa gcttttcctt tagctgatgc
    13621 ttaagtagaa aacgtgtggg gttggagggt tgcgaaaaag attgctcagc tattacacgt
    13681 aaagcggaag cagcgattca gatttttttg aggttcatat caatactcct tatctggtgg
    13741 gtaagtgctc caacagatat aaagtcaacg ccgcaaaggg cgtagtctct gatattttcc
    13801 agcgtaattc ctccggacga ttcggtttcc accctgccac ctatcatccc tactgcttcg
    13861 cgtgtggcct caacgctgaa gttatcgagc ataatccggt ttaccccgcc tacccgtaaa
    13921 atgacctgta catccttgag ggagcgcgct tcaacctcaa ctgccagctt tcggccagtt
    13981 tcattcaggt atttatgggc caacgcaacg gcattttcta ttcccccagc aaaatcaata
    14041 tggttatcct ttagcatgat catgtcgaaa aggcccatgc ggtggttcat ccctccacca
    14101 attactactg ccattttatc caaaatcctc atgcccggtg ttgttttgcg tgtgtcgagg
    14161 atttttgctt ttgttccctc gcagcggtta acgtactccc gtgtttgggt agcaacaccg
    14221 ctcattcgct gaaggacgtt tagaaccaag cgttcggcct gtaacaggga gtgaactttt
    14281 ccttccgcct taaaggcgat atctccatat ctgagcaaat caccatcgcc tctaaaaaat
    14341 tctacttcaa ccgagctgtc aatcttcttg aaaattttca gagcaaccga tagtccggca
    14401 ataatgccct cctgtttcat cattagctgt actttgccgg tggccgacgg gggaatacac
    14461 gaaagcgtgg agtgatcgcc atcacctata tcctcagcaa ttgccaggtt gagaagggct
    14521 tcaacgagaa gttcagggtt tgtagccatt tatattccgt ttccttcctc gtcctgctca
    14581 atgcggagct gatgaatgaa aaattggcta ccatcggatt ttaagaagat ggagatgcga
    14641 aatgtattgc ctttggtctg cagctgccca atggcgaaag ccgaggtttc ctttgctcct
    14701 tgatggctaa cggcaaactt aacgggtttg tttttctcga agaaattctt gagcagctga
    14761 acggattgcg cctgactgta aaagttttcc tcacccagca tgtcaatttc tatggtcttg
    14821 tagaaatatt ttgccagatt aaccgcatcg ccattttgca aggcttctgc tgatttacta
    14881 ataatttcgt tggagtcctg ttgaagcaca gaggcactcg aacccagcat aatcaacagg
    14941 aacagagaaa gcagcttaat tttttgtttc cccatttcta cagttttctt attgttacca
    15001 cacggctaaa ctacaaaaat cctcaaaaat tgagccaata acctatagat taatttattc
    15061 tttataccta aggccaaata tacctataat cattttttga accaagtgtc gttgtagaac
    15121 atgtttgttt ccaattatct ccatcagtac ggaagctttt atgagtcaat gtccgacact
    15181 cttcaagccg taaattattt ttttgaattt tttgtttact ccatggtgca gctttgggtg
    15241 taagtatttt ttacctttgc gttagtagta aaatttgaaa taggtgtttt tgctgggtta
    15301 aagtaccggc aggatgttcc cattttttag caggtttgat agcacttaat taaggttgat
    15361 atttttttat aaattttacc atgttaagca ttacgtattt aaaagctgga taaatcactt
    15421 caaccatttt ttttgtgaaa attcacttga ttcttatagg gaaaaccagc gtaccgtatc
    15481 tttccgaagc aatgaacgat tacctaaagc ggatagaaag gtactgccgc ttcgaagtta
    15541 aagttttgcc cgagctacgt aataccaaat caatgccggt ggaagttcag ctacaaaagg
    15601 aagcagaagt gcttatgccg gtaatgggga actaccagga ggttattctg cttgatgaga
    15661 agggtagaga gttatcatcc gaagagtttg ccggttttat tcaaaagaag caaaattcgg
    15721 gtttgcgtga tattgctttt gtggttggcg gcccttacgg tttttcagat ggggtgaagc
    15781 tggctgctgc cgaaaggtta tcactatcaa gaatgacatt ttcgcaccag cttgttcggt
    15841 tggtattcgt ggagcagctc tatcgtgctt ttaccattat taatggtgaa ccttaccatc
    15901 acgcctgatt taggcctgat ttttgatatg aatttagcta catacctaat ccatcccgaa
    15961 ctctcatcga aatgaacaag aggctggttg ccattgtcgt ccttgccatt gcttcgcttg
    16021 cacttttgct ggtctcccaa tacctgtcga agtctgcgct ggatgtaagg ttgtataggc
    16081 ccaacgttaa gcatttggag agaatagttg aaagggaaac aacctattcc ggcaagcttg
    16141 cccgagagtt tctgggtaag gttaatagca acgccagctc cagacaaata ttcgaatcgg
    16201 gagttgaatt taagaggaag cttgagggta agtgggatgg agagctattc cttttttctc
    16261 acgatacgct ctgcttttgg tcgtgcaagc tggatgtctc tggcgttccg tttgattcct
    16321 cacaggtagg tctggtaaaa attcaaagcg agtacttctt cacgcaatgg tttacagcag
    16381 gtaaggggct gcagctgctt gtgttaaacc caatttatag cgaatatccc taccaaaaca
    16441 agtacttgaa aaactccttt agcccgttgt ttaaggaatt taataattat agaatttcca
    16501 ttgacctggt ttccaacgca atacccgtaa gaccccataa cgttgcaccc ttttaccttg
    16561 tccctctgga catttaccaa aatggccatg tgaatcgttt atggcccatt ctccagtgga
    16621 tagcttttgc aatggcagtt tgcgcagtac tgctcatctt cggacaaaag ttttttaggt
    16681 ataagccatt gttgaagata gccggggcaa cggccggtat ttttataatc cgatacctgt
    16741 ccctttacat ggaaattccc tccaggtcgg gaatgaaaat atttaacccc gaaatctacg
    16801 cccattcatt catcaaccca tctcttggcg atttttttat aaatgctatt ctagtcttta
    16861 tgcttgttct cttcataaga caaagtattc gattgtcatt gaggcatatc aagaacatgc
    16921 acctatacct gcttgcagct tctgtgctga ttcttttttt agctctattc cccataacgg
    16981 acaagctgct tacatcgctg gttacccatt caaccttaac ccttgaaccc taccgtatat
    17041 ttagcctttc ggtttactca ataattggat acctatctgt tagcctttgg gctgcctctg
    17101 cggtcatact taccattacc tgggttagcg tagttgcgga acagctcagc ttaaagcaaa
    17161 ggctaatcct actggtatca ggactcttag tggtaacgct cctatcgctg gcactaacca
    17221 gcaaaccatc cggtttgggg ttggtttgga tgtggttgac ggtcgcatac gttctcctcg
    17281 tttaccgcag ctcaaaaacc catctcttac taagacacat tttggtgatt gttaccatat
    17341 tttcagctta ttccgtatca accgtattta tcaaaacatc ggaaaaagag ctgaatgtgc
    17401 gtaaagttct tgccataaac cttagctcgg agcgtgatcc gctagccgaa atgcttttac
    17461 ccaacctcta ccaaaaaatg cttaccgatt cttacatccg ggattgcatt aggaatattg
    17521 agcagaaaaa tgccgagctc tacagctacc tacgtaagga ctactttaag aactacctga
    17581 accggtatga cctgcgggca actgtttgtt cgccatcatc aattataaat gttgatggaa
    17641 aatccacctt gctttgcgac aggttttttg ggatgctgat tggtgattac ggcattatgc
    17701 taccatcatc acgattttac tacctgaaca cgcaaagcgg gtttataagc tatgttgggg
    17761 aaattaacta ccgaataggt gacaacattc gtaatctata cattgaactt accagcagac
    17821 cgggatggga gatgctgggc tatccggagt tgcttattga gggcagagcc tcccagcagc
    17881 agcttaagga gtactcctgg gccaagtacc ataacggaaa tcttataacc caatcgggtg
    17941 aatttcccta ccgccaaaaa gtaagcggta tcaaccccac aactcaagtt tttacctgtg
    18001 agaacttcct cggctacaac catctcacct atcgtcctgg tagttcggat atagttgtca
    18061 ttagccgtcc cagggagaaa ctcctaaacg taaccgcttc ttttgcctac tgcctttttt
    18121 tatactttgt tatgttttta ctatgtttta aaatagccga cattcccctg cggttaatac
    18181 ctactcatgc cagctttaag aaccggatta cccgggcaat tatccttatt gttaccttgt
    18241 cgctcttgat ggtgggtgcg gcaaccattg cttacaatat caagaacttt gaacagcgca
    18301 acgaaaagaa tcttagcgaa aagcttattt ccatcatgtt tgagctggat agagatgttg
    18361 acctcattca aagcaacgat ccctcggcca ttggatttct aagcgatcgc ctggttgagc
    18421 tatcgaacat tttctacacc gatatcaata tttacgacac aaccggaatg ctgctggcta
    18481 catcgcgttc cgaaatattt gaaaagcagc tggttggacc aagaatgaac cctgcggcat
    18541 ggcatgagct ggcaaccaac cacagcccga aattccttga taacgaaaat ataggaaagg
    18601 ctaagtttct ttcggcttac gttccgatac taagttcgaa tcgtgaggca atcgcctacc
    18661 ttaacctacc ctactttaca cgccaggagg aactacgctc cgagctctac tccattgtgg
    18721 tggcgattgt caacatttac gctctcctta ctcttatagc aatttccatt gccattatca
    18781 tttcgagcca gataacacgt cccctacagc ttattcgaga gcgtataggc aaagtaagca
    18841 ttacagggaa caaccaaacc attaactaca gtagcgatga tgaggttggc cagctaattg
    18901 gagagtacaa ccgcatggtt aacgagctta cccgcagcgc caatgagctg gctaggaacc
    18961 agcgtgagag tgcatggcgc gagatggcca tgcaggttgc tcacgaggtg aaaaacccct
    19021 taactccaat aaagttaagc ctacaacatc ttgtgaaggc caaaaaggat aacttgcccg
    19081 actgggatgc ccgattcgag cgttttgccc aatcactttt ggagcagata gaatctctgg
    19141 cggtgatagc aagcgagttt tccctatttg ccaagcttcc ttctgcaaaa agggagccgg
    19201 taaagctgct ggatataata aacgacactg ccaaccttta caggggatat aggaatgttg
    19261 cgctcaacgt tattagctac atggaggctg aaccgctggt gctggccgac aaggagcagc
    19321 ttatcagggt ttttaacaac ctcattaaaa acgccattca gtccattgag cggggtaagg
    19381 atggaaaagt taccataacc cttgcccctg aaaatgattt gttgataagg gttgatgtta
    19441 ccgacaatgg ggtgggaatt cccgaagagg tgctacccaa gctgttcaca cctaatttta
    19501 ccacaaagtc cgggggaact ggtcttggcc tggccatttc gcgggagatt gtattgggct
    19561 ttgggggaga aatactagtc aaaacccaaa aggatttagg cacaaccttt tcagttttga
    19621 taccgttttt aaagcattag cttctgtttt tgaattatca gctatgcgga ttggcagttc
    19681 ccttttttag aataaaccta ttgtaggcca gcaagcccaa aattgttgcc acgcctataa
    19741 agctaaacat cagccaaaac attgagggcc gacccatttc gttcactagc ttaacatacg
    19801 aataagcgcc tagcacgccc ccaattccac tgccgataac tccatagagg aacgagtagc
    19861 ccatgtagag tgctttcttg tcatcgggtg caattagccc gatgtaggag ataaacttgg
    19921 gttgggcaat catctccccg agcgaaaaga ctatcattcc tccaataaaa acccaggcat
    19981 aatttgacgt tgcaagaacg gccatgccta ttgttcccat ggcaatcccc acaatcatgg
    20041 taggtaatgc ccgggtgttt tttacaatag ccgaaataac cagctgcagg gcaattatgg
    20101 ttccggcatt tacaacggtt acatgttctg catcgaaatg ccaattgggg ttgcttacaa
    20161 aaatggcaag caatgagttt acgaaagtat caactggcgt catgtcaacg tactctttta
    20221 gaaaccaaag aaccgagtca aacatctgga agtagagaac ccaaaagccc gagtaaacca
    20281 ctatcataag gataaacttg tagtccttta aaaccagcac catttccttg aataccatta
    20341 tcaggctttt gttactcttt ggtatggttg gctccttata agcgaagagg ttgagaaaga
    20401 gcaaccatcc cgttcccacg gcagccatta tgaaaatgta gctccagcct attgatttaa
    20461 gcaaagggac aaaaatcagg ggaaaaagga atgctcccag gttgatggcc caatagtata
    20521 ttccaaaccc caacgacgag ttccggctgt cggttgatcg agctatggtt cctgaaatga
    20581 taggcttaaa aaacccggct ccggtaacca ttagaattaa gctaaggaaa acggtagcat
    20641 aagtattgct gaagctggta agcaggtaac caagactcat cactgcaaat gaaaaaaaga
    20701 gtattttcct gtatccaaac ctatcggcaa tggcgcccga gattattggc aggatgtaga
    20761 gcaggggggt aataacgctt ttaatgactc caacgctttc cttggtgaaa ccaagccctc
    20821 cctcgttaac cccaagaaca aggtaaacgg aaagtattga catcacgctg tagtaggagc
    20881 cccgctcaaa aaactccatg gtgattactg tccagaaatt gcgtgggaat gaacgtaatc
    20941 ctgatttact cataaaatat ggtattaaag aagaaaaatc gaaatgttat gggggtgaaa
    21001 gtagtaaaaa aataactgga taagctaatc gtatgggctt attcccggat ttcaggcata
    21061 aaaaaaaggg accctcctac gggtccctcc actggtgtgt ttatagacga aacaaactat
    21121 agagccttta taaataggat taaaaatcaa atacaggagt tgtaaagggc tacttcgctt
    21181 tttctgcctt ggaaagggcc tgttctgctt cctttagggt tgcttctgct tcctttcgag
    21241 catcctttgc cttcttcatg ttttcttccc ccttctgaac catcttcttt ccagcttcat
    21301 acttttttaa agcttccttg tagctcgcat tccagccttt caggtcttcc ttatacttgg
    21361 tttctgaagc tttaatttcc tccttggcgc tatttgcctc gtccttatcc tttgatcgaa
    21421 gctgtttttc aaggggttta agctcggcaa aatgggcctt atcgagttgt ttcttttcag
    21481 cttcaagagc tctaacctca acctcggcct cctggatgat gctcttaccc gcgctaatca
    21541 ggctgtcagc agagttgacc tttgcatcgg ccttatcgag tttttcctgg gcaatcttga
    21601 ctttttcctc cagcttctgg agttttaaat cttcctgtgc agatgctgca atgcaggtta
    21661 ttaagaatac tgcagcagcc aaacaagcga gtagtatttt cttcatagga gtaaaatttt
    21721 gctagtgttc ggtaaatgtt taccagctat aactacgaaa ttttatgttt agagtttcgg
    21781 taaaaaacat taagggctac accatgttcg ggtagccctg atgcttactt tcttacaaat
    21841 ataaaatcac caccttcgtc acctgcatcc tttatttctc catataccgg tgttaccgga
    21901 ctgctgctca tgacgacatt ctttatgctg gcaaaaattt cctcggcagt tacgtagttc
    21961 ttggtgtttc catcaagtcg ggtaaccagc tgttcgagga acacgctcct gtcgggaact
    22021 tcggtaaggt ttccgctggt catagcctta cggctgggcg catcaaaagc ttccttggtt
    22081 gagctagaac cctcctgaaa cgccttgcgg ctatggaaaa tgctaccacc aaagcaggca
    22141 tctgcaataa ggagggtgtg tttcgatttt atagctgcaa cgtaatcttt tatttggctg
    22201 ttggccagcc agttggccgt gctattcccc tttgaatccg tgggtatcca gtagccaaaa
    22261 tcagtttcct tgtcccagta tccgtgtccg gcgtagaaaa ttagcaggtt gtcattctta
    22321 ccaattcgcc tggtgagcat atcaagggca ttaatcatct gctcacgcgt tgggtcttta
    22381 aggaaaacaa cgttgttggg atcgaaggtg tagcggttta ccagcacgtt gtaaagttta
    22441 gtggcatcac taataggatt atccaggtcg gtaatgctgt tgtcctggta gtcgtttgcg
    22501 cccataatca aggcgtagaa cttaggtgca gtctttggtt ggtcaaaaac caagtcaagt
    22561 tcttcggttt ctcccttggc tacctttcct gaaggggctt tccgtagaac gaaaaatttc
    22621 ttttcaacgg cattctgtct gagatcaacc gcgcgtatgg caatttcgtt gcggccaatt
    22681 tccaggggta tgctagcctc gaaaattccg ttggagtcaa cctttgcctc aaaaccgtta
    22741 accagcacct ggtaaattcc gctttcatcc gatgccttgc cctttacggt catgctgctg
    22801 tcggtggtgg catactcttc acctgaagcc aatgatctaa gccgtcggtc atccattcca
    22861 atggatgact gggcatacgg acgaccccgg gtggcttcct tgccggccaa cggaacatct
    22921 gcgggggagg taaccacaat ttctggagga atagcatcgg caatttcaac cttacgaata
    22981 tcgaaacgtg ccggttggga ggtcagctga aagctgtaat cccgcttaac cttaaggcct
    23041 gtggttgggg ctggcgatac ggggaggtcg cggaactgga gctttccaat ctttaagtct
    23101 attccatact tttcgtcggt aataagctca acgcccaggt cgagtctcat aatcctatcc
    23161 atttcggcag gaatgcgctc aaaaacaaaa cgcgatttta cagtttcgcc ctgtttgatt
    23221 gtacgggttg gggttgagta ccgctcgttg ccctgtacct cagcaatgtt ccctttttgg
    23281 gcgtcgtact tttgcttgtt gaagtcggta atggtaacaa gcccattgat aattttaagg
    23341 tcgcgggttg gtccgttgtt ggtaattgag agtagtagct cgatgttacc atccttggtg
    23401 cggttcagac tttcaagcga gaagtgcacc tggttgaagt cgtcggtcag tggtttggtg
    23461 gtgctggttt ttgcatcaag cttaaaaatc acatcggagt tgagctcttc caaatcctta
    23521 agaacatccc aaaccacaat gaggtttctt ccaccgggga cattgctacc gacgttaccc
    23581 ttgacacttt tgggctgctt gaaggttttg cccccatcaa cggagtagta tggggtaatg
    23641 ttaaatagct gaccaggggt ttcgccggaa atggcgtact ccagggttat tttctcaccc
    23701 agctgctttg ggacaatttt gctgaactcc tgcgagcgag ccggcgagta gaacatgaga
    23761 actccggcta cgatagctcc catgtagagg ttaaattttt tcatcacaac aggtagttaa
    23821 tatgatacaa tttactttaa aaatttttaa tccgaaaggt gaaaatcaaa gctaaaaatt
    23881 tgattcgact tcattaagct gcaaatcaag ttttagtgta tcattagcaa atttaatgcc
    23941 gtttatgttg agcgaagccc tgaaggaggc aatctccctg ctaaggttgg taagcttggt
    24001 gctgtactgg tcgtccatgc tttttagttt aatgctgctc tgctgggcgt taagggttaa
    24061 atagttaaca agttgcctca ggtagctgct ccaaaggcca gaataggaaa taaaggggtg
    24121 ttgccgggcg gagtcctgag gagatacata agagtaggga aacatggggt aacctacctc
    24181 tgcattgttc atgaaggaac tggttaaaaa tcttttgccc ttttcccgga aaaggaaatg
    24241 ggagctagct gcggagtcct ctccgaaggt tgcattgcag agaagctcat actctgcctg
    24301 ttcgtcaact acgtcatcaa aggttgcctc aaccaggaat gcagagccaa cgcttgtaag
    24361 cttgtaaagg ctatcatccg aatggttccc ggggttgtag cttagcaata ccacatactc
    24421 gttgtaccgg gtgaaatggg tatcaactac ctcaaacatg gcgttactaa ccgtggccaa
    24481 cgatgaaatt ttgaagtatg tatcgaaccg ttggcgaaga ttggtgtacc tgcccacctc
    24541 gttggccgat gagtatatat ccctgtagta ctgaaccctg gcgttgtaat ttaacagtgc
    24601 cattgatttt gccctgaggt aagcctgctc tcttgctttc tccattggca tgtcgggatc
    24661 ggatattccc aaacccgcag ctccttggta cagaacagga gggctaaaaa accatccggg
    24721 aagggtgtcg gggtagtagc taagggtgct gatattttta aatgcacaat ttgaagtgtc
    24781 cttaaccttc tgcgtggtgt cctgaatgaa taatccctca aacttattcc agtcctgggc
    24841 aagaatactc cttcctgaaa atggaattac catgttgaaa atcagcacca ggctgcttga
    24901 taggtaaaat ttaaccatac tccactatag gttttaaata aaacacagaa atctagccct
    24961 aaaaattaag tatttaagat aattttttac ctaaaaggtg ttttgttccg tgaaccatca
    25021 atttacattg atatatccga attgctttct gctccatttt gccattcatt ttgaagtgct
    25081 gtgccagtga cttttgtgca ctgctctttt gatatggagg atgcgacatt tggtcaaaac
    25141 aagcatacat aagctgttaa caatagaatt tactgtcctt aggtatagaa agaaatttag
    25201 ccgaattatt ggaattggta ccccaactct ccttattttt ttattaattg cgttcaaaat
    25261 tatggtgttg ctggttaagc taaacgatca tgatttccat ttgcattccg gtatataatt
    25321 ttgatgtaac cgagctggtt gatgagctgc tccgacaggc aaaccatctc aactacccgg
    25381 tggaggtgtt aattttcgac gaccattcgc agcagtactt ccgccagcag aattcactgc
    25441 tggcctctcg tagcaaaacc agctatttgg aattcgattt caacataggc aggtcgaaaa
    25501 taaggaatcg gttggcggac ttcgcccacg gagactggct gttatttctc gattgcgatg
    25561 ttatggttga tagccccgat tacctaaaaa cctatattga taaccttggc agggcaaagg
    25621 ttctttgtgg tggtcgggag tatggcccca agccattcag accggaactt cttctccggt
    25681 ggaagtatgg cgtcagcagg gagtgcaaat cggcctttgt tcggcaaacc gatccgtaca
    25741 gctcatttat tagcggaaat tttgtaatat cagctgatat ttttaacgcc ataaggttca
    25801 acgaggcgtt gtcgggctac ggacatgagg acacgctgct gggcattgaa ctcaaaaggc
    25861 ataacattga aattcttcat ctcgataacg ctgctcggca cctggggctc gatccatgcg
    25921 gcgagtttat cagcaaaacg gaacagggaa tagttaacct ggttaggata cttaatattt
    25981 cagcagggaa ccgtagggaa attgaaaaat ccataaagct gctcaggata tttaggtttt
    26041 tgcgccacat aggcatgctt tacccattaa gatgggtttt taaggtgctc acaccctaca
    26101 tccgcaggca gctgtgcagc agcaacccca acattcagct gtttgacctt tacaagctat
    26161 cgctgctatc caaaatttac aggccgcatt ggcggcaggt ttcctagata ttccttcttc
    26221 ggctatccct ataactccgc aaggcttact ttgctctgaa gtagatttgc aggggaacac
    26281 cggtgaaatt aaactgttcg cgaagcctgt tttctataaa tcgcttgtag ggttcgcgga
    26341 tgtactgggg aaggttacaa aagaatacaa aagctggagt tggcgtaggc agctgggtaa
    26401 catactttat tttgatgtac ttgcccttgt aggtcggtgg ctgctgaatg tcgagagttg
    26461 cctgaaggaa ttcgttaagt tttgaagttg gaatgcgctg tttacgattt ttataaacat
    26521 ttgatgccgt ttcgagcacc ttaagtattc tttgtttggt gagggctgag gcaaaaacaa
    26581 tggggacgtc ctggaacggg gctatccgct ctttaattag cttggtgtac cgatctgttg
    26641 ttttggtgtc cttttccacc aggtcccatt tgttcaccac aatcaccaaa cccttgcggt
    26701 tcttctgaat gaggttgaat atgaacaggt cttgattttc cataccctga gtggcgtcaa
    26761 ccaggagcag gcaaacatcg gagttttcaa ttgccctgat ggatcgaaca accgagtaga
    26821 actccaggtt ttcctcaacc ttcgtccgtc ggcgcaggcc tgccgtgtca acaatagtga
    26881 agttatagcc aaacttgttg taggtagtat cgatggcatc gcgggtggtg ccggcaacag
    26941 gggtaacaat gtttctttcc tcgccaacca aggagttaat aagcgaagat ttgcccacat
    27001 ttggccgacc aacaactgct attcgcggta tttccaactc ctgaacctcc tcatgaaccg
    27061 gagaaaaact ttttaccacc tcgtcgagca aatcacctgt tccggcgcca ttgattgccg
    27121 aaacgcacat gggggtcccc agcccaagtc tgtaaaactc gttggcatca tacctcaaca
    27181 ggttattgtc aaccttgttg acaaccaggt gaacggtttt tgaagaacga cggagaatgt
    27241 cggcaacagc ggtatccatg ctggttattc catcgcgcac atcaaccaga aacagaatta
    27301 catcggcctc ctcgatggcc aatatcacct gcttgcgaat ttcctcctca aaaacatcat
    27361 cggagttaac cacgtaacct cctgtgtcga ttatcgaaaa ttctacccca ttccaatccg
    27421 acttgccgta aatcctgtcg cgggttacgc ccgacacctg gtcaacaatt gcatgcctgg
    27481 agcccaccat acggttaaaa aaggttgact tgcccacgtt cgggcgacca actatggcta
    27541 ctatatttcc cactgaacgt tcaattttac gttaattatg ttttggttta gcaaaacttc
    27601 ttatctgcag ggagagttaa ggttcgggaa agcttgttat gatgggtaaa acctagctat
    27661 aagggatagc caaatctttt cagcttgctc gtatcgttgc gccagttctc gtttaccttg
    27721 acaaacaact caaggtaaac cttttttcct aggaaagcct ccagctccag ccttgcctcg
    27781 gttccaacct ttttgagcat gtttccctga tgaccgataa gtattgattt ttgtgactcg
    27841 cgttccacgt atataatggc cttaatacgg tcaatggtat cgctctcggt gtactgctca
    27901 atttccacct caacggagta gggaatttct ttgcggtagt tgttaagaat tttttccctg
    27961 ataatctctg aggcgaagaa ccttaaggtt ttgtcggtga gggtatcctt tggaaagaat
    28021 tcgggacctt cgggcaacct gtcgagaatg aagtccacca gcgagttgag gttaaagcca
    28081 tgcagggcgg atatggggat gacggtggcg ttgggcatca tggtgctcca gaggttgaaa
    28141 agctcctgaa ccttttcctg ggtagtaagg tcaaccttgt tgattgcaag gacaatcggt
    28201 ttgccaagtt gctgcagctt gttcaaaaac tcctgattct tattcggagt ttcaacggta
    28261 tcggttacgt agagaatgat atcggcatcc tcaagggctg tttccacgaa tttcatcatg
    28321 gcctgctgaa gcttgtaggc aggttttagt attcctgggg tatcggaata aacaatctga
    28381 aagtcttcgc cgttaacaat tcccatcaac ctatgcctgg ttgtttgggc tttactagta
    28441 ataatggata gcttctcacc cacaagcgca ttcatcaggg ttgattttcc gacatttgga
    28501 ttaccaataa tatttacaaa gcccgacttg tgtgtcatga gcaattcctg attttatgag
    28561 tgttttggct cacgttaacg tgtaaggtag taaaggctac ttttctgaac aggagggggg
    28621 ggttagaacc tccccatttt cagcatgtta acttcctttg gcgtaaggaa acgccattta
    28681 ccgcgtggta gattcttttt ggtaaggccg gcaaagtaaa ccctgtccag cttaactacc
    28741 ccgtagccca aagcgtcgaa catgcgccga acaacccggt tctgtccgct gtgaatttca
    28801 actcctagct cggtgccatc gcctccctca acgtagtcaa cggcgtcaac ctttaccact
    28861 tcaccgtcaa tttcaatgcc ttcggttagt ttttgaaaat cgttggggtt aaacttacgg
    28921 tcgaggccaa cctggtacac ctttttcttt ttaaagcttg ggtgggtgag ctgtgcggta
    28981 agttcgccat cgttggttag caggagcacg ccggtgctgt tcctgtcgag acgtccaacc
    29041 gggtaaaccc tttgaggaca ggctccttca ataagctcca ttaccgtacg cttggcgtgt
    29101 gggtcatcca ttgtggtgat gtaatctttg ggtttgttga gcaggatgta caccatgcgc
    29161 tccgatttca gaaccttacc cttaaagcaa atctcatcgg ttaggttcac cttggtgccc
    29221 agctcggtta ccaccttgcc gttaacggta acctgtccgt tttgaatgta aacatcggct
    29281 tcccgtcggc tgcataatcc cgaaatggca atatagcggt taagcctgat ggggccagta
    29341 ggattggtgg aaatttcgcc ggaattatct ttttgggggc ttctctccct aaaggtatta
    29401 agatttttac gttctatgtg cttgcgctca tctccgtagc tgccttgaaa tgttcttgaa
    29461 cggtagcttt gagcaggatt gctatcgaaa tccctgttgt agctggagcc ctgcctttcc
    29521 ctcctatcgg atgaaaagtt acggctgttg gttctgaagt ttttggggtg ggagaagtgt
    29581 ccgtcagaaa attcgtgcct gttaccctcg aaatgtttag gtttatcatc gtggtagggg
    29641 tctgaactcc tgaatggctt atgaacatca ggacggctat cctcgtatgg tt
//
LOCUS       DAOPHX010000012        29583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_1528_length_29583_cov_5.963221, whole genome shotgun sequence.
ACCESSION   DAOPHX010000012 DAOPHX010000000
VERSION     DAOPHX010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 29583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29583
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1528_length_29583_cov_5.963221"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PK502_01835"
     CDS             complement(<1..849)
                     /locus_tag="PK502_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HPG99588.1"
                     /translation="MISTNIQIAAYLFASILFILSLSGLSSQESAKKGVIYGIVGMAI
                     ALCATLFGGNIQGYVYIIVAMVVASVIGVIVARKVQMTAMPQLIALLHSFVGLAAVLV
                     GFSSFIDPKTVSMAGAVRAIHMVEIFVGIFIGAITFTGSIIAWGKLTENITSKPLVFK
                     GKDVVNVVLLLASVVLGVMFVGQGGTHGMPFLIAMTVIAAFFGVMLVMAIGGADMPVV
                     ISMLNSLSGWTAAASGFMLGNDLLIVTGALVGSSGAILSITMCRAMNRSFFAVISGGF
                     GQEVKTG"
     gene            complement(857..2398)
                     /locus_tag="PK502_01840"
     CDS             complement(857..2398)
                     /locus_tag="PK502_01840"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HPG99589.1"
                     /translation="MNVGIPKELLQHELRVAATPKTVVRLKKQGFEVLVESGCGVRAK
                     FLDEEYVAAGAEVVSSAQELYSSSDIILKVQPPTEAEVGLMKRGTLLLSYLFPAQNLP
                     LLGKLAEAGINAIAMDAIPRISRAQKMDVLSSMANISGYRAVVEAANYFGRFLNGQIT
                     AAGKVEPAKVLVIGTGVAGLAAIGTAKSLGAIVRAFDTRKEAREQIQSMGAQFITVEI
                     DEDGSTASGYSKEMSQAYIDAEMALFKRQAAEVDIIITTAQIPGKAAPKLLLEEHVMA
                     MKPGSVIIDLAAPTGGNCVLTKKDEVYTTPNGVVIAGRVDTLPTQASFLYGNNLCNLL
                     DDMGKAENFRIDMNDDVIKRAMVTYNGKVNWPPEPLAVSAQAKKTEAGKPSPEEEAAK
                     KAGKVKKALISNVIWLAVVGALLFLLGKVAPADFMGHITAFVLAVFVGWNVIWNVTHA
                     LHTPLMSVTNAISGIIIIGGLLQIQNDFSDPRTIMAFIAILIAFINIVGGFTVTYRML
                     KMFKK"
     gene            complement(2835..4190)
                     /gene="gdhA"
                     /locus_tag="PK502_01845"
     CDS             complement(2835..4190)
                     /gene="gdhA"
                     /locus_tag="PK502_01845"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPG99590.1"
                     /translation="MKNIALQEKVNAFMASLTAKNPGETEFHQAVREVVESLMPFIDE
                     NPIYSDEKILERITEPERVIIFRVPWIDDRGNLQINRGYRIEMNSAIGPYKGGLRFHP
                     TVNLSILKFLAFEQVFKNSLTSLPMGGGKGGSDFDPKGKSDKEVMRFCQSFMTELYRH
                     IGPNTDVPAGDIGVGGREIGYLFGQYKRIVNEFTGVLTGKGLKWGGSLIRPEATGYGN
                     VYFAQEMLKTRGQSIEGKIVTVSGSGNVAQFTVEKAVELGGKVVTLSDSDGFIYDSEG
                     INTEKLAYVKWLKNDKRGRIKEYADKYKCEYYPAERPWSIKCDIALPSATQNEINGEE
                     AKTLVRNGCMCVSEGANMPSTPEAIEVFLNAKILFGPGKAANAGGVATSGLEMSQNAL
                     RLSWTREEVDNRLRLIMSNIHETCMKYGKADDGFINYVNGANIGGFVKVADSMIAQGA
                     V"
     gene            4469..5047
                     /locus_tag="PK502_01850"
     CDS             4469..5047
                     /locus_tag="PK502_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPG99591.1"
                     /translation="MEKSVKGTETEKNLLKAFAGESQAKNRYTFFSKVAKEEGFEQIA
                     EIFMTTALQEEQHAKQFFRFLEGGMVEITASYPAGVIGTTAENLKAAAMGEHEEWSEL
                     YPHFADVAEKEGFTRIATAFKMVAKVEVEHEKRYKRLLEKVEKDKVFERDEEIEWVCR
                     KCGYVHKGKKPPKNCPVCQHPQAYFEEKANNY"
     gene            5197..7686
                     /locus_tag="PK502_01855"
     CDS             5197..7686
                     /locus_tag="PK502_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPG99592.1"
                     /translation="MQGIYPDSFEQKVGFDRVRELVAGRCLCPIGLQHVESVQFSTDY
                     SLISTWLAQVDEMKSICLMEEGFPVDHFYDLTPSLRKIAAQGAWLDESELLKLRTSLE
                     TIRALLSFFKKFTDRYPAIWELAKNVAYYPTVVERINALLDKFGRIKDNASPQLAQIR
                     SAITSKQSSVSKLVQTILRSARDQGIIEQDASPSVREGRVVIPVAAANKRKLKGIVHD
                     ESSTGKTVFIEPVEVVELNNEIRELEYDERREVVRILVEFSDSIRPYLPELLGAYDFM
                     GMVDFIRAKALFAIEYQGAKPIIKETTGTYLRQAKHPLLQLSLKREGKEIVPLDIELK
                     SEERILLISGPNAGGKSVCLKTVGLLQYMLQCGFLPCVLENSEMGLYRKIFIDIGDEQ
                     SLENDLSTYSSHLLNMKYFLKNATPETLILIDEFGSGTEPSAGGAIAEAILQGFANTG
                     TYGVITTHYSNLKHFAASTRGIVNGAMLFDSSRIEPLFKLEIGKPGSSYAFEIARKIG
                     LPDSIINEATAKVGDDYVTFEKHLREISRDKRYWEKKRENIRLASKKAEAEEAQLTRE
                     LEELASQRKEILAAAKKNAQELLAETNRKIENTIRIIKESNAQKEETRKARQELEQFK
                     QQVDEAAHNLNDEVERKMERIKQKQEKRALHRKAKEPAASSRTEPVAPPPLTIGDRVK
                     IIGSDLLGEVVGLSGQNVSVAVGNLISMVNLERVQRISTAEYRNANKAVTQPQVGTGF
                     DTYKRRLNFKSDIDIRGARADEALETVQNLVDDALMLGFGRVRILHGKGNGILRSVIR
                     DYLKSTPGVKAFSDEQEEFGGAGITVVDLDV"
     gene            complement(7809..8732)
                     /locus_tag="PK502_01860"
     CDS             complement(7809..8732)
                     /locus_tag="PK502_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPG99593.1"
                     /translation="MKNSRKDLVRIGVFYDGNYFLHVSNYYNYFHPRKKRLNIAGLHE
                     FIRNLVASEEEADPRNCQVIEAHFFRGRISAQEASQRGNQLYNDRVFDDILMSEGVVT
                     HYLPIRPRGGRREDKGIDVWLALEAFEQTFYKRFNVLVLITSDGDYVPLIRKVNSLGT
                     KVMVLSWDFDYTDDQGREVTTRTSQELIREVTYPVAMHELINSGLKSKDRLTENLFTV
                     QDFGQAKPVEKPVSPEEIKQARVLSLNSGYGFIQYPPNNLFFYYLDVVDGDFDELIEG
                     DMVEFTISKNDRGQDVAKNVRKIPEYNANKW"
     gene            9443..9889
                     /locus_tag="PK502_01865"
     CDS             9443..9889
                     /locus_tag="PK502_01865"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HPG99594.1"
                     /translation="MRQTIPFRIVELEPDSYHMVVEASMSGKSVHLIVDTGASRSLID
                     ISSDSGLRVEGVDETLAVGFNTDKISIQLAIFPYIGVGGFTFSDFVAAVTDLDALRSL
                     YSKVAGMDIAGLLGCDFLVRYVSSINFRTKRIYLKQLTAEVKSSQE"
     gene            complement(9921..10442)
                     /locus_tag="PK502_01870"
     CDS             complement(9921..10442)
                     /locus_tag="PK502_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPG99595.1"
                     /translation="MKKLSGYFLSVLLVAAMLSACDGNNPAKGQQESAPAAPTKTGSK
                     ITGTYAVEITREEFLTKILDYTQEPTNVKYLGNKPAIVDFWASWCGPCRIASPILEEL
                     AKEYHGKILVYKVDTQKEQQISAELGIQSIPTFIFFPVDGKPFATSGIASTPEDTRKM
                     FVEIIEKELLGNR"
     gene            complement(10480..10683)
                     /locus_tag="PK502_01875"
     CDS             complement(10480..10683)
                     /locus_tag="PK502_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPG99596.1"
                     /translation="MHAVVVNQEKCPRNHPCPVVRICPTGAISQKSWNSAPEISADDC
                     LACGKCVTSCGYGAFRFDNAGAK"
     gene            complement(10811..11641)
                     /locus_tag="PK502_01880"
     CDS             complement(10811..11641)
                     /locus_tag="PK502_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99597.1"
                     /translation="MKRLNVIFAASLMMMAAISFTSCQKDDGDTTDPLVTTMQEGDEV
                     DSYFDETMSEVDELTYESDSKSQMDMAVINSMGQGTRTRKTSWEGDIRIDTVTYNNFV
                     NGNARFERVKNGQIIIRTTGHYLGETFIREVSFNNFTINGNLIEGNKRIQKTSQYTYE
                     IQLENGKVTFSDGTTHTRTMTRTRTMVEGYDTPYYIWDDVYTIEGSSTGVNRQGNAYT
                     HQITNALVVRMDCRWIVSGTFNVTVNNNSMSLDYGNGVCDNLATATINGRAYEVRLRK
                     "
     gene            complement(11690..12568)
                     /locus_tag="PK502_01885"
     CDS             complement(11690..12568)
                     /locus_tag="PK502_01885"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPG99598.1"
                     /translation="MKRFQKIPDNSFERNKTSLGNELFQSFRVPEGIGHDASWESILR
                     SIKSPDISVSPRIPNLIFKVAATLIVLIGLGSMIWLYGLGNVEVSVPYGQLATHVLPD
                     SSQVTLNADTRLTYNRNRWLLQRRVRLDGEGLFKVMKGSRFTVQTETASTSVLGTVFN
                     VFARKGVTRVSCLEGKVKVVAKESKKEAYLTNGMEAKTMGFELSKPDSKKEEFKTSSW
                     TKGEFYYTNTPIGQVFDELERQFDVDLFFDGDTTRTYTGYFKKDNLNSALRLVCIPMD
                     MKWSVNDTLVIINEIK"
     gene            12938..13819
                     /locus_tag="PK502_01890"
     CDS             12938..13819
                     /locus_tag="PK502_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99599.1"
                     /translation="MRKKYMQFTTTITGEQVENFCKSTADENPIHNQQHMRELGKEIV
                     VPGLYIFSLIVNFLQKNESLNFNTFRIFFGNIVGINEAIDLGFERSPISESQRYLFAR
                     TGADCFSLDNSRSFADIRSNGIGMEERGRHVPITYNKEQVHIFRKLLGLSNAALLSFL
                     FSVSLASPMLISTIRKPVTDVDKEIAMLFNKSVYPEGISPLYQSLEIFRLENSTILEP
                     EGQVDYWVQLEKEKAGKFYAANVSCGQSGKVMYNSRYRLVAVPNKVMLRMVRELKNQG
                     SKKVEEAKTLNSGRTLA"
     gene            complement(13785..16469)
                     /locus_tag="PK502_01895"
     CDS             complement(13785..16469)
                     /locus_tag="PK502_01895"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPG99600.1"
                     /translation="MVNRFRIAGAVVVFSLLALMAIPHALMGQALITEKFSNAPLVES
                     LNLISKKYAVKVAFDNEALSGYNINARFTNEPVEKVMSRLLKDTNFEYIVIDNVYVIR
                     RKIAVTTDTPKVNPLKFNGVVRDRNSGESLPYASIKVVNTQLGTAANSDGFFSLAQTQ
                     TDSLAKADSLYIRVSYVGYKPIEMKVARFSHTNSPLVVELDRNDLEIKEVKVTDEYSS
                     IVNHGFRPGELVWSSRQVAQVPTFSGIDAIAPLMLLPGVDGTTESLSGLTVRHSAADK
                     NLVTFDGFTLYNIDHFFGSFSSLNSKVVKDIRILRGGFDAQWGGRASSVIEITGKTGN
                     EHNLVVDAGADELSADVSVEGPIGKKLSYVVAARRSFTDYYRSPLYNSLMGVTRPDFS
                     SVQALANTSDADVVKPTFYYYDANAKVSFKPNIRDIISVSGYTSGDKLTYNQNVQNPY
                     VDENADLSTTGVGFRWARQWGSSFYHNLTIGGSSFNLNYVHQDSIMRYRQASQRDTVL
                     KNYDLENVLNDFSLNLLMQFKPDVHNTLEGGLSSNLVNLNYFESYFHNVNGLSIIDTT
                     HSSDNLSALYSGWLQNTFAGGKLKALTIGFRATYHNLSGKFYFEPRLQAIVAPTPNLS
                     LKLAAGIYNQYVNRIVQANSSGVYRNFWIVSDGKLFPVVKSQHLIAGMTWEYKGWLVD
                     VEGYLKYTEGISFIQNVTRRVNGNQFKTTSLLLTLDSRATGVDLLVRKDWSKGQVWAA
                     YSLSRSFNESPKLNGGEEYYALDDHLHELKIAGTFTVGKWHLSASWIYGSPKPWDEIL
                     LTSSLQLSPDYVKNSVRLIPYHRLDAGLAFVQRVDKVEVTAGVKAFNLYDRQNVIAKP
                     FSLSDTPVQDYLAGRALITFQEIIGMDFTPMFYLNLKF"
     gene            complement(16541..17434)
                     /locus_tag="PK502_01900"
     CDS             complement(16541..17434)
                     /locus_tag="PK502_01900"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPG99601.1"
                     /translation="MTTPKEHTKQVSETGHEELMDAIKALQVPPSTRSKEDAWNMIMQ
                     SIATTVQPKETTRINSLRVWLTTAAAAAIVVVSCFSYLWFSRVSVSSPRGVATDVILP
                     DGSTAKLNADSWLKYPKFYSLVGRKLKIEGEVFFTVTPGRSFVVSDDFKRQVEVVGTK
                     FNMATRGSTFKVACFSGSVMVSNPGSERVQLGKGEVAEAAGKTLKVSKDELHIADSPG
                     WTNGEFKFVGVPLSEVLTELERQFNIKVTAMDFNPNGRTFTGYFTKSSLEQALELVAQ
                     PMSLRYEVSSDSTVVKIYSNK"
     gene            complement(17431..17952)
                     /locus_tag="PK502_01905"
     CDS             complement(17431..17952)
                     /locus_tag="PK502_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HPG99602.1"
                     /translation="MTLPTGSYSQEELSWFRAIFDDYYESIRSFAYYKTGSVDLADDV
                     VQEVFLKLWNIRSSVRNDTVKTLLYTIATNLIKNHFKHQRVVYNFQVSKSTQDEAESA
                     DANVRMDDMNRQLQDALASIPDAAREVFLMNRIDGFTYSEIAQRLNLSVKTVEKRMSE
                     AIRLLRNKFGYKL"
     gene            complement(18227..21205)
                     /gene="infB"
                     /locus_tag="PK502_01910"
     CDS             complement(18227..21205)
                     /gene="infB"
                     /locus_tag="PK502_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPG99603.1"
                     /translation="MTNDKSIRISKLAREFNVGVSTLVEFLKKKGYHVTPDPNAKVDE
                     DIVNLLTKEFGAEVNIKKESQKVSLKSLRDAKETLSIDEIEKKRDEDEGDSDFSNDEV
                     IIKTTGFVHEKIDDFGMEKPKIDLKVKGKIDLDNLSSQPRSSKKETPVESTTSEVPVT
                     PLKQATKEVATKKESKEDEGAKPERTKVKTPKVVGKIDLDKTSKKKAKEKPEKNSNQK
                     TKEESAIVENEMQPIVEEKLEKVPSVQEVIEKPIEVEREEIYRSTVEKLAGPTIKGKI
                     DITAFETKKKQPVASSDAVKNDKKKRRKRIKKDVGRVQLPEAEQQGTPRSNPPLLVKK
                     ADLPVNNKKSNAGGSKKKRPVKQEVDEEAVQRQIKETLAKLTTKGKTKGSKYRREKRE
                     ILNARQQEENQRLELEKKILKVTEFVSVNELASMMSVPVTDVIEVCMNLGLIVSINQR
                     LDAETMTIVADEFGYKVEFVSIELQESVKDQEDDSPEMLKPRPPIVTVMGHVDHGKTS
                     LLDYIRHSNVIAGEAGGITQHIGAYGVELENGKKITFLDTPGHEAFTAMRARGARLTD
                     VAIIVVAADDAVMPQTIEAINHASAAGVPIVFAINKIDKPTADPEKIKGELANLNYLV
                     EDWGGKYQCQEISAKKGTNVDKLLEKVLLEAEMLDLKANPDRKASGTVIESALDKGKG
                     YVATVLVDSGTLHHGDIILAGIYYGHVKAMFNERGVRITEAGPSTPVQVVGLNGAPQA
                     GDYFHVMESDKEARELANKREQLVRMQGLKTKKHITLDEIGRRIAIGNFQELNIVVKG
                     DVDGSVEALSDSLIKLSTPQIQVNVIHKAVGQISESDILLAAASNAIVLGFQVRPSMG
                     ARKLAEKEEIDIRLYSIIYDAINEIRDAMEGMLSPEIKEEITGTAEVLNVFKISKVGT
                     VAGCIVREGKISRSNKVRLIRDGIVVYTGDLESLKRFKDDVKEVSAGFECGLNINNFN
                     DIKVGDIVEGFQEVEVKRKL"
     gene            complement(21266..22504)
                     /gene="nusA"
                     /locus_tag="PK502_01915"
     CDS             complement(21266..22504)
                     /gene="nusA"
                     /locus_tag="PK502_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPG99604.1"
                     /translation="MENINLVDTFAEFKELKNIDRPTMMSVLEDVFRATLIKLYGSDD
                     NFDIIINIDKGDFEIWHNREVVEDGKVEDPGRQIALSEARKIDADFEVGEEVTSEVKF
                     ADFGRRAILALRQNLTSRIMELEKNNLYNKYKDRVGDLITGEVYQVWKKEIMVLDDEG
                     NELVLPKTEQIPTDFYHKGDSIRAVVYKVEMKNNTPQIILSRTANAFLERLFELEVPE
                     IFDGLITIKKIVRIPGERAKVAVESYDDRVDPVGACVGMKGNRIHGIVRELRNESIDV
                     INYTNNTQLFIQRALSPAKITSIKFDETTKRAEVYLKPSEVSLAIGKGGYNIKLASQL
                     TGYEIDVYRESEGEDEEDVNLDEFADEIEGWIIDTLKGIGCDTAKSVIEIPFKELVKR
                     TDLEEETVKEILKILKSEFE"
     gene            complement(22529..22996)
                     /gene="rimP"
                     /locus_tag="PK502_01920"
     CDS             complement(22529..22996)
                     /gene="rimP"
                     /locus_tag="PK502_01920"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HPG99605.1"
                     /translation="MISRETIEAIVAEKLEEENLFIVDISISPKNTITIMLDGEKGVS
                     IKSCVAISRLVESKLDRNVEDYGLEVTSAGLGQPFRVVRQYSINIGREVEVLLLDGNK
                     FKGVIVEAGSDNFSIEYSEKVLMDDGKHKQVVQQRKSFPYNVVKSTKAVISFK"
     gene            23237..24364
                     /locus_tag="PK502_01925"
     CDS             23237..24364
                     /locus_tag="PK502_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPG99606.1"
                     /translation="MRIAVNTRLLIHGKLEGIGWFTYETFSRMAIDHPNDEFFFIFDR
                     PFSREFIFAKNIHPVVLGPPARHPILWYIWFEFSVNRFLKKLKPDIFISPDGYLSLKA
                     KTPQLAVIHDINFYYYPKGLPWLTSLYLNKYFPKFAGKAKRIVTVSEYSKNTLVEAYK
                     VNPQKIDVVYNGASDNFKPLTEIERAEQRNQLTDGWPYFVFVGAFSPRKNISSLINAF
                     DMFRRKTNIQAKLVLVGETMFKASSIEAAHHKSKFKDDIIFTGRKGLPELSRIVGAAQ
                     ALIFPSLFEGFGIPVLEAILCEVPVAASNSTSMPEVAGNAAIYFEPTSDESIAEAMER
                     IWMDTQLRQRLISNARVQRQKFSWEQAASRLYDIIAKTIES"
     gene            24370..24975
                     /locus_tag="PK502_01930"
     CDS             24370..24975
                     /locus_tag="PK502_01930"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPG99607.1"
                     /translation="MGCLRTIHTPGLVEAGCDEAGRGCLAGPVFAAAVILPPDFYDNS
                     IDDSKKLTLKQRLAARQLIQHYALDWAVARVSNEEIDRLNILNASILAMHMAIKALTR
                     ATPQHLLVDGNRFKNFPGIPHTCIVGGDHTYQSIAAASILAKTYRDELMNELSEQFPG
                     YGWRTNKGYPTKLHRRAIAELGPTPYHRLTFALLPQSKTKC"
     gene            25046..27196
                     /locus_tag="PK502_01935"
     CDS             25046..27196
                     /locus_tag="PK502_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPG99608.1"
                     /translation="MKKRFLALLTGLLLLTGAMVRADEGMWLLPLLKQLNIDVMQQKG
                     LKLSADDIYQINQSSLKDAVIIFGRGCTGEIVSDQGLILTNHHCGYGAIQQHSTPEHD
                     YLADGFWAKSLQEEIPTPGLTATFLVRIEDVTDQVNAALNPSMTEAERNQAIQTVGKK
                     IASSATAGTHYQASVRSFFGGNQFFLIVNEVYNDVRMVGAPPSSIGKFGADTDNWMWP
                     RHTGDFSVFRVYAGKDNQPADYSPDNVPLKPKRFLPVSIKGLQNGDFAMIMGYPGSTE
                     RYMTSVEVEEMMSISNANRIYIRGIRQDILLKDMLADKAVNIKYASKYSGSSNYWKNS
                     IGMNKSLKRLKVIDVKKAGEAEFTRWVNANPERVQMYGEALNLINEGVTGRKDYPHAT
                     QYIGEALSRGTELMSIAGIAERIVEPLKSGNNEELSKNVAMLKSRATMFYKDYNAPTD
                     RKVAKAMFKIFSENVPVQYQPDIFNTIRTKYKNDFDRFIDEMFDKSMFVDSVKLFAFL
                     SKPNLKTLQKDPAFIAAQSINAKAMELYGLMEPFNQKLQKGQRLFIAGIMEMNKGKAL
                     YPDANFTMRLTYGEIKDYYPMDAVHYDYQTTLDGVMEKEDPDNWEFVVPQRLKDLYQA
                     KDYGRYGVNGKMPIAFISTNDITGGNSGSPVMNGNGELIGLAFDGNWEAMSGDIVFEN
                     NLQRTISVDIRYVLFIIDKYAGATRLVDEMQIVE"
     gene            27537..28049
                     /locus_tag="PK502_01940"
     CDS             27537..28049
                     /locus_tag="PK502_01940"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HPG99609.1"
                     /translation="MKNSEVLISHNGPLSYEEIGFLLNKMTAILEKYNFGITIKKKVY
                     AAMVESLENVYKHQDPIPEDAGHFPKFTLSLDDKYIYISVSNPLLKTKIPMLEDRLAR
                     VNQLDKNGLKEFYKQIILNGNVSQKGGAGLGIINIAKVTENKLDFTFSEIDGQHAYFT
                     LGIKVAHYTE"
     gene            28063..28473
                     /locus_tag="PK502_01945"
     CDS             28063..28473
                     /locus_tag="PK502_01945"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPG99610.1"
                     /translation="MEPLYIEPSEFTPRVFFDPGNTVFEISGFSRPENTVDFYKPILR
                     WLDEYEENVLNKNTEYSKSNMVLNLKMTYFNSATSKYLLDLLMKFMKFHSKGNTVEIN
                     WFYDEDDDEILESGEEISEMINFPFNYIPITPTQ"
     gene            28515..28916
                     /locus_tag="PK502_01950"
     CDS             28515..28916
                     /locus_tag="PK502_01950"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPG99611.1"
                     /translation="MNSLTIEPTTISPHVSFDMDKGVFIISGYSRPENVRDFYAPIIS
                     WLELFRDQLFREQSEGKTINPIIFNFNLIYFNSSSAKFLYDIVMLLDEVQKKGINLTI
                     KWHYSKDDSELREAGEDLSDMAQVPFDFIEV"
     gene            29211..>29583
                     /locus_tag="PK502_01955"
     CDS             29211..>29583
                     /locus_tag="PK502_01955"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HPG99612.1"
                     /translation="MNTILYIRLTIDNSNPAIYRDIQVKSQTSLEVMHEIIQATFEWS
                     NLHSHYFKDSAGNIIKDKKGILIGSIITTSGSITYVYDYADSWGVRIEVLEARDIMDE
                     NFTPICTDGRRNSPPEESGGIV"
BASE COUNT         8207 a         7005 c         6244 g         8127 t
ORIGIN      
        1 accggttttt acctcctggc caaagcctcc ggaaataacc gcaaaaaacg acctgttcat
       61 ggccctgcac atggtaatgg aaaggatagc ccccgagctg cccaccagcg cccctgtaac
      121 tatcagcaaa tcgttgccca gcataaaacc cgaagcagca gcggtccatc ccgaaagcga
      181 gttgagcata gaaattacta ccggcatgtc agcacctcca attgccataa ccagcattac
      241 tccaaaaaat gctgcaataa cggtcatggc aataaggaac ggcataccat gagtgccacc
      301 ctggccaaca aacataacgc ccaagactac tgaagccaac aaaagaacta cattaaccac
      361 atcctttcct ttaaatacaa gaggtttact ggtaatattt tccgttaact tcccccaggc
      421 aataattgaa ccggtgaagg tgatggcccc gataaatatt cccacaaaaa tttccaccat
      481 gtggatagcc ctcacggcgc cagccatgct aacggttttg gggtctatga atgagctaaa
      541 acccaccaga acggcagcta gtccaacaaa actatgcaaa agggcaatca actggggcat
      601 ggccgtcatc tgcaccttgc gggcaacaat aacgccaata accgaagcaa ctaccatggc
      661 aacaataatg taaacgtagc cttgaatatt accgccgaac agggtggcac acagggcaat
      721 tgccatgccc acaatgccat aaattacacc ctttttggcc gattcctgtg acgataaccc
      781 cgacaagctt agaatgaaaa gaattgaggc gaaaaggtag gctgcaattt gaatattggt
      841 tgaaatcata ctccgtttac tttttaaaca ttttcaacat cctataggta accgtaaaac
      901 cgccaacaat gttgatgaag gcaatcagta tggctatgaa tgccataatg gttctagggt
      961 cagaaaaatc gttctgtatt tgcagcaaac cgcctatgat aatgatacca ctaatggcgt
     1021 tggtaaccga catcaacggg gtatgcaggg catgggtaac gttccatata acattccagc
     1081 caacaaaaac agccagaaca aaagcggtga tatgccccat gaaatcagca ggtgcaacct
     1141 ttcccaggag gaataataag gctccgacaa cggcaagcca aatgacgttg gaaatcagcg
     1201 cttttttaac ttttccggcc tttttggcag cttcctcctc aggggaaggc ttaccagctt
     1261 cggtcttttt ggcttgtgcg ctaaccgcca gcggctcagg aggccagttt accttgccat
     1321 tgtaagtaac cattgcccgc ttgatgacgt cgtcgttcat gtctatcctg aaattttccg
     1381 cttttcccat atcgtcgagc aggttacaca ggttgttgcc atataggaag ctggcctggg
     1441 tgggaagggt atcaaccctt ccggcaatca ccacaccgtt tggagttgtg taaacctcat
     1501 cctttttggt tagcacgcag ttaccacctg taggagccgc cagatcaata ataaccgagc
     1561 ccggcttcat agccataaca tgttcctcaa gcagcagctt tggagcggcc ttccctggta
     1621 tttgcgcagt agtgatgatg atatcaactt cagcagcttg cctcttaaac agcgccattt
     1681 cggcatcaat ataagcctgg ctcatctcct tgctatagcc cgaagcagtg gagccatcct
     1741 cgtcaatctc aaccgttatg aattgtgctc ccatggactg aatttgctct ctggcctcct
     1801 tacgcgtatc gaatgcccta acaattgctc caagcgattt tgccgtgcca atagctgcca
     1861 ggcctgcaac tccagttccg ataaccagca ctttggcagg ctcaaccttc cctgcagcgg
     1921 taatctggcc gtttaaaaac cgtccaaaat agttagcggc ttcaactaca gctctgtacc
     1981 ccgaaatgtt ggccatagac gatagcacat ccattttctg tgccctcgaa attctaggga
     2041 tggcatccat ggctatagca tttatacctg cctcggcaag cttgcccagt aggggcaaat
     2101 tttgagcggg aaacaggtag cttaatagca atgtgccacg cttcatcaaa ccaacctcgg
     2161 cttcggtagg cggttgaacc tttagtatta tatccgacga tgagtacaac tcttgagccg
     2221 aagagacaac ctcagcaccc gccgctacat actcctcgtc caagaacttg gcccggacac
     2281 cacatccgga ctctactaac acctcaaaac cctgcttttt caatctaacc acggttttgg
     2341 gagtagcggc aacccttagc tcatgttgaa gtaattcttt aggaattcct acattcatat
     2401 cttgtaaaat ttaatgtcgg tctctacaaa catcatctat ttactgtatt ttaaaagaat
     2461 ttaacgggac ataatgttcg cattaagctt catgtttcat attactcccg ttaacacatt
     2521 gcttttgcaa cctgccaccc atcatttatc tgttttccta accattagca accgtaggaa
     2581 gatcaactaa atgatctgct caacttaact atctccataa ataaagaata attacaagtt
     2641 ggcacttcgg gtttaaaaat ttgatgagtg catttttctg ccaaatgtaa gtggtaattt
     2701 tgaaaaggaa tatgacataa gctagcaagt tgcatcaaaa tggtaaagaa agattaaccc
     2761 cactctcctg aatatcaagt ttaggcacgc tatcgccaaa agatgcgatt ttaaccaaat
     2821 gggtgaaaaa ctttctacac agcaccttgg gctatcatag aatcggccac cttcacaaat
     2881 ccgccaatat tggcgccatt cacatagtta ataaaaccat catctgcttt accgtatttc
     2941 atacaggtct cgtgaatgtt ggacatgata aggcggaggc ggttgtcaac ctcttcacgg
     3001 gtccatgaaa ggcgaagtgc attttgtgac atttcgagtc ctgaagtagc tactccacca
     3061 gcattggcag ctttgccagg gccaaaaaga attttggcat tcaggaaaac ctctatagcc
     3121 tcgggtgtcg aaggcatatt tgcaccctcc gaaacacaca tacagccatt tcgaaccagc
     3181 gtttttgcct cttccccatt aatttcgttc tgggttgcgg agggtagtgc aatgtcgcac
     3241 tttatactcc atggacgttc ggcggggtag tactcgcatt tatacttgtc agcatactcc
     3301 ttgatacgcc ccctcttatc gtttttaagc cattttacat acgccaactt ttcagtgtta
     3361 atgccttctg aatcatagat gaatccatcg gaatcggaaa gggttaccac tttccctcct
     3421 agttcaactg ccttctcaac tgtaaattga gccacattgc ccgaacctga aaccgtaaca
     3481 attttaccct caatgctttg gccgcgagtt ttcagcatct cctgtgcaaa atacacgttg
     3541 ccataaccgg tcgcttcggg gcgaattaac gaccctcccc acttcaaccc cttgcctgta
     3601 agcacacccg taaactcgtt tactatacgt ttgtactgcc caaacaggta tccaatctcc
     3661 cggcccccaa ctcctatatc tccggcagga acatcggtat tgggcccaat atgccggtaa
     3721 agttccgtca taaagctttg gcaaaagcgc ataacctcct tatcgctttt tcctttggga
     3781 tcgaagtcag atcccccctt ccctcctccc atgggtagtg aggtgaggct attcttaaac
     3841 acctgctcga atgccagaaa tttaagaata ctcaggttga ctgttgggtg aaagcgtaat
     3901 ccccccttgt atgggccaat ggcactgttc atctctattc tgtagcctcg gtttatctga
     3961 aggttacccc tgtcgtctat ccagggtaca cgaaaaataa tcaccctttc gggttcggta
     4021 atgcgttcca agatcttttc gtccgagtat atcggattct cgtctataaa aggcattaag
     4081 ctctcaacga cttccctaac ggcctgatga aattccgttt cgccggggtt ttttgcagta
     4141 aggcttgcca taaaagcgtt caccttttcc tgaagtgcaa tgtttttcat agtcaatagg
     4201 tttaaattgg ttaggttgtt tttgagcaat ttaatgtaaa aaaaagccca acgggatatt
     4261 ttttattgta tttttttgat attttttaaa ttaataatca actcacacaa ttatacttaa
     4321 gccataggtt ttatggaatc aataatatta ttggaagtct tgtagcatca aggtagagtt
     4381 ttccaacaag ttaaagtttt acataaaaga ctgttaacta tttttgctac cttagctggg
     4441 agtataacaa aactaaaagt caagcaaaat ggaaaagagc gttaagggaa ccgaaaccga
     4501 aaaaaatctc ctgaaggcct ttgcaggaga atcgcaggca aaaaaccgtt acaccttttt
     4561 ctcaaaggtt gccaaggagg aaggctttga gcagatagcc gaaattttta tgactacagc
     4621 cctacaagag gagcagcacg ccaagcaatt cttcagattc cttgaggggg gcatggttga
     4681 gattaccgcc tcataccctg ctggtgttat tggaacaacc gccgaaaacc ttaaggcagc
     4741 agccatgggt gaacatgagg aatggtcgga gctctacccc cattttgcag atgttgccga
     4801 aaaggaaggt tttacgagaa ttgccacagc cttcaaaatg gttgcaaagg tagaagtgga
     4861 acacgaaaag cgctacaaaa ggcttctcga aaaggttgaa aaagacaaag tttttgaacg
     4921 cgacgaagaa attgagtggg tgtgccggaa gtgcgggtac gttcataagg ggaaaaagcc
     4981 acccaagaac tgcccggtgt gtcagcatcc tcaggcatac ttcgaggaaa aggcaaacaa
     5041 ctactaagca gtccttgcat cttcatggcc ggtttaccca ccggccattc tttttccatt
     5101 gaccttaagc ctgcaacatt tttgtaactt tgcagcaggt tacagttggt tttttaatcc
     5161 ttgtttttta cctctacata aaaggaaaat aatgtaatgc agggcattta ccccgacagc
     5221 tttgagcaaa aagtgggatt tgacagggta agggagctgg tggctgggcg ctgcctctgc
     5281 ccaattggtt tgcaacatgt tgaatctgtt cagttttcaa cagactacag cctgatatca
     5341 acctggctgg cgcaggttga tgagatgaaa tccatctgcc tgatggagga aggcttccct
     5401 gtggatcatt tctacgattt aaccccatcg ctaaggaaaa tagcggctca gggcgcatgg
     5461 ctcgatgagt cggaattact aaagcttcgc acctcgctgg aaaccattcg ggcgcttctt
     5521 agcttcttta aaaaatttac cgacaggtat cccgccatct gggagctggc caaaaacgta
     5581 gcctattacc ccaccgtagt ggagcgcata aacgccctac tcgacaagtt tggaagaatt
     5641 aaagacaacg cttctccaca gctggcccaa attcgctcgg ctataacatc aaaacaatca
     5701 tccgtttcaa agctggtgca aaccattctg agatcagctc gcgaccaggg cattattgaa
     5761 caggacgcct caccctcggt tagggagggc agggtggtta ttccggttgc agcggctaat
     5821 aagcgtaagc ttaagggaat agttcacgac gaatcatcaa caggcaaaac ggtttttatt
     5881 gaaccggttg aggtggtaga gctgaacaac gaaataaggg agctggagta cgatgaacgc
     5941 cgcgaggttg ttcgaatact ggttgagttc tccgatagta ttcgccccta tcttcccgag
     6001 ctgcttggag catacgattt tatgggaatg gttgatttta ttagagctaa agccctattt
     6061 gccattgagt atcagggtgc aaagcccata atcaaggaga ccaccggcac ctacctccga
     6121 caagccaagc acccactact gcagctgagc ttgaaacgcg aaggaaagga aatcgtcccg
     6181 ctggatattg aacttaaatc cgaagagaga atacttctga tttccggccc aaatgcgggc
     6241 ggtaaatcag tatgcctcaa aaccgttggc ctactccagt acatgctgca atgcggtttt
     6301 ctgccctgcg tattggaaaa ttcagaaatg ggcttatacc gcaaaatctt catagatatt
     6361 ggcgatgagc aatcgctgga gaacgacctc agcacctaca gctcccacct gctaaacatg
     6421 aagtatttcc tgaaaaatgc cacacctgaa acactcattc tcatcgatga gtttgggtcg
     6481 ggcaccgagc cgtcggcagg aggagcaata gccgaggcaa tccttcaggg ctttgccaat
     6541 accggaacct acggtgtaat taccacccac tactccaacc tgaagcattt tgccgccagc
     6601 actcgtggaa ttgtaaatgg cgccatgctt tttgacagct cccgtataga acccctattt
     6661 aagctggaaa ttggcaaacc gggtagcagc tacgcatttg aaattgcacg aaagattggc
     6721 ctaccggaca gcattataaa cgaagctacg gcaaaggtag gcgatgacta tgttaccttt
     6781 gaaaaacatt tacgtgaaat ctcacgcgat aagcggtact gggagaaaaa aagggaaaac
     6841 attcgtttgg ccagcaaaaa agcggaagca gaagaagcac agctgaccag ggaactcgaa
     6901 gagctagcca gccagcgtaa ggagattctg gctgctgcaa aaaaaaatgc acaggagctg
     6961 ctagctgaga ccaataggaa aattgaaaac accataagga ttattaaaga atcgaatgca
     7021 caaaaggagg aaacccgcaa agcccgtcag gagctagagc agtttaagca gcaggttgac
     7081 gaagctgccc ataaccttaa cgacgaggta gagcgaaaga tggagcgcat taaacaaaaa
     7141 caagaaaaaa gggcgttgca tagaaaagca aaagaaccgg cagcaagcag cagaacagaa
     7201 cctgtagcac cccctccatt gacaattggc gatagggtaa aaattatcgg aagcgaccta
     7261 cttggagaag tggtaggcct gtcgggtcaa aatgtatcgg ttgctgttgg taaccttatc
     7321 agcatggtaa acctggaaag ggtgcaaagg atatccacag cggagtatag gaatgccaac
     7381 aaggctgtaa cccagcctca ggttggaacg ggtttcgata cctacaagcg ccgcctcaac
     7441 ttcaaatccg atattgacat ccgtggagca cgagctgatg aggctcttga aacagtacaa
     7501 aatctagttg acgatgcctt aatgcttggc ttcggaaggg taagaatact tcatgggaaa
     7561 ggtaacggca ttctccgcag tgtgattcgc gattacctta aatctacacc gggtgttaag
     7621 gctttctccg atgaacagga ggagtttggc ggagcaggaa ttaccgttgt tgatttggat
     7681 gtgtaggtct ggcatacata cggtagtttt ttccctccat aaaattctca ggaaaacaat
     7741 ttcagcttaa aagggttgag cgaattctcg cccaacccgt tacgctagcc attttaattt
     7801 gccaccaact accatttatt ggcgttgtac tcaggaattt tgcgaacatt cttagccaca
     7861 tcctggcccc tgtcgttctt agagatggtg aactccacca tatccccctc aattaactcg
     7921 tcgaaatcgc cgtccaccac atcaaggtag tagaagaaaa ggttgttggg aggatactgg
     7981 ataaacccgt agccgctatt caggctaagg actctggctt gtttgatctc ttcggggcta
     8041 acgggctttt ccactggttt agcttgacca aagtcctgaa cagtgaagag gttttcggtt
     8101 aacctgtcct tacttttcaa ccccgagttt atcagctcgt gcatggccac cgggtaggta
     8161 acctcgcgaa taagctcctg cgaggtccgg gtggtaacct cgcgaccctg gtcatcggtg
     8221 tagtcaaaat cccaagagag caccatcacc ttagttccca aagagtttac cttacgaatc
     8281 aggggaacat aatcgccatc ggaggtgata agcaccaaaa cgttaaaacg cttgtagaag
     8341 gtttgctcaa aggcctcgag tgccaaccaa acatctatac ccttatcctc acggcgtcca
     8401 ccccgtggcc taattggcag gtagtgggtt acaacgccct cggacatcaa aatatcgtcg
     8461 aaaacccggt cgttataaag ctggttaccc cgttggcttg cctcctgggc gcttatcctg
     8521 cccctaaaaa aatgggcctc tatcacctgg cagttccgag gatctgcttc ctcctccgaa
     8581 gccaccaaat tccttataaa ctcgtgcaac ccggcaatgt tcagcctctt ttttcttgga
     8641 tgaaagtagt tgtagtagtt gcttacatgc aaaaagtagt ttccatcgta aaaaactcct
     8701 attcgcacaa ggtcttttct tgaatttttc ataagaataa aatttagtta tatgcttgat
     8761 tagtagttat ttggttttga ttactctgta atactcccgc cctgttggca caactcccat
     8821 ttactctgca caacgtcaga gactatggtt gctccactag aaagtcctat acttccaacg
     8881 ctaatttatc gcttagcttt acaaaatgca tcaaggcaga taccggtcgg catgaagatt
     8941 taaaactctt tgcgcaattt tactcttgat actaagcggt tagctcattc ttttaatatt
     9001 gcatgctctg gtatatcgct aaagagttta ttttttcaac ctagacaagc ttaccagcaa
     9061 ttgcatacgg ctcaagctat aaaatcataa agcgccatgt gagtatttca acttaactta
     9121 ttttttaaat gcaaaatgac ctatcaacct gcctgtctat ttgattgtta aatcgtaaaa
     9181 ccaatatgaa tgtttatatt aaaatttacg tggtttaaac atatcttttg gataggcaaa
     9241 aatacggttt tcaggcgtaa ttacaagaac atcataggga gtaattggtt caaaattgat
     9301 agaaattagc taaatttttg atttcaaaat ctcggggagc aaaagcagac cattacgaga
     9361 aaagccaaat gataaaaaga ttgctagctt tgcagcagaa tagagaagtt aaccaggagt
     9421 tattcttcat acaagctatt gaatgcgcca aacaatcccc tttcgtatag tggagctgga
     9481 gcccgacagc tatcatatgg tggtggaagc ctccatgagt ggtaaatcgg tgcacctcat
     9541 cgttgacacc ggagcatcac gctcgcttat agacatatcc tctgatagcg gcttgcgggt
     9601 tgagggagtt gatgaaacgc tggctgttgg tttcaatacg gataaaatca gcatccaact
     9661 tgcaattttt ccatacatcg gagtaggagg ctttactttt agtgattttg tggctgcggt
     9721 taccgacctt gacgccctgc gaagcctcta ctcaaaagtt gcaggaatgg atattgcagg
     9781 tttgctgggg tgcgactttt tggttcgtta cgtctcgtct attaacttcc gaacaaagcg
     9841 aatttacctc aagcagctga ctgctgaggt aaaaagtagc caagagtaag taattcccaa
     9901 tttaagctat gcttatgggg ctagcggttt cctaggagtt ccttttcaat aatttcgacg
     9961 aacattttgc gggtatcctc gggggtgctg gcaattccag aagtagcaaa gggcttgccg
    10021 tccactggga agaaaatgaa tgtggggatg ctctggatgc ccagctcggc tgaaatttgc
    10081 tgctccttct gggtatcaac cttgtaaacc agaattttac cgtgatactc ctttgcgagc
    10141 tcctcgagta tgggggatgc tatgcggcat ggcccgcacc agctagccca aaaatcaaca
    10201 atggcaggct tattgccaag atatttgaca tttgtaggct cctgagtata atccagaatt
    10261 ttagtcagga attcctccct ggtaatctca accgcgtagg taccggtaat ttttgaaccc
    10321 gttttggtag gcgctgctgg agctgactcc tgctgaccct ttgcagggtt gttcccgtcg
    10381 catgcgctca gcatggcagc aacgagtaga actgacaaga agtatcctga tagttttttc
    10441 atttgagtaa tcaatttatg tagttgaact gtaaagtagc tatttcgcac ccgcattatc
    10501 aaacctgaag gcaccatacc cacaactcgt tacgcacttg ccacaggcca ggcaatcatc
    10561 agcgctaatt tcaggtgccg agttccaact cttctggctg attgcccccg ttgggcatat
    10621 ccttactacc gggcaggggt gatttcttgg gcacttctcc tgattaacaa ctacagcatg
    10681 cattttgttt gaattttaat ggttaacatt gcaaatttaa tacatgtatg tatatattta
    10741 catatttatt catatatttt aacaaaaaaa ggaggctact gcctcccttt aggtttacac
    10801 attgtttgga ttactttctc agcctaacct cgtaggccct tccgttaatg gttgcagtag
    10861 ccaggttatc gcaaactcca tttccatagt cgagtgacat gctattgtta ttaacggtta
    10921 cattgaaagt acccgaaaca atccaacggc aatccatcct aaccaccagg gcattggtta
    10981 tttggtgtgt gtatgcatta ccctgcctgt taactccggt tgagctaccc tcaatggtgt
    11041 aaacatcatc ccaaatgtag tagggagtat catagccttc taccatagtt ctggtgcggg
    11101 tcatggttct tgtatgggta gtcccgtcgg aaaaagttac cttcccgttt tcaagctgta
    11161 tctcgtaggt gtactgggaa gttttttgaa ttcgcttgtt cccttcgatg aggttgccat
    11221 taatggtgaa gttgttgaac gacacctcac ggataaaggt ttcgccaaga taatggcctg
    11281 tggtgcgaat tataatctgc ccattcttca cgcgctcaaa cctggcgttg ccattgacaa
    11341 agttattata ggtaaccgtg tcaatcctaa tatcaccctc ccacgaagtt ttcctggtgc
    11401 gggttccttg tcccatgctg ttaatcacag ccatatccat ttgcgatttg gagtcactct
    11461 cgtaggttaa ttcatccacc tcggacatgg tttcgtcgaa gtagctgtcc acctcgtcgc
    11521 cttcctgcat ggtagttaca agtgggtcgg ttgtatcccc atcatccttc tgacaggaag
    11581 taaagctgat tgcggccatc atcatgaggc ttgctgcaaa aatcacattt aatcgtttca
    11641 tgacattaaa atttaagtta aacattgggt tttttgttat gattagtagc tacttaattt
    11701 cattaattat aactaaagta tcgttaacgc tccacttcat atccattggg atacaaacca
    11761 gcctaagagc gctgttcagg ttgtcctttt taaagtatcc ggtgtaggtt ctggtggtat
    11821 caccatcgaa aaacaagtcc acgtcaaatt gccgttcaag ctcgtcaaac acctgcccta
    11881 ttggggtatt ggtgtagtaa aattcacctt tagtccatga tgaggttttg aattcctcct
    11941 ttttggagtc aggtttactc agctcaaagc ccatagtttt agcctccatt ccattagtaa
    12001 gatacgcttc ctttttgctt tcctttgcca ccactttaac tttaccttcc aggcagctaa
    12061 cccgggtaac gcccttacgg gcaaatacgt taaaaaccgt accaagcaca gaggtagaag
    12121 cggtttcggt ttgaacggtg aacctgcttc ccttcattac tttaaacagg ccttctccat
    12181 caagtctaac cctacgttga agcaaccatc tatttcggtt ataggttaac ctggtatctg
    12241 cattaagcgt tacctggcta ctgtcgggta gtacatgggt ggccagctgg ccgtagggaa
    12301 ccgacacctc aacattaccc aatccgtaaa gccaaatcat cgaacccaaa ccaatcaaca
    12361 caattagagt ggctgctacc ttgaatatca ggttgggtat gcgtggtgaa acagaaatgt
    12421 cgggtgattt gatgcttctt agaattgact cccaagaagc atcgtgcccg attccctctg
    12481 gaacgcgaaa cgactggaaa agctcattcc cgagagaagt tttgttcctc tcaaagctat
    12541 tatccggtat tttttgaaat cgtttcattt cccgatctgc ttttgccatt aatacaacca
    12601 gaaaagattt taccctacca ctttttaaat aaatattgcc tcaagctttt catttcagag
    12661 tacgatttca gattatgaga cgctaaatat catcgagcaa gggggtagtc attttactag
    12721 ccagtaaaat attccgctca aatgcagcct ggttcttttt agtaaacttg gagaatgttg
    12781 aaaacggaat tttcctgtca tcttcaaatc ggagtaatag ataaacacga taaactacct
    12841 ggctcggatg ttacccgaaa ggcaatatcg ttacaatgtt ggctccttag ttgaaaaaag
    12901 ttaaaacgtt tgtttaactt gtaaaactta ggtagttttg agaaaaaaat atatgcaatt
    12961 cactactact ataaccgggg agcaggttga aaacttttgt aagtcaactg ccgacgaaaa
    13021 tcccattcac aaccagcagc acatgcgaga actcggcaag gaaatagttg ttccaggctt
    13081 atatattttc tccctgatag tcaactttct acaaaagaat gagtccttga attttaatac
    13141 atttcgaatt ttcttcggca acatagttgg aattaacgaa gctatcgatc ttggttttga
    13201 gcgttcgccc ataagcgaat cgcagaggta tctgtttgcc agaaccggtg ctgattgttt
    13261 ttcgctcgac aacagcaggt cgtttgccga tattcgttca aatggaatag gtatggagga
    13321 gcggggccgg cacgttccca ttacctataa caaggagcaa gtgcatatat ttcgaaagct
    13381 attagggctt tcaaatgccg ctctactaag tttccttttc tctgtatcgt tggccagccc
    13441 catgctgata agcacgataa gaaaaccggt aacagatgtt gacaaggaaa tagccatgtt
    13501 gttcaacaag tcggtgtacc cagagggaat ttcgccgcta taccaatcac tcgagatatt
    13561 taggctggaa aattcaacca ttctggaacc tgaaggtcaa gttgactact gggttcagct
    13621 tgaaaaggaa aaggccggaa agttttatgc ggccaacgtt agctgtggac aaagcggcaa
    13681 ggtgatgtac aactcccgat acaggctggt agcggttccc aacaaggtga tgctaaggat
    13741 ggtcagagag ctgaaaaatc agggtagtaa aaaggttgaa gaagctaaaa ctttaaattc
    13801 aggtagaaca ttggcgtaaa atccattcca ataatttcct gaaacgtaat taatgccctt
    13861 cctgccaggt aatcctgaac gggggtatcg gacagggaga agggtttagc tattacgttt
    13921 tgcctatcgt agaggttgaa agcctttaca ccagctgtta cctcaacttt gtcaactcgt
    13981 tgaacaaagg caagcccggc atccagccta tggtagggta ttagcctaac agaattcttc
    14041 acatagtcgg gcgaaagctg caggctggat gttagaagaa tttcgtccca gggttttgga
    14101 gatccataaa tccagctggc tgacaggtgc cacttgccga cagtaaatgt tccggcaatt
    14161 ttaagttcat gcaggtgatc atccagagca taatactcct ctccgccgtt cagttttgga
    14221 gattcattga aggaacggct gagcgagtaa gctgcccaca cctgcccctt actccaatcc
    14281 ttcctgacca gcaaatcaac tcccgttgcc ctactgtcga gtgtaagtag caaggaggtt
    14341 gttttaaact ggttcccatt cacccgccgg gtaacatttt gtataaaaga aatgccctcg
    14401 gtgtatttca ggtatccttc aacatctacc agccatcctt tgtactccca tgtcatacca
    14461 gcaatcaaat gttgcgattt caccacagga aacaatttac catccgaaac tatccagaaa
    14521 ttccggtaaa cgcccgatga gttggcttgc acaattctat ttacatactg attgtaaatg
    14581 cctgcggcca acttaaggct taaattaggt gtgggtgcaa ctatggcctg cagtctgggt
    14641 tcaaagtaga atttacccga gaggttgtgg taagtggccc taaaacctat ggttaaagct
    14701 tttagcttgc cgccagcaaa ggtgttctga agccaaccgg agtataacgc cgagaggtta
    14761 tcagaactat gggtggtatc gatgatgctt aacccattca cattgtggaa atacgattca
    14821 aagtagttca ggttgaccag gttagaactc aacccgccct caagggtatt atgcacgtcg
    14881 ggtttaaact gcatgagcag gtttaaggaa aaatcgttta gaacattctc caggtcatag
    14941 tttttcaata cggtatcgcg ctgggaagcc tgcctatacc tcataatgct atcctgatgc
    15001 acgtaattca ggttaaatga cgatcctcca attgtaaggt tgtggtagaa cgagcttccc
    15061 cactgcctgg cccaacgaaa cccaacacct gttgtgctca aatcggcgtt ttcgtcaacg
    15121 tatggatttt gcacattctg gttataggtt agcttatcgc cagatgtata accgcttacc
    15181 gaaataatgt cccgaatgtt aggcttaaag gaaactttag cgttggcatc atagtagtaa
    15241 aatgtgggct ttaccacatc tgcatcagaa gtgttggcca gagcctgaac tgaagaaaaa
    15301 tcgggacgag taacccccat cagggagtta tagagcggcg acctgtagta gtcggtaaac
    15361 gaacgtctgg cggctactac atagcttaac tttttcccta taggtccctc cacggaaaca
    15421 tcagcagaca actcatcggc gcctgcatca accaccaggt tatgctcatt tcccgttttg
    15481 ccggttattt caattaccga agaagcccta ccgccccact gcgcatcaaa tcctcctctt
    15541 aaaatacgga tatcctttac taccttgctg ttaaggctgg agaatgatcc aaaaaagtgg
    15601 tctatgttgt agagtgtaaa gccgtcgaat gttaccaggt ttttatcggc agcagaatgc
    15661 cttacggtta agccagacag ggattcggtg gttccgtcaa ctccgggtaa aagcattaaa
    15721 ggtgcaatgg catcaattcc agaaaaggtt ggcacctggg ctacctgtct cgaactccac
    15781 accagttcac ccggacgaaa accatggtta actatagacg agtactcgtc ggttaccttc
    15841 acctctttaa tttcaaggtc attcctatcg agttccacca ccaggggtga attagtgtgg
    15901 ctaaacctgg ctactttcat ttctattggc ttatagccaa catagctcac cctgatatac
    15961 agggaatcag ctttagccag cgaatcggtt tgtgtttgcg ccagcgaaaa aaaaccatcg
    16021 gaattggcgg ccgttcccag ctgggtgttg acaaccttta tggaagcgta gggtagggac
    16081 tcaccactgt ttcgatcgcg aaccacacca ttaaatttca ggggatttac ttttggagta
    16141 tcggtggtaa cggctatttt tctccgaatg acgtaaacat tgtcaattac gatgtactca
    16201 aaattagtgt cctttagcaa gcgggacatc actttctcaa caggctcatt agtaaacctt
    16261 gcgtttatgt tatagcccga taaagcctcg ttgtcaaagg caacttttac cgcgtacttc
    16321 ttgctaatca ggttgagtga ttctaccaag ggcgcattac tgaatttctc ggtaataagc
    16381 gcttgcccca taagcgcatg gggaatggcc attaatgcca gaagtgaaaa cactacaaca
    16441 gcacctgcta ttcgaaaccg gttaaccata aatttaacct tagcctaacg aaaaagttca
    16501 ccctgtgaat aatacaaccg aatgaataaa aaccctacgg ctatttgttg ctataaatct
    16561 taacaacggt tgaatcagag gaaacctcgt agcgaaggga cattggttgg gctaccagct
    16621 caagggcttg ttcaagactc gatttagtaa agtagcctgt aaaagttcta ccattaggat
    16681 taaaatccat tgcggtaact tttatgttga actggcgttc aagttcagta agcacttctg
    16741 aaagcgggac acccacaaat ttaaactctc catttgtcca accagggctg tcagctatat
    16801 gaagctcatc cttgctaacc ttcagagttt taccagcagc ctcagccacc tcgccctttc
    16861 ccagctgtac cctttccgaa cccggattac tcaccatgac gcttcctgag aaacatgcca
    16921 ccttaaaagt ggagcccctg gttgccatgt tgaacttcgt gccaaccacc tcaacctgcc
    16981 ttttaaaatc atcactcaca acaaaacttc tacctggtgt aacggtaaag aaaacctcgc
    17041 cttcaatttt cagctttcta cccaccaggc tataaaactt tgggtacttc aaccatgaat
    17101 cagcattaag cttagctgtg gaaccgtcgg gtagaatcac atcggtagct actcctctgg
    17161 gagagctaac cgaaaccctt gagaaccata ggtagctaaa acaggaaaca accactatcg
    17221 cagctgcagc tgcggttgta agccataccc tcaggctatt aatccttgtg gtttccttgg
    17281 gttgcacagt cgtagctatt gactgcataa tcatattcca tgcgtcctct ttcgaccgag
    17341 tggacggggg tacctgcaaa gctttaatgg catccatcaa ctcttcatgc cctgtttcgc
    17401 tcacctgctt agtatgttct tttggagtgg tcatagctta tagccaaatt tattcctaag
    17461 caaacgtatt gcttctgaca tccttttctc aacggttttc acgcttaaat taaggcgctg
    17521 ggcaatttca ctgtaagtaa acccgtcaat gcggttcatc aggaatacct ccctggcggc
    17581 atcgggaatg gaagccaaag cgtcttgaag ctgacggttc atatcgtcca tccttacgtt
    17641 ggcatctgca gattcagcct cgtcctgagt ggatttacta acctgaaagt tatacactac
    17701 ccgctggtgc ttgaagtggt ttttaatcag gtttgtggca atggtgtaaa gcaatgtttt
    17761 cacggtatcg ttgcgaacgg aggaacggat gttccagagt ttaaggaaaa cttcttgcac
    17821 cacatcatca gccaggtcaa cgctcccagt tttatagtag gcaaaactac ggatactttc
    17881 atagtagtcg tcgaaaatag cccgaaacca gcttagctcc tcctgtgaat agctacccgt
    17941 tggtaaagtc attagcgatt agttaacgaa acaaatgtaa ttgatgattt tcaattaaac
    18001 atagcctaat aataattgtt ttaatggcac gctgaaggaa cggaacaaac tatctcacaa
    18061 agtattcaac ctgcgttaat cagagcacta ttgggttaat cggtgtgcta ttgcacctct
    18121 tttagtacta cttaaaaaat ttacagcttc cataaaactc ccaaaacatc aaaataaaaa
    18181 aaagggagac cccaaaatag ttatctccct aaatacgtga gtattgctac aactttcttt
    18241 tcacctcaac ttcctggaaa ccttccacta tatcacccac cttaatatcg ttgaagttgt
    18301 tgatgttcaa cccgcattca aaacctgccg aaacctcttt gacatcgtcc ttaaagcgtt
    18361 taagagactc taaatcaccg gtataaacca caattccatc acgaattaac cttaccttgt
    18421 tgctacggct aattttcccc tcccgaacta tacagccggc cacggttccc accttggata
    18481 tcttaaaaac attaagcacc tcggcggtgc cggtaatttc ctccttaatt tcgggcgaca
    18541 gcatgccctc catggcatcc ctgatttcat taatggcatc gtagataatg gagtaaaggc
    18601 gaatgtcaat ttcttccttt tcggcaagtt ttcttgcccc catcgaagga cgaacctgga
    18661 atccaagtac aatggcgttg gatgctgcag caagcaggat atccgattcg gaaatttggc
    18721 ctacagcctt gtggatgacg ttgacctgaa tttgaggggt tgaaagtttg ataagggaat
    18781 cggagagggc ctcgaccgaa ccgtccacgt cgcccttcac cacgatgttc agctcctgga
    18841 agttgccaat ggcaatacgg cggccaattt catcaagggt tatatgcttc ttggtcttta
    18901 gcccctgcat acgcaccagc tgctcgcgct tgttggcaag ctcccttgct tccttgtcgg
    18961 actccataac gtggaagtag tcgcctgcct gaggagcacc gttaagtcca accacctgaa
    19021 cgggtgttga tgggccggcc tcggttattc taactcctcg ttcgttgaac atggctttaa
    19081 catggccgta gtatattccg gcaaggatta tgtctccatg gtgcagggtt cctgaatcca
    19141 ccagcacggt agctacatat ccctttccct tgtccaatgc agactcaatc accgttcccg
    19201 aggcctttct gtcagggtta gcttttagat cgagcatttc ggcctcgagc agaactttct
    19261 ccaacagctt gtcaacattg gtgcccttct tggccgaaat ttcctgacac tggtatttgc
    19321 cgccccagtc ctcaaccagg tagttaaggt tggccagctc tccctttatc ttttcggggt
    19381 cggcggtggg cttatcaatt ttatttatgg caaaaactat tggaacccca gcagcgctgg
    19441 catgattgat agcctcaatg gtttggggca taacggcatc gtcggcagct acaactataa
    19501 tggccacatc agtgagcctg gcaccgcggg cacgcatggc tgtaaatgcc tcatgaccgg
    19561 gagtgtccaa aaaggtgatt tttttgccgt tctccagctc aactccgtag gcaccaatat
    19621 gttgggttat tccacctgcc tcgccggcaa ttacatttga atgacggata tagtcgagca
    19681 gggaggtttt cccatggtca acatgcccca taacggtaac gatgggcggg cggggtttaa
    19741 gcatttcggg agaatcatcc tcctggtctt ttacagattc ctgaagttca atgctgacaa
    19801 actccacctt atatccaaat tcatcggcaa cgatagtcat ggtttcggca tcaagcctct
    19861 ggttgatgga aacaataagg cctagattca tgcacacttc aattacatcg gtaaccggta
    19921 cactcatcat ggaggctagt tcgttgaccg acacgaactc ggtaactttc agaattttct
    19981 tctccagctc aagccgctga ttttcctctt gctgacgggc gttcagaatt tcgcgttttt
    20041 ccctgcgata ctttgaaccc ttggttttac ctttggtagt aagtttggca agggtttcct
    20101 ttatttgacg ctgaacggct tcctcgtcaa cctcctgttt taccgggcgt ttcttcttac
    20161 taccaccagc attactcttc ttattgttaa caggcaaatc ggctttcttc actagtaatg
    20221 gggggttact tcttggggtt ccctgctgct ccgcttcggg aagctgaacc ctcccaacat
    20281 ctttctttat tcgttttcgg cgttttttct tatcgttttt aaccgcatca gaagatgcaa
    20341 caggctgctt tttcttggtt tcaaaggcgg taatatcaat tttccccttt attgtaggac
    20401 cggccaactt ttcaaccgtt gatcggtata tttcttccct ctcaacttct atgggtttct
    20461 caatcacctc ttgaaccgaa gggacttttt ccaacttctc ctcaacgatg ggttgcatct
    20521 cattttcaac tatagctgat tcttctttgg ttttctggtt actatttttt tcaggctttt
    20581 ctttagcctt ctttttgctg gttttgtcca ggtcaatttt tcctaccacc ttgggtgttt
    20641 taaccttagt cctttcaggc ttggcaccct catcttcctt actctctttt tttgtggcca
    20701 cctctttggt tgcttgttta aggggagtaa cggggacttc gcttgttgtt gactcaacgg
    20761 gggtttcctt tttagaactc ctgggctggg aggataggtt atccaaatca attttcccct
    20821 taacttttaa atcaattttc ggtttctcca ttccgaaatc atcaattttc tcatgaacga
    20881 aaccggtagt tttgataatt acctcatcgt tggaaaagtc gctatcccct tcatcctcat
    20941 ccctcttttt ttctatttcg tctatagata aggtttcttt tgcatcgcga agcgatttga
    21001 ggctcacctt ctgggattcc tttttaatat taacttcagc gccaaactcc ttggtgagca
    21061 ggttaacaat atcctcatca accttagcgt ttggatcggg ggtaacgtgg tagccctttt
    21121 tcttgagaaa ttccacaagt gtagaaaccc ccacattaaa ttcccttgcc agcttactta
    21181 ttcgtattga tttatcgttc gtcatacagg tggttcccgt tgtttatgtt agaaatgctc
    21241 gtgtaaagct acatgaaaga caatactatt caaattcgga ttttaatatt ttaagaattt
    21301 ccttgaccgt ttcctcttcc aaatcggttc gtttcaccag ctccttaaag ggaatttcaa
    21361 tcacgctctt ggcggtgtcg cagccaatgc cctttagggt gtcaataatc cacccctcaa
    21421 tttcatcagc aaactcatcg agattcacat cttcctcgtc ctctccttcc gattcgcggt
    21481 aaacatcaat ttcatagccg gtgagctggc tggcgagctt aatattgtac ccgcccttac
    21541 caatcgccag ggaaacctcg cttggtttaa ggtaaacctc ggctcgtttg gttgtttcgt
    21601 caaacttaat cgaggtgatt ttagcggggc ttagggcgcg ctgaatgaac agctgggtat
    21661 tgttggtgta gttgataacg tcgatgctct cgttgcgaag ttcccgaact ataccgtgta
    21721 tgcggtttcc cttcattcca acacaagcgc caacggggtc aaccctatcg tcgtacgact
    21781 caactgcaac cttggcgcgc tcgccgggaa tacgaacaat cttcttgatg gtaatcaggc
    21841 catcgaaaat ttcaggaacc tccagctcga ataggcgctc gaggaaggca ttggcagttc
    21901 tggaaaggat aatttgcggg gtgttgtttt tcatctccac cttgtacacc acggccctta
    21961 ttgagtcacc cttgtggtag aaatcggttg gtatctgctc tgttttgggc agcaccagct
    22021 cgttaccctc atcgtccaga accatgattt ctttcttcca gacctggtaa acctcaccgg
    22081 tgataaggtc tccaactcta tccttgtact tattgtagag gttgttcttt tcgagctcca
    22141 tgattcgtga agtgaggttt tggcgcaatg ccaggattgc ccttcgcccg aagtctgcaa
    22201 acttcacctc ggaggttacc tcctcgccca cctcaaagtc cgcatcaatt tttctagctt
    22261 ccgatagggc tatctgcctt cctggatctt cgaccttgcc gtcctcaacc acctcgcggt
    22321 tgtgccaaat ttcaaaatcg cccttatcaa tgttgataat aatatcgaag ttatcgtcag
    22381 acccgtaaag ctttataagc gtcgccctaa aaacatcctc cagaacgctc atcatggtag
    22441 gcctgtcaat attttttaac tccttaaact ccgcaaatgt gtcaactagg ttaatgtttt
    22501 ccattgtgct gattatttat catatttact atttaaacga aatgaccgct ttggttgact
    22561 ttaccacgtt gtagggaaac gactttcgct gctgcaccac ctgtttgtgc ttaccatcat
    22621 ccataagaac tttctcgcta tactcgatgc taaaattatc gctaccagcc tccacaataa
    22681 cgcctttgaa cttgtttccg tccaaaagca aaacctctac ctccctgcca atattgatgc
    22741 tatactgccg gacaacccta aacggctgtc ctaaccctgc agaggttacc tctaacccat
    22801 aatcttcaac gttcctatcg agcttcgact cgaccaaacg gctgattgcc acgcatgatt
    22861 taatactaac acccttttca ccatcaagca tgatggttat ggtatttttg ggacttatgg
    22921 agatatcaac aataaaaagg ttttcctcct ccagcttttc tgcaactatc gcttcaattg
    22981 tttcccggct aatcattatt gctacatcta ataaaacagg ggactctgtc ccctgccacc
    23041 tgcttaaaaa ctgttgcaaa tatacagctt tctaacattc tttcaaaata ttatgctgaa
    23101 tttatgaatt ttatcgggtt agttctattt aaagtgcgca ccagcatagc aactagtaac
    23161 gttttcaaat gttatgatga ctttgcctta acttgcaaat tgataaaatt tttgtgtatt
    23221 tagccaaaaa gaaaacatgc gcattgcggt taacacaagg ctcttgattc atggaaaact
    23281 cgaaggaata ggatggttta cctacgagac ctttagccgc atggccatag accatccaaa
    23341 cgatgagttt ttcttcattt ttgaccggcc gtttagccgg gagtttattt ttgccaaaaa
    23401 tattcaccct gttgtgcttg gtccgcctgc acgacatcct attctttggt atatctggtt
    23461 tgagttttcc gtaaatcggt ttttgaaaaa gctgaaaccc gatattttca tttctcccga
    23521 tggctacctg tcgcttaaag ccaagacacc ccagctggca gttattcacg acatcaactt
    23581 ttactactac cccaagggac tgccatggct gacctcgttg tacctaaaca agtatttccc
    23641 aaaatttgca ggtaaggcaa aacgaattgt caccgtaagt gagtattcaa aaaatacgct
    23701 tgttgaggct tacaaggtaa atccgcaaaa aatagatgta gtttacaatg gtgctagtga
    23761 taactttaaa cccctaaccg aaatagaacg agccgagcag cgcaaccagc ttaccgatgg
    23821 atggccctac tttgttttcg ttggtgcatt cagcccaaga aaaaatatta gcagcctcat
    23881 taacgctttc gatatgttta ggcgtaaaac aaacattcag gcaaagctgg ttctggtggg
    23941 tgaaacaatg ttcaaagctt cttccataga agcagcccac cataaaagta aatttaagga
    24001 tgatattatt tttaccggcc gaaaagggtt gcccgagtta agccgtattg ttggcgctgc
    24061 tcaagccctg atttttccgt cgctttttga gggttttggg atacctgttc tggaggccat
    24121 actgtgtgaa gttcctgttg ccgcatcaaa ttctacctca atgcccgagg tagcagggaa
    24181 tgccgcaata tactttgaac caaccagtga tgaatcaatt gctgaagcaa tggagcgtat
    24241 atggatggat acccagctaa ggcagagact catctccaac gcaagggtgc agcgccaaaa
    24301 gttcagctgg gagcaagccg ccagcaggct ttacgacatc attgctaaaa caatcgaaag
    24361 ttgatgggaa tgggatgttt gaggacaata cacacgccgg gactcgttga agcgggttgc
    24421 gatgaggccg gaaggggttg cctggcaggg cctgtttttg cggcagcagt aattttgcct
    24481 cccgattttt acgataactc cattgatgat tcaaaaaagc taaccctaaa gcaacggctt
    24541 gccgcccggc agctaattca acactacgca ttagattggg ctgtggcaag ggtgtcgaac
    24601 gaggaaatag acaggttgaa cattcttaat gcatccattc tggcaatgca catggcaata
    24661 aaggctctta ccagggcaac tccccagcat ttactggttg atggcaatag gtttaagaat
    24721 tttccaggaa taccccatac atgcatagtg ggtggagacc acacctacca gtcaattgcc
    24781 gcagcatcaa tacttgctaa aacataccgc gatgagctaa tgaatgaact ctcagaacag
    24841 tttccaggtt atggctggag aacaaacaag ggctacccaa ccaagctgca taggagggca
    24901 attgcagaac tagggccaac gccctaccat cggctgacgt tcgccctttt gccccagtcc
    24961 aagacaaaat gttagcttta aactaaacaa gagctagtct gtcataacac tatacttaac
    25021 cattaacaac caaaactcaa acaacatgaa aaaaagattt cttgcactac taaccggttt
    25081 gttgctttta accggtgcaa tggtcagggc cgatgagggg atgtggcttc tgcccctgct
    25141 caagcagctg aacattgacg tcatgcagca aaaagggctt aagctctctg ccgacgacat
    25201 ctaccagatt aaccagtcga gccttaaaga tgctgtaatc atcttcggtc gaggttgcac
    25261 cggcgaaata gtatccgacc aggggttgat tcttaccaac catcactgcg gttacggggc
    25321 catccagcag catagcaccc ctgagcatga ctaccttgcc gatggatttt gggcaaagtc
    25381 gttgcaggaa gaaattccca ccccggggct aaccgcaaca tttttagtac gtatcgagga
    25441 tgttaccgac caggtaaatg ctgccctaaa ccccagcatg accgaggctg aacgcaatca
    25501 ggccattcaa actgtaggca aaaaaatagc atccagcgct acagccggta cgcactacca
    25561 ggccagcgtt agaagtttct ttggcggcaa ccaattcttt ctgattgtta acgaggttta
    25621 caacgacgtc agaatggtag gtgctcctcc ctcgtcaata ggtaagtttg gtgccgacac
    25681 cgataactgg atgtggccac gccataccgg tgatttctca gtattccgcg tttatgccgg
    25741 caaggataac caaccagccg actactcacc cgacaacgta cccttgaaac ccaagcgatt
    25801 ccttcctgtt tcaattaaag gattacagaa tggcgatttt gccatgatta tgggttaccc
    25861 cggcagcacc gaacgctaca tgacctccgt ggaagtagag gagatgatga gcatctccaa
    25921 tgccaacaga atctacatta gagggattcg ccaggacatt cttctgaaag acatgctggc
    25981 cgacaaagcc gttaacatca agtatgcctc aaagtactcc ggttcgtcca actactggaa
    26041 aaactccatc ggcatgaaca agtccctcaa acgcctaaag gtgattgacg ttaaaaaagc
    26101 cggtgaagcc gaattcaccc ggtgggtaaa tgccaaccct gaaagagttc aaatgtacgg
    26161 ggaagcgcta aacctgatta acgaaggagt taccggtcga aaggactatc cccatgccac
    26221 ccaatacata ggtgaagcac tcagccgtgg aaccgagctg atgagcattg caggaatagc
    26281 cgaacgtatt gttgaaccct taaaatcggg aaacaacgag gagctaagca aaaatgtagc
    26341 tatgcttaag agtagagcta ccatgttcta caaggactac aacgccccaa ccgatagaaa
    26401 ggtggccaag gctatgttca aaattttttc cgaaaatgtt cctgtacagt accaacccga
    26461 tatcttcaac actattcgta caaagtataa gaacgatttt gataggttta tagatgaaat
    26521 gttcgacaaa tccatgtttg ttgactctgt aaagctattt gccttcctgt cgaaaccgaa
    26581 cctgaaaacc cttcaaaaag atcccgcatt cattgccgcc caatcaatta acgcaaaggc
    26641 aatggagctt tacgggttaa tggaaccctt caaccaaaag ctgcaaaaag ggcagcgact
    26701 cttcatagct ggaattatgg agatgaataa gggcaaggcg ctctaccccg atgccaactt
    26761 taccatgagg cttacctacg gcgaaataaa ggattactac cccatggatg ccgttcatta
    26821 cgactaccaa actactctcg atggcgtaat ggaaaaggag gatccggaca attgggaatt
    26881 tgtagtgccg caacgtctta aagacctata ccaggctaag gattatggcc gctacggcgt
    26941 aaatggcaaa atgcctatcg ccttcatttc aaccaacgac atcaccggtg gaaactccgg
    27001 aagccctgta atgaacggta atggcgaact cataggtttg gcattcgacg gtaactggga
    27061 ggccatgagc ggtgatatcg tattcgaaaa caacctgcag cgaacaattt cggttgatat
    27121 ccgctacgtg ctattcataa ttgataagta tgccggtgca accaggctgg tggacgagat
    27181 gcaaattgtg gagtagcaca gaatttccta tcggttttaa cccataataa tagcaaaggg
    27241 gatgccagta tttggcatcc ccttttcatc atcaaaccgc catataacca ttcggcaaaa
    27301 ccatttaatt acaaaattaa tccattcgtc aaaaatactt ttcacaaata caattttaaa
    27361 atgaatattg gttgacaaat tagggtaggt tacaccgttt taaacttgat gttttgcatg
    27421 caacctaact ccttagattt cactagctac tttttttaac agaagttttt tagggtaata
    27481 attgcaattt tattgttcta tttcataatt tagtaaaaat tttgtagcac tataaggtga
    27541 aaaatagcga agtactcatc agccataatg ggccactttc ctacgaggaa attggattct
    27601 tgctcaataa gatgacagcc atcctggaaa agtacaattt tggcattacc ataaaaaaga
    27661 aggtttatgc agccatggtg gaaagtttag aaaatgtgta caagcatcag gaccctattc
    27721 ccgaagatgc agggcatttt ccaaagttta cactctccct ggatgacaag tacatataca
    27781 taagcgtttc gaacccgctg ctcaaaacta aaattccgat gcttgaggac aggctggcga
    27841 gggtcaacca gctcgacaaa aacgggctaa aagagttcta caagcaaatt atccttaacg
    27901 gtaatgtttc acaaaagggt ggcgccgggc tgggcatcat caacattgcc aaagtaaccg
    27961 aaaacaagct cgacttcact ttttccgaaa ttgacggaca gcatgcctat tttacactgg
    28021 gtataaaggt tgcacattac acggaataat tcgaaaaaaa ctatggaacc actatacata
    28081 gaacccagcg agtttacacc cagagtcttt tttgaccctg gcaacaccgt atttgagatc
    28141 tcagggtttt caaggcccga aaatacagtt gacttttaca agcctatact tagatggctc
    28201 gatgaatacg aggagaatgt actaaacaag aacaccgagt atagcaaaag caacatggtt
    28261 cttaatctta aaatgaccta ttttaactct gcaacctcca agtacttgct cgacctgctg
    28321 atgaaattca tgaagttcca ttcaaaggga aatacggtcg agataaactg gttctacgac
    28381 gaggacgatg acgaaatcct tgaatcgggc gaagaaattt ccgaaatgat aaactttccc
    28441 ttcaactata tcccaataac cccaacccaa tagcttctta ttaacaacca aatcttttaa
    28501 aaacaggttt attcatgaac tctcttacca ttgaacccac tactatttca ccccatgtta
    28561 gctttgatat ggataagggg gtattcatca tttcgggata ttcacgtccc gaaaacgtca
    28621 gggacttcta tgcaccaatt atatcatggc ttgaattatt cagggatcag cttttccgtg
    28681 agcaatccga aggaaaaaca ataaacccca ttattttcaa cttcaacctt atttatttca
    28741 actcctcatc ggcaaaattt ctttacgata tcgtaatgct ccttgatgaa gttcaaaaga
    28801 agggcatcaa tctcaccatt aaatggcact acagtaaaga tgactccgaa ctccgtgagg
    28861 ctggtgaaga tctctcggat atggctcaag ttccatttga ttttattgag gtgtaagtta
    28921 atttatccac tccatagcga aatatggatg ttttcgtttt aattcccacc aatcaaacca
    28981 tcttgtaaaa actcccaggc gttactgcta cccgcaatag tatggactac aatacaagaa
    29041 ggccttcgac taataaatca aaacctgatt atttttcagc ccttgaacag gttttaatca
    29101 cccctacaat ttttttactg aaaatattct tacttttagt ttactttgct gggtatctat
    29161 ataaaagaca tttttggaag aatgctgaat aaatgattta ctaccccgat atgaatacaa
    29221 tactttatat tcgattaacc attgacaact ccaatcctgc catttacagg gacattcagg
    29281 taaaaagcca aacaagcctg gaggtgatgc acgaaatcat acaggcaaca tttgaatgga
    29341 gcaacctgca ctcgcactac tttaaggatt cagctggcaa catcattaag gacaagaaag
    29401 gtattctgat tggcagcatt attactacaa gcggaagtat tacctatgtg tacgattatg
    29461 ccgattcgtg gggggtcaga atagaggtgc tggaagcccg ggacatcatg gacgaaaatt
    29521 ttactccgat atgcaccgat ggtcgtagaa actcacctcc tgaagaatcg ggaggtattg
    29581 ttt
//
LOCUS       DAOPHX010000013        29355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_1543_length_29355_cov_6.620546, whole genome shotgun sequence.
ACCESSION   DAOPHX010000013 DAOPHX010000000
VERSION     DAOPHX010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 29355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29355
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1543_length_29355_cov_6.620546"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            446..1066
                     /locus_tag="PK502_01960"
     CDS             446..1066
                     /locus_tag="PK502_01960"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPG99613.1"
                     /translation="MFSGIVEEPATVVALRREMENLHITLKCSFAGSLKIDQSVSHNG
                     VCLTVVSREGDTYTVTAIRETLEKSNLGLLKVGDKVNVERSMRIDSLLDGHLVQGHVD
                     QTAVCTGVEEAEGSWYYTFEYDPSKGNITVEKGSIAVNGVSLTVVNSRSSSFQVAIIP
                     YTYEHTNFHQIQKGTVVNLEFDIVGKYITKMLRQYIESGQIANLSR"
     gene            1132..2193
                     /locus_tag="PK502_01965"
     CDS             1132..2193
                     /locus_tag="PK502_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG99614.1"
                     /translation="MNADRLIKSPQAVALIISVISALIAGVVSAAIFGVATEKQVLSC
                     VAIAMASMAGTFIASTILVSLFAARKIKPLYKILYQVDVKNSDIANEFEDNNLLENIN
                     DSLVSWTTSKTEEIDRLKEMERYRKEFLGNVSHELKTPIFNIQGYVLTLLDGGLADSH
                     INRKYLERTEKSINRMIGIVEDLEIISKLEAGELKLNMERFNIVGMVEDIFEALEERA
                     RKKSISLEFDKVYERPVWVYADKKRIYQVLHNLIINSINYGIQHGHTVVAFSDSGKRV
                     TTWVTDDGIGISQKDIPRIFERFYRVDKSRSREQGGTGLGLAIVKHIIEGHKQSIKVE
                     SSLNKGTTFSFTLDKPTKQ"
     gene            complement(2255..3268)
                     /locus_tag="PK502_01970"
     CDS             complement(2255..3268)
                     /locus_tag="PK502_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPG99615.1"
                     /translation="MILVTGGTGLVGAHLLYELTSRGESVRALKRERSNTEFVDWVFK
                     LYSHESSKLLQGIEWVDGDLLDYQSLLDATKGVQTVYHTGGIVSFNPLQSSSVIETNI
                     TGTGNLVDACMDNGVKEICHVSSIAALGQANESGMVDEDCRWIESKGQNAYAVSKLYG
                     ENQLWRGFAGGLRVIVVNPTVILGPGRWDSGSGQLFKRVYKGMPFYTNGVTGFVDVRD
                     VARAMVVLMEETGINGERFILNSENVSYRDIFTMMAASLGKRKPSIRVPAGVVDAVYP
                     LVQLFGQLSGRGNLVSKANLKTAFRVTLYSSQKIREKIGFEFIPIEETIRFVGKAFRN
                     AEP"
     gene            3509..5203
                     /locus_tag="PK502_01975"
     CDS             3509..5203
                     /locus_tag="PK502_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPG99616.1"
                     /translation="MASENLNEELASERTKNFLEEIIEEDIKSGKHGGRVLTRFPPEP
                     NGYLHIGHAKSICLNFGLAQRYGGKTNLRFDDTNPEKEDVEYVNSIKEDIRWLGFQWA
                     GEYYASDYFEQLYEWAEVLIKKGLAYVDDQTQEEIRINRGTVNQPGKPSPWRNRSVDE
                     NLDLFRRMRAGEFGDGERVLRAKIDMAHPNMLMRDPIMYRIIHAEHHRTGSKWCIYPM
                     YDYAHGQSDSIEKITHSICTLEFDVHRVLYEWFIEKLEIFPSRQYEFARLNLSYTVMS
                     KRKLLELVREKIVMGWDDPRMPTICGLRRRGYTPESIRYFADKVGVAKRDNVIDLSLL
                     EFCLREDLNKRAERRMAVLSPLRVLITNYPEGQVEELDAINNPEDDAAGTRSVPFSRE
                     IYIEQDDFMENPPKKFFRLAPGQEVRLRYAYFIRCNEVIKDEQGNIIQLNCTYDPASR
                     GGNSPDGRKVQGTIHWVSAQHAIKADVRLFDRLFSKPDPNDVPEGVDWKSTINSDSLR
                     VVEAYIEPSLKNVKPLDKFQFERLGYFSVDYDSTSQKLVFNRTVTLKDTWAKIAGK"
     gene            5309..5381
                     /locus_tag="PK502_01980"
     tRNA            5309..5381
                     /locus_tag="PK502_01980"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5342..5344,aa:Gln,seq:ctg)
     gene            complement(5390..6862)
                     /gene="cysS"
                     /locus_tag="PK502_01985"
     CDS             complement(5390..6862)
                     /gene="cysS"
                     /locus_tag="PK502_01985"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPG99617.1"
                     /translation="MDSKLHLYNTLSRTKEEFKPINPPHVGLYVCGPTVYGAPHLGHA
                     RPAITFDLLFRYLTHLGYRVRYVRNITDVGHLENDADEGEDKIAKKARLEQLEPMEVV
                     QRYTDLYHDAMRKLNVLPPSIEPRASGHIIEQIELIKRILDNGYAYVRNGSVYFDVAR
                     YNERYPYGKLSGRRIDDLMANTRQLDGQEEKQNPFDFALWKSADPAHIMRWPSPWGEG
                     FPGWHMECSAMSIKYLGQPFDIHGGGMDLLFPHHECEVAQSVAASGHEAVRYWIHNNL
                     ITINGQKMGKSLGNFITLEELFTGSHKLLQEAYSPMTIRFFMLQAHYRSTLDFSNEAL
                     QAASKGFARLMAAIRTLDRIPPSIKSTVDVSDLQNRIYEAMNDDLNSPVAIAALFEWV
                     KIINLLAEGKETISAADLETLGSMFRTFTFDILGLVDEQQSASKHTELAGKLIELLLA
                     MRLEAKRQKDFATSDRIRSELEQLGVVVRDRKDGFDWEIS"
     gene            complement(7462..7893)
                     /locus_tag="PK502_01990"
     CDS             complement(7462..7893)
                     /locus_tag="PK502_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99618.1"
                     /translation="MATCWVLITVSAVSAQTANADAEKALKAEKVAFITSKLELTPKE
                     AKKFWPVYDAYWDKKNEVLRERRALTGELTKIIEQSPDDKTLLAYADRYVENRQRETE
                     LLVELNEELKEILPPRKILLLYKANHEFKNYLLQKVQETGK"
     gene            complement(7914..8378)
                     /locus_tag="PK502_01995"
     CDS             complement(7914..8378)
                     /locus_tag="PK502_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99619.1"
                     /translation="MEAKKNGLEVIEGKAGKELPFSVPDGYFDSLPRRIQERCTQPQH
                     QQPKQSWIFALRSQLALAAGFALLVVAAYFGYYTTTRSIPRDSYTLQKSDYLNIVSRS
                     ISEFDDIDLYKAIENKRKTDSIDSAARTLQQRYFIKKGNATTIIGEKYEEKP"
     gene            complement(8430..8978)
                     /locus_tag="PK502_02000"
     CDS             complement(8430..8978)
                     /locus_tag="PK502_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPG99620.1"
                     /translation="MECYSDKELLEMFRNGETRNFAFNLIVNKYQERLYWHIRKIVVG
                     HDDADDVIQNTFIKVWGGLETFREESQLFTWLYRIATNEALTFLKKKKVKSFLSMDDV
                     ESQLAKTLETDAYIDGNELQLKLQKAILKLPEKQRLVFNMKYFEEMKYEEMSEILGTS
                     VGALKASFHHAVKKIEKFMGEN"
     gene            complement(8986..9942)
                     /locus_tag="PK502_02005"
     CDS             complement(8986..9942)
                     /locus_tag="PK502_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPG99621.1"
                     /translation="MKYTSTGNKDTRSGFGAGLYELGKVNPNVVALCADLIGSLKMED
                     FSREFPDRFFQTGIAEANMIGLSAGLTIGGKIPFAGTFAVFATGRVYDQIRQSVAYSN
                     KNVKICASHAGITLGEDGATHQMLEDIGLMKMLPNMTVINTCDYNQTKAATIAIANHV
                     GPVYLRFGRPAVPNFTPADQQFTIGKAVKLTEGTQVSVFATGHLVWKALEAADMLEQQ
                     GISAEVVNIHTIKPLDEEAIIRSIQKTGCAVTAEEHMMNGGLGDSIAQVVARIHPVPL
                     EMVAINDTFGESGTPEELMVKYGLDAPSIVKAAKRAIARKNN"
     gene            complement(10071..10469)
                     /locus_tag="PK502_02010"
     CDS             complement(10071..10469)
                     /locus_tag="PK502_02010"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HPG99622.1"
                     /translation="MKNIALIAHDAKKPELARFLKDHEEWLPGVNLLATGRTAEFLEE
                     QGIKCRHLSPGQSGGYNEITGMIQRGEVDIVIFLRDPKVVQQHHEDIRLLLEACNIKN
                     IPLATNKATAELVILGLIHMEAIERMKRNK"
     gene            complement(10491..11336)
                     /locus_tag="PK502_02015"
     CDS             complement(10491..11336)
                     /locus_tag="PK502_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPG99623.1"
                     /translation="MTDFEFLNKMAAQVRRDVIRMVHRPSSGHPGGSLGCADFFTALY
                     FDVLRADPKHFTMDGTGEDLFFLSNGHLSAAWYSVLARRGYFDVKELSTFRLLNSRLQ
                     GHPTPAEHLPGIRIASGSLGQGLSVGCGAALAKKLNGDSHLVYTLHGDGELQEGQIWE
                     AALFAAAKGIDNLIATVDYNGKQIDGPVDTVVGLGDLKAKWLAFGWDVLETNGNDMPA
                     LVKTLHEAVEHTGKGKPVIILMRTQMGMGVDFMVGNHKWHGKAPNDEEFAKAMAQLPE
                     TIGDF"
     gene            complement(11449..12264)
                     /locus_tag="PK502_02020"
     CDS             complement(11449..12264)
                     /locus_tag="PK502_02020"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HPG99624.1"
                     /translation="MVNLQEIIDAAWENREMLGTPEVNDAIKSVVEMLDKGEVRVAEP
                     ADDGWKVNEWVKKAVLLYFSICSFQATGAGPLEFYDKIPLKKNYQSLGVRVVPHAVAR
                     YGSYIARGVVMMPSYVNIGAYVDEGTMVDTWATVGSCAQVGKGVHLSGGVGIGGVLEP
                     VQAAPVIIEDGCFIGSRCIVVEGVRIGRESVLGANVAITRSTKIIDVSGSQPVEYKGM
                     VPPRSVVIPGSYTKQFPAGSYQVACALIIGKRKESTDLKTSLNNALRDFGVSV"
     assembly_gap    12336..12345
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12488..14887
                     /locus_tag="PK502_02025"
     CDS             12488..14887
                     /locus_tag="PK502_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPG99625.1"
                     /translation="MRKLMLLSVLLVVSLGLQSQELVRYQVPPAEMVAILEAPPTPGF
                     LLSPDGKHALLSYPQVLPDLAELAKPELKLAGFRVNPENFGRYDSRIFDRLELLNISN
                     KMVTSLSGIPDSGHITSFSWSPDGKNVAISVYFNSSVELWIVEVESGVARPLSASLNE
                     SVISPGFAWHPDGKSLYVLKHREGMKKPGREKLPLGPSVQQATGQKSQVRTFQDLLKS
                     PADEELFEYYATSQLYRVNLLGEASEVGNPGIISQVEPSPDGSYIYVEQVVRPFSYIV
                     PAQYFPRLCHIWNADGHLVKRLAEQPLADDIPSGFSAARKGKREMQWRSDVPSTIVWV
                     EAQDDGDPHRDVKIRDVVYSLDAPFRGEPLKLVELETRFSSISWGWSNLAVAYGRWWS
                     SRRQTASLFNPSAPTDRPRVLWDRSWEDAYTDPGQLVMVNNANGFSVLQTNSSRTKLY
                     LSGKGSSPEGDMPFLDEINLKNLSVKRLWQCHAPLYEYFIGFYDMDNSRIVTSQESVN
                     RQPNFFIRDIKRQKLVQLTNFPDPYPGLHGVHKELVTYRRPDGVTLTGTLYLPAGYEK
                     GTRTLPTLMWAYPQEFLSADFAGQVTGSPYRFIRPSVLSPILWVVRGYAVFDNMSMPI
                     VARNGGEPNDTFIEQLVENAKAAVDTLVGMGVANPQKVAIGGHSYGAFMTANLLAHSD
                     LFAAGIARSGAYNRTLTPFGFQNEERTYWQAPEVYNRMSPFMHADKINEPLLLIHGEN
                     DNNSGTFPLQSERLFAAIKGHGGTARLVMLPYESHGYRARQSVFHTIWEMDCWLEKYL
                     K"
     gene            15103..15906
                     /locus_tag="PK502_02030"
     CDS             15103..15906
                     /locus_tag="PK502_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99626.1"
                     /translation="MVGFRKIWVLIFFVVFCKVSFSQEKLLPFDTLSIVHFRVINANT
                     REPVERAYILNLTYNWGSISDLLGYSYLPIHFGDKLKISAIGYYTQELICVGQFKADS
                     AFYEIKLKPRVYELNEVKITKFSTYERFLRGVINLNIPKTRDDKQLEVIEKYLFGVVK
                     KMDLKNTPTMASGIAFGKDWYAKQNEKLAEQLEREKTRRVIDEKFNPGLVQKHTGLSD
                     KELYEFIGYLDFSDEFLLQATDYEIHQKIMEKYNVFMAGKSNELKNQEK"
     gene            15903..16886
                     /locus_tag="PK502_02035"
     CDS             15903..16886
                     /locus_tag="PK502_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPG99627.1"
                     /translation="MSEENSSCYQPEASDVVSAYELIKDKVKHTPVHSSASVNALLGA
                     TLFFKCENFQKVGAFKYRGATHAVLKLNDEEARKGVATHSSGNHAAALALAAANRGIP
                     AYIVMPENAPAIKKKAVAGYGGIITYCRSTQQDREETLRVVQQQTGATLIHPYNNINV
                     ICGQGTAALELLDEMPDLDVVIAPVGGGGLLSGTATFIKGQYPHVKVFGAEPINADDA
                     FRSLRDGKIYPSLSPSTIADGLLTSLCPLTYSILRSKCDGIFTVSEKGIVNAMRLIWE
                     RMKLVVEPSSAVPVGVIAEHPEAFRGKRVGIIVSGGNADLDHLPWLDQSNG"
     gene            complement(16876..17961)
                     /locus_tag="PK502_02040"
     CDS             complement(16876..17961)
                     /locus_tag="PK502_02040"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPG99628.1"
                     /translation="MAKGGILHKVIVAANILFALGLLFCYASVYINPARFWIPAIVGL
                     FYPFFLLANIAFLTYWIIVWKKELLISLLAILLGINHLSAFVQLRPGNKHESAVPSIR
                     LISYNVNLFRLYPWSKLPPSYDGVIRYIGSNNFDIACLQEFYTVSTKFSEADAKRKLG
                     MRSKIGYIIQNSRSAYGLAIFSRFPIVNSGEIKFENTLNSSIYADVKINGDTVRVYNL
                     HLQSLRLKEHNFNFLLNNELRQGNDRYDEIMDLASRLRIAFIKRSQQVELVNRHLKNC
                     PYPVILCGDFNDTPVSYTYRELTGKLHDSFKEAGAGMLNTYAGILPSYRIDYILHSPE
                     FKTLKFSSPRLNLSDHYPVCANFTLTH"
     gene            complement(17963..18850)
                     /locus_tag="PK502_02045"
     CDS             complement(17963..18850)
                     /locus_tag="PK502_02045"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPG99629.1"
                     /translation="MSISKEIKDSFRFGSSLTQLIYINLAIFLIYSLVRVIMFIGGTQ
                     PGSSYAHWLAVPAGLHQLLSRPWTLLTYMFFHENFLHILFNLLWLYWFGRLFLQHLSQ
                     RQLVGVYLLGGITGAIFFIAAYNILPVFRAISSEAMALGASASVLAIVIAVSTLKPNN
                     SIYILFLGEVKLKYLALATIILDIISIPVSNPGGHIAHLGGAVFGFSFIKLLNRGTDL
                     TKFLNIFSRVKKPKAHRSKNLKVSYRRPETDYEYNRRKANEQKSIDRILDKIAKSGYD
                     SLSKEEKETLFRMKNDSVN"
     gene            complement(19037..19858)
                     /locus_tag="PK502_02050"
     CDS             complement(19037..19858)
                     /locus_tag="PK502_02050"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPG99630.1"
                     /translation="MSLSRSGFLSSIPPVVKNLILINTVIWLVTWMLQRYGVDLNAML
                     GLYTPGSPHFKPYQVVTHMFMHGGFTHLFFNMFALWMFGRILEQVWGGKRFLLYYMVT
                     GLGAAAVHSLVNYLQFSSIQADSIAMLNTLSPDTFAAFVTKHFPEFYNSIYSDFLDKW
                     FQNPSNPIMIDQAQQLSQQLLSLQLNIPTVGASGAVYGVLLAFGMLFPNTVLMILFPP
                     IPIKAKWMVILYGGLELFLGLTQAGSNIAHFAHLGGMIFGFFLIKYWQSRKNVFY"
     gene            complement(19929..21773)
                     /gene="mutL"
                     /locus_tag="PK502_02055"
     CDS             complement(19929..21773)
                     /gene="mutL"
                     /locus_tag="PK502_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPG99631.1"
                     /translation="MADIIHLLPDSIANQIAAGEVVQRPASVVKELLENSIDAGCKAI
                     QLIVKDGGKTSIQVVDDGIGMSETDARLCFERHATSKIKEAADLYSIRTMGFRGEALA
                     SIAAVAEVELRTRRLSDEVGSMVQVGGSEIVAQQPVSCPVGSTLTVKNLFYNVPARRK
                     FLKSNSVELKHIISEFQHVALAFPEISMSLSHNGSEIYKLAPSNLKQRIVNLFGKSIS
                     SQLVDCVAETSIVAISGYIGKPESARKSPGEQFFFVNGRFMKSPFLHKAVVNAYNRLL
                     PANSIPTYFIYFRVDPGSIDVNIHPTKTEVKFEDDGAVWQIVHAAVREALGKFAVAPS
                     IDFSSDPGFSIPHFPKDSPVELPEIELNPNFNPFEDEGARFAQRTREHNFTPQQPVKG
                     WEQLYQDVNPNFNFEPKQTAVQTFASKGFDDSVAENTGRFFQIKGKYIITAVKSGMLV
                     VDQKRAHERILYEQFYKNLNLEKNVAQRELYPQTIELNPQDFETIITNQENFLRLGLD
                     ISNLNHNTISINSLPGNTRITDPALFLENLLAIINENEALPFDSAKEKIALSMAKAAA
                     VGYGKRLSESEMQEVVDQLFACREPNYTADGKKTIALIEMSEFEKWLK"
     gene            complement(21788..22012)
                     /locus_tag="PK502_02060"
     CDS             complement(21788..22012)
                     /locus_tag="PK502_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99632.1"
                     /translation="MVNNFFFGAQEHRDTIFWVKTHKGIVRGSVDRANSAFTPERMGA
                     MWIRHPDSYLPTRNILEVLTILLLVAHLIF"
     gene            22221..22868
                     /locus_tag="PK502_02065"
     CDS             22221..22868
                     /locus_tag="PK502_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99633.1"
                     /translation="MKPAGAVELIILTAASMLNGCTKNVEPFPTSATLSPSLSVPLGE
                     GGVSLTKTLQTLGIPVVNLSEDVPQWATYGFVYVADTIPLNLTEVYNRGDSITYLMVR
                     TNIWNQFPLGGRAQVFFLDANNQVIDQLYEDMVSVGPAEHGSHGEVVNTAFESNETSF
                     NTSRISLLSAAQKVVIYAGLEITDEGVSQSNITYFDQYQLRVQVALQVDFIMNLN"
     gene            22884..24311
                     /locus_tag="PK502_02070"
     CDS             22884..24311
                     /locus_tag="PK502_02070"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HPG99634.1"
                     /translation="MRRIVNLLIFFSAYSTIVCAQQDNTLFLLHNIPQSNFTNPAVQI
                     QCPVYVGVPLLSSVHFNVNSTGFSYGDVANGSGVANLNALVAGMHRWDFVSEEVHFTP
                     VSFGFMYDRHRYISFAWTERVETKVFLPKKLLSLSVDGNTQYVGDELKTGNPGANAVY
                     YREFSVGYSKKVNSGLILGVHGKLLFGLAGAFTRRRPVNVDVDAITHNIDAQWNPKVD
                     VAYPLGVATDASGNVSNVTVGGFSPLNFFLNFGNQGIAADLGFIWQHDDVTWSGSLLD
                     LGFIWWHKQTTRFENSGHFVYRGATPADLDNPDDYINQLGDSLNNQIQVEYSRKGFIT
                     LLNPKIYLGASYPVSERFNVGAHVRAEWYPGRPVMGITLSGMFFTKKGGSAALSYSVM
                     NGSFINVGAGLGWGGQTFQFYVVSDNLMAALYPEKARNANLRFGFNLFFGCIEKKKKV
                     EVPHGNGCGCYWSWDDKTRRKLGRN"
     gene            24575..25111
                     /locus_tag="PK502_02075"
     CDS             24575..25111
                     /locus_tag="PK502_02075"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPG99635.1"
                     /translation="MKKICRIFAIAVWMAPLGLMAQPPEVGQVAPDILLSSPTGDSIR
                     LSSLRGKLVLVDFWASWCSPCRRENPNLVQTYNKFKDACFENGNGFTIYSVSLDQKQD
                     AWLKAIADDGLVWPYQVSDLKGWRSEAAKQYGVRAIPMNYLINANGVVVATNLRGDEL
                     DSTLRKLRKWRIFSNECI"
     gene            25143..28259
                     /locus_tag="PK502_02080"
     CDS             25143..28259
                     /locus_tag="PK502_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPG99636.1"
                     /translation="MKVGHILWKTAKIILITLGSLILLVVILLAAAKLSENKIVGLVL
                     NKVSVELKAPFSVREVNLLPLRNFPNLTVELQDFSVGKSTDSLQSGQSSNPFDTLFSL
                     HKLYVAVKAKPLLSSKVVVDQVEIDGATLNYHVDSTGASNFDFLMTTTDTTQVPEDTS
                     QVETVLNILLHDLTLSNIVLNYADDSLRAKACVAIPEIKLNGKVMGSYYAGAVEGSIS
                     VSDLALEGTHADRMGKTSLDFKVGYDNGDISVDKLNLNADGFGLSANGKAKLSDSIFV
                     DMGVGLTNMDFGRLTAYLPDTLLSSYGLTALGGIFDLKAHIKGFVYDTLLLPSVEASL
                     DFRNGFIKTTSYPTVETLAFNGKVKLPDPNNLKTASADFSTFRVKTHASNLNLAFKIL
                     NIEKPIYNVKGDMFITLDEFGSFIPDSLVKSLSGNIMARIETRGQLPDSLGMSSADYF
                     MQNTTLNVKLRNITAVLDSSTSVRGVNLNFDFLPGKQVAVENFTADLSIPGYHVGLDN
                     FLLRARLIGDIANTSKFGVDVDSLLIKVGNSSLGGRFKVMNLDKPTYSAKSFVNVNFN
                     ELRPFIPDTLVETLTGRMAATVQSHGTLNLDSLSSQIMPIAFEQSRFTLNLDSLNFAM
                     PNDTLMRVDNLSLKLAMSDDTIRINGFHGQAMGIEFAIDSTRIWNVYKAMLKGEKDKK
                     LVADTRIQLSDIDFAMFAPFMATDSTAAEPTPQPAEPQEKVAGSEAPATESSFTIPSY
                     IIRGTVAVNSFKYGNMLIDKISTKFRVDDSLYVIDDLKLNAFGGDLTLSALYDTRNDT
                     ATVIEAKCNLNRMNIRQLLADNNDFDQTFFTHENIEGLATGELFARVIMRDTNILYDK
                     INLLGNFKLENGGIYNFEPLMQLSKFTNLNELERVVFRTLETSVFVYNNEIYFPKTDI
                     VSSAMDISVYGMQSFADDYEYHLIVHLGDVLVGKSNKLLKQQGMESDVFEGQDKAKRS
                     GLYLVALNRGKDSKYGFDTQHLQRLMKTTIRVQERGLNLIFNPKVVNFSTEIDRRERK
                     KKPNEAKG"
     gene            28243..28917
                     /locus_tag="PK502_02085"
     CDS             28243..28917
                     /locus_tag="PK502_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPG99637.1"
                     /translation="MKLKVKLDRHHLIAYAISLVLVVLIGYKLWRYFTPDSKEAVLQE
                     SPEPRPLDIVFGSDTASLPVYMYASYSCTFCRQFFTDVFPMLKKEFLDNGRVKLVMRL
                     LENTNHPDALNSAKTAVCINRYGYFDKLHELLLTDSRVIYTPEFRDMVNDFTARDELV
                     AECILGGQADEYILQIRNEFRKFGLSGTPTFIINRNVYRGYRSYQEFRKIILHHMETE
                     DRKGIN"
     gene            28988..>29355
                     /locus_tag="PK502_02090"
     CDS             28988..>29355
                     /locus_tag="PK502_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99638.1"
                     /translation="MKNKIYLLPLSLLAVFTVVTWSCQKDDDEDVCQKFDPPQCEIAN
                     ACCPTDGGNCYYEYGDQKFYCDKSKATADDPDGCNAAEDQVIDVICSTAKGADIQLAK
                     VQLRELTRKLMNEARMLSVC"
BASE COUNT         7594 a         6802 c         7128 g         7821 t
ORIGIN      
        1 actgccctgc taccgcacaa gtctccccgt gtggtggaga gcagagcgta agtggtagta
       61 gagaatagga aagggagcgg tagccgctat taaaaagcta atccggcagg gttatgcttt
      121 gtaagcttaa taattgggag tgttgagtgc tctggttgag gggtacggtc gagattgcca
      181 cacgtcggga gacgtgaggc agctaaagaa ccattacctt gacaggtagt ggggtgcatt
      241 ataccctact cataccaaga gggttagcct tttttttaaa gctttgcctt gcagaggtca
      301 tccttggcaa ggtgtggtcg agctgctttt tgtcccgcac tttcaaggca ttttttgtat
      361 ttttgcagtg tatatcagct agctcttatt tttaaaacct agctggtaag ggtttcgtag
      421 ttgaactacc taaatcaaat taatcatgtt ttcaggaatt gttgaagaac cggccacggt
      481 ggtcgcccta cgcagggaaa tggaaaacct gcacatcacc ctgaaatgct cctttgcggg
      541 gagtttgaaa attgaccaga gcgtttcgca caacggggtt tgccttacgg tggtaagcag
      601 ggagggcgac acctataccg ttacggccat aagggaaacc ctggagaaat cgaacctggg
      661 gttactaaag gtgggcgata aggtgaacgt ggagcgtagc atgaggatag acagcctgct
      721 tgatgggcat ctggttcagg ggcatgttga ccaaaccgct gtgtgcacgg gtgtggagga
      781 agccgagggc agctggtact acacctttga gtacgaccct tcaaagggga atataacagt
      841 tgaaaagggc tccattgccg ttaacggggt tagcctcacc gtggtgaact cgcgcagcag
      901 ctcgtttcag gtggccatta ttccctacac ttacgagcac accaactttc accagataca
      961 aaaggggact gtggtgaatc tggagttcga catcgtgggt aaatacataa ccaagatgct
     1021 tcgccagtac atcgaatcgg gtcaaattgc caacttatcc aggtaggcca tgaagggagc
     1081 tgtcaggttt tacaacagag ctgtggtttg ggtaacgaat aacccattca aatgaatgct
     1141 gatcgtctga ttaaatctcc ccaggcagta gccctcatca tttcggttat ttcggcctta
     1201 atagccggtg tggtatcggc tgccattttc ggggtagcca ccgaaaagca agtattatcc
     1261 tgtgtggcca tcgccatggc atcgatggcc ggcacattta tagcttccac aattctggta
     1321 agcctttttg ccgcccgaaa aatcaaaccc ctatataaaa ttctatacca ggtggatgtg
     1381 aagaattccg atattgccaa cgaatttgag gataacaacc tgctcgagaa tatcaacgat
     1441 agtctggtaa gctggacaac aagcaaaacc gaggagattg accggttaaa ggagatggag
     1501 cggtaccgaa aggagttttt gggaaacgtg tcgcatgagc tgaaaacgcc aattttcaac
     1561 attcaggggt acgtgctgac gctgctcgac ggtgggctcg ccgattccca tatcaacagg
     1621 aaatacctgg agcggaccga aaaaagcata aaccggatga tcggcattgt ggaggatttg
     1681 gaaattatct cgaagcttga ggctggtgag ctaaagctga acatggaacg gttcaacatt
     1741 gtcggcatgg tagaggatat ctttgaggcg cttgaggagc gggccaggaa gaaatcaata
     1801 agccttgagt tcgacaaggt ttacgaaagg ccggtgtggg tttacgccga taaaaagcga
     1861 atttaccagg tgctgcataa cctcatcatc aactcaataa actacggaat tcagcatggc
     1921 cacactgttg ttgcgttttc cgattcgggc aaaagggtaa caacctgggt aaccgacgat
     1981 ggaataggca ttagccagaa ggacattccg agaatttttg agcgtttcta cagggttgat
     2041 aaaagccggt cgcgcgaaca gggaggcaca gggcttggac ttgccattgt gaagcatatc
     2101 atcgagggtc ataagcagag cattaaggtg gagagtagcc ttaacaaggg gacaacattt
     2161 agcttcaccc ttgacaagcc aaccaagcag tgatgtggaa acaaagtatg ctgcaaccca
     2221 attgtcctaa agccagtgta gaataattgt tgcgctatgg ctccgcgttc ctaaatgcct
     2281 ttccaacaaa cctgatggtt tcttcaatgg gtataaactc gaacccaatt ttttccctta
     2341 ttttctgcga ggagtacagg gttaccctga aagccgtctt taagttggcc ttggaaacga
     2401 ggttccctct tcctgagagc tgtccaaata gctggacaag tgggtaaacc gcatccacta
     2461 caccggctgg caccctgatg ctgggcttgc gtttgcccag cgaggcagcc atcatcgtga
     2521 agatatccct gtagctgacg ttctcggagt taaggataaa cctttcaccg ttgatgccgg
     2581 tttcttccat gagcaccacc attgcccttg caacatcacg cacatctaca aagcccgtaa
     2641 cgccgttggt gtagaaaggc atgcccttat atacccgctt aaagagttgg ccgcttccac
     2701 tatcccagcg tcccggccca agaattaccg ttgggttaac aacaataact cgcaatcctc
     2761 cggcaaagcc tcgccaaagc tgattctccc cgtacagctt gctgacagcg taggcatttt
     2821 gccccttact ctctatccat cggcaatcct catcaaccat tcccgattcg ttggcctggc
     2881 ccaatgcggc tatcgagctt acatggcaaa tctcttttac cccattgtcc atgcaggcgt
     2941 caaccaaatt gcccgttccg gtaatgttgg tttcaatcac gctcgagctc tgcagggggt
     3001 taaacgaaac aattccacca gtatggtaaa ccgtctgaac accctttgtt gcatcaagta
     3061 gcgattggta gtcgagcaaa tcgccatcaa cccactcgat tccctgtagc agcttgctgg
     3121 actcatggct gtaaagcttg aatacccaat caacaaattc agtgttggag cgctcacgct
     3181 tcaacgcccg cacgctttcg ccgcgtgaag ttagctcgta tagaagatgc gcccctacca
     3241 gtccggttcc cccggttacc aaaatcatac tatttccatc tgttggtttg ctattaagaa
     3301 caagtattct gcacccaggt tcaaacatag caaaatttca gctaaattgg taaaaatcag
     3361 gaatgttgaa gatttacggg tgatttgaag ctgccattat atgggcggtt acgtctaaac
     3421 ttaagggaag gagtgtaaaa tttattaggt gctaataaac tttagcggca aatttctact
     3481 tttgcttctg tgaatcaacc caaatatgat ggcaagcgag aacctaaatg aggagttagc
     3541 ttccgaaagg actaaaaatt ttctggagga gataattgag gaggatatta agtcgggaaa
     3601 gcatggcgga agggtcttaa cccggtttcc accagagcca aacggctacc tgcatattgg
     3661 ccatgccaaa tcaatatgcc tgaactttgg gcttgcccaa aggtatggcg gcaaaaccaa
     3721 cctgcggttt gacgacacca accccgagaa ggaggatgta gagtatgtaa actccatcaa
     3781 ggaggacatc aggtggctgg gattccagtg ggcgggcgag tactatgctt ccgactactt
     3841 tgaacagctc tacgagtggg cagaggttct cattaaaaag gggctagcct atgttgacga
     3901 tcaaacccag gaggaaataa ggataaaccg cggcacagtt aaccagcccg gtaaacctag
     3961 cccctggcgt aaccgatcgg ttgacgaaaa tcttgacctg ttccgtcgga tgcgggccgg
     4021 ggagtttggc gatggcgaaa gggtgctccg tgcaaaaatt gacatggcgc accccaacat
     4081 gctcatgcgc gacccaatca tgtacaggat tattcatgcc gagcaccatc gaacaggtag
     4141 caagtggtgc atttatccaa tgtacgatta cgcccacggg caatccgact ccatcgagaa
     4201 aattacccac tccatctgca cacttgagtt tgacgttcac cgcgtgctgt acgagtggtt
     4261 cattgaaaag cttgaaattt tcccctcgag gcagtacgag tttgcccggc tcaacctctc
     4321 ctacacggtg atgagcaagc gtaagctgct ggagctggtt agggagaaga ttgttatggg
     4381 gtgggacgat ccaagaatgc ccaccatttg tgggctacga cgcaggggct acactccgga
     4441 gtccatccgc tactttgccg acaaggtggg ggtggccaag cgcgataacg tgatagacct
     4501 ttcacttctg gagttttgcc ttagggagga tttgaacaaa cgtgccgaac gacggatggc
     4561 ggtgcttagc ccgctgcggg tgctgattac caactatccc gaggggcagg tggaagagct
     4621 tgatgctatt aacaaccccg aggatgatgc ggccggtact cggagtgtgc cattcagtag
     4681 ggagatctac attgagcagg atgactttat ggagaatccg ccgaaaaagt tcttccggtt
     4741 ggcgccaggg caggaggtac ggcttcgata tgcctacttt ataaggtgta acgaggtgat
     4801 taaggacgaa cagggtaaca ttattcagct caactgcacc tacgacccgg cctcccgagg
     4861 tggaaattca cccgatggta ggaaggttca gggaaccatc cactgggtat cggctcaaca
     4921 tgccattaaa gctgatgtac gcttgttcga caggcttttt tccaagcccg accccaacga
     4981 tgttcccgag ggggttgact ggaaatcaac cattaattcc gactcgctca gggtggttga
     5041 agcctacatt gaaccctcgt tgaagaatgt taaacctctc gataaattcc agtttgaaag
     5101 gcttggctac tttagcgtgg attacgactc tacatcccag aagctggttt tcaaccgcac
     5161 cgttaccctg aaggatacat gggctaaaat tgcggggaag tagcaaaggc gaaaagccta
     5221 ttgcggcttg gtaggtcatg gggaataaaa attttcccgg tagaattacc agtaaaaggc
     5281 taattagcta tatttgcacc gtaaaaattg tcctgtggtg taattggcaa cacatctgac
     5341 tctggatcag aagagtccag gttcgacccc tggcaggaca acattttttc tagctaatct
     5401 cccagtcgaa cccgtcttta cggtcacgca caacaactcc cagctgttcc aactcgctgc
     5461 gaattctatc ggatgttgca aagtccttct gccttttagc ctcaagcctc atggctaaga
     5521 gtagttctat taacttgcct gccagctcag tatgtttgga ggccgactgt tgttcgtcca
     5581 ccaggcccag tatgtcgaag gtgaaggttc ggaacatcga gccaagcgtt tccaaatcgg
     5641 ctgcagagat agtctccttc ccttctgcca gcaggttgat gatttttacc cattcaaata
     5701 gcgcagcaat ggccacaggg ctattcaaat cgtcgttcat ggcctcataa atgcggtttt
     5761 gcaggtcgga cacatcaacc gttgatttga tggaaggggg aatcctgtcc agtgttctaa
     5821 tggcagccat caaacgggca aaccctttgg aggcagcctg cagggcttcg tttgaaaagt
     5881 cgagggtgga gcggtagtga gcctgaagca tgaagaacct tatggtcatg gggctatacg
     5941 cctcctgcaa aagcttatgc gatccggtga atagctcctc gagggtgatg aagttgccta
     6001 gcgacttgcc catcttctgg ccgttaatgg taatcaggtt gttgtgtatc cagtatcgaa
     6061 ctgcctcgtg gccactagca gccacgcttt gggcaacttc gcactcgtgg tgggggaata
     6121 gcaggtccat tccaccgcca tgaatgtcga atggttgtcc caggtacttg atgctcatag
     6181 cggagcattc catgtgccat ccgggaaatc cctcccccca gggcgatggc catcgcatga
     6241 tgtgcgccgg atcggcgctt ttccaaagtg caaaatcaaa tgggttctgc ttctcctcct
     6301 gcccatcgag ctgtcgggtg ttggccatca ggtcatcaat cctccgtccc gatagctttc
     6361 cgtaggggta ccgttcgttg taacgggcca catcaaagta aaccgaacca ttgcgaacgt
     6421 aggcgtaacc gttgtcgagt atccttttaa tcagctcaat ttgctcaata atatgccctg
     6481 aggccctggg ttctatgctt ggtggcagca cgttaagctt gcgcatggcg tcgtggtaaa
     6541 ggtcggtgta gcgctgaacc acctccatgg gttccagctg ttccagccgc gccttttttg
     6601 caattttgtc ctcaccctcg tcggcatcat tttccagatg tccaacgtcg gtaatgttac
     6661 gcacgtagcg caccctatac cccaagtggg taaggtagcg gaaaagcaaa tcgaatgtaa
     6721 tggccggtcg ggcgtgccct aaatggggcg caccgtaaac agttggcccg caaacgtata
     6781 atccaacatg gggtggattt atcggtttaa attcctcctt ggttctactt agagtgttgt
     6841 ataggtgcag cttgctatcc ataagtgttt gctgtttgat gaggctgaaa atcttgttga
     6901 tgtatagcgg ctatgggttt actccgcagt aatattttcc cggcaacatc gcaaagcctg
     6961 agccgggttt gtagcatatt ttccttgatg gggtgctgcc tgtttggttg cttcaatttt
     7021 cgctagcatg gctaattcag gaggttttag tgtcatgaaa accatgttac caggtaaaaa
     7081 ttagttagcc gggtaacaat atgcgcaaag gtaaaacttt taggcaaatg ccgaatgggg
     7141 atacgatgac ccggtaattg ttcccccagc caccgaaggt ggttttattt ggagtaacaa
     7201 gaattttttt actgggaagt atcattttcg tggacaacaa tgaatggacg ccgttaacca
     7261 tcgtcggtag ctttacctac ccatggatta gaaagacgag ctgatttgca cgtttttctg
     7321 ctaagggcag ccttttgaga aaatcgaagc agtaatctaa gcaacagacc aacaaggcgc
     7381 ttgtaccaat aactcctgtt gaattgctgg ataaagtttt actatgcaaa aaggtgtggt
     7441 tgcagcaaat ataaccaccg gttactttcc tgtttcctga accttttgta gcaggtagtt
     7501 tttaaattcg tggtttgcct tgtagagtaa aagaatttta cgcggtggaa gtatttcctt
     7561 tagctcctca tttagctcca ccagcaactc ggtttctctt tggcggtttt ccacatacct
     7621 gtcggcgtaa gctaagaggg ttttgtcgtc gggcgactgc tcaataattt tagtgagttc
     7681 accggtaagg gctcggcgct cccttaacac ctcgtttttt ttatcccagt aggcatcgta
     7741 aaccggccag aatttttttg cctcctttgg ggttagctca agcttgctgg tgataaaagc
     7801 taccttctct gccttcagag ctttttcggc atcagcattg gctgtttggg cgcttaccgc
     7861 cgatacggta atcagcaccc aacaggtagc catgaaaatt ttaaggtggc cactcatggt
     7921 ttttcctcgt atttctctcc aataatggtt gtagcgttgc ctttttttat gaagtatcgt
     7981 tgctgaagtg ttctggctgc gctatcaatg ctatcggttt tgcgtttgtt ttcaattgcc
     8041 ttgtacaggt caatatcgtc aaattcgctt atagaacggc tgacgatatt taggtagtcc
     8101 gatttctgaa gggtgtagct atccctggga attgagcgag tagtggtgta gtatccaaaa
     8161 tacgctgcaa caacaagcag ggcaaagcca gcggctaatg ccagctggga tcggagggca
     8221 aaaatccacg attgtttggg ttgttggtgc tggggttggg tgcaccgctc ttgaattcta
     8281 cggggtaagc tgtcgaaata gccatccgga acagagaatg ggagctcttt ccccgccttg
     8341 ccctcaatta cttctaaccc gtttttcttt gcttccatga tgattctaat tcaactgttt
     8401 ctattacgat gatatttgtc agggttggtt taattctccc ccatgaattt ttcaatcttt
     8461 ttcactgcat gatggaatga agctttaagc gccccaaccg aggttccaag aatttcggac
     8521 atttcctcgt acttcatctc ttcaaagtac ttcatgttga aaaccagccg ttgtttctcg
     8581 ggtagtttaa gtatggcctt ttgtagtttt agctgtagct cgttaccatc tatgtaggcg
     8641 tcggtttcga gggttttggc aagttgcgat tctacgtcgt ccatagatag gaatgacttt
     8701 accttttttt tctttagaaa ggtaagcgct tcgtttgtgg caatccggta aagccaggtg
     8761 aagagctgcg attcctccct gaaggtttcg aggccacccc aaaccttgat aaaggtgttc
     8821 tggataacgt cgtcggcatc gtcgtggcca acaacaatct ttcggatatg ccagtagagc
     8881 ctttcctggt acttattcac aataaggtta aaggcgaagt tccgggtttc cccgttgcgg
     8941 aacatttcca gcaactcctt atcggaatag cactccattt caccattagt tattttttcg
     9001 ggcgatagcc ctctttgctg ctttaacaat actgggtgcg tcaaggccgt acttcaccat
     9061 tagctcctcg ggggttccgc tttcgccaaa cgtgtcatta atggcaacca tttcgagggg
     9121 tacgggatga atacgggcaa ccacctgggc aatgctgtcg cccaagccac cattcatcat
     9181 gtgctcctcg gcggtcacgg cacaaccggt tttctgaatt gatctgataa tcgcctcctc
     9241 atccaggggt ttaatggtgt ggatgttaac cacttcggcc gaaattcctt gctgttccag
     9301 catgtcggct gcctcaaggg ctttccatac cagatgtcca gtggcgaaaa cagacacctg
     9361 agttccttcg gttaacttca ccgcttttcc aatggtaaat tgctgatcgg caggggtgaa
     9421 gttaggtact gccggacgtc caaaccgaag gtaaaccggt ccaacgtggt ttgctatggc
     9481 tattgtagct gctttggttt ggttgtagtc gcaggtattg ataacagtca tattgggcaa
     9541 cattttcata agcccaatat cctcgagcat ttgatgggta gccccatctt cacccaaggt
     9601 gattcctgca tgtgaagcgc aaatcttcac atttttattc gagtaggcga cgctttgcct
     9661 aatctggtcg taaacccttc cggtggcaaa aactgcaaag gtcccagcaa agggaatttt
     9721 accaccaatg gtaagacctg cagaaagtcc aatcatgttg gcttcggcta tgccggtttg
     9781 aaaaaatctg tcgggaaatt ctctggaaaa gtcttccatc ttgagcgagc ctatcagatc
     9841 ggcgcaaagg gctacgacat tagggttaac tttacccagt tcatatagac ctgctccaaa
     9901 gcccgaacgt gtatccttgt ttcctgttga agtgtacttc atccgttgta aatgtatttt
     9961 tttaaaacaa aaaatctgtt gaaaattgct gttggtttaa ggtagttctg ttaggagttc
    10021 aggaaagcca acagcagatt accaaattgg caaacgagaa tggtcggtgg ttatttgttt
    10081 cgcttcatac gttcaatggc ctccatgtgg ataagcccaa gaataaccag ctctgcggtt
    10141 gccttatttg ttgccaaagg gatatttttg atgttgcagg cctctagcag taagcgaata
    10201 tcctcatggt gctgctgcac caccttggga tcgcgcagaa agataactat atcaacttcc
    10261 ccgcgttgaa tcatgccggt tatctcgttg taaccacccg attggccagg actaaggtgg
    10321 cggcatttta tcccctgctc ctcaagaaat tcagcagttc tccctgtggc caacaggtta
    10381 actcccggca gccactcttc atggtctttg agaaacctgg ccaactcggg tttcttggca
    10441 tcatgggcaa ttagcgcaat gttcttcatt gtataaatta tttaaatagt ttaaaagtcg
    10501 cctattgttt cgggcagttg tgccatggcc tttgcaaact cctcatcgtt tggggctttt
    10561 ccatgccact tatgattacc caccataaaa tcgacaccca tgcccatctg ggttcgcatg
    10621 agaattatca ccggtttacc cttaccggtg tgctccacgg cttcgtgtag ggttttaact
    10681 agtgcgggca tgtcatttcc attggtttcc agcacatccc aaccaaaggc caaccatttg
    10741 gccttaagat cacccagacc aacaacagtg tcaacagggc catcaatttg ctttccgttg
    10801 tagtcaaccg tggcaataag gttgtctata ccctttgctg cagcaaagag ggcggcctcc
    10861 cagatttgcc cttcctgcag ctccccgtcg ccatgcaggg tgtaaacaag atgactatcg
    10921 ccattcaatt tttttgccag cgcagcacca caacccaccg ataacccctg accaagcgat
    10981 ccggatgcta tccggatgcc gggaagatgt tctgccgggg taggatgccc ctgaagcctt
    11041 gagtttaaga gcctgaaggt tgagagctcc ttaacatcaa agtaccccct acgagccagc
    11101 acgctgtacc atgctgccga aagatgcccg ttggacagga aaaacagatc ttccccggtg
    11161 ccatccatag taaaatgttt aggatcggcc ctaagcacgt cgaagtacag cgctgtgaaa
    11221 aaatcggcac aacccaagga tccacctgga tgtccacttg atggcctgtg caccattcgg
    11281 attacatcgc gccgaacctg agcagccatt ttattaagaa actcgaaatc agtcatctat
    11341 ttgttaaact ttaagaactt tttccaatat acaaatatag taatacttat tgagagttag
    11401 ccgttcaacc ataccggttg ccgtttgtat gtatgctggt gctgccagct aaacgctaac
    11461 cccaaaatca cgtagggcgt tatttaacga agttttgaga tcggttgact ccttacgttt
    11521 accaataatc agggcacagg caacctggta gctaccggca gggaattgct tggtgtagga
    11581 acccggaata acaaccgagc ggggtggaac cattccttta tactccactg gttgggaacc
    11641 cgacacatca attattttgg ttgaccgggt gatggccacg ttggcaccaa gcaccgattc
    11701 cctcccaatg cgaacaccct ccactacaat gcatcgcgaa ccaatgaaac acccatcttc
    11761 aatgataaca ggagccgcct gaacaggttc aagcactccg cctattccca ctccaccact
    11821 caggtgcacg cccttaccaa cctgggcgca tgaaccaacg gttgcccatg tatcaaccat
    11881 cgtcccctca tctacatatg cgccaatgtt gacatacgag ggcatcataa ccacacccct
    11941 ggcaatatag cttccatacc gtgccacggc atggggaact acccttacac ccagcgattg
    12001 gtagttcttt tttaggggta tcttatcgta aaactccagc gggcctgccc ctgtagcctg
    12061 aaagctacat atggaaaagt agagcaaaac ggccttcttc acccattcgt taaccttcca
    12121 cccatcatca gcaggctcgg ccacacgaac ttctcccttg tcaagcattt caaccacgct
    12181 tttaatagca tcgtttacct caggggtacc tagcatttcg cgattttccc atgctgcatc
    12241 tataatttcc tgaagattaa ccataaattg tcagtatttc tgttcaacca attctatatt
    12301 gagaagtcaa aaagtaagta ttttagtaag gttttnnnnn nnnnncagct taaaccttat
    12361 acgggctgca tataatccat aaagtttaaa ctgaaaccaa aacgtggttc agctgcaaat
    12421 gtagcttttt tacaggcaga ttttgataag ggctgattat aacctaaaaa tagaaaatta
    12481 cttggctatg agaaaattga tgctactttc ggttttattg gtagtttcgt taggcttgca
    12541 atcgcaggag ctggttcgct accaagttcc gccggcagaa atggtggcca ttctggaagc
    12601 acctcccact ccgggatttt tgcttagccc cgacggcaaa catgccttgc tgtcataccc
    12661 tcaagttctg cccgacctgg ccgagctggc taagcccgag ctgaagctgg caggcttcag
    12721 ggttaacccc gaaaactttg gacgttacga ttcccgaata ttcgacaggt tggagttgct
    12781 caatattagt aacaaaatgg ttacttctct ttcggggata cccgatagcg gtcatattac
    12841 ctctttcagc tggtcccctg acgggaaaaa tgttgccata agtgtttatt tcaacagctc
    12901 ggttgaactt tggatagttg aggttgaaag tggagtagcc aggccgctat cagctagttt
    12961 gaacgagtct gttatttctc ctggatttgc gtggcatccc gatggcaaat cgctttacgt
    13021 attgaagcac cgtgaaggta tgaaaaagcc cggcagggaa aagttgccct tgggtccatc
    13081 ggtgcagcaa gctacgggac agaagtctca ggtgaggaca tttcaggact tgctcaaatc
    13141 acctgccgac gaggagttgt ttgagtacta cgccacttcg cagctttaca gggtaaattt
    13201 acttggagag gcttccgagg tgggtaaccc gggcataatt tcacaagttg agccatcgcc
    13261 cgatggtagc tatatctacg tggagcaggt tgtaaggcca ttttcataca ttgttccggc
    13321 gcaatatttt ccaaggctct gccatatatg gaacgcggat gggcatttgg taaagcgatt
    13381 agccgaacaa cctcttgccg atgacatacc atcgggattc agcgctgctc gtaaggggaa
    13441 aagggagatg cagtggcgtt cggatgttcc ttcaaccatt gtgtgggtgg aggctcagga
    13501 cgacggcgat ccacaccggg atgttaaaat tcgcgatgta gtttactcgc ttgatgcccc
    13561 gtttagaggc gaaccgttaa aattggttga acttgaaaca cgtttctcct caattagctg
    13621 gggatggagt aaccttgcgg ttgcttacgg ccgttggtgg agtagccgaa ggcaaaccgc
    13681 ttccctattt aatccttcgg caccaaccga taggccaagg gtactttggg ataggtcgtg
    13741 ggaggatgcc tacaccgatc cgggacaact tgtaatggta aataacgcta atgggttcag
    13801 cgtgctacaa accaacagca gcaggacaaa actctacctt agcggtaaag gctcttcgcc
    13861 cgagggcgat atgccctttt tagacgaaat aaacttaaaa aacctaagcg tgaaaaggct
    13921 gtggcaatgc catgccccgc tctacgagta ctttatcggt ttttacgata tggataactc
    13981 gcgtattgtt acctcccagg aatcggttaa caggcagccc aacttcttca tccgtgatat
    14041 caagaggcag aagctggttc agcttaccaa tttccctgac ccatatcctg gcttgcatgg
    14101 agtgcacaag gagctggtta cctaccgacg tcccgatggg gttaccctga ccgggacact
    14161 ctacctgccc gctggctacg aaaagggaac cagaacgcta cccaccctca tgtgggctta
    14221 tcctcaggag ttcctaagtg ccgattttgc cggacaggta accggttcgc cctatcggtt
    14281 tatccggccg tctgttttgt cgcctatact ttgggttgtg cgtgggtatg cggtattcga
    14341 taacatgtca atgccaatag tagccagaaa tggcggtgaa cccaacgata ccttcattga
    14401 acagctggta gagaatgcga aggcagcagt ggatacgctg gtgggcatgg gagtggctaa
    14461 cccccaaaag gtggcaattg gcggccattc ctacggtgct tttatgactg ccaacctgct
    14521 tgcccactcc gatctttttg cagccggaat tgcccgcagc ggggcttaca acagaaccct
    14581 taccccattt ggctttcaga acgaggagcg aacctactgg caagcccctg aggtttacaa
    14641 tcgtatgtct cccttcatgc acgccgataa aattaacgag ccccttttgc tcattcatgg
    14701 cgaaaacgat aataatagcg gaacattccc gctacagagc gaacggctgt ttgccgctat
    14761 aaaaggacac ggtggaacag ccaggctggt aatgcttcca tacgaaagtc atggctacag
    14821 agctaggcag tcggtttttc acaccatttg ggaaatggat tgctggctcg aaaaatacct
    14881 gaaatgatat gagcctgtta atagatttga tgatataatt catggataaa ggattcacgg
    14941 tttatgttta ggagttttct tagcactttt caatttttct tacctgtttg gattttctgg
    15001 aaattcatcc cagtaaattg atggcgttaa aaaaagttaa ccgaagctgc cgcatcactt
    15061 ttaataatta aatattacgc tatttttggg aaaaatgata gcttggttgg ttttcgtaaa
    15121 atatgggtgc ttattttctt tgtggttttc tgcaaagtat cattcagcca ggaaaagcta
    15181 ctaccctttg atacgcttag cattgttcac ttcagggtta tcaatgcaaa cacccgggaa
    15241 cctgttgaaa gagcctacat actgaattta acctacaact ggggaagtat ttccgatttg
    15301 ctgggttaca gctatttgcc gatacacttt ggcgataagc ttaaaatttc tgcaattgga
    15361 tactatactc aagagttgat ttgcgttggc caatttaaag ccgattctgc attttatgag
    15421 ataaagctaa aacccagggt gtatgagttg aacgaggtta aaatcaccaa gttttcgacc
    15481 tacgaaaggt ttcttagagg tgtgattaac ctaaatattc ctaagaccag ggatgataag
    15541 cagctagagg tgattgaaaa atacctgttt ggcgttgtaa agaagatgga tttaaaaaat
    15601 acacccacca tggcttcggg gattgctttt ggtaaggatt ggtatgccaa gcagaatgag
    15661 aagttggcag aacagctgga gagggagaaa acccggaggg taatagatga aaaatttaat
    15721 cccgggcttg tccagaagca tacaggcctg agtgataagg aactttacga gtttatcggt
    15781 tatttagact tcagcgatga gttcctcctg caggctactg attacgagat acatcaaaaa
    15841 ataatggaaa agtataacgt ctttatggcg ggaaaatcaa acgagttaaa aaatcaagaa
    15901 aaatgagtga ggagaattca agctgctatc aaccagaagc ttccgacgta gtttcggcct
    15961 atgagctgat aaaggataag gtgaagcata ctccggttca ttcgtcggcg agtgtaaatg
    16021 ctcttctggg agcaacactc ttttttaagt gtgagaactt tcaaaaagtc ggagcattta
    16081 agtacagggg tgcaacccat gcagttttaa agttgaatga tgaggaggct agaaaagggg
    16141 ttgcaaccca cagttcaggc aaccatgcgg ctgcccttgc gcttgctgct gcaaatagag
    16201 gtattccggc ctatattgtg atgcccgaaa atgcccctgc tattaagaaa aaagcagtag
    16261 ccggttacgg aggtatcatt acctactgcc gatcaacgca gcaggacagg gaggaaacgc
    16321 ttagggttgt tcagcagcaa accggtgcaa ccctcattca cccctacaac aacataaacg
    16381 ttatctgtgg gcagggaaca gctgcgttgg agctgctcga tgaaatgccc gacctggatg
    16441 tggttatagc accggttggt ggtggcggat tgctcagcgg cacagcaacc tttataaagg
    16501 ggcaataccc gcacgtgaaa gtgttcggag ccgaaccaat caatgctgat gatgcctttc
    16561 ggtcgcttcg tgatgggaaa atttaccctt cgctcagtcc atccaccatt gccgatgggc
    16621 tcctcacctc gctttgcccg cttacctact ccattctccg gtcgaagtgc gatggcattt
    16681 ttacggtatc cgagaagggt atagtaaacg ccatgcggtt aatctgggag cgtatgaagc
    16741 ttgtggttga accctcatca gctgttccgg tgggcgttat agctgagcac cccgaggctt
    16801 ttaggggaaa acgagttggt ataattgtct caggtggaaa tgccgatctc gaccatctgc
    16861 cctggctgga tcagtctaat gggtaagggt aaaatttgca caaacagggt agtggtcgga
    16921 aaggttaaga cgcgggcttg aaaattttaa agttttgaat tcaggcgaat gcaggatgta
    16981 gtctatacgg tacgaaggta gaatgccggc ataggtattt agcattccgg cacctgcctc
    17041 tttaaacgaa tcatgcagct tacccgtgag ctccctgtag gtgtatgaaa cgggggtatc
    17101 attaaaatcg ccacaaagaa taaccggata agggcagttt ttcaggtgcc ggttaaccag
    17161 ctcaacctgc tgggaacgct tgatgaaagc gattcttagc ctcgaagcca aatccataat
    17221 ttcgtcatac cggtcattcc cctgccttaa ttcattattt agcagaaagt taaagttgtg
    17281 ctccttaagc ctgagcgatt gcaggtgaag gttgtaaacc ctcacggtat cgccgttgat
    17341 tttaacgtcg gcatagatgg acgagttgag ggtgttctca aatttaattt cacccgaatt
    17401 tactatcgga aatcttgaaa agattgccag accgtatgcc gaacggctat tttgaatgat
    17461 atacccaatc ttggagcgca tgcccagctt ccttttagca tcggcctccg aaaatttagt
    17521 gcttactgtg taaaactcct gaaggcaggc tatatcgaag ttgttgctgc cgatgtagcg
    17581 aatcacccca tcatatgagg gtggaagttt agaccatggg tagagcctga acaggtttac
    17641 attgtatgaa atcaacctga tggaggggac agccgactca tgcttgttgc cagggcgtag
    17701 ctggacaaaa gcgctaaggt gatttattcc tagcagaatg gcgagtagcg aaattaacag
    17761 ctcttttttc catacaatga tccagtaggt caggaaggct atgttggcaa gcaggaaaaa
    17821 tgggtagaat aggcctacaa tggcaggtat ccaaaaccgg gcggggttga tgtaaaccga
    17881 cgcatagcag aatagcagac ctagcgcaaa caaaatgttt gctgcaacaa ttactttatg
    17941 tagaattccg cccttggcca tgctagttca ccgagtcgtt tttcattcga aacagggttt
    18001 ccttttcctc cttgctcagg ctgtcgtagc ctgatttggc tattttgtcg agaatccgat
    18061 cgatagattt ttgctcgttg gcctttcggc ggttgtactc gtagtcggtt tcgggtcggc
    18121 ggtaggaaac cttgagattt ttgctccgat gggctttggg ttttttcacc cggctgaaaa
    18181 tgttgagaaa ttttgtcaaa tcagttcccc tgttaagcag cttgatgaag gagaagccga
    18241 ataccgctcc tcccaggtga gcgatgtgcc caccggggtt tgaaaccgga atgctgatga
    18301 tgtcgaggat tatggtggcc agcgccaggt acttcaactt tacttcaccc agaaagagga
    18361 tgtaaattga attgttgggc ttaagggttg aaaccgctat aacaattgcc aaaaccgatg
    18421 ccgatgctcc aagggccatt gcttcggacg atatagccct gaataccggt agaatattgt
    18481 aggctgcgat aaaaaatatt gctcccgtaa tgcctcccag gaggtaaact cccaccagct
    18541 gcctttgcga gagatgctgt agaaacaacc ttccaaacca gtacaaccac agcaggttga
    18601 atagtatgtg caagaaattt tcgtggaaaa acatgtaggt tagcagcgtc cacggtcgag
    18661 aaagtagctg gtgtaaacct gcgggaacgg caagccagtg ggcgtagctg ctgcccggct
    18721 gtgtgccacc tatgaacatg attaccctaa caaggctata gatcaggaag attgccagat
    18781 taatgtagat cagctgggta aggctgctgc caaacctgaa ggaatctttt atttctttcg
    18841 aaatgctcat gttgtgtagc tggtagtgta aatttataag ttttggtcga aatatccaag
    18901 cagcaacttt tgagagtggt aaaagctgtt agcatgttag ggttgcaaca atggcatatt
    18961 tgctcataag caaatatttc caatttacta aactataagg gtagcccatt ggttgttttc
    19021 tacaattttt caataactag tagaaaacat tttttctcga ttgccagtat tttatcagga
    19081 aaaagccaaa aatcattcca ccaagatggg caaagtgggc aatgttactt ccggcttgcg
    19141 taagtccaag gaacagctca agtccgccgt aaaggataac catccatttg gctttaatgg
    19201 ggatgggggg aaacagaatc atcagaacgg tgttggggaa aagcattccg aatgcaagca
    19261 gcactccata aacagcaccc gatgcgccaa ccgtaggaat attcagctgc agggaaagaa
    19321 gctgttggct tagctgctgg gcctgatcaa tcataatcgg gttgcttgga ttctggaacc
    19381 acttatctag aaagtcggag taaatactgt tgtagaactc gggaaagtgc ttggtaacaa
    19441 acgcggcgaa ggtatcgggc gaaagcgtat tgagcatggc aatagaatct gcctgaatag
    19501 aggagaattg caggtagtta acaagcgagt gaacggcagc agctccaagt ccggtaacca
    19561 tgtagtatag caaaaacctt ttaccgcccc agacctgttc cagtattcta ccgaacatcc
    19621 acagggcaaa catgttgaaa aacaggtggg taaaaccgcc gtgcatgaac atgtgggtaa
    19681 ccacctggta gggtttaaag tgcggcgacc ccggtgtgta aagccccagc atggcattca
    19741 aatcgacccc gtaacgttgc aacatccagg taaccagcca aattacagta ttaatcaaaa
    19801 taagattttt tactaccggg ggtatgctgc ttagaaatcc gcttctactt aaactcattt
    19861 tttttaaaaa tttaaagggt taaaacccgt tacaagttaa acatgaaata atgattaagg
    19921 tttttgagct acttaagcca tttttcaaac tcgctcattt caataagagc aatagttttt
    19981 ttgccgtcgg cggtatagtt tggttccctg caggcaaaga gctggtcaac cacctcctgc
    20041 atttccgatt ccgagagccg cttgccgtag cccactgcag cagctttagc catggataga
    20101 gcaattttct cctttgcgct gtcgaagggt agggcttcat tttcgttaat aattgccagc
    20161 aggttttcga ggaaaagtgc ggggtcggtt atccgcgtgt tcccgggtaa gctgttaatg
    20221 cttatggtgt tatggtttaa attgctaata tccagaccca ggcgtaggaa attttcctgg
    20281 ttagtgatga tagtctcaaa gtcttgcggg tttagctcga tggtttgagg gtaaagctcg
    20341 cgctgggcta catttttttc aaggttaaga tttttgtaga attgctcgta gaggatacgc
    20401 tcgtgtgctc ttttctgatc aaccaccagc atccccgatt taactgcagt gatgatgtac
    20461 tttcccttaa tttgaaagaa cctgccagta ttttcggcaa ctgagtcgtc aaaacccttt
    20521 gaggcgaatg tttggacggc tgtttgtttg ggctcaaagt taaagttggg gttgacgtcc
    20581 tgataaagtt gctcccatcc tttaaccggt tgctgcgggg taaagttgtg ttcccttgtc
    20641 ctctgggcaa acctggctcc ttcatcctca aacgggttaa aattagggtt aagctcaatt
    20701 tcagggagtt caaccggtga atctttgggg aaatggggaa tgctgaaccc agggtcggag
    20761 ctgaagtcaa tggagggggc cacggcaaat ttccccagtg cctctcttac agctgcatgg
    20821 acaatctgcc atactgcacc atcatcctca aacttaacct ccgttttggt agggtgaatg
    20881 tttacatcaa tagaacccgg gtcaaccctg aagtaaataa agtaggttgg aatgctgttt
    20941 gctggtagca ggcggttgta agcatttacc actgccttgt ggaggaaggg acttttcatg
    21001 aacctcccgt tgacaaagaa aaactgttca cccgggctct tccgggcact ttctggctta
    21061 ccaatgtaac cgctaattgc aacaatagag gtttcggcaa cgcaatcaac cagctgcgag
    21121 ctgatggact ttccgaaaag gttcacaatg cgctgcttca ggtttgatgg ggcaagcttg
    21181 taaatctccg agccattatg cgataggctc atggaaatct ccggaaaggc aagggcaaca
    21241 tgctgaaatt cagatatgat atgcttcagc tccaccgagt tgcttttaag gaacttccgg
    21301 cgtgccggta cgttgtagaa aaggttttta acagtcagag tcgaaccaac agggcagctc
    21361 accggttgtt gggcaacgat ttccgatccg cctacctgaa ccattgagcc aacctcgtcg
    21421 gacaacctgc gtgtacggag ttcaacttca gctactgccg caattgaagc cagggcttcg
    21481 cccctgaagc ccattgttcg aatgctgtat aaatcggccg cttccttaat ttttgaagtg
    21541 gcatgacgct cgaagcaaag gcgcgcatcg gtttcggaca tcccaatacc atcgtccacc
    21601 acctgaatcg aagtttttcc accatccttt actatgagct gaatggcctt gcaccccgca
    21661 tcaatggagt tctccaacaa ctccttaact accgaagcgg gacgttgaac cacctcgcct
    21721 gcagcaatct ggttggcaat tgaatcgggt aatagatgta tgatgtctgc catctgggaa
    21781 attaccatta aaaaatgagg tgggctacca gtaaaagaat tgtcaaaact tccagaatat
    21841 tcctggttgg taaatagctg tcggggtgtc ttatccacat cgctcccatg cgctcggggg
    21901 taaatgccga gtttgcccta tcaaccgaac ctcgaactat tcctttgtgg gttttaaccc
    21961 agaaaatggt gtctctgtgc tcttgtgctc caaaaaagaa attatttacc atgccgacct
    22021 gccagcctaa tcaaacctcc aaaattactc attttaattt attgttttga gacggttaga
    22081 tattcacttg ggatttgaaa ggaaactcaa cgagcaaaaa tagcagatag ttgtttacta
    22141 gcctgaaatc tttaaattca cgaagtgtaa ttttggttaa aattttgtag cttacacgta
    22201 gtttaaaaaa tttcgatatt atgaagcccg ctggtgctgt tgagctgatt atcttgactg
    22261 ctgcatccat gcttaacggt tgcacaaaga atgttgagcc gtttcctaca agcgctactc
    22321 ttagcccatc actttcggtt ccgcttgggg aaggcggtgt gtcgcttact aaaacccttc
    22381 aaactcttgg aataccagtg gtcaatcttt cagaagatgt tccacaatgg gctacctatg
    22441 gatttgttta cgttgccgat accattcccc ttaacctgac cgaagtatac aaccgtggcg
    22501 attctattac atacctgatg gttagaacaa atatctggaa tcaatttccg ttgggtggca
    22561 gggcacaggt gtttttcctt gatgctaaca accaagttat cgatcagctt tatgaggata
    22621 tggtgagtgt tggcccagct gagcatggaa gccatggcga ggttgtaaac actgctttcg
    22681 aatcgaacga aaccagcttc aataccagca gaatcagctt attaagcgca gcccaaaagg
    22741 tggtaatata tgccggtttg gaaattacag atgaaggcgt ttcccaatcg aatattacct
    22801 acttcgacca gtaccagctt agggttcagg tcgctttaca ggttgatttt atcatgaacc
    22861 tcaactagca aattatcgtg gcattgcggc gaatagtcaa cctgctgatt tttttctctg
    22921 cctacagcac cattgtttgt gcccagcagg acaacacgct gttcctgctt cataatattc
    22981 cccaatcaaa cttcaccaac ccggcagtgc agattcagtg cccggtgtat gtgggagttc
    23041 ccctgctgtc ctctgttcat ttcaacgtaa attccacggg attctcgtat ggcgatgttg
    23101 ccaatgggtc gggggttgca aacctcaacg ccctggttgc cgggatgcac cggtgggatt
    23161 ttgtttcgga ggaggtgcac ttcactccag tttcgtttgg ttttatgtac gacaggcacc
    23221 ggtacattag ctttgcatgg acggagcggg ttgaaacaaa ggtgtttctc ccaaaaaagt
    23281 tgctttccct ttcggttgat ggaaataccc agtacgttgg cgatgagctg aaaaccggca
    23341 acccaggggc gaatgccgtg tactaccgcg aattttcggt tggttactcc aaaaaggtaa
    23401 attccgggtt gattctgggt gttcatggga aattattgtt tggcctggcc ggtgcgttta
    23461 cccgtcgtag gcctgttaat gttgatgttg atgccattac ccataacatt gatgcccaat
    23521 ggaatccgaa ggtggatgtt gcatacccgc taggtgtggc aaccgatgca agtggtaacg
    23581 ttagcaatgt taccgttggc ggtttttcac ccttgaattt tttcctgaat tttggaaatc
    23641 agggaattgc tgctgatttg ggttttatat ggcagcatga tgatgttaca tggagcggta
    23701 gcttgctgga tttggggttt atttggtggc acaagcagac cacccggttc gagaattcag
    23761 gtcattttgt ttatcgcggc gctacccctg ccgatttgga caatcccgat gactacatca
    23821 accagctggg cgattcccta aacaaccaaa tccaggtgga atactcccgc aaaggattca
    23881 ttacactgct gaacccaaaa atttaccttg gtgcaagcta cccggtatcg gaaaggttta
    23941 atgttggagc ccatgttcgt gcagagtggt accccggtcg ccctgttatg ggtattacct
    24001 tatccggaat gttttttacg aaaaagggtg gttcggcagc cctaagctac tcggtgatga
    24061 atggttcgtt catcaacgtt ggcgctggcc tcggttgggg tggccaaact tttcaattct
    24121 acgtggtgtc cgacaacctt atggctgcgc tctatcccga aaaggcccga aacgccaacc
    24181 ttaggtttgg ttttaacctc tttttcggct gcatcgaaaa aaagaagaag gtggaggtgc
    24241 cccatggcaa tggatgcggc tgctactgga gctgggatga taaaaccagg cgaaaacttg
    24301 gtagaaatta gctgctgtcc ctcttatttg gggttgagcg aaccattttc gtcccggtta
    24361 caagtaaatt gaaatttttg cgggttaata cgatactgca aaagatattt tgcccattgc
    24421 tttcttaaaa actatttgga ctgttaaagc tgaccggcta aaaattgtag ggttctgcct
    24481 tttgtctttt ccacattttc attattttta cttcgttgaa atttttaata taacaagttt
    24541 taaaattttg gttgaaaatc gtaataagct gctgatgaaa aaaatatgcc gaatatttgc
    24601 aattgcagta tggatggccc ctttaggtct tatggcacag ccccctgaag taggtcaggt
    24661 tgccccggat atcctgcttt ccagcccaac tggagattcc ataagacttt cgtcgctaag
    24721 gggcaagctt gtgctggttg atttctgggc atcctggtgt tcaccctgtc gcagggaaaa
    24781 ccccaacctg gtgcaaacct acaacaagtt taaggacgcc tgctttgaga atggtaatgg
    24841 atttaccatt tatagcgttt cgctcgacca gaagcaggat gcatggttga aagccatagc
    24901 cgacgatggc ctggtttggc cctaccaggt tagcgatctt aaaggctggc gtagcgaggc
    24961 cgcaaagcaa tatggcgtga gggcaattcc catgaactac cttatcaacg ccaacggcgt
    25021 tgtggtggct acaaacctca ggggcgacga gctcgacagc accttgcgga agctgcgtaa
    25081 gtggaggatt ttctccaatg agtgtatttg ataatcacat caactagcta acttttatcg
    25141 acatgaaggt tggtcatatt ctgtggaaaa cagctaaaat aattttaatt accctggggt
    25201 cgttaatcct gttggtagtt atactacttg cagcagctaa gctgtcggaa aataagatag
    25261 ttgggctggt gctcaacaag gtaagcgtgg agcttaaggc gccattctcc gtcagggagg
    25321 ttaacctact ccccttacgg aattttccaa acctgactgt tgaacttcag gactttagcg
    25381 ttggtaaatc aaccgattcg cttcaatcag ggcagagcag caaccctttt gatacacttt
    25441 ttagcctgca taagctctac gttgccgtga aagctaaacc acttctgagc agcaaggttg
    25501 tagttgacca ggtggagatt gacggggcaa ccctgaacta tcatgttgac tccaccggtg
    25561 caagcaactt cgatttcctg atgactacca ccgataccac ccaagtacct gaggacacct
    25621 cgcaggttga aaccgttctg aatattctcc tgcacgacct tacactcagc aacattgttc
    25681 ttaactatgc cgacgatagc ctgagggcta aggcttgtgt tgctattcct gaaattaagc
    25741 tcaacggaaa agttatgggc agctactatg caggggcagt agaggggtca atttccgttt
    25801 ctgatttggc tcttgagggt actcatgctg acagaatggg taaaacttct cttgacttca
    25861 aggtagggta cgataatggc gatatttctg ttgataagct caatttgaac gccgatggat
    25921 ttgggctgtc tgcaaatggc aaggctaaac tttccgactc aatttttgta gatatgggtg
    25981 tgggcctcac caatatggat tttggcaggc tgactgccta tctccccgat actcttctct
    26041 ccagctacgg actcactgcc cttggaggca ttttcgacct taaagcccac atcaaaggtt
    26101 ttgtttacga caccctcttg ctcccctcag ttgaggcaag tttggatttc cgaaatggat
    26161 tcatcaaaac cacctcgtac cccactgtcg aaacactagc tttcaacggc aaggttaagc
    26221 tacccgaccc caacaacctt aaaactgcca gcgctgattt tagcacattc agggttaaaa
    26281 cacacgccag caacctgaac ctggctttca agatattgaa tatcgaaaaa cccatctaca
    26341 acgttaaggg cgacatgttc attaccctcg atgaatttgg cagctttatt cccgatagcc
    26401 ttgtcaagag cctttcggga aacatcatgg cccgcatcga aacccgcggg cagctgcccg
    26461 acagcctggg gatgagcagc gccgattact tcatgcaaaa tactactctt aatgttaagc
    26521 ttcgcaacat aaccgctgtg ctagactctt caacctctgt ccgtggtgta aaccttaact
    26581 tcgactttct tcccggaaag caggttgctg tcgaaaattt cacagccgat ttatccattc
    26641 cgggttacca tgtcggcctc gacaattttc tgctacgtgc caggctcatt ggcgatattg
    26701 ctaacaccag caagtttggt gttgatgttg attcactcct cattaaggtt ggaaacagct
    26761 cccttggagg taggttcaag gtaatgaatc tggacaaacc cacctattca gctaaatcgt
    26821 ttgtaaatgt taacttcaac gagctcaggc cttttattcc cgatacgctg gttgaaaccc
    26881 ttacaggacg catggctgca accgtgcagt cgcatggtac cctcaacctc gactcgcttt
    26941 ccagtcaaat tatgcccatt gcttttgagc agagccgctt tacgcttaac ctcgacagct
    27001 tgaactttgc catgcccaac gacaccctga tgagggttga caacctctcg ctgaagcttg
    27061 ccatgagcga cgataccatc cgcattaatg gatttcatgg ccaagctatg ggcattgagt
    27121 ttgccattga ttccacgcga atctggaatg tttacaaggc catgctgaag ggtgagaagg
    27181 ataaaaagtt ggtagccgat acccgaatac agctcagcga tattgatttt gccatgtttg
    27241 ccccattcat ggctaccgat tccactgctg ccgaaccaac acctcaacct gctgaacctc
    27301 aggaaaaagt tgcagggtca gaagcgccgg caaccgaaag cagcttcacc attccatcat
    27361 acataatcag gggcacggtg gctgtcaaca gttttaagta cggcaacatg ctcattgaca
    27421 agatatcaac caaattcagg gttgacgaca gcctttacgt tattgacgac ctgaagctga
    27481 acgcatttgg cggggatctt accctctcgg ctctctacga tacccgtaac gatacggcca
    27541 ccgtaattga ggccaagtgc aacctcaaca gaatgaatat caggcagctt cttgccgata
    27601 acaacgactt tgaccaaacc ttctttaccc atgagaacat cgaaggcctt gccaccggtg
    27661 agttgtttgc ccgggttatc atgcgcgata ccaacatact gtacgacaag ataaacctgc
    27721 tgggcaactt caagctggag aatgggggta tttacaactt cgagcccctc atgcagctgt
    27781 cgaaattcac caacctgaac gagttggaga gggttgtttt cagaacgctc gagaccagcg
    27841 tattcgttta caacaacgag atttacttcc caaaaaccga catcgtaagc tcagccatgg
    27901 acatttcggt ttatggaatg cagagttttg cagatgatta tgagtaccac ctcattgttc
    27961 atcttggtga cgtccttgtt ggtaaatcca acaagctgct caagcagcag ggaatggaga
    28021 gcgacgtgtt tgaaggtcag gacaaggcaa aacgttcggg gctttacctg gtggccctta
    28081 accgggggaa ggattccaag tatggttttg atacgcagca tttgcagcgg ttgatgaaaa
    28141 ccaccatcag ggttcaggag cgtgggctta accttatatt caaccctaag gtggttaact
    28201 ttagcacgga gatagacagg agagaacgca agaagaagcc aaatgaagct aaaggttaag
    28261 ctggacaggc accacctgat tgcctacgcc atatcgcttg ttttagttgt gcttattggg
    28321 tacaagctgt ggcgatattt tacccccgat tccaaagagg ctgtgcttca ggagtcgccc
    28381 gaaccaaggc ctctggatat cgtgtttggc agcgatacag cctcccttcc cgtttacatg
    28441 tatgcaagct atagctgcac cttttgcagg cagtttttta ccgatgtttt tcccatgctg
    28501 aaaaaggaat ttttagacaa cggcagggtt aagctggtta tgaggctgct ggagaatacc
    28561 aaccatcctg acgcgttgaa ttccgcaaaa accgctgtct gcatcaaccg gtatggctac
    28621 ttcgacaagc tgcacgagtt gctccttacc gatagcaggg ttatatacac ccccgaattc
    28681 cgcgacatgg tcaacgactt tacagcaagg gacgagcttg tggccgagtg cattctgggc
    28741 ggacaggccg acgagtacat attgcaaatt aggaacgaat tccgtaaatt tggcctcagc
    28801 ggtacaccca cctttatcat caaccggaat gtttaccggg gttaccgcag ctaccaggag
    28861 ttcaggaaga ttatactaca tcacatggaa acggaggata ggaagggcat aaattaaatg
    28921 atagccactt gtaaacatgg aaatctaagt aaccctacag tgtttaacaa acaaaaaatt
    28981 tatggaaatg aaaaacaaaa tctatttgct gccgttgtcg ttgctggctg tttttacagt
    29041 tgtaacctgg agctgccaga aggatgatga cgaggatgtt tgtcagaagt ttgatcctcc
    29101 acaatgcgaa attgccaatg cttgctgccc taccgatggg ggcaactgct actacgagta
    29161 tggcgatcaa aagttctact gcgacaaaag caaggctaca gccgatgacc ccgatggttg
    29221 caatgcagcc gaagatcagg ttatagatgt aatctgctct accgccaagg gtgctgacat
    29281 ccagctggcc aaggtgcagc tacgggagct aacccgcaag ctgatgaatg aggcccgcat
    29341 gctttcggtt tgcca
//
LOCUS       DAOPHX010000014        29250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_1547_length_29250_cov_4.842679, whole genome shotgun sequence.
ACCESSION   DAOPHX010000014 DAOPHX010000000
VERSION     DAOPHX010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 29250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29250
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1547_length_29250_cov_4.842679"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PK502_02095"
     CDS             <1..746
                     /locus_tag="PK502_02095"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPG99639.1"
                     /translation="NFFIPIPGPKMIWQFGELGYDISIDYNGRLGRKPLHWEYYTQTE
                     RRNLFNIYANLNRLKQEFDVFTTSSYEYMLSGSKKWIKLDGTDMDVVVMGNFDVNGVS
                     FSIDFPATGKWYEYYTGDSIELSTTNATFSMAPSEYRLYTSVKVNRDDIFVGVVENPD
                     ETSERVHLWPNPSNGEFQLSFTLPKALEVQLELYNLMGQKVYHVQHQGILGSNRLKVD
                     LPPEIVTGLYIFRINSQLYNQTGKLSIVK"
     gene            829..1938
                     /locus_tag="PK502_02100"
     CDS             829..1938
                     /locus_tag="PK502_02100"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HPG99640.1"
                     /translation="MVSCKIKTVILLVGMMPLGARVFAQPDSTFDSNARRSALETCLI
                     LGKIIPHDDTLNQLVNRNIKATEIQYSFKKSGRVIPYHQGLSLVIADLGNHDHLGYAF
                     GVIPNVSITTNTKNRVMVNLRLGLGAAWVTKKFSFASNIENVAISTNLNIMASLNANL
                     QIRITEKSKIYLGLNALHLSNGGFKKPNYGLNMLGASGGFSIAFSNKRECKVPKKSSD
                     DLQIETWPQIKLYVGMGVKETGDAGGPKYFPVSLQIAAARRVAENVALGFGVDIAHDR
                     SSKFQIEQSNEVYNSPNDDFQVGASIYAEVPLRRFSVFARQGFYLYNANPDLPIGYQN
                     IGFSYRVGHRVAIFISLKSHLNNADHIYAGLALSI"
     gene            complement(1973..2953)
                     /locus_tag="PK502_02105"
     CDS             complement(1973..2953)
                     /locus_tag="PK502_02105"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPG99641.1"
                     /translation="MDLFGCRRPRLISVQLPVAIIIIIHTLTLQQVVVDLAKRFISKV
                     ISRKSAAINCYFSAVVFALLALFVCHPTFAQVDRCSRDIPHLSANERITYKAYYNWKF
                     IWLRAADILFTSNDTSYNNVPAHHFLAEGRSLKEYDLFFKVRDRFESIVAKEGFRPLY
                     FIRDTYEGGYKAFNKYIYNYTGSSLYMETQTSERPFRIDIKPIKTCTFDVLSAIHYCR
                     GLDFSSHSKGDIIPFAIAIDNEVFELYIRYQGRERLELRDGRIYNTVKFSVKLVEGTV
                     FKGGEDMVVWASDDPGHVPIMVEAKILIGSVKAILSTAENLAFPLLFEKK"
     gene            complement(3131..3907)
                     /locus_tag="PK502_02110"
     CDS             complement(3131..3907)
                     /locus_tag="PK502_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HPG99642.1"
                     /translation="MNYNLPFIPERPAKPRNVGLTMVMDKGLSLSETEGFIESSAEFV
                     DFVKLGFGTSLITNKLKQKIKLYQKGGIRPYLGGTLFEVFIIRGLFNDFVRFVSDLGL
                     DLVEVSDGSMKMDNKVKCDYISKLSEKFTVISEVGSKQADVIIPDDEWVAMMKREVEA
                     GSWKVIAEARESGNVGIYNADHSANVKLIEDITRHLKIENVIWEAPIKSQQAWFIKLL
                     GANVNLGNIAPNESIPLETLRLGLRGDTFFDFLPDNLKQQ"
     gene            complement(4157..5557)
                     /locus_tag="PK502_02115"
     CDS             complement(4157..5557)
                     /locus_tag="PK502_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG99643.1"
                     /translation="MKEDYDSYLDEESEVELVGRFEENLRLNRSVFFDLHEFEGIIDF
                     YLAGDDRAKVRKAIEIAENIYPNSSEIQLKKAEFCFLEERYEDALHLLEFVENVDYHN
                     PELQYLKGEIFYELGDLNKSLQSFDKAIAVATDDRSEMMYRVSSFYLECDEVNLAIKY
                     LLKCHRYDASNVAVLFDLGYCYERNNELDKSISYYNAYLDIYPFSANVWYNMGIIYAK
                     QGKFDESLECYDFCIAVDPSYISAYHNKGNTLSALERYDGAIKVFTELLTLEPDDAGV
                     YSLLGECYERVNNLNMALSCYNKAISIEPDLAEGYFGIGVVMADRQKYDLSLDFIQKA
                     IALDSEQYDFWVGLGKLYYELNRIDEAIKAYREAITINPDEADGYLALAEALLYQEKF
                     SEVEVLVDELGGKFGSNATIKVINAAALYLSHRQKEALTILGDAKRLDPSAIEDFFSL
                     VSIVNDDDFIQKVNLL"
     gene            complement(6030..7316)
                     /locus_tag="PK502_02120"
     CDS             complement(6030..7316)
                     /locus_tag="PK502_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPG99644.1"
                     /translation="MKQQFRRFIHPRYTFLFLLFNVALNVYGQIYTGPIPKPTSGYGA
                     DGGYTVAIQHLSNPGFPDEDIRIYYPSGITSSVPTIFYSHGFGGSDPNNVLGLLSFVA
                     RKGYAIVFVPYQTLGVTTEQRYSTLLDGFIKAVHDFPNIIDTTKVGFMGHSFGGGATF
                     TNAYHCFTSLSWGQSGRFIFASAQYYSFNISQTELQSFPSNTKLLTMVYENDSTNDHR
                     MANDIFNTVNIPITEKDYLMVKSDTIDGYFYEAIHGVPNTLSSFDALDYFAIYRILDA
                     LCDYTFNGSAAGKEVALGNGSVNQVSMPGGMHNLVQTDYPNFAHPDTFYTYPCNLASN
                     PRQEYCNEPTSVGEHRNEKTFKIFPNPSHLSLTISFPSQNFSVEIFNYSGNLIYSKTD
                     IYQAVSIKTANFPNGLYLVRVTDADGNSWIKNFVKE"
     gene            7718..8953
                     /locus_tag="PK502_02125"
     CDS             7718..8953
                     /locus_tag="PK502_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99645.1"
                     /translation="MKRILLFALLLILQINAAISQDAEKHQVEAKVFGFIGYDAILDT
                     YKSLDARDGELIFYPLRPRLDSTGKDINKAVQLQMLSISTRLGLSVKGPDILGAKSSA
                     LVEADFYATKNDYVSLLRIRHAMFKLKWATTELLVGQYWHPVFVTDVAPSVISFGSGA
                     PFHSLNRSPQIRLSYFPYPMVRISLTALMQSYHKSSGPDDAQRNSGKPELMLQAAAGS
                     AEEFLAGLSAGYKWLTPRLVTQTGYATCKTSGQYLFSAFLLKKLGKTVVKAEAVYGQN
                     PTHLNMIGGYGMASGSGPAPNDDYDYTSLNTLSTWVDVQHQAGKMGVGLFAGYSKLYG
                     ADKNYTSLAGYSRNDDLNNIYRISPRLSYQAESITFALEYMLTAATYGSTFDNRHRVT
                     SSLDPVYNHRATLNIKYSF"
     gene            9040..10974
                     /locus_tag="PK502_02130"
     CDS             9040..10974
                     /locus_tag="PK502_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HPG99646.1"
                     /translation="MQRRQRVTAIITATIGLILVLYGLFRNRMLSYVFEQKTERLTTA
                     YNLQVAADRLKFRGIRTLDISNLVVLNSHADTLAQVGAARIKLGLLHLLVLKVNPLAI
                     ELKDSWFNLNTLLERNSIQNKEAITAEANKQEKFNLLPIYKVIKGTFGLSTAKINIDN
                     LTISYIDSLHYETATVTSWNYKNSRFSASVATQHGDRRGSFELAGESSKSRNSFRVWV
                     YGRSFQVEIPFIEALTGVGVYLDSAKLDFRAGELHGNLMALNIETMAKNISMEGSRIA
                     PYPMNIDSASLKLDIKLKPSEICLDSTSTLSLNRLSTSFKSCYTLDKSPTLTLKFKTA
                     TTRWQDLVESLPSGLFTHIAGMKLRGTFSYTLKARVNPESPDSLVIEPLLSSSGFGIS
                     SFGQTNFYALNDTFTHRVYKDGRWIRNIRVSSDNPEFTPLNHISPFLKWAIITSEDGN
                     FYTHGGFDLDGIRYALACNIRERRFARGGSTITQQLIKNLYLNQEKNIARKAEEMLMV
                     WIIENTAAVSKDRMLEIYLNIIEWGPDVYGIAEAAGYYFNKKPNELTLQESLFLAYIV
                     PRPTKFKYLFDDQDRLKPFLYQNFTDVASKMLMRGYITENDFQAIDSTALQLPLHGRA
                     LELIAVEPDLADSTQLVEVE"
     gene            complement(11048..12253)
                     /locus_tag="PK502_02135"
     CDS             complement(11048..12253)
                     /locus_tag="PK502_02135"
                     /inference="COORDINATES: protein motif:HMM:NF040149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99647.1"
                     /translation="MKVATYHLLAAFLLPAFAAGQIAESRVTFDISAAEAFIGTFEAM
                     QQGKSEKQVDSLIDGMLALPAYAVSAERFGAKNQISPVTLGEYKRFLKSFTSDSVDVQ
                     GNTRLGMLKSRYVDTYSNLPDYKKLVNQLKVHLNEYSSGAVQLATEWLPKNVGLNAEV
                     LVMFDIGGAAWAYKASDGKDYIMYSVLFLMDDSRNFDSDMFFKNLAHELHHVGIPLNS
                     FANEVGYDSLPPNDRLKLYIDFMYDIVKEGMAQKFCSNAPGKFTPKPYPNEIYAAIPK
                     GVENWNYFISDFQAINDSVKSFLNFIAYSDSVDAGQFYNRFVRYWTWKAGEVEKKEFA
                     LDRRYYYGAEVLGLVYTTLGKEALFEIMYDYRKLPSYFNLALKKAKPEGYESFLFDDR
                     LADSVLHLK"
     gene            complement(12360..14405)
                     /locus_tag="PK502_02140"
     CDS             complement(12360..14405)
                     /locus_tag="PK502_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPG99648.1"
                     /translation="MNIFVFNITLLEGIAMNKLTLTRRIALLACFAIAANISFAQTSK
                     LAKADALYKAGAYFEAIDLYKADIDKINDKNELPKYLYKLGNCYRLTGNARQAELWYQ
                     KAIMREYPDPKVYFYYAEMLKMGEKYNEAIEQYQKYKVAVPLDPLADAGIKSCELSIK
                     WKETPSGYEITNMRSLNTRTSEFSPAYASSDYATLFFTSSRNEATGGKVSSVTGENFT
                     DIFISERDNKNVWSKPRPIDETINTSYDEGTCALTSDYNTLFFTRCKVSKRDKLGCQI
                     LVSKRAEQGWLTPDELKLAADSMIVAHPSISADGTELYFVSNISGGIGGLDIWKITRS
                     SSTSDDWGEPVNLGGEVNSAGNEMFPYIHPDGTLYFSSDGHPGMGGLDIFRSWVNDEG
                     RREVENMRYPINSSADDFGIVFEKDREAGFFSSRRQGGRGADDIYMFYLPPINFNLIG
                     HVTDEKSKKPVEQATVKLVGNDGSVVTMQTLADGSFRFNLTPNADYVVIASKKGYLNN
                     KAKESTRGLTQSKDFEVSIALTSTEKPIEIPNIFYDYDRWELRPESREALDRLVEILN
                     DNPNIIIELASHTDSRGTKEYNYELSQKRAQSVVNYLIEKGIEPVRLKAKGYAQTQPK
                     VVDDALASRYPFLQVGTVLDQTFIDTLTSDEQKEIVHQINRRTEFRVLKEDYSAKPK"
     gene            complement(14629..16821)
                     /locus_tag="PK502_02145"
     CDS             complement(14629..16821)
                     /locus_tag="PK502_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPG99649.1"
                     /translation="MSKLRFSAFKEAVNRPVVDANGTYSAKQSEIFGINVFDREKMKN
                     FLSAEAYESMITSIDEGKHIDRKIAGQVAAAMKAWAIEHGATHYTHWFHPLNGSTAEK
                     HDAFLSVSYDGKPLEVFNGDMLVQQEPDASSFPNGGIRNTFEARGYTAWDPSSAAFIM
                     EHTLCIPTIFVSYTGEALDYKTPLLKSLALLDRAATSVLEYFDRNAKKVFVTLGWEQE
                     YFLIDEALYNARPDLALCGRTLMGHAAAKDQQLEDHYFGAIPARVSAFMRHFEFEACK
                     LGIPVKTRHNEVAPNQFECAPIFEEANLAVDHNQLIMSLMDRIARQHGFRVLFHEKPF
                     KGINGSGKHSNWSMMTDTGINLLSPGKTPKNNLQFLTFLINTIKAVHDYPDLLIGSIS
                     SESNSHRLGAHEAPPAIMSVFIGETLTRVLDELEERVSDKKMTPDEKTELKLDIIGKI
                     PEILLDNTDRNRTSPFAFTGNRFEFRAVGSSGSCASPLIVLNTAVANQLNQFKKEVDE
                     AIERGIKKDEAIMQSLRRAIIESRKILFEGNGYSNEWVEEAAKRGLPNIPNPIEAFSI
                     FLHNSSQKLLSGNNVLSVKELEARHEIFNETLMKKVQIEARILGDLAINHIIPTAISY
                     QNTLISNVKGLKELYPDEWEKLAVHQIGLIKEISEHISEISNQVNDMVERRKEANRVD
                     DVVERARLYAQNVKPFIESIRVHIDKLEMIVDDEMWPLPKYREMLFIR"
     gene            17061..17552
                     /locus_tag="PK502_02150"
     CDS             17061..17552
                     /locus_tag="PK502_02150"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HPG99650.1"
                     /translation="MGNIRKKYSNDEITVYWQPAECIHSTICFTRLASVFNPRKRPWI
                     DMSGGTTDQILKIVNECPTNALTFSWLENSRNEHETSPKVVREIKPTSEAGTENVKPV
                     KIQVMPNGPLLLSGDFQLFDADGVELKSMKMVSVCRCGHSQGKPFCDGMHFKIGFRDK
                     EEQ"
     gene            17555..17770
                     /locus_tag="PK502_02155"
     CDS             17555..17770
                     /locus_tag="PK502_02155"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HPG99651.1"
                     /translation="MIESAKHAASLHFLKHGPVRVEGTFRIVDIDGNELEVAEAGKVY
                     LCTCGKSNTKPFCDCSHQEELKLRATN"
     gene            complement(17772..18761)
                     /locus_tag="PK502_02160"
     CDS             complement(17772..18761)
                     /locus_tag="PK502_02160"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPG99652.1"
                     /translation="MQKKLNIAIMAGGNSSEENISLMGASQVEGWLDPGKYNPYKVLV
                     KGTDWTCMVGNGVAVPVSKDDFSVSLNGVRVTFDCALIVIHGNPGENGLLQGYFEMLN
                     IPYTTGGVLNEAITFNKHFCKLLLTGTGVKLAREVLVHRGEEIDVDAVASKLGLPVFV
                     KPNASGSSYGVSKVKHPDEILPALNRAFSEDSTVIIEEFIAGRELTCGAFKSFNREFI
                     LPVTEIVPEKEFFDYEAKYLNKSKEITPAPIPEDLYGTIQGLTSKIYDVLECRGIVRV
                     DYIAGANGIYFLEVNTVPGMSEASIVPQQVRVMGENMRDMLTLVIEDALRRAK"
     gene            complement(18882..20252)
                     /locus_tag="PK502_02165"
     CDS             complement(18882..20252)
                     /locus_tag="PK502_02165"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPG99653.1"
                     /translation="MDTIKSYVEANKNRFLEELFELLRIPSISTLPEHKVDMLKAAEY
                     WKKTLIEAGSDRAVVMPTAGNPVVYAEKIINPALPTVLVYAHYDVMPVDPLEQWISDP
                     FEPQIRDGKIWARGANDDKGQSFIHAKAFELMVKTNSLPCNVKFMIEGEEEVGSVSLG
                     KWCADNKELLKSDIILISDTSMMGPDTPSITVGLRGLAYVEVEVAGPSNDLHSGLFGG
                     AVANPAIILAKMIASLHDENNRITIPGFYDDVLEVSAEERAAMARAPFNLQGYKNSLD
                     IDDVHGEVGFSTIERTGIRPSLDVNGMWSGYTGNGAKTVLPSKAFAKISMRLVPNQDY
                     NKVALLFKDYFESLAPKSVKVKVNIHHGGQAYMAPITTPAYLAASLAIEDVYGKKPIP
                     FRSGGSIPIVSTFEQILGVKSLLIGFGLESDAIHSPNENFSLHQFFNGIDTIARFYGY
                     FGGHNV"
     gene            complement(20283..20864)
                     /locus_tag="PK502_02170"
     CDS             complement(20283..20864)
                     /locus_tag="PK502_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HPG99654.1"
                     /translation="MIQIDDKVVSSEILTQRFCCEIASCKGLCCVQGVSGAPLTQQEA
                     DLLDILLPAIKPFLSAKGMQTIEGHGVSVVDLDGDLVTPLVEDKECAFAIFENGAACC
                     AIEKAWRNKSIDFQKPISCHLYPIRVKQYATFTALNYDVWKVCDPARLLGDKLGLPVF
                     RFLRDAIERAYGKEFYNQLEEASKLLSARQPDS"
     gene            complement(20882..22405)
                     /gene="gpmI"
                     /locus_tag="PK502_02175"
     CDS             complement(20882..22405)
                     /gene="gpmI"
                     /locus_tag="PK502_02175"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPG99655.1"
                     /translation="MKHGKVLLMILDGWGIGNKDKSDVIYSTPTPNIDTLTAAYPNAQ
                     LLACGENVGLPDGQMGNSEVGHLNIGAGRIVYQDLVRINKDIKENKLSKMPVLVEALS
                     YAKSNNVSVHFIGLVSDGGVHSLDKHLYNLLDITKEYGLSRVFIHALTDGRDTDPQSG
                     LGFIRNLENHIKTSNGIIASLVGRYYTMDRDKRWERIREGYDLMVHGKGKPTTNMAKA
                     VEESYREGVTDEFIKPIVRVDENGNPVGLIREGDVVICFNFRTDRLREITIALTQKDM
                     PDFGMKTIPLHYVTLTRYDDTFKNVNAIYEKDNLINTLGEVVSVNGLKQLRIAETEKY
                     AHVTFFFSGGREQEFEGESRILIPSPKVATYDLQPEMSAFLVKDAIVEELKKQTHDFI
                     CLNFANGDMVGHTGVYTAIQKAVQTVDTCAGEVVSTARANGYDVIIIADHGNADYALN
                     PDGSPNTAHSLNPVPIIMVSDKFKAVDSGVLADVAPTILTLMGLTIPPEMTGRVLCH"
     gene            22923..25652
                     /locus_tag="PK502_02180"
     CDS             22923..25652
                     /locus_tag="PK502_02180"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HPG99656.1"
                     /translation="MYFANNNGLLRFDGNSWDLFPIPGNSLVRSVLCINDTIYVGAFE
                     EFGYYTYSNGNDLTYTSLTQKIAGKAWGIDEVWRIYKYNNTILFQTFTYIMVLEKGEI
                     SAFPTPEVLQFSFLIGNDFFVQGKNGKIYKFLQNRFEPIDDGGKFAGKQIWAVLKQEN
                     GSTLLATINHGVWEYNNGTVKAWNNAANSYLIKNQIFTASTLRGGYIAFGTIQAGLVV
                     TDEKGEIVLLIDKKRGLQNNTILSLYTDLHNNLWLGLDNGIDYMELNSPISIIDEGLG
                     LFGSCYSTILYNNKLFVGTNQGLYVCDWPIRKNDIGGSFPFQRVEKTNGQVWCLEVID
                     GKLYCAHNFGTFEIDSKTNVEHTSTEEGSWTFLKLKNHPNKMLVGKYKGIHLYVKENG
                     SWRFLKKVKGFDESCRFIAEDENENIWMAHGYKGVYRLVLNEEKDSVKYSYLFDNTKG
                     FPVKTGINMEKLRNQIIFITQQGVFKYNALIDRMEPYSEFNRKLDDTINIRRLTEDSE
                     GNIWILKSNDVKILKQQPDGKTSLEPTPLVLFGNSFVSSYENIYIPDISNVFLGTENG
                     LIHYNPSYPTADSIKFKCYIKSMQTLSRKGEKIFTDYKINRTQPISIKYSNNSIGLTF
                     TSPTYNAGEIAYSYKLEGYDTGWSEWSSNPIKEFTNLPYGNYTFRLKALDAKGRESDE
                     VELKFTISNPWYLSLWAFIAYGITCVLITLILRAAVKAKIRNATTKVKEQKNLEMKVK
                     EEEHKREVLEAEQEIIRLQNENLKVQVEHKNSELASIALQIAHKNEFLNQLKCKLEAL
                     TKNTNPALQKEVLELINKIDFDLRMDDEWKRFEFHFDEVHVGFLKRLKDLYPTLSPNE
                     LRLCAYLRLNMTTKDIAQILNISIRGVEISRYRLRKKLGIEGDTNLIDFMINL"
     gene            complement(25675..27420)
                     /locus_tag="PK502_02185"
     CDS             complement(25675..27420)
                     /locus_tag="PK502_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HPG99657.1"
                     /translation="MVWNDEFSYSGLPDATKWGYDVGGSGWGNNELQYYTENRLENAR
                     VENGNLVVEARKENYGGMEYTSARLVTRQKGDWLYGRMEAYAKLPSGRGTWPAIWMLP
                     TNWAYGSWPKSGEIDIMEYVGYDPGVVHGSIHTEAYNHVIGTQVTSTINVPTAETAYH
                     LYAVEWSPEKIDIYVDNTKYFTFANEHTDYTTWPFDKAFHLLLNIAVGGNWGGAQGVD
                     PNIWPQKMLVDYVRVYQSLSAEQIFITGPGYVAPNQTGVSFTIPNIVNASYIWTIPND
                     ATIVSGQGTNSIVVNWGEQAGEVSVQITHPQAGGTYTYNVKTSTTPSGDRYSLVNFKH
                     DGIEGWSGMLTGPNTINLNLQDSLLRVDYNITDPSAFPYFAYTLPNPVDMSSFTVLNI
                     WMKTWNFSNSVVLRADLFDTDNRLTDVSPVFKFSPVEPGGQFHVYSFDFKNKWGSNSP
                     EYGQVVNQHQVKGVRFYVDYGIYGKVAADSLWLSDIAISTHPLSASYIQQKSQFLSVY
                     PNPCNDYICLYFDSQVVTTYQVSIYTIEGSLAGYFESSQNPCFISTKQLPSGVYVVTT
                     LNSEGFSARVKLVKL"
     gene            complement(27617..>29250)
                     /locus_tag="PK502_02190"
     CDS             complement(27617..>29250)
                     /locus_tag="PK502_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPG99658.1"
                     /translation="SDTSSIAACAKHFVAYGAAQAGRDYNTVDISERTLYEVYLPPFK
                     SALDAGVVTFMSSFNELNGIPVTGNKAMVNDLLKEKWGFNGFVDSDWGSIREMIYHGY
                     AANEYEAGYLAMSSGVDMDLEGRIYLAQLKKLVEDGRITVEQLDEAVRRILRVKFELG
                     LFDNPYKGCDSIREKELLLNPRHLEIAREVTRKSLVLLKNENVLPLSRNIRSIAVVGP
                     LADNQDELIGTWSGRGEGKHVVSVLSGIREKLPNGNIYYAKGCEIVGENKAGFDEAIN
                     AARKADAVVVVVGESAMMSGEALSRAFLDLPGVQKDLVMELNALHKPLVVVVMNGRPL
                     TIEWMDKAVPAILEAWLPGTMGGLAIADVLFGDYNPSGKLPMSFPRNVGQIPIFYYQK
                     STGRPPRQEERYTSKYIDSPVSPLYPFGYGLSYTTFEYSNLSISSPSIKMDETLKVSV
                     DVRNSGKYDGEEVVQLYVRDMVGSVTRPIRELKGFKKIFIKSGESARVEFSLTSNDLA
                     FYNQNMEYIAEPGDFMLFIGGNSDASLGLGFRLEE"
BASE COUNT         8263 a         6771 c         6379 g         7837 t
ORIGIN      
        1 caaacttctt tatccccatc cctggcccca aaatgatatg gcaattcggc gagctaggct
       61 acgatatctc cattgactat aatggtcgct taggccgtaa accactacac tgggagtact
      121 acacccaaac tgagcgacgc aaccttttca acatatacgc caacctcaac aggctaaagc
      181 aggagtttga cgtattcacg accagtagtt acgagtacat gctctccggc tctaaaaaat
      241 ggattaagct ggatggaacc gacatggacg tggtggtaat gggtaacttt gatgtgaatg
      301 gggttagctt ctccattgat ttcccagcga ctggtaagtg gtatgagtac tacacaggcg
      361 actccattga actctccaca accaatgcca cctttagcat ggcaccctcg gaatacaggc
      421 tctacacctc cgtaaaggtg aaccgcgatg acatatttgt tggtgtggta gaaaacccgg
      481 atgaaacctc tgagagggtt catttgtggc ctaatccatc gaatggagaa tttcaactct
      541 cattcaccct accaaaagcg ctagaagttc agctggagct atacaacctg atgggtcaaa
      601 aggtttacca tgtacaacat cagggaatac tgggaagcaa taggttgaag gttgatttgc
      661 cgccagaaat tgttacgggt ctatacatat tccgcatcaa cagccaatta tataaccaaa
      721 caggaaaact ttcaatcgtc aaataggatt ctttttcggt ttaggtttac gaggtgcatc
      781 aaacccgtcc gttgagaaaa attcggacgg gtttactatt tttgaggtat ggtaagctgc
      841 aagataaaaa ccgttattct acttgttggt atgatgcccc ttggcgcaag agtattcgct
      901 cagcccgaca gcactttcga cagcaacgca agaagaagcg ctttggaaac atgcctcata
      961 cttgggaaaa tcattcccca tgacgatacc cttaaccagc tggtaaatag aaatataaaa
     1021 gcaacagaga tacagtatag ctttaaaaag tcaggaaggg taatccccta ccaccaggga
     1081 ttgtcgctgg ttattgccga ccttggtaac catgatcacc tgggctacgc ctttggcgta
     1141 atacccaatg tttccataac cacgaacaca aaaaatcggg tgatggttaa ccttcgtttg
     1201 ggcctgggtg ctgcatgggt aacaaaaaag ttcagctttg catcgaatat tgaaaacgtt
     1261 gccatatcca caaaccttaa cattatggcc agcctgaatg ccaacctgca aatcaggata
     1321 acagaaaaat cgaaaattta tctcggacta aatgctcttc acctgtcaaa tggcgggttc
     1381 aaaaagccaa actacgggtt gaacatgctt ggggcaagcg gtggttttag catagctttc
     1441 agtaacaaga gggagtgtaa agtcccgaaa aaaagttctg atgatttgca aatagaaacc
     1501 tggcctcaaa ttaagcttta tgttggcatg ggagtaaagg aaacaggcga tgccggtgga
     1561 cctaagtact ttcctgtttc gctacaaata gctgcagcac gtagagtagc cgaaaatgtt
     1621 gccctcggtt ttggggttga tatagcacac gaccgctcat cgaagtttca aattgagcaa
     1681 tcgaatgaag tttacaactc gcccaacgat gactttcaag tgggggcaag tatttatgcg
     1741 gaggtgcccc tacgcaggtt ttcggttttt gcccggcagg gtttctacct ctataacgcc
     1801 aaccccgacc ttccaatcgg ctaccaaaat ataggattta gctacagggt tggccataga
     1861 gtagccattt ttatatcgct taaaagccat cttaacaatg ccgaccacat ctatgccggg
     1921 ttagccttaa gcatctgaaa ggtatttgcc caactgtgga ctatgcagca agctattttt
     1981 tttcgaataa caaggggaat gccagatttt cggcagtgct gagtattgct ttaacagaac
     2041 caatcagaat ttttgcttca accatgattg gaacatgtcc agggtcgtcg ctggcccaaa
     2101 ccaccatatc ctcacctcct ttaaaaaccg ttccctccac cagcttaacg ctgaatttca
     2161 ccgtattata aattcttcca tcgcgtagct ccagcctttc ccttccctgg taacgaatat
     2221 atagctcaaa tacctcgttg tcaatagcaa tggcaaaggg tattatgtcg cccttgctat
     2281 ggctcgaaaa atccaagccc ctgcagtagt gaatggcact taaaacgtcg aaggtgcatg
     2341 ttttaatggg tttaatatca atcctgaagg gtcgttcgga tgtctgggtc tccatgtaca
     2401 aggagctgcc ggtatagtta tagatgtact tgttgaaagc tttatagcct ccctcgtagg
     2461 tgtcgcgaat gaagtaaagg ggtcgaaaac cctccttggc cacaatggat tcgaacctgt
     2521 cgcgaacctt gaaaaaaagg tcgtactcct ttaggctccg cccttcggca agaaagtgat
     2581 gtgcaggcac attattatag gaggtatcgt ttgaggtaaa aaggatgtcg gctgctctaa
     2641 gccatataaa cttccagtta tagtaagcct tgtaggtgat gcgctcgttg gcgctgagat
     2701 gtggaatgtc cctggagcat ctatcaacct gggcaaatgt ggggtggcat acaaaaagtg
     2761 caagcaatgc aaacaccact gcactgaaat agcagttgat agccgctgat ttccttgaaa
     2821 ttacttttga gatgaatcgt tttgccaagt caactactac ttgttggagt gttagcgtat
     2881 gaataataat aataatggca actggtagct gaacgctgat tagccttggg cgcctgcagc
     2941 caaataaatc catggaactt cagctgcaaa ttttttaaat caacagacaa aggacaatca
     3001 tttaccctat aacatccacc aggctcccat aattccaatg caggccatac aataagtgca
     3061 tgatcctatc ctttcttgta tgaccttatc attgggatta aatatttacc aaagatctac
     3121 ttaagttggg ctattgctgc ttaaggttat cgggtagaaa gtcgaaaaag gtatcgcctc
     3181 taagcccgag cctaagggtt tcgagtggta ttgactcgtt gggagcaata tttccaaggt
     3241 ttacattagc gcccagaagt ttaataaacc atgcctgctg gcttttaata ggggcttccc
     3301 agataacatt ttcaatttta aggtgcctgg tgatatcctc aataagcttc acgtttgccg
     3361 aatgatcggc attatatatg cccacatttc cactttctcg ggcctcagct attaccttcc
     3421 agctgccggc ttcaacctcc cttttcatca tggctaccca ctcatcatcg ggaatgatta
     3481 catcggcctg cttggacccc acctcggaaa tcacggtaaa tttctcggac agcttggaaa
     3541 tgtagtcgca cttcacctta ttatccatct tcatggagcc atcggaaacc tccaccaggt
     3601 caagacccag gtcgctgaca aagcggacaa agtcattaaa tagcccccgg ataatgaata
     3661 cttcaaaaag agttcctccc aggtagggac gaatgccacc cttctggtat agcttgattt
     3721 tttgcttcag cttattggtg atgagcgagg taccaaatcc cagcttgacg aaatcgacaa
     3781 attcagcgct cgattcaata aagccctcgg tttcggatag gcttaagcct ttgtccataa
     3841 ccattgttaa tcccacattg cgcggcttgg caggccgttc cggaataaag ggaagattgt
     3901 agttcatagt actttatttt ttattagggt ttagattcga aaagttcaaa atttactttc
     3961 cttgcctggc atattcaaac cagcggcctc attaactagt ggcattttca acttccatta
     4021 atcagctatg gaacgcacat agctcttatt atttctttta catccacctt gtgcttttca
     4081 atgctatcca ccgcagcagt tagtatcgca tcattattta aaaagttaac cgggttcatc
     4141 gtagagagct gacaccctaa agcaagttca ccttttgaat aaaatcatca tcgttgacaa
     4201 tagaaaccaa gctaaaaaaa tcctcaattg cagaggggtc tagacgcttt gcatcgccca
     4261 aaatggttaa tgcctccttt tgcctatgcg aaaggtagag agcagcggca ttaatcacct
     4321 taatggtggc atttgagcca aacttgccac ccagctcgtc aaccagcacc tcaacctccg
     4381 agaacttttc ctggtatagc agcgcttcgg ccagtgcaag atagccatca gcctcatcgg
     4441 gattgattgt aattgcctcg cggtatgctt ttattgcctc atcaattcta ttgagttcgt
     4501 agtagagttt tcccagccca acccaaaagt catactgctc ggagtccaaa gcgatagcct
     4561 tctgaatgaa gtcaaggctt aaatcgtact tctggcggtc ggccattaca actccaattc
     4621 caaagtaccc ctcggccaaa tcgggttcaa ttgatatggc cttgttgtag cagcttaaag
     4681 ccatattaag gttgttaacc cgttcgtagc actcacccaa aagggagtaa acaccagcat
     4741 catcgggttc cagggtgagc agctcggtaa aaacctttat cgccccatcg tatcgctcta
     4801 gggccgagag tgtattaccc ttattatggt atgcggaaat gtaggaggga tcaaccgcaa
     4861 tgcagaagtc gtagcattct aaagactcat cgaacttccc ctgtttagcg taaattatgc
     4921 ccatgttgta ccaaacatta gctgagaatg ggtatatatc aaggtaggcg ttgtagtagc
     4981 taatgctttt atccagctcg ttattgcgct cgtagcagta acccaagtcg aagagcactg
     5041 cgacgttgct ggcatcatac ctatggcact tcaagaggta tttgattgca aggtttacct
     5101 catcgcattc taagtaaaaa cttgaaactc gatacatcat ttcggagcgg tcatcggttg
     5161 caacggcaat ggccttgtcg aagctttgta aggatttgtt gaggtcaccc agctcataaa
     5221 atatttcgcc cttgagatac tgcaattcgg ggttatgata atcaacgttc tcaacaaact
     5281 ccagtaaatg aagggcatcc tcgtatcgtt cctcaaggaa acaaaactct gcctttttta
     5341 gctgaatttc cgaagagttg ggatatatat tttcggcaat ttcaatggcc ttcctaacct
     5401 ttgcccgatc gtcgccggca aggtaaaagt ctataatacc ctcaaattca tgtagatcga
     5461 aaaatacaga cctgttaagt cgcaggttct cctcaaaccg gcccaccagc tcaacctcgc
     5521 tctcctcatc caagtagcta tcgtagtcct ctttcattgt ttaaaataac attctaacta
     5581 caaacatagt cattttaacg aaaacttaac taagctccac attaaattta gctaacaatg
     5641 cccctcccat cgtttagttc aacatccaac ttgtgtgggt ataaatatca ccacctaagt
     5701 tggctaaacg atgcatttga agagtggcct tctgcccggt aataaagatg ttacctgctc
     5761 ctgctgactt aaacccacca tttaatgcaa tgcaagcagc tgctgaggcc tacggcaaat
     5821 gcaacattca ggagtaaaac tggattcaag acaatcatta acaaattttg taagggtagc
     5881 aggaaatgcc aggtaaaaaa tttgcataat cacagcaaaa acaaatctaa cggttaactt
     5941 gtagtatgtt attcactagt ccagttaatg ctatgggtac atttggtaga ataaccgggt
     6001 agtcagccat agttttagat tagtaggaat tactccttta caaagttttt tatccacgag
     6061 ttcccatcag catcagtaac cctgaccagg taaagaccat tagggaaatt cgctgttttt
     6121 atactaacgg cctggtaaat gtcggttttt gaatagatta aattacccga atagttaaag
     6181 atttcaacag aaaagttttg tgaaggaaaa gaaatggtca gggataagtg ggaggggttg
     6241 ggaaaaattt taaatgtttt ttcatttcta tgttctccaa cactagtggg ttcattgcag
     6301 tattcctgtc tgggattgct cgccaaatta cagggatagg tataaaaggt atcgggatgt
     6361 gcaaagttag ggtagtcggt ttgcactaaa ttgtgcattc cgcctggcat tgatacctga
     6421 tttacgctac cattacccaa tgctacttct ttaccggcag cacttccatt aaatgtatag
     6481 tcacacaagg catcaagaat acgatatatg gcaaagtagt caagggcatc aaaggaagaa
     6541 agagtatttg gaacgccatg aattgcctca taaaagtatc cgtcaattgt gtcggacttc
     6601 accataagat agtccttttc agtaatcgga atgttgacgg tattaaaaat atcatttgcc
     6661 atgcggtggt cgtttgtgga atcgttctca taaaccatgg tgaggagttt ggtattcgat
     6721 gggaaagact gtaattcggt ctgcgagatg ttgaatgagt aatattgagc agaagcaaaa
     6781 ataaaccgcc ccgattgtcc ccaactcaaa ctagtaaaac aatggtaggc attggtaaag
     6841 gtggcaccac caccaaatga gtgccccata aaaccaactt ttgtggtgtc tataatatta
     6901 ggaaaatcat gtacggcttt aataaaacca tctaaaaggg tactatacct ttgctcagta
     6961 gtcacaccta gcgtttggta gggcacaaaa acaatggcat accctttcct ggctacaaag
     7021 ctaagcaatc ctaaaacatt attagggtca gaacctccaa aaccatgact ataaaatatt
     7081 gtaggcacac ttgaagtgat accagaaggg tagtagattc ttatatcctc atcgggaaaa
     7141 cccgggttag agagatgctg aatcgcaaca gtgtaaccac catcagcgcc atatccgcta
     7201 gtaggtttgg gaataggccc ggtataaatt tgtccatata cattcaaggc cacattaaat
     7261 agtagaaaaa gaaatgtata tctaggatga atgaatcttc taaactgctg tttcataaca
     7321 ttccctatta agagtattct gatattcgta tttcagctgt catctcctgc tattcttaaa
     7381 atcgcgacgg cccgttatta aacaatacaa tctttgtcaa attttcttaa cgttaaacgc
     7441 ttatggggtt aatttgttta ggctgtaata actaaaaatt gaccaactcc acacctagtt
     7501 tacggttcag ccagctcatt ggctagttta tccctgaaaa attaatcaac ccatcatctt
     7561 tttatatttt tataccttat ggcaagcatt ccagttggac tacccatatt tgttgtttgt
     7621 ttagtaaagt ttctacaaat ttgcccagaa atttgagtat tcacctaaga taaaccattt
     7681 ttcagttgaa ctgaaatact aaaattctcc atttcctgtg aaacgaattc tactgtttgc
     7741 ccttctacta attctccaaa taaatgctgc catttctcag gatgctgaga aacatcaggt
     7801 agaggctaaa gtatttgggt ttatcggcta cgatgccatt ctggacacct acaagtcgct
     7861 cgatgcccgc gacggtgagc taatctttta ccccttgcga ccacggctcg acagcaccgg
     7921 gaaggacatc aacaaggcag ttcagcttca gatgctgagc atatccaccc ggctggggct
     7981 ttccgttaag ggaccagata tacttggggc aaaatcgtcc gcgctggtcg aagctgattt
     8041 ctacgctacc aaaaacgact acgtgagcct gttgcgcatc aggcatgcaa tgttcaagct
     8101 aaagtgggct accaccgagc tgctggttgg ccagtactgg cacccggttt ttgttaccga
     8161 tgttgcacca tcagtaattt catttggctc gggagcgccg ttccactcgc tcaaccgttc
     8221 gccccagatt cgcttaagct acttccctta ccctatggtc agaatctcgc ttaccgccct
     8281 catgcaaagc taccacaagt catccggccc cgatgatgcc cagcgtaact ccggcaaacc
     8341 cgagctaatg ctgcaagccg ctgcaggtag tgctgaagaa tttttggccg ggttgtcggc
     8401 cggatataag tggctcaccc ccaggctcgt aacccaaaca ggctatgcca cgtgtaaaac
     8461 atccggccag taccttttta gcgcattttt attgaaaaaa cttggtaaaa ctgttgttaa
     8521 ggcagaagca gtttacgggc agaacccaac ccacctgaat atgattggcg gttacggaat
     8581 ggcttccggt tccggtccag ctcccaacga tgattacgac tacaccagcc ttaacaccct
     8641 tagcacatgg gttgatgttc agcaccaggc ggggaaaatg ggagttggcc tgtttgccgg
     8701 gtactctaaa ctttatggcg ccgacaaaaa ctacacaagc cttgcgggtt actcccgtaa
     8761 cgacgacctg aacaacatct acaggatttc accccgtcta tcataccagg cggaaagtat
     8821 tacctttgcc ctggagtaca tgctgacagc cgctacttat ggtagtacat ttgacaacag
     8881 gcacagggtt acctcatcgc tcgatcccgt ttacaaccac cgtgcaaccc ttaacataaa
     8941 gtattcattc tagcctcccg accagcggca taatgctgcg gacagctacc caaaaccaag
     9001 cagctagctc cggagagcta catcataaaa ttaaacctta tgcagcgcag acaaagggtt
     9061 acagccatca ttacagcaac catagggctg atactggttt tatacggact gttcagaaac
     9121 aggatgttaa gctacgtttt tgagcaaaag actgagaggc taacaacggc atacaaccta
     9181 caagtggcag cagacaggct aaaattcagg ggaatcagaa cgctggatat tagcaacctg
     9241 gttgtgctca acagccatgc ggataccctt gcccaggttg gagctgctcg catcaagctg
     9301 ggtttacttc acctgcttgt attgaaggtt aacccgctgg ccatagagct aaaggattca
     9361 tggtttaacc taaatacgtt gcttgaacga aattccattc aaaataaaga agccattaca
     9421 gcggaagcca acaaacagga gaaatttaac ctgctaccca tttataaagt tatcaagggc
     9481 acatttggcc tatcaacagc taaaatcaac attgataatt tgaccatttc gtacatcgac
     9541 tcgttgcact acgaaacggc tacagtaacc agttggaatt acaaaaacag ccgtttctcc
     9601 gcatcggtag ctacccagca tggcgataga aggggtagct ttgagctggc cggcgaatcg
     9661 agcaagagcc gtaacagctt cagggtatgg gtttatggtc gctcgtttca ggtggaaatc
     9721 ccatttatcg aagcgctcac aggcgtagga gtgtacctgg atagcgcgaa acttgacttt
     9781 cgtgcaggcg agctgcatgg taaccttatg gcattaaaca ttgagaccat ggccaaaaac
     9841 atctcgatgg agggaagcag aatagcccca tacccgatga atatagacag cgcttcgctt
     9901 aagctggaca taaagctcaa gcccagcgaa atctgcctcg attcaacctc aacgttaagc
     9961 ttaaaccgac tttcaaccag cttcaagtcg tgttacaccc tggacaaatc tccaaccctt
    10021 acgttgaagt ttaaaaccgc tactacccgc tggcaggatc tggtggagtc gcttccctca
    10081 ggtttattca cccatattgc cgggatgaaa cttaggggaa cattcagcta cactcttaaa
    10141 gccagggtaa accctgaaag tccagatagc ctggttattg aaccgctgct aagctccagc
    10201 gggtttggaa tcagctcatt tggacaaacc aacttttacg cccttaacga tacctttacc
    10261 catagggttt acaaggatgg cagatggatt cgcaacattc gggttagctc cgataatcct
    10321 gaatttactc cgctaaacca tatttcgcca ttcttgaaat gggctataat caccagtgaa
    10381 gatggcaact tttataccca cggcggattt gacctcgatg gcatacgcta tgcgttggcc
    10441 tgtaatatac gtgaaaggag atttgcacgt ggtgggagca ccattaccca gcagcttatc
    10501 aaaaacctat acctgaacca ggaaaaaaac attgcccgta aggcagagga aatgctaatg
    10561 gtgtggataa tagaaaatac tgcggcggtg agcaaggacc gcatgttgga aatttacctg
    10621 aacatcattg agtggggtcc tgatgtttac ggaattgccg aggcagccgg ctactacttc
    10681 aataaaaagc ccaacgagct tacacttcag gagtcgctat tcctggccta tattgttccc
    10741 cgaccaacaa aattcaagta cctgttcgac gatcaggata ggctgaaacc gttcctgtac
    10801 cagaatttta ccgatgttgc atcgaaaatg ctcatgaggg gttacatcac cgaaaatgat
    10861 tttcaggcca ttgactccac agcgctgcag ctgccgctgc atggaagggc tttagagcta
    10921 atcgcagtag agcccgattt ggccgactca acccagctag tagaggtgga ataaggggaa
    10981 tatgtgggct atcatttagc cagaatacat tctaggtgca ccggatgtaa ttctccatgc
    11041 aaacagttca cttcaggtgc aaaacgctgt cagcaagcct atcgtcaaat aggaagctct
    11101 cgtaaccctc gggtttcgcc tttttcagcg ctaggttgaa gtagcttggt aatttccgat
    11161 agtcatacat gatttcgaac agcgcctcct tccccagggt ggtatagaca agcccaagaa
    11221 cctcggcgcc gtaatagtag cgtctatcca gcgcaaattc ttttttctcc acctcgcctg
    11281 ccttccatgt ccagtagcgg acaaaccggt tgtagaattg tccggcatcc accgaatcgc
    11341 tgtaggctat aaagtttaaa aatgacttaa ccgagtcgtt aatggcctga aaatcggaga
    11401 taaagtagtt ccagttttca accccttttg gaatagcggc ataaatctcg ttagggtatg
    11461 gttttggtgt aaactttcca ggcgcattgc tgcagaattt ttgggccata ccctccttaa
    11521 caatgtcata cataaaatca atgtagagct taagcctgtc atttggggga aggctgtcgt
    11581 agcccacctc attagcaaaa ctgttgagcg ggattccaac gtggtgcagc tcgtgggcca
    11641 gatttttaaa gaacatatcg ctgtcgaaat tcctgctgtc gtccatcaga aacagaacgc
    11701 tatacatgat ataatccttc ccgtcggagg ctttgtatgc ccaggcagct ccaccaatgt
    11761 cgaacattac aagcacctcc gcattcaacc ccacattttt aggaagccat tcggtggcaa
    11821 gctgcacagc tccgctactg tactcgttca gatgaacttt gagctggttc acaagctttt
    11881 tgtaatccgg gaggttacta taggtatcaa cataccggct ttttagcatt cccaatctgg
    11941 tatttccctg aacatcaacc gaatcggagg taaaactttt caagaaacgc ttatactctc
    12001 cgagcgtaac cggcgaaatt tgatttttcg caccaaatcg ctcggcacta acagcgtatg
    12061 caggtagggc aagcatccca tcaataaggc tgtcaacctg tttttctgat tttccctgct
    12121 gcatggcttc aaaagtccca ataaaagcct cagcagccga aatgtcgaaa gtaacccttg
    12181 actctgcaat ttgcccagct gcaaaagccg gtagcaaaaa agctgcaagc aggtggtaag
    12241 tagctacttt caactttatt gcggttaaac ggttaccttt catccttaat aaatcatcaa
    12301 ttgaactcca tttataagtt ttcgaccaac aatttctatg cttacaggga ttgttgtagc
    12361 tacttaggtt ttgccgagta atcctcctta agcaccctga actcggttcg acggttaatc
    12421 tgatgaacaa tttccttttg ctcgtccgag gtgagcgtat cgataaacgt ctggtctaaa
    12481 actgttccaa cctgaaggaa ggggtacctg gaagccaggg catcgtcaac aactttaggt
    12541 tgggtttggg catatccctt ggccttcagc ctaactggct ctataccttt ctcaatcagg
    12601 tagttcacta ccgattgggc gcgtttctgc gatagctcgt agttgtactc cttggttccc
    12661 cggctatcgg tgtgcgaggc cagctctatg atgatgttgg ggttatcgtt caggatttcc
    12721 acgagtcggt caagcgcctc acgcgattcg ggccgaagtt cccacctatc gtaatcgtag
    12781 aaaatattag gtatctcgat cggtttttcg gtggaggtta gggcaatgga aacctcaaag
    12841 tccttgctct gggtaagccc tctggtcgat tcctttgcct tgttattgag gtagcccttc
    12901 ttggaggcaa tcaccacgta gtcggcgtta ggagtgaggt tgaacctgaa agagccatcg
    12961 gccagggttt gcatggtaac cacggaacca tcgtttccaa ccagcttaac ggtagcctgt
    13021 tcaacgggct ttttgctttt ctcatcggtt acatgcccaa tcaggttgaa gttgattggg
    13081 ggaaggtaaa acatgtagat atcgtcggcg cctctgccac cctgacggcg tgaggagaaa
    13141 aaaccagcct cgcgatcctt ttcaaacaca attccgaaat cgtcggcgga ggagttaatg
    13201 gggtagcgca tgttttccac ctccctccta ccctcatcgt tcacccacga cctgaagatg
    13261 tcaagaccgc ccattccggg gtgcccgtcg gacgaaaagt agagcgttcc atcggggtga
    13321 atgtagggga acatctcgtt gcccgcagaa tttacctcgc ctccaaggtt aacgggttca
    13381 ccccaatcgt cggaggttga gctgcttcgg gtaatcttcc aaatatctag ccccccaatg
    13441 cctccggaga tgttgctaac gaagtacagc tcggtgccat cggccgaaat tgaaggatgg
    13501 gcaacaatca tgctgtcggc ggcaagtttc agctcatcgg gtgtaagcca gccctgctcg
    13561 gctcgtttgg aaaccaatat ctggcatccc agcttatcgc gcttgctcac cttgcaacgg
    13621 gtaaagaaaa gggtgttgta atcggaggtt agggcgcagg ttccctcatc ataggaagta
    13681 tttatggttt catcaatagg gcgtggctta ctccaaacat tcttattatc acgttcgcta
    13741 ataaaaatat cggtaaagtt ctctcccgtt accgagctaa ccttgccgcc tgttgcctca
    13801 ttgcgtgagc tggtgaaaaa aagcgtggca tagtcggacg aagcataagc agggctaaac
    13861 tcactggtac gggtgttaag gctacgcatg ttggtaattt cataccccga gggtgtttcc
    13921 ttccacttaa tggatagctc acatgatttt ataccggcat cagccaaggg gtcaagcgga
    13981 acagccacct tgtacttttg gtactgctca atggcctcgt tgtacttttc gcccatttta
    14041 agcatttcag catagtagaa gtaaacctta gggtcgggat actcacgcat aatggctttt
    14101 tgataccaga gttcggcctg tcgggcgtta ccggtgagcc ggtagcagtt gcccagcttg
    14161 taaagatact ttggtaactc attcttatcg ttaatcttat cgatatcagc cttgtacaga
    14221 tctattgctt caaaataggc tccggcctta tacagggcat cggctttagc cagcttgctg
    14281 gtttgggcaa acgatatatt tgcagctata gcaaaacatg ccaaaagggc tatccgtcgg
    14341 gttaaggtta atttattcat ggctattcct tcaagtaggg taatgttgaa tacaaaaata
    14401 ttcaaaatat ttattccacc atgaattagc tcaccacgat ttcaaatatc aacggaacag
    14461 gaaaaagtta tgatagggtg ataaaaaata gaacgaagct gttggttaac tgcttcgttc
    14521 tataatggat tattgcgacg tttaatgtaa tgcaggagct atgcaaacct acaagaaatt
    14581 gcaaaattta accaaaacat ccaaaaccaa aaccacaggg cgtttcaatt accttataaa
    14641 gagcatttcc ctgtacttgg gcagaggcca catctcgtca tccacaatca tctccagctt
    14701 atcaatgtga acgcgtatgc tttcgatgaa gggcttgacg ttttgagcgt ataaacgagc
    14761 ccgttcaacc acatcgtcaa cgcggttagc ctccttgcga cgttcaacca tatcgttaac
    14821 ctggttgctt atctcgctaa tatgctcgga gatttcctta ataaggccta tttggtgcac
    14881 ggccagcttc tcccattcgt cggggtaaag ttcttttagc ccttttacat tgctaatcag
    14941 cgtgttctga taagaaatag ctgttgggat gatatggtta atggccaaat cgcccagaat
    15001 acgggcttca atttgcacct tcttcatcag ggtttcgtta aaaatctcat gccgggcttc
    15061 cagctccttc acagaaagga cattgttgcc ggagagaagc ttttgggacg aattgtgaag
    15121 aaatatactg aaggcctcaa ttgggttggg aatgttgggc aaccctcgtt tagcagcctc
    15181 ttcgacccat tcattgctat agccgttgcc ctcgaagaga atttttcgag actcgataat
    15241 tgcccttcgc aggctctgca taatggcctc atcctttttt atgcctcgct caatcgcctc
    15301 gtcaacctcc tttttaaact ggttaagctg gttggcaacg gcagtattca acacaatcag
    15361 gggcgatgcg cagcttccgg aggaacccac agcacggaat tcgaagcggt tgccggtaaa
    15421 ggcaaaaggt gaggtgcggt tgcggtcggt gttgtcgagc agaatttccg gaattttccc
    15481 tataatgtcg agtttaagct ccgttttttc atcaggggtc atttttttat cgctaacccg
    15541 ctcctccagc tcgtcgagca cgcgggtaag cgtttcgcca ataaaaaccg acataatggc
    15601 gggtggagct tcgtgggcgc ccagcctatg cgagttcgac tccgacgaaa tgcttccaat
    15661 cagtagatca gggtagtcgt ggactgcttt tatggtgtta ataagaaaag tgaggaactg
    15721 taggttgttt ttcggtgttt taccgggcga tagcaggtta atccccgtgt cggtcatcat
    15781 ggaccagttg ctatgcttgc ctgatccgtt aataccctta aaaggttttt catggaaaag
    15841 tactctaaat ccgtgttggc gggcaattct gtccataagc gacatgatga gctggttgtg
    15901 gtccacagcc aggttggcct cctcgaaaat tggggcgcat tcaaactggt tgggtgccac
    15961 ttcattgtga cgggttttca ccgggattcc aagcttacag gcttcaaatt cgaaatggcg
    16021 cataaaagca gatacccgtg ccggaatggc gccaaagtag tgatcctcca gctgctgatc
    16081 ctttgctgca gcatggccca tcagggttct tccgcaaaga gcaagatcgg gacgggcatt
    16141 gtagagcgcc tcgtcaatca agaagtattc ctgctcccac cccagggtaa cgaaaacctt
    16201 tttagcattc cggtcgaagt actccagaac ggaggtagct gccctatcga gtagggccag
    16261 cgacttcagc aggggtgttt tgtaatccag tgcctctcct gtgtaggata caaatatggt
    16321 aggtatgcaa agcgtatgct ccatgatgaa cgctgcagaa gaagggtccc atgccgtgta
    16381 gcccctggct tcaaaggtgt ttctaattcc tccgttcgga aagctggacg catcgggctc
    16441 ctgctgcacc agcatgtcac cattgaatac ttcaagtggc ttcccgtcgt agcttaccga
    16501 aagaaaagca tcgtgctttt cggctgtaga gccattcagc gggtgaaacc agtgggtgta
    16561 gtgtgttgca ccatgctcta tagcccatgc cttcatggct gcagccacct gccctgcaat
    16621 tttccggtct atatgcttgc cctcatctat tgaggtaatc atgctctcat aggcttctgc
    16681 cgacaggaaa tttttcatct tttccctgtc gaagacattt attccgaaaa tttcagactg
    16741 cttggcacta tacgtaccgt tagcatcaac taccggacga tttactgcct ctttgaatgc
    16801 cgagaatcgt aattttgaca tgagatttta gtttgatgct gcaaatatat acttatttta
    16861 acacacaccc tcaaatacta atatcattta ttaaattatt tgtataaata tttttattta
    16921 cccatttttt tttaagggta taagtgtaaa attcgtaatt tgtgtttcta tggcctttat
    16981 actaaaaaat tttaatttag cagctgaaca tttacagaat tcgctacgtt tgtaaaaaaa
    17041 tacccttcag ccacttcatc atgggaaaca tcaggaagaa gtatagcaac gatgagataa
    17101 ctgtttactg gcaaccggca gagtgcatac attcaacaat ttgtttcacg aggctggcat
    17161 cggttttcaa tccccgtaaa cggccatgga ttgacatgag cgggggaacc accgatcaga
    17221 tacttaaaat tgtaaatgag tgtccgacaa acgccctcac cttcagctgg ctcgaaaaca
    17281 gcagaaacga gcatgaaacc tctcccaagg tggtcaggga gattaagcca acaagtgaag
    17341 caggcactga gaatgttaaa ccggtgaaaa ttcaggtaat gcccaatggg ccgttgcttc
    17401 tatccggaga ttttcagcta ttcgatgctg atggtgtcga gctgaaaagc atgaaaatgg
    17461 tatcggtgtg ccgatgtggg cactcgcagg gaaagccatt ctgcgacggc atgcatttca
    17521 aaattggatt tagggataag gaggagcaat aacaatgatc gaatcggcaa aacatgcagc
    17581 atcgctgcat ttcttaaagc atggcccggt gagggttgag gggacattca ggatagttga
    17641 cattgacgga aacgagctgg aggttgccga agccggtaaa gtttaccttt gcacctgcgg
    17701 aaaatcgaac accaagccct tttgcgattg cagccatcag gaagaactta agctaagagc
    17761 tactaactaa actattttgc ccttcgcagg gcatcctcga taaccagcgt tagcatatcg
    17821 cgcatatttt cacccattac cctcacctgc tggggcacga tgctagcttc gctcataccc
    17881 gggacggtgt ttacctccag aaagtaaatg ccatttgctc ccgcaatata gtcaaccctg
    17941 acaattcccc gacattccaa aacgtcatat attttagagg ttagcccctg tatggttcca
    18001 tacaggtctt caggaatagg agcgggagta atctctttgg atttgttaag atacttggct
    18061 tcatagtcga aaaactcctt ctcgggaaca atctcggtaa cgggcagtat gaactccctg
    18121 ttaaacgact taaaagcacc acaggtcagc tccctaccgg caataaactc ctcaataatg
    18181 acagtgctat cctcggaaaa tgccctattg agagcaggca gaatttcgtc cgggtgcttc
    18241 accttgctaa ccccgtagct gctaccgctg gcgttgggct tcacaaaaac aggcaacccg
    18301 agtttactag ctacagcatc aacatcaatt tcttctcccc gatgaaccaa aacctctctt
    18361 gccagcttta cccctgtacc tgtgaggagc aacttgcaga agtgcttgtt gaaggtaata
    18421 gcctcgttga gcacaccacc cgttgtgtag ggtatgttga gcatttcaaa gtatccctga
    18481 agtaagccgt tctccccggg gtttccatga ataacaatta gtgcacagtc aaaagttacc
    18541 ctaacgccat tcaatgaaac actaaaatca tccttcgaaa ccggaacagc aacaccatta
    18601 ccaaccatgc atgtccaatc ggttccctta accagaacct tataaggatt gtactttcca
    18661 gggtcgagcc aaccctccac ctgcgaggcg cccataagtg aaatattttc ctctgatgag
    18721 ttgcccccag ccataatggc tatattcaat tttttttgca tggcagtaaa attaagctgc
    18781 aaagctgcaa gaaaatttgc aaaaattggt gttccccttt tgaaaatttt tctaccctaa
    18841 atatgaaact gtttgctagt gcaggtatca gcagcgatta gctaaacgtt atgcccacca
    18901 aagtagccgt aaaagcgggc aatcgtatcg ataccattga aaaactggtg gagcgaaaaa
    18961 ttttcgttag gggaatgaat ggcatccgat tcaagaccaa agccgatgag tagggatttt
    19021 acgcccaaaa tctgttcaaa agtgctgaca attggaatac ttcccccact tctgaaagga
    19081 atgggctttt tgccatatac atcctcaatt gccaggctgg cagcaaggta agcgggtgtt
    19141 gtgattggag ccatataggc ctgtccacca tgatggatat ttactttcac cttaacggat
    19201 ttgggcgcca gggattcaaa gtaatcctta aaaagcaagg caactttatt gtagtcctgg
    19261 ttagggacaa gacgcataga aattttagca aaagcttttg atggcagaac ggttttagcg
    19321 ccattgcccg tatagccgct ccacataccg ttcacgtcta atgaggggcg aattcctgtt
    19381 cgttcaatgg tggaaaatcc aacttcgcca tgaacatcgt caatgtcaag tgagttttta
    19441 tagccctgaa ggttgaaggg agccctggcc attgctgccc tctcttctgc gcttacctcc
    19501 agcacatcat cgtaaaaacc gggtattgta attcggttgt tttcgtcatg gagcgaagca
    19561 atcattttgg ccaaaataat ggcagggtta gctactgctc caccaaaaag tccggaatgc
    19621 aagtcgttac tgggtccggc tacctcaacc tcaacataag ctaagcccct tagtccaacg
    19681 gtgattgagg gggtatcggg acccatcatt gaggtgtctg aaattaatat gatatcggat
    19741 ttaagcagct ccttgttatc ggcgcaccac ttcccgaggc taaccgagcc aacctcctcc
    19801 tccccttcga tcataaattt cacattgcag ggaagggagt tggtttttac cataagttca
    19861 aaagctttgg catgaatgaa cgattgtccc ttgtcgtcgt tggcaccccg tgcccagatt
    19921 ttaccatcac gaatctgggg ttcaaaaggg tctgaaatcc attgctctaa cgggtcaacg
    19981 ggcattacgt cgtagtgggc gtaaaccaaa actgtgggaa gagctgggtt aataattttt
    20041 tcagcataaa ccacaggatt gcctgcggta ggcataacca cagccctatc agaaccagct
    20101 tctataagtg tttttttcca gtactcagca gctttaagca tgtcgacttt atgctccggc
    20161 agggtgctaa tggatggaat gcgaagaagc tcgaacagtt cttccaaaaa gcgattttta
    20221 ttggcttcaa catacgactt tatggtatcc atggcaggta aaagtattgg tttttaataa
    20281 ttttagctgt cgggttgccg ggcagaaagc agcttgcttg cttcttcaag ctggttgtag
    20341 aactcctttc cgtaggcacg ctcaatagca tctcttaaaa aacggaaaac aggcagccct
    20401 aacttatcgc ccaacaagcg tgcggggtca cataccttcc agacatcgta gttaagtgcg
    20461 gtaaacgtgg cgtactgttt taccctgatt gggtacaaat ggcacgaaat aggcttctga
    20521 aaatcaatgc ttttattcct ccatgccttc tcaatggcac aacatgcagc tccattttca
    20581 aaaatagcaa aggcgcattc cttgtcttca actagaggag taaccaggtc gccatccaaa
    20641 tcaaccacag agactccatg cccttcaatg gtttgcatgc cctttgctga aagaaatggc
    20701 ttaatggctg ggagcaaaat gtcaagtaag tcagcctcct gttgggtaag aggtgcgccc
    20761 gatacccctt ggacacagca taaacccttg caggaagcaa tttcgcagca aaacctttgg
    20821 gtaagtattt cgctgctgac caccttgtcg tcaatctgaa tcatcatgtt ggcggggtgt
    20881 gctagtggca caaaaccctt ccggtcattt ccggaggaat agtcagcccc atcagggtaa
    20941 gaatggtggg tgccacatcg gccaggacac cgctatcaac agctttgaac ttatcggaaa
    21001 ccatgataat ggggacggga ttaagcgaat gggctgtgtt tggcgaacca tcgggattaa
    21061 gagcatagtc ggcgttacca tggtcggcaa taataattac gtcatatccg ttagccctgg
    21121 ccgtgctaac aacctccccg gcacaagtat caaccgtttg aacggccttt tggatggccg
    21181 tgtaaacgcc agtgtgcccc accatgtcgc cattagcaaa gttaaggcaa ataaagtcat
    21241 gggtttgctt tttaagctcc tccacaatgg catccttaac aagaaacgca ctcatttcgg
    21301 gttgcaaatc gtaggtagcc accttgggcg aagggataag tattcggctt tcgccttcaa
    21361 actcctgctc cctcccaccc gagaaaaaga aggtaacgtg ggcatatttt tcggtttcag
    21421 caatgcgtag ctgcttcagg ccattaacag aaacaacctc gcccaacgtg ttgatgaggt
    21481 tatccttctc gtaaatagcg ttcacatttt taaaagtgtc gtcgtagcgg gtaagggtaa
    21541 cgtagtgaag cggaatggtt ttcattccga aatcgggcat atccttttga gtaagggcaa
    21601 tagtaatctc gcgcagacgg tcggtgcgga agttaaagca gataactaca tcgccttcgc
    21661 gaataaggcc gacagggttt ccgttctcgt caaccctgac aatgggcttg ataaactcat
    21721 cggttacgcc ctcccggtat gattcttcaa cagccttagc catgttggtc gtgggcttgc
    21781 cctttccatg aaccatcagg tcgtaaccct cgcgaatgcg ttcccaacgc ttgtccctgt
    21841 ccatggtgta gtagcgacca accagcgagg cgataatccc attgctagtt tttatatggt
    21901 tctcgagatt tctgataaag cccagaccgc tttgtgggtc tgtatcgcgg ccatcggtca
    21961 gagcatgaat gaatactctt gacaggccat actccttagt aatatcaagc aggttataca
    22021 aatgcttatc gagtgagtga actccgccat cggaaaccag accaataaag tgaacgctaa
    22081 cattattact cttggcgtag ctaagagcct caaccagcac gggcattttg ctcagcttgt
    22141 tttccttaat atccttgttt atccgcacca gatcctggta aactattcta ccagcgccaa
    22201 tgttcaggtg tccaacctcc gaatttccca tttggccatc aggtaggcca acgttttcac
    22261 cacatgccag gagctgggcg ttggggtaag ctgccgttag ggtatctata tttggcgttg
    22321 gggtgctgta tattacgtcc gatttatcct tatttccaat accccagcca tcaagaatca
    22381 tcagtaaaac ctttccgtgt ttcatatcct gtaagttaaa ttttcattta aaaccacaca
    22441 ccaggaacgt tttcagtaac ctttatgttc gtacacgact acaaagttac ccttttttaa
    22501 ttgccttcgg ctaatgttgg cttattattg accggttgtg gcgtaaacgt ttgaaaattg
    22561 tactgacacc tatcagctta ccctgctatt tgttgaaaaa ttaaccgttc gtagtaattt
    22621 tttaataatt ttatcccaaa tgatgaaagg ttgttattgc cagatttaaa aatcgaacgg
    22681 agcatataaa ccaaagtcca ttagaaacat agcccataaa aatgaacttt tctaggagta
    22741 ttaaatcaac cagcataacc aaaaggagca tccctctgct taccacatta ttaatattca
    22801 cttttactct aggccattca cagataatta ccacaggatt gcctgtaatg aagcatttcc
    22861 gaaaaactga ctacagggca ggcacgcaaa actggggtat ttcgcaggat aaacaaggtt
    22921 ttatgtattt tgccaataac aatggcctgc tccggtttga cgggaatagc tgggatcttt
    22981 tccccatccc cggaaacagc ctggttagat cggtgctgtg tataaacgac accatttatg
    23041 taggggcatt cgaggaattt ggctactaca cctacagtaa tggcaacgac cttacctata
    23101 cttcgctcac ccaaaaaatt gcgggaaagg cgtggggaat agatgaagtt tggagaattt
    23161 acaagtacaa caacaccatt ctttttcaaa catttaccta tatcatggta ctcgaaaagg
    23221 gtgagataag cgcttttccc actcccgaag tgctccagtt ttcattcctc atcgggaacg
    23281 atttttttgt tcaggggaag aatggtaaaa tctataaatt cctgcaaaat agatttgagc
    23341 caatagacga tggtggaaaa tttgccggaa agcagatttg ggcagtacta aaacaagaaa
    23401 atggttcaac cttgctggca acaataaacc acggcgtatg ggagtataac aatgggactg
    23461 ttaaggcatg gaacaatgct gcaaatagct atttaattaa aaatcaaatt ttcacagctt
    23521 caacgctgcg cgggggatac atagcttttg gaaccataca ggcgggtttg gtggtaaccg
    23581 acgaaaaggg agagattgtt ctactaattg acaagaagag agggcttcaa aataacacca
    23641 ttttaagtct ttacaccgac ctgcataaca acttatggct aggactagat aatggcattg
    23701 actacatgga gctgaactcg cccatatcta ttattgacga aggtttgggg ttgtttggtt
    23761 cttgctactc aacaatactg tataataata agctgtttgt aggaaccaac caggggctct
    23821 atgtttgcga ctggccaatt aggaaaaatg atataggtgg aagttttcct tttcaaagag
    23881 tagagaaaac taacggacaa gtgtggtgtc ttgaggtgat tgatgggaaa ctatactgtg
    23941 cccataactt tgggactttc gaaattgatt ccaaaacaaa tgtagagcat acttccaccg
    24001 aagaggggtc gtggaccttc ctgaagctaa aaaatcatcc gaataaaatg ctggttggaa
    24061 agtacaaggg aatacatcta tatgtaaagg agaatggttc gtggcgcttt cttaaaaagg
    24121 ttaaaggttt tgatgagtca tgccgtttta ttgctgagga tgaaaatgaa aatatatgga
    24181 tggctcatgg ttacaagggt gtgtatcgac ttgtgctgaa tgaagaaaag gatagcgtaa
    24241 aatactccta ccttttcgat aacaccaaag ggtttccggt taaaacaggg attaacatgg
    24301 aaaagcttcg aaaccaaata atcttcatta cccaacaggg agtattcaag tataacgcac
    24361 taatagacag gatggagcca tattccgaat ttaaccggaa actggacgat acaattaaca
    24421 taagacgatt aaccgaggat agcgagggca atatatggat actaaaatca aacgatgtaa
    24481 aaatcctaaa acagcaaccg gatggcaaga catccctcga gcctacccct cttgtacttt
    24541 ttggcaattc ctttgttagc tcttacgaaa atatttacat tcctgatatt tcaaatgttt
    24601 tccttggaac tgaaaatgga ttaattcact ataacccaag ctaccccact gccgacagca
    24661 ttaaatttaa atgctacatc aaaagcatgc aaactctttc ccgcaagggt gaaaagatct
    24721 ttacggacta taaaattaac aggacgcaac ctatcagcat aaaatactcc aacaactcca
    24781 ttgggcttac gtttacctcg cccacataca acgcaggtga aattgcatat agctacaagc
    24841 tcgaaggtta cgacaccgga tggtccgagt ggagcagcaa tcccataaaa gaatttacca
    24901 acctacctta cggaaactac acctttaggc taaaagcatt ggatgccaag ggtagagaga
    24961 gcgatgaagt tgagcttaag tttaccatca gcaacccatg gtacctttcg ctttgggcat
    25021 ttatagcata cggaataacc tgcgttctga taaccctcat acttagagca gcagttaagg
    25081 ctaaaattag aaatgctacc actaaggtta aggaacaaaa aaaccttgaa atgaaagtta
    25141 aggaggagga gcataaaagg gaggttctgg aagcagagca ggaaataata aggctacaaa
    25201 atgaaaattt aaaggttcag gttgaacata aaaacagcga gttggctagc attgcgctcc
    25261 aaattgcaca caaaaacgaa tttttaaatc agctaaaatg caagctggag gctcttacaa
    25321 aaaatacaaa cccggcctta caaaaggaag tgctggagct gataaataag attgattttg
    25381 acttaaggat ggatgacgag tggaagcgct ttgaatttca cttcgatgaa gtccatgttg
    25441 gatttttgaa acgcctgaaa gacctatacc ctaccctttc acccaacgaa cttaggcttt
    25501 gcgcctatct caggctaaac atgactacta aggacattgc ccaaatactg aacatcagca
    25561 taagaggagt ggaaattagc cgctaccggt tgcgcaaaaa gttgggcatc gaaggcgata
    25621 ctaacctgat agactttatg ataaaccttt agggaaaaga ggattcagcg agtactatag
    25681 cttcacgagt ttaactcttg ctgaaaaacc ttcactattg agagtggtaa caacataaac
    25741 ccctgaagga agttgctttg tggaaataaa gcatgggttc tgacttgatt caaaatagcc
    25801 tgccaaagat ccctcaatag tgtaaatgct gacctggtag gttgtaacta cctgactatc
    25861 aaaataaaga caaatgtaat cattgcatgg gttaggataa acgcttaaaa attgagactt
    25921 ctgctgaata tagcttgctg aaagaggatg ggtgctgata gctatatcgc ttagccatag
    25981 ggaatcagct gccaccttgc cataaatgcc atagtcaacg taaaacctca cgcctttaac
    26041 ctggtgctgg ttaactacct gaccgtattc cggtgagttg gacccccact tgtttttaaa
    26101 atcaaaggag taaacatgaa attgaccacc aggctcaacc ggagaaaact taaagacagg
    26161 cgaaacatcg gttagcctgt tatccgtatc aaataaatcg gctcttagca caaccgagtt
    26221 gctaaagttc cacgttttca tccaaatatt cagaacggtg aaggagctca tgtcaacagg
    26281 gtttggtaag gtgtaggcga aataaggaaa agccgaggga tcggtaatgt tataatctac
    26341 cctaagtagc gaatcctgaa gattcaggtt gatggtgttg gggccggtca gcattcctga
    26401 ccagccctct attccatcat gtttgaagtt aacaaggcta tacctgtcac cactaggagt
    26461 agttgaagtt ttgacgttgt aagtatatgt tcctcctgcc tgaggatggg taatttgaac
    26521 ggaaacctca cccgcttgct caccccagtt caccacaatg ctgtttgttc cctgcccgga
    26581 aacaatagta gcatcgttgg gaatcgtcca tatgtatgag gcatttacaa tatttggaat
    26641 ggtaaaggac acccctgttt ggttaggagc aacatagcca ggccccgtaa taaatatttg
    26701 ctcagccgaa aggctctggt aaaccctaac gtagtcaaca agcatctttt gaggccaaat
    26761 gtttgggtca accccttgag ctccgcccca gtttccacca acagcaatat tcaacagcaa
    26821 atgaaaagct ttatcgaatg gccatgtagt gtaatcggta tgctcattgg caaaagtaaa
    26881 gtacttggta ttgtcaacat agatatctat tttctcgggg ctccattcta cagcataaag
    26941 gtgataggcg gtttcggctg taggcacatt gatggttgag gtaacttgag ttccaatgac
    27001 atggttgtaa gcttcggtgt ggatgctacc atgaactaca ccaggatcgt acccaacata
    27061 ttccatgata tcaatttcac cgcttttggg ccatgaaccg taagcccaat tggtaggaag
    27121 catccaaatt gcaggccatg tacctctccc agaaggtagc ttggcgtagg cctccattct
    27181 accatatagc caatcgcctt tttggcgtgt aaccagacgg gcagatgtgt actccatccc
    27241 accatagttt tccttacgtg cctcaactac taaattcccg ttttcaaccc ttgcattttc
    27301 caggcgattt tcggtgtagt actgcagttc gttgtttccc caaccgcttc cgccaacatc
    27361 gtagccccac ttggttgcat ccggaaggcc agagtagctg aactcatcat tccaaaccat
    27421 aacttgaatt tgggaaaaga tagaacttgt tatggttaaa gcacatatta tcagtaatag
    27481 cttcttcatc actttttctt aataaacagt cagcttgaat ttaatatttc gtctcttaaa
    27541 atatagccat cgattgggaa agttaaagca aaaaaacctt cggatcgcaa aacaaatccg
    27601 aaggtttgat gcaaaattac tcctctaatc gaaaacctaa ccctaaagac gcatcggagt
    27661 ttccaccgat gaaaagcatg aaatcaccag gttctgcaat atattccatg ttttggttgt
    27721 agaacgccag atcgttggag gtgagactaa actccaccct ggccgactca ccggatttaa
    27781 tgaatatctt tttaaaacct ttcaactccc taatgggtcg agtaacgctg cccaccatat
    27841 cgcgaacata gagctgaacc acctcctcac catcatactt ccctgaatta cgaacgtcaa
    27901 cggaaacttt aagcgtttca tccatcttaa tagacgggga gctgatgctc aggttgctat
    27961 attcaaaggt ggtatagcta agaccatatc caaaagggta aagcggtgat acagggctgt
    28021 cgatatactt tgaagtgtat ctttcttcct gacgaggggg tctgccggtg cttttttgat
    28081 agtaaaatat ggggatttgc cctacatttc ttggaaacga catgggcaac ttgcccgaag
    28141 ggttgtaatc accaaaaagt acatcggcaa tggctaaacc tcccatagtg ccgggaagcc
    28201 aagcttcgag tattgcagga acagccttat ccatccattc tatggttagc gggcgaccat
    28261 tcattaccac aactaccaag ggtttatgga gagcattaag ctccattacc aaatccttct
    28321 gcactcctgg caaatcgaga aaagctcggc taagcgcttc accagacatc atggccgatt
    28381 cacctaccac aaccactacc gcatcagcct ttcgggcagc attaatggcc tcatcaaatc
    28441 cagccttatt ttcaccaaca atttcgcagc ccttagcgta gtaaatattt ccatttggca
    28501 gcttttcacg aattcccgat agcacagaaa caacatgttt gccctcacct ctacctgacc
    28561 atgttccaat tagctcgtcc tgattgtcgg ctagagggcc aactacggct atagagcgaa
    28621 tattcctact taacggaaga acgttctcat tcttaagcaa cacgagcgat ttacgggtta
    28681 cctcgcgagc aatctccaaa tgcctggggt ttagtaaaag ctctttttcg cgaatgctat
    28741 cgcaaccttt atagggatta tcgaacaaac ctagctcaaa tttcactcta aggattctgc
    28801 gaacagcttc gtcaagctgt tcaacggtta ttctaccatc ctcaaccaac tttttaagct
    28861 gggctaaata gatacgacct tccaaatcca tatccactcc tgagctcatg gccaggtatc
    28921 cggcttcata ctcattggca gcatacccgt ggtaaatcat ctcacgaata gagccccaat
    28981 cagaatcaac aaagccatta aagccccatt tttccttaag caaatcgttt accatggcct
    29041 tatttcctgt aacaggaatg ccattaagct cgttgaatga cgacatgaag gtgacaacac
    29101 ctgcatcaag cgctgattta aaaggaggca ggtaaacctc gtagagtgtt cgctcggaaa
    29161 tatctaccgt gttgtaatcc ctcccagcct gggcagcgcc ataggcaaca aaatgtttgg
    29221 cacaggcagc aatagagctg gtgtcggata
//
LOCUS       DAOPHX010000015        28808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_1590_length_28808_cov_5.686224, whole genome shotgun sequence.
ACCESSION   DAOPHX010000015 DAOPHX010000000
VERSION     DAOPHX010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 28808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28808
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1590_length_28808_cov_5.686224"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PK502_02195"
     CDS             <1..282
                     /locus_tag="PK502_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HPG99659.1"
                     /translation="KCPTCKGTGEVSPTVLLDEQIENQLSTLVTSHKLKSITLKLHPY
                     IAAFLNKGFVSKRMRWSFRYHCRINVTPMSSYHFLEYHFFDSKDTEIGI"
     gene            complement(399..1430)
                     /gene="recA"
                     /locus_tag="PK502_02200"
     CDS             complement(399..1430)
                     /gene="recA"
                     /locus_tag="PK502_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005636551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPG99660.1"
                     /translation="MEKVEKEKEKKERKEISQDKLKALQSTLDKIEKDFGKGSIMKMG
                     DKAVADVASISSGSIALDIALGIGGYPRGRVVEIFGPESSGKTTLAIHAIAEAQKLGG
                     IAAIIDAEHAFDRSYAEKLGVDVETLLISQPDNGEQALEIADNLIRSGAIDIIVIDSV
                     AALTPKAEIEGEMGDSKMGLQARLMSQALRKLTGTISKTNTCCIFINQLREKIGVMFG
                     NPETTTGGNALKFYASVRVDIRKLNQLKDGEESTGNRVRVKIVKNKLAPPFKKAEFDI
                     IFGEGISRTGEIVDLGVECNIIKKSGSWFSYGETKLGQGRDSVRQLLQDNPELASEIE
                     AKIRETLKG"
     gene            complement(1443..1919)
                     /gene="bcp"
                     /locus_tag="PK502_02205"
     CDS             complement(1443..1919)
                     /gene="bcp"
                     /locus_tag="PK502_02205"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HPG99661.1"
                     /translation="MKTTFLKPGDTAPDFTAVDQDGVEFSLSEYQGRAVVLYFYPKDN
                     TTGCTEEACSLRDGYTELKQLGLDVIGVSPDSTRSHRSFIQKHSLPFRLAADTDHRIA
                     ELYGVWAEKSMYGKTYMGIVRTTFIINGDRKITHVFDKVNTKAHAMQILEELKKTL"
     gene            complement(2162..3616)
                     /locus_tag="PK502_02210"
     CDS             complement(2162..3616)
                     /locus_tag="PK502_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HPG99662.1"
                     /translation="MRRFIARNKITLFTLCISGMLLCTYNSAHAQDDEEYYKNLLDTV
                     VEVINPVYKPVLSFGAGIMSFWGDVQNPVTNPVNGKYGFKFNVSTLFGKNYFKGNIFA
                     IYGQFQGQNFDITRQMQLQPLPLDDIGNPIYPNSSFQTEFWQVGITAEYGFGHLFGLV
                     KRFKPFIAVGVSTMFFTPKGNVRYGPTGDFYYAWSDGSLRDFPENGPNAYLAQIIKLD
                     NSYETDLSSADLYRIGSYSQNTFTIPLDLGFDFYLSDRVNFRVGATVNYAFSDLLDNY
                     SSKTASTMGYPNKNSYNDIFAFTYFSMNLDLFSDPKTMLVERVFAELSGTFDYGVFFA
                     DQDGDNVLDRFDACPDTPPGVAVDSLGCPPDYDNDGVFDYMDDESSTPEGAIVNERGV
                     QLSADVLSDMFSKNSMAVPRSEVKVIPLANVWTRNATFTPGAIPDKFRIVDADADGYI
                     SFQELLKAINDFFDEKNTFTPDEIYELNEYFFSQ"
     gene            complement(3635..5008)
                     /locus_tag="PK502_02215"
     CDS             complement(3635..5008)
                     /locus_tag="PK502_02215"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPG99663.1"
                     /translation="MARLFIVIINIIGITLAGILGGGNVLVTMKAPSRVDAGSEFDVE
                     VLLKKAGVDGFARFQQELPLGLTAELIESSLGDFTFEDQKVKVVWLKLPAAKELRFVY
                     RIRVDERLKGSFNLGGVFSYIENNERKTSSVQAAQISITPSTRINPSLIVDINEFQNV
                     IPVQAPVSMLASNVKCIRQTPIPTGEANDLLVKLLVNKGNAQKFAKIEEDIPQGFQAE
                     VVDAKDAIFTFKDQKVKFLWMSMPPDPRFIVSYRLIPINGNGEAVVSLSGKFSYLVGE
                     ATRVVDIIQKDVDLGNLDPKSIEGLLASIPGAEIAAPSSSSFSSSFTSAGSDGGIDIP
                     VQYQVIPEKSKKIAQPEQNMMLYMLEPEKGVYYRVQVAAGHKPINIKRYFLRHNVTYE
                     VRTEKHEGWYKYSIGSFKEYKEARDFRVQIWNTTKINDAFVAAYNNGVRITVQEALMI
                     TNQTWFR"
     gene            complement(5192..6862)
                     /locus_tag="PK502_02220"
     CDS             complement(5192..6862)
                     /locus_tag="PK502_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HPG99664.1"
                     /translation="MRREVEIVLENTNQKVRITPGLSLMELTGIFNIKLEHQVLGAMV
                     NNQLKELDYEIFTPKTIRFIDITHPAGMRMYQRSLFFLLHKAVGDILPGCKVRIEHSV
                     SKGFYCEIDGFEQPLDLPLVFSICDRMREVVAADIPFRRDKILTTDAVKLFEDLGYTE
                     QAKLFDTRPKLYTSIYYLGDLPGYFFGNLVPSTGYLKVFDLVKYYQGMLLRIPKRSKP
                     DEVEDVVIQNKMFDIFQEYKDWTHILGVGNVVAINEQVEQGNASELIKVSEALHEKKV
                     AYIADKIHSLGDKVKVILISGPSSSGKTTFAKRLAVQLRVNGIRPYTISLDNYFVDRD
                     RTPRDENGDFDFEALEALDIEYFNSDLLRLLNGETVELPKFSFEKGARFFDNTLMRID
                     PNGMIIIEGIHALNPKLTPLIPSDKKFKIYVSALTSMSYDGINRIPTTDNRLIRRIVR
                     DYRYRGYNALQTIARWESVRRGEDRNIFPYQEEADEMFNSALLFEFGLLKSYVEPLLQ
                     EVPPNRPEFDEADRLLKFLYYFNTISDSEVPPTSIIREFLGGSAFSYS"
     gene            complement(6903..7724)
                     /gene="murI"
                     /locus_tag="PK502_02225"
     CDS             complement(6903..7724)
                     /gene="murI"
                     /locus_tag="PK502_02225"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPG99665.1"
                     /translation="MLGSNPIGIFDSGSGGLTVLYELLKVLPRESFVYFADSANCPYG
                     PKPQSEVIELSDCITRFLLEQGCKLVVVACNTATAAAIEHLRSNFKIPFIGMEPAIKP
                     ASLNSKTKSIGVLATAGTFKGKLYIETSKKYASDVNVCYQVGEGLVELVEAGKADSPE
                     AAELLHRYVDPMVDCNIDHLVLGCTHYPFLMNSLSRILPRGVEIVNPAPAVARQTLRV
                     LSENNLLNDGSSLSKVSFYSSASVEILKYLAEKLGVISNHGPAVNFTGGVKLCCR"
     gene            complement(7901..8419)
                     /locus_tag="PK502_02230"
     CDS             complement(7901..8419)
                     /locus_tag="PK502_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPG99666.1"
                     /translation="MRKISTIAFILFLSATGVKTYAQSYKFGHINSQEILSVMPERDS
                     AVAKLDKFTKTLQDQVEELQVEFNKKYQDYLQKRSTFTDAIREMKEKELTEMQQRAQE
                     FQQVAEQDYQRYQGEVMKPVIEKADSAIKKVAKANGFTYIFDTSTGVILYFSEQSVDI
                     GPLVKKELGITK"
     gene            complement(8458..8967)
                     /locus_tag="PK502_02235"
     CDS             complement(8458..8967)
                     /locus_tag="PK502_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPG99667.1"
                     /translation="MKKILLLPIVLLISTSLWAQKFAFVDSEYILNKIPSYKAAQEQL
                     DKLSEQYQKEIEGKYAEIDKMYKDYQTEKVLLTQEMQKKREDEIISRERQTKELQMKY
                     FGRDGLLFKKREELVKPIQDQVFNAVKEIAIEGGYAIIFDAAASPNMLYTNPRYDKSE
                     DVLLKLGYK"
     gene            complement(9154..11631)
                     /gene="bamA"
                     /locus_tag="PK502_02240"
     CDS             complement(9154..11631)
                     /gene="bamA"
                     /locus_tag="PK502_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPG99668.1"
                     /translation="MLKSKIFLLLLLLSSVSALAQEPSFSYEVPKKYCVKEVRVSGIK
                     FLNPDVLVSVSGISVGDSILVPGDAVTKAIKKLWSQGLFSDIKISATKIENSDIYLDI
                     FLQEQPRISSINFVGVRKGEVSDLKEKLKLRNGSQLTESILENSVEIIRKHYRTKGFL
                     NVDVNPIQENDTALANMVKLTFDIRKNERVKIDQITFDGNTAFTDARLRKSLKKIHRR
                     DINIFKSAKFIQSDYDESKDNLIAFYNENGYRDSKILKDSVFTLNNKRIGINFLVHEG
                     PQYHIRSISWIGNTKLPSEALTAILGMKKGDVYDKTLLEKRLFTDDNSISTVYMDDGY
                     LFFNLDPVESQIQNDSVDLEMRIYEGDQATINKVLIVGNTKTNEHVIRRELWTKPGYL
                     FSKSEITRTIRELGQMGHFDPEKLDVQPMPNPAEGTVDLKYVVEEKANDQLEISGGWG
                     NKMFVGTVGIRFTNFSVRRLFDKEAWRPVPSGDSQSLSLRASTNGTYYKAFSFSFTEP
                     WLGGRKPTNFSFSVYHTIQNGGVDYFYQTSDKYFKVTGASVGIGTRLKWPDDYFSIYN
                     EISYQNYNLKDWTGYFLFSNGQSNNLSYKISLTRNSTDQVIYPRTGSNLSLSLQITPP
                     YSLFNGKNYSALSGSEKYKWIEYHKWTGRIQWYVPLVEKLVLYSNFQFGIMGYFNSDI
                     GHSPFEGFDVGGDGMSGYNLYGKETIGLRGYKNGSLTPYTLIDGSYVGDGHVYDKYTV
                     EIRYPLTLNPQAAIYLLTFFEAGNAWRDASEFNPFNVHRSAGIGARMFLPMLGMLGID
                     WGYGFDKIPDNPDAHKGQFHFIIGMPF"
     gene            complement(11769..12506)
                     /locus_tag="PK502_02245"
     CDS             complement(11769..12506)
                     /locus_tag="PK502_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPG99669.1"
                     /translation="MDIKNQIDKNNVPQHVAIIMDGNGRWAEKRGLPRIFGHKNGVGA
                     VRAATEAAGEIGVKFLTLYAFSTENWKRPKIEIDTLMNLLIETIDNEIGNLNKNDVRL
                     ITIGNTSALPKNVQTKLKYAIDKTSQGKTLTTILALNYGARAEITEATRKILTEYRNG
                     KLDEMSLSPEVFGGYLQTAGIPDPELLIRTSGEIRLSNFLLWQLAYAELYFTPVLWPD
                     FSKDDFFNAIVEYQHRERRFGMTSEQL"
     gene            complement(12519..13196)
                     /locus_tag="PK502_02250"
     CDS             complement(12519..13196)
                     /locus_tag="PK502_02250"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HPG99670.1"
                     /translation="MDILANRIFFSFLSSLLVLSFFLLLPFKSHGQDKRDFGVQAGST
                     YYYGDFNEAKPLYQPSFAMGVIFRYNFNTQYSIRASALYGAIKGESPSGFFLPLSAST
                     TFSNTLLEAEVMGEFNFVSFSPLSTRKKKLSPYVNIGLGVGQLSGSVLFHIPFGVGLK
                     FSPGKRHTLAMEWRFHKTFNDELDSYAAPDDGKTPFLHNNDWFSYIGLIYTYRLYNSG
                     NVCPVYK"
     gene            complement(13184..13942)
                     /locus_tag="PK502_02255"
     CDS             complement(13184..13942)
                     /locus_tag="PK502_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HPG99671.1"
                     /translation="MKKSFIVVGIILFLCLPGHSQVWKRNRIEIFLGVPITHYFGDIG
                     GTSDPSSMMGIKDISIRAIRPGFSGGLGYRLTERFYAQGTLTLGLFGNSDKGSRNEAR
                     NYAFSTFGTELAAVAQFYIIPESDQNYFYSIMQVRGGLRKINKPFSLYLFAGGGGCFY
                     RVKPLNDLVDSDRFDDSQHTAVVVPFGLGFKYQIHSRMLLGVELGGRYVLSDYLDGFS
                     PEPSKYNDFYYVFNFKVHYRLPSNKILRKPQWTF"
     gene            complement(14026..14904)
                     /locus_tag="PK502_02260"
     CDS             complement(14026..14904)
                     /locus_tag="PK502_02260"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HPG99672.1"
                     /translation="MVVAIYGKAVETEYLPSLGELFNQLVEHNVEVWVYRPFMEYLLE
                     TFSAKLPDFEVFNTGSDINGKANILISIGGDGTFLDSVSFVRSSGLPIMGINFGRLGF
                     LANIAIPDMHNAIDLLIAGRFKVEHRSMLEVVSTLEQFNTFPFGLNDFTMQKAGSGFL
                     KVNAYVNGEYLCAYWADGLIVSTPTGSTAYSLSVGGPIVSPNLSALILSAIAPHNLTV
                     RPLVISDDNILLLEAVGRDGSVMLSLDSRSITCKSPVTVTVKKAPFFANVVSMEGSNF
                     YHTLRGKLLWGMDKRN"
     gene            complement(14923..15588)
                     /locus_tag="PK502_02265"
     CDS             complement(14923..15588)
                     /locus_tag="PK502_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPG99673.1"
                     /translation="MIALEMISDVIPALKTSDTGQTALNWMDIFKVSHLPIVNNKEFL
                     GLVSESDIYDLNMPDEPLGNHYLSLNSPYVIEDQHIFEVMEVLSRLKLSLVPVLDSGK
                     NYLGVITLMELLHYFAVESGISLQGGIVVLEMNANDFSMSQVCQIVEGNDAKILAAYV
                     TPHPNSTRMELTLKLNVTDLTSIRQTFNRYNYTVKASFLKQDDEADMLNDRINFLFRY
                     LDI"
     gene            15650..16366
                     /locus_tag="PK502_02270"
     CDS             15650..16366
                     /locus_tag="PK502_02270"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HPG99674.1"
                     /translation="MTRLSVNINKIATLRNARGGNIPDVLKAAIDCQRFGAEGITVHP
                     RPDERHIRYQDVRDIKPIITTEFNIEGYPVESFIELVLSVKPQQVTLVPDPPDAITSS
                     SGWDTINNKDLLKRMVATFKREGIRTSIFVNADPEMVKHAADTGTDRVELYTEPYASM
                     FKVNPEKAIEPFITAADAAKQVGLGLNAGHDLNLENLSYFNRHIPNLLEVSIGHALIS
                     DALYYGLENTIQLYLQQLRS"
     gene            16366..17169
                     /locus_tag="PK502_02275"
     CDS             16366..17169
                     /locus_tag="PK502_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPG99675.1"
                     /translation="MKLFYRELGKGFPLVILHGLYGSSDNWMGIAKRLSTSHRIILPD
                     QRNHGQSPHSSSHTYPDMANDLAELVDNLGIDNFHLLGHSMGGRTAMLYQSLFPYMVK
                     SLVVADIAPWPYQPNDEVFRLSLAEHSSIIKGMLSLPLTSIKSRYEAEKMLTGSIPSQ
                     KLRQFLLKNLKRDTSGSYSWCLNLPVLESSLRELITGVNLECGKKSPTRVLFVKGENS
                     NYLPENSEVRLTDYYPDCRLVTIRNATHWLHAEQPEAFTKSILEFLQKG"
     gene            complement(17166..17888)
                     /locus_tag="PK502_02280"
     CDS             complement(17166..17888)
                     /locus_tag="PK502_02280"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPG99676.1"
                     /translation="MLKDVSPENILFLDIETVPAYPAYTDLPEADRKLWDKKAAQLVK
                     DHETAADIYGRAGIYAEFGKIICISTGLVVSKVEERFFRIKSFFGIDEKILLADFAEM
                     LSRFEKRKDVNLCAHNGKEFDFPYIARRMLVNDIRIPDILDVSGKKPWEVRFLDTMEL
                     WKFGDNKHYTSLNLLTHIFGILSPKDDIDGSQVAECYYLQNDLNRIATYCEKDTLAVA
                     QLFMRYKGLPQIAPENVERVNG"
     gene            complement(17891..18514)
                     /gene="udk"
                     /locus_tag="PK502_02285"
     CDS             complement(17891..18514)
                     /gene="udk"
                     /locus_tag="PK502_02285"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HPG99677.1"
                     /translation="MLIVGIAGGTGSGKTTVVNRIIDLLPKGEVVVLPQDAYYRDSSH
                     LPLEQRQEINFDHPDSIEFELLVEHVKLLKEGKAVEQPIYSYLTCTRARETITIKPRH
                     VVIIEGILIFTCPELRNLLDIKVFVDADADDRLSRVIARDIVERGRSVNKVLDRYEKT
                     VKPMHLQFIEPTKRYADIIVPQGGNNAVAISILASIIEKTLRNHNLV"
     gene            18605..19594
                     /locus_tag="PK502_02290"
     CDS             18605..19594
                     /locus_tag="PK502_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HPG99678.1"
                     /translation="MENTGLYPLRFKPIFKPRIWGGNKLRQRLNKNIPKNISNCGESW
                     EVSAVGDNISVIANGFLEGNNLEELVEIYMGDLVGNSVYEKFGIELPLLVKFIDASDN
                     LSIQVHPNDELARERHHAYGKTEMWYVIDADPGAKIITGFNRDVTREEYLESLSTGKL
                     TDLLNVETALAGDVYYIPAGRVHAIGRGVLLLEIQQTSDITYRIYDWDRLDETGKPRE
                     LHTSLALDAIDFSKVSNPKRNITESLNTPIELASCPYFTVNKLTLTQPLKRDYFMIDS
                     FVLYICLAGDVLVNYPNGKEKLATGETMLIPAEIKNISLQPVSTCTIIEVFVG"
     gene            19725..20312
                     /locus_tag="PK502_02295"
     CDS             19725..20312
                     /locus_tag="PK502_02295"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HPG99679.1"
                     /translation="MDNIIYFIKSTLASEIASSGFPLIYKGEMNHQIMRSFAFMANRK
                     ISDMDVPTSIRKRVFHIMIECLQNITKHSDDYDEKERQIGNGLFVVGREKDSFFIVTG
                     NLIRNDKMAPLEERLISLNNASPTELKSLFLQQMIGGELTEKGGAGLGLIDIARKSKR
                     KLYYHFVPYENNRHFFILAVTIPIEIDEDDNDENL"
     gene            20541..21518
                     /locus_tag="PK502_02300"
     CDS             20541..21518
                     /locus_tag="PK502_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99680.1"
                     /translation="MKRYLATLCLATTCTIVLAQSRSELMSREKIQKNKIASVSQWTH
                     KFNKDKVDPKGYVTVVTNYDKNGNEVEIINYKQNGSVSSRHTYKYDNQNRKIEYLQYQ
                     DYRQKGIELAFRQVFTYDKNGNKASELGFDGRTTYRIAYSYFPNGKQKEIIRYNAANQ
                     VEEKWIYEHNGNNTKISIFKPVGTLSKITERKYDSAGNLLDEKNTSGDGKELARTVFT
                     YNANNLPISKSEYYAGELRASYSYVYDGQNQLVETYLIKPNNTRLLYSSYKYDGQGNI
                     VEEKWYEDNATDYSHRNYKLDSKGIVDEVDTYYSDYNFRVVYRYEYKFY"
     gene            21896..25246
                     /locus_tag="PK502_02305"
     CDS             21896..25246
                     /locus_tag="PK502_02305"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPG99681.1"
                     /translation="MANKSGLLHTSYTLLLVLIFQVNAIWAQTTGRFPVMNFHHREYN
                     GHAQSWAITQDNRGLIYIANNVGVIEYDGSEWRHISINGALARCLDTDKNGRIWVGGQ
                     DEFGYLAADSTNTLSFHSLSQHIPETAKPLGMIRQLYASDSSIYFSSFTTLFRLNKDL
                     TVSEWSPKTQFHRSYLTDGVLFIHQRDYGLCYLSNDSLRLVPGGDYFAPQLIYAILPF
                     NQKYFLICTQNDGFYLLSRQALDPNFTGYTKPAIVKFSTSDDNFFASSKVYAGLKLDD
                     NTFAISTYSGGVVFIDTTGNITRRIDTSVGLQDNTAWYLYMDNQKNLWIALNNGISYT
                     PIFSQLTSWTENDGIKGVPQSVVRFKNHIYFTTNVGIFWFSNGRIEQVTGLSDLSWKL
                     RTIRTMNGDEALLATTSSGIFQVEGGKATLIENGKYHSYCLLQSKLYPKITYVGLREG
                     VGVIAENNGRFKFLGELTGTKGEVYSLAEDLNGDLWYSERYKGINYVDMVNPYQLVSE
                     KPILYTLPFNPRYDDIAVVMIDGEVKASAECGLSKFSHQSNMFEPDSSLGLEFANGTT
                     GLRILNQDQNGNIWFEAYNFSANRWLERAVKYPKNIYRREPAQFRTIPETIFYDIFPE
                     DNNKVVWIACTDGLFRFDENAGNLKNPIFRVLIRQVSAGPDHILFNGVYYDGNEGITF
                     PTSLPYNEVKSTTLPYKNNSLAFTYSAPFFGQQQKLLYSYMLVGFDKTWSDWQPNQRK
                     EYTNLPFGTYQFNVKAKNLFNIESPAETFQFTIQKPLYLQWWAITGYVVALLLIIWLS
                     IRINTQLLRNSNIKLQQLVDERTLELSKSEQALIEKNVELQRQKEEILTQRDELEEHN
                     RHINDSIIYAQTIQQAILPDLSQSLGNIFEHFLIYKPKELVSGDFYWISHAGPKNNPN
                     EKIFIAVVDCTGHGVPGAFMSLIGSRLLSEIVNEKKIYNPADILTELSASIKVALHQN
                     VSESFDGMDIALCLIESKPNDMFSVTFAGANRPIFCYSKAENRIQTLKGNRKSIGGLL
                     PDVDLEFTNWRVPMHKGDMIIMYTDGLTDQNNSSKRKFTTARFQTYLLANIEKPMEEI
                     SSILLQEFNEFTGSEPQRDDITILGIRL"
     gene            25311..27398
                     /locus_tag="PK502_02310"
     CDS             25311..27398
                     /locus_tag="PK502_02310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPG99682.1"
                     /translation="MQTKKILMALALPALVLASCKTQQPNNEAIVGPVKPVVTDSTLT
                     PEILWSFGRIGEFAVSPDGQKVAYTTAWYSIDQNRGNSEIYIMNTDGTGKRQLTHTNG
                     SEFNLQWVDSLNLAYLATESGKSQIWQMNVENQKTSMVEGFENDISGFSISPTGSKIL
                     LTSDVKVGASIADIYPDLPKSSGQIYTTLMYRHWDTWEDSLFNHVMVANFDGKSLSKI
                     IDIMANEPYDTPLKPFDGMEDIAWSPDGNQIAYTCKKLTGKEAAFSTNSDIYLYDLRN
                     GKTQNLTEGLPGYDKQPVFSPDGEKIAWLSMERAGFEADKDRLLVMDLQTKKTSYLTR
                     DFDYSVSNVIWNASSDMVYFIAGVEATYQIYRLALQDAKPVAITHGWHDYHAFALAGD
                     RLIGSKASIVKPVEIYSINPQNGEEVELSFTNQELLSQLKMPTVRQRWIKSTDGKLVH
                     TWIILPPDFDSTKSYPALLYCEGGPQSAVSQFWSYRWNFSIMASGGYVVVAPSRRGTL
                     TFGQKWTDEISKNHGGQDMKDLLSAIDEVKKEPWVDENRLGAVGASYGGYSVFWLAGN
                     HNKRFKAFIAHCGVFHSEMEYLTTEEMFFDTWEMGGAPWEKNNPVAMKSYAQSPHNFI
                     TSWDTPILIISGGNDFRIPYTQGMAAFNAAQILGVPSKFIFFPDENHWVLKPQNGILW
                     QREFRSWLDRWLK"
     gene            complement(27535..28266)
                     /locus_tag="PK502_02315"
     CDS             complement(27535..28266)
                     /locus_tag="PK502_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG99683.1"
                     /translation="MSSTNLITLNEIKKFYQVGSVTVKALKGINLTIDQNEYVAIMGP
                     SGSGKSTLMNILGCLDTPTDGTYILNGTDVSRLSDDELADIRNKEIGFVFQTFNLLPR
                     YTSLDNVILPLIYAGVPKHERVEKAKQVLYNVGLGDRMEHKPNELSGGQRQRVAVARA
                     LVNNPSIILADEPTGNLDSKTSIDIMNLFEDIYSQGNTIIIVTHEEDIARHARRIVRI
                     LDGMIESDEVNPHPTLRQKVVLTEQ"
     gene            complement(28333..>28808)
                     /locus_tag="PK502_02320"
     CDS             complement(28333..>28808)
                     /locus_tag="PK502_02320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPG99684.1"
                     /translation="EIIVELGTSVGISTMFMAAANPNSKVYTIEGEESILAIARNRFK
                     QMGFTNIEPIAGNFDVQLPELIKRISTIDFAFIDGDHNGRALLQNFNRMLPRVADTSV
                     FVIDDIRWSPGMEAAWKQICSNPKVTISIDLFRCGILFFRTGIAKQHFLLRYGPY"
BASE COUNT         8265 a         6573 c         6248 g         7722 t
ORIGIN      
        1 aaatgcccca cgtgcaaggg gacaggagag gttagcccca ctgttctgct cgacgagcag
       61 atcgaaaatc aactttccac gctggttacc agccacaagt taaaaagtat tacccttaag
      121 ctgcacccct acattgcagc tttcctaaac aagggttttg tttcaaagcg tatgcggtgg
      181 tcgtttaggt accactgcag gataaatgta actcccatgt cgtcctacca ctttctggag
      241 taccattttt ttgactccaa ggataccgag ataggtatat agccaacaaa aaaaccccgc
      301 tgccaggcag cggggttaac tttatgcagt aacaaagtct gcatcaatag gagtggtaaa
      361 aatatcagat ttttgttgcc aaacgctcta cctgaccgct aaccctttaa cgtttcgcga
      421 attttggcct caatttcgct ggccaactcg ggattgtcct gtaggagctg gcgaacgcta
      481 tcgcgacctt gtcccagctt tgtttcaccg tagctgaacc acgatccact ttttttaata
      541 atgttacact ccacaccaag atcaacaatt tcaccggttc gtgagattcc ttctccaaaa
      601 atgatgtcga actcggcttt cttgaacgga ggagccagct tattctttac aattttcacc
      661 ctcacccggt taccggttga ttcctcccca tccttaagct ggttgagctt acgaatatca
      721 accctaaccg aagcgtaaaa cttaagggca ttccccccgg tagtggtttc gggatttccg
      781 aacataacgc cgattttttc acgcagctgg ttgataaaaa tgcagcaggt gttagttttg
      841 ctaattgttc cggttagctt tcgaagggcc tgtgacataa gccttgcctg aaggcccatt
      901 ttgctatcac ccatttcccc ctcaatttct gccttagggg taagggctgc caccgagtcg
      961 ataacaatta tatcaattgc ccctgagcgg ataagattat cggctatttc aagcgcctgt
     1021 tctccgttgt cgggttgcga aatcaacagt gtttccacat caactcccag tttctcggcg
     1081 tatgagcggt caaaagcatg ctcagcatca attatggcgg caatgccacc aagtttttga
     1141 gcttcggcaa tggcgtgaat ggccagggtt gtttttcccg acgattcggg tccgaatatt
     1201 tcaaccaccc tgcctctagg gtatcctcca atgccaagcg ctatatccag agcaattgac
     1261 cccgaggata ttgaggcaac atcagcaact gccttatcac ccattttcat gatggacccc
     1321 ttcccaaaat ctttttcaat tttgtcgagg gttgactgaa gagccttcag cttatcctga
     1381 cttatctcct tgcgctcttt cttttctttt tctttctcta ccttttccat gtggttggta
     1441 tgttaaagcg tttttttcaa ctcctctaaa atttgcatag catgggcttt agtattgacc
     1501 ttgtcaaaaa catgggtgat cttccggtcg ccattaatta tgaaggtggt gcgaacaatt
     1561 cccatgtaag ttttgccata cattgatttc tcagcccaaa ccccatacaa ctcagcaatc
     1621 cgatggtcgg tgtcagcagc caacctgaag ggaaggctgt gcttctggat aaatgaccta
     1681 tgcgacctgg tgctgtcggg gcttaccccg ataacgtcaa gccccagctg tttaagttcc
     1741 gtgtacccat cccttaggct gcaagcctct tcggtgcagc cagttgtgtt atctttggga
     1801 taaaagtaaa gcactactgc tctgccctgg tactcggaaa ggctgaattc aacgccatcc
     1861 tgatccactg ctgtaaagtc aggagctgta tcgccaggtt ttagaaaggt cgttttcatc
     1921 actttgattt tgtaaagtta tgaagaatat acaaaatagc tctcgggttg ttcaaaaaat
     1981 ctaccaaaga cttatcaaca gaaaataccg taaccttcat ttgcttgttc ctgacaaaat
     2041 ttatcaccct gaagttaaaa aaaaccctac aggaattctg tagggcaggt agtaaaaggg
     2101 ttttaggtta ggttatatag ggttgtttgg gtttataggt taattggttt taggtttttg
     2161 cttactgtga gaaaaagtat tcgttgagct cataaatttc atcgggggta aaggtgtttt
     2221 tctcatcaaa gaagtcgtta attgccttga gcagctcctg gaaagagata tatccatcag
     2281 catctgcatc aacgatgcgg aatttatcgg gaattgcacc aggagtaaag gttgcgtttc
     2341 gtgtccagac atttgcgagg ggaataacct taacctcact tctgggcact gccatggagt
     2401 ttttgctaaa catatcggaa agcacatcag ccgagagctg cactcccctc tcgttgacaa
     2461 ttgctccctc aggggtactg gactcatcgt ccatatagtc gaatactccg tcgttgtcat
     2521 aatcgggtgg gcaaccaagc gaatccacgg ccactcctgg aggtgtatcg ggacatgcat
     2581 caaacctatc caacacatta tcgccatcct gatcggcaaa gaaaaccccg tagtcaaatg
     2641 taccactaag ttcagcaaac accctctcga ccagcatggt ctttgggtcg gagaaaaggt
     2701 cgaggttcat gctgaaatag gtgaatgcga atatatcgtt gtagctgttt ttattggggt
     2761 agcccatggt gctggcagtt ttgctgctat agttgtccag caaatctgaa aaggcatagt
     2821 taacggtagc acctacccta aagttaaccc tgtcggacag gtaaaaatcg aaacccaggt
     2881 caagtggaat ggtaaaggta ttctggctgt aggaaccaat tctgtacaaa tcggcgctgg
     2941 aaaggtctgt ttcgtagcta ttatcgagtt tgattatttg agctaagtag gcattaggac
     3001 cattttcggg gaaatcacga agcgaaccgt cggaccaagc atagtagaag tcaccggtag
     3061 gcccatacct gacatttccc ttaggcgtaa aaaacatagt tgaaacgcca actgcgataa
     3121 atggtttgaa gcgcttaacc agcccaaata gatgaccgaa accatactca gcagttatgc
     3181 caacctgcca gaactcggtt tggaatgagg agtttggata aatgggattt ccaatatcgt
     3241 ctaggggtaa tggttgtagc tgcatctgac gggtaatatc aaagttctga ccctgaaact
     3301 ggccgtaaat cgcaaaaata tttcccttaa agtagttctt cccaaaaagc gttgaaacgt
     3361 taaatttgaa accatacttt ccgttaacag gattggttac agggttttga acgtcccccc
     3421 aaaaactcat gattcctgca ccaaatgata gaaccggttt gtaaacgggg ttaataacct
     3481 caacaaccgt atcgagcaag tttttgtagt attcctcatc atcctgtgca tgggctgaat
     3541 tataggtgca aaggagcata cccgaaatgc agagggtaaa gagtgtaatt ttatttctgg
     3601 caataaacct tctcatcaga actttaacag ctagctatct gaaccaagtt tggttggtta
     3661 tcattagtgc ttcctgaacg gtaattctaa caccgttgtt gtaggcggcc acaaacgcat
     3721 cgttaatttt agtggtattc catatttgca ccctaaagtc gcgcgcttcc ttgtactcct
     3781 taaacgatcc tatggagtat ttataccaac cctcatgctt ctcggttcgc acctcgtagg
     3841 taacattatg tcgtaaaaaa taccttttga tgttgatggg cttatgcccg gcagccacct
     3901 gaaccctgta gtaaacccct ttttcaggct ccagcatgta gagcatcatg ttttgttcgg
     3961 gctgggctat tttttttgat ttctctggaa ttacctggta ctgaacagga atatctattc
     4021 caccatcgga gccagcagat gtaaagctgc tgctgaagct tgaagaagag ggagctgcaa
     4081 tttcagcgcc cgggatggag gccagcaatc cctcgatgga tttcgggtcg agattgccca
     4141 agtcaacatc cttttggata atgtcaacta cccgtgtagc ctcaccaaca aggtaggaaa
     4201 attttcccga aaggctgaca acggcttctc cgtttccgtt aataggaatt agccggtacg
     4261 acacaatgaa gcgagggtcg ggcggcatac tcatccatag aaatttaacc ttttgatctt
     4321 taaaagtaaa aatggcatcc ttggcatcta ccacctctgc ttgaaagccc tggggtatat
     4381 cctcctcaat ctttgcaaat ttctgggcgt tacccttatt aaccaatagt tttaccagca
     4441 aatcgttagc ctcgccggtg ggtatagggg tttgcctaat gcacttaacg ttcgaagcca
     4501 gcatagaaac aggggcttga acgggaatga cattctgaaa ctcgttgata tcaactatca
     4561 gcgaagggtt gatacgagtt gaaggggtga tagaaatctg tgctgcctgt acggaagaag
     4621 ttttacgctc gttattttca atatatgaga aaacgccacc aaggttgaaa ctccccttta
     4681 aacgctcgtc aacccgaatt cggtaaacaa atcgtaactc cttggctgca ggaagcttaa
     4741 gccatactac ctttaccttt tgatcctcaa aagtaaaatc gccaagggat gattctatta
     4801 gttcagcagt gaggcctagg ggtaattcct gctgaaatcg ggcaaaacca tcaacaccag
     4861 ccttttttag gagcacttcc acatcaaatt ccgaacccgc atctacccga cttggagctt
     4921 tcatggtaac cagcacatta ccacctccca aaatgccagc aagcgtaata cctattatgt
     4981 tgataataac tataaatagc cttgccatta tagcttggat tcaggtttag ggttaatttg
     5041 ttacaattat agtgaataat tataattaaa agcaagagaa caaggtaaaa atcatgggaa
     5101 gcagcaggtt aaggcaaaaa atatataaac aaaataattt cattgaattg attttcaact
     5161 atatagcatg ttaataactt aacatggcat tttaagagta ggagaaagcg ctacccccta
     5221 aaaattctct tataatcgag gttggaggaa cctcgctgtc tgaaatggta ttgaaatagt
     5281 aaaggaattt aagcaggcgg tcggcttcat caaattcagg tctattgggc ggcacttcct
     5341 gaagcagggg ctcaacatag ctcttaagaa ggccaaactc gaaaagcagg gcagagttaa
     5401 acatttcgtc agcctcctcc tggtagggaa atatgttcct atcctctccc ctacggacac
     5461 tctcccagcg ggcgattgtt tgaagggcgt tgtaacccct gtaccggtag tcgcggacaa
     5521 tccttctgat aagcctgtta tcggtggttg gaatacggtt aattccgtcg tacgacatgg
     5581 aggtcaacgc tgaaacgtat attttaaact ttttgtcgga tgggattaac ggggtgagct
     5641 ttgggttaag ggcatgaata ccctcaatga taatcatgcc gttaggatcg attctcatta
     5701 gggtgttatc aaagaatcgg gcgcccttct cgaacgagaa tttaggaagc tcaaccgttt
     5761 caccgttaag caacctgagc aagtcgctgt taaagtattc tatatcaagt gcttctaatg
     5821 cttcgaagtc gaaatcccca ttctcgtccc ggggggttct atcccgatcg acaaaatagt
     5881 tgtcaagcga gatggtgtag ggtctgatgc cgtttaccct taactgcaca gcaagccgct
     5941 tggcaaatgt tgttttgccc gacgaggatg ggccagaaat taggataact ttaactttgt
     6001 cgcccagcga gtggatttta tcggcgatgt aggcaacctt tttttcatgt agggcctcgg
     6061 aaacctttat cagctcgctg gcatttccct gttcaacctg ttcgttaata gcaacaacgt
     6121 tacctacccc cagaatatga gtccagtcct tatactcctg aaaaatatcg aacattttat
     6181 tttgaataac tacatcctcc acctcgtcgg gctttgaacg ctttggaatg cggaggagca
     6241 taccctggta gtacttaacc aggtcgaata cttttaggta gccggttgaa ggaaccaaat
     6301 tcccaaaaaa gtaaccgggt aaatcaccca ggtagtaaat tgaggtgtaa agttttggcc
     6361 gggtgtcaaa gagcttggcc tgctcggtgt agcccagatc ttcaaagagt tttactgcat
     6421 cggtggtaag gattttatct cttcgaaatg ggatatctgc agcaacaacc tcgcgcatcc
     6481 tgtcgcaaat tgaaaacacc aggggcaagt caagcggctg ctcgaagcca tcgatctcgc
     6541 aataaaatcc cttagaaaca gaatgttcaa tccttacctt gcagccgggc agtatgtccc
     6601 cgacagcttt atgcagcagg aaaaaaaggg agcgttggta catacgcatt cctgcaggat
     6661 gggtaatatc aataaatcta attgttttgg gagtaaaaat ttcgtagtcc agctccttca
     6721 gctggttgtt taccatagcg cccagcacct ggtgctccag cttgatattg aatatgccgg
     6781 ttagctccat aagcgacaaa cctggggtta tccgaacctt ttggttagtg ttctccagca
     6841 caatttcaac ttctcttctc atatgtagta gggtaagttt aagaatcaat aaaataggtg
     6901 cattaacgac agcaaagttt tacacctccg gtaaagttga ctgccggtcc atggttagaa
     6961 attacaccga gtttttcggc gaggtatttc aaaatttcca cggaggcgct tgagtagaag
     7021 cttaccttgc tcaaggagct accatcgttt aaaaggttgt tttcggaaag cacgcgaagt
     7081 gtctgccggg caacagcggg tgcaggatta acaatttcaa cccctctagg cagtatccgg
     7141 cttagggagt tcatcaaaaa agggtaatgg gtgcacccta aaaccagatg gtcgatgttg
     7201 caatctacca ttgggtcaac atacctatga aggagctcgg cagcttcggg cgaatcggcc
     7261 ttgccggcct caaccagctc aaccaaaccc tctcccacct ggtagcagac attcacatca
     7321 gaggcatatt ttttactggt ttcgatgtaa agtttcccct taaaagttcc ggcagttgcc
     7381 aacacgccaa tactcttggt tttggaatta agtgaggcgg gttttatagc cggctccatg
     7441 ccaataaagg gaattttaaa gttacttcga aggtgctcaa tagctgcagc ggttgcggtg
     7501 ttgcatgcaa caaccaccag cttgcaaccc tgttccagca aaaaacgggt aatgcagtca
     7561 gaaagttcaa tcacctccga ctggggtttg ggcccgtatg ggcaatttgc cgaatcggca
     7621 aagtaaacaa aggattcccg gggcagtact tttaaaagtt catacagcac cgttaatccg
     7681 cctgacccgg agtcaaaaat tcctatagga ttactaccaa gcaattaacg ataaccttaa
     7741 tttgggaaaa atgaaatatt catgacaata gcccaggcag atgctaacga ttactccatg
     7801 gattaccctt ctgagccata gaagaacaaa atataaataa atgaaaaaag ggataaaccg
     7861 catacgtcgg caaatcccta gtataagcca tcagggctga ttatttcgtt atccccagct
     7921 cctttttaac caatggacct atatcaacac tttgctccga aaagtaaagg atgaccccgg
     7981 tgctggtatc aaatatgtag gtaaacccgt tagccttggc aaccttttta attgcagaat
     8041 ctgctttttc aatgaccggt ttcataacct caccctggta gcgttgatag tcctgctcgg
     8101 ctacctgctg aaactcctga gctctttgct gcatctcggt aagctccttc tccttcatct
     8161 cgcggatggc gtcagtaaag gtgctgcgct tttgaaggta atcctgatac ttcttattaa
     8221 attcaacctg aagctcctca acctgatcct gcaacgtctt ggtaaactta tccagtttgg
     8281 caacagcgct atccctctcg ggcataacac ttagaatctc ctggctgttg atgtgcccaa
     8341 acttatagct ttgcgcatag gttttcacac cagtggctga taaaaaaaga atgaaagcaa
     8401 tcgtggatat ctttctcatg attatcaatt tttaagtaag caaatataaa aattaaatta
     8461 tttataccct aattttagga gcacatcttc acttttatcg tagcggggat tggtgtaaag
     8521 catgttgggt gatgctgcag catcgaaaat aatggcatac cccccttcga tggctatctc
     8581 cttaacggca ttaaaaacct gatcctgaat gggtttaacc agctcttccc ttttcttaaa
     8641 gagtaaccca tcgcgaccaa agtacttcat ctgcagctct ttggtttgcc tttccctgga
     8701 aattatctca tcttcacgtt ttttttgcat ttcctgggtt aaaaggacct tttcggtttg
     8761 gtagtcctta tacatcttgt caatttcagc atacttgccc tcaatctcct tttggtactg
     8821 ctccgagagc ttatcaagct gctcctgggc tgccttatag gaaggaattt tattgaggat
     8881 gtactctgaa tcgacaaaag caaatttttg tgcccacaag cttgtgctaa tcagcaagac
     8941 aattgggagt aggagtattt ttttcattgg tgtgtaattt atttaaactt aaatgcgact
     9001 actggttgcg agtaaaatta aactggtcat ttttaaatat ttaaagtatc aaaagtaatc
     9061 ggctaagtat ttcaagttga agctgaattg ttaaggatat atcaacatgc ctgccaatta
     9121 ttgaaatagg gttgatataa gggttaagaa ttgttaaaat ggcattccga taataaagtg
     9181 aaactgccct ttatgggcgt cgggattgtc gggaatttta tcgaatccgt atccccagtc
     9241 aattccaagc atccccagca tgggaaggaa cattcttgcg ccaatgcctg ccgagcggtg
     9301 cacgttgaag gggttgaact cgctggcatc acgccatgca tttccagcct caaaaaaggt
     9361 tagtaggtat atggcggcct gtgggttaag ggttaaaggg tagcggattt caacggtgta
     9421 cttgtcgtaa acatggccgt cgccaacgta gctaccatct atcagggtgt acggggtaag
     9481 agaaccattc ttgtacccac gcaggccaat tgtttccttg ccgtaaaggt tgtagcccga
     9541 cataccatca ccgcctacat caaatccctc aaagggtgaa tggccaatat cgctgttaaa
     9601 gtaacccata attccgaact ggaagttgga gtagagcacc agcttttcga ccaggggaac
     9661 ataccattga attcgcccgg tccatttatg gtattcaatc catttgtact tttcgcttcc
     9721 ggaaagtgcc gagtagttct ttccattaaa aagcgagtat ggtggggtaa tttgaagtga
     9781 caggctaagg ttggatcctg tacgggggta gataacctga tcggttgagt tgcgcgtaag
     9841 ggaaatcttg tagcttaggt tattcgactg gccgttgctg aaaagaaagt atcccgtcca
     9901 atccttcagg ttatagttct ggtagctaat ttcgttgtaa atgctgaagt agtcgtcggg
     9961 ccatttcaag cgggttccaa tacccacaga agctccggtt accttaaaat atttatcact
    10021 cgtctggtag aagtagtcca ctccgccatt ctgaatggtg tggtaaaccg agaaggaaaa
    10081 gttggttggt tttctaccac ccagccaggg ttcggtaaag gagaagctga aggctttgta
    10141 gtaggttccg ttagtagaag ctctaagtga tagggattgg ctatcacccg aaggaacagg
    10201 cctccatgcc tccttgtcaa agagtcttcg aacggaaaag ttggtaaacc ttatgccaac
    10261 cgttccaaca aacattttgt taccccatcc tccagatatc tcgagctggt cgttggcctt
    10321 ttcctccacc acgtatttaa gatcgaccgt tccctcagcg ggatttggca tgggctgcac
    10381 gtcgagtttt tcgggatcga aatgtcccat ctggccaagt tccctgatgg tgcgtgttat
    10441 ttccgatttg ctgaacaggt agcccggttt ggtccaaagc tcacggcgaa ttacgtgttc
    10501 atttgttttg gtattgccaa caatcagcac tttgttaatg gtggcctggt cgccctcgta
    10561 tattctcatc tccaggtcaa cggagtcgtt ctgaatctgg ctttcaaccg gatcgaggtt
    10621 gaaaaacagg tagccatcat ccatgtaaac ggtcgaaatg gagttgtcgt cggtgaaaag
    10681 cctcttttcc agaagggttt tatcgtaaac gtcgcccttc ttcattccca gtatggcagt
    10741 aagggcttcg gagggaagct tggtattacc aatccaggaa atgctgcgaa tgtgatactg
    10801 tggcccctcg tgaacaagaa agttaatgcc aattcgcttg ttgttcaatg taaaaacgga
    10861 atccttcaaa attttggaat cccggtaacc attctcgttg taaaatgcta tcaggttatc
    10921 cttgctctcg tcgtagtcgg actgtatgaa ttttgccgat ttaaaaatgt tgatatcacg
    10981 gcggtgaatt tttttaaggg atttacgtag gcgggcatcg gtaaatgctg tgttgccgtc
    11041 aaatgtaatt tggtcaattt tcaccctttc attttttctt atatcaaaag taagcttaac
    11101 catgttggcc aaagcggtat cattttcctg aatgggattt acatctacat tcagaaatcc
    11161 tttagtccgg tagtgcttcc tgataatctc cacgctattc tcaaggatgc tctcggttag
    11221 ctggcttccg ttgcgtagct tcaacttctc cttaaggtcg ctaacctcac cttttctcac
    11281 tccaacaaag ttgatagacg aaatacgggg ttgctcctga agaaaaatat cgaggtagat
    11341 atctgaattt tcaatttttg tagccgaaat tttaatatcg gaaaatagac cctggctcca
    11401 tagcttttta atagccttgg ttacggcatc accagggaca aggattgaat cgccaacgct
    11461 aatacccgaa actgatacca gcacatcggg attgagaaac ttgatacccg acaccctaac
    11521 ctctttgaca cagtattttt taggtacctc gtagctgaac gagggctcct gagccagagc
    11581 ggaaacgctg ctaagaagaa gtagaagtaa aaatattttt gattttagca tactgcaatt
    11641 ttgctaatta atactggggt gtaaaaataa tatattttca attgccaaag ttctatatac
    11701 aaataagagg ctgggttcaa aagtaggtaa atgaccctca gggaacaagc taaaaaatgc
    11761 gaattgagct aaagctgctc gctggtcatt ccaaaacgcc tttccctgtg ctggtactca
    11821 acaatggcat taaaaaaatc gtctttgcta aagtcgggcc agaggacagg ggtaaagtag
    11881 agctcggcgt aggctagctg ccatagtaga aaattgctaa ggcgaatttc gccgctagtt
    11941 ctgatgagta gttccggatc gggtattccc gctgtttgca ggtacccgcc aaaaacttca
    12001 ggcgataggc tcatctcgtc aagttttcca ttccggtatt cggtaaggat ttttcgtgta
    12061 gcctcggtaa tttcggctct tgccccatag tttagcgcta aaatggttgt cagggttttc
    12121 ccctgcgagg ttttatctat ggcatatttt agcttggttt gaacgttttt tggaagggcc
    12181 gatgtgtttc ctatggtaat gaggcggacg tcattcttat taagattacc tatttcatta
    12241 tcaatggttt caatcaacaa gttcatgagg gtatcaatct caattttggg tcttttccaa
    12301 ttttcggtgg aaaaagcgta aagggtaaga aactttacgc cgatttcgcc agcagcctcg
    12361 gtggctgccc taactgcacc tacaccattt ttatgcccaa aaattctggg taggccccgt
    12421 ttctcggccc agcgtccgtt tccatccata attatggcaa catgctgcgg aacgttgttc
    12481 ttatcaatct gatttttaat gtccatagta aaaaaaagtt acttatacac cgggcaaaca
    12541 ttgcctgagt tgtaaagcct ataggtgtat attagcccaa tgtacgagaa ccagtcgtta
    12601 ttgtgtagaa aaggtgtttt accatcatca ggtgcagcat agctatccag ctcatcgttg
    12661 aatgttttat ggaatcgcca ctccatcgct aacgtatgcc gtttaccggg tgaaaactta
    12721 agtcctaccc caaaagggat atggaacaaa acagacccac ttagctggcc aacgcctaac
    12781 ccaatgttta catatggaga gagtttcttt ttcctggtgc ttaaagggct aaagctaaca
    12841 aaattaaact ctcccatcac ctccgcttca agtaaggtgt tagaaaaagt agtagaggcg
    12901 gacaaaggta aaaaaaaacc ggatggtgat tctcccttaa ttgcgccata caacgctgaa
    12961 gccctaatgg agtactgcgt gttaaagttg tatcgaaaaa taacccccat ggcaaaggag
    13021 ggttggtaga gcggctttgc ctcgttgaaa tcgccatagt agtatgtgct tcccgcctga
    13081 acaccaaaat cccttttatc ctggccgtga gatttgaagg gcaacaacaa aaaaaatgaa
    13141 agaacaagta aacttgacaa aaaactgaaa aatatcctgt tagctagaat gtccattggg
    13201 gctttcttaa aattttatta ctgggaagcc tgtagtgcac tttgaagtta aaaacgtagt
    13261 agaagtcatt atattttgag ggttctggtg aaaatccgtc aaggtagtca gatagcacat
    13321 atcgtccccc cagctcaact ccaagcaaca ttctggaatg aatttgatat ttaaacccca
    13381 gaccaaaagg tactacgact gctgtatgct gtgaatcgtc aaatctatcg gaatcaacaa
    13441 ggtcattaag gggtttaacc ctataaaaac atccaccacc gccggcaaaa aggtacaggc
    13501 taaaaggttt gttaattttt cttaaacccc cgcgaacctg catgatgctg tagaagtagt
    13561 tctggtcgct ctcgggtata atgtagaact gcgcaactgc cgctaattcg gttccaaaag
    13621 tagaaaaggc gtagttgcgt gcttcattcc tagagccctt gtccgagttt ccaaaaaggc
    13681 ctaaagttaa agtaccctgg gcataaaaac gctcagttag cctgtaaccc aaccctcccg
    13741 agaaaccagg tcgtattgcc ctgatgctga tatcctttat ccccatcata ctgctaggat
    13801 cgctggtccc accaatatct ccaaagtaat gggttatggg aactcccaaa aatatttcaa
    13861 ttctatttcg tttccatacc tgggagtggc caggcagaca aagaaaaaga attatcccta
    13921 ccactataaa ggatttcttc attagccagt attatcaact acaaatatat aacaattaac
    13981 tatttaaaaa ttccattcca tgcaatccaa cagctttagc tgccattagt ttcgtttatc
    14041 cattccccaa agcaactttc ccctaagggt atgatagaag ttcgatccct ccatgctgac
    14101 aacattggca aaaaaaggag ctttcttaac ggtaacggta accggcgact tacatgttat
    14161 gcttcgggaa tccagcgata gcataacact gccatcacgt ccaacagcct cgagcagtag
    14221 gatgttgtca tcgctgatta ccaacggacg cacagtaagg ttatgaggtg caatagccga
    14281 caggattagg gcagaaaggt ttgggctaac aatgggtccg cctacgctaa gcgagtaggc
    14341 cgtggaaccg gttggagtag agacaataag cccgtcagcc cagtaggcac aaaggtactc
    14401 cccatttacg taagcgttca ccttaagaaa tcccgaacca gctttctgca tggtaaaatc
    14461 attcagccca aatggaaaag tgttaaattg ctccagcgtt gaaacaactt ccagcatgct
    14521 tctatgttca actttaaacc tccctgcaat cagcaaatca atggcattgt gcatgtcggg
    14581 aatggcaatg ttggccagaa aacctaacct accaaagtta atgcccataa taggcaagcc
    14641 agatgaccgg acaaacgaaa cagaatcaag aaacgtgcca tcacccccaa tgctgataag
    14701 tatgttagcc ttcccgttta tatcgctacc ggtattaaaa acttcaaagt caggcagctt
    14761 ggccgagaaa gtttctagga gatactccat gaagggtctg taaacccaaa cctctacatt
    14821 atgctcaacc agctggttaa aaagttcacc caaggaggga agatactccg tttcaaccgc
    14881 ttttccataa attgcaacca ccatagggtc gaattgttta gtttatatgt cgagatagcg
    14941 aaaaagaaaa ttaatcctat cgttcagcat gtcggcctca tcatcctgct taagaaacga
    15001 agctttaacg gtgtagttgt acctgttgaa cgtttgcctg atagacgtca aatcggtaac
    15061 attcaacttt agggtaagct ccatcctggt ggagttgggg tgcggggtaa cgtaggcggc
    15121 aagaattttt gcatcgtttc cctcaacaat ttggcaaacc tgagacatgg agaaatcgtt
    15181 ggcattcatt tccagcacca ctatgccccc ctgcaggctt attcccgatt ctacggcaaa
    15241 gtagtgcagc agctccatca gggtaatcac gccaaggtag ttcttaccgc tatctaaaac
    15301 gggcaccagc gatagcttca gacgcgaaag cacttccata acttcaaata tgtgctggtc
    15361 ctcaataaca taagggctat ttagcgatag gtaatggtta cctaggggtt catcgggcat
    15421 gttcaaatcg taaatgtccg attctgaaac caaccctaaa aactccttgt tgttaacaat
    15481 gggtaggtgt gaaactttga aaatatccat ccagtttaga gctgtttggc ccgtatccga
    15541 agttttaagg gcaggaataa catccgaaat catctccaga gctatcatgg taggcggctt
    15601 gaaaaaaatg gttacttttg cacaaagtta actatatcaa gattctggta tgacacgttt
    15661 gagtgtaaat ataaacaaaa ttgcaacact taggaatgcc cggggtggaa atatccccga
    15721 tgtgctaaaa gccgccatag attgccagcg ttttggagca gagggaatca ccgttcaccc
    15781 acggccagac gaaaggcaca ttcgatatca ggatgtcagg gacataaagc caatcatcac
    15841 tacagagttc aacattgaag gctatcccgt tgagtcgttc atagagctgg tactcagcgt
    15901 aaagccccaa caggtaaccc tagttcccga tccgcccgat gctataacct ccagctccgg
    15961 ttgggatacc ataaataata aggatttatt gaaacgaatg gtagcaactt ttaaacggga
    16021 gggaatacga acttccatct ttgttaatgc cgatccggaa atggtgaagc atgctgctga
    16081 taccggaaca gacagggtag aactctacac cgaaccctac gcatccatgt ttaaagtaaa
    16141 ccctgaaaaa gccatagagc catttattac tgcagccgat gctgccaagc aggtgggatt
    16201 agggcttaat gccggccatg atttgaatct tgagaaccta agctacttta acaggcatat
    16261 acccaacctg cttgaggttt cgattggtca tgccctcatt agcgatgcgc tatactacgg
    16321 actcgaaaac accattcagc tatacctgca acagcttcgc agctgatgaa acttttttac
    16381 cgggaactag gcaaaggttt tccgctcgtc atactacacg ggctttatgg ttcgtccgac
    16441 aactggatgg gaatagccaa aagattaagc acaagccata ggataatcct tcccgaccaa
    16501 cgtaaccatg ggcaaagtcc acattccagc agccatacat acccggatat ggcgaacgat
    16561 ttagccgagc tggttgacaa tttgggaatt gacaactttc acctgctggg gcactccatg
    16621 ggaggccgaa ccgccatgct ctaccagtcg ttgtttccct acatggtaaa atcgctagtt
    16681 gttgctgata tagctccttg gccataccag ccaaacgacg aggttttcag gttgtcgctt
    16741 gcagagcatt catctataat taagggtatg ctaagccttc cgcttacatc aataaagtcg
    16801 cgctacgagg ccgaaaaaat gctcacaggt agcattccca gccaaaagtt gaggcagttt
    16861 ctactaaaaa acttaaaaag agacacttcg gggagctaca gctggtgcct taacctaccg
    16921 gttctcgaaa gtagtttaag ggagctgata acgggggtaa atctggagtg cggtaagaaa
    16981 tccccaaccc gagtgctatt cgtaaaggga gaaaactcaa actatttacc ggagaatagc
    17041 gaagttcggc taaccgatta ctaccctgat tgccgcttgg taactattag aaatgcaacc
    17101 cattggcttc atgccgaaca acccgaggcg ttcacaaaaa gcatattgga atttctgcag
    17161 aaaggctaac catttaccct ctccacgttt tcgggggcaa tctgtggtaa ccctttatac
    17221 cgcatgaaaa gctgggcaac agcaagggta tccttctcgc agtaagtggc tatccggttc
    17281 agatcgttct gaagatagta acactcggca acctgactgc cgtcaatatc atcctttggt
    17341 gaaagaattc caaagatatg ggttaaaagg ttaagcgacg tgtagtgctt gttgtcgcca
    17401 aacttccaca gctccatggt atcaagaaac ctaacctccc atggtttttt gcccgacacg
    17461 tcgagtatgt cgggaattct aatgtcgtta acaagcatcc gacgggcaat atagggaaaa
    17521 tcaaattcct taccgttgtg ggcgcatagg ttaacatcct tacgcttttc aaaccggcta
    17581 agcatttcgg caaaatcggc gagtaggatt ttctcatcga taccaaaaaa tgatttaatc
    17641 ctaaagaatc gctcctctac ttttgagacg accagaccag ttgatatgca aataattttt
    17701 ccaaactcgg cataaattcc ggcccttccg taaatatcgg ctgcagtttc atgatcctta
    17761 accagctgag ctgccttttt atcccagagt ttacggtcag cttcgggcaa atcggtatag
    17821 gccggatagg ccggtaccgt ttcgatgtcg aggaaaagaa tattttctgg agaaacatct
    17881 ttaagcatgg ctacacaaga ttatgattcc taagagtctt ctcgataatg gaagcaagaa
    17941 tgctaatagc aactgcatta ttccctccct gtggcacaat gatatcggca taccgcttgg
    18001 tgggctcaat aaactgaaga tgcatgggtt taactgtttt ctcgtaccga tcgagcacct
    18061 tgtttaccga gcgcccacgc tccacaatat cccgtgcaat aacacgcgaa aggcgatcgt
    18121 cggcatcggc atcaacaaaa accttaatat ccagcaggtt gcgaagttcg gggcaggtaa
    18181 aaatcaatat accctcaatg attaccacgt ggcgtggttt aatggtgatg gtttccctgg
    18241 ctctggtgca ggttaggtag gagtaaatgg gctgctcaac tgccttgccc tcctttagca
    18301 actttacatg ttctaccagc agctcaaact caatggaatc ggggtgatca aaatttatct
    18361 cctgcctttg ctccaagggc aaatgtgagc tatcacgata gtatgcgtcc tggggcagaa
    18421 caaccacttc gcccttaggc aacaggtcta taatacggtt gacaacggtt gtttttccag
    18481 atcctgtccc gccggctatt ccaacaataa gcatacgatg gctgttaata aataatagac
    18541 aaaggtaaac taacgaagtt aaaaattttc aattaatttg taatcgaata ggaaaacttt
    18601 tatcatggag aacacgggtt tgtatccact caggtttaaa ccgatattca agcctagaat
    18661 atggggcggt aacaagcttc ggcaaaggct aaacaaaaac attcccaaga atatttcaaa
    18721 ttgtggcgag agttgggaag tatcggcagt tggcgataat atttcggtaa tagccaacgg
    18781 cttcctggag gggaacaacc tggaggagct ggttgaaata tacatggggg atctggtagg
    18841 caattcggtt tatgaaaagt ttgggataga gcttccgctc ctggtaaagt tcattgacgc
    18901 ctccgacaac ctatccatcc aggtacaccc aaacgacgag ctggcaagag aacggcacca
    18961 tgcctacggg aaaaccgaga tgtggtatgt gatagatgcc gatcctggcg ctaaaatcat
    19021 taccggattt aaccgggatg ttacacgaga ggagtacctg gagagcctat cgactgggaa
    19081 acttaccgac ctcctcaacg tggaaacagc ccttgcaggg gatgtttact acattcctgc
    19141 aggtagagtt cacgctattg ggcggggtgt tttgctactc gaaatacagc aaacatccga
    19201 tattacctat cgaatttatg attgggacag gctagacgag actgggaaac ctcgtgaact
    19261 tcacaccagc ctggctttgg atgccattga ttttagcaag gtttcaaacc ccaaaagaaa
    19321 tattactgaa agccttaaca ctcccataga gcttgccagc tgcccctact ttaccgtaaa
    19381 caagctaacc cttacccaac cccttaaaag ggattacttc atgatagact cctttgtgct
    19441 gtacatctgc ctcgcaggcg atgtcctggt aaattatccc aatggaaaag aaaaattagc
    19501 aacaggcgaa accatgctta ttcctgcaga aataaaaaat atcagcctac aacctgtttc
    19561 tacatgtaca attatcgaag tattcgttgg ataaattttt cgttggataa aatgagcgtt
    19621 ttatccaata atttatagta ttcataaatt atttacctat atttacctac caagagatga
    19681 attgaaatga agaaatttca tatcttcaac cttcattttc ctgaatggat aacatcattt
    19741 atttcataaa atctaccctg gcaagcgaaa ttgcctctag tggttttccc cttatatata
    19801 agggtgagat gaaccaccag attatgcgtt cgtttgcctt tatggccaac cgtaaaatct
    19861 ccgatatgga tgtccccacg tcaatacgta agcgggtttt ccacatcatg atagaatgtc
    19921 ttcaaaacat caccaagcac tccgacgact atgacgaaaa ggaaaggcaa ataggaaacg
    19981 ggttgtttgt ggtaggaagg gaaaaggatt cttttttcat tgtaacgggg aacctaattc
    20041 ggaacgataa aatggcaccc ctggaggaac gccttatcag cctaaacaac gcttccccca
    20101 ccgagcttaa aagtttattt cttcaacaaa tgattggtgg tgagcttacc gaaaaagggg
    20161 gcgctggatt aggtctaatt gacattgccc gcaaatcaaa gcgaaagcta tactaccact
    20221 ttgtcccata tgagaataac cgccacttct ttatcctggc cgtaaccatc cccattgaaa
    20281 ttgatgagga cgacaacgac gaaaacctat aaaccaaccc tttacagtag cttatcataa
    20341 gcgtaatccg gtaggccgat tacgacatca aaattcttgc tcaacctgca cgggataacc
    20401 atcagtataa aacttcggta tgaaaattga taaatttttg aatatctttg tggaaaatta
    20461 cttacttacc ttaactgact tctttttaag ctggtatcct ttcccaaaat tttagtcctt
    20521 ttgactcaat aatgttcata atgaaaagat atttagcaac cctctgcctg gcaacaacct
    20581 gcactatcgt actcgcccag tcacggagtg agctcatgag tcgtgagaaa attcagaaaa
    20641 acaaaattgc ctccgtttca cagtggactc ataaatttaa caaggacaag gttgacccca
    20701 aaggctacgt aaccgtagta accaactacg acaagaatgg gaatgaagtt gaaataatca
    20761 actacaagca aaacggttcc gtttcgtcgc gccacaccta caagtacgac aaccaaaacc
    20821 gtaaaataga atacctccaa taccaagact accgtcaaaa gggaattgag ctggcattca
    20881 gacaggtatt tacctacgat aagaatggga ataaagcctc agaacttggt tttgatggaa
    20941 gaaccactta ccgtattgcc tacagctatt ttcccaacgg caagcaaaaa gaaattatac
    21001 gctacaacgc tgccaaccag gtagaagaaa aatggatata cgagcataac ggtaacaaca
    21061 ccaaaatttc aatatttaaa cccgttggca ccctcagcaa aataaccgaa agaaagtacg
    21121 actccgccgg aaatctactc gacgagaaaa acacctcggg cgatgggaag gaactcgccc
    21181 gtaccgtatt tacctacaac gccaacaacc ttcccatttc caagtcggag tactacgccg
    21241 gcgagcttag ggcaagctac agctatgttt acgatggaca aaatcaactg gttgaaacct
    21301 acctgattaa accaaacaat accaggctac tctacagctc atataagtat gatgggcaag
    21361 gaaatattgt ggaggaaaaa tggtatgagg ataacgctac cgattactca caccgtaact
    21421 acaagctgga tagcaaggga atagttgacg aagttgacac ctactactcc gactataact
    21481 ttagggttgt gtaccggtac gagtacaaat tctactgacc tagctcactg attcggcatt
    21541 ccgataacca tccgccaccc tggcacagag aaaaaaattc attacggagg agagaacaac
    21601 ccaccgtcaa ggctacacta aagtgtagat ccttaaagcc tgcaacctgc cacattgcta
    21661 ctatagtgta accatacctc cgtgtaaatt tttaggtttc tgattactaa tactttttta
    21721 accataaaca cccggttgtt tatacgcgtt tattcatgct gtcctacacg ggtgttttat
    21781 cgcattaaag cttgatgcct cccggtaata aataatgctt agttttcata accattttaa
    21841 ataaagtaat atatttgaaa gtaaattcaa actgggacaa gcattaagga ggagcatggc
    21901 aaataaaagc ggtctattac acacaagtta tacactgctt ctggttctga tatttcaggt
    21961 taatgccatt tgggctcaga ccaccggaag gtttccggtg atgaactttc accataggga
    22021 gtataatggg catgctcaaa gctgggcaat aacgcaggat aaccgggggc taatatacat
    22081 tgccaacaac gttggggtta tagagtatga tgggagcgag tggcgacata tttcaattaa
    22141 tggagcacta gcacgctgcc ttgatactga caaaaacgga agaatttggg ttggtggtca
    22201 ggatgaattt ggataccttg cagccgattc gaccaatacc ctaagcttcc attccctttc
    22261 ccagcatata cccgaaacgg ccaagccgct cggaatgatt cgtcagctct acgccagcga
    22321 ttcatcaatt tacttttcat cttttactac tctattcagg ttaaacaagg atttaaccgt
    22381 ttcggaatgg tcgccaaaaa cacagtttca ccgctcctac cttaccgatg gtgtgttgtt
    22441 tatccaccag cgcgactatg ggctttgcta cctgagcaat gactccctgc gcctagttcc
    22501 gggaggagat tactttgccc cgcagcttat atacgcaata ctcccattca accaaaaata
    22561 ctttctgatt tgcactcaaa acgatggctt ttacctgctc agcaggcaag ctctagaccc
    22621 aaatttcacc ggatatacaa agcctgctat tgttaaattt tccacttctg acgataattt
    22681 tttcgcatcc tccaaggtgt atgcagggct taaactagat gataatacct ttgccatttc
    22741 aacctacagc ggtggagtgg tatttattga tactacaggc aacataacca gacgcattga
    22801 cacttcggtt ggcctacagg ataatactgc atggtatcta tacatggata accaaaaaaa
    22861 tctttggatt gcactaaaca acgggatatc ctacacaccc attttctccc agcttaccag
    22921 ctggactgaa aacgatggaa ttaagggcgt tccacaatcg gttgtccgtt ttaaaaacca
    22981 catctacttc accaccaacg ttggtatttt ctggtttagc aacggcagga tagagcaggt
    23041 tacgggttta tccgatttat catggaagct gcgaaccata cgcacaatga atggcgatga
    23101 ggccttgctt gccaccacct caagtggaat ttttcaggta gaaggaggaa aggcgacact
    23161 aattgaaaat gggaagtacc attcctactg ccttctccaa tcgaagctgt acccgaaaat
    23221 aacctacgtt gggttacgcg aaggtgtcgg tgtaatagca gaaaacaacg gacggttcaa
    23281 atttttgggg gagctgaccg gaactaaggg ggaggtttac tccctggcag aagatttaaa
    23341 cggagaccta tggtactctg aacggtacaa gggtatcaac tacgttgata tggttaaccc
    23401 ctaccagctg gtctcggaaa aacctattct ttacaccctc ccattcaatc caaggtacga
    23461 cgatattgct gtggttatga ttgatggaga agtaaaagct tctgccgagt gtggactttc
    23521 aaaattcagc caccagagta acatgtttga acccgactcc tccctagggt tggaatttgc
    23581 caatggaaca accgggttac gaatacttaa ccaggaccaa aacggtaaca tttggtttga
    23641 ggcatacaat ttttctgcaa accgatggct tgagcgcgct gttaaatatc caaagaatat
    23701 ttaccgcagg gaacctgccc aattcagaac catccccgaa actattttct acgatatttt
    23761 tcccgaagat aataataagg ttgtttggat agcttgtacc gatgggcttt tccgtttcga
    23821 tgagaacgcc ggaaatttaa aaaatccgat atttagggtt ctcattaggc aggtttcggc
    23881 cggtcctgac cacatactat ttaacggggt ttactacgat gggaatgagg ggataacatt
    23941 tcctacaagc ctcccctaca acgaagtaaa atccacaaca cttccataca aaaacaactc
    24001 cttagcattc acctactctg ctccgttctt cggtcagcag caaaaactct tatactccta
    24061 catgctggta ggatttgata aaacatggag tgattggcaa cccaaccaga gaaaggagta
    24121 caccaatctg ccctttggca cataccagtt caacgttaag gcaaaaaacc tctttaacat
    24181 cgaaagtcct gccgaaacat tccaattcac cattcaaaaa cctctctacc tccagtggtg
    24241 ggcaattact ggctatgtag ttgcactttt gctaattatc tggctatcca tcagaattaa
    24301 tacccagctg cttagaaact caaacattaa actccagcag ctggttgacg aaagaaccct
    24361 tgagctctcg aaatccgaac aggcccttat tgaaaagaac gtcgagctac aacggcaaaa
    24421 ggaggaaatc cttacccagc gggatgagct ggaggaacac aaccgccaca tcaacgatag
    24481 cattatatat gcccagacca ttcagcaggc cattcttccc gatttatccc aatcgctggg
    24541 aaatattttt gagcatttcc taatttataa gcctaaggag ctggtctcgg gcgattttta
    24601 ctggatatcc catgctggcc ctaaaaacaa cccaaacgaa aaaattttta ttgctgtggt
    24661 ggactgtacc gggcatgggg ttccgggagc attcatgtcg ttaattggca gcaggctgct
    24721 gagcgaaatt gtaaacgaga aaaaaatcta caaccctgca gatatcctca ccgagctaag
    24781 cgcttccatc aaggtggcac tccaccaaaa tgtcagcgag agcttcgacg gcatggatat
    24841 agccctttgc ttaattgaaa gcaagccaaa cgatatgttt tccgttactt ttgctggcgc
    24901 taacaggcca attttctgct atagcaaggc tgaaaacaga atacaaaccc ttaagggtaa
    24961 tagaaagtcc ataggcggcc tgctccctga tgtggatctg gaatttacaa actggagagt
    25021 tcccatgcac aaaggagaca tgattatcat gtacaccgac gggctaaccg atcagaataa
    25081 ctcctcaaaa cgcaagttta ccacggcacg gtttcaaacc taccttctcg ccaatattga
    25141 gaaacccatg gaagaaatct catccatctt gctacaggag tttaatgaat ttacaggtag
    25201 cgaaccccag cgcgacgata taaccattct gggtattcga ctttaaatcg gtgtataacc
    25261 ttgtgagaaa gctaattcta cctagtaaac tataccaaaa tagctaagca atgcaaacta
    25321 aaaagattct aatggcttta gcgctgccag cgctcgtact ggcctcatgc aaaacccagc
    25381 aaccaaataa tgaagctata gttgggcctg ttaaacctgt agtaaccgat agcaccctta
    25441 ctccagagat tctttggtcg tttggccgaa taggcgaatt tgccgtgtcg cccgatggac
    25501 agaaagttgc atacacaacc gcctggtact ctattgatca aaataggggt aactcggaga
    25561 tctacatcat gaacaccgac ggcaccggaa agcgccagct tacccataca aacggttctg
    25621 agtttaacct gcaatgggtg gatagcctta acctggccta cctcgcaacc gaaagtggta
    25681 aatcgcaaat ctggcagatg aatgttgaaa accagaaaac ctccatggtt gaaggatttg
    25741 aaaacgacat aagcggattc agcatctccc ctactggttc aaaaattctc cttacaagtg
    25801 atgtaaaggt gggagcttca attgccgata tttaccccga cctgcccaaa tcaagcgggc
    25861 aaatctacac cacgctaatg tatcgccact gggacacctg ggaggacagc ctgtttaacc
    25921 atgtcatggt ggccaacttc gatggaaaat ccttatcaaa aatcatcgac attatggcga
    25981 atgagcccta tgatacccct ctaaaaccat ttgatggtat ggaggatatt gcctggagcc
    26041 ccgatggtaa tcaaattgcc tacacatgta aaaaacttac aggaaaagag gccgcattca
    26101 gcactaactc cgacatctac ctctacgatc taagaaatgg aaagacccaa aacttaaccg
    26161 aaggattgcc gggttacgat aagcaacccg tattttcgcc cgatggggaa aaaatcgcat
    26221 ggttgtccat ggaacgagcc ggttttgagg ccgataagga tcgacttctt gtaatggacc
    26281 ttcaaacaaa aaaaacttcc tacctaaccc gtgattttga ctactctgtt tcaaacgtaa
    26341 tctggaatgc cagctcagat atggtttact ttatagcggg agttgaggca acctatcaga
    26401 tttacaggtt agccttgcag gacgctaaac ctgtagcaat tacccatggg tggcacgact
    26461 atcatgcttt tgcccttgcc ggtgataggt tgatagggag caaggcttcc atcgtaaaac
    26521 ctgtcgaaat atactccatt aaccctcaaa atggagaaga ggtggagctc tcctttacca
    26581 accaggagct gctttcccag ctaaaaatgc ccaccgttag gcaaagatgg ataaagtcaa
    26641 ccgatggaaa gctcgtgcac acttggataa ttcttcctcc cgatttcgac agcacaaaaa
    26701 gttatccagc actcctctac tgcgaaggcg gaccacaaag cgcagtaagc caattctgga
    26761 gctaccgctg gaacttttcg ataatggcct cgggtggata cgtggttgtg gcaccaagcc
    26821 gaaggggcac gcttaccttt ggacagaaat ggaccgatga aatcagcaag aatcacggtg
    26881 gtcaggatat gaaagacctt ttatcggcaa ttgacgaggt aaagaaagaa ccttgggttg
    26941 acgaaaaccg cctgggtgca gtaggggcca gctatggtgg atactctgtt ttctggctgg
    27001 caggcaacca caacaagcgc ttcaaagcgt ttattgccca ctgcggtgtt tttcattccg
    27061 aaatggagta cctcaccacc gaggagatgt tctttgacac atgggaaatg ggcggagcac
    27121 catgggaaaa gaacaatccc gtagccatga agagctacgc acaaagcccc cataacttta
    27181 taacaagctg ggatacgcca attttaatca tcagcggcgg taacgacttc agaattccct
    27241 acacacaagg aatggctgca ttcaacgctg cgcaaatact tggcgttcca agcaagttca
    27301 tatttttccc cgatgagaac cactgggtgc taaaacctca aaatggaata ctctggcaga
    27361 gggaatttag aagctggctt gacaggtggc ttaagtaggg aagttacgtt gaagtcaaac
    27421 tcgatccttt aaggctcaag aatgctgaac tcctttcatc ggttgatgaa aggagttcag
    27481 cattaaagga ctgtccaagg aatttagcta taccatggag tgttgaatgt tgtgctactg
    27541 ctctgtaagg acaacctttt gcctcagggt tggatggggg ttgacctcat ctgactcaat
    27601 cattccgtca agaattctca caatccggcg ggcatgacgg gcaatgtcct cctcatgtgt
    27661 aacaataatt atggtattac cctgcgagta gatatcctca aagaggttca tgatgtctat
    27721 ggaggttttg gagtcgaggt tgccggtagg ttcatcggcc agaatgatgg acgggttgtt
    27781 aacaagcgcc cgggcaactg ccacccgctg acgctgccca ccagataact cgttgggctt
    27841 atgctccatt ctatccccca acccaacgtt gtaaagcacc tgcttagcct tctcaacacg
    27901 ttcgtgcttt ggcactccag cgtaaataag gggtagaata acattatcga gagaggtgta
    27961 gcggggcaac aggttgaagg tctgaaaaac aaaaccgatc tccttgttgc gaatatcggc
    28021 aagctcgtcg tcgctaaggc ggcttacgtc ggttccattt agtatgtagg tcccatcggt
    28081 tggggtatct agacacccga ggatgttcat cagggttgat tttccagacc ccgaaggccc
    28141 cataatggcc acatactcgt tttggtcaat tgttaaattt atgcccttta gcgccttcac
    28201 cgtaacacta ccaacctggt agaatttttt tatttcattc agcgtgatta agttggtgct
    28261 gctcattgaa ttttgttccg ttatgttgtt ctacaaatgt ataataaata tttagggtgc
    28321 aaagtttaaa atttagtatg ggccgtaacg tagcaggaaa tgttgctttg caattccagt
    28381 cctaaagaac agtattccgc atcggaaaag gtcgatagaa atggtaacct ttgggttgct
    28441 gcatatctgc ttccatgcag cttccatgcc ggggctccat cgaatatcat caatcacaaa
    28501 aacagaagtg tcagctaccc ttggtaacat ccggttgaaa ttttggagga gcgctctgcc
    28561 attgtgatcg ccatcaataa aggcaaaatc aatagtgcta attcgtttta tgagctcggg
    28621 tagctgaaca tcaaagttgc cggcaatggg ttcgatgttg gtaaaaccca tctgcttgaa
    28681 tctattccgg gctattgcaa ggatagactc ctccccctct atggtgtaaa ccttagaatt
    28741 ggggttagct gcagccatga acatcgtgct aatgcccaca gatgttccca gctccactat
    28801 gatttcgg
//
LOCUS       DAOPHX010000016        28455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_1621_length_28455_cov_7.060000, whole genome shotgun sequence.
ACCESSION   DAOPHX010000016 DAOPHX010000000
VERSION     DAOPHX010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 28455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28455
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1621_length_28455_cov_7.060000"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..984
                     /locus_tag="PK502_02325"
     CDS             142..984
                     /locus_tag="PK502_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPG99685.1"
                     /translation="MDIENLIAVFERKLMIQRYSTSSIKNYSSSVRSFLHVAEKRFDQ
                     PDELNENEIEKYVFWKIRKDNISSSYQRMIVAAIDKFYYSVVGKNLSIRHLYPSRKKH
                     ELPKYLTRAEVKRMLQSITNPKHACIIKLLYGCGLRLNELLHLKISDIDSENMLIYIR
                     NSKGNKDRVVMLSPTLLADLRSYYKSYHPKYYLFEGQDGGMYSAKSVQTIVKTAATKA
                     GITNPVSPHILRHSFATHLLENGTGIRYIQQLLGHNSVKTTEIYTHITDVARSNIKSP
                     LDML"
     gene            1390..2193
                     /locus_tag="PK502_02330"
     CDS             1390..2193
                     /locus_tag="PK502_02330"
                     /inference="COORDINATES: protein motif:HMM:NF024671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4065 domain-containing protein"
                     /protein_id="HPG99686.1"
                     /translation="MNIKLTQKQIGHRIAELRKMKGLSQEDLAKRIKISRPSLAQIEL
                     GNRSVDILELQKLSQVLGFSLDDFMSKDFSVSQEITVKDEKKSKKMEERISVPALRIN
                     KFKNVLLYILERCAGKPNVGETVLYKLLYFSDFNYYELYEEHLTGVRYRKLPYGPVPQ
                     KLDTIINQMINQGQLQRVITEYHGYPQTRYLPLVKADLTELKASEKEVIDKVIELMSD
                     WSAAAISDYSHQDMPWLASKEGEEINYELAFYRDAPFSVRNYGDEIEEL"
     gene            2190..2534
                     /locus_tag="PK502_02335"
     CDS             2190..2534
                     /locus_tag="PK502_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99687.1"
                     /translation="MTFDELAEFKKDLKNLLKKYRTLNDDLDVVKKVLEVMPDERPPF
                     SFRIDNLDLKTCIIKVKKIACKALKGRGVNSGLRLVYAYFETEKKITFIELYHKNDKE
                     NEDRKRITDNFK"
     gene            complement(2542..3573)
                     /locus_tag="PK502_02340"
     CDS             complement(2542..3573)
                     /locus_tag="PK502_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HPG99688.1"
                     /translation="MREALESMAVIDVCSLSESVEAIQKSKGAVWCSIPDGLNKFNTW
                     LLYRRYSTKTTRTYLETLRSFLIFLQPKAATDATNNDMVRFVNDYILKNGYSLSYQNQ
                     VVNACKLYFKEIAHGELERKLPPNSQYQGDLALFLQILLQKKDSKNKIYSIHEPEVAC
                     ISKGKEHKKYEFGNKASFAKTDSGVLVAALGFRDEYDGTLKPTLEQVERLTGKTPKKA
                     KVDRGNRGNKKIGETEVLIPSTPSKSMSYYQQKKLSDSHKKRAAVEPVIGHLKTDHRL
                     NRNFYKGVVVDNHNIILAAAAFNLKRMMNGWKFYFCQILERPFLSFFKNFTTNFSLFF
                     SNKIFKLTF"
     gene            complement(3584..3757)
                     /locus_tag="PK502_02345"
     CDS             complement(3584..3757)
                     /locus_tag="PK502_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99689.1"
                     /translation="MTTELKVITCRQGTHRNNKVVFLNFEFDKTLIDAVRKLGCAKWS
                     QTDHKWCIPYREF"
     gene            4084..4683
                     /locus_tag="PK502_02350"
     CDS             4084..4683
                     /locus_tag="PK502_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HPG99690.1"
                     /translation="MAKFKPSEMDFFKFDFRQIPPKAGRLLIAQPSLTDPFFKRTVIY
                     LVEHDDEGSMGFILNRKLAMSLNDLLNNFPNVEANVSVGGPVGSDTVNFIHTFGSTIP
                     NTLDLGNGLYLNGDIDAVKALAIAGRLTRENFRIFIGYSGWGKDQLKREIDEDSWVVA
                     NFDSQLMAKGAYDSWYFAVQQLGSRFKAWTLYPENPALN"
     gene            complement(4656..5537)
                     /locus_tag="PK502_02355"
     CDS             complement(4656..5537)
                     /locus_tag="PK502_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPG99691.1"
                     /translation="MASRNYIWLNGQFFDGGNPVINFENRAFRYGDGVFETIHAFGTQ
                     PRHLPLHFRRLLQGMKILELVAPTWLTEQNLELAITKLLNKNRLFDSARVRITVYRDD
                     GGFYIPSTSNASVLIDTQPLAAKMYTLNQQGCIVGIYDEIKKPLNTLSGVKSCNALLF
                     VKAGLYCMANNLDDCIILNEQGRVAEAISSNVFVVRGGKLYTPSLAEGCIPGIMRELV
                     CTVAPTVGIEVNNQVAINPSAFDSCDEVFLTNAISGIKWVVGIGTRRYFNRVSRTLSE
                     ALNRFTFPDQFRAGFSG"
     gene            complement(5544..5972)
                     /locus_tag="PK502_02360"
     CDS             complement(5544..5972)
                     /locus_tag="PK502_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPG99692.1"
                     /translation="MEPLVVKICEGWLQSGEHRLVAIMARYRGGWIWVMNRNRSTWEL
                     PGGHIETGEEPDETASRELFEETGALDFSMVPLFDYTIQQGSARSDCRMFYAAVTKLG
                     ALPQSEIQKIGMFDSPPDELTYEGVQLQLFEAALGYVPES"
     gene            complement(5987..6604)
                     /locus_tag="PK502_02365"
     CDS             complement(5987..6604)
                     /locus_tag="PK502_02365"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPG99693.1"
                     /translation="MTYAIIIITSLVSAVALRNGELMQRLLLSPYLVFRNRQWYRLIT
                     HALVHANYLHLIVNMIVLLSFGKAVESILRQLNMEGVIGHPALHYLMLYLGGAVVATL
                     TTLKKFRNDYSYQSVGASGAVSGMIFFCIFFYPLNKLYLMGILPIPGILFAVAYLIYS
                     NYMSRKGGDNINHDAHFVGAVFGFIYPLLISPKLITVFLKQLGLM"
     gene            complement(6606..7643)
                     /gene="holA"
                     /locus_tag="PK502_02370"
     CDS             complement(6606..7643)
                     /gene="holA"
                     /locus_tag="PK502_02370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPG99694.1"
                     /translation="MAKSTTLDSYTKILEEFKNKIYKPIYLLSGEEHYYIDKLSDYLA
                     DNVLSATEKDFNLTIVYGRDVTIADVINTARRFPMMSNYQVVIVKEAQNIKNLSDLEI
                     YLKAPQPSTILVICNKLKPDGKSSSAKLTKLIGLVKQKGVYFESKKLYDYQVADWIVQ
                     YLKPKGIKIDIMAANLLNEYLGTDLSKISHELEKLIITLPAGSNQITTKSIEQNIGIS
                     KDFNRFELTKALGEKNVLKANRIADYFAHNPNANPLILTIAAIHQYFLKVFKLHFIKD
                     KTRENLARELGVNPFFVGEYQSAARKYNPKKCVEIFELLRDYDMRSKGMNNGSTDHGE
                     LLKELIFKIIH"
     gene            complement(7657..8427)
                     /locus_tag="PK502_02375"
     CDS             complement(7657..8427)
                     /locus_tag="PK502_02375"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HPG99695.1"
                     /translation="MKTKEEIVNNWLPRYTGQAVEDFGTHILLTNFRGYLDYFAKFMG
                     VSIINPDANMPCATAKGITMIDFGMGSSNAATIMDLLSAVKPKAVLFLGKCGGLKRKN
                     QVGDFILPIAAIRHEGTSDDYLPPEVPALPAFNLQRVVSSTIRNHNRDYWTGTVITTN
                     RRVWEYDDDFKDYLRKIRAMAIDMEAATIFTVGFYNEIPSGALLLVSDQPMISEGVKT
                     IESDRVVTDLYLEEQVQIGIESLTRLINNSESVKHLRF"
     gene            8878..9756
                     /gene="fabD"
                     /locus_tag="PK502_02380"
     CDS             8878..9756
                     /gene="fabD"
                     /locus_tag="PK502_02380"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPG99696.1"
                     /translation="MKAYVFPGQGAQFVGMGKDLYENSPAAREMFEKANAILGFRITD
                     LMFSGTDEDLRQTKVTQPSIFLHSVILAKVLGDEFSPNMVAGHSLGEFSALVAAGALS
                     FEDGLRLVAARAMAMQKACEKEPSTMAAVLGLDDAKVEEICRSIDEVVVPANYNCPGQ
                     LVISGSVKGVEIACQKLTEAGAKRALKLAVGGAFHSPLMEPARQELEAAIMSTPFSKP
                     ICPVYQNVTAMPTTNPDEIRKNLIAQLTSPVRWTQTVQNMIADGATSFVELGPGNVLQ
                     GLIKKIDRSVVAESRQ"
     gene            complement(9913..10326)
                     /locus_tag="PK502_02385"
     CDS             complement(9913..10326)
                     /locus_tag="PK502_02385"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPG99697.1"
                     /translation="MSTTSTIIYQGNLRTVATHTRSGEQISTDAPVDNQGKGEFFSPT
                     DLLATSLGACMVTIMGISAQTHGFNIDGTRLEVQKIMGTNPRRVVEVIIDLYFPSDYS
                     DKHKRIIEAASRECPVAQSLHPDLKQTIRFHYAEN"
     gene            complement(10426..11763)
                     /gene="gdhA"
                     /locus_tag="PK502_02390"
     CDS             complement(10426..11763)
                     /gene="gdhA"
                     /locus_tag="PK502_02390"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPG99698.1"
                     /translation="MEVSKLMAELEKKHPGEVEYLQAVREVLESIEEVYNQNPQFESA
                     KIIERLVEPDRIFTFKVPWTDDEGKIHVNLGYRVQFNNAIGPYKGGLRFHPSVNLSIL
                     KFLGFEQIFKNSLTTLPMGGGKGGSDFNPKGRSDAEIMRFCQSFMLELWKYIGPETDV
                     PAGDIGVGGREIGFLYGMYKKLAHENTGVLTGKGLNWGGSLIRPEATGFGAIYFVQEM
                     LATRNIKLNGKTVALSGFGNVAWGAALKATELGAKVVTISGPDGYIYDEAGVSGEKIN
                     YMLELRNSNQDIVAPYAKKFPGSKFVAGKKPWEVKVDIALPCATQNELNGDDAALIIK
                     NGSICVAEVSNMGCTPEAVEAFTKAKFLFAPGKAVNAGGVATSGLEMTQNSMKLNWPK
                     EEVDVRLHQIMKDIHNACVKNGTGKDGYVNYVKGANIAGFLKVANAMLDQGLV"
     gene            complement(11932..14925)
                     /locus_tag="PK502_02395"
     CDS             complement(11932..14925)
                     /locus_tag="PK502_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPG99699.1"
                     /translation="MSEENSEQSYLNSYQLDVYDTSFDKLMQKRIYKVLIICSSYDAF
                     MLEEDGRIDEQIFNEYVSLNLRYPPVFIQANTAREALRVLQEEKIDLIISMLNVGEID
                     PFRLAKIFKGKYPNVPIVLLTPFSREVSLQIQKEDLSSIDYVFCWLGNADIMLAIIKL
                     IEDKMNADHDILEIGVQAIILVEDSIRYYSSFLPNIYKIVFRQSRSFMKEALNEHHRM
                     LRMRGRPKILMATNYNDAIALYNRYRNNVLGIISDISYKVDNKRDHLAKSGLRLCRVI
                     RENDPNIPFLLQSSDRNNKVYADEMGVGFLHKYSKTFSIDLRNYMINNFGFGDFVFRD
                     PDTLAEVARASDLSQLQQLILTVPDRVLVYHTSRNEISRWLYARAIFTIAELFKPMTL
                     KDFNSIDDVRSFLYKAISAYRLSKGRSLIANFDRNTFDEYKFFTRIGEGSIGGKARGL
                     AFINQVIRKHRLFNKYEGIIISIPRTVVVSTEIFDEFMESNNLYDTALQDISDENVLK
                     AFLNARLPERLKVDLRKVISVFTRPLAVRSSSKLEDSHYQPFAGIYSTYMVPRSSDSE
                     KMLYMVCQAIKAVYASVFYRSSKAYMNATQNVIDEEKMGIVIQEICGNEYGSWYLPTL
                     SGVARSINFYPVGSEQPFDGIASIGFGLGKLIVDGGVALRFSPKYPKKILQLASPETA
                     IRQTQKYFYALNTDPDTFEPMVDDGMNLLKLEIKDAYSIPDLRHTVSTYDMQSGMLRD
                     GFYDEGLKLVTFAPILKHGIFPLAEILTDLLSIGQKEMNNHIEIEFALNMSPRGGTPK
                     MFNFLQIRPIVDTDQSEKFNWEAVDFSKAIIFSNSALGHGLVKNIQDFVYVKPDRFNP
                     AKTIEIAREVEEINNKFIELQRNYVLVGPGRWGSSDPWLGIPIKWSQISEARVIVESG
                     LDSFRVDPSQGTHFFQNLTSFGVAYLTINPFLNDGIYDIEFLNAQPAFLETTFCRWVN
                     FSKPLTIQVDAKNNKGIILKSNGNS"
     gene            complement(15157..15579)
                     /locus_tag="PK502_02400"
     CDS             complement(15157..15579)
                     /locus_tag="PK502_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPG99700.1"
                     /translation="MMRRRSTLPSIVDEFFGDDLWNRFFDDTESVTVPSVNIKEGKDE
                     YAIEVAAPGFDKKDFRIDLNNNVLEISSEKEVKEENKDEKVMRREFRYSSFKRTFTLP
                     DTVDTEKIKASYKDGILSISVPKKDEAKVKPTKQIAIS"
     gene            complement(15872..16552)
                     /locus_tag="PK502_02405"
     CDS             complement(15872..16552)
                     /locus_tag="PK502_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPG99701.1"
                     /translation="MKRLLFISLLLATAVVDVKAQYSRASLWTSDMERFAREDSTHGC
                     KANCVLFVGSSTFTRWTTAVADFPESNILNRAFGGSTLTDLIYYFDQIVLPYNPLQVV
                     IYEGDNDLAETSKSVGEFIDDVTTITRLVNIHFPRAQILLVSIKLSPSRKEYFSKYLK
                     ANELMKQMADSSEYIDFVDTCTPMLNPDGSPNEDFFGSDLLHLNEKGYLLWKSLLYPR
                     LMKAGQKE"
     gene            16889..20503
                     /locus_tag="PK502_02410"
     CDS             16889..20503
                     /locus_tag="PK502_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPG99702.1"
                     /translation="MVEKASKTKSIATDSALGEEQGVVEFDKLKSLVLQNESSIYVVQ
                     SFRVVFCNQSFSDLTGYTEQEVKEILFLDIVHPKDRKLIKILFQNDFQEIRQHKSNSF
                     TLRILTRDNELRWIKSHISLIAWEGKSALLCTSYDITQQKEAEENLADLEQNFGILAN
                     TFGDFIFVLNHNHNIIQANRAVIDRLGYQEHELLLESFVNLHAPSDRSTVRSLLLGVF
                     AGTHCSYSSFLLAKSGYRIPVEVRMVKGMWKRKASIFAIAQDITPRIEAEREVKASEE
                     KFSKAFNTGAVMMAICTMDGGTYLDVNEAFLQKTGLKRDEVIGQSLHDQKIYSDVNRL
                     NQLLAQIREKGKVNNVEVGISNQRGEELTLLLSAEVISIQENDCIMIAMNDITERKRM
                     EEEITNSRIQLKGILDNLPFIAWLADNKGRYLLVNKSFTSFFNTDEGRVLGKKHGDFW
                     AKAHSANLRKHEVEAAKAKKSLSWEAFEKNDLQEEWWEHFVTPVINAENEVIATTGLA
                     RIITDQKLIRLQIEQNLERQKFLTEISYLFNTGVKFETKLNQSLQKVSGLLKLKRAFI
                     VERIHSTTPSPKVFEWHDSTANLDQDALISSLDKYSPAIFDHLNQTSQLVADICSTEE
                     PSVNSLSKALSAKNLFIYPLISEGCCIGFIGVEYSSYNPQTTGNNREFMLTFSSILTA
                     ALEGQSKESKLLESEQRFRTFSELLPEMVFEANDRQVIVFTNNNLLEAFGFSKRKLPA
                     SLKIHDLFMASEHDKLTNHLAKSAQNGQQQSAELLAQRRDKTTFPTLTHVRRVKLSKS
                     KYRYIGVMVDITMQKQQEAELLKAKEQAEAAGRAKERFLSTMSHEIRTPMNAIIGMTN
                     ILLEENPATSQLVNLNALKFSAENLLTLLNDILDFSKIEAERVDLLINETDIHSLHAG
                     ILSSYAQLAAQKGIKLTGSISPLIPHKLLADRVRLNQILTNLVGNAIKFTSNGGVDLS
                     ISLQRQLKKSVWVKFTVTDTGIGIPKDKQKKIFTEFTQVHDQGGKYGGTGLGLAISNR
                     LVNLMGGQIRLKSVFGKGSTFYFSIKLDLEPYHETPEPAEAPEIKLDPTRSYLVLVVE
                     DNELNTLIVQRFLTGWGINNHHAANGVVALEMAKNNRYDLVLMDLEMPEMNGYEATKA
                     LRRLKGKTNNSVPIIALSASAMLDVKRKIFSIGMNDFILKPFKPADLKKKVLFYLSKV
                     "
     gene            20511..21893
                     /locus_tag="PK502_02415"
     CDS             20511..21893
                     /locus_tag="PK502_02415"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPG99703.1"
                     /translation="MRRFIVFFAAVSSLSFFPLEKNAAQVTIELRVKESIGSTSKLSV
                     YTGKEYVTVDSCRPTIEGIYRFNLPETAPLGLYKLTVGKSGSFDLIVSGEKHVALQTV
                     AFAVEDSLRVIDSDENKVFFDYIKLKKTSEQQHWLIKSLLQYHTSGDDVYEKLVDEQR
                     RLTDSFDSRASELAQAYPSLFVSSYINLDTKLQATGTKSDCNSRRMKADIWWNGVNLL
                     DNRLLNTPILIPRLWSYIESLLCEDELGMEEQDSIFVHYIQKLFALPLSEEIRNKFAE
                     TLADGFAETDYFEVIDYLAHAGGKAAATVFDNPDFKTRLELERPLMVGSKAHDFTAKL
                     MEGEKFKLSRSVSRYELIVFWSVWCPHCVETLPKIRSIYEEFRHKGFDVVAVNIDEEQ
                     EPWKEFTSNNSLQWHNTQVPFSSNSPIPEYYNVDGTPKMFLVDNSLRILSRPTSAAQL
                     HATLRKLYGE"
     gene            complement(21998..23890)
                     /gene="speA"
                     /locus_tag="PK502_02420"
     CDS             complement(21998..23890)
                     /gene="speA"
                     /locus_tag="PK502_02420"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HPG99704.1"
                     /translation="MRKWRIEDSAELYNINGWGVNYFSINSNGNVVVTPKNNGVEIDL
                     RELVDELQVRDVSAPMLIRFTDILDNRIEDMSRSFEVAAQEYGYKGQNFIIYPIKVNQ
                     MRPVVEEIINHGKKFNIGLEAGSKPELHAVIASNPDNESLIICNGYKDEDYIELALLA
                     QKMGKKIFIVIEKQNELKLVARVSQRLKIKPNLGIRIKLASVGSGKWEDSGGDVSKFG
                     LTSSELIQALEFLEGNGMKDSLKLVHFHIGSQVTKIRRIKLALREASQFYVQLYLQGF
                     NIEFVDIGGGLGVDYDGTRSSNSESSINYSLQEYVNDSVSTIVDVSDKNNIPHPNIIT
                     ESGRSLTAHHSVLIFDVLETASLPSWEENEDIGENPHELVSELYEIWDKLNQSKMLES
                     WHDAQQIREEALDLFSLGLLDLKTRAQVERLFWSIARDINQMTADIKHVPDEFRSLPK
                     LLADKYFCNFSLFQSLPDTWAIDQIFPIMPIQRLNEKPDRAATLQDVTCDSDGKIDNF
                     ISTRNISYYLPVHTVKPNESYYIGAFLVGAYQEILGDLHNLFGDTNAVHVSVNDKGYE
                     IDQIIDGETVAEVLEYVQYNPKKMVRTLETWVTTSVKAGIISAKEGKEFLSNYRSGLY
                     GYTYLE"
     gene            complement(24218..25132)
                     /locus_tag="PK502_02425"
     CDS             complement(24218..25132)
                     /locus_tag="PK502_02425"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HPG99705.1"
                     /translation="MRTKAISMIHGRHARLTRSAILFLALTCIGKVAGAQENIKPAIP
                     AKDFTTRIWLGSQLLPGYGQIANRQYWKLPVLYGGMGSMIYLGVKANQTYLSRLSAYN
                     SLTSTDPNRELYKERMVKQRQTRNLYIAAAGAFYVAGAMDAVLVYNNSKHSPAAATIL
                     STLVPGMGQAYNQKYWKIPVVYGGLATLYYLVSWNDRYYSRFKTALKLNIDGDDTTVD
                     EFGGARTADELRYYMDSYRRNRDLSILGLTAIYIINILDANVDGFLHDWNVDDNLSFR
                     VEPAIVNPALTATADTHSALGLSCRVTF"
     gene            complement(25170..26075)
                     /locus_tag="PK502_02430"
     CDS             complement(25170..26075)
                     /locus_tag="PK502_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPG99706.1"
                     /translation="MTKKMALGRGLGALIEDSTFESTRNSDVQTPRHAISELVQEVDI
                     ELIDPNPFQPRTNFDDESLSELAESISKLGIIQPITVRLLDGRYQLISGERRFRAAKL
                     AGLKTLPAFIRTADDQGMLEMSLVENIQREDLNAIEVAISYQRLIDECSLTQDALSER
                     VGKKRATISNYLRLLKLPAEIQLGIINSIISMGHARALISIDDASLQLKLYKKVISDD
                     LSVRQTEELVRNTVSEPQIKQSRSKVMPDLAGPELALREHLTNRLGLRADVKPGGKGD
                     GKIVISYNNPDELSVIIDKFTKIEE"
     gene            complement(26106..26909)
                     /locus_tag="PK502_02435"
     CDS             complement(26106..26909)
                     /locus_tag="PK502_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG99707.1"
                     /translation="MGKVIAIANQKGGVGKTTSAINLSASLAVLEKKVLLIDADPQAN
                     ATSGTGFDIRNIKTSIYECLIDEVNPNDIILNSEIKGLDLIPSHIDLVGAEIEMLNLP
                     NREKILKEVVAKVKDSYDFIFIDCSPSLGLITVNALTAADSVMIPVQCEYFALEGLGK
                     LLNTIKIIQSRLNPELQIEGFLLTMYDPRLRLSNQVVEEVRKHFQQMVFDTIIQRNIK
                     LSEAPSYGKPVILYDAASTGSANYINLARELLQKNNQTQIENEQKIAQD"
     gene            27157..28146
                     /locus_tag="PK502_02440"
     CDS             27157..28146
                     /locus_tag="PK502_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HPG99708.1"
                     /translation="MVNSWFVIVNPRAGGGKGEVDWPLIKGLLTKEGIAFGFALTEHK
                     HHAVELTVKAINSGYRQLIAVGGDGTLNEVVNGIFIQQSVPPAEILVGVIGVGTGNDW
                     FRMYEIPYRYADSVRAIKNRKVFYQDVGVVEYYESSVKHKRYFVNTAGIGFDAEVALR
                     TNRLKDLGRHGFLLYLISILRALFSYKTTRINVNVDGRHLQDKIFSITLGICRYNGAG
                     MLQVPFAISDDGMLDVTVIRRISKLDALTSIPRLYNGKILKHSRVTGLRGQIVEIMSI
                     PSTNLEADGESLGETPLRFTVLTRAIGVVVDENFDAKDNRPAVIQEKQTLSAV"
BASE COUNT         7783 a         6466 c         6500 g         7706 t
ORIGIN      
        1 tgcgtaaacc actttcgagc ttatgcttga tatttagtgt taatttacat gatatcaata
       61 tgttaaacta ttattttttt aataaaaata tttgcattta cgcatgtttg gattaatttt
      121 gctatggtta aatctgaata aatggacatc gaaaatttaa ttgcggtttt tgagcggaaa
      181 ctcatgatcc aaaggtacag caccagttcc atcaagaatt acagcagttc ggtgaggagt
      241 ttcctgcatg tggcggaaaa gcggtttgat caacccgacg aattaaatga aaatgaaatt
      301 gaaaaatacg ttttctggaa aataaggaaa gacaatataa gcagttccta tcagcgtatg
      361 atcgtagctg ctatcgataa attttactac tcggtagtag gtaaaaattt gagtatacga
      421 cacctctatc catccaggaa aaaacatgaa ttaccaaaat acctgacccg cgcggaagtg
      481 aaacggatgt tacaatctat caccaatccg aaacatgcct gcatcatcaa gctgctttat
      541 ggttgcgggt tgcgtttgaa tgagctgctt catttaaaaa tttcggatat cgattcggaa
      601 aacatgctga tttatattcg caattccaaa gggaataaag acagggtggt aatgctgtcc
      661 ccaactttat tggccgattt gagaagttat tacaaatcct atcatccgaa atactatcta
      721 tttgagggac aggacggtgg aatgtattct gcgaaaagtg ttcaaaccat cgtgaaaacg
      781 gctgcaacga aagcaggaat tacaaatccg gtttcacctc atatcctgcg gcatagtttt
      841 gctacccatc ttctcgaaaa cggaacaggt atcaggtaca ttcagcagct tctgggccac
      901 aactctgtta aaaccaccga aatctacacg catataaccg atgttgccag atctaacatc
      961 aaaagtccgc tggatatgtt ataaccaatc atccaatttc tgtccagtgt gatttgcaat
     1021 ctggtttcgt gattcatgga ttccatgtct gcaactcatc ccgcttcgga ttataaatcc
     1081 tcgtgtctgg gtatcagatc agaaaaatat cacgaaaata tcacggctat tcaccaatgc
     1141 aaaaaattat gtcaatagtt gttcgtttag tcgtttaatt gttctttact ttgtttattt
     1201 ttttggggtt ctgttatcta tttctttttc cctgcgtatt tgctggtggg gttttggcaa
     1261 tgtgcttgca aatgcgttgg gctttcatag gacgaatata aaaaaatgtt tttatttaga
     1321 actatgaatt gttttgtaat gaaacatggt ttatctttgt gacgttaatc gtcgaaacat
     1381 aaaattgata tgaatataaa attgacgcaa aagcaaattg ggcataggat agctgaactt
     1441 cggaagatga aggggctgtc tcaggaagat ttggctaaaa gaatcaagat ttcccggccg
     1501 tcattggctc aaattgagct aggcaatagg agtgttgata ttcttgaact gcaaaaactt
     1561 tcacaggttt tgggcttctc tttagatgat tttatgtcta aagatttttc cgtaagtcag
     1621 gaaataaccg taaaagacga gaaaaagtca aagaaaatgg aagagcgtat ttcagttcct
     1681 gcgcttcgga tcaataaatt taaaaatgtt ttgttgtata ttcttgagcg ttgtgcaggc
     1741 aagccaaatg ttggagaaac agttctctat aaactgcttt atttctccga cttcaattat
     1801 tatgagttgt atgaagaaca tttgacaggg gtgagatatc gcaaactgcc ttatggacct
     1861 gtcccgcaaa agttagatac aattatcaac caaatgatta atcaggggca gttgcaacga
     1921 gtgataacag aatatcacgg ttatccgcaa acccgttact tgcccttagt aaaagcggac
     1981 ctaacggagc ttaaggcaag cgaaaaagaa gtaattgaca aggttattga gctaatgagt
     2041 gattggtcgg cggctgcaat tagcgactat tctcatcagg atatgccttg gttggcttca
     2101 aaagaaggag aagaaataaa ttatgaattg gctttttata gagatgctcc tttctctgtg
     2161 agaaactacg gcgatgaaat tgaagaacta tgacgtttga cgaattagcc gaatttaaaa
     2221 aggacttaaa aaaccttttg aaaaaataca gaacgctaaa tgacgattta gatgtggtta
     2281 aaaaggtgtt ggaagtaatg cctgatgaaa ggccaccgtt tagttttcgt attgataatt
     2341 tggatttgaa aacgtgtatt attaaagtga aaaagattgc ttgcaaagca ctgaaaggca
     2401 gaggagttaa ttcgggattg aggttggttt atgcttattt tgagacagag aaaaaaatta
     2461 ccttcattga attgtatcat aagaacgata aggagaatga ggataggaaa agaataacgg
     2521 ataattttaa atagtcgtcc cttaaaaagt caatttgaat attttatttg aaaaaaataa
     2581 agaaaaattg gtagtaaagt ttttgaaaaa agataaaaat ggcctttcta aaatttgaca
     2641 aaaataaaat ttccacccgt tcatcatcct tttgaggtta aaagcggcgg cagcgagtat
     2701 aatattgtgg ttatcgacca ctactccctt gtaaaagttg cgatttagcc tgtgatccgt
     2761 ctttaagtgc ccaataaccg gttctacggc agctcgtttt ttatgactat cgcttagttt
     2821 tttttgctgg tagtagctca ttgatttact tggcgttgaa ggaattaata cctctgtttc
     2881 tcctattttt ttattccctc tgttccccct gtccactttt gcttttttgg gtgttttgcc
     2941 ggttaatcgt tctacttgtt ccaatgttgg ttttagtgta ccgtcgtact catccctaaa
     3001 gccaagggct gctaccagca ccccgctgtc ggttttagca aacgaggcct tgttgccaaa
     3061 ttcatacttc ttgtgttctt taccttttga aatacacgct acttcgggtt cgtggatgga
     3121 ataaatttta tttttactgt cctttttctg caatagaatt tgtaaaaata aggctaaatc
     3181 accctggtac tggctatttg gtggaagctt tcgctccagc tcgccatgcg caatttcctt
     3241 aaagtaaagt ttgcacgcat ttacaacctg attctgatac gagaggctgt acccgttttt
     3301 taaaatgtaa tcattaacaa agcgcaccat gtcgttattg gttgcatcgg tcgctgcttt
     3361 gggttgaagg aatattagaa aactccttag cgtttcaagg taggttctgg ttgtttttgt
     3421 gctataccgc ctgtataaaa gccatgtgtt gaatttattc aacccgtcag gaatgctgca
     3481 ccaaacggca ccctttgatt tctgaatagc ctccacactt tcgcttaatg agcaaacatc
     3541 tataacggcc atactttcaa gcgcctccct caaccagtca atgttaaaac tccctgtagg
     3601 gaatacacca tttatggtct gtctggctcc atttggcaca gccaagcttc cttacagcgt
     3661 caatcagcgt tttgtcaaac tcgaaattta ggaacactac cttgttgttc ctatgcgtgc
     3721 cctgcctgca ggtgataacc ttgagttcgg ttgtcatgtt tcaagttgct gattggccaa
     3781 cagtggtaaa actttcttat ttaggtcaaa ctaataaaaa tattcaatgg atacagctaa
     3841 atggggtgca ttttcaatgt agattgctcg taattcttca ttttatgtag ctgccattcg
     3901 gccaagttat acatataccc cctcggcctg ctcggtttcc agctccaacc ccacccctgc
     3961 ctgtaaagct aaagttacgt aaattttgac aggtagcgga taaccttcaa ggatttggca
     4021 taattttaga agataatagg cggtttttct cacgtaaccc taaagccgga aaggaggtgc
     4081 accatggcaa agtttaaacc atcggaaatg gatttcttca agttcgactt caggcagatt
     4141 ccgccaaagg ccgggcggtt gcttattgcc cagccctcgc ttaccgatcc gttctttaag
     4201 cggacagtga tatacctggt ggagcacgac gacgaggggt ccatgggctt catcctcaac
     4261 cgcaagctgg ccatgagctt gaacgacctg ctgaacaatt tccccaacgt ggaggccaac
     4321 gtctcggtgg gcggccctgt gggctcggat accgtgaact ttatccacac gttcggcagt
     4381 accattccca acacgctcga cctgggcaac gggctctacc tcaacggcga tattgacgct
     4441 gttaaggcct tggccattgc cggtaggctc acccgggaaa atttcaggat attcatcggc
     4501 tactccggat ggggcaagga tcagctaaaa agggagatcg acgaggattc atgggttgtt
     4561 gccaacttcg actctcagct tatggcgaaa ggagcttatg atagctggta ctttgccgtt
     4621 cagcagctgg ggtcaaggtt taaggcgtgg accctctacc ccgaaaatcc agccctaaac
     4681 tgatcgggaa aggtgaagcg gtttagtgcc tcgcttagcg ttctactcac ccggttgaag
     4741 taccttcggg ttccgatgcc taccacccat ttaatccccg atatggcatt ggtgaggaaa
     4801 acctcgtcgc agctgtcgaa tgccgacggg ttgatggcaa cctggttgtt aacctctatt
     4861 ccaacagttg gggcaacagt gcacaccagc tcccgcatga tacccggtat gcacccctca
     4921 gccagcgagg gggtgtagag ctttccgcct cttaccacga acacgttgga cgaaatagcc
     4981 tcggcaaccc ttccctgctc attcaggatg atgcaatcgt ccaggttgtt ggccatgcag
     5041 tatagcccgg ccttaacaaa aagtagcgca ttgcagctct taacgcccga aagcgtattc
     5101 agcggttttt tgatctcatc gtaaattcca actatgcagc cctgctggtt tagggtgtac
     5161 attttggctg ccaacggctg tgtgtcaata agaacgcttg cgttggaggt agaagggata
     5221 tagaatcccc catcgtcgcg gtaaaccgta atcctcaccc gtgcactgtc aaaaagtcgg
     5281 tttttgttaa gcagcttcgt aatggctagt tcaaggtttt gctcggtaag ccaagttggt
     5341 gcaacgagct ccaggatttt cattccctgc agcaggcgcc tgaagtgcag gggaaggtga
     5401 cgtggttgcg ttccaaaggc gtgaatggtt tcaaaaaccc catcaccata gcgaaacgcc
     5461 ctgttctcaa aattaatgac agggttaccg ccgtcgaaaa actggccatt aagccaaatg
     5521 tagttcctgc ttgccattcg gcattaactt tcaggtacat acccaagcgc agcctcgaaa
     5581 agctgtagct gaaccccctc gtatgtcaac tcgtctggag ggctatcaaa cattcctatt
     5641 ttctgaattt cactctgggg aagagcgcca agcttggtta ccgcggcgta aaacatccgg
     5701 caatcacttc tggcgcttcc ctgctggatg gtgtagtcga aaagcggaac catgctgaaa
     5761 tccaaggctc cagtctcctc aaacagctcc ctgctggcgg tttcatctgg ttcttctccg
     5821 gtttcaatat gtcctcctgg cagctcccag gttgagcggt tacggttcat cacccaaatc
     5881 caccctccac ggtatctggc catgatggca acaagccggt gttccccact ctgtagccat
     5941 ccttcacata ttttcacaac caagggttcc atataccggc atcaagctac attagtccaa
     6001 gctgctttaa aaaaacggta atcagtttag ggctgatgag cagcggatag ataaagccaa
     6061 atacagcacc tacgaagtga gcatcgtggt tgatgttatc gccacccttt cggctcatgt
     6121 agttggagta aatcaggtag gcaacggcaa acagaatacc aggaattggc agaatgccca
     6181 tgaggtagag cttgttaagc gggtagaaaa aaatgcagaa gaatatcatt cccgacacag
     6241 cccccgaagc gccaacgctc tggtaggagt aatcgtttct gaactttttg agggttgtaa
     6301 gggtggcaac aactgcacca cccaggtaga gcattaggta gtgcagggca ggatgaccga
     6361 taaccccctc catgttcagc tgccttagga ttgactccac cgccttccca aacgacagca
     6421 gcacaatcat gttgacaatc aggtgcaggt agtttgcgtg aactaaagcg tgggtaatca
     6481 accggtacca ctgcctgttc cggaacacca ggtagggact caggagcaat cgctgcatca
     6541 actccccgtt tcggagcgca acagccgata ccagcgaggt gatgatgata atggcgtagg
     6601 tcatgctagt gaattatctt aaaaattaac tccttcagca actcaccatg gtcggttgat
     6661 ccattgttca ttccttttga acgcatgtcg tagtcgcgga gtagctcgaa tatttcaaca
     6721 cacttctttg gattgtactt tcgggcagcg ctttggtact cgcccacaaa aaaaggatta
     6781 acgcccagct ccctggccaa attctctcgt gtcttatcct taatgaagtg cagcttaaag
     6841 actttcagga agtactggtg aatggcagca attgttaaaa tcaaagggtt tgcattgggg
     6901 ttatgggcaa agtaatcggc aatacggttg gccttcagca cattcttctc acccagtgcc
     6961 ttggtcagct caaagcggtt aaagtccttg ctaataccaa tgttctgctc aatacttttg
     7021 gtggtaatct ggttgcttcc ggcaggcagg gtaatgatta gcttctccag ctcgtgggaa
     7081 attttgctta gatcggtacc caggtactcg ttgagcaggt tggccgccat gatgtcaatt
     7141 ttaatgcctt tgggtttcag gtactggaca atccagtcgg ccacctggta atcgtaaagt
     7201 tttttcgact caaagtacac ccctttctgc ttaaccaatc caatgagttt ggttagcttg
     7261 gcagagctgc ttttcccatc gggcttaagc ttgttgcaga ttaccagaat agttgagggc
     7321 tggggtgctt ttaggtaaat ttccagatcg cttaggttct taatgttctg ggcttccttc
     7381 actatcacca cctgatagtt ggacatcatc ggaaatcgcc tggcggtgtt gatgacatcg
     7441 gcaatggtaa cgtccctgcc gtacacaatg gtaaggttaa aatctttttc ggttgcggat
     7501 agcacgttgt cggcaaggta gtcggataac ttgtcgatgt agtagtgctc ttcgcccgat
     7561 aggaggtata tgggcttata aattttattt ttaaactcct ccagaatttt agtgtaggaa
     7621 tcgagggtgg tgcttttagc cattgccgga caaaggttag aaacgaagat gctttacgga
     7681 ttcgctgttg ttgattaagc gggtaagtga ttcaatgccg atttgaacct gctcttcaag
     7741 gtaaaggtcg gtaaccaccc tgtcgctctc gatagtttta accccctcgg aaatcatggg
     7801 ctggtccgaa acgagcagca gggcacccga aggaatctcg ttgtaaaagc ccaccgtaaa
     7861 tatggttgct gcctccatgt cgatggccat ggctctaatt ttccgaaggt aatccttaaa
     7921 atcatcgtcg tactcccaaa ccctacggtt ggtggtaata accgtgcctg tccagtagtc
     7981 gcggttgtgg ttgcgaatgg tcgaggaaac cacccgctga aggttgaatg caggcagggc
     8041 aggcacttcg gggggcaggt agtcgtcgga tgtgccctcg tgcctgatgg cggcaatagg
     8101 taggatgaag tcgccaacct ggttcttcct tttcaacccg ccgcattttc caaggaaaag
     8161 aacggccttt ggcttaacag cgctaagcag atccatgatg gttgctgcgt ttgaactccc
     8221 catgccaaag tcaatcatgg ttatcccctt tgcggtggca cagggcatgt tggcatcagg
     8281 atttattatt gaaaccccca taaatttggc aaaataatcc aaataacccc tgaagttggt
     8341 caaaagaata tgggtaccaa aatcctcaac cgcctggcca gtatagcggg gcaaccagtt
     8401 atttacaatt tcctccttgg tcttcattcc gtaatcgtta ttagggttaa aagtatgttg
     8461 atggagcgct actcatttag gcctcttcat ttcggctttc atgcaaaagt agtttaaaaa
     8521 ttttattggg gcagcataac ggcattatta ccatgaagca atgtttttta accatttaca
     8581 ggtttaccca tgcggctaac ttgacaatct gaaaaaatgc cctacttttg tcagccggat
     8641 ggatgggtca tggccgggaa aattttccca gggggattta tttttgcttg acaaaaatag
     8701 agtcataata ccttccgctt aactttttta gaggattaat gtaaaacaag cggcaaaacg
     8761 aaccaataaa atttaccgat tacaagcgtt tttgttggtt ttggcttcac ataaaactca
     8821 aaagactaag tattgaccaa cgtaaaagtg taaacacaaa cctaaaattt tctacggatg
     8881 aaagcgtacg tatttccggg acaaggcgcc cagtttgtag ggatgggaaa agatctctac
     8941 gagaactctc cagcagcaag ggaaatgttc gagaaggcca acgccatact aggctttcgc
     9001 attaccgacc taatgtttag tggaaccgac gaggatctaa gacaaacgaa ggttacccag
     9061 ccctccattt tcctacactc ggtaattctg gctaaagttt taggcgatga atttagcccc
     9121 aacatggttg ccggtcattc acttggcgaa ttttcggccc tggtagctgc cggtgcgctg
     9181 agctttgaag atgggctaag gctggtggct gcacgcgcca tggccatgca gaaagcttgc
     9241 gaaaaggaac cctcgaccat ggcggcagtg cttgggctgg atgatgcgaa ggtggaagaa
     9301 atttgccgct ctattgacga ggttgttgtg ccggctaact acaactgccc cggacagctg
     9361 gttatttccg gatcggtaaa gggggttgaa attgcctgcc agaagcttac cgaagcgggt
     9421 gctaaacggg cacttaagct ggcagtgggt ggtgcattcc attctcccct tatggagccg
     9481 gcccggcagg agctcgaagc ggccatcatg agcactccgt ttagcaaacc gatatgccct
     9541 gtttaccaaa atgttacagc aatgcctaca accaaccctg atgaaatcag gaaaaacctg
     9601 attgcccagc taacctcccc ggtaagatgg acacaaaccg tccagaacat gattgccgat
     9661 ggggctacat cattcgttga gctgggacca ggcaacgtgc ttcaggggtt gatcaagaaa
     9721 attgaccgca gcgtggtggc cgaaagcagg cagtaatcct ttgctgtcca aggtttattt
     9781 gaaaaataat aactaggggc tacctaaagg tggcccctaa acttggcttc ttgaccagta
     9841 gtcttttacg atatccttta ccctcccgca agccaaaccc tggtggcttt taccctttag
     9901 gtttaaagct ctttaatttt cggcgtaatg gaaacgaatg gtttgcttta gatcggggtg
     9961 caggctttgg gcaaccgggc attccctcga agcagcctca attattcttt tatgcttgtc
    10021 ggaataatca cttgggaagt agaggtcaat aataacctcg acaaccctac ggggattggt
    10081 tcccatgatc ttttgcactt caagccgcgt cccgtcaatg ttaaagccgt gggtttgtgc
    10141 agaaatcccc ataattgtaa ccatgcaggc gccaagcgac gtggccagca ggtcggttgg
    10201 agagaagaac tcccctttcc cctggttgtc aaccggtgca tcggtactaa tctgttcgcc
    10261 tgaacgtgta tgtgtggcaa cggttcttag gttgccctgg taaataatgg ttgaagttgt
    10321 gctcatatta tgtaagattg tgattaccct ttcgtagcta taaacgcata aaagggctca
    10381 aagttgagcc cttttatagt aaaatcatcc aaacaggaag ctggtttaaa ccaagccctg
    10441 gtcgagcatt gcattggcaa ccttgaggaa acctgcaatg ttggcgcctt ttacatagtt
    10501 aacataccca tccttgcctg ttccgttctt tacacatgcg ttgtggatat ccttcataat
    10561 ctggtgtaaa cggacatcaa cctcctcctt gggccagtta agcttcatgc tgttctgggt
    10621 catttccagg ccggaggtgg caacaccgcc ggcattaaca gccttaccgg gggcaaaaag
    10681 aaacttagcc ttagtgaagg cttcaactgc ctcgggtgtg caacccatgt tggaaacctc
    10741 ggcaacgcaa atggagccat tcttgattat taaagctgca tcatcgccat tcagctcatt
    10801 ctgagtagca caaggaaggg caatgtcaac ctttacctcc cagggcttct tacctgccac
    10861 gaacttggag ccagggaact tcttggcgta gggtgcaaca atatcctggt tagagttgcg
    10921 cagctcgagc atgtagttaa tcttttcacc gctaacaccg gcctcatcgt agatatatcc
    10981 atcgggacca gaaatggtaa ccaccttagc tcccaactcg gttgccttta gggcagcccc
    11041 ccaggctacg tttccaaaac ctgaaagcgc aacagtttta ccatttaact tgatattcct
    11101 ggtggcaagc atttcctgaa caaagtaaat tgcaccaaac ccggtagctt cgggacggat
    11161 tagcgaacct ccccagttta gacctttacc agtgagcaca ccagtattct catgggcaag
    11221 ttttttatac attccgtaga ggaatccaat ttcgcgaccg ccaacaccta tgtctccggc
    11281 tggaacatcg gtttcggggc ctatgtactt ccagagctca agcatgaaag actggcaaaa
    11341 acgcataatt tcggcatcgc ttcgaccttt agggttgaag tcgctacctc ccttaccacc
    11401 gcccattggc agcgtggtga gagaattttt aaaaatctgc tcgaatccta aaaacttaag
    11461 aattgaaagg tttacacttg gatgaaagcg caatccaccc ttataggggc caatggcgtt
    11521 gttgaattga accctgtaac cgagatttac atgaatcttc ccctcgtcat ctgtccaggg
    11581 aaccttaaag gtaaaaatac gatcgggctc taccaaacgc tcgataattt tagcggactc
    11641 aaattggggg ttctggttgt aaacctcctc aattgattcc agaacttcac gaactgcttg
    11701 caagtactcc acctcacccg ggtgtttctt ctcaagctct gccattagtt tactaacttc
    11761 cattgcttaa tttttttaag gtactactca aaacaatcta tgaaagggca tcatttatat
    11821 ccccttaaaa gtttttcaaa aatgatgtaa aaaaataata tttcaaaaga aataggggtt
    11881 taaaaatatg atattaagca gatttttacc caaatcgtta acatttaaaa cttacgaatt
    11941 cccatttgat tttaagatga tacccttgtt gttttttgca tcaacctgta tggtaagggg
    12001 tttagaaaaa ttcacccacc ggcagaaggt agtttcaagg aaggctggct gtgcgttgag
    12061 aaactctata tcgtaaatac catcattgag gaatgggtta atggtgaggt aggcaacacc
    12121 aaaagaggta aggttctgga agaaatgtgt tccctggctg ggatcaaccc tgaagctgtc
    12181 gaggccggat tcaacaataa ccctggcttc cgatatttgt gaccatttta ttggaatacc
    12241 cagccaaggg tcgctgctgc cccacctgcc cgggcccacc agcacgtagt ttcgttgcaa
    12301 ctcgatgaat ttattgttga tttcctccac ctcacgggca atctctatag ttttagccgg
    12361 gttaaaccgg tcgggtttta cgtaaacaaa atcctgaata tttttcacca acccatgccc
    12421 aagagcggaa tttgagaaga ttattgcctt ggaaaagtca accgcttccc agttgaattt
    12481 ctccgattga tcggtatcca cgatggggcg aatttggagg aagttgaaca tttttggagt
    12541 gcctcctcgg gggctcatgt tgagggcaaa ctctatttcg atgtggttat tcatctcctt
    12601 ttgcccgatg ctgagcaggt cggtaagaat ctcggcaagg gggaaaattc catgcttcag
    12661 aatgggggca aaggtaacca gcttaaggcc ctcgtcgtag aacccatcgc gtaacatgcc
    12721 gctctgcatg tcgtaggttg agacagtgtg cctgaggtcg ggaattgagt aggcgtcttt
    12781 aatttcgagt ttgaggaggt tcattccatc gtccaccatg ggttcgaagg tatccggatc
    12841 ggtgtttagg gcgtaaaagt atttttgggt ttgcctaata gctgtttcgg gggaggcaag
    12901 ctgaagaatt tttttggggt atttcggtga aaatcttagg gcaactcctc cgtcaactat
    12961 gagcttccct agcccaaaac ctatgcttgc aattccatcg aacggctgct ccgacccaac
    13021 cgggtagaaa ttgatgctgc gggctactcc cgaaagcgtt gggagatacc agctgccgta
    13081 ttcgttgcca caaatttcct ggataacaat gcccatcttt tcctcgtcaa ttacgttctg
    13141 cgtggcgttc atgtatgcct tactggagcg gtaaaatacc gaggcataca ccgctttaat
    13201 ggcctggcaa accatgtaaa gcattttttc ggaatcggag gatcgcggca ccatataggt
    13261 ggagtaaatg ccagcaaagg gttggtagtg ggaatcctcg agtttgctcg aagatcttac
    13321 agccaatggc cgtgtgaaaa ccgatataac ctttcggagg tcaaccttaa gccgttcggg
    13381 tagccgggcg ttcagaaaag cttttaaaac gttttcgtcc gagatatcct gaagagctgt
    13441 atcgtagaga ttgttggact ccatgaactc gtcgaagatt tcggtgctaa ccaccactgt
    13501 acggggtatg cttatgataa tcccttcata cttgttaaaa agcctatgtt tgcgaatgac
    13561 ctggttaata aatgcaagcc cgcgagcctt tccgccaatt gaaccctctc caattcgggt
    13621 aaaaaatttg tactcatcga aggtgttcct gtcgaagttg gcaatcaggc tccttccctt
    13681 gctgagtcgg taggctgaaa tggccttgta aagaaagctg cgaacatcat caatggagtt
    13741 aaagtccttg agggtcatcg gtttaaatag ctcggcaatg gtaaaaatgg cacgtgcgta
    13801 aagccaccgc gatatttcgt tacggctggt gtggtaaacc agcaccctgt cgggaacggt
    13861 taaaatgagc tgctggagct gacttaggtc ggaagcccgg gccacctcgg ctagggtgtc
    13921 ggggtcacga aaaacaaaat ccccaaaacc gaagttatta atcatgtagt tccgcaggtc
    13981 tatgctgaag gttttcgagt acttatgcag gaaaccaact cccatttcat cggcataaac
    14041 cttattgttc ctgtccgatg attgcagcag gaagggaatg ttgggatcgt tctcacgaat
    14101 aaccctgcat aaccttaatc ccgacttggc caggtggtca cgcttgttgt caaccttgta
    14161 gctaatgtcg gaaatgatac ccagcacgtt gtttcggtac cggttgtaaa gtgcaatagc
    14221 atcgttatag ttggtggcca tcaggatttt aggccgacca cgcattcgca gcattcggtg
    14281 gtgctcattt aacgcttcct tcataaagct gcgcgactgc ctgaacacaa ttttatagat
    14341 gtttggcaaa aatgaggagt agtagcgaat ggaatcctcc accagaatta ttgcctgaac
    14401 gccaatttcg agtatgtcgt ggtcggcatt cattttgtcc tctataagct taattatggc
    14461 cagcataata tcggcattgc ccagccagca gaaaacgtaa tcaatggagc ttaaatcctc
    14521 cttctggatt tgaagggaaa cctcgcgcga aaagggggta aggagaacaa tgggtacgtt
    14581 cgggtatttc cccttaaata ttttggccag cctaaaggga tcaatctccc caacgttgag
    14641 catggatatg ataaggtcta ttttctcttc ctggagtacc cgcaaggcct ccctggccgt
    14701 attggcctgt atgaacaccg gcgggtacct aaggttaaga gaaacgtact cgttaaaaat
    14761 ctgctcgtct attcgtccat cctcctccag cataaaggca tcgtagctgc tgcaaataat
    14821 cagcaccttg taaattcgct tttgcatcag cttatcgaac gatgtgtcgt acacatcgag
    14881 ctggtagctg ttcaggtacg attgctctga attttcctcc gacatgtggt attagctttc
    14941 ggtcccgatg cgaatatagc aactttaggt gagattgtta ccctactcat ttatagcctt
    15001 ttgctatgcc ttaatggctg gaactataat gcatgaatag tgccgatgga agggagtcga
    15061 agctaaaaca tgctactata agctggtagg aaaaagggtg ggtaaataac aaaaaaaccc
    15121 ggctctaagt ccgggttttt ttgttgccaa cagaggttac gaaatggcta tctgcttggt
    15181 gggtttaact tttgcttcat ctttcttagg aacgcttatg cttaaaattc catccttgta
    15241 tgaagcttta attttttcgg tatcaaccgt atcgggcaag gtgaaagtcc ttttgaacga
    15301 ggagtaccta aactcccgac gcattacctt ttcgtccttg ttttcctctt ttacttcctt
    15361 ttcgctggaa atttcaagaa cgttgttgtt caggtcaata cggaaatcct ttttgtcaaa
    15421 gccaggagca gcaacctcta tggcgtactc atctttacct tccttaatat ttacggaagg
    15481 cacggtaaca ctttcggtat cgtcaaagaa cctgttccac aggtcgtcgc caaaaaactc
    15541 atcaacgata cttggcagtg tgcttctacg tctcatcatc ggcatcatag ctttaccctc
    15601 catactttaa gttagacatt caatttttaa actctgatac ctaactatca acttgaatgc
    15661 caagacgaaa aagcgccatt ttgaccgaat ggtgtcaact ttgtctgcca tgttgacacc
    15721 aaggcgagaa ggagaaagcg cacaggtgaa aaatgtcagc accagcaacc cctattcaac
    15781 aaaatcatgt ccggttttaa gctattgaca ctttgataat atggcaatct ctgccaagcg
    15841 gcagattttt gggaagcact gtaaacagga gctattcttt ttggccggct ttcataagcc
    15901 gagggtaaag caacgatttc caaagcaagt aacccttttc gttaagatgg agcaaatcgg
    15961 atccaaaaaa atcctcgttg ggagagccat cagggttcag catgggggtg caggtatcta
    16021 caaagtcaat gtattctgaa gagtcggcca tttgtttcat cagctcgtta gccttaagat
    16081 acttactaaa atactcctta cgcgaggggc ttagcttaat agataccagc agtatttgtg
    16141 cccggggaaa atgaatattt accaagcggg ttattgtagt aacatcatct ataaattcac
    16201 caacactttt tgaagtttcg gccaaatcgt tgtcgccttc atagattacc acctgcaagg
    16261 gattgtaggg taaaactatc tggtcgaaat agtaaatcaa atcggtcaaa gtagagccac
    16321 caaatgcccg gttaagaatg ttcgattcgg gaaaatcggc tacagcggtt gtccatcggg
    16381 taaaagtgga gctgcccaca aatagcacac agttggcctt acaaccatgg gtagaatctt
    16441 ccctggcaaa cctctccatg tcgctagtcc acaatgaagc acggctatac tgcgccttaa
    16501 catctactac agctgttgca agcagcaagg aaatgaatag taatcttttc ataccaatca
    16561 aattcaccta aaggttacac taccaagtat caaaaagttg aatatttata gttaaacttt
    16621 cacattcgaa acatgttgca aacggggaga caaatcattt cgttgaaaaa acgtataatt
    16681 ctccaatagg aatccaattt gatgataaga ccgcgggttg aaatgtaaca tttccacatt
    16741 acaaatatac ctttttggaa tttcaacata cgaacgcagg aacaagctat tttcctagta
    16801 tatcaaaaaa tttcagatca tgtaggaatt gctctttact attggcaaac tttttaatac
    16861 cttttggcaa ttttttagta accactaaat ggttgaaaaa gcctcaaaaa ccaaaagtat
    16921 agctacggac tccgctctcg gcgaggagca aggagtggtt gaattcgaca agctgaaaag
    16981 cttggttctt cagaacgaga gcagcatata cgtggttcag agcttccgtg tagtcttttg
    17041 taatcaaagt ttttccgact taacgggcta caccgaacag gaggtaaagg aaatactatt
    17101 cctggacatt gtccatccca aggatagaaa gctaatcaaa atattattcc agaacgattt
    17161 tcaggaaatc aggcagcaca agtcgaacag cttcactctt agaattctca cccgcgacaa
    17221 cgagctgagg tggataaagt cgcatatctc gctaatagcg tgggaaggaa aatcggcgct
    17281 gctatgcacc agctacgaca tcacccagca aaaggaggcc gaggaaaacc ttgccgatct
    17341 ggagcagaat ttcggcatac ttgcaaacac atttggcgat tttatttttg ttctcaacca
    17401 caaccacaac atcattcagg caaatagggc ggtgattgac aggcttggat accaggaaca
    17461 tgagctcttg ctcgaatcat ttgtaaacct tcatgctcca agcgataggt caactgttag
    17521 gagtttactt ttaggagtat ttgctggcac ccactgctcc tactcatcct tcctgctggc
    17581 taaaagcggg tatagaattc cagttgaggt tagaatggtt aaaggaatgt ggaaaagaaa
    17641 ggcttccata tttgccattg cccaggatat caccccccgt attgaggccg aacgcgaggt
    17701 gaaagcatcg gaggaaaagt tctcgaaagc tttcaacacc ggtgccgtta tgatggctat
    17761 atgcaccatg gatggcggaa catacctgga tgttaatgaa gctttcctcc aaaaaacagg
    17821 acttaaacgc gatgaggtga ttggccaatc cctacacgat caaaaaatat actctgatgt
    17881 aaataggctt aaccagctct tggcacagat cagggaaaag ggtaaggtaa acaatgtgga
    17941 agttggcatt agtaaccaaa ggggcgagga gttaaccctg ctgctttcgg ccgaggttat
    18001 cagcattcag gaaaatgact gcatcatgat tgccatgaac gacatcaccg agcgcaagcg
    18061 gatggaggag gaaataacca acagcaggat acagctaaag ggaatactcg ataacctgcc
    18121 attcattgcc tggctcgccg acaacaaagg tcgctacctc ctggtaaaca aatcttttac
    18181 cagtttcttt aacaccgacg agggtagggt tctcgggaaa aaacatggcg atttctgggc
    18241 taaagcccac tcagccaatc tccgaaagca tgaggtggaa gcagctaaag ctaaaaaatc
    18301 attatcgtgg gaggcttttg aaaaaaacga cctgcaggaa gagtggtggg agcactttgt
    18361 tacccccgtt attaacgcgg agaatgaagt gattgccact actggcttag cccgaataat
    18421 taccgatcaa aagctgatta ggctgcaaat tgagcaaaat ctggaacgac aaaaattcct
    18481 aaccgaaatt tcatacctgt tcaacaccgg cgtaaagttc gagaccaaat taaaccaatc
    18541 cctgcaaaag gtttccgggt tgctaaaact caaacgggct tttattgttg aacgtattca
    18601 ttcaaccacg ccttccccaa aggttttcga gtggcacgac agcacagcca acttagacca
    18661 ggatgcgctg attagctcac ttgataagta ttctcctgca attttcgacc atttaaacca
    18721 aaccagccaa ctggtagctg acatttgctc caccgaagaa ccgtcggtga attcgcttag
    18781 caaagccctg tctgcaaaaa atctattcat ctatccgctc atcagcgagg gttgctgtat
    18841 aggttttata ggagttgagt actccagcta caaccctcag actaccggca acaacaggga
    18901 gttcatgctt acgttttcat caatactaac cgcagcactt gaggggcaaa gcaaggagag
    18961 caagcttctg gagagcgaac agcgtttccg gacattctcc gagctgctgc ccgaaatggt
    19021 tttcgaagct aacgacaggc aggtaatagt ttttaccaac aacaaccttc ttgaagcatt
    19081 cggattcagc aagcggaaac tgccggctag cctcaaaatt cacgacctat ttatggcctc
    19141 cgagcatgac aagctaacaa accacctggc caaatcggcc caaaatgggc agcagcagtc
    19201 ggccgagctg cttgctcaac gccgtgataa aaccaccttc cccaccctaa cccatgttcg
    19261 acgagtaaag ctcagcaaaa gcaagtacag gtatattggc gtgatggttg acataaccat
    19321 gcaaaagcaa caggaagccg aactgcttaa ggctaaggag caagctgaag cggcaggccg
    19381 tgccaaggag cggtttcttt cgaccatgag tcatgagata cgaacaccca tgaacgccat
    19441 tattgggatg accaacatcc tgctggagga aaatcctgca accagccagc tggtaaacct
    19501 gaacgccctt aaattttctg ccgaaaacct ccttaccctt ttaaacgaca tactggattt
    19561 cagcaagata gaggcagaac gggtagatct ccttataaat gaaaccgaca ttcactcgct
    19621 tcacgccgga atattgagct cttatgcgca gctggcggct caaaagggca ttaagctaac
    19681 cggtagcatc agccccttaa taccgcataa gcttttagcc gatagggtac gccttaacca
    19741 aatactcacc aacctggttg gtaacgccat taagttcacc tccaatggag gcgttgactt
    19801 aagtatttcg cttcaacggc agcttaaaaa atcggtttgg gttaagttta cagtaaccga
    19861 tacgggcatt ggaataccta aggataagca gaagaaaatt tttaccgagt tcacccaggt
    19921 tcacgaccag ggaggcaagt acggtggcac agggctaggc cttgccatat caaaccgttt
    19981 ggtaaatctt atgggcgggc aaatcaggct aaaaagcgta ttcggtaagg gttctacttt
    20041 ctacttcagc ataaaacttg atttggaacc ctaccatgaa acgccagagc ccgctgaagc
    20101 acccgaaatc aagcttgacc ccaccagaag ctacctggtt cttgtggtgg aggataacga
    20161 gttgaacacc cttattgttc agcgatttct taccggatgg ggaattaaca accaccacgc
    20221 tgcgaatgga gtggttgcgc tcgaaatggc taaaaataac cgctacgacc tggtgcttat
    20281 ggacctggaa atgcccgaaa tgaacggcta tgaggctaca aaagccttgc gaaggttaaa
    20341 aggaaaaacc aacaattcgg ttcccataat cgccctgagc gcatcggcaa tgctcgatgt
    20401 gaaacgaaaa attttcagca tcggcatgaa cgattttatt ctaaaaccct tcaaacccgc
    20461 cgatttgaaa aaaaaggttc tattttatct ttctaaagtg taggatttgt atgcgaaggt
    20521 ttattgtttt ctttgcagct gtttctagcc tatcattttt tcccctcgaa aaaaatgcgg
    20581 cacaggttac catagagctt agggtaaaag aatcaatagg ctctacttcc aaactgtcgg
    20641 tttatactgg taaagaatat gttaccgttg attcatgccg ccccaccatt gaggggattt
    20701 accgtttcaa cctgccagaa acggctcctc ttgggctcta caaactaacc gtaggcaaat
    20761 cggggtcatt tgacctaatc gtttcgggcg aaaagcatgt agcgctccaa acggttgcct
    20821 ttgccgttga ggatagccta agggttattg actcggatga aaacaaagtt ttttttgatt
    20881 acatcaaact caagaaaaca tccgaacaac agcattggct tattaaatcg ctgctacaat
    20941 accacacttc cggtgatgat gtttatgaaa agttggttga cgaacagcgt cgcctaacgg
    21001 attcttttga tagcagggcg tcggagctgg cgcaagcgta cccatctcta ttcgtttcaa
    21061 gctacatcaa cctggatact aagctgcaag ctacaggaac aaaaagtgat tgcaacagca
    21121 gaaggatgaa agccgatata tggtggaatg gggttaacct gctggataac aggctgctga
    21181 acacacctat acttattccc cggctctgga gctacattga aagcctgctt tgcgaggatg
    21241 aactcggtat ggaggagcag gattccattt ttgtgcacta catccaaaag ttattcgccc
    21301 ttcccctttc ggaggaaatc cgtaacaagt ttgccgaaac actagccgac ggatttgccg
    21361 aaaccgacta cttcgaagta atagactacc tagcgcacgc tggcggtaaa gcagccgcaa
    21421 cggtttttga taatcccgat tttaaaacaa ggctggaact tgagcgtccc ctgatggtag
    21481 ggagtaaagc tcacgatttt acagctaagc tcatggaagg tgaaaaattt aagctgtcta
    21541 ggagtgtatc ccggtatgaa cttattgttt tctggtcggt ttggtgtccc cactgcgttg
    21601 aaaccctgcc caaaatcaga agtatctatg aagagttcag gcataaagga tttgacgtgg
    21661 tggctgttaa cattgatgag gaacaggagc catggaagga gtttacaagc aacaacagtt
    21721 tgcaatggca caacacccag gtgcctttta gctccaacag ccccataccg gagtactaca
    21781 acgttgacgg aacgccaaaa atgtttctgg ttgacaactc gctccgcata ctgtcgcgac
    21841 ccacgtcggc tgcccagcta catgccacgt tacgaaaact ttacggtgaa taacaatagc
    21901 aggcaatatc ctgtaaacag cagtagcaac cctgctatct gtttataaaa ccaatatatt
    21961 tggctaaatc tccccgaaag gcatcaaccc gaaccggcta ctccaggtag gtgtaaccat
    22021 aaaggcctga gcggtagttc gatagaaact ccttaccctc cttggcagaa atgataccgg
    22081 ctttcaccga cgtagtaacc caggtttcga gcgttcgcac cattttcttg gggttatatt
    22141 gaacgtactc cagcacctcg gccaccgtct cgccatcaat aatttgatca atctcgtagc
    22201 ccttatcgtt aaccgatacg tggacagcat tggtatcgcc aaaaaggttg tgcagatcac
    22261 ccagaatttc ctggtatgcg ccaaccaaaa atgcacctat atagtatgac tcattaggtt
    22321 ttacggtatg aaccggcagg tagtaggaaa tattacgggt agaaataaag ttgtcaatct
    22381 taccgtcgga atcgcatgta acatcctgaa gggtagcagc ccgatcgggc ttttcgttaa
    22441 gtcgctgaat gggcataatc gggaatattt ggtcaatagc ccaggtgtcg ggaagggact
    22501 ggaaaaggga aaagttgcaa aagtatttat cggccaacaa cttgggaagc gaccggaact
    22561 cgtcgggaac gtgcttaatg tcggcagtca tctggttgat atcccgggca atagaccaga
    22621 atagcctctc cacctgtgcc cgggttttta ggtcgagcag ccccaggcta aaaaggtcga
    22681 gggcctcttc ccttatttgc tgggcatcgt gccagctctc cagcatttta gactggttta
    22741 gcttgtccca gatttcgtat agctcgctaa ccagctcgtg cgggttttcg ccaatatcct
    22801 cgttctcctc ccagcttggt agcgaggcgg tttcaagaac atcgaagatg agaaccgagt
    22861 gatgggcggt tagcgaccgt cccgattcgg ttataatgtt gggatgggga atgttattct
    22921 tatcgctgac atcaactatg gtggatacgg aatcgttcac gtactcctga aggctgtagt
    22981 ttatgctgct ttcgctgttc gatgagcggg ttccatcgta gtcaacccca aggcccccac
    23041 caatatccac aaattcgatg ttgaaaccct gaagatacag ctgcacataa aactgggagg
    23101 cttcccgaag ggcaagttta atgcgacgaa tcttggtaac ctggctacca atgtggaaat
    23161 gaactagctt gagggaatct ttcataccgt taccttccag aaactccaac gcctgaatca
    23221 gctcactgga ggtaagccca aacttgctca catcgccgcc ggagtcctcc catttaccgc
    23281 tccctacgct tgccagctta atccgaattc ccaggttggg cttaatcttt aaacgttgcg
    23341 aaaccctagc caccagctta agctcgtttt gcttttcaat cacgatgaag atctttttcc
    23401 ccatcttctg ggccagcagg gccagctcaa tgtagtcctc gtccttatag ccgttgcaaa
    23461 ttataagcga ctcattgtcg ggattgctgg caataacggc atgaagctcg ggttttgaac
    23521 cagcctcgag cccaatgtta aacttcttgc catggttgat tatctcctca accaccggcc
    23581 gcatctggtt cacctttatg gggtagatga tgaagttttg ccctttgtaa ccatattcct
    23641 gggcagccac ctcaaacgag cgggacatat cctcaattcg gttgtccaga atatcggtga
    23701 accggatgag cattggcgca gaaacatccc gaacctgcag ctcatcaacc aactcgcgca
    23761 gatcaatctc taccccatta ttcttaggtg ttaccactac attgccgtta ctgttgattg
    23821 aaaagtaatt caccccccag ccgtttatgt tgtaaagctc ggctgaatcc tcaatgcgcc
    23881 attttctcat tccgggttat aatgttatgt ggttatgaat caacggatta ataaatgatt
    23941 ttatggggct aaattagcct taaaatctta ctatacaaag ggaaagctga aatttattta
    24001 gtgcaacaca agcatattac ccctaccaac atgaattgcg gggtgaaaaa tgaataacca
    24061 ctagtagcac aaaacggcta ctagcaaaaa atgaacggga tgtacaagtg cattggccag
    24121 cttgtggttg ttcaatgaaa taaggaaagc acagagggat ccatttaggc catagctata
    24181 cgcctgtgta tgccctggct tgtggaaagt agtatagtca aaatgtaacc cggcacgaaa
    24241 gtccaagtgc tgaatgggta tcagcggttg cggtaagggc agggttaaca attgcaggct
    24301 caaccctaaa gctcaggttg tcgtcaacat tccaatcgtg aaggaatcca tccacgttgg
    24361 cgtccagaat gttgatgatg tagatggcgg taaggccaag aatgctgaga tccctattcc
    24421 ggcggtagct atccatatag tatcgcagct catcagcagt tctagcacca ccaaattcat
    24481 ccaccgtggt gtcatcacca tcaatattca gctttaaagc ggttttaaag cggctgtagt
    24541 acctgtcatt ccagctgacg aggtagtaaa gtgtcgccaa gccgccgtaa actacaggaa
    24601 ttttccagta cttttggttg taggcttgcc ccattcccgg taccagagtg gaaagaatgg
    24661 tagcagctgc aggtgagtgc ttgctattgt tgtaaaccag cacggcatcc atggctcctg
    24721 ccacatagaa tgccccggca gcggcaatat agaggttgcg ggtttgcctt tgcttcacca
    24781 ttcgctcttt atataattca cggttggggt ctgtggaggt caaactatta taggccgaaa
    24841 gcctgcttag gtaggtttgg ttggccttta ccccaaggta aatcatgctg cccatgcctc
    24901 catatagaac cggtagcttc cagtactggc ggttagcaat ttgcccgtaa ccgggcagca
    24961 gctgtgagcc aagccagatg cgggtggtaa aatctttagc agggatcgcc ggcttaatat
    25021 tttcctgagc ccctgctact ttgccaatac atgtaagggc taaaaacaga atagccgacc
    25081 tagtcaagcg agcatgtcgg ccatgaatca tggaaatagc ctttgttctc aacgttgcaa
    25141 tattatttcc gtcttgctgc tttctgcctt tactcttcaa ttttggtaaa cttatcaata
    25201 ataacgctaa gctcatcagg gttgttgtag gaaatgacaa ttttgccatc gccctttccg
    25261 cctggcttta catctgcacg tagtccgagc ctgttggtga ggtgttccct caaagccagc
    25321 tcaggcccgg caaggtcggg catgacttta gacctggact gcttgatttg gggttcagaa
    25381 acggtatttc taaccagctc ctcggtttga cgaaccgaca gatcgtcaga aataaccttc
    25441 ttgtaaagtt tgagctgaag cgaggcatcg tcaatgctta tgagagcacg ggcatggccc
    25501 atgctgatta ttgagttgat gatacccagc tggatttcgg caggaagctt gagcagcctg
    25561 aggtagttgg atatggttgc gcgtttttta ccaacccttt ccgaaagggc atcctgggtt
    25621 agactgcatt catcaatgag gcgctggtag ctaatggcca cctcaatggc gttgaggtct
    25681 tcgcgttgaa tgttctcaac gagcgacatt tcgagcattc cctgatcatc agcagttctg
    25741 ataaaggcag gaagggtttt caaaccggca agtttggcag ctctaaagcg tcgttctccc
    25801 gaaataagct ggtaccgtcc atccaacaag cgaactgtaa taggttggat tattccgagt
    25861 ttggaaatgg actcggccag ctcgctcagg gattcatcat caaaattggt tctcggttga
    25921 aaagggtttg ggtcaatcag ctcaatgtca acctcctgga cgagttcgga gatagcatgc
    25981 cttggtgtct gtacatcgct gtttcgggtc gattcgaaag ttgaatcctc aataagcgct
    26041 cccaatcccc ttcccaatgc cattttttta gtcatagttt tatctcccag cctatgcggt
    26101 aacggttaat cctgggcaat cttttgctca ttctcaatct gagtctggtt gtttttctga
    26161 agcagctccc tggccaggtt gatgtagttt gccgagcctg tagaggccgc atcgtagagt
    26221 attacaggtt taccataact gggggcttcg ctaagcttga tgttccgctg gataatggta
    26281 tcaaaaacca tttgctggaa gtgcttgcga acctcctcaa ctacctgatt cgataatctt
    26341 aacctcgggt cgtacatggt aagcaggaat ccctcgattt gtagctcagg gttcaggcgg
    26401 gattgaatga tcttaatggt gttcagcagt ttgcctagac cctcaagggc aaaatactcg
    26461 cactgcacag gaatcatcac cgaatcagcg gcagtaaggg cattaacggt gattagacct
    26521 agtgatggtg aacaatctat aaatataaaa tcatagctat cctttacctt ggcaaccacc
    26581 tcttttaata ttttttcacg gttcggtagg ttaagcatct caatttcagc ccctaccagg
    26641 tcaatatgag atggtatcag gtccaaaccc ttgatttcgc tgttcaagat gatatcgttg
    26701 gggtttacct catcaatcag gcattcgtaa atgctggttt tgatgtttct aatatcaaac
    26761 ccggttcctg aagtggcatt ggcctgtggg tcggcgtcga taagcagaac ctttttttcg
    26821 agcactgcca gacttgccga caaattgata gcgctggtgg ttttacccac cccacccttc
    26881 tggttggcta ttgcaataac ttttcccatt acctaatctt taacattaaa acaagcgtca
    26941 aaaatacaaa acctcccgca accatcctgc ccccaccgct ggggaatatg aatagtattc
    27001 aacaggatat aaacatgttt tcaacaaccg atttaatcat acttaccggc aaagatagaa
    27061 atttttcaaa aaaaaggttg accctgcctt aagccataca attaaacttc tatgtgctat
    27121 ctttgcagct ggaattcgat gctaaaatct atctgattgg taaacagctg gtttgttatt
    27181 gtaaaccccc gggcaggtgg gggtaagggt gaggttgact ggccgctcat caagggtttg
    27241 ttgactaagg aggggatagc ttttggattt gctcttaccg aacataagca ccatgctgtg
    27301 gagcttacgg tgaaagccat caatagcggc tacaggcagc tgattgccgt tgggggtgat
    27361 ggaacgctca acgaggtggt aaacggcatt ttcatccagc agtcggttcc acctgccgag
    27421 attctggttg gagtaattgg cgtaggaacc ggcaatgact ggtttcgaat gtacgaaatt
    27481 ccatacaggt acgccgatag cgtaagagca ataaaaaatc gtaaggtttt ctaccaggac
    27541 gtaggtgtgg tggagtacta tgagtctagc gtaaagcata agcgatactt tgttaacacc
    27601 gccggaatag gctttgatgc tgaagtggcg ctccgaacca acaggttaaa ggatttggga
    27661 agacatggtt ttttgctcta cctcatcagc atccttcgag ctctttttag ctacaaaaca
    27721 acccggataa acgtaaatgt ggatggccgc catttacagg ataaaatttt cagcattacc
    27781 ctggggattt gccgttacaa cggcgccggt atgctacagg ttccctttgc catctccgat
    27841 gatggaatgc ttgacgttac cgttatccgc agaatcagca agctggatgc tctaaccagc
    27901 attccccggc tctacaacgg gaaaatctta aaacactcca gggtaaccgg actgcgtggt
    27961 caaatagttg aaattatgag cattccctca actaatcttg aggccgatgg cgaatcgcta
    28021 ggcgaaaccc ctctgcgctt caccgttctt acccgtgcaa taggcgtggt ggttgacgaa
    28081 aactttgatg ctaaggataa ccgccctgct gttattcagg agaaacaaac cctctcggca
    28141 gtctaatcat cctgcttccc gcttattaga gtttccattc acccgcacat ctaccctggg
    28201 cattactttt tcaacattta tatagcagcc aatactagac aaccccctag attcgtttct
    28261 taccagcagg catcataccg aaaaaacatg ccggggtaac cattaagatc aaaactcaaa
    28321 aaaatcatca ttaacatcta aaatgaacgg tatgttggaa cctcctcgta acaggtttta
    28381 ctttggttac gtctgtgctt atgaagcatg gaggtttcat attaatttga tttgtttact
    28441 tttacgagga aaaat
//
LOCUS       DAOPHX010000017        26920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_1782_length_26920_cov_6.293840, whole genome shotgun sequence.
ACCESSION   DAOPHX010000017 DAOPHX010000000
VERSION     DAOPHX010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 26920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26920
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1782_length_26920_cov_6.293840"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..614)
                     /locus_tag="PK502_02445"
     CDS             complement(123..614)
                     /locus_tag="PK502_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99709.1"
                     /translation="MEKGAKGNITGNQLEVAVKTVLTGKGFELINYRVWDKNKEKYGE
                     ELLLENVPFTTIYNHKGNTEFLLISKRFDLQIRIECKWQQVKGSVDEKLPYLYLNTIE
                     AMPENTIMILIDGAGWKTGAVKWLKDAVQQKKYATAETQNKEIIVFTMTDFFTWANNT
                     FNK"
     gene            complement(615..1604)
                     /locus_tag="PK502_02450"
     CDS             complement(615..1604)
                     /locus_tag="PK502_02450"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HPG99710.1"
                     /translation="MTQAIKYKIKDDNVSTSEDLFNHFNVGAKPFLKWAGGKGQLLDK
                     FQEFYPKLLKQKEIKTFYEPFLGSGAVFFDIAQHYDIEDAYLYDINEELILTYKVIQK
                     DVTKLLDFLYQYQKKYIKLDKKQRQVFFYDQRANFNLQRSNINFDKYSEQWFPRAAQL
                     IFLNRTCYNGLYRVNSKGEFNSPAGDYDNPTICDEQNLLAVHQVLKIAVIKKADFKEI
                     INDIKAKSFVYFDPPYRPISKTASFKAYSKQDFAENEQIQLAKLFRQLDNKGTHLMLS
                     NSDPKNYDPSDNFFDDIYRGYNITRIPAKRMINSDPTKRGAVNELVVTNYQIA"
     gene            complement(1898..2737)
                     /gene="prmA"
                     /locus_tag="PK502_02455"
     CDS             complement(1898..2737)
                     /gene="prmA"
                     /locus_tag="PK502_02455"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPG99711.1"
                     /translation="MAAFTELRIKTSPFSEETNEIIIAQLAELGFDSFMESESELLAY
                     SSIHSVSAAINTLHSIAIPAGVSIDYSAGETGERDWNEEWETGFEPVTVDGRCHIRAS
                     HHPVNPAAEYNITINPRMAFGTGHNPTTYMMLEALMDMNVEDCRVLDMGCGTGVLAIM
                     AELRKARSVTAIDTDEWAYRNTLENIRINGCSRVEVLLGDAQLLANREFDLILANITL
                     NVLLANLPAYAQSLVCKGHMLLSGILTTDVDRLLQYAKSIGLEPVATRTREGWAMVQL
                     HRS"
     gene            complement(2737..3492)
                     /gene="tpiA"
                     /locus_tag="PK502_02460"
     CDS             complement(2737..3492)
                     /gene="tpiA"
                     /locus_tag="PK502_02460"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPG99712.1"
                     /translation="MRKKIVAGNWKMNTSLAEGFSLAKEVIAGSSRVDQGVELVVIPP
                     FTHLSEIVKLARGSRVRVGAQNCAQWPKGAYTGEVSASMLQSAGVEYVILGHSERREY
                     FNETNRSLFEKLQLALQENLTPIFCCGEKLGEREAGQHFAVVEKQVAESLFGLETRQV
                     QQVVIAYEPVWAIGTGKTATADQAQEMHAFIRGLLAGKYGSTLADSISILYGGSCKPS
                     NTAELFAKPDVDGGLIGGASLVAADFIAIAGSF"
     gene            complement(3556..4638)
                     /locus_tag="PK502_02465"
     CDS             complement(3556..4638)
                     /locus_tag="PK502_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG99713.1"
                     /translation="MIRFVIKRILYFILVVFGVVTLIFALFHMVPGDPARMVMGQRTD
                     SASLAAARADMGLDRPVAVQYLKYLNDLSPLSVHNPNNPKSYIYLDTLLYSGIVNLVK
                     LSDNRQLVLKLPYLRRSYQSRKPVTSIIAETLPNTFILALVAIIFATIVGIAMGIIAA
                     IRKDTAVDRMLLITSSFGMSLPSFFAAILVGWVFAYLLGSYTGLNLTGNLWEVDDLGE
                     GVHLQLKNLILPAFTLGIRPLAEITQLMRSAMLDTLNQDYIRTARAKGLKFRHIIMRH
                     ALKNSLNPVLTAISGWFASLMAGVIFVEYIFGWKGLGFVMVTALNSYDVPLVIGCVIS
                     ISLIFGVVNLIMDIIYSLLDPRVRMG"
     gene            complement(4702..5511)
                     /gene="mazG"
                     /locus_tag="PK502_02470"
     CDS             complement(4702..5511)
                     /gene="mazG"
                     /locus_tag="PK502_02470"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPG99714.1"
                     /translation="MDKKTDNITRNERAREAFDRLLTIMDELREKCPWDRSQTFETLR
                     PLTIEETYELSEEILDKNFQAIKKELGDILLHIVFYSKMAAEENQFDMAEVINSLCDK
                     LIYRHPHIYGNVEVKDTNQVVENWEQLKITEKDGNKTVLSGVPASLPSLIKANRIQEK
                     VRAVGFDWEERGQVWDKVEEELEELKVEVERQNRNRIEGEFGDLLFSVVNAARLYNID
                     PETALERTNRKFMKRFGYLESKTIKQGQSLKDMTLDQMNAIWEESKQFDTE"
     gene            complement(5534..6379)
                     /locus_tag="PK502_02475"
     CDS             complement(5534..6379)
                     /locus_tag="PK502_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HPG99715.1"
                     /translation="MLIFNIPDYSITFVMLLANPMDNLRIVTLTTDWNQNDYYVGILC
                     GRLLSIEPTLRIVELSHRVPPFDYIQAAFIMRGCFENFPPGTIHICMVNSDSTTSKQL
                     LLFELKGHFMIVPDNGIVGLITEVAPDRVYSIPFEEHSSFGSLSAVASAMNMLLFNSN
                     LADIALIAGTYKQNINIRAASDESSITGSVIYVDSYQNAITNITREQFEQVRKGRKFS
                     IYVQSFSYKIEEIVENYADVEDGDMLALFNSLNLLEIAIRNGFLTELLALSVGSSIMV
                     KFTNR"
     gene            6800..7807
                     /locus_tag="PK502_02480"
     CDS             6800..7807
                     /locus_tag="PK502_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPG99716.1"
                     /translation="MVGERIITVEGVDPLAIYGVNNTRFEMISRHYPKLKLVARGNQI
                     KVTGDEELIDDLESKINRMVEFYSRYSRLTEANILDFLNDNDSSIVKAEEDALDVLVY
                     GTNGRTVKARTINQRKLVELYQTNDLLFAIGPAGSGKTYTAIALAVRALRNKEVKRIV
                     LTRPAVEAGERLGFLPGDMKDKLDPYLQPLYDALHDMIPQKKLMEYMDDGTVQIAPLA
                     YMRGRTLDNAFVILDEAQNTTLNQLKMFLTRMGKNAKFIVTGDITQIDLPNRGDSGLV
                     KAIGILENIGSIAMVYFDARDIVRHKLVKHIVNAFERYTSGNGSSERTENNDECEDNS
                     Q"
     gene            7979..8926
                     /locus_tag="PK502_02485"
     CDS             7979..8926
                     /locus_tag="PK502_02485"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPG99717.1"
                     /translation="MEAIVRTDFKFPGQTSVYHGKVRDVYNIANKHLIMVATDRISAF
                     DVILPKGIPYKGQVLNQIAAKFLDATADIVPNWKIATPDPMVTVGRLCEPFKVEMVVR
                     GYLTGHAWREYKSGNRLLCGVPLPEGMREHQKLPAPIITPTTKAIEGHDEDISREEII
                     ASGLVEEHDYAQLERYTLALFERGTRMAEKMGLILVDTKYEFGRADGRIYLIDEIHTP
                     DSSRYFYADGYTERFNRGEEQRQLSKEFVREWLMANGFQGQEGQKVPEMTPEFVNQVS
                     ERYIELYEQITGEKFQRAEGSDVMKRIERNILNFIKTLV"
     gene            complement(9013..11901)
                     /locus_tag="PK502_02490"
     CDS             complement(9013..11901)
                     /locus_tag="PK502_02490"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPG99718.1"
                     /translation="MPATLKPFLALTLQHHRQLGTTIGAYLATPTESHGIFIISENAA
                     VAQPSDLEDIVSPIQKDMLQQMQQISDKALQKYFDKQTKSVAEFLDSLTRSEQLNSHV
                     HSYVERRIAKVVALAAHSDTPIFLRKKEEKNLYEENRLNVELEPTLPLFRFELTPDSI
                     MYSIMAMNNGRTVNLTTTNGEVVTNNPCIIRIDRTIYQFEAIDGKKLTPFLSKPYITV
                     PKSAEKKYMQTFVLNAIRHQQVEAVGFNIVDEQHDGRAILTLEERLAGGWCLTLKFSY
                     GDRGYLCNTPYNTEVRLVEEGNGYTFRRFNRNRIWESRVICLLSEKFNLQRVSEAEFL
                     PAAVENAKENLYPLVEWLNQHVNQLKTEGFAVAQLVRGMNFYLNGVVVAISTDSTTDW
                     FDLYGKIKLDGFEIPFVYLKRNILKNIREFLLPNGQVFIIPEVWFSRYKPLMLIGKEE
                     NEGIRLPKSLFGLLADTNEVNSPAASELRHRLSNLKKEGIQLPQRLNARLRSYQLVGY
                     AWLKLLNQNGINGCLADDMGLGKTIQALTLLQSINEEQNYATGMGKTSLVVVPTSLVA
                     NWISEAGRFTPELKVAAYMGQQRVKSASWLLNHHVVVTTYGLIRNDIDMFREVTFRYV
                     VLDESQTVKNPLTKGYRSVMQLRAINYLSLSGTPVENSLLDLWSQLNFLNRGMLGGLR
                     AFRNEFQTPIERQGDHEKEKQLKQLIEPLILRRTKEEVAKDLPEISEQVVYCRMSDSQ
                     KEIYEKEKSVVRNFILETLDSPAPNQSTVSILASLTRLRQIANHPAMLPEYRLADSGK
                     FDEVAMGIESATAGHHKILVFSSFVRHLEQVEVFLQDRKIGYEKLTGSSANRSEIVER
                     FQTDPDKKVFLISLKAGGVGLNITAADYVFILDPWWNPAAEMQAASRSHRIGQDKKVF
                     VYRFITSETVEEKILSMQERKENLAQLFVNSSSSLGQISREAILELLE"
     gene            12537..14423
                     /locus_tag="PK502_02495"
     CDS             12537..14423
                     /locus_tag="PK502_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPG99719.1"
                     /translation="MRSYITALLLCLSPMLLAGQVVEGDIPSVQRSTDKVKIDGKNYY
                     IHIVKSGETLYSISKAYEVSQSDIAVNNPDIYNGLKIGQALKIPVRASESKGTDDYIY
                     HIVAKGETLYSISRRYNIPVDVLTSINPEVKDGLLVSQVILIPRNLMIPEAKKPAADT
                     VQFIMHEVQKGEGLLAVARRYNIDAKTIETYNQEVLKDGLKLGEYLRIPAVKQHPADS
                     LMVTSKPEPVAFFSTCKNNFRYNGQPFDISLLLPFSQGSAVDSDELIDEAEEVKMDHG
                     TTNTLESSRITSQETLASLNFYEGFLLAVDSMKKAGVSMNINVFDTKHQPEEVTSILN
                     KGKLDKSNIIIGPLFVNEIKPVVAYAERKSIPMVSPLYSGNEVISEYPGLISVNQGYG
                     EQLEVFIKNFKFSDTCHYVVIYDMEKILTGPIQNFSSALNQRIRESGVRVDSVVHQTA
                     AMKSEEVQAKLHSYMKADGCNVVIIPSQDEPFVSDLLGQLYAVKSLNGLKMIVYGPAR
                     WQKMKNVPPDYLYKLNARVFTPFYVDYSRDEVKRFVASFRDRYRAEPNQFSFLGYDVG
                     IYFISALKTYGPDFTKCLKDFQKDMLQSTFTFDVVSNESRLSNTHQFIIGYTDDYDLV
                     RIIP"
     gene            14747..16366
                     /gene="pckA"
                     /locus_tag="PK502_02500"
     CDS             14747..16366
                     /gene="pckA"
                     /locus_tag="PK502_02500"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HPG99720.1"
                     /translation="MANLDLSAYGVNQPAEVFYNPSYELLYQHETDPSLQGYERGIVT
                     KLGAVNVDTGVFTGRSPKDKYVVKEPNTEKNVWWAQPGKKSSDNKPITQEVWNHLKDI
                     SAKQLSGKKLYVVDAYSGANEDTRLCVRFVMEVAWQAHFVKNMFIRPTDEELKNFKPD
                     FIVLVASKTSNPKWKEQNLNSEVFVAFHLTERMALIGGTWYGGELKKGIFSVMNYFLP
                     LKGIASMHCSANVDQKNGNVAIFFGLSGTGKTTLSADPQRALIGDDEHGWDDNGIFNL
                     EGGCYAKCIDLDPEKEPDIYAAIRRDALLENLDVDSEGNIDFHSKVKTENTRVSYPIY
                     HIQNIVKPVSKAGHAKHVIFLTADAFGVLPPVSRLSPEQMQYHFLSGFTAKLAGTERG
                     VVEPQPTFSSCFGQAFLMLHPTVYARELVKKMQLHGSKAYLVNTGWIGGPYGVGKRID
                     LPSTRAIINAILNGSIEESEFELLPVFNLQVPLRVNGVNPHLLNPQKSWANPGDWDKA
                     ARNLAGMFIKNFENFTDNEWGKSLVKAGPHI"
     gene            16770..17426
                     /gene="plsY"
                     /locus_tag="PK502_02505"
     CDS             16770..17426
                     /gene="plsY"
                     /locus_tag="PK502_02505"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPG99721.1"
                     /translation="MEILSKIAIVFLAYLMGSIPTSVWVGKIFYGVDVREHGSGNAGA
                     TNTIRVLGLLPGIVVFIVDVLKGYLAVRLLYLTDFYIPKTGMFINFQLILGIAAMIGH
                     IFPIFAQFRGGKGVAVLSGVVFALHPYATLIVFGIWIVSVIITGYVSLSSMIAGFSFP
                     LILIFVYRTTNPSLILFAFILAILMLFTHQKNIERLFKGEESKFHLKKKPHNEPDKPK
                     "
     gene            17488..18204
                     /locus_tag="PK502_02510"
     CDS             17488..18204
                     /locus_tag="PK502_02510"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HPG99722.1"
                     /translation="MQIFYAPNITGSSYTLPEDEARHCLRVLRMRVGDEVELVDGKGN
                     LFTGRIANPDLKECTLEIVRKASNFGERHFYLHIAIAPTKNADRFEWFAEKVTEIGVD
                     EITPLLCERSERKSVNSERIERIVISAMKQSEKAYLPTLNTMTSLEKLVASTPVDFVK
                     LIAHCNEKGIPHLKKMVKPGDKVLILIGPEGDFSPKEVDFALINGFRAISLGNSRLRT
                     ETAGIVACHAVNMLNTEDFS"
     gene            18235..19041
                     /locus_tag="PK502_02515"
     CDS             18235..19041
                     /locus_tag="PK502_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG99723.1"
                     /translation="MRRLLVGGYRFSWFHIAAALLGGVVLLFIVAPILGMIISTPAKS
                     LIETAAEQEVVRSIWLSVWVAMAATLVFALFCIPLAYLLARFDFPFKRLVLGIIDLPI
                     VIPHSAAGIAVLGLIARDGLLGRLTSPLGIELVGSPAGIAIAMAFVSVPFLINAAREG
                     FALVPERLEKAALSLGASPITIFFTVALPLAWRSVVSGLVMMFARGMSEFGAVVIVAY
                     HPMVAPVLIFERFGAFGLKYAKPVAVLFIGVSLTVFIIMRLLSRNRSNHA"
     gene            19034..20098
                     /locus_tag="PK502_02520"
     CDS             19034..20098
                     /locus_tag="PK502_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG99724.1"
                     /translation="MLRVEGVCKKFQDFSLNQISFTVDQGDYFMLLGPSGAGKSLILE
                     TIAGLHKPDSGKIFFQGVDITHIGVRERNFGIVYQDYAVFPHLTVYGNIAYPLRRKGI
                     RGKELHKQVTRFAERVSVAHLLARHPQTLSGGEVQRTALARTLAMEPRILLLDEPLSS
                     VDVKLRSELRSLLRSINSSGQTVIHVTHDYEETLALASHLAVVNGGCIEQVGSVQEIF
                     QNPRSEFAAMFAGVHNFYPASIVEFSEHGLTMVRLNDTIAICCYTEGCNGEGFVCFSE
                     ESVILSTEKPHQSALNVFRGRVVEIFPQRFGLMAVVDAGFRVLSKVTWESASRFGLTP
                     GVEVWVSFKASSVRFIPRAS"
     gene            complement(20100..20951)
                     /locus_tag="PK502_02525"
     CDS             complement(20100..20951)
                     /locus_tag="PK502_02525"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPG99725.1"
                     /translation="MRQSGVRCIIFLLLPLVVASCLKPGSYNIRERYVVKAKTLIVRS
                     SPSTASAPVVPLQQGDTINAIVSDTEWVTVKVGTKKGYVAASYLNRIEPTPTPNLLSW
                     VEKTASWESWVFWLIAIPAIVIWIFSGKLIVQYKNRLAQKHDFQVKGLVLMPVVFFIN
                     ALLLAILYINWKDLLLNSIEHGIRAIPNNPDIIDWVVWCQALSLLVSILVDLLGTVIK
                     SGILWGVVLTFVDLLWSVFIFAATFFLTLSLYYYAIVFLLIFFGIQYVSMVYQNSRKI
                     SSYSRPR"
     gene            complement(21188..23806)
                     /gene="alaS"
                     /locus_tag="PK502_02530"
     CDS             complement(21188..23806)
                     /gene="alaS"
                     /locus_tag="PK502_02530"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPG99726.1"
                     /translation="MTSAEIRRAFLNFFEGKSHTIVASAPMVVKNDPTLMFTNAGMNQ
                     FKDIFLGNTPAKFKRVANSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     NEAIDWAWEFLVDVLKIDSTRLYATIFEGSPDEGVEKDYEALNCWAKHLPSNRIIEGN
                     KKDNFWEMGETGPCGPCTEIHIDLRGEDERKANPGEKMVNTGHPLVIEIWNLVFIQFN
                     RKANGQLEPLPAKHVDTGMGFERLSMVVQGKKSNYDTDVFKPLIDRISNISGLKYGAD
                     AKSDVAMRVIADHLRAICFAIADGQLPSNVKAGYVIRRILRRAVRYGYTFLNFREPFM
                     AGIIPVLTEQMGDQYPELRKQQSLITSVITSEETAFLRTLETGMRMLDQLVERARKEH
                     KMEISGKDAFVLYDTYGFPLDLTELILRENGLSVDHSGFDGEMALQKERSRADAAVEE
                     MDWIVVNDDENAEFVGYDTLTCNVHIARMRTVRVKSNEQYQLVLDKTPFYAESGGQVG
                     DSGTITGEGEAIRVLNTIKENNLTIHIVDKLPANPNGTFVASVDVNRRASTAANHSAT
                     HLLHNALRKILGLHVEQKGSLVHPDYLRFDFSHFQKMSEDEIRQVERLVNSVIRENVH
                     LDEKRNMPIDEARSMGAMALFGEKYGDRVRVIHFGESVELCGGTHVEATGRIGFFKIL
                     SETAIAAGVRRIEAITGIKAEEFVYSQEDTIKEIKSLLGNPSNVSQTVARMVADNEML
                     RKEISSLQAEKLAEVKHQLKQSIEQRNGINVICQKVGLNDSTTIKDLAFQLRNEVPNL
                     FLAIGATASEKAILTVMISDNLVQSLQLNATSIIREAAKEIQGGGGGQPFFATAGGKN
                     PAGLDAALAKTLEFLE"
     gene            23957..24919
                     /locus_tag="PK502_02535"
     CDS             23957..24919
                     /locus_tag="PK502_02535"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPG99727.1"
                     /translation="MVKVKYHFNPHTLKFDAVSLPFYKRLAKILIHLGFYLLVFVVLG
                     YGFSMVFDTPIAQGLKRTNSEYSLKYEMALRQMQEFNDILTEIEQKDNNIYRAIFECD
                     SIPFSIRRGGYGGSERYSELVNNSNSHLLVKTFKILDELSWRAYIQSRSLDEVTELAI
                     NKERMIECVPAIQPISVKNLVRISDFFGYRRDPITRAPTMHEGLDFAGPIGTPIYSTG
                     DGIVIEAGYSFNGYGNQVMIDHGFGYKTRYAHLSKVNVKVGERVKRAEVIGKLGNTGK
                     STGPHLHYEVIVRGKPVNPINYFNDMTEQDYEAMLKNYSSQFLD"
     gene            24937..25908
                     /locus_tag="PK502_02540"
     CDS             24937..25908
                     /locus_tag="PK502_02540"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPG99728.1"
                     /translation="MAKYKYKFHPEQLTFVRVRATLKEKLFVALKYMVATAIVGIISY
                     LVFPFFIDTPEVRKLKRENQELMLNFELIDKRLDQLRAVIENLQQRDDNIYRIIFEAE
                     PIHPSVREAGMGGVDRYSYLEGLSNNKIIVETARKIDLLSKKAYIQSRSFDEISTLAK
                     RKEEMVRCIPAIMPINNKDLTRMASSFGMRIHPFYKVLKMHTGMDFTSPLGTEVYSTG
                     DGVVTNVEYAQRGYGYNIIVDHGFGYKTRYAHLGKIIVKPGQKVRRGMVIGLVGNTGT
                     SVAPHLHYEVIRNNTPVNPINYYFNDLTPEQYDKLVEIASQPTQSFD"
     gene            25926..26264
                     /locus_tag="PK502_02545"
     CDS             25926..26264
                     /locus_tag="PK502_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPG99729.1"
                     /translation="MPYKEKNIEKFYFSIGEVAEMLGVNASLIRFWEKNFDIIQPSKN
                     QKGTRFYTKEDIDNLKLIYHLVKEQRMTIEGARKKIAEDKHSVSDSFKIIESLNSIKQ
                     MLLEVRDQLD"
     gene            26292..>26920
                     /locus_tag="PK502_02550"
     CDS             26292..>26920
                     /locus_tag="PK502_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPG99730.1"
                     /translation="MKLDEIVSYFEELAPTAYQEEYDNAGLIVGDASMEVKAALLTLD
                     VTPEVVLEAIDKGANLIISHHPIIFRGIKKFTGSSNVEKTVAAAIKNDIAIFAAHTNF
                     DSMMGGVNLSIARKLGITNLEILQPINNGLFKLVTFVPVDSVEKVRDAMFKAGAGQIG
                     GYDCCSFGSEGFGTFRAGDNTHPYVGQKGEMHREPEVRVETILPKNILA"
BASE COUNT         7056 a         6022 c         6198 g         7644 t
ORIGIN      
        1 aaatgcaact tttttgttaa acaatatttg atatagcttc tctttgggtg ttaaaaatcc
       61 aaaacgcttt cttggcctgt tgttcagtat catttccacc cgttgcaaat gctcattttg
      121 aattatttgt tgaaagtatt gtttgcccaa gtaaaaaaat ctgtcatagt gaatactata
      181 atttctttgt tttgggtttc tgctgttgcg tatttttttt gttgaacagc gtctttcaac
      241 cacttaactg caccagtctt ccaacctgcg ccatcaatca aaatcattat tgtattttcg
      301 ggcattgctt caattgtgtt tagatacaag taaggtagtt tctcatcaac agaaccttta
      361 acttgttgcc acttgcactc aatacgaatt tgcaaatcga atctctttga aatgagtaga
      421 aattctgtat tgcctttgtg attgtaaatt gttgtaaatg gaacattctc aagtagcagc
      481 tcttctccgt atttctcttt gtttttatcc caaacacgat agttgataag ttcaaatcct
      541 tttcctgtaa gaacagtttt aactgccact tcaagttggt ttccagtaat attaccctta
      601 gctccttttt ccatttatgc aatttgatag tttgtaacaa cgagttcatt aactgctcca
      661 cgctttgtcg ggtcagaatt tatcattcgt ttagcaggaa ttctagtaat gttgtagcca
      721 cggtatatat cgtcaaaaaa attgtcagaa gggtcgtagt ttttgggatc tgagttgctt
      781 aacattagat gcgttccttt attgtcaagt tgcctaaata gttttgcaag ttgaatttgc
      841 tcattttctg caaagtcttg tttactgtat gctttgaaac tagctgtttt acttattggc
      901 ctgtaaggcg gatcaaaata aacgaatgat tttgctttaa tgtcgtttat aatttctttg
      961 aagtcagctt tcttgattac agcaattttc aaaacttgat gaactgctaa taaattttgc
     1021 tcgtcacaaa ttgtagggtt atcatagtca cctgctggag aattgaactc tccttttgag
     1081 tttactctgt aaagaccgtt gtagcaggtt ctgtttaaaa atattagttg tgctgctcta
     1141 ggaaaccatt gttctgaata cttatcaaag ttgatattgg agcgttgtaa attgaaattt
     1201 gccctttggt catagaaaaa tacttgacgt tgcttcttat cgagtttgat gtatttcttt
     1261 tgatattgat aaagaaagtc aagaagtttt gttacatcct tttgaatgac tttgtaagtc
     1321 aaaatcaatt cttcgttgat gtcatatagg taagcatctt caatatcata atgctgtgca
     1381 atgtcaaaaa aaactgcacc gctgcccaaa aaaggttcat aaaaagtttt aatctctttt
     1441 tgtttaagta gctttgggta gaattcttgg aatttgtcga gtagctgtcc ctttcctcca
     1501 gcccatttta gaaaaggctt tgccccaaca ttgaaatggt tgaaaaggtc ttcagatgtc
     1561 gaaacgttgt cgtccttaat tttatatttt atagcttgcg tcatagtgtt taaaaccatt
     1621 gtttaaattt agtcagaaaa aagagtaaag taaaaaaatt gagattatag tttcgaccaa
     1681 gcattttgca ctactcgttt atccccgccc ttcatatggc attacttcca acttgggaag
     1741 tatagacgct acgacattac attatttggt aaacaaattt atggactttt cttaaatcac
     1801 aaaaaatcta aaatatttcg acagatacct tctccgatgg cctgagcatg tgtctcgaac
     1861 ctgtagctga ggttaatggg tggttggtgt agttaaccta cgaccggtgc agctgcacca
     1921 ttgcccagcc ctcgcgggtt cgggttgcaa cgggctctag tcctatgctt ttagcatatt
     1981 gcagaagcct atctacgtcg gtggtgagta tgccgctaag gagcatatgt cccttgcaga
     2041 ccaggctctg agcgtaggcc ggcaggttgg caagcagcac gttcagggtt atgttggcca
     2101 aaatcaggtc gaattcccgg ttggccagca gctgcgcatc gccgagcagc acctctactc
     2161 tggagcagcc attaattctg atgttctcca gggtgttacg gtaggcccac tcgtcggtgt
     2221 cgatggctgt gacggaacgg gcttttctca gttcggccat tatggccagc acccctgtac
     2281 cgcaccccat atctagcacc cggcaatcct ctacattcat gtccatgagc gcttcaagca
     2341 tcatgtaggt ggtggggttg tgccccgtgc cgaatgccat cctggggtta atggttatgt
     2401 tgtattcagc tgcggggttt accgggtgat gtgaagccct gatgtggcac cggccatcaa
     2461 cagttaccgg ttcgaagccc gtctcccatt cctcgttcca gtccctttcg ccggtttcac
     2521 ctgcggagta gtcaatgctc acccctgccg gaatcgctat ggagtgaagg gtgttaatag
     2581 cagcgctaac actatgaata ctagaatacg ctagtagttc actttcgctc tccataaagc
     2641 tgtcaaagcc cagttcggcc agctgggcga tgattatctc attggtttcc tccgaaaagg
     2701 gagaggtttt aatgcggagc tccgtgaagg cagccatcag aacgagccag ctatggctat
     2761 aaaatcagca gcaacaagcg atgcgcctcc aatcagcccg ccatccacat cgggcttggc
     2821 aaaaagctcg gcggtgttcg agggcttgca gctacccccg tacagtatgc taatgctatc
     2881 ggccagtgtt gaaccgtatt tgccggccag caggccacgt atgaatgcat gcatttcctg
     2941 cgcctggtca gccgttgcgg tttttcctgt gccaatggcc caaacgggct cgtaggcaat
     3001 taccacctgc tgaacctgcc tggtttccag cccgaaaagc gattcggcaa cctgcttttc
     3061 gacaactgca aagtgctgtc cggcttcacg ttctcccagc ttttcgccgc agcagaagat
     3121 aggggtgagg ttttcctgaa gcgctagctg caacttttca aaaagcgagc ggttggtctc
     3181 gttaaagtac tccctgcgtt cggaatgccc aagaataaca tattcaaccc cagccgattg
     3241 cagcatggag gccgatacct caccggtgta ggcacccttt ggccattggg cacagttttg
     3301 agcacccacc cttacacggc tgcccctggc cagctttaca atctcgctca ggtgggtgaa
     3361 cggaggtatc accaccagct caaccccctg gtcaaccctc gatgagcctg caatcacctc
     3421 cttggccagg ctgaatcctt ctgccaggct ggtgttcatt ttccagtttc ccgctacaat
     3481 tttttttctc atggcatatt atttttaagg tttcccgtca acagaatttc cagacaaaca
     3541 tagcaaaaat tcctgtcagc ccattcttac ccttgggtca agcagggagt agataatatc
     3601 catgatgagg ttgaccacac cgaaaattaa ggagatggat attacgcatc cgatgaccag
     3661 tggaacgtcg tagctgttta aggctgtaac cataacaaat ccaagtcctt tccacccaaa
     3721 aatatattcc acaaagatga cacctgccat aagggaggca aaccatcccg aaattgccgt
     3781 taaaacaggg ttgagggagt tttttagtgc atgtcgcatg atgatgtgcc ggaactttag
     3841 ccccttggcc ctggccgtgc ggatgtagtc ctggtttagg gtatcgagca tggcgctacg
     3901 cataagctgg gtgatttctg ctagtggtcg gatacccagc gtaaatgcag gtagaatgag
     3961 gtttttaagc tgaagatgaa ccccctcgcc caggtcatca acctcccaaa ggttaccggt
     4021 aagattaagc ccggtgtagc tgccaagcag gtaagcaaaa acccaaccta ccagtattgc
     4081 agcaaaaaat gagggcaacg acatgccaaa cgatgatgtt atcagcagca tcctgtcaac
     4141 ggccgtatcc ttacgaatgg cagcaataat tcccatggca atgcccacga tggttgcaaa
     4201 tattatggct accagtgcca gtataaaagt gttgggaagg gtttcggcaa taatggaggt
     4261 taccggtttg cggctttggt aagaacggcg caggtagggg agtttgagta cgagctgtcg
     4321 gttgtcggag agttttacca ggttaacaat gccgctgtag agaagtgtat cgaggtatat
     4381 gtagcttttg gggttgttgg gattgtgaac cgacaggggc gacaggtcgt tgaggtactt
     4441 taggtactga acggcaacgg gcctgtcgag ccccatgtca gcacgtgccg ctgccagcga
     4501 ggctgaatcg gtgcgctgac ccatcaccat gcgggccgga tcgccgggaa ccatgtggaa
     4561 tagagcgaat atgagggtaa ccaccccaaa aaccaccagg atgaagtaaa gtattctttt
     4621 aataacgaag cggatcattt tgcgttgtaa atgaggagtg attaccttct ggttgatggg
     4681 cagcccaatg tttattgctg gttactcggt atcaaactgc ttcgactcct cccaaatggc
     4741 attcatctgg tccagcgtca tatccttaag cgattgtccc tgcttgatgg ttttagactc
     4801 caggtagcca aaccgtttca tgaacttgcg gttggtgcgt tccagcgctg tttcggggtc
     4861 aatattgtag agcctggccg cattgacaac tgaaaacagg agatcgccaa actcgccctc
     4921 aattcggttg cggttttgcc tttccacttc taccttcagc tcctcaagtt cctcctcaac
     4981 cttatcccaa acctgaccgc gctcctccca gtcgaaacca acggctctaa ccttttcctg
     5041 aatcctgttg gccttaataa gcgaggggag tgaggccgga acacccgaaa gcacagtttt
     5101 gttcccgtcc ttttcggtga ttttcagctg ctcccaattt tccactacct ggttggtatc
     5161 ctttacctca acattaccgt agatatgcgg gtgacggtaa attagcttat cgcaaagact
     5221 gtttataacc tcagccatat caaactggtt ctcctcagcg gccattttgg agtaaaagac
     5281 aatgtgcagc aggatgtcgc caagctcctt tttgattgcc tgaaagtttt tatccagtat
     5341 ctcctccgac aactcgtagg tttcctcgat ggtaagtggc cgtaaggttt cgaatgtttg
     5401 gctgcggtcc caagggcact tctcccggag ctcatccata atggtcaaaa gcctgtcgaa
     5461 tgcctctctt gccctctcgt ttcttgtgat gttatctgtt ttcttatcca tattgctaaa
     5521 tttattgaac ttgctagcgg ttagtgaact taaccataat gctcgaacct acagaaagag
     5581 cgagaagctc ggtcagaaat ccgttgcgaa tggcaatttc caagaggtta agcgagttga
     5641 agagagccaa catgtcgcca tcctctacat cggcgtagtt ttcaacaatt tcctcaattt
     5701 tgtagctgaa gctttgcacg tagatggaaa atttccggcc ctttcgcacc tgttcaaact
     5761 gctccctggt gatgttggtt attgcatttt ggtaggaatc aacatagata acgcttcccg
     5821 ttattgacga ttcgtcggat gctgccctga tgttaatgtt ctgcttgtag gtgccggcta
     5881 tcagcgcaat atcggccaag ttggagttaa aaagtagcat gttcatggcg ctggcaacag
     5941 cgctaagcga cccaaagctg ctatgttctt caaagggaat ggagtaaact ctgtccggag
     6001 caacttcggt aatcaggcca actatgccgt tgtcgggcac aatcatgaaa tgacccttta
     6061 gctcgaacag cagaagctgc ttgctggttg tgctatcgct gttgaccatg caaatatgta
     6121 tggttcccgg cggaaagttc tcaaagcaac cccgcatgat aaatgctgcc tggatgtaat
     6181 cgaatggcgg aaccctgtgg cttagctcca caatgcggag ggtaggctca atggagagca
     6241 acctgccgca tagaatgccg acatagtagt cgttttggtt ccagtctgtg gttaaggtta
     6301 ctatccgcaa attatccatg ggatttgcta aaagcattac aaaagtaatg gagtaatcgg
     6361 gaatattgaa aattagcatc ctatttcaat agcatcacca cgcaaatagc aagtatccaa
     6421 ccacataact atatggactg attttaaaaa agaccaggga atcattatga ataacgtagg
     6481 taaatcaaga agtgcgttgg aatgcctaaa accgaaccta gacgccaaac ggtaagaaaa
     6541 aaatcaatta tactaaaaac ctacactctt gcggaagtta cattagctaa aaaattgatt
     6601 gctgaaactt taaaaatggc tgtataaaaa tatttgcgcc aggggcaccc catttaacag
     6661 gaatgggtta tatttgtgag gttttggtta ttaacagtag cttgtttact aaattcaatc
     6721 tggttgagaa ctcaaatagc cttacaatgt tttgattata ttgcccagct cccgttgggt
     6781 ggcaatcttg tgaaatttta tggtaggaga gcggattata actgttgagg gagttgatcc
     6841 cctggccatt tacggggtga acaacacccg tttcgagatg atttcccgcc actaccccaa
     6901 gttaaaattg gttgccaggg gtaaccagat taaggtaacc ggcgacgagg agctgattga
     6961 tgacctggag agcaaaataa accgcatggt tgagttttac agccgatact cccgattaac
     7021 ggaggctaac atacttgatt ttctcaatga taacgacagc tcaattgtta aggccgagga
     7081 ggatgctctg gatgtgctgg tttatggtac aaacggccga actgttaagg cgcgaaccat
     7141 taaccagcga aagctggtgg agttatacca aaccaacgac ctgctttttg ccataggacc
     7201 tgctggttcg ggcaaaacct atacagcaat agctctggct gtaagggcac tccgaaacaa
     7261 ggaggtgaag cgaatagtgc ttacccgtcc tgccgtggag gccggtgaaa ggttgggctt
     7321 tcttcccggg gatatgaagg ataagctcga cccctacctt caacctcttt acgatgccct
     7381 acacgacatg ataccacaga aaaaactgat ggagtatatg gacgatggga ctgttcagat
     7441 tgctcccttg gcctacatgc gcgggagaac cctcgacaat gccttcgtta tactcgacga
     7501 ggcccagaac accaccctta accagctcaa aatgtttctt acccgaatgg gtaaaaatgc
     7561 caagttcatt gttaccggtg acatcaccca gattgacctt cccaacagag gtgattctgg
     7621 attggtaaag gcaattggaa ttttggaaaa cattggtagc attgccatgg tttactttga
     7681 cgcaagggat attgttcggc ataagctggt gaagcacatt gtgaatgcct ttgagaggta
     7741 cacctccggt aatgggagtt ctgaaaggac tgaaaataat gatgagtgtg aggataactc
     7801 tcagtagtaa ccttggttct agtgaagtgc catttgcaac agcattatct tctaaacact
     7861 aaacattgtg ggcgttgggg tgtttgccac caagaaactt tttggtaatc ctaatacact
     7921 gcactttaac ctcaatggaa cagaagccaa atcaactcaa actaaaatac agaagcaaat
     7981 ggaagcgatt gtaagaacag atttcaaatt ccctggccaa acctctgtgt accatggaaa
     8041 agttcgtgat gtttacaata ttgccaacaa gcacctgatt atggtggcaa ccgataggat
     8101 atcggccttc gacgttatcc ttcccaaagg cattccctac aaggggcaag tgcttaacca
     8161 aatagctgct aagtttctgg atgccacagc cgacatagtg cccaactgga aaatagccac
     8221 ccccgatccg atggtaaccg ttgggcgcct gtgtgaacca tttaaggtgg agatggtggt
     8281 gaggggctac ctcaccggtc atgcctggcg ggagtataaa tcgggtaacc ggttactttg
     8341 tggggtgcca cttcccgaag ggatgcgcga gcaccagaaa cttcccgctc ccataatcac
     8401 accaactacc aaggccattg aaggtcacga tgaggatatt tcacgtgagg aaattatcgc
     8461 cagcgggctg gttgaagagc atgactacgc acaacttgag cgctatacac tcgctctttt
     8521 tgaacgcggc acccgaatgg ccgaaaagat ggggctaatt cttgttgaca ccaagtatga
     8581 gtttggacgt gccgacggcc gcatctacct gattgatgaa attcatacac ccgattcttc
     8641 ccgttatttt tacgccgatg gctataccga aaggttcaac cggggtgagg agcaacgtca
     8701 gctttctaaa gaatttgttc gcgaatggct tatggccaac ggttttcagg gacaggaggg
     8761 acaaaaggtt ccggaaatga ccccagaatt tgtaaaccag gtttcggagc gctacataga
     8821 gctctacgag caaataaccg gcgagaaatt tcagagggca gagggttctg atgtaatgaa
     8881 gcgtatcgag cgcaacatac tcaactttat taaaaccctg gtttagcacc aactcctgct
     8941 ccagtagatt tttctgaaac catgttaacc ttcaacccct ttgctgaata gctttagctt
     9001 ggggttttat acctattcca gcagttcaag aatggcttcc ctgctaatct ggcctagcga
     9061 acttgacgag tttacgaaca gctgggcaag gttctccttt ctttcctgca tggagagaat
     9121 tttttcctcc acagtttcgg atgtaataaa acggtaaaca aaaacttttt tatcctgacc
     9181 aatcctgtgc gaccgcgatg cagcctgcat ttcggcagca ggattccacc atgggtcgag
     9241 aatgaaaaca tagtcggctg cggtaatatt caggccaact ccacccgctt taagcgatat
     9301 gaggaatacc tttttatccg ggtcggtttg gaacctttcc acaatttcgc tccggttggc
     9361 tgatgaccct gttaatttct cgtatcctat ttttctatcc tgaaggaaaa cctctacctg
     9421 ctcaagatgt ctgacaaatg aggagaacac caggattttg tgatgacctg cggtggcgct
     9481 ctcgatgccc atagccacct cgtcaaactt gccggaatcg gccagccggt attcgggcaa
     9541 catggccgga tggttggcaa tttgtctgag tcgggttagg ctagccagaa tggaaactgt
     9601 tgactgatta ggggctggac tgtctaaagt ttcgagtatg aagttgcgga caaccgattt
     9661 ttccttctcg taaatctcct tttgtgaatc ggacattctg cagtaaacca cctgctcgct
     9721 aatttcaggt aggtctttag ccacctcctc cttagtgcgg cgcaaaatga gaggctcaat
     9781 aagctgcttc agctgctttt ccttttcgtg atctccctgc cgctcaatgg gagtttgaaa
     9841 ctcattacgg aaggccctta agccgcccag cattcccctg ttcaggaaat taagctgcga
     9901 ccacaggtcg agcagggagt tttcaacagg tgttccgctt aggctgagat agttgatggc
     9961 cctcagctgc attacggagc ggtagccttt ggtcaacgga ttttttacgg tttggctctc
    10021 atccagaaca acatatcgaa aggttacctc acggaacatg tcaatatcgt tacggatgag
    10081 gccgtacgtg gtaaccacaa cgtgatggtt cagcaaccag ctggccgatt ttaccctttg
    10141 ctgccccatg taggcggcta ctttcagctc gggggtaaac ctgcccgcct cgctaatcca
    10201 atttgccacc agtgatgttg gcacaacaac caacgaggtt ttacccattc ctgtggcata
    10261 gttttgctcc tcattgattg attgtagcaa cgttaaggct tgtatggttt tgcccagtcc
    10321 catgtcatcg gccagacaac cgttgatgcc attttggttt agcagcttaa gccatgcata
    10381 ccctacaagc tggtagcttc gtagccgggc atttagccgt tggggtagct ggataccttc
    10441 ttttttaagg ttggaaagcc tgtgcctgag ctccgaagct gcgggagagt tgacttcatt
    10501 ggtgtcggct agcaggccaa aaagcgattt tggcaacctt atgccttcat tttcctcctt
    10561 gcctatcagc attaagggtt tgtaacggct gaaccatacc tccggtataa tgaaaacctg
    10621 cccgttgggc agcagaaatt ccctgatgtt tttgaggatg ttccgcttca ggtaaacaaa
    10681 tggaatttca aacccatcga gcttgatttt gccatacagg tcaaaccagt cggtggtgga
    10741 gtcggttgaa atggccacta caaccccgtt gaggtagaag ttcatacccc taaccagttg
    10801 ggcaacggca aaaccttccg ttttaagctg gttaacatgt tggttgagcc actccaccaa
    10861 ggggtataga ttttcctttg cattctcaac cgctgccggt aaaaattcgg cctcgctaac
    10921 ccgctgaagg ttaaactttt cggaaagtaa gcaaataact ctgctttccc atattctatt
    10981 tcggttaaag cggcgaaagg tgtacccgtt gccctcttca accagcctga cttcggtgtt
    11041 gtaaggggta ttgcataggt agcccctgtc gccatatgaa aattttagcg ttaggcacca
    11101 tcctcccgcc aacctctcct caagggtaag gattgccctt ccatcatgct gctcatcaac
    11161 aatattgaag ccaaccgcct ccacttgctg gtggcgtatg gcattcagca caaaagtttg
    11221 catgtatttc ttttcggccg attttggaac cgtgatgtag ggtttggata ggaatggagt
    11281 aagtttttta ccatcaattg cctcaaattg gtaaattgtt ctgtcaattc gaattatgca
    11341 gggattgttg gtaaccacct cgccgttggt agttgtgagg tttactgttc tgccgttgtt
    11401 catggccatg atggaataca ttatcgaatc aggggtgagt tcaaatctga acaggggcaa
    11461 ggttggctcg agttcaacat tcagacggtt ttcctcgtaa agattttttt cttccttttt
    11521 tcgcagaaaa ataggagtgt cggagtgcgc tgccagcgca acaaccttgg caattctgcg
    11581 ttcaacgtag gagtgaacat gactgtttag ctgttcactt cgggtcaggg agtccagaaa
    11641 ttctgccacc gattttgtct gtttgtcaaa gtacttctgc agggccttgt cggaaatttg
    11701 ctgcatctgc tgaagcatat ccttctgaat gggtgataca atgtcttcaa ggtctgatgg
    11761 ttgggctacc gctgcatttt cagaaatgat gaaaatacca tggctttcgg tgggtgttgc
    11821 gaggtaggct ccaatggttg ttccaagctg cctatggtgc tgaagggtaa gcgctaaaaa
    11881 tggtttgagg gtggccggca tagctacatt aatttttttg caattttagc ataaatcagc
    11941 caaaccttta gtttcaaact tataaggact aagagtaagg ttaaaggaga aggggataaa
    12001 aaaacttggc cagcttcgat ggaacctgtc cggctttgac gaattaaatg cgataaagga
    12061 taaagaattg agagcagata aggaagaaat gaataaatac ttatttagct gcttgatatt
    12121 tacttgttat cggttcgcac atcacggtaa aggaatgacc tgtgttaaat ttttagaatt
    12181 gaaaaccctc aacccctata gcttttccaa ttattaaagt cttatttttg tagaatatat
    12241 tttgaaattt aaacgttatt gaaactcttt gaattcctag gataatttgc atagctcata
    12301 ttgaattttg gctactcctt tttataaaat tccttactat tgaggattac gttaagtcta
    12361 aaacaatcgg attttaggag gtcataaaaa ggaatgcatt ttgttattga aggtggcaca
    12421 ggagtgtgtg cattctgcct tatatttctt aggtgtggag gataaccttc ttgctaggta
    12481 gatgtcagat tgattggtaa caggaaaata atatcaacat aaatattttt tagctgatga
    12541 gatcttacat tacggccttg cttttatgcc tatcacccat gcttcttgcc gggcaggtag
    12601 tggagggcga tataccctcc gttcaacgtt ctacagacaa ggtaaaaatt gacgggaaga
    12661 actactacat ccatatagtt aaaagtggcg aaaccctgta ctccattagc aaagcctatg
    12721 aggtttcaca gtcggatatt gctgtcaaca accccgatat ttacaacggg ttgaagattg
    12781 gacaagcgtt aaagattccg gtaagagcct ctgaaagtaa aggaaccgat gattacatat
    12841 accatattgt tgccaagggg gaaacgctgt atagcatttc cagaaggtac aacatccctg
    12901 ttgatgtgct cacaagcatt aaccccgagg taaaggatgg tctgctggtt agccaggtta
    12961 ttcttattcc tcgcaaccta atgatacccg aggcaaagaa gccggctgcc gacaccgtgc
    13021 agtttatcat gcacgaagtg cagaagggag aggggttgct ggccgttgca cgacgatata
    13081 acatcgatgc taaaaccatt gagacttaca atcaggaagt tctaaaggac ggccttaagc
    13141 taggcgagta ccttagaata cctgctgtaa agcagcaccc tgccgattcg cttatggtta
    13201 caagcaaacc agaaccggtg gcattctttt ctacctgcaa aaacaacttt aggtacaacg
    13261 gccaaccctt tgacatttcg ctacttcttc cctttagcca gggtagcgcc gtcgatagcg
    13321 acgagcttat cgatgaggcc gaagaggtca aaatggatca tggtactacc aacacccttg
    13381 aatcgtcaag aattacctcg caggaaactc ttgcctcctt aaacttttat gagggctttc
    13441 tacttgccgt tgactcgatg aagaaagctg gagtttccat gaatatcaac gttttcgaca
    13501 ctaaacacca acccgaagag gttacaagta ttctaaataa gggcaaactc gataagtcca
    13561 acattatcat aggtccactg tttgttaacg agatcaaacc agttgtggct tatgccgaaa
    13621 gaaaatcgat tcccatggtt tcgcctctct acagtgggaa tgaagtcata tcagaatacc
    13681 ctggtctaat atcggtgaac caaggatatg gcgaacagct ggaggttttt ataaaaaact
    13741 ttaagtttag cgatacctgc cactatgttg ttatttacga catggagaaa attcttacag
    13801 gtccaattca aaattttagc tctgcactca accaaagaat ccgcgaatcg ggtgttaggg
    13861 ttgactctgt tgttcatcaa acagcagcga tgaaatcgga agaggttcaa gctaagcttc
    13921 atagctacat gaaagccgac gggtgtaatg tagttataat cccctcgcag gatgagcctt
    13981 ttgtttccga tttgctgggg caactctatg cggttaagtc tttgaatggg cttaagatga
    14041 ttgtttatgg ccctgcacgc tggcaaaaga tgaaaaatgt tccacctgat tacctgtaca
    14101 aactgaatgc ccgtgtgttt actccatttt acgttgacta ttcaagagac gaggttaaac
    14161 gatttgttgc cagcttcagg gataggtaca gagctgaacc caaccagttt tcattcttgg
    14221 gttatgatgt gggcatttac tttatttcgg cgctgaaaac ctatggcccc gactttacta
    14281 aatgtttaaa agatttccaa aaggacatgc ttcagtccac ctttactttc gatgtggttt
    14341 cgaatgaatc cagacttagc aacacccacc aatttattat tggctacacc gatgattacg
    14401 accttgtgag gattatacct tgaaatggcc tgcagcttca ttgcgaacga atgagcgcat
    14461 ccagcttcag aataactttt tggttgtgta aagaatagcc ggaacattcg ttttactcca
    14521 gcatgttgtt gataggttgg aggttagatg ttttcctgta ttttcctcct agcgggaacc
    14581 tttccctaag cgtgtctggt ggttggggca gctggcaatt ttactgccta aaaagatgcc
    14641 taaaaatggt attgttaaac atcttttgtt taaggggtta cattttggcc attcgtaatt
    14701 atattgtatg tttggttcaa aattaaacta taaaaatcca ttaaccatgg ctaatctgga
    14761 cttatcggca tacggtgtaa accaacctgc cgaagttttt tacaatccga gttacgagct
    14821 tctttatcaa cacgaaaccg atccttcact ccaaggatac gagagaggca ttgtaaccaa
    14881 gttgggcgct gttaatgtgg acaccggcgt ttttaccgga cgctcaccta aggataagta
    14941 tgtggtgaag gagcccaata ccgaaaaaaa tgtttggtgg gcccagccag ggaagaaaag
    15001 ttccgacaac aagcccatca cccaggaggt atggaatcac ctgaaggata tcagcgcaaa
    15061 acagctcagt gggaaaaaac tctacgttgt tgatgcctat agcggtgcaa atgaggacac
    15121 acgcctttgc gtcaggtttg tcatggaggt tgcctggcag gcacattttg taaagaacat
    15181 gttcattagg ccaaccgacg aggaacttaa aaattttaaa cccgacttta ttgttctggt
    15241 agcctccaaa acctcaaacc ctaaatggaa ggagcagaac cttaacagcg aggtttttgt
    15301 agcctttcat cttaccgagc gcatggccct aattggtggc acctggtatg gcggagagtt
    15361 gaaaaagggt attttttccg taatgaacta tttcctgccc ttaaagggga ttgcctccat
    15421 gcactgcagc gctaacgttg atcaaaaaaa tgggaatgtt gccattttct ttgggctttc
    15481 aggaacagga aaaacaaccc tttccgccga tccccaacgc gcccttatag gcgatgacga
    15541 gcatgggtgg gacgacaacg gaattttcaa ccttgagggt ggatgctatg ccaaatgcat
    15601 agacctggac cccgaaaagg aacccgacat ctatgccgct attcgtcggg atgcactgct
    15661 cgagaacttg gatgttgaca gcgagggaaa tattgacttt cactctaaag ttaaaactga
    15721 aaacacccgg gtatcctatc caatttacca tatccaaaat attgttaaac cggtttcgaa
    15781 ggcaggacac gctaaacacg taatattcct caccgccgat gcctttggag tgctaccgcc
    15841 ggtatcgcgg ctttcgcccg agcagatgca gtaccacttt ctaagtggtt ttactgccaa
    15901 gcttgcaggc acagagaggg gagtggttga gccccagcca actttcagca gctgttttgg
    15961 ccaggccttc cttatgctcc atcccacggt atatgcccgt gagctggtta aaaaaatgca
    16021 gcttcacggc tcaaaagctt atctggtcaa caccggctgg attggcggac cctacggggt
    16081 tggtaaacgt attgacctgc cttcaacccg ggccataatc aacgctatcc tgaatggctc
    16141 cattgaggag tccgagtttg agctgctacc tgtttttaac cttcaagtcc cattaagggt
    16201 taacggggtt aacccacatt tattaaatcc ccagaagtcg tgggcaaatc caggcgattg
    16261 ggataaagca gccagaaatt tggctggaat gttcattaaa aattttgaaa attttaccga
    16321 taacgagtgg ggaaaaagcc tggttaaagc gggtcctcac atctaaactt actaagtaag
    16381 tttcggttta tacactactt gctgcctaac tccaccacat gactgattat gtatgggctt
    16441 gtggtggttt tatttgacac gggttttaat actcctggct agcaataagc tatgcctcag
    16501 gtttctaata atcaacagcc ttaaggtaaa tctcctatgg cttgtattga agatgtccta
    16561 attttggtaa tttgtgtgag cgattagcct agctcaaccc tgggcgtttt ataacatttt
    16621 ttaacacttc ccccttgctt tcaaatagct gacatgacca gctttaacct ataatttttt
    16681 ccatgaatgt ttcggtttaa aggtggtatg agtaactttt aacacatatt cttttgccct
    16741 cttaaagcaa taggcttatc tttgccttta tggaaatttt gtccaaaata gccattgtct
    16801 ttctggctta cctcatgggc tccattccta cctccgtgtg ggtgggaaaa attttttatg
    16861 gggttgacgt tcgtgagcat ggcagcggca atgctggggc gactaataca atacgggtgc
    16921 ttggccttct gcctggaatc gttgttttta ttgttgatgt tttaaagggt taccttgctg
    16981 ttaggctgct ttacctcacc gatttttaca tacctaaaac aggcatgttc attaatttcc
    17041 agctcattct tggaattgcc gccatgattg gacatatttt cccaattttt gcccagtttc
    17101 gaggtggaaa gggggtagcc gttctttcgg gggttgtttt tgccctgcat ccctatgcca
    17161 ctctgattgt tttcgggata tggattgtat cggttatcat taccggctac gtttcgctaa
    17221 gctccatgat tgcagggttt tcattcccac ttattttgat atttgtttac cgcaccacaa
    17281 atccctccct gatactcttc gcctttattc tggccatcct tatgctattc acccatcaaa
    17341 aaaatattga aaggctgttt aagggggagg aatcaaaatt tcacttgaaa aagaaaccac
    17401 ataacgagcc cgataagcct aaatagccag tttatgaatt actttatcgg ctttatttaa
    17461 cctaacctga atagccctat aaggcaaatg caaatttttt atgccccaaa tatcacggga
    17521 agcagctata ccctgcctga agatgaggca cgacactgcc taagggttct taggatgagg
    17581 gtgggcgacg aggttgagct ggttgacggg aaaggaaatc tttttaccgg caggatagcc
    17641 aatcccgacc taaaggagtg tactctggaa attgttcgga aagccagcaa tttcggtgag
    17701 cgccacttct accttcacat cgcaatagct ccaactaaga atgccgatcg ttttgaatgg
    17761 tttgccgaga aggttacgga aattggagtt gatgagatca cacctcttct ttgcgagcgt
    17821 tcggagcgca aatcggtaaa ttctgaaaga attgagagaa ttgtgatttc ggccatgaag
    17881 caatccgaaa aagcctatct acccacactg aatacaatga ctagcctgga gaagttggtg
    17941 gcaagcactc ctgttgattt tgttaagcta atcgcccact gcaacgagaa gggcataccg
    18001 catcttaaaa aaatggtaaa accaggcgat aaagtgctca ttctcattgg tcccgaaggc
    18061 gatttttctc ctaaggaggt ggattttgca ctcataaatg gttttagagc aatcagcctt
    18121 ggcaattccc ggctaagaac cgaaacggct ggtatcgtcg cttgtcatgc ggtaaacatg
    18181 ctaaacactg aagatttttc ataaatttac caccatcttc taaataaaat tggcttgaga
    18241 agactactag tgggcgggta ccgattttca tggttccaca ttgctgcagc attgctggga
    18301 ggagtggtgc tactttttat tgttgcccca attttgggga tgattatttc cacccccgcc
    18361 aagagcttga ttgaaacagc agctgagcag gaggtagtga ggagtatatg gctttcggtt
    18421 tgggtagcaa tggcggctac gctggtattt gcccttttct gcataccact tgcctacttg
    18481 ctggcgaggt ttgactttcc gttcaaaagg ttagtattgg gaattatcga tttacccatt
    18541 gttatacccc attctgcagc tggtattgcc gtacttgggc ttattgcccg tgatggtctc
    18601 ttgggacgat taacttctcc gctgggcatt gagctggtgg gctcaccggc tggtattgca
    18661 attgccatgg catttgtgag cgtgccattt ttgattaatg ccgcacgcga gggttttgcc
    18721 cttgttcccg agcgtctcga aaaggctgcc ctaagcctgg gcgcttctcc aattacgatt
    18781 ttttttactg tggcgcttcc ccttgcatgg cgtagcgtgg tgtccggact agttatgatg
    18841 tttgcacgcg gtatgagcga gtttggcgcc gtggtgattg tagcttatca tcccatggtg
    18901 gctcccgttt taattttcga acggtttggt gcttttgggc ttaagtatgc caagccggtt
    18961 gccgttttgt ttataggtgt tagccttacg gtgtttatca tcatgcgact tctttcacgt
    19021 aacaggtcga accatgctta gggttgaagg ggtttgcaaa aagtttcagg attttagctt
    19081 gaaccagatt tcgttcacgg ttgaccaagg ggactacttc atgctgttgg ggccatcggg
    19141 ggcaggaaaa tcgttaattc tcgaaaccat tgctggactg cacaaaccag actccggaaa
    19201 aatatttttt cagggtgtcg atattaccca tattggggtt agggagagaa attttggaat
    19261 tgtgtatcag gattatgccg tttttcccca ccttacagtc tacggcaaca ttgcctatcc
    19321 gcttcgccgg aaaggtataa ggggtaagga gttgcataag caggtaacaa ggtttgccga
    19381 aagggtttcg gtagcgcatt tgctagccag gcatcctcaa actctttcgg gaggggaggt
    19441 gcagcgaact gctctggctc gtacattggc catggagcca cgtatcctgc ttctcgacga
    19501 acctctttcg tctgttgatg ttaagcttcg gtctgagcta cgctccctgc ttagaagcat
    19561 aaatagcagc ggccaaacgg tgattcacgt tacccatgac tacgaggaaa cccttgcgct
    19621 tgcatcccac ctggcagtag ttaacggggg ttgcattgag caggttggaa gtgttcagga
    19681 gatttttcag aatcctcgct cggagtttgc tgccatgttt gccggagttc ataactttta
    19741 ccccgctagt attgtagaat tttcggagca tggattaaca atggtcaggt tgaacgacac
    19801 tattgctata tgctgctaca ccgaaggttg caatggagag ggctttgttt gcttttccga
    19861 ggagtccgtt atcctctcaa ccgagaaacc gcatcagagt gctcttaatg tttttcgggg
    19921 tagggtagtg gaaattttcc cgcagcgttt tggcctaatg gctgttgttg atgctgggtt
    19981 tagggtgctt tcgaaagtaa cctgggaatc ggcaagccgt ttcggtctta cccccggtgt
    20041 tgaggtgtgg gtatcgttca aggcaagttc agtaaggttt atccctaggg ccagctaatt
    20101 tacctgggcc tgctatatga tgaaattttc ctgctattct ggtaaaccat tgaaacatac
    20161 tgaatgccaa aaaatatcaa caggaatacg atggcgtagt agtagagcga aagtgtgagg
    20221 aaaaaagtcg ctgcaaaaat aaaaacgctc cagagcaaat caacaaaagt aagaaccact
    20281 ccccatagaa tcccgctttt aataaccgtt ccgagtaaat caaccaaaat gcttaccagc
    20341 aatgataggg cttgacacca aactacccaa tctattatgt cgggattgtt tggaatagcc
    20401 ctaatgccat gttctatgct gttaagtagt aaatccttcc agttgatgta tagaattgcc
    20461 aggagcaatg cgttgataaa aaaaaccacc ggcattagta caagcccctt tacctgaaaa
    20521 tcatgttttt gggccagtcg gtttttatac tgaacgatta gcttgcctga aaaaatccaa
    20581 atcacgattg caggaatagc tattagccag aacacccacg attcccagct ggctgttttc
    20641 tcaacccagc ttaggagatt cggagttggt gtgggttcaa tccggttgag gtaggaagct
    20701 gcaacatatc cctttttagt gccaacctta accgtcaccc attcggtatc cgagacaata
    20761 gcatttatcg tatcgccctg ttgaaggggg acaactgggg cagaagcggt tgagggggag
    20821 ctgcgaacaa ttaacgtttt ggcctttacc acgtagcgtt ctcgaatgtt atagctcccg
    20881 ggtttcaggc atgaggcaac caccaatggt aacaggagga aaataatgca cctaacccca
    20941 ctttgcctca tagtatagaa agttgaatcc agcacaaagg taatgtctga agcaaatgca
    21001 gcaaaaaaat tagtttaaaa aataaaccta atggttaaag gggaagcggt tctactgttt
    21061 gctagtcatt tcattaaaag ctaaacaccc ttcagaaggt aacaggataa gttcttcttt
    21121 tttcttaaag ctgaaagcta tgcttagtct atgttaacac ctaagtaaaa ttaagagtct
    21181 aaaaatctta ctccaggaat tctaatgttt tggccaaagc ggcatccaga cctgccgggt
    21241 ttttccctcc ggctgttgca aaaaagggtt ggccaccacc gcccccttga atttccttag
    21301 ccgcctcacg aataatgctt gttgcgttta gctgcaaaga ttggacaagg ttatcgctta
    21361 tcataactgt tagaattgcc ttttcgcttg ccgttgcccc gatagccagg aaaaggtttg
    21421 gaacttcatt ccttagctga aaggctaaat ccttgatggt agttgaatcg ttcaggccta
    21481 ccttttggca aataacgttg attccgtttc gctgttcaat actttgctta agctggtgct
    21541 taacttcggc caacttttca gcctgcaggc ttgaaatctc ttttcgtagc atttcgttat
    21601 cggctaccat acgggcaacg gtttgggata cattggaggg gtttcccaac aaggacttaa
    21661 tctccttaat ggtatcctcc tgagagtaaa caaactcctc cgcttttatc ccggtaatag
    21721 cttcaattcg ccttactcct gcagcaatag cggtttcgga taaaatcttg aaaaatccta
    21781 ttctacctgt tgcttcaaca tgtgtgccgc cgcacagctc aacggactcg ccaaaatgga
    21841 taaccctaac ccggtcgcca tacttctcgc caaaaagcgc catggcaccc atacttcggg
    21901 cttcgtctat aggcatgttc cttttctcat caagatgaac attttcacga ataacgctgt
    21961 tcaccagtct ttcaacctgt cggatttcgt cctcggacat tttctggaag tgcgaaaagt
    22021 cgaaacgtag gtagtcggga tgaaccagcg agcccttttg ctcaacatgt aatcccagta
    22081 tttttcgtag ggcgttgtga agcaagtgtg tagctgagtg gttggctgct gtgcttgccc
    22141 gtcggttgac atcaaccgat gccacgaatg tgccgttagg gttggccggc agcttgtcaa
    22201 caatatgtat cgtcaggttg ttttccttaa ttgtgttcaa caccctgata gcttcgccct
    22261 cgccggttat ggttcccgag tcgcctacct gtccgcccga ttcggcgtag aatggggtct
    22321 tatccagcac cagctggtac tgttcgtttg actttaccct tacggtgcgc atcctggcaa
    22381 tatgtacgtt acaggtgagc gtatcgtagc ctacaaactc cgcattttcg tcatcgttca
    22441 ctacaatcca atccatttcc tctaccgcgg catcggctct ggagcgttcc ttctgcagcg
    22501 ccatttcgcc atcaaacccg gaatgatcga ccgacaggcc attttccctt agtattagct
    22561 cggttaagtc gagtggaaat ccataggtgt catagagaac gaaggcgtct tttcctgaaa
    22621 tttccatttt gtgctcttta cgggcgcgct caaccagctg gtcgagcatg cgcattccgg
    22681 tttcaagtgt tctgaggaaa gctgtttcct cgctggtaat gacacttgta ataagcgatt
    22741 gctgtttcct aagttcagga tactggtcgc ccatctgctc ggttagtacc ggaataatac
    22801 ctgccatgaa aggctccctg aagttgagaa atgtgtagcc atatcgaaca gcccgtctta
    22861 gtatgcggcg aatcacgtag cctgccttaa cattcgatgg gagttgacca tcggctatgg
    22921 caaagcagat agctcttagg tggtcggcaa ttacccgcat ggccacgtcg cttttggcat
    22981 cagcaccata ctttaggccg cttatgttcg atatgcggtc tatgagcggt ttgaaaacat
    23041 ccgtatcgta gttgctcttt ttcccttgaa ccaccattga taggcgttca aaacccatac
    23101 cggtatctac gtgctttgcg ggtaggggtt caagctggcc attagccttg cggttaaact
    23161 gtataaaaac caggttccat atctcaatta ccagcgggtg gccggtattc accatctttt
    23221 ccccggggtt agctttccgc tcatcttcac cacgcaggtc tatatggatt tcggtgcagg
    23281 gaccacaggg tccggtttcg cccatttccc agaagttatc cttcttgttg ccctcaataa
    23341 ttcgattaga gggtaggtgt tttgcccagc agtttaacgc ctcatagtcc ttttcaacac
    23401 cctcatcggg gcttccttcg aaaattgtag catacaacct ggtgctgtca attttaagta
    23461 catctaccag aaattcccat gcccagtcaa tggcttcgtt cttgaagtag tcgccaaacg
    23521 accagtttcc caacatttcg aacatggtgt ggtggtatgt atcatgaccg acctcttcca
    23581 agtcgttatg ttttcctgaa accctaaggc atttctgcga gttggccact cttttgaatt
    23641 ttgccggagt attgcccaga aaaatatcct taaactggtt catgccggcg ttggtgaaca
    23701 taagggttgg atcgtttttc accaccattg gtgcagaggc aacaatggtg tgcgatttgc
    23761 cttcaaaaaa gtttaaaaat gcccgacgaa tttctgcaga agtcataaaa tgcttgcttt
    23821 gaaaaggtta acatttatta attaatcatt tttcaaaaaa aaaattattt ttggcattga
    23881 aaaattcatt ttttaccata gatttgttgc atcttatcaa aaaggaggca aacatagtta
    23941 caaaagttaa aaaaggatgg taaaagtaaa atatcatttc aaccctcata cccttaaatt
    24001 tgatgctgtt tccctgcctt tttataagcg gttggcaaag atactaattc atttgggatt
    24061 ttacctttta gtttttgttg ttttggggta tgggttttcc atggtgtttg atactccaat
    24121 agcgcagggt ttgaagcgca ccaattctga gtactcgcta aagtatgaaa tggccttgcg
    24181 gcagatgcag gagttcaatg acatacttac tgaaattgaa cagaaggata acaacatcta
    24241 ccgggcaatt tttgaatgcg actccattcc gttctccatt aggaggggtg gttacggggg
    24301 ttcggaaagg tattccgagc tggtaaacaa cagtaactcg catttgctgg tgaagacttt
    24361 taaaattctt gatgagctgt catggcgggc ctacatccag tcccgttcac tcgacgaggt
    24421 taccgagttg gccatcaata aagagcgaat gattgagtgc gttcctgcca ttcagcccat
    24481 ttctgttaaa aatctggtta gaatatccga cttttttggc tataggagag acccaattac
    24541 ccgtgctcca actatgcacg agggtttgga ctttgctggg cctattggaa ctcccattta
    24601 ctcaaccggc gatggtattg ttattgaggc cggctactcc tttaatggct acggtaacca
    24661 ggttatgatc gatcatgggt ttggctataa aacccgctac gcacacctta gcaaggtaaa
    24721 cgttaaggtg ggtgaaaggg ttaaacgggc ggaggtgata ggcaagcttg gcaataccgg
    24781 gaagagcacc ggtcctcatt tacactacga ggtgattgtt cggggtaaac ccgttaaccc
    24841 gataaactac tttaacgata tgaccgagca ggattacgag gccatgctca agaattattc
    24901 tagccaattc cttgattaac tatctacatt ggaaaaatgg caaagtacaa gtataaattc
    24961 catcccgagc agctaacatt cgttagggtt agagctaccc ttaaagaaaa gttgtttgtt
    25021 gccttgaagt acatggtggc aacagctatt gttggtataa tatcctacct ggttttccct
    25081 ttctttatcg atacccccga ggttcgaaag ttaaagcggg agaaccagga gcttatgctt
    25141 aactttgaac ttatcgacaa gcggttggat cagctcagag cggttattga aaacctacag
    25201 cagcgcgacg ataacattta ccgcatcatt ttcgaggccg aacctattca tccttctgta
    25261 agagaagcgg ggatgggcgg tgttgacagg tactcctacc tcgaaggcct ttcaaacaac
    25321 aagattattg ttgaaactgc ccggaaaatt gaccttttaa gcaaaaaggc ttacattcaa
    25381 agccgttcgt tcgatgagat ttccactctg gctaaaagaa aggaggaaat ggtacgctgc
    25441 attcctgcca ttatgcctat aaacaataaa gacctcaccc ggatggcctc atcgtttggc
    25501 atgagaattc atccattcta caaggtgttg aaaatgcaca cgggcatgga ttttacatcc
    25561 ccgttaggca ccgaggttta ctcaacaggc gatggggttg ttaccaatgt ggagtatgcc
    25621 caacggggct atggctacaa cattattgtt gaccacggat ttggctacaa aacccgctac
    25681 gcccatttgg ggaaaattat tgttaaaccc ggccaaaaag ttaggcgggg catggttatt
    25741 ggcctggtag gaaacacggg aacttctgtt gctccccatc ttcactacga ggttatccgt
    25801 aacaacacgc cggttaaccc aattaactac tacttcaacg accttacacc cgagcagtac
    25861 gataagctgg ttgaaatagc atcccagcca acccaaagct ttgactaaac gattcttttg
    25921 aactaatgcc ttacaaggaa aaaaatatcg aaaaattcta cttttccatt ggagaggtag
    25981 ccgaaatgct cggcgttaac gcttccctta tcaggttctg ggaaaagaac ttcgacatta
    26041 ttcagcccag taagaatcag aagggtacga gattctacac caaggaagat attgacaacc
    26101 ttaagctaat ttaccatctg gtaaaggagc aaaggatgac cattgagggg gcgaggaaaa
    26161 aaattgccga ggacaaacat tccgtatccg atagctttaa gattatcgaa agcctgaact
    26221 ccatcaagca aatgctactg gaagttcgcg atcagctcga ttagccaaat tcttcaagtt
    26281 tccaaaccca aatgaaactc gacgaaattg tttcgtattt tgaagagctt gccccaacag
    26341 cctaccagga ggaatacgac aacgctggtc ttattgtagg cgatgcctca atggaagtaa
    26401 aggcagctct tctcactctt gatgttaccc ccgaggttgt tcttgaggcc attgataaag
    26461 gagctaacct gataatctca catcatccta ttatttttag gggtattaaa aagtttaccg
    26521 gcagcagcaa tgttgaaaaa acagtagcag cagccatcaa gaacgatatt gccatttttg
    26581 cagcccacac caacttcgac agcatgatgg gtggagttaa cttgtcaatt gcccgtaagt
    26641 tgggtattac caatctggag attttgcagc ccataaacaa tgggcttttc aagctggtaa
    26701 cctttgtccc tgtcgatagc gtggaaaagg ttagagatgc catgtttaag gccggagccg
    26761 gtcaaattgg aggttacgat tgctgttcct tcggctccga gggctttggg acattccgcg
    26821 ctggcgacaa cactcacccc tacgtgggcc aaaagggcga gatgcacagg gaacccgagg
    26881 ttagggtgga aaccattctt ccaaaaaata tccttgcaag
//
LOCUS       DAOPHX010000018        25475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_1958_length_25475_cov_5.957671, whole genome shotgun sequence.
ACCESSION   DAOPHX010000018 DAOPHX010000000
VERSION     DAOPHX010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 25475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25475
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1958_length_25475_cov_5.957671"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PK502_02555"
     CDS             complement(<1..374)
                     /locus_tag="PK502_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPG99731.1"
                     /translation="MKISYSWLKQYVDTDLAPERVSEVITSTGLEVDSMERIEAIRGG
                     LAGVVVGEVLTCDKHPDADKLSVTTVDVGEQKPLCIVCGAPNVAKGQKVLVATIGTTL
                     YFSGGEVTIKKAKLRGISSEGM"
     gene            960..2318
                     /locus_tag="PK502_02560"
     CDS             960..2318
                     /locus_tag="PK502_02560"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPG99732.1"
                     /translation="MKILKFGGTSVGDAQRIRKVAQLLPEAEPCIVVLSAMAGTTNAL
                     VEIANHSSSGNVSMAIEQAKQLEARYMETSHELFNSPVFRNRGEKFTNDMYAELYRKL
                     GCPPTKKVTLEIVALGEQLSTGLFHLYLTENRIRSTYLPALEFMRIDREREPDYFYIS
                     QSLERLIKANSFTRVFITEGFICRNAKGQTDNLGRGGSDYTAAIIGNVADSEEVQIWT
                     DIDGLRNNDPRHVVNTKPIRHLSYGEAAELAYFGAKILHPSTIQPCSEKNIPVVLKNT
                     LQPDDPGTLIDSVYIPKGIKAIAAKKGITAITIQSSRMLLAYGFLSHVFEVFNRYKTP
                     VDMIATSEVSISLTIDDCSNLQGIANELAEFSTVECTYDQTIVCIVGDFGANRKGYAA
                     RIFNALHEIPIRIISYGGSSNSISILVDSQSMTRTLQLLQEVVDTDVDVNLEQTANTI
                     SY"
     gene            2325..3485
                     /gene="lysA"
                     /locus_tag="PK502_02565"
     CDS             2325..3485
                     /gene="lysA"
                     /locus_tag="PK502_02565"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPG99733.1"
                     /translation="MKTPYYQYDLQLLRQTLDAATREANKYGYTLHYAVKANANPVIL
                     NEISSYGLGADCVSGNEISAALSGGFNPKGIVFAGVGKTDEEIELAIARGIYCIHCES
                     VQELMTIQKIASAMQTTANVALRVNPNVDAHTHKHITTGTMANKFGLTVEEARRIIKN
                     SDRYSNLNLFGLHFHIGSQITEMNVFEELAQRVNELQHDFYANGKPAYLNLGGGLGIN
                     YQNPDSELIPPFMNYFDVFARNLNRNSWQSIHFELGRSLVGQCGKLVTRVLYTKKMGS
                     KNFAIVDAGMNALIRPALYGAYHRIVNTSTSSSKMERYEVVGPLCESTDILGTNVLMP
                     VTQRNDVLEVLSCGAYAEAMSSRINLRDIPDYCFTNQLQEKTITKTGAPLQG"
     gene            3694..3837
                     /locus_tag="PK502_02570"
     CDS             3694..3837
                     /locus_tag="PK502_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99734.1"
                     /translation="MRKPLILLFVVVTLSLASCYKNNATFTVQVNDYAPTYTVAYTAQ
                     NGV"
     gene            3838..3996
                     /locus_tag="PK502_02575"
     CDS             3838..3996
                     /locus_tag="PK502_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99735.1"
                     /translation="MGSLKVPKNYEFFNEYSYGWQLNLKVKAEDSIFVYVQERGNLKE
                     PIISVRIH"
     gene            4369..5454
                     /gene="carA"
                     /locus_tag="PK502_02580"
     CDS             4369..5454
                     /gene="carA"
                     /locus_tag="PK502_02580"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPG99736.1"
                     /translation="MNKQKVKLVLADGSEYSGYSFGYFGSTAGEAVFNTAMNGYPESL
                     TDPSYRGQVLIATFPLVGNYGVPEGITEGNLHRFWESDRVQISGLVVSVYNEDFSHWN
                     AAKSLGDWLAEYRIPAIAGIDTRALTRKIREKGSMLAKILALNDVEWHDPNSENLVAQ
                     VTVAQKEIYGNGEYRVVLVDCGVKNNIIRCLTQRNTTVVRVPYDYDYTDEDYDGLFIS
                     NGPGNPKMNTATIAVLQKAMKSNRPIMGICLGNQLLALASGADTYKLPFGHRSHNQPV
                     ILKGTNRCFITSQNHGFAIDSSTLSDDWDVLFENANDGTCEGIRHKSKPIFAVQFHPE
                     ASGGPNDTEFLFDEFLQLMVKHKQINK"
     gene            5459..8692
                     /gene="carB"
                     /locus_tag="PK502_02585"
     CDS             5459..8692
                     /gene="carB"
                     /locus_tag="PK502_02585"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HPG99737.1"
                     /translation="MNSKYHKVLVLGSGALKIGEAGEFDYSGSQALKALREEGVKSIL
                     INPNIATVQTSWGVADRVYFLPVTPYFVERVVEKERPDGIFLSFGGQTALNCGIELFK
                     TGFLAKHNVQVLGTPVETIINTEDRELFARQLSQINVSTPRSQSARCLDDALQAAEKI
                     GFPLIVRSAFTLGGQGSGFCSNAEELRELSINAFSYSPQILVEESLWGWKEVEYEVVR
                     DRFDNCITVCNMENLDPLGIHTGESIVVAPSQTLTNAEYHKLRSLSIKIIRHLGIIGE
                     CNVQFALDPKSEDYRVIEVNARLSRSSALASKATGYPLAFIAAKLGLGYGLHQLKNPV
                     TAITTACFEPAMDYIVVKIPRWDLNKFSGVSHEIGSSMKSVGEIMAIGRSFEEAIQKG
                     LRMVGQGMHGLVANTKLNFPNLEEALVHPTDMRIFAIAEALELGWTVEKVHELTNIDR
                     WFLGRMQGIITLEKKLMQVASVEMLDRETLLEVKQFGFSDFQLARLIYNPGPDDIETR
                     QQVVRYHRIQLGIVPVAKQIDTLAAEYPSETDYLYLTYHGIENDTSPNQSNRTIVVLG
                     SGAYRIGSSVEFDWCTVSAAGKIRKLGYRAVMINFNPETVSTDYDMCDKLYFDELTFE
                     RVMDIIELEKPIGVMVSMGGQIPNNLAMRLHNAGVKVLGTSPESIDRAEDRQKFSEML
                     DMLHIDQPRWKQLSTLEEVERFVDEVGFPVLIRPSYVLSGSAMNVVSNADELRHYLVL
                     AANVSKTHPVVVSEFIEGAKEIEFDAVANNGEVMFDAISEHIEFAGVHSGDATLVFPP
                     QKLYFETIRRIRKIARAIAKELMITGPFNMQLLALNNEVKVIECNLRASRSFPFVSKV
                     LGFNFVEAATEYMLGRTPGYLPPSMFELDHVGIKASQFSFSRLSKADPVLGVEMASTG
                     EVGCLGTDFDDALLKSMLSVGYRVPQKSILVSSGPLRSKLALVEPLRLIERCGITIYA
                     TRGTAKFLAENDVNTIPVAWPDEDSQPSSIELIGSKQVELVINIPKNLSREELNNDYD
                     IRRATVDYNIPLITNARLAAAFLMAICNKNINTLDLRSWDEFFETKQ"
     gene            complement(8746..9621)
                     /locus_tag="PK502_02590"
     CDS             complement(8746..9621)
                     /locus_tag="PK502_02590"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HPG99738.1"
                     /translation="MRKVIFCIIVCLTAGFSFAQDKNAYTIFDSKGNVASYSDLVNRS
                     AATDVVFFGELHDNPIAHWLELELAKDLFRAKNGSLIMGAEMFEADNQLILDEYMSGL
                     ISMKRFEDEAKLWNNYQTDYKPLVEFAKESGVRFVATNIPRRYASAVAGGGFEVLNSF
                     PDEARKFFAPLPVAFDPELPGYKAMMGMDNMHGAGMQASQNLPKSQALKDATMAHFIM
                     ANFKEGSTFIHYNGSYHSDNKEGIVWYINRTNPNLRVVTVATVMQTDVDKLDAANLGI
                     ADFIIVVPESMTRTY"
     gene            9716..11524
                     /locus_tag="PK502_02595"
     CDS             9716..11524
                     /locus_tag="PK502_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPG99739.1"
                     /translation="MEITRTFDILEAGKETYENRPVLASRQNGTWEEYSIDKYSQIAH
                     NLSYGLMALGLRPGDRVATVSGNRPEWNFVDMALSQAGMVHVPIYPTISLDEYRYILK
                     HSGVKMIFAGDKSIYGKLQPLTGSLENFIGMYCFQNNNELPSWKEIAEKGKSVANSYI
                     DELFRIKKSISADDVVSLIYTSGTTGISKGVMLTHNNFLSNVRACTGLFPLEPGDKAV
                     SFLPLSHVFERMVNYYYQSIGLTVYYIDNLANLGNVISEVKPQIFMTVPRMLEQTYNK
                     IIAVGKDLSGLKKQVFFWAVNLGYHYNENGNSQYYNMKLRLARKLVFEKWQEAMGGKI
                     KLIVSGGAPLQPRLMRIFWAAGMPVYEGYGLTETSPVIAVNHSATPGNVRFGSVGPVL
                     SNVEVKISNDGEILMRGPSLMKGYYNAPELTSQAIDSDGWFHTGDIGHLDEGKFLTIT
                     DRKKEIFKLSNGKYVAPQLIENKLKESFFIEQCMVVGENEKFVSVLISPNFSFLHDWC
                     SHHKIHFQNNQELITLPEVISRFQKEINGINKQLSDYEQIKRFRLVPEQWSQETGELS
                     PTLKLRRKLLYDRYEKLLEDIYAHPKLVEIRGTNGD"
     gene            complement(11502..12581)
                     /locus_tag="PK502_02600"
     CDS             complement(11502..12581)
                     /locus_tag="PK502_02600"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPG99740.1"
                     /translation="MFFWVLPGVAVAQARPTSLQKPNGTAIIITGAAAKIAQEAALLQ
                     NLYEKGMLKNVVFISGASSGALNAVMLNAILEGKLTWKDYKSILGQLSNEKVFKPDGN
                     RLPVNTTPLRNTISSIVCDRLGYRILSDLPIPTSISVVNFKIAPFSDRTYRLCNLKIN
                     PESDPSLNLVDVLMASTSIPVIFPPSPIKNVRTIPNVPYYDGGIAADHVPYQALVEYE
                     KFRGVPVEKVIIVSRKRDTLSVAEELKQLGVERLGLVERFNISPDAITNRSFFNRLAA
                     MQKEAPELASKTSVFVPDFEHAYCMFNFNKLQEQYETTYSWGKHHDPIPLSDYLGRAG
                     VKPVAEVKPQGQLFNFRVISRHLFP"
     gene            complement(12814..13590)
                     /locus_tag="PK502_02605"
     CDS             complement(12814..13590)
                     /locus_tag="PK502_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPG99741.1"
                     /translation="MSSTTLVALIIIGLVTGVVTGLTGSSGVMIVVPLVNMLLGIPIH
                     ESIGISLIVNVIAPIAISYTYFKNGNIDVRVGVWIALGSIFGAQIGAFFADQIPETGL
                     GGSFGIFIILMGILICIKGFRGKKLESTKPVLEFKTEKRKNMAILMLGFAIGIISGVF
                     GAGGGGMILLILVFVLNFPIRVAIGTSSLLMAITSFSGALGYFIHGNIRLVPGLVMGS
                     GAALSGVVSARFANRVNEDILSIAVGVVFFMLGTLMILIA"
     gene            complement(13770..14930)
                     /gene="dnaJ"
                     /locus_tag="PK502_02610"
     CDS             complement(13770..14930)
                     /gene="dnaJ"
                     /locus_tag="PK502_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPG99742.1"
                     /translation="MTTKRDFYEILGVSRNATKDEIKKAYRQMALKYHPDKNPGDKEA
                     EEKFKEAAEAYEVLGDDTKRARYDQFGHSGVGNGGGGGGFTHDWTIDDIFSQFGDIFG
                     GHFGSFGGFGGFGNSRANQRATSRGSDIRVKVKLNYSEIANGVEKKLKVNKYVTCHSC
                     NGSGAASPSDFQTCSTCRGSGYVTRVTNTLLGQMQSTAPCPTCNGEGKTITKKCTECN
                     GEGVVRDNEIITIKIPAGVAEGMQLSVSGKGNAARRGGVPGDLLVVIEEEKHPQLIRD
                     GNDLIYNLTISIPDAVLGAPAEIPTVDGKVKIKIEPGTQPGKILRLKGKGLPEVNSYG
                     RGDLLVAINVYIPKTLTREERALFEKLAKSSNFTPNPKESSPIFDRIRGYFD"
     gene            complement(14944..15564)
                     /locus_tag="PK502_02615"
     CDS             complement(14944..15564)
                     /locus_tag="PK502_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPG99743.1"
                     /translation="MSKSSKQSKAAEDEKELETQQQVDIPEAEDKISAEGAEPNAAPA
                     SENVMEEADATDKALVEKLQKELEELKDKHLRLSAEFDNYRKRTLKEKSDMLKYSAEV
                     VLKDLLPVVDDLERALQAIESTDEITAVKEGLSLIVSKFNDFLNSKGIKEIEAMGSEL
                     NTDLHEAVAKTPVQDESQKGRVVDVIQKGYMLHDKVMRFSKVVVGE"
     gene            complement(15931..16335)
                     /locus_tag="PK502_02620"
     CDS             complement(15931..16335)
                     /locus_tag="PK502_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99744.1"
                     /translation="MQKFIRETGVLIAILVLCGSSCDELDSTNTITGVWRSREIYPGN
                     SYRTYNVSMEHYDQLDSTSYIIYNLYNLGLEVETYVQLNDTIFNILGCNSDSYIISGK
                     GYFHPLSFTIDWEYSVSGIDNDPFVMAHFEKP"
     gene            complement(16371..18386)
                     /locus_tag="PK502_02625"
     CDS             complement(16371..18386)
                     /locus_tag="PK502_02625"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPG99745.1"
                     /translation="MTREEFQKEILQGIPDELPALQPWDPDVNHAPKRKKILTLEEKK
                     LAVKNALRYFPPKHHAVLAAEFAKELEEYGRIYMYRYRPGYKIFARTINDFPHKSLQA
                     ASIMLMLSNNLDTAVAQHPHELIIYGGNGAIFQNWAQYRLTMKYLAEMTDHQTLVMYS
                     GHPMGLFPSFPDAPRVVITNGMMVPNYSSKDDWERFNALGVTQYGQMTAGSYMYIGPQ
                     GIVHGTTITVINAGRMHSKPGEKALGGKVFVSSGLGGMSGAQPKAAVIAGVVGVIAEV
                     NPKAVEKRYQQGWVNEVYTDLEKLIPRIREASARKEAVSLAYQGNVVDLWERLAREDL
                     RVDLGSDQTSLHNPFAGGYYPAGLSFEESNRMMVDNPELFRQKVYESLRRQVKAISAL
                     TERGMYFFDYGNAFLLESSRAGADVLKPNGEFKYPSYVQDIMGPLYFDYGFGPFRWVC
                     TSSNPSDLAETDAIALEVLENIASHAPEEIQQQLHDNIHWIREAGRNNLVVGSQARIL
                     YADSEGRLRIAEAFNKAIREGRISAPVVLGRDHHDVSGTDSPYRETSNIYDGSRYTAD
                     MAIQNVIGDSFRGATWVSIHNGGGVGWGEAINGGFGLTIDGSADAERRLKMMLHWDVN
                     NGIARRSWARNHGAISSIKREMERTPLLKVTLPNMADDNLIDDVVKG"
     gene            18557..19159
                     /locus_tag="PK502_02630"
     CDS             18557..19159
                     /locus_tag="PK502_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPG99746.1"
                     /translation="MKSKTWITIGIVVLVAVILYISIKGSYNKMVQKDEAVTSQWANV
                     ENVYQRRLDLIPNLVNTVKGYANFEQQTLTQVVEARAHATQVKVSPENLNQESIQQFQ
                     AAQNELSGALSRLMVVVEQYPELKANQSFLDLQAQLEGTENRIAVERNKFNEVVRDYN
                     TYIRRFPSNIWAALFNFDKRGYFESEAGAEKAPKVDFGTN"
     gene            19226..19660
                     /locus_tag="PK502_02635"
     CDS             19226..19660
                     /locus_tag="PK502_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPG99747.1"
                     /translation="MKAKDYFTLEEQKRITDAVEQAEKNTSGEIRVHVENECRIDVLD
                     RAVQIFAMLKMHKTKLRNGVLIYLSLKDHKFAILGDAGINAKVPEDFWDSTKQAMLEE
                     FQENRLTEGIIKGITMAGEKLKEHFPYKQDDVDELTNDISFG"
     gene            19690..20475
                     /locus_tag="PK502_02640"
     CDS             19690..20475
                     /locus_tag="PK502_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPG99748.1"
                     /translation="MITKQLRNILLAAALLLPNISWSQDIPDPMNPPRLVNDFAGILG
                     GEERDALESMLRGYHDSTSTQIYVVTVNDLSGYDISDYAFRLGEKWGVGQKSKNNGVV
                     ILIKPKVGNQKGQAFIATGYGMEAIIPDAVARRIVDNEMIPYFKTNSYYKGIVAGCKV
                     IIDLAGGEYTADAYYKKESPIPGIILLIIFFGFIFLSGGRRSKLRSGSIGKNIPFWIL
                     LGSMGSGRRGGGFGGFSSGSGGFGGFSGGGGGSFGGGGAGGSW"
     gene            complement(20799..21170)
                     /locus_tag="PK502_02645"
     CDS             complement(20799..21170)
                     /locus_tag="PK502_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HPG99749.1"
                     /translation="MEENLITEKIIGCAIEVHKQLGAGLLESAYEECLFYELINKGLN
                     VKKQLALPLVYKEIKLDAGYRIDLLVENKVIIEIKSVDAIADIHKAQLMTYMKLANIK
                     LGLLINFNVLRLKDGIVRWII"
     gene            complement(21645..22100)
                     /locus_tag="PK502_02650"
     CDS             complement(21645..22100)
                     /locus_tag="PK502_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HPG99750.1"
                     /translation="MAYREVSFRISRKNFWFITFLIVLLPISSKYRLLIFGKRTQGVV
                     VANEVKSSAFYQSHGYDSYPVIEFNADSLTLRMYGPENTNYEVGRRFTVIYNPDNPYN
                     CMILSLSYFYTGRGVILPGTIMLLWISFYLTFRSRDSKNNPRKGERDSP"
     gene            complement(22104..22691)
                     /locus_tag="PK502_02655"
     CDS             complement(22104..22691)
                     /locus_tag="PK502_02655"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPG99751.1"
                     /translation="MPFIENSVSNKRNGWIEVIAGSMFSGKTEELIRRLKRAKYARLR
                     VEIFKPQIDTRYSEEKVVSHDANSIISTPVTSSGNILLLAQDVDVVGIDEAQFFDMGL
                     PRVCTQLADMGVRVIVAGLDMDFRGKPFGPMPNLMAIAEYVTKVHAICVRCGELAHFS
                     HRTADTDKLVVLGETDIYEPLCRHCYNEALSKSKT"
     gene            22782..23522
                     /locus_tag="PK502_02660"
     CDS             22782..23522
                     /locus_tag="PK502_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99752.1"
                     /translation="MQYLRDSKTLLDDSMKIIERALASENLPSIERNILLDKLAKAYE
                     LLLFSVEKQAEPTHQAHSQHTVKANAETSTQGIKRDQPLSTPSHNVEPVAPAGSYSKK
                     PPEQIQKFREKAPSTNESKSIEVEPTPGAASTDRPATLADKFQDKQKSLNETIANQAR
                     RSDMGTKLQNKPITDLAKAIGINDKFLFTNELFDGDSALYAETVNRLNEMDDLNEAII
                     YLQDNFSWSATSEAASKFIDLLRRKFAL"
     gene            23668..24363
                     /gene="rsmI"
                     /locus_tag="PK502_02665"
     CDS             23668..24363
                     /gene="rsmI"
                     /locus_tag="PK502_02665"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPG99753.1"
                     /translation="MSKLFIVPTPIGNLGDITLRALEVLRSVSLVLAEDTRTSGNLLK
                     HYSIETPLRSHHKFNEHQSVEAIAEQIAAGLDVALISDAGTPGISDPGFLLVRTCLER
                     GVEVECLPGATAFVPALVQSGMPCDRFVFEGFLPQKKGRSKRIEALQDEDRTMVFYES
                     PFRLVKLLEQLMAVFGPDRRVSVSRELTKLHEETMRGTLAEVAAHFNSKTVKGEVVVV
                     VQGLGRNKSLDEE"
     gene            complement(24420..25178)
                     /locus_tag="PK502_02670"
     CDS             complement(24420..25178)
                     /locus_tag="PK502_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPG99754.1"
                     /translation="MKNKPAEPTAELSVEEKLRFLYMLQQVDSKIDRIRMLRGELPLE
                     VQDLEDEIEGLETRIDNLKQEIKSLESEVSAKKQEIKDANTLIKKYQEQQKTVRNNRE
                     FDSLSKEIEYQTLEIELCEKRIKEFTQQVKDKTLVIEQSQKLLEERKLDLKGKRMELD
                     NIINETQKEEEELIRKSEEYGKIIEPRLYTAYRRIRGNARNGLAVVTVQRDACGGCFN
                     KIPPQRQLDIKIHKKIIVCEYCGRILVDDSLASM"
     gene            complement(25198..>25475)
                     /locus_tag="PK502_02675"
     CDS             complement(25198..>25475)
                     /locus_tag="PK502_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPG99755.1"
                     /translation="SRQVIKRVAFCGGSGASLIGKAIGSGADIFITSDIKYHQFFDAD
                     GRIIIADIGHFESEQFTIELFYEYLLKKFPTFAVLKSRVNTNPINYL"
BASE COUNT         7131 a         6053 c         5678 g         6613 t
ORIGIN      
        1 atcatcccct cggagctaat accacgtagc ttcgcctttt taatggtaac ctcacctccg
       61 ctaaagtaaa gcgtcgtgcc aatggttgcc accagtacct tctgaccttt tgccacatta
      121 ggtgccccgc aaactatgca aaggggcttt tgttctccaa catcaactgt tgtaacgcta
      181 agcttgtcgg catcgggatg cttatcgcaa gtcagcacct cccccaccac tactccggca
      241 aggccacccc taatagcttc tatgcgctcc atgctatcta cctccaatcc agtagaggtg
      301 attacttcag aaacacgctc cggtgccaga tcagtatcaa cgtactgttt aagccagctg
      361 taggaaattt tcactgtgat ataattagtt aagcacttga atttatgtga tatacattca
      421 gaattaaatc aacctcctta actacggagg catcagtacc ataccatttt tctgttagca
      481 aaatgggttg tcggcatttg tcagcaaaaa aatggctaca ccgacccacc aataacgttg
      541 caaattttac caaggaagca tttgccggca aaagcattat aaaaaatccg ccatatcctg
      601 ctaacccttt gtggtagcaa aagtaagcat tattgaaaaa ctccccaaaa tgccatccca
      661 ttcatcagaa aaaaaatcta cagggaaaac tttattagaa attatataca tttacggcgg
      721 aaaatgaaac cgaattttcc acgtcaccat gaataaaacg acccgcaata gcataaaaat
      781 gaacatgatg ctccataaag gcatgatgcg taaatctatg agcataaaac gagtgccgtt
      841 gcaggtctta ctctcgcatg taaagtacac tttgtggtaa tatactaaaa actaagcgat
      901 taaaaggcct gcattgtttg caggcctttt tttatttttt aaatcctaaa agcacagaaa
      961 tgaaaatact caaatttggt ggaacatcgg ttggggatgc ccaacgaata aggaaagtag
     1021 cgcagctgct acctgaagca gaaccctgca tcgtggtgct atcggccatg gcgggaacca
     1081 cgaatgcctt ggttgagata gctaaccata gtagtagtgg aaatgttagc atggcgatag
     1141 aacaggccaa acagctcgaa gcaagataca tggaaacatc tcacgagctt ttcaattcgc
     1201 cggttttcag gaatagagga gaaaagttta ctaatgatat gtatgccgag ctttacagaa
     1261 agcttggttg tccgcctaca aaaaaagtta cgcttgaaat agttgcccta ggcgagcagc
     1321 tttccacggg tttgttccac ctctacctca cagaaaacag aatccgctca acctaccttc
     1381 ctgctttaga gtttatgagg attgacaggg agagagaacc cgactacttc tacatttccc
     1441 agagcctgga acgattaatc aaagccaatt cctttacccg ggtattcatt accgaaggat
     1501 ttatctgtag gaatgccaag ggccaaaccg ataacctggg tagaggtgga agcgactata
     1561 ccgccgcaat aataggaaat gtagctgatt cggaagaggt gcagatttgg acagacattg
     1621 atgggcttcg caacaatgac ccccgccacg tggttaacac caaaccaatt aggcacctat
     1681 cgtacggaga agctgccgag ctggcctact tcggtgcgaa aatactacac ccgtcaacca
     1741 tccaaccctg cagcgaaaag aatatccccg tggtgctgaa gaataccctt cagcccgacg
     1801 accccggaac ccttatcgac tcggtctata taccaaaagg aattaaggcc atagctgcta
     1861 aaaaaggcat aaccgccatt accatccaat cgtcgagaat gctgctggca tacgggtttc
     1921 ttagccatgt attcgaggtg ttcaaccgtt acaaaactcc tgttgacatg attgccacct
     1981 ctgaggtttc tatctcgcta accattgacg attgctccaa cctacagggt attgctaacg
     2041 agctggcaga attttcaacg gttgaatgta cctatgacca aactatagta tgcattgttg
     2101 gcgattttgg cgccaaccgc aagggctatg cagcccgaat cttcaatgca ctgcacgaaa
     2161 ttcccatcag gattatatca tacggaggaa gtagcaacag catttcgatt ctggttgatt
     2221 cccaatcaat gaccagaacc ctgcagctgc tgcaggaggt tgttgataca gatgtagatg
     2281 ttaaccttga acagacggcg aatacaattt catactagca caccatgaag acaccttact
     2341 accagtacga cctgcagctt ttaagacaaa cactcgatgc agcaacaaga gaagctaaca
     2401 aatatggcta caccctacac tatgcggtga aggcaaatgc caatccggta atactcaacg
     2461 aaatatcatc gtacggactc ggtgccgatt gcgtgagcgg taatgagatt tccgcggcac
     2521 tatcgggtgg gtttaaccct aaaggcattg tgtttgccgg tgtaggtaaa accgatgagg
     2581 aaattgagct ggccatagcc aggggcatat actgcataca ctgcgaatcg gttcaggagc
     2641 taatgaccat tcaaaaaatt gcaagcgcca tgcaaacaac cgccaacgtg gcccttaggg
     2701 taaatcccaa cgttgatgcc cacacccaca agcatatcac taccggaacc atggccaata
     2761 agtttggact tacggtggaa gaggccaggc gaattataaa aaattctgac cgctacagca
     2821 accttaacct ctttgggctt cactttcata ttggttcgca aatcaccgaa atgaacgtgt
     2881 ttgaggagtt ggcacaaaga gtaaatgagc ttcaacacga cttctacgcc aatggtaagc
     2941 ctgcctacct caacctgggc ggagggttgg gaataaacta ccagaatcct gattcagagc
     3001 ttatccctcc atttatgaat tactttgatg tttttgcacg gaaccttaac aggaactcct
     3061 ggcaaagcat tcacttcgag ctggggcgct cgttggtggg ccagtgcggc aagcttgtaa
     3121 ccagggttct ctacacaaaa aaaatgggta gtaagaattt tgccattgtg gatgctggaa
     3181 tgaacgccct tatccgccct gcgctttacg gcgcctacca cagaatagtg aacaccagca
     3241 ccagcagctc caaaatggaa cgctacgagg tggttggccc gttgtgtgag tcaaccgata
     3301 ttcttggaac taacgttttg atgcctgtaa cccagcgaaa cgacgtgctg gaggtacttt
     3361 cgtgcggcgc ctatgccgag gccatgagct cacgaatcaa ccttagggat attcccgatt
     3421 actgctttac caaccagctg caggaaaaaa caattacaaa aacaggagct cctctgcagg
     3481 gctgatatca actcaattag ctacagttgt tgcctatatt ctcaaaaccc atgttagtag
     3541 ctattctata aggtgaatgt tgctgtatgt tcagtagaat gaaccgatgt ggcatgccac
     3601 tgcgtaagga cttgatttgg tggttaatat ttttgagaaa attccaagaa atttacctgc
     3661 tattatcgtc gactaactaa ataggttagc gccatgagaa agccattgat attacttttt
     3721 gtggttgtta ccttgagttt ggcatcatgc tacaaaaaca acgctacatt taccgttcag
     3781 gtaaacgact atgcgccaac ctacacggtt gcttacacgg ctcaaaatgg ggtatgattg
     3841 ggcagcctta aagttccgaa aaactatgag tttttcaacg agtactccta tggctggcag
     3901 ctaaacctca aggttaaggc ggaagacagc atatttgtat atgtgcaaga acgagggaac
     3961 ctgaaagaac caattataag tgtaaggatt cactagaagt tcataaaatc cataccattc
     4021 tgaaccaatt aattcatgtg aaattggatt tgaattgcat cattttctta gaaaggttta
     4081 attgatccat gtagctgttt accagtattg tagcaaaata tgagtttgca atttaagggg
     4141 tggctatata tttcaaagat ttcttttact tttactccgt aaaacgcagc tatatgtttc
     4201 aggttgatag aataatactc agcagtaagc acacaggatt tgaaaattca acatgctttt
     4261 gtaatgaatg tctgcccatt ccggcagact ttttttttac ccaacagttg gcggtagctg
     4321 cagctgcaaa aaccacatcg caaaataact aaacagctaa aactatctat gaataagcaa
     4381 aaagtaaagc tggtgctggc agatgggagc gagtattccg gctactcctt tgggtatttc
     4441 ggcagcactg caggtgaggc cgtattcaac accgccatga acggttatcc cgaaagtctt
     4501 accgatccct cctaccgtgg acaagtacta attgccacct tcccgttggt tggaaactac
     4561 ggcgttccgg aaggaataac cgagggaaat ctacataggt tctgggaatc agatagagtt
     4621 caaatatctg ggctggttgt atcggtatat aatgaggatt tcagccactg gaatgccgcc
     4681 aaaagtcttg gcgattggct tgctgaatac cgaattcctg caattgccgg catcgacaca
     4741 agagctttaa cccgtaaaat acgggagaag ggtagcatgc tggctaaaat attggcttta
     4801 aacgatgtgg aatggcacga cccaaactcc gaaaacctgg tggcccaagt cacggtagct
     4861 caaaaagaaa tctatggaaa tggggagtac agggtagttc tggtggattg cggggtaaaa
     4921 aacaacatta tccgatgttt aacgcaacgc aacacaacgg tggtaagggt tccctacgat
     4981 tacgattaca ccgatgaaga ttacgatggc ctgttcatat ccaacggacc gggaaatcca
     5041 aaaatgaaca ctgccacaat tgcggttctg caaaaggcaa tgaaaagtaa taggccaatt
     5101 atgggtatat gcctggggaa tcagctgctg gctttggcat ccggcgctga cacctataaa
     5161 cttccctttg gacacagaag ccacaaccaa cctgtaattc ttaagggaac aaacaggtgc
     5221 ttcattacct cgcaaaatca tggctttgca attgactcct caaccctttc ggacgactgg
     5281 gacgtgcttt ttgagaatgc caacgacgga acttgtgagg gcatcaggca caaatctaaa
     5341 ccaatatttg cagtgcagtt tcatcccgaa gcgtcgggag gcccaaacga tactgaattc
     5401 ctatttgacg aatttcttca gttgatggta aagcataaac aaatcaacaa gtaaccgtat
     5461 gaacagcaaa tatcataaag tactggtgct tggctcggga gcccttaaaa tcggcgaagc
     5521 cggagagttt gactacagcg gatcgcaggc actaaaagcc ctgagggagg agggggttaa
     5581 aagcatactg ataaatccta atattgccac cgttcaaacc agctggggag ttgccgatag
     5641 ggtttacttt ctaccggtaa ctccgtactt tgtagagcgg gtggttgaaa aggagcgccc
     5701 tgacggaata tttttatcgt ttggaggtca aacagcattg aactgtggaa ttgagctctt
     5761 caaaacagga tttcttgcta agcacaacgt tcaggtgcta ggaacaccag ttgaaaccat
     5821 catcaacacg gaagacaggg agctgtttgc cagacaactc tcacaaatta atgttagcac
     5881 gccccgcagc caatcagcaa gatgccttga tgatgcacta caggccgccg aaaaaattgg
     5941 ttttccgctt atagttcggt cggcattcac attgggaggt cagggaagcg gtttctgctc
     6001 aaacgctgaa gaactccggg agctttccat taatgccttt tcatactccc ctcagattct
     6061 tgttgaggaa tcgctatggg gatggaagga ggtggagtat gaggtagttc gcgaccgctt
     6121 cgacaactgc atcaccgttt gcaacatgga gaacctcgac cccctgggta tccatactgg
     6181 tgaaagtatt gtagttgcac cctcgcaaac ccttaccaac gctgaatacc acaagttacg
     6241 atcactttcc attaaaatca taagacatct gggcattatt ggggagtgca acgttcagtt
     6301 tgccctcgat ccgaaaagtg aggactaccg tgtaattgag gtaaacgccc gtctgtcgcg
     6361 ctcgtcagca cttgcctcaa aggcaacagg ctacccactt gcctttattg ctgcaaaact
     6421 cgggttgggc tacggccttc atcagcttaa aaaccctgta acagccatta ccactgcctg
     6481 ttttgagcct gcaatggatt acatcgtcgt aaaaattccc cgatgggatt tgaataagtt
     6541 ttcgggcgtc agccacgaaa taggctcatc catgaaaagt gtaggagaga taatggccat
     6601 tggccgctcc tttgaggagg ccattcagaa gggtctcagg atggtcggac agggcatgca
     6661 cgggctggtg gccaacacaa agctcaactt tcccaacctg gaagaggccc tggtccaccc
     6721 taccgatatg cgaatttttg ccattgccga agcattggag ctggggtgga cggttgaaaa
     6781 agttcacgag ctgacaaata ttgacaggtg gttcctcgga agaatgcagg gaatcattac
     6841 ccttgagaaa aagctgatgc aggttgcatc agttgaaatg ctggataggg aaaccctgct
     6901 tgaggtgaag cagtttggct tttccgactt tcagctggcc aggctgatat acaatcctgg
     6961 tccagatgat atcgaaaccc gccagcaagt ggtacgatac cacaggatac aacttggtat
     7021 tgttcctgtg gccaagcaaa tagacacgct tgcagccgaa tatccttccg aaacggatta
     7081 cctctacctg acctatcatg gtattgaaaa cgacacatca cctaaccaaa gcaaccgaac
     7141 catagttgta cttggttcgg gagcctaccg gattggcagc tctgttgagt ttgactggtg
     7201 cacggtaagt gcagccggca aaataaggaa gcttggctac agagcagtaa tgatcaactt
     7261 caatcccgaa acggttagca ccgactacga catgtgcgac aagctgtact ttgatgagct
     7321 aacgtttgaa cgggttatgg acatcattga gttagaaaaa cctattggtg taatggtctc
     7381 catgggaggc caaatcccca ataacctggc catgaggctt cataatgcag gagtaaaagt
     7441 attgggaact tcgcccgaat ccatagatag ggctgaagat cggcaaaagt tctccgaaat
     7501 gctcgacatg ctgcacatcg accagccccg atggaagcag ctatccaccc tcgaggaggt
     7561 tgaacgtttt gttgacgagg tgggcttccc ggttctcata cgtccatcct acgtgctgtc
     7621 gggctcggcg atgaatgtag tttccaacgc cgatgagctt cgtcactacc tcgttctggc
     7681 ggctaatgta tccaaaaccc acccggttgt cgtaagcgaa ttcatcgagg gtgccaagga
     7741 aatagaattc gatgccgtgg ccaacaacgg agaggtgatg ttcgacgcca tttcggagca
     7801 tattgaattt gcaggtgtgc actccggtga cgcaaccctg gtttttccac cgcagaagct
     7861 atactttgaa accatccgtc gaatccgaaa aattgccagg gcaatagcta aggagctcat
     7921 gattacaggc ccattcaaca tgcagctgct tgcactgaac aacgaggtca aggtgattga
     7981 gtgcaacctg agagcaagca ggagcttccc atttgtctca aaagttttgg gcttcaactt
     8041 tgttgaggct gcaacggagt acatgctggg tcggacaccc ggctacctac ctccttccat
     8101 gtttgaactt gaccatgtgg gcataaaagc gtcgcagttc tcatttagcc gtctttcaaa
     8161 ggccgatccg gtactgggcg ttgagatggc ctcaaccgga gaggttggct gtttgggaac
     8221 cgattttgac gatgcccttc tgaaatcgat gctttcggtg ggctaccgag taccgcaaaa
     8281 atccattctc gtttcgtcgg gaccattacg gtcgaagctg gccttagtag aacctttacg
     8341 actaattgag cgttgcggca ttactatcta tgccactcgt ggcactgcca aattccttgc
     8401 cgaaaatgac gtaaacacta ttccggtggc atggcccgat gaggacagcc agcctagcag
     8461 tatcgagcta attggtagta aacaggttga gctggtgatc aacataccta aaaacctatc
     8521 acgagaggag ctgaacaacg attacgacat aaggcgcgca acggttgact acaacattcc
     8581 gcttattacc aacgcccggt tggcagcagc attcctaatg gccatctgca ataaaaacat
     8641 aaacactctc gacctaagaa gctgggatga attttttgaa accaaacaat aataaatgca
     8701 tgtatttctg aacgttaatt atattttcct gcgtttaagc accgactagt aggttcgggt
     8761 catgctttcg ggaaccacta tgatgaaatc ggcaatgccc aaatttgcag catccaactt
     8821 gtcaacatcg gtctgcatca cagtagccac ggttaccacc cttaggttgg ggttggtccg
     8881 gttgatatac cagactatac cttccttgtt atcggaatga taggaaccat tgtagtggat
     8941 aaaggtggaa ccttctttaa agttggccat tatgaaatga gccatggtgg catcctttaa
     9001 tgcctgcgat ttgggcaagt tctgagaggc ctgcatgcca gctccgtgca tgttgtccat
     9061 acccatcata gccttatagc ccggaagctc aggatcgaag gcaaccggta agggtgcaaa
     9121 aaattttcgg gcttcatcag gaaaactatt cagcacctca aagcctcctc cagccactgc
     9181 agaggcgtac ctgcggggaa tattggtggc aacgaaccta accccacttt ccttggcaaa
     9241 ctcaaccaac ggtttatagt cggtctggta gttgttccaa agttttgcct catcctcaaa
     9301 gcgtttcatg gagataaggc cagacatgta ctcatcgagt atgagctggt tatcggcctc
     9361 aaacatctcg gctcccataa tcaggctgcc gtttttcgcc ctaaaaaggt cttttgccag
     9421 ctccagctcc agccagtggg ctatggggtt atcgtggagc tctccaaaaa acacaacatc
     9481 ggtagctgcc gatctattaa ctaaatcaga gtacgaagcc acatttccct ttgaatcgaa
     9541 aatggtgtag gcatttttat cctgggcaaa ggaaaaccct gctgttaaac aaacgattat
     9601 gcaaaaaatg acttttctca tattttttta tatttgccaa acctgggatt tacaccttta
     9661 gctaaacaac gagctaaagt aaacattttt aacaattttt tctaagcatg gcgatatgga
     9721 aatcacacga acattcgaca ttctggaagc cggaaaggaa acctatgaga atcgtccagt
     9781 tctggcatcc aggcaaaacg gaacctggga ggagtactcc atagataagt actcacaaat
     9841 tgcgcacaac ctgagctacg gattgatggc cctaggtcta agacctggcg atagggtggc
     9901 caccgtgtcg ggtaaccgac ccgaatggaa cttcgttgac atggcccttt cgcaggcagg
     9961 catggtgcat gtacccatct accccaccat cagccttgat gagtataggt acatccttaa
    10021 gcattcgggg gtaaaaatga tttttgcagg cgataagagt atttacggca agcttcaacc
    10081 cctcaccggt agccttgaga attttatcgg catgtactgc tttcagaata ataacgaact
    10141 tcccagctgg aaggaaattg ctgaaaaagg gaaaagtgtt gccaacagct atattgatga
    10201 gcttttcaga ataaaaaaat caattagtgc cgatgatgtt gtttcactaa tctacacttc
    10261 gggaacaacg ggcatatcaa aaggtgtaat gcttacccat aataattttc tcagcaatgt
    10321 aagggcttgc accggacttt ttccgttaga accaggagat aaggccgtta gctttcttcc
    10381 acttagccac gtatttgagc gtatggtgaa ctactactac caaagcattg gccttacagt
    10441 ttactacatc gacaacctgg ccaacctggg gaatgtcatc agcgaggtaa agccacagat
    10501 attcatgacc gtaccgcgaa tgcttgagca aacatacaat aaaattatag cggtaggaaa
    10561 ggatctttcc ggtctgaaga aacaggtttt cttctgggcg gttaatctgg ggtaccacta
    10621 caacgagaat ggcaatagcc agtactacaa catgaagttg agactggccc gtaagctggt
    10681 ttttgagaaa tggcaggaag cgatgggagg aaagataaag ctgattgtaa gcggcggtgc
    10741 accgctccaa ccccgtttga tgagaatttt ctgggcagcc ggcatgccgg tttacgaggg
    10801 ctacgggcta acggaaacct caccagtgat tgccgtcaac cacagcgcta cccccgggaa
    10861 tgttcgattt gggtcggttg gccctgtcct ttctaatgtt gaggtgaaaa tatccaacga
    10921 tggagaaata ctcatgcgcg gccccagcct gatgaagggc tactataacg ctcctgagct
    10981 tacaagccaa gcaattgact ccgatggatg gtttcatacc ggtgacattg gacaccttga
    11041 tgagggaaaa ttcctaacca ttaccgacag gaaaaaagaa atctttaagc tttccaacgg
    11101 caagtatgtt gcccctcagc tgattgaaaa caagctgaaa gaatcatttt tcattgagca
    11161 gtgcatggta gttggtgaaa acgagaagtt tgtcagcgtt ctgatttcac ccaacttcag
    11221 ctttctccac gattggtgca gccaccacaa gatacacttc cagaacaacc aggaacttat
    11281 caccctaccc gaggtgattt cccgatttca aaaagaaata aatggcataa acaagcagct
    11341 gagcgactac gagcagatca aacgctttag gctggttccg gagcaatggt cgcaagaaac
    11401 aggcgaactt tcaccaacgc tcaaactccg tcggaagttg ctgtatgacc ggtatgaaaa
    11461 actattggag gacatttacg ctcatccaaa gttggttgaa attaggggaa caaatggcga
    11521 ctaattactc ggaaattaaa tagttgaccc tggggtttta cctctgccac gggtttaacg
    11581 ccagccctcc ccaaataatc gctcaaggga atcggatcgt gatgtttacc ccagctgtag
    11641 gttgtttcat actgctcctg cagcttgtta aagttaaaca tacagtaggc atgctcaaag
    11701 tcaggtacaa atacgctggt tttactagcc aactccggag cctccttttg cattgcagcc
    11761 aaccggttga agaaacttct gtttgttatc gcatcgggcg atatgttgaa cctttccacc
    11821 agacctaacc tttcaacgcc cagctgcttg agttcttcag caactgaaag ggtatcgcgc
    11881 ttacgactca cgataatcac tttttcgacg ggcacacccc gaaatttctc atactcaact
    11941 agtgcttggt atggcacatg gtcggcggca attccgccgt cgtagtaggg aacattaggt
    12001 atggttctta catttttaat gggggacggt ggaaaaatta ccgggattga ggttgatgcc
    12061 atcagaacat caactaagtt gagcgaagga tcgctttcag ggttaatttt taggttgcaa
    12121 agccggtatg ttctatcgct gaaaggggct attttgaagt taaccaccga aatggaagtg
    12181 ggaatcggca aatcggaaag aattcggtag cctaacctat cgcacacaat gctgctaata
    12241 gtatttctta gcggggttgt atttacgggt aacctgttcc catccggttt aaaaaccttc
    12301 tcgttcgata gctgacccag aatgctcttg taatccttcc acgttaactt accctcaaga
    12361 attgcgttca gcataacagc attaagcgcc ccggagctgg cgccagaaat aaataccacg
    12421 tttttaagca ttcccttttc gtagagattc tgaagaaggg ctgcctcctg ggctattttt
    12481 gcagcagctc ctgttattat aattgcagtg ccgttgggct tttgaaggct ggttggcctg
    12541 gcttgggcaa ctgctacacc tggcaatacc caaaaaaaca tcagtgctac aatgcaatac
    12601 ctttttactg aaaagaaagc gggttttaac cagcacagcc ataaattcgt cataatcttc
    12661 gtatgaaatt agctcatgtc caaacgctgc tatatagctt tggttgtctg gcttaaggct
    12721 taaggatact tgccgtcatt ttacttcaaa ataggcttag tccaatgtta aagcatcccc
    12781 ccacaacggc tgctgccaca gcaaagagtc aaatcatgct atcagtatca ttaaagtccc
    12841 cagcataaaa aataccaccc caacagcaat ggaaagaata tcctcgttaa ccctattggc
    12901 aaacctggcg ctgactacgc cgctcaaggc agctcccgac cccatcacaa gtccaggaac
    12961 aagccttata ttgccatgaa taaagtaacc aagtgctccc gagaacgaag ttatagccat
    13021 aagaagcgac gatgttccta tagctaccct tatgggaaag ttaagcacaa aaactaaaat
    13081 caaaagaatc attcctcctc ccccggcacc gaaaactcca gaaataatgc ctatggcaaa
    13141 acccagcata agaattgcca tattcttacg cttctccgtt ttgaactcca aaaccggttt
    13201 ggtactttcc agctttttcc ctctaaaacc cttaatgcaa atcaggatgc ccattagaat
    13261 aatgaatatt ccaaaagagc cccccagtcc ggtctccgga atttgatcgg caaaaaatgc
    13321 tccgatttga gccccaaaaa ttgaacccaa cgcaatccaa acgcctaccc ttacatcaat
    13381 attgccgttt ttaaagtagg tataagaaat agctatgggg gctataacgt tgactatcag
    13441 gctaattcct atagattcat gaataggaat acccagcagc atgttaacca gaggaacaac
    13501 aatcataacc cccgacgatc cggtgagacc tgttactact ccggttacga gtccaatgat
    13561 tattaaagct accagtgtag ttgacgacat tacaacatta tgtaattata gtacgtatcc
    13621 agaggctaag ttaaagcaga aagccgagta agcaaaaatc aacatagctt caacgtaatt
    13681 gacaaaccag cgctccttaa cttcaaaaat accatcacca acagcagctg ttcatttatt
    13741 ttatattgaa attgtaaagt ctgtaaaagc taatcgaagt agccgcgaat acggtcaaat
    13801 atgggagacg actcctttgg attgggtgta aaattggatg acttggcgag tttttcgaaa
    13861 agggctcgct cctcacgggt caaggtcttt gggatgtaaa cgttaatcgc cacaagtaaa
    13921 tcgccacggc catagctgtt tacctcggga agtcctttac ccttcaacct aaggatttta
    13981 ccaggttgag ttccgggttc aatcttaatt ttgaccttcc catccaccgt tggaatttcg
    14041 gcaggagcgc ccagaaccgc atcgggaatg ctgatggtaa ggttgtagat caggtcgttg
    14101 ccatcgcgga ttagctgtgg gtgtttctcc tcctcaataa ccacgagcaa gtcgccgggc
    14161 acgccacctc gtcgggcagc gttaccctta cctgacacgc tgagctgcat accctcggct
    14221 acaccagcgg gaattttaat ggtaataatc tcgttatccc tcaccacgcc ttcgccatta
    14281 cactccgtgc attttttagt gatggtttta ccctcaccgt tgcaggtggg acatggtgcg
    14341 gtgctttgca tctgccccaa cagtgtgttg gtaactcgtg taacatatcc tgatcccctg
    14401 caggtggagc atgtctgaaa gtccgatgga ctggcagctc cgctaccatt gcacgaatga
    14461 caggtaacgt acttgtttac ctttaacttt ttctctacac cattggctat ctcgctgtag
    14521 ttgagcttaa ccttaacccg tatatcgctt ccacgcgagg ttgccctctg gttagcccta
    14581 ctgttgccaa atccaccaaa tccgccaaaa ctgccaaaat gaccgccaaa aatatcacca
    14641 aactgtgaaa aaatatcatc aatggtccag tcatgggtga aaccgccacc tccaccacca
    14701 ttgcctactc cggaatgccc aaactggtcg tagcgagcac gcttggtgtc atcgcctagc
    14761 acttcgtagg cctcggccgc ctccttaaac ttttcctcgg cttccttatc acccggattc
    14821 ttgtcggggt gatacttcag cgccatttgc cggtaggcct ttttaatttc atcctttgtt
    14881 gcattccggc taacgccaag aatttcgtaa aaatctcttt ttgtcgtcat gctctaccca
    14941 ctcctattcg ccaacaacaa cttttgaaaa acgcatcacc ttgtcgtgaa gcatatatcc
    15001 cttctgaata acatctacta ctctcccctt ctgtgattca tcctgaacgg gagttttggc
    15061 aaccgcctcg tgcaaatcgg tgttcagctc cgagcccatt gcttcaattt ccttaatgcc
    15121 cttagaattg agaaaatcgt taaattttga aactatcagt gataggcctt ccttgactgc
    15181 agtaatctca tcggttgact caatagcttg aagggctctc tcaaggtcat caaccaccgg
    15241 aagcaaatcc ttcagtacaa cctcggcaga gtacttgagc atatccgatt tctccttcag
    15301 cgttctttta cggtagttgt caaactcggc cgaaagtcgc aggtgcttgt ccttaagctc
    15361 ctccaactcc ttctgtaact tctcaactaa ggctttatcg gttgcatcag cctcttccat
    15421 tacgttttcc gaagcaggtg ctgcattggg ctcagcccct tcggcggaaa ttttatcctc
    15481 agcctcagga atatctacct gctgctgggt ttccagctcc ttttcatcct cagcagcttt
    15541 gctttgcttg ctactcttac tcatatctca attatatttg tttcattttt agatggttac
    15601 acgataaacc gcttacctac aaataatgaa gtaatacttc ataggggaaa atccttgtta
    15661 tacacaattt aaataccaag cagaaacgct cccccaccgc cattctgtcg cccagtccaa
    15721 tcaaatatta ttccagcagt gcaatggcag acaaaatgac acaaaagtag taaaaaatag
    15781 gggatgttta taccgatagc caacgcttaa ttgttcggct tttatcagaa taaagaaatt
    15841 ttagcagtta aactactgct tgttacatca gtattctcga gcacagatat tattatgggg
    15901 acaaaaaaga ataagtgata aacccctgtt ctagggtttt tcaaaatgtg ccatcacgaa
    15961 gggatcgttg tcaatgcccg aaacgctgta ctcccagtca atggtaaagg aaaggggatg
    16021 aaagtacccc tttcctgaaa tgatgtagga atcgctgttg cagcccagta tgttgaaaat
    16081 cgtatcgtta agctgaacat aggtctccac ctcaaggccc aggttatata ggttataaat
    16141 tatgtaggag gttgagtcga gctggtcgta gtgctccatg ctaacgttgt aggttctgta
    16201 gctatttccg gggtatattt ccctactccg ccacacgccg gtaatggtgt ttgtgctatc
    16261 gagctcgtcg cagctactgc cacataggac caagatggca atcaacacgc ctgtttcccg
    16321 aataaatttc tgcatagcca ttgcatcaag gtgaaagggt gaaggggcgc ttaccccttc
    16381 accacgtcat caataaggtt gtcatcagcc atgttgggaa gtgttacctt taacaggggt
    16441 gtgcgttcca tttcccgttt tatggatgaa attgcaccgt ggtttcgagc ccagcttcta
    16501 cgggcaattc cgttgttcac atcccagtgg agcatcattt taaggcgtcg ttcggcatcg
    16561 gctgaaccat caatggtaag cccaaacccc ccgttaatgg cctctcccca tccaacacct
    16621 ccgccgttat ggatggaaac ccacgttgcc ccgcggaatg aatcgccaat cacattctga
    16681 atggccatat cggcagtgta gcgtgatcca tcgtaaatgt tggaggtttc gcggtagggg
    16741 gaatcggttc ccgaaacatc gtggtggtcg cgcccgagca caaccggggc ggaaattctg
    16801 ccctcccgga tagccttatt gaaggcttca gcaattctaa gcctgccctc cgaatcggcg
    16861 taaagtattc tggcctgcga gccaacaacc aggttgttcc taccagcttc cctaatccag
    16921 tgtatgttgt cgtggagttg ctgttgaatc tcctctggag catgacttgc aatattctcc
    16981 agaacctcca gggctatagc atcggtttcg gcaaggtcgg aagggttgga agaggtgcaa
    17041 acccatcgaa atgggccaaa gccatagtcg aagtacagcg gacccattat gtcctgaaca
    17101 taggagggat atttaaactc gccattaggt ttcagcacgt cggcaccagc cctgctactc
    17161 tcaagcagga aggcgttgcc gtaatcgaag aagtacattc cacgttccgt cagggctgaa
    17221 atggccttca cctggcggcg aaggctttcg taaaccttct gcctaaacag ctcaggattg
    17281 tcaaccatca tccggttcga ttcctcaaag gatagccccg caggatagta gcctccggca
    17341 aatgggttat gaagcgaggt ttggtcgcta cccaggtcaa cccgtaggtc ttctctggca
    17401 agcctttccc acaggtcaac cacatttccc tggtaggcca acgacactgc ttccttccgt
    17461 gccgaggcct cgcgaattcg tggaattagc ttttccaaat cggtgtaaac ctcgttcacc
    17521 catccctgtt ggtaccgttt ttccactgcc tttgggttga cctcggcaat cacccccacc
    17581 acgccggcta taacggcagc tttaggctgt gccccagaca ttccgcccaa tccggaggat
    17641 acaaatacct tccctcccaa tgctttttcg ccgggtttag agtgcattcg tcctgcattg
    17701 ataaccgtta tggtggtgcc gtgaacaatg ccctgagggc ctatatacat gtaggaacct
    17761 gcggtcattt gcccatactg cgtaacgcca agagcgttaa aacgctccca gtcgtcctta
    17821 gaggagtagt tgggaaccat catcccgttg gtaattacca ccctgggggc atctggaaat
    17881 gatgggaaaa gtcccattgg atgtcctgag tacatcacaa gggtttggtg gtcggtcatt
    17941 tcggccaagt acttcatggt taacctgtac tgtgcccaat tttgaaaaat ggcgccatta
    18001 cctccgtaga tgataagctc atgggggtgt tgggcaacag ctgtgtcgag attattgctc
    18061 agcatcaaca ttatagaggc tgcctgcagc gatttgtggg gaaaatcgtt tattgtccgg
    18121 gcgaaaattt tatagccagg acggtaacgg tacatgtaaa ttcttccata ctcctcgagc
    18181 tctttggcaa attcggcagc taaaacagca tggtgtttcg ggggaaagta gcgaagagcg
    18241 ttcttaacag ccagcttctt ctcttcaagg gtaagaattt tctttcgttt gggcgcatgg
    18301 ttaacgtccg ggtcccatgg ctgaagggcg ggtaactcgt cggggatgcc ctgaagtatc
    18361 tccttttgaa attcttcgcg tgtcatactt tttgaatgtt tttaacctag tgttttgcca
    18421 caaatttaag attttaaacc gactttgaac ggcttacccg aaattgtaaa ttatattaat
    18481 ttaaccggta tgcatttttt catttgataa ataaagctac ttttggcaag tttaaaacat
    18541 atcaaattaa agaactatga agagtaaaac atggataacc attggaatag tagtgttagt
    18601 ggctgttata ctatatattt ccattaaagg atcgtacaac aaaatggttc aaaaggacga
    18661 ggcagttacc tcgcaatggg ccaacgttga aaatgtatac cagcgcaggt tagaccttat
    18721 acccaacctg gtgaatacgg tgaagggcta tgccaacttt gagcagcaaa ccctgaccca
    18781 ggtagtagag gccagagcac atgccacaca ggtaaaggtt tcaccggaga atctgaatca
    18841 ggagagtatc cagcaattcc aagccgctca gaacgagctg agtggggcgc tttcgcgctt
    18901 aatggtggta gtagagcagt accccgagct taaggccaac cagagctttc tcgaccttca
    18961 ggcacagctg gagggaacag agaatcgtat agctgttgag cgcaataagt tcaacgaagt
    19021 ggttagggac tataacacct acataaggcg attccccagt aatatttggg cagcactatt
    19081 caacttcgat aagaggggat actttgaatc cgaggccgga gccgaaaagg cgcccaaagt
    19141 tgattttggc acaaactagc caaatatata gagatagctg gccacaactt tacgctggca
    19201 caaccatgcc catcaatcca aaaaaatgaa ggcaaaggat tactttactc tcgaggagca
    19261 aaaaagaatt acagacgcgg ttgagcaagc cgaaaaaaac acttcaggcg aaatcagggt
    19321 gcacgtggag aacgaatgcc gaattgatgt gctcgacagg gccgttcaaa tttttgccat
    19381 gctaaaaatg cacaaaacaa agctgagaaa cggcgtgctg atctacctct ccctaaagga
    19441 ccacaagttt gccatactgg gcgatgccgg aataaatgca aaggttcctg aagatttttg
    19501 ggattcaacc aagcaggcga tgcttgaaga gtttcaggaa aacaggctga ctgaagggat
    19561 aattaaggga ataaccatgg ctggcgaaaa gctcaaggag catttcccct acaagcaaga
    19621 cgatgtagat gaactcacca acgatatttc gtttggctaa acccattctt cacattaagc
    19681 aacccaacca tgatcaccaa acaactcagg aatattcttc tagcggcggc gctattgctt
    19741 ccaaatattt cgtggagcca ggatattccc gaccccatga atccaccccg gctggttaat
    19801 gattttgccg ggattctcgg aggcgaagag cgggatgcac tcgaatcaat gctgcgtggc
    19861 taccacgact ccacctcaac ccagatatat gttgtaacag taaacgatct atctggctac
    19921 gacatatccg actatgcttt ccgcctgggc gaaaaatggg gagttggcca aaaaagcaaa
    19981 aataatggcg tggttatcct gattaagcct aaggtaggga atcagaaagg gcaagctttc
    20041 atagccaccg gttacggaat ggaggctata attcccgacg ccgttgctcg cagaattgtg
    20101 gacaacgaga tgattcccta cttcaaaacg aacagctact acaaggggat tgtggcgggc
    20161 tgcaaggtaa taatcgacct ggccggagga gagtacactg ctgatgctta ctacaaaaag
    20221 gaatcgccta ttccgggaat tatcctcctg atcatatttt ttggattcat attcctttcg
    20281 ggcggcaggc ggtcaaagct acgttcagga tctattggga aaaacattcc gttctggatc
    20341 ctactaggct caatgggcag cggaagacgt ggtggtggat ttgggggatt ttcttccggc
    20401 tcgggcggat ttggaggatt tagcggagga ggcgggggta gcttcggcgg tgggggtgcc
    20461 ggaggaagct ggtaacctgc cgatccttat tctttgcagc taatcatctc aactgtacat
    20521 tctactctgt actctccgtg gttatgtctt tctcaccaca gagagcacgg agcagcacag
    20581 agagagttaa agtttctgct ctgtgtacct ctgtgtcctc cgtggttggg cttttcttac
    20641 cacagagagc atggagcagc acagagaaag ctaaagtttc tgctacgcgt tcctctgtgt
    20701 cctccttggt tatgtctttc tcaccacaga gagcacggag cagcacagag aaagctaaag
    20761 ttcctgctct acgtacctct taaatatttc tttttatttc aaattatcca acgaactatg
    20821 ccatccttta atcgtaaaac attgaagttt attaataatc caagtttaat gttggccagt
    20881 ttcatatagg tcatcagttg tgctttatgt atatcagcga tagcatcaac tgattttatc
    20941 tcaataataa ccttattctc aaccagtaaa tctatacgat agcctgcatc taacttaatt
    21001 tctttataaa ctaatggtaa tgcgagttgc ttttttacat taaggccttt gtttattaat
    21061 tcgtaaaaca aacactcttc ataagcactt tcgagcaaac ccgctcctaa ttgtttatgt
    21121 acttctatag cacatccaat tattttttct gttatcagat tctcttccat ttctctatat
    21181 ttttttctcc gtgtacctct gtgtcctccg tggttgggct tttcttacca cagagagcat
    21241 ggagcagcac agagagagtt atattttcaa ctcctctatc gcatctttaa acttcggcaa
    21301 ctctcctata cgcatgatga ttctacaatt ggtttttaaa caattccgag aataattcgt
    21361 acatcatgcc gctagtaatt ttgcaatttt tctgtttggt tttgagttat atcaatataa
    21421 aatcccaatt tcagagcatg ctctacgacc atattctgtg taagtaacaa tttttactag
    21481 ctcccagata cgaaaccttt cagggaaaat ataggaatat agtggttcag ccaatcattc
    21541 aggaaaacca ggctcaaaca agcgtaactt gaacacccgg aagtgaatcc gaaaagctgc
    21601 attcttacta taaccttcaa ctacaatgct caatttcact acgcctacgg agaatccctt
    21661 tcaccctttc gagggttatt tttgctatcc ctgcttctaa aggtcaggta aaatgaaatc
    21721 cataaaagca taattgttcc gggcagaata acgccacgcc cggtataaaa gtaggagagc
    21781 gataaaatca tacagttata tggattgtcg gggttgtaaa taactgtaaa cctacgtcca
    21841 acttcatagt tggtattctc ggggccatac attcttagtg ttagagaatc tgcattaaac
    21901 tcaataaccg ggtaggagtc gtaaccatgc gactgataaa aagcagatga cttcacctcg
    21961 ttggcaacca ccacgccttg cgttcgttta ccaaagatga gcaaccggta ctttgaagaa
    22021 atgggtagaa ggactatgag aaaggttatg aaccagaagt tcttacgact aatgcgaaag
    22081 ctaacttccc gataggccat ttgttaggtt ttgcttttgc tcaaggcttc attatagcag
    22141 tgcctgcaaa ggggttcgta tatgtcggtt tcgcccagca ccactagttt atcggtgtcg
    22201 gcagttcggt gcgagaaatg agccagctcg ccacagcgaa cacagatggc atgaaccttg
    22261 gtaacatact cggcaatagc catcaggttt ggcattgggc caaagggttt accacgaaaa
    22321 tccatatcaa gccctgccac aattaccctt acccccatgt cggcaagctg ggtgcaaacc
    22381 ctaggtagtc ccatatcaaa aaactgcgcc tcatctattc ctaccacatc tacatcctgt
    22441 gccagcagta aaatgttgcc cgatgaagta accggcgtgg agataatgga gttggcatcg
    22501 tgcgatacca ccttctcctc ggagtaacgg gtatcaattt ggggcttgaa aatttccacg
    22561 cgcaacctgg catactttgc tctctttagt cggcggatta gctcctctgt tttacccgaa
    22621 aacatcgaac cggcaataac ctctatccat ccattgcgct tgttgcttac agaattttcg
    22681 ataaaaggca tgggtctaaa atgaattatt agcgttagtt ttacatacaa atttaataaa
    22741 acttattttt gaaggtcgaa aaatcttaac agttgacagt gatgcaatat cttcgagact
    22801 cgaaaacact tttagacgac agcatgaaaa taattgaaag agccctggca tccgaaaatc
    22861 tgccgtcaat tgaacgtaac atactgcttg acaaacttgc caaagcctac gagctgctgt
    22921 tgtttagcgt tgaaaagcag gctgaaccta cccatcaagc ccatagccag cataccgtta
    22981 aggcaaatgc cgaaacttca actcaaggca tcaaaagaga tcagccttta tccaccccaa
    23041 gccataatgt tgaacctgtt gctccagctg gttcatactc aaagaaacca cccgagcaaa
    23101 tacaaaagtt cagggaaaag gctccatcta caaatgaaag taaaagtatt gaggttgagc
    23161 caacccccgg agcagccagc accgatcgac cggcaacgtt ggccgataag tttcaggaca
    23221 agcaaaagtc acttaacgaa actattgcca accaagcgcg caggtccgat atgggcacca
    23281 agctgcagaa caagcccatt accgaccttg ccaaggcaat aggcattaac gataagttcc
    23341 tgttcaccaa cgagctgttt gatggcgatt cggcgctcta tgccgaaacg gtaaaccgtc
    23401 ttaacgagat ggacgacttg aacgaggcca ttatttacct tcaggataac tttagctgga
    23461 gtgcaaccag cgaagctgca tcaaaattta tcgatttatt gcgcaggaag tttgcactgt
    23521 aactacctcc tgcccttccc tgttactttt tttagtaatg ttcatttttt aacatttgat
    23581 agcgctggca gatgtaaagc cccacccacc caaaatgttt gaaattgaaa cttcaccaaa
    23641 gtaaaaaaac acgctaacct ccaacccatg tccaaactat tcattgttcc tacgccaata
    23701 ggaaatttag gtgatataac gttaagagcg ctggaggtac tacgttcggt ttcgctagta
    23761 cttgcagagg atacccgcac cagtgggaat ctgcttaagc attacagcat cgaaacccca
    23821 ctaagatcgc atcataagtt caacgagcac caatcggttg aagcaattgc cgaacagata
    23881 gccgccggac ttgacgttgc cctgatatcg gatgccggaa cacctggcat ttccgatccg
    23941 ggtttcctcc tcgtgcgcac ttgcctggaa cggggagttg aggtggaatg cctacccggg
    24001 gcaacagctt ttgttcctgc gctggttcag agcggtatgc cctgcgatag atttgtgttt
    24061 gaagggttcc tgccacagaa aaagggtcgc agcaagcgaa ttgaggcact tcaagacgag
    24121 gatcgcacga tggtgtttta cgaatcgccc tttcggctgg taaagctgct ggagcagctc
    24181 atggctgttt tcggccccga ccggagggtt agcgtttcga gggagctgac caagctgcat
    24241 gaggaaacaa tgcggggaac actagccgaa gtggcagcac acttcaactc gaaaaccgtt
    24301 aagggtgaag tggtagtggt tgttcagggt ttaggaagga ataaaagcct tgacgaggag
    24361 taagcaataa aaaagccccc aagactaaac cgcttggagg catacgaagg ttgtttctat
    24421 tacatgctgg ccaaggagtc gtccaccaga attcggccac agtactcaca cacaataatt
    24481 ttcttatgaa tctttatgtc gagctggcgc tggggaggaa tcttgttgaa gcaacccccg
    24541 caggcatcac gctgcacggt aacaacagcc aagccgttgc gtgcatttcc cctaatccga
    24601 cggtatgctg tgtaaagcct gggttcaata atctttccat actcctcgga tttcctaata
    24661 agctcctctt cctccttctg ggtctcgttt ataatattat cgagctccat tctttttccc
    24721 tttagatcga gttttcgctc ctcaagcaac ttttgcgact gctcaataac aagagtctta
    24781 tcctttacct gttgggtaaa ctccttaatt cgcttttcgc aaagttcaat ttccaatgtt
    24841 tggtattcaa tttcctttga gagcgaatca aactcccggt tgttcctaac agttttttgc
    24901 tgctcctgat atttttttat aagcgtattg gcatccttaa tttcctgttt cttggcagac
    24961 acctcgctct caaggctctt gatttcctgt tttaggttat caattctggt ttcgagccct
    25021 tcaatttcat cctccaggtc ctgaacctca aggggaagtt ctcccctcag cattctaatc
    25081 cggtcaatct tagaatcaac ctgctgaagc atgtacagaa aacgtaattt ctcctccacc
    25141 gaaagctcgg cggtaggttc agctggtttg ttcttcactg ctgccatatt ttacaagcta
    25201 taagtagtta attggattgg tatttaccct tgattttaag accgcaaagg tagggaattt
    25261 tttcaaaagg tactcataaa ataattcaat agtaaactgt tcgctctcga agtgcccaat
    25321 gtcggcaatg ataattctgc catcggcatc gaagaattgg tggtacttga tatctgaggt
    25381 aataaatatg tcggcgcccg acccaatagc ctttccaatc aggctggcac ctgaacctcc
    25441 gcagaatgca acccttttga taacttgacg tgaaa
//
LOCUS       DAOPHX010000019        24626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_2069_length_24626_cov_5.710594, whole genome shotgun sequence.
ACCESSION   DAOPHX010000019 DAOPHX010000000
VERSION     DAOPHX010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 24626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24626
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2069_length_24626_cov_5.710594"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PK502_02680"
     CDS             complement(<1..1128)
                     /locus_tag="PK502_02680"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPG99756.1"
                     /translation="MRLAMYFLPAAIFALSCNRQVVSEPELVERANELHKKIVTIDTH
                     TDAPLTFMRTGYDFSGKNNKAIGSCVDLAKMEAGGLDAAIFAVFISQDECTPDAYRKV
                     NAQALQIASTVINTVAKYPDRAEIAVNPEDIFRLKAAGKRAIYIGMENGYPLGEDLSQ
                     LKVFYDLGARYVTLCHTRNNQICDSSTDPEGSRNNGLSDFGRSVVAEMNRLGMLVDVS
                     HISDKSFYDVLAVSKVPVFASHSCARAICNNPRNLDDNMLKALAKNGGVVQMCILSDY
                     VKQGEKNPVRDSAFRALRRKYGNFRNLTPEQEEQAEADWHKLEQQYPAKLATVSDVVD
                     HIDHIVRVAGIDHVGIGTDFDGGGGVDGCRDASELHAITIEL"
     gene            complement(1413..3233)
                     /locus_tag="PK502_02685"
     CDS             complement(1413..3233)
                     /locus_tag="PK502_02685"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPG99757.1"
                     /translation="MEKKKIVDVHEVKKVVNLGPIVGDGVAHLIMSALRFNKLNEKYS
                     NISHLKGLEFLSAAMDTFEITYEVNPEELKRIPRTGAFISVSNHPFGGVDGMILIKMM
                     LEQQRTDIKILSNFIIQRIPQIGEFVFPVDPFDNGVKRGSNLIGLKKALTHVSNGGAL
                     GLFPAGEVSTYYDNMDEVGVVDRQWQPSMIKFIKRAGVPVVPIYFQGENSRIFHLLGL
                     IHPMLRTAKLPSELLNKKNQVIRVRIGYPISVEEQDAYCDLDRYGRFLRVKTYALGSS
                     LDVDKFLSYKLRSAPVPEPVIPPVPFSAVENEVEKLLSKYLLFRSKNYAVVCAPSVEM
                     PNLMTEIGRLREITFREVGEGTNRKIDVDEFDLYYWQLFIWDEEEKRIVGAYRAGKGK
                     EIIENYGIEGFYIQTLFKIDKEFAPILEQCIELGRSFIVPDYQRKPLPLFLLWKGILY
                     FLIKNPEYRYLIGPVSISSRFSDYSKGVIITFMKSNYFDHNFARFIRPRNSFRVPVKE
                     EEFKIIFDTSDDLDKLDKFIKEVEPYDYRMPVLLKKYLKLNGRILGFNVDPKFNNALD
                     GLLILDLLQVPIQTITSLSKEINDKSILERFNISDYTFED"
     gene            3651..4496
                     /locus_tag="PK502_02690"
     CDS             3651..4496
                     /locus_tag="PK502_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99758.1"
                     /translation="MKKFAVTLAILFGVANLYAQRNLVRPEDVKTFMASKTYVVLEEN
                     PTSEYNIEIREAVERSWKITPYEFITSKEFEKVRQDIDKSFLVLLQVRFDNDKVAPVY
                     NFLSVSLGAAVKKLTDMPDICSVPLSYKEVSEDTYTYKLEGLINFMQNYIRDINTNPK
                     LIKKDAFKLYNKNMKQIKNKELWVTESSLDKTTRALTDIRNNYKYSIKVVKPEDIQKA
                     ILERNPNVVFLHKVGPEGTRLQWRVWKMIIGAGDGMMYYYDMHTISKSAGDGFLVKDF
                     KKIGS"
     gene            4573..6246
                     /gene="ettA"
                     /locus_tag="PK502_02695"
     CDS             4573..6246
                     /gene="ettA"
                     /locus_tag="PK502_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HPG99759.1"
                     /translation="MADEKIIFSMVGVCKTFPPQKKVLNNIYLSFFYGAKIGIIGLNG
                     SGKSTLLRIIAGVEKSFDGEVVFSPGYSIGYLEQEPHLDESKTVKEVVQEGVAGTVKL
                     LEEYEELNARFAEPMDDEQMNRLIQRQGELTELIDHADAWNLDSKLERAMDALRCPEG
                     STPVKILSGGERRRVALCRLLLQEPDILLLDEPTNHLDAESVQWLETHLQQYKGTVIA
                     ITHDRYFLDNLAGWILELDRGEGIPWKGNYSSWLEQKTQRLAQEEKQESKRRKTLERE
                     LEWVRMSPKARQAKSKARLSAYDKLLNEDVKQKEEKLEIFIPNGPRLGNLVIEAKDVA
                     KSYGEKLLFEHLNFILPPNGIVGVIGPNGAGKTTLFRLIMGMETPDYGEFRIGDSVKL
                     AYVDQQHQAIDPEKTVFEVISGGLDSMMLGSRTINARAYVARFNFSGADQEKKCGMLS
                     GGERNRLHLALALKEEGNVLLLDEPTNDIDVNTLRALEEGLEDFAGCAVVISHDRWFL
                     DRIATHILAFEGDSQVYFFEGSYTDYEENKRKRLGDEGPQRIKYRKLKE"
     gene            complement(6418..7437)
                     /locus_tag="PK502_02700"
     CDS             complement(6418..7437)
                     /locus_tag="PK502_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99760.1"
                     /translation="MKARYLIIGAVLLPVASYGQKQLQPTIAAMKWGVDYKLHVRLAN
                     DSAYVVDVRGLYHTGRDLFVDTTDNSATYYPVTLDREFVDYIKRKKIDTSDSLSLADS
                     NRTASYKTLWSALHSNLGGGYIHFVNCLIYALESQNLKLNDPIFKRPATRWKPNPVTQ
                     SYKRTHKWSYFYPDNQKLAQKEYRIRLKENDLKDLQGVPLQFTNLFLHTSQKKYEELV
                     RQQKNREVAQINLIRLLLGAKYLGEDQIRHISNSVISAVLKYNVNTLPSIIIFDDYNA
                     AVALTLDMNGYRIDRIVYSDGEVISGKELEGRTAIIDGLIRNINEANERVFQKKLQLY
                     YQKTR"
     gene            complement(7596..9338)
                     /gene="recJ"
                     /locus_tag="PK502_02705"
     CDS             complement(7596..9338)
                     /gene="recJ"
                     /locus_tag="PK502_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPG99761.1"
                     /translation="MREGSTMDKRWVFKDVDGDEAVNKLAAELNIPHTLASLLVQRGI
                     KTFDEAKRFFRPSLDDLYDPFLMKDMKEAVDRIVTAIHHGESILFYGDYDVDGTTAVA
                     LLYSFFRQRYSKIGYYIPDRYGEGYGISYKGIDYAFENGYSLIIALDCGIKANEKVLY
                     ASQRKIDFIICDHHFPGDQVPGAVAVLDPQRPDCHYPYKELSGCGVGFKLLQGYCLKT
                     GTPMQELFQHIDLVAVSIASDIVPITDENRVLAYYGLEKINSNPSKGLKTIIQIAGID
                     KQKIAIDDIVFRIGPRINAAGRMESGKSAVDLLCAADDGTAYEMGSLVNSCNNDRKNV
                     DRSITHEALRMIAADARLQHCHSTVLFNPSWHKGVVGIVASRLIETYYRPTVVLTQSG
                     DLATGSARSVPGYDLYQAVEACSDLLENFGGHMYAAGLSLKLENVENFKNRFEEYVSQ
                     TITPELMMPQIEIDARIKLSDINDKFYRILKQFQPFGPGNMSPVFAAENVRDNGECRL
                     VGPDKEHLKLSLAEEGNPNIYHGIAFQLGEYYRDIHNGKSFNVCFNIYENVYRGITTL
                     QLRVKDLKFIESQG"
     gene            complement(9313..10050)
                     /gene="lipB"
                     /locus_tag="PK502_02710"
     CDS             complement(9313..10050)
                     /gene="lipB"
                     /locus_tag="PK502_02710"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HPG99762.1"
                     /translation="MQNVIFTDLGTIDYKEAWDLQEKLFDEVSLNKAEGDGNLNFLLF
                     CEHPHVYTLGKSGQRSNLLVSDEMLKRINASFYHINRGGDITYHGPGQLVAYPIFNLE
                     AFGLSLKSYISLIEQVVIDCLGDFGVPANRLDGATGVWLDAGIKGKERKICAIGVRAS
                     RFVTMHGLAFNINTNLDYFGYINPCGFTDKGVTSLQRELGEEVNPEMVKQRILDHFAR
                     KFNFNVNTRLFPNLKLATNYEGGKHNG"
     gene            complement(10090..10962)
                     /gene="lipA"
                     /locus_tag="PK502_02715"
     CDS             complement(10090..10962)
                     /gene="lipA"
                     /locus_tag="PK502_02715"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPG99763.1"
                     /translation="MTEVRKPAWLKVKLPGGEGYRRVDSIVKNNSLHTICSSGMCPNI
                     GECWGNGVATFMILGDICTRACKFCATTTGRPLPPNPNEPEKIARSIQLMALNYCVIT
                     SVDRDDLPDQGAAHWRNTVNAIRRVNPNTMIEVLIPDFDGRSDLIDIFLESNPNVVGH
                     NLETVERLTPEVRTKARYRTSLETLSYIASRGFTTKSGIMLGIGETFDEVVQTLDDLS
                     AVGCRMITIGQYLQPRPNNLPVQRYVTPEEFEEYARIARQKGFVFAEAGPMVRSSYHA
                     DKAQKLFGTRPDSV"
     gene            complement(11334..11984)
                     /locus_tag="PK502_02720"
     CDS             complement(11334..11984)
                     /locus_tag="PK502_02720"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG99764.1"
                     /translation="MQGEEKVVSRYITNLIGMGEGLTLDFKHSITDSKKIARSLAAFA
                     NTQGGTLLVGVKDNGSITGVDTDEEYYMVEAASTLYCRPEVPFEVVKWNMNAKTVLEV
                     KVKPSKLRPHKAPDKNGHYKAYIRIADENMVVTSTQLKIWKAERSRKSIGLNLSNAQT
                     ALFEHFKQNPRITIDEFCKIGFLPATEAERILVNLTVLGTIRYSGNPADEYFYPSV"
     gene            complement(12010..12585)
                     /locus_tag="PK502_02725"
     CDS             complement(12010..12585)
                     /locus_tag="PK502_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HPG99765.1"
                     /translation="MGRKIVVGVTGASGAIYARSLLEILGELSDQLEEVALIFSDNGR
                     AVWEFELGHIPPFSAPFRVYDNNNLFAPVASGSAGFDTMFIAPCTMGTLGRIAAGTSS
                     DLICRSADVMLKERRRLIVVPRELPLSLIHIENMRTVTLAGGIIVPASPSFYSRPSSM
                     EELVRTVVFRMIDLAGLELPHYRWGEAKSDS"
     gene            complement(12829..14388)
                     /locus_tag="PK502_02730"
     CDS             complement(12829..14388)
                     /locus_tag="PK502_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPG99766.1"
                     /translation="MAKDIILELTPENAANSQLLLKAVASKLGMSVKDVGHYTITRRS
                     IDSRHGKVRVNLGIRVFDRDEPIADGKPQFTYGDVSKSPPVAVIGAGPAGLFAALRLI
                     EHGYKPIVVERGKEVSARKRDVALLNREHKLNPDSNYAFGEGGAGTFSDGKLYTRSRK
                     RGDYNRVLYILCNHGASEDILFDSHPHIGTNRLPGIIVKIRETIISYGGEILYNSRVE
                     SIKTDGSRVTGVKLSDGRLVEAKACILATGHSARDVYSMLLNQQIQIEAKPFAMGVRV
                     EHPQAIIDSIQYHGTPRGDYLPAASYSLVQQVEGRGVYSFCMCPGGFIVPAATEPNEV
                     VVNGMSPSLRNSRWANSGIVVEVRLDDLKSYSKYGELAGVEYQHYLEELASLHGGFGV
                     KAPAQRLVDFVDGNYSASLPSCSYHPGITSSELHAWLPDDIGFRLRKGFRVFGARMKG
                     FLTNEAVVLGVESRTSSPVRIPRNPETLQHVGVEGLFPAGEGAGYAGGIVSSAFDGER
                     CADAVAFYLGG"
     gene            complement(14584..15324)
                     /locus_tag="PK502_02735"
     CDS             complement(14584..15324)
                     /locus_tag="PK502_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPG99767.1"
                     /translation="MKKIFVVIAVIWCSVTAAQAQYRKPNILNDPDYDVGKPLRFGFS
                     IGINIMDFDIKNSSVPELNADSSQFQYFTEVTHINPGLNVNAISDLRLAENFHLRFLP
                     GYSFGQRNLDFFKVENGRVTHETTMDIESNFIDLPLGIKYLAERTSNIRPYLYVGGDY
                     RIDLAAYKRLKVEKGILVRLQKQDFYYEIGFGVDFFFEYFKFSTEVKWSAGIGNVISS
                     DYAEGAENYRYAVDAMNSRLIMISFHFE"
     gene            complement(15578..16288)
                     /gene="ubiE"
                     /locus_tag="PK502_02740"
     CDS             complement(15578..16288)
                     /gene="ubiE"
                     /locus_tag="PK502_02740"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HPG99768.1"
                     /translation="MPTPDKSSQNVEAMFNSIASNYDFLNHFLSLGIDRKWRKRLIRL
                     VAKGNPQKVLDVATGTADLAMLLAREYGGVSITGVDISENMLAIGKRKVESENLSGQI
                     SLHRASALNLPFENDSFDAAMVAFGVRNFEDLSMGLSEVYRILINGGQFAILEFTIPD
                     KFPVAQLYRFYFRHVLPLFGGIISGSRTAYKYLPESVLAFPEKENFLEIMRQVGFTGC
                     TCKSLTFGIASIYTGFKP"
     gene            complement(16304..18547)
                     /gene="purL"
                     /locus_tag="PK502_02745"
     CDS             complement(16304..18547)
                     /gene="purL"
                     /locus_tag="PK502_02745"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HPG99769.1"
                     /translation="MIEHQAMQQHEVTMDTVRELGLLPEEFEMIKKFLGRVPNYTELS
                     IYSVMWSEHASYKNSIKWIKTLPRKGSALLAEAGAENAGLVDIGGGYACAFKIESHNH
                     PCAVEPYQGAATGVGGINRDIFTMGARPVAQLNSLRFGNPKLERTRWLMRGVVKGIGD
                     YGNAFGIPVLAGEVFFDDSFNTNPLVNAMSVGIVSKDSVVSAVAKGVGNPVYIVGSAT
                     GKDGIHGATFASANITENSADDIPSIQVGDPFQEKILLEATQEVIKTGALVGMQDMGA
                     AGIICSTSEMSEKGKHGMRIDLDKVPLRQTNMEAWEILLSESQERMLMVVKKGRERDV
                     EEILNKWDIGYAVIGNVTSGDSLEFYYHNELAAKVPASSLVLGGGAPVYDREYSEPEY
                     YKEFKKFNMNQIPEPADLVEVARFLTAHPNIASKRWVYQQYDTMIGTGNMTTNFPSDA
                     GVFNLKELNKALAMTVDCNSRYVKADPEVGTMIAVAEAARNIVCSGATPLAITDCMNF
                     GNPYNPEVYWQFVKAIKGMAKACKKFDTPVTGGNVSFYNQSSINGREEAVYPTPTIGM
                     IGMLENKNHHTTLAFEEKGHMIFLIGKSRNDIASSEYLYSYHGIKQSPAPFFDLDEEF
                     AIQQALLDLIARNLVQSAHDVSDGGLFIALLESAMPRNLGFDITTDAEIRTDAFLFGE
                     SQGRIVVSVSPSRETHFIDYMVEHGIPFTALGHVTKSEIRIDDVSFGFVADLKQLYDT
                     ALEKIMA"
     gene            18830..20554
                     /locus_tag="PK502_02750"
     CDS             18830..20554
                     /locus_tag="PK502_02750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPG99770.1"
                     /translation="MRTLNRFNKLILLVAALSYHQAAAQNGILKSPLDLKPALSGSFG
                     ELRTAHFHSGIDLRTNGKIGFRVYASEKGYVSRIKVSPVGFGKVIYITHPNGLTTVYA
                     HLDRFTNAVAEYVEKQQYASHSFEVELFPKPGEFNVSKGEVIGFSGNSGSSGGPHLHF
                     EVRSSATQWPVSPLKYLDGNFLADNSAPTIKSAWFYEIDSMDYLNDSILPNPISIKKT
                     GNLYFAPDTIRIAGKSRMGFGVEAFDIINGSSTTCGFHKLSMLVNNKQTFSFAVDSFS
                     YAETRCLNSIIDYSIRQLRQQEIVRLWADPGNLFSGLKADKSLGILTVESDSIYKISI
                     ELEDFYKNTSKLEMVVKGVHPFKNPESTPGLLRHSYVSWWNSYSFDSKDYSIYIPRRT
                     LYHDILLRHSALHTDSLAFPVISIHHARTPLNGKFTLRVKIAGVPANLRDKCFIGYIN
                     GKGIEYCSSRQDGPDMVTSPDKFGKFTLALDTVPPRIIPLNISQNADIAGQSSIKIKL
                     TDNYGIGTYSGFIDDGWVLFEWDPKTSLLTHTLTPKRERQNTWHTLRVEVADLLNNRV
                     TYKCRFYW"
     gene            21038..22423
                     /locus_tag="PK502_02755"
     CDS             21038..22423
                     /locus_tag="PK502_02755"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPG99771.1"
                     /translation="MNKRLLLLMLPVLAACGTKGPKEAVIAGKFTNSPDSNVYISTAG
                     ITDTVALDENGGFVYRAKLDKPQLYKLNIARTQTMVYLNPGDSSYMEFDVTSPVDGPV
                     FSADLKNIHQSIFSRRAALHNLMGSVNTLYSLSYDNFNRKLDSIQQVLYSMIDSASIE
                     PKDLAELEKTRTRYVILNVKANYPEYSAYLGGKEFNPDSVDYSFLNEVDPNNAYHLMF
                     DEYTSLVSIITQNQLVKKPGYKELLAKSSGERLPFEFKLIDSMLANPKVRDYLKMNKL
                     INDIEYGEFYELEGVVKSYMDSCQTPAYKQSVGNIFNQKMQLAPGKPAPIFVCKDING
                     KEYSLEDFRGKLVYIDFWATWCGPCRHELPYLEELQKDFKGKNIVFVSISLDDNKAAW
                     EKMVKEKQMKGVQLYGEDAWKSIPATSYQIKGIPTFFLIDAKGNIVKPNAPRPSSDEI
                     RPLITAELEKI"
     gene            22667..23002
                     /locus_tag="PK502_02760"
     CDS             22667..23002
                     /locus_tag="PK502_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPG99772.1"
                     /translation="MGVTKTEHYAEQQKAISELAKAMGHPARIAIVEHLVKVNACICG
                     DIVNELPLAQPTVSQHLKILKEAGLIKGEIEGNSICYCINQDAINRLQKYFDDLSAKL
                     NFKSNNCCK"
     gene            23014..23853
                     /locus_tag="PK502_02765"
     CDS             23014..23853
                     /locus_tag="PK502_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HPG99773.1"
                     /translation="MEDSEKIKEFVRQKYSEIALQDKETNQSSCCGSGCCSTEVYNIM
                     TEDYSTVEGYNPNADLGLGCGLPTKFAGIKKGDTVIDLGSGAGNDCFVARNETGADGR
                     VIGIDFTPAMIEKAKANASKLGFANVEFRLGDIERMPVSANMADVVISNCVLNLVPNK
                     DKVFNEIFRVLKPGGHFSISDVVLVGNLPDELCNAAEMYAGCVAGAIQKDHYIELIQK
                     NGFTNIAIQKEKPITIPDDILKSYLDEAALQNFKSGTTGIFSITVYAEKPAACGCGTG
                     CCS"
     gene            complement(24333..>24626)
                     /locus_tag="PK502_02770"
     CDS             complement(24333..>24626)
                     /locus_tag="PK502_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99774.1"
                     /translation="AEYLKEVGTYWTSPISKATNYSGFCALPGGASGTAPGSFLGKGR
                     ESVMWSSTIDYSLGDSIGWNIFWAILENNTVQSNIFMSRAGALNFLSIRCIKN"
BASE COUNT         6890 a         5844 c         5503 g         6389 t
ORIGIN      
        1 gagttcaatg gttattgcgt gtagctcaga ggcatcgcga catccatcaa cacctccacc
       61 gccgtcgaag tcggttccga tgcccacgtg gtcaattccg gcaaccctaa caatgtggtc
      121 gatatggtca accacgtcgg ataccgtggc aagcttagca gggtactgct gctcgagttt
      181 gtgccagtct gcctcagcct gctcctcctg ttcgggagta aggtttcgga agttgccata
      241 cttgcgacgc agcgccctaa atgcgctatc gcgtacagga ttcttctcac cctgcttgac
      301 gtagtcgctg agaatacaca tttgaactac cccgccattt ttagctaggg ccttcagcat
      361 gttatcgtca aggtttcgag ggttattgca aattgccctg gcacagctgt gcgaggcaaa
      421 aacaggcacc ttggaaacgg caagcacgtc gtaaaacgac ttgtcggaga tatgcgaaac
      481 gtcaaccagc atacccagcc tgttcatttc ggccactacg gatctcccga aatcgctaag
      541 cccattattc cttgaacctt cagggtcggt tgaggagtcg caaatctggt tgttccgggt
      601 atggcagagt gttacgtagc gagcgcccaa atcatagaaa accttaagct gcgataagtc
      661 ctccccaagc gggtatccgt tttccatgcc aatgtaaatg gctcgtttcc ccgcagcttt
      721 caaccggaaa atatcctcgg ggtttacagc aatttcagcc ctgtcggggt acttggcaac
      781 ggtgtttata acggtactcg caatttgaag ggcttgtgcg ttgacttttc ggtatgcgtc
      841 gggggtgcat tcatcctgac ttataaatac agcaaaaatt gcggcatcca gaccacctgc
      901 ttccatcttt gctaggtcaa cacagctacc aatggctttg ttgtttttac cgctgaaatc
      961 atacccggta cgcatgaagg tgagtggggc atcggtatgg gtatcaatgg ttacaatctt
     1021 tttatgcagc tcattggcac gctcaaccaa ctccggttca ctcactactt gcctgttgca
     1081 cgaaagtgca aatattgctg ccggcagaaa gtacattgct aatctcattt taccagaaaa
     1141 tttaaaaaaa gactaaagga taataaaaga aaatgcacaa aaaaactaca attccccata
     1201 tttaacagct gcgtaaactt tcaaaaaagc tgttttcaaa ctttatagcc agctatcgaa
     1261 cagaaaaatt gaccgaacaa atcggttatg gtttagctag aaaacatcaa gaattgaaaa
     1321 aaatcccctg tacccgtaca cgaaggggat tctacaaaca aacaactagc tccaataggt
     1381 taaacggtaa agcaggctac acaagctgca ttttaatcct caaaagtata atcgctaatg
     1441 ttgaacctct cgagaatgga cttatcgtta atttctttcg acagggaggt aatggtctga
     1501 atagggacct gaagaaggtc gaggattagt aaaccgtcaa gggcattgtt aaacttaggg
     1561 tcaacgttaa agcctaggat tctaccgttg agcttcaagt actttttaag tagcacgggc
     1621 atgcggtagt cgtagggttc aacctccttg atgaacttat caagtttgtc gaggtcatcg
     1681 gaagtgtcga aaattatttt gaactcctcc tccttcacag gcacccgaaa gctgttgcgc
     1741 ggacgaatga accttgcgaa gttatggtca aagtaattcg atttcataaa ggtgatgata
     1801 acccctttgg agtaatcgga gaaacggctc gaaatactca caggcccaat caggtagcgg
     1861 tattccggat ttttaatcag gaagtagaga attcccttcc agagcaggaa taggggcagt
     1921 ggtttgcgct ggtagtcggg cacaatgaag gagcgcccca gctcaatgca ctgctccagg
     1981 ataggggcaa actccttatc aattttgaaa agggtttgta tgtaaaagcc ttcaatgcca
     2041 tagttttcga ttatctcctt gccctttcct gccctgtagg cacccacaat tcttttctcc
     2101 tcctcatccc aaataaagag ctgccagtag taaaggtcga actcatccac atcaattttc
     2161 cggttggttc cctctcccac ctcgcgaaag gtaatctccc ttagccggcc aatttcggtc
     2221 atcaggttgg gcatctcaac cgatggggca caaaccactg cgtagttttt gctgcggaaa
     2281 agcaggtact tgctgagcag cttttcaacc tcgttttcca ccgcactaaa tgggaccggc
     2341 ggtataacag gttcgggtac tggcgcagac cttagcttgt agctaagaaa cttatccaca
     2401 tcaagcgacg aacccaaagc ataggtttta accctgagga atcggccata cctgtccaaa
     2461 tcgcagtagg catcctgctc ctccacgctg atggggtacc caattctcac cctgataacc
     2521 tggttcttct tattaagcag ctccgaaggt agcttggcgg tgcgaagcat agggtgaata
     2581 agtcccaaaa ggtggaatat acggctattt tcaccctgaa agtaaatagg aacaaccgga
     2641 acgcctgctc ttttaataaa tttaatcatg gatggttgcc attgcctgtc aactacacca
     2701 acctcatcca tgttatcgta gtaggttgaa acctctccgg cagggaaaag ccctaatgcc
     2761 cccccattgc taacatgagt taaagccttt ttgagcccta tcagattcga tccacgctta
     2821 actccgttgt cgaatgggtc aacagggaac acaaactctc ctatctgggg aatacgctgg
     2881 atgataaaat ttgacagaat ttttatatcc gtgcgctgct gctccagcat catcttaata
     2941 agaatcatac cgtcaacccc accaaacgga tggttcgaca ccgaaatgaa cgcaccggtt
     3001 cggggtattc gtttgagctc ctcggggtta acctcgtagg ttatctcaaa ggtatccatt
     3061 gctgccgaaa ggaactccaa tcctttcagg tgggatatat ttgagtactt ttcgttcagc
     3121 ttgttgaacc gcagggccga cattatcaaa tgggctaccc catcaccaac aataggccct
     3181 aggttgacca ccttttttac ctcgtgaaca tccacaattt tcttcttctc cattgctccc
     3241 aggttttctg aatttgaatg agaatgtatg cagataagtt gatatatagc aatttatact
     3301 ggtaactgcc accatttcca ccgttcaaat ctacataaaa tttaccgatt tagctttttt
     3361 gaacttagct ccgttcaagc ttaccctgga gggaaacatt tttttgtttt caggttagcc
     3421 ctaattttgt taaggaacct ttgattaaat catgaatttt taaaaaaaga agtataaatt
     3481 tgcaatctat aaatgcagag gtaaagaacc tggaatcatg cataatctaa ttttgccggg
     3541 ccaaatggta gagattaacc agcttcagaa aatttagttc aggttgactt ccggcgaatt
     3601 gctctttaag gaattatttg taaattacgt ggttaaaaat gtctaagctc atgaaaaaat
     3661 ttgcagtaac attagcgatt ctttttggag tggcaaacct ctatgcccaa agaaatctgg
     3721 taagacctga ggatgttaaa acattcatgg cgtcgaaaac ttatgttgtg cttgaggaaa
     3781 accctacatc ggagtataac atcgaaattc gtgaagctgt tgagcgctca tggaaaatta
     3841 caccctacga gttcatcaca tccaaggagt ttgaaaaggt gcgtcaggat atagacaagt
     3901 cttttctggt gctgttacag gttaggttcg acaacgataa ggtggcaccc gtttacaact
     3961 tcttaagcgt ttcgctaggg gctgccgtta aaaagttaac cgatatgccc gatatatgct
     4021 cagttccttt aagctacaag gaggtttcag aagataccta cacctacaaa ctcgaagggt
     4081 tgattaactt catgcagaac tacatcaggg atatcaacac caacccaaaa ctcattaaaa
     4141 aggacgcctt taagctctac aacaagaaca tgaagcaaat taaaaacaag gagttatggg
     4201 taaccgaaag cagtcttgat aaaaccaccc gtgccctaac tgacatcagg aacaactata
     4261 agtactccat caaggttgtt aaacccgagg atattcagaa agccattttg gaacgcaacc
     4321 cgaatgtagt attcctacac aaggttggtc ccgagggaac aaggctgcaa tggcgcgtct
     4381 ggaaaatgat tattggcgct ggcgatggga tgatgtacta ctacgacatg cataccatct
     4441 cgaaatcagc cggtgatgga ttcctggtta aggacttcaa aaagattggc agctaaatag
     4501 cattacacta accaaccgct ggttaacatg cttcgccagc ggtatttttt ataccctgta
     4561 acagcgaagt aaatggccga cgaaaaaatt attttctcca tggttggggt gtgtaaaaca
     4621 tttccacctc aaaaaaaggt gctgaacaac atctacctgt cgttcttcta cggcgccaaa
     4681 attggaataa ttgggcttaa cggctccgga aagtcaaccc tgctacgaat aattgccggg
     4741 gttgaaaaat cctttgatgg cgaggtggta ttctcgccgg gctattccat tggctacctt
     4801 gaacaggaac cacatctcga cgaaagcaag acagtaaagg aggttgttca ggaaggtgtt
     4861 gccggaacag ttaaacttct tgaagaatat gaggagttga atgcccgatt tgccgagcct
     4921 atggacgacg agcaaatgaa taggctcatc caaaggcagg gtgaactcac cgagttgatt
     4981 gatcatgccg acgcatggaa tctcgattcc aagctggagc gagccatgga tgcacttagg
     5041 tgccccgagg gcagcactcc ggttaaaatc ctctcaggcg gtgaacgtcg tagggtagcc
     5101 ctatgcaggc tgctgctcca ggaacccgat attctcctgc tcgatgagcc taccaaccat
     5161 ctcgatgccg aatcggtgca gtggctcgaa acccacctgc agcagtataa gggaacggtt
     5221 atagccatta cccacgatcg ctactttctg gataacctgg ccggttggat actggaactt
     5281 gacaggggtg agggcatacc ctggaagggc aactactcgt cgtggcttga gcagaaaact
     5341 caacgcctgg cacaggagga aaaacaggaa agcaaacgcc ggaaaaccct tgagcgcgag
     5401 ctggagtggg tacgcatgtc gccaaaagcc cgtcaggcta agtcgaaagc ccgtctatcg
     5461 gcctacgaca agctgttgaa cgaggatgtc aagcagaagg aggaaaagct ggagattttc
     5521 atccccaacg gaccacgctt agggaacttg gttattgagg ctaaagacgt ggcaaaatcg
     5581 tacggtgaaa agctgctatt tgagcacctg aattttatac ttccacctaa tggtattgtt
     5641 ggtgtaattg gccccaacgg agcaggaaaa acaacactat tcaggcttat catgggcatg
     5701 gaaacgcccg actatggcga gtttagaata ggcgattcgg ttaagctggc ctatgttgac
     5761 cagcagcatc aagcaataga cccggaaaaa actgtttttg aagttatttc gggcggtctc
     5821 gatagcatga tgcttggcag caggacgata aacgccagag cctatgtggc ccgcttcaac
     5881 tttagcggcg ccgaccagga gaaaaagtgc ggaatgctat cgggtggtga gcgcaaccgg
     5941 cttcatctgg cgctagccct gaaggaggaa ggaaacgtgc tgctgctcga cgaacccacc
     6001 aacgacattg atgtgaacac cctcagggcg ctggaggagg gtcttgagga ttttgccggt
     6061 tgtgcagtgg taatttcaca tgaccgttgg ttcctcgacc gcatcgccac ccacatactt
     6121 gcctttgagg gagattcgca agtttacttc tttgagggtt catacaccga ctacgaggaa
     6181 aataaaagaa aacggttggg cgatgagggt ccccaacgaa ttaagtaccg caagctaaaa
     6241 gagtagccaa ccctgaatgt taaatggagt gagcagccca gccatgaaac agcctatatg
     6301 gcggaatctt caaaaattga agtgaacgtt gtagcctcgc taataggcgt gagtacttaa
     6361 gctgctatgg agatcagcaa gccccgatta ttttacgaat ccaaggagag gctcatccta
     6421 tctggttttt tggtagtaaa gctgtaactt tttctgaaaa accctttcgt tagcctcgtt
     6481 gatattccta atcagcccat caataattgc agtgcgaccc tcgagttcct ttcccgaaat
     6541 cacctcgcca tcagagtaaa cgattctatc aatccggtag ccgttcatat cgagagttag
     6601 tgctacagct gcattgtagt cgtcaaaaat gatgatggag ggaagggtgt taacgttgta
     6661 cttaagcacg gcagaaatta cgctgttgct tatatgccta atctgatcct cgcctaaata
     6721 cttagcacca agcagcaacc tgatgaggtt aatctgtgca acctcccggt tcttttgttg
     6781 ccgaaccagc tcttcatact tcttttggga agtgtgcaga aacaggttgg taaactgaag
     6841 tggaacaccc tgaaggtctt tcaggtcgtt ttctttcagg cgtatcctgt actccttttg
     6901 cgccagcttc tggttgtcag gataaaaata gctccactta tgtgtacgct tgtacgattg
     6961 ggttacggga ttaggcttcc acctggtagc gggacgcttg aatatggggt cgttaagctt
     7021 taggttttgc gactccaggg cgtaaatcaa gcagttaaca aagtgaatgt acccaccgcc
     7081 aagattggaa tgcagcgccg accataacgt tttatagcta gctgtgcggt tgctatcggc
     7141 cagtgagagg ctgtcgctgg tgtcgatttt ctttctctta atgtagtcca caaactccct
     7201 gtcgagggtt accggatagt aggtggcaga gttgtcggta gtgtcaacaa acaaatccct
     7261 cccggtgtgg tataggcctc taacatcaac aacataggcc gagtcgtttg ccaacctaac
     7321 atgcagcttg tagtcaacgc cccatttcat ggcggcaatg gtaggttgca gctgcttctg
     7381 accatatgaa gcaacgggca gcaacactgc tccgattatc aagtaccttg ctttcacgta
     7441 attacttgtt tgggattcct ttataaaggt atagaattat ttgcttgatt tacctgatta
     7501 agccaaaaga agtattaaga ggagttagac tcagaatgca ccatgaagcc ggaatgctga
     7561 acgacttcaa aaaaccggca tcttgtctgc taaagttaac cctgactctc gataaacttc
     7621 aagtccttaa ccctaagctg cagggtggta atgcccctgt aaacattttc gtaaatgttg
     7681 aagcagacgt tgaacgattt tccattgtgg atatcgcggt agtactcgcc cagctggaag
     7741 gcaatgccgt ggtagatatt aggattgccc tcctcagcca gcgaaagttt aaggtgttcc
     7801 ttatcgggac ccaccagccg gcattcgccg ttatccctca cattttcagc agcaaaaaca
     7861 ggcgacatgt ttccggggcc aaaaggctga aactgcttaa gtatgcggta gaacttatcg
     7921 ttaatgtcgc tcagcttgat tctggcgtca atctcaattt ggggcatcat aagctccggg
     7981 gtgatggttt ggcttacgta ctcctcgaaa cggtttttaa agttctcgac attttcaagc
     8041 ttaagcgaca accctgcagc gtacatgtgg ccaccaaagt tttcgagcaa gtcggagcag
     8101 gcctctaccg cctggtagag atcgtagccc ggtaccgacc gtgccgaccc ggttgccaaa
     8161 tcacccgatt gcgtaagcac aactgtaggc ctgtagtagg tttcaatcaa cctcgaggca
     8221 actataccca ccacaccctt atgccacgaa gggttgaaga gaaccgttga atggcagtgc
     8281 tggagcctgg catcggcagc aatcatcctg agcgcttcgt gggtgatgct tcggtcaaca
     8341 ttctttcggt cgttattaca cgagtttacc aacgatccca tttcgtatgc ggttccgtca
     8401 tcggctgcgc aaagcaaatc aacagccgat ttgcccgatt ccatgcggcc ggcagcgtta
     8461 atacgagggc ctattcgaaa aacaatatcg tcaatggcta ttttctgttt atcaattccg
     8521 gcaatctgaa tgattgtttt aaggcccttg gatgggttgg agttgatttt ttcaaggcca
     8581 tagtatgcta aaaccctatt ttcgtcggtg ataggaacaa tgtcggaggc aatgctaaca
     8641 gccaccaggt ctatatgctg gaaaagttcc tgcatggggg taccggtttt aaggcagtat
     8701 ccctgaagca gcttaaaacc taccccacaa cccgaaagtt ccttataggg gtagtggcaa
     8761 tcgggccgtt gcgggtcaag aactgcaacc gcaccaggca cctgatcgcc agggaaatga
     8821 tggtcgcata tgataaaatc tattttcctc tggctggcgt atagaacctt ttcgttggcc
     8881 ttaattccgc agtcaagtgc tataattagg ctgtaaccat tctcaaaagc gtaatcaatg
     8941 cccttgtatg atattccata accctcgcca tacctgtcgg gaatgtagta gccaatttta
     9001 gagtaacgct ggcgaaaaaa agaatacaac agggcaacag cggttgtgcc atctacatcg
     9061 taatcaccat aaaaaaggat tgactctccg tgatgaattg ctgtaactat acggtcaaca
     9121 gcctccttca tatccttcat aagaaaagga tcgtaaagat cgtcaagaga tggtctgaaa
     9181 aaccgtttgg cctcatcaaa tgtctttatt ccacgctgaa caagtagcga ggcgagtgtg
     9241 tgaggaatat tcagctcagc agcaagcttg ttaacggctt catcgccgtc aacatccttg
     9301 aaaacccaac gtttatccat tgtgcttccc tccctcatag ttcgtagcta gcttcaggtt
     9361 cggaaaaagc cgtgtgttta cattaaaatt aaactttctg gcaaaatggt ctaaaattcg
     9421 ctgtttcacc atttcaggat tcacctcttc tccaagttcc cgctgtaacg atgtaacgcc
     9481 tttatcggta aagccgcatg ggttgatgta tccaaaatag tcgaggtttg tattgatgtt
     9541 gaaagcaagc ccatgcatgg taacaaaacg gctggcccgg acaccgatgg cgcaaatctt
     9601 cctctccttg cccttaatgc cggcatcaag ccaaacaccg gtagctccat caagacggtt
     9661 tgccggtact ccaaaatcgc cgaggcagtc tataaccacc tgctcaataa ggctgatgta
     9721 tgatttcaac gagaggccaa aagcctcaag gttaaaaatt gggtaggcaa ccagctggcc
     9781 ggggccatgg taggtgatgt ctccgcccct gttgatatgg taaaaggagg cgttgatacg
     9841 ctttagcatc tcgtcggaga ccaggaggtt gctcctttgg ccactttttc caagggtgta
     9901 aacatgcgga tgttcgcaga acagcaggaa gttcaggttg ccatcgccct ccgccttgtt
     9961 taggcttacc tcatcgaata gtttctcctg tagatcccat gcttccttgt aatctattgt
    10021 tccaagatcc gtaaaaatta cgttttgcat tatatgtatt ttttcttttt ctcatggtgg
    10081 gtaaagcatt taaacactat caggtcgagt tccaaacagc ttctgtgcct tatcggcatg
    10141 gtaggaggaa cgaaccatag gtccggcttc ggcaaataca aacccttttt ggcgggctat
    10201 tctggcgtac tcctcaaact cttcgggagt aacataacgc tgaacgggca ggttgttcgg
    10261 ccgaggttga aggtactggc caatggttat cattctgcag cccacagcgg aaagatcgtc
    10321 gagggtttga actacctcat cgaacgtttc gcctataccc agcattattc ccgatttggt
    10381 tgtaaaaccc ctgctggcaa tgtagctaag ggtttccagg ctggttctgt atcgggcttt
    10441 tgttcttacc tcaggggtaa gccgctcaac tgtttcaaga ttatgcccaa caacgttggg
    10501 gtttgattcc aggaaaatat caataagatc gctcctcccg tcgaaatcgg ggataagaac
    10561 ttctatcatg gtatttgggt tcaccctacg aatagcattc accgtgttac gccagtgagc
    10621 ggctccctga tcgggcaaat cgtctcggtc aaccgaggtg ataacgcagt agttcagcgc
    10681 cattagctga atggagcggg caattttctc tggttcattt ggattaggtg gaaggggtct
    10741 tccggtggtg gtagcgcaga atttgcatgc acgggtgcaa atatcaccaa gtatcataaa
    10801 tgttgccaca ccgtttcccc agcattcgcc gatgtttgga cacattccgc tggaacatat
    10861 cgtgtgtaag ctattattct tcactatgct gtcaacccgt ctgtaaccct cgcctccggg
    10921 caacttcacc tttaaccagg caggttttct aacttctgtc atccttcaaa atttttacaa
    10981 agtaaaaaaa aattggggtc tttcacgcta aaaaatacca ttgccctgta aatagacgct
    11041 aataaaaaat tatgctgatc ataatggttt catctgttca aaattttcag gtttccgatt
    11101 acagctactt tagctcatgc cttttaggca tttgggctga gcgaatgtaa cgcctaaatt
    11161 ttagcttcac cggacttgca agtaacgcct ggttgttcat acgtgcttat gcaaactgtg
    11221 tcctgcctgg gtacttcatt aaaaatgaag aaaggtcaac atgaactcta caaaagataa
    11281 accgtaagca gctattgcct ttagaccata aaaccataat aagcataaaa agtttagacg
    11341 gaagggtaga aatattcgtc agcaggatta cctgaatacc ttattgttcc taaaacggtg
    11401 aggttaacca gaatacgctc tgcctcagta gccgggagaa acccaatttt gcagaactcg
    11461 tcaatggtaa tgcgtggatt ctgtttgaaa tgttcaaaca gggctgtttg cgcattggat
    11521 aggttaagac cgatggattt gcgcgaacgt tccgccttcc aaattttaag ctgggttgat
    11581 gtaaccacca tgttttcgtc ggctatccgg atgtaggcct tgtaatgccc attcttatcg
    11641 ggggctttat gggggcgtaa ttttgaaggt tttaccttca cttccagaac ggttttggcg
    11701 ttcatgttcc atttaaccac ctcaaaaggt acttcgggcc tgcagtaaag tgttgaggca
    11761 gcttcgacca tgtagtactc ctcatcggta tcgacacccg taatgctgcc gttatccttt
    11821 accccaacca gcagcgtccc gccctgggtg tttgcaaatg cagccaatga acgggcaatc
    11881 ttttttgaat cggtgatgga atgcttgaaa tcgagggtca acccctcccc catgccaatt
    11941 aaattagtaa tgtacctact cacaactttc tcctccccct gcatggtaaa aaaattcgaa
    12001 tttatcaggc tacgaatcgc ttttagcttc tccccatcgg taatgaggca gctcaagccc
    12061 tgccaaatca atcatcctga aaacgacagt cctaaccagc tcttccatgg atgaagggcg
    12121 gctataaaac gatgggctgg ccggaacaat aataccgcct gcgagggtaa cggttcgcat
    12181 gttctcaata tgtataaggc tcaagggtag ctctctcggc actacgatca gccgacgtcg
    12241 ctccttgagc ataacatcgg ctgatcgaca aatcaggtcg gagctggttc ctgcagctat
    12301 tcttcccaag gttcccatgg tgcagggcgc aataaacatc gtgtcgaacc cagccgatcc
    12361 ggaggccaca ggagcaaaca ggttgttgtt gtcgtaaacg cggaaaggag cagaaaaagg
    12421 tggaatatgg ccaagctcaa actcccaaac tgcacgcccg ttatcggaaa aaataagcgc
    12481 tacctcctcc agctgatcgg aaagctcacc caaaatctct agcagcgaac gggcatatat
    12541 ggcaccgctg gcgcctgtta cccccactac aatttttcgt cccatgtcaa aatcaatagt
    12601 tagcagtccg gacgtttgcc tacagcattc tacttgttat attgccaaca aatgtatcaa
    12661 aaaaactttc aacttgttga ttttgttcac acgccaatcc ggcttagcgt gtcgttagct
    12721 aactatttac catggcgcta aggacattat gggtagctgc tttattccga tttaagcagg
    12781 aaggtgcatc acaatccatt tccagctcaa ggctttggtg aatcactttt aaccgcccag
    12841 gtagaaagcc acagcgtcgg cacatcgttc gccgtcaaaa gcagaagaaa ctataccacc
    12901 ggcatagccg gcgccctctc cggcaggaaa tagcccttcc acccctacat gttgaagagt
    12961 ttcggggtta cggggaattc tcacaggtga tgaagttctc gactccactc ccagcacaac
    13021 cgcctcgttg gtgaggaacc ctttcattct tgcaccaaat accctgaacc ctttcctaag
    13081 cctaaaccca atatcatctg gaagccaggc gtgaagttcg gaggaagtaa tgccgggatg
    13141 ataggaacag cttggtaagc tcgccgagta gtttccatcc acaaagtcaa ccagccgctg
    13201 tgcaggagct tttacaccaa aaccgccatg aaggctggcc aactcttcaa ggtagtgctg
    13261 gtactccaca ccggcaagct ccccatattt ggagtaggac ttcaaatcgt cgagcctaac
    13321 ctcaaccact atacccgagt ttgcccagcg ggagtttcga agtgaagggg acatgccatt
    13381 caccaccacc tcgttgggtt cagtggcagc aggaacaatg aaccctcccg ggcacatgca
    13441 gaacgagtaa acaccccttc cttcgacctg ctgcacaagc gagtaggatg ctgctggcaa
    13501 gtagtcgccc cgaggagtac catggtactg tatggagtca ataatagcct gaggatgctc
    13561 cacccgaaca cccatggcaa agggtttcgc ttctatctga atttgctgat tcaacagcat
    13621 ggaatacacg tcgcgagcgg aatgcccggt tgccagtatg caggccttgg cctccacaag
    13681 tcggccatcg gaaagtttaa cacccgtaac cctgctcccg tcggttttaa tactttcaac
    13741 ccgggagtta tatagaattt cgcccccgta gctaatgatt gtctccctga ttttcacaat
    13801 aatgccgggc aagcgatttg ttccaatgtg ggggtgcgaa tcgaaaagta tatcctcgct
    13861 agccccgtgg ttacaaagga tgtacaacac acgattgtaa tcacctcttt ttcgcgaacg
    13921 ggtgtagagt ttcccatcgg aaaaggttcc tgcacctcct tcgccaaaag catagttgga
    13981 atcggggttg agcttatgct cccggtttag cagggcaaca tcccttttgc gggcgcttac
    14041 ttctttgccc cgctctacca ctattggctt gtagccgtgt tcaataagcc ttaatgcggc
    14101 aaacaaccca gcaggacctg cgccaataac tgcaacagga ggcgatttgg aaacatcgcc
    14161 ataggtgaat tggggtttac cgtcagcaat aggttcatcc ctatcaaaaa ccctgatgcc
    14221 aaggtttacc cttacctttc cgtgtcgtga gtctattgac cgtcgggtaa tagtgtaatg
    14281 gccgacatct ttaacactca ttcccagctt actggcaact gctttcagca atagctggct
    14341 gttggccgca ttctcagggg tgagttcaag aattatatcc ttagccatgg tgaaaaatta
    14401 tcagcatcag tgtgaaaaaa aggaatttct ccaaacaata gtgttaagcc ccttctcaat
    14461 aatccctaaa acccggcgag gtgaacaatt tatagctgat tgagggtata tcaaaattcc
    14521 gcgtattacc ccaactacaa gaagatggat gaacagcctg ttttatgcta cacttccact
    14581 tttctactca aaatggaagg aaatcattat cagcctggag ttcatagcat caacagcata
    14641 gcggtagttc tcggccccct cggcatagtc gcttgaaata acgttgccaa tgcctgccga
    14701 ccatttcacc tctgttgaaa acttaaaata ttcaaaaaag aagtcaacac caaacccgat
    14761 ctcgtagtag aaatcctgct tttgcagcct aaccagtatt cccttttcga ccttaagccg
    14821 tttataggca gctaaatcaa tacggtaatc gccacccacg tatagatacg gcctaatgtt
    14881 gctggttcgc tcggcaaggt acttaatgcc taaaggtaaa tcaataaaat tcgactcaat
    14941 atccattgta gtttcatggg taacccgtcc attttcgacc ttaaaaaaat cgagatttcg
    15001 ctgcccaaag gagtaacccg gcaaaaagcg taggtgaaaa ttttcggcta gccttaaatc
    15061 ggaaatagcg ttgacattta aacccgggtt tatatgcgta acctctgtga agtactggaa
    15121 ctgggagctg tcggcattaa gctcgggaac ggagctgttc ttaatatcaa aatccataat
    15181 gttgatgccg atggagaaac caaaccttag gggtttgcca acgtcataat cgggatcgtt
    15241 gagaatgtta ggcttacggt actgcgcctg agctgcagtc accgagcacc agataaccgc
    15301 tattacaaca aagattttct tcaaaatagt tataagctct tagggtaaaa catctacgta
    15361 aaaaaattac cttggatgaa tgactctcaa acggatactg cacctgaaca tttatgtacg
    15421 ataggtaaag cctgttatca aacgtgcagg gtataaaaaa tattgcaaac aatagcttaa
    15481 acagacgtca aacattcgtg gtagggtttc taatggtcgg ttgttgaata agcaatcagg
    15541 gcctctcccc tgaaatggct tcatgcacaa aatgaattta aggtttaaag cctgtatata
    15601 ttgaagcaat cccgaaagtt aaactcttgc atgtgcaacc tgtaaaccca acttgcctca
    15661 tgatttctaa gaaattctcc ttctcaggaa atgcaagtac cgattcgggc aggtatttat
    15721 atgctgtccg gctgcccgaa ataatccccc cgaacaaagg cagcacgtgc ctaaagtaga
    15781 acctgtaaag ctgggcaaca gggaacttat cgggaatggt aaactcaagt atggcaaact
    15841 gcccgccatt tataaggatt ctatatacct ccgataaccc cattgacaaa tcctcaaaat
    15901 ttcttacccc aaaagccacc atggcagcat caaaagagtc gttttcaaag ggtaggttca
    15961 atgcactagc cctgtggagc gaaatctgac ctgaaagatt ttcggattcg acctttctct
    16021 tgccaatggc cagcatgttc tccgaaatat ccacccctgt aatggaaaca cctccatact
    16081 cccgcgccag cagcatggcc aaatcggctg ttcccgtggc cacatcaaga accttctgtg
    16141 ggttaccctt agctaccaac cggattaacc gtttgcgcca ctttctatca attccgaggc
    16201 ttaagaaatg gttcaagaaa tcgtagtttg aggcaatgct gttaaacatg gcctcaacgt
    16261 tctggctgct tttgtcaggt gttggcataa agcgttagga atactaggcc atgatcttct
    16321 ccagagcagt gtcgtaaagc tgcttcaaat cggcaacaaa tccaaacgaa acatcgtcaa
    16381 ttctgatctc ggatttggtt acatgcccca gagcggtaaa cgggatgcca tgctcaacca
    16441 tgtagtcaat gaaatgggtt tcacgcgatg gagaaacgga cactacaatt cgaccctgcg
    16501 actcaccaaa caggaaagca tcggttcgaa tctcggcatc agtagtgata tcgaaaccca
    16561 ggttacgggg catggcactc tcaagaagag caataaagag gcccccatca gaaacatcgt
    16621 gtgcggattg caccaaattc ctagcaatca ggtcgagcag tgcctgctga atggcaaatt
    16681 cctcatccag gtcaaagaag ggtgcaggcg attgcttaat gccgtggtag ctgtagaggt
    16741 actccgatga ggctatgtcg ttgcgtgatt tgccaataag gaaaatcatg tgcccctttt
    16801 cctcaaaagc cagcgttgtg tggtggtttt tgttttccag catgccaatc ataccaattg
    16861 tcggagtagg gtaaacagct tcctccctcc cgttgattga cgattggttg taaaagctta
    16921 cgttaccccc ggttactgga gtgtcaaact ttttgcaggc cttggccatt cccttaatgg
    16981 ccttaacaaa ctgccagtaa acttcagggt tgtaggggtt tccaaagttc atgcagtcgg
    17041 ttattgccaa aggagttgca ccggaacaaa cgatgtttcg tgcggcttcg gcaactgcaa
    17101 tcatggtgcc aacctcgggg tcggccttaa cgtaacgcga gttgcagtca acggtcatgg
    17161 ccaacgcctt gttaagctct ttaaggttga agactcctgc atcggagggg aagttggtag
    17221 tcatgttgcc cgttccaatc atggtgtcgt actgctggta aacccatcgt ttcgaggcta
    17281 tgttgggatg ggctgtcaga aaccttgcca cctcaactag atctgcaggc tcaggaatct
    17341 gattcatgtt gaatttttta aactccttgt agtattcagg ctcgctgtac tccctgtcgt
    17401 aaaccggcgc accaccgcca agcaccagcg atgaggccgg aaccttagca gccagctcgt
    17461 tgtggtagta gaactcgagc gaatcgcccg aagtaacatt cccgataaca gcgtatccta
    17521 tatcccactt gttgagaatt tcctccacat ccctttcgcg acccttctta accaccatca
    17581 acattcgctc ctgcgattcg gaaagcagaa tttcccatgc ttccatgttg gtttgacgca
    17641 gaggaacctt gtccagatct atcctcatgc catgcttgcc cttttcggac atctctgagg
    17701 tggagcagat aatacccgca gcgcccatat cctgcatgcc cactagtgca cctgttttaa
    17761 taacctcctg ggtggcttca agcaaaatct tctcctgaaa gggatcgcca acctgtatgg
    17821 aaggaatgtc gtcggcagaa ttctcagtga tgttggccga agcaaaggtt gccccatgaa
    17881 taccatcctt gccggtagca gagcccacaa tatatacagg attgccaaca ccctttgcca
    17941 cagccgatac tacggaatcc ttcgacacaa ttccaaccga catggcattt accagagggt
    18001 tggtgttaaa tgaatcgtca aagaaaacct ctccggccaa aaccggaatg ccaaaggcgt
    18061 ttccgtagtc gccaattccc ttcaccactc ccctcattag ccagcgagtt ctttcgagct
    18121 taggatttcc aaacctgagg gagttaagct gggcaaccgg acgagcgccc atggtaaaga
    18181 tgtcgcggtt gatgcctcct acaccagtag cagctccctg gtagggctca acagcacaag
    18241 gatggttgtg cgattctatt ttgaaagcgc aagcataccc acctcctata tcaaccaggc
    18301 ctgcattctc ggctccagcc tctgccagca acgccgaacc cttccggggc agggttttta
    18361 tccactttat ggaattttta tacgaagcat gctctgacca catcaccgag taaatgctca
    18421 gctcggtata gttgggcacc cttcccagaa acttttttat catctcaaac tcctcgggga
    18481 gtaaccccaa ctcccttacg gtgtccatgg taacttcatg ctgctgcata gcctgatgtt
    18541 caatcataaa ttattgaata aaccttgtgc caaagataca cgacaatttt caatttacgc
    18601 aaggtgggga gaaaaaccga ggcatgcacc caccggctaa gaattgaatt gataacagtg
    18661 atccagaatg tcttgaacct gtcaaagctg aacaggtttt ctttactttt tacccattca
    18721 ctttaaccta gttaatacca gctgcttttc aaaaatatta gctgtttacg atgtccgcca
    18781 gactaaatat ccgtacataa tttatctttg agtgttttaa aaataagcca tgcgtacgtt
    18841 aaatcgtttc aataagttaa ttttacttgt agctgcccta agctaccatc aggcggctgc
    18901 acaaaatggt atcctgaaat cacccttaga cctaaaacct gcactatcag gctcttttgg
    18961 tgaattacga acagcacact tccactccgg catagatttg cggacaaatg gtaaaattgg
    19021 ttttcgggtt tatgcatccg agaaggggta tgtatcacga attaaggtta gcccagtagg
    19081 gtttggaaaa gttatctaca taacccatcc aaacggactt accacagttt atgctcatct
    19141 cgaccgtttt acaaatgcag ttgcagaata tgtagagaag cagcagtacg ccagccattc
    19201 ctttgaagta gagctatttc ccaaaccagg tgagttcaac gtgtcaaagg gagaggttat
    19261 tggcttttcc ggaaactcgg gcagctcggg tgggcctcat cttcactttg aggttcgcag
    19321 ctctgcaacc caatggcccg tttcaccatt gaagtacctt gatggtaact ttctggccga
    19381 taactcggcc ccaacaatca aaagcgcatg gttttacgag attgacagta tggactacct
    19441 aaacgatagc atactgccaa accccatcag cattaagaaa acaggaaacc tgtactttgc
    19501 tcccgacacc atcagaattg ccggaaaaag caggatgggt tttggagttg aggcattcga
    19561 cattatcaac gggagtagta caacctgcgg cttccacaag ttgagcatgc tggtaaataa
    19621 caaacagacc ttttcctttg ctgttgactc gttttcgtat gcagaaaccc gatgcctaaa
    19681 tagcattatt gactacagca tacggcaact tcgacaacag gaaattgtac gcctgtgggc
    19741 tgatcctggt aatttgttta gcggattaaa ggcagacaaa tcattgggaa ttttaacggt
    19801 tgaatccgac agcatttata agataagcat agaacttgaa gatttttata aaaatacttc
    19861 caaactcgag atggtagtaa agggagttca tccgttcaaa aacccggaat caacccccgg
    19921 ccttttgcga cacagctatg tgagctggtg gaacagctat agcttcgata gcaaggatta
    19981 ctcaatctac attccccgcc gaacccttta ccatgacatt ctcctcaggc attcagctct
    20041 acataccgac agcctggcat ttcccgtaat tagcattcac catgcgagga ctccccttaa
    20101 cgggaaattt accctaaggg taaaaattgc aggcgttccg gccaacttgc gtgataaatg
    20161 ttttattgga tacattaatg gtaaaggtat cgagtactgc tccagccggc aggatggacc
    20221 cgacatggtt accagccccg acaagtttgg gaagtttacc ctggctttgg acaccgttcc
    20281 tccacgcata attcctctaa atatttcaca aaatgcggat attgcgggtc aaagcagcat
    20341 aaaaattaaa cttaccgata actacggaat tggcacctat tcaggattca tagatgatgg
    20401 gtgggttctg tttgaatggg acccgaagac cagcctgcta acccataccc tcacacctaa
    20461 aagggaaagg caaaacacct ggcacaccct tagggtcgag gtagctgacc tgctgaacaa
    20521 tagggtaacc tacaaatgcc gattctactg gtaggcttat tctaaccact tgtgctttcc
    20581 ataggaatct ccataataaa ctgaagaaaa ggttgaatgc ttgtaatttt tttcaccctt
    20641 ccatggcaaa ataaagaata acattgggtt tgctcacttt aaagccggta acttatatgt
    20701 acctggtagt gaaatatagt ccgcaaaaac caacgttttt cccttacaac aacttgcatt
    20761 aacctatttt tatatttgct ggcattaaaa cctttggctg gctataaatt aaaccaatag
    20821 atattttata ctcaaacatt ataatactct aaaatgaagt atctttggta aatgatatgc
    20881 acctattggt agatgaacaa caaggtaacc ttaccagcaa attcaaacct aaaatttaag
    20941 ttctccggct gtacggttgg cacataaaaa ttttacaact ttaccctcaa taaaaatgat
    21001 tcatactcac aaacaaactc aaacaaactc aatgaaaatg aacaaacgcc tattgctttt
    21061 aatgcttccg gtattggctg cctgtggaac caaggggccc aaggaggctg taattgcggg
    21121 gaagtttacc aactcacccg atagtaatgt gtacatctct actgccggca tcaccgatac
    21181 ggtggcactt gatgaaaatg ggggctttgt ctatagggct aagctcgata aacctcagct
    21241 ctacaagctt aacatagccc gtacacaaac catggtttac ctcaaccccg gcgatagcag
    21301 ctacatggaa tttgatgtta cctcacctgt tgatggtccg gttttctcgg ctgacctgaa
    21361 gaacatccac caaagtattt tcagccgaag agctgccttg cataatttaa tgggaagcgt
    21421 gaacaccctt tactccctta gctacgacaa cttcaaccgt aagctcgact caatccagca
    21481 ggtgctgtac tccatgatag attctgctag tattgaacct aaagaccttg cagaactcga
    21541 gaagacccgc acaaggtatg taattctaaa cgtcaaggcc aactaccccg aatattctgc
    21601 atacctgggt ggtaaggaat ttaaccccga cagcgttgat tactccttcc tcaacgaggt
    21661 tgacccaaat aacgcctacc acctgatgtt tgatgagtat accagtttgg tatcaatcat
    21721 tacccagaat caacttgtga aaaaacccgg ctacaaggag ctattggcta aatcttcagg
    21781 tgaaaggctg ccttttgaat ttaagcttat cgacagcatg ttggctaatc caaaggtacg
    21841 cgactaccta aagatgaaca agctgattaa cgatatcgag tacggggagt tctacgagct
    21901 ggaaggagtt gtaaagagct acatggatag ctgccaaaca cccgcgtaca agcagagcgt
    21961 tggaaacata ttcaaccaaa agatgcagct ggcacccggg aaacctgcac ctatttttgt
    22021 atgtaaggac attaacggta aggaatactc cctggaggat ttcagaggga aactggttta
    22081 cattgatttt tgggcaacct ggtgtggccc ctgccgacat gagctcccct acctcgagga
    22141 gctgcaaaag gactttaagg gtaaaaatat tgtttttgta agcatttcgc tcgacgacaa
    22201 caaggccgcc tgggagaaaa tggttaagga aaaacagatg aagggtgttc agctttacgg
    22261 agaggatgca tggaaatcaa tacccgccac cagctaccag attaagggca ttcctacctt
    22321 tttcctgatt gatgccaagg gcaacattgt taaaccaaat gccccaaggc catcttccga
    22381 cgaaattaga ccgctgataa cagccgagct ggaaaaaata taaccgcatg gctgttgctc
    22441 agcaccagga ataaactaat taaaattaaa ccccgaaaca agttcggggt ttttttatta
    22501 cttttctttc agaaaaattg tagcaatccg ttgctacctg tcggccctgg cagctttaaa
    22561 caacatagtt cgggttattc aggttaaaaa ataattgaga gaaaaattga aagtatcctt
    22621 gtggacaaaa ttttttatcg taatattgca atatttaaat atatctatgg gcgttacaaa
    22681 aacagagcat tacgccgaac agcaaaaggc catttcggag ttggctaagg ccatgggaca
    22741 ccccgccaga attgccatag ttgagcatct ggtaaaggtg aacgcttgta tttgcggcga
    22801 tattgttaat gagctgccat tagcacaacc cactgtttcc caacacctta aaatacttaa
    22861 agaagcaggg ttgattaaag gagaaattga ggggaactcc atttgctact gcataaacca
    22921 agatgctatt aataggctgc aaaaatactt tgacgacctg tcagccaagc ttaacttcaa
    22981 gtcaaacaat tgttgtaagt aaaaaagaaa aaaatggaag actctgaaaa aattaaagaa
    23041 tttgttaggc aaaagtacag cgaaattgcc ttacaggaca aggaaaccaa ccaatcgtcg
    23101 tgctgcggtt ccggatgctg ctcaactgag gtttacaaca tcatgaccga agactacagt
    23161 accgtggagg ggtacaaccc caatgccgac ctaggcctgg gatgcgggtt accaacgaag
    23221 ttcgcaggaa ttaaaaaggg cgacaccgtt attgaccttg gtagcggggc aggaaacgac
    23281 tgttttgtag cccgaaacga aaccggagcg gatggacggg ttatcggcat tgactttacc
    23341 cccgccatga tagaaaaggc taaagcaaat gccagcaagc ttggtttcgc aaatgttgag
    23401 tttaggctag gcgatatcga acgcatgccg gtttcggcaa acatggccga tgtggtaatc
    23461 agcaactgcg tgcttaacct tgtccccaac aaggataagg tgttcaatga gatttttagg
    23521 gtgcttaagc caggcggtca ttttagcatt tcagatgttg tgctggtggg caacctccct
    23581 gacgagcttt gtaatgccgc cgagatgtat gccggttgtg ttgcaggagc aattcaaaaa
    23641 gaccactaca tagaacttat ccagaaaaac ggattcacaa acattgccat ccagaaagaa
    23701 aagcccatta ccattcccga cgacatcttg aaaagctacc ttgacgaggc cgcacttcaa
    23761 aactttaaat cgggaaccac aggaattttc agcattacgg tgtatgccga aaaaccggcc
    23821 gcctgtggct gtggaaccgg ctgctgcagc taaattttat tacccgtacc tcgggctact
    23881 atttcaaagt ggctatagta tgaatgagtc gcacaatggt tccaagctgt gcatacggga
    23941 gggtatgggg tgaatttttc ccccatcact ccccacaact tcggtcaagt ggcaaacaca
    24001 ttccggttta tcaacaatag gtttaaaacg cataatccag ccggattata aaaaaaataa
    24061 cttcgttaag tttccagccg gctgacggaa gggcttgcac agtagcgggg gttaaattct
    24121 gattttatca aaaatagata cacaatgcaa ttggggtatg ggctgctggg gagttaacat
    24181 ccagcggcgt tatccgccgt taaagattag ttaaaggtat taaaaattcc gtatcagcaa
    24241 catcagcccc aaatggctta accacgtttt aggtgctggt catttttcaa taagactttt
    24301 gttttctcag ataaatggtt ttagattgag ttttagtttt taatacaacg aatagataag
    24361 aaattcaggg ctccggctct ggacataaat atattacttt gtacggtatt attttcaagt
    24421 attgcccaga aaatattcca tccaatacta tcaccgagag aataatctat tgtagatgac
    24481 cacataaccg attctctacc ttttcctaaa aaagaaccag gagctgtacc tgaggcacct
    24541 cctggaagag cacaaaatcc tgagtaatta gttgctttac taataggaga ggtccaataa
    24601 gtcccaactt cctttaaata ttcagc
//
LOCUS       DAOPHX010000020        24357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_2115_length_24357_cov_7.261007, whole genome shotgun sequence.
ACCESSION   DAOPHX010000020 DAOPHX010000000
VERSION     DAOPHX010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 24357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24357
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2115_length_24357_cov_7.261007"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1940
                     /locus_tag="PK502_02775"
     CDS             <1..1940
                     /locus_tag="PK502_02775"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPG99775.1"
                     /translation="TGVNTEIGKIGKALESVKEEPTRLKREMAVLVKRLAVIGVILCL
                     LVVIIYTITRGDLLNGFLAGITLAMAMLPEEFPVVLAVFMALGAWRISRNNVLTRQPA
                     AIETLGSVTVLCTDKTGTLTQNRMTVTQLYNGTNHIAVTSGDTFTEPFHQVIEYAILS
                     SQVNPYDPMERAIISIGDKYLQNSEHIHKDWVMEKEYPLSKELLAMSRVFSNTGTGRR
                     VIAAKGAPEAIFDLCHLPAAEVGKHEKAVAEMASRGLRVLGVARAEISTENLPTIQHD
                     FNFEFMGLIGLSDPIRENVPEAVAECYRAGIRVIMITGDYPVTATNIGREIGIKNPEL
                     YITGQDLETMTEEELGERIKQVNIFARVVPEQKLKLVNALKRNDEIVAMTGDGVNDAP
                     ALKAAHIGIAMGEKGTDVAREASSLVLMDDNFASIVKAVRMGRKISDNLQKALAYIFA
                     IHVPIAGLSLIPVFFANLPLILWPVHIVFLELIIDPACSIIFEAEKEEKNVMTRPPRN
                     INQPFFGVGKILMSCTQGLGILAVSLLVYFIGFRMGYAEKAVRAFTFITLIVSNIAVI
                     LSNRSWSDSIFKIIMTPNKTVKWIVGGAVLFLLLIVNIPALQNLFLFDKIGWLELGIC
                     TLAGLSTIIWFELYKHFKRII"
     gene            2254..4203
                     /locus_tag="PK502_02780"
     CDS             2254..4203
                     /locus_tag="PK502_02780"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG99776.1"
                     /translation="MKGSLAAILLAGSLSLVTPRLWGQPLSLDSLLHELSTVKSSEGK
                     YKILSSIAISYSDSSYSKSLEYWLEALEVAKKLESSKYTADILHQIGFTYLKMGEPKS
                     ALANFQNAAELYVSLNDNADLAGVYNDMGLIYRSWGRYDSALEFYLKSYKLYERLANA
                     EGMAMASNSIGQIYYYRENYTKAVDYFSSYLEINQGLGNYRAVAGACNNIASAYMELN
                     HLDRALDYYMKAFKIYDSLGVKIGVGIIQDNIGSLLYSKGDYRNALLYHFNALQIFNE
                     LKSDTRKCLTLKNIGSVYLKQNRPDVAVAYLNQSLDMAVGTGQKDVARDVHQTLANAY
                     EVIGSFPKAYYHLKNYQVLKDSMLNAETIEKIETLQAQLEMENQAAQVSLVQHKLKLQ
                     RQVAWTVSGLLAMMGGVLVLLYRENKKKNAALAEEQKFKESTVKRLDAFLQNTLKVAD
                     DGTVGGFFSDSWKLQPANSTQGNCKLLSFKVNNSTLAFMQVSDSQRVSPNLITLCLCC
                     WVKSNAHRLMISWVNFMKEMRENFAADPLTSDLDPSQYCILPFTINGDFVLNLTEEGF
                     AVWHSNKLIFPKAFQCPELVKGDTLYLYFTTIGLPLPDREQREMEKIIGSVASMEFSL
                     QPQAMQNLLKTFELENFTYIFALRI"
     gene            4240..4968
                     /gene="pepE"
                     /locus_tag="PK502_02785"
     CDS             4240..4968
                     /gene="pepE"
                     /locus_tag="PK502_02785"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HPG99777.1"
                     /translation="MDSQSKKTVKMLLISNSTNAGEAFLEHPKEELKRFLNGTKRVLF
                     IPWAAVTFSYDEFERKVQERFSEFGVEVDSIHHYRNVKTAVQEAEAIVVGGGNTFKLL
                     KTIQQHDIVETVRNKVLDGTPYIGWSAGANVACPTICTTNDMPITEPDNFGAFSLIRF
                     QINPHYLDANPAGHAGETREQRIMEFLEMDPYVYVVGLREGSMILLENVALTLKGPKS
                     ARIFKKGIPPFEVNPGDDMDFLFE"
     gene            complement(5013..5525)
                     /locus_tag="PK502_02790"
     CDS             complement(5013..5525)
                     /locus_tag="PK502_02790"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HPG99778.1"
                     /translation="MDKGEANKRWYALYTKPRNEKKIYSRLVEKGVEAFLPLQKRLKQ
                     WSDRKKLVEEPLFRSYIFVRIDAREYYEVLNTPGIVRYITFEGKAVPIPDRQIEQVKQ
                     LLVQDLEIEPVEEQIPPGALVEVRFGNLMGITGELVEHAGKKKVIIRIDHVSHSLLVT
                     LPKDYVARAV"
     gene            5934..6152
                     /locus_tag="PK502_02795"
     CDS             5934..6152
                     /locus_tag="PK502_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99779.1"
                     /translation="MKSKKDFRKPVKNDSYDSDEVKMVKKLKPVKKEKNAKRSIFSEI
                     DELDDLVDFKGTVLDEKFEDYDEEEEEL"
     gene            complement(6221..6712)
                     /locus_tag="PK502_02800"
     CDS             complement(6221..6712)
                     /locus_tag="PK502_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HPG99780.1"
                     /translation="MQEKFRVDVIDQKILSYLVKNARIPFLEIARECGISGAAIHQRV
                     KKMEDAGIIAGSRMIVKPKALGFDVCAFVGVQLVSTNLYSGVIEALKLMPEVVECHFI
                     TGEYALLLKIYCRDNEHLMDVLVNTIQNIPGISRTETFISLDQAIERPFYVKDKSVVH
                     NEK"
     gene            complement(7097..7579)
                     /locus_tag="PK502_02805"
     CDS             complement(7097..7579)
                     /locus_tag="PK502_02805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG99781.1"
                     /translation="MDNVTFRSTPTPADKAGIREILSSSGFFHDFEIDVAIELVEEAL
                     AKGTESGYYFVFADHMGTTISYACFGPIPCTRHSFDLYWIGTHQDYRGKGLGKQVLQR
                     VENEIKQLNGRKIYIETSSKPMYEPTRQFYLSNAYENEAILKNFYDEGDDKVVYSKSL
                     "
     gene            complement(7596..8582)
                     /locus_tag="PK502_02810"
     CDS             complement(7596..8582)
                     /locus_tag="PK502_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPG99782.1"
                     /translation="MNRQTIHAVILYNELSESAGPDERDVLDQVALVAEMLNKLGIES
                     QRAEFSLNLQKVRDELSRLKPDFVFNLVESVNNRGNLIFLAPSLLNSMGIPFTGGSLE
                     TIFLTSSKTLTKQLMQDAGIPTPFWYQGNGSFIPDRDKRYIVKPIWEDGSLGLDEDSI
                     FEGSNPKLAEILNSIDYNTHFIEEFVDGREFNISILAGQNGPQLLPPAEIIFRNYAPD
                     KPHVVGYSAKWIEDSFEYINTNRTFDLSPNDQPLLSTMGDICLHCWKTFRVNGYARVD
                     FRVDNAGNPWVLEINVNPCISRDSGFYAACMQAGIPFDEAVRSIIADIPFQI"
     gene            complement(8579..9562)
                     /locus_tag="PK502_02815"
     CDS             complement(8579..9562)
                     /locus_tag="PK502_02815"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPG99783.1"
                     /translation="MTVGLTYDLKSDYLKNGYSHEEVAEFDSDETIDSIESTLQSLGY
                     FTERVGNIINLTHALANGKRWDMVFNIAEGLHGIGREAQVPAILDAFQIPYVFSDPLV
                     LALTLHKGQAKRVVRDCGVPTADFAVITSLSEVEKIALPFPLFVKPAAEGTGKGINGQ
                     SKINSKEVLYATTARLLEEYRQPILVETYLPGREFTVGIVGTGDKARCLGVMEIVISN
                     RAKDGTYSLHTKENWKGIVDYYMATGEIESLCAETALMAWNCLGCRDGGRVDLKMDAS
                     GKPNFIEVNPLAGLNPFHSDFPMLAQKNGIDFTMLIQLIMDSAMERFNHTA"
     gene            complement(9737..11014)
                     /locus_tag="PK502_02820"
     CDS             complement(9737..11014)
                     /locus_tag="PK502_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPG99784.1"
                     /translation="MSKELMHETLNTLGLEEDDEPPGSGQLSPNYGLITATSHQHKSP
                     SCNWPFPISKRSLEFLIKYFPNATVEDWMSWKWQLKNSFTTSEKLKCFLDLSREECEA
                     IDHPKHSLPLRITPYYASLLNGKNSQDPLRRTMIPTVNELTTSAGESCDPLNEGHSSP
                     VPNLVHRYPDRVLFLSTGFCSAYCRYCTRSHMVAKDKCHVGVNAWLPAINYIREHTEV
                     RDVLISGGDPLTMSDAHIEYLLSQLRSIPHVEMIRIGTKVPVVLPHRITPKLIKILRK
                     FHPLYISIHFTHPDELTPETAEACRRLADAGIPLGSQTVLLKGINDNVDTMKSLFQGL
                     LKIRVRPYYLYQCDPIPGSSHFRTSVEKGLEIIKGLRGWTTGYAIPHFVIDAPGGGGK
                     IPLLPEYYQGRNEEGYVVLKNYEGKTFYYPDLH"
     gene            11271..11345
                     /locus_tag="PK502_02825"
     tRNA            11271..11345
                     /locus_tag="PK502_02825"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11304..11306,aa:Arg,seq:cct)
     gene            complement(11572..12369)
                     /locus_tag="PK502_02830"
     CDS             complement(11572..12369)
                     /locus_tag="PK502_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HPG99785.1"
                     /translation="MRTNLLGIIAISGVISLMVSCQPQAEKQEYNQDHMLYSTIWYQH
                     SPEMQALYYQAFNIAGERVRQQSSHKTSKPKAVVVDIDETMLDNSPFQAQEILDNREY
                     STEFWKEWTSLARAKALPGAVEFSKLCDSLKVTLFYITNRSADEAASTLKNLDSLGFA
                     FAKPEYLLPKENESSKKQRRERIAEQYDIILLVGDNLNDFSEIFENRGDDWGSGLVGK
                     NKSDFGSRFIILPNPMYGDWEKNIYHTRGLSPQQKDSLRRSLLNGFR"
     gene            12630..13403
                     /locus_tag="PK502_02835"
     CDS             12630..13403
                     /locus_tag="PK502_02835"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HPG99786.1"
                     /translation="MYPDYHTHTNLSDGRDTHEEMFASAQIRDVSEIGFSDHICVKPV
                     SWAMDVRNIPAMVEAVQMLKQKEGVVRVKQGAEVDFIPGRETETRELLSFIPLDYCIG
                     SVHFIDDWNFDTDILPYDQISINAFYKTYFKLVCQSAKSGLFDIIGHCDLAKKFAYYP
                     TISLDRIFEETARVFSDYGVTVELNTSGKNKPCAEFYPSLRLLEILHHYKVPVTLGSD
                     AHVEQNIGQYFTDAIALLRYVGYKEVSTFSERKRSAIRI"
     gene            13495..15075
                     /gene="nadB"
                     /locus_tag="PK502_02840"
     CDS             13495..15075
                     /gene="nadB"
                     /locus_tag="PK502_02840"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPG99787.1"
                     /translation="MSTFQCDFLVIGSGIGGLSYALKVADYGKVILVTKAGIDDTNTS
                     MAQGGIAAVTYPPDTFEKHVNDTLICGDGYCNEEVVRMVASEAPAQINQLIQWGVKFD
                     RKPNGQYDLAREGGHSEHRILHHKDITGAEIQRALSAGVRQHPNIQVFENHFAVELIT
                     QHHLGRVIHRGDPDITCFGAYVLDQKTQAVHTFLGRSVVVATGGSGNIYHTTTNPRTA
                     TGDGIAMVYRAKGAVENMAFVQFHPTSLYHPGERPSFLISEAMRGYGAVLKTSDGKEF
                     MQKYDPRGSLAPRDITARAIDSEMKQRGDDFVYLDVTFKDPDDTINHFPNIYQKCLSV
                     GIDVTKDMIPVVPAAHYLCGGIKVDMNGQTAIKRLYAIGECSSTGLHGANRLASNSLM
                     EAIVYAERAARHSLATFKSINNSTRIPDWDYEGTTHNEEMVLITQSYKEMQQIMTNYV
                     GIVRSNLRLSRALNRLDIIFEETEELYKKSILTRELCELRNLIQIGYLIIKDAQNMHE
                     SRGLHYSIDYPRQMGTEF"
     gene            15177..16289
                     /locus_tag="PK502_02845"
     CDS             15177..16289
                     /locus_tag="PK502_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPG99788.1"
                     /translation="MPKRKRHYSENPARRIKVGTAIIPVIIGLGVVFLMLFREFNPQA
                     FLQISFTWRSFFWILVAALLMAGRDMGYTFRLMTLSEGKFKFWKAFRVIMLWEFTSAI
                     TPSAVGGTSVAVVYVNKEGLTVGRSSAVVMITSFLDELYFVVMFPILVLLTGPHELFT
                     LASSPAGGMAKDLIIFSIVGYSLKLVWVLGLSYGLFINPRGLKWLIMMIFKLPLLRRW
                     KQGANKAGSDIITSSEEFKKKPFSFWAKAVASSFLSWSSRFLVINALLLAFFDVNHHL
                     LIFTRQLVMWIIMLVSPTPGGSGFAEFVFTRYLSDFIPVGAESLTSIAVGMAMLWRLI
                     SYYPYLFIGAIVFPRWVRKKFGREKGHRSREIATTK"
     gene            complement(16478..17674)
                     /locus_tag="PK502_02850"
     CDS             complement(16478..17674)
                     /locus_tag="PK502_02850"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPG99789.1"
                     /translation="MYGKIQKDLQKQLEAIVNDGLYKTERAISTPQGAEIKANGKKVL
                     NFCANNYLGLSSHPAVLEAGKKAIDEWGFGMSSVRFICGTLELHKQLEAKLTDFLGTE
                     DTILYSSAFDANGGVFEPILGEEDAIISDELNHASIIDGVRLCKAQRFRYKNNDMDDL
                     ERVLKEASGCRYKMIVTDGVFSMDGIIAQVDKICDLADKHDALVMVDDSHATGFVGKT
                     GRGTSEYCNVMGRVDVISTTFGKALGGASGGCISGRKEIVDMLRQRSRPYLFSNSLMP
                     AIAGSTLMVLNMLSKSTELRDKLWENTALFRKGMTDAGFRIVPGVHPITPILLGHLPN
                     DAKLSQDFADMLFDEGIYVTGFFYPVVPKGKARIRVQLSAAHSRENVLFAIDKFTKIG
                     KKLGVI"
     gene            17910..18644
                     /locus_tag="PK502_02855"
     CDS             17910..18644
                     /locus_tag="PK502_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99790.1"
                     /translation="MPYRRLPNTDSARIRALKAAYSKGKLNPPSQLAFSQSTFSKVEL
                     FINSFQNALAHYKVAYNQQVESGKEYSQILKKAKLYLSHFIQVLNMAIARGELPVSVR
                     EIYGMDLDENRMPSLTTEKELIEWGKKIIDGEMQRLQKGQTPITNPTIAVVRVRYEQF
                     LEAYNHHKILQQNTRRLLNDVNNLRVTADSIILSIWNEVEDYFKDLPDEERRLKASEY
                     GVVYVYRKNEIKDFRLEDRTLLKLFN"
     gene            complement(18707..19624)
                     /locus_tag="PK502_02860"
     CDS             complement(18707..19624)
                     /locus_tag="PK502_02860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPG99791.1"
                     /translation="MRAGILSNLSDAKKIVALIILVLLGSFLFTLLGYLVIMAVYGTD
                     SLGWLMNVTISNDTIGALKFLQIVQTIGMFVVPGFTAAWLFSSHPFGYLGFKPSTVQN
                     FITVAVMMVISIPAINLLASINELIPLSGWMVEMERNAEILTEAFLKTDSVGTFLLNL
                     LMIAILPAVGEEIIFRGILQKHLVSATRSRWLGILITAALFSAFHLQFRGFIPRFALG
                     VIFGYLYEWSRSLWLPMLAHFTNNAIAVIAFLLMGSEISVNEIEEVGSLQDIWAIGIV
                     SFLAVALLLRFIWKTNTRQSPVSQNMVKS"
     gene            19878..20906
                     /gene="dusB"
                     /locus_tag="PK502_02865"
     CDS             19878..20906
                     /gene="dusB"
                     /locus_tag="PK502_02865"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPG99792.1"
                     /translation="MRIGEVELGEKPLFLAPMEDVTDPSFRYVCKQFGADVMYTEFIN
                     ADGLIRDAGSSVAKLNIYDYERPIGIQLYGHIIESMVEAARIATACRPDIIDINFGCP
                     VKKIANRGAGAGMLRNINAMVEMTKRIVEATSIPVTVKTRLGWDDESKNIVEIAERLQ
                     DVGIKAITIHGRTRAQLYKGKADWTLIGAVKANPRITIPVIGNGDIDGPVIAGEMFEK
                     HGVDGIMVGRASFGRPWIFREIKHYLSTGQLMEPLTASQKVEIAKLHFQKSLEVKGHK
                     TGILEMRRHFSAYFKGLPNFKEIRLKLLTSTEPEVIEGLLELIRSTYGDFVADGSINP
                     SPWADSAD"
     gene            complement(20935..23706)
                     /gene="polA"
                     /locus_tag="PK502_02870"
     CDS             complement(20935..23706)
                     /gene="polA"
                     /locus_tag="PK502_02870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPG99793.1"
                     /translation="MKKKLLLIDAYALIYRSYYAFIKRPIRTSKGLNTSAIYGFTRAV
                     LDAIRRFKPTHVAVAFDVSGPTFRDEIYPDYKAQRQQTPEDIKISVPIIKQLLDALQI
                     AVVELPGYEADDVVGTLATQANPDEFDVIMLTPDKDYGQLLRKNVSIAKPGRSGNDMQ
                     LVTADEFCRMYDIQNPKQFVDILALWGDVSDNIKGVEKVGEKIAAKLISDFGSIDNIL
                     NNTSKLNDSLRKKIEDSREKLALNKKLVTIVTDLDLKVDIDKDLVSREPNTNELLKLF
                     QELEFRSLIREFDRQPVAAKKEYIQGSLFGEDPEQKVTVIQNLTTIKDIKADYTAVTT
                     AEERKLVVEEISGCTEFAFDTETTGLDPISDSLVGLSVATKPLKAWWIPVPDDRESVS
                     KILAEFKPLFENPQVAKIGQNIKFDMQMLANYGISVKGELYDTMLAHYLLDPDSRHNL
                     NHLSEIYLGYKPIEIEELIGKKGSKQANMRNVPLEKITPYACEDADIALQLKLRLFAE
                     LESQGLTKLYFLLEAPLVEVLAQMERNGVCLDVQSLQEYGKILNSELLKLDAEIKAMA
                     GVPDLNISSPKQLGEVLFDKLRIATDVKMTKTKQYSTSEEELLKYQSAHPIVGKILEF
                     RSIKKLLSTYIETLPGLVNTKTGRIHTSFNQAVAATGRLSSNNPNLQNIPIREENGRE
                     IRRAFIPSPGSYSMLSADYSQIELRLMAHMSGDSAMMEAFRNGEDIHAATAAKIFKVP
                     LEQVSREQRSRAKTANFGMIYGISAFGLSQRLGISRTEAKELIDEYFRTYSGVKRYMD
                     SCIAQARERGWVETLFGRKRYLNDIKSSNQTVRGMAERNAINAPIQGSAADIIKLAMI
                     GIHSEFKRLKLQSKMIIQVHDELVFDVYQPELEQVKEIARSQMEGAAALQIPLVVDIG
                     VGKNWLEAH"
BASE COUNT         6489 a         5419 c         5715 g         6734 t
ORIGIN      
        1 ccaccggggt caataccgag atagggaaaa ttgggaaggc gttggaatca gtgaaagaag
       61 agcccaccag gttgaagagg gagatggcag ttctggtgaa aaggctggca gtaatcggtg
      121 tcatcctgtg cttgctggtg gtgattatat acaccattac ccgtggcgac cttctcaacg
      181 ggtttttggc cggaataacc ctggctatgg ccatgcttcc cgaggaattt ccggttgttt
      241 tggccgtttt tatggcgctt ggtgcctggc gcatatccag gaacaacgta ttgacacgtc
      301 aacctgctgc catcgaaaca cttggctcgg taacagttct ctgcaccgat aaaacaggga
      361 ctcttaccca aaaccggatg acggttaccc agctctacaa tggcactaac catattgctg
      421 taacatcggg cgacacattt accgaaccct ttcatcaggt gattgagtat gccatactct
      481 cgagccaggt gaacccctat gacccgatgg aaagggcaat tatcagcatt ggcgataagt
      541 acttgcagaa ttctgagcac atccacaagg attgggtgat ggagaaggag tacccgctat
      601 caaaggagct gttggccatg tcgcgggtgt tttcaaatac cggaacaggc agaagggtta
      661 ttgcggcaaa aggcgctccg gaggccatat ttgacttatg ccatctccct gctgctgaag
      721 ttggcaagca cgaaaaggcg gtggccgaaa tggcctctag agggctgcga gtacttggag
      781 ttgctcgtgc tgaaatttca acagaaaacc tgcccacaat tcagcatgac ttcaactttg
      841 agtttatggg gctaattggg ctctccgacc caattaggga gaatgttccc gaggcggttg
      901 ccgagtgcta cagagccgga atccgggtga ttatgataac gggcgactat cctgttacgg
      961 ctaccaatat tggtagggaa attggcatca aaaaccctga actttacatc accggtcagg
     1021 atctggaaac catgacggag gaagagcttg gtgagcggat taaacaggtt aacatctttg
     1081 cccgtgttgt tccagaacag aagcttaagc tagtaaatgc ccttaagcgg aatgatgaaa
     1141 ttgttgccat gaccggcgac ggggtcaacg atgcccctgc gctcaaagcc gcccatatcg
     1201 gaattgccat gggggagaaa ggaacagatg ttgcccggga agcctcctcg ctggtgctga
     1261 tggacgataa ttttgcctcg attgttaagg cggtgcggat gggccgaaaa atttctgaca
     1321 accttcaaaa agccctggcg tatatttttg ccatccatgt gcccattgcg gggctatcgc
     1381 ttattccggt tttctttgcc aacctgccac ttattctttg gcctgtgcac atcgtattcc
     1441 tggagctaat cattgacccg gcctgctcca taatatttga agccgaaaag gaggaaaaga
     1501 atgtcatgac acgtcctcca cgtaacatca atcagccctt ctttggtgtc gggaaaatcc
     1561 tgatgagctg cacccaaggg ttgggaattc ttgcagttag cttgctggtt tacttcatag
     1621 gctttcgaat ggggtatgcc gaaaaagccg taagagcatt tacctttatc acccttattg
     1681 tttccaacat cgcagttatc ctatcaaacc gctcatggtc ggattctatt ttcaagatta
     1741 ttatgactcc caacaaaacg gttaagtgga ttgtaggtgg tgctgttctg tttttattgc
     1801 tgattgttaa cattcctgca ctgcaaaacc tcttcctgtt cgataaaatt ggatggcttg
     1861 agttggggat ttgcacgttg gcaggattat caacaattat ctggttcgaa ctctacaagc
     1921 atttcaaaag gataatatag gcacctttcg gggggtatgc cacatggtta ctgctcaatc
     1981 tttttatttg taatatgcta tagtgattga attgtactgt ggcttttatg tgtagccgtt
     2041 agtgcataat ttttaaactt tggcagttgg aggctgaatt taaaggactt tctttgaata
     2101 gtgctgccca accaagggtg cttaccgtat ttgttttagg atggtgctgt ttatcgctat
     2161 actttaaatt tgttttttcc tttgaaacta gtaactttca agccaagccg cttattaaat
     2221 agttaacctg tctttatctt gtgaggggat gccttgaaag ggagtttagc agccatattg
     2281 cttgccggat cgctctcctt ggttacacca aggctatggg gacagccatt gtctttagat
     2341 agccttctcc atgaactttc aaccgtaaaa tccagcgagg gaaagtataa aatccttagc
     2401 agcattgcaa tttcctattc cgatagcagc tacagcaaga gcttggagta ctggttagaa
     2461 gcgctggagg tggcaaaaaa acttgaaagc agtaaatata ctgccgacat cctacatcaa
     2521 ataggattta cctacctaaa aatgggcgag cccaaaagtg cgcttgccaa ctttcaaaat
     2581 gccgctgagc tgtacgtctc tctaaatgac aatgccgatc tggcaggagt ttacaacgat
     2641 atgggcttga tttaccgcag ctggggaagg tacgattcgg cgctggagtt ctatttgaaa
     2701 tcctataagc tttacgaaag gttagccaac gccgaaggga tggctatggc ttccaacagc
     2761 attggccaaa tatactacta ccgcgaaaac tacactaagg cagttgatta cttttccagc
     2821 tacctggaga ttaaccaagg tttaggaaac tatagagccg ttgcgggggc atgcaacaac
     2881 attgccagcg cttacatgga gctcaaccac ctcgatagag cgcttgatta ctacatgaaa
     2941 gcattcaaaa tctatgattc gttgggggta aaaataggtg ttggtatcat acaggataat
     3001 atcggatcgc tcttgtatag taagggagat taccgtaacg cccttcttta ccattttaac
     3061 gctcttcaaa tctttaatga gctgaaaagc gatacgcgta aatgccttac ccttaaaaac
     3121 atcggaagtg tttacctgaa gcaaaaccgc cccgatgttg cggtagccta ccttaaccag
     3181 tcgctggata tggctgttgg aacaggtcaa aaagatgttg cacgagatgt gcaccaaacg
     3241 ctagcaaacg catatgaggt aataggaagc tttcctaaag cctattacca cttaaaaaac
     3301 tatcaggttt taaaggattc catgctaaat gctgaaacca ttgagaaaat cgaaaccctg
     3361 caggcacaac ttgaaatgga aaatcaggca gcgcaggttt ctctggttca gcacaagtta
     3421 aagctacagc ggcaggttgc ctggacggtg agtggcttat tagccatgat ggggggggtg
     3481 cttgttttgc tctacaggga aaacaagaag aaaaacgcag ctcttgccga ggaacaaaaa
     3541 tttaaagaaa gcacagtaaa acggctagat gcatttcttc aaaacacttt aaaggtggcg
     3601 gatgatggca cagtgggagg atttttttcc gatagctgga aattgcaacc agctaacagc
     3661 acccagggta actgcaagtt gctcagcttt aaggtaaata attctaccct tgctttcatg
     3721 caggtaagtg atagccaaag agttagcccc aacctcatta ctctatgcct gtgctgctgg
     3781 gttaaaagca atgcccatcg gctcatgata tcgtgggtaa attttatgaa agagatgagg
     3841 gagaattttg cagctgaccc gcttactagt gatcttgacc cttcacaata ctgtattttg
     3901 ccttttacta ttaatggaga ctttgttttg aaccttacag aggagggttt tgccgtatgg
     3961 catagcaata agctgatatt ccccaaagcg ttccaatgtc cagaacttgt gaagggtgat
     4021 actctttacc tttactttac tacaataggt ttacccttgc cagatagaga gcagcgagaa
     4081 atggaaaaaa taataggcag tgttgcgtct atggaatttt ccctgcaacc ccaggcaatg
     4141 caaaacttgc taaaaacgtt tgagctggaa aattttacct atatatttgc ccttagaatt
     4201 taaaaatttg agaattaatt cttataacaa ctaattacta tggatagcca gtcaaagaag
     4261 accgttaaaa tgctgctaat aagcaactca acaaatgcag gggaagcgtt tctggaacat
     4321 ccaaaggaag agcttaaaag gtttctgaat ggcactaaaa gggtgctttt cattccgtgg
     4381 gcagccgtaa cttttagcta cgatgagttt gaacgtaagg tacaagaacg cttcagcgag
     4441 tttggcgttg aggttgactc cattcaccac tatcgaaatg ttaaaacggc ggttcaggag
     4501 gctgaagcca ttgttgttgg tggtggtaac acctttaagc tgcttaagac tatacagcag
     4561 catgatatag ttgaaactgt tcggaataag gttttggatg gcacccccta cataggctgg
     4621 agtgctggag ccaacgtggc ctgccccacc atttgcacaa ccaacgatat gcccatcacc
     4681 gaacccgata actttggtgc ctttagcctg attcgctttc agattaaccc ccactacctg
     4741 gatgcaaacc ccgcaggaca tgctggcgaa acccgggagc agcgcattat ggagttcctc
     4801 gaaatggatc catacgttta cgtggttggt ttgcgggagg gttcgatgat tctgctggag
     4861 aatgttgctc tgaccctaaa aggaccaaaa tctgccagaa tattcaaaaa aggtattccc
     4921 ccatttgagg ttaacccggg tgatgacatg gattttctat tcgaatagca ctaccagccc
     4981 tgttgagaaa agcaatacgg gggaaatagt tcctacacgg ctcgtgctac ataatcctta
     5041 ggaagggtta ccagcaggga gtggcttaca tgatcgatac ggataataac cttctttttt
     5101 ccggcatgtt caaccagctc accggtaatc cccatcaggt taccaaatct aacttcaacc
     5161 aaggctcctg gtggaatttg ctcttcaacc ggttcaattt ccaaatcctg aaccagcagc
     5221 tgttttacct gctcaatctg cctgtcggga atgggaacgg cctttccctc aaaggtgatg
     5281 tacctaacaa ttcctggagt gttcaacacc tcgtagtact cccgggcatc aatccgaaca
     5341 aaaatgtatg accggaacaa aggctcctcc accaactttt tcctatcgct ccactgcttt
     5401 agccttttct gtaagggcag aaaggcttcc acgccttttt ccaccaaccg gctatatatt
     5461 tttttttcat tccgcggttt agtgtacagg gcataccaac gcttattggc ttcaccttta
     5521 tccatctgtt gggattttta gcaaaattac tacttttggt tttatacgtt caggtagcat
     5581 tttaaaaata ccgcgttctg tgacttgctc gagtgaggaa gacctgcaat aaaccccact
     5641 tcaaattctc cctttttgta gggcatattt gttcgattat tgtccaacct aaggcataag
     5701 gtaaaatcat ctggcgtact ggtaaaacta aaatttcata atttcgttta agtgataaaa
     5761 aacgattttt ttaccgccac caatgaacgt gtctaaaaaa aactaatttt tgtcgaaaag
     5821 gctaagcttc tattaagaaa attacctaaa cttgcgttta atcactttaa ctataatcta
     5881 ttaatcagca aattaaaact aaatgagaat taacttttaa cagatctcat gtgatgaagt
     5941 caaagaaaga ttttcggaaa cccgtaaaga atgactccta cgattccgat gaggtgaaaa
     6001 tggttaaaaa gctaaaaccc gtgaaaaagg aaaaaaatgc taagcgttca atcttcagtg
     6061 aaattgatga gcttgatgac ctggtagatt tcaaggggac agtgttggac gaaaaatttg
     6121 aagactatga cgaagaggag gaagagctgt agtaggtaat actcagggct atagataatc
     6181 aagtaaggct gccggaaggg cagccttttt tatttttctt ttacttttcg ttgtgcacaa
     6241 cgctcttatc cttaacatag aagggtcttt caattgcctg gtcgagcgag ataaaagttt
     6301 cggttcggga tatgccgggt atattctgaa tggtgttaac cagcacgtcc atcaggtgct
     6361 cgttatccct acagtagatt ttgagcagca aagcatactc tcctgttata aaatggcatt
     6421 ctaccacctc gggcattagc ttcagggctt caatgacgcc gctgtacagg ttggtggaaa
     6481 caagctgcac gcctacaaat gcacatacgt caaaccctaa ggctttgggc ttaacaatca
     6541 ttcgcgatcc ggcaattatt ccggcatctt ccatcttttt tactcgctga tgtatggctg
     6601 ctcccgatat cccacactcc cttgcaatct caagaaatgg tatcctggcg ttttttacca
     6661 ggtacgaaag tatcttctga tcgattacat ctaccctaaa tttttcctgc atggttgttt
     6721 tgaattgata cgttttgggt gataaaatta aaaatgtatt gttaaaaaga caaaataaat
     6781 tataaaaatt aaccttatct gattaatttt tttgcaatga tttcctaaaa cgcttacata
     6841 ttcagggagt ttgttgtgtt gcaaaaaaaa cggtacttaa tttcgaacgg taattggcag
     6901 tattaaaaca agttactact attttaaatg atgacatgaa ggacaattga tgacatggca
     6961 actcctttct ccatgtcggg tttgagaaaa aatgaaggtt gatttttagt agatgcatct
     7021 ctctgattca tttcctgact ggtttgcttt gcaaagataa aaagtcaaaa agggcttaga
     7081 atatgctggt ttatgtttaa aggctttttg aatatacaac cttatcgtcg ccttcgtcat
     7141 aaaagttttt aagtattgcc tcgttctcgt aggcattgct aaggtaaaat tgccgggtgg
     7201 gctcgtacat tggttttgat gaggtttcaa tgtatatttt tcgcccattc agctgtttaa
     7261 tctcattttc taccctttgc agtacctgtt tgccaaggcc tttccccctg taatcctggt
     7321 gggttccaat ccagtacaga tcgaagctgt ggcgtgtgca aggaattgga ccaaaacagg
     7381 cgtaggatat cgtagtgccc atgtggtcgg caaaaacaaa gtagtaaccg ctttccgttc
     7441 cctttgccaa agcctcctcc accagctcta tggctacatc aatttcaaaa tcgtgaaaaa
     7501 atccagatga ggatagtatt tctctgatac cggctttgtc ggcgggcgtt ggggtggagc
     7561 gaaaagttac attgtccata aggctgaacg ttatgttaaa tttgaaaggg aatatcggcg
     7621 ataatgctcc ttaccgcttc atcaaacgga attcctgcct gcatacaagc cgcatagaat
     7681 ccgctatctc tcgaaataca agggttgacg ttgatttcca gcacccacgg gtttcctgcg
     7741 ttatcaaccc gaaaatctac cctggcgtag ccattcaccc taaaggtttt ccagcagtga
     7801 aggcaaatat cacccatggt gctgagcaac ggttggtcat ttggagacag gtcaaaagtt
     7861 ctgtttgtgt tgatatactc aaagctatcc tcaatccact tggcggagta gccaacaaca
     7921 tgcggcttgt cgggagcata gttacgaaat atgatttcgg ctggcggtaa tagttgtgga
     7981 ccgttttgtc cggccaaaat agatatgtta aactcgcggc cgtccacaaa ctcctctata
     8041 aaatgggtgt tgtagtcaat gctatttaaa atttcggcta gtttcgggtt gcttccctca
     8101 aaaattgaat cctcatcgag gcccagcgat ccatcctccc agattggttt tacaatgtaa
     8161 cgtttatccc gatcgggaat aaatgaaccg tttccctggt accaaaaggg cgttgggatg
     8221 cctgcatcct gcatcagctg cttggttagc gttttggatg atgtgaggaa tatggtttcg
     8281 agcgagccgc cggtgaatgg aattcccata gagttaagca gggaaggggc aaggaaaatc
     8341 aggtttcccc tgttgttcac cgattcaacc aggttgaaaa caaagtcggg ctttagcctg
     8401 ctgagctcat cacgaacctt ttgaaggtta agggaaaatt ccgcgcgctg gctctcaata
     8461 cccagcttgt tgagcatctc ggcaacaagg gcaacctggt ccagaacatc cctttcgtcg
     8521 ggaccggcgc tctccgaaag ctcgttgtat aggattaccg catgtattgt ttgcctgttc
     8581 atgccgtatg attaaaccgt tccattgccg aatccattat cagctgaata agcatggtaa
     8641 aatcaatgcc atttttttga gcgagcatgg ggaaatcaga gtggaatgga ttcaggcctg
     8701 caagaggatt aacctcaatg aagttgggtt tgcccgaagc atccattttt aagtcaaccc
     8761 ttccaccatc gcgacaaccc aggcaattcc atgccattag agcagtttcg gcacaaagcg
     8821 attctatttc gccggttgcc atgtagtagt ccacaatgcc cttccaattc tccttggtgt
     8881 gtagggagta ggtaccatct tttgctctgt tggaaatcac tatctccata acccctaggc
     8941 agcgtgcctt atcgcctgta ccaactatcc caacggtgaa ttcacgcccg ggaaggtagg
     9001 tctcgacaag aataggttga cgatattctt cgagcaaccg tgctgtggtt gcatagagca
     9061 cttcttttga gtttatcttc gactggccat taatcccttt tcctgttccc tctgcagctg
     9121 gctttacaaa cagggggaag ggcagagcta ttttctcaac ctcgcttagg cttgtaataa
     9181 cggcgaaatc ggctgtaggc acgccgcagt cgcgaaccac acgctttgcc tgccccttgt
     9241 gtagggtaag tgccagcacc agggggtcgg aaaacacgta gggaatttga aacgcatcca
     9301 gaatggcagg aacctgtgct tctcttccaa ttccgtgcag cccttcggca atattaaaaa
     9361 ccatgtccca acgtttgccg ttggccagcg catgggtgag gttgatgatg tttccaaccc
     9421 gctcggtgaa gtagccaaga gactgcagtg tactctcaat gctgtcaatt gtttcgtcag
     9481 agtcaaactc ggccacttcc tcatgtgagt agccattttt caggtagtcg cttttcaggt
     9541 cgtatgttaa gccaactgtc atctattttt gtgtttatca gcgtgagcat taaaaactct
     9601 tgggaaagcc atttgagcgc atcaagttta tttttttgaa aaaggtgtgc agtaaatagc
     9661 gttatggtaa aaaccacctg tccgaagtaa gtgaaagcaa agggacaggt ggttgaacta
     9721 tacaattgcc attgttttaa tgtaaatcag ggtagtaaaa ggttttcccc tcgtagtttt
     9781 taagcacaac gtatccctcc tcatttctcc cctggtagta ttcaggcaga aggggaattt
     9841 ttccaccgcc accaggtgcg tcaataacaa agtggggaat ggcgtatccg gtggtccagc
     9901 ccctaagtcc tttaattatc tcgaggccct tttcaacaga tgttctaaag tgggaagagc
     9961 cgggtatggg atcgcactgg taaaggtagt aaggtcggac cctaattttt agcagtcctt
    10021 gaaataggga cttcatggtg tccacattgt cgttaatacc tttaagaaga acagtttggc
    10081 taccaagcgg aataccagca tcggcaagcc gcctgcatgc ctcggctgtt tccggggtaa
    10141 gctcatcggg atgggtaaaa tggatgctga tgtagagggg atgaaatttc cggagaattt
    10201 ttatgagctt aggagtaatt ctatggggaa gaacgaccgg aaccttggtg ccaatccgta
    10261 tcatttcaac atgtggaatg cttctaagct gggaaaggag gtactcgatg tgtgcatcgg
    10321 acatggtaag cgggtcgcca cccgagatga ggacgtccct aacctcggtg tgctccctaa
    10381 tatagttaat tgctggtagc caggcgttga ccccaacatg gcacttatcc ttggccacca
    10441 tgtgcgaacg ggtgcagtaa cgacagtagg ccgagcagaa tccggttgaa agaaacagca
    10501 ccctgtccgg gtagcgatgt accaggtttg gaacggggct ggaatgtccc tcgtttaatg
    10561 ggtcgcacga ctcacctgcc gaggtggtaa gctcgttaac ggtgggtatc atggtccgac
    10621 gtagcgggtc ctgggagttt ttcccattca gcaggctggc gtagtagggt gttattcgta
    10681 atggcagcga atgtttgggg tggtcaattg cctcgcactc ctccctggaa agatcaagga
    10741 agcatttgag tttttcggaa gttgtgaagc tgtttttcag ctgccatttc cagctcatcc
    10801 agtcttccac cgtggcgttg gggaagtact ttatcaagaa ttccagcgaa cgtttgctga
    10861 ttggaaatgg ccaattgcag gagggtgatt tgtgttgatg ggaagttgcg gtaattaaac
    10921 cgtaatttgg gcttagctgc cccgaacccg gaggttcatc atcttcttct aagcctagcg
    10981 tgttaagcgt ttcatgcatt agttctttgc tcatgacttt aaaatttagc gtcgcaaatg
    11041 ttgttattta ttcaatacct tgttcaggct tcttaatttt tttagctaaa aaatcattaa
    11101 tgccggtgtt tttaaaaatt tcgacgaaat taagttgcta tcagcatttt tattcatggg
    11161 gaaggcgata gtaattaaca gtttgcatga gttatcaaca ggcgtaaaga tgcaacccct
    11221 tggattattg gttaacatat aaaaatattc ctatctttgc agctggttat ggccttatag
    11281 ttcaagggat agaacggaag tttcctaaac tttaaatcca ggttcgagtc ctggtaaggc
    11341 cgccaaccta acaacaagcc tttaagctca taaaggcttt ttttattacc tccggattgt
    11401 ggagacacaa acttaaagac tatactgttg ttagtcgcat tacacactaa cagcaacatt
    11461 ttcatgctaa agaatttgca gcatggctaa ttttatatac agcactccag cagccacaca
    11521 gaatgatgca aatatcctac tagctagttg ggtagaaaca aaggttgaat gttacctgaa
    11581 tccatttaaa agcgagcgac gcaagctgtc cttttgctga ggagaaagcc cccttgtatg
    11641 gtaaatgttt ttttcccaat cgccatacat tgggtttgga aggatgatga accggctgcc
    11701 aaaatcactt ttattcttgc ctaccaaccc agacccccaa tcgtcgccgc gattctcaaa
    11761 tatttcggag aaatcgttga ggttgtcgcc aaccagcagt ataatatcgt actgttccgc
    11821 aatcctttca cggcgctgct tcttactgct ttcgttctcc tttggcagaa ggtactcggg
    11881 tttggcaaat gcaaaaccta acgagtctag attttttagg gttgatgctg cctcgtcagc
    11941 agagcggttg gttatgtaga aaagggtcac tttgagcgaa tcgcaaagtt tgctgaactc
    12001 aacggcaccc ggaagggctt ttgcccgtgc aagggatgtc cattccttcc agaattcagt
    12061 ggagtattcc ctattgtcta atatttcctg tgcctgaaat gggctgttgt ccagcattgt
    12121 ttcatcaata tcaaccacca ctgcttttgg ctttgaggtc ttgtgcgacg attgctgtct
    12181 aacacgttcg ccggctatgt tgaaagcctg gtagtatagc gcctgcattt cgggtgaatg
    12241 ctggtaccaa attgtggagt acagcatgtg atcctggttg tactcctgct tttcagcctg
    12301 gggttggcac gacaccatca aggatattac gcctgaaata gcgattatcc ctagtagatt
    12361 tgttctcatg gtttaccttg ataatttaat ggttatgcgg tagcgaacta cttaataatt
    12421 aaggttatgc taactaaatg ggctttccat ctaatcgttc aaactttaac atatttgcaa
    12481 aggaatcaca gctggcttta gagcagttgc agcctgtaaa gttaagcgtt ttaccagtag
    12541 tggattttgc atggaaaaat tttggtaaaa aattgcatac ttttggtagt tggcaacatt
    12601 ttttacccat aattgttgaa atttatatca tgtatcccga ttaccacact cacaccaacc
    12661 tatccgatgg gcgcgacact cacgaggaaa tgttcgcttc ggcccagatt agagatgttt
    12721 ctgaaattgg attttccgat cacatctgcg taaaacccgt aagctgggca atggatgtca
    12781 gaaatattcc tgctatggtt gaagcggttc aaatgcttaa gcaaaaagaa ggagtggtta
    12841 gggttaaaca gggtgcagag gtcgatttca tacccgggcg ggaaaccgaa actcgggagc
    12901 tgctcagctt tattccgctt gactactgca ttggttctgt tcattttata gacgattgga
    12961 atttcgatac cgatattctg ccctacgatc agatcagcat aaacgctttt tataaaacct
    13021 acttcaagct ggtttgccag tcggcaaaat cgggactttt cgatattatt ggccactgcg
    13081 acctggccaa gaaatttgcc tattacccca ccatcagcct tgaccgaatc tttgaggaaa
    13141 cggcaagagt cttttcggat tacggggtaa ccgttgagct gaacaccagc gggaaaaaca
    13201 agccctgtgc cgagttttac ccctcccttc ggctactgga aattctgcat cactacaagg
    13261 tgccggttac gctgggctct gatgcccatg tggagcaaaa catagggcag tactttaccg
    13321 atgccattgc cctgctacgg tatgtgggat ataaggaagt ttcaaccttc tccgagcgta
    13381 agcgaagcgc cattcgaatt taactaacta tgacacacta gtcggtttat ttgtattttt
    13441 acccgttttt ttatcaatca ttcagtacta caggttagat gaaatttaac taccatgtca
    13501 acctttcagt gtgatttttt agttattggc tcaggaattg ggggcttaag ctatgcccta
    13561 aaagttgcag attatggcaa ggtaattctg gtaactaaag cggggataga cgataccaac
    13621 acctccatgg ctcaaggtgg aattgcagca gttacctacc ctcccgacac ttttgagaag
    13681 catgtaaacg ataccttgat atgcggcgat ggatactgca acgaggaggt ggttcgcatg
    13741 gttgcctcag aggcccccgc ccagataaac cagctaatcc agtggggcgt aaaatttgac
    13801 agaaagccta acgggcagta tgatttagcc cgcgagggag gtcattcgga gcaccgaata
    13861 cttcatcaca aggatataac cggcgctgaa attcagaggg cgctgagtgc tggagttcgt
    13921 cagcatccca acatacaggt ttttgaaaat cactttgccg ttgaactcat aacccagcac
    13981 catttggggc gggttattca ccggggcgat cccgacatta cctgttttgg agcctatgtg
    14041 ctggatcaaa aaactcaagc ggttcataca tttttaggca ggtcagtggt ggtggcaact
    14101 ggcggttctg gcaatatcta ccacacaacc actaatccca gaactgcaac cggcgatgga
    14161 attgccatgg tttacagggc aaagggcgcc gttgaaaata tggctttcgt gcagtttcac
    14221 cccacatccc tctaccaccc gggggaacgt ccgtcttttc tcatatcgga agctatgcga
    14281 ggctacggtg cagtgctcaa aacatccgac ggtaaggagt tcatgcagaa gtacgaccct
    14341 cgcggttctc ttgctccccg cgatattact gcccgggcca tagattcaga aatgaagcag
    14401 cgtggcgatg acttcgttta cctcgacgtt acctttaagg atccagatga caccatcaac
    14461 cacttcccca acatttacca gaaatgccta tcggttggta ttgatgttac caaggatatg
    14521 atacctgttg tgcctgcagc ccactacctt tgcggtggaa ttaaggttga catgaatggt
    14581 caaacagcca ttaaaaggct ttatgccatt ggcgaatgct cgtcaaccgg gctgcatggg
    14641 gctaaccgac ttgcttccaa ctcgctgatg gaagctattg tttatgccga aagggctgcc
    14701 cggcattcac tggcaacttt taagagtatt aacaacagca ccagaatccc cgactgggat
    14761 tacgagggaa ccacccacaa cgaggaaatg gtgcttatca ctcaaagtta caaggaaatg
    14821 cagcaaatta tgaccaacta tgtgggtatt gttcgttcta accttcggct ttcgagagct
    14881 cttaaccgct tagacatcat atttgaagaa accgaggaac tttacaaaaa atccatactc
    14941 accagggagt tatgcgagct gcgcaacctc attcaaattg gctacctaat tattaaggat
    15001 gcccaaaata tgcacgaaag cagggggctt cactactcaa ttgactaccc acgccagatg
    15061 ggtacagagt tttagcagga agcaacaaca cctttattga aatggagtgg tagttaaaag
    15121 tttgttatga ttgtgaatag cgttagcacc aagtagcctt tacctccaga ctgctcatgc
    15181 cgaaaagaaa acgccactat tctgaaaatc cagcccgacg cataaaggtt ggaactgcta
    15241 tcatccccgt aataattggt ctgggggtgg tttttttaat gcttttcagg gagtttaacc
    15301 ctcaagcctt tttgcagata agctttacct ggcgttcatt cttttggata cttgtagccg
    15361 cacttttgat ggcaggaagg gatatgggct ataccttcag gcttatgacc ctttccgaag
    15421 ggaagttcaa gttctggaaa gccttccggg taataatgct atgggagttt acctcggcca
    15481 ttacgccttc tgctgttggc ggaaccagcg tagctgttgt ctacgtcaat aaggaaggtc
    15541 ttacggtggg tcgcagctcg gcagttgtta tgataacctc ctttctggac gagctatatt
    15601 ttgtagtaat gttcccgatt cttgttcttt taaccggccc tcacgaactt tttacccttg
    15661 cttcttctcc cgctggtggt atggcgaaag accttataat tttcagcata gttggctatt
    15721 cgttaaagtt ggtttgggtt ttaggcttat cgtatggcct ttttataaac ccacgggggc
    15781 tgaaatggct tatcatgatg atttttaagc tgccgttgct gcgcaggtgg aaacagggtg
    15841 caaacaaggc cggaagcgat ataataacga gctcggagga gttcaagaaa aagccattct
    15901 ctttttgggc taaagccgtt gcttcttcat tcctttcatg gtcgtcgagg tttttggtga
    15961 taaacgcact tctacttgca ttttttgatg taaaccacca cttgctaatt tttacccgac
    16021 agctggttat gtggattatc atgctggtaa gccctacgcc tgggggaagc ggttttgccg
    16081 aatttgtatt cacccgctac ctgtcagatt ttattcccgt aggagcagaa tcgctcactt
    16141 ccattgctgt tggaatggcc atgctctggc ggttaataag ctactacccc taccttttca
    16201 ttggtgcaat agtttttccg cgctgggtaa gaaaaaagtt tgggagggaa aaaggccaca
    16261 gaagccgaga aattgcaacc acaaaataat tagggttgat ttttgtggaa ttcataccct
    16321 aggttcgtct tgttaagtca gcattctggt taggcagcac aacataacga gtcataccca
    16381 tttcacgttg aatcatacct acgctgtgct gtaataaaaa agacctgctg ttagtttagc
    16441 aggtccgttc ttattttgat tggcattgtg aacagggcta tataacaccg agtttttttc
    16501 caattttagt aaacttatca atggcgaata ggacattttc cctactgtgg gcagcactaa
    16561 gctgaacacg tatgcgggcc tttccctttg gaacaaccgg ataaaagaac cccgtaacgt
    16621 aaatgccctc gtcaaaaagc atatcggcaa aatcctgcga caacttggca tcgttgggta
    16681 ggtgtccaag caggatgggg gtaatggggt gaacaccagg tacgattcta aaaccggcat
    16741 cggtcatacc ttttcggaaa agggcggtgt tctcccaaag cttatcgcgc agctcggtgc
    16801 ttttgctgag catgttaaga accatgagtg ttgatccggc aatggccggc attagcgagt
    16861 tggagaaaag gtagggtctg gagcgctggc gaagcatgtc aacaatttcc ttccgtcccg
    16921 aaatgcaacc gcccgaagct cctccaagag ccttaccaaa agtggtagag atgacgtcaa
    16981 ccctgcccat tacgttgcag tactcggaag ttccccttcc ggttttgcca acaaatcctg
    17041 tggcatggga gtcgtcaacc ataaccagcg catcgtgctt atcggcaagg tcgcaaattt
    17101 tatctacctg ggcaattatg ccgtccatgg aaaaaacgcc atcggttacg atcattttat
    17161 agcggcaacc ggaggcctcc ttcagcacgc gctccagatc gtccatgtcg ttgttcttat
    17221 accggaagcg ctgagccttg cacagcctta ccccgtcaat aatggaggcg tggttgagct
    17281 cgtcggagat aatggcatcc tcttccccca aaatgggttc aaaaacccct ccgttggcat
    17341 cgaaagccga agagtaaaga atggtatcct cggtgccgag gaagtcggta agcttggctt
    17401 cgagctgctt gtgcagctca agggtgccgc atataaaccg aaccgacgac atgccaaaac
    17461 cccattcgtc tatggctttc tttccggctt ccagcacagc aggatgcgat gaaagcccaa
    17521 ggtagttgtt ggcacaaaag ttcaaaactt ttttcccgtt agccttaatt tcagcgccct
    17581 gtggtgtaga gattgcacgc tcggttttgt agaggccgtc gtttacaata gcctcgagct
    17641 gcttctgtaa atctttctga attttaccgt acatggtata atcattttaa attctcttta
    17701 caaaagtaga agaattttat gcggggcgag ctatgaaatc gcctttcttt tcttcatttc
    17761 tcaaaaaaat atctactaag caaaatattt gccatgcaat ttaaaaatta tttaatgcga
    17821 atagatggct tttgtaatca tgatttttta tattcgcaaa ttgtaggtag ttaattctca
    17881 gagcgaaaaa cccaacaacc gattaaatta tgccctaccg ccggttaccc aacacagata
    17941 gcgcaaggat acgcgcttta aaggctgcat acagcaaggg aaaattaaat cctccatcgc
    18001 agctggcctt ttcgcagtca accttcagca aggttgagct attcattaac tctttccaga
    18061 atgcgctggc tcattacaag gtggcttaca accagcaggt ggagagcggc aaggagtact
    18121 ctcaaatttt gaaaaaggca aagctttacc tttcgcactt catacaggtt ttaaacatgg
    18181 caattgcccg aggcgagctt cctgtttcgg tgagggagat ttacgggatg gatttggacg
    18241 aaaacagaat gccgagcctg acaaccgaga aggagctgat cgaatggggt aaaaagatta
    18301 ttgacggcga gatgcagagg ttgcaaaagg gacagacacc aatcaccaac ccaaccattg
    18361 cggttgtgcg agttcgctac gagcagttcc tagaggctta caaccaccat aaaatattgc
    18421 agcagaacac gcgcaggctg cttaacgatg ttaacaacct tagggttact gccgatagca
    18481 tcatcctaag tatttggaac gaggtggaag actactttaa ggatttaccc gacgaagaac
    18541 gccggttgaa agcttcggag tatggggtag tttacgttta ccgcaagaac gagataaagg
    18601 atttccggtt agaagacagg acgctgctaa agctattcaa ctaacgcgca ttcaccttca
    18661 cactgatcca catggacgct accgggaagc tgcatgtttt tagcagttag ctttttacca
    18721 tgttttgact taccggcgac tgccgggtgt tggttttcca tataaatcga agcagcaacg
    18781 ctactgccag gaagctaaca attccaatag cccaaatgtc ctgaaggcta ccaacctctt
    18841 ctatttcgtt tacgctgatt tccgaaccca taagcaggaa ggcaattacc gcaattgcat
    18901 tgttggtgaa atgggccagc atggggagcc acaggctgcg actccactca tacaggtagc
    18961 caaaaatgac acccagcgca aaacggggaa tgaaccctct aaattggagg tggaatgcgc
    19021 tgaatagcgc tgctgttatc agtatcccga gccacctgct gcgggtagct gataccaggt
    19081 gtttttgcaa aattcctcga aaaattatct cctccccaac ggcaggaaga atggcaatca
    19141 tcagcaggtt tagcagaaac gttccaacgc tgtcggtttt cagaaatgcc tcagttagaa
    19201 tctcggcgtt tctctccatt tctaccatcc agcccgatag gggtatcagc tcgttaatgg
    19261 aggccagcag gttgatggcc ggtattgaga taaccatcat tacggctaca gttataaaat
    19321 tctgaacggt ggatggttta aaacccaggt agccaaatgg atgactggaa aatagccatg
    19381 ctgctgtgaa tccgggcaca acgaacatgc ctattgtttg tacaatttgc aaaaacttta
    19441 acgcaccaat tgtatcgttc gatattgtta cgttcattag ccaaccaagc gaatcggttc
    19501 catacactgc cattataacc aggtagccta gtagggtaaa aagaaacgac cctaatagaa
    19561 caaggataat cagcgcaaca atttttttag catcagaaag attcgataga attccggctc
    19621 tcatacgatg gttgttagaa ttagtgcagg gaaaaaatgg ataccatagc gaaaaaaaac
    19681 tcaagcaaaa cccaactggt taaagtgcag ttgcaaaggt agaagttttc ggtaaactgt
    19741 ggatttactt gtcaaaatgc cagataatca gaagccgata aaatctattt ttactcattt
    19801 gcagaccagc gctttcggct cgttggttat gcagagggaa aaacattttt tacttttgca
    19861 gctcaactgc atgacaaatg cgtattggcg aagtagagct aggcgaaaag cctctttttt
    19921 tagcaccaat ggaggatgtt accgatccgt cgtttcggta tgtatgcaag cagttcggcg
    19981 ctgatgtgat gtacaccgag tttattaatg ccgatgggct tatacgtgac gccggcagca
    20041 gcgtggcaaa gttgaatatt tacgactacg aaagaccaat aggcatccag ctttacggtc
    20101 atattattga aagtatggta gaggccgcaa ggattgctac agcctgtcgt cccgatatta
    20161 tcgacattaa ctttggctgt ccggttaaaa aaatagccaa tcggggtgct ggagccggaa
    20221 tgcttaggaa tatcaatgcc atggttgaga tgaccaaacg aattgtggag gccactagca
    20281 ttccagttac cgtgaagacc cgtctagggt gggacgacga atcaaaaaac attgttgaga
    20341 ttgccgagcg gctgcaggat gttggaatca aggctataac cattcatggg cgcacccgag
    20401 ctcagctcta caaaggcaaa gccgattgga ctcttattgg tgcggtgaaa gccaaccccc
    20461 gaataaccat tccggtgata ggtaacggcg atattgacgg tccggtgata gccggtgaga
    20521 tgttcgaaaa acacggtgta gatggcatca tggttggcag ggcatcattt ggaaggccat
    20581 ggatattcag ggagattaag cactaccttt ctaccggcca actcatggag ccccttacag
    20641 ccagccaaaa agtggaaatt gccaagctgc attttcaaaa atcgctcgag gtaaaaggtc
    20701 ataaaaccgg aatcctcgaa atgcggcgtc atttttcagc ctacttcaag ggacttccca
    20761 attttaaaga aatcaggcta aagctgctta cctctaccga gccagaggtg atagaaggct
    20821 tacttgaact gatcaggtca acttatggtg attttgtggc agatggcagt ataaacccct
    20881 caccctgggc agattcggcc gattagacat ttttctccgt ttcgagaaat gcagctagtg
    20941 ggcctccagc caatttttgc ctaccccaat atccaccacc aggggaattt gtaaggcggc
    21001 agcaccctcc atttgcgacc tggcaatttc cttaacctgc tccagctccg gttggtaaac
    21061 gtcaaacacc agttcgtcgt gaacctgtat aatcattttc gactgcagct tcaaccgttt
    21121 aaactcggag tgaatgccaa tcatggccag cttgattata tcggctgcag aaccctgtat
    21181 tggggcgtta atagcgttac gttcggccat tccgcgaact gtctggttgc tcgatttaat
    21241 gtcgtttaga tagcgcttgc ggccaaaaag ggtttcaacc cagccccgtt cgcgtgcctg
    21301 ggcaatgcag ctgtccatgt agcgcttaac cccggagtag gttcgaaagt actcgtcaat
    21361 cagctccttt gcttcggtgc gcgagatgcc gagccgctgg gagagaccaa aggccgagat
    21421 gccgtaaatc attccgaagt tggctgtttt tgcccggctc cgttgctcac ggctcacctg
    21481 ttcaagggga accttgaata ttttggcagc cgttgcggca tgaatatcct cgccgtttcg
    21541 aaaagcctcc atcatggccg aatcgccgct catgtgggcc ataagcctaa gctcaatttg
    21601 ggaatagtcg gccgagagca tggaatagct gcccggtgat gggatgaagg ctctccgtat
    21661 ctcacggcca ttctcctcgc gtatgggtat gttttgcagg ttggggttgt tggagcttag
    21721 gcgtccggtt gcggcaaccg cctggttgaa cgaggtatga attctcccgg ttttcgtgtt
    21781 caccaacccg ggaagcgttt cgatgtaagt ggacaggagc tttttgatgg agcgaaactc
    21841 cagtattttt ccgacaattg gatgggcgct ctggtacttt agcagctcct cctcgctggt
    21901 ggagtactgc ttggtcttgg tcattttcac atcagtagca atccgaagtt tgtcgaaaag
    21961 cacctcgcct agctgtttgg gcgaggagat gttgaggtcg gggacacccg ccatggcctt
    22021 aatttcggca tccagcttaa gcaattcgga gttaagaatc tttccatact cctgtaggct
    22081 ttgcacgtct aggcaaacac cattgcgctc catttgagca agcacttcaa ccaatggtgc
    22141 ctcgagtagg aagtagagtt tcgtaagacc ctgcgactca agttcggcga aaagcctcaa
    22201 tttcagctgt agggcaatgt cggcatcctc gcaggcgtat ggggtgattt tctccagggg
    22261 gacgtttcgc atgtttgcct gctttgagcc ttttttcccg ataagctcct caatttcaat
    22321 aggcttgtaa ccgaggtaga tttccgacag gtggttcagg ttatgccgcg agtcaggatc
    22381 gagcaggtag tgggccagca tggtgtcgta gagttctccc ttgacggaaa ttccatagtt
    22441 ggccagcatt tgcatgtcaa attttatatt ctgcccaatt tttgccactt ggggattttc
    22501 aaacaggggt ttgaactcgg ctaaaatttt gctaaccgac tcacgatcgt caggaacggg
    22561 tatccaccat gcctttaggg gttttgtggc aaccgacagg ccaaccaggc tatcggagat
    22621 aggatctaac ccggttgttt cggtgtcgaa ggcaaattcg gtacaccccg aaatttcctc
    22681 taccacaagc ttcctctcct ctgctgttgt aacggctgtg taatctgcct ttatatcttt
    22741 tatggttgta aggttttgga ttacggtaac cttttgctct ggatcttccc caaacagact
    22801 accctgaata tactcctttt ttgcggctac cggttgccgg tcgaattccc tgattagcga
    22861 cctgaattcc agttcctgaa atagcttcag gagttcgttg gtgttgggtt ccctgctcac
    22921 caagtccttg tcaatatcaa cctttaggtc gagatcggta acaatggtaa ccagcttctt
    22981 gttgagtgca agtttttcac gtgaatcttc aatttttttg cgtagcgagt cgtttagctt
    23041 tgaggtgtta ttcagaatgt tatcaatgct gccaaagtcg gaaatgagtt tggcggcaat
    23101 tttttcgcca actttttcaa ctcccttgat gttatcggag acatcgcccc agagcgccag
    23161 aatatcaaca aactgtttgg gattttgaat gtcgtacatg cggcagaact catcggcggt
    23221 tacaagctgc atatcatttc cgcttcggcc gggcttggca atagaaacat ttttacgaag
    23281 cagctggcca taatccttgt cgggtgtcag cataatcacg tcaaactcat cgggattggc
    23341 ctgggtggca agggtgccaa ccacatcgtc tgcctcatag cccggcaact caactaccgc
    23401 tatttgcaag gcgtccagca gctgctttat gatggggact gaaatcttta tatcctctgg
    23461 tgtttgctgt cgttgggcct tatagtcagg atatatttcg tcccgaaagg tgggtcccga
    23521 tacgtcaaaa gctacggcaa catgcgttgg cttaaatctt ctgatggcat caagcacggc
    23581 tcgggtaaac ccgtaaatgg cggaggtgtt aaggcctttg ctggttctaa ttggtcgctt
    23641 aataaaagcg tagtatgacc ggtaaatcag tgcgtaggca tcaattagca ggagtttttt
    23701 tttcatccgt attggtgtat tcttactggg ttttagttgt aagtgctacc ctttttcact
    23761 tgatgtgctt aggccgaagc ggctaaccca agagcaaagg tacacctttt aggtgatgac
    23821 gcaacaggag tttctgaaaa aatgcgaggt ttgaattgaa cagcggggtg gtcaaaacga
    23881 cagaaaccgc agtgaaatgg tcatttttat gctcgtatat tggaatagcg ttttcgagtc
    23941 tcataataat tcaaaagcat aaggcacgta gaaaagtggc attaattcat gccaaccaaa
    24001 gaaaaatgaa aggaatacat gtatagttgg agccatcaac aatgatatgg aatataaaat
    24061 agcaactctc gccttgtcat gcgtagtgca attggtggca aagcgtatgt aataaatgca
    24121 gggatgccat agcatatcca ccacggcatt tctttcccac acgaaatttt taaaatgtca
    24181 tttcacctac aacggcaata acagatagta gtgtacaaac acaattaatg caacaagcat
    24241 tgccttgtat gattaaactt gtcaacctgc ctaccccgca tccggcttac tccgaatcct
    24301 tatcgtacca ggataactgc gcaaataagc tatactgact gttctgccca ctgtagt
//
LOCUS       DAOPHX010000021        23708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_2216_length_23708_cov_5.757874, whole genome shotgun sequence.
ACCESSION   DAOPHX010000021 DAOPHX010000000
VERSION     DAOPHX010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 23708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23708
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2216_length_23708_cov_5.757874"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..288)
                     /locus_tag="PK502_02875"
     CDS             complement(88..288)
                     /locus_tag="PK502_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPG99794.1"
                     /translation="MKGKVKWFDAAKGFGFIQTEEGSDVFVHYTGIAKDGFKRLDEGQ
                     EVSFEITEGKRGPQATNVVVLE"
     gene            532..1023
                     /locus_tag="PK502_02880"
     CDS             532..1023
                     /locus_tag="PK502_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPG99795.1"
                     /translation="MNISNDKVVSISYELKVNGDLVDSAVADNPLVFLYGHGQLLPLF
                     ETNIHNLKTGDSFEFSIPYADGYGEVDEEAIIDLPKDIFVIDGVLQEDMLVVGNTLPL
                     RDSEGNELNGTILEVGDTHVRMDFNHPLAGEDLYFTGKIIDVREATSEELSHGHVHAH
                     GHH"
     gene            1141..1674
                     /locus_tag="PK502_02885"
     CDS             1141..1674
                     /locus_tag="PK502_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99796.1"
                     /translation="MHRNRLVKLLIGIVLVAFTSCGGSDDDESDNFIRFGNEKFRVYL
                     GYQYKYSETLQETGSTPFVVSLLGEGVKYSTDNAAFTGTGSLIRLYMYSESNVEVKNG
                     DYPIDIFSTKQVYSADSCSVYFNYTFNVDTGKVYPVKAGVFKVENLGRLMSYKLNMVN
                     ADSLHFVGEYKGVFNTL"
     gene            1850..2404
                     /locus_tag="PK502_02890"
     CDS             1850..2404
                     /locus_tag="PK502_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HPG99797.1"
                     /translation="MLFGLILGIIAIPLGANQLVSDYIKPFGTIFLNLLKLVAMPLIF
                     ASLVTGIAGMTDVGKLSRIGIKTIAMYLITTLLAVTIGLSLVSLIKPGSYFSDDRRQE
                     FVSKYSLDISSRQQAAESISQDSPLQFLVDIVPENIVQAASSNTAMLQVIFFALLFGI
                     ALVLVETRRLKLLTILSRGLTRLS"
     gene            2407..3093
                     /locus_tag="PK502_02895"
     CDS             2407..3093
                     /locus_tag="PK502_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPG99798.1"
                     /translation="MVEIIMFFAPFGVFALMASLIVDFAGDSLSSSLQLFASLGMYAL
                     TIIIGLIFIIVIVYPLFLKIISGTGFGKFYKSMFPAQLVAFSTSSSAATLPVTMKQVE
                     SELGVHEDISGFVLPLGVTINMDGTSLYQAVATVFIAQVFGIDLTFSQLLTILLTATL
                     ASIGSAGVPGAGVVMLIIVLNSVGLPVEGLALILAIDRPLDMLRTVVNVTGDATIAVC
                     IAKSEGKLEA"
     gene            3175..4755
                     /locus_tag="PK502_02900"
     CDS             3175..4755
                     /locus_tag="PK502_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HPG99799.1"
                     /translation="MNFEEEVKRRRTFAIISHPDAGKTTLTEKLLLFGGAIHTAGAVK
                     SNKIKKTATSDFMEIERQRGISVSTSVMEFEYQGYKINILDTPGHQDFAEDTYRTLTA
                     VDSVIIVIDHAKGVETQTRKLMEVCRMRKTPVIVFINKLDRPGNDPFDLLDEVEKELG
                     IGVRPLSWPIGQGPTFKGVYNLFEQKLFLFSPDFKQKVADEVVEITDINSPELDKYIK
                     PYTAKLREELEIINEVYPPLDINTYLSGRVAPVFFGSALNNFGVKELLDCFLQLAPYP
                     RPAEADIRTISPFEQKFSGFVFKIFANMDPNHRNRIAFLKVCSGKFERNVPYYHVRMA
                     KTFRFSSPNAFLADKKSIVDVAYPGDIVGLPDSGNFKIGDTLTEGEIINFKGIPSFSP
                     ELFRYIENAEPLKYKQLAKGIDQLTDEGVAQLFTQNSNGRKIIGTVGALQFDVIQYRL
                     EHEYGAKCKYEPLNIFKACWIVSSNKAQLDDFRIRKYSNLATDKQGREVYLAESPYAL
                     QMAQDKFTDIKFYFTSEF"
     gene            5201..6550
                     /locus_tag="PK502_02905"
     CDS             5201..6550
                     /locus_tag="PK502_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG99800.1"
                     /translation="MAKKPKLRFWQIWNLSFGFLGVQAGFALQNANVSRILSNYGADL
                     HHLSFFWLLAPIMGLIIQPWVGAASDKTWNRMGRRKPFIFGGAIVATLAMLFMPNSQW
                     AVLIIPPVAFGAIMLALMDASFNVTFQPFRSLVADMTPAEQTNLGYSVQTLLINLGAV
                     IGSFLPYFLTEVLGIGNTAPEGHVPPSVIWSFYIGGALLLVSVLWTVFTTKEYPPKEF
                     AEYSGQTLEETPTKKGKSNFFKTLLSMPNIMHRLAVVQFFSWFALFTMWVYTTPAIAQ
                     HIWGTEIGDSTSFAYNEAANWVGVLFGLYSVFAALFAIFLNKIADKLGRIKTYSISLL
                     LGGLGFLSIYLFNNQYALIISMLGIGIAWAAILAMPYAILANSLPAERMGVYMGIFNI
                     TVVVPQIVSGIVLGTMLKSFFNDEAIYMILLAGFSMILASASAMLFVKDNSKVLPNE"
     gene            6543..7205
                     /gene="pgmB"
                     /locus_tag="PK502_02910"
     CDS             6543..7205
                     /gene="pgmB"
                     /locus_tag="PK502_02910"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HPG99801.1"
                     /translation="MSSIKGCIFDLDGVVVDTAKYHYQAWKRLANELGFEFDESHNER
                     LKGVSRMRSLEILLEVGGLSFPQEKMEQLAARKNEWYVEYISRMTPNEILPGSKELLE
                     ELRAKGIKLALGSASKNTPLIMDRIGLKDHFDAIIDGNIISIAKPAPDIFLAASKAIG
                     VEPPCCIVFEDAEAGVEAAINAGMRVVGIGNPQVLKRANLVVPNLKNFTFQYLVNSLS
                     EK"
     gene            7222..9546
                     /locus_tag="PK502_02915"
     CDS             7222..9546
                     /locus_tag="PK502_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HPG99802.1"
                     /translation="MNTYLKHDEWRIIEDNFIPENTRASESIFSIGNGAMGQRANFEE
                     KYSGNSLQGSYIGGVYYPDKTRVGWWKNGYPEYFAKVINSTNWIGISVKVNGEELDLA
                     TAKVLNFCRILNMKEGYLERSFTCELPNGIKLEVKSTRFLSMVDTEIGAIRYSIKPVN
                     SNASIQISSYLDADVHNEDSNYGEKFWDVIDVRGGEIFSYIISQTKKLDFQVGHAMRN
                     LLFINGQPQNLKPQVNSGQMYVDNRFTVNASSGDEVILYKYAAVISNLNHEKGKFTKV
                     LETLIEGASEKGFDKLFDEHRKAWADIWNHSDITIEGDVAAQQGIRFNIFQLSQTYTG
                     RDERLNIGPKGFTGEKYGGSTYWDTEAYCLPFYMVTHGQKVARQLLLYRYKQLGKAIE
                     NAHKLGYTDGAALYPMVTMNGEECHNEWEITFEEIHRNGAIAFAIYNYVRYTGDKDYL
                     VDFGLEVLIAIARFWSQRVNFSTEKGKYVMLGVTGPNEYENNVNNNWYTSTIACWCME
                     YAEEVSSYVKSTNNAKYQELCSRIGFDEVNEIARWKEIRSNIFLPEDKKLGIFLQQEG
                     YLDKEQILVKNLDPKERPINQKWSWDRILRSCFIKQADVLQGLYFLEDRFDTETIKRN
                     YEFYENRTVHESSLSPCVHSVLASRIGNVEKAYELYLRTARLDLDDYNNEVKEGLHIT
                     SMAGTWISIVEGFGGMRVRSNMLTFNPLIPQNWKSFSFKVWFRGNVLKIRVTNSELSL
                     FNEVGSNITVKIYGKDQLIPEGGAITIAVQKQVY"
     gene            9577..10638
                     /gene="fbaA"
                     /locus_tag="PK502_02920"
     CDS             9577..10638
                     /gene="fbaA"
                     /locus_tag="PK502_02920"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPG99803.1"
                     /translation="MHFKPGIITGNELNKLFEYAKQEGFALPAVNVTGTNTMNAVMEA
                     AHEVGSPVIIQFSNSGASFISGKSISNDGQRASILGAVSGAKHVHLLAEAYGVPVVLH
                     TDHAAKKLLLWIDGLLDMGEKYYKETGAPLFSSHMLDLSEEPLHENIEICKRYLERMS
                     RMGMTLELELGVTGGEEDGVDNTGVDNSRLYTQPDDVAYAYEELMKVSPNFTIAASFG
                     NVHGVYKPGNVVLNPIILKNSQELISRKFGKGSKPVNFVFHGGSGSEKEKIAEAISYG
                     VVKMNIDTDLQWAFWDGIKNYYQSKKEYLQGQIGNPEGEDSPNKKYYDPRVWLRKGEE
                     SVINRLKEAFADLNALNRA"
     gene            10775..12622
                     /locus_tag="PK502_02925"
     CDS             10775..12622
                     /locus_tag="PK502_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HPG99804.1"
                     /translation="MKKPLILMLMLIAACSMNVNSQNIKRVEPPCWWVGMKNPRLQLM
                     IYGSNINQAQVGISDSRVTLESVDKTENPNYLFLNLAINENAEPGAFNITFKFPKGKP
                     RVVAYELKERKQGSSERMGFNNSDVIYLVFPDRFSNGNPANDSIKGFSDKYNRNDPYG
                     RHGGDIQGIINHLDYFNQLGVTALWLNPILENNQPSTSYHGYAITDFYKVDPRLGDNE
                     LYRKFSQMAHERGLKVVKDMVFNHCGSSHWWMNDLPMHDWINQFPQFTRTNYRIPTTF
                     DPHASTVDSSLMADGWFDLTMPDLNQRNPYMATYLIQNSIWWIEYADLNGIRMDTHPY
                     CDKHFMSRWCAAVMSEYPNFSIVGETWVNYPDWVAYWQKDAHNQDGYNSNLKVAMDFP
                     LMFAMHKAFDEKDGWDTGLARLYEILAHDFVYPSPNNLLIFADNHDIGRFHRDSSLAL
                     GRLKLAMAFLLTTRGIPEIYYGTEIILPGDDAKGHGDIRRDFPGGWEGDLKSAFTTDG
                     RTNRQNEIFNYISTILNWRKTASAVHSGKLTHFIPENEVYVYFRFDDKQTVMVILNNG
                     YQPKMLETARFSEMMKGKNSAVDIISGKVFDNLEKIQLSPRSALILELR"
     gene            12658..15030
                     /locus_tag="PK502_02930"
     CDS             12658..15030
                     /locus_tag="PK502_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPG99805.1"
                     /translation="MKKLLFIISIATLAACQPSPNLDGVAEKPAVADLLSAIQLKGDS
                     TKVVLTDYFPDNKVVDSITIPDFFRYSYSPDSASITLVANNLNLPPLSIIQVWVKGNE
                     RYDLLVRKSKKLPVAFTFDPEGKKVKSVQLAGQINDWNPNATSLTFDGKVFRTTLLLN
                     PGKYHYQVVVNGKWMLDPANPLKEDNGMGGQNSVVEVGNLKPNMLPKLVALTAHKNIL
                     NIKATGTVDSIIVLWQNYLLTGNFIKRDGDVFHVTLPRNAEEYSRSYIRVWAYNEFGE
                     SNDLLIPLEGCRVVEDPGKLTRSDKHTQVMYFLMVDRFNNGTTANDRKVDDPEILPKA
                     NYFGGDLVGVTQKIKDGYFTSLGINTIWLSPITQNPLGAWGLYPNPRTKFSGYHGYWP
                     VSLTKIDFRYGTDGDLRELIDEAHSRNMNVILDYVANHIHVEHPLYKQHPDWTTSLYL
                     PDGSLNTERWDEYRLTTWFDVFLPTLDLQRPEVYEPMTDTALYWLTNFQLDGFRHDAT
                     KHIHENFWRRLTQKIKLKVTDRSIYQIGETYGSRELVASYIGSGMLDSQFDFNIYDAS
                     VAAFARSNYPFSSLNSSLLESFSYFGWNNLMGYISGNQDRGRFISYAGGALRFDEDAK
                     KAGWTREIGVGDTSAYSKLCMLNAFNLTIPGVPTIYYGDEFGMPGGNDPDNRRMMKFD
                     NLSPIENRTLDVVKKLVNLRRNTMALNYGNFETLLADSTQYAYARTYLQSSAIVVFNK
                     SDEDKSIIFKLPDYMVGRNFTSKFGSNFSIQNQIVKVDLKPNSFEILISK"
     gene            complement(15248..15320)
                     /locus_tag="PK502_02935"
     tRNA            complement(15248..15320)
                     /locus_tag="PK502_02935"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15285..15287),aa:Lys,seq:ttt)
     gene            15612..16418
                     /locus_tag="PK502_02940"
     CDS             15612..16418
                     /locus_tag="PK502_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPG99806.1"
                     /translation="MKGKIAIITGASSGIGKACAYEFASRGAKLVLAARNLEVLNSIV
                     QDLLLNGHEAIAVRTDVTVEDDCKNLVDEALKRYGKIDYLLCNAGISMRALFKDVDLK
                     VIRQLMEVNFFGAVYCTKYALPHLLASRGSVIGVSSIAGFHGLPGRSGYSASKFALQG
                     FLETLRIENLKNGLHVMVAAPGFTATNVRVSALTADGSPQGVSPRDEKHMMTPQKVAR
                     TIAHGILRKRRNIIMTTEGKLSVLAQRVFPKIIDRILYMFMAREPNSPFK"
     gene            16495..17967
                     /gene="cls"
                     /locus_tag="PK502_02945"
     CDS             16495..17967
                     /gene="cls"
                     /locus_tag="PK502_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPG99807.1"
                     /translation="MILLNFFMNVAMANLNRIILIIYFITVLFTIIMVIHEKRDPSKT
                     STWVLLIISLPVLGLILYVIFGQNRRKEKIFNRKEIQDIQQIDYLSRQQVTNFSKKGQ
                     LNSKISNHISIITLLLNNSKALLTDFNSVSIFFKGKPAFDSIIDELNNATSSIHLEFY
                     IINDDKIGNRIKEVLIAKARLGVKVRVIFDDVGSWSLPKKYIRELKQAGVEVFPFMKV
                     RFPLLTSKANYRNHRKIVLIDGRVAFLGGMNIADRYIEGSKIGMWFDTMLRIEGEAVH
                     SLQVIFLIDWYFVSGDLVADRPLYFPEVSVSTYHPIQVATSGPDSDWASIMQAFFLGI
                     TKASHHVYIATPYFIPNESILTALKTAALSGIDVQIMLPGISDSTIVYWSSLSYVSEL
                     LEAGIKVYLYRHGFNHSKLMMIDGSLAFTGSANMDIRSFEDNFEVAAIMYDDQITRLL
                     ESSFIQFRNSSQEITLEEWNRRSAGNSFKEAFARLISPLF"
     gene            18043..18714
                     /locus_tag="PK502_02950"
     CDS             18043..18714
                     /locus_tag="PK502_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPG99808.1"
                     /translation="MRKLFILVLALFSFTIANAQFLRFGVKGGVSSTNVKIDETSLQG
                     LTTTDGLKQFAVEQGNSKLGLHFGVFGRIQLAGLFIQPEILFTQSKGEVVLSYINSAD
                     EAIQTVEDQKFNKIDIPVMVGWKFGPARIGLGPVASIMLSENDGLKKELASLTSQSAE
                     SNFKNAVFGYQLVAGIDILKKITFDFKYEGNLSKLGEGITLGGSDFKFDQRNPQFIFS
                     LGIFF"
     gene            18925..20343
                     /locus_tag="PK502_02955"
     CDS             18925..20343
                     /locus_tag="PK502_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG99809.1"
                     /translation="MTILNVILGITCILLVGYVVLIHRRIHPRNITGSSDQLKDIEAL
                     KIENAELKIRCRELQVDNDELNSLLSTQALEQSRLREALERTKRKAEESDILKSNFLA
                     NMSHEIRTPMNGILGFAQLLKNEESAEKRDRYIDIIYHNGLMLVNLIDDIIDISKIDA
                     GQLAITKAECDLDNLLFEVYTFFNEVKFKQEKEHINIRLLNLNDGEANILYTDGLRLR
                     QVLVNLISNALKFTEKGFVEFGFVNRPEERQIEFFVRDTGIGIPTDKQDIIFERFRQI
                     QEGSTRKYGGTGIGLYISKSVVNLLGGDIWFESTCGVGTTFYFTIPYEDVNVAHSGAK
                     FYRTSSPEYSWEGRNILVAEDTESNYLYISTILAKTKANVIWARNGEEAVNAALRGAV
                     PVDLILMDIQMPVMDGYEAVTAIRRQNAEVPIIAQTAYALPHDNIRCFEVGCNDYISK
                     PINALLLKQKINELLNAYIKNS"
     gene            complement(20330..21136)
                     /gene="kdsA"
                     /locus_tag="PK502_02960"
     CDS             complement(20330..21136)
                     /gene="kdsA"
                     /locus_tag="PK502_02960"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPG99810.1"
                     /translation="MTTIKHLDSGNFFLMAGPCVVESEELAITVAEKVSAIAEKFKIP
                     YIFKASYRKANRSRIDSFSGIGDLKALKILQKIRTQLQLPVVTDIHSAEEAGIAAEYV
                     DVLQIPAFLCRQTDLLIAAAKTGRVVNIKKGQFVSPESMKYAAQKVVDSGNSKVILTD
                     RGTQFGYQDLIVDFRGIPQMRAFGFPVVVDVTHSLQQPNQTSGVTGGRPDLIETIARA
                     AVATGADGIFMETHPNPAVALSDGANMLDLKYMEELMNHLVQIRQVIRNF"
     gene            complement(21173..22453)
                     /locus_tag="PK502_02965"
     CDS             complement(21173..22453)
                     /locus_tag="PK502_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPG99811.1"
                     /translation="MSSIPLAERMRPHTLDDYVGQSHLVGEGSVLRDMVKSGNLNSFI
                     LWGPPGVGKTTLAKIVANELKRPFHILSAINSGVKDVREVIEKAKSQKFFNSPSPILF
                     IDEIHRFSKSQQDSLLGAVEQGVITLIGATTENPSFEVISPLLSRCHVYILKPLSNEE
                     LSLLLNRAISTDVELKKIKFKIAETEALYHFAGGDARKLYNIIELLASVAGGKGEVEI
                     SNEKVTKVLQENIALYDKNGEQHYDIISAFIKSVRGSDPNAAVYWLARMLEGGEDPKF
                     IARRLVILASEDIGIANPNALLLATSCFQAVHLIGMPESRIILSEASIYLATSAKSNS
                     AYEAIDKAQALVRTSGNLPVPLHIRNAPTKLMKEIGYGADYKYAHSFKGNFVDQEFLP
                     SGISGTTLYEPQDNPKEREIAMRLRNLWKDKYNY"
     gene            22654..23634
                     /locus_tag="PK502_02970"
     CDS             22654..23634
                     /locus_tag="PK502_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99812.1"
                     /translation="MKSHRRISMIIFLSTIVATFTVNAQENVVEVLSGNLNDAEKLAA
                     AYLEPFGKSLGSSLNNGWFNSAKPHGLFGFDITFTGAVTFPPSSAKNFDVAKLNLEYW
                     ELKNPSQNTSPTVSGEKVSGPILTDKATGLSELTLPKGSNMQFLPAPVIQVGLGLPFH
                     TEVVGRFFPKVDFPEVGNFSSWGIGVKNEFKEFIPGFKLIPIDVSVFFGYTEFTSTFD
                     IDKSKDQTLNFNASGFTGKLLVSKSIPVLTVYAGVGYVKSTTDVKLKGYYDIPNVGLN
                     MKDPLALDFSNNGFNANIGIKIKLALITFHCDYSFGDYSVLNTGIGISFR"
BASE COUNT         6642 a         4994 c         5119 g         6953 t
ORIGIN      
        1 gcaatcccat tttgcgattt accattacct tctaataaaa aaaggcctcc attggaggcc
       61 ttactggcta gtatgttagg aataaactta ttctaacact accacattgg tagcctgtgg
      121 gccacgtttt ccttcggtaa tttcaaaact tacctcctga ccttcgtcaa gacgcttgaa
      181 accatcctta gcaattcctg tgtagtgaac aaatacatca ctaccttcct cggtttgaat
      241 gaagccaaag ccttttgctg catcaaacca ttttacttta cctttcatgt taaaattgat
      301 ataaatgatg aattaaatta attaatagcg ttgcaaatat ggcatataaa attgaatatt
      361 caacattatt ctaaaaaaaa aaaattattt attgttaaaa tctttgcctc aagggatata
      421 catgctttta agtaaggtcg ctttttcatt cgaacatttg gggatttcta ttgtttttta
      481 ccgaaattag atattccttt ggcaattatt aattaaatca aatcaattta catgaacatt
      541 tctaatgaca aagttgtatc cattagctat gagttaaaag tcaatggcga tttagttgat
      601 tcggcggttg ctgacaaccc gttagttttc ctttacggcc atggccagct gctgcctctc
      661 ttcgagacca acattcacaa cctgaaaaca ggtgattcat tcgaattttc gattccctat
      721 gctgatggtt atggagaagt tgacgaggag gcaatcattg atcttcctaa ggatatattc
      781 gttattgatg gagtgctcca ggaagacatg ttggtagttg gaaacacatt gccgttgaga
      841 gattccgagg gtaacgagct aaatggcaca attctggagg tgggcgatac ccatgttaga
      901 atggatttca accacccctt ggctggagaa gacctttact ttaccggaaa aattattgat
      961 gtcagggaag ccacttcgga ggagctcagc catggccatg ttcacgctca tgggcaccat
     1021 taaactattc aattcgtaaa cactgacggg aaatttttaa tttcccgttt tttatttacc
     1081 ttttttgtaa taattttttt ttattttagc agggttaatt acaaccacat acctactcgt
     1141 atgcatagaa accggttagt aaaacttctt atcggaatag ttcttgttgc tttcacttca
     1201 tgcggaggta gcgacgatga tgagtccgat aatttcatcc gatttggaaa cgaaaagttt
     1261 cgtgtttact taggatacca gtacaagtat tccgaaacct tgcaggaaac cggaagcacc
     1321 cctttcgtag tatctctcct tggtgagggc gtaaaataca gcactgataa cgccgctttc
     1381 accggaacag gatccctaat taggctatat atgtactccg agagcaatgt tgaggtcaaa
     1441 aatggagact accccattga tattttttct accaaacagg tttattcggc cgactcgtgc
     1501 agtgtctatt tcaactacac cttcaatgtt gacaccggta aggtttatcc tgtaaaagca
     1561 ggggttttta aagttgagaa ccttggacgg cttatgagct acaagcttaa catggtaaat
     1621 gcggattccc ttcattttgt cggcgagtat aaaggtgtgt tcaatactct ttagcgtcaa
     1681 cctatgctca acttgtgcta tagaccggtt tgtccggtct ttttaatata tgtttaacaa
     1741 cagtagcctt tattagggta agtcaatgat tttaaagtag aaattttcaa cattcatatt
     1801 tatgagagga gcccgacgat taccactata tgccaaaatc ctgataggta tgttatttgg
     1861 ccttatactg ggcattattg caattcccct tggcgctaac cagctggttt ccgactacat
     1921 taagcccttt ggtaccattt tccttaatct tctgaagcta gtcgccatgc cgttaatttt
     1981 tgcttcgcta gttacaggaa ttgccgggat gactgatgta ggcaaactct ccagaatagg
     2041 gattaaaacc attgcaatgt acttaatcac taccttgctg gcggttacca ttggcctaag
     2101 cttggttagc ctgataaaac ccggtagcta cttcagcgat gaccgcaggc aggagtttgt
     2161 atctaagtat tcccttgaca ttagctctcg gcaacaagct gccgagagca tctctcagga
     2221 ttctccacta cagtttctgg ttgatattgt tcctgagaat atagttcagg ctgcatcgag
     2281 caatacggcc atgcttcagg ttattttttt cgctctgctt ttcggcatag ccttagtgct
     2341 ggtagaaacc cgaaggttga agttgttaac aattttatcc aggggcttaa cgaggttatc
     2401 ctgaagattg tagaaatcat catgtttttt gctccttttg gcgtttttgc tcttatggct
     2461 tcccttattg ttgactttgc tggggatagc ctctcttcct cccttcagct ttttgcctcg
     2521 ctgggaatgt atgccctcac tattattatt ggcctcatat tcattattgt tatagtttac
     2581 ccattatttt taaaaatcat ttccggtacg ggttttggaa aattctacaa atcaatgttt
     2641 cctgctcagc tggtggcttt ctcaaccagt tcgagcgcgg caacccttcc ggttaccatg
     2701 aagcaggttg agagcgagct gggcgttcac gaggatattt ccggctttgt tcttccactg
     2761 ggcgttacca tcaacatgga tggaacaagc ttgtaccaag ctgtagcaac agtttttatt
     2821 gcccaggtat ttggtataga tttaacattt tcgcagctgc taaccatttt gctaactgca
     2881 acccttgctt caattggttc agccggagtc ccaggtgcag gggttgtaat gctcattatc
     2941 gtgctcaatt ctgttggcct ccctgttgaa gggctagcac taatcctcgc cattgaccgc
     3001 cccctggata tgctgcgcac ggttgttaac gttaccggag acgccacgat tgccgtttgc
     3061 attgctaaaa gtgaaggtaa gctggaggct tgagaatatc accctagtgc ttatacattc
     3121 acttcaacac ccaatgctaa attttgtaca tttgcggctg caaaaaagta aggtatgaac
     3181 tttgaggaag aggttaagcg ccgtaggaca tttgccatta ttagccatcc agatgccgga
     3241 aaaactacac taacagaaaa gcttctccta tttggaggag ccattcatac tgctggcgct
     3301 gtaaaaagca acaagattaa gaaaactgcc acttccgatt tcatggagat tgaaagacaa
     3361 cgaggaatat ccgtgtcaac ctcggtaatg gagttcgagt accaggggta taaaattaac
     3421 atccttgata ctcccggcca ccaggatttt gccgaggata cctatcgcac tcttactgct
     3481 gttgacagcg ttatcattgt gatagatcac gccaaaggag ttgaaaccca aacccgtaag
     3541 ctgatggagg tttgccgtat gcgaaaaacc cccgtcatcg tttttattaa taaactcgat
     3601 cggccgggaa acgatccttt cgatttactt gatgaggtgg aaaaagagct aggtattgga
     3661 gttaggcccc ttagttggcc cattggacag ggcccaactt ttaagggtgt ttataacctt
     3721 tttgagcaga aacttttcct gttttcaccc gacttcaaac aaaaggtagc tgacgaggtg
     3781 gttgaaatta ctgatatcaa ttctcccgag ctggataaat atattaagcc atatacagca
     3841 aagctcaggg aggaactcga aattattaac gaagtttatc ctccgttaga tatcaatacc
     3901 tacctttcgg gcagggttgc tccggttttt ttcggaagcg ccctcaataa ctttggcgtt
     3961 aaagaacttc tcgattgttt tttgcagctg gcaccctatc ctagacctgc cgaggccgac
     4021 atccgaacaa tttcaccctt tgagcaaaaa ttcagcggct tcgtttttaa aatttttgcc
     4081 aacatggatc ccaaccaccg caataggatt gcctttctaa aagtatgctc gggtaaattt
     4141 gaacgtaatg ttccgtacta ccatgttcgc atggcgaaaa cattccggtt ttctagcccc
     4201 aacgcttttt tggctgataa aaaatcaata gttgatgtgg cttaccccgg tgatattgta
     4261 ggcttgcccg attcaggaaa tttcaagatc ggcgatacgc ttactgaggg tgaaattatt
     4321 aatttcaaag gtatccccag cttctctccc gagcttttca ggtatattga gaatgctgag
     4381 ccattgaagt ataagcagct ggctaagggt atcgaccagc ttactgacga aggggtggcc
     4441 cagctgttta cccaaaattc taatggtcgt aaaattatag gtacagttgg agccctccag
     4501 tttgacgtta tccagtaccg cctggagcat gagtacggcg caaagtgcaa gtatgaaccg
     4561 ctcaatattt tcaaagcatg ctggattgtc agcagcaata aagctcaact tgatgatttt
     4621 cgtattcgta agtatagtaa tcttgccacc gataagcagg gtagggaagt ttaccttgcc
     4681 gaatctccct acgccctgca aatggctcag gataagttca ccgatatcaa attctacttc
     4741 acttctgaat tttagaactg ccctttaact ccaaatgccc catataaata gggtgtgatt
     4801 ttgtgttgtt ttgctactga aattatgatc ctacctattt tcagtagaaa acagtaaaat
     4861 ttactagtgc aaacagccca aaatgcagga ataaccaccc aggcaattca caatactggt
     4921 ttggcactat ctatttgcct gaatatctga taggtagcta tgatttttct tttacctaat
     4981 gatgctactt ttttagatga actttagctt tattttcagc atggaaagct gttcgaagaa
     5041 aaattgaaaa tttttaaaaa aaaattattc caccgtaaac gattgaaatg ctgtttgcgt
     5101 gttctgaatc cctttgccta taaaggtttt aagttttcat taaggtttag ttaacctttt
     5161 ttaaattact tttggtgatt atgaaaaaat ttagtttgat atggcaaaaa aacctaaact
     5221 ccgtttttgg caaatttgga atctaagctt tggatttctt ggagttcaag ctggatttgc
     5281 tcttcaaaac gccaatgtat cgcgcattct ttcaaactat ggcgccgatc tgcatcatct
     5341 ttcgttcttc tggcttttag ctcctataat ggggttgata attcaacctt gggttggggc
     5401 agctagcgat aaaacatgga atcgaatggg gcgtcgtaaa ccatttatct ttggaggtgc
     5461 gattgttgcc acactagcca tgttattcat gcccaactcc caatgggcag ttctcattat
     5521 tcctccggta gcctttggag ctatcatgct tgccctaatg gatgcctcgt tcaatgttac
     5581 ctttcagcct ttccggtcgc tggtagccga tatgacgccg gctgagcaaa ccaaccttgg
     5641 atactccgtg caaaccctgc ttatcaattt aggagctgtt atcggttcgt ttttgcccta
     5701 tttccttact gaggttttgg gaattggcaa cacagccccc gaagggcatg ttcctccttc
     5761 agtaatatgg tcgttctata ttggcggtgc gctcctgctc gtttcggtgt tgtggacagt
     5821 attcacaacc aaagagtatc ctcctaaaga atttgccgag tatagcggtc aaaccctcga
     5881 ggaaactcct acaaaaaagg gaaaatcaaa ctttttcaaa accttgctct ccatgccaaa
     5941 catcatgcac aggttagcag tggtgcagtt cttttcgtgg tttgcccttt tcaccatgtg
     6001 ggtttataca actccagcca tcgcccagca tatctggggc actgaaatag gcgattccac
     6061 ttcctttgcc tacaacgaag cagccaactg ggttggggtt ctttttggac tgtacagcgt
     6121 atttgccgct ctatttgcca tttttcttaa taagattgcc gacaagcttg gaagaattaa
     6181 aacttattct atttctctgc tgcttggcgg cttgggtttt ctttccatct accttttcaa
     6241 taatcagtat gctcttatta tttccatgtt gggaataggt attgcatggg cagcaattct
     6301 tgcaatgccc tatgcaattc tggccaactc tctgcccgct gaaaggatgg gcgtttacat
     6361 gggaattttc aacattactg ttgttgtacc gcaaattgtt agtggtatcg tgctgggaac
     6421 tatgttgaag agttttttca acgatgaagc catctacatg attctacttg ccggattttc
     6481 catgatactc gcttctgctt cagcaatgct ctttgttaag gataatagta aagttttacc
     6541 aaatgagtag cattaaggga tgcatctttg atttggatgg cgttgtggtt gacacagcta
     6601 agtaccatta ccaggcatgg aagaggttgg ccaatgagct agggtttgaa tttgatgaaa
     6661 gccacaacga aaggttaaag ggggtcagca ggatgcgatc actcgaaatc cttcttgagg
     6721 ttggcggcct ttcctttccc caggaaaaga tggaacaact tgccgccaga aagaatgagt
     6781 ggtatgtgga gtacattagt cggatgaccc cgaatgaaat tcttcccggt tccaaagaac
     6841 ttctggaaga acttagagct aagggaatta agctggcgct tgggtctgcc agcaaaaata
     6901 cacccctaat catggaccga attggactaa aagatcactt tgatgctatt atagacggca
     6961 atatcatttc gattgctaaa cctgctcctg atattttttt ggcagcgtca aaagcaattg
     7021 gtgtagagcc tccatgttgt attgtatttg aagatgccga agccggagtt gaagcagcaa
     7081 taaatgcagg catgcgcgtg gtgggtatcg gaaaccctca ggtactaaaa agggcaaatt
     7141 tggttgtacc caacctgaaa aattttacat ttcaatattt agtcaattca ttatcagaaa
     7201 aatagtaaca tacatactct tatgaataca taccttaaac atgatgaatg gcgcatcatc
     7261 gaggataatt ttattcctga aaatacccgt gcttcagaaa gtatttttag catagggaat
     7321 ggtgcaatgg gccaaagggc taacttcgag gaaaagtaca gcggaaactc ccttcaggga
     7381 agctatattg gaggagttta ctaccccgac aaaacccgag tgggctggtg gaaaaatggc
     7441 taccctgaat actttgccaa ggtaatcaac tccactaatt ggattggcat ttcggtaaag
     7501 gtaaatggtg aggagctgga tttggctact gctaaagtgc ttaatttttg caggattctt
     7561 aacatgaagg aaggctacct cgaacgctca ttcacttgtg agctacccaa cggcatcaag
     7621 ctggaggtga agtcaacccg ttttttgagc atggtcgata ccgaaatagg tgctattcgt
     7681 tacagcatta aacccgtcaa ctccaatgct tccattcaaa ttagctccta cctcgatgca
     7741 gatgtgcata acgaagactc aaactacggc gagaaattct gggatgttat tgatgtaagg
     7801 ggcggtgaaa tatttagcta cataatcagc caaactaaaa aactcgattt tcaggtagga
     7861 catgccatgc gaaacttgct ttttattaat gggcaacctc aaaacctaaa accacaggtc
     7921 aactccgggc agatgtatgt tgacaacagg tttacggtta atgctagctc cggcgatgag
     7981 gtgatactct acaagtatgc ggctgttata tccaacctga accatgaaaa gggaaaattt
     8041 accaaagttc tagaaacact tattgagggg gcaagcgaaa agggtttcga caagctgttc
     8101 gatgagcacc gaaaagcttg ggcggatatt tggaaccata gtgatatcac cattgaaggt
     8161 gatgtggccg cccaacaggg tattcgcttc aatatttttc agctaagtca gacctacact
     8221 ggtagggacg aacgtctgaa tattggccct aaaggtttca ccggcgaaaa gtatggtggc
     8281 tctacatatt gggatacaga ggcatactgc ctgccatttt acatggttac ccacggacag
     8341 aaggttgccc gtcagcttct tctttaccgc tacaagcagc tgggaaaggc tattgagaat
     8401 gcccataagc tgggatacac cgacggagct gctctttacc caatggtaac catgaacggc
     8461 gaggagtgcc acaacgagtg ggaaattacc tttgaggaaa ttcacagaaa tggtgcaata
     8521 gcatttgcga tatacaacta cgttcgctac actggcgata aggattacct ggttgatttt
     8581 ggtctggagg tgctcattgc aattgcccgt ttttggtcgc aaagagtcaa cttttctaca
     8641 gaaaagggga aatacgtgat gctgggtgtt accggaccaa acgagtacga aaataacgtt
     8701 aacaataact ggtacacctc tacaattgcc tgctggtgta tggagtacgc tgaagaagta
     8761 tcaagttatg taaagtcaac caacaatgcc aagtaccaag agctttgcag caggattggt
     8821 tttgatgagg tgaatgagat tgcccgctgg aaggaaattc gctctaacat cttccttcct
     8881 gaagataaaa aattgggaat ctttctccag caggaaggtt acctggataa ggagcagata
     8941 ctggttaaga atttagatcc caaggaacgg ccaataaacc agaaatggag ctgggaccgt
     9001 attttacgtt cctgctttat taagcaggcc gacgttctgc aaggcctata ttttcttgag
     9061 gatagatttg acaccgaaac cataaagcga aactacgaat tttacgaaaa tagaacagtt
     9121 cacgagtctt ccctttcacc ctgcgtccat agtgtgctgg cttccagaat tggtaacgtt
     9181 gaaaaagcct atgagctcta cctgcgaaca gcccgcctcg atttggatga ttacaacaac
     9241 gaggtaaagg aaggcctgca tattaccagt atggccggta cgtggatttc aattgtggaa
     9301 ggatttggtg gcatgcgggt tcgcagtaat atgctcactt ttaatccttt aattccacaa
     9361 aactggaagt cattcagctt taaagtttgg tttcgtggta acgtcctcaa aataagggtt
     9421 acaaactcgg agttaagcct cttcaacgaa gtgggtagca acatcactgt taaaatttat
     9481 gggaaggacc agctaattcc tgaagggggc gctattacca ttgccgttca aaaacaagtt
     9541 tactaaaaca tctaataaag ctaaataatg gggaatatgc attttaaacc gggtattata
     9601 accggaaacg agttgaataa actattcgaa tacgccaagc aggaagggtt tgctctgccg
     9661 gctgttaatg ttaccggcac aaacactatg aatgctgtaa tggaagcagc tcatgaagtt
     9721 ggatcacctg taatcattca gttttcaaac agcggtgcat cgttcatctc aggaaaatca
     9781 atttccaacg atggacaacg cgccagcata cttggcgctg ttagcggcgc aaagcatgta
     9841 cacttgcttg cagaagccta cggggtacca gtagttttac acaccgatca tgcagctaaa
     9901 aagttactgc tctggattga tggacttctt gatatgggcg aaaagtacta taaggaaact
     9961 ggtgctcctc tatttagctc ccacatgctc gacctatccg aagaacccct tcatgaaaat
    10021 atagagattt gtaagcgcta tctcgaacga atgagtagaa tggggatgac ccttgaatta
    10081 gagctgggag taactggcgg cgaggaggat ggtgttgaca ataccggggt ggacaactca
    10141 cggctctaca cccaacctga tgatgttgcc tacgcctacg aagagttgat gaaagtaagt
    10201 cccaacttta ctattgctgc ttcgtttggc aacgtacatg gcgtttataa acccggaaac
    10261 gtagtgctaa atcctataat actaaagaat tctcaggagc taatcagcag gaaatttggt
    10321 aagggtagca aaccggtaaa ttttgtattc cacggtggaa gcggttccga aaaggaaaaa
    10381 attgctgaag ccattagcta cggtgtggta aaaatgaata tcgataccga tcttcaatgg
    10441 gctttctggg atggtattaa aaattactac cagtctaaaa aggagtacct ccaggggcaa
    10501 attggaaatc ccgaagggga ggattcgcct aacaaaaagt actacgaccc acgtgtatgg
    10561 ctccgaaaag gggaggagtc ggtgattaat cgcctaaagg aggcttttgc cgatttgaat
    10621 gcgcttaatc gtgcttaacc atgaacggat gcttcacagg ataccagtat tcattctgta
    10681 acttgagagt caacttttta acttgataag taatttattg gggttaatcc tctggggtta
    10741 accctttttg tttaacatta aaagcattat tactatgaag aaaccattaa ttttgatgtt
    10801 gatgctaatt gcagcatgtt caatgaatgt caactcgcag aatatcaaaa gggttgaacc
    10861 tccatgctgg tgggtgggca tgaagaaccc caggctccag ctgatgattt atggtagtaa
    10921 catcaaccaa gcccaggtag gaatctcgga cagccgggtt acactcgaaa gcgttgacaa
    10981 gactgaaaac cccaactacc tgtttctcaa tcttgctatt aatgagaatg ccgaacctgg
    11041 ggctttcaat attaccttta aatttccaaa gggaaaacca agggtggttg cctacgagtt
    11101 gaaagaacgt aagcagggtt cgtcggagag gatgggcttt aacaattccg atgtaatcta
    11161 cctcgttttt cctgaccgct tttcaaacgg caatcccgct aacgactcaa ttaagggttt
    11221 ctccgataag tacaaccgga acgatcctta tggcaggcat ggtggagaca tacagggaat
    11281 tattaaccac ttagactatt ttaatcagct aggcgttact gccctttggc taaatccgat
    11341 cttggagaat aaccagcctt caacttccta ccatggatat gccattaccg acttctacaa
    11401 ggtggatccc cgcttgggcg ataacgagct ttatcgtaaa ttctcccaaa tggctcacga
    11461 aagaggatta aaggtggtga aggatatggt ttttaaccat tgtggaagtt ctcattggtg
    11521 gatgaatgac ctgcccatgc acgattggat aaaccagttt cctcagttta cgcggacaaa
    11581 ttaccgtatc cccacaacat ttgaccctca tgcctcaact gttgatagta gccttatggc
    11641 cgatggctgg tttgatctta ccatgcccga cttaaaccag cgcaatccct atatggctac
    11701 ctaccttatc cagaatagca tatggtggat tgagtatgcc gatctcaacg gtattcggat
    11761 ggatacccac ccatactgcg acaagcattt catgtcgcgt tggtgtgctg ctgttatgtc
    11821 tgaataccca aatttcagta ttgttggcga aacatgggta aactaccccg attgggttgc
    11881 ctactggcaa aaggatgccc ataatcagga tggttacaac tcgaacctta aagtggctat
    11941 ggacttcccg cttatgtttg ccatgcacaa ggcttttgac gagaaggatg gttgggatac
    12001 cggacttgcc cgcctatatg aaattttagc tcatgatttt gtgtacccaa gccctaacaa
    12061 ccttctcatc tttgctgata atcatgacat tggtcgtttt catcgggact catccctagc
    12121 tcttggccgg ctaaaattgg caatggcatt tctacttact acccgtggaa tacctgagat
    12181 ttactatggc accgaaataa tcctgcctgg cgatgatgcc aagggacatg gcgatattcg
    12241 ccgcgatttt ccgggcggtt gggagggtga tttaaagtca gcatttacaa ccgatggcag
    12301 gacaaaccgt caaaatgaaa tttttaacta tatttctact atacttaact ggcgcaaaac
    12361 ggcttctgct gttcattccg gaaagttaac ccattttata cccgaaaatg aagtgtacgt
    12421 ttacttccgg tttgacgata agcaaactgt aatggtaatt ctcaataacg gctaccaacc
    12481 taaaatgctt gaaactgccc gcttcagcga aatgatgaag ggaaagaatt cagcggttga
    12541 tattatttcc ggtaaggtat tcgacaatct tgagaaaatt cagctctcgc cacgctccgc
    12601 tttgatcctg gagcttcgtt gattatacta aagttatcat ttcataaatg tcagtttatg
    12661 aagaagttat tatttattat ttcaatcgca accctagcag cttgtcaacc gtcgccaaat
    12721 cttgatggtg ttgcggaaaa accggctgtt gcggatttgc tttcagccat tcagctgaag
    12781 ggcgatagta ctaaagttgt tcttaccgat tacttccctg ataataaagt tgttgactct
    12841 ataactattc ccgatttctt tcggtatagc tactcacctg atagcgcttc catcactctg
    12901 gtagcaaaca acctaaatct gcctccatta agcattatac aggtgtgggt taaggggaat
    12961 gaacgctacg atttacttgt ccgcaagagc aaaaaacttc ctgttgcctt tacctttgac
    13021 ccggaaggta aaaaggtaaa atccgttcaa cttgccggtc aaataaacga ttggaaccct
    13081 aatgcaacca gcttaacatt tgatggtaag gtttttagaa caaccctgct gcttaaccct
    13141 gggaaatatc actaccaagt tgttgttaat ggaaagtgga tgctcgatcc cgccaacccg
    13201 cttaaggagg ataacgggat gggtgggcag aactcggttg ttgaagttgg caacttaaag
    13261 cctaacatgc tacccaaact tgtcgcatta acagcgcaca agaacatcct taacattaaa
    13321 gcaaccggaa cggttgattc tattatagtg ctgtggcaga attatttatt gaccggtaac
    13381 tttattaaaa gggacggcga tgtgtttcat gttaccttac cccgaaatgc cgaggagtat
    13441 agccgttcct acatccgtgt atgggcttat aacgagtttg gcgaatccaa cgacctgctt
    13501 atcccgctgg agggttgtag agttgttgaa gaccctggga agctaacccg tagcgataag
    13561 catactcagg ttatgtactt tcttatggtt gaccgcttca acaacggcac cacggctaac
    13621 gacaggaagg ttgatgatcc ggaaattctt cccaaggcaa actactttgg gggagacttg
    13681 gtaggagtta cacagaagat taaagatgga tattttactt ctttgggaat caatacaata
    13741 tggctatctc ctataaccca aaatcctctt ggagcatggg gtttgtaccc gaatccacga
    13801 acaaaattct ccggttatca tggatactgg ccggtttcgc ttacaaaaat tgactttcgc
    13861 tatggaacag acggcgactt gcgcgagttg attgatgagg cgcatagccg aaacatgaat
    13921 gtgattctcg actacgttgc caaccatatt catgtagagc acccccttta taagcagcat
    13981 cctgattgga caacatctct ctaccttcca gatggttcgc taaacaccga acgatgggac
    14041 gaatatcgcc taactacatg gtttgatgta tttctaccaa ctctcgacct acagcgtccc
    14101 gaagtttacg agccaatgac ggatactgct ctatactggc ttacaaattt tcaacttgat
    14161 ggtttccgcc acgacgctac caagcacatc catgagaatt tctggcgtag gcttacccaa
    14221 aagattaagc tcaaggttac cgatagaagc atctatcaaa ttggtgaaac ctatggtagc
    14281 agggagctgg ttgcaagcta tataggttcc ggaatgcttg attcgcagtt cgattttaac
    14341 atttacgatg cctcggttgc cgcgtttgct cgaagcaact acccattttc atcactaaac
    14401 agctctttgc tagagtcctt tagctatttt ggctggaaca acctaatggg gtacatttcg
    14461 ggtaaccagg atagggggcg ctttatctcc tatgcagggg gtgctttacg tttcgacgag
    14521 gatgctaaaa aggccggatg gacacgagag attggcgttg gcgacacatc agcatatagc
    14581 aaactatgca tgctaaacgc atttaacctt accattcccg gtgtccctac tatatactac
    14641 ggtgatgaat ttggcatgcc tggtggaaat gaccccgata atcgcaggat gatgaaattt
    14701 gataaccttt caccaataga gaacaggaca cttgatgttg taaaaaaact ggtgaacctt
    14761 agaagaaaca ccatggccct taactatggt aactttgaaa cccttttagc cgactcaacc
    14821 cagtatgctt atgccagaac atacctgcaa agttctgcaa tagttgtctt taataagagt
    14881 gatgaggata aatctataat ttttaaattg cctgattata tggtgggtag aaactttacc
    14941 tcaaagtttg gctcaaattt ttccattcaa aaccaaattg ttaaagtaga tttaaagcca
    15001 aactcatttg agatactcat ttccaagtag atacgttttt tattactcct aataagagcc
    15061 ctacatcgta gggctttttt gttgagcatt agcttattaa ggcaatggaa acaggaatcc
    15121 ggctgtagga agtaaaaatt tgaaatctac gctctattta atttgcaggc tgtgaatttt
    15181 ggaatctgta cataaaaaaa ccatccacta tgattatgga tggtaatgtt gaatagccta
    15241 taacttgtga tccagctggg attcgaaccc aggaccccat ccttaaaagg gatgtgctct
    15301 accagctgag ctactgaatc cttctatcaa atcttattat aacttatgtt acataagttt
    15361 gcctaaaggc ctgcaaattt aattaatttt ttacctaaac atccaatttc ttacgttgaa
    15421 ttatttacaa accaaataaa aaatttatac aacattgaaa atgagcacat cgcattataa
    15481 tcttgtgcaa gatatcttta aaagtttcgc aacactttct aattctccat gttttactta
    15541 tttttagtgc ggagaatatt tttttaaact ttagtatatt atagctaatt tttatgtcaa
    15601 ctcaaactag catgaaaggg aagattgcca ttataacagg cgcttcttca ggtattggaa
    15661 aggcttgcgc atacgaattt gcatcaagag gtgcaaagct ggttttagct gcccgtaatc
    15721 ttgaagtgct gaatagcatt gttcaggatt tgttgcttaa cgggcatgaa gccattgcag
    15781 ttagaaccga cgtaacagtc gaggatgatt gtaagaattt agtagatgag gctctgaaac
    15841 gatacggaaa aatcgattac ctgctttgca atgccggcat ttcgatgcgt gcacttttca
    15901 aggatgttga tttaaaggta attagacagc taatggaggt taactttttt ggcgctgttt
    15961 actgcaccaa gtatgcctta ccccaccttc tggcaagtcg cggctctgtt attggagtta
    16021 gctcaattgc tggttttcat gggctgccgg gtcgctcagg atattcagct tctaaatttg
    16081 cccttcaggg ttttctggaa acactccgta tcgagaatct taaaaatggc ctccatgtga
    16141 tggtagcagc cccaggattt actgcaacca acgtcagagt ttccgctctc acggcagatg
    16201 gaagcccgca gggcgtgtct ccccgcgacg aaaagcacat gatgactccc cagaaggtgg
    16261 ccagaacaat tgctcatggg atactgagga aaaggcgaaa tattatcatg acaaccgaag
    16321 gtaagctatc tgtgctggcg caacgggtct tccctaagat tattgaccga atactataca
    16381 tgttcatggc ccgagaacca aattctccgt ttaaatagcc gttttacctg cagctattta
    16441 ttaagctgtc aaattcctat tttttttata cattggttgg ctaaatcata ggcaatgatt
    16501 ttgcttaact ttttcatgaa tgtcgctatg gccaacctta accggattat tctgattatt
    16561 tattttatta ccgttctttt caccatcata atggttattc atgaaaagcg cgacccttct
    16621 aagacttcta catgggttct tctgatcatc tcccttcctg ttctaggact tattctctac
    16681 gtaattttcg ggcaaaatcg ccgaaaggaa aaaatattta accgaaagga aattcaggat
    16741 atacaacaaa ttgactacct gagccgacag caggttacca atttcagcaa aaaaggccag
    16801 ctaaatagca aaatctcaaa tcatataagc attataacgc tacttttgaa taatagtaaa
    16861 gccttgctta ctgatttcaa cagcgtttcc atattcttca aaggaaagcc agcttttgat
    16921 tccattattg atgagcttaa caacgcaact agtagcattc acctggaatt ctacattatt
    16981 aacgatgata aaattggtaa ccggattaag gaggttttga tagccaaggc caggctgggc
    17041 gttaaggtaa gggttatttt tgacgatgtg ggcagctgga gcctaccaaa aaagtatatc
    17101 agggagctaa aacaggcagg tgtagaggtt ttccctttta tgaaggtaag atttccgcta
    17161 ctcaccagta aggcaaacta ccgtaatcac cgtaaaatag tgcttattga tggtcgtgtg
    17221 gcttttctgg ggggcatgaa cattgccgac cgctacattg agggtagcaa aatagggatg
    17281 tggtttgaca ccatgcttcg aattgagggt gaagcggtgc attccctcca ggtgattttt
    17341 cttattgatt ggtattttgt ttctggcgat ttggttgccg atcgtccact ttacttccct
    17401 gaggtatcgg ttagcacgta ccaccctata caggtagcaa ctagcggtcc cgattctgac
    17461 tgggcaagca tcatgcaagc gtttttcctt ggtataacaa aagcgtctca ccatgtatat
    17521 attgccactc catattttat tcccaatgaa agtattctta cagcccttaa aacggctgca
    17581 ttgagcggta ttgatgttca aattatgctt cctggtattt ccgactctac tattgtttac
    17641 tggagttccc tttcctatgt ttctgaactc cttgaggcag gaattaaggt ttatctttac
    17701 cggcatggct ttaaccattc aaagttaatg atgattgatg gctcgttggc ttttaccggt
    17761 tcagcaaata tggatattcg aagcttcgag gataactttg aagttgctgc aattatgtat
    17821 gacgatcaaa ttacccgctt gctggaaagt tcattcattc agttcagaaa tagcagccag
    17881 gagatcaccc ttgaagaatg gaacaggaga tcggctggta acagctttaa ggaggctttc
    17941 gcccgtttaa tcagccccct tttttaatgt ttagcatgat atttgtatga gtggttatct
    18001 taataataag caactttaaa gtctaacctt aaattttatc ctatgagaaa gctatttatt
    18061 ttagtcctag cccttttttc attcacaatt gcaaatgctc agtttctgag atttggtgtg
    18121 aaaggaggcg taagttctac caatgtaaaa attgacgaaa ccagcctaca ggggctcact
    18181 acgactgatg ggcttaaaca atttgctgtt gaacagggca actctaagct aggtcttcat
    18241 tttggcgttt ttggaagaat acaacttgcc ggacttttta tccaacccga aattctattt
    18301 acccagtcaa aaggggaggt tgtgctgtca tacataaata gtgccgacga agctattcaa
    18361 accgtagaag atcaaaagtt taacaagatt gatatccctg ttatggtagg ttggaagttt
    18421 ggcccagcaa gaatcggact aggacctgtt gcatccatta tgcttagcga aaacgacggc
    18481 ctcaaaaagg aacttgcctc gctaaccagc cagtcagccg aaagtaattt taaaaatgcc
    18541 gtgtttggat accagctggt agccggcatt gatattctta agaagataac ctttgacttt
    18601 aaatacgaag gaaacctgag caagctgggc gagggtatta ccctgggagg tagtgatttt
    18661 aaattcgacc aacggaaccc ccagtttatt tttagcctgg gaatattctt ttaagacttc
    18721 taaaagggaa cgtttaaacc tccattttcc gatggaggtt tttttatcaa ctcttaggat
    18781 acgttaataa acaatatccc catgttaaag taaaaatggg tatttttaaa ggtgaacatt
    18841 tttatttacc ctaaatttgt gctgctaggt tttgattact ggctggttac ttacaacaga
    18901 ttgtatttta gattattaaa taacatgact atactgaacg tgatattagg aattacctgc
    18961 atactactag taggatatgt agttttaatt caccgtagaa ttcaccccag gaacataaca
    19021 ggtagttcag accagctaaa ggatattgaa gcattaaaaa tagaaaatgc cgagttgaaa
    19081 attagatgca gagagcttca ggtagataac gatgagctaa acagccttct ttctacacaa
    19141 gccttagaac aatccaggtt aagagaggca cttgaaagaa ccaagcgcaa ggctgaggag
    19201 tccgatatac ttaaatctaa cttcttagcc aacatgagcc acgaaatccg aacacccatg
    19261 aatgggattc tggggtttgc ccagctgttg aagaatgagg aatcggctga gaaacgtgat
    19321 cgctatattg acatcatcta ccacaacggg ttgatgctgg taaacctgat tgatgacatt
    19381 attgacattt caaagattga tgcgggccag ctggccatca ccaaggcgga atgcgatttg
    19441 gataacctcc tgtttgaggt gtacacattc tttaacgagg taaagtttaa gcaggaaaag
    19501 gagcacatca acatccggct gctgaacctg aatgatggtg aggccaacat tctttacacc
    19561 gatgggctcc ggctccgaca ggtgctggtc aacctcatca gcaacgccct caagttcacg
    19621 gagaagggtt ttgtggagtt tggcttcgtg aaccgtcccg aggagcggca aatagagttt
    19681 tttgttcggg atacgggtat tggcatccct accgacaagc aggatataat cttcgagcgc
    19741 ttccggcaaa ttcaggaggg atcgacccgc aagtatgggg gaaccggcat tgggctatac
    19801 atcagcaaaa gcgtggtgaa cctgctcggt ggggatatct ggtttgagtc aacctgcggg
    19861 gtgggaacta ccttctactt caccatcccc tacgaggacg taaatgtagc ccattcgggg
    19921 gccaagttct acaggacgag ctcgccggag tacagctggg agggtaggaa tatccttgtg
    19981 gccgaggata cggagtccaa ctacctgtac atatcaacca tacttgccaa aacaaaggca
    20041 aacgttattt gggccaggaa tggcgaggag gctgttaatg ccgctttgag gggagcagtt
    20101 ccggttgacc tcatcctgat ggatatccag atgccggtta tggacggcta tgaggcggtt
    20161 accgccatca gaaggcagaa tgccgaagtc cccataatcg cccaaactgc ctatgcctta
    20221 ccccacgata acatccgctg ctttgaggtt ggatgtaacg actacatttc aaagcctatt
    20281 aatgccttgc ttctcaagca aaagattaat gagctactaa acgcttacat taaaaattcc
    20341 tgattacctg cctgatttga accaaatggt tcattaattc ttccatatat tttaaatcca
    20401 acatgttagc accatctgaa agtgcaacag caggatttgg atgggtctcc ataaaaatac
    20461 catcggcacc ggtagctact gctgcccgtg caatagtctc tatcaggtcg ggtcttcccc
    20521 ctgtaactcc acttgtttgg ttaggctgct gtagcgaatg ggttacatcc acaacaacag
    20581 gaaatccaaa ggctctcatt tggggtatcc ccctaaagtc aactatcaaa tcctggtagc
    20641 caaattgggt gccccggtca gtaaggatta ccttactatt tccggaatcc acaacttttt
    20701 gcgctgcata cttcatggat tcaggtgaaa cgaattgccc ttttttgata tttaccaccc
    20761 tacctgtttt cgccgcggct attagcaggt cggtttggcg gcagaggaaa gccggtatct
    20821 gcaaaacatc cacatactcg gcagcgatgc cagcttcttc ggcgctgtgg atatcggtaa
    20881 ctacgggtag ctgtagctga gttctaatct tttgaagtat cttcagtgct ttcaagtcgc
    20941 caatacccga aaatgaatca attctagagc ggttggcttt tcgataggag gctttaaaga
    21001 tgtagggtat cttaaatttc tccgctatgg cgcttacttt ttctgcaacc gttattgcca
    21061 gctcctcgct ttctacaaca catggtccgg ccattagaaa aaaattcccc gaatcaagat
    21121 gcttgattgt ggtgattgag tttaaaacat tcatatacag gtgttttaca aatcaataat
    21181 tatacttatc tttccagagg ttcctcaacc tcatggcaat ttccctttcc tttgggttgt
    21241 cctgtggctc atataatgtt gtccccgata tgcctgaggg caaaaactcc tgatcaacaa
    21301 aatttccttt aaaactgtgg gcgtacttgt agtcggcgcc atagcctatt tccttcatta
    21361 gctttgttgg ggcattccga atgtgcaggg gaacgggtag gttgccggaa gttctaacta
    21421 gcgcctgagc cttgtcaatt gcttcataag cagaattact ctttgcagag gtagcaagat
    21481 atatcgaggc ttctgatagg ataattctag actctggcat accaataaga tgtactgcct
    21541 gaaagcagct ggttgccagc agaagtgcat ttgggttggc gattccgata tcctctgatg
    21601 ccagtataac aagtctgcgg gcaataaact ttggatcctc acctccctca agcatacgag
    21661 ctagccagta aacagctgcg ttgggatcgc tacccctaac agattttata aacgcagata
    21721 tgatatcgta atgctgctcg ccattcttat cgtagagcgc aatattttcc tgtagaactt
    21781 tggttacctt ctcattggat atctccactt cacctttacc accagcaaca ctggctagta
    21841 gttcaataat gttgtaaagt tttcgagcat ctcctccagc aaaatggtat aacgcctcgg
    21901 tttcagcaat tttaaatttg atttttttta actcaacatc cgtgctgatg gccctgttga
    21961 gtaaaaggga tagctcttcg ttgctgagtg gtttaagtat gtaaacatgg cagcgtgaaa
    22021 gtagtggtga aattacctcg aaggacgggt tttcggttgt ggccccaatt agggtgatta
    22081 caccctgctc aacagcacct aaaagtgaat cctgctgaga tttgctaaat cggtgaattt
    22141 catctatgaa cagaattggt gagggtgaat taaagaattt ttgagacttg gccttttcaa
    22201 ttacctccct aacatcctta acccctgagt ttattgcact aagaatatgg aagggccttt
    22261 ttagctcgtt ggcaacaatt tttgccaggg ttgtttttcc cacccccgga gggccccaaa
    22321 gaataaagga gttcaggtta cccgatttta ccatatccct tagcacgcta ccttccccaa
    22381 ccagatgtga ttgaccaaca tagtcgtcta acgtgtgagg gcgcattcgt tcggctagcg
    22441 ggatactgct catgctacct tatcttttct acaaaaatag caactaatag cttaccaacg
    22501 atatttttta agttaatgat gcttacttta cgaaacattc gtacttttgc ttatgattta
    22561 ggaaaataat ctaacttaat tttgaaaact gatttgatta gagctttgct gtaaaatatg
    22621 cctcccatta accttttaaa taaatgtgaa aagatgaaaa gccatagaag aatttcgatg
    22681 attatattcc tgagcacaat tgttgccaca ttcactgtta atgctcagga aaatgtagtt
    22741 gaggttttaa gcggaaacct taacgatgct gaaaaactcg cagcagccta cctcgagcct
    22801 tttggtaaga gtctgggctc aagcctgaac aacgggtggt ttaattctgc caagcctcat
    22861 ggactttttg gtttcgatat cacctttacc ggggcagtaa catttccgcc atcatccgct
    22921 aaaaattttg atgtggccaa gcttaacctt gaatactggg agttgaagaa tccatcccag
    22981 aatacttcac ctactgtttc cggtgaaaag gtaagcggcc ctatacttac cgataaggcc
    23041 acaggactat ctgagctcac cctgccaaag ggttcaaaca tgcaatttct tcctgccccg
    23101 gtaattcagg ttgggctggg tttgccattt cacaccgagg tggttgggcg ctttttcccc
    23161 aaggtcgatt ttcctgaggt aggtaacttt agctcctggg gaattggtgt aaaaaatgaa
    23221 tttaaggaat ttattccggg atttaagctg atacctatag atgtttcggt ttttttcggc
    23281 tacaccgagt ttacctcgac ttttgatatt gataaaagca aagatcaaac ccttaacttt
    23341 aacgcttcgg ggtttaccgg caagctgctg gtttcaaagt caattcctgt tcttaccgtt
    23401 tatgctggcg tcggatacgt aaagtcaact acagatgtca agttgaaggg atactatgat
    23461 attcccaatg ttggattaaa catgaaagat ccacttgcgt tagacttctc taataatgga
    23521 tttaacgcca atattggtat aaagataaaa cttgcgttga ttacgtttca ctgcgattac
    23581 tcctttggcg attattctgt tcttaacacc ggcataggaa ttagcttcag ataacttgct
    23641 aatggatagt actaaaaaag agagtcgctg ataagcgact ctcttttttt tatttcgttt
    23701 ttcgacct
//
LOCUS       DAOPHX010000022        23267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_2288_length_23267_cov_5.787782, whole genome shotgun sequence.
ACCESSION   DAOPHX010000022 DAOPHX010000000
VERSION     DAOPHX010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 23267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23267
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2288_length_23267_cov_5.787782"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..1724
                     /locus_tag="PK502_02975"
                     /pseudo
     CDS             48..1724
                     /locus_tag="PK502_02975"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
     gene            1806..2183
                     /locus_tag="PK502_02980"
     CDS             1806..2183
                     /locus_tag="PK502_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPG99813.1"
                     /translation="MKKIINTNDAPRAIGPYSQAVEANGFLFVSGQVPVNPSTGKIVE
                     GGIAEQTEQVVKNIGAILAKAGLTYANVVKTTCLLSDMENFATMNEVYARYFTAEMPA
                     RAAFGVVKLPLGALVEIECIAIR"
     gene            complement(2231..2677)
                     /gene="rplI"
                     /locus_tag="PK502_02985"
     CDS             complement(2231..2677)
                     /gene="rplI"
                     /locus_tag="PK502_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPG99814.1"
                     /translation="MEIILLQDMPSLGSKNDIINVRNGYARNYLIPKGFATIATESAK
                     KVLAENTKQRAHKEAKIKADAEELAKKLNGLKLTIGAKTSSTGKIFGSVNTIQISEAL
                     AEKGFEIDRKLIFIQDDQIKEVGSYTAEVKLHKEVKVSIEFDVVSE"
     gene            complement(2699..2968)
                     /gene="rpsR"
                     /locus_tag="PK502_02990"
     CDS             complement(2699..2968)
                     /gene="rpsR"
                     /locus_tag="PK502_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPG99815.1"
                     /translation="MSQNQSEIRYLTPPTVEVKKKKYCRFKKNRIKYIDYKDPEFLKK
                     FLNEQGKILPRRLTGTSQKYQRKVSTAIKRARHLALLPFVTDLMK"
     gene            complement(2972..3331)
                     /gene="rpsF"
                     /locus_tag="PK502_02995"
     CDS             complement(2972..3331)
                     /gene="rpsF"
                     /locus_tag="PK502_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPG99816.1"
                     /translation="MNHYETVFIATPVLSEAQMKETVTKFKDFITQDGGEIIHEESWG
                     LRKLAYPIQKKTTGFYHLLEFKAPGELIDKLETQYRRDERIIRFLTVKLDKYAVEYAE
                     KKRSNKVEKVEPKTMED"
     gene            complement(3484..4179)
                     /locus_tag="PK502_03000"
     CDS             complement(3484..4179)
                     /locus_tag="PK502_03000"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPG99817.1"
                     /translation="MDNLVEQLEVKFLQKGNPAVALQQKKYMRNQFEFFGIQSADRRE
                     IQKLLFTPEHLPAKNELGSTINRLWLKPQREFQYFAQEMAYKFHKQLELADISLYENM
                     VTTKSWWDTVDYIASRLMGEYFASFPEQRKPFIQKWVTSDNLWLKRSALIFQLNYKQQ
                     TDEVLLAATIHSLLPSSEFFICKAIGWVLRNYSKVNPEWVLQFVAHTPLPPLSKKEAT
                     RLIVQPPLRHKQV"
     gene            complement(4316..8566)
                     /gene="glgP"
                     /locus_tag="PK502_03005"
     CDS             complement(4316..8566)
                     /gene="glgP"
                     /locus_tag="PK502_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPG99818.1"
                     /translation="MNDKLLKPDYLFEVSWEVCNKVGGIHTVMSTKASMIENELKNNY
                     ITVGPDLIKGIEHNAEFIEDTNLFAAWRSHAIENGMPIRVGRWNIPCKPVAILVNFSQ
                     LISKKDEIFKGFWEKFKLDSITGQWDYIEPALFGYAAGKVIESFTNFNLSLRHRIVAQ
                     FHEWMTGTGLLYLKANVPQVGTVFTTHATVLGRSIAGNNIPLYSNIEKIDPESKAREF
                     GVISKQSLERISAQQADCFTTVSDITARECSHFLKKDVDLLTPNGFDDTFIPEAEEHK
                     LRRVEGKIKFYEVAEAMLAHEVAKDSMIVGISGRYEFKNKGIDVFLDALGKLNRDNNL
                     GREVLAFLLIPAGHHGPRKDVFHNLLEKEKDSNYSILDDTHVTHYLNDPDSEPILKKI
                     RALGLNNSSSDKVKVFWVPCYLNGNDGIFNKSYYDLLIGMDFTVFPSYYEPWGYTPME
                     SLAFKVPTITTSLAGFADWVINNYKKPKDAITIIKRNDENDLEVTVQIASTIDAFTRI
                     THERYNELMNNAQEIASIALWKNFIDYYYKAYDKALSLVGSRTHQFMELERQEILPTV
                     ERKFRTTQPSWTRLIVQKNLPEKLKPLDELSRNLWWSWNTDSKKLFESIDKELWIKCE
                     KNPILFLERISLPRFQELEHDAEFVEKLHSIYLNFTAYMMQRYQRKGPKIAYFSMEFG
                     LHSSLKLYSGGLGILAGDYLKEASDRNIDMVGVGLLYRYGYFTQQLSTSGQQIAVYDQ
                     QNFTQSLALPVRDEDGRWVTVSVAFPGRQVQARIWKVDVGRTELYLLDTDFEENLVND
                     RTITHYLYGGDLENRFMQEMILGIGGIRALNELGIKADIYHLNEGHAAFTGLERLRKF
                     ILDEKLSFSEAMEVVRATSLFTTHTPVPAGHDAFPEELVRTYMSHYPDRLMISWDQFM
                     DLGRMVPGDSTQRFSMSVLATNLAQEVNGVSWLHGEVSRKMFAGMWPSYFPNELHISY
                     VTNGVHFPTWTAREWKSIMENGQATNSNGYKTPAWNELENIPDSKIWEIRNTLRKRLI
                     KLINNRLSNPEMTRFESPRQVIEIQEKLSDSILTIGFARRFATYKRANLLFKDLDRLD
                     QLVNNPEHPVQFLFAGKAHPNDKAGQDLIKSIIEIAKQPRFLGRILFLQNYDMELSRH
                     MIQGTDVWLNTPTRPMEASGTSGQKAVMNGVLHFSVLDGWWVEGYRENAGWALPMEST
                     YAESEFQDELDAEMIYTILENEIVPAFYQRNSDDIPDKWISFIRKSMTQVASNFTTLR
                     MINDYQNRFYNKLYFRSSELIENDFEVAKKISSWKRRITRNWDEIEIIQVKQFDVSRE
                     AIIMGKEYTAEVILDMGSLGPDEIGVEMVLADLIEGGEVKVKRVHEFQVQAVEGSRVT
                     YRLNLIPVEPGAFECGIRIYPKNPMLPHRMDFPLVRWA"
     gene            complement(9106..10281)
                     /locus_tag="PK502_03010"
                     /pseudo
     CDS             complement(9106..10281)
                     /locus_tag="PK502_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
     gene            complement(10378..11670)
                     /locus_tag="PK502_03015"
     CDS             complement(10378..11670)
                     /locus_tag="PK502_03015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPG99819.1"
                     /translation="MRVLMFGWEFPPHITGGLGTACFGLTRGLTKQKVDVIFVVPKAY
                     GDEDKSAVRLVAAEDVDVAYSAKNLEEFWRHLKYLEISSNIVPYVDPEEYAKIIEHNE
                     AETVEYESGKVKTRFKFSGKYNPNLMQEVTRYAVVAANLAAREKFDVIHSHDWLTFPA
                     GIAAKMVSGKPLVVHVHATEFDRSGENINQAVFDVEQRGMMVADKVITVSNYTRNIVI
                     TRYGIPEDKVVTVHNAVDFSDEQDMEFERGVSEQIVTFLGRVTFQKGPDYFVEAANKV
                     LKRTHNVRFVMAGSGDMLNRMIRRVAQLGIAHKFHFTGFLKGPDVNRMFHLSDVYVMP
                     SVSEPFGISPLEAMRSSVPVIISKQSGVSEVLKYAIKVDFWDIDALADSIYGLLNYNA
                     LSTFFIRHGQEEVNSLKWDNAASKVIEAYQDVLSRKNS"
     gene            complement(11681..13624)
                     /locus_tag="PK502_03020"
     CDS             complement(11681..13624)
                     /locus_tag="PK502_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HPG99820.1"
                     /translation="MKYLAFDKDQLVNLEYSLEREILSTNRAGGYLSTTIVLCNTRKY
                     HGLMILPIDEFDGENHVLLSNVDETVVQHEKVFNLGIHRYPGIYEPRGHKYIVDFEYE
                     PTPTLVYRVGGVILKKELLFVHNEEQVMIRYTLLDAHSPTKLRIKPFMAFRNVHSLSK
                     ANMWANTKYQECSNGVRYKLYTGFPFLYMQLNRQNEFVASPDWYYNVEYMEEIRRGYE
                     GHEDLFVPGYFETSIRKGESIIFSAATNEQSTPALLRKFNSELTRRPPKDSFMNCIAN
                     TARQFIIQRGKRYDIIAGYPWFSSWSRDSFISLPGLTLGLDDVKTCKALLDSLSTDLK
                     NGLFPNVRTGNSEAFNSADAPLWYFWAIQQYASYTQNYTEVWDDYGKKMKAILKGYRE
                     GLPFSIKMQPNGLIWAGEKGYAITWMDAVIQAKPVTPRIGYPVEINALWYNALMFTLE
                     LATKNKDLRFVKEWQPIADQVKKSFNELFWIPEKNYLADYVDENGQNWDIRPNQLFAV
                     SLPYSPIDDEKKIAVLNVVSHELYTPKGLRTLSPNNPNYKGRYEGDQPTRDMAYHQGT
                     AWPWLLGAYVEAQFKLQGKGALGLAQEIVDSLQEDIALHGICSVAEVYDGDPPQRPNG
                     CISQAWSVAELIRIVKMIEKYSD"
     gene            13928..15091
                     /locus_tag="PK502_03025"
     CDS             13928..15091
                     /locus_tag="PK502_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPG99821.1"
                     /translation="MSDIGEQWVYVGGWNGLGSDITDSFKVSTRGRGNFSLQTTTPEM
                     LALSFDRVHFPILLCVEPGSNVEVTGTASSYQVKGSFESARINTFQQRYNSFLSEVAH
                     LSGLIPDSTATPESDSLASVLIHKRDSLISSFRTYTLSEVRANPYCLSSVPFILTTFD
                     GGQELLPYELFGNTYQKVDSCLGLAYPDKPLVQTFHQHIQMQEKKLAQLHKADRYSTG
                     DVLPAISFETVDSQAISIPGLWAKAILIEFWADWCPQSVSTADQLRILYEKHSNQGFK
                     IIRVGMGLEPEALYQRVKRDSIPWINVAIPDSEKDYWVDSIGVVYLPFNFLIDRSGRV
                     VAKNLFGDTLVRKLDDLLGANSGRLKPDVHPKTGITSKPEVKVNTITSNPETK"
     gene            15099..15884
                     /gene="rsmA"
                     /locus_tag="PK502_03030"
     CDS             15099..15884
                     /gene="rsmA"
                     /locus_tag="PK502_03030"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPG99822.1"
                     /translation="MPVVRPKKSLGQHFLHDRRIAGLIVSSLQAEGVDYVVEVGPGMG
                     VLTEDLLQRFGPVFYAVEVDGESVQYLRQRLPELGGNLIEADFLDVDIENCFNGRVAI
                     IGNLPYNISSQIFFKILAMRQRVVEAVVMVQREVAQRIAEKPGSRTYGILSVLLQAYF
                     NIEYLHTVSEGAFTPPPRVKSSVIRLTRNHVAELDCNPELFVRVVKASFNQRRKMLRN
                     AIHSAFPGVEAGKPFSERRAEQLSVADFVELTNMVEQQLTAVS"
     gene            complement(15886..17151)
                     /locus_tag="PK502_03035"
     CDS             complement(15886..17151)
                     /locus_tag="PK502_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG99823.1"
                     /translation="MNALKPFIILIRLARESMQFAYQSIKANKLRTFLSLFGITIGIF
                     AIISVFTMVDSLEKNIRNSLESLGNNVVYVQKWPWGGNEEYPWWKYLNRPEPTLNDMS
                     EIRRRSSLAQASCFNVSFNKQVKFQNKSLSNVTIYGTTNGYDEIRSFELGSGRFFNLF
                     ELNSGKPYAVIGATICEELFQNADPIGQSIQIGGKKATVIGVFAKEGKSAIGETSSDD
                     MVVIPLGMAKSMVDLRNSGPWIMVKARDNVVPEDLIDELRGILRSYHRLKPLDSDDFS
                     LNQIDMMKESLDAIFSSISFAGGFIAFFSIIVGGFGIANIMFVSVKERTSQIGIQKAL
                     GAKRYFILIQFLYEAMLLAILGGIIGLILIFAGTLIVTYGLDFTISLTLENIIWGLAI
                     SAIIGIASGFIPAWMASRLSPVEAINTKS"
     gene            17655..19181
                     /gene="purH"
                     /locus_tag="PK502_03040"
     CDS             17655..19181
                     /gene="purH"
                     /locus_tag="PK502_03040"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPG99824.1"
                     /translation="MEVIRQIRKALISVYHKENLEDIVRTLDSHNVLIYSTGGTLDFI
                     HSLGVKAIPVEELTGYPAILGGRVKTLHPKVFGAILARRENENDQFQLTEYAIPEIDL
                     VVVDLYPFEQTVVSGASEAEINEKIDIGGVSLIRAAAKNYRDVLVVSNRQQYARFSKM
                     LEENGCTTSLEQRKQFAAEAFETTSHYDVAIFNHFNNQFPQMAFKQSIQGVKELRYGE
                     NPHQKGIFYGSFDGMFEQLHGKEISFNNLMDIDAAVNLIAELDQPAIAILKHNNACGV
                     AVRDTLLQAWSDALAGDPVSAFGGVIVANRAISMDTAEAINKIFLEVLLAPDFEEDAL
                     ALLKSKKNRILLRYKDTRLPAITFRSLLNGVLVQERDSIKHDSAGLRIVTKVAPSTHQ
                     VEDMLFANVVVKHSKSNAIVLVKDKQLVGSGVGQTSRVDAVRQAIDKAKTFGFDLQGA
                     VLASDAYFPFPDNVELAAAAGIKAIIQPGGSVKDQESIDCCNRHGVAMVFTGIRHFKH
                     "
     gene            19221..20243
                     /locus_tag="PK502_03045"
     CDS             19221..20243
                     /locus_tag="PK502_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPG99825.1"
                     /translation="MGLFSFLTQELAIDLGTANTIILYNDKIMVDQPSIVAIDQSTGK
                     MIAIGETARAMHGKTHENIKTIRPLRDGVIADFNAAEQMIRGMISMINIKNLVFTPSL
                     RMVIGIPSGSTEVEIRAVRDSSEHAGGRDVYLIYEPMAAALGIGLDVLAPEGNMVVDI
                     GGGTTEIAVIALGGIVCNKSIRNAGDGFTSDIQAYMRHQHNVKIGERTAEDIKIKVGS
                     ALPDLENPPADLIVRGPNLMTALPIEVPVSYQEIAYCLDKSLSKIESAIFNVLELTPP
                     ELYADIVEKGIYLAGGGALLRGLDKRLTEKINIPFRVVEDPLHAVARGTSIALKNVGK
                     FPFLMR"
     gene            20433..21251
                     /gene="mreC"
                     /locus_tag="PK502_03050"
     CDS             20433..21251
                     /gene="mreC"
                     /locus_tag="PK502_03050"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPG99826.1"
                     /translation="MNSFIRLLQRFSYIILFAVLLAVSLSMYINTSYYQKAKVGAATG
                     VITGFFSEKLHNIRSYMDIKRDNVKLSLENVELRNEVERLREVIGKCADTAGYNLADT
                     SITLIRAMVVNNSVSHRQNFITINIGINDGAYQGMGVICDGGVVGVIAATSPNYSTVI
                     SLLNTTLKVSAKHAKSGVFGTLSWNGYNYQEVELTDIPLHVGISVGDTIATSGFSSIF
                     PKDIPIGVIKDFYKKDGSFYNIRVQLLTDFRALNYVYAVRVRNITEINQIEGSR"
     gene            21264..21758
                     /gene="mreD"
                     /locus_tag="PK502_03055"
     CDS             21264..21758
                     /gene="mreD"
                     /locus_tag="PK502_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPG99827.1"
                     /translation="MRYILIFILLLLIQLLVLNNINLGGYINPYVYLLFILLLPYGTP
                     RWVLLLVGFFTGLVVDYFSNTLGLHASATLILAFVRPSIMSGLRFSNNINERSMLNIS
                     LTGTEWFIRYAALGIVIHHSTLFMLETFSFRFFHLTLLRILLSSLVSFVFIFFLEVLL
                     TRKK"
     gene            21765..>23267
                     /locus_tag="PK502_03060"
     CDS             21765..>23267
                     /locus_tag="PK502_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPG99828.1"
                     /translation="MRFNFQADKRFTITGLIIIVFIIIAGKLFYIQVIDRSYQLSASS
                     NVLRYVTKYPARGLIFDRNGNLLVSNKVVYDLMMVKKQVVPFDTMAFASLLNITTDHV
                     RDAFVQASRQKGYSPRKSVVLLKQLPAEACAMLQEKLYLFPGFELLPRTVRLYDREVA
                     SHVLGYIGEVDEEIISKNSYYQLGDYIGISGLEKSYEEYLRGKKGVSVYMVDVYNRIK
                     GSYEDGKYDSAAVEGQNLICTLDADLQEYGEKLMQNKIGSIVAIEPRTGEVLAMVSSP
                     TYNPGLLVGRERADNFRTLQNDSLKPIFNRSIMALYPPGSTFKVVNALIGLQEGCVYP
                     QTTYSCHGGYTVGRGVGCHIHPSPLNLTQAIQMSCNTYFCHVFRNILDKPEFGSVEKG
                     FTVWKNHVESFGYGNKLNIDLPNEQKGIVPTVAFYDKYFRKGGWNSLTILSLSIGQGE
                     LAATPLQMANLVTIIANRGFYLDPHVVRAVGDSMVRNSNVPEKHYTTIDTQ"
BASE COUNT         5863 a         5152 c         5480 g         6772 t
ORIGIN      
        1 gcactataag cctagaagca tcatcatatg taaccagtga acgtggcttg ccggactata
       61 gtaaggataa cacctactgg ataaggtgga accatacaca ggatgctaag gcaaacccca
      121 actccacctt tcaggccaac gtcaactttg gctcatccaa ccataaccgg tacaatgccc
      181 gaaacataaa taatgccctg gcaaacactt tcaactccag catttcgtac tcaagaatat
      241 ggccgggttc acccttcagc atgtcggctt cgcttaacca ttcgcaaaat agcatagact
      301 agtctgttga tctaggtttt ccgaaagttt cgctcaacat gagccgtatt tacccattca
      361 agcgtaaatt gagcgttggg aaaccctcgt ggtacgaaaa gattgggttg agcctaaaca
      421 caaaccttga aaacagggtg caggtgaagg aggacaacct gtttaaaagt tcggtggttg
      481 acagcatgca aaacggcgtg aggcacgata tacctatttc aacctcattt aacctgctaa
      541 agtttataac cattagtcca agtgctagct acaacgagtt ctggtacttg caaacaatca
      601 acaagcactg ggatcccgat tcacaggtag ttgtggttga taccataaaa ggcttcaagc
      661 ggggatacca gtacagcacc gccgtgtcca tgagtacaaa gatttatggg acattccagt
      721 tcggaaagaa atcaaaggtg caggcaatca ggcatgtgat tacacccggt atttcgctga
      781 gctaccgtcc cgactttgcc aaggagagtt ttggattcta caagtctgtt caggtggatt
      841 caaccggaag gatggcacag tactccattt ttgaaagggg aatttacggt gggccgggag
      901 gtggaaaatc gggggtcgtg aacttttctt taggtaacat ggtcgaaatg aaggtgctct
      961 cgccaaagga tactgctaac ccggttaaaa aggtaaaaat tctggaaggg ttcagcatca
     1021 atacctccta taacatgctg gccgactctc tgaactggtc ggatatctcc gttagcggcc
     1081 gcaccaatct tttcaacaac atcaacatta acttttccgg tgcatttagt ccctatgcaa
     1141 ttgatgctaa gggaagcagg tacaacaggt ttgaacttga taggagcggc aagctggcgc
     1201 gttttaccag gggcagcgtt gggctagact ttgcgttgaa tagcagcaag ggtagcgcct
     1261 cttcaggtgg ggatcagtcc aggtcgcagg gaatgggtgg aagcaatccc cccttaggaa
     1321 ccgcacccga tggttcgtcg tttggtgagt ctctgggcac acagtacggc atggattatg
     1381 tcgattttag cgttccatgg aacctcaggg taagctacaa cctgagctac tccaaacctg
     1441 cctatgtatc ctccttagtt cagaccctga ccttttcggg cgatatcaac ctgactccaa
     1501 aatggaaaat tgcctttagc tccgggttcg atttgaagag ccggcagctt acaaccacgt
     1561 ccctcaattt ctacagagac ctgcactgct gggagatgcg gctgaccgtt atacctattg
     1621 gatactacaa aagcttcagc tttcagataa acgttcgctc ctccatactt caggatttaa
     1681 agcttaccaa gcgcgaccac tacctcgata acctgggcga ataaccgggc tgattttctt
     1741 gattgcttac tagcatgata aatttttgcc gaaatctata tttattacac taaaatttgt
     1801 taatcatgaa aaagattatc aacacaaatg atgctcctcg ggcaattggc ccatacagcc
     1861 aggctgttga ggccaatggg tttctttttg tgtcggggca ggttcccgtg aatccatcaa
     1921 ccggtaaaat tgtagaggga ggaattgctg agcaaaccga gcaggtagtt aaaaacatag
     1981 gggcaattct ggcgaaggcc ggccttacct atgccaacgt ggtgaaaact acctgcctgc
     2041 ttagcgatat ggagaatttt gccaccatga acgaggtata tgctcgttac tttacagctg
     2101 aaatgccggc aagggctgcc tttggggttg ttaaactccc gcttggagcc ttggtggaga
     2161 ttgagtgcat agccataagg tagaaaaaaa acctgccaac ggcaggtttt tttatgctgg
     2221 atgagtagtg ctattccgat actacatcaa attcgatgct gaccttcacc tccttatgca
     2281 gctttacctc ggcggtgtag gagccaacct ctttaatttg gtcgtcctga atgaagatta
     2341 gcttgcggtc aatttcaaat cctttctcgg caagcgcctc cgaaatctga atggtgttca
     2401 ccgaaccaaa aattttaccg gtcgagctgg ttttagctcc aatggtaagc ttcaggccgt
     2461 taagcttttt agccagctct tcggcatcgg ctttaatttt tgcctcctta tgggctcgct
     2521 gcttggtgtt ttcggccagc acttttttgg ccgattctgt ggcaatggtg gcaaagcctt
     2581 taggaataag atagtttcga gcgtaaccgt ttcgaacgtt tataatatcg tttttacttc
     2641 ccaaactggg catgtcctgt aatagtatga tttccatttg cttatctcct acctttaatt
     2701 atttcatcaa atcggttaca aatggcagta gggctaaatg ccgagcccgc ttaatggcag
     2761 tagatacctt tcgttggtac ttttgcgagg ttccggttaa ccgccgggga agaattttcc
     2821 cctgctcgtt taaaaatttt tttagaaatt ccggatcctt gtagtcaata tatttaatcc
     2881 tgtttttctt gaaacggcag tacttctttt tcttgacttc tacagttggg ggtgtgaggt
     2941 atcttatttc tgattgattc tggctcatgg cttaatcctc cattgtttta ggttccacct
     3001 tttctacctt gttgctgcgc tttttctcgg catactcaac agcatactta tcgagcttaa
     3061 cggtaaggaa acggataata cgctcgtcgc gacggtactg tgtctccagc ttatcaatca
     3121 gctcaccggg tgctttaaac tctaacaggt ggtaaaatcc ggttgttttt ttctgaatgg
     3181 ggtaggccaa ctttcttaat ccccagctct cttcatggat aatttcaccc ccatcttggg
     3241 tgatgaaatc cttgaattta gtgaccgttt ccttcatctg ggcctcagac aaaacgggag
     3301 tagcaatgaa aacggtttcg taatggttca ccataaaata tgattttagt taatagggtg
     3361 caaaattata gctaattttt aaatcttcca aatacacaga gggtagaatt ctcaagaata
     3421 aatagcatcg gcttaaggtg tttgcggttt gcaccctcat gttcagggaa aacttgttga
     3481 agcctaaacc tgtttatggc gcagtggagg ttggacaatt aaccttgtag cttctttttt
     3541 gctgagggga ggcaagggtg tgtgggctac aaattggaga acccattcag ggttgacttt
     3601 gctataattt ctaagcaccc atcctatggc cttacatata aaaaattcac tgctaggcag
     3661 caaggaatga attgtggcgg cgagaagtac ttcgtcggtt tgctgcttgt agttaagctg
     3721 gaagattagt gccgagcgtt taagccaaag gttgtccgag gtaacccatt tttgaatgaa
     3781 cggtttccgt tgttcaggaa atgaggcaaa gtactcgccc atgagccgtg aggcaatgta
     3841 gtcaaccgta tcccaccacg atttggtggt taccatgttc tcgtataggc ttatatcggc
     3901 tagctccagt tgtttatgga atttgtaggc catttcttgg gcaaagtatt gaaattcccg
     3961 ttggggttta agccagagcc tgttgatggt tgagccgagt tcgtttttag caggcaggtg
     4021 ttctggtgta aacaatagct tttgaatctc tcgtcggtca gccgactgaa tgccgaaaaa
     4081 ctcaaactga tttcgcatgt attttttttg ttgaagcgcc accgctggat tgcccttttg
     4141 taggaatttt acttccagct gctctacgag gttgtccatt tgcttggttg tagtttttcg
     4201 tattatattt aagctactgc tacaggatta ttatcaaggt tcggcccaat caaagcaagg
     4261 cttatccgca caacgtggat aaacaagtat ggggtgctga actgcatggt ttttgctatg
     4321 cccagcgtac caacgggaaa tccatgcgat ggggaagcat ggggttttta ggatatatgc
     4381 gtattccgca ttcaaaagca cccggctcta caggtatgag gttgagcctg taggtaaccc
     4441 tggagccttc aactgcttga acttgaaatt catggacccg tttaaccttt acttcgcctc
     4501 cttcaattag gtcggccagc accatctcaa ccccgatttc gtcgggacca agggaaccca
     4561 tgtccaggat aacctcggcg gtgtactctt tacccatgat aatggcctcg cgcgaaacat
     4621 caaactgttt gacctgaatg atttcaattt catcccagtt acgggtaatc cgtcgtttcc
     4681 atgacgaaat ttttttggct acctcaaagt cgttttctat aagctcggag gaacggaagt
     4741 agagcttatt gtaaaacctg ttctggtagt cgtttatcat cctaagggtg gtgaagttag
     4801 atgctacctg ggtcatggat ttacggatga atgaaatcca tttatccggt atatcgtcgc
     4861 tgttacgttg gtagaatgcg ggtacaattt cgttttccag aatggtgtat atcatttcgg
     4921 catcaagttc atcctgaaac tcggattcgg cgtaggtgct ctccatggga agtgcccatc
     4981 cggcattttc gcggtaacct tcaacccacc agccatccag gacactgaag tgaagcacgc
     5041 cgttcattac tgctttttgg ccgctggtac ccgaagcctc cattggtcgg gtaggggtgt
     5101 tgagccatac atcggtacct tgaatcatgt gcctggagag ctccatgtcg tagttttgca
     5161 ggaacagaat tcgtccaagg aagcgcggtt gcttggcaat ctcaatgatg ctttttatga
     5221 ggtcttgtcc tgccttatcg ttagggtgtg cttttccggc gaagaggaat tgaacaggat
     5281 gttcggggtt attcaccagc tggtccaacc ggtccaaatc tttaaaaagg aggttggcgc
     5341 gcttgtaggt tgcaaaacgg cgggcaaacc caatggtgag gatgctatcg ctaagttttt
     5401 cctgtatctc aattacctgc cgtggacttt cgaagcgtgt catttcgggg ttactcaacc
     5461 tgttgttgat aagtttgatg agccttttcc tgagggtatt acgaatttcc caaatttttg
     5521 aatctggaat attctccaac tcgttccagg caggagtttt gtaaccattg ctgttagtgg
     5581 cttggccgtt ttccataatt gatttccact cccgtgcggt ccatgttggg aaatggacgc
     5641 cattggtaac gtagctgata tgtagctcgt tggggaagta gcttggccac attccggcga
     5701 acatttttcg gctcacctca ccgtgaagcc agctaacccc atttacctcc tgggccaggt
     5761 ttgttgccag aacgctcatc gaaaatcttt gtgtggaatc accgggtacc atacgaccca
     5821 ggtccatgaa ctgatcccat gaaatcatca gcctgtcggg gtagtgggac atgtatgttc
     5881 gcacaagttc ttcggggaag gcatcatgac cggccggaac gggtgtatgg gtggtaaaca
     5941 ggctggtagc ccgtacaacc tccatggctt cggagaatga aagtttttca tcgagaataa
     6001 actttctaag cctttccagt cctgtaaatg ctgcatgacc ctcgttaagg tggtaaatgt
     6061 cggcctttat tcccagttca tttagcgctc tgattccgcc aattcccaga atcatttcct
     6121 gcataaacct gttttccagg tcgccaccgt aaaggtagtg agtaattgtc cggtcgttta
     6181 cgaggttctc ctcgaagtcg gtgtccaaca ggtaaagctc tgttctaccc acatcaacct
     6241 tccagattcg tgcctgcacc tgccttccag ggaatgccac gctaactgta acccatctgc
     6301 catcctcgtc gcgaactggt aaggccagcg attgggtgaa gttttgctgg tcgtatacgg
     6361 ctatttgctg gccagaggtg gatagctgct gggtaaagta accgtaacgg taaagtaggc
     6421 caacacctac catgtcaata ttcctgtcgg aggcttcttt gaggtagtcg ccggccaaga
     6481 tgcccaaccc acccgagtag agcttcagtg agctgtggag gccaaactcc atgctgaagt
     6541 aggctatttt aggccctttg cgctggtagc gctgcatcat gtaggcggta aaattcaggt
     6601 atatggagtg aagcttttcc acaaattcag catcgtgctc cagctcctga aaacgcggta
     6661 ggctaattct ctcgaggaat agtatggggt ttttctcaca ttttatccag agttccttgt
     6721 cgatggattc aaatagtttt ttggaatcgg tattccacga ccaccaaagg tttcgtgaaa
     6781 gctcatcaag gggttttagc ttttcaggaa ggttcttctg aacaatcagg cgggtccaag
     6841 agggttgggt ggttcggaat ttgcgctcaa ctgtaggcag aatttcctgc ctttcgagtt
     6901 ccatgaactg atgggttcgg ctaccaacca gcgaaagggc tttatcgtaa gctttatagt
     6961 agtagtcaat aaagtttttc caaagggcaa tagatgcaat ttcctgcgca ttgttcatca
     7021 gctcgttata gcgctcatgg gtgatccggg tgaaggcatc aatggtggag gcaatttgga
     7081 cagttacctc cagatcgttt tcgtcgttac gtttgatgat ggtaattgcg tctttaggct
     7141 ttttgtagtt gtttatcacc cagtcggcaa atcctgcaag tgaggtggtt atcgttggaa
     7201 ctttgaaagc taagctttcc atgggggtgt agccccaagg ctcgtagtag gaagggaaaa
     7261 cggtgaagtc catgccgatt agcaggtcgt agtagctttt gttgaatatt ccgtcgttac
     7321 cgttcaggta gcagggaacc caaaacacct ttaccttgtc ggatgatgag ttatttaagc
     7381 caagcgccct tattttttta agaattggtt cgctatcggg gtcgtttagg tagtgggtaa
     7441 cgtgagtatc gtcaaggatg ctatagttgc tatctttttc tttttcgagc aggttgtgaa
     7501 aaacatcctt tcgggggcca tgatgtccgg caggtataag caggaatgcc agcacctctc
     7561 tgccaaggtt attgtcccta ttcagcttgc ccagggcatc caaaaaaaca tctatcccct
     7621 tgtttttaaa ttcgtagcgt ccgcttatac caacaatcat gctatccttc gcaacttcgt
     7681 gcgccagcat ggcttctgcc acctcgtaaa atttaatttt accctcaact ctccgcagct
     7741 tgtgttcttc agcttcgggt ataaaggtat cgtcgaaacc gttaggggta agtaggtcaa
     7801 catccttttt aagaaagtgg ctgcattccc tggcggttat atcgctaaca gtggtgaagc
     7861 aatcggcttg ctgggcactg attctttcta acgattgctt ggagataaca ccaaattctc
     7921 tggccttgct ttcagggtca attttttcga tgttgctata cagcggtatg ttgttaccag
     7981 caatacttcg tcctagcacc gtagcatggg ttgtgaatac cgttcctact tgtggaacat
     8041 ttgcttttag gtagagcaat cctgttccgg tcatccactc gtgaaactgg gctactatgc
     8101 gatgtcgtaa gcttaggttg aagttggtaa aactttcaat caccttgcct gctgcatagc
     8161 caaatagggc gggttcaata tagtcccatt gtccggttat ggaatcgagc ttgaattttt
     8221 cccaaaagcc tttgaaaatc tcatcttttt ttgatataag ttgggaaaag ttgaccagaa
     8281 tagctaccgg tttacagggt atattccatc ggccaaccct tatgggcatt ccgttttcaa
     8341 tggcatgact acgccaggcg gcaaataggt tggtgtcttc tatgaattca gcattatgct
     8401 caatcccctt tattaggtcg gggccaacag taatgtagtt gttcttcagc tcattttcaa
     8461 tcatcgatgc ctttgttgac ataacggtgt gtatcccacc aaccttgttg cacacctccc
     8521 agcttacttc aaaaaggtaa tcgggtttta gcagcttgtc attcattcgt tcacaatatt
     8581 tttatggtag tattttgatt ttttccaagt ttaatccaaa ataaataggt taaccatttc
     8641 atttgttgct acaggtttgt cattatttgg cagtttctac cgattaacct actctttttt
     8701 cttttttgtt cccttagtgg ttgactttga attacttgat ttggtactgg atttggcagt
     8761 tgttgggcta cttttgatac tgttcttctt ttttgaagat ggctttagcg ctatttcttc
     8821 agttttggtg cttacaacag gcttgctttt aggcgtagtt ttactctttg gtttagcaac
     8881 agcctttgta ggcttgctgc ttgtttttgc cttattacct ggtgtgctgg cagcgtcagg
     8941 attatcaact ttagctttgc cagttttgcc ggtaggcttt gcttgttgct catttagctg
     9001 attctttagc tcgttaattt tatcctcata gagtttaatg agcttttggg ggttgtctag
     9061 ttcagctgat gattcatcga ccaatgtttt ttcgagtagc ttatccttaa ccctaagggt
     9121 aaagtcgctt aggatgttca tgtagttgat gaatgcctcg tagggtgaac cgtaggggtt
     9181 gaagtacttg tgcacatcgc catcgctaaa ccatttggtg cacatgtagt agaagtggtc
     9241 gctggtttgg aggtaaagcc agtccctttt gatctcgggg tcatcaattt tttcaacctt
     9301 atcggtaagc gcatatagtt ggttgaaagc gctatcctgc agttcgttgc ccaaccatgc
     9361 ggtaaggtct ctttcctcgt cggcccagga aatagggtag ggaacgtgaa gcagggcaac
     9421 cggttggtgt ttctctgcag cctccgatgg ggtaaggaat tcaaaattgg agtgaccgaa
     9481 aaccctagat ggcagggcac gaaggaaatc gaatattccg gtttcagccc actggtgttc
     9541 accaaaggtt tcataatcca taaaaaggtt aacaatttcc tccttgggat caatgttgtt
     9601 gagccaccaa acaaactttt cggtggttag cggccactcg ttccagtcgc ggttggaaaa
     9661 gcggaaggcg atgtcatcgc ttagctggaa atttttcagc agcagcttca acttaggatt
     9721 tatagcgttg gcatacacga agttggaact tttccatccc agaatatgct tggcgccttc
     9781 ggtgagcatg gttttaaacc ccaaatcggc aaccatggcg ccaatttcat cggaatatat
     9841 aagctcggtg tttctgaagg ttttgggctt gtatccaagg agtttctcaa ttttattctg
     9901 ttgggcttca acctgccggt agaattcttc cttgctcttg agcgaggaga gggagtggga
     9961 gtaggtctcg gccaaaaact ccacacttcc ggttttggcc aacctgcgaa agctgtccag
    10021 tacgtccggt gcgtattgct caaactggtc gagcgcagtc cccgaaatgg agtaggaaat
    10081 tttaaacctt gtgccaaact cgttgattag gtcgagcata agctggttgg tcggtaggta
    10141 gcacttttcg gctaccttcc gcatgatgga gcggttggca aaatcgtcga agtagctatg
    10201 gtttttccca atatcgaaga agcggtacct tcggagccta tatggttgat gcacctgaaa
    10261 gtagaagcaa attgttttca tcagctcaaa atttttaact ttatagtaat aagttgcggt
    10321 atctacactg ttgtttatga ccagctggat gattcatgcc tactggagta tatgttttca
    10381 agaatttttc ctgcttagca catcttgata tgcttcaatt accttgctgg cggcattatc
    10441 ccacttcaag ctattcacct cttcctgtcc gtgcctgata aaaaacgtag ataatgcatt
    10501 atagttcagc aggccataaa tgctatcggc gagtgcatca atatcccaga aatcaacttt
    10561 tatggcgtat ttaagcacct cagatacacc cgactgcttg gaaatgataa caggtacgct
    10621 ggaacgcatt gcttctaatg ggcttattcc gaaaggctct gaaacggagg gcatgacgta
    10681 aacgtcggag aggtggaaca tcctgttcac atccgggcct ttaagaaatc cggtaaagtg
    10741 aaatttgtgg gcaatgccaa gctgggccac ccgacgaatc atccggttta gcatatcacc
    10801 gcttccagcc ataacaaagc gtacgttatg agttcgcttg agcaccttgt tggcagcttc
    10861 aacgaagtag tcgggaccct tctgaaaagt taccctgccc aggaaggtaa ctatttgctc
    10921 ggagaccccg cgctcgaatt ccatgtcctg ctcatcggag aagtccacag cgttatgcac
    10981 tgtaaccacc ttatcttctg gtattccata gcgggtaatt actatattgc gggtatagtt
    11041 actcacggtg ataaccttgt cggcaaccat cattcccctt tgctccacat cgaatactgc
    11101 ctggttgatg ttctccccac tcctgtcaaa ttcggtggcg tgaacatgca ctaccagggg
    11161 tttgcccgaa accatcttgg cggcaatacc tgccggaaag gttagccagt cgtgcgagtg
    11221 gataacgtcg aacttttccc ttgcagccag gttggctgct acaacagcat accgggtaac
    11281 ctcctgcatc aggttggggt tgtattttcc agaaaactta aaccgcgttt ttaccttgcc
    11341 ggattcatac tcaacagttt ctgcctcgtt atgctcgata attttagcgt actcttcagg
    11401 gtcaacatag gggacaatgt tggagctgat ctctagatac ttaaggtgcc tccagaactc
    11461 ctccaggttc ttggcggagt aggccacatc aacatcttcg gcagcaacca acctcacagc
    11521 cgatttatcc tcatcgccgt aagcttttgg aacaacaaag atgacgtcaa ctttttgctt
    11581 ggtaagtcca cgcgttaagc caaagcaggc tgttcctaaa cctccggtta tatggggagg
    11641 gaattcccat ccgaacatta aaactctcat acgcgtttgg ttaatcgcta tacttctcaa
    11701 tcatttttac aattctgatt agctcggcaa cgctccatgc ctgcgatatg cagccattgg
    11761 gccgttgtgg tggatcgccg tcgtaaacct ctgccacgga gcaaattccg tgtagtgcaa
    11821 tatcttcctg cagcgaatca actatttcct gagccaatcc taaagcgcct ttaccctgaa
    11881 gtttaaattg tgcttcaacg taggctccta aaagccaagg ccacgctgtt ccctgatggt
    11941 aggccatatc gcgtgttggt tgatcgccct cataccttcc cttgtagttt gggttgttcg
    12001 gcgaaagggt tcttaatcct tttggggtgt aaagctcatg gcttacaacg ttgagcacgg
    12061 caattttttt ctcatcatca attggcgagt agggtaaaga aactgcaaaa agctggttgg
    12121 gcctgatgtc ccagttttgt ccattttcgt caacgtagtc ggccaggtag tttttctctg
    12181 gaatccagaa tagctcgttg aatgattttt ttacctgatc tgcaatgggt tgccattctt
    12241 tcacaaacct taaatcttta tttttggtag ccagttcaag ggtaaacatc agggcgttgt
    12301 accatagagc atttatttca accgggtagc caatgcgggg ggtaaccggt tttgcctgaa
    12361 taacggcatc catccatgtg atagcgtagc ccttttcacc ggcccaaatc aagccatttg
    12421 gctgcatttt aatactaaag ggtagccctt cgcgataacc tttcaggata gccttcattt
    12481 ttttgccata atcgtcccac acttcggtgt agttttgtgt ataactggcg tattgttgaa
    12541 tggcccagaa ataccaaagt ggtgcatcgg cggagttaaa cgcttcggaa ttgcctgttc
    12601 tgacgttggg gaataacccg ttttttagat cggtgcttag cgagtcgagc agtgctttgc
    12661 aggttttaac atcatcgagt ccaagggtta atccgggtag tgaaatgaac gagtcgcggc
    12721 tccagctgct aaaccacgga tagccggcaa taatatcgta gcgttttccc cgttggatga
    12781 taaactggcg cgctgtgttg gcaatgcagt tcatgaagct gtccttgggt ggtcgccggg
    12841 ttagctcgga gttgaattta cgcagtaggg caggggttga ctgttcgttg gtggcagccg
    12901 aaaaaattat actttctccc tttctgatgg aggtttcaaa gtatccgggc acaaataggt
    12961 cttcatgtcc ctcatatcct cttctgattt cctccatata ctccacattg tagtaccagt
    13021 cgggggatgc aacaaactca ttttgcctgt tcagctgcat gtagaggaac ggaaagccgg
    13081 tgtagagttt ataccttacg ccattgctgc attcttggta tttagtgtta gcccacatat
    13141 tcgccttgct gaggctatga acatttctga aagccatgaa tggctttatc cttaactttg
    13201 ttgggctatg agcatccagc agggtgtaac gtatcatcac ctgctcctca ttgtgaacaa
    13261 aaagcagctc tttttttagt attacaccgc ccacccggta aacgagcgtt ggggttggct
    13321 cgtactcaaa atcaacaatg tatttatgtc cacgtggttc gtaaattcct ggatagcggt
    13381 gaatgcccag gttgaatacc ttttcgtgct gcaccaccgt ttcatcaacg tttgaaagca
    13441 gcacgtgatt ttcaccatca aactcatcaa taggcaggat cattagccca tggtacttac
    13501 gggtgttgca gagtacaatc gtggtactta aatatccacc agccctgttg gtgctaagta
    13561 tttccctctc tagtgagtac tctagattta caagttgatc cttgtcgaat gctagatatt
    13621 tcatggcaaa ggttaatttt aatatattta ggttatgaaa atttttaggt tttattcctg
    13681 ctacaagtta tcaaaaaatc atattgaatc acccctttgg ggaaactttt tgctactttt
    13741 gaggagcaaa tttttgaatt tatcgaaaat ttcgtttgca cccagtgtta atttttataa
    13801 aagttttatg cacccttcaa aatattacca ttcaacaact ttttttaggt tcctttttgt
    13861 cctgccagct gcaattgcaa ttagctcctg cactaattcc tataaacgag caagcattag
    13921 ccttaaattg agtgacatag gagagcaatg ggtttatgtt gggggatgga acggattagg
    13981 cagcgatata accgattctt ttaaggtaag cacacgcgga aggggtaact tttctctaca
    14041 aaccacaaca cccgaaatgc ttgcactttc gttcgacagg gttcattttc caatcctgtt
    14101 atgtgttgaa ccaggttcaa atgtggaggt aaccggtact gcaagttcat accaggtgaa
    14161 gggttcattt gaaagcgcca ggataaacac ttttcagcaa cgctataata gttttttatc
    14221 tgaggtagct catctcagcg gcttaattcc agacagcaca gccactcccg agagcgactc
    14281 gttagcatcg gtacttattc ataaaagaga ctcgcttatt tccagcttta ggacatacac
    14341 cctttcagag gtgagggcta acccctactg cctgtcctca gttcccttca ttttaactac
    14401 ttttgacgga gggcaggagc ttctacctta cgaactcttc ggcaatactt accagaaagt
    14461 tgattcctgc ttgggcttag catatcccga taaacctctg gtgcaaactt ttcaccagca
    14521 tattcaaatg caggaaaaaa aacttgcaca attgcataag gctgataggt attccactgg
    14581 agatgttttg ccagctataa gttttgaaac tgttgatagc caggcgatat ctattcccgg
    14641 actttgggct aaagctattc ttattgagtt ttgggctgac tggtgcccac agtcggtgag
    14701 cacagccgac cagctgcgca tactctacga aaagcactcc aaccaagggt tcaaaatcat
    14761 tcgtgttggt atggggttag agcctgaggc tctctaccag agggttaaga gggactccat
    14821 cccgtggata aatgtggcca tacccgactc ggagaaagac tactgggttg attcgattgg
    14881 tgtcgtttac cttccattca acttccttat cgatcgatcc ggtagggtgg tggctaaaaa
    14941 tttatttggc gatacccttg tgcgcaagct ggatgatttg ctcggcgcga attctggtcg
    15001 gctaaagccc gatgttcatc caaaaaccgg gatcacttct aagcctgagg taaaggtaaa
    15061 cacaattact agcaatcccg aaactaaata gcaagttaat gcctgttgtt cggcctaaaa
    15121 agagtttggg gcagcatttc ctacacgatc gtaggattgc agggttgatt gtttcgagcc
    15181 ttcaggcgga aggtgttgac tacgttgttg aggtagggcc gggtatgggc gtgcttactg
    15241 aggacttgct ccagcgattc ggccctgttt tttatgcggt ggaggttgat ggcgagtcgg
    15301 tgcagtacct taggcagcgc ttaccggagt tgggcggcaa tcttatagaa gccgattttc
    15361 tggatgttga tattgaaaat tgcttcaatg gacgagttgc cattattggt aacctgccct
    15421 acaacatttc gtcccaaata ttttttaaaa ttcttgccat gcgccaaagg gtggtggagg
    15481 cagtggtgat ggtgcaacgc gaggtggcgc aacgcattgc cgaaaagccc ggttccagaa
    15541 cctacggcat tcttagcgtg ctgcttcagg catacttcaa cattgagtat ttgcatacag
    15601 tgagcgaggg cgcgtttacc ccaccaccca gggtgaaatc gtcggtcatc aggctaaccc
    15661 gtaaccatgt ggcagagcta gactgcaacc cggaactctt tgtacgggta gttaaagctt
    15721 cgtttaacca acgtcgaaag atgctgcgaa atgcgattca ctcagcgttt cccggggttg
    15781 aagctggtaa gcccttttcg gagaggaggg ctgaacagct ttcggtagcc gattttgttg
    15841 agttgaccaa catggtggag cagcagctca cagcggttag ttagcctacg attttgtgtt
    15901 gattgcctca accggactta acctggaggc catccacgca gggataaaac cagatgcaat
    15961 tcctataata gccgaaatgg caagccccca gatgatgttc tcgagtgtaa gcgatatggt
    16021 aaaatcaagc ccgtaggtaa ctatcagcgt tccagcaaat ataaggatta gtccaataat
    16081 gccacccaga attgccagaa gcatggcctc gtagaggaat tggatgagta tgaagtagcg
    16141 ctttgcccca agcgcctttt gaatgccaat ctggctggtt cgctccttaa ccgaaacaaa
    16201 catgatgttg gcaattccaa atccaccaac aattattgag aagaaggcta taaacccacc
    16261 agcaaagcta atggagctga aaattgcatc gaggctctcc ttcatcatgt ctatctggtt
    16321 tagagagaag tcgtcgctgt caagcggttt taggcgatgg tatgaccgga gtattcccct
    16381 caactcgtca atcaagtctt cggggacaac gttatcgcga gctttgacca ttatccatgg
    16441 gccagaattt cgcaggtcaa ccatagactt ggccatgcct agagggatta ccaccatatc
    16501 gtctgagctg gtttcgccaa tggccgattt gccttccttg gcaaaaacgc ctataaccgt
    16561 ggcttttttc ccaccaattt gtatgctttg accaatagga tcagcatttt ggaatagctc
    16621 ctcacaaatt gtagcaccta tcacagcgta tggcttgccg gagtttagct cgaaaaggtt
    16681 gaaaaacctg ccgctgccaa gctcaaacga tcttatctcg tcgtatccgt tggtagtccc
    16741 gtaaatagtt acgtttgaca ggcttttgtt ctgaaacttg acttgcttgt taaacgacac
    16801 gttgaaacag ctggcttgag ccaggctact ccggcgacga atttcgctca tgtcatttag
    16861 tgtgggctct ggtcggttta gatacttcca ccaggggtat tcctcattgc caccccacgg
    16921 ccacttttgt acgtaaacca cgttatttcc taacgattcc aagctgttcc ggatattttt
    16981 ttccagactg tcaaccatag taaataccga gatgatggca aaaattccaa tggttatgcc
    17041 aaacagcgag aggaatgtcc gtagtttgtt ggcctttatg gactggtagg caaactgcat
    17101 gctttcccgg gcaagcctga tgagtatgat gaagggtttt agggcattca ttctgataca
    17161 tgttaaacaa agcaaaggta tttaaaaaat tatggtctag ccaaatggat ttgttaatta
    17221 ctctgaatgc gctggtggaa acagaatttt tgaaggagta tatttgttta accactatga
    17281 attctggcgt acacttgctt ttaataattt tatccgtaca aaatgtttag gttttctatt
    17341 acaaaaacct tggcccatac cttttaagtt tggggttgag gggatggatc tcccatattt
    17401 tggcttcgcc gaactcgcaa ctaacaaccg attgttgatg cgtgctcacg ctggctgcac
    17461 aacatgggtg ttttataaag ttttacaact tacttttttt attactctta acctgttact
    17521 cttaccccaa taatccccta aatttttgtt tatatatttt ttgctgttca tcattgaatt
    17581 ctcaaaggat ttaaccattt ttaccactcc aaatatttgg ggcttttaat ttgtatcagt
    17641 aggaaaatca ggcaatggaa gtcataagac aaataaggaa ggccttaatt tctgtttacc
    17701 ataaggaaaa cctggaagat attgtaagga cacttgatag ccacaacgtt ttaatttact
    17761 caacaggtgg aaccttagat tttatccaca gcctgggggt aaaagccatt ccagtagaag
    17821 aacttacagg atatccagcc atactcggag ggcgcgttaa aacccttcat ccaaaggttt
    17881 ttggtgcaat actggcacgt cgcgaaaatg agaatgacca gttccagcta accgagtatg
    17941 caatccccga aattgacctg gtagtagttg acctgtaccc gtttgaacaa acggtagtct
    18001 ctggtgcctc ggaggctgaa attaatgaaa aaattgatat tgggggtgtt tcgttgattc
    18061 gggcagctgc taagaattac cgggatgttc tggttgtttc aaaccgtcaa cagtatgcca
    18121 ggtttagtaa aatgcttgag gaaaatggat gcactactag cctggagcag cgtaagcagt
    18181 ttgctgctga agcatttgaa actacatcac actatgatgt tgccattttc aaccatttca
    18241 ataaccagtt tccgcaaatg gctttcaagc agagcattca aggggtgaaa gagttacgct
    18301 acggtgaaaa tccccatcaa aagggaattt tttatggtag tttcgacggg atgtttgaac
    18361 aactccatgg caaggaaata tccttcaaca acctaatgga tattgatgct gcagtaaacc
    18421 tgattgcaga gctcgatcaa cctgccatcg ctattttgaa gcacaacaac gcatgtggag
    18481 tagccgtgcg tgataccctt ttgcaggcat ggagcgatgc gctggccggc gatccggttt
    18541 cggcttttgg cggagtgatt gtggccaacc gtgcaatcag catggatacg gcagaagcaa
    18601 ttaataaaat atttctggag gttctgcttg cccccgattt tgaggaagat gcacttgccc
    18661 tactaaagtc aaagaaaaac aggatactct tgaggtataa ggacacccgg cttccagcaa
    18721 taacatttcg ctcgttgctg aatggcgtgt tggttcagga gcgtgattcc ataaagcatg
    18781 atagcgctgg ccttcggata gtaacaaaag ttgctccaag cacccatcag gttgaggaca
    18841 tgctttttgc caacgttgtg gtgaagcact cgaagtccaa cgccattgtg ctggttaaag
    18901 ataaacagct ggttggaagc ggcgttggtc aaacttcaag ggttgacgcc gttaggcaag
    18961 ctattgataa ggcaaagacc tttggctttg accttcaggg agcagttctg gcgtcggatg
    19021 cctatttccc ttttcccgac aacgttgagc tagctgctgc tgctggtatt aaggctatta
    19081 ttcagccagg tggatccgta aaagatcagg agagcataga ttgctgcaat aggcatggag
    19141 ttgcaatggt ttttacaggc ataagacatt ttaaacacta agcaataaac actaagcaat
    19201 aatataacta gacatagaat atgggacttt tttctttttt aacccaggag cttgccatag
    19261 acctgggaac cgctaacacc atcatccttt acaacgataa aattatggtg gatcaaccct
    19321 ccattgtggc tattgaccaa agcaccggca agatgatagc cattggagag actgccaggg
    19381 caatgcatgg taaaacccat gagaacatta aaaccatccg accgcttcgt gatggcgtga
    19441 ttgccgattt taacgctgcc gaacagatga ttcggggtat gataagcatg ataaacataa
    19501 aaaacctggt ttttaccccc tctctcagaa tggtaatagg tattccaagc ggcagcaccg
    19561 aagtagaaat tcgtgctgtt agggattcct cggagcatgc aggcggtagg gatgtttacc
    19621 ttatctatga acccatggct gccgccctgg gtattggcct tgatgtgctt gctcccgaag
    19681 ggaacatggt ggtggacata ggtgggggaa caaccgagat tgccgttatt gccctgggtg
    19741 gtattgtttg caacaaatcc attcggaatg ccggtgacgg ttttacatcc gacatacaag
    19801 cctacatgag gcaccagcat aatgttaaaa ttggcgaacg cactgccgag gatattaaaa
    19861 taaaggtcgg ttctgcactt cccgacttgg agaatcctcc tgctgacctg attgtccgag
    19921 gccccaacct tatgactgcc ctgcctattg aggttccggt ttcgtatcag gaaattgcct
    19981 actgtctgga caaatcgctt tcaaaaattg aatcggcaat attcaacgtg cttgagctaa
    20041 cacctcctga actttacgcc gatattgttg agaagggaat ttacctggca ggtggaggtg
    20101 cgctgcttag aggcctcgat aaaaggctta ccgaaaaaat taacataccc tttagggttg
    20161 tggaggaccc gcttcatgct gtagctaggg gtaccagcat tgcactgaaa aatgttggca
    20221 aattcccatt cctgatgcgt taaagggtaa cagagtttaa aaatcattaa gccccgtgtg
    20281 gctccgttag gaaaaatatt tctatatcca gctcgtctgg atggctacac aaaaaaatga
    20341 agcacctgat agcacccttg taaatggttg ttggagcgtt ttttaatatt ttcaacctga
    20401 tgttttgacg ctggtaatta agagtatcga ggatgaacag ctttattcgg ctacttcaac
    20461 gattttcgta tatcattctt tttgcggtgc tactggccgt ttcactttca atgtacatca
    20521 atacttccta ctatcagaag gcaaaagttg gtgcggcaac cggggttatt acaggtttct
    20581 tctctgagaa gctccacaat attcgtagct acatggatat taaaagggat aatgttaagc
    20641 tatcgcttga gaatgttgag cttcgaaacg aggttgaaag acttagagaa gtcatcggga
    20701 aatgtgccga cacggcaggc tacaaccttg ccgacaccag cattaccctg attagggcaa
    20761 tggttgtgaa caactccgta tcccaccgtc agaactttat aacaattaac attggtataa
    20821 acgatggcgc ttatcagggc atgggtgtga tttgcgatgg aggagtcgta ggggttatag
    20881 cggcgacatc gcccaactac agcacggtta tatcgctgct taatacaacc cttaaggttt
    20941 ctgccaagca tgccaagtcg ggtgtttttg gtaccctgtc atggaatggt tataattacc
    21001 aagaggttga gctaaccgat atacccctgc atgttggcat ttctgttggc gataccattg
    21061 ccactagcgg tttctcatcc atttttccaa aggatatacc cattggtgtt atcaaggatt
    21121 tttacaagaa ggatggtagc ttctacaaca ttcgtgttca gctgcttacc gattttcggg
    21181 cgcttaacta cgtgtacgca gttagggtta ggaacattac ggagattaac caaattgagg
    21241 ggagtaggtg atacaaggat tacttgcgat acattctgat ctttatcctg ttgcttctga
    21301 ttcagctcct ggtacttaat aatattaacc tgggagggta tatcaaccct tacgtttacc
    21361 tgttattcat acttttgcta ccctatggca ctccccgctg ggtcttgctg cttgtaggct
    21421 tttttacggg attggttgtc gattactttt caaataccct tggacttcac gcttcggcta
    21481 cgctaatact tgcttttgtc cgcccgtcga taatgtctgg tctaaggttt agcaataaca
    21541 tcaacgagcg gagcatgctc aacataagcc ttacaggcac cgagtggttt attcgctatg
    21601 cagcactggg aatagttatt catcattcta ccctattcat gcttgaaacc ttcagcttcc
    21661 gcttttttca cctcacccta ctgagaatcc tacttagcag cctggtttcg tttgtgttta
    21721 ttttcttcct tgaagtttta ctcacccgaa aaaagtaatg ggctatgagg ttcaattttc
    21781 aggctgataa acgattcacc attaccgggc taatcatcat agtgtttatc atcatagcgg
    21841 ggaagctatt ctatattcag gtgatagaca ggtcgtacca gctttcggca agcagtaacg
    21901 tgttaaggta tgtaaccaag tatccggctc gtgggttgat attcgaccgg aacggaaacc
    21961 tgctggtttc gaacaaggtg gtttacgacc tgatgatggt gaagaagcag gttgtaccct
    22021 ttgatacgat ggcatttgcc tccctgttga acattactac cgaccatgta agggatgcgt
    22081 ttgtacaggc ttcccgccaa aaagggtact ccccgcgcaa atcggttgtg ctgcttaagc
    22141 agcttccggc cgaggcatgt gcaatgcttc aggaaaagct ttacctgttt ccgggatttg
    22201 agctgctgcc acgtactgtt aggctttacg acagggaagt agcaagtcat gttctgggat
    22261 acattggcga ggttgacgag gaaattattt caaaaaattc ctactaccag ctgggcgatt
    22321 atattggtat cagcggtttg gaaaaatcgt atgaggagta cctccgtggg aaaaagggtg
    22381 ttagcgttta catggttgac gtttacaacc gcatcaaggg ctcgtatgag gatggaaaat
    22441 acgattctgc tgccgtagag gggcagaatc taatttgcac tttggatgcc gaccttcagg
    22501 agtatggcga aaagttgatg cagaataaga taggcagcat tgtggctatt gagcccagaa
    22561 caggcgaggt gctggccatg gtttcaagcc ctacgtataa tccaggatta cttgttggcc
    22621 gcgagcgtgc cgataacttc aggaccctcc aaaacgattc gctcaaacca atttttaacc
    22681 gctccatcat ggccctttac ccaccgggtt ctacctttaa ggttgtgaat gcgcttattg
    22741 ggctacagga aggttgcgtt tacccgcaaa ccacctatag ctgccatgga ggatacaccg
    22801 ttgggcgagg tgtgggatgc cacattcacc cctctccatt gaatctaact caggccatcc
    22861 agatgtcgtg taacacctat ttctgccatg tgttccggaa catactcgat aagcccgaat
    22921 ttggatcagt cgaaaagggt tttacggttt ggaaaaatca tgtcgaatca tttggttacg
    22981 gtaataagct gaatattgat ttacccaacg agcagaaagg gattgttccg acagttgcgt
    23041 tctacgacaa gtactttaga aaaggcggct ggaattccct taccatactc tcgctttcca
    23101 ttgggcaggg agagctggca gcaacaccct tgcaaatggc aaacctggta accattattg
    23161 ccaacagggg gttttacctt gatccccatg tggtgagagc tgtaggtgac agcatggtga
    23221 gaaactcaaa tgttcccgaa aagcactaca ccacaataga cacccag
//
LOCUS       DAOPHX010000023        23243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_2292_length_23243_cov_6.258927, whole genome shotgun sequence.
ACCESSION   DAOPHX010000023 DAOPHX010000000
VERSION     DAOPHX010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 23243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23243
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2292_length_23243_cov_6.258927"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PK502_03065"
     CDS             complement(<1..985)
                     /locus_tag="PK502_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPG99829.1"
                     /translation="MAKKSKKIFVLDTNVILHDYHCIYNFQENDIVIPIVALEELDYF
                     KKGNEMVNYNAREFVRELDKITGDNLFNGGVSLGKNLGKLRIETGKPMPKAMLDSFAE
                     ATPDHRILAITYHLASVNPDRPVILVTKDINLRMKAKSLGIVAQDYLTDKVQDIDSLK
                     KEIEIIDGVDDEFIQKLYENPSELGAAPLKVKPYANQYLILKGNKSSALAHFDKATNM
                     LNRVEKTRAYGIEPRNSEQTFALDALLRSEVKLVALTGKAGTGKTLLALAAALQQEEN
                     FNQILLARPIVALSNKDIGFLPGDINEKISPYMQPLFDNLSVIKNRFKPASK"
     gene            1382..1879
                     /locus_tag="PK502_03070"
     CDS             1382..1879
                     /locus_tag="PK502_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPG99830.1"
                     /translation="MSQQEFNTALVELEPNLERFAYSLTANREDANDLLQETYLKALT
                     YKDKFEDNTNLKAWTFTIMKNTFINNYRKAVKQNTTFDSSENQFLLNSSYEKNGPESM
                     YSHSEISKKIDQLEDEFRIPFQMHTSGYKYKEIAEKLNLKIGTVKSRIFFSRQKLMSA
                     LRDYE"
     gene            2069..2142
                     /locus_tag="PK502_03075"
     tRNA            2069..2142
                     /locus_tag="PK502_03075"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2103..2105,aa:Arg,seq:ccg)
     gene            2412..5411
                     /gene="secDF"
                     /locus_tag="PK502_03080"
     CDS             2412..5411
                     /gene="secDF"
                     /locus_tag="PK502_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HPG99831.1"
                     /translation="MQNKGAIRFIAIALILVCIYQLSFTLVTRRVEKKAREYAMVGNE
                     IDRAKETHYMDSISSEPVYNFLYIRKYTYKECKEREINLGLDLKGGMNVMLEVSVTDL
                     IRSLSNNSQDSSFLKAMEVAQKMPGSEHFVTKFGKAFQQVAPNARLAAIFSTPELRDK
                     INFESTNDQVIKILRAEADNAINNSFNILRNRIDKFGVAQPNIQRLENSGLILIELPG
                     IKEPERVRKLLQGTANLEFWETYEFSELFNNFIAANNALAEIQDATAKTDSIKSTSNK
                     ELTAKKDTVTDELLAKLKATSTEDSLSATQADQTKKYPLFSLLQPNLYQDQPAPGPLV
                     GYAHVKDTARVNEILRMPKIKALFPVDVKFLWGVKPPKWDKSESLYELIALKVTSRDG
                     KAPLDGGVITDAREAFGETKGSAEVDMSMNAEGARVWARMTGENIGRCIAIVLDNYVY
                     SYPRVQSEIKGGRSQITGDFTINEAKDLANILKSGKMPAPARIVQEELVGPSLGTESI
                     NSGMNSFILSFILVLLFMMVWYSFKAGLVADIALFFNLFFIMGIMASFGATLTLSGIA
                     GIVLTLGMAVDTNVLIFERIREELRSGKGTIPSVNEGYRNALSAIIDGNVTTLLTGII
                     LYVFGTGPIRGFATTLIIGIITSLFTGILISRLIQTWMINHNINIRYSVKFTEHAFRN
                     LHIDFIGIRKYAYIFSGSLFLIIFISLGVRGLNQGIDFTGGRSYVVRFDDAVSTTDIA
                     SDLKHVFNEAPEVKTFGGQNQVKITTKFKIDSKDENIENEMDSLLYVGLKPYLQDGTT
                     LKDFQTTYKQSSIKVGPTIAQDIKRDAVIAVILALIVIFLYVLIRFRWWSYSTGAIVS
                     LFHDTMMIVGMYSLLYFRVPFGLEADQSFIAALLTIIGYSINDTVVIFDRIREYNKLY
                     PKRSSRDNINNAINDTLSRTFVTSFTVLIVLVPMFFLGGEVIRGFIFALIIGTISGSY
                     STVFIATPIAYDLEKAVEKRKNRKIKQ"
     gene            5656..6645
                     /gene="trpS"
                     /locus_tag="PK502_03085"
     CDS             5656..6645
                     /gene="trpS"
                     /locus_tag="PK502_03085"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPG99832.1"
                     /translation="MEIVLSGIRSTGKLHLGNYYGALLNFVQMQHEHKCFFFIADYHS
                     LTTHPTPTDLHGSVKQVLAEYLACGLDPEIATLYVQSDLPQVPELYLLLNMNAYVGEL
                     ERCTSFKEKIRKHPDNINAGLLTYPTLMAADILIHKATMVPVGKDQEQHLEMTRTFAN
                     RFNRLYKVDYFPEPVAYNFGKDLVKIPGLDGSGKMGKSEGNAIYLSDDPKTIRSKVMK
                     AVTDSGPTQPNQEKSEPVKNLFTLMRVVSDPETYSYFEEQYSSCHIRYGDMKKQLAED
                     IIRITTPIRERIEEILANDAYLSQVAHHGAEKARESASKTINEVRQIIGFRNF"
     gene            6869..7981
                     /locus_tag="PK502_03090"
     CDS             6869..7981
                     /locus_tag="PK502_03090"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPG99833.1"
                     /translation="MELEQVIQLLLSQFDFVSLPGLGSFVKRRNPAKVSDDRSSIEPP
                     SEYYIFDTSRTFDDEAITNYLCSTLGIDHKEAELRVTQLVERVNNALSSRKPFEFPGV
                     GTLLKDDNNNITLEFDASAAATESYGLSAIEVLPKQKADSKLTSPKENKNAAVAETQA
                     SSPLSTEQTTKIPTPKPAVAKKSRVRLWVPITACIVVVAVAVCWFTPALHFWEQSQTS
                     VSPSTTDSSSSMASVGNLPLASAIPDSIVEEEDSQVPDSISASGQPNATDEGGAEKIL
                     EQTTSKKVALSYTEPPVVETGKSFYIIAGSFTSEVNAQKFLSELAEKGYKPTLIPRNK
                     LYSVSLYTFSNRDRALRELERLKSEQTFEKLWLLSQ"
     gene            complement(8064..10118)
                     /gene="metG"
                     /locus_tag="PK502_03095"
     CDS             complement(8064..10118)
                     /gene="metG"
                     /locus_tag="PK502_03095"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPG99834.1"
                     /translation="MYKPFHPMSNYKRYLITSALPYANGPVHIGHLAGVYIPSDIYTR
                     YLRAKGADVISVCGSDEHGVPITIKARHENTTPQAVVDKYHAIIKESFKQFGIDFDIY
                     SRTTSPVHYETASEFFKKLYEKGVFVEKVTDQYYDEEVGQFLADRYITGTCPHCGNPN
                     AYGDQCEKCGTSLNATDLIDPRSTLSGSKPILKATRHWFLPLDKFQPELEKWILEDHK
                     EWKANVYGQCKSWLDQGLQPRAVSRDLDWGVPVPLDGAEGKVLYVWFDAPIGYISATK
                     ELTPNWEKYWKDSDTRLIHFIGKDNIVFHCIIFPSMLKAYGDYILPDNVPANEFLNLE
                     GDKISTSRNWAVWLNEYLVEFPGKQDVLRYVLTANMPETKDNDFTWKDFQTRNNSELV
                     AILGNFVNRAMVLTHKYFDGKLPARNALTDYDNSVLHELPRIKERLEHNLEEFKFREA
                     LKEAMNIARLGNKYLADTEPWKLMEKDPRQVANILNIAMQITANLSIILEPFLPFTAE
                     KIRKMINFQSKEWKLAGLDSLLAPGHILDKPQLLFDKIEDDVIAVQLNKLQATKLVNQ
                     NSVKNVPEQKGSISYDDFSKMDIRVATVLDAERVPKTQKLLKLKVDTGIDVRTLVSGI
                     AEYFTPEEMVGRQVLVLANLEPRKIRGIDSHGMILMAEDTSGKLQLVSPAAKVSNGST
                     VK"
     gene            complement(10275..10793)
                     /locus_tag="PK502_03100"
     CDS             complement(10275..10793)
                     /locus_tag="PK502_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPG99835.1"
                     /translation="MAIIKPLRGFTPKIGENCFIAETAVIIGDVEIGDDCSIWYGVVV
                     RGDVNSIRIGNRVNIQDNAVLHTTYQKTTIRLGNDVSVGHGAIVHGAEVRDGALLGMG
                     CTVMDNAVIGEGAIVAANALVLENTTVEPGTIYGGVPAKFIKKVDAEVSREMNERIAR
                     NYLFYAGWYKQG"
     gene            10928..11686
                     /locus_tag="PK502_03105"
     CDS             10928..11686
                     /locus_tag="PK502_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HPG99836.1"
                     /translation="MVKKFSKHIELRFYGGLNHFLPASKRQVPFNLPYMGTPTVKDLI
                     ESVGVPHVEVFLIVADSMSVGFDYKPEPSSQISCYPRFYNLSIDYGILRPTEPAPPVF
                     ILDVHLGRLASYLRLYGFDTLYSNCYSDDQILRLAAAENRIILTRDKGILRSGAAIHG
                     YYVRSTQPKEQLNEVVDYFSMACFADPFSRCSLCNGLVETVDRENVKGLVAENSYNHY
                     HSFYRCSRCGQIYWEGSQYKGILNLFGRHGPHHE"
     gene            11883..13736
                     /locus_tag="PK502_03110"
     CDS             11883..13736
                     /locus_tag="PK502_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPG99837.1"
                     /translation="MEKQTGVQELDEVAVRFSGDSGDGMQLTGTLFADASALFGNDVS
                     TFPDYPAEIRAPQGTIGGVSGFQVHIGSKKVNTPGDYCDVLVAMNPAALKANIKWVKK
                     GGMVIADADQFNEKGYQRAGYKTLDAVTELNLQDYKVIMAPITTLTKESLKDSGLDNK
                     SIVRSKNMFALGMVYYIFDRPMNFTETYFETKFKKKPEIIEANKKVLQDGYNYAANIQ
                     AIPNTYSIKPSKILPKGKYRTVSGNTATAWGLIAAAEKAGLPLFCGSYPITPATDILA
                     ELSKRKDLGVKTLQAEDEIAGICTAIGASFAGNFAVTSTSGPGLSLKSEALGLAVMTE
                     LPLVVVDVQRGGPSTGLPTKTEQADLMQALWGRNGEAPVVVISATSPSNCFDAAFNAG
                     KIAMEHMTPVILLTDGFIANGSEPWLIPNMKEYPKINPPLVEEGTQGYMPYARDDKRL
                     ARGWALPGKKGLTHRVGGLEKDFLKGSLSHDPINHQRMVDIRAAKVERVREFIPEQKV
                     IGDSEGDLLVIGWGGTYGHLLDAVTDLRGKGKSISLCHFSYINPLPKNTRDILSRYKK
                     LVVCELNMGQFANYLRMNFQEFNYLQFNKVQGLPFTVVELVEKFNQLLEGK"
     gene            13739..14770
                     /locus_tag="PK502_03115"
     CDS             13739..14770
                     /locus_tag="PK502_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPG99838.1"
                     /translation="MEEQYLNYTPQDFKSDQEVRWCPGCGDHAILSAVQKALPEVSKE
                     LKYNIERYVFVSGIGCSSRFPYYVNTYGFHGIHGRASAIATGVKVANPTLSVWQITGD
                     GDALAIGGNHFIHAIRRNIDVNILLFNNQIYGLTKGQYSPTSPLGKITKTSPFGTVEH
                     PFRPGELVIGAQGKFFARTLDVDVKLTSEIMVEAAKHDGTSVVEILQNCVIFNDGAYD
                     FISSKETRDDRVIVLHHGEKMVFGKNRDKGLVLSGLSLKVVTIGQGGFTMDDILVHDA
                     YNPNPGVHMMLANMEFPEYPIALGVIRNVKDSTYDDNVRDQVLEVMKDAKIKCVDDLL
                     NSGETWEVK"
     gene            complement(14899..18960)
                     /locus_tag="PK502_03120"
     CDS             complement(14899..18960)
                     /locus_tag="PK502_03120"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPG99839.1"
                     /translation="MKRILSIFTAVILLSSAVFAQQKVIPLKGSKSAISVKQSTLQQL
                     RVSVSFSSLISNEVVTESGEKYVNLAIPDGFSTGLVGTPKLPAYKKLIQVPLGAKPIV
                     KVHSFSHVDIALGDYGINHPVIPVQPPVRKDQNLDSLKFIMKAEVYRKNEMIEPQLVK
                     VDMLGTMRGVQVARLEVNPVAYNPVTGMLRIYNDIDVEVDFEGVSQAKFDEMLAKTSS
                     PYFETSYSFLINDLTKSSFDDHPDLTTYPVKMLIVSNRMFESTLVPFVEWKTRKGFNV
                     EVAYTDVIGNTAEAIKTFVHSSYTNATPENPAPTFLILVGDVAQVPASGVGSASYKQT
                     DLYYASVDGDYFPEMYYGRMSASTNVQLQNIIDKILYYEQYGFEDPTYLNSATLIAGA
                     DANWNPKVGQPTIKYGTKYYFNNAQGYSSIYEYGVSNDPSNPGAQPGYNGCYGSDQVA
                     VGFINYTAHCSETSWADPTLTSSTVEAMTNLNRYPLAVGNCCLSGDFATDECIGEKWI
                     RAAGKGAVTYIGSSPSTYWFEDFYWSVGAFPMVGENDGYVPTFEETTLGAYDAPHHGN
                     YVTTGGMVFAGNLAVTEVDAQGYPQQSSPHYYWEAYNILGDPSLVPYFTEASPNAVSH
                     TDSFPIGLSFYTVSALPGSYVAISKDGLLHGAALVDASGEVDVPVNPVLEGGDVDIVV
                     TRPQTIPYMARIPAAALDGPYITLDLYTIEDDNAKADFGEKFTIAVTLKNVGVDIANT
                     VSARLSGVDRYVTITDGDSVYFGDIPSGETGNTSTVNAAFELAVADSIPDRYVATFNL
                     DATDGTSTWPSKLWITVNSPKLSVKDFTLVGDDNSNGQLDPGENGQLRVLLSNQGHAD
                     AGDALVILSDDSPFLNINNPSKIVSLPLNSDLPVDFDVSASSGTFVGTEVNVTAAVSK
                     GGYTAEGNYHFGIGDKPEVRIGTGNLAAEGTYPFDSYYKANRSQILFKKEEIAANPKT
                     IKSIGFYFMQVGEISPFSNLKIKLLETELSYLGASYVNTDSATLVLNAASYTMPGTAG
                     WHVFDIEDFRYTATGNLLVEISFGLNSTWENDAYEVACSSTPFTSVVYGYDDDNPMPG
                     YDDNSSLRPNIYLQFGLSDFPNHSVSGLIFSNDLPVAIPGANISMQGFLTYNATTNED
                     GRFSIDSLLVDSYVLNVSGKGFVSYSDTLTVDGGMMLDTIKLSSASYPVTFSVYANGE
                     AVEGAEIAIEGYPVIYTNSLGVALTSLKDGDYSYNVTKTGYNPILGNTFTISGGDKNI
                     DILLFPLSAGDSKLNAAAAYPNPFDSYLRIGGAAEVGYIKLTSLTGQVVMEFRGNGSE
                     TYTLNTANLPAGFYLITMESASGEKLVKKVIKQ"
     gene            19194..20384
                     /locus_tag="PK502_03125"
     CDS             19194..20384
                     /locus_tag="PK502_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HPG99840.1"
                     /translation="MAAGFILFDDDRREALLPFTFTRPVSEIRIGILTITQKWERYLG
                     SSTSFLTTGYLSKKYPQNTTGERFCLINGGVCPDKALVNAIMSLEAGEVLVHENVVLA
                     GVMDKLPAETPSLSDFRVRIPYPNEVLQVKHLWDIFRLNGEALTRDFDMLTLNRKSAP
                     ISSSNRTLNPSRIFIEEGARVECSTLNAEAGPIYIASDAEVMEGALIRGPFSLGEHSA
                     VKMGAKIYGPTTIGPHCKVGGEINNSVIFGYSNKAHDGFLGNSVIGEWCNIGADTNNS
                     NLKNNYAEVKLWSYADNAFTSTGLQFCGLFMGDHSKCGINTMFNTGTVVGVCANIYGS
                     GFPRNFIPCFSWGGACGFKTHQPEKALETARLMMQRRNLELTQTDIDILKKIFDLTSQ
                     YRNF"
     gene            20488..22962
                     /gene="lon"
                     /locus_tag="PK502_03130"
     CDS             20488..22962
                     /gene="lon"
                     /locus_tag="PK502_03130"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPG99841.1"
                     /translation="MERNSRNFYSKLLIDDSNESNVNFIPILADDGTPELKDADIPDV
                     LPIMALRDTVLFPGIVMPITVGREKSLKLIKSLPPRKRMIGVVAQVDSKIPDPTAEDL
                     WHIGTVAQVIKVLEMPDRNFSVILKGICRFEVKNYLSEEPFFMARVSTLADILPDESD
                     KDFAAIASSIRDITIRIINLTPTLPQEAAFAIKNLDDNQLLVNFVSANSDISPEEKQK
                     LLEKSMLKERATQLLQYLTREVQMLELKQDIQSKVKLDLEQQQREYFLQQQIKTLQEE
                     LGANPTEQEVNDLKERAKQKKWSKEIAEHFDRELSKLNRMNPMAAEYSVQLNYVELLL
                     DLPWNEFTQDNFDLAHAREILDEDHFGLEQVKERILEHLAVLKLKGDLKAPILCLYGP
                     PGVGKTSLGKSVARALGRKYVRISLGGMHDEAEIRGHRKTYIGAMPGRIIQSIKKVNS
                     SNPVIILDEIDKVGQDFHGDPASALLEALDPEQNFAFHDNFLEVEFDLSKVLFITTAN
                     NIATISPALRDRMEMIEVSGYCMEEKVQIAKRHLVPNQITEHGLNSSQLQLNEKTIEE
                     IIKNYTRESGVRNLNQRIAKVVRNVAKKVAFEETYNPRLTIKEVRSILGVPRYNSDSS
                     IGNELAGVVTGLAWTETGGEILFVETSLSKGKGNLTLTGNLGDVMKESAVLALEYIKA
                     HANLLGINPDIFERWNVHVHVPEGAIPKDGPSAGITMVTSLASAFTQRKVKSSIAMTG
                     EITLRGKVLPVGGIKEKILAAKRANIMDIILPFENKKDVLEINSDYIKGVKFHYVGNI
                     MEALTFALLPEKVTNPICLEEPPRNN"
BASE COUNT         6684 a         5413 c         5245 g         5901 t
ORIGIN      
        1 ccttgctggc aggtttgaac ctatttttaa tcaccgaaag gttatcgaaa agtggttgca
       61 tgtatgggct gattttctcg ttgatgtcgc caggcaggaa accgatatct ttattcgaga
      121 gggctactat gggcctggcc agaagtattt ggttgaagtt ttcctcctgc tgaagagctg
      181 cagccagggc tagcagggtt tttcctgtgc cggcttttcc tgtaagggcc accagcttaa
      241 cttccgaacg taacaaggca tctagggcaa aagtttgctc ggagttacgg ggttcaatgc
      301 cgtaagcacg cgttttttca accctgttca gcatgttggt ggccttgtcg aaatgcgcta
      361 gtgcgctcga tttattaccc ttaagaataa ggtactggtt ggcgtagggt ttaaccttaa
      421 gcggtgcagc acctagttcg cttggatttt cgtaaagttt ttggataaac tcgtcgtcaa
      481 ccccgtcaat aatttcaatt tcctttttca gggagtcaat atcctgaacc ttatcggtta
      541 agtaatcctg agcaacaatt cccaacgatt ttgccttcat cctcaggtta atatccttgg
      601 taaccagaat aaccggcctg tctggattaa cagatgccaa atggtaggta atggccagta
      661 ttcggtggtc gggagtagcc tcggcaaagg agtcgagcat ggctttaggc atgggtttcc
      721 ctgtttcaat ccgtagcttg ccgagattct tccccaggga aactcctccg ttgaataggt
      781 tatcacctgt gattttatcc agctcacgaa caaactcacg ggcgttgtag ttaaccattt
      841 cattcccctt tttaaagtag tccagctcct ccagcgcaac tatgggtatt acaatgtcgt
      901 tctcctgaaa attataaatg cagtggtagt cgtggaggat aacattggtg tccagcacga
      961 atattttctt acttttcttt gccatgatct gttgatttaa tgaaccttta aatataacac
     1021 aaatagactg ttttagcttg aattgcttat cacctatcta aataattgag ctaaaaaaca
     1081 ctactctcct ttcccgattt attgtaaata attgtgtgcc agcgcatata atcatctccg
     1141 ggggaggggt gataatcttt ggccagccat taacttaaaa caccatataa aatatcgaat
     1201 tgaacacaat ttataaaaac aactatatgc cattaataat caggcatttt gtccaatttt
     1261 ctatttagaa ttaagttaaa tgttggcgtg agttgataat tatcaacttg atttttattt
     1321 agactaattc taactttgtc gtgaagttta accaaggaat agtaactaaa aacctacaaa
     1381 tatgtcacag caagagttca ataccgcact agtggagctg gaaccaaacc tggaacgatt
     1441 tgcctacagc ttaacagcta accgcgagga tgccaacgac ctgctacagg aaacctacct
     1501 gaaggcgctg acctacaagg ataagtttga ggacaacacc aacttaaagg cctggacctt
     1561 cacgataatg aaaaatacct tcatcaacaa ctaccgtaag gcggttaagc aaaacaccac
     1621 attcgacagc agcgaaaatc aattcctgct caacagcagc tacgagaaga atggacccga
     1681 gtccatgtac tcccacagcg aaattagcaa gaaaattgac cagcttgaag atgaatttcg
     1741 gattccattc cagatgcaca cttcgggcta caagtacaag gaaatagcag aaaagctcaa
     1801 ccttaaaatc ggcacggtga aaagcagaat tttcttttca cgccaaaagt tgatgagcgc
     1861 ccttagggac tacgagtaag gtgctaggca acaacacata ataggtagca ggagccatcg
     1921 gagattgatg gcttctgcaa tttttagtca aaaccttttt aaaacgaagg aaacatttag
     1981 aaaacactca aaggctgata cttatcgaat atcatgtggg taaaagttgg aattagcata
     2041 aaactccact atatttgctg cctgaaatgg ccccgtagtt cagctgaata gaacgtcaga
     2101 ttccggttct gaaagtcgtg ggtttgaatc ccgccggggt cacagaaaag gtcaacctgt
     2161 agtggttgac ctttacttta acaacatgct cccaacattc gctagagcgt ttctgctcaa
     2221 ccatgataaa ccaacatcgg gcgatagctt tcaagcccgc attgcccatc taaaaatgcc
     2281 agcagacttc taagaatcgg catattgtag aggttaaaca atgaatagca attattttga
     2341 ttaaaaaata atatatttgc ccgtcatttt atacatctaa aaatcataca acctacttaa
     2401 tacttcaaaa gatgcagaac aaaggtgcga taagatttat tgccatagcc ttaatattgg
     2461 tttgcatata ccagctgtca ttcacgttgg ttaccaggcg agtagaaaaa aaggcgcgcg
     2521 agtatgcgat ggtgggcaac gagatagaca gggctaagga aacccactac atggattcca
     2581 tttcgtccga gcctgtttac aacttcctgt acatccgcaa gtacacctat aaggagtgca
     2641 aggagcggga aataaatctg gggttggatc ttaagggtgg catgaacgtg atgctggagg
     2701 tatcggttac cgacctgata cgctccctat cgaacaacag ccaggatagc tcattcctga
     2761 aagccatgga ggtggcccag aaaatgcccg gcagcgagca ttttgtaaca aaatttggta
     2821 aagcttttca acaggttgca ccaaatgcaa ggctggctgc aatttttagc accccagaac
     2881 tgcgggataa gattaacttt gaatctacaa acgatcaggt tatcaagatt ttaagagctg
     2941 aggcagataa tgccataaac aactccttta acatcctccg taatagaatt gacaagtttg
     3001 gtgttgccca acccaacatc cagcggctgg aaaactcggg tcttattctg attgaacttc
     3061 ccggtataaa ggaacccgaa cgtgtacgaa agctactcca ggggacagcc aacctggaat
     3121 tctgggaaac ctacgagttt agcgaacttt tcaacaactt tattgcagcc aacaacgcgt
     3181 tggctgagat tcaggatgct acggctaaga cggattccat taaaagcacc agcaacaagg
     3241 agctaaccgc caaaaaggat accgtaacag atgagctgct ggccaagctg aaagccacct
     3301 caaccgagga ttccctttca gccactcagg ccgaccagac aaaaaaatac ccgttattca
     3361 gcctgcttca acccaacctg taccaggatc agcccgcccc cgggcctttg gtaggctacg
     3421 cccatgtaaa ggacacagcc agggttaacg aaatactaag gatgcctaag attaaggcac
     3481 ttttccccgt tgacgtgaaa tttttgtggg gtgtaaaacc tccaaaatgg gataaatccg
     3541 agtcgctcta cgagcttata gccctcaagg taaccagccg cgatggcaag gcacccctcg
     3601 atgggggtgt aataaccgac gccagagagg cgtttggaga gacaaagggt agcgccgaag
     3661 ttgacatgtc catgaatgcc gaaggggcaa gagtttgggc gcgcatgacc ggtgaaaata
     3721 ttggtcgctg tatagctatt gtacttgata actacgttta ctcctaccca agggtgcaga
     3781 gcgagattaa gggtggacgt tcacaaataa ccggtgattt taccatcaac gaggcaaaag
     3841 accttgcgaa cattttaaag tctggaaaaa tgccggctcc cgcccgcatt gttcaggaag
     3901 agctggtggg cccatcgctg ggaaccgagt ctattaattc aggtatgaac tcctttatcc
     3961 tctcctttat cctcgtgctg ctcttcatga tggtatggta cagcttcaag gcaggcttgg
     4021 ttgcggatat cgccttgttt ttcaaccttt tcttcatcat gggaatcatg gcctctttcg
     4081 gggcaacgct taccctttcg ggaatcgcag gtattgtgct aacgctgggt atggccgttg
     4141 atacaaacgt tctcatcttt gaacgaataa gggaggaatt acgatcggga aaagggacta
     4201 taccctcagt caacgagggg tataggaatg ccctctctgc cattattgac ggtaacgtta
     4261 ctaccctgct aaccggaatt attctctacg tatttggcac aggcccaatt cgaggttttg
     4321 ccaccacgct catcatcggt atcattacct cccttttcac cggaatactt atctccaggc
     4381 ttattcaaac ctggatgata aaccataaca tcaacatccg gtactccgtt aagtttaccg
     4441 agcatgcctt tagaaatcta cacattgatt tcatcggaat acgaaagtac gcctacatct
     4501 tttccggttc actttttttg ataatattta tatcgctggg tgtaaggggg ttgaatcagg
     4561 gtattgattt tacgggaggc cgctcgtatg tggtaaggtt tgacgatgcg gtttcgacaa
     4621 ccgatattgc ctcggaccta aagcatgtgt ttaacgaggc gcccgaggtt aaaacatttg
     4681 ggggtcagaa ccaggtgaag attaccacca agttcaaaat agactcaaag gatgaaaata
     4741 ttgagaacga aatggactcc ctgctctacg ttgggctaaa accctacctg caagatggaa
     4801 ccaccctgaa ggattttcaa accacctaca agcaaagctc cataaaggta ggacctacca
     4861 ttgcacagga catcaagcgc gatgcggtaa ttgcggttat cctggcacta atcgtgatat
     4921 tcctttacgt gcttattcgt ttccgctggt ggagctatag caccggtgct atagtatcgc
     4981 ttttccacga taccatgatg attgtgggaa tgtactcgct gctgtacttc agggtgccct
     5041 ttgggctcga ggccgaccag tcgtttattg ccgccctgct aaccattatt gggtactcca
     5101 tcaacgacac cgttgtaatc ttcgaccgta tcagggagta caacaagctc taccccaagc
     5161 gttcatcacg cgataacatc aacaacgcta tcaacgacac cctcagccga acctttgtaa
     5221 cctccttcac cgtgcttatt gttttagttc ccatgttctt ccttggtggc gaggttatca
     5281 ggggctttat ctttgcctta atcattggta ctataagcgg gtcatactct accgtattca
     5341 ttgcaacacc tattgcctac gaccttgaaa aagctgttga aaaacgtaaa aacaggaaaa
     5401 ttaaacagta aaatcccgta gcaataatta ggagcattgt taaaaactca acccacgcag
     5461 ggttgagttt ttttttagta gaggttaaag taaataaaat ccgtagcagc cacaaatcaa
     5521 cccaaagtaa ttgcttcaat atcaaaatac taccttatat caccattgct gcatcgctcc
     5581 ctgccattca acatggaaat tgtacggcat aaagttgtat ttttgtagtt aaacataacc
     5641 tacgcaccac aagccatgga aattgtactt agcggaatac gctcaacagg caagcttcat
     5701 ttgggaaact actatggagc tttgctaaac tttgttcaaa tgcaacacga gcacaagtgc
     5761 ttctttttta ttgccgacta ccactcgctt accactcacc caacacccac agatttgcat
     5821 ggatcggtta agcaggtttt agccgagtac ctggcatgtg ggcttgaccc cgaaattgcc
     5881 acgctatacg tgcaaagcga tttgccccag gttccggagc tctacctttt actcaacatg
     5941 aacgcttacg tgggagagtt ggagcgatgc acttcgttca aggaaaaaat acgtaaacac
     6001 cccgacaaca taaatgccgg gctgcttacc tatcccacgc ttatggccgc cgacattctt
     6061 atccacaagg ctaccatggt tccggtggga aaggaccagg agcaacacct ggagatgacc
     6121 cgcacatttg ccaaccgatt taacaggctc tacaaggtgg attactttcc cgaaccagtg
     6181 gcatacaact ttggaaagga cctcgtaaaa ataccggggc ttgatggttc gggtaaaatg
     6241 gggaaatcgg aaggtaatgc catatacctg tccgacgacc caaagaccat acgtagcaag
     6301 gtgatgaaag cagttaccga ttcgggaccc acccaaccaa accaggaaaa atcggagccg
     6361 gtaaaaaacc tttttaccct catgcgggta gtttccgatc ctgaaacgta cagctacttt
     6421 gaggagcaat acagctcatg ccatattcgc tacggggata tgaaaaaaca gctggcagag
     6481 gatatcatac ggattaccac gccaataagg gaaagaattg aggaaattct ggcaaacgat
     6541 gcctacctaa gccaggtagc acaccatgga gcagaaaaag cgcgtgagag cgcatccaaa
     6601 accattaacg aggtaaggca gattattggt ttccgaaatt tttaggaaac gatttgcgag
     6661 agcaacatga acagctaggt ggtaaaatca atccacccaa caaaaaaaca tcctaaaacc
     6721 tggcaagcca accatagcag acaagcagaa aacatcctac tattttcaag cggaaaagcc
     6781 ccattcaaaa ataattgtac cagcaggatg ttatattaac acgattaatt ttataaattt
     6841 gttcgttcac ccaaaactac cctacaagtt ggaattagag caagtaattc agctgcttct
     6901 gtcacagttc gattttgtca gcctacctgg attaggcagt tttgttaaac gtcgtaatcc
     6961 ggcaaaagta tcggacgata ggtcaagcat tgaaccccca agcgagtact acatttttga
     7021 cacatcgcgc acgtttgacg acgaggctat tacaaactac ctgtgcagca cactgggaat
     7081 tgatcataag gaagcagaat taagggtaac gcaacttgtg gagagggtaa acaatgcact
     7141 tagcagtaga aaacctttcg agttccccgg tgttggaact ttgttaaagg atgacaacaa
     7201 caatatcact cttgaatttg atgccagcgc agctgcaacc gagtcgtatg gactttcagc
     7261 aatagaagtg ctccctaagc aaaaagcaga ctccaaacta accagtccga aggaaaacaa
     7321 aaatgcagct gtagccgaaa cccaggcgtc cagtccacta tcaacagagc aaaccaccaa
     7381 aattccgact ccaaagccag ctgttgcaaa aaaatcgcgg gttaggttat gggtccccat
     7441 tacagcctgc atagtggtgg tagcagtagc agtgtgttgg tttactccag cattgcattt
     7501 ttgggagcaa tcgcaaacct cggtttctcc atcaacaacc gatagctcgt ccagcatggc
     7561 atccgtaggt aaccttcctt tagcttctgc cattccggat tccatcgtag aagaagagga
     7621 ctcgcaagtg cccgatagca tatcggcctc aggccaacct aatgccaccg atgagggtgg
     7681 agccgaaaag attctggaac aaaccaccag taagaaagta gcgcttagct acacagagcc
     7741 accggtggtt gaaacgggta aatctttcta cataattgcc ggcagcttta caagcgaggt
     7801 taatgcccag aagttcctta gtgagctggc agaaaagggc tacaaaccaa cccttatacc
     7861 acgtaacaag ctctacagcg tttcgctcta tacttttagc aaccgcgaca gggctttgcg
     7921 ggagctggaa agattaaaat cagagcaaac atttgaaaaa ttatggcttt taagtcagta
     7981 gagggtgaac cggttgtaag aaaataggcg ctactactaa acaacaaaaa gtagccagca
     8041 cctattccta aagaatagta tttctacttt acagttgagc cattagaaac cttggctgcc
     8101 ggcgaaacca gctgtagctt tccacttgta tcctccgcca tcagaatcat gccgtgcgaa
     8161 tctattcccc taatttttcg cggttcaaga tttgctaaaa ctagcacttg tcgtccaacc
     8221 atctcctcgg gggtgaagta ctcggcaatt ccggaaacca aggtgcgtac gtcaattccc
     8281 gtatcaactt taagtttaag cagcttttga gttttaggaa ctctttcagc atcgagaacg
     8341 gttgccaccc gaatatccat tttagaaaaa tcgtcgtagc ttatagaacc cttttgttcc
     8401 ggaacatttt taacagaatt ctggtttacc agcttggtgg cttgaagctt atttagctgg
     8461 acagcaataa catcatcctc aattttatcg aatagcagct ggggtttgtc aagaatatgc
     8521 cccggggcca gcaggctatc taacccggca agcttccatt ccttactctg aaaattaatc
     8581 atcttgcgta tcttctctgc agtaaatggc agaaaaggtt caagtataat ggagaggttc
     8641 gctgtaatct gcatggcaat gttaagaatg ttggcaacct gccggggatc cttctccata
     8701 agcttccatg gttcagtatc agctagatac ttgttgccca aacgggcaat attcatggct
     8761 tctttaagcg cttcacgaaa tttaaactct tcaaggttgt gctccaacct ttcctttata
     8821 cggggcaatt cgtgcaaaac tgaattatcg taatccgtaa gagcgtttct ggcaggcagt
     8881 ttgccatcga agtacttatg ggtaagaacc atagccctgt taacaaaatt tcccaggatg
     8941 gcaaccagct cgctgttgtt acgggtttga aaatctttcc atgtgaaatc gttatccttg
     9001 gtctcgggca tgttggcggt aagtacatac ctcagcacat cctgcttgcc gggaaactca
     9061 accaggtact cgttcagcca aacagcccag ttgcgagagg tggaaatttt atcgccttcc
     9121 aggttaagaa actcattggc cgggacatta tcgggtagaa tgtaatctcc gtatgcttta
     9181 agcatggagg ggaaaataat gcagtgaaaa acaatgttat ccttaccgat aaaatggatt
     9241 aaacgggtat cgctatcctt ccagtatttc tcccagtttg gggtcaactc cttggttgcc
     9301 gatatatagc caataggagc atcgaaccaa acgtaaagca cctttccctc ggcaccatcc
     9361 agcggaaccg gaacacccca atcgaggtcg cgacttaccg cccggggttg taaaccctgg
     9421 tcgagccaac ttttacactg tccgtaaaca ttggctttcc actccttgtg gtcttcaagt
     9481 atccactttt caagctcggg ttgaaatttg tctagcggca aaaaccagtg cctggttgct
     9541 ttcagtatag gcttggaacc gcttagggtt gaccttgggt caatcagatc ggtagcatta
     9601 aggctagttc cgcacttttc gcactgatca ccataagcgt tgggattccc acaatgcggg
     9661 caagttccgg taatatacct gtcggccaga aattgaccca cctcctcatc gtagtactga
     9721 tcggtaacct tttcaacaaa aacacccttt tcgtagagct tcttaaaaaa ttcgctggca
     9781 gtttcgtagt gaacaggcga agtggtgcgg gagtatatat cgaaatcaat tccgaactgt
     9841 ttgaatgatt cttttattat ggcatggtac ttatccacaa cagcctgtgg agttgtattc
     9901 tcatgcctgg ccttgatggt tatgggaacc ccatgctcat cggagccgca aacagatata
     9961 acatctgcgc cttttgctct aagatagcgg gtgtaaatat cggaagggat gtaaacaccc
    10021 gccagatgtc caatatgcac aggcccattg gcatagggca acgcagaagt aataagatat
    10081 cttttatagt tactcattgg gtgaaaaggt ttatacaaat tgttgcaaat ctacatattt
    10141 atggccaaac gtagagcggg aaaagtaaag ttgttaccgc taggcggcaa gaagaaagaa
    10201 gaataaggga attaaaattg atacagccgg agccggacaa gtttcgccgg taggcaaaca
    10261 agtttttgaa tcctttatcc ttgcttatac catcctgcat aaaacaggta gttacgggca
    10321 attcgctcgt tcatctcacg gctaacctcc gcatcaacct ttttgataaa cttggctgga
    10381 acacctccgt agattgtccc cggttcaaca gtagtatttt ccagaacaag cgcattagca
    10441 gcaacaattg caccctcgcc aataaccgca ttatccataa ccgtgcatcc cattcctagc
    10501 agcgcaccat cgcgaacttc ggcaccatga acaattgcac catggccaac cgatacatcg
    10561 ttgcccagcc tgattgttgt tttttggtag gtggtatgca aaaccgcgtt atcctgaatg
    10621 ttaacccggt tgccaatccg gatggagttc acatcccctc taaccacaac gccataccaa
    10681 atgctacagt catcgccaat ttctacatct cctatgatta ctgccgtttc ggcaataaaa
    10741 cagttttccc caatttttgg agtaaatccc ctaagtggtt tgattattgc catactttag
    10801 tgtatttttt cacaaaaata gcaatgaaaa ctattgttgt aaaaattcac ctgtttaaca
    10861 ggatggaatg gggaaattgg tttacaaatc cattaaaatc tgccaggttg caccgaataa
    10921 atttaaaatg gtaaaaaaat tctcaaagca tatcgagctg cgcttctatg gcggtttaaa
    10981 ccatttttta ccggcgtcga aacggcaggt tccatttaac ctaccctaca tgggaacacc
    11041 cacagttaag gaccttatcg aatcggttgg agtaccgcat gtagaggtat ttttaattgt
    11101 ggctgacagc atgtcggttg gttttgacta caagcctgaa cccagctccc aaatttcatg
    11161 ctacccgcga ttttacaacc ttagcataga ttacggaata ctacgaccaa ccgagcccgc
    11221 cccaccggta ttcatcctcg acgttcacct ggggcgtctg gcatcctacc ttagactata
    11281 tggcttcgac acgctctata gcaactgcta ctccgacgac caaattctca ggcttgcagc
    11341 cgcagagaat agaataattc ttacacgcga caagggaatt ctccggagcg gagcagccat
    11401 ccatggctac tatgttcgtt cgactcaacc caaggagcag ctcaacgagg tagttgatta
    11461 cttcagcatg gcttgctttg ctgacccctt ctctcgctgc tcgctatgta acggtttagt
    11521 agaaactgtt gatagggaaa atgttaaagg tcttgttgcc gaaaactctt ataaccatta
    11581 ccactcattt taccgttgca gccggtgcgg acaaatatac tgggagggat cgcaatataa
    11641 gggaatactg aatttatttg gcaggcatgg cccacatcat gaatgattaa aaaaaggtga
    11701 aaaaggcatt cacccaaaaa gatatgttat taaatattat ttaattttat tgcctaataa
    11761 taaaaagggt aattttgcaa agcataactt ataattccta agaaagtgag catttttagt
    11821 aatttattgt tatacaaatt ataaaattag gtactagtta aaggcgtaat aaacttatta
    11881 ctatggagaa acaaacaggt gttcaagagt tggatgaggt tgctgtccga ttttccggtg
    11941 attccggtga tggtatgcag ctaacgggaa cgctttttgc cgatgcctct gcactttttg
    12001 gcaatgatgt aagcacattt cctgattatc ctgccgagat acgagcccca caagggacta
    12061 ttggcggcgt ttctggtttc caggttcata ttgggagtaa aaaggttaac accccgggag
    12121 attattgcga tgtactggtt gctatgaacc ctgccgcgct gaaggccaac attaaatggg
    12181 tcaaaaaagg aggcatggtg attgccgatg ccgatcagtt taatgaaaaa ggctaccagc
    12241 gtgccggtta taaaaccctg gatgctgtaa ccgagctcaa cctacaggac tacaaggtaa
    12301 tcatggcacc aattaccact cttacaaagg aaagccttaa ggatagcggc ctggataata
    12361 agagcattgt taggagcaag aacatgtttg cgctggggat ggtttactat attttcgacc
    12421 gtccgatgaa cttcaccgaa acatactttg aaacgaagtt taagaagaag cccgaaatta
    12481 ttgaggcaaa caaaaaagta cttcaggacg gctacaacta cgcagcaaat attcaggcca
    12541 ttccaaatac ttacagtatt aaaccatcaa aaatactgcc taaaggaaaa tacagaacgg
    12601 taagcggtaa caccgctacg gcatgggggc taatagctgc tgccgaaaaa gccggactgc
    12661 ctctcttctg cggttcatac ccaataactc cggcaaccga tattttggct gaactttcca
    12721 agagaaaaga tttgggggta aaaaccctgc aagccgaaga cgagattgcc ggcatttgca
    12781 ccgccattgg tgcttccttt gcaggcaact ttgccgttac ctcgacttca ggccccgggc
    12841 tatcacttaa gtcggaagct ttagggttag cagtaatgac cgagttgccc ctggtggttg
    12901 tggatgttca acgaggtgga ccttcaacag gtttaccaac caaaaccgag caggccgacc
    12961 ttatgcaggc gctatgggga agaaatggcg aggcaccagt agtggtaatt tcggcaacct
    13021 ccccaagcaa ctgttttgat gcggcgttta atgctggtaa aattgccatg gagcacatga
    13081 ctcctgttat cctgctaacc gacggcttta ttgccaatgg ttcggaacca tggcttatcc
    13141 ccaacatgaa ggagtaccca aaaattaacc ctcccttggt agaggaaggc acccagggtt
    13201 acatgcccta cgcacgcgat gataagcgcc ttgccagagg ctgggcgctg cccgggaaaa
    13261 aaggtcttac ccacagggtt gggggcctcg aaaaagactt tctgaaaggt tcgctttcgc
    13321 acgacccaat aaaccatcag cgaatggtgg atattagggc tgccaaggta gagagggtca
    13381 gggagtttat ccccgaacaa aaggtgattg gcgatagcga aggcgacctg ctggttatcg
    13441 gttggggtgg cacctatggc cacctgctcg atgccgtaac cgacctgcgc ggaaagggga
    13501 aaagcatttc gctttgccac ttcagctata taaacccact gccaaaaaat acccgggata
    13561 tactgagccg ctataagaag ctggtggttt gcgagctaaa catggggcag ttcgccaact
    13621 accttcgtat gaattttcag gagttcaact acctgcagtt caacaaggtt caagggttac
    13681 cctttaccgt ggttgagctg gtagaaaagt ttaaccaatt actggaggga aaataaccat
    13741 ggaagaacag tacttaaatt atacacctca ggatttcaaa agtgaccagg aagtgcgctg
    13801 gtgccccgga tgtggcgatc acgccattct gagtgcagtc caaaaagcgc tacccgaggt
    13861 ttcaaaagag ctgaagtaca acatagagcg ctacgtattt gtttcgggaa taggctgctc
    13921 aagccgcttt ccttactacg taaataccta tggctttcat ggtattcatg gtcgtgcctc
    13981 ggccattgct accggtgtta aagttgccaa tcccaccctt agcgtttggc agattaccgg
    14041 cgacggggat gccctggcta ttggtggcaa ccacttcatt cacgccattc gacgcaacat
    14101 tgacgtgaat attctactat tcaacaacca aatctacggt ttaaccaaag gtcagtactc
    14161 ccccacctcg ccacttggca aaatcaccaa aacatcgcct tttggcactg ttgagcaccc
    14221 attccggcct ggtgagctgg ttattggcgc acagggaaag ttttttgccc gtacccttga
    14281 tgtggatgtt aagctcacct ccgaaattat ggttgaggcc gctaagcacg acggcacatc
    14341 ggtagtagaa attctacaga attgcgtaat ttttaacgat ggggcttacg acttcatcag
    14401 cagcaaggaa accagggacg acagggttat tgtccttcat cacggggaaa agatggtttt
    14461 tggcaagaat cgggataagg gactagtgct cagcgggttg agcctaaagg tggtaaccat
    14521 tggacaggga ggtttcacca tggatgatat cctggttcat gatgcctata accccaaccc
    14581 cggcgttcac atgatgctgg ccaacatgga gtttcctgaa tatccgattg ctcttggcgt
    14641 tatcaggaat gtaaaggatt caacctacga cgataatgtt cgtgatcagg tgctggaggt
    14701 aatgaaagat gcaaaaatca aatgcgttga cgacctgctc aacagcggcg aaacatggga
    14761 ggttaagtag gatacctgcg ctgcatttaa tattgattta attttttact tgtacaagta
    14821 cgaccggggg gttccccggt cgttttcatt ttatttcaac acctaagatt caaccccaat
    14881 gggtgtgaaa tacatctact actgcttgat tacctttttc accagctttt cgccgctggc
    14941 tgactccatg gtaataaggt aaaatccggc cggcaggtta gctgtgttga gtgtataggt
    15001 ctccgatccg tttcctctaa actccatcac cacctgcccg gtaaggcttg taagcttgat
    15061 atatcccacc tctgctgcgc cgcctatccg taagtagctg tcaaaggggt tggggtaagc
    15121 tgctgctgca tttagtttgg aatcgcctgc cgacaggggg aaaagtaaaa tatctatgtt
    15181 tttatcccca cctgagatag taaatgtatt ccccagaatt ggattatagc cggttttggt
    15241 aacattgtag ctataatctc catctttaag gctggtaagt gcaaccccga gggagttcgt
    15301 atagataacc gggtatccct caatggcaat ttcagccccc tcaacagctt caccgttggc
    15361 atatacactg aaagttacag gatagctggc gctcgacaac tttatggtat ccagcatcat
    15421 gccaccatca acggtaagag tgtccgagta gcttacaaaa cccttaccag acacatttag
    15481 aacgtagcta tcaacaagca ggctgtcaat agagaatctt ccgtcctcat tagttgtggc
    15541 gttgtaggtt agaaatccct gcatggagat attggcaccc gggattgcaa ccggtaagtc
    15601 gttgctgaaa attaaacccg aaacgctatg attgggaaaa tcgctaagcc caaactgcag
    15661 gtaaatattt ggccgcaggc tcgaattatc atcgtagccc ggcattggat tgtcgtcatc
    15721 gtacccataa accaccgagg taaaaggtgt agagctgcag gccacctcat aggcatcatt
    15781 ctcccacgta gagttcaacc caaagctgat ttccaccaac aggttgccgg tagcggtgta
    15841 cctaaagtcc tcaatgtcga atacatgcca tccagccgtg ccgggcatcg tgtagctagc
    15901 agcgttgagc accaaagtag ccgaatcggt atttacgtag ctagctccca aataggatag
    15961 ctctgtctct aaaagtttaa ttttaaggtt ggaaaacggg ctgatctccc ccacctgcat
    16021 gaagtaaaat cctatagact taattgtttt agggtttgcg gcaatttctt cctttttgaa
    16081 aagaatctgg ctgcggtttg ccttgtagta gctatcgaat ggataggttc cttctgccgc
    16141 caagttgcca gtgcctatcc tcacctcggg cttatcgcct atgccgaagt gataatttcc
    16201 ttcagcagtg taacccccct tagaaaccgc agctgttacg ttaacctcgg ttccaacaaa
    16261 cgtacctgaa ctggctgata cgtcgaaatc aaccggtaaa tcggaattga gaggtaacga
    16321 aacgattttt gaaggattgt tgatatttaa gaaaggagaa tcatccgaga ggattaccag
    16381 ggcatcacca gcatcggcgt gaccctggtt cgatagtaat accctaagtt gcccattttc
    16441 tcccgggtcg agctggccgt tggagttgtc atcgcccact agggtaaaat cttttaccga
    16501 cagcttgggt gaattcaccg taatccacag cttagatggc cacgtgctgg tcccgtcggt
    16561 agcatcaagg ttaaaagttg ccacatacct atcgggaatg ctgtcggcca cggctagctc
    16621 aaacgctgcg tttaccgtcg aagtatttcc ggtctcaccc gaagggatat cgccaaagta
    16681 aaccgagtcg ccatccgtta tcgtaacata cctatctaca cctgaaagcc ttgccgatac
    16741 ggtattggcg atgtcaacac caacattttt taaggttacg gcaatcgtaa acttttcacc
    16801 gaaatcagcc ttggcattat cgtcctcaat ggtgtataag tcaagcgtga tgtagggccc
    16861 gtccaaagcg gcagcgggta tcctcgccat gtagggaatg gtttgtggtc gggtaaccac
    16921 aatatccaca tcgccaccct ccagaacggg attaaccgga acatcaacct cacctgaagc
    16981 gtcaaccagg gcagcgccat gtagcaaacc atcctttgaa atggcaacgt aagaacccgg
    17041 aagtgcgctg acagtgtaaa aagacagccc aataggaaag ctatcggtat gcgaaactgc
    17101 atttgggcta gcctcggtaa agtaaggcac aagtgaagga tcgcccagaa tgttataggc
    17161 ttcccagtag taatgcggac tcgattgctg tgggtatccc tgggcatcaa cctcggttac
    17221 ggccaggtta ccggcaaaca ccattccacc ggtagtaacg taattgccat gatggggcgc
    17281 atcgtaggcg ccaagggtag tttcctcgaa ggtaggaaca tacccatcat tttcgcccac
    17341 catgggaaat gcaccaacag accagtagaa atcttcaaac caatatgttg agggagaaga
    17401 accaatgtag gtaacagctc ctttaccggc agctctaatc catttctcgc caatacactc
    17461 atcggttgca aagtcgcctg ataggcagca gtttccaaca gccagggggt acctgtttag
    17521 attggtcatt gcttcaacag ttgaagatgt aagagttgga tcggcccatg aggtctcgct
    17581 gcaatgggcg gtgtagttaa taaacccaac agcaacctga tcggaaccat agcaaccgtt
    17641 gtaacccggt tgggcacctg gattacttgg atcgttagaa acgccatact cgtaaatgct
    17701 tgagtatccc tgagcattat tgaagtagta cttggttccg tactttatgg ttggctgacc
    17761 aaccttcgga ttccaattgg catcggcacc ggcaatcagg gtggccgaat taagataggt
    17821 tggatcctca aacccgtact gctcgtagta aagtatctta tctataatat tctgaagctg
    17881 aacatttgta ctagccgaca tgcgcccgta gtacatctcg ggaaagtagt cgccatcaac
    17941 gcttgcatag taaaggtcgg tttgcttgta agaggcggaa cccacgcccg aggcgggaac
    18001 ctgggccacg tcgcccacaa ggataagaaa tgttggtgca gggttttcgg gagtagcgtt
    18061 tgtgtaggaa ctatggacga aggttttgat tgcttcggcc gtgttaccaa tgacatcggt
    18121 ataggccacc tcaacgttga accctttcct cgttttccac tcaacaaagg ggacaagggt
    18181 tgattcaaac atcctgttcg aaacaattag catcttaacc ggataggtgg tcaagtcggg
    18241 atgatcgtca aaggaacttt tggtaaggtc gttgattaga aagctgtagg atgtctcaaa
    18301 gtatggtgaa gaagttttgg caagcatttc atcaaactta gcctggctaa ccccctcaaa
    18361 gtcaacctca acatcaatat cgttgtatat tctaagcata ccggttaccg gattataggc
    18421 cacagggttc acttcaagcc ttgccacctg aactccacgc atggttccca gcatatcaac
    18481 cttaaccagc tgaggttcta tcatctcgtt cttacggtaa acctcagcct tcatgatgaa
    18541 ttttaatgag tcaagattct gatccttacg aacaggaggc tgaacgggaa taacaggatg
    18601 gttaattccg taatcaccta gcgcaatatc aacatggctg aaactgtgaa cctttactat
    18661 tggcttggcc cctagtggaa cttgaatgag cttcttgtag gcaggcagtt tgggagtgcc
    18721 aacaagtccg gtcgaaaacc catctggaat agccaggtta acatactttt cgcccgattc
    18781 cgttaccact tcatttgata taagcgagct gaaactaacc gaaaccctta gctgctgaag
    18841 agtgctctgc ttaacgctta tggccgattt actacccttt aagggaataa ctttttgctg
    18901 ggcaaaaaca gcagaactca agagaattac agcggtaaaa atggatagta tccttttcat
    18961 cacaggaaaa atattgaatc aaccataaca acaaacattg tgcaagttaa gctattatgt
    19021 taaaaaatgc caatttagag ctttcatgtc ctgcctaaaa aagttttaca aattagcaac
    19081 aaggggaagc aagcagttca gccaagaccg gcagcttggt aaaattcata gtagccagaa
    19141 aacctctaac aagcgaccga ttttttaact acgtttgtct taaatttaag gttatggcag
    19201 ctggttttat cctatttgat gatgatagaa gggaagccct tctccccttc acgtttaccc
    19261 ggccggtttc agaaattcga attggcattc taacaattac ccaaaaatgg gagcgttacc
    19321 ttggtagttc aacatcattt cttaccacag gctacctttc gaaaaagtat ccacaaaaca
    19381 ccactggtga aagattttgc ttgataaacg ggggtgtttg cccggataaa gcgctggtta
    19441 atgccataat gtcattagaa gcaggcgaag tgctagttca tgaaaatgtt gtgctagcag
    19501 gtgtaatgga taaactacct gccgaaacac catccctgtc ggattttcgt gtcagaattc
    19561 cctaccccaa cgaagtgctt caggtgaaac atctctggga tattttccgg ctaaacggtg
    19621 aagccttgac cagagacttc gacatgctaa cattaaacag aaaatcggca ccaatttcgt
    19681 caagtaaccg gactcttaac ccatccagga tatttattga ggagggtgca agggttgaat
    19741 gctcaactct aaatgccgaa gccggcccca tatatatcgc ctcggatgcc gaggttatgg
    19801 agggagccct tatcaggggt ccattttcgc ttggcgagca ttccgccgtg aagatgggtg
    19861 ctaaaattta cggccccacc actattggac cgcactgtaa ggttggaggt gaaataaaca
    19921 acagcgtaat atttggttac tcaaataagg cccacgatgg ctttctgggg aactcggtga
    19981 ttggcgaatg gtgtaacatt ggtgccgaca ccaacaactc caacctgaaa aacaactacg
    20041 cagaggttaa actctggagt tatgcggata acgcatttac cagcaccgga cttcaattct
    20101 gcgggttgtt catgggcgac cattccaaat gtggcatcaa caccatgttt aacaccggaa
    20161 cggtggtggg tgtctgcgcc aatatttatg gcagcggatt tccacgaaat tttattccct
    20221 gcttctcatg gggtggggcg tgtggattta aaacgcatca acctgaaaaa gctttggaaa
    20281 ctgcccgttt aatgatgcag cgtcgaaatt tggagctaac ccaaactgac atcgacattc
    20341 taaagaaaat ttttgacctg acaagccagt accgaaactt ttaagtgcaa ccatgtaaaa
    20401 ctttttggca taggtattgc ccatatcaat actcgaaaaa ataaatcgag ataatgacat
    20461 gttgtcatta ggcattgagg tatcagtatg gaaagaaata gcagaaattt ctatagcaag
    20521 cttcttattg atgactccaa cgagtcaaat gtgaatttta tacccattct cgccgacgat
    20581 ggtacacccg aactcaagga tgctgatatt ccggatgttt tgccgattat ggcgcttcgc
    20641 gatacggttc tctttccggg tattgttatg cctataacgg ttggcaggga aaaatcgcta
    20701 aaactaataa aaagcctacc cccgcgtaaa aggatgattg gagtggttgc ccaggttgac
    20761 tccaaaattc ccgacccaac tgcagaagat ttgtggcata ttggaaccgt tgcccaagtt
    20821 attaaagtgc tcgaaatgcc cgataggaat ttttcggtaa tactaaaggg aatttgccga
    20881 tttgaagtta agaactacct cagcgaagaa ccctttttta tggccagggt aagcacgctt
    20941 gcagacattc tcccagatga aagcgacaag gattttgctg caattgccag ctctattcgc
    21001 gatataacca tccggatcat taacctcacc cccaccctac cccaagaggc agcctttgcc
    21061 attaaaaacc tggacgacaa tcagctactt gtcaactttg taagcgcaaa cagcgacatc
    21121 agccctgaag aaaagcaaaa gctacttgaa aaatccatgc taaaggagcg agccactcag
    21181 ctcctccagt accttacacg cgaggttcaa atgctggagt taaaacagga catacagtcg
    21241 aaagtaaagc tcgatcttga gcagcagcaa cgggagtact tcctacagca gcagattaaa
    21301 accctgcagg aggaacttgg ggccaacccc acagaacagg aagtaaacga tctgaaagaa
    21361 agggcaaagc agaaaaaatg gagcaaggag atagccgagc atttcgaccg ggaactcagc
    21421 aagctaaacc ggatgaaccc catggctgct gagtactccg ttcagctcaa ctacgtggag
    21481 ctgctcctcg atctgccatg gaacgagttt actcaggata actttgacct tgcgcacgcc
    21541 agagaaattc tggatgagga ccattttgga ctcgaacagg ttaaggagcg cattcttgaa
    21601 cacctggccg ttttgaagct gaagggcgac cttaaagccc caatactctg cctgtatggc
    21661 cctccgggag ttggaaaaac atctcttgga aaatcggttg cacgagcact aggcagaaag
    21721 tacgtgcgca tttcacttgg gggtatgcac gacgaggctg aaattagggg gcatcgcaaa
    21781 acctacatcg gagccatgcc gggcaggata atacagagca ttaaaaaggt taactcatcc
    21841 aatccggtta ttattcttga tgagattgac aaggtgggac aggattttca tggcgatccc
    21901 gcatctgccc ttttggaagc gctcgatccg gagcagaact tcgcatttca cgacaacttc
    21961 cttgaggtcg aattcgacct atccaaggtg ctgttcatca caactgccaa taacatagcc
    22021 accataagtc ctgccctgcg tgaccgaatg gaaatgattg aggttagcgg ctactgcatg
    22081 gaggaaaagg ttcaaattgc caaacgccac ctggttccca accagataac cgagcatggt
    22141 ctcaactcct cccagctgca gctaaacgaa aaaacaattg aggaaataat aaaaaactac
    22201 acccgagaaa gtggcgtacg caaccttaac caaagaattg ccaaggttgt gcgaaacgtt
    22261 gccaaaaagg tggcgtttga agaaacatac aatccccgct taaccataaa ggaagtaagg
    22321 tctattctgg gtgttccgcg gtacaacagc gactcatcca ttggaaacga gttggccgga
    22381 gtagtaaccg gacttgcctg gaccgaaacc ggtggcgaaa tcctttttgt agaaacaagc
    22441 cttagcaagg ggaaaggaaa tcttaccctt accggaaatc tgggtgatgt gatgaaggag
    22501 tcggccgttc tggcactaga gtacataaag gctcatgcca acctgctggg aataaaccct
    22561 gatatttttg aaaggtggaa tgtgcatgtt cacgtacccg aaggtgccat tcccaaggat
    22621 ggaccatctg ctggaataac catggttacc tccctggcat cagccttcac ccagcgtaag
    22681 gttaaaagca gcatagccat gaccggtgag attactcttc gtggcaaggt gctgccagtt
    22741 ggaggcataa aggaaaaaat cctggctgcc aaaagggcta atatcatgga tataatcctc
    22801 cccttcgaaa ataaaaagga cgttctggag ataaacagcg actacattaa aggggttaag
    22861 ttccactacg taggcaacat aatggaagca ttgacatttg cactgcttcc cgaaaaagtt
    22921 acaaacccta tttgtctgga ggagccccct cgtaataatt aagcataaat aaacctcttc
    22981 ccaagcaaca atttaaagcc cttagaagac ggaataattt tggcgaatcc accaactctg
    23041 catcgggttt caccaacagt cggacaagtc taccttatcc tttaaccttt tctccagccc
    23101 taacgggaga aaaggtattc tccgcgttgc tcataaagtc gggaaacgtt gaacgttgcc
    23161 ggtataagta aaaacggcat aaaagcccga cccgcaaatg ggtcgggggc ggaacgtagc
    23221 gtgggaatac ccaagcggca ctg
//
LOCUS       DAOPHX010000024        23081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_2319_length_23081_cov_7.761791, whole genome shotgun sequence.
ACCESSION   DAOPHX010000024 DAOPHX010000000
VERSION     DAOPHX010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 23081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23081
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2319_length_23081_cov_7.761791"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..588
                     /locus_tag="PK502_03135"
     CDS             25..588
                     /locus_tag="PK502_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99842.1"
                     /translation="MLTIPNLFLVGGNSRHSGKTTLACEIIRRLAKEQEVTAIKFIRF
                     DPDEKEMHGSHLGESEFSGYSIMRETNNHSSKDTSQMLQAGAKEAFYIRSGEEFMEQA
                     SIDFLTRYNHGQPLVCESSALRHFVEPAVFVMMMRIPPIGNPKPVESYLPLADRILYF
                     DKSYDERLRMLEELRFDSGKFYLPSFK"
     gene            complement(567..2285)
                     /locus_tag="PK502_03140"
     CDS             complement(567..2285)
                     /locus_tag="PK502_03140"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HPG99843.1"
                     /translation="MKQSQTTLNEKWIKASILGSMWASVEIVWGSFLHNLRVPFSGHI
                     LTAIGLIILISVSYRWREKGLFWRAGVICALLKTMSPSAIILGPMIAILSEAILLEAA
                     VSLLGKNRIGFTVGAILAMSWNLVQFIISKVIAYGYNLVKLYESIMQYVQKQLNTNID
                     MVWAPIILLLTIYILLGIGTAIAGIFIGKKLGSTDLNAEIFKAKNKASLLPTSKYTRF
                     PFSTAWLIADVLSIILVLVLAKRINVILWFGISILVVVCWIFHYKVALRRLGQPKLWI
                     SFFIITVIAAIAFSQFQSGISLWQGTLIGLEMNVRAIVMVIGFAVIGTELYSPKIKNL
                     FQKKALISLYMALDLSAESLPNMIALVPNLKEIFKHPLEIVKQLLSYAEYRMHEIESR
                     KQHKAAIAIITGEKSSGKTKFLTETATTLRENGIDVGGVYTKKIRENDEITAYLIVNV
                     ATGEEYPFLQKNELESELRIGAFSIDAEGLEFGKKNIVKSFDSRVLMIDEIGKLEMSG
                     GGWHNELNLLPLEKQLYILLSIRQEFVEDVIEKWSLHPAAIYNVAECNPEILIQEITA
                     YLKEGR"
     gene            complement(2368..>3154)
                     /locus_tag="PK502_03145"
     CDS             complement(2368..>3154)
                     /locus_tag="PK502_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPG99844.1"
                     /translation="NTSITNNQQNYSIQMNRNEHKLHKVENAKIMNSDFTCSTGVDDK
                     CRLNDKIDFTARLPYNYLKSLEADDQVTAADTTNFPKNNHHAANAQIAMSWMPNHKLS
                     VSAFVAQKTRFTTLRERHSYKMEQELFNLNFQSVKAAHSYISFQYNLLRNLPIDGSIH
                     HIRLNDVIKSECGVQGNLIQLQNAGKSRFNGADLSLHFALLYNLDLTASYSFIIQKKN
                     SNPSCGIVTNEFHLFNLQGSAWRKQFTLFAGIKNLFDANYAYA"
     assembly_gap    3155..3254
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3510..4028)
                     /locus_tag="PK502_03150"
     CDS             complement(3510..4028)
                     /locus_tag="PK502_03150"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPG99845.1"
                     /translation="MREKVEQTIHLQMNTTNISEALCTVSSVIFANMGGRFEPTVVVS
                     GFDIHGIRVFVDGLSLQKFYNGDTDMGQLATFDYSLVSLTKGSTPMSSGMNATDGSIN
                     LISFQPTDKLNLQVIAGLASGNTYEYGVNVGSRWKMFFFQSSYYQQRFSERETSKSDI
                     LLLPINNLRTII"
     gene            complement(4211..4552)
                     /locus_tag="PK502_03155"
     CDS             complement(4211..4552)
                     /locus_tag="PK502_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPG99846.1"
                     /translation="MNSNFQIEFRFWVSFDGKPLLGKGKMDLLRKIDQRGSLRQAAEE
                     LRISYRKAYYRIYSMNKAAKQPVVELKRGGKNGGVSEITTYGKKLMKEYEIIEKQLTD
                     FIQNCKFEAVH"
     gene            complement(4641..5345)
                     /locus_tag="PK502_03160"
     CDS             complement(4641..5345)
                     /locus_tag="PK502_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPG99847.1"
                     /translation="MGREIIMANKNKKKHWGMVIDLTRCVGCQTCAVGCKIKNNLADG
                     MWWNRVITVGGDHVDTAGGTYNHPMRYSIPLNCQNCENPICVDVCPTGASYQREEDNL
                     VLVDYDKCIGCRACVEACPYGVRLPNHNEPQYEHYDVQLGSDEVTPRYQGVVEKCTFC
                     VERIDQGLNPFCTDVCPERARYFGDLNDEDSDVSKLIRENPVETLHPELGTKPSVYFI
                     PPSKKKTTRTGLDYIK"
     gene            complement(5330..7747)
                     /locus_tag="PK502_03165"
     CDS             complement(5330..7747)
                     /locus_tag="PK502_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPG99848.1"
                     /translation="MSTEIKKFSACVPNCWMNCRLYAHVRDGKLVQTSAAPFPDPSYN
                     RICLRGLSHPQRVNSKDRILHPMKRVGKRGENKFEKITWDEAIDILADNLYKIKEKYG
                     SKAVAFIPWSGSYASINGGMPGAIKRFANIFEGTVVIDGVDFGMPTGMSQVVANMAGG
                     MASLFLGPEPKDLSYAKTIIFWGTNITDSQVHNWHFVLDALENGAKLICIDPRYSETA
                     AKSHEWVRIRPGSDSILALSMIQVIIEENLIDRDYCIQHTVAPFLVRQDNGLFLRQND
                     IDGSDNAGYLTMDADSNELKALETVTNPALLGTFDVTLHNGSKLQVKTALQMLKDEAD
                     KYKPAEAERLTLVPADKIVEIARIYATNTPSTIYTGFGVDRWDNADQVGRGIATLGVI
                     TGQIAKKGASPLGAMGQSAIQYTISDAVMGPWTAPTGTMANLLNFVLLQQAILTGKVD
                     MWEPKDPANPLAGPTGREPVSKDWPIKGLILTTGNLVSNHAEQKVTIDEMFSEDKLDF
                     ICCIDMFMTDTAQQADLFLPCTSWFENDDIVGGLHPYVMRMEKAIEPMGESKGDWDIF
                     KLLAQKMGWGEYFKGDAKDQIDGILDVLGNLFGDHKDKILKEFKENGIARLSDPNALP
                     FADGAYYTRTGRAEFYSEGVATVFPKSMPALQLPVNEGHNPLPHFVPPAEAWIDNPLI
                     KKYPLALATLHTKWRVHTTFHEVPALREMDPEPVVYLNKKESSKRGLKDGDYVKVFND
                     RGHCVVRLVVDDSINDGGAAIPKGWQRHQFKEGGYQELTKNHINTVHYGMSFFDALVE
                     VTKWEGK"
     gene            complement(7769..8701)
                     /locus_tag="PK502_03170"
     CDS             complement(7769..8701)
                     /locus_tag="PK502_03170"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HPG99849.1"
                     /translation="MWTQTPSIIENEQPADRFALHYVPQTHWRIWHAIWFWAMGIGSG
                     IFCLRQFLGFSGGGLFWGMNAIDVIGLVLVAIGGIILILDLGKPFRFLNSLLKPKNAW
                     ISRGAISDFIFLGIGVLYVLPGLTSSLPWSDAVFSEADAFGRILIVLAIFFAFIIMIY
                     PGFVLYYSKNIPFWNSILTPILFLEYALTSSFGLLGLMGALNGDKLPDIFYILFTTTL
                     VLTLLTTVLYILERKSVGNPCIDESIHCLLKGKASGWFWLSQILGIVVPILLLATRSD
                     DFTVLLWSGICTIIGSFIFRYSQLIAGYRVSPLI"
     gene            9062..9892
                     /locus_tag="PK502_03175"
     CDS             9062..9892
                     /locus_tag="PK502_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPG99850.1"
                     /translation="MKSTKVLFISQEIMPYLPETQISKIGRYLPQGIQERGKEIRTFM
                     PRFGLVNERRNQLHEVIRLSGMNLVIEDTDHPLIIKVASIQSARMQVYFIDNDDYFQR
                     KYTTFDCDGKEFPDNDERAIFFCKGVLETVKKLRWTPDLVHCHGWITAFAPILIKKTY
                     AEDPLFAKAKVIYSVYNDEFGTPLDLNVRKKILMEGITKKDVEILKEPSYVNLSKLAI
                     THADAVIVGDRQINPELQEFINTVKKPILPYQNEENYIDVYSEFYDTILNSKTNVKKK
                     "
     gene            9876..11192
                     /locus_tag="PK502_03180"
     CDS             9876..11192
                     /locus_tag="PK502_03180"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HPG99851.1"
                     /translation="MLRRNRPSLILQHIHKPNPGKALIILPLLLISIQSCYNDPNFLG
                     NNLVPEEDIYAVKTDTLFEVSAYSIKTDTILATSGYLGYYNSEIFGSTKGSFVGRYLP
                     TTSTEGFGGPTAKPDSLFFYLKTSSYYGDTLISLAIRLHEITDTAVVWNPTSSLVDLE
                     GKYNPNPLASGSYRGSQVLKIAIDTTFARSLMDSSALANPKEFYTKFKGLYLTCDDLD
                     GFGGVAYYLSTSETYFRLYYHYTKVIDGKDSTISTNKAFTFNSYRFFQYLHDPSKATP
                     SLAVQHLNDTLYQDTVFYVQGMGGVYGKISLDDIAQWKDSVPVAINRAELVIGRVIPQ
                     TVTSDSSLSQLLLYYKDNGVWAGVIPDQISQTTGGVNSNGKLRLYNNDYSIVITSHLQ
                     RILNGEISDKNLYVFPNKTSTFAYSVLQTGNSSRKIKLKLTYTKIR"
     gene            11192..13027
                     /gene="glmS"
                     /locus_tag="PK502_03185"
     CDS             11192..13027
                     /gene="glmS"
                     /locus_tag="PK502_03185"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPG99852.1"
                     /translation="MCGIIGYIGFREAYPVILNGLKRLEYRGYDSAGICVVDGEPFTI
                     KCKGKVSDLENLIGKNSVAGKVGIGHTRWATHGEPNDINAHPHESMNGKFILIHNGII
                     ENYSILKSKLEKRGYTFKSETDTEVLVNLIEYIYQKGNVTPEIAIRLALTKVVGAYGI
                     AVICCDEPETLIAARKGSPLVVGVGEGEYFVASDATPIVEYTNSVIFLNDGDVAILQR
                     DQMVLKTIFNESLEPNIQTLDLDIEAIEKNGFEHFMLKEIYEQPRSIHDTFRGRISLD
                     PIDIHLGGLYNVMPNLVNAKRILMIGCGTSWHAALVGEYVIEDLARIPVEVEYASEFR
                     YRNPIISKDDVVIAISQSGETADTLAAIKLAKEAGALVLGICNVVGSSIPRETHAGVY
                     THAGPEIGVASTKAFTAQVTVLIMMAILLGRKRGTLTEEHYQTLIQEMEKVPEKIEQI
                     LNSDSIVKSISERFKTTNNFLYLGRGYYFPVALEGALKLKEISYIHAEGYPAAEMKHG
                     PIALIDDTMPVVVIATKDNSYEKIVSNIQEVKARKGVIIAIVTEGDTVIREMADYVIE
                     IPEAVEAIGALLAVIPLQLLAYHIALLRGCNVDQPRNLAKSVTVE"
     gene            complement(13139..14194)
                     /gene="rfbB"
                     /locus_tag="PK502_03190"
     CDS             complement(13139..14194)
                     /gene="rfbB"
                     /locus_tag="PK502_03190"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPG99853.1"
                     /translation="MKRNILITGGAGFIGSHVVRRFVKKYPDCLIVNMDKLTYAGNLM
                     NLADVENEPNYRFVKGDIADEHEVEEVFCSYEIDGVIHLAAESHVDRSIADPMSFIKT
                     NIVGTTTLLNAALKSWGHNLQNKLFYHISTDEVYGSLGSEGFFTEQTSYDPRSPYSAS
                     KASSDHLVRAYHHTYGLPTVISNCSNNYGPNQFPEKLIPLTINNIKNKKPIPVYGKGE
                     NIRDWLYVEDHAAAIDLVFHRGEHGKTYNIGGNNEWKNIDLIRLLCRILDDKLDRQKG
                     ESEKLITFVRDRAGHDLRYAIDSSKIRSELGWRPSVSFEEGLERTVDWYLENNAWLEE
                     VVTGQYLTYYEKQYANR"
     gene            14285..14357
                     /locus_tag="PK502_03195"
     tRNA            14285..14357
                     /locus_tag="PK502_03195"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14318..14320,aa:Lys,seq:ctt)
     gene            14471..15139
                     /locus_tag="PK502_03200"
     CDS             14471..15139
                     /locus_tag="PK502_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HPG99854.1"
                     /translation="MGTCIEASTLNFLADLQGNNNREWFQQNRNRYELAFSNFYQLVL
                     SLIAGIGSFDKNLGSPDPKDCIFRIYRDVRFSPNKMPYKQNFGAYIAKGGKKSNLAGY
                     YIHIEPHESFAAGGIYMAPSDIMRRVREDIYDYPEDFLSIVNSKEFRGKFSFEDAESL
                     GKVPRGFEAGTAVDEYLKLKNLIPSISFSDSSIQKETFVDEVLQVYKALQPLVGFINQ
                     AINQ"
     gene            15156..15905
                     /locus_tag="PK502_03205"
     CDS             15156..15905
                     /locus_tag="PK502_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HPG99855.1"
                     /translation="MRGMLAFLFTSLLIVRVGYAQVGFTQEGVASFYGDEFEGRVTAS
                     GERYSHNKATCAHLSIPFGSLVKITNLDNGQTAIVRVNDRGPFVPDRVVDVSRSVAER
                     LGMIPTGVARVKVEVVDANAPLPANQNAIEEVPAKPKVGTVKVAGSSSTSKTSTAQGE
                     GEFYQLKVTPVKPDQYGIQIGSYKEPVNVFKIASDFQKLLKQSMMINVVNVNGEKVYR
                     LLVGGFETRKEAEEVKNKIQNDYPGCFVVGF"
     gene            16008..16709
                     /locus_tag="PK502_03210"
     CDS             16008..16709
                     /locus_tag="PK502_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPG99856.1"
                     /translation="MKIGTLVNDFINLFYPNICAVCHDGLARGEEVICTQCLYHIPRT
                     RYWLETDNPVARLFWGRVNIENACSLFLFAKGSKYRKLIHQLKYNGREDIGVFLGREF
                     GKELCRAELYKSVNFIIPVPLHPRRQKERGYNQAEVIANGLSKSMGIPVIPDVLVRRV
                     YTQTQTKKSRTERVQNVSGAFDLQNPEKISKHHVLLVDDVITTGSTLEVCAQTLLRVP
                     EIKVSVGTLAYASSW"
     gene            complement(16830..18002)
                     /locus_tag="PK502_03215"
     CDS             complement(16830..18002)
                     /locus_tag="PK502_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HPG99857.1"
                     /translation="MKRISITLMLLAPVIAGNAQQVTEAVSMFAFDLYAKTAAGNSSN
                     FVFSPLSITPAFGMVALGARGETQKQLDEVFHFQPTTNFHSELGKLQQKVLKSAGDSV
                     EICMANRAWLEKSYRVLGKYSKALRRSYSAKIATADFVGNFEASRLLINKTVEADTRS
                     YIKNLLPEGSLDEMTRLVLTNAIYFKGKWEVPFDPKRTSARNFNLSSGETVKCPTMLA
                     DDKFGYYVGTNYQALELNYRGNKLSLLILLPNEDTPMADFERNLSLQLYQQATKGLNP
                     EKVTVLLPKFTIETSLSLKQTLSDMGMPIAFSNAANFSGISGKDDLKISDAFHKAYIE
                     VSEEGTTAAAATAVVVAVKSMLPANTFVANRPFIYILRHKDSGAILFMGKVENPLR"
     gene            complement(18135..18440)
                     /locus_tag="PK502_03220"
     CDS             complement(18135..18440)
                     /locus_tag="PK502_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99858.1"
                     /translation="MKMIYCTCNVAVLESLLTMLDENNVRDFQVVEQVTAKNKKGNHR
                     FNNPVWPGYNSTVFIQIAEDEKAKMVMQKIREFNQKAFNDNELVTACMWTIEDYFFE"
     gene            complement(18480..21608)
                     /locus_tag="PK502_03225"
     CDS             complement(18480..21608)
                     /locus_tag="PK502_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPG99859.1"
                     /translation="MKLPEVAVKRPVAITMLFIAVLLFGVVSLTRLPLDIMPDMELPT
                     LTVITVYPGASASEVEQQITKELEMILSGTENLKNISSQSKENVSFVQLQFDWGADIT
                     SAANNVRDLIELVKNKLPREAQNPTIFKVNSSLMPVLAYSIGAKESYGALNKLVNDEI
                     ASKLRKVSGVGSVIIIGEPEREIKINVNPLKLNAYNLSMSQIANILQASNLSIPGGNI
                     KIGSSDYAVQIPGDVASIDELAEMPLTSFNGKVIKLSDVADIHDEFKEKDEYPRTSSG
                     KGLIMMIQKQSGENTLNVANAIRLKVDELKKTLPADVTMSEVMKSDELVSESISNLSE
                     TLWYALFFVILVVLAFLRNWNQSFIIFITIPFSLIVAFIVMFAAGWTINIFSLMSLIV
                     VSGMVVDDAIVVLENITRHVENGEKPKQAAIFGTSEMGLAISASTFTTIVVFLPMVFM
                     GGIVGIMFKQLAILTSVTLLASLFTALTLTPMASSLLVKGIKPGEVHRHNRFFTASEK
                     LLTGLEGWYKKALAWVVYHKAITVIIVTIILGLSVWMGRNMGTDYIPEFDAGDVVAVV
                     ETEVGTAAAKTDSVANVVLKIFEEEIPEMAPGSIFSIAGQTEDGILSSVGFSEGKNVA
                     TIMCHLRKPNERSASAREIANRLRPRISEIPEIENFTLLGGSVLTTALTGNKKPIEIE
                     ISGNNFSTINQTAEALTAKLKQSDYFTNVDNDVDKGKLEVQVKIDKQKASAMGLNAAM
                     IGLQVRQSIYGTEAGEYKEDGDKFDITLRYSPSYRNDINQIRNIQLKNLLNQDIPLYA
                     VAQIETGTSPLQINRKEQQRLVKVMAELNGVSLGDGQMEAQRIVSSTSFPNDVDVKIA
                     GQTSDQGESFADLYLILILGILLVFMVMAAQFESFKDPFIIMFAIPVTFVGVVWAFKL
                     TGLTLSIVTFVGMIMLVGIVVKNGIVLVDYTNLLRKRGYSLYEAIQEAGRSRLRPVLM
                     TTLTTILGMVPMALSKGMGREAYSPLGVTMIGGLLIATLITLFLVPAIYGIFHNSERK
                     SNGVAKKIDYSR"
     gene            complement(21780..22826)
                     /locus_tag="PK502_03230"
     CDS             complement(21780..22826)
                     /locus_tag="PK502_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPG99860.1"
                     /translation="MKPTILGLTPLAIALVLTGCKTSKTEVPATEENVPLVSTVVVEE
                     RIYSPTLSFTGTAFANREANLGATLPGKVEKIYFDEGQNVHEGDLIVELSDELLTQTI
                     IENETIKKDFDRVSRLREKGSISEMEYDHVKAQYDASQAKVQMVKKNTRVYAPFSGMV
                     VERMMEEGEVYFLNPGLEPGYSMRSGIVRLMQLNPVKVRFDVNEKDLGVIRKGLEVEV
                     TFDGVAGKTYQGKVGTIKQILSTTTHSAAVEVTISNPQLEIKPGMFAYITVKLPEIKA
                     SVVPLKSIYRLPGTSDDYLFSVTNDTINRIPISRLESIGEYVAVKGIEPGVEVVLDGK
                     TRVSQGQRVRVSGK"
     gene            complement(22877..>23081)
                     /locus_tag="PK502_03235"
     CDS             complement(22877..>23081)
                     /locus_tag="PK502_03235"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPG99861.1"
                     /translation="VQMAEENLKQAEENLKVTRNAFDEGKLKTSDVLEAQTLWQNAYS
                     DLIDARMEYRLNITNLKKVTGTL"
BASE COUNT         6292 a         5055 c         5044 g         6590 t
ORIGIN      
        1 atttgtaaag ttaactgatt atcaatgtta acgattccca atctttttct tgtcggcggc
       61 aattctcgcc attctggcaa aacgacttta gcgtgtgaga ttattcgtcg attggcgaag
      121 gagcaggagg ttaccgctat caaattcata cgatttgatc ctgatgaaaa agagatgcat
      181 ggcagccatt tgggagaaag tgaattttcg ggttattcca tcatgcgaga aaccaacaat
      241 cattcatcga aggacacttc tcaaatgttg caagcaggag cgaaagaagc gttttacatt
      301 cgctcgggag aagaatttat ggagcaggcc tcgattgatt ttttaacgag atataatcac
      361 ggtcaacctt tggtgtgtga gagcagcgct ttgcgccatt ttgtcgaacc ggccgttttt
      421 gtcatgatga tgcgaattcc gcccatcggg aatcctaaac ctgtagaaag ttatttacca
      481 ctagccgatc gaattttgta tttcgacaaa agttatgacg aaagattgcg aatgcttgag
      541 gaattgcgat ttgatagcgg aaagttttat cttccttctt tcaagtaggc tgtaatttct
      601 tgaatcagta tttctgggtt acattctgcc acattgtaaa ttgctgccgg atgaagactc
      661 catttttcga tgacgtcttc cacaaattcc tgtcgtattg atagcaatat atacaattgt
      721 ttctcgagag gcaacaaatt caattcgttg tgccatccgc cgccgctcat ttccaatttg
      781 ccgatttcgt caatcatcaa taccctcgaa tcaaacgatt tcactatatt ttttttgcca
      841 aattcgagtc cctcagcatc aattgaaaaa gccccaatac gcaattccga ctccaattca
      901 tttttctgca agaaaggata ttcttcgccg gttgcaacgt ttacaatcag ataagctgta
      961 atttcatcgt tttccctgat tttttttgta taaacacctc ctacgtcaat tccattttct
     1021 ctaagcgttg ttgctgtctc tgtcaaaaat ttggttttgc cactgctttt ttcgccggta
     1081 attattgcta ttgctgcttt atgttgtttt cgcgattcta tttcatgcat ccggtattcg
     1141 gcatacgaca acaattgttt tacgatttcg agtggatgtt tgaagatctc tttcagattg
     1201 ggaacaagcg caatcatatt cggaaggctc tcggccgaaa ggtcaagagc catatatagc
     1261 gagatcaagg cttttttttg aaagagattt ttaattttag gactgtaaag ttctgtccct
     1321 attaccgcaa aaccgatcac cataactatg gcacgaacat tcatttccaa tccaatgagt
     1381 gttccttgcc acaacgaaat acccgactga aattgtgaaa aagcaatggc ggcaatcacg
     1441 gtgatgataa aaaacgaaat ccacaattta ggttggccca aacgtcgcag ggcaacttta
     1501 taatggaaaa tccaacaaac tacgacaaga atgctgattc caaaccaaag aatgacatta
     1561 attcttttgg caagtaccag cactaaaatg attgatagaa catcggcaat aagccatgct
     1621 gttgaaaaag ggaaacgagt atattttgaa gttggcaaaa gcgatgcttt gtttttcgct
     1681 ttgaatattt cagcattcaa atcggttgat ccaagttttt tcccaatgaa aatgcctgca
     1741 atagctgttc caatacctaa aagaatataa atggtcaaca acaaaattat cggagcccaa
     1801 accatatcta tattggtatt cagctgtttt tgaacatatt gcatgatgct ttcatacaat
     1861 tttaccaaat tgtatccgta agcaattact ttgctgataa taaactgaac cagattccac
     1921 gacatggcta aaatagctcc tacggtaaat ccgattctgt tttttcctaa taagctcact
     1981 gccgcttcaa gcaaaattgc ttcactgaga atggcaatca tcggccctag tataatagca
     2041 cttggcgaca tcgttttcag caaagcacag atgacgcctg cccgccaaaa caaacccttt
     2101 tctctccagc gataacttac agaaataagg ataatcaacc caatggctgt gagaatatgc
     2161 ccgctgaatg gaactctcag attatgcaga aaactgcccc atacaatttc caccgaagcc
     2221 cacatcgagc caagtatgga tgctttaatc catttttcgt tcagggttgt ttgcgattgt
     2281 ttcacaagat tgaattttga aaacggtttt ttaatttaga tgaaatagaa gtttggtaaa
     2341 gtaatttctt ccaatttcct gataacctta ggcgtaagcg taattcgcat cgaaaaggtt
     2401 tttaatgcca gcaaaaagtg tgaactgttt cctccaagcc gaaccttgta agttgaacag
     2461 atgaaactca ttggtaacaa tgccacagga aggatttgaa tttttttttt gaatgataaa
     2521 ggaatatgat gccgtcaaat cgagattata cagcaatgca aaatgcaagc tgaggtctgc
     2581 accattaaaa cgcgatttcc ctgcattctg aagctggatg aggtttccct gaacgccaca
     2641 ctccgattta atgacatcat tcagccgaat atggtgtatt gaaccatcga tgggaagatt
     2701 ccttaaaaga ttgtattgaa acgaaatgta ggaatgagcg gcttttacag actgaaaatt
     2761 cagattgaaa agttcttgtt ccattttgta cgaatgtctt tcccttaggg ttgtaaaacg
     2821 tgttttttga gcaacaaatg ccgaaacgga taatttgtga ttcggcatcc aactcatagc
     2881 tatctgtgca tttgcagcat gatgattatt tttcgggaaa ttagtcgtat cggcagctgt
     2941 aacctgatca tcagcttcga ggctttttag atagttgtag ggcaatcggg cagtgaagtc
     3001 gattttatcg ttgagtcggc atttatcgtc cactcctgtg ctgcaagtaa aatcgctgtt
     3061 cataatcttc gcattttcca ctttgtgcaa tttgtgctcg tttcggttca tttgaatcga
     3121 ataattttgc tgattgttcg ttatcgaagt atttnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnncaaaat tccgatggtt gttcatgctt gtaaacgcat attttttggt
     3301 ttgacttgta tgggtttaat catcaaagct tttcattacg ttgtataatt tgtcggcgta
     3361 aagacttttg atgctgtttt cattgttgat tttattgttt gcaataaaat ataaacgctg
     3421 atgttccgat accggctatt gccagaatcg tgttgattgg gttttattgc ttccactgta
     3481 aggttgactt ccctttgcat tgtactgatt tagataattg tgcgtaaatt attgatcggg
     3541 agcagcaata tatccgattt tgaagtttcc ctttctgaaa atcgttgttg ataataactc
     3601 gattgaaaga aaaacatttt ccatcggctt cctacgttca ctccatattc gtaggtgttt
     3661 ccgcttgcca atccagcaat tacctgtaaa tttagcttat cagttggctg aaaacttatc
     3721 aaattgatgc ttccgtcggt agcattcata cccgacgaca tgggggtgga acccttggtt
     3781 aatgaaacaa gcgaataatc gaacgtagcg agttgtccca tatcggtgtc cccgttgtaa
     3841 aacttttgta gtgaaagccc gtcaacaaaa acccgaatgc cgtgaatatc gaatccgcta
     3901 actactaccg taggttcaaa acgtccgccc atattggcaa atatgacgga tgaaacagta
     3961 cacaatgctt cgcttatgtt cgtggtgttc atttgcaaat gaattgtttg ctctaccttt
     4021 tctctcattt ttggggctga taaaacgaat tcgcccaatt ggaaggtttt tgatattttg
     4081 gtagcgtcgg tttctgccgc ttttatggta gtgatgaata tcgtgagagc aatcaatgtt
     4141 aagagatgct gtttcataag atgttctgta ttgtcgttat tcctaaaatg aataacggga
     4201 tgtaaaaaat ttagtgtaca gcttcaaatt tgcaattttg aataaagtcg gtcaattgtt
     4261 tttctattat ctcgtattct ttcatgagtt tttttccata agttgttatt tctgacactc
     4321 ccccattctt tccacctctt ttcaactcca caacaggttg tttggcggct ttattcatcg
     4381 aataaattcg ataataagcc ttccgatacg agatgcgcaa ctcttcggct gcctgtcgta
     4441 gcgatccgcg ctggtcaatc ttacgaagca aatccatttt gcctttcccg agcaaaggtt
     4501 ttccgtcgaa tgaaacccaa aatctgaatt ctatctgaaa attcgagttc aacttttcaa
     4561 aattattttg tttagggaaa taaaaaggta aacacaagac ttttctttac cttgtgttta
     4621 cctttttcag caaaatgatc ttatttgata taatccaatc ccgttcgggt ggtttttttc
     4681 ttacttggag gaatgaaata aacgctcggt tttgttccca attcgggatg aagtgtttca
     4741 accggatttt cgcgaatgag tttcgagaca tcactatcct catcgtttaa atcgccaaaa
     4801 taacgagcac gttcaggaca aacatcagtg cagaaaggat tcaaaccctg atcgatacgt
     4861 tccacacaaa atgtacattt ttccactacg ccctgatatc tcggcgtaac ctcatccgaa
     4921 ccgagttgca catcgtagtg ctcatattgc ggctcattat ggtttggcag gcgtactcca
     4981 tatggacatg cttccacaca ggcacggcat ccgatacatt tgtcgtaatc caccagaacc
     5041 aaattgtcct cttctctttg gtaggatgct ccggtcggac aaacatccac acaaatcgga
     5101 ttttcgcaat tctgacaatt caaaggaatg gaataacgca tcggatgatt gtaggttcct
     5161 ccggcagtat ccacatgatc gcctcctacg gtgataactc tgttccacca catgccatca
     5221 gccaaattat tttttatctt gcatccgact gcacatgttt gacaacccac acagcgggtc
     5281 aggtcaatca ccattcccca atgttttttc ttgtttttat ttgccataat tatttccctt
     5341 cccattttgt tacttctacc aaggcatcga aaaacgacat gccgtagtga actgtattga
     5401 tatgattttt agttaattcc tgatatccac cttctttgaa ttgatgacgc tgccaacctt
     5461 tgggaattgc agcacctcca tcgttgatgg aatcgtccac cacgagtcgt acaacgcaat
     5521 gaccacggtc gttaaatact ttaacgtaat cgccatcttt aaggcctctt ttactcgatt
     5581 cctttttatt gagatagacc accggttcgg gatccatttc ccgtaacgcc ggaacctcat
     5641 gaaaagtagt atgcacacgc cattttgtat gtagcgtagc taaggcgagc ggatattttt
     5701 ttatcagcgg attatcaatc catgcttctg ccggtggcac aaaatgaggc aatggattat
     5761 gcccttcgtt gacaggcagc tgcaatgctg gcatactttt ggggaataca gttgccacac
     5821 cttccgaata aaattcggcc ctgcctgttc gcgtatagta agcgccatcg gcgaatggaa
     5881 gtgcattcgg atcggagagc cgcgctattc cattttcctt aaactctttc aatatcttat
     5941 ctttatgatc tccaaaaaga ttgcccaata catcgaggat tccgtcgatt tgatcttttg
     6001 cgtctccctt gaaatattct ccccaaccca ttttttgagc taacagcttg aagatatccc
     6061 aatcgccttt actttcgccc attggttcaa tggctttctc catccgcata acataaggat
     6121 gaagtcctcc tacaatatcg tcgttttcga accagctggt acaaggcagg aacaaatcgg
     6181 cctgttgagc cgtatcggtc atgaacatat caatgcagca aataaaatcg agtttatctt
     6241 cgctgaacat ttcatcgatg gtaactttct gctcggcatg attgctcacc agattgccgg
     6301 tagtcaaaat caaaccttta atcggccaat ctttcgaaac gggttctctt cctgtaggcc
     6361 ctgcaagagg attggcagga tctttgggct cccacatatc caccttccct gtaagaatag
     6421 cttgttgcag caatacgaag ttcaacaaat tggccattgt tcctgttgga gcagtccatg
     6481 gtcccatcac agcatcggaa atagtatatt gaatggcact ttgacccatt gccccgagag
     6541 gagaagctcc ttttttggca atttggccag tgataactcc taatgttgca atgcctctac
     6601 ccacctgatc ggcattatcc caacgatcaa ctccaaatcc ggtatagatg gttgaaggcg
     6661 tattggttgc ataaattcga gcaatttcta caatcttatc agcagggaca agtgtcaacc
     6721 tttcggcttc agccggtttg tatttgtccg cttcatcctt caacatttgc aaggctgttt
     6781 ttacctgcaa ttttgatccg ttgtgtagcg taacgtcgaa agtccccaat aaggcaggat
     6841 ttgtaaccgt ttccaatgct ttaagttcat tcgaatcggc atccattgtc aggtatccgg
     6901 cattatcgct gccgtcgata tcgttttgac gcaggaaaag tccattatcc tgacgaacaa
     6961 ggaatggggc aacggtgtgt tgaatgcaat aatctcggtc gatgaggttt tcttcgatga
     7021 ttacctgaat catggataaa gctaaaatgg aatccgatcc tggacgaatg cgaacccatt
     7081 cgtgcgattt ggctgccgtt tcgctgtaac ggggatcaat gcaaatcagt tttgcaccgt
     7141 tctccaatgc atcgagcacg aaatgccagt tatgcacttg cgagtcggta atgtttgttc
     7201 cccaaaagat gatggttttg gcatagctca aatctttggg ttcaggtcct aaaaagagcg
     7261 aagccattcc accggccatg ttggcaacta cttgcgacat gccggttggc attccgaaat
     7321 cgacaccatc tattacaacc gtaccttcaa aaatgttggc aaagcgttta atggcgcctg
     7381 gcatacctcc attgatagaa gcataactac cggaccaggg aataaacgca actgcttttg
     7441 aaccatactt ttctttgatt ttatataaat tgtcagccaa aatatcgatg gcttcgtccc
     7501 acgtgatttt ttcgaatttg ttttcgccac gtttccctac acgtttcatc gggtgaagga
     7561 tacggtcttt gctgttgacc cgttgtgggt gcgataatcc tcgcaggcaa atacgattgt
     7621 aggaaggatc gggaaaagga gcagccgatg tctgaaccag ttttccgtct cgaacgtgag
     7681 catataatcg gcagttcatc caacagttgg gaacgcatgc cgagaatttt tttatttctg
     7741 tactcattcc agctattttt tccaattatt aaattaaagg tgaaacgcga taaccggcaa
     7801 tcaattgact gtagcggaat atgaaacttc caataatagt gcagatgccg ctccaaagaa
     7861 gtactgtaaa atcatcactc cttgtggcta ataacagaat gggaacaaca attcctagta
     7921 tttgcgaaag ccaaaaccat ccggaggctt tacctttcaa caaacaatga atggattcat
     7981 caatgcatgg attcccgaca cttttgcgtt ctagaatata aagaacagtt gttaacaacg
     8041 ttaacacaag agttgtagta aataaaatgt agaaaatatc gggaagctta tctccattca
     8101 aagctcccat caatcccagc aatccaaagc tgctcgtaag agcatattct aaaaataaaa
     8161 ttggcgtcaa aatgctattc cagaagggaa tgtttttaga ataataaagc acaaatccgg
     8221 gataaatcat aataatgaaa gcaaagaaaa tggccagaac gatgagaatc cgaccgaaag
     8281 catcggcttc tgagaatact gcatccgacc aaggtagcga agaagttagt cctggaagga
     8341 cgtaaagaac accaattcca agaaaaataa agtccgaaat cgctcctcgc gaaatccatg
     8401 catttttagg ttttagtaat gaatttaaaa atcgaaaagg cttacctaaa tcaaggatca
     8461 gaataattcc accaatagcc actaaaacca atccgatgac atcaatggca ttcatccccc
     8521 aaaatagtcc tcctccgctg aaccctaaaa actgccttaa acaaaaaatg ccacttccga
     8581 tgcccattgc ccaaaaccaa atggcatgcc aaatgcgcca atgggtttga ggaacatagt
     8641 gaagcgcaaa tcggtcagca ggttgttcgt tttcaatgat actgggtgtt tgtgtccaca
     8701 tagttagaat ttattttgtt gttaattgat atctgattga aattaaaact gacactcctt
     8761 ttatatctga attaattcat caaatgtata aatatattta tgaataatca taaatttgat
     8821 aaggaaaatt cttaatatca tgtaaattta aattagaaaa aacctgcaaa gaaagtcggt
     8881 ttttttttat atttttgtaa gattttattg ttgagaagcc cggtttcgga cttagtagct
     8941 gaagccgggt tttaccagta ttcctaaggt aggtgcaaga ttcagtgtaa gagatctatg
     9001 atgcatagga ttatttgcat caccagcagg ggaggtgaag ttaaacttaa aagtaaaaac
     9061 cgtgaaaagc accaaggtac tgtttatctc gcaggaaatc atgccctacc tgcctgaaac
     9121 ccagatatcc aaaataggtc gctatttgcc tcagggtatt caggagcggg gaaaggagat
     9181 acgaaccttt atgccaaggt ttgggctggt aaacgaacga cggaaccagc tgcacgaggt
     9241 tatccggctt tccgggatga acctagtaat tgaggatacc gatcatcccc tgataatcaa
     9301 ggttgcctct attcagagcg cacgcatgca ggtttacttc attgacaatg acgactactt
     9361 tcagcgaaaa tataccacct tcgactgtga tggtaaggag tttcctgaca acgacgaaag
     9421 ggcaattttc ttctgtaagg gtgtactcga aacggttaaa aagttgcgct ggactcccga
     9481 tttggttcat tgccatggct ggataactgc atttgcaccc attctcataa aaaaaaccta
     9541 tgccgaagac cccctgtttg ccaaggcaaa agtcatttat tcagtttaca acgacgagtt
     9601 tggcacgccg cttgatttga atgtgcgtaa gaaaattttg atggagggca ttaccaaaaa
     9661 ggatgtggaa attttaaaag agccatccta tgttaacctg agtaagctgg caattaccca
     9721 tgcggatgcc gttattgtcg gcgataggca aattaacccc gagttacagg aatttataaa
     9781 tactgttaaa aagcccatcc tgccttacca aaatgaggaa aattatattg atgtttacag
     9841 cgaattttat gataccatcc ttaatagcaa aacaaatgtt aagaagaaat agacctagtt
     9901 taatattgca acatatccat aagccaaacc ccggcaaagc gcttatcatt ttacccttgc
     9961 ttttaatatc cattcaatcg tgctacaacg atcccaattt tctgggcaac aaccttgtac
    10021 ccgaagagga tatttacgct gtaaaaaccg atacgctatt tgaagtttct gcctactcaa
    10081 taaagacaga caccattctt gccacaagcg gttatttagg ctactacaat agcgaaattt
    10141 ttggctccac caaggggagc tttgtgggta gatacctgcc aacaacctcc accgagggtt
    10201 ttggagggcc aaccgccaag ccagattccc ttttcttcta cctgaagacc tcatcgtact
    10261 atggtgacac tctcattagt ctggcaatca ggttacacga aattaccgat acggcggttg
    10321 tttggaaccc caccagcagc ctggtggacc ttgagggaaa gtataacccg aaccccttgg
    10381 caagcggctc atacagggga agtcaagttt taaaaattgc aattgatacc actttcgcgc
    10441 gcagcctgat ggactcatcg gcactggcta acccaaagga attttatacc aaattcaagg
    10501 gtctttatct aacctgcgac gatcttgatg gttttggagg cgttgcctac tacctttcaa
    10561 catccgaaac ctatttcagg ctttactatc attacacaaa ggttatagat ggtaaggatt
    10621 ctaccattag caccaacaag gcctttacat tcaactcata ccgttttttc cagtaccttc
    10681 acgatccctc aaaagcaacc ccatcgttag ccgttcagca tttaaacgac acgctttacc
    10741 aggacaccgt gttctatgtt cagggtatgg gcggggtgta tggaaaaatt agccttgacg
    10801 atattgcaca atggaaggat tcagtacccg ttgctataaa tagggctgag ctggttattg
    10861 ggcgggtaat tcctcaaacg gttacatcgg actcatcgtt aagccaactc ctcctttact
    10921 ataaggataa tggggtatgg gcaggtgtaa tccccgacca gattagccaa actactggcg
    10981 gggtaaacag caatgggaag ctgaggcttt acaacaacga ctacagcatt gtaattacct
    11041 cccatcttca gagaatcctt aacggggaaa tctccgataa aaatctgtac gtattcccca
    11101 acaagaccag cacttttgcc tactctgtgc tccaaacagg gaacagcagc cgaaaaatca
    11161 agttgaagtt aacctataca aaaataaggt aatgtgtggt ataattggct atattgggtt
    11221 tagggaggcc tatcccgtta tacttaacgg acttaagcgt ttggagtatc ggggctacga
    11281 ttcggccggt atatgtgtgg ttgatggtga accgttcact ataaagtgca agggaaaggt
    11341 ttccgacttg gaaaacctca ttgggaagaa ttctgttgct ggaaaagttg gaatagggca
    11401 cacccggtgg gcaacccatg gtgagccaaa cgatattaac gctcacccac atgagtcgat
    11461 gaacggaaag ttcatcctta tccataacgg gataatagag aactacagca tacttaagag
    11521 caagctggaa aagcggggct acacttttaa atcggaaacc gacactgagg tgctggtcaa
    11581 ccttattgag tacatctacc agaagggtaa tgtaacccct gaaattgcta ttcgcttggc
    11641 attaacaaag gttgtaggtg cttatggaat agcggtaatt tgctgcgatg aacccgaaac
    11701 tctcattgct gcacgtaagg gaagtcctct tgttgttggc gttggcgagg gtgagtactt
    11761 tgttgcttcc gatgccactc caattgtgga gtacaccaac agcgtaattt ttctcaacga
    11821 cggggatgtg gctattttac aacgagacca gatggtgcta aaaaccatat ttaacgaatc
    11881 gctggagccc aacattcaaa cccttgacct cgatattgag gccatagaaa aaaatggctt
    11941 cgagcatttc atgctaaagg agatatacga gcaaccgcgt agcattcatg atacctttag
    12001 agggagaatc tctctggacc ctattgatat tcaccttgga ggattgtaca acgtaatgcc
    12061 caacctggtt aatgctaagc gcattctgat gataggttgc gggacatcgt ggcacgccgc
    12121 actggtaggc gaatacgtta ttgaagattt agctagaatt cccgttgagg tggagtatgc
    12181 ctcggagttt agataccgaa accccatcat cagcaaggac gatgtggtga ttgccatcag
    12241 ccagagcggc gaaactgccg ataccctagc tgccattaaa cttgctaagg aagcaggagc
    12301 cttggtgctg ggtatttgca acgtggtggg ttccagcata ccccgcgaaa cccatgccgg
    12361 cgtttacacc catgccggcc ccgaaatagg ggttgcctct actaaagctt tcaccgctca
    12421 ggttaccgtt ctaattatga tggccatttt gctgggacgc aaaaggggaa cacttaccga
    12481 ggagcactac cagacattaa ttcaggaaat ggagaaggtc cccgaaaaaa ttgagcagat
    12541 acttaactcc gattcaattg ttaaaagcat ttccgagaga tttaagacaa ccaataactt
    12601 cctgtacctt ggcagaggct actacttccc cgtggccctt gagggagcgc ttaagctgaa
    12661 ggagatatcc tacattcatg ctgaggggta tccagctgca gagatgaagc atgggccaat
    12721 agcgctaatt gacgatacga tgcccgttgt ggttattgcc actaaggaca acagctatga
    12781 aaagattgtg agcaacatcc aggaggttaa agcacgaaag ggcgtaatca tcgcaatagt
    12841 taccgaagga gacacggtaa tcagggaaat ggccgattac gtgatcgaaa ttcccgaagc
    12901 cgttgaggca attggcgccc tgttggctgt tattccattg cagctgctag cctaccacat
    12961 tgccctgctt aggggttgca atgttgacca gccacgcaac ctggccaaat cggttacggt
    13021 ggagtagaat tttcagaaaa atactccaga aattgtttca ggcacatttt aaaattaaaa
    13081 aggagatggc tttctatctc cttttttatg tttgtatgtt tagtcaagca ctaaagggct
    13141 acctgttggc atactgtttc tcgtaatagg ttaagtattg gcctgtaaca acttcctcta
    13201 gccaggcgtt gttttccaga taccaatcca cggttcgctc aagcccctcc tcaaagctaa
    13261 cggatggtcg ccatccaagc tcgctacgga tcttgctgga gtctattgca tacctcaagt
    13321 cgtgtccagc cctatccctg acaaaggtaa tcagcttttc agactctcct ttttgcctgt
    13381 ccagcttatc atccaaaatc cgacaaagca atcggatgag atcaatattc ttccactcgt
    13441 tgtttcctcc aatattatac gttttaccgt gttcgcccct atgaaaaacg aggtcaatgg
    13501 cagcggcatg gtcttcaacg taaagccagt cgcgaatatt ttcacctttc ccgtaaaccg
    13561 gaatgggttt tttgttttta atgttgttta ttgtgagcgg aatcaacttc tccgggaact
    13621 ggttgggacc gtagttattg gagcagtttg aaataacggt tggcaacccg taggtatggt
    13681 ggtaggctcg taccaggtgg tcggaagagg ccttcgaggc ggagtaaggg ctacgtggat
    13741 cgtatgatgt ttgctcggta aagaatcctt cgctgcctaa gctgccgtaa acctcatcgg
    13801 ttgaaatgtg gtagaacagc ttattttgta ggttgtgtcc ccagctcttc agggcagcat
    13861 tcaaaagggt tgtagttcca acaatgttgg ttttaatgaa cgacatgggg tctgctatgg
    13921 agcggtcaac atgcgactcg gctgccaggt gtattacgcc atcaatctcg tagctacaaa
    13981 aaacttcctc aacctcgtgc tcatcggcta tatcaccctt cacaaaacgg tagttaggct
    14041 cgttttcaac atcggcgagg ttcatcaggt taccggcata ggttagctta tccatgttca
    14101 caatcaggca gtcgggatac ttctttacaa aacgcctaac tacatgtgaa cctatgaaac
    14161 ctgcgcctcc ggtaatcaga atatttctct tcattacgtt aaaatttcag gtaaagtaag
    14221 caataataat tttcaatttt ttggataagg tgaaaaatac ctataaattt gccaccacaa
    14281 aaatcggttc gtagctcagc tggtagagca cgtgactctt aatcatgggg tcgagggttc
    14341 gagcccctcc gaaccgacag cgccacctta aggtggcttt ttgttttaaa ccatttccac
    14401 caaccaattg cagggagctc cattcgctac agtttgcaaa ttttattaat tttggtaaaa
    14461 attatgctgt atgggtacct gcatcgaagc ttcaacctta aacttcttgg ctgatttaca
    14521 aggaaataac aatcgtgagt ggtttcagca aaatcgaaac cgttacgaat tggccttcag
    14581 taatttttac cagctggtgc tgtcgttgat tgccggtatt ggctcttttg ataaaaactt
    14641 gggatcgccc gatcctaagg attgcatttt cagaatatac cgcgacgtac gtttttcacc
    14701 taacaagatg ccctacaagc aaaattttgg ggcatatatt gccaaaggag ggaaaaagag
    14761 taacctggcc ggctactata ttcatattga gccacacgaa tcatttgccg ccggtggaat
    14821 atacatggcg ccttccgaca tcatgcggag agttagggag gatatttatg attacccgga
    14881 agattttttg tcaattgtaa attcaaagga gtttagaggg aaattttcct ttgaggatgc
    14941 ggaaagcctt ggaaaagttc ccagaggttt tgaagccgga acagcagttg acgagtactt
    15001 aaagcttaaa aaccttatac cctccatttc attcagcgat agcagcatac agaaggagac
    15061 ttttgtggac gaggtgctgc aggtatataa agcgctgcag ccacttgtgg gtttcataaa
    15121 tcaagccata aaccagtaaa gtataaaata tgagtatgag gggaatgctt gcttttcttt
    15181 ttacctcact tttaatagta agggttggat atgcccaggt tggcttcacc caggagggag
    15241 ttgcatcttt ttacggcgat gagtttgaag gcagggtaac cgcttcgggc gaaaggtaca
    15301 gccacaataa ggctacctgt gcgcacctgt ccattccgtt tggctcgctg gtgaaaatta
    15361 ccaatttgga taatggacaa acagcaattg ttagggttaa cgacaggggg ccttttgtgc
    15421 ccgacagggt ggttgacgtt agccgttcgg ttgccgaaag gctgggtatg attccaaccg
    15481 gtgtagcccg ggttaaagtt gaagtggttg atgcaaatgc gcccctgcca gccaaccaga
    15541 atgcgattga ggaagtaccc gcaaaaccta aggttggtac tgttaaggtg gcaggctcat
    15601 ccagcacgtc caaaacttcc acggctcaag gcgaggggga gttttaccaa cttaaggtta
    15661 caccagtgaa gcctgaccaa tatggcattc aaattggcag ctacaaggag ccggttaacg
    15721 tttttaagat tgcaagcgat ttccagaaat tgctgaagca gagcatgatg attaatgtgg
    15781 ttaatgttaa tggcgaaaag gtttaccgcc tgctagtagg tggattcgaa acccggaagg
    15841 aggccgagga ggtaaagaat aaaattcaaa acgattaccc gggctgcttt gtggttgggt
    15901 tttagcatta aaaaatgcgg tgggtagtaa gtaattaggg tattcggcat tttaagagtt
    15961 aatttcctac ctcaggagta ggttgggtta ttgcaaagct taagggaatg aaaataggta
    16021 cgttggtaaa cgattttata aacctgtttt acccgaatat ctgcgccgtg tgccatgacg
    16081 ggttggcaag gggtgaggaa gttatctgca ctcaatgttt ataccatatt cccagaacaa
    16141 gatactggct ggaaaccgat aatcctgtgg cgcggctatt ctgggggagg gtaaatattg
    16201 aaaatgcctg ttcgctattc ctattcgcaa aggggagtaa gtaccgaaag cttattcatc
    16261 agctaaaata caacgggagg gaagacatcg gtgtttttct aggcagggag tttggaaagg
    16321 agctctgcag ggcagagctt tataaatcgg taaatttcat aatacccgta ccgcttcatc
    16381 ccaggaggca gaaggaaagg ggctataacc aggcagaggt gattgccaac gggctttcaa
    16441 agtcaatggg aataccggtt attccggatg tgctggtgcg aagggtttac acccaaaccc
    16501 agaccaaaaa gtcgagaacc gaaagagttc agaacgtttc gggagctttt gaccttcaga
    16561 atcctgaaaa gatttcgaag catcacgttt tgctggttga cgatgtaatt acaacagggt
    16621 caacgctgga ggtgtgcgct cagacacttc tacgagttcc tgaaatcaag gtgagcgttg
    16681 gaacactagc ctacgcttcg tcatggtaaa ctaaatatag ggtaatttga aaaatgcagg
    16741 ggtaaaaatc gccctgcata cttcatacag ccaattcaac cggcaacagt aacttttgcg
    16801 gctcaactgg ccgcttggag gttgaaattc tacctaagtg gattttccac cttgcccata
    16861 aagagaatgg caccggaatc cttatggcga aggatgtaaa taaaaggcct gttagcaaca
    16921 aaggtgttgg ccggtagcat cgatttaacg gctactacaa cagcagtggc agcagcagca
    16981 gttgtaccct cctctgaaac ctcgatgtaa gccttgtgaa acgcatcgga aatcttcagg
    17041 tcatccttgc ctgaaattcc cgaaaagttg gcagcattgc taaaggctat gggcataccc
    17101 atgtcgctaa gcgtttgctt taggctgagg gaggtttcaa tggtgaactt aggcaggagc
    17161 acggtaactt tttcagggtt cagccccttt gtagcctgtt ggtagagttg tagtgaaaga
    17221 tttctctcaa aatcggccat gggtgtatcc tcgttgggca gaaggattag cagcgaaagt
    17281 ttgttcccgc ggtagtttag ctccagtgcc tggtagttgg ttcccacgta gtatccaaac
    17341 ttgtcatcgg caagcatggt aggacatttc accgtttcac ccgatgaaag gttgaagttt
    17401 cttgccgaag ttcgtttggg atcgaacggt acttcccatt tacccttgaa gtagatagca
    17461 ttggttagca ccaggcgggt catctcgtcg aggctacctt ccgggagaag atttttaatg
    17521 tagctccttg tgtcggcttc aacagttttg ttgatcagca accgtgaggc ttcaaaattt
    17581 ccaacaaaat cggcagtggc aattttagcg ctgtatgagc gtcgtagcgc cttgctgtac
    17641 ttgccaagca ccctgtatga tttttcgagc caggcgcggt tggccatgca aatttccacc
    17701 gagtcgccgg ccgattttaa aaccttttgc tggagtttac ccaactcgga atgaaagttg
    17761 gttgttggct ggaagtggaa aacctcatcc agctgttttt gggtttcgcc ccttgcgcct
    17821 agcgcaacca ttccaaaagc aggagtaatg cttaggggag aaaaaacaaa gttgctgctg
    17881 ttcccggcag ctgtttttgc ataaaggtcg aaggcaaaca ttgagacagc ctcagtaacc
    17941 tgctgggcat taccggctat taccggggcc agcaacatca aagtaatgga tatccgcttc
    18001 atggtagtaa ttttttacca agttacaaca aaagttatgc aagtcataag tctgccttgc
    18061 aggaattgtt ttttctctgt gcttcccgca tgttcgccgt agaagtgcca aagttaatgg
    18121 attgctgtat gcttttactc gaaaaagtaa tcctcaatag tccacatgca ggctgtaacc
    18181 agctcgttat cgttaaatgc tttttggttg aactcccgta ttttttgcat caccattttt
    18241 gccttttcat cctctgcaat ctggataaaa actgtagagt tatagccagg ccaaacgggg
    18301 ttgttgaacc ggtggttacc ctttttgttt tttgccgtaa cctgttccac cacctggaag
    18361 tcgcgcacgt tgttttcgtc cagcatggtt agtagcgatt ccaaaacggc aacgttacag
    18421 gtgcagtaaa tcattttcat gtgttgcagc tattagtgaa attaacatga agctataaac
    18481 tatctggaat agtcaatttt tttagctact ccatttgatt ttctttctga attgtggaaa
    18541 attccataga tagccggaac aaggaacagg gtaatcagcg ttgcaatcag cagaccgcca
    18601 atcatggtaa cgccaagggg tgagtaagcc tcgcgcccca tacccttgct caaggccatg
    18661 ggcaccattc caaggattgt ggttaaggta gtcatcagca cgggtcgcaa cctgctacgg
    18721 ccagcttcct gtatggcctc gtagaggcta tagccacgct tacgcaggag gtttgtgtag
    18781 tcaaccagca caataccatt cttaaccaca atccctacca gcataatcat gccaacaaaa
    18841 gtaacaatac taagggttaa gcctgtaagc ttgaaagccc acacaacacc aacaaaggta
    18901 acaggaatgg cgaacataat gatgaatgga tccttgaagc tttcaaattg ggcggccatt
    18961 accatgaaaa ccagcaggat gccaaggatg agtatcaggt aaaggtcggc aaacgactcg
    19021 ccctggtcgg aggtttgtcc ggctatcttt acatccacat cattcgggaa tgaggttgat
    19081 gaaacaatac gctgtgcctc catctgccca tctccaagcg aaaccccatt tagctcggcc
    19141 atcaccttaa ccagccgctg ctgctcctta cggttaatct gcagcggact tgttccggtt
    19201 tcaatctggg caacagcata aagcggaatg tcctgattaa gcaggttttt cagctggata
    19261 ttcctgattt ggttgatgtc gttccggtag ctgggcgagt agcgaagcgt aatgtcgaac
    19321 ttatcgccat cctccttata ttcacctgct tcggttccgt aaatgctttg acgtacctga
    19381 agcccaatca tagctgcgtt aagccccatg gccgaggcct tctgtttgtc tattttaact
    19441 tgaacttcga gtttcccctt atccacatcg ttgtccacat tggtaaagta gtccgattgc
    19501 ttcagcttgg ctgttaacgc ctcggcagtt tggttgatgg ttgaaaagtt gttacccgag
    19561 atttcgattt cgatgggctt tttgttgcct gtcaaggctg tggttagcac acttccgccc
    19621 aggagggtaa aattctcaat ttcaggaatt tccgaaattc ttggacgcag acggttggca
    19681 atttcgcgag ctgatgcgct cctttcgttc ggtttacgca ggtgacacat gatagtggct
    19741 acgtttttcc cctcgctaaa tcctactgac gagagaattc catcctcggt ttggccggca
    19801 atagaaaaaa ttgagcctgg agccatctcg ggaatttcct cctcaaagat ttttaaaacc
    19861 acgttggcta cactatccgt ttttgctgca gcggttccta cctcagtttc aaccacggcc
    19921 actacgtcgc cggcatcaaa ttcgggaata tagtcggttc ccatatttct gcccatccaa
    19981 acggaaagac caagaatgat ggtaacaatg ataacggtaa tggccttgtg ataaactacc
    20041 cacgctaaag cctttttgta ccagccttcc agcccggtta gaagtttctc gctggcagta
    20101 aagaacctgt tgtgcctatg tacctctccc ggtttaatgc cttttaccaa gagcgatgag
    20161 gccatagggg tgagcgtaag agccgtgaag agggaagcca aaagggttac cgaggtgaga
    20221 atggccagct gcttgaacat gattcctaca attcctccca tgaaaaccat cggcagaaaa
    20281 acaacaatgg tggtaaaagt tgaggctgat atggccaaac ccatttcgct ggtaccaaat
    20341 atggccgcct gtttaggctt ttcgccgttt tcaacatgtc tggtaatgtt ttcaagcact
    20401 acaatggcat cgtcaaccac cattcctgaa acaactataa gcgacatcag gctaaaaatg
    20461 ttgattgtcc atcccgcggc aaacattacg atgaaggcta caattagcga aaagggaatg
    20521 gtaataaaaa tgatgaagct ctggttccag tttcgaagga acgccagcac aaccaaaatg
    20581 acgaagaaaa gggcgtacca aagcgtttcg gataggttgc taatagactc cgagaccagc
    20641 tcatcgctct tcatcacctc gctcatggta acatctgcag gcagtgtctt ttttagctca
    20701 tctaccttaa gcctgatagc gttggccaca ttaagcgtat tttcgccact ctgcttttga
    20761 atcatcataa taagcccttt accgctggag gttcgagggt actcgtcctt ctccttaaac
    20821 tcatcgtgga tgtctgccac gtcgcttagc ttgataacct taccgttgaa gctggtcagg
    20881 ggcatttccg ccaactcgtc aatgctggca acatcgcccg gaatctgaac ggcataatcg
    20941 ctgctgccaa tcttgatgtt tcctccgggt attgagagat tggaagcctg aaggatgttg
    21001 gcgatttgcg acatggagag gttataggcg ttgagtttaa gggggtttac gttgatcttg
    21061 atttcgcgtt caggttcccc aatgataatt actgatccta cacccgaaac tttgcgcagc
    21121 ttgctggcta tttcgtcgtt taccaacttg ttgagtgcac catagctttc ctttgcccca
    21181 atgctgtaag ccaatacagg cattagcgag ctgtttacct tgaaaatagt tggattctgg
    21241 gcttcgcggg gcaacttgtt tttaaccagc tctatcaggt cgcgaacatt attggctgcc
    21301 gaggtaatat cggcacccca gtcgaattgg agctggacaa acgaaacgtt ctccttgctt
    21361 tgcgaggaga tgtttttcag gttctctgtg cccgaaagta tcatctccag ctctttggta
    21421 atctgctgct caacttcgct agcgctagcg cccgggtaaa ctgtgattac tgttagggtg
    21481 ggaagttcca tatcgggcat gatgtcgagc ggtagccggg tgagggatac cactccaaac
    21541 agcaaaacag ctataaaaag cattgtaatg gctactggcc ttttgaccgc tacttctggc
    21601 aatttcatcc gtgcaaaatt ttaatattgt gagttggcaa taaactacaa tttggcattt
    21661 tatactcgtt aacgttcgcg tagaacatcc attgctttga aatcctttgt gctacatggg
    21721 tgtttcctgt tttcatcaaa gtattttttt tcagcacgca tctaaaaccc taaacgagcc
    21781 tattttccgc tgacccgtac acgttgtccc tgacttaccc tggttttccc atcaagaacc
    21841 acttcaacgc cgggttcaat ccctttcacc gcaacatatt cgcctattga ttcaagcctg
    21901 gatatcggta ttcggttaat ggtgtcgttg gttaccgaga aaaggtaatc atccgaagtg
    21961 ccgggtaggc ggtaaatgct ttttaatggc actacagaag ccttaatttc gggcagcttc
    22021 acggtgatgt aggcaaacat tccgggttta atctccagct gcgggttact aatggtaacc
    22081 tccactgcag ctgagtgggt ggtggttgac agaatttgtt ttatggttcc cacctttccc
    22141 tggtaggttt tgcctgcaac gccgtcgaag gtaacctcaa cctcaagccc ttttctgatt
    22201 acgcctaggt ccttttcgtt gacatcgaac ctaactttaa cggggttaag ctgcatgagc
    22261 ctaacaattc ccgagcgcat ggagtagccc ggctctagcc cagggtttag gaagtaaacc
    22321 tcaccctcct ccatcatgcg ctcaacaacc ataccgctga agggggcata aacccttgtg
    22381 ttttttttga ccatttgtac tttggcctgt gaggcatcat actgagcctt aacgtggtca
    22441 tactccattt cgctgatgct tcctttttcc ctgaggcgcg aaactcgatc gaaatccttt
    22501 tttatggttt cgttttcaat gattgtctgg gtgagcagtt cgtctgatag ctcaactatc
    22561 aggtcgcctt cgtgcacgtt ttgcccttcg tcgaagtaaa ttttttcaac ctttccggga
    22621 agagttgccc ccaggttggc ctctcgattg gcaaaagctg ttccggtaaa ggaaagggtg
    22681 gggctataaa ttcgctcctc aaccacaaca gtcgaaacca acggaacgtt ttcctccgtt
    22741 gcgggaactt ctgttttact ggtcttgcac cctgttagca ctagtgcaat agcaagtggg
    22801 gttagcccta ggatagtcgg tttcatattt gccattatta aaatattgtt attctgttaa
    22861 ttccttttgt ttgcagctaa agggtaccgg taaccttttt aaggttggta atgttaagcc
    22921 ggtactccat tcgggcatct ataagatcgg agtaggcatt ttgccaaagg gtttgtgcct
    22981 caagaacatc ggaggtttta agtttgcctt catcaaaagc attacgggta acctttaggt
    23041 tttcctccgc ctgttttagg ttttcctcgg ccatttgaac c
//
LOCUS       DAOPHX010000025        22998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_2336_length_22998_cov_6.239289, whole genome shotgun sequence.
ACCESSION   DAOPHX010000025 DAOPHX010000000
VERSION     DAOPHX010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 22998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22998
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2336_length_22998_cov_6.239289"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="PK502_03240"
     CDS             <1..1075
                     /locus_tag="PK502_03240"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPG99862.1"
                     /translation="IINREGGTFKLAPWDWWHYAEKLKKEKFNLDESELKPYFKLENV
                     RDGMFYVANQLYGITFTRRTDLPIYHPDVEVFEVKNETGAHIGLLYLDYFPRAGKRVG
                     AWCGRFRQQTYENGKRITPIVTMVTNFTPPTGNTPALLTWDEVTTLFHEFGHALHGLF
                     TDGPYDRIAGSLPRDMVELPSQVMENWAGEPRVIKVYAKHYQNGSPMPEDLINRLKKS
                     MTFNQGFETVEYIAASVLDLDWHSITEPQKVDVLQFENNSMKRIHLLDEIYPRYRTTY
                     FNHIIGGYAAGYYVYLWAAVLDTDAFNAFAETGDIFNHEVATRFRKHVLTEGGNDEGM
                     IQYVKFRGKEPSVEPLLKKRGFK"
     gene            1380..2738
                     /locus_tag="PK502_03245"
     CDS             1380..2738
                     /locus_tag="PK502_03245"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPG99863.1"
                     /translation="MKRINIYSALTVSLLVVSFGCHKPKPYDLIVENAIIFETTEGKA
                     SPALKTILINADTIAGIVDYETPVRTKKVVDADGRLVTPGNIDTHVCLQNVIEGSKNP
                     QSSYPKVLTNYYRNNFSRRYLPYGITTALDLEPNDSWTSQILDWTPNPKFTTLLVAYQ
                     LPKVSFISSNRPTTPNIDSIAKALQSKGLGYVFLDEQEAVFPQKVYEMLKGKGMVVFT
                     RPHFGTFLRDSSKNIVSISTLISNALHYISKQNDLSRQIKNVYGSIDTFPKELFILEA
                     FKYMVENEPALLDKIAQEFGNRKATISSDLYGMAEFAGLTPERNLSLTLPRELQHRAE
                     YNFNHLLSYVKKLHDMGVKLLIGTNSPLGGLAYETEQMLLSEAGIPASDVLVISTLNA
                     AEALGISSKTGCIKVGSKADLIIYDMNPLEEPSNFTCTRRVINGGKLYKRNSQKTEES
                     EL"
     gene            complement(2769..4112)
                     /locus_tag="PK502_03250"
     CDS             complement(2769..4112)
                     /locus_tag="PK502_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPG99864.1"
                     /translation="MGAFHAYDIRGIYNKDFNKDDAYKIGFFLVELLKTDRVLVGRDV
                     RMSSDEIFTYLTRGITDAGADVYNLGLATTPMVYYATAKGDFTASVQITASHNPKEYN
                     GLKVSGRNAEPIGYDTGLKFIEERMKSSSVVPVKDTGRVIDYDIRKDYITFLNSFKQD
                     YSNLKFAVDCSNGMAALLVKDILGNSPVYIYDELDGSFPNHEANPLIPENVVDLQNLV
                     RKQKCDLGIIFDGDADRVMFVDEHSHFISPDLMIAVLGHYFLEEKGFRGNVLQDIRTS
                     KAVGEYLAPMGATMHTWKVGRAFAAKKLRELDGIFGGELAGHYYFRDFFYSDSGMLAC
                     ILILNVVSKMKRKGISLSQLIGSIATYANSGEINFRIEKKQEAMDAVRNFFTATEKPV
                     AIYDFDGYRVEFSDWWFNIRPSNTEPYLRFIAEAKSTEMLDAKVSQVRRIIEEMK"
     gene            4254..4955
                     /locus_tag="PK502_03255"
     CDS             4254..4955
                     /locus_tag="PK502_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99865.1"
                     /translation="MRFRSKLIWVTLFAIAMGLLESAVVIYMRELLYPGGFSFPLSPI
                     PERLAVTELLRELATLVMLVGVGVVAGRNFPERFAWFIFSFGVWDIFYYVFLKLLIGW
                     PTSMLTWDLLFLIPVTWTGPVVTPVVCSMLMITLALVILRRTGKNPTFRISPLSWVIL
                     IAGAMVVFLSFITDYTRFILHSFTLRELWSLPSKQALFDLSVQYIPFSFPWMIFAAGS
                     AVIIAAGIRIFNENR"
     assembly_gap    5061..5160
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5261..7351
                     /locus_tag="PK502_03260"
     CDS             5261..7351
                     /locus_tag="PK502_03260"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPG99866.1"
                     /translation="MIAIGAIVSCKSEKQTDNPFLEDYTTPFGVPPFDKIRVEHFEPA
                     IMEGIKQQLAEVEAITTNSEKPTFENTIIPLDYSGELLTKVVLTMENINSANTSEELQ
                     EMLKKVSPVLSKHRDDISMNPKLFERVKMVYSSMNESSYTPEQKKLIENTYKDFVRGG
                     ANLDAAKQEELRKINQELAMLALKFNENLLADNNAYKIVIENKDDLAGLPESVIAMGA
                     ADAKARGLEGKWVYTVDKPSMLPFLTYSQNRKLRDTLYYGYYMRGNNNNANDNKDIIK
                     KIVDLRQQKASLLGFPTHASYVLDVNMAKTPENVYDLLGKLWTPAIKKAKVERDDMQK
                     LANAEGANFKIGAADWWYYAEKVRKEKYDLDEEMLRPYLSLEDVRNGVFNLAGKLYGL
                     KFIKHTDIPVYHPDCEVYEVQEADGRHLGILYLDFFPRDSKSGGAWCTEYRSYKVKPD
                     GTISTPVVSIVTNFTKPAGDTPALLSFDEAETFFHEFGHALHSLFSKVQYPGLANVPQ
                     DFVELPSQIMEHWASHPKYLKEYALHYKTREPMPDKLISKIEASGQFNQGFATVEYLA
                     ASILDMDYHTLTAEKAKIEDVLAFEKASMDRIGLIPEILPRYRSTYFGHIFSDVLGYS
                     SGYYSYIWAEVLDSDAFEAFVESGDIFNKEVAGKFRTCVLEKGGSKEALELYKDFRGR
                     EASIEPLLKNRGLK"
     gene            7654..8409
                     /gene="pssA"
                     /locus_tag="PK502_03265"
     CDS             7654..8409
                     /gene="pssA"
                     /locus_tag="PK502_03265"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HPG99867.1"
                     /translation="MPTGKSITKQLPNVITLLNLVSGCLSIVAALDCDLQLAGLFIIL
                     AAVFDFLDGFTARLIGAYTMLGKELDSLSDVVSFGVAPSMIIYELMRQSLELPKNEGL
                     LNGHWLLLIPFVMAAFSSLRLGMFNLDERQSSSFIGLPTPANALMIVGLTFGLNSTWA
                     DVYGIFTSSVWALTAIVALQSFLLVCELPMFSFKIKSLKLSVAYKQLLFLSGAAVLVV
                     IFLKAALSLIIVWYVFLSLVFYLTEKMGKKQHV"
     gene            8513..8770
                     /gene="purS"
                     /locus_tag="PK502_03270"
     CDS             8513..8770
                     /gene="purS"
                     /locus_tag="PK502_03270"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HPG99868.1"
                     /translation="MKFTAEINVMPLKALLDPQGKAVNSTLHGIGHTEVSNVRIGKHI
                     TMEVESASREEAIEKVTEICSKVLSNPVMEGYDFTLNEIKS"
     gene            complement(8772..9884)
                     /locus_tag="PK502_03275"
     CDS             complement(8772..9884)
                     /locus_tag="PK502_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HPG99869.1"
                     /translation="MDTPLNITPISSWFEGLSLPLIIAGPCSAESEEQVMDAALQLAG
                     IERVKVFRAGIWKPRTRPGSFEGAGEKGLRWLQRVQRETGLLVTTEVATPAHVQMALD
                     YGIDIVWIGARTTASPFTTEQLAVALSHSNIPVLVKNPITPDLELWIGALERFNRMGI
                     TKLAAVHRGFYPYEHSRLRNIPKWELAIDLKSRFPDLPILCDPSHIAGRADMVAQVAQ
                     HGLNLSLDGLMVEVHPNPSAALSDARQQVSPHFFSQLLQKLIFRDSSSACSEYVDFIE
                     QTRNKIDSIDQQIIELLANRMKLVEQIGEYKRDNNVTVFQLRRWERILESRVEYGERL
                     GLSEEYIKNLLRMVHKESIRRQAEILGSKSQPNSNE"
     gene            complement(9960..10973)
                     /locus_tag="PK502_03280"
     CDS             complement(9960..10973)
                     /locus_tag="PK502_03280"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPG99870.1"
                     /translation="MPSIYRLLKLNRLVRSNRLKLLLIFMAQLLNRRYYSVRIDPVFA
                     CNLRCRMCYFSRSRKPNPEKFSPDELATIAGKLFGNALQVVVGCGAEPTMYAHFVDIV
                     KKASEYSVPHISLVTNGQLLTRDHLNELAESGLNELVVSVHGASQQTYEHLMVGASWE
                     KLHKLLNSVNEMRAVNRYPNFTLRVNYTVNPLNLHELETFFDAFGQYQINTLQIRPVM
                     NIGGEYEDGFSQSDIHTYSKIIAKVRNYCRERGITLLANTDDPTYQRSSKVSWVLNET
                     YKYVSPAMVVSSDFKWKEETLSAYLRRTGWYATMLKRIFWFQRAEKPDDLAQKYSARY
                     DLF"
     gene            complement(11322..12473)
                     /locus_tag="PK502_03285"
     CDS             complement(11322..12473)
                     /locus_tag="PK502_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HPG99871.1"
                     /translation="MADTTYIFLQQYWWFIVSLLGALLVFLLFVQGGQTLIYTLGKNN
                     TERTMLVNVLGRKWEFTFTTLVTFGGGFFASFPLFYSTSFGGAYWVWMAILFCFTLQA
                     ISYEFRTKPNNFLGQKTYEVFLFLNGLLGTILLGTAVATFFTGSQFSVNKLNLVSSGN
                     PVISSWETPWHGLEAALNFHNLSLGLAVFFLARTLAILYFMNSVNHDVILERSRKHLL
                     YNAIPFLVFFLTFVIWLMVRDGFAVNPSNGAVFMEKYKYLHNLLQMPAVLVLFLVGVV
                     LVLWGIGKSLLSKYSKGIWFAGFGTVFTVLALFLIAGYNNTAFYPSTFNIQHSLTIYN
                     SSSSKFTLTVMSIVSILIPFVVAYIWYAWKSLNVKKIDEDEIKTESHLY"
     gene            complement(12476..14038)
                     /locus_tag="PK502_03290"
     CDS             complement(12476..14038)
                     /locus_tag="PK502_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HPG99872.1"
                     /translation="MEQFDLSLVNWSRAQFALTAMYHWLFVPLTLGLSFIIAFMETLY
                     YKTGDEEWKRITKFWMTLFGINFAIGVATGLILEFEFGTNWSNYSWFVGDIFGAPLAI
                     EGIMAFFMEATFIAVMFFGWNKVSKKFHLVSTWLVALGSNLSALWILVANAWMQYPVG
                     MHFNPDTARNEMHNFWEVLLSPVAVNKFLHSITSGYVLSAIFVIGVCAWFMLRGRHQL
                     MVKRSVTIASVFGLVTSLFLLVTGDGSAYQVAQKQPMKLAVMEGLYEGKEGAPLTAIG
                     ILTPGKQYDDSKDPYIFKIQIPRLLSWLGYRSGDAFVPGVKDVIEGGYVYRDGNGNER
                     VAPSVEQKMAKGKIAIGALAEYKKAAEAGDSSAMAYHKKLLDENFEYFGYGYLNDPKS
                     VIPNIPLTFYSFHVMVALGFLFIIFFIFMLYLVLFRQFKVKRWLLYLSIVMIPLAFLA
                     GQAGWIVTEVGRQPWVIQDVLPTVAAISRIDTSAVQITFFLFLVIFTALLIAELRIMF
                     KQIKIGPKEGGK"
     gene            complement(14045..14260)
                     /locus_tag="PK502_03295"
     CDS             complement(14045..14260)
                     /locus_tag="PK502_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HPG99873.1"
                     /translation="MELPGKIFRFYYEGFRSMTVGKTLWVIILIKLFVMFAILKIFFF
                     PDILKKNFKTDTERSKYVIEQLTSNQK"
     gene            complement(14353..15336)
                     /locus_tag="PK502_03300"
     CDS             complement(14353..15336)
                     /locus_tag="PK502_03300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HPG99874.1"
                     /translation="MEFRTEVAVDDFPFRLGYATPTLFCGSCFTENIGGIMRTLKMPV
                     LVNPFGVVYNPLSVAQVLQRSVAGKIYNADELQFRDGLWFSFDFHSRFASPNRNACIA
                     LLNEAVSSAKDFISTTQCIVVTFGTARVYKLKGSGEVVSNCHKVPAVEFENKLLAVDE
                     VVEIWSNLISMLLGLNPSLKLVLTVSPIRHWKDGAPGNQLSKSTLIVAVHRLVEMYSD
                     NVFYFPSYELMMDDLRDYRFYDDDMLHPSPKAIEYIWSKFSRVLIDDDSMKLAAQIQK
                     IIQAAGHRPFNTDTPEHKRFVDKILKSIQDIKLIHPSINFEREIAILKGEQ"
     gene            complement(15326..16192)
                     /locus_tag="PK502_03305"
     CDS             complement(15326..16192)
                     /locus_tag="PK502_03305"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HPG99875.1"
                     /translation="MKNSTALINVESVHQEWLKTNGYRLFTWYIQPSSPKAAVLLVHG
                     LGEHSMRYQTWAKQFADAGFAFYCWDHVGHGQSSGQRGCVPNYEHLLQEVDFIINKFN
                     RQIGSIPLVLYGHSMGGNIALNYAIRRPSKINLLVVTSPWLKLVSPINPALKSLVKLL
                     DLCLPFLPMNSGIRPEQVSHEMKVVQEYATDPLIHRRITPRLYLAMERAAEYAMNHSE
                     QITIPTLLMHGSSDTLLSSMATSEIAAKMHHSTYVEWDELYHELHNETKSNEVFKTIV
                     NWLNVKIAQYGV"
     gene            complement(16543..17793)
                     /locus_tag="PK502_03310"
     CDS             complement(16543..17793)
                     /locus_tag="PK502_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG99876.1"
                     /translation="MERKSSKLEELAMSGSPIQLAIRRLKASWIAVASFCLIGIVVLI
                     SILGYLITPDPTPYANNQHLELSSCKPGAEVTMLKVRRQQDIQHRSWFGRMLYGDESD
                     YREIPINSFYFEDASIVIEGYNPIPESEPVVARFSLAEVAYRIEPPAKPIIRNDSVLF
                     VQADGGATAMTVSQLQERVEKENFVTHTYILGTDRFGRDLLSRMIIGARVSLSVGFIA
                     VAISLLIGILLGSLAGFLRGWVDSAIMWFVNVVWSVPTLLMVIALTMVIGKGFWQIFV
                     AVGLSMWVDMARVVRGQVLSVRENGYVEAATVLGLSRFRVIFRHILPNIMGPVAIVCA
                     SNFATAILLEAGLSFLGIGVQPPMPSWGSMIRDHYGYIIMDKAYLAILPGFAIMMLVL
                     AFNLLGNSLRDALDPQTIRNNSPK"
     gene            complement(17760..18878)
                     /locus_tag="PK502_03315"
     CDS             complement(17760..18878)
                     /locus_tag="PK502_03315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG99877.1"
                     /translation="MLTIATIAITLLYVVLIVLLIVGLVQPSAGHNQAFGQLAQPVSV
                     VVAFRNEAPNLTRLLEALSRQTLDASKWELIMVDDGSTDGGDKIAQSFHNLPGLRLVK
                     LQKGITGKKQALLFGVELARFNLVAITDADCVPAATWLNDISRRANGYALLQGCVLVE
                     RNSNLLSWFEALDYASLMAVSAGSFGLKRPVVAASANLVFRKDMINVRPETLRVDVAS
                     GDDMFLLHEVKKDHSNNMLFSTNLAGSVLTRFDGGFVGAIRRRRRWASKAPAYTDIET
                     ILVGLIVLVFNLWLLVLPMICIFNLEAWNLLVGSLLVASLANFCLLFIYLKQTNQLKL
                     ILVFLPLQLIYPIYLCTVAIAGFAGGQNWKGRAVSWKN"
     gene            complement(19115..20008)
                     /locus_tag="PK502_03320"
     CDS             complement(19115..20008)
                     /locus_tag="PK502_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPG99878.1"
                     /translation="MVDKSYIHSLITLLDDPDPEIFNAVSEKIITQGITVVPLLEGAW
                     GSSDSRIFQERLENLIQTIQFNTVANALTQWKDSESNNLLEGAVLVARLQFPDLSLQP
                     IELEIKKLQKDIWLEINSNLTALEKVRVINHVIFTIHKYEPNTSNFFLPGNHLINHVV
                     ETKRGGPIMLAILYAEIAQRLGLPISCVNLPKNFVLAYQDRYRVPAERKGKDPESILF
                     YINPFNNGSVFSRNEIELFLSRQQLEPKPEYFLPCANTTTISQLVFNLIYSLERNGNR
                     SKANEYKKLLQILEKDGEEQG"
     gene            complement(20019..20894)
                     /locus_tag="PK502_03325"
     CDS             complement(20019..20894)
                     /locus_tag="PK502_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HPG99879.1"
                     /translation="MRVIESSELILNQDGSVFHLHLKPEQLADTVILVGDPGRVETVS
                     SYFDRVELTVSNREFKTCTGTYKGKRISVVSTGIGTDNIDIVVNELDALANIDLASRT
                     VKANHTKFTLVRLGTSGALQPEIELGSSVLTEISIGFDGLLNFYANRNGICDLEMEQA
                     FLKHTQWNPLCAKPYFCHSSRKLVELFAGITVKGVTISAPGFYGPQGRVLRLPLADPT
                     LNDKIVDFRYKGYKITNFEMESSAINGLSLLLGHDSVTICTIIANRVVKDAKVDYQLA
                     MKNLIENTLNRLASI"
     gene            22052..22714
                     /locus_tag="PK502_03330"
     CDS             22052..22714
                     /locus_tag="PK502_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99880.1"
                     /translation="MNSITKRCHIALLLILTASPAFCQSGDTVEAKQGVKVELGYMVI
                     SNSLNADDYHDFKYMHVIDNANAFIRGANLKFTFPTRWKYLDATAGALLIRCLDESGT
                     SAIDVSHINADAYNLNGGGVYLGISPKLKGKHFGLTSDLAVGVLSFKEYVSIVINDRE
                     PNVDEHNLKASYGLGAVSSVGFYVSFWRMGINPSLTCIFSGGASSSFTFWGLNIPITI
                     QF"
BASE COUNT         6396 a         5407 c         5180 g         5915 t
ORIGIN      
        1 catcatcaac cgggagggcg gtacatttaa actggctcct tgggactggt ggcactatgc
       61 cgaaaagctg aaaaaagaaa agttcaacct tgacgaatcg gagctaaagc cctactttaa
      121 gcttgaaaac gtgcgcgacg gcatgttcta cgtggccaat cagctctatg gaataacatt
      181 tacccgccga acagaccttc ccatctacca tcccgacgtg gaggtttttg aagtaaagaa
      241 cgaaaccggg gcacatattg ggctgctcta cctcgactac ttcccgcgag ccggcaaaag
      301 agtaggggca tggtgtggcc gtttccgaca gcaaacctac gaaaacggaa aacgaattac
      361 cccaattgta accatggtta ctaattttac ccctcccact ggaaacaccc cagccctcct
      421 cacatgggat gaggtaacta ccctgttcca cgagtttggc catgcccttc acggcttatt
      481 taccgatggc ccatacgacc gcattgccgg aagcctaccc cgcgacatgg tagagctgcc
      541 ctcacaagtg atggagaact gggccggaga gccccgcgtt attaaggttt acgccaagca
      601 ttaccaaaat ggcagcccta tgcccgaaga cctcatcaac aggttgaaaa agagcatgac
      661 ctttaaccag ggttttgaaa ccgtggagta tattgctgca tcggtgcttg acttggattg
      721 gcacagcata accgaacctc agaaagttga tgtgctgcaa tttgaaaaca actccatgaa
      781 gcgaattcat ctgctagatg agatataccc acgctaccgc accacctact tcaaccacat
      841 cataggtggg tatgcagccg gctactacgt ttacctttgg gcggctgttc ttgataccga
      901 cgcatttaac gcctttgccg aaaccggtga tatttttaac catgaagtgg ccactagatt
      961 ccgcaaacac gtattaactg aagggggaaa cgatgaggga atgattcagt atgttaagtt
     1021 tagagggaaa gaaccctcgg ttgaacccct tcttaaaaaa agaggattta aatagtcttt
     1081 cggagtgctt ttacatattt aagggtttgt ttgggtcaaa tgggctgtag ctaaatgctg
     1141 cagcccattt ttttttcaaa tccagctcag ctgcccacca tgcacttttg gtcaaaaatt
     1201 ccattcacag cccgacaatt gccgatttag ataagaaggt aacggtaaca agcttataaa
     1261 cagatattac caacccatgg aaaacctcca cccatcatca gactctaccc aaacttatcg
     1321 gtaagactac ccaaaaaatc ctaaaaagta gtacctttga attttcaaca ccaacccgaa
     1381 tgaaaaggat aaacatatac tccgcgttaa cggtgtcatt gctggtagtt tcctttggct
     1441 gccataagcc aaaaccctac gacctgattg ttgaaaatgc cataattttt gaaactaccg
     1501 agggcaaagc atctccagca cttaaaacca tcctgataaa tgccgacaca attgccggaa
     1561 tagttgatta cgaaacacct gtaagaacca aaaaggtagt ggatgccgac ggtcggcttg
     1621 taacacccgg taatattgat acccatgttt gccttcaaaa tgtgattgag ggtagcaaaa
     1681 acccgcaaag cagctatccg aaagttttaa ccaactacta ccgcaacaac ttctccagga
     1741 ggtatttgcc ctacggaatt accacagctt tagacttaga gcccaatgac agctggacca
     1801 gccaaatact ggattggaca cccaatccta aatttacaac cctgcttgta gcctaccagc
     1861 ttccaaaagt ttcatttata agctccaata gacctaccac tcccaatata gacagcattg
     1921 ctaaggctct gcaatcaaag gggctaggat atgtttttct ggatgaacag gaagctgttt
     1981 ttccacaaaa agtttacgaa atgctgaaag gaaaaggaat ggttgttttt actcgtcccc
     2041 attttggaac ttttttaagg gactccagca aaaatatagt cagcatttca acactaataa
     2101 gcaacgcact gcactacatt tctaaacaaa acgatctttc gcgtcaaatc aaaaatgtct
     2161 atggcagcat cgataccttc ccaaaggagt tgttcattct ggaagcattc aagtatatgg
     2221 tagagaatga gcccgcgctg cttgataaga ttgcccaaga gtttggtaac aggaaggcta
     2281 ccatatcatc tgatttgtat ggaatggccg aatttgccgg actcacaccc gaaaggaatt
     2341 tatccttaac ccttccacgc gagctgcagc acagagcgga gtacaacttc aaccatctgc
     2401 tcagctatgt aaaaaaactt cacgatatgg gcgtaaaact cctcatcgga actaatagcc
     2461 cgttaggagg gctggcctat gaaacagaac agatgctgct gtctgaggct ggaatccctg
     2521 cctcagatgt actagtaata tcaaccctta acgctgctga agcattagga attagcagta
     2581 aaactggttg tataaaggtg ggaagtaagg ccgacttaat tatttacgat atgaaccccc
     2641 tggaagaacc ttcaaacttt acctgtacta gaagggtaat taatggtggc aagctataca
     2701 agcgaaacag ccaaaaaact gaagaatcgg aattgtaaaa tcctgatccc tcttcaaaat
     2761 tcaaagggct acttcatctc ctctataatt cttctcacct ggcttacctt ggcatcaagc
     2821 atttcggtgc ttttagcctc ggcaatgaaa cgtaggtagg gctcggtatt tgaagggcga
     2881 atgttaaacc accagtcgct gaactccacc ctgtacccat cgaaatcgta aatggcaacg
     2941 ggcttttcgg tagcagtaaa aaagttgcgc acggcatcca tcgcctcctg cttcttttca
     3001 atcctgaagt taatttcgcc agagttggcg taggtggcaa tgctaccaat tagttgggaa
     3061 aggctgatcc ctttcctttt catcttactt acaacgttta aaatgaggat acaagccagc
     3121 attccagagt cggagtaaaa gaagtcgcgg aagtagtaat gtccggcaag ctcgccgcca
     3181 aaaatcccgt caagctcgcg tagcttcttt gcagcaaaag ctcttcccac cttccatgtg
     3241 tgcatggtag cacccattgg ggcgagatac tcgccaaccg cttttgatgt cctgatatcc
     3301 tgcaggacgt tcccccgaaa tcctttttcc tccagaaagt agtggccaag cacggcaatc
     3361 atcaggtcgg gtgaaataaa gtgcgagtgc tcatcaacaa acatcacccg atcggcatcg
     3421 ccatcgaaaa taatcccaag gtcgcatttc tgctttctca ccaggttttg caggtcaacc
     3481 acattctcgg gtataagggg gttagcctcg tggttgggga atgaaccgtc cagctcatcg
     3541 tagatgtaca ccgggctatt gcccagtatg tcctttacca aaagtgcagc cattccgttg
     3601 gagcagtcaa ccgcaaactt caggtttgaa tagtcttgct tgaagctgtt gaggaaggtg
     3661 atgtaatcct tccttatatc ataatctatt acccttccgg tgtccttcac gggtaccaca
     3721 ctggagcttt tcatcctctc ctcaatgaat tttaaaccgg tgtcgtagcc aataggctcg
     3781 gcattcctgc ccgatacctt caacccgttg tactccttgg ggttatgcga tgcggttatc
     3841 tgtaccgatg ccgtgaaatc tcccttggcg gttgcgtagt aaaccattgg cgtggtggct
     3901 aatcccaggt tataaacatc cgcaccagca tcggttatac cacgagtcag gtaggtgaaa
     3961 atttcatcgg acgacatgcg aacatcacgt cctaccagca ccctgtcggt tttcaaaagt
     4021 tcaacgagga aaaagccaat tttataggca tcatccttgt taaaatcctt gttataaatt
     4081 ccacgaatat cgtaggcatg aaaagcaccc atagtcctgt ttaccggtta agttatcatc
     4141 aatttacttt ggctggcaaa attaaaaaca ttattccttg ttgaaacctt ttggccataa
     4201 tattgaccaa agtcagtaaa aaagagtaaa tttgataggt taaacagaaa attatgcgat
     4261 tccgaagtaa attgatatgg gttacgcttt ttgccatagc catggggcta ctggagagcg
     4321 ccgttgtaat ttacatgcgc gagctcctat accctggtgg tttttccttt cctctctccc
     4381 caattcccga gaggttggca gttaccgagc tgcttcggga gttggcaacc ctggttatgc
     4441 ttgttggagt tggcgtagtg gccgggagga acttccctga gcgttttgca tggtttattt
     4501 tcagctttgg cgtttgggat atattttact acgttttctt aaagctgctt ataggctggc
     4561 ccacctcgat gctaacatgg gatttgctat tcctcattcc ggttacgtgg actggccccg
     4621 ttgttacgcc cgtagtatgc tccatgctga tgataaccct tgcattggtc atccttaggc
     4681 gaacggggaa aaacccaaca ttcagaattt caccactctc atgggtaatt ctaattgccg
     4741 gagccatggt tgtattccta tcatttatta ccgattacac taggtttatt ctgcatagct
     4801 tcactttacg ggagctatgg tcgttgccat caaaacaagc tctgttcgat ttgagcgtac
     4861 agtacattcc cttttcattc ccatggatga tatttgctgc aggcagtgct gtaataatag
     4921 ccgcaggtat tagaattttt aacgaaaacc gatagcgtta atagctaaaa aaacgtgaat
     4981 gccgtacagg cttccggcaa aagccgtgca ggtttagttt tccttatcat tcaccatcaa
     5041 tctttcattt ttttaaatat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 tatccattat ctttgcttgc agacaagtaa atagctattc gtgttaactt aaattaaact
     5221 atacaatcaa atgaaacgat acagctggat tttaatgaca atgatagcca taggcgctat
     5281 tgtctcatgt aaaagcgaaa agcaaacaga caacccattc ctggaggatt acaccacgcc
     5341 attcggcgtt ccaccatttg ataagataag ggttgagcac ttcgaaccgg caatcatgga
     5401 gggtattaag caacagctag ctgaagttga agctattact actaacagcg aaaaacccac
     5461 atttgagaat accataattc cgttggacta tagcggtgag ttgctgacaa aggttgtgct
     5521 gacaatggaa aacatcaact cggccaacac cagcgaagag ctgcaggaga tgcttaaaaa
     5581 agtttctccg gttctatcga aacaccgcga cgatatcagc atgaacccta aactttttga
     5641 acgggtgaag atggtttaca gcagtatgaa cgaaagcagc tacacccccg agcaaaaaaa
     5701 acttattgaa aacacctata aggacttcgt aagaggtggc gccaaccttg atgctgccaa
     5761 gcaggaagag ctcagaaaaa taaaccagga gcttgccatg cttgccctaa agttcaatga
     5821 aaaccttctt gccgacaaca acgcatacaa aattgttatt gaaaataagg acgatcttgc
     5881 aggtcttccc gaatcggtta ttgctatggg tgctgccgat gcaaaggcaa gaggtttgga
     5941 ggggaaatgg gtgtacaccg tcgataagcc aagcatgctt ccattcctca cctactccca
     6001 aaatcgcaag ctacgcgaca ccctttacta cggctattac atgcgtggca acaacaacaa
     6061 tgccaacgat aacaaggata taattaaaaa aattgttgac ctaaggcaac aaaaggcctc
     6121 actcttgggc tttccaaccc acgccagcta tgttctcgat gtgaatatgg caaaaacgcc
     6181 cgaaaatgtt tacgatttac taggtaaact ctggactccg gcaataaaaa aagcaaaagt
     6241 tgaacgcgac gacatgcaga agctggccaa cgctgaggga gcaaacttca agattggtgc
     6301 agccgactgg tggtactacg ccgaaaaggt tcgtaaggaa aaatacgacc tcgatgagga
     6361 gatgcttcgc ccctacctgt cgctcgagga cgtgaggaat ggcgttttca acctagcagg
     6421 gaaactttac gggttgaagt tcataaaaca caccgatatc cccgtttacc atcccgactg
     6481 cgaggtttat gaggtgcagg aggccgatgg ccggcattta ggaatcctct acctcgattt
     6541 cttccccagg gatagcaagt cgggtggggc ctggtgcacc gagtaccgtt cctataaggt
     6601 caaacccgat ggcaccattt ctacccccgt ggtgtccatt gtaaccaact ttaccaagcc
     6661 agcaggtgat actcctgccc tgttgagttt cgacgaagcc gaaactttct ttcatgaatt
     6721 tggtcatgcc ctgcacagcc tgttctctaa agtgcaatac cccggactgg ctaatgttcc
     6781 gcaggatttc gttgagctac ctagccaaat catggagcat tgggccagcc atccgaaata
     6841 cctgaaggag tacgcccttc actacaagac aagggaaccc atgcccgata agctgatttc
     6901 aaaaattgaa gccagcggcc aattcaacca gggctttgca acggttgagt atctggctgc
     6961 atccattctt gatatggact accacaccct taccgctgaa aaggcaaaaa ttgaagatgt
     7021 gctggccttt gaaaaagcct cgatggatag aattgggtta atacccgaaa ttctaccccg
     7081 ctaccgcagc acttactttg ggcatatttt cagcgatgtg ctgggctact caagcggata
     7141 ctactcctac atttgggccg aagtgctcga tagcgatgcc tttgaagcct tcgtggagag
     7201 cggcgacata ttcaacaagg aggtagcagg aaaattccgc acctgtgttc tcgaaaaggg
     7261 tggttccaag gaggccctgg agttatacaa agattttcga ggtcgtgaag ccagcattga
     7321 accgcttcta aagaacagag gattgaaata gcacgccatt gcacaaaaca ccaaaagggg
     7381 ctcaaaccga gccccttttt tagttgcaag tcatactaac ggttgctgcc tggtaaagct
     7441 catcaatctc cggtgttagc ttaatcccga catattcaag tcaacgtaat gagcattcat
     7501 cggcatcccg cctaatgaaa aatttctgaa atgttttctg caacagtttt gcctttttac
     7561 catttaacaa acccaagaat tttcattcat ttagaatttt atttttgaac ttgacgaatt
     7621 tgtactctaa ttttagcccc aaattatagc gccatgccaa caggtaaaag tattactaaa
     7681 cagttgccca acgtaatcac cctgcttaac ctggtttcgg gatgcctttc gatagtggca
     7741 gcgcttgatt gtgatctcca gctggcaggg ctgtttatca ttttggcagc ggtgttcgac
     7801 tttctcgatg ggtttacggc caggctgata ggtgcgtaca ccatgctggg gaaggagctg
     7861 gactcgctct cggacgtggt tagctttggc gttgcaccct ctatgatcat ttacgagctg
     7921 atgcggcaaa gcctggagct gccaaaaaac gaaggtctcc tgaatggcca ttggcttctc
     7981 ctaatcccat ttgtaatggc tgccttctcg tcgttacgat tgggaatgtt taaccttgat
     8041 gagcggcagt catcatcatt cattgggtta cccacccctg ccaatgcgct gatgatagta
     8101 ggccttacct ttggcctcaa cagcacatgg gctgatgtgt acggaatttt cacaagttcg
     8161 gtttgggcat taaccgccat tgtagccctc cagtcgtttc tcttggtttg cgagttgccc
     8221 atgttctcgt tcaagataaa atcgcttaag ctgagcgttg cctacaagca gctacttttt
     8281 ctttccggcg ctgccgttct ggttgtgata tttctgaagg ccgcactttc gctcattatc
     8341 gtttggtacg tatttctatc gctggttttc taccttaccg agaaaatggg gaaaaagcaa
     8401 catgtttgag ctaaattcaa ccggtgattc taaaaatccc ctatttttgt catcataaat
     8461 caagtagtcc tgctaaattt tattgtatag ccggctaaat aagttcaaac gcatgaaatt
     8521 tactgccgaa atcaacgtta tgccgctaaa ggcgctactc gacccacagg gaaaagctgt
     8581 taactcaact cttcatggta ttgggcatac cgaggtgtcg aatgttagaa taggtaagca
     8641 tattaccatg gaggtggagt cggctagccg ggaggaggcc attgaaaagg taacggagat
     8701 ttgctcaaag gtgctctcga acccggtgat ggagggttac gatttcaccc taaacgagat
     8761 taagagctag cctactcgtt gctgttgggc tgagattttg aacctaaaat ttcagcttgc
     8821 cttctgatgg actccttatg gaccatgcgg aggaggtttt tgatgtactc ttcgctcaac
     8881 cctaaccttt ccccatactc caccctcgat tcgagtatgc gttcccaccg acgtagctga
     8941 aaaaccgtaa cgttgttatc acgcttgtac tcaccaattt gctccaccag cttcatcctg
     9001 tttgcaagca gctcgataat ctgctgatca atggagtcaa ttttattacg ggtttgctca
     9061 atgaagtcaa catactcaga gcatgccgac gaggaatccc tgaaaatcaa cttttgcagt
     9121 agttgcgaaa aaaaatgggg acttacctgc tgacgggcat cgctaagggc agccgatgga
     9181 ttgggatgaa cctccaccat aagcccatca agcgaaagat tcaggccatg ctgggccacc
     9241 tgtgcaacca tatcggcacg acccgcaata tggctgggat cgcataaaat aggtaaatcg
     9301 gggaatcggc ttttcaaatc tatggccagc tcccatttag gaatgttccg taggcgtgaa
     9361 tgctcgtagg gataaaaacc gcgatgcacg gcagccagct tggtaattcc catgcggttg
     9421 aagcgttcaa gcgcacctat ccacagctcc aggtccggcg taatgggatt tttcaccagc
     9481 accggaatgt tactatggct aagggctaca gcaagctgct cagttgtgaa cgggctggct
     9541 gttgttcggg cgccaatcca aactatgtca attccataat cgagggccat ctgaacatga
     9601 gcaggagtgg ccacctcagt agttaccagt aagcccgttt cgcgttgaac cctctgaagc
     9661 catcgtaaac ccttttcgcc ggcaccctca aagctgccag ggcgtgtgcg aggcttccat
     9721 atacctgccc tgaaaacctt taccctttca attccggcaa gctgaagggc tgcatccata
     9781 acctgctcct cgctttcggc actacagggt ccggcaataa ttaacggcag cgataacccc
     9841 tcaaaccaac tcgaaattgg tgttatgtta agtggtgtat ccaaacttta acccaataaa
     9901 tatttgacaa agttagtgag aaataatcaa ctggtaaggg ttaaactaac aaccagctgc
     9961 taaaacaggt cgtagcgggc gctatacttt tgggccagat catcaggttt ttcagcacgc
    10021 tgaaaccaaa aaattctctt aagcatggtg gcataccatc cggttcttcg caggtaggcc
    10081 gaaagtgtct cttccttcca cttaaaatcg ctactaacca ccattgccgg agaaacgtac
    10141 ttgtaggttt cgttcagaac ccagctgact ttagacgacc gctggtaggt gggatcatcg
    10201 gtgttggcca gtagggtgat gccccgctcc cgacaataat tccggacttt ggcaataatt
    10261 ttgctgtagg tgtgaatgtc gctttgggag aacccatctt catactcgcc tccgatgttc
    10321 atcaccggac gaatctgaag ggtgttaatt tggtattgtc caaaagcatc gaaaaaagtt
    10381 tccagctcat gcaggtttaa cgggttaact gtatagttaa ctcgaagggt aaagttgggg
    10441 tagcgattta ccgctctcat ctcgttaacc gagttcaaaa gcttgtgtag cttttcccag
    10501 ctagctccaa ccataagatg ctcgtaggtt tgctggctag ccccatgaac cgacacaacc
    10561 agctcgttga ggcccgattc ggcaagctcg ttcaaatggt cccgggtaag tagttggccg
    10621 ttagttacca agctgatgtg gggcactgag tactcactgg cctttttaac aatatccaca
    10681 aaatgtgcat acatcgttgg ctcggcacca caacccacca caacctgcag cgcattgcca
    10741 aatagcttcc ctgcaatagt agccagctca tcgggtgaaa atttttcggg attcggtttt
    10801 cgagaccggc taaaatagca catgcggcac ctcaggttgc aggcaaaaac cggatcaata
    10861 cgaaccgagt agtacctacg gttcagcagc tgtgccatga agattagcag aagcttaagg
    10921 cggttacttc taaccagcct gttgagcttc agcaacctgt atatactagg cattatccga
    10981 aggatgtttc ctctctgtac gcctaattgc ccatagggac aacaggtagg ttaggtaaat
    11041 caacaccggc cagcttaaca gccataaaat tccggaaagg tatttcatcg gaaaatattt
    11101 attaggttaa ttgctatagg ttacaatttt atcttttcgt ttcctcctac ccaaaaagct
    11161 catcacccaa catatttcat ccgtagttga atcttcctgg cttataaacc acagctgagt
    11221 gctagagatg ccacctgaat gttttacttg ctttaatatt caaagtaaag atgaacgggt
    11281 agataccata gaaacatcat tgtttaaagt aaagtccaaa gttagtagag atgcgattcg
    11341 gtcttaattt cgtcctcatc aatttttttc acattcaagc ttttccaggc gtaccagatg
    11401 taagccacca caaagggaat aagtatggaa acaatgctca tcaccgtaag ggtgaacttg
    11461 ctcgacgagc tgttgtagat ggtaagagaa tgctggatat tgaaagttga agggtaaaag
    11521 gcggtgttgt tgtagcctgc aatcaggaaa agagccagga cggtgaaaac ggttccaaaa
    11581 ccggcaaacc aaataccttt tgaatatttg gaaagaagcg acttaccaat tccccaaagc
    11641 accagcacca cgccaacaag gaaaagaacc agcacagctg gcatttgcaa caagttatgc
    11701 aggtacttgt atttctccat gaaaacagcg ccgtttgaag gatttacagc aaatccatcc
    11761 cttaccataa gccaaattac aaaggtgagg aagaatacca gaaaagggat ggcgttgtag
    11821 agcaggtgtt ttcgggagcg ttcaaggatg acatcatggt taacgctgtt catgaagtat
    11881 aggattgcca gcgttcgggc cagaaagaaa acagccagcc ccaacgaaag gttatgaaag
    11941 tttagagcag cctcgagtcc atgccatggg gtttcccagg acgagattac ggggttgccc
    12001 gagctgacca ggttgagctt gtttaccgag aattgcgatc cggtaaagaa ggtggccacg
    12061 gctgtgccca aaaggattgt acccagcagc ccgttaagga aaaggaacac ttcgtatgtt
    12121 ttctgcccca gaaagttgtt gggtttggtc cggaattcat aggaaatagc ttgaagggtg
    12181 aagcagaata gaatggccat ccaaacccag taggcacctc caaagctggt agagtagaat
    12241 agcggaaacg atgcaaaaaa acctccacca aaggtaacca gcgtggtgaa agtaaactcc
    12301 cacttgcggc ccagcacatt caccagcatg gtgcgctcgg tattgttctt accaagggta
    12361 taaatcaagg tttgtccacc ctgaacaaat agcaagaata ctagtaaggc acctaaaagc
    12421 gaaacgatga accaccagta ttgctgcagg aatatgtagg ttgtgtcggc catggctatt
    12481 ttcctccctc tttaggtcct attttaattt gcttgaacat aattcgaagt tcggcgatga
    12541 gcagtgcggt aaagattacc aggaacagga aaaaggtaat ctgaacggcg ctggtatcaa
    12601 tccttgagat tgctgcaacc gtggggagca catcctgtat gacccagggt tggcgcccta
    12661 cttcggtaac aatccaaccg gcctgaccgg caagaaaagc tagcggaatc atcacaattg
    12721 agaggtagag aagccaccgc ttaaccttga attgccgaaa tagcaccagg tagagcatga
    12781 aaatgaagaa aataataaaa aggaatccca gcgccaccat aacatggaag ctgtagaagg
    12841 taaggggaat attgggtatt acgcttttgg gatcgttgag gtagccgtag ccgaagtact
    12901 caaaattttc gtcaagcagc ttcttatggt aagccattgc agaggaatct ccggcttctg
    12961 cggccttctt atattctgct aaggcaccta tggcgatttt tcccttggcc attttctgct
    13021 caaccgatgg ggctacccgt tcattcccat taccatccct gtaaacatat ccaccttcaa
    13081 taacgtcctt tactccggga acaaaggcat cgccggaacg gtatcccaac catgaaagaa
    13141 gccttggaat ctgaattttg aagatgtagg ggtcctttga atcgtcatac tgcttgccgg
    13201 gggtaagtat gccaatggca gtgagaggtg caccctcttt gccctcgtag agcccttcca
    13261 tcaccgccag cttcatgggc tgcttctggg caacctggta tgccgatcca tcacctgtta
    13321 ccagcaaaaa cagggatgtt accagtccaa acaccgaagc aatggtaacg cttcgtttaa
    13381 ccataagctg gtgacgacct ctgagcataa accatgcgca tacaccaatc acaaaaattg
    13441 cggatagcac ataccccgag gtgatggagt gcaaaaattt gttaaccgca accggcgaga
    13501 gcagcacctc ccaaaagttg tgcatctcgt tacgggcagt atcggggtta aagtgcatgc
    13561 ccacaggata ctgcatccag gcgttggcca caagaatcca taaggccgat agatttgaac
    13621 ccagcgctac cagccacgta gataccagat ggaacttctt gctaaccttg ttccacccga
    13681 aaaacatcac agcaataaaa gtggcctcca taaaaaaggc cataattccc tcaatagcca
    13741 gcggagcacc aaaaatatcg cctacaaacc acgagtagtt gctccagttg gtaccaaact
    13801 caaattccag aataaggcct gttgccaccc caatggcaaa gttgatgccg aagagggtca
    13861 tccagaactt ggtgatccgt ttccactcct cgtcgccggt tttgtagtat agcgtttcca
    13921 tgaacgctat gataaacgat aagcctaagg taagcggtac aaatagccag tggtacattg
    13981 ccgttagcgc aaactgggct ctggaccagt tgacaagtga taaatcgaat tgctccatag
    14041 tcagctattt ttggttactg gttagttgct ctattacgta cttgctgcgt tcagtatctg
    14101 ttttaaagtt cttctttaag atatcgggaa agaaaaagat cttaagaatg gcaaacatta
    14161 cgaagagttt aatcagaata atgacccaca atgttttccc cacggtcata ctcctgaatc
    14221 cctcgtagta aaatcggaaa attttacccg gtagctccat ggtcaaaata agttatgatt
    14281 tttcaaaatt aacaaaaatt aacacgaatg gttggctgtc ccgattgaat ttaacaaagt
    14341 tatctggtag aactattgct cgcctttaag aatggctatt tccctttcaa aattgatgga
    14401 aggatgaatg agcttgatgt cctggattga ttttaaaatt ttgtcaacaa accttttatg
    14461 ttcgggtgta tcggtgttaa acggccgatg tcccgctgcc tggattattt tttgaatttg
    14521 ggctgccaac ttcatggaat cgtcatcaat tagaaccctt gaaaacttac tccaaatgta
    14581 ctcaatagct tttggtgaag gatgaagcat atcgtcgtcg taaaacctgt agtcgcgcaa
    14641 atcgtccatc atcagctcat agctagggaa gtaaaaaacg ttatcgctgt acatttcaac
    14701 caacctgtgg actgcaacaa ttagggttga tttgctcagc tgattaccgg gtgccccatc
    14761 cttccagtgc ctgattgggc taacagttag cactaacttt aggctggggt ttaaccccaa
    14821 aagcatggaa atcaggttgc tccatatttc cacaacctca tcaacagcca agagcttatt
    14881 ctcaaactca acagctggaa ctttatggca gttggatacc acctcgccac taccctttaa
    14941 cttgtaaacc ctggcggtgc cgaatgtaac aacaatgcac tgggttgtgg aaataaaatc
    15001 tttggctgaa ctgaccgctt cgtttagcag ggcaatacag gcattgcggt taggagaggc
    15061 aaatcgggaa tggaaatcaa aactaaacca caagccatct cggaattgca actcgtcagc
    15121 attgtagatt ttacctgcca ctgagcgttg caaaacctgc gcaacggata agggattgta
    15181 aaccacacca aaggggttaa ccagaacggg cattttcaac gttcgcatga tgccgccaat
    15241 gttttccgta aagcatgaac cgcaaaaaag ggttggtgtg gcatagccca gtctaaaagg
    15301 aaaatcatca accgctacct cagttctaaa ctccatattg ggcaattttt acattcaacc
    15361 aatttacaat cgttttaaaa acttcattgc ttttagtttc attgtgaagc tcatggtata
    15421 gctcgtccca ttccacatag gtggagtggt gcatttttgc ggcaatctcg ctagtggcca
    15481 ttgacgagag cagagtgtcg gaactaccgt gcatcagaag tgtggggatg gtaatttgtt
    15541 cggaatggtt catggcatac tcagcggccc tctccatggc aaggtagagc cgaggggtaa
    15601 ttcgcctatg aataagcgga tccgtagcat actcctgtac caccttcatt tcatgcgata
    15661 cctgttcggg tcgtattcca gagttcatgg gtagaaaagg taagcataaa tctaagagct
    15721 taaccagact ttttaacgca gggttgattg gacttaccag cttcaaccag ggtgaagtaa
    15781 caaccagcag gttgattttg gatggtcttc ttattgcgta gttcagcgca atgttgcccc
    15841 ccatgctatg gccgtaaagc actaagggaa ttgaaccaat ttgcctgttg aatttattta
    15901 tgatgaaatc tacttcttgc aggaggtgct cgtagtttgg aacacatccg cgttgaccgc
    15961 ttgattgccc atgaccaaca tgatcccagc agtagaacgc gaaacctgca tcagcgaatt
    16021 gttttgccca ggtctggtag cgcatggaat gctccccaag cccatgaaca agtagtactg
    16081 cagccttagg gctggatggt tgaatatacc atgtaaacag cctataaccg ttagttttta
    16141 accattcctg atgaacagat tccacgttga ttagcgcagt tgaatttttc aaactgcaat
    16201 atagctaaaa acgtgaattg tttgcaggaa gagttagtaa agttaaagca gaaagggaac
    16261 aataataagg aatgcttgtg ttgaagaatg agatgtatgc tggcaatttt gagaggttga
    16321 aaaccagcct tttacctgtc aaaggaactc tccctgaact tgtcgaaggg tggaattagc
    16381 cagcaggatg agaagtttat tttatccttt atcatttctc atctgaaatc cgccggtagg
    16441 caaacaagtt cttgaatccg tcaggagccg gacaagttca gccggtaggc ggacaagttt
    16501 ttgaatcctg cttgaatttt tttatccttt atccttatta tttcatttgg ggctattgtt
    16561 cctgatggtc tggggatcaa gggcatccct aaggctgttg cccaacaggt taaacgccag
    16621 gaccagcatc attatggcaa aaccgggcag tatagccagg tatgctttat ccatgatgat
    16681 gtagccgtag tgatcgcgaa tcattgagcc ccatgagggc atgggaggtt gaacacctat
    16741 tcccaaaaag ctaaggcctg cctccagcaa aattgcagta gcaaagttgc tagcacatac
    16801 aattgccact ggccccataa tgtttgggag tatgtgcctg aaaattaccc taaacctgct
    16861 taatcccaga acggttgctg cctcaacata gccattttct ctgaccgaca aaacctgccc
    16921 cctcacaaca cgagccatat caacccacat gctaaggcct actgccacaa atatttgcca
    16981 gaaaccttta ccaataacca ttgtcagggc gattaccatg agtaaagtag gaactgacca
    17041 aaccacgttg acaaaccaca tgatggcgct atcaacccaa cccctcagaa atccggccag
    17101 cgaacccagt agtattccaa taagcaggga tatggcaacg gcaataaatc caactgacag
    17161 ggaaactctt gcaccaataa tcatcctgct cagcaggtcg cggccaaacc gatcggtgcc
    17221 cagaatatag gtgtgggtaa caaaattctc cttctccacc ctttcctgca gctggcttac
    17281 cgtcatagct gttgctcctc catcagcttg aacaaaaagg acagaatcgt tacggattat
    17341 cggcttagca ggtggttcaa ttctatacgc cacctctgca agagaaaatc tggctacaac
    17401 aggttccgat tccggaatgg gattataccc ctcaatcaca atgctggcat cttcaaagta
    17461 aaagctatta atgggaattt ccctataatc ggactcatcg ccatagagca ttctcccaaa
    17521 ccatgagcgg tgctgaatat cctgttgtct tctaacttta agcatggtaa cctcagctcc
    17581 tggtttgcag gatgacagct ccaaatgctg gttgttggcg tagggtgttg gatcgggagt
    17641 aatcaggtat cctaatatgg aaatcagcac aacaatcccg atcaggcaaa atgaggcaac
    17701 agcaatccag ctggctttca accgtcggat ggctagctgg atgggtgagc cactcatggc
    17761 tagttcttcc agcttactgc tcttcctttc caattttgtc ctccggcaaa accagctatt
    17821 gcgaccgtgc ataggtaaat ggggtaaatc agctgtaaag gtaaaaaaac aagtatcagc
    17881 ttcagctggt ttgtttgttt caggtatatg aagagtaggc aaaagttagc cagtgaggca
    17941 accaacaagc tgccaaccag taggttccat gcctctagat taaagatgca aatcatcggt
    18001 agaactagca accaaaggtt aaaaaccaaa actatcagcc ccacaagaat ggtttcgata
    18061 tcggtgtagg caggggcttt ggatgcccaa cggcgacgac gtcgaattgc cccaacaaaa
    18121 cctccgtcaa aacgggttag tacgcttccg gcgagattag tgctgaatag catgttgttc
    18181 gagtggtcct tcttaacctc gtggagcagg aacatatcat cacccgatgc aacatcaaca
    18241 cggagcgttt caggacgcac gttaatcata tccttacgaa aaacgaggtt agcggaagct
    18301 gcaactacag ggcgctttag cccgaacgac cccgccgaaa cagccattag cgaggcgtaa
    18361 tccaacgcct caaaccagga gaggaggttt gaattccttt caactaaaac gcaaccctgt
    18421 agcaaggcat acccattggc ccttcgggaa atatcgttaa gccatgtagc agcaggaaca
    18481 caatcggcat cagtgatagc taccaggtta aacctagcca attcaacccc aaataacaag
    18541 gcctgctttt tcccggtaat gcccttctga agtttcacaa gccgtaaccc cggcaggtta
    18601 tgaaatgatt gtgcaatttt atcaccccca tctgttgacc catcatcaac catgattagc
    18661 tcccacttgc ttgcatcaag ggtttgacgg cttaacgcct caagcaatcg ggtaaggttt
    18721 ggagcctcgt tccgaaacgc cacaaccacg ctaaccggtt gagccagctg gccaaatgct
    18781 tggttgtggc cagctgacgg ttggactaac ccaacaatca gcagcacaat caggacaacg
    18841 tagagcaggg tgatggcaat tgttgcgata gtaagcatga agcaaaatgc cttgtactgg
    18901 gttaaaatta gtgcaaataa aatgagaaga agctaaatgc gtaaaaaata tgatgagcta
    18961 aaattttgcc cggagtttgt ctaaggaact tgcccttagc agtaaggcaa acttactata
    19021 acacctgtcg aaaggagaat ccgccagtag gcggacaagt tccgtcagga gccggacaag
    19081 ttccgccagc aggcggacaa gtttatctta tcctttaccc ctgttcttcc ccatcctttt
    19141 caaggatctg aagcaatttt ttgtattcgt tggccttgct gcgattaccg tttctctcca
    19201 gagagtaaat caggttgaat accagctgtg aaattgttgt tgtgttggca catggcagaa
    19261 agtattcggg tttaggctcc agctgctgtc ggctaaggaa tagctctatc tcgttacggc
    19321 tgaaaaccga accgttgttg aatgggttga tgtagaacag gatactttca ggatccttac
    19381 cttttctttc agcgggcacg cggtacctgt cctgataggc aagcacaaag tttttaggaa
    19441 ggtttacgca cgaaatgggc aaccctaaac gctgggcaat ctcagcgtaa agaatggcga
    19501 gcataatagg accacccctc ttggtttcta ccacatggtt gataaggtgg ttgccgggga
    19561 ggaagaagtt ggatgtatta ggttcatact tatgaatggt aaagattaca tggttaatca
    19621 ccctgacctt ttccagtgcg gtgaggttgc tgtttatttc gagccagata tccttttgaa
    19681 gcttcttaat ctccaattca atgggctgga gcgataaatc gggaaactgc agacgggcta
    19741 ccagcactgc tccctctagt agattgttac tttccgaatc tttccactgg gtgagagcgt
    19801 tggcaaccgt gttaaactga atggtctgaa tcagattctc cagcctttcc tgaaatatcc
    19861 tgctgtccga cgacccccat gcaccctcaa gcagcggaac aacggtaatg ccctgcgtga
    19921 tgattttttc cgacacggcg ttgaaaatct cggggtcggg atcgtcaagc agggtaatga
    19981 gggagtgtat gtagcttttg tccaccactt gcagtgtgtt aaatggatgc aagcctgttc
    20041 agcgtgttct ctatcagatt tttcatggcc agctggtagt caaccttggc atcctttact
    20101 acccggttgg caatgatggt gcagatggta acggaatcat ggccaaggag cagcgaaagc
    20161 ccgttgattg ccgagctttc catttcaaag ttggtaatct tatagccctt atacctgaaa
    20221 tctacaattt tatcgttgag cgttggatcg gccaacggga gtcgcagtac tcgtccctgc
    20281 gggccgtaga aaccaggcgc cgaaatggta actcctttca cggttatacc ggcaaatagc
    20341 tcaaccagct tgcgggacga atggcagaag tatggtttag cacaaagcgg attccactgg
    20401 gtatgcttca ggaaggcctg ctccatttcc aggtcgcata ttccattccg gttggcatag
    20461 aagttcaaca gtccatcgaa accaatgctt atctcggtaa gcaccgagct gccgagctct
    20521 atctcgggtt gaagcgcccc cgatgttccc agcctcacca gggtgaactt ggtgtggttg
    20581 gccttgactg ttcggctggc caggtcaata ttggccaggg catccagctc gttcaccact
    20641 atgtcgatgt tgtcggttcc tattcctgtt gaaaccacgc ttatccgctt gcctttgtat
    20701 gttccggtgc aggttttgaa ctcccggttc gatacggtga gctcaacccg gtcaaagtac
    20761 gaggaaaccg tttcaaccct acccggatca ccaaccagaa ttacagtatc tgccagctgt
    20821 tcgggtttaa ggtgtaggtg gaagacgctg ccatcctggt tcagtatcaa ctccgacgac
    20881 tcaattactc tcatagctac tagttttgct gccaaaaata ctaaaaatcg ttcattttat
    20941 ggtctaatag ctacaaagac ttaacgtttt ttttacatgt catccattgc ataaaaaatt
    21001 gaactattcc tggcatgcac attccatttt gtatcaaaaa atgccagtat ctatgttatc
    21061 aacacctaaa tccttaggac ggttagcagc gtgttgatag cctgtttgca gcttgtttat
    21121 aattctaatg tccttgtggt ggtgcggggt tcattgggca attcattccc cccgctactg
    21181 caaaagtccc ctcgtgtggt ggtatagaga aagaaagtag tgggcaggaa ttagcaaaag
    21241 aataatccgg ctggactata cggtatatac ttaaagatag acgtggtaag cgttgttggc
    21301 aggcagggat gggtttgcca cacgacagga gttgtgcggc agcaatgccg aagcgggtaa
    21361 agctggtaaa agctgcatat cccttgtaaa ttcaaccata cacaaagccc cgggtaaccc
    21421 aagctctact ttaatgcccg accaagctca acactgtcct gcagcaagcc cctagcttcg
    21481 cccgaatttc cgactcccga cttgttgcaa atcgcattgg aacgctgcag gtgccactgg
    21541 gaatagaaaa gccgttatca tcattattaa cacgcagacc tctatagcgg ttagcagcgt
    21601 gttgatagcc tgtttgtaac atgttgatag cttattcttc aggatggcgg agcaggagta
    21661 atggccgtta tcagcagcca aaatttcccc cagcctccac ccgggctctt tagcaataga
    21721 aaaaaacttg acacgagctg gaatatgccg tacaattgca taaaaaattt gccggcaggt
    21781 taggccggat aaaaattata ggaaatgaac tttagctgac aaaaaaaaat caacctttgg
    21841 gctacgggag atggcctgtc ggttgcggga aaagggtaag gcgcttcccc tcatatatca
    21901 aaatattttc taatgaatcc cttttttagt ttatttcatt gtgaagaaca gaaaaaaact
    21961 cgttacaatt ttttccacct tcagggtcta tcgctaggtt tttcgaatag tttcaagcta
    22021 ctaatgagta taagccccaa aaaatgaatt tatgaatagc atcacgaaac gttgccacat
    22081 agccctgctg ctcattctaa cggcttctcc tgcgttttgc cagagtggcg ataccgtgga
    22141 ggcgaagcag ggggtgaagg tagagctggg ctacatggtt atttccaata gccttaacgc
    22201 tgacgattac catgatttca aatacatgca tgttattgat aatgcaaatg cttttattag
    22261 gggagcaaat ttaaaattta cctttccaac caggtggaag tacctggatg ctacggccgg
    22321 ggctctcctc attagatgtt tagatgaaag tggcactagt gcaattgatg taagccatat
    22381 caatgctgat gcatataatc ttaacggggg cggtgtttac ctgggcatca gccccaagct
    22441 gaaggggaaa cactttgggt taacctccga ccttgcagtg ggggtgctgt cgttcaagga
    22501 gtacgtgagc attgtaatta atgatcgtga gcctaacgtg gacgaacaca acctaaaggc
    22561 ctcctacggt ctgggtgcgg ttagctcggt tggcttctac gtcagcttct ggaggatggg
    22621 catcaaccca tccctaacct gtattttctc gggtggagcc agctccagct ttaccttctg
    22681 gggcttgaac attcccatta ccatccaatt ctaaaaatac gtctgcctta tgtcgtggag
    22741 tagccaggct gcaaagtccg gctactcttt ttacccatcg ggttgaagta atctgaacaa
    22801 ggggagcagc acacaacagc agttcgttat caaacgtccg ccataaaccg tcatcagccg
    22861 gacaagttct tatatcttga attttatatc cgccggtagg cggacaagtt ccgtcaggag
    22921 ccggacaagt tccgtcagga gccggacaag tctgaaccct gagcagttcg acaaatgtta
    22981 tagtgagcca gccgaact
//
LOCUS       DAOPHX010000026        22397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_2436_length_22397_cov_5.730687, whole genome shotgun sequence.
ACCESSION   DAOPHX010000026 DAOPHX010000000
VERSION     DAOPHX010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 22397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22397
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2436_length_22397_cov_5.730687"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="PK502_03335"
     CDS             <1..1443
                     /locus_tag="PK502_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPG99881.1"
                     /translation="KSIPAEMAPMEDRSQVSLSVTFPEGTTYDYSAAYVEDVAALVDK
                     LVPEAEKATSRAWRGGASMDVVLIPPSERDRSQQDIANMLTAELRKKTKARVNVIQQS
                     TFGGQRASLPIQYVLQAQNIDKLREILPAFMAEVQDNPTFTMADVNLRFTKPELQVEI
                     NRDKANMLGVSTQNIGQTLQLALSGQRFGYFIMNGKQYQILGELPRATRNEPLDLKSL
                     YVRSDAGQMVQLDNFVEVKESTAPPQLYRYNRFVSATISAGLAEGKTISQGIEEMDKI
                     AAKTLDDTFRTALAGDSKDFTESSSSLMFAFAMAIVLIFLVLAAQFESFKDPVIVMMT
                     VPLALAGALIFMWYFNITMNIFSQIGIIMLIGLVSKNGILIVEFSNQRKNAGMSKLDA
                     VKYAAAARFRPILMTSLSTILGILPLALGLGEGAQSRVAMGTAVVGGLTVATFLTLYV
                     IPGVYLLISSESAAGKAANSKGREVEGIEQ"
     gene            1555..2931
                     /locus_tag="PK502_03340"
     CDS             1555..2931
                     /locus_tag="PK502_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPG99882.1"
                     /translation="MNGKFDLMNQTTPSALAAPMAKRIFTITLLICLCSGLSKSQELF
                     DLSSCIKAGLEQNFSILIARNQEKIASNNYTVGNAGYLPTLSGSGRYGGTINTTHQSF
                     TDGTESEANGVNNTSGVVGVDLNMTIFNGFKVKATYQKLNEMKQLGILNTQLEMENLA
                     AEIASEYFFFIQQLNLYNNMAYAVELSHERVRIDEERYLLGSSSKVDLLQSIVYLNSD
                     SSRFAKQKEVLRASQVRLFKLMGNVDLGYDIRPKDTVISINDGLLYDDLLASTMDQNT
                     SLQIARRNTTISQYDYKIIASRSYPYLNLTSGYDYSFNDYETGTVKSQQIHGLSYGLT
                     LGVDIFDGFNRKRERTNAAIEIENREHEYSDVVQGVKADLLTIYYAYQNNLKLLKLEE
                     QNLSVARENLEIAMERYRLGDLSGLELREVQKSLLDAEERLISVKFQAKLAEISLFQI
                     SGKIMSYL"
     gene            complement(3174..3944)
                     /locus_tag="PK502_03345"
     CDS             complement(3174..3944)
                     /locus_tag="PK502_03345"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPG99883.1"
                     /translation="MSFIQSIIIAIVEGITEFLPVSSTGHMILASTLMKIQENDFVKT
                     FEIFIQLGAIMAIVMLYAKKFLQSFSIYVKLGIAFIPTAVVGYIAYDFIKGYLFNSIV
                     VAVSLIVGGIVLVLIDQKLTTKKSKVEVVEDISYKNSFFIGLIQCFSMIPGVSRAAAT
                     IIGGVFNGLDKKQATEFSFLLAVPTMIAASGYDLLKTPISFTSHEVVLLATGFVVAFF
                     TAWLAVKVFLKIVENYGFKYFGYYRILVGAIFLLFFRF"
     gene            complement(4211..4846)
                     /gene="msrA"
                     /locus_tag="PK502_03350"
     CDS             complement(4211..4846)
                     /gene="msrA"
                     /locus_tag="PK502_03350"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HPG99884.1"
                     /translation="MKRVAIVASWLLITQFSMACESQTNQNHKEMKASASETITLGAG
                     CFWCVEAIFSRIDGVLKVESGYSGGSAVNPTYREVCNGNTGHTEVVQVTFDPEVIPLA
                     KILEIYFKTHDPTTLNRQGADVGFQYRSVIFYHSQEQKKIAEEIKAELEKARIWNSPV
                     VTAIEPFKNFYKAEDYHQEYYENNKNQPYCRMVITPKVDKLEKVFADYLKH"
     gene            complement(4978..6168)
                     /gene="mnmA"
                     /locus_tag="PK502_03355"
     CDS             complement(4978..6168)
                     /gene="mnmA"
                     /locus_tag="PK502_03355"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPG99885.1"
                     /translation="MKKTEMERVVVGLSGGVDSSVAAYLLKEQGYEVVGMFMRNWHDT
                     TGVLKGNCPFDDDRDFAEMVARKLRIPFEFVDLSNEYRQRVVDYMFNEYSKGRTPNPD
                     VLCNREIKFDAFVKAAFELGADYVATGHYCRKQEIEVNGQTYYRLLTGTDSNKDQSYF
                     LCQLSQQQLSRALFPIGHLLKPEVRKIASELGLATAERKDSQGICFVGKVDLPVFLQQ
                     KIKAVDGDIVEIPRAFYSSQPVISPDDLEALCKPTFYSKADGNLVGQHHGAQFFTIGQ
                     RKGLNVGGKSEPLFVIATDVLQNVVYTGMGDDHPGLYRKGLFVSQNDVHYIRPDQKLR
                     PGQTARLMVRIRYRQPLQKATLLSRDNGIYIIFDNPQRGVTAGQFAAWYDNEELIGSG
                     VIAM"
     gene            complement(6293..8539)
                     /locus_tag="PK502_03360"
     CDS             complement(6293..8539)
                     /locus_tag="PK502_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG99886.1"
                     /translation="MHNLRHLSYLYFLILIFFPTKSFSQDWNLLFTLETDAEFYMADG
                     NYSRAADTYLKALKKYPESCNLMFKVGYCLLKTDDRKAEALSYLEEAAKKVSDKYDAR
                     SLKEANSPPEVLYHLGISYMHQNNFDKAKAQFEAYKKYVNPNDAYVLQQVEQNIKSCE
                     TAKAMMDSPIGLNLNRLEEGVNNDQSNFDAVLSGDGKTMAFTSRSTSGNKVFISFMDK
                     DKWSTPKDITRNLGSSYLTTSFLSYNGEELYLIDNDLKNSEIVVSFLQKNKWSNPVKA
                     AKPINSKANETHVCVTRDGNTAYFTSDRKGGQGGFDIYKVTISGDKWGEPVNLGPNIN
                     TPLDEATPFLTPDEKYLFFSSQGHNSMGGYDIFYVNLEGSSNVKNIGYPLNTPDDNLF
                     YFPTSLTSGYISTFSPNGLGGLDICSVEIIPQVDVKINIMLADKAPADKSYTVSIIDT
                     KDGKPVSSVNQNGKKQILQKLSPGTFTVAASGVEFDSTSSLLEIPSKPDKNEYQISLL
                     LNPSQPKPEPVAEVSQPANDIPIQKEVKPVPVVVEEPKPEKPVPASVEKPKQEKSTAA
                     SVPVNPSPKPEVKKREMEKPKPVHVAVTNLSLAAGTSTSLAHPTYSVQLMACKQPVDY
                     DYFKLDSIFVSISPEGYYRYSVGYTLNVQQAEELLARVKTLGYTNAYVRINKEQPSYT
                     IQLMALTKPKRLNQLNVIGSTMVYRGCDGIYRYCTGSYSTPEEAQNDLANFKNAGYSD
                     AFIRHLGR"
     gene            complement(8855..10036)
                     /locus_tag="PK502_03365"
     CDS             complement(8855..10036)
                     /locus_tag="PK502_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPG99887.1"
                     /translation="MSEKFFIEVNCQQKNHDGERICGDVFYSKRLQDEERTIIVLSDG
                     MGHGVKANVLATLTSTMAFNFTREHKEVNTIAEIIMNTLPVCSERKMSYSTFSIVDIE
                     HNGDTRILEYDNPECFIMRGTEPLIPEWQCIILNSEQNAGKELRYCSFTPQKEDRIIL
                     WTDGVSQSGMGTPDYPFGWSDGAREFVEKMVKNEPDISARKLSAKVVNMAYVNDGYHP
                     KDDTTCATIYFRHPRKLLICTGPPFEKENDSMLASIFNSYEGKKVICGATTVDIIARE
                     LKREVVDSMEFTDPDLPPTSQMDGADLVTEGILTLSKVNTILDSYNETVTLGNGPAEQ
                     LVKLILESDIIDFVIGTCINIAHQDPNLPVELEIRRTVVKRIANLLQEKFLKEVKLKF
                     L"
     gene            complement(10063..11943)
                     /locus_tag="PK502_03370"
     CDS             complement(10063..11943)
                     /locus_tag="PK502_03370"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPG99888.1"
                     /translation="MSQLFEISEETCINCYACVRVCPVKAIEVKAGQDFARILQNRCV
                     GCGSCLNICPVNAVSYRSSKKNVLELLKGNEPVAAICSPSISGEFVDITDYRKFVQMI
                     KTLGFTYVCEVTFGVDLVAREYKKLFDDFKGKYFITSMCPVVVSMVEKYHPEMVNNLA
                     PIISPIIATAQAVHNQYGSNVRIVHIGPCIQSKEEAKLYNGKSQIDEVLTFVELRQLF
                     EEFNINESNLEYSEFDEPIGYKGSLYPIGRGILQAVDISEDLLTGTVISANGPNNTLQ
                     ALEQFETSAEMINRHFNLFYCEGCLMGPGTSKGGKKFIRRTMVVNYANKRLLTFNRKK
                     WEENISRNEKNSYTRDFTPDDQRLPLPSQQKLEEVLKVIDKEFVGKETGCEACGYASC
                     YDFASAVASGLAHTDMCLNFTLKNRQEYIKSLRSTNEKLAKTQEALRESEKKARIEQE
                     AAREASETVTTMLKKLPAAVVLIDKKLKIIQSNQSFIDLLGDDALEINEIVPGLVGAD
                     LKSLVPYNIHNLFTYVLQNNSDILNRDVHYRDKLLNISVFTIRPNSIVGAVIRDMYAP
                     AVQKEEVVKRVTEAIDRNLAMVQKIGFLLGEGASEIEQMLNSIIQTYNEGVAKAENSK
                     TK"
     gene            complement(11962..12213)
                     /locus_tag="PK502_03375"
     CDS             complement(11962..12213)
                     /locus_tag="PK502_03375"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPG99889.1"
                     /translation="MEKKNEIVICLGSSCFARGNKATLKTIQKYIDDHHLREKVYFHG
                     GHCFGRCAEGPTVTVNGKIFTGVDEYNILDILNSELSEY"
     gene            12493..12954
                     /locus_tag="PK502_03380"
     CDS             12493..12954
                     /locus_tag="PK502_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPG99890.1"
                     /translation="MKRSIEAILEQYDHAGMDSLIPILQEIQNKTGCLNEESIVMVGK
                     HLGIPTSKVYGLATFYNQFKFEPKGKFHILVCNGTSCRLNGSKWIIDFIEKNLKIKNG
                     ETSRDGTFSLEVISCMGACESGPLISINEEYYCNLTPAKLGEVLAGLAESE"
     gene            13054..15000
                     /locus_tag="PK502_03385"
     CDS             13054..15000
                     /locus_tag="PK502_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPG99891.1"
                     /translation="MNHSYTLADKTFFCEQVLPLLHAELNSDTQKHLSTYRHERVKKT
                     VIYIGITTSSVVAGALKTLKAIEGYLEENSVDAIIVPVGSYGLCSFEPIVEVQLPGKK
                     RVAFRNVTYDRVEFLLDGAFNSFVQPDTALFQHNSDILEPWPDIPVKDDFPFFAKQDR
                     LLLRNIGIINPENLLEYISFGGYRALAYTLRNQTSDSVCRIVELSGLRGRGGGGYPTG
                     LKWRKTLSVAATERIMICNADESDPGAFMDRLLAESDPFRIIEGLTISAYAIGANKAI
                     IYIRNRNKLAVDRLTLAMEQAYDFGLLGYNILDSGYSLDISIFQGPGAYVCGEETALI
                     QSLEGKRGTPSIKPPYPNEVGLNGKPTVVNNVETLASIPDILLNGADWFMKIGTATSK
                     GTKIFSVNGKASLTCVVEISMGRPLSAIVELAGGVKPDCTLKAIHLGGPSGVSVTPDE
                     LYLPVDFDELKVHGIPLGSGGVQILDQSVCMVDLTKFFMHFIRNESCGKCIPCREGSS
                     RMYQILNEISRRPDSSDKHHTLERFKGVIQLETLAEVMHDTSLCGLGQTASKPLLKAL
                     KAFRDEFEEHIFDRSCRAGVCRDLRTFYIDTEKCTGCAACAKKCPTNAIIGTPRSPYF
                     IVEDKCTGCGSCMEVCKFASVFYR"
     gene            15151..16875
                     /locus_tag="PK502_03390"
     CDS             15151..16875
                     /locus_tag="PK502_03390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HPG99892.1"
                     /translation="MNFNIEVNNRQILVHRGETILNALRGNGIKIPTLCNMKELSPTG
                     ACRLCVVEVEGRERLVTSCSQLVEEGMRIKTHSSRVVKARKTLVELLLANHPDDCLYC
                     ERNGRCELQSLAVELHVRERHAQNKTFSQKLDLSSHSIIRDPSKCVLCGRCVRICNEL
                     QSVSTFEFVKRGCNMYIGTAMSRDLTFSNCIACGQCVMSCPTGALSERSNVDEVIDAL
                     NNPNLIPVVQVAPSISVSLASELGLKPGKDLNGVLNAILRKIGFRYVFDSAAAADIAV
                     METATELLKRRENGEKHPLISSCCPAWVKFMEQYYPEHLSWLSTTKSPQQVMGALIKS
                     YFAPSYGINPAGIFSVSVMPCLAKKIEARREEMTTKGVSDVDTVISTRELVQLVELYG
                     IDVQNVESQQPDSPFDIRSSAGKLFGVSGGSSEALARTLHYMNTDTELDNFKIAELRS
                     SSGIREFKLTTGKQEFVFVVLSGMKSIHSFMAELINGNVRADYVEVMACPGGCVNGGG
                     QPFAMDPKEIRQRTKSIYELDEADVVKSSHKNPVVKSVYDDFLKEPCGELAQKFLHTS
                     YYKRDVLL"
     gene            16954..18942
                     /locus_tag="PK502_03395"
     CDS             16954..18942
                     /locus_tag="PK502_03395"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPG99893.1"
                     /translation="MQEENRKLVYTIKELCRVCYTCVRECPAKAIRIINGQAEVIAER
                     CIGCGNCVKVCSQDAKVYLQTRMEVRDLLQSEDKVAALVAPSFPAEFSEIDDYRKFVG
                     MLRQLGFSYVFEVAFGADLVARRYKELLEDRSGKGYISSDCPAIVTYIRYYHPDLTEY
                     LAPVVSPMIAISRVVRKKLGNGVKLVFIGPCIAKKAETDEVDEALTFRELRGMLEYAG
                     VTPEAATPSEFDLPRAGRGAIFPVSRGLIQTVRVNEDLVEGNVTVAEGRMGFQEAIKE
                     WEIGNLKGQNLELLCCDGCIMGPGMSPGGKRFERKTQISRYARQKLSELEHDEWLYSM
                     NEFRDIDVSRSFQRDDRRMPIPSDDQINRVLASMGKFSPRDQLNCGACGYETCVEHAI
                     AIIDGLAEVEMCLPYAIEELHISVDNLAISNEKLAKMQQALKQSEKLAHMGQLSAGIA
                     HELNNPLGVVLMYSNILMDECKPDDPLRKDLELIATQAERCKKIVGGLLNFARKNQVK
                     VERVDIRKLAAESVTSVVIPKTISTQVVCKANNTEAYLDYEQMMQVLTNLNKNAVDAM
                     PNGGVLTVEVDENESDVIFRVSDTGTGIPEENMDKLFTPFFTTKGTGKGTGLGLATTY
                     GIVKMHSGKIEVDSNTDPAKGPTGTTFTIVLPRKPTLG"
     gene            18970..19368
                     /locus_tag="PK502_03400"
     CDS             18970..19368
                     /locus_tag="PK502_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPG99894.1"
                     /translation="MPTPAKKTILIVDDDVDYLYQLKFQVEKMGFETVTAESQHEAET
                     LLNTIKPDLAILDLMMESEDSGFILSYKLKRKYPDVPIIIATAVSAETGMTFGINTEE
                     ERKWIKADLYLEKGIRPDQLHREIVKLLKL"
     gene            19375..20511
                     /locus_tag="PK502_03405"
     CDS             19375..20511
                     /locus_tag="PK502_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HPG99895.1"
                     /translation="MEVVDVLVVDDEPGIRSGVSRILKNYTINYPFLEDDISFNVVEA
                     ATGEEAIEIIQRKPPAIVLLDNKLPGIQGIDVLQFISAHEPNILVMMITSYASLELAV
                     KATNIGAHDFVPKPFTPQELRSSIENITKHYFLRQMTRKLHREGKQVRFEFLTMLSHE
                     LKAPLNAIEGYLRMMQEKQLGESLDDYQEIIDRSLSRIQSMRNLIMDLLDLTRVESGK
                     RERDLQDIDLTLVARLVVETMMPMAIQRDVTLTLESDEPVVVKADADDMEILLNNLIS
                     NAIKYNVEGGWVKCKFSKTPTYATCSVSDSGVGISADDIPKLFHEFTRIKNPLSRNVS
                     GSGLGLSIVKKIADQYGAEIKVESTLGKGSTFTLQFPVSAENPA"
     gene            20785..21408
                     /locus_tag="PK502_03410"
     CDS             20785..21408
                     /locus_tag="PK502_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPG99896.1"
                     /translation="MKLPEKTVERLSEYRRTLLSCIAKGKTHIYSHELAGIHNITAVQ
                     VRRDLMLIGYSSIKKKGYDTQELADVIGALIDHEKGLNVAVVGMGNMGRAITSYFNGK
                     RPKLNIVAAFDVDPNKVNRVIAGVNCFHMKDLLEISKRYEISIAIITSPPDSATQVAE
                     QLVSAGIKGILNFTTIPLNVPDTVYLEEFDMITSLEKVAYFVKTKDE"
     gene            21444..22388
                     /locus_tag="PK502_03415"
     CDS             21444..22388
                     /locus_tag="PK502_03415"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HPG99897.1"
                     /translation="MQVHYGFDNVGYIKNPVVTTGSFDGVHIGHKAILKRINDIARGI
                     DGESVLITFYPHPRKVLYPENAGKNLVFILSQKEKIELLSKTGLDHLIIVRFTLEFSH
                     ISSVDFIQHFLIAKLHARFVVVGFNHHFGHNREGDFEELKRLGLQYNFGVEEIPEQDI
                     QQETVSSTAIRKALLEGKIQRANAYLDHHFIIMGTLGKGAHIFQRAGFPTLTVQIEEA
                     GKLIPHEGIYAVTLEWNSTHYRAMAIIWLDNYGSNEPVLGPLNVDVHIMEFDNILSED
                     DAKLFFQKQLADHVDIADEERLASQLSAACSSLDELIF"
BASE COUNT         5925 a         4856 c         5243 g         6373 t
ORIGIN      
        1 aaaagtatcc ctgccgaaat ggctcctatg gaggatagat cgcaggtaag ccttagcgtt
       61 acattccccg aggggacaac ctacgactac agcgcagcgt atgttgagga tgtggctgct
      121 ttggttgata agctggttcc tgaagctgag aaggccactt cgcgtgcatg gagaggaggt
      181 gcttccatgg atgtggttct gataccgcca tcggagagag accgctcgca gcaggatatt
      241 gccaacatgc taaccgctga actcagaaaa aagaccaaag ccagggtgaa tgtaattcaa
      301 cagtcaacat ttggtggaca gcgggccagc ttgccaatcc agtacgtgct tcaagcccaa
      361 aacattgaca agctgcgtga aatactccct gctttcatgg ccgaggtgca ggataatcca
      421 acttttacca tggccgatgt aaacctgagg tttaccaaac ccgaacttca ggttgaaatc
      481 aaccgtgaca aggccaacat gcttggggtt tcaacccaaa atatcggtca aacccttcaa
      541 ctcgccctta gcgggcagcg ttttgggtac ttcatcatga acgggaagca ataccaaatt
      601 cttggcgagc tgccacgtgc tacccgcaat gaacccctcg atttgaaatc gctttacgtt
      661 cgcagcgatg caggacaaat ggtacagctc gataattttg ttgaggtgaa ggagtcgacc
      721 gctccccctc agctttaccg ctacaacagg tttgtttcgg caaccatatc ggccgggctg
      781 gcagagggaa agaccattag ccagggaatt gaggagatgg ataaaattgc cgccaaaact
      841 ctcgacgata cttttagaac agctctggct ggtgactcga aggacttcac agaaagttca
      901 tcaagcctta tgtttgcatt tgccatggcc attgtgctca ttttccttgt tctagctgcc
      961 cagttcgaga gctttaagga ccctgtaatc gtaatgatga ccgttcccct tgccttggcc
     1021 ggagctttaa tcttcatgtg gtactttaac atcacgatga acatattcag ccagattgga
     1081 atcatcatgc ttattgggtt ggtttcaaaa aacggaattc ttattgttga gttttcgaac
     1141 caacgtaaaa atgccggcat gagcaagctt gatgctgtaa agtatgctgc tgcagcacgt
     1201 tttcgtccta ttctgatgac gagcctatcg acaattttgg gaattctacc cctggcgtta
     1261 ggtctgggcg agggagcgca aagtcgcgtt gcaatgggaa ctgctgtggt tggaggtctt
     1321 accgttgcta ccttccttac actttacgtt atacccggtg tttacctgct gatttccagc
     1381 gaatctgctg caggtaaagc ggcaaactca aaagggcggg aagtcgaagg tattgagcag
     1441 tagccgctaa tttggttaat gttttgatgg ggtttccaca cttgtacgta gctactttta
     1501 tagatttgca ttctgaaacc aattgatatt caaactgata aatattaaat actgatgaat
     1561 ggaaagtttg atttaatgaa ccaaacaaca ccctcagcat tggcagctcc aatggccaaa
     1621 aggattttta ctattaccct tctgatatgc ctgtgtagcg gcttatccaa atcacaggag
     1681 ctattcgatt tgagctcatg cattaaggct ggcctcgagc aaaatttttc tatactgata
     1741 gcccgcaatc aggaaaaaat tgcctcaaat aactataccg tagggaatgc cggatacctg
     1801 cctacgctca gcggctcggg aagatacgga ggaactatca acaccactca ccagtcgttt
     1861 accgatggaa cggagagcga ggccaatggt gtgaacaata catcgggggt ggttggggtt
     1921 gacctgaaca tgaccatttt taacggcttc aaggtgaagg caacctacca gaagcttaac
     1981 gaaatgaagc agctgggcat actgaacact cagctggaaa tggaaaatct tgcagcagaa
     2041 attgcttccg agtatttctt tttcatccag cagctcaacc tttacaataa catggcatac
     2101 gccgtggagc tttcgcacga gcgggttaga attgatgagg agaggtacct gctgggttct
     2161 tcttcaaaag ttgacctgct gcagtccata gtgtatctta attccgacag ctccagattt
     2221 gccaagcaga aggaggtgct tagagcttcg caggtgcgac ttttcaagct aatgggcaac
     2281 gttgacttgg gatatgacat taggccaaag gatacggtca tttcaattaa tgatgggttg
     2341 ctttacgatg atttgctggc ctcaaccatg gatcaaaaca cctccctgca aattgcccga
     2401 agaaatacaa caatatcgca gtacgactac aaaattatcg cttcccgttc gtacccctac
     2461 cttaacctta catcgggata cgactattca ttcaacgatt atgaaaccgg aaccgtaaaa
     2521 agtcagcaaa ttcatggctt aagctatggt cttactcttg gagttgatat ttttgacggc
     2581 ttcaaccgca agcgcgaacg gacaaacgca gctattgaaa tagaaaatcg ggagcatgag
     2641 tatagcgatg tggtgcaggg agtaaaagcc gacttgctaa ccatttacta tgcctatcag
     2701 aacaacctca agctgctgaa acttgaagag caaaaccttt ctgtagcccg tgaaaacctg
     2761 gaaattgcca tggaacgcta taggctgggc gatttgtcgg ggcttgaact aagggaagtc
     2821 cagaaaagtt tactcgatgc cgaagagagg cttatctccg ttaagttcca agcaaagcta
     2881 gccgaaattt ctctattcca aatttcaggg aaaatcatga gctaccttta aatttcagct
     2941 tccagtgctg tggcatgatc aaagacgttc tagctgatcg attggcttta caggttgaat
     3001 tttgagttaa cctgttaatt atcaggataa aggtttggta gaatgctgct tataaagggt
     3061 gttagcagta acacccatgg aaaatacccg gtaggtatag ctatcctttc agagcatttc
     3121 ttgttttgta gagtctaagg ctattaccat gcaggggggg agctgctggt ttgctaaaac
     3181 ctaaaaaaca gcaaaaaaat agcgcctacg agtatccggt agtagccaaa atacttaaag
     3241 ccgtaatttt ctactatttt taagaagact tttacggcaa gccatgcggt aaaaaaggct
     3301 accacaaatc ccgttgccag caaaacaacc tcgtgagagg tgaagctaat tggagttttc
     3361 agcaaatcat acccagaggc ggcaatcatg gttggaacgg caagtagaaa cgagaactct
     3421 gttgcctgtt ttttgtcaag tccgttaaaa actcccccaa taattgtggc tgctgccctt
     3481 gacactccgg gaatcatgga gaagcactga attaatccta tgaaaaaaga atttttatac
     3541 gaaatatctt ccaccacctc aactttactc ttttttgtag tgagcttttg gtcaataagc
     3601 accagaacaa ttcctccaac aattagtgaa actgcaacta caatagagtt gaatagatat
     3661 cctttgataa agtcgtaggc tatatacccc accaccgcag tgggaataaa tgctattcca
     3721 agctttacat aaatggaaaa actttgcaaa aactttttgg cgtaaagcat tacgatggcc
     3781 attattgcgc ctagctgaat gaatatttca aaggttttta caaaatcatt ttcctgaatt
     3841 ttcattaatg ttgaagccag aatcatgtgg ccggttgacg acacgggtag aaactccgtt
     3901 attccttcaa caatagctat gataattgat tgtataaaac tcatcgtctt tcaggattgg
     3961 ttgagtgctt tttactttta gcctgcaaaa gtaggctcat tttccataat tgaatccttt
     4021 taccggaaat tttttgaaaa cggtatccct aagggttaat gccagcaggg tgtatttatg
     4081 atggatatgc ctacatggct gccatgcctg agggcatgat ttttttaccc gtttgctttt
     4141 ttagaaacgg aataaatgat aacgcaggaa tgttctggct agtggttccc cgtggagttt
     4201 ctttgccctt tcagtgtttc aggtaatcgg caaaaacctt ctcgagtttg tcaacttttg
     4261 gggttatcac catccgacag tagggttggt tcttgttgtt ttcgtagtat tcctgatggt
     4321 aatcttcggc cttgtagaag tttttaaacg gttcaatagc cgtaacaaca ggtgaattcc
     4381 atatccttgc tttttccagc tcagctttaa tttcctccgc tatttttttt tgctcctggg
     4441 agtggtaaaa aattaccgag cggtactgaa atcctacatc tgccccttgc ctgttgaggg
     4501 ttgtggggtc gtgggtttta aagtagattt ccaaaatttt agccaagggt attacctcag
     4561 ggtcgaaagt aacctgaaca acctcggtgt gaccggtatt accgttgcag acttccctgt
     4621 aggttgggtt tactgctgag ccacctgagt accccgattc cactttcaaa accccatcaa
     4681 tcctgctaaa tattgcctct acacaccaaa aacatcctgc cccgagggtt atagtttcgc
     4741 tagcgcttgc tttcatctct ttgtggtttt ggttggtttg actttcacat gccatggaaa
     4801 attgcgtaat cagcaaccat gatgcaacaa tggcaactcg cttcatcgtg tagtaatttt
     4861 aaccagtaaa cagctacatg ccttattttg ttttgcagca ggttggatta catagttatg
     4921 gccagcagct cgattcccta tttgaggtaa agggggtgag tgaattcggg ttgcaaccta
     4981 catggcaatc acaccggaac caattagctc ctcgttatcg taccatgcag caaattgtcc
     5041 cgctgtaacc cctctttggg ggttatcaaa aatgatatag attccattat ccctggaaag
     5101 cagcgtagct ttctggagtg gttgccggta gcgtattcta accatcaacc ttgctgtttg
     5161 tccgggcctt agcttttggt cgggacggat gtagtgaacg tcgttttggc tgacaaacag
     5221 acctttacgg tataggccgg ggtgatcgtc acccataccc gtgtaaacca cgttctgcag
     5281 aacatcagtg gcaataacga aaagaggttc tgatttcccc cccacattta agcctttgcg
     5341 ttggcctatg gtaaagaatt gagccccatg gtgttgtcca acgaggttcc cgtcggcttt
     5401 gctgtagaaa gtgggcttgc agagtgcttc tagatcatca ggagaaatca ccggttggga
     5461 gctgtaaaat gccctgggaa tttcaactat atcgccatca actgctttaa ttttttgctg
     5521 aagaaaaaca ggcaggtcaa ccttgccaac aaagcaaatt ccttgagaat ccttacgttc
     5581 ggcagtagcc aaacccagct cagaagcaat ttttcgcact tcgggcttga gcaggtgtcc
     5641 aatggggaac aatgccctgc tgagctgttg ctgtgaaagc tggcacagga agtagctttg
     5701 gtccttgttg ctatcggttc cggtcaatag tcggtagtat gtttgcccat taacctctat
     5761 ctcctgctta cggcagtaat gaccggtagc cacatagtca gcacccagct cgaatgcggc
     5821 tttcacgaag gcgtcaaatt ttatctcccg gttgcatagc acatcgggat tgggcgtgcg
     5881 accctttgag tattcgttaa acatgtagtc aacaacacgc tggcgatatt cgttgcttaa
     5941 gtcaacaaac tcaaagggaa ttcttagctt gcgtgcaacc atttcggcaa aatccctgtc
     6001 gtcgtcaaaa gggcaattcc ctttgagcac tcctgttgtg tcgtgccagt ttctcatgaa
     6061 catgcctacc acctcatatc cttgctcctt aagtaggtat gctgctacgc ttgaatcaac
     6121 gcctcccgaa agtccaacaa ctacacgttc catttctgtt tttttcagct ggcaaagtta
     6181 caaataagta cggtttggtg gcagtgcccc aaatggcaat gtataatact agggcggttt
     6241 cagggttaag cggtttggct gtgttggaaa attttttctg aacgggttaa ttttatctcc
     6301 ccaaatgcct gatgaaggca tcggagtatc cggcattttt aaagttagcc aggtcattct
     6361 gcgcctcttc gggcgtagag tagctaccgg tgcagtaacg atatattcca tcacatccac
     6421 ggtaaaccat agtgctacca attacgttta gctggtttaa ccgtttgggt tttgtgagtg
     6481 ccataagttg aatggtgtag cttggctgct ccttgttaat tctaacatag gcgttggtgt
     6541 agcctaatgt ctttaccctg gcaaggagtt cttcggcttg ctgtacgttg agagtataac
     6601 caacgctgta acggtagtag ccttcgggcg atatggaaac aaatatgctg tcgagtttaa
     6661 agtagtcgta gtccacaggc tgcttgcaag ccataagctg cacggaatag gtagggtggg
     6721 ctaatgatgt agaggttcca gctgctaatg aaaggtttgt aacggcaaca tgaactggct
     6781 tgggcttttc catctcacgt tttttaactt caggcttggg agatgggtta acaggtacac
     6841 tggcagctgt tgatttttcc tgctttggct tctctaccga agcaggaaca ggcttttcgg
     6901 gcttaggctc ttccaccact actggtacgg gctttacttc cttttgaatc ggtatatcat
     6961 ttgccggttg ggatacctct gcaacaggtt cgggtttagg ttgggagggg ttcagcaata
     7021 ggctaatctg gtattcgttt ttgtcgggtt ttgagggaat ttcgagaagg ctcgaagtag
     7081 agtcaaattc tacacccgaa gcagcaacag taaacgttcc cggggataat ttttgtaaaa
     7141 tttgtttttt cccgttttgg tttacactgc taaccggctt gccatctttc gtatcaatga
     7201 tgctaacggt gtagctttta tcggctggtg ccttgtcggc aagcattatg ttgattttaa
     7261 catcaacctg gggaatgatt tctaccgagc aaatatccag accgcccagc ccattgggag
     7321 aaaatgttga aatatagccg ctggtcaggc tggttgggaa gtagaaaagg ttgtcatcgg
     7381 gggtgtttag ggggtaccca atattcttga cgttagagct tccttccagg ttcacataaa
     7441 agatatcgta tccacccatg ctgttgtgcc cctgggagct gaagaacagg tacttctcat
     7501 cgggggtaag gaatggtgtt gcttcatcta aaggggtgtt gatgttggga ccaaggttta
     7561 cgggttcgcc ccatttgtcg ccgctaatgg ttaccttgta gatgtcaaat cctccttgcc
     7621 ccccttttct gtcgctggtg aagtatgcgg tgttgccatc gcgggttacg caaacatggg
     7681 tctcattagc cttagagtta attggttttg cagcctttac cgggttcgac catttatttt
     7741 tttgtaaaaa gctaactaca atttctgaat tcttcaaatc gttatctatc aggtagagct
     7801 cttcgccatt gtaagatagg aatgaggtgg tgaggtagct gctaccaaga ttccgggtaa
     7861 tatcctttgg ggttgaccat ttatccttat ccatgaagga gatgaacacc ttgttacccg
     7921 aggtggatcg gctggtgaag gccatcgttt ttccatcgcc cgaaagaacg gcatcgaagt
     7981 tcgactggtc gttgtttaca ccttcctcaa gtcggtttag gtttagccca attgggctgt
     8041 ccatcatagc cttggctgtt tcacagctct ttatgttttg ttcaacctgc tgcaaaacat
     8101 acgcatcatt tgggttgacg tactttttat aggcttcaaa ctgagctttg gctttatcaa
     8161 aattattttg atgcatgtag gagatgccta ggtggtagag aacttcaggg ggtgagttgg
     8221 cctccttaag cgaacgggcg tcgtacttgt cggatacctt ttttgcagct tcctcaaggt
     8281 aggagagggc ttcagccttc ctgtcatctg ttttcagcag gcagtatcct actttaaaca
     8341 tcaggttaca gctctcgggg tattttttta gggctttaag gtaggtatcg gccgctctgg
     8401 agtagttacc atcggccatg taaaattccg catcagtttc aagggtgaac aggagattcc
     8461 aatcctgtga aaaggatttc gttgggaaga atatcaaaat taaaaagtag aggtaggaaa
     8521 gatgcctaag gttatgcata ggaattatgt taaatcaaaa tgttaatttt tctacgaaaa
     8581 tatacacaat gggttgattc atgatcgccg tttttgcttt aagctgctag caaatttgac
     8641 caacatcatt ttttccttga tgtagatagc atggttatat gacaaaagcg caaggatgaa
     8701 tttgtgatga aaggtacaaa acttttttat tccatcttca attttgtggg gagttgtttt
     8761 aaatttcacc taatctatta ccaatgttgg ccaaaactcc ttaccccgta agggcttaag
     8821 ctgtaaaatt ttggtttgaa gttgtgtatt tagcttaaag aaacttcaac tttacctctt
     8881 ttaaaaattt ttcctgaagg aggttggcta tacgcttaac cacggttcgt ctgatttcca
     8941 actcaaccgg taggttgggg tcctggtggg caatattgat gcaggtgcct attacgaagt
     9001 caattatatc actttcgaga atcagcttaa ccagctgctc ggccggtccg ttgccaaggg
     9061 taacggtttc gttgtaggaa tccagaatgg tgttcacctt gctcagggtg agtattccct
     9121 cggtaaccag gtcagcacca tccatctggc tggttggggg taagtcgggg tcggtaaact
     9181 ccatgctgtc aacgacttca cgtttgagct cacgggcaat aatatcaact gtagtggcgc
     9241 cacagattac ctttttaccc tcgtagctat tgaaaatact tgccagcatg gagtcatttt
     9301 ccttttcaaa aggtgggccg gtgcaaatta gtagcttacg gggatgccgg aagtatatgg
     9361 ttgcacaggt agtatcatcc tttgggtggt agccgtcatt aacgtaagcc atgtttacca
     9421 ccttggcact tagcttacgg gccgatatgt cgggttcgtt ttttaccatt ttttctacaa
     9481 actctcttgc cccatcactc cagccaaatg gataatcggg tgttcccatg cccgattggc
     9541 ttacgccatc ggtccatagt attatcctgt cctccttttg cggggtaaag ctgcagtacc
     9601 tcaactcctt tccggcattt tgctcgctgt tgagtattat gcactgccat tcgggtatta
     9661 gcggttcggt gccccgcatg ataaagcatt ccgggttatc atactcaaga atccgggtat
     9721 cgccgttgtg ctcaatgtcc acaatggaaa aggtggagta gctcattttt ctttcggagc
     9781 aaacggggag ggtgttcatg attatttcag caatagtgtt gacttcctta tgttcgcggg
     9841 tgaagttgaa tgccattgtt gaggtgagtg tggccagcac gttggccttc acgccatgac
     9901 ccattccgtc cgaaagcact attatggttc gctcttcatc ctgaagacgt ttggagtaaa
     9961 acacatcacc gcaaattcgc tcaccatcgt gattcttctg ctggcagttg acttctataa
    10021 aaaatttctc ggacatgggg tgaaggctgt ttgcttaaac cattattttg ttttgctgtt
    10081 ttctgctttg gcaaccccct cgttgtaggt ttgaataatc gagttgagca tttgctcaat
    10141 ttctgaggca ccttcgccta gtaaaaagcc aatcttttga accattgcca ggttcctgtc
    10201 gatggcttcg gttacccgct taacaacctc ttctttctgc acggccggag catacatatc
    10261 cctgataaca gcaccaacaa tgctgttggg cctgatggtg aatacggaga tgttcagcag
    10321 cttatcgcgg tagtgaacat cacggtttaa tatatcgcta ttattttgaa gtacgtaggt
    10381 aaacaggttg tgaatattgt agggtaccag gctttttagg tctgcaccaa caagtccggg
    10441 aacaatctcg tttatttcta gggcgtcatc gccaagcaaa tcaataaagc tttggttgct
    10501 ctgtatgatt tttagcttct tatcaataag cacaactgca gcaggtagct tcttaagcat
    10561 ggtggtaacc gtttcggaag cctcacgagc tgcttcctgc tctattcgcg cctttttttc
    10621 cgattccctc aaagcctcct gggttttagc aagcttctcg ttagttgaac gaagggattt
    10681 aatatactcc tgcctgtttt ttagggtgaa gttcaagcac atgtccgtat gggcaagccc
    10741 cgatgccact gcagaggcaa agtcgtagca tgaggcatat ccacaagctt cacaacctgt
    10801 ttcctttcca acaaattcct tgtcaattac ctttagcacc tcctcaagtt tttgctgtga
    10861 ggggagtggc agcctttggt catcaggggt gaaatccctt gtatagctat tcttttcatt
    10921 tcgggatatg ttttcctccc attttttacg gttaaacgtt agcaatcttt tgttggcata
    10981 gttaaccacc attgttcggc gaatgaattt ttttccaccc ttcgatgtac ccggtcccat
    11041 aaggcagccc tcgcagtaga aaaggttaaa atgccggtta atcatttcgg cgctggtttc
    11101 gaattgctcc agggcttgaa gtgtgttgtt gggcccgtta gcgctgataa ctgttccggt
    11161 tagcaagtct tcgctgatgt caacggcttg cagtatgcct ctgccaatgg ggtaaagcga
    11221 tcccttgtag ccaattggtt cgtcaaactc ggagtactcc aggttgctct cgtttatgtt
    11281 aaattcctcg aatagctggc ggagctccac aaaggtaagc acctcgtcaa tctggctttt
    11341 cccgttgtat agctttgctt cctccttgct ttgtatgcag gggcctatat gaacaatccg
    11401 gacattactt ccatactggt tgtgtacagc ctgggcggta gcaatgatag gcgaaatgat
    11461 aggggcaagg ttgttgacca tttcgggatg gtacttttca accattgata caaccaccgg
    11521 gcacatgctg gtgatgaagt actttccctt aaagtcatcg aacagctttt tatactccct
    11581 ggctacaagg tcaacaccaa aagtaacttc gcaaacgtaa gtaaacccta aggtttttat
    11641 catctgaaca aactttcggt agtcagttat atccacaaat tcacccgaaa tgcttggcga
    11701 gcaaatagca gcaaccggtt cgttgccctt aagtagctcc agaacgtttt tcttggagga
    11761 acggtagctt accgcattca ccgggcaaat attgaggcag cttccacaac ccacacacct
    11821 gttttggagg attcgggcaa agtcctgacc ggctttaact tcgatggcct ttaccgggca
    11881 aacccgcaca caagcataac agtttatgca cgtttcctca cttatttcga agagctgcga
    11941 catttgtttt aatcgtttgg attagtactc gctcaactcg ctatttagga tatccagaat
    12001 attgtactcg tcaacgcctg taaaaatttt cccattaacg gttactgtag gcccttcagc
    12061 gcatctgcca aagcaatgcc ccccgtgaaa gtaaaccttt tcccttaggt gatgatcgtc
    12121 tatgtatttc tgtatggtct ttagggttgc tttatttccc ctcgcaaaac aggagctacc
    12181 caagcatatc acgatttcgt tttttttctc catcttattg tctggctggg ctttataata
    12241 cctttcccat gttctcctgc caatccttta aattgtttcc tctgagcgtt taatgattaa
    12301 ccctcttgct ggcttactta aaaaaacttc tgttaattca atcacaaatt taagtaaagt
    12361 tagattatct aaatttcttt tattaatttg ttcgggaatt aacaggtgaa aataccaact
    12421 taaatatctg ttatacagga aaatagttaa ttgttattga gttaacaatt tttaaaaatt
    12481 tcttgatggg taatgaaaag aagcattgaa gctatacttg aacagtatga ccatgccgga
    12541 atggacagct taatccccat tttgcaggaa attcagaata aaaccggatg cttaaatgag
    12601 gagtcaattg tcatggttgg taagcatttg ggcataccca cttccaaagt ttatggtttg
    12661 gctacatttt acaaccagtt taaatttgag ccaaagggta agttccatat attggtatgc
    12721 aatggcactt cgtgcaggct gaacggctca aaatggatca ttgatttcat tgagaagaat
    12781 ttaaaaatta aaaacggaga gactagtcgt gatggcacat ttagcctgga ggtaatttcc
    12841 tgcatgggag cttgtgagtc gggaccgctt atttcgatta atgaagagta ctattgcaac
    12901 ctcacaccgg ctaagcttgg tgaagttctt gccgggttag ccgagtcgga gtagtgccat
    12961 ttggatattc aacattttca tccacaatgg gttaagttgt gatgctatat ttgatatact
    13021 tggagttttg aacatagctg ttattgaaag gttatgaacc acagttacac tttagccgat
    13081 aaaacttttt tttgtgaaca ggtgctaccc ttgcttcatg ccgagctaaa tagtgatacc
    13141 caaaaacact tatcaacata caggcacgaa agggtaaaaa aaacagtcat atacattggc
    13201 attacaactt catcggttgt ggctggcgcc ctgaaaactc tgaaagcaat agagggctat
    13261 cttgaagaaa attcagtaga tgccataatt gtacccgttg gcagctatgg cctttgctcc
    13321 ttcgagccaa ttgttgaggt tcagctgccg gggaaaaagc gggtggcctt cagaaacgta
    13381 acatacgata gagttgaatt cctgctcgat ggtgcattta acagctttgt tcaacccgat
    13441 acggcacttt ttcagcacaa ttccgatatt cttgaaccgt ggcccgatat tccagttaag
    13501 gatgatttcc ctttttttgc aaaacaggat aggctacttc ttagaaatat tggaattata
    13561 aatcccgaaa atctactgga gtacatctca ttcgggggtt accgggcact tgcctatacc
    13621 ctgcggaatc aaacatccga ttccgtttgc cgtattgttg agctaagcgg gttgagggga
    13681 cgtggaggcg gaggctatcc taccggattg aaatggcgta aaaccctttc ggttgctgct
    13741 accgaaagga ttatgatttg taatgctgac gaaagcgatc ccggcgcttt tatggatagg
    13801 ctcttagcgg aaagcgatcc ctttcgtatc attgaaggtt taacaataag cgcttatgcc
    13861 attggtgcaa acaaagcaat tatctacatt agaaaccgta acaagctggc cgttgaccgg
    13921 cttaccctgg ccatggagca agcttacgat tttggtttgc ttgggtacaa cattttagat
    13981 agcggctata gcctcgatat ttcaattttt caaggccctg gagcttacgt ttgcggggag
    14041 gaaactgctc taattcaaag cctcgaaggg aaacgtggta cgccttctat taaacctccc
    14101 taccctaacg aggtggggtt aaatggtaag cctaccgtgg ttaacaatgt cgaaaccctt
    14161 gccagtatcc cagatattct ccttaatgga gctgactggt ttatgaaaat agggacggcc
    14221 acatcaaaag gaactaaaat tttctctgta aatggtaagg cttcccttac ctgtgtggtg
    14281 gaaatatcca tgggcaggcc actctcagcc attgtagagc tggcaggagg ggttaagccc
    14341 gactgcacgt taaaggcaat ccatctggga ggcccatctg gggttagcgt tacccctgac
    14401 gagctatacc tccctgttga ctttgatgag ctgaaagtcc acggcatacc acttggttcg
    14461 gggggagtcc agatactcga ccagtcggtt tgcatggttg acctgaccaa gtttttcatg
    14521 cacttcatcc ggaacgagag ctgcggaaag tgtatccctt gcagggaggg ctctagccgt
    14581 atgtaccaaa ttttgaatga gatttcccgt cgtcccgatt cctccgacaa gcaccacacc
    14641 ctcgagcgct ttaagggtgt aattcagctc gaaacgcttg ctgaggttat gcacgatacc
    14701 tccctttgcg ggctaggtca aaccgcttcc aagccattgt taaaagcttt aaaggcattt
    14761 agagacgagt ttgaggagca catttttgac cgtagctgta gggccggagt ttgccgcgac
    14821 cttagaactt tttacattga tactgagaaa tgcacgggct gtgccgcctg cgccaaaaag
    14881 tgtccaacta acgccattat tggaactcct cgctctccct actttattgt agaggataaa
    14941 tgcacgggtt gcggcagctg catggaggtt tgcaagtttg catcggtttt ctatagataa
    15001 cctgcatttt tggttaaaat tatgaaatat tcccaactaa gagcgaaacc taaaggtgcg
    15061 aggaatgaag aagcgttatt ggaagctaag ccttttgttt tatttttttg atacagattg
    15121 attactgact tgtttgaaat aatttaacac atgaatttca acattgaggt taacaaccgt
    15181 caaattctag ttcatcgagg agaaaccatt ttaaacgccc taagggggaa tgggattaaa
    15241 attccaaccc tttgcaacat gaaggagttg tcgcctaccg gtgcctgccg tctatgcgtt
    15301 gttgaggttg agggacgtga aaggctggtt acgtcatgtt cgcaacttgt tgaggagggc
    15361 atgcggatta aaacccattc atcaagagtg gtaaaagcac gtaaaacgtt ggttgaactg
    15421 ctacttgcca accacccgga cgattgcctt tactgcgaaa gaaacggacg ctgcgagcta
    15481 caatcccttg ccgttgagct gcatgttcgc gaacggcatg cccaaaacaa aaccttctcc
    15541 caaaagctcg acctatcaag ccacagcatc attcgtgatc catccaagtg tgtgctttgt
    15601 gggcgttgtg ttcgaatttg caatgagctt cagtctgtat ccacgtttga atttgttaag
    15661 cggggttgca atatgtacat aggcacagcc atgtcgcgcg atttaacctt ctcaaactgc
    15721 attgcttgtg ggcagtgtgt aatgtcatgt cctaccggtg cgttaagcga acgctcaaac
    15781 gttgatgagg ttatagatgc ccttaacaac cccaacctta ttccggttgt tcaggtggcc
    15841 ccatctattt ccgtatcgct tgctagcgag ctggggctca aaccaggtaa agatttgaat
    15901 ggggtgctaa acgccattct acgtaaaatt ggctttagat acgttttcga cagtgctgct
    15961 gcggccgata ttgcggtgat ggaaacagct actgaactct tgaagcggag ggaaaatgga
    16021 gagaagcatc cgcttatatc gtcatgctgt ccggcatggg taaagtttat ggagcagtac
    16081 taccccgaac acctttcatg gctatccact actaaatcac cccagcaggt gatgggggcg
    16141 cttattaaat cgtattttgc accatcctac ggcattaatc cggcaggtat tttctcggtt
    16201 tcagtaatgc cctgcctggc taagaaaatt gaagcccgac gggaggagat gactaccaag
    16261 ggtgtttcgg atgttgatac ggttatttcc acccgtgagc ttgtgcagct ggtagaactg
    16321 tacggaattg acgtacagaa tgttgaatcc caacaacccg attccccctt cgatattcgt
    16381 agttcggcag gtaagctttt cggcgtttca ggaggatcgt ccgaagctct tgcccgcacc
    16441 ctgcactaca tgaatacgga cacggagctg gacaacttta aaattgcaga actacgttca
    16501 tcttccggca ttagagagtt taaacttacc acaggtaaac aggaatttgt gtttgtagtg
    16561 ctgagcggca tgaaatccat tcatagcttt atggctgagc ttatcaatgg aaatgttagg
    16621 gccgattatg ttgaggtaat ggcctgtcct ggaggatgtg ttaatggtgg cgggcagcct
    16681 tttgcaatgg accctaagga gataaggcaa agaaccaaat caatatacga actcgatgag
    16741 gccgatgtgg taaaatcatc ccacaaaaac cccgttgtga aaagtgttta cgatgatttt
    16801 ttgaaagaac catgcggtga acttgcccaa aagtttcttc atacttccta ttacaagcgc
    16861 gacgtgctac tatgacaagc tagctaagtt gggaggtctg gccgatgcta attcgaaacg
    16921 tgtaggtaaa aaagataatt aatagtaaag tccatgcagg aggaaaatcg caagctggta
    16981 tatacaataa aggaactttg tagggtttgc tatacctgtg tgagggagtg tccggctaaa
    17041 gctatcagaa taatcaatgg tcaggctgag gtgattgccg aacgctgtat aggatgcggg
    17101 aattgtgtga aggtgtgcag tcaggatgca aaagtttacc tgcaaacacg aatggaggtc
    17161 agggatttac tgcagtcgga ggataaggta gccgcccttg tagctccaag ttttccggct
    17221 gagttcagcg aaattgatga ttaccggaag tttgtaggca tgctccggca gcttggattt
    17281 tcctatgttt tcgaggtagc ctttggtgcc gatttggttg ctcgaaggta taaggagttg
    17341 cttgaagacc gaagcggcaa aggctacatc tcctcggatt gcccggcaat tgtaacctac
    17401 atacgatact atcatcccga tttaaccgaa tacctggccc cggtggtatc acccatgatt
    17461 gctataagcc gcgtagttcg aaaaaagttg gggaatgggg ttaagttggt atttattggc
    17521 ccatgtattg ccaaaaaagc ggaaaccgat gaggttgatg aggcgctaac ttttcgggag
    17581 ctacgaggta tgctggaata cgcaggtgta acccccgaag cagcaacccc ctcggaattt
    17641 gacctacccc gtgccggacg gggtgctata ttcccggtaa gccgggggtt aattcaaacg
    17701 gtacgggtaa acgaggattt ggtagaaggt aacgttacgg tggctgaagg tcgtatggga
    17761 tttcaggagg ctattaagga gtgggaaatt ggaaacctta aggggcagaa cctcgagcta
    17821 ctctgctgcg atggctgcat aatgggtccc ggtatgtcgc ctggtggtaa acgatttgaa
    17881 cgtaaaaccc agattagcag gtatgcccgg caaaagcttt cggagcttga gcatgatgag
    17941 tggctttata gcatgaacga atttagggat attgatgttt ccagatcttt ccagcgtgat
    18001 gataggcgaa tgcctatacc atcagacgat cagataaaca gggtgctggc cagtatggga
    18061 aaattctcgc cccgcgacca gctaaattgc ggagcttgtg gatatgaaac ctgcgttgag
    18121 catgccattg ccataattga tgggttagcc gaggtggaaa tgtgcttacc ctacgccatc
    18181 gaagaacttc acatttctgt tgacaatctt gccatttcaa acgaaaagtt ggcaaaaatg
    18241 cagcaagcgt tgaagcaatc agaaaaactt gcccatatgg ggcagctttc ggctggaatt
    18301 gcccatgagc taaacaaccc tttaggtgta gtgctgatgt acagcaatat tcttatggac
    18361 gaatgcaagc ccgacgatcc tctacgcaag gatttggagc tgatagccac acaggccgag
    18421 cgatgtaaaa aaattgtggg tgggctgctc aactttgccc gtaaaaatca ggttaaagta
    18481 gaaagggttg atatacgaaa acttgctgca gaaagcgtaa cctctgtagt tatccctaag
    18541 accatttcaa ctcaggtagt atgcaaggct aacaataccg aggcctatct cgattacgaa
    18601 caaatgatgc aggttctaac caatctaaat aagaatgctg tagatgccat gccaaacgga
    18661 ggggtgctta ccgttgaggt ggatgagaat gaaagcgatg taatttttag ggtgagcgac
    18721 accggaacgg ggatacctga ggagaatatg gataaacttt ttaccccttt cttcaccacc
    18781 aagggaactg gcaaaggaac cggtttgggg ctggccacta cctatggaat tgttaagatg
    18841 cacagcggga aaatagaggt ggactccaac accgatcccg ccaaaggccc cactggtacc
    18901 acttttacca ttgtgcttcc ccggaaacct actttaggtt aaatgttcaa ttttttaaat
    18961 tgatggatca tgcctactcc tgcaaagaaa accattttaa ttgttgacga tgacgttgac
    19021 tacctgtacc agctgaaatt tcaggttgaa aaaatggggt ttgaaacggt aactgccgaa
    19081 agccagcacg aagcggaaac gctgcttaac accattaaac ccgacctggc cattctcgac
    19141 ctaatgatgg agagcgagga tagtggtttt atattaagct acaagctaaa aaggaagtac
    19201 cccgatgtgc ccattatcat tgcaactgct gtttcggccg aaactgggat gacattcgga
    19261 atcaacaccg aggaggaacg gaagtggatt aaggccgatc tttacctgga aaagggcatt
    19321 cgccccgacc aacttcacag ggagattgtt aaactgttaa aactttagca cgatatggag
    19381 gttgttgacg ttttggtggt tgacgatgag ccaggaattc gctctggtgt ctcacgaata
    19441 cttaaaaact acaccattaa ctatccattt ttggaggacg acattagctt taatgtggtt
    19501 gaagccgcca caggtgagga ggctattgag attatccagc gcaagccgcc tgcaatagtt
    19561 cttcttgaca ataaacttcc gggaattcaa ggaattgatg tgctccagtt catcagtgct
    19621 catgaaccca acatcctggt tatgatgatt acttcctatg cttcgttgga gctggcggtt
    19681 aaagccacca acattggcgc tcacgatttt gtccccaaac cctttactcc gcaggaactt
    19741 cgctcgtcaa tcgagaatat taccaagcac tacttcctcc gtcaaatgac ccgaaaactc
    19801 catcgcgaag gcaaacaggt gaggtttgag tttttaacca tgctgtcgca tgagctcaaa
    19861 gccccactga atgccattga gggttacctt agaatgatgc aggagaaaca gcttggcgaa
    19921 agtttggatg actaccagga aattatcgac cgctcgcttt cccgtattca gtccatgcgc
    19981 aacctgatta tggacctgct tgaccttaca cgggtagaat cgggtaagcg ggaaagagac
    20041 ttgcaggata ttgaccttac cctggtagct cggctggtgg ttgaaaccat gatgcctatg
    20101 gccatacagc gcgatgttac tcttaccctt gaatctgatg agccggtagt tgttaaagcc
    20161 gatgccgacg acatggaaat tcttcttaac aaccttatat ccaacgcaat aaagtataac
    20221 gtagaggggg gatgggtgaa atgtaaattc agcaaaaccc ccacctatgc aacctgcagt
    20281 gtttctgact cgggtgttgg tattagcgct gatgacattc caaagctatt ccacgagttt
    20341 acccgcatca aaaaccccct tagccggaac gttagcggaa gtgggctagg gctttccatt
    20401 gtaaaaaaaa ttgccgatca gtacggcgcc gagataaagg tggaaagcac gttgggaaaa
    20461 ggctcaacct ttaccctcca atttccggta tctgccgaaa accctgccta gctagcactg
    20521 acctttgtta aacatatatc tttcttttac ttcataacct aaagaaaagc gtaacatttt
    20581 taaaaagata tttagttttg tgcatgattc tacattttcg ctattttcaa tacgagttga
    20641 agttggcgaa gaaaatagtt tactagtcca aagctatata aatttacctt taaaacatgc
    20701 atagccttat gttcataggc aggctaagga ttgtgatgaa tataaagctg aacttactaa
    20761 gtcaatttaa attttattaa tagaatgaag cttcccgaga aaaccgttga aaggctgagt
    20821 gaatatcgtc gtaccctgct aagttgtata gctaaaggga aaacgcacat ttactcccac
    20881 gagttggccg gaattcataa catcacagct gttcaggtaa ggcgcgattt aatgcttatc
    20941 ggctactcca gcataaagaa aaaggggtac gacacccagg agttggccga tgtaataggc
    21001 gcccttattg accacgagaa aggcttaaat gttgccgtag taggaatggg caacatgggg
    21061 cgtgctatca cttcatactt caacggtaag cggccaaagc tgaatattgt tgctgcattc
    21121 gacgttgatc cgaataaggt taaccgtgtt atagctggtg ttaactgctt tcacatgaag
    21181 gatttacttg aaatttccaa acggtatgag atttcaattg ctatcattac ttccccgccc
    21241 gactcggcaa cccaggtggc tgaacagctg gtttctgccg ggattaaagg aatccttaac
    21301 tttaccacca ttccattaaa tgtccccgat acagtttatc ttgaagaatt tgatatgatt
    21361 acttcattgg aaaaggttgc ctatttcgtt aaaactaagg atgagtagcc ggtaaaactt
    21421 caaccttaaa cgttgtctcc taaatgcagg tacactatgg ttttgataat gttggctata
    21481 ttaagaatcc tgtggttaca acgggctctt ttgatggcgt gcatataggc cataaagcca
    21541 ttcttaagag aataaacgat attgccagag gtattgacgg tgaatcggtg cttattactt
    21601 tttatccgca cccccgcaag gttctgtacc ccgaaaatgc tggtaagaat ctggtcttca
    21661 tactttcaca aaaggaaaaa attgaacttt taagcaaaac aggtcttgat cacctgatta
    21721 tcgtcaggtt taccctggag ttttcccata tctcgtcggt tgactttata cagcattttt
    21781 taatagcaaa gcttcacgcc cggttcgtag ttgtcgggtt taaccaccat tttggccata
    21841 accgcgaggg agattttgaa gagctgaaac gccttggcct gcagtacaac tttggagtag
    21901 aggaaattcc agagcaggat attcagcagg aaaccgttag ctccactgca atccgtaagg
    21961 ctttgctcga gggtaaaatt caacgggcca acgcctacct cgaccatcac tttattatta
    22021 tgggtacttt gggcaaaggt gctcatattt tccagagagc aggattcccc acccttaccg
    22081 ttcaaattga ggaagcagga aagctgatac cccacgaggg tatttacgct gtcactctcg
    22141 aatggaatag cacccattac cgcgccatgg ccattatttg gctcgacaat tacggcagta
    22201 atgaacccgt tttagggcca ctcaacgttg acgttcatat aatggagttt gataatatcc
    22261 tttccgagga tgatgcaaag ttgttttttc aaaaacaact tgccgatcat gttgacatag
    22321 ctgatgagga acgcctggct agtcagcttt cggcagcttg cagctccttg gatgagctca
    22381 ttttttaaag gagcagc
//
LOCUS       DAOPHX010000027        22265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_2453_length_22265_cov_6.375371, whole genome shotgun sequence.
ACCESSION   DAOPHX010000027 DAOPHX010000000
VERSION     DAOPHX010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 22265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22265
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2453_length_22265_cov_6.375371"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1652)
                     /locus_tag="PK502_03420"
     CDS             complement(147..1652)
                     /locus_tag="PK502_03420"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HPG99898.1"
                     /translation="MNDSEILMNPNELVRFLKKPSAEFTKNDIIRFCEAKNIEMVNFR
                     YVADDGRLKTLNFAISSKEYLDTILSAGERVDGSSLFSFIGAGSSDLYVIPRYRTAFV
                     NPFSEVPAIDILCSFYDSEGKPLESSPENVLRKAHNLFKSETGCTFKALAELEYYVIS
                     ERDDFFPGTDQKGYHSSAPFAKWEDLRKEALICITRAGGMVKYGHAEVGCFMTEDTLY
                     EQHEIEFLPMPVEDAVDQLVIAKWMVRMLGYRYGVDISFAPKITVGKAGSGLHFHLMV
                     EKDGKNLVFENGRISNTSKKMIAGILDAADAITAFGNTIPTSYLRLVPHQEAPTNICW
                     GERNRSVVIRIPLGWVGTEQMVTHANPADTSAQPAGLNRQTFEYRVADCSADPYLTVA
                     ALVVASLNGLRMKDALQLADKLYVDVNIFSPEYKEKLAALKQLPASCWESAEALEAKR
                     HIFEADGIFPKGMIDSRINNLKSFQDKGLSEKIYGKTEEIRQLVERFIHIG"
     gene            complement(1944..4580)
                     /locus_tag="PK502_03425"
     CDS             complement(1944..4580)
                     /locus_tag="PK502_03425"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPG99899.1"
                     /translation="MDSVRELSAKYSPAGIEDKWYRYWLEKGFFSSTPDEREPYCIVI
                     PPPNVTGVLHMGHMLNNTLQDVLIRRARLMGKNACWVPGTDHASIATEAKVVAKLKAD
                     GIEKSQLSREEFLKHAWEWKEKHGGIILEQLKKLGASCDWSRTRFTMDETLSASVIHV
                     FVDLFNKGYIYRGVRMVNWDPQAKTAVSDEEVVYREVKGKLYFVRYRIEGEDSFVTIA
                     TTRPETILGDTALCVHPDDPRYTHLKGKRVIVPLVNRSVPIIYDDYVDREFGTGALKI
                     TPAHDVNDYLIGQKHNLDSIDIFNDDGTISSAAKLYVGLDRFEVRRRIVDDLESSGNL
                     ERVDDYVNSVGFSERTDAIIEPKLSMQWFLRMKELAEPALKAVVEGDIQLYPPKFVNT
                     YRHWMENVKDWCLSRQLWWGHRIPAWYLPNGGFVVAHNVEEAVELARKAAGNSGLVAD
                     DLRQDEDVLDTWFSSWLWPISVFNGILEPNNPDIRYYYPTNDLVTAPEILFFWVARMI
                     MAGYEYMGDKPFRNVYLTGIVRDHLHRKMSKQLGNSPDPLKLIEKYGADGVRVGMLLC
                     SPAGNDLLFDENLTEQGRNFCNKIWNAFRLVKGWQVDESAVQPQSAIAAIEWFNSLLN
                     KSIEQADDDFAKYRISEALMNLYKLFWDDFASWYLEIIKPAYGKPVDAVTYNATVSIF
                     EKLLIMLHPFMPFITEELWQHIRQRGDGESIMVQQLPHSTGFNQDILIGFDRVKEVVV
                     GVRNIRQENNISPKQPLQLSIRNAAKLDAGLEEIISKLASINSLTYIDQKIEGAATFM
                     VGTTEIYIPLGGLINTEEEREKILSELEYTKGFLEKVMKKLNNERFTSNAPKAVVELE
                     HKKQADALAKIEALQERLKSLG"
     gene            4821..5195
                     /locus_tag="PK502_03430"
     CDS             4821..5195
                     /locus_tag="PK502_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HPG99900.1"
                     /translation="MKKKLMVILLLTTALAASAQDFVGSAPEWVNNPPKSVRKFYGVG
                     VGTSTTPEMAEEKAKMDAKTAIATQAGAVKVENIKSDKGVTQRKIVNATLSEVKMVKK
                     ACMKDGERYTVWVLMEMKGKRK"
     gene            complement(5238..5654)
                     /locus_tag="PK502_03435"
     CDS             complement(5238..5654)
                     /locus_tag="PK502_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPG99901.1"
                     /translation="MKSYRKELWFETRNRRELINITGEVEKCLAESGISEGLALVNAM
                     HITSSVFINDDESGLHHDFEIWLEKLAPEKPYAQYRHNGFEDNADAHLKRTIMGREVV
                     VAITNGKFDFGPWEQIFYGEFDGKRRKRVLVKMIGE"
     gene            complement(5685..7433)
                     /locus_tag="PK502_03440"
     CDS             complement(5685..7433)
                     /locus_tag="PK502_03440"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPG99902.1"
                     /translation="MNADSSDSQVMERAKQWLSPVFDMETREQVQQLISGNHDELVES
                     FYRDLEFGTGGLRGIMGVGTNRMNIYTVGIATQGLANYLKKQLPGIPEVRAAIAFDSR
                     NNSIKFAQIAAKVLSANGVRVYLFDSLRPTPELSFAIRTLKCHCGIVVTASHNPKEYN
                     GYKVYWEDGAQVVPPHDSGIIAEVQAIKNISEVNFNGNESLISTIGPEMDEIYSNTLS
                     SLSLSPKIIKKHKEIVIVYTPIHGTGVKLVPKTLRKFGFTNIVNVPEQDVVDGNFPTV
                     SSPNPEEPSAMRLALEKAKEVNADVVMATDPDADRVGLGVRTPEGKYTLLNGNQAASI
                     LIYYVLQKWHEANRIKGREFIVKTIVTTDLLAEIARKYGVECYEVLTGFKYIAEKIRQ
                     LEGEKKFIAGGEESYGYLCGDFVRDKDAVISCALFAEATAWAKEQGKSLYEVLAGIYR
                     EFGLYSEKQLSITLKGKEGLEEISRMMVGFRKNPPLQIDGLPVITIHDYLSQETKDVP
                     SGSRRPLQLPKSDVLQFLTADGTKVSVRPSGTEPKIKFYIAIRGDFNPNAGIDEQLAL
                     LDERISRIAAELKITR"
     gene            complement(7451..8323)
                     /gene="rfbD"
                     /locus_tag="PK502_03445"
     CDS             complement(7451..8323)
                     /gene="rfbD"
                     /locus_tag="PK502_03445"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPG99903.1"
                     /translation="MAASPSRVLVTGANGQLGTALRKRATKPANHLVATFTDFNELDI
                     TSDQQVDNFFQQNHFDYLVNCAAYTAVDRAEDEPEKAFLINGYAPGLLAEACKSHGTK
                     LFHISTDYVFDGMSNSPYHESSPVNPLSVYGKSKLMGENKVLESGVGMVIRTSWLYST
                     TGNNFVKTILRNASSKAQLNVVFDQVGTPTLADDLAAVLLQIIEKGDSSFRPEIFHYS
                     NEGVCSWYDFAYEIVAIAGLPCRINPIETNDYPTRAIRPRYSVLSKEKIRNLLPIEIP
                     YWRDSLKTFLKEML"
     gene            complement(8307..8876)
                     /gene="rfbC"
                     /locus_tag="PK502_03450"
     CDS             complement(8307..8876)
                     /gene="rfbC"
                     /locus_tag="PK502_03450"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HPG99904.1"
                     /translation="MLTVEKTSIPDLLILKPNIFEDSRGYFFESFNAKLFDDFAITSN
                     FVQDNESFSKYGVIRGLHYQLSPYSQTKLVRVVYGKILDIALDIRRNSPTFGRYVAIE
                     ISAENKAQFYIPKGFAHGFSVLSDSAIVCYKCDAYYSKEAERGIRFNDPGLKIDWGIP
                     DGSMVVSEKDLKLPFFSEADINFNYGSIT"
     gene            complement(8916..9791)
                     /gene="rfbA"
                     /locus_tag="PK502_03455"
     CDS             complement(8916..9791)
                     /gene="rfbA"
                     /locus_tag="PK502_03455"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HPG99905.1"
                     /translation="MKGIILAGGAGTRLYPITKSISKQIIPIYDKPMIYYPLSVLMLA
                     QIRDILIISTPKDLHLYQDLLGDGAQLGISISYAGQPSPDGLAQAFIIGENFIGSDSV
                     CLVLGDNIFYGGNLTEMLLESAKLQDGAIVFGYNVKDPQRYGVVGFDSDGKVNSLEEK
                     PTNPKSNYAVTGLYFYSNDVVAKAKGLKPSKRGELEITDLNRLYLEEGRLKVKLMGRG
                     MAWLDTGTHESLLQASIFIQTIEERQGLKIACLEEIAYRNGYINLEQLRKLATPLENN
                     QYGEYLKRLASGNFS"
     gene            complement(9796..10737)
                     /locus_tag="PK502_03460"
     CDS             complement(9796..10737)
                     /locus_tag="PK502_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPG99906.1"
                     /translation="MKKILVTGGAGFIGSHLCERLLNEGNEVVCLDNYFSGKKENVVA
                     LMSNPYFELVRHDVTMPFFAEVDEIYNLACPASPIYYQYNPIKTVKTSVMGAINMLGL
                     AKRVKAKILQASTSEVYGDPLVHPQPETYWGNVNPIGARSSYDEGKRCAETLFVNYHS
                     QNGVKIKIARIFNTYGPRMSVNDGRVISNFIVQALRNEDITIYGDGKQTRSFMYVDDL
                     VDALIRLMDSPDEFTGPVNLGNPCEYSMLELASKVIELTKSRSKLVYFPLPEDDPLQR
                     KPDISLAKKFLGGWEPKVMLEDGLKKTIDSFEHILIR"
     gene            complement(10949..11359)
                     /locus_tag="PK502_03465"
     CDS             complement(10949..11359)
                     /locus_tag="PK502_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99907.1"
                     /translation="MSRAISILITFLLLFTIESCFDNENTVRYTDVIQMEAGPVPDTM
                     VVNETYSIQFRMGVPNSCWHSLALNQEEFNDSTYRFWATAVYENHGENCAQVVVTKDT
                     VIAFKPTLAKPHILVFFNDPASDPRVDTVVVTPN"
     gene            complement(11420..12253)
                     /locus_tag="PK502_03470"
     CDS             complement(11420..12253)
                     /locus_tag="PK502_03470"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPG99908.1"
                     /translation="MKHKPIIHVVFTLLLLVVSEATLSQSVNPCGIVNRSFASGEKLV
                     FEISYTWLFVWTDVGMAELTVSAEKRFGRDLLHIKASGKTHTFYNWFFEVHDLYETWV
                     DPVTVEPVYFNRDIYEGGFTKENEYFYDWNKNQVAIRVRRKKGPNRYDTLSVSRCTYD
                     VISAVYAARNLDFSGIKPGKTFPVEVLMDEEVYHIGYRFLGREEKRVSGLGRFRCLKF
                     QIDVVAGDIFSGDQKLYLWVTDDANKLPVAVETPIQVGSIKARLNTYEGLRHPLSSKI
                     P"
     gene            complement(12421..14529)
                     /gene="recG"
                     /locus_tag="PK502_03475"
     CDS             complement(12421..14529)
                     /gene="recG"
                     /locus_tag="PK502_03475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPG99909.1"
                     /translation="MADLILNDIKFLPGVGPKRAELLQKELNISTFGDLLYFFPFRHI
                     DRSKIYKISEINEDLPYIQIVGRILRIEEVGVGRNRRLTAYLSDGSGSVELVWFRGVS
                     WMAKWLKPNIEYLVFGKANVFGGHFSIAHPEMEEYKTGQEKIMGTLQPVYSTTEKLKS
                     NRITSKTILGLQQTLIQAVNGKIEETLPPYLIEQQKLMTLPEALRNIHFPQSVLLLRK
                     AEHRLKFEELFFIQLDILRKQTIRQSKINGHLFSRVGEMFNRFYSERLPFPLTNAQKR
                     VIREIRRDMGSGKQMNRLLQGDVGSGKTIVALMSMLIAVDNGFQACLMAPTEILANQH
                     FDSITSLLGDLPVTVGLLTGSTRQSERNTLHKSLEAGELQIIIGTHALIEDVVKFKNL
                     GFVVIDEQQRFGVAQRAKLWEKNNNPPHVLVMTATPIPRTLAMTVYGDLDVSVIDELP
                     PGRKPVKTLYYTDLKRNVVFGLMRQQLKEGRQVYVVYPLIKESEKMENLKDLQDGYES
                     IVRAFPPPEYVTVVVHGQMKPEEKESSMDIFVKGKANIMVATTVIEVGVNVPNATVMV
                     IESAERFGLSQLHQLRGRVGRGADESYCILVSSLKISSESRKRLQIMVNTSNGFEIAE
                     MDLKLRGPGDIEGTLQSGIPFDLKIANLSTDGNLLQHARAVARDVLNDDPLLEHPRNA
                     LLSQRLDELANSRSNFGRIS"
     gene            complement(14845..16854)
                     /locus_tag="PK502_03480"
     CDS             complement(14845..16854)
                     /locus_tag="PK502_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HPG99910.1"
                     /translation="MTLIFDLQVIDRLFAKFAPADFTTLDRFEEILKQYWGYNIFRSI
                     QRDIIESVYNGNDTLALMPTGGGKSITFQVPALALEGTCLVITPLIALMKDQVENLKK
                     RDIRAVAVHSGLTRHEVDVALDNCAYGDVKFLYLSPERTTTELFRERLRKIKVSMVAV
                     DESHCISQWGYDFRPSYLRIAELREIISEPVPFLALTATATPEVVEDIQQKLKFRNGK
                     VFSMSFARENLVYLVRNVEDKPKHLLKITHGIPGSGIVYVRSRSKTREIAQMLQKEGI
                     SADFYHAGLTMEVRNAKQNDWQVGKTRVIVATNAFGMGIDKPDVRFVVHVDLPDSPEA
                     YFQEAGRAGRDQKLAYAVLLFNNADSLKIEQRVESNFPPIEEIKRTYQALGSYLNVAI
                     GAGKGETYDFNLMDFCSAYKLSSVRAYSSLKILEREGYLELTDELDNPSRIMFLAGKQ
                     DLYKFQVAHAELDGFIKAILRNFTGVFSQYVRIDEVYLARLAGITVDKVIENLKTLGR
                     YKIISYIPKKRTPLLVLVEERLDDKNLRIEPSSYRLLRSRFEDRVKAMLSYANNQSKC
                     RSQMLSEYFGQKDSYRCGKCDVCTSRNELDMSKYEFDMILDGLKRRLQAGPCSEEELI
                     DAIKRNPDRVARVIKYLMDTGKIFHNEKGMLAWHHRKEGGFEVEE"
     gene            17011..18492
                     /gene="guaB"
                     /locus_tag="PK502_03485"
     CDS             17011..18492
                     /gene="guaB"
                     /locus_tag="PK502_03485"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPG99911.1"
                     /translation="MSFQSEKVIAEGLTFDDVLLVPAYSEVLPRNVNLSTKFSRNIDL
                     MAPIVSAAMDTVTESTLAIAIAREGGIGVIHKNMSIEAQARQVREVKRAENGMIFEPI
                     TISPDATIGDALTLMRENHIGGIPVIDSLGFLIGIVTNRDLRFHEDVSTPIRQVMTSD
                     NLITTSKSIDLENATEILKKYKIEKLPVVDENNKLVGLLTYKDITKVKDNPNACKDKM
                     GRLRVAAGVGVTRDTLERVRALCEANVDAIVIDTAHGHSKGVVDMLKLVKREFPNIDV
                     VVGNIATAEAAKMLAGAGADAVKVGIGPGSICTTRIIAGVGVPQLNAIWDVSHALEGS
                     GIPVIADGGIRYSGDIVKALAAGADSIMTGSLFAGVEESPGETIIYNGRKFKSYRGMG
                     SIDAMQQGSKDRYFQDVEDDIKKLVPEGIVARVPYKGTLSEVFYQLVGGLRAGMGYCG
                     TTSIEMLKKARFVKITHAGIIESHPHDVAITREAPNYSRDTDY"
     gene            18679..20664
                     /locus_tag="PK502_03490"
     CDS             18679..20664
                     /locus_tag="PK502_03490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPG99912.1"
                     /translation="MNKIIRNTLAGFMVITCGIAAVAQPTEQKILLTINNEPVTAGEF
                     QGLYLKSTSIENAKTTREVLEDYLNLFINYKLKVKAAKDNGIDTTAAFKNEYQGYVEQ
                     LAQSYLIDNQVLNNLIEEAYNRMQYEVSASHILISIPENASPSDTLKCYQKAMEARSR
                     ILKGETFGSVAVAMSNDPSVSRNSGFLGYFTAFQMVYPFESAAYSTPVDSISMPVRTR
                     FGYHIIKVHDKRPARGQVKVAHIMIRVPPNPSPQAAEDARLKILGIYQRLTAGADFAQ
                     LAKDESQDPSTSKSGGELPWIRPGQVIPEFEAVAFSLQHDGEISQPFQSSAGWHIVKR
                     LAKKELGNREELIPEIRAMLMRDSRGMLPRTAFIENLKKEYSFKQDTSKLKLLIPLLD
                     SSLYKGTWKSPNLKSNSTLISFNGNELKLSDFCNFIEQNQGLIGKHSIPAFLMNAYTE
                     WVNLTLTSYEKSKLPDKYPEFAQLTREYYEGMLLFEISNREVWQKSSDSLGIANFYNE
                     HQDSYKWGKRIHFAVYTLTDPKIKKAFVSGIEKRSKKGLSPLDYANRFNSPAKQVATV
                     VEKSGDPTLPEVADYNSWPKKFSVRDTQNGEVVVVEVLDTTIGDVKPLTDCKGEVISD
                     YQNSLEREWLEKLKKHYTVIVNRSVFDEISAWQNNNK"
     gene            20673..21530
                     /locus_tag="PK502_03495"
     CDS             20673..21530
                     /locus_tag="PK502_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99913.1"
                     /translation="MKSFRLVTFSLVGLTVVLASCFNSGKKPMPLARVYNSYLYPSDI
                     ANLIVGNVSKEDSALIVDAYIDKWIQTQLMLKIAENNLSDSQKNVTRQLEDYRTSLLI
                     FKYEQAYLNQKLDTVIKMADLKAYYDDNKDNFVLNETIVKALYIKLRREAPQIKKIRE
                     LYLSNSDEDIKQLDNIAYQAATKYDYFNDKWIPLSFILRELPFPIANPQREVFEKRYV
                     EMEDGDFVYLVRFMDAIPSGSTAPFDYITDDLKQIVLNARKQKLISELEQRIYSKGQD
                     DENFQVYKR"
     gene            21569..>22265
                     /locus_tag="PK502_03500"
     CDS             21569..>22265
                     /locus_tag="PK502_03500"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPG99914.1"
                     /translation="MKPARLAITALLLQLFIVNAYSQEAQVIDQVVAVVGGEHILLSD
                     IEQELLRQKMEGSPTGENNRCNVLEQILIQKLLLTQAKIDSLKTNESSVEMEIDRRLK
                     YFTSQLGSEKALESYFNKPIYEIKNDLREAIGEQLLTQQMRQKIVDKVKITPAEVKSF
                     YKNIPTDSLPTVPEQYELQQIMIYPPSTEEAKLQVREKLLEIRNRILQGERFSTLAVA
                     YSEDRASAIRGGEL"
BASE COUNT         6185 a         5279 c         4892 g         5909 t
ORIGIN      
        1 gaatcttgaa tcttgaatct tgaatccgcc ggtaggcgga caggtttgaa ccctgagcta
       61 gtcgaagggt gaatccgccg gtaggcggac aagttcttga attattttat cctttatcct
      121 tgtctgagcc tgtcctctgc ctttttctat ccaatgtgga taaatcgctc caccagctgc
      181 cttatttctt cggttttacc ataaatcttt tcgctaaggc ccttatcctg gaatgatttc
      241 aaattgttaa ttcgggaatc tatcatccct ttggggaaaa ttccatcggc ttcaaagata
      301 tgccttttag cctcgagcgc ttcagccgat tcccaacagg aagccggaag ctgctttaag
      361 gcggcaagct tctccttgta ttcaggacta aaaatattta catctacgta gagcttatcg
      421 gcaagctgca gggcatcttt catacgtagg ccattgagcg atgcaaccac cagcgcagca
      481 acggttaggt agggatcggc tgaacaatcg gccaccctgt actcaaaggt ttgcctgttc
      541 aatcctgctg gctgtgcgct tgtatcggca ggatttgcat gggtaaccat ctgttcggtg
      601 ccaacccagc caagtggaat acggataacc accgaacggt tacgctcacc ccagcatata
      661 tttgttggcg cttcctgatg gggtactagc cgtaaatagg aagtaggaat tgtgttccca
      721 aaagcggtta tggcgtcggc tgcgtctaga attcccgcaa tcattttttt tgaagtattg
      781 cttatccttc cattttcaaa caccagattt ttcccatcct tttcgaccat caggtgaaag
      841 tggaggccgc tcccagcctt ccccacagtg atttttgggg caaaacttat atcaacgccg
      901 taccggtaac caagcatgcg caccatccac ttggcaatta caagctggtc aacggcatcc
      961 tcaaccggca tgggcagaaa ttctatctcg tgctgctcat aaagtgtgtc ctccgtcatg
     1021 aagcatccca cctcggcgtg gccatacttc accataccac cggcacgagt aatgcagatt
     1081 aaagcctcct ttcggagatc ctcccacttc gcaaaaggag cagatgagtg gtagcccttt
     1141 tgatctgttc cggggaaaaa atcatcgcgc tcgctaatta cgtagtactc caactcggca
     1201 agagccttaa aggtacaacc tgtctcagac ttaaaaagat tatgagcttt ccggagcaca
     1261 ttttcaggtg aactttcgag tggtttgccc tcgctatcgt agaaagagca tagaatatca
     1321 attgctggaa cttcagagaa aggatttaca aaggctgtcc ggtagcgggg aataacgtat
     1381 agatcgctac taccagcacc gatgaacgaa aacaagctgc taccatcaac cctttcgcca
     1441 gccgaaagaa tagtatccag atactccttt gaggagatgg caaagttcag ggtttttaac
     1501 ctgccatcgt cggccacata gcgaaagttg accatctcaa tattcttagc ctcacaaaag
     1561 cggatgatgt cattcttggt aaactcagct gaaggttttt taagaaaccg aaccagctca
     1621 ttggggttca tcaaaatttc agaatcgttc attttaaccg gtcttagatt aatattttac
     1681 aaaattacga ttttacagat tacagattta aaggatttcg gatgtttcat aacataattt
     1741 caaatccgca ggctcaaaat ggaaaaaccg taaccaaact ttacatttta aagggcagat
     1801 agaagtaaat tcaaaaaaag aggtgagcat tttggatgaa tattccaaaa tgatattcca
     1861 ctcaaagctg taaatcatta cagcttgtta caggcagaga atgattgcgc tatgaattag
     1921 taatattcta aagttcgagg gcactacccc aatgacttca agcgttcctg tagagcttca
     1981 atctttgcca gggcatcggc ttgttttttg tgttccaact caacaacggc tttaggcgca
     2041 ttgcttgtaa aacgttcgtt gttaagcttt ttcattactt tttcgagaaa tcctttggtg
     2101 tactcgagct cggaaagtat tttttcccgt tcctcctcgg tgttaattaa ccctcctagc
     2161 gggatgtaaa tctccgtagt gcccaccatg aacgtagctg caccctcaat tttctggtca
     2221 atgtaggtca acgagtttat cgaggccagc ttagaaatga tctcctcaag accagcatcg
     2281 agcttagcgg catttcgtat gctcagctgt aggggttgct tgggtgaaat gttgttttcc
     2341 tggcgtatat tcctaacgcc cacgacaacc tcttttaccc tatcgaaacc gattagaata
     2401 tcctggttaa atcctgttga atggggtaat tgttgaacca taatgctttc tccatcccct
     2461 cgctggcgta tgtgttgcca aagctcctcg gtaataaatg gcatgaatgg atgaagcata
     2521 atcagcaact tctcgaatat gcttactgtt gcgttatagg taacggcatc tacgggttta
     2581 ccgtatgcag gtttaataat ttcgaggtac catgacgcaa aatcatccca gaaaagcttg
     2641 tagaggttca tgagtgcttc cgaaatgcgg tactttgcaa agtcatcatc agcctgctca
     2701 attgacttgt tcagcaggga gttaaaccat tcaatggcag caatagccga ctgcggttga
     2761 actgcagatt cgtcaacctg ccagccctta accaaccgga aggcgttcca tattttgttg
     2821 cagaagttac gaccctgctc agtcaggttt tcgtcgaaaa gcaggtcgtt tccggcaggt
     2881 gagcataaaa gcattcccac cctaacccca tcggccccgt acttctcgat caactttagc
     2941 ggatcgggtg aatttccgag ctgcttgctc attttgcgat gcaggtgatc acgaacaatc
     3001 ccggtgaggt aaacattccg gaaaggttta tcgcccatgt actcgtaacc ggccataatc
     3061 attcgggcaa cccagaagaa aagtatttcg ggtgcggtaa ccaggtcgtt ggttgggtag
     3121 tagtacctga tgtcggggtt gtttggctca agaataccat tgaaaaccga gattggccag
     3181 agccacgagc taaaccatgt atccagcaca tcctcgtcct gtctaagatc gtcggccact
     3241 agaccggaat taccggctgc tttccgtgcc agctctaccg cttcctcaac attatgagca
     3301 accacaaagc ccccattggg aaggtaccat gcaggaattc tatgccccca ccaaagctgg
     3361 cgtgaaagac accaatcctt tacattttcc atccagtgac ggtaggtgtt gacaaactta
     3421 ggaggataga gctgaatatc gccctccaca acagccttga gggctggttc agccaactcc
     3481 ttcatgcgaa ggaaccactg catcgaaagc ttaggctcaa ttatggcatc ggttcgctcg
     3541 ctaaagccaa cgctgtttac atagtcatct acccgttcaa ggtttcccga gctttctaaa
     3601 tcatcaacaa ttctccttcg cacctcaaat ctatccaaac ccacgtatag ttttgctgcg
     3661 gaactaattg tgccgtcatc gttgaatatg tcaatagaat ccaggttatg tttttgacca
     3721 atcaggtagt cgtttacatc gtgagcggga gtaatcttga gagcgccggt gccaaactcc
     3781 ctgtcaacat aatcatcgta aataattgga acgctacggt ttaccagcgg aacaatcact
     3841 cttttacctt tcagatgggt gtatcggggg tcatcggggt gaacacaaag ggctgtatca
     3901 cccagaatgg tttcgggtct tgtagttgca atagtaacaa acgaatcttc accctcaatc
     3961 ctgtagcgga cgaagtaaag ctttcccttc acttcgcggt aaaccacctc ctcgtctgaa
     4021 accgccgttt ttgcctgcgg gtcccagttc accatgcgaa cgccgcggta tatgtatccc
     4081 ttattgaaaa ggtcaacaaa aacatggata acactagccg aaagggtttc gtccatggta
     4141 aagcgggtgc gggaccagtc gcatgaggcc cccaactttt tcagctgctc aagaataatc
     4201 cccccgtgtt tttccttcca ctcccacgca tgtttaagga actcctcacg gcttagctgt
     4261 gatttttcaa ttccatcggc tttcagctta gccacaactt ttgcctcggt ggcaattgag
     4321 gcatggtcgg tacccggcac ccagcaggca ttcttaccca tcaacctcgc ccgccggatg
     4381 agaacatcct gaagggtgtt gttgagcatg tgccccatgt gcaaaacacc cgtaacattt
     4441 ggtggaggaa ttacaatgca gtagggttca cgctcatcgg gcgttgagct aaagaaaccc
     4501 ttttctaacc agtacctgta ccacttatcc tcaattccgg ccggactgta cttggccgac
     4561 agttctctta cgctatccat aggctagatc aaaaaaaaat cgaacggtaa aaatagctaa
     4621 attttgaaag ctcaacgcat ggagcatcct acctacccga gcaattaaaa ttttccaagg
     4681 attggggaaa ctagagcctg gcacaatgca atggcacaaa atgtgatgta taaaaatcgg
     4741 aaataggttt gagtaaattt taaaaaaaat tagtggttgt acaactagta aatggcttat
     4801 aacctttaat attaaccgga atgaaaaaga aactaatggt tatactccta ctaacgacag
     4861 ccctagctgc ctcagcacag gattttgtag gttcagcgcc tgaatgggtt aataacccac
     4921 caaaatcggt aagaaaattc tatggtgttg gtgttggaac atctactacg ccggaaatgg
     4981 ccgaggaaaa ggcaaaaatg gatgccaaaa ccgctatcgc cacacaagct ggtgcagtaa
     5041 aggttgaaaa tattaaatcg gataaggggg ttacccaaag aaaaattgtt aacgccaccc
     5101 tttcggaggt taaaatggta aaaaaggctt gcatgaagga tggtgagcgg tacaccgttt
     5161 gggttttgat ggaaatgaag ggaaaacgaa aatagcggct aaaggacact tcggaaatca
     5221 aagagctgta atctggatta ctccccaatc atcttaacca gcaccctttt tcgtcgttta
     5281 ccgtcaaact cgccgtaaaa aatttgctcc cagggtccaa aatcgaactt accattggtt
     5341 atggctacca ccacctcgcg tcccatgatg gttcgcttta aatgtgcatc ggcattatcc
     5401 tcaaaaccgt tgtgccggta ctgggcgtat ggtttttccg gagccagctt ctcaagccaa
     5461 atctcaaaat cgtgatgaag cccgctctca tcatcattta tgaatacgct ggaagtgatg
     5521 tgcatggcgt tgaccaacgc cagcccctcg gaaataccgc tctcagccag gcacttctcc
     5581 acttccccgg taatgttaat cagctcacgc ctgttgcgtg tttcaaacca taattccttt
     5641 ctgtacgact tcataaataa gatttaacct gcacataggc aaaattaccg ggtaatcttt
     5701 agctcggcag caatccgtga aattctttca tccaaaagcg caagctgctc gtcaatgcca
     5761 gcattgggat taaaatcgcc cctaatcgca atgtagaact ttattttggg ctcggtgccc
     5821 gaaggacgaa ccgaaacctt tgttccatcg gcagtaagga attgtagcac atccgatttg
     5881 ggaagttgta aaggcctacg cgaaccgctc gggacatctt tggtttcctg ggacaggtag
     5941 tcgtggatgg taataactgg caagccatca atttgcagcg gtgggttttt cctgaagcca
     6001 accatcattc tggatatttc ctccaacccc tccttgcctt taagggtgat ggaaagctgc
     6061 ttttcgctgt aaagtccgaa ttccctgtaa ataccagcaa gaacctcata gagcgatttc
     6121 ccctgttcct tagcccaggc agtggcctca gcaaacaagg cgcagctgat aacggcatcc
     6181 ttatcgcgaa caaaatcacc gcacaggtag ccgtagctct cctcgccacc ggcaataaac
     6241 tttttttcac cctccagctg ccttatcttt tcagcaatgt acttgaaacc ggtaagcacc
     6301 tcgtagcact ccactccgta ttttctggca atttcggcta gcaaatcggt tgtaacaatc
     6361 gtttttacta taaactccct gcccttaatt cggttagctt cgtgccattt ttgaagtacg
     6421 tagtaaataa gaattgatgc agcctgattg ccgttgagaa gggtgtactt gccttcagga
     6481 gttctaactc caagccctac cctgtcggca tcggggtcgg tagccatgac cacatcagca
     6541 tttacctcct tggctttttc aagtgccaac ctcatggccg atggttcctc ggggttgggt
     6601 gatgaaacag tgggaaagtt gccgtcaacc acatcctgct cgggaacatt cactatgttg
     6661 gtgaaaccaa acttacgaag ggttttggga accagcttca ccccggtgcc atgtattggg
     6721 gtataaacaa tgacgatttc cttatgtttt ttaatgattt tgggagagag cgaaagcgac
     6781 gaaagggtat tggagtaaat ctcatccatc tccggtccta tagtgctaat caggctttcg
     6841 ttgccgttga aattcacctc cgaaatgttc ttaattgcct gaacttctgc aatgattcct
     6901 gagtcgtgag gtggaacaac ctgagcgcca tcctcccagt aaaccttgta tccgttgtat
     6961 tccttggggt tatgcgaagc ggttacaaca atgccacaat ggcatttaag cgttcgaata
     7021 gcaaaactca gctcgggagt aggcctgagg gaatcgaaaa ggtaaacccg caccccattt
     7081 gcgctaagga ctttggcagc aatttgggca aattttatgc tgttgtttcg ggaatcaaag
     7141 gcaatggcag ccctaacctc aggaatacca ggaagttgct ttttcagata gtttgcaagc
     7201 ccttgcgtgg caatgcccac ggtgtatata ttcattcggt ttgtaccaac acccataatc
     7261 ccccttagtc ctcccgtacc aaattccaaa tctctgtaaa acgactcaac cagctcgtcg
     7321 tggttaccac taataagctg ctgcacttgc tccctcgttt ccatatcaaa aacagggctt
     7381 agccattgct ttgccctttc catcacttgg ctatccgatg agtcagcatt catggcattt
     7441 cattttaggg ttatagcatt tcttttagga aagttttaag gctatccctc cagtagggaa
     7501 tttcgattgg cagcaagtta cgaatttttt ctttactcaa cacgctatac ctgggtcgaa
     7561 tggcacgagt tgggtaatca tttgtttcga tgggatttat acgacaaggt aggccggcaa
     7621 ttgccacaat ttcataggcg aagtcatacc agctgcaaac accttcattc gagtaatgaa
     7681 atatttcggg acgaaaggag ctatcgccct tttcaataat ttggagcaat actgctgcca
     7741 aatcatcggc aagagttggg gtgccaacct ggtcgaaaac cacgttgagc tgtgcctttg
     7801 atgaggcatt cctgagtatg gttttaacaa agttgttccc cgtagtggag tataaccacg
     7861 aagtgcgaat taccatgcca actcccgatt ctaacacttt gttttcgccc atgagcttgc
     7921 ttttgccata aaccgaaagc ggattgaccg gggaagattc atggtagggc gagttggaca
     7981 ttccatcgaa aacgtagtcg gtggatatgt gaaagagttt ggttccatgg ctcttgcatg
     8041 cctcggccag caacccgggc gcataaccgt taatcagaaa tgccttttca ggctcatcct
     8101 cggcacgatc aaccgcagtg taggcagcac aatttacaag gtaatcaaaa tggttttgct
     8161 gaaagaaatt gtcaacctgt tggtcgctgg ttatatccag ctcgttaaag tctgtaaatg
     8221 tagctacgag atgattcgcc ggcttggttg cccgtttacg aagagcggtt ccaagctgcc
     8281 cgtttgcacc tgtaaccaaa accctgctag gtgatgctgc cataattaaa attaatatct
     8341 gcttcagaga aaaagggaag cttgaggtcc ttttccgaaa caaccatgct accatcggga
     8401 atgccccagt caatcttcaa acccggatcg ttgaagcgta ttcccctctc cgcttcttta
     8461 ctgtagtagg catcgcactt gtagcaaact atagctgaat cgctcagcac tgaaaaacca
     8521 tgtgcaaatc cttttggaat gtaaaactgt gccttgttct cagcagagat ttctatagct
     8581 acatacctgc caaaagtggg agaatttctc cttatatcca gagcaatatc caaaattttt
     8641 ccgtaaacca ctcttaccag ctttgtttgg gagtagggtg acagctggta gtgtaacccc
     8701 ctgatgacgc catacttcga aaacgactcg ttgtcctgaa caaaattaga agtaatggca
     8761 aaatcatcaa atagcttggc gttaaagctt tcaaaaaaat atcctctgga atcctcaaaa
     8821 atattgggtt taagtatcag tagatcgggt attgaggttt tttcgactgt gagcatcact
     8881 tatcagcttt cgttaaaaaa gaaacgggtt agcggttagg aaaaattacc agaagccagc
     8941 ctcttgaggt actcaccgta ctggttgttc tccagaggcg ttgccaattt ccggagttgc
     9001 tccaggttga tgtagccgtt gcgataagct atttcctcca gacaggcaat tttaagcccc
     9061 tggcgttcct caatggtctg aataaaaatg gaggcttgca gcaggctttc gtgggtaccg
     9121 gtgtcgagcc aggccatacc gcgtcccatc agcttaacct ttagcctgcc ctcttcgagg
     9181 taaagcctgt tcaggtcggt tatttccagc tcgccccgtt ttgaaggttt taaaccttta
     9241 gctttggcca ccacatcgtt gctgtagaag taaagccctg ttacggcgta gttcgacttt
     9301 gggttggtgg gtttttcctc caggctattc acttttccgt cgctatcgaa acccacaaca
     9361 ccatagcgct ggggatcctt aacgttgtag ccaaaaacaa tagctccatc ctgaagtttg
     9421 gcactttcga ggagcatttc agtaaggttt ccgccataga aaatattatc gcccaggacc
     9481 aggcaaacgc tgtcggagcc aataaagttt tcgccaatga tgaaagcctg tgcaagccca
     9541 tcgggtgagg gctgcccggc atagctgatt gaaattccca gctgagcacc atcgcccagc
     9601 aaatcctggt agaggtgcaa atccttggga gttgaaataa tcaggatatc cctaatctgg
     9661 gcaagcatca gcaccgacaa gggatagtaa atcatgggct tgtcgtaaat cggtattatc
     9721 tgcttggata tacttttggt tattgggtac aaacgagtac cggcaccgcc cgccaaaatt
     9781 attcccttca tccttctatc taattagtat atgctcaaac gaatcaattg tctttttaag
     9841 tccatcctcc agcataacct ttggttccca accgccaaga aattttttag ccaggctgat
     9901 gtccggttta cgctgaagag gatcatcttc aggcagtggg aagtaaacca gcttcgacct
     9961 gcttttggta agctcaatca ccttactggc cagctcaagc atggagtact cacagggatt
    10021 tcccaggtta acaggaccgg taaactcatc gggagaatcc atcagccgaa taagtgcgtc
    10081 aaccaaatcg tcaacataca tgaagctacg ggtttgcttc ccgtccccgt aaatggttat
    10141 atcctcattt ctgagagcct ggactataaa gttggagatt acccgcccat cgtttacgct
    10201 cattcgtggg ccgtaggtgt taaaaatacg ggcaatttta attttaaccc cgttctggga
    10261 gtggtagtta acaaaaagcg tttcggcaca ccttttcccc tcgtcgtagc tggagcgagc
    10321 gccgatagga ttcacattgc cccagtatgt ttccggctgg gggtgcacga gcggatcgcc
    10381 gtaaacttca ctggttgaag cctgaagaat ttttgccttt acccgtttag ccagcccaag
    10441 catgttgatg gcgcccatca cggatgtttt tactgtttta atagggttat actggtagta
    10501 aatgggggaa gcaggacagg caaggttgta aatttcatct acctcggcaa aaaagggcat
    10561 ggttacatcg tggcgaacca gctcaaagta tgggtttgac atcagggcaa ccacattttc
    10621 ctttttcccg gaaaagtagt tatcaaggca aaccacctcg tttccctcat tcagtaaacg
    10681 ttcacagagg tgtgacccaa taaatccggc acctccggta accagtattt tcttcatagg
    10741 taacggtatg tagtaacccg ggttaactaa aatgaaccat caaaacggta aaataataaa
    10801 tatgagtggt ttaacaaatt agtatgatgg caaaaccagc aacatccttc acaacaaaaa
    10861 gtaaatgtgc cactcaacag tttttgcagc tgaaaaaccc gcaagccaac ctatcacggc
    10921 agggcgtgta tgccagcatt ttttggggtt agttgggcgt aaccacaacc gtatcaactc
    10981 ttggatcact ggcgggatcg ttgaagaata ccaatatatg gggcttagcc aatgtgggtt
    11041 taaaggctat tacggtatcc ttggttacta caacctgagc acaattttcc ccatgatttt
    11101 cgtaaaccgc agtggcccaa aaccgatagg ttgaatcgtt aaactcttcc tggttgagag
    11161 cgagcgaatg ccagcaggaa tttggaacgc ccattctgaa ttgtatagaa taggtttcgt
    11221 taactaccat ggtatcggga acgggacctg cttccatctg aataacatca gtatatcgga
    11281 ctgtgttttc gttatcaaag catgattcta tggtaaaaag cagtaaaaat gtgattagta
    11341 tggaaatagc tcttgacatg gcatttattt tttgattatg ccgctaattt atggaaaata
    11401 gttgtcatac ccatacaact tagggaattt ttgatgataa cggatgcctt aatccctcgt
    11461 atgtgttaag cctggccttt atgcttccca cctggattgg agtttccacc gcaacaggca
    11521 gcttgtttgc atcatcggta acccacaggt agagcttttg gtcgcccgaa aagatgtcgc
    11581 cggctaccac gtcaatttga aatttaaggc acctgaaacg tccaagccct gaaacccgtt
    11641 tttcttcgcg tccaagaaac ctgtacccga tatggtaaac ctcctcatcc atcaaaactt
    11701 caacaggaaa tgttttaccc ggcttaatac ctgagaagtc caaatttcta gctgcataaa
    11761 cagccgaaat cacatcgtaa gtgcacctgc taacactcaa ggtatcgtat cggttaggcc
    11821 cttttttacg acgtaccctt atggctacct ggtttttgtt ccaatcatag aagtactcgt
    11881 tctccttggt gaatccgccc tcatatatgt cgcggttaaa gtaaaccggc tcaacagtaa
    11941 ccggatcgac ccatgtttcg taaaggtcgt ggacctcgaa aaaccagttg tagaatgtat
    12001 gcgttttccc cgaagcctta atgtgcagta aatctctgcc aaaacgtttc tcggcgctga
    12061 ctgtaagctc ggccattcct acatctgtcc atacaaaaag ccatgtatag cttatctcaa
    12121 agaccaactt ctcacccgat gcaaaagacc tgttgacaat tccacaggga ttgaccgatt
    12181 gcgatagggt agcctcgctg acaaccaaaa gcaaaagagt aaaaacaaca tgtatgatgg
    12241 gtttgtgttt cattacgatt cttattatca gcaatcaact attttattgt aagctagatt
    12301 atgaacatct agttacttca atactaaaaa gtatattgga aatgaaggtt cattacaccc
    12361 caaaaataat acgataaaaa ttactactaa gctaaaattt tcccgcgtaa cgattagctg
    12421 ctagctaatt cttccgaagt tactccgtga gttggcaagt tcatcaagcc gttgtgaaag
    12481 aagtgcattc cggggatgct ctaatagcgg gtcatcatta agtacatccc tggcaacagc
    12541 ccttgcatgc tgaagtagat ttccatcggt gctaagattg gctattttca ggtcaaaggg
    12601 aattccgctt tgaagagtcc cttcaatgtc gccgggcccg cgaagtttga ggtccatttc
    12661 ggcaatttca aaaccattgc tggtattaac cataatctgt agcctcttac gcgattcaga
    12721 agaaattttt aaggatgaga caagtatgca gtagctttcg tcggctcccc gccctaccct
    12781 accgcgcagc tggtgcagct gcgacaggcc aaacctctcg gcactctcaa ttaccataac
    12841 ggttgcattg ggaacattaa cccctacctc gataacagtt gttgccacca tgatgttggc
    12901 cttgccttta acaaaaatgt ccatggagga ttccttttcc tcgggtttca tctggccatg
    12961 aaccacaact gtaacgtatt cggggggcgg aaatgcccgt acgatgcttt catagccatc
    13021 ctgaaggtct ttcaggtttt ccattttttc cgattccttt atcagcgggt aaaccacgta
    13081 aacctgccgg ccctccttaa gctgctggcg catcaggcca aaaaccacat ttcgcttcaa
    13141 atcggtgtag tacagcgttt taacaggctt tcggccaggt ggcagctcat caatcacgct
    13201 aacatccaaa tcaccgtaaa ctgtcatggc caaggttctc ggaattgggg tggcagtcat
    13261 aaccagcaca tggggtggat tattgttttt ctcccatagc tttgcccttt gggcaacacc
    13321 aaaccgttgc tgctcatcaa tcaccacaaa accaaggttt ttgaacttta caacgtcctc
    13381 aataagcgca tgagtgccaa taattatctg cagttcacct gcctcaaggg atttatgaag
    13441 cgtgttgcgt tccgactggc gggtgctgcc cgtaagaagc ccaacagtaa cgggtaagtc
    13501 tcctaaaaga gaggtaatgg agtcaaagtg ctggttggct aaaatctcag taggtgccat
    13561 gaggcatgcc tgaaaaccat tatcgacagc aataagcatg gacatcaatg caactatggt
    13621 tttgccgcta ccaacatctc cttgtaaaag cctattcatt tgctttccac tgcccatatc
    13681 gcgacgaatt tcacgaataa cgcgtttctg agcattggtt aaagggaatg gcagcctttc
    13741 gctgtaaaaa cggttgaaca tttcaccaac tcgcgaaaac aaatgcccgt tgatcttgct
    13801 ttgccgtatg gtttgttttc taaggatatc gagctgtatg aagaaaagct cctcaaactt
    13861 aagccgatgt tcggctttcc tcaaaagtaa gacgctttgg ggaaaatgaa tgttcctgag
    13921 agcctcggga agtgtcatga gcttttgctg ctcaatcagg tagggaggta gggtttcctc
    13981 aatttttcca ttgacagctt gaataagggt ttgctgaagt cccagtatgg ttttggaggt
    14041 aatcctgttg ctttttagct tttcggtggt ggagtaaacg ggttgcaggg tccccataat
    14101 tttctcctga ccggttttgt actcctccat ttcggggtgg gcaatgctga aatgacctcc
    14161 aaatacatta gccttgccaa ataccaggta ctcgatgttg ggttttaacc actttgccat
    14221 ccatgaaaca ccccgaaacc ataccagctc tacacttcca cttccatcgc tcagataggc
    14281 tgtcagccgc ctgttgcgcc ccacaccaac ctcctcaatc cgaagtattc gaccaactat
    14341 ttggatgtag ggtaaatcct catttatctc cgagatctta taaatcttag accggtcaat
    14401 atgcctgaag ggaaagaaat agagcaaatc gccaaatgtg ctgatattca actccttttg
    14461 gagtagttcg gcgcgcttag gccccacacc cggtagaaat tttatatcat ttaaaatcaa
    14521 atcagccata tacgttaaat tccagagctt gtccgactac cggcggaact tgtccgacta
    14581 ccggcggaac ttgtccgact accggcggaa cttgtccgac tactggcgga ttccagatta
    14641 aagagggtaa aggataaaga ataaagtata atttgaaagc cgcctcccgg tggaacttgt
    14701 tcaaccgttg atggcttaaa agtattcttt atccaattaa cttttaacat acttactaaa
    14761 aacacttttc gtgtcaaaac taggtttttc acgcctaatt gattcttctt aactttaagt
    14821 tgaagccaat agtcgcttag gtgctcactc ctctacctcg aatccgcctt cctttcgatg
    14881 gtgccatgcc agcataccct tttcgttatg gaaaattttt ccggtatcca taaggtactt
    14941 gatcaccctg gctactctat caggattacg tttaattgca tcgataagct cctcctcgct
    15001 acatggccca gcttgcaagc gcctttttag cccatccaga atcatatcaa actcatactt
    15061 gctcatgtcc aactcgttgc gcgatgtgca tacatcgcat ttcccacagc ggtaggagtc
    15121 cttctggcca aagtattcgc taagcatttg gcttctgcac ttggattggt tatttgcata
    15181 agaaagcata gcctttaccc tatcctcaaa gcgtgaccta agcaacctgt atgagcttgg
    15241 ttcaatgcgc aaatttttat cgtcaagcct ctcctcaacg agtaccagca gcggagttct
    15301 tttcttggga atgtagctta tgattttata cctgccaagg gttttaaggt tttcgatcac
    15361 tttatcaaca gtaatacccg ccagccgggc aagataaacc tcgtcaattc ggacgtattg
    15421 ggagaaaact ccggtaaaat ttcggagtat cgccttaata aacccatcca gttctgcatg
    15481 agcaacctgg aacttgtaga gatcctgctt accggcaaga aacatgatgc ggctggggtt
    15541 atcaagctcg tcggtaagtt caaggtaccc ttcacgctca agaattttta gcgagctata
    15601 agccctaacg gagctaagct tataggctga gcaaaagtcc ataaggttga aatcgtaggt
    15661 ttcgcccttg ccggcaccaa tggcaacgtt caggtacgaa cctaatgcct ggtaggttcg
    15721 cttaatctcc tcaataggag gaaagtttga ctcaacacgc tgctcaattt tcagtgaatc
    15781 ggcattgttg aaaagtaaaa ccgcataggc caacttttga tctcggccgg cacgcccggc
    15841 ttcctggaag tatgcttcgg gcgaatcggg aagatcaaca tggactacaa accggacgtc
    15901 gggcttatca atacccatgc caaaagcatt ggtggcaaca ataaccctgg ttttgccaac
    15961 ttgccagtcg ttttgcttgg cattacgcac ctccatggtt aagcccgcat gataaaaatc
    16021 ggccgaaatg ccttcttttt gaagcatctg ggcaatttct cgggttttac tccggctacg
    16081 gacgtaaaca atgccacttc ccggaatgcc atgcgttatt ttgagcaggt gctttggctt
    16141 atcctccaca ttcctaacca ggtaaacaag attttccctg gcaaaactca tgctgaatac
    16201 cttgccattg cgaaacttca gcttttgctg aatatcctca accacctcgg gtgtagccgt
    16261 tgcagtgaga gcaagaaaag gaacaggctc agaaataatc tccctcaact cggcaatgcg
    16321 caggtatgaa ggacgaaaat cgtagcccca ctgcgaaatg cagtgcgact catctacggc
    16381 caccatgctc acctttattt ttctgagcct ttcgcggaat agctccgtgg ttgttctctc
    16441 cggtgaaagg taaagaaact tcacatctcc atatgcacag ttatcaagag ctacatcaac
    16501 ctcatgccgg gtaagtccag aatgcacggc aaccgcccta atatcccttt tcttcagatt
    16561 ttcaacctga tccttcatta aggcaataag cggagtaata accaggcagg ttccctccag
    16621 ggcaagagca ggaacttgaa aagtaataga tttaccacct ccggtgggca taagcgctag
    16681 tgtatcattt ccattgtaaa ccgattcaat aatgtcccgc tgaatcgaac ggaaaatgtt
    16741 atacccccag tactgcttta gtatttcctc aaacctatcc aaagttgtaa aatctgctgg
    16801 agcaaattta gcaaataacc ggtctataac ctgcaaatca aaaattaagg tcatttagct
    16861 taaatattaa ttgggctaac gggcttactt cgagcaacgg cgttgccatt ttaaaagttc
    16921 accatgggct gcatgcaggt tgatggtaat tggctatttg tcattttttt gtaacttgcc
    16981 gcatttttac aacctaacca aactatgaat atgtcgtttc aatcggaaaa agtaatagct
    17041 gaggggctta cattcgatga tgtgctttta gtaccggcct actccgaggt gcttccacgg
    17101 aatgtgaacc tttcaacaaa gttttccagg aatattgacc tcatggctcc tattgtttcg
    17161 gctgcaatgg atacagttac ggagtcaacc cttgccattg ctattgccag ggaaggggga
    17221 ataggtgtta tccataaaaa tatgagcatc gaagcacagg ccagacaggt gcgtgaggtt
    17281 aagcgcgcag aaaatggcat gattttcgaa cccataacta ttagcccaga cgctactatt
    17341 ggagacgcac taacgctcat gcgcgaaaac cacattggag gaatcccggt tattgacagc
    17401 ctgggatttt taataggtat tgtaaccaac agagatcttc gctttcacga agatgtaagc
    17461 actccaatcc ggcaggtgat gacctccgac aacctcataa ccacctcaaa atcaatagac
    17521 ctcgaaaatg caactgagat actcaaaaag tacaaaatag agaagttgcc cgttgttgat
    17581 gagaacaaca agcttgtagg actgcttacc tacaaggaca taaccaaggt taaggacaac
    17641 ccaaatgcct gcaaggataa aatgggaagg ttaagggtgg ctgctggggt tggtgtaacc
    17701 agagatacgc tggaacgcgt aagggcactc tgcgaagcaa atgtggacgc catagtgatt
    17761 gatacagccc acggacactc gaaaggtgtg gtggatatgc tcaagctggt aaagcgagaa
    17821 tttccaaaca ttgacgttgt tgttggaaat attgcaacgg ccgaggcagc caagatgcta
    17881 gccggtgcag gagccgatgc ggttaaggta ggtattggac caggttccat ttgcacaacc
    17941 cgtatcattg ccggcgtggg cgttccacag cttaatgcga tttgggacgt ctcgcacgcc
    18001 cttgagggaa gcggtattcc ggtgattgcc gatggtggta tccgctactc aggcgatatt
    18061 gttaaggcat tagcagctgg ggccgattcc ataatgaccg gctcgctctt tgccggcgtt
    18121 gaggaatctc caggcgaaac cattatttac aacgggcgta aattcaagtc gtaccgtggc
    18181 atgggctcaa ttgacgctat gcagcagggt tcaaaggaca ggtactttca ggatgttgag
    18241 gacgacataa aaaaactggt tcccgagggt atagttgccc gagttcccta caagggaacg
    18301 ctctctgaag tattctacca gctggtgggt ggattaaggg ctggaatggg ctactgtggt
    18361 acaaccagca ttgagatgct aaaaaaagcc cggtttgtca agattaccca cgccggtata
    18421 attgagagcc atccccacga tgttgccatt actcgtgagg ctcctaacta cagccgcgat
    18481 acggactact agtagcttgc acgttttatt attttgcccg cagccatgcc ccagcagaag
    18541 cattaaaaat caaaaataac tacgcaaagg ttgttggctg taccatcaat ttaaggaata
    18601 tggcgaaggt gtaaaatagc taagatgaaa acgaaactgc tgcaatttgt tggcagtaaa
    18661 ctttaaacaa gtattcgtat gaataaaatt attaggaata ctctggcggg tttcatggta
    18721 ataacctgcg gtatagctgc tgttgcccaa ccaaccgaac aaaaaatttt acttactata
    18781 aacaacgaac ctgttactgc aggagaattt cagggacttt accttaaaag tacatccata
    18841 gaaaacgcaa aaacaacccg cgaagttcta gaagattacc ttaacctctt cattaactat
    18901 aagttaaaag ttaaagcagc caaagacaac gggatagata caactgcagc ttttaaaaat
    18961 gagtaccagg gatatgtaga acagctggca cagtcatacc taattgacaa tcaggtatta
    19021 aacaacctta ttgaggaagc atacaaccgg atgcagtatg aggttagcgc cagccacata
    19081 ctcatatcaa ttcccgaaaa tgcttcaccc tccgacacgt taaagtgcta ccagaaggcc
    19141 atggaggctc gttcaagaat tctaaaggga gaaacatttg gctcggtagc agttgcaatg
    19201 agcaacgatc cctcggtttc gaggaacagt ggctttctag gctacttcac cgcatttcaa
    19261 atggtttacc ctttcgaatc agctgcctac tccacccctg ttgattccat ctcgatgccc
    19321 gtgagaaccc gctttggcta ccacatcatc aaggtgcacg acaagcgacc tgctcgcgga
    19381 caggtaaagg ttgcccatat catgatacga gttccaccca atccttcccc tcaagcagcc
    19441 gaggatgcca ggttaaaaat tctgggtatt taccaacgcc ttactgccgg tgccgatttt
    19501 gcccagctag ccaaagacga atcgcaagac cccagcacct caaaatcggg aggagagttg
    19561 ccatggatac gtcccggtca ggtaattcca gagtttgaag ctgttgcctt ctccctgcag
    19621 catgatgggg aaatatcgca acccttccaa agttcagctg gttggcacat tgtgaaaaga
    19681 cttgcaaaaa aggagctggg gaacagggaa gaacttattc ctgaaataag agctatgcta
    19741 atgcgcgatt cacgaggaat gcttccccga acagcattta tagagaatct caaaaaggag
    19801 tattccttta agcaggatac ttcaaagttg aagctactga ttccgttact ggattcatca
    19861 ctctacaagg gaacatggaa atcgcccaac ctcaaatcaa actctaccct tattagcttc
    19921 aatggaaacg agctaaaact ttctgacttt tgcaatttca ttgaacaaaa ccaagggtta
    19981 ataggcaagc attctattcc tgcctttctc atgaatgcct acaccgaatg ggtaaaccta
    20041 actcttacca gctacgagaa gtctaagcta cctgataagt accccgaatt tgcccagctt
    20101 acccgcgagt actacgaggg tatgctgctg ttcgaaattt ccaaccggga ggtgtggcag
    20161 aaaagttctg atagcctggg aatagcaaac ttttacaacg aacaccagga tagctacaag
    20221 tggggcaaac gcattcactt cgctgtttat actcttaccg atccgaaaat taaaaaggcg
    20281 tttgtcagcg gtatagaaaa acgttccaaa aagggtcttt cgcctctgga ttatgcgaat
    20341 cggttcaact cgccggctaa acaagtggcc accgtagtcg aaaaatcagg tgatcccacc
    20401 ttgcctgagg tcgcggacta caattcctgg cctaaaaagt tcagtgttag ggatacacaa
    20461 aacggcgagg tggttgttgt tgaggtgttg gacacaacaa ttggtgacgt aaaacctctg
    20521 accgactgca aaggagaggt gatatccgat taccaaaact cgcttgaaag ggaatggctc
    20581 gaaaagttga aaaaacacta caccgttata gttaatagga gtgtgttcga cgagatttct
    20641 gcatggcaaa acaacaataa atgagataaa acatgaaaag ttttcgttta gtaacttttt
    20701 cgctggttgg gttgacagtt gttttggcat cgtgcttcaa ctcaggtaaa aagcccatgc
    20761 ccctggcaag ggtttacaac agctaccttt acccttcgga cattgccaac ttaattgtag
    20821 gcaacgtatc gaaggaagac agcgccctga ttgtagatgc ctacattgat aaatggattc
    20881 aaacccagct tatgctgaaa attgccgaaa acaatctatc cgattctcaa aagaatgtta
    20941 cccgccagct ggaggactac cgtacttcac ttctaatttt caagtacgaa caggcctacc
    21001 tcaaccagaa gctggatacc gtaattaaaa tggccgatct gaaggcttac tacgatgaca
    21061 acaaggataa ttttgtgcta aacgagacaa ttgtcaaggc tttgtacatc aagctgcgca
    21121 gggaggctcc acaaataaag aaaataaggg agctctacct gtcaaacagt gacgaagata
    21181 ttaagcagct cgataacata gcctatcagg ctgcaacaaa gtacgactac tttaatgata
    21241 agtggattcc cctctcattc atccttcgag aacttccttt tccaatagca aatccgcaaa
    21301 gggaggtttt tgagaaaaga tatgttgaaa tggaggatgg cgacttcgtt tacctggtaa
    21361 ggtttatgga tgccatacct tccggctcaa cagccccttt cgattacatc acagacgatt
    21421 tgaagcaaat cgtgctgaac gcccgaaagc agaaacttat ctccgaactt gagcagcgga
    21481 tatacagtaa agggcaggat gatgaaaact ttcaagttta taaaaggtaa atgtcaagct
    21541 actcattaga aattaaacaa tactacaaat gaaaccagca cgattagcaa taacggcttt
    21601 gttacttcaa cttttcatag ttaacgccta ctcgcaggaa gcgcaagtaa tagatcaggt
    21661 tgtagcagta gtaggtggtg agcatatcct gctttccgat attgagcagg aacttcttcg
    21721 ccagaaaatg gagggttcac caaccggcga aaacaaccgc tgcaacgtgc tggagcaaat
    21781 tcttatccag aaattgctgt taacccaggc caaaattgac agcttaaaga caaatgagtc
    21841 gtcggtagaa atggagattg accgaaggtt gaagtacttt accagccagc tgggatcgga
    21901 gaaggcgctt gaaagctact tcaacaaacc catttacgaa attaagaacg atttgcgtga
    21961 agctatcggc gagcagctgc ttacccagca aatgcggcaa aaaattgttg ataaggtgaa
    22021 gataacaccc gctgaggtga aaagttttta caaaaacata ccaaccgaca gcctacccac
    22081 agttcccgag cagtatgaac tacaacaaat tatgatttac ccaccctcta ccgaggaggc
    22141 aaagctacag gtgagggaaa aactactgga aatcagaaac cgcatactcc agggcgagcg
    22201 cttttcaact ctggccgttg cctactctga ggatagggca tcggcaatca ggggcggaga
    22261 gctgg
//
LOCUS       DAOPHX010000028        22124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_2472_length_22124_cov_5.301781, whole genome shotgun sequence.
ACCESSION   DAOPHX010000028 DAOPHX010000000
VERSION     DAOPHX010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 22124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22124
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2472_length_22124_cov_5.301781"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..742
                     /locus_tag="PK502_03505"
     CDS             278..742
                     /locus_tag="PK502_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPG99915.1"
                     /translation="MEMKDRIRLLLDAEKINSAKFAEAIEVQRSAVSHILAGRNNPSF
                     DIIQRIAQKFPKINLEWLITGKGSMYKIPVQRSIFDTDDSINQPGKTAPTANLTESRT
                     ITNVNNRGEGGKILSGRYEGADSEDTSKELTVQKIVIFYSDRTFVEYRPSIS"
     gene            785..1840
                     /locus_tag="PK502_03510"
     CDS             785..1840
                     /locus_tag="PK502_03510"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HPG99916.1"
                     /translation="MYKSIFRKLLFIFPPETVHKLVVSLIKFFFKIPGARNAFRRIFS
                     NRNEACLEREVIGLKFSNPVGLAAGFDKNAEFFNEFSSFGFSFIEIGTVTPEPQPGNS
                     RPRLFRLLPDNALVNRMGLNNKGVNYVKSRLQHKENQGLIIGGNIGKNSSTPNSNAVS
                     DYIACFNELYSFVDYVAVNLSCPNVHNLSDLQNPDSVKLIIESLVNERAKFPSKKPIL
                     LKVSPDLSPEQLNQTIDAAEEAGVDGYIATNTTTGRNNLKTKKSELEKIGRGGLSGKP
                     LRDVSTEVIRFINQKLDGKKPIIGVGGIFTPMDALEKLEAGASLVQVYTGFIYEGPFI
                     VKRINKLLINKMFYTYI"
     gene            1847..2467
                     /gene="yedF"
                     /locus_tag="PK502_03515"
     CDS             1847..2467
                     /gene="yedF"
                     /locus_tag="PK502_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HPG99917.1"
                     /translation="MAKLVDCRGMVCPQPLVETRREIKKSNPGEAITVVVDNSTSLKN
                     ILRFLEDNGVECVVKQEAESSSITFNVPLQMTQPDKRAEEYCDVSPKPDVNSGFIVLL
                     NSATMGKGNDELGKILMKGFLSTLLELKATPTEIICYNSAVTLALKSSDTAATLQQIS
                     ERGIKITLCGTCVDFYNLRDDLAVGQISNMLYIAERLSSGVRIIQP"
     gene            2547..3671
                     /locus_tag="PK502_03520"
     CDS             2547..3671
                     /locus_tag="PK502_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPG99918.1"
                     /translation="MNLYFDNSSTSFPKPEGVSQSINEYFSHGGTYGRAAYNRVIEVS
                     RKVESTRSLLANLIGTSLAPNVVFTSNATHAINLFIRGMRYPKRRVIISPLEHNAVCR
                     PLQYLFQTAGVGVDIFPHHPDGRIDIQKAAKVNFASYDLGIINHVSNVNGVIQPIKEI
                     KRLMGNTPLLVDASQSAGRVEIRADEWKLDALALTGHKGIMGPTGTGALFIRNPDMAD
                     PLIFGGTGSNSESYLQPIDLPDRFEAGTPNIVGIYGLWGALSSGMGYSFKREHFVELL
                     HEISTFSSFKLLAADDTDFQSDVFSIKPLGMEVSELARILFEKFSVETRSGLHCAPLA
                     HRTLGTFPTGAVRFSLSKFHTDNDLDYLHSCLKKIDEGRS"
     gene            3655..3894
                     /locus_tag="PK502_03525"
     CDS             3655..3894
                     /locus_tag="PK502_03525"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HPG99919.1"
                     /translation="MREEANTGFTILIFSNIHQVMHAEKIIADKGYPYRIVPVPSNLS
                     SECGLCIRIESSYLDSILSDLQNIKVEIHTLNNEP"
     gene            3924..4961
                     /gene="selD"
                     /locus_tag="PK502_03530"
     CDS             3924..4961
                     /gene="selD"
                     /locus_tag="PK502_03530"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPG99920.1"
                     /translation="MMEEFDLLSTVEQGGCSSKIPAALLESLLPALPKLVDENILVDF
                     SSHDDAGVYRISDDFALILTTDFFPPVCSDPFEFGQISAANSISDVYAMGGEPFLALN
                     ISMFPSSRIPIDVYKSILEGAASKALEANVAIIGGHTIDDFPPKFGLAVVGRVDPKRL
                     VTNSGLRPGDKLILTKPLGTGITVAAKRLKMVTDESYRIAIQWMKTLNDRACSVMKKY
                     HVTGATDITGFGLLGHLLRMARSSRVNISIQAGTLPVLPGVTSLVNDGCIPGAAFRNL
                     EYVENDVTFTRVDYDLKMIMVDAQTSGGLLMGVQDEFANQTLQELTDAGLCYSRIIGE
                     VGEGNGKIFVS"
     gene            5151..6041
                     /locus_tag="PK502_03535"
     CDS             5151..6041
                     /locus_tag="PK502_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99921.1"
                     /translation="MKLTKDSWITFTIERLVEIPQAGYYYRLLHESGQKFLLPAQYYT
                     HYNLVVGSTVSCRVDKINCMGKVFLEPKHPYYEVNMVYNFDFVAMNNSFVKGVAELVV
                     RDIFNETYTLFLPAEVCSKISNMVPLRVLNIRKGRLQLQLAGATGSCKEFEENEIIDL
                     TIETVVEINGEKFFFLSHSSGCFALLKVKYYLHYDFEVGKQVSCQYIGIKPDGTLRVE
                     PPNPFYRVGDTYNFTVSSVGTESNADGETASYATVFDIYGMKCGFVVPAGSKVQPDQV
                     ILCKVVGYKKGRPMLMIVQK"
     gene            complement(6065..6397)
                     /locus_tag="PK502_03540"
     CDS             complement(6065..6397)
                     /locus_tag="PK502_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99922.1"
                     /translation="MAKKRVVIKFGDLSPELLELFKQKYPHGYQNHVIKVEKPGGDYF
                     FAVTLDSDDASYLVKVNVKIDSKIKDEEDEKEFFGGLSDDIGSENDAFPDDMNEEDEP
                     SEEMSDDL"
     gene            6600..7064
                     /locus_tag="PK502_03545"
     CDS             6600..7064
                     /locus_tag="PK502_03545"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPG99923.1"
                     /translation="MTCVVPILFGYNAMAQVQIYNPNADAIKDLNQAIERAGVQGKHV
                     LVQVGGNWCPWCVKLNKIFTTDSTIMKVLNANYVLVKVNYSKENKNEAVLEKLEFPQR
                     FGFPVLVILDDHGKRIHTQDSGLLESGDGYDTKKIVSFLKNWTKAAIGKPAN"
     gene            complement(7134..8444)
                     /locus_tag="PK502_03550"
     CDS             complement(7134..8444)
                     /locus_tag="PK502_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG99924.1"
                     /translation="MKRLTTVLLFSVLGMGMLNAQTGVQINFDQFGKKIEKSNATIQD
                     PKKNAKPDAWIARAELFMDVFDAQIYRAYPGMDMKTFSLLIGKPTEQTQEVVEGITVD
                     KFVMDRAEFYFANGSLDHWQVTKPVVENPLEVAYESLIKAIELDASKKKAKPIMEDLL
                     KLKNYLINEGSSCYSKKDYGCSYKCFSKVVEIGKNPLVNQKDTAIYYYAGLSAQLDGK
                     FEECIPYYEESIKLGFTSEGSVYFNIFDAYKSLGKADEGAKYLEDGFIKYPKNTNVLF
                     SLISYYIDKGEDPSKILVYIDQAIANEPNNPSLFFAKGTLHDRLGDTDEALTSYQKAI
                     EIDPNYFDAYYNIGALFFNVGVKYVEEANKIPAKEIEKYDELMSKANVEFKKSLPYME
                     NALKINPASKEVMDALKNIYFRFRNESPEMSAKYKEIIDKLNNM"
     gene            complement(8506..10998)
                     /gene="gyrA"
                     /locus_tag="PK502_03555"
     CDS             complement(8506..10998)
                     /gene="gyrA"
                     /locus_tag="PK502_03555"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPG99925.1"
                     /translation="MSETERIIRINIEEEMKSSYIDYSMSVIVSRALPDVRDGLKPVH
                     RRVLFGMSDLGLNAGNPYKKSARIVGEVLGKYHPHGDSSVYDAMVRMAQDWSMRYPLV
                     DGQGNFGSIDGDSPAAMRYTEARLAKIAEDVLADLDKQTVDFNLNFDDTLEEPSVLPT
                     KIPLLLVNGTSGIAVGMATNMPPHNLGEVVDAICATIDSPDITTDELLKYVKGPDFPT
                     GGVIYGTQGVKEAYRTGKGKIVVRSKIEIEQTESGRSKIIVTEIPYMVNKAEMIRRIA
                     DLINEKKIDGISYINDESDRDGMRVVIILKKEAVANVVVNNLFKHSQLQSSFAINNIA
                     LVDGRPKLLNLKDLIVHFIKHRHDVIIRRSRFELEEAEKRAHILEGLLIAIDHIDEVI
                     NLIRSAQSPAEAKEKLVERFGLSDIQSQAIIDMRLRSLTGLEREKVKEEYNELQKLIA
                     YLKQVLEDKALQMKIIKDELVEVKQKYGDARKTEIIPDAEEFNPEDFYADEEVVITIS
                     HMGYIKRTPLYEYKLQSRGGVGAKGSDTRDEDFIEHLYVATMHNTMLFFTENGKCFWL
                     KVYEIPEGTKSSKGRAIQNLINIENDDVVRAYINVKGLDDTDYLKNNYIVLCTKRGII
                     KKTPLEAYSRPRQNGVIAVTVKEGDQLIEACLTDGKSHILIAARNGKAIRFPEHLVRP
                     IGRTGSGVRAISISPDDEVVGMVCVEENASKDILVVSENGYGKRSRLEDYRITNRGGK
                     GVKTINITPKTGKLISIKAVDENNDLMIINRSGLTIRLKISELRVMGRNAQGVRLINL
                     KKNDSIAAIANVESSENEPDEQNLVQEGESEQ"
     gene            11207..13756
                     /locus_tag="PK502_03560"
     CDS             11207..13756
                     /locus_tag="PK502_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPG99926.1"
                     /translation="MNQKFSQKIRDVLSYSREEAIRLGSSAISSDHLFLGILRDGEGE
                     AINILLAEGVNLSELKKELDVHLLQIPSKAEEINDSSEIAIYKSAERALKIVILEARA
                     LKSESINTAHLLLAILKDDTNYVTQLLKEKDIDYERVRKNLTILNSDDKGDFGSGDED
                     EGDSGFFGNMGKSNQPSTGKSASDTPVLDNFGIDLTKAAEDGRLDPIVGREREIERIA
                     QILSRRKKNNPILIGEPGVGKSAIAEGLALRIVKKKVSRVLFNKRVITLDLASIVAGT
                     KYRGQFEERMKAILNELSRTPNIILFIDEIHTIVGAGGATGSLDAANMLKPALARGEL
                     QCIGATTLDEYREHIEKDGALERRFQKVMIEPTSPEETIEILNNIKDRYEEHHNVTYT
                     PQAIEACVKLTMRYISDRNLPDKAIDAMDEAGSRVHISNIRVPDTIVNLEKLIEETKK
                     EKIKAVKNQQFELAANLRDKEKKLIEQLEEEQSRWEKELAKHREIVDEEKVAEVVAMM
                     TGVPVQRIAQAEGARLLKMADELRNSVIGQDEAIQKVVKAIQRNRAGLKDPNKPIGTF
                     IFLGPTGVGKTQLAKVLAKYLFDTTDNLIRIDMSEYMEKFSVSRLVGAPPGYIGYEEG
                     GQLTEKVRRKPYSVVLLDEVEKAHPDVFHILLQVLDEGQLTDSLGRRVDFKNTIIIMT
                     SNIGSRELKDFGQGVGFTTKAKEQSSSSDYSRSIIEKALKKAFAPEFLNRIDDIVMFN
                     SLSRADIHKIIDIELSGLYERIRNLGFTIRISPAAKDFIAEKGYDVQYGARPLKRAIQ
                     KYLEDALAEVLIKSDTKPGCTISVGYNKKKDEISIKVLKGEEEVTSASEEE"
     gene            13832..14332
                     /gene="tpx"
                     /locus_tag="PK502_03565"
     CDS             13832..14332
                     /gene="tpx"
                     /locus_tag="PK502_03565"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HPG99927.1"
                     /translation="MNVTFGGKTVTLEGEQVKVGSTAPDFTALGTDLKPVKLSDFGKK
                     VKLISVFPSIDTEICSIQNHRFNNEASSFGDKVAFIAISNDLPFALKRFCGAEKITNL
                     TTLSDHKDVDFGKKFGFLIKELRLLTRGVIILDKDNKVQYVEIVPEIASEPNYTEAIA
                     RLKQLV"
     gene            complement(14452..15765)
                     /gene="rimO"
                     /locus_tag="PK502_03570"
     CDS             complement(14452..15765)
                     /gene="rimO"
                     /locus_tag="PK502_03570"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009229376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPG99928.1"
                     /translation="MQQPGKGKINVVTLGCSKNLVDSEVLMNQLKMGGWEVVHDSNDT
                     EAKVVIINTCGFIADAKEESVDTIMGFVDAKNKGIIDKVFVMGCLAQRYRNELQDEVP
                     EVDGFFGVSELPAVLHALNTPVFSGCLTTRLLTTPGHYAYLKISEGCSWGCSYCAIPL
                     IRGKHISRTVESLVEEATILSEKGVKELILIAQDSTYYGMDIYGERRIAQLIDRLSQI
                     KGIEWIRLHYAYPAHFPDDLIATIANNPKVCKYVDLPLQHISDRVLKAMHRGISRSET
                     IALVEKLRQNIPGVAIRTTFLVGHPGEDQEDFDELMSFVDEFRLERVGVFTYSEEEGT
                     YAQQYLADTIPPDEKERRASELMELQRNISFMINHSRVGSIIRVIVDSVEDEMIICRS
                     EFDSPEVDQNVNIHLHGYSQNLKPGDFVNVKIVDADDYDLIGELC"
     gene            complement(15779..16732)
                     /gene="ftsY"
                     /locus_tag="PK502_03575"
     CDS             complement(15779..16732)
                     /gene="ftsY"
                     /locus_tag="PK502_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPG99929.1"
                     /translation="MGIPNIFSTGKGNSRENLDKGLEKTRESLLKKVSRAVVGKSRID
                     DEVLDKLEEALISSDVGVETTLKIIQQIQQRAAKEKYLNANELNTILKTEIISLLADT
                     QVNSGAITELPSKPFVIMVVGVNGVGKTTTIGKLAYLYKLAGKKVLLGAADTFRAAAV
                     DQLTVWSERVDVPIVRQQMGADPSAVAYDTLNSAKANDSDVVIIDTAGRLHNKVNLMN
                     ELTKIRRVMQKVIPDAPHEVLLILDGSTGQNAFAQAREFIAATEVTGIVLTKLDGTAK
                     GGVVLGISDQFKIPVKYIGVGEKLADLQVFDKNAFVSSLFS"
     gene            complement(16845..16997)
                     /locus_tag="PK502_03580"
     CDS             complement(16845..16997)
                     /locus_tag="PK502_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HPG99930.1"
                     /translation="MAKKVVASMQQKGAKNFTRCIKMVKSPKTGAYTFKEEMVANTEI
                     DAYLKK"
     gene            complement(17005..17187)
                     /gene="rpmG"
                     /locus_tag="PK502_03585"
     CDS             complement(17005..17187)
                     /gene="rpmG"
                     /locus_tag="PK502_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPG99931.1"
                     /translation="MAKKGNRVQVILECTEHKESGMPGTSRYITTKNKKNTPDRLERR
                     KYNPILKRVTVHREIK"
     gene            complement(17200..17442)
                     /gene="rpmB"
                     /locus_tag="PK502_03590"
     CDS             complement(17200..17442)
                     /gene="rpmB"
                     /locus_tag="PK502_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPG99932.1"
                     /translation="MSRVCQITGKKKMVGNNVSHSKRRTKRRFYPNLKKKRFFLEEEN
                     RWVTLKVSAAAIRTINKNGLKATLNKAVEKGILKTY"
     gene            complement(17506..18780)
                     /locus_tag="PK502_03595"
     CDS             complement(17506..18780)
                     /locus_tag="PK502_03595"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HPG99933.1"
                     /translation="MNAGIITIGDELLIGQVVDTNSAWIATRLNSLGFAVERILSVSD
                     TEQAICESVNEAIDRYDLTIVTGGLGPTSDDVTKESLRRIFGGEMITDAQSLELIEIF
                     CSKRGIAVTETNRKQADVPSSCTVLPNYFGTAPGMLFKKGIHMLVSLPGVPFEMKALF
                     ENEVTPLLKQLPVEGVIIHKVIHTFGIAESNLSDVLNEFEKSISPSIKLAYLPDPSGI
                     RLRLSAYGNDRKLLSQQVDEAIEKLRATIPQYIFGYGETSLPEVVGKMLLSCNATVSV
                     AESCTGGKISHLIVEIPGASSYFNGGVVAYSNQVKENLLGVKHQTLIKYGAVSREAVE
                     EMAVGSLNLLSTDYSIAVSGIAGPDGGTPDKPAGLVWIAAASVNSLEVEKYIFSNDRS
                     INIQRASYSALNMLRKLLLKEHPELKNIFEKV"
     gene            complement(18840..18915)
                     /locus_tag="PK502_03600"
     tRNA            complement(18840..18915)
                     /locus_tag="PK502_03600"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18880..18882),aa:Thr,seq:cgt)
     gene            complement(19159..20145)
                     /locus_tag="PK502_03605"
     CDS             complement(19159..20145)
                     /locus_tag="PK502_03605"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HPG99934.1"
                     /translation="MGRIRFCGLFLLLFVSACISYRQIDIEYYDSSTKISIPTGNGKV
                     LILTNYQGKSHSSGGETLAWALDSVAATEATLSLVKVLNSSPAFSQTSFSHYAVYRKD
                     TSNMILPLPWTQLKNLSEINDSAGLILSLDYMNVKPTAGSYTLWQGSVNAYHGYIYVP
                     VYCYWRVYDITNQKITNSFLHRDTLVWEADDWREVKPGDQLPGYFAAVAYSGADAAEK
                     FAAQIAPFWENSTRILYNSGSKQMVEAEKLALKGDWRGAAEIWQQVIKNGGKALPSRA
                     AFNMALANEMLGNFNVSLEWLMQAKRLNPNLEGLNDYMEIINYRIKANSTKP"
     gene            complement(20259..21005)
                     /gene="fabG"
                     /locus_tag="PK502_03610"
     CDS             complement(20259..21005)
                     /gene="fabG"
                     /locus_tag="PK502_03610"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPG99935.1"
                     /translation="MKLLEGKTSLITGAARGIGRAIALAYAREGSNVAFTDMVEDDNM
                     KSLERELNALGVRAKGYVSDASNHENSQMVVDQIATEFGRIDILVNNAGITRDTLLMR
                     MSEEQWDLVIRVNLKSVFNLTKAVQKYMIKQKSGSIINMSSVVGVSGNAGQANYAASK
                     AGIIGFTKSVAKELGSRNIRSNAVAPGFIITDMTHQLSEEVRQDWIKKIPLQRGGTPE
                     DVANVCIFLGSDLSSYVSGQVIHVCGGMNT"
     gene            21450..>22124
                     /locus_tag="PK502_03615"
     CDS             21450..>22124
                     /locus_tag="PK502_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPG99936.1"
                     /translation="MKVRLKLRSKLLLSVLSTAIVVFLVSFGYLTNKLEDITLRSAFR
                     YVDATTREHSNAVKANFDEEMGMARAMAQGFQNYKDLNYEEINKNSLRVLQGIAVNNP
                     QFLSVWASWELNSIDRTWDKPYGRVRYTYFWENGALKYVEDRVNLTGDDPNSSYYHIK
                     STKREFIIEPYWFTYTGTNMKVLETSICVPLVDNDRFVALFGFDIELERYHEFIDSVE
                     IFPGSNS"
BASE COUNT         6410 a         4704 c         4524 g         6486 t
ORIGIN      
        1 tgttctattt aaataatgtt taaatagaac tgtaggagta ggtagcctaa agtgctataa
       61 aatatcccga ttcaagcagg ttaagcgtgg ctaaaataag cgtagcttca tttttaggca
      121 taaagaatag agtttacccc gctactatgg gaactaacta tttttaaaaa cttcagccct
      181 aaatttttat tggttacata tgtaatcgtt ttaaaatttt gatttgccgg ttataaaatt
      241 tacatttgta tgctcaacgt taaaatcaga tcgctaaatg gagatgaagg atagaatacg
      301 gttattgctg gatgcagaaa aaattaactc ggctaaattt gccgaagcaa tagaagtgca
      361 aagaagcgct gtttcacaca tactagcagg cagaaacaac cccagctttg atattatcca
      421 gcgaatcgcc caaaagttcc ccaaaattaa tcttgagtgg ttgattaccg gaaagggaag
      481 catgtacaaa atccccgttc aacgatcaat ttttgatacg gacgattcaa ttaatcaacc
      541 aggtaaaaca gcccctacgg caaatttgac agaaagtcga acgattacaa atgtaaacaa
      601 caggggtgag ggtggaaaaa ttttatccgg tcgatatgaa ggtgccgatt cggaggatac
      661 ctcaaaagaa cttacggtgc aaaaaattgt tatattttat tctgaccgca cgtttgtgga
      721 gtataggcca tcaatatcct gaattttctc tttcaatttt cttacttttg tagaaaattt
      781 ggtcatgtac aaatccattt ttaggaagtt gctatttatt tttccacccg aaaccgttca
      841 caagctggtt gtttccctca taaaattttt tttcaaaatt cctggagcaa gaaatgcttt
      901 taggagaatt ttttccaata gaaatgaggc atgtttagaa agagaagtaa taggcttaaa
      961 attttctaac ccggtaggac ttgctgctgg attcgataaa aatgccgaat tttttaacga
     1021 attttcttca tttggatttt cgtttatcga aatcggcacg gttacgccag aaccccaacc
     1081 cgggaacagc agacctcgcc tttttagact tttacccgat aatgcgttgg ttaaccgaat
     1141 ggggctaaac aataagggag ttaactatgt taagtcgaga ttgcagcaca aggaaaacca
     1201 aggtttaatt attggtggaa atattggaaa gaactcatct acgcctaata gtaatgccgt
     1261 ttccgactac attgcatgct ttaatgagct ttactcattt gttgactatg ttgccgttaa
     1321 ccttagctgc ccgaatgttc ataacctcag cgacctacaa aatcccgata gcgtgaagct
     1381 aattattgaa tcgctggtta atgaaagggc aaaatttcct tcgaaaaagc ctattctcct
     1441 caaggtctcg cccgacttga gccctgaaca gcttaaccaa acaattgatg cagctgaaga
     1501 ggctggtgtg gatggctata tcgccaccaa taccactact ggacgtaaca accttaaaac
     1561 taaaaaaagc gaactcgaga agattgggag aggaggactt agcggcaagc cgctccgcga
     1621 tgtttcaact gaggttatcc gcttcataaa ccaaaaactt gacgggaaaa agccgataat
     1681 tggcgttggg ggtattttca ccccaatgga tgcgttggaa aaacttgaag ctggagcatc
     1741 gctggttcaa gtatacacag gtttcatata tgagggtcca tttattgtta agagaataaa
     1801 taagctattg ataaataaaa tgttttacac atatatataa atagaaatgg ctaaacttgt
     1861 tgattgcagg ggaatggtct gcccccaacc cctggttgaa accaggcggg aaatcaagaa
     1921 atctaatcct ggtgaggcca taacggtggt ggtggataat agcacttcac ttaaaaatat
     1981 cctccgtttt ttagaggaca atggggttga atgcgttgtg aagcaagaag cagaaagcag
     2041 ctcaattacc tttaatgttc ctttacaaat gactcaaccg gataagagag cagaggaata
     2101 ctgcgatgtt tctcccaaac ctgatgtcaa ttccggtttt atcgtactgc tgaatagcgc
     2161 cacaatgggt aagggaaacg acgagttggg taaaattctt atgaaagggt ttctcagcac
     2221 tcttctggag cttaaagcta caccaaccga aataatctgt tataatagtg cggtaaccct
     2281 tgcccttaaa agttccgaca ctgctgcaac tcttcaacaa atttctgaaa gaggtataaa
     2341 aattactctt tgtggaacct gcgttgactt ctacaactta agggacgatt tggccgtggg
     2401 ccaaatttcc aatatgctct atattgccga aaggctgtca tcgggtgtga ggataattca
     2461 accctaatat tattcatatc atttttattt ctagttgatt accttattaa actgaaagta
     2521 gttaattagg tagattaagt aataaaatga acctgtactt cgataactca tcaacaagct
     2581 tccctaaacc cgaaggggtt agccaatcca tcaacgaata ctttagccat ggtggtacct
     2641 atggaagagc agcctacaat cgtgttatcg aggtttcccg taaggttgaa tctacccgta
     2701 gcctattggc caaccttatt ggaaccagcc ttgcccccaa tgtggttttt acttcaaatg
     2761 ccactcatgc tattaacttg tttatcagag gaatgcgata ccccaagagg agggtaatta
     2821 tttctccact tgagcataac gccgtttgca ggcctctaca atacttattc cagactgcag
     2881 gggtcggagt tgatattttc cctcatcatc ccgatgggag aattgatatt caaaaagccg
     2941 ctaaggttaa ctttgcaagc tacgatttgg gaattatcaa ccatgtaagc aacgtgaatg
     3001 gggtaattca gcccataaag gagataaaaa ggttaatggg taacacacca ctcctggttg
     3061 acgccagcca gtcggccggg agggtcgaga taagagctga cgaatggaag ctagatgctt
     3121 tagctcttac cggccataag ggtatcatgg ggccaactgg aaccggtgcc ctattcatcc
     3181 gcaatcccga catggccgat cctttaattt tcggtggaac aggatcaaat tccgagagct
     3241 acctgcaacc catcgaccta cccgataggt ttgaagccgg aacacctaat atcgtcggaa
     3301 tctacggttt atggggagct ttatcatcgg gtatgggcta ctcttttaaa cgcgagcact
     3361 tcgtagagct cctgcacgaa atttctacat tttcttcctt taaacttttg gcggctgacg
     3421 acacggactt ccaatcggac gtcttctcca ttaaacccct tggaatggag gtgtcagaac
     3481 ttgctcgcat tctctttgaa aaattctcgg ttgaaacccg aagtgggcta cactgtgcac
     3541 ctcttgccca tcgcacgttg ggaacttttc ctaccggagc cgtacggttt tccctgtcaa
     3601 agtttcatac tgataacgac ctcgactact tgcatagctg cttaaaaaaa attgatgagg
     3661 gaagaagcta acacaggatt tacaattctc atattcagca acatccacca ggtaatgcat
     3721 gccgaaaaga ttattgctga caagggttat ccgtacagaa tagtccccgt tccatcaaac
     3781 ctcagctcgg aatgtggttt atgtataaga attgaaagtt catatttgga tagcattcta
     3841 tcagatttac agaatattaa ggtcgagatt cataccctaa ataatgaacc ttaaccacct
     3901 ttaaaaagaa tataaattac aagatgatgg aagaatttga cttactatca accgtggagc
     3961 aggggggatg ttcctccaag attcctgctg cattgctcga atcccttctt cccgcattgc
     4021 caaagcttgt tgatgaaaac attctggttg attttagctc ccatgatgat gccggggttt
     4081 accggatttc agacgatttt gcgctgattc taactaccga ttttttccct ccggtttgtt
     4141 ccgatccttt tgagtttggc caaatatccg cagcaaattc tattagcgat gtatacgcga
     4201 tggggggaga gccattcctc gcccttaaca tttctatgtt cccctcttcc aggataccca
     4261 ttgatgttta caagtctata cttgaagggg ccgcttcaaa agcgctggag gcaaatgttg
     4321 caatcatcgg aggtcatacc attgatgatt tccccccaaa atttggcttg gctgttgttg
     4381 gccgtgttga tcctaagcgg ttggttacca actccgggct tcgccctggt gataagctga
     4441 ttctcaccaa gcccctggga accggcatta ccgtagcggc aaaacggctt aaaatggtta
     4501 cagacgaatc gtaccgaatt gccattcaat ggatgaaaac attaaacgac agagcctgca
     4561 gcgttatgaa aaaatatcat gttaccgggg caaccgatat taccggcttt gggcttttag
     4621 gtcatttgct tcgaatggcc aggtccagca gagtaaacat ttcaatccaa gccggcacgc
     4681 ttccggttct gcccggagtt accagcctgg tgaacgatgg ctgcattccc ggtgcagcct
     4741 tccgcaacct ggagtatgta gaaaacgatg taaccttcac ccgcgttgac tacgacttaa
     4801 aaatgataat ggttgatgct caaacatcag gaggcctgct gatgggagtt caggacgaat
     4861 ttgccaatca aacccttcag gagctgactg atgctggact ttgctactcc agaattattg
     4921 gcgaggtggg agaggggaat ggtaaaattt tcgtttcttg atggttattg gtaatcataa
     4981 caaacataca ttttataata aatatttgaa tatcaatata atatgaattt ttttcgaagt
     5041 agaaatggat attgtagcag caggtagtaa aatttgaatt tacagtgcaa tgttgtcatg
     5101 caataatgct ggaataagag gtcattaggt cgtttactaa atcaaatttt atgaagctta
     5161 caaaggattc ctggattacg tttacaatcg aacgactcgt tgaaattcct caggcaggat
     5221 attactaccg tttgcttcat gaatcgggac aaaaatttct tctgcctgcc cagtactaca
     5281 ctcattataa ccttgtagtt ggaagcacag tcagctgccg ggttgataag attaactgca
     5341 tggggaaagt attccttgaa ccaaaacatc cttactatga agttaacatg gtttacaact
     5401 tcgattttgt agccatgaat aattcttttg ttaagggtgt tgccgagctg gttgtaaggg
     5461 acatttttaa cgagacctac acgttattcc ttcctgctga ggtctgctca aaaatatcaa
     5521 acatggttcc tcttagggta ctcaacataa ggaaggggag gttacagctg cagcttgcag
     5581 gagcaacagg tagctgcaag gaatttgagg aaaacgaaat cattgacctt actattgaga
     5641 cggttgttga aattaatgga gaaaaattct tttttctaag ccatagcagc gggtgttttg
     5701 ctttattaaa agtaaagtac taccttcatt acgattttga agttggaaag caagttagct
     5761 gccagtatat tgggataaaa ccagacggaa ccttaagagt tgagcctcca aaccctttct
     5821 acagggtagg tgatacctat aactttacgg ttagcagtgt aggtacagaa agcaacgctg
     5881 atggtgaaac agctagctat gcaacagttt tcgatatata tgggatgaag tgtggttttg
     5941 ttgttcctgc aggcagtaag gtgcaacccg atcaagtcat actatgtaaa gttgtagggt
     6001 acaaaaaagg acgacccatg cttatgatcg tccaaaaatg agagcatata ccagttaagt
     6061 caacctataa atcatcgctc atttcctcgg atggctcatc ctcttcattc atatcgtccg
     6121 gaaaggcatc gttttcgcta ccaatgtcat cgcttaagcc tccaaaaaat tccttttcgt
     6181 cttcctcatc ctttatttta gagtcgattt taacatttac ttttaccagg tagctagcat
     6241 catcggaatc gagggtaaca gcaaaaaaat aatcacctcc cggcttttcc accttaatta
     6301 catgattctg atatccatgt ggatattttt gcttaaaaag ttccaatagc tccggtgaaa
     6361 gatcaccaaa tttgataaca actcttttct tagccatacc ctgaaaaaaa tttttgcaat
     6421 tatacaccaa aattaaataa tgcaaacgag ccctgtaaaa ataatttcat tttttaatag
     6481 aagcacaatc agtaaacgat tactaccttt gggtagaatt ttatagcaat gctttaatgc
     6541 attttttctc aacattaact atttaacata tcgtgatgat aaacaaatta ttgctagttg
     6601 tgacttgcgt tgttcctata ctgtttgggt acaacgctat ggctcaggtt caaatttaca
     6661 atcccaatgc tgatgccatc aaggatttaa atcaggctat tgaaagggct ggcgttcagg
     6721 gtaagcatgt tctggtgcag gtaggtggaa attggtgccc ttggtgtgtg aagctgaata
     6781 agattttcac gacagattca accattatga aggtgttgaa cgcaaattac gtgctggtta
     6841 aggttaacta cagcaaggaa aataagaacg aagcggttct tgaaaagctg gaatttcctc
     6901 agcgttttgg atttcccgtg ctggtaatac ttgacgatca tggcaagaga attcacactc
     6961 aagattccgg attgctggaa tccggagatg gttacgatac aaaaaagata gtaagctttc
     7021 ttaaaaactg gacaaaagcg gccatcggaa aaccggcaaa ttagtcctat aattaatatt
     7081 atgttaaata taggtattta aaaaagaccc cgactttatc ggggtctttt atactacata
     7141 ttgtttagct tgtcgataat ctccttatac tttgcactca tttccggact ttcatttcta
     7201 aagcggaagt aaatgttctt tagtgcatcc ataacctcct tgctggcggg gttgattttc
     7261 agagcgtttt ccatataagg aagagatttt ttgaattcaa cattagcctt tgacataagt
     7321 tcatcatact tttcaatctc cttagctgga atcttattgg cttcttctac atactttaca
     7381 cctacattga agaaaagcgc tccaatgttg tagtaggcat caaagtagtt ggggtcaatt
     7441 tctattgcct tttggtatga agttaaggct tcgtcagtat cgccaagcct atcatgcagt
     7501 gtccctttgg caaagaaaag tgaagggtta ttaggttcgt ttgcaatagc ttggtctatg
     7561 taaaccaaaa ttttcgaagg gtcttccccc ttatcaatgt agtagctaat aagtgaaaaa
     7621 agaacattgg tgtttttagg atatttaata aatccatctt ccaggtattt agcgccttcg
     7681 tcagccttac ctagtgattt gtatgcgtca aaaatgttga agtaaacgct accctccgag
     7741 gtgaaaccca gcttaataga ctcttcatag tagggaatgc attcttcaaa tttcccatcg
     7801 agctgtgcag ataacccagc atagtagtat attgcggtgt ctttctgatt aactaagggg
     7861 tttttgccaa tttcaacaac cttactaaag catttatatg agcagccata atcttttttg
     7921 ctgtagcagc ttgacccttc gttgattaag tagttcttca attttagcaa gtcttccatt
     7981 attggcttgg cctttttctt gcttgcatca agctcaatgg ctttgatcaa gctttcgtat
     8041 gctacctcca aaggattctc aacaacaggt ttagttacct gccagtgatc taatgagccg
     8101 ttagcaaagt aaaattcagc tctatccatc acgaacttat ccacggtaat gccttcaaca
     8161 acttcctgag tttgctcagt tggtttacca attagtaatg aaaatgtttt catgtccatt
     8221 ccagggtatg cccggtaaat ctgagcatca aatacatcca taaacaactc tgccctagca
     8281 atccaagcat cgggtttcgc attttttttg ggatcctgaa tggtggcgtt acttttctca
     8341 attttttttc caaactgatc aaagttaatt tgtacgccag tttgtgcgtt gagcattccc
     8401 atacccagca cagaaaatag tagtacagtt gttaatcgtt tcatttcatg taaattagtt
     8461 aaagttaaaa attaaattca aaaataaatt atgtgtttgg gtttttcatt gttcgctttc
     8521 tccttcttga actaaatttt gctcatctgg ttcattttca gatgattcaa cattggctat
     8581 tgcagcgata ctatcattct tttttaggtt tatcagccta actccctggg catttcgacc
     8641 cataacccta agttcgctga ttttaagcct tatggtaagc ccgctgcgat taataatcat
     8701 taagtcgtta ttttcatcaa ccgctttaat gctaataagc ttccctgttt taggggtgat
     8761 gttaatggtt ttcacccctt tcccacccct gttggttatt ctatagtcct ccaacctgga
     8821 tcgcttgccg taaccatttt cagaaacaac aaggatatct tttgatgcat tttcctcaac
     8881 acaaaccatg ccaaccactt cgtcgtcagg ggaaatagaa attgccctaa ccccactacc
     8941 agttctacct atcgggcgga caagatgttc ggggaatcgt atggcctttc catttcttgc
     9001 agcaatcagt atgtggcttt tgccatctgt gaggcaagcc tcaataagct ggtcgccctc
     9061 cttaacggtt accgcaataa caccgttttg acgtggccgg ctgtaagcct caagaggcgt
     9121 ttttttaata atgcctcttt tggtacacag cacgatgtag ttgttcttga gataatctgt
     9181 gtcatcaagc cctttgacat tgatgtaggc tctaactaca tcgtcatttt caatgtttat
     9241 gagattctga atagctcttc cttttgatga tttagtaccc tcaggaattt cataaacttt
     9301 tagccagaaa catttgccgt tttcggtaaa aaacagcatg gtattgtgca tagtagcaac
     9361 atacaggtgc tcaataaaat cctcatcacg tgtatcgcta cccttagccc ctacaccacc
     9421 cctcgattgg agcttatact cataaagcgg ggttcgttta atgtagccca tgtgcgagat
     9481 ggttatgaca acttcctcat cggcataaaa atcttcaggg ttaaactcct cggcgtctgg
     9541 aattatctct gtttttcggg catctccata cttttgcttt acctcaacca gctcatcctt
     9601 aataatcttc atttgcaggg ctttatcctc aagcacctgt ttaagatagg ctatcagctt
     9661 ttggagctca ttgtactcct cctttacttt ctcacgttca agtcctgtaa ggctgcggag
     9721 ccgcatatca ataatagcct gggactgaat atctgaaagg ccaaatcgtt caactagttt
     9781 ttccttcgct tcggccggtg attgggctga acggataagg ttaataacct cgtcaatatg
     9841 atcaatggct ataagcaggc cttcaagtat gtgggctctt ttttcggcct cctccagttc
     9901 gaaccttgac ctgcgaataa ttacgtcatg tcggtgcttg ataaagtgaa caattaggtc
     9961 cttaaggttc aacagcttcg gccttccatc aaccagggca atgttgttga tggcaaatga
    10021 tgactgcagc tgtgaatgct taaaaaggtt gtttactacg acattagcaa ccgcttcctt
    10081 tttaagaatt attacaaccc tcatcccatc cctatccgac tcatcgttga tgtagctaat
    10141 accatcaatt tttttctcgt ttataaggtc ggcaatccga cggatcatct cggccttatt
    10201 taccatgtag ggtatttcgg ttacaattat tttggacctt cccgactcgg tttgctcaat
    10261 ttctattttt gaacgaacca caattttacc cttgccggtc cggtaggctt ccttaacgcc
    10321 ctgcgtgcca taaataaccc ctccggttgg gaaatcagga cctttaacat actttaacag
    10381 ctcatcagta gttatatcag gactatctat tgtagcacaa atagcgtcaa caacttcgcc
    10441 caggttatga ggaggcatgt tagtagccat tccaacggca atacccgatg tgccgttaac
    10501 cagcaacaag ggaattttag ttggtaaaac agatggctcc tcaagggtat cgtcaaagtt
    10561 aaggttgaaa tctacagttt gtttatccag gtcagctaga acatcctctg caattttagc
    10621 caggcgggct tcagtgtagc gcattgccgc tgggctatcc ccgtcaatag acccgaaatt
    10681 accctgccca tcaactagcg ggtaccgcat ggaccaatcc tgggccatcc tcaccatagc
    10741 atcgtaaacc gaagaatcgc catggggatg atactttcct aaaacctccc ctactatacg
    10801 tgctgatttc ttgtagggat ttcctgcatt caaacctaaa tcggacattc cgaacaatac
    10861 ccttcgatga accggcttaa gtccatcccg tacgtcgggc agtgctcttg atacgatcac
    10921 cgacattgag tagtcaatgt acgatgactt catctcctcc tcaatgttaa ttctaataat
    10981 cctctctgtt tctgacatat atacttttta taaaaagtaa attctgaata tcgaaccaaa
    11041 aaaatttgaa agtgctaagg tagtaattta atccattgta tagccctgaa accctttttc
    11101 gtttttgaga aaactcacga taatttaata atttttatca acaaatcgct tgcttttccg
    11161 tttatcacta ttattttagt accaaattta acttaagcaa gtttttatga accaaaagtt
    11221 ttcacaaaaa atcagagatg tactttcata tagccgagaa gaggctatac gacttggcag
    11281 ttccgcaatt agctcagatc accttttctt gggtattctg cgcgatggag aaggcgaagc
    11341 aataaatatt ttgctggccg aaggtgttaa tttaagcgag ctgaaaaagg aactggatgt
    11401 ccatttgctg caaatcccaa gtaaggcaga ggaaataaac gactcatcgg aaattgcaat
    11461 ttataagtcg gccgaaaggg cgttgaaaat agttattctt gaggccaggg cattaaaaag
    11521 tgagtcaatc aatacagccc atctcctgct tgccatactt aaggatgata ccaactatgt
    11581 aacccagctt ttaaaggaaa aggatattga ttacgaaagg gttaggaaaa atctgacaat
    11641 attgaactcg gacgataaag gtgattttgg ttctggagat gaagatgagg gggatagtgg
    11701 ttttttcggt aacatgggca aatccaacca gccctcaaca ggaaaatcag cctccgacac
    11761 ccccgttctt gacaattttg gaattgactt aactaaagct gccgaggatg gacgtcttga
    11821 tcctatagtt ggccgtgaaa gggaaattga aagaattgcc caaatcctaa gccgtcgaaa
    11881 aaagaataac ccaattctta ttggagagcc cggagttggc aaatcagcca tagccgaagg
    11941 gctggctctt cgaattgtaa agaaaaaagt atccagggtt cttttcaaca aacgggtaat
    12001 cacacttgat ctggcttcta ttgttgccgg gaccaagtac cgtggccagt tcgaagaacg
    12061 gatgaaggcg atactcaacg agctatcgag gactcccaat atcatcctgt ttattgatga
    12121 aattcatacc attgttggtg caggtggagc tacgggctct ctcgatgctg ccaatatgct
    12181 taaaccggca cttgcaaggg gtgagcttca gtgcataggt gcaactacac tagacgagta
    12241 tagggagcat attgaaaaag acggggcttt agagcgtcgg ttccagaaag taatgattga
    12301 acctacctct cctgaggaaa caatcgaaat tcttaacaac atcaaagatc ggtacgagga
    12361 gcaccataat gttacctaca cacctcaggc aattgaggct tgtgtaaagc ttaccatgcg
    12421 ctacatttcc gacaggaatc taccggataa ggctatagat gccatggacg aagctggctc
    12481 acgggttcat atctcgaata ttcgtgtgcc cgatactatt gttaacctag agaagctgat
    12541 tgaggaaacc aaaaaggaga aaataaaagc cgttaagaat caacaatttg agttggctgc
    12601 taatcttagg gataaggaga aaaagctgat tgagcagctt gaggaggagc agtcgagatg
    12661 ggaaaaggag ctggctaagc atcgtgaaat tgttgacgag gaaaaggtag ccgaagtggt
    12721 tgccatgatg acaggagtcc ctgtgcaacg aatagcacaa gctgagggag ccagactgct
    12781 caaaatggca gatgagctaa ggaattcggt aattgggcag gatgaagcca ttcagaaggt
    12841 agttaaggct atccaacgaa accgtgctgg acttaaagat cccaacaaac ccatcgggac
    12901 atttatcttt ttggggccaa caggcgttgg caaaacccag ctagctaaag tgctcgccaa
    12961 gtatcttttt gatactaccg ataaccttat taggattgac atgagcgagt acatggaaaa
    13021 attttccgtg tcacgtttgg ttggtgcacc tccaggttac ataggatatg aggagggtgg
    13081 gcagctgacc gaaaaggtta gaagaaagcc ctactcggtt gtgctgctag atgaggttga
    13141 aaaagctcat cccgacgttt ttcatatcct gctccaggtt ctggatgaag gccagcttac
    13201 agatagcctg ggcagacggg ttgattttaa gaataccatc atcatcatga cttctaacat
    13261 tggctcccgg gagcttaagg attttggtca aggcgtgggt tttaccacca aagccaaaga
    13321 acaaagcagc agcagcgatt attcaagaag tataattgaa aaagcgctta aaaaggcctt
    13381 tgcaccggag tttctgaata gaattgatga tattgtaatg ttcaactcgc taagtcgtgc
    13441 tgatattcat aaaattattg atattgagct tagcggcctt tatgagcgga ttcgtaacct
    13501 gggattcaca attagaattt ctccggcagc caaggatttt attgctgaga agggatatga
    13561 tgttcagtat ggtgctcgcc ctcttaaacg agccatccaa aaataccttg aggatgccct
    13621 ggccgaagtg ctcattaaaa gtgataccaa gcccggatgc acaatctccg ttggatataa
    13681 caaaaagaag gatgagattt ccatcaaagt tcttaagggg gaagaggagg taaccagcgc
    13741 tagtgaagag gagtagaacc tgaaggctaa tgaacaaaaa agtttcggct gtgttactac
    13801 caaaattaca tcactaaaca ccataaaaac catgaacgtt acttttggag gaaaaacagt
    13861 tacccttgaa ggtgaacaag ttaaagtggg ctctactgct cctgatttta cagcactcgg
    13921 caccgacctt aaacctgtca agctctctga ttttggcaaa aaagttaagc taatttccgt
    13981 gttcccttct atagatacgg agatttgctc aattcaaaat catcgcttca ataacgaagc
    14041 atcttcattt ggcgataagg ttgcttttat tgccatttcg aatgacttac cctttgcctt
    14101 gaagcgattc tgcggagccg aaaaaattac caaccttacc accctatccg atcacaagga
    14161 tgttgatttt ggtaagaaat ttggtttttt gataaaggag ctacgcttgc tgactagagg
    14221 ggtgattatt ctggacaagg ataacaaagt tcaatatgtg gagatagtcc ccgaaatagc
    14281 ctccgagccc aactatactg aagcaatagc tcgcctaaag cagctagttt aaaaaaagct
    14341 cgacttggag gtgcatttcc aataaaagga aatttccaca agtaaaagaa aagccttaga
    14401 aaggtaatca ttctaaggct tattttttat ccacaccaac ctcacaagag ctcagcacaa
    14461 ctcccctatc aagtcataat cgtctgcatc aacaatttta acattcacaa agtcgcctgg
    14521 tttgagattt tgtgaatagc catgaagatg gatatttaca ttttggtcaa cttcaggaga
    14581 gtcaaactcc gatcggcaaa taatcatttc atcttctacc gaatcaacta taacacgtat
    14641 aatgcttcca actctactat gattaatcat gaacgaaatg ttgcgttgaa gctccataag
    14701 ctcggaggca cgccgttcct tttcgtccgg gggtatggta tcggctaaat actgctgcgc
    14761 ataggtcccc tcctcctctg agtaggtgaa tacccctacc ctctctagcc taaactcatc
    14821 cacaaagctc atcaactcgt caaaatcctc ttggtcctca cctgggtggc caacaagaaa
    14881 cgttgttcta attgccacac ctggaatatt ttgtcgcaac ttttcgacaa gtgcaatggt
    14941 ttccgatcgg gagattcccc ggtgcatggc tttcagcacc ctgtcactaa tatgttgtag
    15001 gggtaaatca acatatttgc aaacctttgg gttgtttgca atagtggcta taagatcgtc
    15061 tggaaaatga gcgggatagg catagtgaag cctaatccac tcaattcctt tgatttggct
    15121 caaccgatcg attagctgag caattctccg ttccccatag atgtccatac catagtaagt
    15181 tgaatcctgg gcgataagta tgagttcttt cacccctttt tctgatagga tggtagcttc
    15241 ttcaacaagg gattcgacgg ttcttgaaat atgcttacct ctaattaacg gaatggcaca
    15301 gtatgagcag ccccagctac atccctcgga aattttcagg taagcataat ggccgggtgt
    15361 tgtgagtaac ctggtggtaa gacacccgct aaaaacgggg gtgttgagag catggagaac
    15421 ggcggggagc tcggaaactc caaaaaatcc atccacctcc ggaacttcat cctgcagctc
    15481 atttctgtat ctttgagcaa gacaacccat aacgaaaacc ttgtcaatga tgcccttatt
    15541 tttcgcatct acaaagccca tgatggtatc aacggactct tccttggcat cggcaataaa
    15601 cccgcaagta tttattatca ccaccttagc ctcagtatcg ttcgaatcgt gaacgacctc
    15661 ccatcccccc attttaagct gattcatgag tacttcggaa tcaaccaaat ttttagagca
    15721 tcctagggta accacattaa tttttccctt tcccggttgt tgcatactgc taatttttct
    15781 agctgaaaag ggatgaaaca aatgcgttct tgtcaaaaac ctgcaggtca gccagctttt
    15841 ctccaacgcc aatgtactta actggtattt taaactggtc ggaaatgccc agcacaacac
    15901 ctcccttggc tgttccatcg agcttggtta ggacgatacc ggtaacttca gtagcagcaa
    15961 taaattccct ggcttgagcg aatgcgtttt gtccggtaga gccatcaaga attaacagca
    16021 cttcgtgtgg tgcatcggga ataaccttct gcataactcg cctgatttta gtgagctcat
    16081 tcatcaggtt aaccttgttg tgcaacctac ctgctgtgtc aataataact acgtcagaat
    16141 catttgcctt tgcagaattc aacgtgtcat aggctaccgc cgatggatcg gcgcccattt
    16201 gttgcctcac aatgggaaca tctaccctct ccgaccatac tgtaagctgg tcaacggctg
    16261 cagccctaaa cgtatctgct gcacccagta acaccttttt gccagctaat ttgtaaagat
    16321 acgccagctt gcctatggtg gtagttttac ccactccatt gacaccaacc accataatga
    16381 caaatggttt tgaaggcagc tccgtaattg cacctgaatt cacctgagta tcagctagca
    16441 aagaaataat ttcggtttta agtattgtat ttagctcatt ggcattcagg tatttttcct
    16501 tagccgctcg ttgctggatc tgctgaataa ttttcaaggt ggtctcaact ccaacatccg
    16561 aggatataag ggcttcctcc agcttatcga gcacttcgtc gtcaattcta gattttccaa
    16621 caacagccct tgaaactttc ttcaacaagc tttctcgggt tttttccagc cccttatcga
    16681 gattttcccg gctgttgccc ttacccgttg aaaaaatatt tggtattccc atttcaaacc
    16741 ttgttaagtg ctatgaatat ggtattggtt ataaaagata aaaaaagctt tccttttgca
    16801 agaaaagcct aaatacggtg atacatttgg agaagcacac aggcttattt cttcaaatat
    16861 gcatcgattt cggtattggc aaccatttct tccttaaagg tgtaagcacc tgttttgggt
    16921 gattttacca ttttaatgca acgggtaaaa tttttagcac ccttttgctg catagaagca
    16981 actacttttt ttgccatggc tctactattt tatttcacga tgaacagtta ccctcttaag
    17041 aataggattg tacttgcgac gttcaagcct atcaggggtg tttttcttat tctttgtagt
    17101 tatatacctg gaagttcccg gcattccgct ttccttgtgc tcagtacatt caagtatgac
    17161 ctgaaccctg ttacccttct tagccatttg ttaacagatt tagtaggttt taagaattcc
    17221 tttttctaca gctttattaa gcgtagcttt cagtccattc ttattgattg ttcttattgc
    17281 agcagcagaa accttgaggg taacccaacg attttcctcc tcaaggaaaa accttttttt
    17341 cttgagatta gggtaaaatc tgcgcttagt cctcctttta gaatgggata cattgtttcc
    17401 caccattttc ttctttccgg ttatttgaca aactctcgac atcgtaatat tgttctaagt
    17461 aagttttcta aacaggtcgc aaatttcggc aaaattatga atttatcaaa ctttttcaaa
    17521 aatatttttc aactccgggt gttcttttag cagtaacttt cgaagcatgt tcagcgctga
    17581 gtatgatgcc ctttggatat ttatggatcg gtcgttgctg aaaatatatt tttcaacttc
    17641 cagggagttt accgaggcag ctgcaatcca tacaagccca gctggtttat ccggtgttcc
    17701 tccatcagga cctgctattc ccgaaacggc tatggagtaa tctgtggaaa gtagatttaa
    17761 agagccaacc gccatttctt ctactgcctc acgactaaca gcaccatact taattagtgt
    17821 ttgatgtttc actccaagga gattttcctt tacctggttg gagtaggcga caacaccgcc
    17881 attaaaatag ctagaagcac ccggaatttc tacgataagg tgtgaaattt ttcctcctgt
    17941 gcagctctcc gctacagaaa ccgttgcatt gcagcttaat agcattttcc caaccacttc
    18001 aggaagtgaa gtttcaccat acccaaagat atactgagga attgttgccc ggagtttttc
    18061 tatagcttca tcaacctgtt gtgataagag cttcctatcg tttccataag ccgaaaggcg
    18121 aagtcgaatg cccgaagggt caggcagata ggcaagctta atggaaggtg aaatgctctt
    18181 ttcgaactca ttaagcacat ccgaaaggtt tgactcggca atgccaaagg tatgtataac
    18241 cttatgaata ataacacctt caactgggag ttgctttagc aagggagtaa cctcattttc
    18301 aaaaagtgcc ttcatttcga agggtacacc tggaagggaa accagcatat ggattccctt
    18361 tttaaacaac atgccaggag ctgttccaaa atagttaggt agtacagtgc aggaagatgg
    18421 aacatccgcc tgcttacggt ttgtttcggt taccgctata ccacgtttag agcagaaaat
    18481 ttcaataagt tctaacgatt gggcgtcggt tatcatctcg ccaccaaata ttcttctcaa
    18541 actttccttg gttacatcat cgctggttgg ccctagccct ccggtaacta tggttaaatc
    18601 gtaccggtca atagcttcat ttaccgattc gcatatagcc tgctcggtgt ccgacacgga
    18661 tagtatcctt tcaacagcaa atcccaggct attgagcctt gtggcaatcc acgcagagtt
    18721 tgtatcaaca acctgcccta ttagtagctc gtcgcctatg gtaattatcc cggcattcat
    18781 aaggccaatg aaatttattc aggtagaaat aaaaaccgga caacggtttg ctgtccgact
    18841 ggagcctctc acgggacttg aacccgcgac ctgcggttta cgaaaccgct gctctaccag
    18901 ctgagctaaa gaggcatttg ctgcaaaaat acagcttttt tagagacttc caacttgaag
    18961 cgaccttcca tcttttaaaa atgaaaccct gccaaaacag ctaaaatgga gaaagaagta
    19021 ggggttagtt tttccatttc gaaaacacta ttaatcatat aacacttact atcaactgaa
    19081 tataccgatt tatacaaact aatagttaaa aaatttaatg ggaagtgcag aacctgtggt
    19141 agcttaccaa agctaaaatc aaggttttgt cgaattggct ttaattctat agttaattat
    19201 ttccatgtaa tcatttaagc cttcgagatt gggatttaga cgcttagcct gcatcagcca
    19261 ttctaaggaa acgttaaaat ttccgagcat ttcatttgca agagccatgt tgaatgcagc
    19321 cctggatgga agtgctttgc ccccattttt aattacttgc tgccatattt cagctgctcc
    19381 acgccaatca cccttaaggg caagtttttc tgcctccacc atttgctttg aaccgctgtt
    19441 gtaaaggatt cgggtactat tttcccagaa aggggcgatt tgggcagcaa acttttctgc
    19501 agcatcagcg ccactatagg ccacggcggc aaagtatccc ggcagctgat ctccgggttt
    19561 aacctcacgc caatcgtcag cttcccatac cagcgtgtcc cgatgcagga aggaattagt
    19621 aattttttgg tttgtaatat cgtaaaccct ccagtagcag taaacgggaa catagatata
    19681 accatgatag gcattcacgc taccctgcca taacgtatag ctgcctgctg taggcttaac
    19741 attcatgtaa tcaagtgaaa ggataagccc tgctgaatcg tttatttcag ataagttttt
    19801 cagctgtgtc caaggcaggg gtaaaatcat attggaagta tccttacggt aaaccgcata
    19861 gtggctgaag cttgtctggc tgaatgctgg cgaagagttc aataccttaa ccagcgataa
    19921 ggttgcctca gtagcggcta cactatctaa tgcccatgca agtgtttctc ccccactact
    19981 atggctttta ccttgatagt ttgtaaggat aaggactttg ccattaccgg ttggtatcga
    20041 tattttggta gacgagtcgt agtactcgat atcaatctgc ctgtaggaaa tacatgccga
    20101 aacgaaaagt agtagaaaaa gtccgcaaaa cctaattctt cccattggta agaatattac
    20161 taaaaatagc tgaattgatg ctatttagcg gagatgtgac ttccaaacaa aaaataaaca
    20221 gcatcaattc aagcagaatt cgatattaac aagtggtact atgtattcat tccaccacag
    20281 acatgaataa cctgtccgct aacataggac gaaagatctg aaccaaggaa aatgcaaaca
    20341 ttggccacat cctcaggagt tccaccccgc tgaagtggaa ttttcttgat ccaatcctga
    20401 cgaacctcct cggaaagctg gtgggtcata tcggtgatga tgaacccggg agcaacggca
    20461 tttgagcgga tatttcttga gccaagctcc tttgcaaccg acttggtaaa tccgattata
    20521 ccagcttttg aagcggcata gtttgcctga cccgcgttac ccgaaacgcc cactaccgag
    20581 ctcatattaa taattgaacc gcttttttgc ttaatcatgt acttctgaac agccttggtc
    20641 aggttaaata ctgacttcag gttgacccta atcactaaat cccactgttc ttcagacatt
    20701 ctcataagta gggtatcacg ggtaatacca gcattgttaa ccagaatgtc aattctgcca
    20761 aactcggttg caatctgatc aacaaccatt tgggaatttt catggttact tgcatctgaa
    20821 acgtaaccct tagccctaac accaagagca tttagttcgc gttcaaggct cttcatgttg
    20881 tcatcttcca ccatatcggt aaaggctaca tttgacccct cacgtgcata agcaagtgca
    20941 attgccctgc caattcccct ggcagcacct gtaattaggc tggtttttcc ttcaagtaat
    21001 ttcatggtta aatgaatttt tgttgtcccg acaaagttaa aaaaatcccc tattatggaa
    21061 gagctgtagc aagcaacatg ccataacagt atttgaaaat tttgttacag caatgacgga
    21121 atttacaata tttatgagta tgtgctttag tttcaggctt caactattga gttataatta
    21181 ttttaccaca tgagatgaat gggaaactgc atatggatag gttcgaaaaa taagcacata
    21241 gcaaataagc ttattttacc cagcttgcga gctcaatatt tttttttgaa atgtaaatat
    21301 cctatattca cgctttaaac gttttgaaaa acattggtta tagactattg ataatggcat
    21361 acatacctgc catttttaag gtaatccttc aatggatctg tagcggtatt ttgatacgaa
    21421 caagtagtaa tgcataaaga gtataaaaaa tgaaagttag gttgaagcta aggagcaaac
    21481 tgcttctttc agtgttatcg actgccatag tggtatttct agtttcattt ggatacctga
    21541 ctaataaact tgaagacatc acccttcgta gtgctttccg ctatgtggat gcaacaacaa
    21601 gagagcactc caatgccgtt aaggcgaact ttgatgagga gatgggcatg gcaagagcaa
    21661 tggctcaagg gtttcagaat tacaaggatt taaactatga ggagataaat aaaaattcgc
    21721 taagagtact acaggggata gcagttaata atccccaatt tttatcggtt tgggccagct
    21781 gggagttaaa ctctatagat aggacatggg ataagccata cggaagagta cgatacacct
    21841 atttctggga aaatggggcg cttaagtatg tagaagatag ggtaaatttg acaggggacg
    21901 atcctaacag ctcatactac cacattaagt caaccaaaag agaatttata attgaaccgt
    21961 actggtttac ctatactggg actaatatga aagtgctcga aaccagcatt tgtgtaccgc
    22021 tggttgacaa tgaccggttc gttgctctat ttggatttga tattgagctt gaacgttacc
    22081 atgaatttat tgacagcgtt gaaatattcc ctggtagcaa ttcg
//
LOCUS       DAOPHX010000029        22082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_2482_length_22082_cov_6.126754, whole genome shotgun sequence.
ACCESSION   DAOPHX010000029 DAOPHX010000000
VERSION     DAOPHX010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 22082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22082
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2482_length_22082_cov_6.126754"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PK502_03620"
     CDS             complement(<1..255)
                     /locus_tag="PK502_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99937.1"
                     /translation="MIRHISRFFLVLMASGTVTQLFSQTIEDGIRFSQTLNGGTARSI
                     SLGGAIGALGGDFTSLSINPAGLGVYKSGELTVTPSFKNQK"
     gene            complement(366..1739)
                     /locus_tag="PK502_03625"
     CDS             complement(366..1739)
                     /locus_tag="PK502_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99938.1"
                     /translation="MKTKSYIPMLALLGFTTLSCSSSLQVSNTSSWEDEIYRANTKTS
                     KTELAQDSKDSTPVQTNLNDYAQLDKKYADELAVNQETTQNDTISSDKENVNPYESVL
                     SDSYQESYERRLKGMEDPMYGLNDWSVYYSDSYRYAQAYDPNYYRLVVMGSQVWVEPW
                     YIYNSFSWPRTSFYFGFGYGWGYNPWSWYYDPFFYSPFNYYNPYYYSAWNFPGNYWIN
                     HGIEYSYLNSGNFYYGRRPGESSFNTSSRRTASGVTPTSSQAVTRQRGDVQSSSINPI
                     QSTKNTQTRVTPTYENNRRSAEGTQRSGDQITTPRRETGTTLSNPSSTRGSAVSSGTR
                     SERPTRSIGISEPTGRAYNPGYDKPRTSSSGTQSIQRPSTGRGTTTSPSGRSSSSPTY
                     NSPGRVSAPSNGSATRSGNSVQSKPEGSRSSGNSSGTYTPRTESSSSGSRGNSNSGSS
                     GAKTRGR"
     gene            1838..3313
                     /gene="proS"
                     /locus_tag="PK502_03630"
     CDS             1838..3313
                     /gene="proS"
                     /locus_tag="PK502_03630"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPG99939.1"
                     /translation="MAKEITSRNENYSQWYNDIVIKAGLADYSAVRGCMIIKPYGYAV
                     WEKIQAQLDRMFKETGHQNAYFPLFIPKSFFSREASHVEGFAKECAVVTHYRLKNDAS
                     GMGIVVDEDARLEEELIVRPTSETIIWNTYKSWIQSYRDLPILINQWANVVRWEMRTR
                     LFLRTTEFLWQEGHTAHATRQEAVEEAERMINVYAQFAREWMAMPVIMGRKSEAERFA
                     GAVDTYSIEALMQDGKALQAGTSHFLGQNFAKAFDVKFLNSEGALDYVWATSWGVSTR
                     LIGALIMAHSDDNGLVLPPKLAPTQVVFVPIYKSREELDRISSVVSQLRNELHAVGVT
                     THYDDRDNYKPGFKFAEYEMKGFPIRIAIGPKDLENGTVEVARRDTLQKQIVSSNSLG
                     KFIIGLLDDIQNNLFSKALEFQRQNTTVVNSYDEFKSVIAGKGGFVLAHWDGTPETEA
                     IIKDETKATIRCIPFDSKPEEGKCIVTGKPSMQRALFAQAY"
     gene            3370..4077
                     /locus_tag="PK502_03635"
     CDS             3370..4077
                     /locus_tag="PK502_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99940.1"
                     /translation="MAEEQQPTKEQIFDLLNGKSVLKQKIYDNTFEVLGKIKLVLRDL
                     ANEYNANLKNTDRRVRLEYRDRGKFEAEIRVAGDLLIFSMHTNVFEFDRDHAIWKLSY
                     VKDDKFASYCGVINIYNFLVDSFRYNRIDDLGYLVGRIFINRDFHYFVEGKRQMGFLY
                     NNFGTAVVDMKSLRQIVETAIMYSLEFDLLVPPYDLVKIASVQQMNSKIESSNMQTGK
                     RLGFKFNSDDVLEEENK"
     gene            4106..5737
                     /locus_tag="PK502_03640"
     CDS             4106..5737
                     /locus_tag="PK502_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HPG99941.1"
                     /translation="MNRLCITFFAALMALSSCGTKHNKEVKSEMQKKLDEFAPVELRS
                     DITHLSTREIQMLPYLFDAAQIMDDLFWLQAFGSKEEFLDTITDKATFEFALVNYGPW
                     ERLNDNKPFIQGFGEKPLGAQFYPPDMTIEEFEAFSSPDKTSQYTLIRRNSDGKLESV
                     PYHITYKDRIDKAAELMVQAANLAEDAGLKRYLNLRAKALQTDEYFESDMAWMNMKNN
                     NIDFVVGPIENYEDALFNYKAAYEAYILIKDLEWSQKISHYAALLPKLQESLPVDPAY
                     KKEVPGLDSDLGVYDVVFYAGDCNAGSKTIAINLPNDPKVQLKKGSRKLQLKNAMRYK
                     FDKILLPIGNLLIDEEQREHIKFDAFFENVMFHEVAHGLGIKNRVDGKGGVQESLKEM
                     HSALEEGKADILGLYMVSQLAAMGELPDKDMMDNYVTFVAGIFRSVRFGAASSHGKAN
                     MVCFYYLKERGAFERNPETGTYRVNFDKMTEAMNSLAHDILILQGNGDYEGVKKLFAD
                     KGFIAEELQHDLDRVSEASIPRDIRCIQGKEIFGL"
     gene            5836..6405
                     /locus_tag="PK502_03645"
     CDS             5836..6405
                     /locus_tag="PK502_03645"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HPG99942.1"
                     /translation="MNLTFNFIKSVSNIYDEMIDNGFSLVYLGEFSHEITKMFTSMAE
                     SDMEKNSEERSVQRKVYHVMVETLQNMNKHSDEIKERNIGNGLFIIGKKHDTYYVITS
                     NKVARKHKDHLEQSLITVNNASPEELKEMYKRQIREGTLSEKGGAGLGLIDIARKTGE
                     PLNYQFLQLDEDNYFFILKVEINARKFYK"
     gene            6446..8350
                     /locus_tag="PK502_03650"
     CDS             6446..8350
                     /locus_tag="PK502_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99943.1"
                     /translation="MSLKKSFLQLTLRLGLAITVHLSFLLFTSTSFALTTSKFDSVQL
                     FWNPDTLSRSDTNLFNRRGNEVLKSNNAIVNNLLDLIYCEEEDTRQKEIYAEQAKAYQ
                     KFSRYAGRIIADIQIVRLKPFGEYINDTTLKPENSLQKFGNSLHSLTLRRSVENNLLF
                     SEGQELDPVDLVETEQLLRNLLYIEDVGINIEPLQDTNYVKATVVTKDKWTLNMGVRI
                     ESSAKGRITLSERNFAGIGIGATGRVYYDEEQKEKWGYMGELNIPNIAGTFITSDLWF
                     REGVGYKSYCVDVKRDFYASKTKYAVGGTYVKSQEPYKIFPVDSIVSIDYSLMSFWVG
                     RSFRVGKPVIIASPYIFTLALKYRQVDFYKSLPTSMNLNPYFHSNYQVLLAAGFSNQN
                     FAQDNFIYSFGATEDIPVGFIVQLIGGVEDSEYQRRLVAGGEISAAEITPAGYLFIAA
                     RTGGYMAEGPRAEQGMINFKSTFISNKFTLGKYFGRQHVGVSFTRGFSRFEGEREYLA
                     LNKDNGITGLRSNSLVGQTRLIVNFETTLFSPHLYYGFRPSCFAFCDLGMVGGSNEVI
                     FGNSLFSGFGLGFKVKNESLIFPSFIFRLGYYPNVPQNADVAYWIITSQDRKKFEQFR
                     MKEPALLPFE"
     gene            8509..9144
                     /locus_tag="PK502_03655"
     CDS             8509..9144
                     /locus_tag="PK502_03655"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPG99944.1"
                     /translation="MGVLVGQKAPVFKASAVIKGHEIVENFSLEQFIGKKYVVFFFYP
                     ADFTFVCPTELLAFQDKLEEFEKRNTQVVACSVDSEFSHWKWLQTEQKDGGIKGVTYP
                     IVSDLSKTISENFDVLAGHYDYDDNNGDAVFVGVPQAFRGLFLIDKSGVVRHQVVNDM
                     PLGRNVDEVIRMVDALQYFEEHGEVCPANWKKGEKAMKATQDGVSDYLSHR"
     gene            9239..10567
                     /locus_tag="PK502_03660"
     CDS             9239..10567
                     /locus_tag="PK502_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HPG99945.1"
                     /translation="MRKRLTYLVENPEVVTDNMAKALLSWDVGIVLRGKDAMPVPGTD
                     MVSQFDCAAGFGVAASCKPADNFFKNFEYFTDANNDWILGFLGYDLKNELENLQSGNF
                     DGLAFPEMFFFVPEVFMSIADHNLCVYYYPGKTSDLTVSKLVDDLLRVELVANPQQPT
                     ITFKSRYSRYEYLTQVQRIKNHIGRGDIYEMNFCQEFYTEGVSIQPARVFNRLYSNSP
                     TPFGAFLKIGEKFLMAASPERYLRKNGNVVVSQPIKGTAPRMALPDMDSQMVEKLKSD
                     AKEISENVMIVDLVRNDLSRVAVPGSVKVNELCGIYTFRQVHQMISTVSCIVPEKLNL
                     VEPIKATFPMGSMTGAPKVRAMQLIEEFEKSRRGIFSGAVGYITPNKNFDFNVVIRSL
                     LYCGQRKYLSFSVGGAITAKSDPENEYNECLLKAKAILQTLNARIENVAG"
     gene            10554..11873
                     /gene="tilS"
                     /locus_tag="PK502_03665"
     CDS             10554..11873
                     /gene="tilS"
                     /locus_tag="PK502_03665"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPG99946.1"
                     /translation="MLQDKFINFIRVHRLADENDSILLGVSGGIDSMVMLHLFQKTGF
                     KFAVAHCNFGLRGEESDGDEEFTRTYCREKGVVFHTTKFSTVQYATDKSVSIQVAARE
                     LRYDYFNELCREHGYNKIAIAHNMNDSAETVLLNLIRGAGIRGLTGIRPVHGNIIRPL
                     LFATRPEIENYSKQLGISYREDSSNATLKYKRNFIRHRVIPLLQELNPSAVETIYDSS
                     YHVNEAISLVDRQLDEIRRKVVSFRKDAVLFSVEELKREPAARIFLVEELRHYGFSGN
                     MATQVFDLLESTPGKYVDSPTHTLLRDRKYLILSAKISTSNACIEVGEDCSRIEKPFP
                     MRFERIVEVKIDSSPQVAILNYSKLRFPLTLRCWQPGDRFIPLGMKGYKKVSDYLVDC
                     KVPLNQKNEVYVLLSNNEIAWVVGYRIDDRYKISTETAEALKISVLL"
     gene            complement(11965..12420)
                     /locus_tag="PK502_03670"
     CDS             complement(11965..12420)
                     /locus_tag="PK502_03670"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99947.1"
                     /translation="MKRLTLLLLLVPFLSGAQNNVTEKNGLYLDSNGKPFTGTYQEYF
                     ENGQVKSQMEIVQGLLNGQVKVYFPSGELNEIRSYIKGEKSGRWETWNAQGVKVAEAN
                     FLNGKKNGKWYVWDEKGTLRYDMTYRMGEKSGTWIIYNDKGLETSRKEY"
     gene            complement(12898..14250)
                     /locus_tag="PK502_03675"
     CDS             complement(12898..14250)
                     /locus_tag="PK502_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99948.1"
                     /translation="MSLLTSTKGLAFAAILLGLGMASCNDDEDEPKPPTGQTVTVSGD
                     ITANTTWSANDTIMLEGFVYVNNGVTLTIEPGALIKGLSDTKAALIVERGGKIMANGT
                     QDRPVVFTSDKPAGQRKPGDWAGIIICGKAPINITGGEAEIEGGTGAIYGGTDPADNS
                     GSLQYVRIEFAGYEVSSGNEINGLTLGGVGSGTVIEHVQVSFGRDDSFEWFGGTVNAK
                     YLVSYRGGDDDFDTDNGFSGNVQYGLILRGPEEADKSDASNGFESDNDANGSSNTPLT
                     SAKFSNITILGPYATKTQPNVNPNHKHALRLRRNTRLNLYNSILAGFATGPRIEGPSM
                     TQYNTTGGPEIKNCIMAGMIKDFQVNTDGTTLTQEQYDAMWTAGNNQTFAENADIKLT
                     NAWSWGNVNPMPQSGSPVLTGASFSGLSGFETVAFIGGFGTTNWTSGWCSWDPQNARY
                     "
     gene            complement(14452..14817)
                     /locus_tag="PK502_03680"
     CDS             complement(14452..14817)
                     /locus_tag="PK502_03680"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPG99949.1"
                     /translation="MKRILVLLIAALTLNVFGATTDDKTKGESTTSLPVKSITINGKV
                     TDKHTGEALAGVAVKTDGSTTCYSDFDGNFSIQVNPEKLENLSAELISYSTSVVKVDI
                     NKTINPTLKIKIELEPIAE"
     gene            complement(14927..17740)
                     /locus_tag="PK502_03685"
     CDS             complement(14927..17740)
                     /locus_tag="PK502_03685"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPG99950.1"
                     /translation="MRTNNRRIAYLFTLLCFLLATSASAQTGSIVGTVVDMGKDETLV
                     GASVIIEGTFKGTITDFDGKFKLENLEPGIYSVQASFISYKPTILDGIRVEANKTTTV
                     SIELEEITTELEGVTVTATRKTSTNLAMISSIKASLMVATGISAQQINNSQDKDASEV
                     IRRVPGASIIDDRFVVVRGLNQRYNSVWINNASTPSSETDQKAFSFDVIPSAMIDNMM
                     VSKSMSPELPADFTGGFIKIFTKNMPESNFMSISYTTGYNTQAHYGDFQLITTGKTDW
                     LGVDGGTRNLPSGFPNSLKGYPDAELADLGKRLNTSWAPKGVSAFPEQKLNFSFGRKI
                     QKGNRLLGNITSISYSHTYSSDELVNKGYQVQLNPGEIPPYSYNYTDSTYSRSVRLGI
                     LHNWSLFPGKGTKIEFRNLFNLMGNNTAVIRNGLNGYEGYTIRSYQDKYMSRITYSGQ
                     LGGEHKLGSNSDNTLDWVLGFAYSGRNEPDLKQLRTTLQSEPLLPHYNEYYASVGLTP
                     SVSDAGRLFMNLHEYIATAGTNYEAKIKIGSWKPVIKTGLYSEYKNRTFDARILGFAK
                     NVSYTESVWLPVETIFSLENINTTPSGFILKEMTSRSDSYDATNLLLAGYLTINTNIT
                     QKLRFYGGARFEMNNMRLNSYDMYNKPVDVKLDHIDIFPSANLTYNFNEKNLLRLAGG
                     LSVNRPEFREIAPFYFYNFDEEADYVGNVNLKNAYVWNFDFRYELYPNAGEIISIGIF
                     YKHFTSSIESVFRPAGNRLTFTYDNADNAQSAGAEVELRKSLEFIPLLKDFSAVLNAS
                     YIYSRVNFPANSIFRDRPMQGQSPYLVNAAVFYSNTKHKLNLSLQYNLIGDRILAVGL
                     PFQNSNQDIPDITEKHSHLVDFTASKGIGSKMEIKAGIKNLLNQKSIKYQAFKQADGG
                     DLNLSSREMQPGITFSLGFSLNF"
     gene            17931..18623
                     /locus_tag="PK502_03690"
     CDS             17931..18623
                     /locus_tag="PK502_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPG99951.1"
                     /translation="MSKPGYRILIVDDEPDIIEFLSYNLTKEGYEVDTATNGRRAIEK
                     ATEFSPDLILLDVMMPDMDGIETCQELRANPLTKDCLIAFLTARSEDYSQIAGFDAGG
                     DDYISKPIKPKVLISRIKALLKRSGSLEEAPEPAPEIPEEGIYINRERYLVYLDGREL
                     VLPKKEFELLALLQSKPQKVFTRDEIYQAIWGDDIVVGDRTIDVHIRKLREKLGDQYI
                     RTVKGVGYKFTV"
     gene            18689..19096
                     /locus_tag="PK502_03695"
     CDS             18689..19096
                     /locus_tag="PK502_03695"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HPG99952.1"
                     /translation="MELFSWKNDYSVKVGSIDSDHQKLFDIINSLFTAMSKGEGRQVI
                     SGLVKELQSYTIYHFNREETFFRVTSYPNAANHLEQHKIFVSKVKEYKEKIDKGNNNF
                     SPEMLTYLKDWLLNHILVTDKAYSDHFTKNGIV"
     gene            19322..19729
                     /locus_tag="PK502_03700"
     CDS             19322..19729
                     /locus_tag="PK502_03700"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HPG99953.1"
                     /translation="MELISWESNYSVKVGSIDADHKKLVEILNSLFMAMSKGEGSKII
                     SDLVKELQRYTIYHFSREESFFRITGYPNTQKHIEEHKQFIGKVKEYKEKIDKGNNNF
                     SSEMLAYLKDWLLNHILVTDKAYSEHFVKHGIA"
     gene            complement(19829..21442)
                     /locus_tag="PK502_03705"
     CDS             complement(19829..21442)
                     /locus_tag="PK502_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HPG99954.1"
                     /translation="MQKASTSTTRIFLFILGFTYLHPTFSAAQQKTTIKLEGAKAMKN
                     LNLGDQKIVRYIGNVRFFYDNTRVRCDSAYYSQNENWFDAFNGVVVSQGSSNIYGDKL
                     HYDGRTSEGRLTGKEVKMVDKDFTLVTDALDFNSKTSLAFYTTGGVATSNDSSTLTSI
                     KGYYNNNDNLISFANKVVLKSPDGNAFTDSLSYHTKNEVAYFFGPTFIYDGENFIYCE
                     KGWHNRKLQQSNFQNNAYLISNSQKLFGENIYYDKQNGYARGIGNVVMVDTVNRTYIY
                     GQKASYWQEKEEAEITEQPYMMMIDDSDSLFLRTDKLLMITAKTGDNQKTDSSYRLVK
                     AIGRVMYYRHDIQGVCDSMIYNTRDSVLEMYGNPVMWNEANQMTADFIRIHISNKKIS
                     QADFLGNAFISSREDSAYFSQMRGKTILARFSDGQLTRMDITGNGQVVYFVRDKGQIT
                     MVNRAEGSNVDVHIKNNKVSRVVFKQKPTSNLIPIEQVEPEDMTLKGFKWLGQLRPES
                     KYSVIPTSLTLVPLEKGTQRRMEMVANFK"
     gene            complement(21455..>22082)
                     /locus_tag="PK502_03710"
     CDS             complement(21455..>22082)
                     /locus_tag="PK502_03710"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPG99955.1"
                     /translation="KAFADAAFSLKEGQISQIVETEYGFHIIQLIEKRDNQVNVRHIL
                     MKPQFSGDMLAQAMSKLDSIATLIKADSLTFEAAAMRFSQDKKSFLNGGLVINSYNNT
                     SLFEKEQLQPADFYAIRELKEGEMSSPFESRDEHANVVFKIIKVKRIIPPHKANLKED
                     YAIIQNIAKVSMENEILQEWLDKKIQSTYIRVDEPFRNCSFRSKGWIK"
BASE COUNT         5915 a         4580 c         5163 g         6424 t
ORIGIN      
        1 cttttggttt ttaaatgaag gggtaacggt tagctctcct gacttgtaaa cacccaatcc
       61 tgccggattg atgcttaggg aggtgaaatc gccccccaag gcaccaatgg ctcctcccag
      121 cgaaatgctg cgggctgttc ctccattaag ggtttgcgaa aatcgaattc catcctctat
      181 ggtttgtgaa aaaagttgag tgacagtacc tgaagccatt aataccagaa aaaatctcga
      241 aatatgcctt atcattgtaa taggtttatg tagatgctaa tgattgtttt ttcaccacag
      301 tgtaaaaatt gctgagtaaa gggaggaatt tagttattcc tccctgctac acagctaaga
      361 gatttttatc ggccacgtgt tttggctcca gaggatcccg agttgctatt tcctcttgaa
      421 cctgatgacg agctttcagt tcttggagtg taagttccgc tggaatttcc cgaagacctg
      481 gaaccctcag gttttgactg caccgaattt cctgatcgtg ttgctgagcc atttgaaggt
      541 gctgaaaccc tgccggggct attgtatgtg ggagaggaag acgatctacc cgatggtgat
      601 gtggttgtac ctctgccagt gcttggacgc tggatagact gtgttccgga tgagctggtt
      661 cggggtttat cgtagccagg gttgtatgct ctcccggttg gctctgatat tccaatggag
      721 cgcgtaggcc tttcgctccg cgttccagaa cttacagctg aacctcttgt agatgaaggg
      781 tttgatagag tagtgccggt ttctctgcga ggagtagtaa tttggtcacc cgaacgttga
      841 gtaccttctg cacttcttct gttgttttca taggtgggtg ttacgcgtgt ttgggtgttt
      901 ttggtagatt gaataggatt tatggagctg ctttgcacgt cacctctctg gcgggtaaca
      961 gcctggctgc tagttggcgt aactcctgaa gctgttcggc gggaagaagt gttgaaggat
     1021 gattcacccg gccggcgacc atagtagaag tttccgctgt taaggtagct gtattcaata
     1081 ccgtggttta tccagtagtt tcctgggaaa ttccatgctg agtagtagta agggttgtag
     1141 tagttgaatg ggctatagaa gaaggggtcg tagtaccacg accatggatt atagccccat
     1201 ccatacccga agccgaaata gaatgaggtt ctgggccacg agaaggagtt gtagatgtac
     1261 cagggttcaa cccaaacctg actacccatt accactaacc tgtagtagtt ggggtcatag
     1321 gcctgagcgt agcggtagct atccgagtag tacaccgacc agtcgttcag cccgtacata
     1381 gggtcttcca ttccctttaa cctacgttca tacgactcct gataggaatc ggagagaacg
     1441 ctttcgtatg ggttgacgtt ttccttgtcg gatgaaatgg tgtcgttttg ggtggtttcc
     1501 tggtttacgg ccagctcgtc ggcatacttt ttgtccagct gggcgtaatc atttaaatta
     1561 gtttgaacag gagtgctatc cttactatcc tgggcaagtt ctgttttgga agttttggtg
     1621 tttgccctgt aaatctcatc ctcccacgat gaggtattgc taacctgcag gctcgacgag
     1681 caggacagcg tagtaaatcc cagtagggca agcattggaa tatagctttt tgttttcatg
     1741 gcgcacctcc tgtttgaaaa ttatgttatt attatggtta cttttgctag ctgaaaaatc
     1801 atctaaatat caacaaaaat cataccaata gaacgatatg gcaaaggaaa ttactagtag
     1861 aaacgagaat tattctcaat ggtataacga tatagtgatt aaggctgggt tggccgacta
     1921 ctcggcagtt aggggttgta tgattattaa accttacggc tacgctgttt gggaaaaaat
     1981 tcaggcacaa ctcgacagga tgtttaagga aaccggccat cagaatgcct acttcccact
     2041 tttcatcccc aagtcgtttt ttagccgtga ggcatcgcac gttgagggtt ttgccaagga
     2101 gtgtgctgtg gttacacatt accgcttaaa aaatgatgca agcggtatgg gcatagtggt
     2161 tgacgaggat gccaggctgg aggaggagct gatagttagg ccaacatccg aaaccattat
     2221 ttggaacacc tacaagtcgt ggattcaatc gtacagagat ctgccaatct tgataaacca
     2281 gtgggctaac gtggtgcggt gggagatgcg tacaaggcta ttccttcgta ccaccgaatt
     2341 tctttggcag gaggggcata cagctcatgc cacccgacag gaggcagtag aagaagcgga
     2401 aaggatgatt aacgtgtatg cccaatttgc acgtgagtgg atggccatgc cggttatcat
     2461 gggtcggaaa tcggaagccg agcggtttgc cggagccgtt gacacctact caatagaggc
     2521 tttaatgcag gatggaaaag ccttacaagc cggtacatct cacttcctgg ggcagaattt
     2581 tgccaaagct ttcgatgtta agttccttaa cagcgaggga gcgcttgatt acgtttgggc
     2641 tacctcgtgg ggagtttcca cccggcttat tggtgcgcta atcatggccc actccgacga
     2701 taacgggctt gtacttcctc caaaactggc tcctactcag gttgtctttg tcccaatcta
     2761 taaaagcagg gaggaacttg atagaattag cagtgttgtg tcgcagctta gaaatgaact
     2821 tcatgcagtg ggggttacta cccactacga tgatagggat aactataagc ctggattcaa
     2881 gtttgccgaa tatgaaatga agggtttccc cattagaatt gccattggcc caaaggattt
     2941 ggaaaatgga actgtagagg ttgcccgcag ggatacactt caaaaacaaa tcgttagcag
     3001 caattcatta ggaaagttta ttatagggtt gcttgatgat atccagaata acctgtttag
     3061 taaagcgctt gaatttcaaa ggcaaaacac taccgttgtc aacagctatg atgagtttaa
     3121 gagtgtaatt gcaggtaagg ggggatttgt tctggctcac tgggatggaa ctcccgaaac
     3181 agaagccatt attaaggacg aaacgaaggc aaccatacgc tgcatcccct tcgacagcaa
     3241 gcccgaggag ggaaagtgta ttgtaaccgg taaaccctcc atgcaaaggg ctctttttgc
     3301 ccaggcatac tagggttgaa ttatttaaaa tgcttttgta tatttgattg tttttttaat
     3361 acatggggta tggccgaaga acagcaacca accaaggagc aaatattcga tttactaaac
     3421 ggtaagtcgg ttttaaagca aaaaatctat gacaacacct ttgaggtgtt gggtaaaatt
     3481 aagcttgtgc ttcgcgattt ggccaatgag tacaacgcca accttaaaaa tacggacagg
     3541 cgggttaggc tcgagtaccg cgatagaggt aagtttgagg cagaaatccg ggtggcaggc
     3601 gatcttctca ttttcagcat gcacaccaac gtctttgagt tcgatagaga ccacgccatc
     3661 tggaagcttt cctacgtgaa ggatgacaag tttgcctcct actgtggcgt tatcaatatc
     3721 tacaactttt tagttgactc ttttagatac aacagaattg acgatttggg ctaccttgtt
     3781 ggccgaattt ttatcaacag ggattttcac tactttgtag aggggaaacg gcaaatgggt
     3841 ttcctttaca ataactttgg aactgccgta gttgacatga aatcgcttcg acaaatcgtt
     3901 gaaaccgcca ttatgtactc gctggaattc gatttgttgg taccacccta cgacctggtt
     3961 aaaattgcat cggttcagca gatgaacagc aagattgaaa gttcgaacat gcaaaccggc
     4021 aaacgtttgg gattcaagtt taactccgac gatgtgttgg aggaagaaaa caagtaggct
     4081 ataaaccagc taatttacaa ccaccatgaa cagattgtgt attacgtttt ttgccgcact
     4141 gatggctctc tcatcctgtg gtacaaaaca caacaaggag gttaaatccg agatgcagaa
     4201 aaaattggac gaatttgcgc ctgttgagct gaggtcggac atcactcatt tgtcaactcg
     4261 cgaaattcaa atgctgccct acctatttga tgctgctcag ataatggacg atttattctg
     4321 gttgcaggca tttggaagta aggaagagtt tctcgacaca attaccgata aggccacctt
     4381 tgaatttgcc ctggttaact atgggccgtg ggagcggttg aacgacaata agccctttat
     4441 tcaaggtttt ggcgaaaaac ccctaggcgc ccaattttat cctcccgaca tgaccattga
     4501 ggagttcgaa gcctttagct ctcccgacaa aaccagccag tacaccctca tcaggcgcaa
     4561 ctctgatggg aagcttgaat cggttcccta ccatattact tataaggata ggatagacaa
     4621 ggcggctgag ctaatggtgc aggcagccaa cttagccgag gatgcaggct tgaaacgata
     4681 tttaaatctt agagctaaag cgctgcaaac cgacgagtac tttgaaagcg atatggcctg
     4741 gatgaacatg aaaaataaca acatagattt tgttgttggg ccaattgaaa actacgagga
     4801 cgcactgttc aactacaagg ccgcctacga agcctacatc ctgattaagg atcttgaatg
     4861 gtcacaaaaa attagccatt acgccgctct gcttcccaag ctgcaggaga gcctgccggt
     4921 tgatcctgca tataagaagg aagttcctgg gcttgattcc gatttgggcg tgtacgatgt
     4981 tgtgttctat gcaggcgact gcaatgcagg aagcaaaaca attgccatta acctaccaaa
     5041 cgatccaaaa gttcagctca agaagggtag ccgaaagctg cagctgaaaa atgccatgag
     5101 gtacaagttc gataagattc tcctgcccat tggcaacttg ctgatagacg aggagcaacg
     5161 cgagcatatc aaatttgatg ctttttttga gaacgtaatg tttcatgagg ttgcccacgg
     5221 cttgggaatc aaaaaccggg ttgatgggaa aggtggtgtg caggaatcgc ttaaggagat
     5281 gcacagcgca ctggaagagg ggaaggccga tatacttggt ctttacatgg tgtcgcagct
     5341 ggctgccatg ggagaactcc cggataagga catgatggac aactatgtaa cctttgttgc
     5401 cggtattttc cgatctgtac gttttggagc tgcctcttcg catggaaaag cgaacatggt
     5461 ttgcttttac tacctcaagg agcgtggtgc ctttgagcga aatcccgaaa ccggaaccta
     5521 ccgggttaac ttcgataaaa tgaccgaagc aatgaactcc cttgcccacg atatcctaat
     5581 acttcagggg aacggggatt atgagggtgt taaaaaactt tttgccgata agggatttat
     5641 agccgaggag ctacagcatg acctggatcg ggtttcggag gctagcattc cacgagacat
     5701 tagatgtatt caaggcaaag aaatatttgg cttatagctt gtaaaggtcg ggttctccga
     5761 cctttttttt gagttcatat tgaaatattt taattttaac ggctacattt atccaaattt
     5821 tggagaccta aagctatgaa cctaaccttc aactttatca agtcggtgtc gaacatctac
     5881 gatgagatga ttgataacgg gttttcactg gtgtatctgg gggaatttag ccatgaaata
     5941 accaagatgt ttacctccat ggccgaatcg gatatggaga agaacagcga ggaaagatcg
     6001 gttcagcgta aggtgtacca tgttatggtt gaaacccttc agaatatgaa caagcattcc
     6061 gatgaaatca aagagcgaaa cattggcaac gggctcttca tcatcgggaa aaaacatgac
     6121 acctactacg tgattacctc caataaggta gctcgcaagc ataaagacca ccttgagcaa
     6181 tcgctgatca ctgttaataa cgcttcgccc gaagagttaa aagaaatgta taaacggcaa
     6241 attcgcgaag gaacgctatc cgaaaaggga ggtgccgggt taggactaat tgatattgcc
     6301 cgcaaaacag gcgaaccatt gaactaccaa ttccttcaac tcgatgagga taactatttt
     6361 tttatactta aagtcgaaat taatgcccgt aagttctaca agtaagtggt taaaggagta
     6421 atcctatgtc ttattaatgg gtattttgtc gcttaaaaaa agctttttac agctaacctt
     6481 acgcttagga ttagcaataa cggttcattt atcatttctg cttttcacct ccacttcttt
     6541 tgcattaaca accagcaagt ttgattctgt tcagctattt tggaatcccg acacccttag
     6601 tagaagcgac accaacttgt tcaatcgtcg agggaatgag gttcttaagt ccaataatgc
     6661 catagtaaat aacctgttag acctaattta ctgtgaggaa gaggatacta ggcaaaaaga
     6721 aatttatgcc gaacaggcaa aggcatacca gaagttctca agatatgctg gaagaataat
     6781 agccgatatt caaattgtac ggttaaagcc ttttggggag tacattaacg atactacact
     6841 aaaacccgaa aacagccttc agaaatttgg gaactcgcta cactcgctta cattacgccg
     6901 gtcggttgaa aacaacctgt tattttccga ggggcaagaa cttgacccgg ttgacttagt
     6961 ggaaaccgaa cagctccttc gcaaccttct ctatattgaa gatgtaggta ttaacattga
     7021 acccctgcag gacactaact atgtgaaagc tacggttgtt acgaaggata agtggactct
     7081 gaacatgggg gtacggattg aaagttcagc gaagggacgg ataacccttt cggagagaaa
     7141 ctttgccgga ataggtatag gggcaaccgg gcgtgtttac tacgacgagg aacaaaagga
     7201 gaaatggggg tacatgggtg agctgaatat ccctaacatt gctgggactt tcattaccag
     7261 cgacttgtgg ttccgtgaag gcgtcggcta caaatcttac tgtgtagatg tgaagcgtga
     7321 cttttacgcc tcaaaaacca agtatgcagt aggcggaacc tatgttaaaa gtcaagaacc
     7381 ctacaaaatt tttcctgttg attcaatagt ttcgatagac tatagcctaa tgagcttttg
     7441 ggtaggccgt tcatttaggg ttggtaaacc ggttataatt gcttcgcctt acatttttac
     7501 ccttgctcta aagtaccggc aggttgattt ctacaaatcc ctaccaacat ctatgaacct
     7561 aaacccgtac tttcactcta actaccaagt tttgttagca gccggatttt ctaaccaaaa
     7621 ttttgctcag gataacttca tctactcttt tggcgcaacc gaggatattc cggttggatt
     7681 tatagtgcag cttattggag gggttgagga tagcgagtac cagcgacgat tggtggcagg
     7741 tggtgaaatt tcggctgccg agataacacc tgccggatac ctttttattg ctgctcgtac
     7801 cggaggctac atggccgaag gcccacgagc cgagcaagga atgatcaact tcaaatctac
     7861 ttttatttct aataaattta cccttggaaa atattttggc cggcaacatg ttggtgttag
     7921 ctttacaagg ggcttttcgc ggtttgaggg ggaaagggaa taccttgcac ttaacaagga
     7981 taacgggatt accggtctaa gaagcaattc ccttgtgggt caaacacgcc taatagttaa
     8041 ttttgaaaca actttgttta gcccacattt atactatggt tttagaccat cgtgttttgc
     8101 cttctgtgat ttgggaatgg tgggaggttc aaacgaagta atttttggca attctctttt
     8161 tagcggtttc ggtctgggtt ttaaggtgaa aaatgaaagt ttaatctttc cgtcatttat
     8221 ttttcgtctt ggttactacc ctaatgtccc ccaaaatgcc gatgttgcct attggattat
     8281 tacctctcag gatcgtaaga agtttgagca gttcaggatg aaagagcctg ccctacttcc
     8341 cttcgagtag cctggcatct gcgaacaatt ttcatttcac gttgtttacc atgaaaattt
     8401 acaacccggc ataattgaac atttctggtt aattgcatgg ttgtattaag tggcagctaa
     8461 agtcattttt gattttacat tattaaaata cctaaaaatt attgaactat gggagtacta
     8521 gtaggacaga aagcgcctgt tttcaaggct agcgcagtta taaagggtca cgaaattgtt
     8581 gagaattttt ctcttgagca gttcatcggc aagaagtatg tggtattctt tttctaccct
     8641 gccgatttta cttttgtttg cccaacagaa cttttagcat ttcaggataa gctggaggag
     8701 tttgaaaaaa gaaacacaca ggtggttgca tgctcggttg attcggagtt ttcgcactgg
     8761 aaatggctgc aaaccgaaca aaaagatggc ggcattaagg gggttaccta cccaattgtt
     8821 tcggatttat ccaaaacaat ctccgagaat tttgatgtgc tggccggtca ttacgactac
     8881 gatgacaata acggagatgc tgtatttgtt ggcgttccac aggcattcag gggattgttt
     8941 ctgattgaca agtccggggt tgtccgccat caggtggtta acgatatgcc tctgggaagg
     9001 aatgtggacg aggtaatccg tatggttgac gcacttcagt atttcgagga gcatggcgag
     9061 gtttgccctg ccaactggaa aaaaggtgaa aaggccatga aagcaaccca ggacggcgtt
     9121 tcggactacc taagccatag gtagtaagcc tacttgcata tccacttaac ttgctgaacc
     9181 gcctctaggg gcggtttttt tgtgctaaat ccttcatctt taatgctaat tttgtggcat
     9241 gaggaaacgg ttaacatacc ttgtcgaaaa ccctgaagtg gttaccgaca acatggctaa
     9301 agccctgcta agctgggatg ttggcattgt tttaagagga aaggatgcta tgccggttcc
     9361 gggaacagac atggtaagcc agttcgattg tgcagcagga tttggtgtgg ctgccagctg
     9421 caaacctgcg gataattttt ttaaaaactt cgaatacttt accgatgcta ataacgattg
     9481 gattttaggt tttttaggtt acgaccttaa aaatgagctg gagaatcttc aatcggggaa
     9541 tttcgatggg ctggcatttc ccgaaatgtt cttcttcgtt ccggaagtat ttatgagtat
     9601 tgctgaccac aatctttgcg tgtactacta tcccggtaaa acctctgacc taactgttag
     9661 caagcttgtt gacgacctgc taagagttga attggttgct aaccctcaac agcctaccat
     9721 cacttttaag tccagatact cgagatatga ataccttacc caggttcagc gcattaagaa
     9781 ccatatcggc aggggtgata tttacgagat gaatttttgc caggagtttt acaccgaagg
     9841 ggtatcaatt cagcctgccc gggtgtttaa ccgcttatat agcaactcac ctacaccgtt
     9901 tggcgcattt ttaaaaattg gcgaaaagtt tttaatggca gcttctcctg aacgttacct
     9961 taggaaaaac gggaatgtag tggtaagcca gccaattaaa ggcactgcgc caagaatggc
    10021 tttgccagac atggattcgc agatggtgga aaagctaaaa tcggacgcca aggagatatc
    10081 cgagaatgtg atgatagttg atttggtccg aaatgacctg tcgagggttg cggttcccgg
    10141 ttcagttaag gttaacgagc tctgcggaat ctacaccttc cggcaggttc accagatgat
    10201 ttccaccgtt tcgtgcatcg tgcctgagaa gctaaacctg gtagaaccca taaaagccac
    10261 attccctatg ggatctatga ccggtgcccc aaaggtaagg gcaatgcagc tgattgaaga
    10321 gtttgaaaaa tccaggaggg gaattttttc aggcgcagtg ggctacatca ctcctaacaa
    10381 aaattttgat ttcaacgtgg ttatccgtag cttgctttac tgcgggcaaa ggaagtacct
    10441 ttccttttcg gtgggaggtg caattaccgc caagtccgac cctgaaaatg aatacaacga
    10501 atgcttgcta aaagccaaag ccatacttca aacgttaaac gccaggattg aaaatgttgc
    10561 aggataaatt tatcaatttc attcgggtac accggttggc cgacgagaac gactccatcc
    10621 tgctgggtgt tagcggtggc attgattcca tggtaatgct gcatcttttt caaaagactg
    10681 gtttcaaatt tgccgtggcc cactgcaatt ttgggttaag gggtgaggag tctgatggcg
    10741 acgaggagtt tacccgaacc tactgccgtg aaaaaggagt ggtgttccac actactaaat
    10801 ttagtacggt tcaatacgct actgataaga gtgtttccat tcaggtggct gccagggagt
    10861 tgaggtatga ctacttcaac gagctttgtc gggagcacgg ctacaacaaa attgccattg
    10921 cccataacat gaacgacagc gcagaaacgg ttttgctcaa cctaatccga ggggcaggta
    10981 ttagagggtt aacaggtatc aggcctgttc atggaaatat aattcgaccg ttgctctttg
    11041 ccactcgccc tgagattgaa aactactcaa agcagctggg aattagctac cgtgaggatt
    11101 ccagcaacgc aaccctaaag tacaaacgaa atttcataag gcatagggtc attccgctgc
    11161 tacaagagtt aaacccttca gcggtggaaa caatttacga ttcgtcttat catgtcaatg
    11221 aggctatatc gctggttgac aggcagctgg acgagataag aaggaaagtt gtttcattca
    11281 gaaaggatgc tgtgctattt tcagttgaag aactaaagcg tgaaccggca gcccgtatat
    11341 ttctggttga ggagctacgg cattacgggt tttcgggaaa tatggcaaca caagtctttg
    11401 acctgctgga gtcaactcca ggaaagtatg ttgactcgcc gactcatacc ctgctaaggg
    11461 atagaaaata cctgattctg tcggctaaaa tttctacaag taatgcctgc atagaggttg
    11521 gcgaagattg cagccggatt gagaaaccct ttcccatgag gtttgagcgt attgttgagg
    11581 ttaagattga ttcaagtccg caagtagcca tccttaacta ctctaagcta aggtttccgc
    11641 ttacacttcg atgctggcaa cctggcgata ggtttatacc tttaggcatg aaaggctata
    11701 aaaaggtaag cgattacctt gttgactgta aggttccttt aaaccaaaaa aacgaggtgt
    11761 atgttctgct ttccaataat gaaattgcct gggtggttgg ctacaggatt gacgacaggt
    11821 ataaaatatc cactgaaaca gctgaggcct tgaaaatttc ggtgcttttg tagcaataca
    11881 acggcagaaa tgcaaacaca accacccccc tttaaggtag cggtacctga ttacgtgaag
    11941 tttaaccttt tgaagagtgg gagactagta ttcttttcgg gaagtttcta agcctttatc
    12001 gttgtaaatt atccatgttc cggatttctc ccccatccgg taggtcatat cgtagcgtaa
    12061 agtccccttt tcgtcccaca cataccattt accatttttc tttccgttga gaaagttggc
    12121 ttcagcaact tttaccccct gagcattcca tgtttcccat cttccgcttt tttccccttt
    12181 tatgtacgac cgaatttcat tcagctcacc cgacggaaaa tagactttta cctgcccgtt
    12241 gagtaagccc tggacgattt ccatttgaga ttttacctgc ccgttttcaa aatactcctg
    12301 atatgtgcca gtgaagggtt taccattgct gtcaaggtat agcccattct tttctgtaac
    12361 attattttgg gcaccggaca gaaaaggaac taaaagtaaa agtaacgtaa gtcttttcat
    12421 attatggtgt ttagcaggtt gatagttaat gtgtaaaata tttttatgct ataaaacagc
    12481 ttagtagaat actcttaatt tttacttgaa acaggggctg gaggtttcat ctgcaaagat
    12541 ttttttggtt tcttattaaa aatactaagg tccataccgt ttaagtatcg gtgttaaagc
    12601 tgatggaaca tccatatttt ggcttcacca atcccgtaag aagcgtacgg ttgttcatgt
    12661 atgcttgtgc atgaagctct acgagggtat ttcataagaa atcattttta tttattattt
    12721 acaggcaaaa agcaaaaggg ttaaaaaggc caggcatgta ctggctaaaa caagttttac
    12781 ctatttgtaa ttttgactaa aaaatcaaat gcagtataaa cgattagaaa ggtagtagta
    12841 tattcaaaaa aaacttctcc cggattaagg gagaagcttt taaggttggt tataacatta
    12901 gtatctagcg ttctgtggat cccagctgca ccacccgctg gtccagtttg tagttccaaa
    12961 accaccaatg aaggcaactg tttcgaagcc agacaacccg ctaaaggaag cgcctgtgag
    13021 aacaggagaa ccactttggg gcataggatt aacatttccc cagctccatg cattggtaag
    13081 ttttatatct gcattttcag caaaggtctg attgttacct gctgtccaca tggcgtcata
    13141 ctgctcctgg gtaagggtag tcccatcggt gttaacctga aaatccttta tcatgccggc
    13201 cattatgcag ttcttaattt caggaccccc tgtagtgttg tattgcgtca tgctaggacc
    13261 ctcaatacga ggaccggtgg caaaacctgc caggatagag ttgtaaaggt taagacgtgt
    13321 attacgacga agacgaaggg catgcttgtg gttggggtta acatttggtt gggtcttagt
    13381 ggcatagggg ccaagaatag tgatgttgct gaactttgca gaggttaatg gagtatttga
    13441 tgagccgttt gcatcgttat cggattcaaa cccgttggat gcatcggact tgtcagcttc
    13501 ctctggacca cgaagtatta aaccatactg aacatttccg ctaaagccat tgtcggtgtc
    13561 aaaatcgtca tcaccaccac ggtacgaaac caggtacttg gcatttacgg ttccgccaaa
    13621 ccactcgaaa gagtcatcgc ggccaaaaga aacctgcacg tgttcaatta cagttccact
    13681 tcctacgccc cccaaggtaa ggccgttaat ttcatttccg ctggaaacct cgtatcctgc
    13741 aaactctatg cgaacatact gaagcgatcc ggagttgtcg gcaggatcag tacccccgta
    13801 aatagcgccg gttcctcctt caatctcagc ctcaccaccg gtaatattaa taggagcctt
    13861 gccacaaatt attattcctg cccagtcgcc aggttttctc tgaccggccg gtttatcgct
    13921 agtaaataca acaggtctat cttgtgttcc attggccata attttaccgc cacgctcaac
    13981 tatcagcgct gccttggtgt cgcttaaacc cttaattagc gctcccggct caatggtaag
    14041 cgtaacccca ttgttcacgt aaacaaaccc ttccagcatg atagtatcgt ttgccgacca
    14101 ggttgtgttg gctgtgatat cgccagaaac cgttaccgtt tgtcctgtgg gaggcttggg
    14161 ttcgtcttca tcatcattac atgatgccat gcccagacca agtaggattg ccgcaaaggc
    14221 taaacccttt gtgcttgtta agagactcat ttttttcatt ttgattgatt ttagaattat
    14281 ctggcacaaa agtagggctg agcagctaaa gcgcagctaa gcaaaaatta ccctttcatt
    14341 agctgtttgt taggtatctg ttaacaggga agtggtagtg tttctagaat gaaaaagcca
    14401 cacctgggtg aaggtgtggc ttagtggtga acttagctac gatgagttac tctactcagc
    14461 aatgggttcc agctcaatct ttatcttcag tgtagggttg atagttttat ttatgtcaac
    14521 ctttacaact gaagtagaat aggagataag ttcggcacta aggttttcga gtttctccgg
    14581 attaacttgt atgctgaagt tcccatcaaa atcggagtag caagttgtgc ttccatcagt
    14641 tttgacggca acccctgcca gtgcctcccc tgtatgcttg tcggtaactt tcccgttaat
    14701 ggtgattgac ttaacgggca agctggttgt gctttcacct tttgttttat catcagtggt
    14761 tgctccaaaa acattcaagg ttaaagcagc tattaacagc actagaattc ttttcatggg
    14821 tatttgtttt acttttgttt ctgaagcaaa ggtagtatgg tggtatttcc ttaccattat
    14881 atcaatatga ttaaaatgtt aagttgatgt taaacaggtt ttggtgctaa aaattcagtg
    14941 aaaagcccag gctaaatgtt atcccgggct gcatttccct actgctcaag tttaaatcac
    15001 ctccatcagc ctgtttaaac gcttggtact tgatgctctt ttgattcaag agatttttga
    15061 tacctgcttt tatttccatt ttgctgccaa ttcccttgct ggcggtgaag tcaaccaggt
    15121 ggctatgttt ttcggtgata tcggggatgt cctggttgga gttttggaat ggtagcccca
    15181 cagctagaat cctatcacct ataagattat actgcaacga tagatttagc ttatgcttgg
    15241 tgttgctgta aaacactgct gcattaacca ggtagggcga ttgtccctgc atgggtctat
    15301 cacgaaatat agagtttgca ggaaagttta cccggctgta tatgtatgag gcattaagca
    15361 cagcgctgaa atcctttagc aggggaataa actccagtga ttttcgtagc tcaacttctg
    15421 ctcctgcgct ttgggcatta tctgcattat cgtaggtaaa tgttaagcgg tttccggcgg
    15481 gtctgaacac cgattcaatt gaggatgtaa agtgcttata gaagattcct atagaaataa
    15541 tttcaccggc attcgggtat agctcgtagc ggaaatcgaa gttccacaca tatgcgtttt
    15601 ttagatttac atttcccacg taatcggctt cctcgtcaaa gttgtagaag taaaatggtg
    15661 caatttccct aaattctggg cgattaacgg atagcccgcc ggctagacgc agcaaatttt
    15721 tctcattgaa gttgtaggta aggttcgccg atgggaaaat atctatgtgg tcgagtttaa
    15781 cgtcaacggg cttgttgtac atgtcgtagc tgttgaggcg catgttgttc atttcgaatc
    15841 tcgctcctcc gtaaaatctc agcttttggg ttatattcgt gttgatggtc aggtatcctg
    15901 caaggagcag gttggtggcg tcgtatgagt ctgatctgga agtcatctcc tttagtataa
    15961 aaccgcttgg ggtagtgttg atgttttcca ggctaaaaat tgtttcaacc ggaagccaga
    16021 ccgattcggt gtaggagaca tttttggcga aacctagtat tctggcgtca aatgttctat
    16081 ttttatattc agagtaaagt ccggttttaa taacaggttt ccagctccca atttttattt
    16141 tagcttcgta gtttgtccct gcagtggcta tgtattcgtg aaggttcatg aatagcctgc
    16201 cggcgtcgct cactgaaggt gttaacccaa ctgaggcata gtattcgttg tagtggggca
    16261 gtagtggctc agactgtagg gtggttcgaa gttgttttaa gtcgggttcg ttacgtcccg
    16321 agtaggcaaa tcctaaaacc cagtcaaggg tattatccga gttgctgccc agcttatgct
    16381 ctccacccag ctgtccggag taggttatcc tgctcatgta tttatcctgg tatgaccgga
    16441 tggtataacc ttcatagcca tttagcccat tccgaataac ggcagtattg tttcccatca
    16501 ggttgaagag atttctaaac tcgatttttg ttccttttcc agggaacagc gaccagttat
    16561 gtagaatccc cagcctgacc gagcgggagt atgtgctgtc ggtatagtta tagctgtatg
    16621 gaggaatttc gcctgggtta agctgcacct ggtaaccttt gtttaccagc tcatcgctac
    16681 tgtaggtatg gctgtagctt atggatgtta tgttgcccag caaacggtta cccttttgaa
    16741 tttttctgcc aaagctgaag ttgagctttt gctcaggaaa agcgcttaca ccttttggtg
    16801 cccagctggt gtttagcctt tttcccaaat cggccagctc agcatcaggg taacctttta
    16861 gggaatttgg aaatcctgag ggtaggtttc gggttccgcc atccacccct aaccaatctg
    16921 ttttcccggt ggttatgagc tgaaaatcgc catagtgtgc ctgggtattg taacccgttg
    16981 tgtagcttat gctcatgaag ttgctttcgg gcatattttt ggtaaaaatt ttaatgaatc
    17041 caccggtaaa atcggccggt agctcaggcg acatgctctt ggataccatc atgttatcaa
    17101 tcatggcgct gggaataaca tcaaacgaga aggctttttg atcggtctcg ctcgaaggtg
    17161 ttgatgcgtt gtttatccat acgctgttgt atcgctggtt cagcccccta actacaacaa
    17221 atctatcatc aattattgaa gctcccggga cacgacggat aacctcggag gcatccttat
    17281 cctgtgaatt gtttatttgc tgcgccgata tgcctgttgc caccattagg ctcgccttta
    17341 tagagctaat catggctagg ttggtgctgg tttttcgggt ggctgttacc gtaacaccct
    17401 ccaactcagt agtgatttcc tcaagctcta tgctaacggt ggtggtttta ttggcttcaa
    17461 cccttatccc atcaagtatt gttggcttat aggatataaa tgatgcctgg acgctgtaaa
    17521 ttcccggctc caaattttcc agcttaaact ttccgtcaaa gtcggtaatg gtacccttaa
    17581 aagtaccctc gatgataacg ctggccccta ccagggtttc gtcttttccc atatcgacaa
    17641 ccgttcccac aatagatccg gtctgggccg atgctgatgt tgcaagtaag aaacatagaa
    17701 gagtgaacaa ataggctatt cgtctgttat tagttctcat agtttataaa cggtggttta
    17761 tattttttgc tgcaaagaaa ctggtaaact tcaacctgaa gattaacgca actttaatat
    17821 attgttaacc gccatcagga atgaggccgg attttttaat gttaaattaa ctttaccatt
    17881 ttattttata aatatctttg tatcaacata aaatgcttaa aacgggataa atgagtaaac
    17941 ctggttaccg aattcttatt gtagatgatg agcccgacat tattgagttt ttaagctaca
    18001 acctgaccaa ggagggctat gaggttgata ctgctacaaa tggaaggcgg gctatagaga
    18061 aagcaacaga attcagtccc gatcttattt tgcttgatgt catgatgccc gatatggatg
    18121 gaattgaaac ttgccaggag ttacgggcaa atccgttaac aaaagactgc ctcattgctt
    18181 ttttaactgc acgtagcgag gactatagcc agatagcagg ctttgacgca gggggcgacg
    18241 actacatttc aaagccaatt aagcccaaag tgctaattag ccgtattaag gccctgctta
    18301 aacgatctgg ctcgctggag gaagcacctg aaccagcacc cgaaattcct gaggagggaa
    18361 tctacatcaa cagggaacgg tatcttgttt acctcgacgg cagggagctg gttcttccaa
    18421 aaaaggagtt cgagctgctg gccctgcttc aaagtaaacc ccaaaaggtt tttacccgtg
    18481 atgaaattta ccaggcaatt tggggcgacg acattgttgt gggtgacaga actatcgacg
    18541 tgcatattcg caagctgcgc gagaagcttg gagaccaata cattagaacg gtaaagggtg
    18601 ttggatacaa gttcaccgtc taggctgcat ctccccaggt agctataagg gaaatttctt
    18661 ggaagcaacc taaattaatt ttacctatat ggagcttttt tcgtggaaaa acgactacag
    18721 cgttaaggtt ggaagcattg actccgatca tcagaagctg ttcgacatta ttaactcact
    18781 gtttactgcc atgtcgaagg gtgagggcag gcaggttatc tccggtttgg ttaaagagct
    18841 gcagagctac accatttacc atttcaaccg ggaggagaca tttttcaggg ttaccagcta
    18901 ccccaatgct gccaaccacc ttgagcagca taaaattttt gttagcaagg tgaaagagta
    18961 taaggaaaaa attgacaagg gaaacaataa cttttcgccc gaaatgttga cctacctaaa
    19021 ggattggttg cttaaccata tccttgttac cgacaaggcc tactctgatc acttcacaaa
    19081 gaacggcatt gtatagaatc cactctccaa caagggaggg ggtattcttt aagctacaat
    19141 ttttcttacc acagctaatt ttaggctgat aatgggaagg attatacact ttgaacaggg
    19201 tatcaatatt tcagattctt ctaatttcaa ccaggagtgg taattttata ttagtagcat
    19261 tacggttata atttgatatt ttctaccttt ctttcaacta ttttcaaaaa gaatatttgc
    19321 tatggagcta atttcgtggg aaagcaacta cagcgttaag gttggcagca tagatgccga
    19381 tcataagaaa cttgtggaaa tactcaactc gctttttatg gccatgtcga agggtgaggg
    19441 gagtaagatt atctccgact tagttaagga acttcagcgt tatacaattt accattttag
    19501 cagggaagag tcttttttta ggattaccgg ctacccgaat acccaaaaac atattgagga
    19561 gcataagcag tttattggta aggtgaaaga gtataaggaa aaaattgaca agggaaacaa
    19621 taacttctcg tccgaaatgt tggcctacct aaaggattgg ttgcttaacc atatccttgt
    19681 taccgacaag gcctactcgg agcattttgt aaaacacgga atagcctgaa ttgctgatat
    19741 gaagaagttt ggaggacgaa atgacttcca aataggcttg gatagtgttt gcctttaggg
    19801 caaatatcac ttctatcaat atctcgtcct acttgaagtt ggccaccatt tccattcttc
    19861 gttgggttcc cttctcaagt ggaacgaggg ttaagctggt gggtataacc gaatacttgc
    19921 tttccggtct aagctggcca agccacttaa atccttttag ggtcatatct tcgggttcca
    19981 cttgttcaat aggaatgagg tttgaagttg gcttctgctt gaataccacc cggctgacct
    20041 tattgttctt aatgtggacg tcaacattag aaccttcagc cctgttcacc atggtgattt
    20101 gacccttgtc cctaacgaag taaaccacct gtccgtttcc cgtaatatcc attcgggtaa
    20161 gctggccatc ggaaaacctg gctaaaatgg ttttacccct catctggcta aagtatgcgg
    20221 agtcctcacg ggatgatatg aaggcattgc ccaaaaagtc agcctggctt atctttttgt
    20281 tgcttatatg gatacggatg aaatcggcag tcatctggtt tgcctcgttc cacataaccg
    20341 ggttgccata catctccaaa acagagtcgc gggtgttgta gatcatggaa tcgcaaacac
    20401 cctgaatatc gtggcggtag tacatcaccc tgcctattgc ctttaccaat ctataggatg
    20461 agtcggtttt ctgattatca cctgttttgg ctgtaatcat gagcagcttg tcggtgcgaa
    20521 ggaataggct gtcggaatca tcaatcatca tcatgtaggg ttgctcggta atttcggctt
    20581 cctctttttc ctgccagtag ctggcctttt ggccgtaaat gtaggtgcgg tttaccgtgt
    20641 caaccatcac aacatttcct attccacggg catacccatt ttgcttatcg tagtaaatat
    20701 tttcgccaaa gagtttttga gagttgctta tgagataggc gttattttgg aagttcgatt
    20761 gttgtagttt tcggttatgc cagccttttt cgcagtatat aaagttttct ccgtcataga
    20821 taaaggttgg tccaaaaaag taggcaacct catttttggt gtggtacgaa agggagtcag
    20881 taaaggcatt accatcggga ctttttagga ctactttatt ggcaaaggat atcaggttgt
    20941 cgttgttgtt gtagtagcct tttatgctgg ttaaggtgga tgagtcgttg gaggttgcca
    21001 ctcctccggt ggtgtagaaa gccaggcttg ttttcgagtt gaaatccagg gcgtcggtaa
    21061 ccagtgtaaa atctttgtca accattttaa cctccttgcc ggttagcctt ccctcggagg
    21121 ttctcccgtc atagtgcagc ttgtcgccat aaatattcga acttccctgc gaaaccacca
    21181 cgccattaaa ggcatcgaac cagttttcat tttggctgta gtatgcgcta tcgcacctaa
    21241 cacgggtgtt atcgtagaaa aaacggacgt tgccaatgta tctgacaatt ttctgatctc
    21301 ccaggttcag gtttttcatg gctttggcgc cctccagctt aatggtggtt ttctgctggg
    21361 cagctgaaaa tgttgggtgc aggtacgtaa accctaaaat aaataggaaa attctggttg
    21421 tgctggttga agctttttgc attaccactt cttgctactt aatccatcct ttagagcgaa
    21481 agctgcagtt tctaaatggc tcatcaaccc tgatgtaggt tgattgaatt ttcttgtcca
    21541 accactcttg cagaatttcg ttttccatac taaccttagc tatattctga atgatggcgt
    21601 agtcttcctt aaggttggct ttgtggggtg gaattattcg cttcaccttg ataattttga
    21661 ataccacgtt ggcatgttcg tctcgtgatt cgaatgggct gctcatttcc ccctccttga
    21721 gctccctaat ggcgtagaag tcggcaggtt gaagctgctc cttttcaaaa agagaagtat
    21781 tgttgtagga gttgatgacc agccctccgt ttagaaacga ttttttatcc tgagaaaatc
    21841 tcatggctgc agcttcaaag gtcaagctat cggctttaat gagggtggca atgctatcga
    21901 gctttgacat ggcttgtgcc aacatatcac ctgagaattg gggtttcatc agaatatgcc
    21961 taacatttac ctgattgtcg cgtttttcaa ttagctggat gatgtggaag ccatactcgg
    22021 tttcaactat ttggcttatt tgaccctcct ttaggctaaa cgctgcatcg gcaaaggcct
    22081 tc
//
LOCUS       DAOPHX010000030        20948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_2700_length_20948_cov_6.292490, whole genome shotgun sequence.
ACCESSION   DAOPHX010000030 DAOPHX010000000
VERSION     DAOPHX010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 20948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20948
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2700_length_20948_cov_6.292490"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PK502_03715"
     CDS             complement(<1..515)
                     /locus_tag="PK502_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99956.1"
                     /translation="MAKRLTRREFIRDSLLAAGGLYLTRFTSLRATAAGNQKPWKWSR
                     EAMFYTLTPRGARCEICPNECTIRPGESGDCRNRENTNGKLYSIAYGNPCSVHVDPIE
                     KKPLYHFLPGSQSFSIATAGCNFACMNCQNWEISQTSPRKTRNIELFPEQVVEQCLLS
                     GSKTIAYTYSEP"
     gene            complement(1107..2873)
                     /locus_tag="PK502_03720"
     CDS             complement(1107..2873)
                     /locus_tag="PK502_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPG99957.1"
                     /translation="MRIFGANISLQVCTFLKEFLKETSSFENANIMLFDAFGINTFFT
                     SNDDFQGLKESVSLVAIGSVEEPDRAEYGDFQTNKNLSNKVVNYLVKRNISPEIIIEP
                     TCGNGNFIVASLSNFKAAKKVFGIEIYKPYVWETKFSILDFYLSNPDNHKPEITITHC
                     SVFDFDFKNISKQFPAEKILIIGNPPWVTNSKLGSLNSNNLPKKSNFKNQNGLDAITG
                     KGNFDIAEYITLMLLDTFQTHNGYLALLVKNSVIKNIVFDQKDKKYRIGEIEKYCIDS
                     KMEFNVSVEAALLYCQLNLTPSFECNEFDFYSFEKRLSFGWLNSKFVSNLADYNETKG
                     IDGLCPFEWRQGIKHDCAAIMELERINDHFVNNQSDEIKLDEDLVYGLLKSSDLKNSV
                     IMNSRKYTIVTQTKVGQETSYIQHLYPDTYSYLSQNISIFQARKSSIYNGNPLFSIFG
                     IGDYSFKPFKVAISGLYKTYHFSLVLPQNNKPMMLDDTCYFIGFDKIEYAVYSFILLN
                     SKTTEAFLKSITFPDAKRIFTKDILMRIDILSLSKVIPQNEIEKQIVFLNGKYNFDIN
                     LSQWDSFISEMTPKKEKQLKIF"
     gene            complement(2873..3547)
                     /locus_tag="PK502_03725"
     CDS             complement(2873..3547)
                     /locus_tag="PK502_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HPG99958.1"
                     /translation="MKEKLTIEKLKAEAKVFCLTESKIQNKSLFGVTDGKAVGTYIEH
                     KFQEQLNAKYKITIGSSANGIDLPSDDILTDIKVTSIKQPQSSCPFIDAKQKIFGLGY
                     NLLVFVYDKIDDFQSKTTRLNFVSCSFILKERTSDYTTTYRLREMVKDKANVEDIVAY
                     LNDKNIPADEITLNQLAKQILKTPPVQGYLTISNALQWRLQYQRVVTLEKAVQGISRI
                     IDKVVR"
     gene            4637..4969
                     /locus_tag="PK502_03730"
     CDS             4637..4969
                     /locus_tag="PK502_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99959.1"
                     /translation="MKKLLILLCFLGFVNLTLSAQNIYIRGHLQVIYIGPEGMDVICN
                     PPYDRDCVSIISEPGLPAKATAEVSDKGVTKIQPRVQVVGETLNSNGLPTKIYRIRKD
                     AVKEVVKN"
     gene            5019..6536
                     /locus_tag="PK502_03735"
     CDS             5019..6536
                     /locus_tag="PK502_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99960.1"
                     /translation="MRNILTTFVFLCLSLPAISRVGGVEIFFAIKPSDCISCMAPIRD
                     ILAEIEKQELMEQVTFIVRDVPEKALPKYTKEVLKIPNSINVVSSEPLFSKYSFYNAS
                     AITVIDNSTVPIQRITFPLAYYFVNKTMFVERLSKSNKTIVALERVKLKESLGGIRML
                     SISNDSLLFINDRLFNTISLFSAGSGKLVGNFTLDSAWYAAPYSKDTFKLRQIWMNQH
                     VLERASYPQYYFKGVFNHRGRTIISAICYYPFTTGKDFFILPKNLFFMLDTLLMPISY
                     RWLYIDKETPIVNFYNAVFFQDDNLITTTFDVVNNRLNFNPDFLYYFKPEGDSLIFDK
                     SLHVEPPSFIKNGYSMNGFSLNYISEYMGNITVWFSYYPEFFDLRLEKWVSIDTGYVD
                     SKISTNTFEQGQPVNFQVLGVWAEANQYSMILRFGLQVYYMVQADKNIIQSALIYTLS
                     EGNQLQMVRKSKNEFVGTDYNSKTGDTEIIKFTINGVQTFISPSLNEACAFVVPM"
     gene            complement(6893..>7774)
                     /locus_tag="PK502_03740"
     CDS             complement(6893..>7774)
                     /locus_tag="PK502_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99961.1"
                     /translation="VYNTLTAVFANVEVVPGERDYLLASDGEILIDISRLAVERGLAE
                     LNTYVNPDFIDDDYLASRNRFFHASILSDARLNTDNHPYPVFLFTMSYLGQFGSNHLV
                     WMLVGLAVVLLPVFFLGKPLRGMFLAGFSGASAEMIIILMFQVLFGFLYAGIGLIVAL
                     FMAGLAVGAYVLPRFIRLSVGSLTIAMAGYFALIPLIWMLRDVAAVWLLLLVISLFTL
                     IPSVLVGYQYVLWTSAVADRANPAAMSYSADLWGSTLGVVVVTLALIPLLGVVQTAAV
                     LAALNLAGRLLIQPRNR"
     assembly_gap    7775..7874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7875..9076)
                     /locus_tag="PK502_03745"
     CDS             complement(<7875..9076)
                     /locus_tag="PK502_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99962.1"
                     /translation="MNMPYHLLLIGMGIGVAYIMGSLLVYLGILSKNLHRKIWNYTLL
                     AVFLGCVALGLLLAIQISYKLDWPFVSVALKWHVNLGIAMSLVAAIHIFWHVRYFTGT
                     NRKCHGKIKQKEPPIWVTSYELGILAFAAGLASISFQILILRQVTKVFQGNEFTQTWI
                     IGIWMLLTGVGSALGRRAKSGSESFPVISRLIIFQCVAAAVVTFSLGYIKNLIFPVGV
                     LSPPLATIALSSFLLIPVAIPAGIVYALMVSRFKADDSCTSRIYSMESLGSMAGGVLV
                     SLIIIHFLTVYQSVLVIGVIATLIVSVIRPGKGIITVPLVFLLVMAAFMAAKADRLIE
                     QNIMHGQRVLAVKESPYGSITVTDYGGQLNFFENGLLLFGTGNPVAAEEGVHYAMLQR
                     SNPSEILLV"
     gene            complement(9088..10554)
                     /gene="amrB"
                     /locus_tag="PK502_03750"
     CDS             complement(9088..10554)
                     /gene="amrB"
                     /locus_tag="PK502_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPG99963.1"
                     /translation="MESSCKGQTGGSSRGVDRQPAVAGSFYPASREQLVKDLQGYFDA
                     AEVSLTSQPIALIVPHAGYVFSAPVAAAGFKQLDRERKFKHIFLIGSSHTMYFNGVSA
                     YTQGHFITPLGRVKVDDLAKELADRYDFINDDPMPHEREHGLEVQLPFLQYWLKHEFC
                     IVPLIIGGESQSTSTLLAEVLAPYLNEENLFVISSDFSHYPTYAEATKSDKSMADAIV
                     SNSSSAFMKAKLNSENSGADNLVTAMCGWTSGLTLLKMTEKIKGITYQPLIYMNSGDS
                     PYGEKDRVVGYWAIAAVRESNRSGDFSLTEEDKMELLKLARKTITDHLNGLKPTPPDL
                     QNLSANLKTNAGAFVTLRRDGQLRGCIGSFSTTTPLYQVVMAMAVAAATEDYRFPPVT
                     RNELGEVEIEISVLTPMKKISSIDEIELGRHGIYIRKGGHSGTFLPQVAAETGWSLEE
                     FLGHCARDKALIGWDGWRDAEIFTYEAIVFEEKDFKMN"
     gene            10928..11338
                     /locus_tag="PK502_03755"
     CDS             10928..11338
                     /locus_tag="PK502_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99964.1"
                     /translation="MPAKKKEILTVEQGRPFMFIALSTTLNLNKLLWAASNHCGLRLV
                     RNAELEQLIGVSSFTDRTSKPGLIVSLFPNRSSNGLVVGQLPNVDYIVEINGNAAEAE
                     VKILSACLRGFQGIQAAITINPSKIKISEPFCPE"
     gene            11462..13045
                     /locus_tag="PK502_03760"
     CDS             11462..13045
                     /locus_tag="PK502_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPG99965.1"
                     /translation="MSRKLLWILTVVIAIASIGLIFLQSRWIGVALQIKEEQFVQTAS
                     LAMERIIDEVEKQETVFQVIDEIKPYYSVNTKSSARLDYQQNILNKTKSGIRTKQINQ
                     QVLTFSSLDSVKIPSITKKLWDSSLVSINNGDLHIPSLSKGKNLKNIDLGLDEKLVNK
                     TIFVENIVDKMIRVELPIQERISQVQLDSIVRRELLRKGIKARYEYRITNERDSTIYA
                     SPKFKADFKGLVLKDKLFPNDFFARRYFLTLYFPNQKTYLLNSLGRITLSSLALTFLI
                     VFTFSVTLYIIFKQKKISEIRNDFVNNLTHELKTPISTISLAAQMLNDKSIPLERKNL
                     DYLGTLVADESKRLGLQVEKVLQMAIFEKAKLKLRLKEVNVHEIIKRVSNNFALQLQS
                     ENGVLKLELESDKPVVLADEVHITNVVNNLLDNAVKYKKGNPEITIFTKNSTDGIVVT
                     FQDNGIGISRENLRKIFDQFYRVPSGNIHNVKGFGLGLSYVKKIVEAHGGRIWVESTP
                     GEGSLFSIFLPWNGPSEKK"
     gene            13073..13765
                     /locus_tag="PK502_03765"
     CDS             13073..13765
                     /locus_tag="PK502_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPG99966.1"
                     /translation="MEKRTIRVLLAEDDENLGFLLKEYLQAKEYDVDLYKDGEKAYRG
                     FQNNYYDICILDVMMPIKDGFTLAKEIRMANPAMPILFLTAKSLKEDVIEGFSIGADD
                     YMTKPFSIEELLMRIEAILRRTRKDSPDSEQSSFQIGRYLFDATKQTLQLDDEVRKLT
                     TKESELLKYLCINKNSLLDRNFALKTIWVDDSYFNARSMDVYITKLRKYLADDPTIEI
                     INVRGKGFKLIF"
     gene            13889..14269
                     /locus_tag="PK502_03770"
     CDS             13889..14269
                     /locus_tag="PK502_03770"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPG99967.1"
                     /translation="MDKIIIEPTLETPRVVLDRDSSIIEFSGNSLPEDVNTFYAPILE
                     WIDNYVDNPNQQTEVSMNFDYYNTSSSKMILRILERFRDIHRKGYPVVVNWLYMEDDE
                     DMVEAGEDFADHLKIPFKFIPIQH"
     gene            14293..15765
                     /locus_tag="PK502_03775"
     CDS             14293..15765
                     /locus_tag="PK502_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HPG99968.1"
                     /translation="MATPSKLKVFSNRLFGRFKSTEEVESNRKALEVEYYEWVEFAQS
                     DDYKRFEELKSWEATKEYLTVKKEVEAIKYSESPEQREEQELKKLSRSHSIRNFLKIA
                     KSETPDFYQKMEHSQLVKELKELQQFIATPDYQAKKNQYRKDNSAEYQKELRLKELLS
                     NPDIKKYLKLEKENDLKEYFQTENSEQLKRYNELSEKVSTEDFKNRKAYLLSKNKFEQ
                     TDAYKNLQEFKQLESSEKIKWFFRLNRSNKFDDLKAWNLAFYDDFDSKNIDPHKWLTK
                     FFWGEALVGKPYSFTTDNQNYTEKGNIEIGNSTLKIITRKEASEGLAWDKKFGFTPKA
                     FAYTSGIINTGHTFRLKEGRIEAKVRMSSVQGISHAFYLVGNEMLPEVDVFLKNNAKP
                     SGISAAYFWPNGKSKVAKSVSSIGGLKLGENFYIFGVEWDSNNIVWTINGIPYKVEPN
                     RHPGISYYMVFTSAVKGSVDDSLLPATLELDWVKCWEKKE"
     gene            complement(15789..16544)
                     /locus_tag="PK502_03780"
     CDS             complement(15789..16544)
                     /locus_tag="PK502_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99969.1"
                     /translation="MNSHSALRRLGYTSDQAAIMRRYISEQQGWIAHLENSRQIIIES
                     LLRQKAKTVRILGSGNLLDVPVDEILQHGSTLLLTDIIHPPQVVNRFYGASSVSFETV
                     DLTGGLVDFIAQRHARKLTFFELENFIRNITLPTFDEDLVVSLNLLSQLNDIPIAYLR
                     HKGKLSNEQLHELSEQIQQRHVDQLPVGRTLLVSDYEEEYYDEDGNLVGANPTVFVRI
                     PGCTIREWSWQFDTQMTYHEDFKTVLRVTARQW"
     gene            complement(16557..18344)
                     /gene="lepA"
                     /locus_tag="PK502_03785"
     CDS             complement(16557..18344)
                     /gene="lepA"
                     /locus_tag="PK502_03785"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005806468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPG99970.1"
                     /translation="MEKIRNFCIIAHIDHGKSTLADRLLELTHTLSEREQQDQVLDSM
                     DLEREKGITIKSHAIQMVYPYGNETYTLNLIDTPGHVDFSYEVSRAIASCEGALLVVD
                     ATQGIQAQTISNLYLAINHDLEIIPVINKIDMDSAMVEDVKDQIVDLIGCKRDEILAV
                     SAKTGFGVDAVLEAIVNRIPPPKGDPEAPLQALVFDSMFNSFRGIIAYFKVVNGTIRT
                     GDRVKFVNTGFEYTADEVGILQLKLQPRNQISAGDVGYIISGIKESSEVKVGDTITHV
                     DSPCDKAIAGFEDVKPMVFAGLYPVDADEYEDLRSSLEKLKLNDASLTFEPESSLALG
                     FGFRCGFLGLLHMEIVQERLYREFDMDVITTVPNVSFRVHTTKGEIVEVHNPSGLPGP
                     TVIDYIEEPIINAQVITKSEYLGTVIKLCIDRRGVLKNQVFIASDRVELTFRLPLSEI
                     VFDFYDKLKSISRGYASFDYFLDGYQEAKLVKLDILLNGEMVDALSSLIHIDHAYDFG
                     RKMCEKLKDLIPRQQFDIAIQAAIGAKIIARETVKAVRKDVTAKCYGGDITRKRKLLE
                     KQKKGKKRMRQIGSVEVPQEAFLAVLKMD"
     gene            complement(18480..19277)
                     /gene="kdsB"
                     /locus_tag="PK502_03790"
     CDS             complement(18480..19277)
                     /gene="kdsB"
                     /locus_tag="PK502_03790"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPG99971.1"
                     /translation="MKTLGLIPARYASTRFPGKPLAIIAGKPMVQWVYERASGVFDHC
                     YVATDDQRIVNVVQGFGGQSVITSEHHRSGTDRCAEAMEILQNSLNISFDVVVNVQGD
                     EPFIQPEQLQRITECFSDRTVQIATLVKAFGSNEDIFNSNAPKVVVSTEGNAIYFSRS
                     AIPFVRGKAQAEWQLAHRFFKHIGLYAYRPDILRQITRLPQSSLELAESLEQLRWIEN
                     GFTIRVLETDQETLAVDTPDDLNRVKEFAMKLLNQPDKRDTKQQPSA"
     gene            19797..20705
                     /locus_tag="PK502_03795"
     CDS             19797..20705
                     /locus_tag="PK502_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPG99972.1"
                     /translation="MSHQNESVKSILLALLANLGIAITKTIAAVVTKSGAMMAESIHS
                     FADCANQGLLFWGLKASRKKPNPEHPLGYGKEIYFWSFIVALILFSMGGLFSVYEGIH
                     KVSAHEGLRNTNVAIIVLVVSMVLESISLYGCLKQIRKVRHGESLWKWYKTSRQSELV
                     VVLSEDIAALLGLTLALVAVILSIATGNPIYDAAGSIGIGILLVIVSCFLAVKVKGLL
                     IGQSVDDETSNKIRKLIESRPEVEKIFNLITIQLGPQVMVAVKAKMKATDNINQLISN
                     INACEEALTQEISTIQWVFFEPDLKD"
BASE COUNT         6176 a         4619 c         4430 g         5623 t
ORIGIN      
        1 ggctcggagt aagtgtaggc aatggtcttc gatccggata gcaggcattg ctccaccacc
       61 tgctcgggga agagttcaat attccgggtt tttcgagggc tggtttgtga aatttcccag
      121 ttctggcagt tcatacaggc aaagttgcat ccggcagtag cgattgaaaa ggactgcgaa
      181 ccgggcaaaa agtggtaaag aggctttttt tcgattgggt ccacatgaac cgagcagggg
      241 ttgccatagg caatggagta aagcttgccg ttggtgttct ccctgttacg acagtcgccc
      301 gattccccgg gacgaatagt gcactcgtta gggcatattt cacacctcgc accccgtggg
      361 gttagcgtgt aaaacatagc ctccctgctc catttccatg gcttttggtt gccggcagcc
      421 gttgccctta gacttgtaaa acgggtaaga tataaacctc ccgctgccag caggctgtcc
      481 ctgataaact cacgtcgtgt taacctttta gccacgctac ctagctatac gtacacaaaa
      541 tgttacccaa aacttacctc ataacggtat cattagccct acctgaggtg ggcaataaag
      601 ttaaccatta ccatacattt aagcaaattt gggggggtaa cccctgccag acaacggcag
      661 caaagaacca tatacagaga agctggtcgg taccgataat aggcgtagct tccaaaattc
      721 gtcaggtaaa ctcccttgct gccgctggac ttccgtcgtg tgggagcaag agggtctgtt
      781 atgcgctttc tcataatggc tgaggccata tacagtaaat tattgggtgg ttacctctct
      841 tcccaaatgc acctaattta gatttgacag aacgtacaaa ttatcaatga aacccttatg
      901 gtatatgggc ttagttatgt ctttgttcat tgttttcatc cgtataaaat ctaatattat
      961 taattgacaa taacttacga tttcgcctta agcactcgtt aacgttcgga tggaacacca
     1021 atattttatt ggcttcgccg agctctccgc cagctggcgg atcggttcat ccctgtttgt
     1081 gcaagctgcc ttacatgagt gtttcatcag aatattttta actgtttttc tttcttaggc
     1141 gtcatttcgc taataaaact atcccattgc gacaggttaa tatcaaaatt atacttgccg
     1201 tttaggaaaa ctatttgctt ttcaatttca ttttgtggaa ttacctttga aagggataat
     1261 atatctatac gcattaaaat atctttggta aaaattcttt tagcatctgg aaatgtaatt
     1321 gattttaaaa acgcttctgt tgttttagaa tttaaaagta taaacgaata aactgcatat
     1381 tctattttgt cgaaaccaat aaaataacaa gtatcatcta gcatcattgg tttgttattt
     1441 tgtggtaaca ctaatgaaaa atgatatgtt ttatacaaac ccgaaattgc aactttgaat
     1501 ggtttgaaag aatagtcacc tataccaaat attgaaaata atggattgcc attataaata
     1561 ctagattttc ttgcttgaaa aatggaaata ttctgcgata aataagagta tgtatcagga
     1621 tataaatgct gaatatatga ggtttcttgc ccaacttttg tttgtgtaac aatagtatat
     1681 tttcttgaat tcattattac agaatttttt aaatcagaac tttttaaaag accataaacc
     1741 aaatcttcat cgagttttat ctcatcagat tggttattca caaaatggtc gtttattctt
     1801 tctaattcca taatggcggc gcaatcgtgc tttatcccct gcctccactc aaaagggcat
     1861 aagccatcaa tccctttcgt ttcattataa tcggcaagat tagatacgaa cttagaatta
     1921 agccatccaa agcttaatct tttttcaaaa ctatagaaat caaattcatt acactcgaaa
     1981 cttggggtta aattaagctg gcaataaagc aaagccgctt caactgaaac attaaattcc
     2041 attttactat caatgcaata tttttctatt tcacctattc tatacttttt atctttttgg
     2101 tcgaaaacaa tattttttat cacggagttt tttaccaata gcgccaaata accgttgtgc
     2161 gtttgaaagg tatctaaaag cattaaagta atatactcgg caatatcaaa attgcctttg
     2221 cctgtgattg catctaaccc gttttgattt ttgaaatttg atttttttgg aagattattc
     2281 gaattaaggc tacctaattt tgagtttgtt acccaaggcg ggttcccaat tattaatatt
     2341 ttttcggctg gaaactgttt tgaaatattt ttgaaatcaa aatcgaaaac gctacaatgt
     2401 gtgattgtaa tttcaggttt gtgattatca ggatttgaga gataaaaatc gagaatactg
     2461 aattttgttt cccaaacgta aggtttataa atttcgattc caaatacttt tttagctgct
     2521 ttaaaattcg ataacgaggc aactatgaaa tttccatttc cacaagttgg ctcaataata
     2581 atttcggggg atatatttct ttttactaaa tagttcacaa ccttatttga tagattttta
     2641 tttgtttgaa aatctccata ctctgcacgg tctggctctt caactgaacc tatagcgaca
     2701 agagaaacag attctttgag tccttgaaaa tcatcattac ttgtaaaaaa tgtattgatg
     2761 ccgaaagcat caaacaacat tatattggca ttttcaaaag atgatgtttc ttttaaaaat
     2821 tcttttaaaa acgtacaaac ttgcaatgaa atatttgcac caaatattct cattacctga
     2881 ccactttatc aattattcta cttatacctt gcaccgcttt ttcaagcgta acaactcttt
     2941 gatattgaag tcgccattgt agagcgtttg aaatagttaa atacccttga accggcggtg
     3001 tttttagaat ttgtttagct agctgattta gagttatttc atcggcagga atatttttat
     3061 cgtttaggta ggcaacaata tcttctacat tagccttatc cttaaccatt tcacgcaaac
     3121 gataagttgt tgtataatcg gaagttcgtt cttttaaaat aaatgagcaa ctaacaaagt
     3181 taagtctagt agttttggat tggaagtcat caattttgtc gtaaacaaat acaagtaggt
     3241 tgtatccaag tccaaaaatc ttttgtttag catctataaa tggacaagat gattgaggtt
     3301 gttttattga tgtaacctta atgtccgtta atatgtcgtc agatggcaaa tcaattccat
     3361 tggctgaaga acctatggtt attttatatt ttgcatttaa ttgctcttga aacttatgtt
     3421 caatataagt cccaactgct tttccgtcag ttacgccaaa aagtgattta ttttgaattt
     3481 tcgattcagt taagcaaaaa actttggctt ctgctttaag tttttctatt gtcagttttt
     3541 ctttcatctg atttccttac attaagttac aaatttaatc aatttttcag tccgatttgt
     3601 acgctgtcag ttttctttgc atgaggcata acaggcaggc agctggtttt gtcatattag
     3661 gtttatctgt tgttcagtgc tagtaggcag gctccttgct cggcgaagtc tgtaacttcg
     3721 tcgttttcat gcagcggtct ctgaccgcat ttgatatcat cagtaaactt tccaatgggg
     3781 taaatataca cgatttcaag cggtttaact ttcgttcggt taaagctgtg tacatcagcc
     3841 gaatctattt ccctcgctgc cgctggactt ccgtcgtttg gcaatctcat ccagacctcc
     3901 cgaccagcgc agtcaacaaa cccatcatta aatttacaac tcataatccc gccgagttgg
     3961 cttttttaat agctactaaa gctcactctc cttttctcta ctgccacacg gaaggactcg
     4021 tgcggtagca gaaaaatcaa gaatttgcac aagggttgag tacttgtttg gatttgtagg
     4081 aaacagcatg aacggttcgt ttatacgcac tattggaatt tcaagggcta aagcaaaaat
     4141 agggatgatg aacctgacgt acaatatttt ccgctgtact caattaaaaa tagtggttgt
     4201 aatgggatag gtatgaccgg acataaaaaa taatttagta tgtaactgat agttaattaa
     4261 ataaaaaata agaagagtaa tatttgattt tttcaaaatc aaagaataaa tttgtttcga
     4321 gcattagcac aaaaaaagta gtttttcgag gtgcccttta gtaatgggag ttttgaacgc
     4381 tctggttgag aggtatagat aatcttgcca tacgactaaa gtcgtgcggc aggaaggggg
     4441 aaaaatgccc tcaatttctt agtgtctata ttgtggccgt tagggtcaat ttgacataga
     4501 tgctcggaaa aattgaacca ctgtttggct tagaaaaaca taccgatgtg actacacgaa
     4561 aatttaaata aagggtgaag aagttgaccc gaacacacct gaaaccaaag tagggcaaaa
     4621 ttaaaaacaa atcaaaatga aaaagttatt aattttatta tgttttttgg gttttgttaa
     4681 cctgactctt tctgctcaaa acatctacat tcgaggccac cttcaggtta tctatattgg
     4741 gcctgaggga atggatgtta tttgcaaccc tccgtatgat agagattgcg tatcgattat
     4801 atctgagcct ggactaccag ctaaagctac agctgaggtc agcgataaag gagttacaaa
     4861 gattcaaccc agagttcaag ttgtgggaga aacactaaat tccaacggtt taccaacaaa
     4921 aatatatcgc atacggaaag atgccgtaaa agaggttgtt aaaaactaga ttgctaactt
     4981 aaggggtggt atccccatcc cttattttat accaaaccat gcgaaacata ctaacaacat
     5041 ttgtcttttt atgcttatcg ttaccggcaa tcagcagggt tggaggggtt gaaattttct
     5101 ttgccattaa accctcggac tgcatatcgt gcatggctcc catcagggat atacttgccg
     5161 aaattgagaa acaggagctc atggaacagg ttacctttat cgtaagggat gttcctgaaa
     5221 aggcgcttcc caagtacact aaggaggttt taaagatacc caacagtata aatgtggttt
     5281 catcggaacc acttttcagc aagtactcct tttacaacgc ttctgcaatt acggtgattg
     5341 ataactccac tgtgcctata cagcgaatca cgttccccct tgcttactat tttgttaata
     5401 aaaccatgtt cgttgagcga ttatccaaaa gtaacaaaac gattgtagca ctggaaagag
     5461 taaaacttaa agagtcgctg ggtggaatca gaatgttgtc catatcaaac gattcgttgc
     5521 tttttataaa tgacaggctg tttaacacca tttcgctgtt ttcagctggc agcggaaaac
     5581 tagtcggaaa ttttacgcta gacagcgctt ggtacgcggc accctatagt aaggatacct
     5641 ttaagctaag acagatatgg atgaatcagc atgtacttga aagggcatct tacccccagt
     5701 actattttaa aggagttttc aaccatagag gtagaactat aatttctgca atatgctact
     5761 acccgttcac aactggtaaa gactttttta tactacccaa aaaccttttt tttatgctgg
     5821 acacactatt gatgcccata agctacaggt ggctttacat tgataaggaa accccgatag
     5881 ttaattttta taatgctgta ttttttcaag atgataacct catcaccacc acctttgatg
     5941 ttgtcaacaa ccggctaaac tttaaccccg attttttata ctattttaaa cccgaaggcg
     6001 actcgttgat ttttgataaa tccctacatg tagagccacc aagttttatc aagaatggat
     6061 attctatgaa tggtttttca ttgaactaca tatctgagta tatgggcaat attaccgttt
     6121 ggttctccta ctaccctgag ttcttcgacc taaggttgga gaagtgggta tcaatagata
     6181 caggctacgt tgattctaaa atatcaacta acacctttga acagggtcag cctgttaatt
     6241 ttcaagtgct gggcgtatgg gctgaagcaa accagtacag catgattcta aggtttggcc
     6301 tgcaagttta ctacatggtt caagctgata agaacataat tcaaagcgct ctaatctata
     6361 cccttagcga agggaatcaa ctccaaatgg ttcgaaagag taagaatgag ttcgttggaa
     6421 ctgattacaa ctcaaaaacg ggagatactg agattatcaa attcacaatt aatggggtgc
     6481 aaacatttat ttcaccctcg ctcaacgaag cctgtgcctt cgtcgtacct atgtagcgtt
     6541 ctctgacctc ataagatact atcagtaagc tttccattgg ggtaaatata aacaatttca
     6601 agcggtttaa ctttcgttcg gttaaatttg tggacatcag cccactctgt taattcgggc
     6661 tttgccaggt aaatatagct aattgtaagt tttccagcgt atgtcatttc aaaaatagca
     6721 ggcgcagcca gctactgata gccctgcggc cgcagaacca ccgccgaaag cgtttagatt
     6781 atttcaacct gtaacatcag gcgggctgag gctaggttag tgtttccctt cactaatcca
     6841 gcccaacctt cagggggtta acttacgttg ccttaccgtt taaccgttgg aattaccggt
     6901 tacggggctg aattagtaga cggccagcca ggtttagggc ggccaggact gctgcggttt
     6961 gaacaacacc cagtagcgga attagcgcta gtgtaaccac aaccacacca agggttgacc
     7021 cccacaggtc ggcagagtaa ctcattgccg cggggtttgc cctatcggca actgcactcg
     7081 tccatagcac atactggtag ccaacaagga ctgatggaat aagggtgaag agggaaataa
     7141 ccagcagcaa cagccacact gcagcgacat cacgtagcat ccatatcagc ggaattagcg
     7201 caaagtagcc cgccatggca atggtcaggc ttccaaccga aagccttatg aatctgggaa
     7261 gaacgtaggc gccaacagcc aatccggcca taaagagcgc cacgataagc ccgatacccg
     7321 catacaaaaa accaaagagc acctgaaaca tcaggatgat tatcatttcg gccgaggccc
     7381 ccgaaaaccc ggcaaggaac attcccctta gcggtttccc aagaaaaaaa acaggtagca
     7441 aaactacagc taagcctacc agcatccaca ccaaatggtt gctgccaaac tgccccaggt
     7501 agctcatggt gaaaaggaaa accgggtagg gatggttgtc ggtgtttagc ctggcgtcgc
     7561 tcaggatgct ggcatggaaa aaccggttac ggctagccag gtaatcatca tcaataaaat
     7621 cgggatttac ataggtgttg agctcggcca atcccctctc aactgcaagc ctcgaaatgt
     7681 cgatgagaat ctccccgtcg gaggccaaca ggtagtccct ttcgcccggc accacctcaa
     7741 cgttggcaaa tacggctgtc agggtgttgt aaacnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnaccaac agtatttccg aagggttact acgctgaagc attgcgtagt
     7921 gcacaccttc ctcggcagcc acggggttac cggttccaaa aagcagcaaa ccattctcaa
     7981 aaaagtttag ctgaccgcca taatccgtaa cggtgatgct tccgtagggc gactccttca
     8041 ccgccagaac ccgctgcccg tgcataatgt tctgttcaat gagcctgtcg gcttttgcag
     8101 ccatgaacgc agccataaca aggagaaaca ccaggggaac ggttatgatt cctttccctg
     8161 gcctgattac cgatacgata agggtggcta tgacccctat aaccaggacc gactggtaaa
     8221 ccgtgaggaa atgtatgatg atgagcgata ccagcactcc gccagccatg cttccaagcg
     8281 attccattga gtaaatccgg gaagtgcatg aatcgtcggc tttaaaacgg ctcaccatta
     8341 aagcataaac aatacccgcc ggaatagcca ccgggatgag cagaaaactt gaaagggcaa
     8401 tggtggcaag gggtggtgaa agcaccccaa caggaaaaat caaattttta atgtagccaa
     8461 ggctgaaggt aactacagct gcagcaacgc actggaatat gattagcctg ctgataacgg
     8521 gaaaagactc gctccccgat ttagccctgc ggcctagggc agaacccaca cccgtaagca
     8581 gcatccaaat gccgattatc catgtttgag tgaactcgtt accctgaaaa accttggtaa
     8641 cctgtcgcag aattagtatc tgaaatgaaa tggaggcaag ccctgcggca aatgccagaa
     8701 tgcccaactc gtaggatgta acccaaattg gaggttcttt ttgtttgatt tttccatggc
     8761 atttcctgtt tgttcccgta aagtacctca catgccagaa tatatggatt gcagccacca
     8821 ggctcatggc aataccaagg ttaacatgcc atttcagggc aacggaaaca aagggccaat
     8881 caagcttgta gctaatttgg atggccagca gcaggcccag cgctacacaa ccaaggaata
     8941 cagccagaag ggtgtagttc caaattttac ggtgaaggtt tttggagagt atgcccaggt
     9001 aaaccagcaa gctgcccata atgtaagcta cgccaatacc catgccaatc agcagtaaat
     9061 ggtagggcat attcataacc ggcagattta attcatttta aaatcctttt cctcaaacac
     9121 aatggcctcg taggtaaaaa tctcggcatc cctccatccg tcccagccaa taagagcctt
     9181 gtcgcgtgca caatggccaa ggaattcctc aaggctccag cctgtttcgg cggcaacttg
     9241 tgggagaaat gttccggagt gtccgccttt ccggatgtat atgccatgcc tgcccagctc
     9301 tatctcgtca atgctgctaa tttttttcat tggggtaagc accgaaattt caatttcaac
     9361 ttcaccaagc tcattacggg ttacaggggg aaaacggtaa tcctccgtag cggcagcaac
     9421 ggccatggcc ataaccacct ggtagagtgg agtagtagtg gagaagctgc caatacaacc
     9481 cctaagctgt ccgtccctgc gaagagtaac aaatgcaccg gcattggtct tcaggttagc
     9541 gctcaggttc tgcaaatcgg gaggtgttgg ctttaagccg ttgaggtggt cggtaatggt
     9601 tttacgcgcc agcttaagca gctccatttt gtcctcttcg gtcaaagaaa aatcaccaga
     9661 cctattgctt tccctcacgg cagcaatggc ccagtaaccc actaccctat ctttttcgcc
     9721 gtatggcgaa tcacccgaat tcatgtagat gaggggttgg taggtaatcc ctttaatttt
     9781 ttcggtcatc ttcagcaggg ttagccccga agtccaacca cacatggcgg taaccagatt
     9841 gtcagcacca gagttttcag agttcagctt agctttcatg aatgctgaag acgagttcga
     9901 aacaattgca tcggccatgc ttttgtcgga ttttgtggcc tcagcatatg tcgggtaatg
     9961 cgaaaagtct gaacttatta caaacaggtt ttcctcgttt aggtatgggg caagaacttc
    10021 ggcgagcagg gtgctggtac tttgcgattc accaccgatg ataagaggaa caatgcagaa
    10081 ttcatgctta agccagtact ggagaaaggg gagctgcacc tccagcccat gctcccgctc
    10141 gtggggcatg ggatcgtcgt tgataaaatc gtacctatcg gccagctcct ttgccaggtc
    10201 gtcaacctta acacgcccaa ggggggtgat aaaatgacct tgggtatatg ctgatacacc
    10261 gttgaagtac atggtgtggc tggagccaat caaaaaaata tgcttaaact tcctttccct
    10321 atcgagctgc ttaaaacctg cagctgcaac aggcgcggag aaaacgtacc cggcatgggg
    10381 aactattaaa gctattggtt gagatgtaag gctaacctca gccgcatcaa aataaccctg
    10441 caaatccttt accagctgct ccctggaggc tgggtagaac gatccggcaa ctgcaggttg
    10501 acggtcaact cccctactac ttccacccgt ttgcccttta catgacgatt ccattgctac
    10561 tagtgttatt agtaaactta tcgttacaaa atatcgtgat tgaaagcccg gcatgatagc
    10621 acgaatattt acctttccca ttcaaagtta tttcaaaata aaagaaaaat caagttcata
    10681 tcactccagc cagctaccat aagacaactt tgggaaaagt tgctaaaacc tattcaaatt
    10741 cccgatggtt agaaggctaa cggttggtcg ggaatagggt aagcgaggag ttgaaattca
    10801 gaaatgctaa aaattaaccc gttcgcattc agcttccaac accgtccgat ttcgttaaga
    10861 taaatttagg ggttcggtaa gattgtttaa aaacagggtt atatttgtgc tagactagta
    10921 agctaaaatg ccggcaaaaa agaaggaaat acttactgtt gagcagggca ggccttttat
    10981 gtttattgca ctgtccacca cgcttaacct aaataaacta ctctgggctg ctagcaacca
    11041 ttgtggctta aggttggtta gaaatgccga gttagaacag ctcataggtg tatcctcgtt
    11101 taccgacaga acaagcaaac caggcttaat tgtctctctt tttccaaacc gaagcagcaa
    11161 tggtcttgta gttggccagc taccaaatgt tgactatata gttgaaataa acggaaatgc
    11221 tgcagaagct gaagtaaaaa tcctatccgc ctgtctcagg ggctttcagg gcatacaggc
    11281 agccattacc attaacccat caaaaataaa aataagtgaa cccttttgtc cggagtaaac
    11341 aagttgctgg tctccaaatt ctcagcgcta acccacaagt ccaaggctac caccaacaaa
    11401 acctttgtta aactgtgtta aggtaacacc cttcaaaatc attcccgtta tctttgtttt
    11461 tatgagcaga aaattacttt ggattctaac agtagttatt gccatcgcat ctatcggcct
    11521 gatattccta cagtcgaggt ggataggggt cgccctgcag ataaaggagg agcaatttgt
    11581 tcaaacggca agccttgcca tggagcgaat tattgatgag gttgaaaagc aggaaacagt
    11641 ttttcaggta attgacgaga taaagccata ctactcggtc aacaccaaaa gcagcgccag
    11701 gctagactac cagcaaaaca ttctgaacaa aacaaaaagc gggattcgga ccaagcaaat
    11761 caaccagcag gtgctcacct tcagcagcct tgattctgtg aaaataccct ccatcaccaa
    11821 gaagttatgg gacagcagct tggtgagcat caacaacggc gacctgcata tacccagcct
    11881 atcgaagggt aaaaatttga aaaatattga cctgggactc gatgagaagc tggttaacaa
    11941 gaccattttt gtggagaaca ttgttgacaa gatgattcgg gtggagctgc ccattcagga
    12001 gcgcatttcg caggtacagc tcgactctat tgtgcgccgt gaattgctac gcaagggaat
    12061 aaaagctcgc tacgagtaca ggataaccaa cgaaagggat agcaccattt atgccagccc
    12121 taagtttaag gccgatttca aaggtcttgt gttaaaggat aagcttttcc caaacgattt
    12181 ctttgcacgc aggtatttcc ttacccttta cttccccaac cagaagacat acctgctcaa
    12241 ctcgctgggt cgaatcaccc tttcatcgct ggcgctcacc tttctaattg tcttcacctt
    12301 tagcgtaacc ctttacatca tattcaagca gaaaaaaatt tcggagataa gaaacgattt
    12361 cgtcaacaac cttacgcacg agctaaaaac gcccatctcc accatttcgc tggcagcgca
    12421 aatgctcaac gacaaatcta taccgcttga acgaaaaaat ttagactacc ttggcaccct
    12481 ggttgccgac gagagcaaac ggctcgggtt gcaggtggaa aaggtgctac aaatggccat
    12541 ctttgagaaa gccaagctaa aacttcggct caaggaggtg aacgtccacg aaattatcaa
    12601 gcgggtatcc aacaactttg ccctccagct tcaatccgaa aacggggtgc ttaagcttga
    12661 gctcgaatcc gataaaccgg tggtgttagc cgatgaggtg catataacca acgtggtaaa
    12721 caacctcctc gacaatgccg tgaagtacaa aaagggcaat cccgaaataa ctatttttac
    12781 caagaatagc actgacggga tagtggtaac atttcaggac aacggaattg gcattagccg
    12841 ggaaaacctg cgaaaaatat tcgatcagtt ttaccgcgta cctagtggta acattcacaa
    12901 cgtgaagggc tttggacttg gactgagcta tgtgaaaaaa attgttgagg cacatggggg
    12961 tcgaatttgg gttgaaagca cccctggcga gggtagcctg ttctcaatat ttttgccatg
    13021 gaatggccct tcagaaaaaa agtaaaacca taaaccaaaa tccattacaa tcatggaaaa
    13081 aagaacaatt cgagtgctgc tggccgaaga tgatgaaaac ctggggtttc tgcttaaaga
    13141 gtacctccag gccaaggaat acgatgtaga tctttacaag gatggcgaaa aagcctaccg
    13201 gggttttcag aataactact acgatatctg catacttgac gtaatgatgc ccattaagga
    13261 tggatttaca ctggccaaag aaattaggat ggctaatccc gccatgccca tccttttcct
    13321 tacggccaaa tcgctcaaag aagatgtaat tgaaggattc tcaataggtg ccgacgacta
    13381 catgaccaag cccttcagca ttgaagagct gctgatgcgt atcgaagcca tactccgccg
    13441 cacccgtaag gattcgcccg acagcgagca aagctccttc cagattggcc gctacctatt
    13501 cgacgctacc aagcaaaccc tacagctcga cgacgaggta cgcaagctta ccaccaagga
    13561 gagcgagctg ctaaaatacc tttgcatcaa caaaaactcg ctgctcgacc gtaattttgc
    13621 gctgaaaacc atctgggtgg acgacagcta ctttaacgcc cgcagcatgg atgtgtacat
    13681 cacaaaactc cgaaaatacc tggccgatga tccaaccatc gaaattatca acgttagagg
    13741 aaaggggttc aagcttattt tttagcctgg gtactgcaac caaaaatcta tttacgcata
    13801 tatttgtagc gtaaaatgct tttttggctg ttttttgcat tgtaaaaaaa atattcttac
    13861 ctttaggtag ctgaaattga atgccggtat ggataaaatt attattgaac ccacccttga
    13921 aacacctcgt gttgtgctgg acagagacag ctccataatt gagttttcag gaaattctct
    13981 accggaggat gttaacactt tttacgctcc tattcttgaa tggatcgaca actacgttga
    14041 caatcccaac cagcaaaccg aggtcagcat gaactttgac tactacaaca cctcctcgtc
    14101 aaaaatgata ctccgaatac tcgaaagatt tcgcgacatc caccgtaaag gctatcccgt
    14161 ggttgtcaac tggctttaca tggaggatga tgaggatatg gtggaagccg gtgaggattt
    14221 tgccgaccac ctgaaaatcc ccttcaagtt tatcccaatt cagcactagc agctaaacca
    14281 aaaaccgact caatggctac cccgtcaaaa ttaaaagtat tctccaaccg cctttttggc
    14341 aggttcaaga gcacagaaga ggttgaatcg aatcgcaagg cgcttgaggt cgaatactac
    14401 gaatgggttg agtttgccca atcggatgat tataaacgct ttgaagagct taaaagttgg
    14461 gaagcaacca aggagtacct caccgtaaaa aaggaggttg aggccattaa atactccgaa
    14521 tcgcctgaac aaagggagga gcaggagcta aaaaaacttt cccgcagcca ctccatcaga
    14581 aacttcctca aaatagccaa atccgaaacc cccgattttt accagaaaat ggagcattcc
    14641 cagctggtaa aggaactaaa ggaattgcag caattcattg caactcctga ttatcaagcc
    14701 aaaaaaaatc agtatagaaa agataactca gcagagtacc agaaggaact tagactcaag
    14761 gagttgctaa gtaatcccga tattaaaaag tatttaaaac ttgaaaagga gaatgattta
    14821 aaagagtact ttcaaactga aaattccgaa caactcaagc ggtacaacga gctatccgaa
    14881 aaggttagca ccgaagattt taaaaaccgt aaggcctacc tgcttagtaa gaataagttt
    14941 gagcaaaccg atgcctacaa gaaccttcag gaatttaagc agctggagtc gtcggaaaaa
    15001 ataaaatggt tcttccggct gaaccgcagc aataaatttg atgacctgaa agcatggaac
    15061 ctagcttttt acgatgattt tgactcaaaa aatattgacc cccacaagtg gctcacgaag
    15121 ttcttttggg gcgaagccct tgttggcaaa ccgtactctt ttactaccga caaccagaac
    15181 tacaccgaaa agggaaatat tgaaattggc aacagcaccc taaagattat tacccgcaag
    15241 gaggccagcg aggggctggc atgggataaa aagtttggat ttactccaaa agcatttgcc
    15301 tatacctcgg gtataatcaa caccggccac actttcaggc ttaaggaagg aagaattgag
    15361 gcaaaggttc gtatgagcag tgtgcagggt attagccacg ccttttacct ggtgggcaac
    15421 gagatgcttc cagaggtgga tgtattcctg aaaaataacg ctaagccgag tggcatttca
    15481 gcagcttact tctggcctaa cggcaagagc aaagtagcca aatcggtatc ctccattggt
    15541 gggttaaagc taggcgagaa cttctacatc tttggtgttg aatgggattc gaacaacatt
    15601 gtatggacca tcaacggcat cccgtataag gttgagccca accgccaccc cggtatctcc
    15661 tactacatgg tatttacttc tgcagttaaa ggaagcgttg acgactccct gcttcccgcc
    15721 acccttgaac tcgactgggt aaagtgctgg gagaaaaagg aataacgtta aactctctac
    15781 gcaaatagct accactgcct agccgttacc cgaagcacgg ttttaaagtc ctcatggtag
    15841 gtcatctggg tatcaaactg ccagctccac tccctgatgg tgcaaccggg tattctgaca
    15901 aaaacagttg gattagcgcc aaccaggttt ccatcttcat cgtagtactc ctcctcgtag
    15961 tcggaaacca gcaacgtgcg ccccactgga agctggtcaa catgcctttg ctgaatctgt
    16021 tccgaaagct cgtggagctg ctcgttactc agcttcccct tatgcctgag ataggctata
    16081 ggaatatcgt ttagctggct gagaaggttg agcgaaacaa ccaaatcctc atcaaacgtg
    16141 ggcagggtga tgttcctaat aaagttctcg agttcaaaaa atgtcagctt tcgggcatgg
    16201 cgttgggcta taaaatcgac caggccaccg gtaaggtcaa ccgtttcgaa agatacggag
    16261 ctagcaccgt aaaacctatt aactacctgg ggtgggtgga taatatcggt aagaaggagc
    16321 gtgctgccat gttgtagaat ctcatcaaca ggaacatcga gcaggttgcc gcttccgagt
    16381 atcctcacag ttttggcttt ttgccgcagc aaactttcaa tgattatctg cctggaattt
    16441 tccagatgag ctatccagcc ctgctgctca ctaatgtaac gccgcatgat ggcagcctgg
    16501 tcggatgtat atccaagcct tctaagtgca gagtggctgt tcaccgcagg aatagtttag
    16561 tccattttaa gaacagccag aaaagcctcc tgtggaacct caacggaccc aatttggcgc
    16621 atccgttttt tacccttctt ttgtttttca agcagcttac gcttgcgggt aatatcgcct
    16681 ccgtagcatt ttgccgtaac gtccttccga acggccttaa ctgtttcgcg ggcaataatc
    16741 ttggctccaa tggcagcttg gatggcaatg tcgaactgct ggcgtggtat caagtccttg
    16801 agtttttcgc acattttacg gccaaaatcg taggcatggt caatgtgaat aagtgatgat
    16861 agcgcatcaa ccatctcgcc gttgagcagg atgtcgagct taaccagctt ggcttcctgg
    16921 tatccatcca gaaaatagtc gaaggaggcg taacctcggg aaatggattt aagcttatcg
    16981 tagaagtcga aaacaatctc gctgaggggc aaccggaatg tgagctccac cctatccgag
    17041 gcaatgaaga cttgattctt cagcacccca cgccggtcaa tgcaaagttt aatgacggtt
    17101 ccaagatact ccgacttggt aataacctga gcatttataa tcggctcttc aatgtaatct
    17161 ataacggttg gacctggtaa tccgctggga ttatgcacct caactatttc tcccttggtg
    17221 gtgtgaacac gaaacgaaac gttgggcacg gtagtaataa catccatgtc gaattcacgg
    17281 tacagcctct cctgaacaat ttccatgtga agcagcccaa gaaatccaca acggaatccg
    17341 aagcctagtg caagcgatga ctccggctca aaggtgagag aggcgtcgtt tagcttcagc
    17401 ttttcgagcg atgagcgcaa gtcctcatac tcatcggcat caaccgggta gagaccagca
    17461 aaaaccattg gcttaacatc ctcgaatcct gcaattgcct tgtcgcatgg cgagtcaaca
    17521 tgggtaatgg tatcgccaac cttcacctcc gacgactcct tgatacccga tatgatgtag
    17581 cccacatcgc ccgcgctgat ttggtttcgg ggttgaagct tcagctgaag aattcccacc
    17641 tcgtcggcgg tgtattcaaa gccggtattt acgaacttta ccctatcacc cgttcgaatg
    17701 gtaccgttaa ccaccttgaa gtaggcaata attcccctga aggaattgaa catagagtcg
    17761 aaaactaagg cctgaagggg ggcctcagga tcgcccttgg gaggaggaat acggtttaca
    17821 atagcctcca gtaccgcatc gactccaaaa ccagttttgg cgctaaccgc caaaatttca
    17881 tcacgcttac aacctatcaa gtcaacaatt tgatctttaa catcctccac catagccgaa
    17941 tccatatcaa tcttgttgat gaccggaata atctccaggt catggttaat ggccaggtac
    18001 aggttggaga tggtttgggc ctgaataccc tgcgtggcat caacaaccag caatgcaccc
    18061 tcgcacgagg caatggcacg cgacacctcg tatgagaaat ccacatgacc aggggtatca
    18121 atcaggttga gggtgtatgt ttcattcccg taaggataaa ccatctgaat ggcatggctt
    18181 ttaatggtta ttcccttttc cctttcaagg tccatgctat ccaacacctg atcctgctgc
    18241 tcgcgctccg acagggtgtg agttagctcg agcaacctgt cggccagggt actttttcca
    18301 tggtcaatat gggctatgat gcaaaagttt cttattttct ccatgcacat ttactgttga
    18361 agcgcaaagg tattcaaaaa aaatggatat gcctatgagc caccgctatg atggcaacaa
    18421 atcaaaaaat acattaccta atgcttctcc agcaaaccaa cttgcatggt gggatgcaat
    18481 caggccgatg gctgctgctt cgtatccctt ttatccggct ggtttagcaa tttcatggcg
    18541 aactccttaa cccggtttaa atcatcggga gtatcaacgg cgagcgtttc ctggtcggtt
    18601 tccagcacac gaatggtaaa tccgttctca atccatcgta gctgttcgag cgattcggcc
    18661 aactccagcg atgattgggg caagcgggtt atttgcctga ggatatcggg tcggtaggcg
    18721 taaagcccaa tatgcttaaa gaacctatgg gccagctgcc attcggcctg agccttcccc
    18781 ctgacgaagg ggatagccga acggctgaag tatatggcat tgccctctgt gctaacaact
    18841 accttaggag cgtttgaatt aaaaatatct tcattactac caaaggcctt aaccagcgtg
    18901 gcaatttgaa ccgttctgtc gctaaagcac tctgtaattc tctgcagctg ctccggttga
    18961 ataaaaggct cgtcgccctg aacattgaca actacatcaa acgaaatatt taggctattc
    19021 tggagtattt ccattgcttc ggcacacctg tcggtcccgc tgcgatggtg ttcggatgtt
    19081 ataaccgatt gtccgccaaa accctgaaca acattaacaa ttctttgatc atcggtagcc
    19141 acgtaacaat ggtcaaaaac acctgatgct ctttcgtaaa cccattgcac cattggcttt
    19201 cctgcaataa ttgccagagg cttacccgga aaccgggtgg aggcatacct ggcaggtatt
    19261 agtcctaaag ttttcattca ctcgggagta aaattttaca gaacaaataa tgtaatgtta
    19321 tccggccgat taaattaccg caacacattt taaatgtagc tttaccatta tgcttacaat
    19381 ggccctcgcc ggttaaccag cgaaaaggtt caacaaaaat aagtaaaaac aaagcacata
    19441 catactgcaa cagctcttat gctcagaaac tcctgttgaa gcagtcacat aatacaagta
    19501 atacaagtat aaatgtatac ctgagtttta gcattaagtc aaaagaattg ctctaccaaa
    19561 actggcagca tcggaataaa aacattcaat gcgtggctat tccggagtaa cctggtaagt
    19621 aagaaaaata atccgatatg ccgaattata gggaatataa gaactcctgt aaacttttag
    19681 aacatagatg tataggcaat aaatgtttca gccaaaaagt ttatcattct aaaaagtcta
    19741 aaaaatagct cgggttactc cattttttgc taactatgcg gcaaaattca ggcaagatgt
    19801 cccatcaaaa cgagtcggtt aaatctattc tgctagccct tttggcgaac ctgggaattg
    19861 caattaccaa aacgattgcg gcagtagtaa ccaaatcggg tgcgatgatg gcggaatcca
    19921 ttcactcatt cgccgattgt gccaaccagg ggctgctatt ctggggttta aaggcatcca
    19981 gaaaaaaacc caatcccgaa catcctttag gatacggaaa ggaaatatac ttctggtcgt
    20041 ttatcgttgc cctgatactg ttcagcatgg gaggactctt ttcggtttac gagggcattc
    20101 ataaggttag cgcacacgaa gggctaagga ataccaatgt ggctataatc gttctcgtgg
    20161 taagcatggt tttggaatca atctccctat acggttgctt aaagcaaata cgtaaggtta
    20221 gacatggaga atcgttgtgg aaatggtata aaactagccg ccagagcgag ctggtagttg
    20281 ttctaagcga agatattgca gcccttctag ggcttaccct ggcgctcgtt gcggtaatac
    20341 tatctattgc aaccggcaac cccatttatg atgcagcagg tagcattggt ataggcattt
    20401 tgcttgtgat agtttcctgc tttttggcag ttaaggtaaa aggtttactg attgggcaaa
    20461 gcgtggatga cgaaaccagc aataaaatca ggaaactaat cgaatcaaga ccagaggtag
    20521 aaaagatatt caacctgatc accattcaac ttggaccgca ggtaatggtt gctgtaaaag
    20581 caaaaatgaa agctacagat aacataaatc agctcattag caatattaat gcatgtgaag
    20641 aagcgcttac gcaggaaatc tcaaccattc aatgggtatt ctttgaaccc gacttaaagg
    20701 attaacactt gtgctcacaa aaaaaggtat gcgttagttt ctataaccgg gcttggtggg
    20761 gctgaacagc cggttaaatg gtagtaaacg atgtgcgtaa aatcaacagg gaacaaacca
    20821 tccgtgccta agaggacatc tcatacactt aaaatttttc acatttattt gtaaatttca
    20881 attgggcagt atatttttgc aaggcaattc aacagtagag aagactatta tttgaaagtc
    20941 ttgcggaa
//
LOCUS       DAOPHX010000031        20850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_2721_length_20850_cov_5.738735, whole genome shotgun sequence.
ACCESSION   DAOPHX010000031 DAOPHX010000000
VERSION     DAOPHX010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 20850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20850
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2721_length_20850_cov_5.738735"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PK502_03800"
     CDS             complement(<1..213)
                     /locus_tag="PK502_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99973.1"
                     /translation="MRNKKVLRLGLLGLLATMMVASCSKEEDERLQEPISNDWTVRPD
                     FATLDTRPQDVIASFTLTEKDAELDVL"
     gene            complement(609..1856)
                     /locus_tag="PK502_03805"
     CDS             complement(609..1856)
                     /locus_tag="PK502_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99974.1"
                     /translation="MIKYAISKADQNLKGEITLSPSKPMNHKFLVIRILKSSNLTQRL
                     TTESEDAQIVNSEVINEGIKKNRGTSAKALRHIRAFLNYFGGEWILSSSNILKDRSTE
                     RIVKVLQKSGLNVSYEKRSGRPPFRLTGKNLSGIIQRVDGSINSKIIESKLLFAPTAS
                     IDQIEEVCNRILSSNYVGTTLRALQYLGVNTDWKEDEVLVEHEINDGSEMVIEPDWSL
                     ASCWYQMVALAQKGELQINGLKIDTFQNESAIRHIYNDFGISTLIMPDGISIKRKGKL
                     AGKFTHDFTSYPNLLPCAITTCVGLGIPFEMKGIESLNAADPNRMQLLTEELVKVGAK
                     VELKSINGTQVATFNGTSGLSRLKSVEFESKNDYRILLALVSIAVRGIQVIIDSPMAT
                     NKAYPSFWDDMKKLGFVVKSVTE"
     gene            complement(1960..2745)
                     /gene="cdaA"
                     /locus_tag="PK502_03810"
     CDS             complement(1960..2745)
                     /gene="cdaA"
                     /locus_tag="PK502_03810"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HPG99975.1"
                     /translation="MTNLFITIRALDVIDVVLVAFLLYQMYMLIRGTVAINIFVGIVL
                     LYIFWLVVRALNMELLSSILGQIIGVGVLALIIVFQQEIRKFLLFIGNRYINRRRFSF
                     ENFFFPKKREKQILVKVDAIVKACRNMSETRTGALIVIARKSELQLYAESGDIIDADT
                     STRMIENIFFKNSPLHDGAVIIVNDRILAARCVLPITENINLPPHYGMRHKSAIGITE
                     VTDAIAIVVSEETGKISVASEGMLRYDIGWLELDKYLRDNLSG"
     gene            complement(2821..3702)
                     /gene="folP"
                     /locus_tag="PK502_03815"
     CDS             complement(2821..3702)
                     /gene="folP"
                     /locus_tag="PK502_03815"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPG99976.1"
                     /translation="MEQPPSYLKQRKTMTSNGRIISLDKPLVMGIINVTDDSFYSGSR
                     YKLGFRIAARAEQIIREGGSIIDVGACSTRPGAKPVSEQQELKRLAKALHAIRKKYPE
                     AIISVDTYRSRVARMAVGDYRANIINDISAGELDPEMFSTIAELKIPYVLMHMRGTPE
                     TMQQNPTYDNVIREIITYLNGKIEQLKQLGVSDIIVDPGFGFGKTVDHNFSILKNLQV
                     FRLFERPILVGLSRKSMISKTLGIPAAKCLNGTTVLNTMALLNGATILRVHDVREATE
                     AIKLVESTTRQPDYQYI"
     gene            3805..4497
                     /gene="tmk"
                     /locus_tag="PK502_03820"
     CDS             3805..4497
                     /gene="tmk"
                     /locus_tag="PK502_03820"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPG99977.1"
                     /translation="MALIVIEGLDGAGKSTQINLLGSFLTQKGVASHFLHFPRVDAPY
                     FGELIARFLRGDLGRIDEVDPYVVAMLYALDRMDASGLIKSWLKTDEVVLLDRYVYSN
                     IAFQCAKLNDSSRVDKLRSWILNLEFDYFAIPKPDISIFLDVPFEFTSKRLSEQREGA
                     DREYLNGRDDIHEKDLNFQQRVREVYLLQQSLDPQFVVIPCYDEHGSMLEPDKIFNRI
                     VEAILVHKALVI"
     gene            4523..5740
                     /locus_tag="PK502_03825"
     CDS             4523..5740
                     /locus_tag="PK502_03825"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPG99978.1"
                     /translation="MKQVVIISRYLLAVVFIFSGFVKGIDPMGSAYKFHDYFMAFHMN
                     FMQPLSLAFAFILCAAELLIGLMLLYGVNLRIAAWGVFIFMLAFTPLTLILAIFNPVS
                     DCGCFGDALHLTNWQTFFKNIVLLSAAYTFFRYRASLPPSFGEKKQSAMFAVLCVVAF
                     LPPIHGSLHEPLYDFRPFHVGVNIHQAMIYPDGAPADVYKTKLYYKKNGEVKEFDESN
                     YPWQDSSWTFVDSKFILVKKGYTPPISNFSLHSVDGVDLTDTIIQSTGYTFLAVSPQL
                     EKAGVKAQEQVKNIFMGATERGYKFYWATASSGEAMEKIKVNSRVELPFVLGDEVTLK
                     TITRSNPGLILIYNGTIIGKWGWRDIPEQLFKNPDLLAWQLNNQRLIAGNRLTATLVL
                     MLMLAFIALKPWK"
     gene            5873..6310
                     /locus_tag="PK502_03830"
     CDS             5873..6310
                     /locus_tag="PK502_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG99979.1"
                     /translation="MGVKQLCSVWLILLANIMLQVHAFVPHHHHNGQVCFDGYSVNIT
                     IPNDGNDASNNRAHPTDEDAECCALKHDYLIPDDGFEYKPAVTNIHHGFNIAGGFITS
                     CASIPFQIFLVNTRLYGGYTNNFHFSSYVSIPGLRSPPAKNLL"
     gene            6534..8060
                     /locus_tag="PK502_03835"
     CDS             6534..8060
                     /locus_tag="PK502_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPG99980.1"
                     /translation="MKRLFILIFSITIYGCSHTADSSAGAAHQHDESSHEAEAGDDAQ
                     TFTIYTSTCELFAEAKPFVVGTTTTVLSHFTNLADFKPLTSAAITIKLTVNGKEYSQT
                     IAIPEKNGIYHFTVTPDLSGQGQLVWIVERDGKSELFDAGLVRVFANEHEAKAFHAEH
                     IPADAIRFTKEQSWQVDFSTEWPCMEAIGEVIKTTARVEPAPTDEMVISAKTGGIVSF
                     YSSKPLVLGQHVERGDKLLEIVSSGLANEDLSVRYAEAKSNFERAKSNYERILELSKS
                     KVASEKELGIALNEYQNASSTYTNLKENFSASGQNVSSPIDGYVQDISVQNGEYVEAG
                     QPVLTVFGSRFYVLNAGVQQKYFKSLEGLKTATVKQANGSYVGIDKFNGRIMSVGNSA
                     LAGNGYLIPVIIQVDATDGLVVGSFVELNLIAKSDSMLLTVPTLAILEEQGNYYVLVQ
                     LNPETFEKRQVQLGVSDGLRVEIRSGLLSTERVVTRGAVLVKLAQVSGKLDAHAGHVH
                     "
     gene            8072..11173
                     /locus_tag="PK502_03840"
     CDS             8072..11173
                     /locus_tag="PK502_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPG99981.1"
                     /translation="MLDRIIKFSLSNKYTILIAFFVLAVVGIRTARNMDVDVFPDLTA
                     PTVVVMTDAHGMASEEVERLVTFPIETAINGATDVRRVRSVSSQGFSFVWVEFDWGSD
                     IFKARQIVSEKLITVSSQLPIGVGQPVLAPQSSVMGEIFFVGLTADSTSMMDLRTIAE
                     WEVKPIILATNGVSQVTIIGGDFKQYQVLADPVKMMSYGVSMDELATVCRNISGNSTG
                     SNIRQFGNEYAVRGMARASDLETLGNSFIKFLNGHPVRVSDVAEVVIGSAAKMGYASE
                     NTKPAVIISISKQPNANTLKVTENIEKNLDNIYKTLPKDVRLDTGIFRQADFIQTSVG
                     NVQNALMEGAIFVVIVLFLFLGSFRTTIISLLAIPLSLLGTIVALKAFGLTINTMSLG
                     GMAIAIGSLVDDAIIDVENVFKRLRQNNLKPVDERQSSFTVVFEASREIRASILNATL
                     IIIVAFLPLFFLSGMEGRMLKPLGISFIVSLTVSLVVAMTLTPLLSKMLLTNESYLAK
                     NEREKWLSRNLQTYFKASLEWVLRHKKVVLGAVLSLVILSIVAFASMGRSFLPEFNEG
                     SLTLSVVTKPGTSLDETNKLGNLVEQQLLAIPEIYSTARRTGRGELDEHSQTVNSSEI
                     DVNFKLSERSRDEFVANVRQTLAGIPGIAATVGQPLGHRIDHMLSGTRANIAIKLFGT
                     DLNQMFAIGNQIKGEISGIEGLVDVNVDQQVDIPQIQLKANRDMLAAYGIPVGSFNEF
                     IDVAFGGEKLADIYEGQRRFDLVLKLNHRYTESIEGIGVALIDTYNGGKVPLSEVAQI
                     QSVSGPSSISRENAQRKVVVSANVAGRDMRSVVNDIQDRVNSHITLPEGYRIEYGGQF
                     ESEANASRTLMITSILAILVIFLLLFQEFRNFKLAAIILLNLPLALIGGIFSIYITGG
                     ILSIPAIIGFITLFGIATRNGILLVSKFQQLQAQGLPIHDTILKGSTDRLNAILMTAL
                     SAALALIPLAMRGHLPGNEIQSPMAKVILGGLITSTILNIYIVPIAYSLLAKRGLTKT
                     DKQ"
     gene            11170..12348
                     /locus_tag="PK502_03845"
     CDS             11170..12348
                     /locus_tag="PK502_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPG99982.1"
                     /translation="MRRISLIISNLLLFVAIANAQVSIEKILGEVERNNTTLVALRQK
                     MEAEKIGSRTGLYPQNPSFEYSYQWGNPADIGSKTDISLIQSFDFPTAYLYRSKIAKA
                     EIELLATEYLRQSKQLLLDVRLVCIELVYQNALSSVLGKRVSNAKLIADAYSKKLDVG
                     EASALENNKARLTLLNATQELFSVKLKQNELLAKLASLNGGIPLELSDSVFNMPAIPA
                     SFDEWYAATEQSNPDLKAIEREIAVAQRNIQLSRALCLPQIKAGYASEKVAGEHFQGI
                     AAGISIPLWENSKRVKYAKARAQYLRSVEADRKVEIFSRQQAQYNTALELQKNIAEYR
                     NSMGTINSSRMLDEALQKGEISLVDYLLEESLYYSSAETLLQMERDFCRVVAELYQYQ
                     "
     gene            complement(12654..>13047)
                     /locus_tag="PK502_03850"
     CDS             complement(12654..>13047)
                     /locus_tag="PK502_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPG99983.1"
                     /translation="NLNSETTYIAFVVDGNLYNIDYWYVDNVSIVASGSGSPAFDTPI
                     SDLTENDGVKIFNLYPNPASDYINISLTGASSEVTVRIFDVRGSMVKSQVLNASENRI
                     DVSTLAKGIYMVSVGEGKEISTKRFIKQ"
     assembly_gap    13048..13147
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13148..15947)
                     /locus_tag="PK502_03855"
     CDS             complement(<13148..15947)
                     /locus_tag="PK502_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HPG99984.1"
                     /translation="MKKTLLSFLGVCLMAAAMVTNQAVAVPAYPGLIKYEQANGKIIN
                     IYLKGDEKVKWVLSEDGYTLMLNSKGVYEYAFQDGKGDITLSGVEANNPAERTKAERE
                     FLGKVSKGLYYSPSQLSLMKSIWEIKSAESLKAFPTTGNRKLICILMGFKDKAFTKTQ
                     SDFNNLFNQVGYTTGGATGSVKDFYMENSWSQFNLTVDVFGPYTSSQNMSYYGANDAS
                     GYDVNPRALVTEAVNAADASANYANYDNDGDGTVDGVYVIYAGYGEEAGASADCIWAH
                     AWSITPVTKDGKTISKYSCSSELRGTSGTTITAIGVICHEFGHVLGAPDYYDTDYSTG
                     GQYEGTGYWDLMASGSWNNNGVTPAHHNAYTKVKVYGWATATVLSSAATISVTNAEAN
                     KSFYQINSTTTNEYWIMENRQQVGFDANIPGHGLLIYHVHKDIATASSSNNINATYPQ
                     KMYPVCASATTNPGSTPSSYGSISSTGCPWPGSSSKTSFTDATTPNMKSWAGANTAKP
                     MTNIAENTSTKTVTFDFMGGSGGGTAPTATTLAASAISTSSATLNGQVNANNATTTVT
                     FEWGTTTSLGSSFSATPSSVTGTTTTNVTGNLTGLSANTTYYFRVKAVNASGTTYGST
                     LNFTTSSTPTSITLPVTENFNSSTLPTGWTTQNTGTSITERWSVSNTYKAGGSAYEMK
                     CTYQNVNPGTTRLITPAINTVGVSQVTLSFKHMLDAYGSGATLRVQTSNNKSTWTNTT
                     WSVATGSSNVGPATVTVNITSNLNSETTYIAFVVDGNLYQIDYWYVDNVSITAGSGAT
                     IPTVTTNSVTSITSSSASCGGNVTSDGGATVTERGICYSTSQNPTTSSSKVTSGTGTG
                     SFTANMTGLSASTTYYVRAYAINAQGTAYGAQQSFTTLSGSTSLPLPVTQTFNTSSLP
                     TGWTTQNTGTSITERWSVS"
     gene            complement(16385..16804)
                     /gene="ndk"
                     /locus_tag="PK502_03860"
     CDS             complement(16385..16804)
                     /gene="ndk"
                     /locus_tag="PK502_03860"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPG99985.1"
                     /translation="MNTRFTFCMIKPDAFKNDCVSEILCEILKAGFKVRGIKITMLTK
                     AKAEQFYDIHRGKDFFDKLTTYMSSGPVMAMVLEKPEAVEALRKLVGKTNPEEADEGT
                     IRRRFGVNTTQNAVHASDSYENAEHEWGFFFSKREIY"
     gene            17103..18137
                     /locus_tag="PK502_03865"
     CDS             17103..18137
                     /locus_tag="PK502_03865"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPG99986.1"
                     /translation="MKLNLDEAWAGALRELLVPTNRIVLTTHHNPDGDAVGSVLGLWH
                     ILEQVGVHATAVTPNDFPDFLSWMPGSDTILRFSSSRQKVREVMAGADIIICLDYNGF
                     SRTEDMESLFSASSAKKILIDHHPQPENTFDLSISVVEVSSTSELVFELATEIYGLKA
                     LNADAATCLYAGIMTDTGSFSYACSRGRTLEIAGMLVDSGAVVERIQSLVYNNFSADR
                     MKLLGYCLADKMMVFPEFHAAYIWLTREELKRFNYQMGDTEGLVNYPLSIKGIIFSVI
                     FVENDNHIKVSLRSRGNFSVNEFARKYYNGGGHLNAAGGKSFQPIDATLADFEQLLRQ
                     HLQELNAAAI"
     gene            18172..18696
                     /locus_tag="PK502_03870"
     CDS             18172..18696
                     /locus_tag="PK502_03870"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPG99987.1"
                     /translation="MKILTIIPLAVILVSCGGNERQPDRQPNVAETREMLQRINKVLV
                     QQDREQILDYIKRNNLVEMKESPTGLFYYISGTSNGPKVRKGDVVEYSYRVSLLDGTV
                     CYQSNAEAPATFEVGHGGVVSGLEEGILLMRQGQSAKFIMPPHLAYGLIGDSKQVPAR
                     SIVIYDVELLAVYR"
     gene            18743..19630
                     /locus_tag="PK502_03875"
     CDS             18743..19630
                     /locus_tag="PK502_03875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPG99988.1"
                     /translation="MTKSSGILLAFLVVATVGCSNRSKLFPGFSVTPTGIHYKLISLG
                     NGTSTAKPSNYITASISYRTAKDSVFFQGTRQFQLAQSSYEGSIDECFMMLAEGDSGA
                     FYIKALPFFTQTLETKLPTFIGVNDYMRVDIRLHSIKTQEEFKKDMEAFLSWIEDFGD
                     YEKVIIRQYIDGQKLSVEPTPSGLYIIPQIRTERAPVEKGDTITIHYEGRFFDGRIFD
                     STRKRNEPFQLVYGDKWQVIPGIDEALGTMREGEKSMLIVPSHLAFGQQGNTSGIIPP
                     FTSLVFELEVVEVKKGVQL"
     gene            19627..20523
                     /locus_tag="PK502_03880"
     CDS             19627..20523
                     /locus_tag="PK502_03880"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPG99989.1"
                     /translation="MIQKNFFNAILLLSPLLMGACDNSLKEDKRNDEEKIIEGLINSK
                     KWTYTKSEGVYHVVVQPSYGYEVNYGDTVTFWYKGYTIQSPVLVFDTNIKSEAVLAKL
                     DTNTRTLEPVRVIAGKTKLIEGLKRGLLLCRQGQKSTIFFTSDIGFGDNQVGPLPPWT
                     PLAYDIEVVALNGRGIESEQNIIKSMDLTGYTLHSSGLYYRYLLDSSTVRPLSNSVVY
                     GWYSISLTDGTVVTQTPGANEKVDLTGQMPEALRLGFLLASLGGKITILAPSPLGYGK
                     SGSSKVMPYVPIVVDIRLDSIK"
BASE COUNT         5323 a         4445 c         4941 g         6041 t
ORIGIN      
        1 cagtacgtcc aattcagcat ccttttcggt gagggtaaag ctggcaataa catcctgtgg
       61 acgggtatct agagtagcaa aatccggacg tactgtccaa tcgtttgaga ttggctcctg
      121 caaacgttca tcttcttcct ttgagcagct agctaccatc atggtggcaa gtaggcctag
      181 taaccctagc cttaacactt ttttgtttct catagcactt ttaatttgtt ggtttggtta
      241 attggtttga gttgattttt tgttgaaaat gaaattcaac atccgaaagt taggttgatt
      301 acgcaaacaa aagctgattt tgcgacaaac gacggcttgc tacgcacgaa taaccggaat
      361 cgctgcatat aaaacaaaat ggtaattttt agagtaaaaa tacctgcagc ttaccggaag
      421 ttatggtgga gttggtgtaa tggccgttag ccggcttcca tgcatggcaa agtagcgggt
      481 agccaaccta cctgctgtta aaaaattcta accgacgcat acagcaatga gcctgccttt
      541 tatgacgaaa acgcccgggt ttttaagggt tgatttgaca gcccaagggg ttctctgaat
      601 aatagctatt attcggtaac actctttacc acaaacccta gttttttcat atcatcccag
      661 aaactagggt aagctttatt ggttgccatc gggctgtcta tgatcacttg aatgcctctt
      721 actgcaatag agacaagagc aagtagtatg cggtaatcgt tttttgactc aaactcaaca
      781 cttttaagcc ggcttaggcc tgaggttccg ttaaaggttg ccacctgggt tccgttaatg
      841 cttttcagct ctacttttgc tcctaccttg acaagctctt ccgtgaggag ctgcatcctg
      901 ttggggtctg cagcattaag gctttcaatt cccttcatct caaatggaat acccagacct
      961 acacaggttg taatagcgca tgggagaaga ttgggatagc tggtaaaatc gtgggtaaac
     1021 ttaccggcca gcttaccctt tcgcttaatg ctgatgccgt cgggcataat tagggttgaa
     1081 atgccaaaat cgttgtagat atgccgaata gccgattcgt tctggaatgt gtcaattttc
     1141 agcccgttaa tctgcagctc acccttttgg gcaagggcaa ccatttggta ccagcacgaa
     1201 gctaacgacc agtcgggttc aataaccatc tctgacccat cgtttatctc atgctccacc
     1261 agcacctcgt cctccttcca atcggtgtta acacccaggt actgaagtgc ccgaagggtg
     1321 gtaccaacgt agttggagct aagaattcta ttacacacct cttcaatttg gtcaatgctg
     1381 gcggtagggg caaatagcag cttgctctca ataattttac tgttaatgga tccgtcaacc
     1441 cgttgaatga tcccggaaag gttcttgccg gtaagccgga aaggcggtct gccgctacgc
     1501 ttctcgtagc taacgtttaa accgcttttt tgaagaacct taacaattct ctctgttgat
     1561 ctatccttaa ggatgtttga tgatgagaga atccactcgc cgccaaagta gttgaggaag
     1621 gccctgatat gccttaaggc ttttgccgag gttccccgat tctttttaat tccctcgttg
     1681 attacctcgc tgtttactat ttgtgcgtcc tccgattcgg tggttagtcg ctgggtaagg
     1741 ttggagcttt tgagaatgcg aataaccaaa aacttgtggt tcatgggttt ggagggagaa
     1801 agggtaattt cacctttcaa attttgatcg gctttcgaaa tggcatactt aatcatggtg
     1861 catcgaatta aattatgata tggtcccagc ggtaaatgta agtaaaatga agctaattaa
     1921 tgcaagctac catatcataa ttcatgaggc agttgattat cagcctgata ggttatcgcg
     1981 aaggtacttg tccagctcca accagccgat gtcgtacctt agcatcccct ctgaagctac
     2041 gcttatcttt ccggtttcct ccgaaaccac aatggcaatg gcatcggtaa cttcagttat
     2101 tccaattgcc gatttgtggc gcataccgta gtgaggtggc aggttgatgt tttctgttat
     2161 gggaagcacg caccgtgcgg ctagaatccg gtcgttaaca atgataactg ccccatcgtg
     2221 cagcgggcta ttcttaaaga aaatattttc gatcatccgg gttgatgtgt cggcatcaat
     2281 aatatcgccg ctctcggcgt aaagctgcag ctccgatttt ctggcaatca caattaacgc
     2341 accggttcgg gtttccgaca tgtttcgaca agccttgact atggcatcaa ccttaacaag
     2401 aatttgcttc tcgcgttttt ttggaaagaa gaagttttca aacgaaaatc gccttctgtt
     2461 aatgtaccgg ttgcctataa acaggaggaa ctttcttatt tcctgctgaa acactatgat
     2521 cagtgcaagc actccaacac cgataatttg tcccagtatg ctgctcagaa gctccatgtt
     2581 gagagctcgt actactaacc agaatatgta gagcagcaca atgccaacga aaatgtttat
     2641 tgctacggtt ccccgtatga gcatgtacat ctggtagagc aaaaatgcca ccagaacaac
     2701 gtctatcaca tcgagtgccc taatggttat gaaaaggttg gtcattggca aatagattat
     2761 tttaaagtct ggcgctcggt tacaaaataa tgtaagtaga gtgttgtggc atgagttggc
     2821 ttaaatgtac tggtaatcgg gttgacgggt ggtgctttcg accagcttaa tagcctcggt
     2881 cgcctcccga acatcgtgaa ccctcagtat ggttgcaccg ttcagaagcg ccatggtgtt
     2941 aagcaccgtt gttccattga ggcatttagc tgctggaatt cccaatgttt tgctaatcat
     3001 cgatttgcgc gatagcccta cgagtatggg tcgctcaaaa agcctgaata cctgtaaatt
     3061 tttcagaatg gaaaagttat ggtcaacagt tttgccaaaa ccaaaacccg gatccacaat
     3121 aatatcgctc accccaagct gctttagctg ttctattttt ccgttcagat aggtgataat
     3181 ctcccgaata acattgtcgt atgtagggtt ctgctgcatg gtttcggggg ttcccctcat
     3241 gtgcatcagc acatatggaa ttttgagctc ggcaatggtg ctgaacattt cagggtcaag
     3301 ctctccagct gaaatgtcgt ttatgatgtt cgcccgataa tcgccaacag ccatacgggc
     3361 aaccctcgat ctgtaggtat caaccgaaat tatagcttcg ggatatttct ttcggattgc
     3421 atgcagggct ttcgctagcc gtttaagctc ctgctgctcg ctaacgggtt tagctcccgg
     3481 gcgagtggag catgctccta catctataat gcttccaccc tcacgaataa tttgctcggc
     3541 gcgtgcggct attctaaacc ccagcttgta gcgacttcca ctatagaatg aatcgtcggt
     3601 tacattgatt attcccataa ccagtggttt atccaagctg ataattcggc cattgctggt
     3661 catggttttc ctctgcttga gatacgacgg cggttgttcc attttttgct atattgcggc
     3721 ttgttcgatg gctaaagtta ctaaacattg atgtaatttt gccacagcaa gttgattttt
     3781 gctgcaaagg taaacatttt actcatggca ctgattgtta ttgaaggtct cgatggcgct
     3841 ggtaagtcaa ctcaaataaa cctgctgggt tcttttttaa cccaaaaggg tgttgccagc
     3901 catttccttc attttcccag ggttgacgca ccatactttg gagagctgat tgcccgtttc
     3961 cttcgaggcg atttgggtag aattgatgag gttgatccct atgtggtagc catgctttat
     4021 gcgcttgacc ggatggatgc gtcgggttta attaaatcgt ggctcaaaac tgacgaggtg
     4081 gttctgctcg accgctatgt ttactccaac attgcctttc agtgcgctaa acttaacgac
     4141 agcagtaggg ttgataagct aagaagctgg attttaaacc ttgaatttga ctattttgcc
     4201 attccgaaac ccgatattag tattttttta gacgttccat ttgaatttac cagtaagcgc
     4261 ctttccgagc agagggaggg cgccgatagg gaatacctta acgggaggga cgatattcac
     4321 gaaaaggatt tgaatttcca gcaacgggtt cgagaggttt acctgctaca gcaatcactt
     4381 gacccacaat ttgttgttat tccttgctat gacgaacatg gctcaatgct ggagccggat
     4441 aaaattttta acagaattgt tgaggccatt ctggtacata aagcattagt aatttgacat
     4501 ttaaattgaa aagtttctac ttatgaagca ggtcgttatc attagcaggt accttttagc
     4561 cgtggtattt attttttcgg gatttgtaaa aggcatagat cccatgggtt cagcctataa
     4621 gtttcatgac tactttatgg cttttcacat gaattttatg cagcccctgt cgctagcttt
     4681 cgcctttatt ctttgcgcag ctgagctgct cattggctta atgttgcttt atggtgtaaa
     4741 tcttcggatt gctgcatggg gtgtttttat attcatgctt gcctttacgc cccttaccct
     4801 tatcctggct atttttaatc cggtttcgga ttgcggctgt tttggcgatg ccctacacct
     4861 gaccaattgg caaacatttt tcaaaaatat tgtcctcctg tcggctgcat atactttctt
     4921 caggtaccgg gcgtcacttc ctccttcatt tggcgagaaa aaacagtctg ctatgtttgc
     4981 tgttctctgt gttgtggctt ttcttccccc aattcatggt tcgctgcatg aacccttgta
     5041 cgacttccgc ccatttcatg tgggtgttaa cattcatcag gcaatgatat atcccgatgg
     5101 tgcccctgct gatgtgtata aaacaaagct ctactataag aaaaatgggg aggtaaagga
     5161 atttgacgaa tctaactatc catggcagga ttcttcgtgg acttttgttg attccaaatt
     5221 tatactcgta aagaagggtt atacaccgcc catttcgaat ttctcccttc attcggttga
     5281 tggagttgac ctaaccgata cgattattca atcaacgggt tatacttttc tggcagtttc
     5341 cccacagctc gaaaaggctg gagtaaaagc tcaggagcaa gtgaagaata tatttatggg
     5401 cgcaaccgag agggggtata aattttactg ggccactgcc tcttcgggag aagcaatgga
     5461 aaaaattaag gtaaacagta gggttgaact tccatttgta ctaggtgatg aggttactct
     5521 gaaaaccata accagatcga accccggtct aattcttatt tacaacggga caattattgg
     5581 gaagtggggt tggcgcgata ttcctgagca actttttaaa aatcccgatt tacttgcatg
     5641 gcagctaaac aaccagcggt taatagccgg aaatagactt actgctactt tggtgcttat
     5701 gctaatgctg gcatttatag cacttaagcc ttggaagtag cttactttta aactccacgg
     5761 gtgaacttaa aacatataaa aggataaagg gtaaagaaaa cttcttacca aattaaacct
     5821 taccttttgg ttttcatcga aattataaag attttttaat ctttacccaa cgatgggagt
     5881 gaagcaactt tgttcggttt ggctaatcct gctggctaat ataatgctac aggttcatgc
     5941 ttttgtaccc catcatcacc acaacggtca ggtttgcttt gatggatact ccgtaaacat
     6001 tacaattccg aacgatggaa atgatgcaag caataaccgg gctcatccta ccgacgagga
     6061 tgctgaatgc tgtgcgctaa agcatgacta cctgattccc gacgatgggt ttgaatataa
     6121 acctgcggtt accaatattc atcatggctt taacatagct ggtggcttta ttacttcttg
     6181 tgcttcaatt ccttttcaaa tatttttggt aaacacaagg ttgtatgggg gttatactaa
     6241 caattttcat ttttctagct acgtaagcat tcctggccta cggtctcctc cggctaaaaa
     6301 cctcttatag cctaatcttt tccaaccacc taaatggtgg atcttcctgt aatttactca
     6361 agtttaagtg actgtttgtg aatcagctgc gtttcgacgt ttagctgctt gcaggtaaat
     6421 agttcatgga ggatgatttc aaaaaaaatc tttaagtttt cctgcattca acgtaaacgc
     6481 ttgaaatgca aaaatatacg taattacagt gtaacttaaa attttatata cggatgaagc
     6541 ggttattcat tctgattttt agcattacaa tttatggctg tagccacact gccgatagca
     6601 gtgctggtgc agcccatcaa catgacgaaa gtagtcatga agctgaggcc ggtgatgatg
     6661 ctcaaacctt taccatttat acatcaacct gtgagctatt tgccgaagct aaaccttttg
     6721 ttgttgggac aactactaca gtgttgtccc attttactaa tcttgccgat tttaaacctc
     6781 ttacaagtgc ggctattacc ataaagctga cggtgaatgg taaagagtac tcccaaacaa
     6841 ttgccattcc tgaaaaaaat ggtatttacc attttacggt tacacccgat ctgtcaggac
     6901 agggacagct ggtgtggatt gttgaaaggg atggcaaaag cgagctcttt gacgcaggct
     6961 tggttagggt gttcgctaac gaacatgagg cgaaagcgtt tcatgctgaa catatacctg
     7021 ccgatgcgat tagatttact aaggaacaat cgtggcaggt tgacttttca accgaatggc
     7081 catgcatgga agccataggc gaggtaatta aaactaccgc aagggttgaa cctgcaccaa
     7141 ccgacgaaat ggttatttcc gcaaaaactg gtggtattgt atctttctat agcagtaaac
     7201 ctctggtgct agggcagcat gtcgagcggg gtgataagtt actggaaatt gtaagtagtg
     7261 gtttggccaa tgaggatttg agcgttaggt atgccgaggc aaaaagcaac ttcgagaggg
     7321 ctaagtctaa ctacgaacga attctcgagc tttctaaaag taaggtggct tctgaaaaag
     7381 agctgggcat tgcactaaac gagtatcaaa acgcaagttc aacatacacc aacctaaagg
     7441 agaactttag tgcatcggga caaaacgttt cttcgcctat tgatgggtat gtgcaggaca
     7501 tttccgttca aaatggagag tatgttgagg ctggtcaacc cgttttaaca gtttttggca
     7561 gcagatttta tgtgctcaat gccggagttc agcaaaaata tttcaaaagt ttggagggtt
     7621 taaaaaccgc tacggtaaaa caggccaatg gcagctatgt tggaatagat aagttcaacg
     7681 gcaggatcat gtcggtaggg aattcggcct tggctggtaa tggctacctg attccggtga
     7741 ttattcaggt agatgctacc gacgggcttg ttgtgggaag ctttgttgag ctaaacctga
     7801 ttgccaagtc ggatagtatg ctgctgactg tacccacatt ggccattctc gaagaacagg
     7861 gcaactacta tgttttggtt cagctaaacc ccgaaacgtt tgaaaaacgg caggttcaac
     7921 ttggggtaag cgatgggctt agggtagaaa ttaggtctgg ccttctttcc actgaacgtg
     7981 ttgtaacccg cggggcggtt ttggtaaagc tggcacaggt atcaggaaaa cttgatgccc
     8041 acgcgggcca tgttcattaa gctgatgcgc tatgctggat aggattatta agttctcgct
     8101 gagcaataag tacaccatac ttattgcttt cttcgtgctg gcggtagtag gaattcgcac
     8161 cgcccgcaac atggatgtgg atgtttttcc cgatcttaca gcaccaaccg tagtggtgat
     8221 gactgatgcc catggcatgg cctcggagga ggtggaacgg ctggtaactt ttcccatcga
     8281 aacggcaatt aatggggcaa cggatgttcg ccgcgttcgc tcggtttcct cccagggatt
     8341 ctcgtttgtt tgggttgaat ttgactgggg cagcgatatt ttcaaagctc gccaaattgt
     8401 aagcgaaaag cttatcactg ttagttccca gctaccaata ggcgttggtc aaccggtgct
     8461 tgccccacag tcgagcgtga tgggcgaaat tttctttgtt ggtttaacag ccgatagcac
     8521 aagcatgatg gatttacgaa ccattgccga atgggaggtt aaacctatta ttcttgctac
     8581 taatggagtg tcgcaggtaa ccattattgg tggagatttt aagcagtacc aagtgctagc
     8641 cgatcctgtt aaaatgatga gctacggtgt tagcatggac gagcttgcta cagtttgccg
     8701 aaacattagc ggtaactcta ccggcagtaa tatccgtcag tttggcaacg agtatgcggt
     8761 gagaggaatg gctcgtgcct ccgacttgga gacgttggga aactccttta tcaagtttct
     8821 gaatgggcat cccgttaggg taagtgatgt tgctgaggtt gttattggaa gtgctgccaa
     8881 aatgggttat gcctcggaga atacaaaacc cgcagtcatc atttccatat ccaagcagcc
     8941 caatgccaat acccttaagg taactgaaaa cattgagaaa aatctcgata acatttacaa
     9001 aactcttcct aaggatgtta ggcttgatac cggaattttc cgtcaggccg attttattca
     9061 gacctctgtt ggcaatgttc agaacgccct tatggaaggt gctatttttg tggtgattgt
     9121 gctgttcctt ttcctgggca gcttccgcac aactattatt tcacttctgg ccatacccct
     9181 ttcgctgctt ggtacaattg tcgcacttaa ggcttttggc cttaccatca acaccatgag
     9241 tctgggtggt atggctattg ccattggttc gctggttgat gatgccataa ttgatgttga
     9301 aaacgtattt aaacgcctgc gccaaaacaa tctcaagccg gttgatgaaa gacaatcatc
     9361 ctttaccgtt gtttttgagg cttccaggga gatacgagct tcaattctaa acgctactct
     9421 tatcattatc gttgcctttc tgccattgtt tttcctgtcg ggtatggaag ggcgaatgct
     9481 taaacctctt ggcatttcat tcattgtttc gcttaccgtt tccctggtgg ttgccatgac
     9541 acttacccct cttttatcaa agatgctgct aacaaacgag agttacctgg ccaaaaacga
     9601 acgggagaag tggctttcac gtaatctgca aacctacttt aaggcatcgc tcgaatgggt
     9661 tcttcgccat aaaaaagttg tgttgggagc tgtgctgtcg cttgttattc tctccatagt
     9721 tgcttttgcc tcaatgggcc gaagtttcct accggagttt aacgaggggt cgctaaccct
     9781 gtcggtggta accaaacccg gaacatcgct tgacgaaacc aacaagctag gcaacctggt
     9841 tgagcagcag ctgcttgcca ttcccgaaat ctacagtacg gctcgcagaa cgggtagggg
     9901 tgaactcgac gagcactccc aaacggttaa cagctcggag attgatgtaa acttcaaact
     9961 gtcagagcgc tcccgcgatg agtttgttgc taacgttcgc caaacccttg ccggcattcc
    10021 gggtattgca gccacagttg gtcagccttt gggccacaga atagaccaca tgctctcggg
    10081 aactcgtgcc aacattgcca ttaaactttt cggcaccgac cttaaccaaa tgtttgccat
    10141 tggcaaccag attaaaggtg agatttccgg gatagaggga ctggttgatg tgaatgttga
    10201 tcagcaggtt gacattccac agattcagtt gaaagccaac cgggatatgc ttgccgctta
    10261 cggcattcct gttggcagct ttaacgagtt tatagatgtt gcctttggtg gcgaaaagct
    10321 ggcagatatt tacgaaggcc agcgacgttt cgatttggtg ttgaagttga atcaccgcta
    10381 cacggaaagt atcgagggta ttggcgttgc gctaattgat acctacaatg gaggtaaagt
    10441 gcctttatcg gaggtggctc aaattcagtc agtttcgggc ccaagttcca ttagtcgtga
    10501 aaatgctcag cgcaaggtgg tggtgtcggc caacgtggca ggacgagata tgagaagtgt
    10561 ggtgaacgat attcaagatc gggtgaatag ccacatcacc ttgcccgagg gatacaggat
    10621 tgagtatggc ggtcaatttg aaagcgaagc caatgcttcg cggacactaa tgataacctc
    10681 catactggcc attcttgtta tttttctgtt gctttttcag gagtttcgaa actttaagtt
    10741 ggctgccata atcctcctca acctcccact ggcactcatt ggaggaattt tctcaatcta
    10801 tataacaggt ggaattttaa gcatacccgc cattattggg tttataacac tttttggcat
    10861 tgccacccgt aatggtattc tgctggtatc caaattccag cagctgcaag cccaaggctt
    10921 accaatccac gataccatat taaaaggttc aactgacagg ttgaatgcta tactcatgac
    10981 cgccctgtcg gcagcattgg ctttaattcc tcttgccatg agggggcatc tccctggtaa
    11041 cgaaattcaa agcccaatgg ccaaggttat tctaggagga ttaatcacct ccaccatctt
    11101 aaatatctac attgtgccta ttgcatacag cttgctggcc aaacggggtt tgactaaaac
    11161 tgataagcaa tgagaagaat aagcctgata atttccaacc ttttgttgtt tgttgcaata
    11221 gctaacgcac aggtatccat cgagaagatt ttgggtgaag tagagcgaaa taataccacc
    11281 cttgttgctc tcaggcagaa gatggaggcc gaaaaaattg gcagcagaac gggactttac
    11341 cctcaaaacc ccagctttga gtattcctac cagtggggca atcctgctga tataggcagt
    11401 aaaacggaca ttagcctgat tcaatcgttc gatttcccta cagcatacct ttaccggtca
    11461 aaaattgcta aagctgaaat agaattgctt gcaacagagt accttcgtca gagtaaacaa
    11521 cttctgctgg acgttaggct ggtatgtata gagttggttt accaaaatgc cctttcatca
    11581 gtgctgggga agcgtgtcag caatgctaag ctaatagccg atgcctattc aaaaaagttg
    11641 gatgttggag aagccagcgc acttgagaac aacaaggctc gtcttaccct gcttaatgcc
    11701 acacaagagt tgtttagcgt taaacttaag caaaacgagc tacttgctaa actagctagc
    11761 ctaaatggtg gcataccgct tgagttaagc gatagcgttt tcaacatgcc cgcaatacct
    11821 gcaagttttg atgagtggta tgctgccacc gagcagtcta atccggatct taaggcaatt
    11881 gaacgggaga ttgcggtggc acaaaggaat atccagctaa gcagggcgtt atgtttacct
    11941 cagattaaag cgggatacgc tagtgaaaag gttgccggtg agcattttca gggtattgct
    12001 gcaggaattt caataccctt atgggaaaat tccaaaaggg taaagtatgc aaaggcacgt
    12061 gcccagtacc ttaggagtgt ggaagccgac cgtaaggttg aaatttttag taggcagcaa
    12121 gcgcaataca atactgctct ggagttgcaa aaaaacatag cagaatacag gaatagcatg
    12181 ggcacaatta atagcagtag gatgctggac gaagcgcttc aaaagggtga aatttcgctt
    12241 gttgattacc tgctggagga atcgctttac tatagtagcg ctgaaacatt gcttcaaatg
    12301 gaaagggatt tttgcagagt tgttgctgag ctataccagt accaatgatc caaaaatttg
    12361 actttcgaac cgtagcttta aatttttgct attttcaaca ctaaatggta aaatccctca
    12421 aatttagcag ctgtagcaat tttcatgtat tggaaccttg aacctcagat tctcaggaaa
    12481 gttgctcatg ttcataaccg ggggaacaag gggagtatgc ccatgtggaa ttattctaac
    12541 ttgccagcaa tttctgtcta aggcaattgc agctttgttt gctgttgata ttaaaaaaag
    12601 ccgtggtaag ctaccacggc tttttcaact ttttatgact ggatagtttc ctactactgc
    12661 ttaataaagc gtttggtaga aatctcttta ccttcgccta ccgacaccat gtaaatacct
    12721 tttgccaggg tacttacgtc aattcggttt tcgcttgcgt tgagaacctg cgatttaacc
    12781 atcgacccgc gtacatcgaa gatacgaact gttacctcgc tactagcacc ggttagactg
    12841 atgttgatgt aatcgcttgc cgggttgggg taaaggttga aaatttttac tccatcgttt
    12901 tctgtcaggt ctgaaattgg agtatcgaat gcgggtgaac ctgagccgct ggcaacaatg
    12961 gaaacgttgt caacatacca gtagtcaatg ttgtaaaggt tgccatcaac tacaaaagct
    13021 atgtaggtgg tttcggagtt gaggttgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnntgg aaacactcca gcgctcggta atgctggtac cggtgttctg ggtagtccat
    13201 ccggttggca gtgatgaggt gttgaaggtt tgggtaacag gaagaggaag tgatgttgat
    13261 ccgctcagcg tggtgaagct ctgctgtgcg ccgtaggcag ttccctgggc attgatggca
    13321 taagctctca cgtagtaggt agtgctggcc gaaagtcctg tcatgttagc cgtaaacgag
    13381 cctgttcccg tgccgctggt aaccttgctg ctggaagtag ttggattctg ggaggtgcta
    13441 taacaaattc cgcgttcggt aacggtggca cctccatcgc tggttacatt tccaccgcac
    13501 gatgcggagc tggaggtgat ggatgttacc gagttggttg taaccgtagg aatggttgcc
    13561 ccactacctg cagtaatgga aacgttgtca acgtaccagt agtcaatctg gtaaaggttg
    13621 ccatcaacta caaaagctat gtaggtggtt tcggagttga ggttggaggt aatgttaacg
    13681 gttacggttg ccgggccaac gttggagctg ccggtggcaa ccgaccatgt agtgttggtc
    13741 caggtggact tgttgttgga ggtctgtacc cttagggttg cacccgagcc gtaagcatcg
    13801 agcatatgct tgaacgaaag ggtaacctgg ctaaccccca cggtgttgat ggccggggtg
    13861 atgagcctgg tggtgccggg gttgacgttc tggtaggtgc acttcatctc gtaggccgag
    13921 ccaccagcct tgtaggtgtt ggaaacactc cagcgctcgg taatgctggt accggtgttc
    13981 tgggtagtcc atcctgttgg tagtgtggat gagttaaagt tttctgtaac gggaagtgtg
    14041 attgaggttg gtgtgctcga agtggtgaag ttcagggtgc ttccataggt tgttccgctg
    14101 gcattcaccg ctttaaccct aaagtagtag gtagtgttgg ccgataaacc tgtgaggttt
    14161 ccggtaacgt tagttgtagt tgttccagtt acgctacttg gagttgcgct aaaactgctg
    14221 cccagacttg tagtggttcc ccattcaaag gttaccgttg tagtggcgtt gttggcattc
    14281 acctgtccgt tcagggttgc gctgctggtt gaaattgccg aggctgccag cgtggttgct
    14341 gtgggagccg ttccgccacc agaaccgccc atgaagtcaa aggttacggt tttggttgaa
    14401 gtgttttcgg caatgttggt catgggtttg gcagtgttag cgccggccca cgatttcatg
    14461 ttgggagtag tggcatcggt gaatgatgtt ttactgctag agccaggcca tgggcatccg
    14521 gtgctgctaa tgctaccgta tgagctcgga gtgctgccgg ggttggtggt tgcactggca
    14581 caaacgggat acattttttg tggataggtt gcgttgatgt tgttgctgct gcttgccgtg
    14641 gcaatatcct tgtgaacatg gtaaattaaa agaccatgac cgggaatgtt ggcatcaaaa
    14701 ccaacctgct ggcggttctc cataatccag tactcgttgg tagtggtgga gtttatctgg
    14761 taaaagctct tgttagcctc ggcattggta accgatatgg tggctgcaga ggagagaacg
    14821 gtagcagtag cccagccgta aaccttaacc ttggtgtagg cgttatggtg ggcgggcgtt
    14881 accccgttgt tgttccacga tccgctggcc attaaatccc agtagcctgt tccttcatac
    14941 tggccaccgg tgctgtagtc ggtatcatag taatcgggag cacccagcac atgaccaaac
    15001 tcatggcaaa ttacacctat tgcagtgatt gttgttcccg aagttcccct aagctcggag
    15061 gagcaggagt acttgctgat ggttttgcca tcctttgtaa ccggagttat gctccaggca
    15121 tgagcccaaa tgcaatctgc gctggcacct gcttcctcgc catacccggc gtagataacg
    15181 taaacgccat ccactgtgcc atcgccatca ttgtcgtagt tggcatagtt ggccgaggca
    15241 tcggcggcat tcacggcttc ggttaccaat gctcttgggt taacatcata accagaggca
    15301 tcgtttgcac catagtacga catgttctgg ctggaggtgt aggggccaaa tacatcaacg
    15361 gtaaggttaa actggctcca tgaattttcc atgtagaagt ccttgacgct cccagtagca
    15421 ccgcctgtag tgtagccaac ctggttgaac aggttgttga aatccgattg tgttttggtg
    15481 aaggctttat ccttgaatcc catcaatata caaatcaact ttctgttgcc tgttgttggg
    15541 aaggccttca agctctcagc cgattttatt tcccaaatac ttttcatcag cgaaagctgg
    15601 cttgggctat agtacaaccc cttgcttacc tttcctaaaa attcacgttc cgcttttgtc
    15661 ctttcagcag ggttatttgc ctcaacgccc gatagcgtta tatcgccctt gccatcctga
    15721 aaagcatact cgtaaactcc cttgctgttt agcataaggg tgtagccatc ctcgcttagc
    15781 acccacttaa ctttttcatc accctttaaa tagatgttga tgatctttcc gttggcctgc
    15841 tcgtacttta tcaggccagg gtatgccggc acagcaacgg cttggttggt taccattgca
    15901 gctgccatta agcatacacc caagaaactt agtaaagttt tcttcatagc ttaatagaat
    15961 taattttagg ttggttaaaa aaaaggttta cttaacggga cgtaatttaa gttgctgaat
    16021 aatgcaaaat tttattttgc gacataaagc agctttctaa gtacaaacta catgttcctt
    16081 tgcacaaacg acaaaatggc ttaatatcag cattataagt cgttatgatt ctataacttt
    16141 ttaataataa cggatttgtg ggataagtta cctgctaatc tttcttaggg ataggggcga
    16201 gcaattttgt tgtaagctag ggttgttgtt ggctataaga tgatttcttt atctacctgc
    16261 attataggta gtctccatag tggaaaatgg gctcactaat gctgattttc ttttacagct
    16321 atttttcccg tcattttaaa tatacgaggt gacgagttag gtcagggtaa gaaaattctt
    16381 ttgtctagta gatttcccgt ttcgaaaaga agaatcccca ttcatgctcg gcattctcgt
    16441 agctgtcgga ggcatgtacg gcattttgag tggtattcac accaaatcgt ctgcgaattg
    16501 ttccctcgtc ggcttcctcc gggttggttt ttcccaccag ctttcgaagc gcttcaacgg
    16561 cctcaggttt ttctaaaacc attgccatca cagggcctga actcatgtag gttgtaagct
    16621 tgtcaaaaaa atccttaccc cggtggatgt cgtagaattg ctcggcctta gccttggtta
    16681 gcatggtaat ttttatgccc cttactttaa agccggcttt aagaatttca caaagtattt
    16741 cgcttacgca gtcgttctta aaagcatcgg gtttgatcat gcaaaatgtg aatcgggtgt
    16801 tcatacctta agatttttgt taacctgttt aacatgtctg gcattaatac aacagcagct
    16861 ctagcgggca aatgcttcca ttttgaaatt catgtttaaa atacttccgg tgaagcggca
    16921 cccctcttta ccggaatttt ttaaaaatag gcaaaacttt cttgtcggca aaatctatct
    16981 ttgtaaattt catcataata aaatttgatt ttaaaggtat ggtgaaacac ccatgttggt
    17041 tattttacaa tggagcttgt ggctccaaaa acatgggtgc atcatagtaa aataaaagct
    17101 cgatgaagtt gaatcttgat gaggcatggg caggggcatt aagggagttg ctggttccta
    17161 ccaaccgaat agtgctaaca acccatcata accctgatgg tgatgctgtt ggctctgtgc
    17221 ttgggctatg gcatatactt gagcaggttg gggttcatgc cactgccgtt acccctaacg
    17281 atttccccga ttttctttca tggatgccgg gaagcgatac gattctgcga ttttccagca
    17341 gtaggcaaaa ggtaagggag gtgatggctg gtgccgatat tataatttgt cttgattaca
    17401 atgggtttag ccggacagaa gatatggaat ccttgttttc ggcctcttca gccaaaaaaa
    17461 ttcttattga ccaccatccc caaccggaaa ataccttcga tttatccatt tcggttgtgg
    17521 aagtgagctc cacctccgag ctggttttcg agctggctac tgaaatttac gggctcaaag
    17581 cacttaacgc cgatgcagct acctgcctgt atgcaggcat aatgaccgat accggatcgt
    17641 ttagctacgc ctgttctcgg ggtagaaccc tcgaaattgc cggaatgctg gttgacagtg
    17701 gagccgttgt tgagcgcatt caatcgttgg tttacaataa cttttcggcc gatagaatga
    17761 agctgctggg ttactgcctg gcagataaaa tgatggtttt ccctgagttt catgctgcgt
    17821 atatctggtt gaccagggag gaactcaagc ggtttaacta tcaaatgggc gataccgagg
    17881 ggttagtcaa ctaccccctt tccatcaagg ggataatttt ttcggtgata ttcgttgaga
    17941 acgataacca tattaaggtt tcgcttcggt cgaggggtaa tttttctgtt aacgagtttg
    18001 cccgtaagta ctacaacgga ggcggccatc ttaatgctgc cggagggaaa tcatttcagc
    18061 ctattgacgc tacattggct gatttcgagc agcttctccg ccagcattta caggagttga
    18121 atgctgcagc aatataaata taggtgaaga agacgttgag atagattgct gatgaaaata
    18181 ctaaccatca tccctttagc cgttatcctg gtatcgtgtg gaggaaatga gcgccagccc
    18241 gacaggcagc ccaatgtggc tgaaactagg gagatgcttc agcgtatcaa caaggtgttg
    18301 gttcagcagg atagggagca aatactggac tacattaagc ggaacaactt ggtggagatg
    18361 aaggaaagcc ctacaggttt attttactat atttcaggga catctaatgg ccccaaggtt
    18421 agaaagggcg atgttgttga gtatagctac agggtttcgc tgctcgatgg aaccgtttgc
    18481 taccaatcca atgcagaagc cccagccacc tttgaggttg gccatggagg tgttgtttca
    18541 ggtttagaag aaggcattct gctgatgagg cagggacaaa gcgctaaatt tatcatgccc
    18601 ccccatttgg cctacggcct gattggcgat agcaaacaag tacccgctag gtcaatagtt
    18661 atttacgatg ttgaattgtt ggctgtttat cgttgatttt tgatattttg gctaacttta
    18721 cacgcattaa ttccttagct gcatgacaaa aagttcaggt attctgttag catttctggt
    18781 tgtggctacg gttggctgct ccaaccggtc gaaacttttt cctggatttt ctgtaacccc
    18841 caccggaatt cactacaagc ttatttcgct tggcaacgga acttctacgg caaagccctc
    18901 taattacatt accgctagta tttcctaccg tactgctaag gactccgttt tctttcaagg
    18961 aacccggcag ttccagcttg cccagtcaag ctacgaaggt tctattgacg agtgcttcat
    19021 gatgctggct gagggcgata gcggtgcttt ttacataaag gcactgccgt tttttactca
    19081 aaccctcgaa accaagctgc ccacatttat tggtgttaac gactacatgc gggttgacat
    19141 tcgtttgcac agcattaaaa cccaggagga gttcaaaaag gatatggagg cctttttatc
    19201 gtggattgaa gattttggcg attacgaaaa ggttatcata aggcagtata ttgatggaca
    19261 gaagctatca gttgagccta caccttcagg cctgtacatt attccccaga tacgaacgga
    19321 aagagctccg gttgaaaaag gggacaccat caccattcac tacgaaggtc gcttttttga
    19381 tgggcgaata ttcgactcca cccgcaagcg taacgaaccc tttcagcttg tttacggtga
    19441 caagtggcag gtaattccag gtattgacga agcgctggga accatgcgcg agggcgagaa
    19501 atcaatgcta attgtcccat cgcatcttgc ttttggtcag cagggaaaca ccagcggaat
    19561 tattccgcca tttacctccc tggtttttga gctggaggtg gttgaggtga aaaaaggtgt
    19621 tcagctatga ttcaaaaaaa tttctttaac gctattctac ttttgtctcc tctattgatg
    19681 ggtgcctgcg ataactcgct taaggaggat aagcgcaacg atgaggaaaa gattattgag
    19741 ggtcttataa attctaaaaa gtggacctac accaagtccg agggtgttta ccatgttgta
    19801 gttcagccat cctacggcta cgaggttaac tacggcgata cggttacctt ctggtataag
    19861 ggctacacca tccagtcccc cgtgctggtt ttcgacacca acatcaagtc ggaggctgtt
    19921 ttggcaaaac tcgacaccaa tacccgtacc cttgaacctg ttagagtcat tgcagggaaa
    19981 accaaactta ttgaggggct aaaacgaggc ctgctgctat gcagacaagg gcaaaaatca
    20041 accatatttt ttacatccga tatcggattt ggtgacaatc aggttggccc gctgccaccg
    20101 tggacacccc tggcctacga tattgaggtg gttgccctta acggtagggg aatagaatcg
    20161 gagcaaaaca ttattaaatc aatggatctg acgggatata ccctccattc tagcggcttg
    20221 tactataggt accttctcga ttcttcaaca gtaagacctc tgtcgaacag cgtggtttac
    20281 ggttggtaca gcatctccct taccgatgga actgttgtaa cccaaacacc cggggcgaat
    20341 gagaaagttg accttactgg ccaaatgccc gaagcgctac ggctaggttt cctgctggcg
    20401 tcgctggggg gcaaaatcac aattttagct ccgtctccat taggatatgg taaaagcgga
    20461 agtagcaagg tgatgcccta tgttcctatt gttgttgata ttcggctcga tagcattaaa
    20521 taagcgttgg cataaaccct gaattatgat tttttagcat tgttaaatga catgatgttt
    20581 tattttgggc aattttttaa agggtaattt gtttattttg cgtttttatt agtagtagat
    20641 attctattga actgtaatca ttaacctttg aatcgtgcaa ttttctattc ctgaaggggc
    20701 ggaataaata cgtgtgacgt gaaccgtgaa gatgttaaaa gttaaaagtt agagggaaga
    20761 agatgaatac ttttaatccg ccggtaggcg gacaagttcc gccggcaggt ggacaagttt
    20821 tgaaccctga gccagtcgaa gggtgaatcc
//
LOCUS       DAOPHX010000032        20058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_2899_length_20058_cov_6.209319, whole genome shotgun sequence.
ACCESSION   DAOPHX010000032 DAOPHX010000000
VERSION     DAOPHX010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 20058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20058
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2899_length_20058_cov_6.209319"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..1078)
                     /locus_tag="PK502_03885"
     CDS             complement(242..1078)
                     /locus_tag="PK502_03885"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPG99990.1"
                     /translation="MITVRRVIPAQQVTDGAGVKLNRVFGYDTRMETDPFLLLDYFGS
                     ENPTDYAAGFPWHPHRGIETITYMLQGKVEHSDSLGNRGEVNPGGVQWMTAGSGIIHQ
                     EMPKPISGLMYGFQLWLNLPRSFKMVPPTYHDLKSSDIPEVETSSSRVRVIAGRFRKA
                     VGLSTPYNSSIDVLDVRLWPDALFDEALNANSSYFLFVYDGQISFADYSKPIGSLNGV
                     ILDKGSILQLKAESNGANFLLFGGQPINEPIAWRGPIVMNTNDELSVAFEEINQGTFI
                     KE"
     gene            1311..4376
                     /locus_tag="PK502_03890"
     CDS             1311..4376
                     /locus_tag="PK502_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG99991.1"
                     /translation="MTKLRAFILSLTLAAAFSLAHGQETSISTREEALFAKAFELYQK
                     GLYASSQKTFQKFLSEYSSDVVAQLRTDAEFYSAICAIELNNNDAEYLLGRFIQNHPE
                     SQYIDLAYFVMGKRQYQQKNFTSSIYWLSMIDGEGLDREKRYEMQFMLGYCYFMTNDF
                     ELANKWLYQVKDMDSKFSGPATYYYSHIAYLQKKYATALKGFEKLQNDETFAPIVPFY
                     ISQIYYLQGDYQKVVAYAPPLVADNATRRTPEIARIVGDSYFKLRQYDSALVYLNKYV
                     EKTPQLSRDDYYLVGFTYYKTGNFSEAAKHLERVSTVDDSLSQNANYHLADCYLKLND
                     KPKARQAFGMAAKLDFDSFIKEDALFNFAKLTYEQLYAPFNEAIEAFRQYITLYPNTP
                     RTDEAYNYLTLAYLSTRNYKEAVDALEKIKVQDVTIKTAYQKATFYRGLELFQNMQFD
                     DAITMFAKSLENSAYNPTIAALATYWQAEAYYRLEQFEKAAEGYNRFILTSGAFSLPE
                     YLMAHYNLGYSNFKLKRYDEAVVWFRKFTNLSNDEKNVFVGDAYNRIGDSFFIQRRYW
                     AAIDYYDKAMAIGTIDADYALFQRGFALGLVERPEKKIESLQKLLTLYPSSSYADDAI
                     FEIAQSQLVLTQTGKAMENYKLIEKNYAGSSYYVRALVQLGLLYYNSENPDSAMMYFK
                     RVVEGFPNSPEAKNALMGIRNIYVDRGDANTYFNYASSLGSLANMSLAEKDSLTYISA
                     EKIYITGDCEKSNAAFSSYLRDFPQGNFSINALFYQGECYRKQGMRDRAIEAFDDVAT
                     RQKNDFTGQSLLRLGELYFEKGSYDKSYEAYAKLESVAEYKSMLQDARIGMLRCAFEQ
                     KQYADIPAIASSILSAEKLPDELERETQFKLAKSLYELGRADEALVQYSKVATNLKTA
                     EGAEAKYMKTSIYFQKKDYKKTEAEVFDFAEKNTPHQYWLAKSFILLAEVYANQDDFF
                     QAKATLNSVIDGYSSTTDGIIDEATAKLNQLVKTEKDKQSVKVDEGVTF"
     gene            4405..6204
                     /locus_tag="PK502_03895"
     CDS             4405..6204
                     /locus_tag="PK502_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPG99992.1"
                     /translation="MQSRKYTNKHTPHVEVCRLAAPYHLAKKPTILNTHKHIKQHFAP
                     NILLAGTLAFIFTTNLLVGNKAVAQETSLKKEVMVVRPYEPSISDAFKINQLPKIEDT
                     IRVTPNFTYSIVQRPAVTAFTPSPISAARMMAEPLKDLSKGYIKLGMGNAVLPVAEVY
                     FMSKRNPDFMYGAWVQYRSKIGKVKLENSKKVDASSSTADIQIFGKRMFANKILQGDL
                     GYSNNARTYYGYNILDSLTSPTSDKQRVNRVNANLNFNTSRKDSAHFNYNALASFEHL
                     GDKFDMQENRITGLGGFNKYLKQEQLGVDISFTHYSRSSQFDMQKNSILNISPWIHFF
                     GKQWRAVAGLYMVYDANSGINNTFIYPRAMLSYDVVSHYVIPYIEFGGYLEENSYSKL
                     IRENPWITPGISAWNTTHKLVMTGGVKGKFSAKVSYNVQASYSIVDSLYFYTNISINP
                     ANPLYNRFNMDFDNVELTKILGELTIAPSERFSVLIHTEYFSYKMNELAHPWHKPDYN
                     TYAELRYNLRDKIYTSIQLFAVGKRWVLSPDGVSPVRLKGYVDANLGFDYNFNNRLYA
                     FLNLNNITSSRYQEWYLYPVQGFNLQAGLSYKF"
     gene            6560..8995
                     /locus_tag="PK502_03900"
     CDS             6560..8995
                     /locus_tag="PK502_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPG99993.1"
                     /translation="MLSMPRLAAQPAGFKEIFTDAEYYLLMDDYQEALPLYQKLYKLD
                     STNSNIHYRLGQCYLNISGKKQMAIPFLEMAAQSISPKYVEGSYRETKAPLNALFYLG
                     QAYMINMELSKALNAFTQFRENLNVGDIYNLDYVNQQIKACQTAASFLEKPLNIKKER
                     FVLLPGRNVSISYPVFSGDRQSLVFTVKEKFYTALYHSVKTNGEWGTPSNITLDLGVE
                     GEVYSTSLNHDGTKLLIFINDMGSGNIYISTLEGGKWQLAKKFQGDINSRDWETFASY
                     SADDKYILFTSNRKGGVGGLDIYISELLPGDKVSPPSNLGEIINTPYNEESPILSPDG
                     NTLYFASQGHNSMGGYDIFYSKRIPNGQWSNPVNIGYPINTPDDEYFYFPFDSSTAVM
                     PLASNGESPYNELYQISINPAPPVRYVGLDGSIYLTDNSDIQSADVKIIITSDSILNI
                     RDTIVPSKIGKFTCTLTPGTYRVEAASNFYSMEPVHLYIPRNFNQEVYPLELKLQPKM
                     VAEGAVIKLQNILFDFDSYALTREAKFELEKVFNLMAENPSLYIEVTGHTDSKGSPIY
                     NLKLSAKRASAVVDYLVAKGIDERRFIVRGLGSLENLATNTNPDGSDNPAGRQFNRRA
                     SIRIFNPDKKISIEYVDVPEHLKPINSRYTILLAQPGVKPTEKQISEVEKALDMNTQV
                     YAVGSYSFICIGSFSSKSDAIESFGKVVDLNFPQAVIINENELQRIVRSSQKQLITKR
                     TIYTILVVTCEKPLNQDSFKGFPVTEEKGEDGLYRYYFGIFDTRDSAKKNLDTVNSLG
                     FPNAIIVKLEKNR"
     gene            8995..9561
                     /locus_tag="PK502_03905"
     CDS             8995..9561
                     /locus_tag="PK502_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG99994.1"
                     /translation="MTEGVLENNMVKLRAPEPDDVDLLYVWENNMEIWKVSNTITPFS
                     RHLLKKYIETAHLDIWESKQLRLIIEAKDQNALMNVPVGLIDLFDFDPYHLRAGIGIL
                     IANQQYRRRGYATEALKLLVRYCFGILQLNQIYCNISADNPVSLQLFKKQGFVEVGVK
                     KSWLKTQSGWEDEVMLQLLNPKPRLPKN"
     gene            complement(9626..11089)
                     /locus_tag="PK502_03910"
     CDS             complement(9626..11089)
                     /locus_tag="PK502_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPG99995.1"
                     /translation="MSQTEKYFEQFRKNIVGIDTTYPTVFGQKPLIYADWIASGRLYR
                     PIEERMLNVFGPMVANTHTESSYTGKFMTEAYHFALKEIKRHVNAGSDDVIVTSGFGM
                     TSSVNKFQRILGLKNYSFVFANPKERPVVFVTHMEHHSNQTSWYETIAEVVVVEPDEN
                     LMVSPANLRRTLENYSDRPMKIGSFTACSNVTGIRTPVHELARIMHEVGGLCFVDYAA
                     SAPYEEINMHPSEPLERLDAIFFSPHKFLGGPGASGVLIFNSQLYKNRVPDNPGGGTV
                     DWTNPWGEYKYIDDIERREDGGTPGFLQAIRSALAIRLKEKMGVKNIALREEELVNLA
                     FEKLKGIPGLHILAENCCQRIGSISFYIDELHYNLVVTLLSERYGIQMRGGCVCAGTY
                     GHFLLNVTKDKSHRITEMINHGDFSQKPGWVRWSLHPTTTDAEVEYIANVLREISANG
                     TSWKNDYTYCKHTNEFCSKNPEVKSLWPDIPSLFNFD"
     gene            complement(11120..11593)
                     /locus_tag="PK502_03915"
     CDS             complement(11120..11593)
                     /locus_tag="PK502_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HPG99996.1"
                     /translation="MAVEGISRLNFPEFDFRVREVGKSLQIFDAVRKKFVPLTPEEWV
                     RQHLIRYLCDFKNVPTGLIGVEVAFLLNGVNHRADVVVYGRVGKPLVVVECKAADVEL
                     SESTVDQVVRYNSYFKAPYLVITNGIKHYTFAVSWESGNSYSLNEIPDYSVMEQS"
     gene            11859..12584
                     /locus_tag="PK502_03920"
     CDS             11859..12584
                     /locus_tag="PK502_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPG99997.1"
                     /translation="MKRLLFTLVIVMAYGISNAQLFKVFGHEVGYIYVGPRLGMNFSK
                     FSDFNGTYEKSRFGYEFGAVGEFGFTTRFSIQTELMFLSKGSRDENFGKIRMNYIGIP
                     ILAKYGFRALGLDKVYVMGGTFANVRVGGAWVYDDGTTYELGGGMKKFEWGMGLGAGA
                     QYPFKTGVAALDLRYSLGFSDIHSDDNITTHSRSFEVILTYKFDLVDLMFKLKKNKTP
                     EPEPAGNEVDSNNAKGLKIEKRN"
     gene            complement(12653..13291)
                     /gene="folE"
                     /locus_tag="PK502_03925"
     CDS             complement(12653..13291)
                     /gene="folE"
                     /locus_tag="PK502_03925"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPG99998.1"
                     /translation="MEEINQGASINDEEGYSRIERWNTETTEKLAYHYSEIIKLIGED
                     ASREGLVQTPVRVAKSIQFLTHGYNVDPHEMIRSARFKEDYRQMVLVKDIELYSLCEH
                     HMIPFYGKAHVAYIPNGYITGLSKIARVVDAYARRLQVQERLTVQIRDCIQETLNPLG
                     VAVVIEAAHMCMQMRGIQKQNSVTTTSAFTGAFLTHDNTREEFIHLIGVRLH"
     gene            complement(13305..13724)
                     /locus_tag="PK502_03930"
     CDS             complement(13305..13724)
                     /locus_tag="PK502_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPG99999.1"
                     /translation="MPVVYLTRRERFSSAHRLFNPDLSDEQNLQIFGQCSHPNWHGHN
                     YELFVTVKGEVNADLGYAVNAKDISRIVHDMVVLKLDHKNINMDVDFMRGKMASTENL
                     AVSIWNELQKPLAVVGALLHCIRIVETENNSVEYYGE"
     gene            13893..14555
                     /gene="mqnB"
                     /locus_tag="PK502_03935"
     CDS             13893..14555
                     /gene="mqnB"
                     /locus_tag="PK502_03935"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HPH00001.1"
                     /translation="MNRILLVLATQVEAMPIATKKLPIGFPTAMSLTERHHVDILVTG
                     AGAVPTAYHLARNLSTYNLIINLGIAGSYRAEIMPGSLVTLGSDAFADYGIDNNGHFE
                     PINSFDFNNDTPYRLDRLESPWLNQFNLALPVVKGITVGTTSGSTERIARLREQWNPD
                     IETMESAAVFFACLAAGKPFICLRAISNAVEPRNPKNWRIKLAVDRLADCTLDLLENK
                     IA"
     gene            14558..15397
                     /locus_tag="PK502_03940"
     CDS             14558..15397
                     /locus_tag="PK502_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HPH00002.1"
                     /translation="MQSKKPLKLNFSTCPNDTFMFCAMVNRLVETKGYQFEPYLADIE
                     ELNRLAAQQLPDITKLSYGAFPELSASYQLLDSGSALGSGVGPLVVSRRKIYPDEVAY
                     TRAALPGEHTTAHLLFNLAYPNAMNKKFYLFSEIEQAVLDNEADVGVLIHESRFTYMT
                     KGLTKVADLGEFWEKRTNLPVPLGGIAIRKDLPENLKTDIAIILQSSIKFAFANRSAT
                     MPYVRRFAQEMDEEVMEKHINLYVNNFSLSLGNSGRTAVLELLKTSGKLPDNCETKSL
                     FIY"
     gene            15522..15740
                     /locus_tag="PK502_03945"
     CDS             15522..15740
                     /locus_tag="PK502_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00003.1"
                     /translation="MESHHHTNNNTKTPIFGKTQCIVDRIQSLDQKWYVEFWLIRYYF
                     MGIEVYRYKKEKVKLNGKNNELAKNGSE"
     gene            15981..16634
                     /gene="nth"
                     /locus_tag="PK502_03950"
     CDS             15981..16634
                     /gene="nth"
                     /locus_tag="PK502_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPH00004.1"
                     /translation="MSTNERYNRFIEYFNRTMPSATTELSYSNPYELLVAVILSAQCT
                     DKRVNMVTPDFIKRFPNPETLAASSTEEVYELVKSCTYPNSKAKHLVEMAQTLLSKFD
                     GKVPEDVQLLQQLPGVGRKTANVVASVAFNIPAMPVDTHVFRVARRLGLVTNAKTPLA
                     VERQLTEKIPAQLLATAHHWLILHGRYVCTARNPKCSECGLADICKEFRSKAKSSEK"
     gene            17077..19161
                     /locus_tag="PK502_03955"
     CDS             17077..19161
                     /locus_tag="PK502_03955"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPH00005.1"
                     /translation="MRRILYFALAIGIVLIFIPQKGRFKYEFQKGKPWMHDDLYAPFD
                     FPIYKTDAELFSERAEILKNFSPFYRFDSTVAQEAIQQFRVDFHETWVKFIAENNGVQ
                     PLSLSKVNELSTFYENEAINAIQHVMDSGILNKTENLVSNDMASSKLTILAGNVARET
                     LYENVFTKRKALEYVKNKLQQATPPGRTPVGNIWAYMDLQKYIQPNLMYDDFITQKYK
                     NEQIASISLTKGMIQVGERIISKGELITSSIYQVLESLKKEYEIHLGSRNSAIVTAGN
                     ALMVTALFIILYMFILSFRPEVFRNDAKILFILMLITLMAVISIYIIKSSLFSIYIVP
                     LVIVPIYILTFFDSRLALFTHLVTIFLVGFFVPNSFEFVFINFIAGMVAIISMTNFYR
                     RGRLFVTVGMVYATYALLLVGLAAVEEGNIKDFESINLLWFAINALLLLALYQLVYLF
                     ERIFGFLSDATLIELSDTNLDLLRKLAEVAPGTFQHSLQVANLAETAIQHVGGNPLLV
                     RAGVLYHDIGKMANPLYFIENQPYDFNPHRNLDFTESASIIINHVTEGVRIGRKAGLP
                     DQLIDFIQTHHGTSKVRYFYQRHLDKFDNGAADAVKFTYPGPIPPSKETAILMMADAV
                     EAASRSMKNVTEENIKELVDTLINQQLADGQFNDANITFKDISTIKSVFKKKLLNIYH
                     ARIEYPETKANE"
     gene            complement(19250..19660)
                     /locus_tag="PK502_03960"
     CDS             complement(19250..19660)
                     /locus_tag="PK502_03960"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPH00006.1"
                     /translation="MDKLAELFELEMEVRDYECDLEGIVNNAVYLNYLEHARHKYLLS
                     WGIDFARLHTEGIDLVVTRIEIDYKRSLASGDKFKVRVSARREGHLRLVFDQEVIAIP
                     DGSTAVKAKVIGVGLKQGRPVKIEGIPGFENFSA"
     gene            complement(19731..>20058)
                     /locus_tag="PK502_03965"
     CDS             complement(19731..>20058)
                     /locus_tag="PK502_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPH00007.1"
                     /translation="SPATSLSIGQQQRLCLARGLAVKPRIILADEPTSALDPISSKII
                     ENLFRNLKENYTIILVTHVLRQAIRLADHVIFMYNGEIIEQGTPDELFNNPKSEKLKE
                     YMVEGN"
BASE COUNT         5884 a         4804 c         4244 g         5126 t
ORIGIN      
        1 tttaagcccc gaaggggcta gagagcctag cccccgggca aagccctggg ttttaaggtg
       61 gccacgggta tgttggcgat gcaagtagga gcctctctaa agaaccattg agaccttttg
      121 catcgcaaca ggaaaaaata aggtttatat cacacccctt caggaattca gggaaaaatt
      181 gcacgaagtg aaatcaatac caacaatact cctctgcccg ttaaatctca gagcaaaaaa
      241 cttactcctt aatgaatgta ccctggttta tctcctcaaa tgcaacgctg agctcatcgt
      301 tggtgttcat tactatgggg cctcgccatg ctatgggttc atttatgggt tgaccaccaa
      361 ataagagaaa gttggcacca ttactttcag ctttaagctg cagaatagat cccttgtcga
      421 gtataacacc atttaagctg ccaataggct tagagtaatc ggcgaacgag atttgcccat
      481 cgtaaacaaa caggaagtag ctggagttgg cattcagcgc ttcgtcaaaa agggcatcgg
      541 gccatagcct aacatcaaga acatcaatgc tgctgttgta gggtgtactt aatccaacgg
      601 ccttacggaa tcgaccggca attaccctaa ccctcgatga gcttgtttca acctccggta
      661 tatcggatga ttttaaatcg tggtaggtag gaggtaccat tttaaagcta cggggaagat
      721 tcagccaaag ctgaaaacca tacattaacc ccgaaatggg cttaggcatc tcctgatgga
      781 ttattccgct acctgcagtc atccactgta caccgcccgg gttaacttca cccctattgc
      841 caaggctgtc ggaatgctcg acctttccct gtagcatata ggtgatggtt tcaattccac
      901 gatgggggtg ccatggaaat ccagcagcat agtcggtagg attttcggaa ccaaagtaat
      961 ccagcaagag gaagggatca gtctccattc ttgtgtcata cccgaaaacc ctatttaact
     1021 ttactcctgc accatcggta acctgctggg cgggaataac tctccttacc gtaatcacca
     1081 cctgcttatt ttagaccgtt aagttagtaa atattttgat aatgtcaaat taaaactctt
     1141 atggcaagtt tttaacgtgc atattgtaga accaggagcg cttttactta ctatcagact
     1201 attttttaca ataacactta cacccaatgc tttgttgaca aaaaaatgaa gagccagtag
     1261 gcagtttttc catttcttac tacttttatc ggaaattaag atttgatggt atgacaaaac
     1321 taagggcgtt tatactttct ttaacgctag ctgctgcctt tagccttgct cacgggcagg
     1381 aaacaagcat aagcacccgt gaggaagctc tttttgcaaa agcctttgag ctttaccaga
     1441 aggggttgta tgccagctcg cagaaaacat tccagaagtt tctgtctgaa tattcctcgg
     1501 acgttgtggc ccagctaagg accgatgcag aattttacag cgccatttgt gccattgaac
     1561 ttaacaacaa cgatgctgag taccttcttg gccgcttcat tcaaaatcat cccgaaagcc
     1621 agtacattga tttagcctac tttgtcatgg gcaagcggca gtaccagcaa aagaacttta
     1681 ccagctcaat ctactggcta tccatgattg acggggaagg actagacagg gaaaaacgct
     1741 acgagatgca gttcatgctg ggttactgct actttatgac gaacgatttt gagctggcaa
     1801 acaagtggtt ataccaggta aaggacatgg acagcaaatt ttccgggcct gcaacctact
     1861 actactcaca cattgcctac cttcaaaaaa aatatgcaac cgcactaaag ggttttgaaa
     1921 agcttcagaa cgatgaaacg tttgctccta ttgttccctt ttacatatcc caaatctact
     1981 acttgcaggg cgattaccaa aaggtagtgg cttatgctcc tccactggta gccgataatg
     2041 ccaccagacg aacccctgaa atagcacgaa tagttggtga ttcatacttc aaacttcggc
     2101 agtacgacag cgcgctagtt tacctgaaca agtatgttga aaaaactccc cagctatcgc
     2161 gcgacgatta ctacctggta ggatttacct actataagac agggaatttt tctgaagcag
     2221 ccaagcacct tgagcgagtt tccacggttg atgactcgtt aagccaaaat gccaactacc
     2281 acctggccga ctgctacctg aagctaaacg acaagcctaa agccaggcaa gcttttggaa
     2341 tggctgcaaa gctagatttc gactctttta tcaaggagga tgcacttttt aactttgcca
     2401 agctcaccta cgagcagctt tacgcaccct tcaacgaggc aattgaagca ttcaggcagt
     2461 acatcaccct ttacccaaac actccacgta ctgacgaagc ctataactac ttaaccttgg
     2521 cctacctaag cacaagaaac tacaaggagg cggtagatgc gctcgaaaaa atcaaagtgc
     2581 aagacgtaac cattaaaacc gcataccaaa aggcaacatt ctacagagga ttggagctat
     2641 tccaaaacat gcaattcgac gatgccatta cgatgtttgc aaaatcgctc gaaaattcag
     2701 cctataaccc tacaattgca gcgctggcaa cctactggca ggccgaggct tattatagac
     2761 tagagcagtt tgaaaaagca gctgagggct acaaccgatt cattttaact tccggtgcct
     2821 tcagcttacc cgagtaccta atggcacact acaaccttgg ttattccaac ttcaaactta
     2881 aacgttacga tgaggccgtt gtgtggtttc gcaagtttac caaccttagc aacgatgaga
     2941 aaaacgtttt tgtcggcgat gcctacaaca gaatcggaga ttcatttttc attcagcgtc
     3001 gctactgggc tgccatagac tactacgata aagccatggc tattggcaca attgatgccg
     3061 attatgccct gttccaaaga ggcttcgctc ttgggctggt cgaaaggccc gaaaaaaaga
     3121 ttgaatccct tcagaagctg ctaaccttat accccagctc aagctacgct gacgatgcaa
     3181 tttttgaaat tgcacaaagc cagctggttc ttacacaaac aggcaaggct atggaaaact
     3241 ataagctgat tgagaaaaac tatgccggta gcagctacta cgtaagggca ttggttcaac
     3301 ttgggctact ctactacaat tccgaaaacc ctgatagcgc catgatgtac ttcaagcgcg
     3361 tggtggaagg attcccaaat tctcctgaag caaaaaatgc cctgatggga ataagaaata
     3421 tttacgtgga caggggcgat gcaaacacct acttcaacta cgcctccagc ttgggcagcc
     3481 tggccaacat gagcctagcc gaaaaggact cgttaaccta catttcggcc gaaaaaatct
     3541 atataacggg cgattgtgaa aaatcaaatg ccgctttttc gagctacctg cgcgatttcc
     3601 cccagggcaa cttcagcatt aacgcccttt tttaccaggg tgagtgttac cgcaagcaag
     3661 gcatgcgcga tagggcaatt gaggctttcg atgatgttgc tacccggcaa aaaaacgact
     3721 tcaccggtca gagcctactc aggttaggcg aactttattt tgaaaaggga agctacgaca
     3781 aaagctatga ggcatacgca aagcttgagt cggttgcgga gtacaaatca atgcttcagg
     3841 atgccaggat aggaatgctg cgctgtgcct ttgaacaaaa gcaatatgcc gacattccgg
     3901 caatagccag cagcatactt tcggcagaaa agctacccga tgagcttgaa cgtgaaaccc
     3961 aattcaagct ggctaaatcg ctctacgaac ttggaagagc cgacgaggcc ttagttcagt
     4021 attcaaaggt ggctaccaat cttaaaacag ccgagggggc agaggcaaag tacatgaaga
     4081 ccagcattta cttccagaaa aaggattaca agaaaaccga agcggaggtt ttcgattttg
     4141 ccgaaaagaa caccccccac cagtactggc tggccaaaag cttcatactg ctggccgaag
     4201 tgtacgccaa ccaggatgac ttctttcagg ccaaagctac ccttaatagc gttattgacg
     4261 ggtattccag cacaaccgat ggcattattg acgaggctac cgctaagctt aaccagctgg
     4321 taaagaccga gaaggataag cagtcagtaa aggttgatga gggtgttact ttttagatgt
     4381 tccattcttt taaactagcc agacatgcag agcagaaaat acaccaataa gcacacacct
     4441 catgttgagg tttgccggtt ggcagcgcca taccatttag ccaaaaaacc aacaatattg
     4501 aatacccata agcacataaa acaacatttt gcgcccaata tactgctggc aggcacactg
     4561 gcgtttatat ttaccacaaa cctgctggtt ggtaataagg ctgtagccca ggaaaccagc
     4621 cttaaaaagg aggtaatggt tgttagacca tacgagccct ccatttctga tgccttcaaa
     4681 ataaaccagc tccctaaaat agaggacact attagggtta ccccaaactt tacctactcc
     4741 atcgttcagc ggcctgccgt tacagctttt acacctagcc ctatctcagc agctcgaatg
     4801 atggccgaac cattgaagga tttatcaaag gggtacatta agctggggat gggaaacgca
     4861 gtacttccag ttgccgaggt atacttcatg agcaagcgta atccggactt tatgtatggc
     4921 gcttgggtac agtaccgctc aaagattggg aaggttaaac tagaaaacag caaaaaggtt
     4981 gatgccagct catccacggc tgatatacag atttttggga aaagaatgtt tgccaataaa
     5041 attcttcagg gagatttagg ctatagcaac aacgcaagaa cctactacgg gtacaacata
     5101 ctggatagcc tgactagccc aacatccgat aagcaaaggg ttaacagggt aaacgctaac
     5161 ctcaacttca acacatcccg gaaggattca gcccatttca actataacgc cctagctagc
     5221 tttgaacatc taggcgataa gttcgatatg caggaaaaca gaattaccgg attaggagga
     5281 ttcaataagt atttaaagca ggagcaactt ggcgttgata tctcatttac ccactactcc
     5341 cgaagcagcc agtttgacat gcagaaaaat tctattctga acatttcacc atggattcat
     5401 ttctttggga agcagtggcg ggcagtagcc gggctataca tggtttacga tgccaactcc
     5461 ggaatcaaca acaccttcat ctatccacgc gccatgctct cctatgatgt tgtaagccac
     5521 tatgttatac cctacattga atttggaggc taccttgagg aaaactccta ctccaagctt
     5581 attcgggaga atccctggat aacgcctggc attagcgcct ggaacacaac ccataagctg
     5641 gtgatgacgg gtggagtaaa ggggaagttt agcgccaagg tatcctacaa tgttcaggcc
     5701 tcctactcca tagttgacag cttatacttc tatacaaaca tcagcatcaa tccggcaaat
     5761 ccgctgtata ataggtttaa catggatttc gataacgttg agcttactaa aattttaggc
     5821 gagctcacca tcgctccatc ggaaaggttc agcgttctga ttcacaccga gtacttcagc
     5881 tacaaaatga atgagctggc acatccatgg cataaacccg actacaacac ctatgctgag
     5941 ctacgctata acctaaggga taagatatac accagcatac aactttttgc cgtaggcaaa
     6001 cgttgggtgc tctcgcccga cggggtatcg cctgttaggc ttaaagggta cgtggatgcc
     6061 aacctgggat tcgactacaa cttcaacaac aggctttatg cattcctaaa cctgaacaac
     6121 atcacctcgt cacgctacca ggaatggtat ctctatcctg ttcagggatt caaccttcag
     6181 gctgggctat cgtataaatt ctagccggaa atgttgaaag gttaagatat caggctcaac
     6241 tttgaccttt ccacagcatt tgcaccattt tttcgtggaa caaatcgtat cctttcaatt
     6301 cgtaaccccc tttaaccaaa cataggaagc tgctcgaaat tgcacctgag gcaatttact
     6361 ggctacacaa ttatttttct tcaagttata ataggccaac ccgcaggatt ttaaaaaaat
     6421 ggactttatg gttaaataat gctaaatttg tttcttgaaa ttatttaact gccgaacaat
     6481 gcactgccaa acagcaaaag agattaaatc aagcttgata acacacgggt taggttggat
     6541 ttttttaatt tcaaccttca tgctgagcat gcctcggcta gctgcccaac ccgcaggatt
     6601 taaggaaata tttaccgatg ccgaatacta ccttctgatg gacgattacc aggaagcgct
     6661 acccctttac cagaaacttt acaagctgga ttctaccaac agcaatatcc attaccggct
     6721 tggccagtgc tacctgaata tttccgggaa aaaacaaatg gccataccct tcctagaaat
     6781 ggccgctcaa agcattagcc caaagtacgt agaagggtca taccgtgaaa caaaagctcc
     6841 gttgaatgcc ctcttttacc ttgggcaggc ctacatgatc aacatggagc tttccaaagc
     6901 cctcaatgcg tttacccaat tccgggagaa cctgaacgtt ggcgatatat acaacctcga
     6961 ctatgtaaac caacagatta aggcctgcca aaccgccgcc tcttttctcg aaaaaccgct
     7021 caacataaaa aaggagcggt ttgtcctgct tccaggcaga aatgttagca ttagctatcc
     7081 ggttttctcg ggcgataggc aaagtttagt atttactgta aaggaaaaat tctacaccgc
     7141 tctataccat agcgtgaaaa ccaatggtga atggggaaca cccagcaaca tcacccttga
     7201 cttgggcgta gaaggtgagg tttactccac atcccttaac catgatggca ccaaactcct
     7261 tatttttatt aatgacatgg gttcagggaa tatctacata tctacccttg aaggtggaaa
     7321 gtggcaactt gcaaagaaat ttcagggtga tataaactct agagactggg aaacctttgc
     7381 ctcctactcg gccgatgata agtacattct ttttacttcg aaccgtaaag gtggcgttgg
     7441 agggcttgat atatacattt ccgagttact tcctggtgat aaagtttcgc ctccgtccaa
     7501 ccttggtgag atcataaata caccctacaa cgaggaatcg cccattttaa gccccgatgg
     7561 caataccctt tacttcgcat cacagggaca taacagcatg ggcggatacg atatcttcta
     7621 ctccaaacgt attcccaacg ggcaatggtc aaatcctgta aatatcggat accccataaa
     7681 cactcccgac gatgagtatt tttactttcc ttttgattcc agcaccgccg ttatgccctt
     7741 agcctccaac ggtgaatcgc catacaacga actttaccaa atctccataa atcctgcccc
     7801 accggtaagg tacgttggac tcgacggctc catctacctt accgacaact ccgacatcca
     7861 atcggctgat gttaaaatta tcattacctc cgacagtata ctgaacataa gagataccat
     7921 tgttccctcc aaaattggca agtttacatg tacactaaca ccagggacat acagagttga
     7981 agcagccagc aacttctaca gcatggagcc ggtgcacctg tatattcctc gcaactttaa
     8041 ccaggaggtt tatccgctag agctgaaact tcagcccaaa atggttgccg agggcgccgt
     8101 catcaagctg cagaatattc ttttcgactt cgacagctac gcccttaccc gcgaagctaa
     8161 gtttgaactt gaaaaggttt tcaacctgat ggccgaaaat ccatcattat acattgaggt
     8221 tacagggcat accgatagta agggctcgcc catttacaac ctaaaacttt cggccaagcg
     8281 cgccagtgcc gtagtggatt acctggttgc aaaagggatt gacgaaagac gtttcattgt
     8341 tcgcgggcta ggatcgctgg aaaatcttgc cacaaacacc aatcccgatg gaagtgataa
     8401 tccggccggg agacaattca accgtcgtgc cagcattaga attttcaacc ccgataaaaa
     8461 aatcagcatt gagtatgttg atgttcctga acatcttaaa cccataaact ctcgatatac
     8521 catactacta gcccaacccg gagttaagcc tacagaaaaa cagatttcag aggtggagaa
     8581 agccctggac atgaatactc aggtctatgc ggttggttcc tacagcttta tatgcattgg
     8641 cagcttctct tccaaatccg atgccatcga atcgttcggg aaggttgttg acctcaactt
     8701 cccacaagcg gtaattatca acgaaaatga gctgcagcga attgtcagat cctcgcaaaa
     8761 gcagctaatc accaaacgca ctatctacac catactggtg gtaacctgcg aaaagccgct
     8821 aaaccaagac tcattcaaag ggttcccggt aacggaggag aaaggcgaag acggtctgta
     8881 caggtactat tttggcatct ttgacaccag ggactctgcc aaaaagaatc tcgacaccgt
     8941 taactctctg ggatttccga atgcaattat agtgaagctc gaaaagaaca ggtgatgact
     9001 gaaggtgttt tggaaaacaa catggtaaaa ctcagggcac cggaacccga tgatgtggat
     9061 ttgctctacg tttgggaaaa caacatggaa atatggaagg tgagcaacac cattacccca
     9121 ttctcacgcc acctgctaaa aaaatacatc gaaacagcac acctcgacat ttgggagtca
     9181 aaacaacttc gactcatcat tgaggctaag gatcagaacg ccctgatgaa tgttcctgta
     9241 gggttgattg acctttttga cttcgacccc taccatctta gggcagggat aggtattctg
     9301 attgcaaacc agcagtaccg tcgcagaggc tatgctactg aggcactaaa actcctcgtt
     9361 cggtactgtt ttggcatcct ccagctaaac cagatttact gcaacatatc ggccgataac
     9421 ccggttagcc tgcagctctt caagaagcaa ggattcgttg aggttggggt aaaaaaatca
     9481 tggttaaaaa cgcaaagcgg ctgggaggat gaggtaatgc tccagctcct taaccccaaa
     9541 ccacgcctac caaaaaacta acccggaata aaagctgcca gggttcaaac cacaagctgc
     9601 acgagtaaaa aattccgagt tgcaattaat caaagttaaa aaggctcgga atatctggcc
     9661 atagagactt aacctctgga tttttgctac agaactcgtt ggtgtgctta cagtaggtgt
     9721 agtcattctt ccacgatgtg ccgtttgctg aaatttcccg aagcacattg gcaatgtact
     9781 ccacctcggc atcggtggtt gttggatgta gcgaccacct aacccagccg ggcttctgag
     9841 aaaaatcgcc atgattaatc atttcggtaa tccggtgcga tttatcttta gtaacattca
     9901 acaggaaatg gccgtatgta ccggcacaaa cacacccgcc ccgcatttga atgccgtagc
     9961 gctcgcttag cagggtaacc accaggttgt agtggagctc atcaatgtag aatgaaatag
    10021 agccaattct ttggcagcag ttttcggcca aaatatgaag cccggggata cccttcagct
    10081 tttcgaaggc aagattcacg agctcctcct cgcgaagggc gatattttta acccccattt
    10141 tctccttaag cctgatggcc agtgcagagc gaatggcctg aaggaaaccg ggcgttccac
    10201 catcctcccg cctttcgata tcgtcaatat acttgtactc accccagggg ttcgtccagt
    10261 ccaccgttcc gcctccaggg ttatcgggaa ccctgttttt ataaagttgc gagttgaaaa
    10321 ttagcacgcc cgaagccccg ggcccaccaa gaaacttgtg gggtgaaaag aatatggcat
    10381 ccagcctctc aagtggttcg gaggggtgca tgttaatttc ttcataggga gcagatgcgg
    10441 cataatcaac aaaacaaagg cctccgacct cgtgcatgat acgcgccaac tcgtgaacag
    10501 gagttcggat accggttaca ttgctgcaag ccgtaaagga accaatcttc attggcctgt
    10561 cgctgtaatt ctcaagtgtt cttcttaaat tagctggtga aaccatgagg ttctcgtcgg
    10621 gctccactac caccacctcg gcaatagttt cgtaccatga ggtttggttg gagtggtgct
    10681 ccatgtgggt aacaaacacc accggccgct ctttagggtt ggcaaagaca aagctgtagt
    10741 tttttaaccc caggatgcgc tggaatttat ttactgagga tgtcattccg aaacccgaag
    10801 ttacaattac atcatccgaa ccagcattta cgtgccgttt tatctccttt agggcaaagt
    10861 ggtaggcctc ggtcataaat ttcccagtgt agctgctctc ggtatgggtg ttggccacca
    10921 tggggccaaa aacattcagc atcctctcct ctattggccg gtatagcctg ccactggcaa
    10981 tccagtcggc ataaattaat ggcttctgcc caaaaacagt ggggtaggtt gtatcaatcc
    11041 ctacaatgtt cttcctgaac tgctcaaagt acttctcggt ttggctcatt tcatagtaat
    11101 attttgagaa aaattatctt caactttgct ccatcacgct gtaatcagga atttcattta
    11161 gactgtagga gttccccgat tcccagctta cagcaaaggt gtagtgttta attccgtttg
    11221 taatcaccag gtagggagcc ttaaagtatg agttgtagcg aacaacctgg tcaacagtgc
    11281 tctcgctgag ttcaacatca gcagccttac actctacaac cactaacggc ttcccgactc
    11341 taccatagac caccacatcg gctctatggt ttaccccatt cagcaaaaaa gcaacctcca
    11401 cccctattaa tcccgtggga acattcttaa aatcgcaaag gtagcgaata aggtgttgcc
    11461 tcacccactc ctcaggggtt agaggtacga attttttcct tacagcatca aaaatctgta
    11521 agcttttacc aacctccctt actcggaagt caaattctgg gaaattgagc ctgcttatac
    11581 cttcaacagc catagggcaa aagtactaaa ttccggttgg ggtgcaaatg ccaaccaatc
    11641 aacctataga gtagaaattt gagataaaga aaatactgtt cattttttca gatactcaag
    11701 gcttctaaat atgaacgggt taaaccataa gtgaataaaa tatcagcaaa tatttgctaa
    11761 ccaaccaaaa atgaaatttt tcatttcaaa cccgctatcg gtttcaaaaa atatttgtta
    11821 atttgtgtta ctagataacc taaaaaatta acctaataat gaagaggcta ctattcaccc
    11881 tggtcatagt aatggcttat ggcatctcga atgcacagct gttcaaggtt tttggacacg
    11941 aggtagggta tatttacgtt ggacccaggc ttggcatgaa cttttccaag ttttccgact
    12001 ttaacggcac ttatgaaaaa agccggtttg gatatgagtt tggagcagtg ggggagtttg
    12061 gtttcaccac tcgtttttcc attcaaaccg agctgatgtt cctgtcgaag ggaagcaggg
    12121 atgagaattt tggaaaaatc aggatgaact acatcggcat acccattctg gccaaatacg
    12181 gattcagggc gttggggctg gacaaggttt atgtgatggg cggaacgttt gccaacgtaa
    12241 gggttggcgg cgcttgggta tatgacgatg gcactaccta tgaactggga ggtggtatga
    12301 aaaaattcga gtggggaatg gggctgggtg caggagcgca gtacccgttt aaaactggtg
    12361 tagcagcttt ggatttaagg tactcattgg ggttctcaga tattcattcg gatgataata
    12421 ttacgactca ctccagatca ttcgaggtaa ttctcaccta caagttcgat ttggttgacc
    12481 tgatgttcaa gttgaagaag aataaaactc cggaacctga accagcaggt aacgaagtgg
    12541 atagcaacaa tgccaagggg ctgaagattg aaaaaagaaa ctaaccctta gctattacct
    12601 tatttatttt gtagaagcct gccatcaggc ttttattgtt aaaagtgata agctagtgca
    12661 gcctgacacc aatcaagtgg ataaactctt cgcgggtgtt atcgtgggtt aggaaagcac
    12721 cggtgaatgc agaagtggtg gttacggagt tctgcttctg aatgccccgc atctgcatgc
    12781 acatgtgagc agcttcaatc accacggcca ctcccaatgg gttgagcgtt tcctgaatgc
    12841 agtcgcggat ttgaacggta agccgttcct gaacctgaag ccttcgggca taggcatcta
    12901 caaccctggc aattttactt aaaccggtga tatacccatt tgggatgtag gcaacatgag
    12961 ctttcccgta aaagggtatc atgtggtgct cgcacagcga gtagagctcg atatccttca
    13021 ccagcaccat ctgccggtaa tcctccttaa acctggcgct tcgtatcatc tcatgtgggt
    13081 caacattata gccgtgcgta aggaactgaa tggacttagc cacccttaca ggagtttgaa
    13141 ccaagccctc ccgcgaggca tcctcgccaa taagcttgat tatctcactg tagtggtagg
    13201 caagtttttc ggttgtctcg gtgttccaac gctcaatccg ggagtaaccc tcctcatcgt
    13261 ttatagatgc gccctggtta atttcttcca tttccacaat aaatttattc cccgtagtac
    13321 tctacagagt tgttttctgt ttctactatc ctgatgcagt gaagtaaagc accaacaacc
    13381 gctaaaggtt tttggagctc attccagatt gaaacagcca ggttttcggt tgaggccatt
    13441 ttgcccctca taaaatcaac atccatgttg atgttcttat ggtcaagctt cagcactacc
    13501 atgtcatgca caatccggct aatatccttg gcattcaccg cataccccaa atccgcatta
    13561 acctcgccct taactgtaac aaatagctcg tagttatgcc catgccagtt gggatgcgag
    13621 cactgaccaa aaatttgaag attttgctca tcgctcaaat ccggattaaa aagacggtgg
    13681 gccgagctaa aacgctccct tcgtgttagg taaacaacag gcatataaac cactgtttag
    13741 cgaaaacaaa ctacaaaact aagctcttaa atttaaagtt atacgctttt tgtaattttg
    13801 atgagctaaa atgaaactat acgactcatg taggcagttt aacattttag gaattttatt
    13861 gttcaaaacg gtaatcaggc gaatttaaga acatgaaccg aattcttcta gttctggcaa
    13921 cccaggttga ggccatgcca atagccacaa agaagctacc cataggcttt ccaacggcta
    13981 tgagccttac ggaaagacac cacgtagata ttctggttac cggagctggt gcagtgccca
    14041 cagcatatca tctggccagg aatttatcaa cctacaacct cattataaac ttaggcatag
    14101 ctggtagcta ccgggcagaa attatgccgg gcagcttggt tactttaggt agcgacgcat
    14161 ttgccgacta tgggatagat aacaacgggc atttcgagcc aatcaatagt tttgatttta
    14221 acaatgacac tccttaccgg ctcgataggc tagaaagtcc ctggttaaac caatttaacc
    14281 tagcgcttcc tgttgtaaag ggcattactg ttggaactac tagcggttca acagaaagga
    14341 ttgccaggtt gcgagaacaa tggaatcccg atattgaaac catggagtcg gcagctgtct
    14401 tttttgcttg cctcgctgcc ggcaagcctt tcatttgcct gcgcgccatt tccaacgccg
    14461 ttgagccccg aaacccaaaa aactggagaa ttaaactggc cgttgatagg ctagccgatt
    14521 gcaccctcga cctgctggaa aataaaatag cataatcatg caaagcaaaa aacctcttaa
    14581 acttaacttt tctacctgcc ccaacgatac ctttatgttc tgcgctatgg ttaaccgctt
    14641 ggtagaaacc aaagggtatc agtttgaacc ctacctggcc gacatagagg agttgaaccg
    14701 gctagcagca caacagctac cggatatcac caagctaagc tacggggcat tcccggagct
    14761 ttcggccagc taccagctgc tggactccgg tagcgccttg ggaagcggcg ttggccctct
    14821 ggtagttagc agacgtaaaa tttaccccga tgaggtggcc tacaccagag ctgctctccc
    14881 cggcgaacat actacagccc acctgctatt caaccttgcc taccccaatg caatgaataa
    14941 aaaattctac cttttttccg aaatcgagca ggctgtgctg gacaacgagg cagatgtagg
    15001 tgtgctcatc catgagtcac gatttactta catgaccaag gggctaacta aggtagccga
    15061 tttaggtgaa ttctgggaaa aaagaaccaa cctacccgta cccctcgggg gaattgccat
    15121 tagaaaagac ttacctgaaa acctgaaaac cgacattgcc ataatattgc aaagcagtat
    15181 aaagtttgca tttgccaatc gctctgccac catgccctat gtcagacgct ttgcccagga
    15241 aatggacgaa gaagttatgg aaaaacatat taacctctac gtcaacaact tctctctatc
    15301 actgggtaac tcgggcagaa ctgccgtgct ggagctcctt aaaacatccg gaaaactgcc
    15361 ggacaactgt gaaaccaagt ctcttttcat atactgaatg atagccttgg cattgcgcaa
    15421 gctgcttttc gccaaataat ttgaattttt acagctcaaa ccctttaaac tttgatttga
    15481 aaacgatatt tttatgatta caataacttt ttaaatgctc aatggaaagt caccatcata
    15541 ccaacaataa caccaaaaca cccatttttg gaaaaactca atgcattgtt gacaggatac
    15601 aatcgctcga ccaaaaatgg tatgtagaat tctggctgat tcgttactac ttcatgggaa
    15661 ttgaggttta caggtacaaa aaggagaagg ttaagctgaa tgggaaaaac aacgagcttg
    15721 ccaagaatgg tagcgagtag aatccacgct aaaatctaat acttacctct taaatagcca
    15781 tccatatccc attttttctt ttccggaagc aatagatgga tgatcaactc aaagattatt
    15841 tataaaaagc catagtgcga ccatctcaat acaaccaacg ttaacgggat agtaaatttt
    15901 ggatatttct gagataaata acaagatttt gttgccctgc cttcagtata tttgaaaaaa
    15961 atattgccat atacctgatg atttctacaa acgaacgcta caaccgattt atagagtact
    16021 ttaaccgaac catgccgtcg gccacaacgg agctgagcta cagcaacccc tacgagttac
    16081 tggtagcagt aatcctaagc gcccagtgca ccgacaagcg tgtgaacatg gtaactcctg
    16141 attttattaa acgcttcccc aaccctgaaa cgctggcagc atcaagcacc gaggaggtgt
    16201 acgagctggt gaaaagttgc acctacccca atagcaaggc caagcatctg gtggaaatgg
    16261 ctcaaaccct gctctccaag tttgacggca aggtgcccga ggatgttcaa ctgctacagc
    16321 agctgccggg agttgggcgt aaaacagcaa acgtagtggc ctcggtagct tttaacatac
    16381 ctgccatgcc tgttgatacg catgttttca gggttgccag acgtttaggt ttggttacta
    16441 atgccaaaac accgctggca gtcgaacggc agcttactga aaaaatcccg gcccaactgc
    16501 ttgctacagc gcaccattgg cttatactac acggacgcta tgtttgtaca gcccgaaatc
    16561 caaaatgtag cgaatgtggc ttagctgaca tttgcaaaga gtttcgcagc aaggcaaaat
    16621 cttcagaaaa ataaaagcca gctatacagt agtcaaccat ctaaggttcg gagaacatca
    16681 aaaaaatatt atgcccaaat accggggatg tacccatgca aagaagtggc ttcaaatgga
    16741 accctgaact acaggtaatt tcttgcagct attctttatt aaacaaccag gtagggtaac
    16801 ttgcataaac aagcatgaat aaccgtgcgt tacctacgag ttcggcgaaa cctaaacatg
    16861 ggtatccttt ccgcttaacc cccatgccta aagggtatga aaatcataaa cctaaaagta
    16921 acgtacaaat gaaaaaagcg aaatgattca accaaacaaa ataaaaagca gctttcattt
    16981 ctgcatttct atatttattg tcatttttgc cttcacatag ctagcattaa tcaaagtatg
    17041 agcatgcccc atgttgtcaa aaaaaatttc aggctgttgc gccgcatatt atacttcgca
    17101 ttagccatcg gaattgtgct gatattcata ccccaaaaag ggaggttcaa gtacgaattt
    17161 caaaagggta aaccttggat gcacgatgat ctttatgctc cctttgattt tcccatatac
    17221 aaaacagatg cagagctctt ttcggagcga gccgagatac taaaaaattt tagccccttt
    17281 taccgcttcg actctacggt tgcacaggag gctattcaac agttcagggt tgactttcac
    17341 gaaacatggg tcaaattcat agccgaaaat aacggtgttc agcccctttc cttatcaaaa
    17401 gtcaatgagt tgtcaacttt ttatgagaat gaggctatca acgccattca gcacgtaatg
    17461 gacagcggga tactcaataa aacagaaaat ctggttagca atgacatggc aagctccaag
    17521 ctaacaattc ttgccggtaa cgtagctcgc gaaactcttt acgaaaatgt ttttaccaaa
    17581 cgtaaggctc tggagtatgt aaaaaacaag cttcaacaag ccactccccc tggcagaaca
    17641 cccgtaggca atatatgggc ttacatggat ttacaaaagt acatccagcc caacctgatg
    17701 tatgatgatt tcataaccca aaagtacaag aacgagcaaa ttgcgagcat atcgctaacc
    17761 aaggggatga tacaggtagg cgagcgcatc atatcaaagg gtgaactcat cacttccagt
    17821 atataccaag tgttggaatc gcttaaaaag gagtacgaaa tccatctggg cagcagaaat
    17881 agcgctatag ttacagcggg taatgcattg atggttaccg cattgtttat tattctctac
    17941 atgtttatcc tcagcttcag acccgaagtt ttccgaaacg acgcaaaaat cctattcatt
    18001 ctgatgctaa ttaccctgat ggctgtaatt tcgatttaca taataaagag tagccttttc
    18061 agcatataca tagtccccct ggtaattgtt ccaatttata tcctcacatt cttcgactcg
    18121 cggctagcgc tattcactca tctggtaacc atattcctgg taggattttt tgtacccaat
    18181 agcttcgagt tcgtttttat caactttatt gcgggaatgg tggccattat aagcatgacg
    18241 aacttctacc gcagggggcg attgtttgtt accgtgggca tggtatatgc tacctacgcc
    18301 ttactcctcg tgggacttgc agctgttgag gagggtaaca taaaagattt tgagagcatt
    18361 aaccttcttt ggtttgctat aaacgcccta ctgcttctgg cgctatacca gctggtttac
    18421 ctatttgaaa ggatttttgg cttcctctcc gatgcaacgc tcatcgagct atccgatacc
    18481 aacctcgatt tacttcgaaa gctggccgaa gttgccccag gcacctttca gcactccctt
    18541 caggtggcca acctggctga aacagcaatt caacatgttg gcggaaaccc cctgctggtt
    18601 agggctggag ttctatacca tgatattggg aaaatggcca acccgttgta cttcatcgaa
    18661 aaccaaccat acgacttcaa cccccaccga aacctggatt ttaccgaaag cgcatcaatc
    18721 attatcaacc atgttaccga gggtgtcaga attggccgca aggccggact tcccgaccag
    18781 ctgattgact tcatacaaac ccaccacggc acctcaaagg ttagatactt ctaccagcga
    18841 cacctcgata agtttgacaa cggcgctgcc gatgccgtaa agtttaccta ccctggccct
    18901 attccgccca gcaaggaaac agccattctg atgatggctg atgcagtgga agccgcctca
    18961 aggagtatga agaatgtgac tgaggaaaac ataaaggagc ttgttgacac gcttataaac
    19021 cagcagctgg ccgatggtca gtttaacgat gccaacatca cctttaagga tatctcgacc
    19081 attaaatcgg ttttcaagaa gaagctgctg aacatctacc acgcccgaat agagtacccc
    19141 gagactaagg ctaacgagta atttttttac tttcggatag tagtaggttg attctcgcag
    19201 gcacttatgg cccacggtaa caaccataaa agcaacataa ccaagcgaac taggccgaaa
    19261 aattttcaaa acccggtatg ccctcaatct taactgggcg accctgtttt aaccctactc
    19321 ctataacctt cgcctttact gccgttgagc catcgggtat ggctatcacc tcctgatcaa
    19381 acaccaaccg taaatgcccc tcacgacgag ctgataccct taccttaaat ttatcaccgc
    19441 tggcgagcga acgtttgtag tctatctcta ttcgggtaac caccaaatct ataccctcag
    19501 tgtgaaggcg agcaaagtca ataccccagc tgagcaaata tttatgacgg gcatgctcca
    19561 ggtagttcag gtaaactgcg ttgtttacaa ttccctcaag gtcgcactcg tagtcccgaa
    19621 cctccatctc tagttcgaac agctctgcta atttgtccat ggtatgtaac aaatatttat
    19681 acggccattg aataacactc tgaagagtag cgcaaatttg gtaaaaaaaa tcagtttccc
    19741 tcaaccatgt actccttaag cttctccgat ttagggttat tgaagagctc atcgggtgtt
    19801 ccctgttcaa taatttcacc attatacata aaaattacat ggtcggccaa ccgaatagcc
    19861 tgcctaagta catgcgttac caggataatg gtgtaatttt ccttgaggtt tcggaacagg
    19921 ttttcgataa ttttactgga aatggggtca agggccgagg ttggctcatc ggccaggatt
    19981 attcggggtt tcaccgccaa acctcgcgcc aaacaaagcc tttgctgctg accaatggag
    20041 aggctggtgg ctggagag
//
LOCUS       DAOPHX010000033        20026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_2910_length_20026_cov_6.385058, whole genome shotgun sequence.
ACCESSION   DAOPHX010000033 DAOPHX010000000
VERSION     DAOPHX010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 20026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20026
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2910_length_20026_cov_6.385058"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..213
                     /locus_tag="PK502_03970"
     CDS             40..213
                     /locus_tag="PK502_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00008.1"
                     /translation="MAELVLLLADSPNFKNGFKERHRPASRKTSAGGVKKNLLFEALS
                     GARRKFRFFSRQA"
     gene            complement(868..2187)
                     /locus_tag="PK502_03975"
     CDS             complement(868..2187)
                     /locus_tag="PK502_03975"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HPH00009.1"
                     /translation="MHLNSKILMLAMLIPCLTNRCSCNAYSQPVKWSLWDGIALENFS
                     AQSIQSVQVYRTGGELNNPVVELNNADETLTLEFDDITGSYIHYDYTLIHCGWDWKPS
                     GLLFTEFADGFEFNTIDPYGYSTGTILSYTHYNLTLPNEKVSFRISGNYILRVVDSNN
                     HDSIVIQQRFMVVEPIISMNAMVRLPANPKLQTTSQQLELEVLHSPLGNIDPTTDIVT
                     LIAQNNPTHPSMRCKPTFIDRGFTRYTDPDALVFSGGNEFRHIDLKSLHYQGQSISNI
                     AIRDNEYHVQLAPDESRSIQKYSDNPDINGKFIVKRDDSYDSDIEAEYVWAYFTLQVN
                     EDVSEDVYVFGELSGWTVNKGYRLNYNAVTRAYEQRILLKQGYYNYQYAMLDGATGSF
                     YPYVFEGDFSQTENIYLVTVYYRQPGVRYWRLVGIKTLNSRYSDKTR"
     gene            complement(3033..5096)
                     /locus_tag="PK502_03980"
     CDS             complement(3033..5096)
                     /locus_tag="PK502_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPH00010.1"
                     /translation="MRISTYIALIATGIIALLPLRSAYPQGIMDSQQVIADIIEDLTS
                     KAETDEDYSQLAEDLLELSENKINLNSAKKDDLLKLIFLSDFQVQSLLDYRDSTGSIL
                     SIYELQAVPGFDLVDVQRLLPFVKVENAEHAVTASELLGGRNEISIRFKTPLETSAGY
                     SDSYSAASRYTGSKNSYYTRYTYSGGKHFQVGLIAEKDAGEPMFDGTFKTGFDYLSGN
                     IVLSDVGRVKKLVVGDYHAEFGQGLTFWNGISFGKSGNVLSGHKRGRGLVRHSSAYES
                     QYLRGVGLTMPVLKADLTIFGSYRHIDAGIADTLDDGDLAFSSLPETGYHRTTSEIAN
                     RNTLQEMVAGANLTYNLRAVKTGLTFFHSEIEGVNAKTLAIYQLEPIRATKNALGMNV
                     DAYLGKHLLFGEVAAELPTMKLAGIAGGFFRLTNTVQLAMIGRMYARDFNPRYTNGFA
                     EGSGVSNERGLYTGLSVLPAKGWKLTTYVDIFKFPWLRYQVNAPSEGREFMAQSDAVI
                     AENFSLTIRYRGKEAPKNLSGSTAQVTPVVGQTTQSLRLQFGYLPLETVRLKTTLEAS
                     AFSNDSLATEKGYLVAQDISLNPKRFPLSVNARFAVFDTPSWNSRIYSYESDMLYSFT
                     VPAYYSKGTRLFVMVKYSLKNRLDVWLRYSQTYFSELDELGQGADLVEANTRSEIKAM
                     VRFKF"
     gene            complement(5099..5929)
                     /locus_tag="PK502_03985"
     CDS             complement(5099..5929)
                     /locus_tag="PK502_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00011.1"
                     /translation="MSGRVVTTMVILMLFSTAKASIPDKPTGGQGAAMGQAWVALNDS
                     WAGFRNQAALAVNRNFWAGIHHENRFLTSELGLSTLGVLIPVSPGTFGVDISHFGYSQ
                     LNTTRIGLSYGMKFSEKFMAGIGLAYHRMQVAGEYKDRNAFTVEGGILYAPVEKLTLG
                     AYIFNPTRSSFDDSQSLPPIIGAGATFRPSQKVVLTLQVDDDTESSPNVRGGLEFTAT
                     KNFCFRLGYSSGNPEGLTGGFGWMVKDINFDLSFGYHRILGYTPQLSVSYCPGSKKNS
                     "
     gene            complement(5957..6469)
                     /gene="purE"
                     /locus_tag="PK502_03990"
     CDS             complement(5957..6469)
                     /gene="purE"
                     /locus_tag="PK502_03990"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPH00012.1"
                     /translation="MTPKVSIIMGSVSDMPIMEDAAKFLDELNIAFEINALSAHRTPE
                     MVMEFAQNAHTRGVKVIIAGAGGAAHLPGVIAASTPLPVIGVPIKSSNSIDGWDSILS
                     MLQMPAGIPVATVALNGARNAGILAAQIIATGEPEMLKVVVDFKKSLGEKVVKANQEL
                     ANVKFKCKTN"
     gene            6648..7187
                     /gene="hpt"
                     /locus_tag="PK502_03995"
     CDS             6648..7187
                     /gene="hpt"
                     /locus_tag="PK502_03995"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPH00013.1"
                     /translation="MKQVTLKDRTFQLSYPEADIQKDIDVTASKINWDYRNASDIPLF
                     LCILNGSFMFAADLLKRITFPCEVSFIKLASYQGDTSTGVVNHLIGLNESISGRTVII
                     VEDIVDTGVTIAQLIDELQKMNPKEVKVATMLHKPDAYKGSIKIDYIGRSIPNDFIVG
                     YGLDYQGLGRNLPDIYTVI"
     gene            7496..8071
                     /locus_tag="PK502_04000"
     CDS             7496..8071
                     /locus_tag="PK502_04000"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPH00014.1"
                     /translation="MLNIILFGPPGAGKGTQSQKIIEKYNLQHLSTGDMLRSAIQQNS
                     PLGLKAKEFMDKGQLVPDEMVIDLIASRIDANGSTKGFVFDGFPRTTLQAAKLDRMLE
                     QRKTPITLLISLEVDYDELIARLVNRGKVCDRTDDQDENVIRGRIDVYNKTTLPVLDY
                     YKAQGKLRSVSGMGSIDEIFERICHIIDCVV"
     gene            8111..9103
                     /gene="obgE"
                     /locus_tag="PK502_04005"
     CDS             8111..9103
                     /gene="obgE"
                     /locus_tag="PK502_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPH00015.1"
                     /translation="MSNPNFIDYVKIYCRSGQGGAGSVHFRREKYIPKGGPDGGDGGR
                     GGSIYIRGNQQLWTLLHLKYKRHIFAGDGESGSGARCTGKDGKDEIIEVPVGTIAKNV
                     ETGEVIAEVVSDGQTKIIMKGGRGGQGNWHFKSPTMQTPRFAQPGEPCEEGYVILELK
                     ILADVGLVGFPNAGKSTLLSVVSAAKPKIADYPFTTLTPNVGIVGYHDDRSFVMADIP
                     GIIEGAHEGKGLGLRFLRHIERNSMLLFMVPADSEDVLHQYSVLVNELRQYNPELLDK
                     KRALAITKCDLADEHVRKSISLNLPDVPSVFISSVTGEGIERLKDLIWSNINTD"
     gene            9107..9664
                     /locus_tag="PK502_04010"
     CDS             9107..9664
                     /locus_tag="PK502_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPH00016.1"
                     /translation="MVDFLLQLDTKLFLALNGWHSPFWDSIMLFASGKLSWLPLYLLL
                     VFFMIRRFGWKTLWWLLAAAVVVLLADQVTGHAFKYVYHRLRPCHNPDLSSVIHLVGR
                     CGGKYGFISAHAANSFGVATLVSFIFYKRWATIGMLVWATFVSYSRIYLGVHYPGDVL
                     VGAIVGSAIGIAVWLVAERLINRRN"
     gene            9778..11628
                     /locus_tag="PK502_04015"
     CDS             9778..11628
                     /locus_tag="PK502_04015"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HPH00017.1"
                     /translation="MDTELKINEVEVKPHELKVSVNVKEINKRIENIHRYLNSHKEYL
                     NRLNVFPVPDGDTGLNLTLTFQGAMASMQGMENESMLPGEYLKNFTEQMLLNSRGCSG
                     VIFSLFAQGVAQVLENNDFSNENLHRAIANGFKNAWDGTENPREGTMLTLMREFRDIF
                     GKYIQEEKNAAMVISKSIPYLMEVLDRTPEMLPVLKQAGVVDSGGAGFIIILQGIDRE
                     LKHNGSGFNSLSIATLLNISRTTRKLLRMRKSHVRKSSLTPLITNIDDSKIHNSRLRE
                     ILQNARKVINTWNGSRKLPGQEKVISDLEEMGNLWDSDIKLKYCTEFVLEANAINSKE
                     ELREIIGHYGDSLIIVNSGNRYKVHIHTNKPDAVFADAGRYGELVFTKVDDMKKQHRN
                     FVSDDVIEYERDKSILCIVSGSGFADILKNFGADDVLCYGKNKPSVNQLVKKIDGLKA
                     KNVIAAADDSDILMALKYAVSLCKSNVYIVESNNPISIIAMMLGISKEFDIATMFESA
                     MSNLNSITYCEIARSVRNVEADGFTVKKRDFFAVHNKKIIISRTNLEQLVVDVVEKLN
                     PGASLVTIYKGIPSRSDRSLLPILREKFPNLEFEEYYGGQSKFNYYISFE"
     gene            11641..12519
                     /locus_tag="PK502_04020"
     CDS             11641..12519
                     /locus_tag="PK502_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPH00018.1"
                     /translation="MSQKIFVISADNLGLSRDQVDYPDLELLRFPVFVGDKEYRQDEV
                     HNAKWLLQKYTNEQVVAKSSTLVKQEMVDIVERVRSSYDMIVQVVMGSGMSSASFVVA
                     ENVRKMYEGIIPIVNVDSSQVINGVGNVLLGVIDLIKSNPNLSPEEIAQKSKDVVQNT
                     FTYFIFPDLNYLYRGGRIGKAKSLVGTIMKIIPIIGMLGDNGEGVMVPVGKGRTFKQV
                     NTQLVELIKAKMAEKSVSTIKRMNLTHFGGNDEAVAELKEMVGKLPCNDFIIGTPNLV
                     EAVYAGPGAYCLSMSL"
     gene            12532..13149
                     /locus_tag="PK502_04025"
     CDS             12532..13149
                     /locus_tag="PK502_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HPH00019.1"
                     /translation="MNSYIVNLICLVVGYLCGSVLFAHIITRLVVGKEIRELGNNNPG
                     AYNVFNNAGKFWGVFTGILDGLKAFVPMLVAHRYFNVSNTGIGLIGVGAVIGHGYPLF
                     FKFKGGRAASSLMGMFLFFIPYELLAAFAIDLILLYTIVKKDFGFWGPFGIIAIAATL
                     CLFFNHPVEVKILIWVGGLLGVYFNRKFIPLRTKQLLNKAPEDDN"
     gene            complement(13219..15252)
                     /locus_tag="PK502_04030"
     CDS             complement(13219..15252)
                     /locus_tag="PK502_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPH00020.1"
                     /translation="MNRKIMALLLMAATGFSTGGCKSGNEQAEPFKWQVDQFADLRIM
                     RYQIPGFDSLTPKQRVLVYYLSEAAKCGRDIIWDQNYKFNLDVRSVLEGVYQGYKGNR
                     NDPRWGSFMVYLKRVWFSNGIHHHYSTDKIMPDFDRSYFAELVGNTPSEMLPKKFGGV
                     DSILAHFTPIIFDADVAPKRVNQLAGSDLVTTSACNYYEGVTQAEAEEFYSRLANPND
                     STPISYGLNSKLVKKNGKIEEQVWYANGMYGKAIGKIVFWLEKAATVAENEYQQKIIE
                     TLVDYYKTGDLRKFDEYNILWLSDTQSKVDFVNGFIETYGDPLGYKGSWESVVNFRND
                     EATHRTEVISANAQWFEDHSPIDSIFKKKEVKGVSAKVITVAQLGGDCYPSTPIGINL
                     PNADWIRKQHGSKSVTMDNITYAYNQASLGNGFMEEFAWSDEEVALNKQYGYLADCLH
                     TDLHECLGHGSGQLAPGVKGDELKNYGSPLEEARADLFALYYIMDPKMVELGLMPNLD
                     VARVQYSNYIRNGLMTQLTRVEPGKNIEQAHMRNRQLIAQWCYEYGKDENVVERKNRN
                     GKTYFVVNDFQKLRQLFGSLLREIQRIKSSGDYESGKKLVESFGVKVDPALHHEVLER
                     YSKLNLAPYGGFINPDLVPVVQNDSIVDVRVDYPEDYARQMLEYSERYSFLTK"
     gene            complement(15289..16215)
                     /locus_tag="PK502_04035"
     CDS             complement(15289..16215)
                     /locus_tag="PK502_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00021.1"
                     /translation="MRKVILINVAAALVILPLVGFGQEVNANFFSTREDSLSKLLSLI
                     VSETSTTRRDSLNRIFHENFKNTLEHDGAIDYPFSSLKHVGMLTSSDQKVRIITWNIP
                     QTGGYHRYFGFVLVRLDGDKSRLYALTDSREMVKDPQHERLPADRWLGALYYQLAELP
                     YGSEKQYVLLGFDYNNLFTSKKVIEVLTLSPQGEPVFGLPVFNVDNKQLLSRIVFEFS
                     ARAGFNLKYYDEQKIIAFDHLSPSQPEFTGDFQFYGPDASIDGFAFENGKWVYVRDLD
                     MRNPSREKVNPVDAPEKIDEPSFLYKPQQKRK"
     gene            complement(16219..17412)
                     /locus_tag="PK502_04040"
     CDS             complement(16219..17412)
                     /locus_tag="PK502_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPH00022.1"
                     /translation="MQNIRQLFLSHVAQTSDAPLMLEVSRAEGVFLFGPSGEKYYDLI
                     SGISVSNVGHANPRVVKAVQDQASAYMHLMVYGEFIQSPQVLLAAKLSSMLPQTLSSV
                     YFVNSGSEAVEGAMKLSKRFTGRTEIISFRNAYHGGTQGALSVMGVESQKRSFRPLLP
                     DISILEFNSIADLARITNRTACVIIEPVQGEAGIRVADGNFITELRRRCSEVGALLIF
                     DEIQTGMGRTGKMFALERFGVTPDILLLAKALGGGMPLGAFISSQKIMNCLMENPSLG
                     HITTFGGHPVSCAASLAAIDVLTGDRLPESVPEKEQIFRDILSKHPLVKEFRSFGLLM
                     AIELGSFEKVSALIKYGVEHGFVLDWFLFCNTAIRLAPPLCITKEQCEEVANLIYQGL
                     NEINR"
     gene            complement(17431..18141)
                     /locus_tag="PK502_04045"
     CDS             complement(17431..18141)
                     /locus_tag="PK502_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPH00023.1"
                     /translation="MNCIQVDDDELSRRVIEDYASKIDFIRLTGSFPNAVDALNFLKN
                     SLEPVNLIFLDIEMPEMSGIDFLEATSQEPQVIIISSKEKYALKAFDYSVTDYLLKPI
                     TFPRFTKAVSKAYDKFNQQRSPYDDDKEIFIKKNNMLIRVKYSDILWIEALENYIVIN
                     TVNERFTIHLTMKSIENQLSSVRFKRIHRSFIVNVGKIESIEDNATVIRTPEGRKIIP
                     IGKSYRDKLLSEINLINK"
     gene            complement(18195..18911)
                     /locus_tag="PK502_04050"
     CDS             complement(18195..18911)
                     /locus_tag="PK502_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPH00024.1"
                     /translation="MSGIDHLIQSDRQFRLQLTSYLSDLVLERRLNLLREVLSRRTRY
                     ITVVLEDLYQPQNASAALRSCECLGIQDVHIIENRNSFNVNPEVDMGASKWLTIKKYS
                     GNKSITQEALQTLRSSGYRIVATSPHSLGCSIRDFDTEKGKFAILFGTELLGLSEEAM
                     SLSEEFITIPMYGFTESYNISVSVAITLYELVHRAKANPAYPLSEYEKEVVLLDWLRK
                     SVKNCHIVEKRFVAEYNKLH"
     gene            complement(19188..19460)
                     /locus_tag="PK502_04055"
     CDS             complement(19188..19460)
                     /locus_tag="PK502_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPH00025.1"
                     /translation="MNKAQLIDAIAAEAKVTKADAKKALDAFIKTTGTALKKGDRVAL
                     VGFGSFSVAKRSARTGRNPQTGKPINIKAKKVVKFKAGSELNGKVQ"
     gene            19927..20015
                     /locus_tag="PK502_04060"
     tRNA            19927..20015
                     /locus_tag="PK502_04060"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19961..19963,aa:Ser,seq:tga)
BASE COUNT         5198 a         4373 c         4785 g         5670 t
ORIGIN      
        1 caagggtaaa ggataaagga taaaaaaata gtctgggtaa tggcggaact tgtcctccta
       61 ctggcggatt caccaaactt taaaaatggt tttaaagagc ggcatcgtcc ggcgagccga
      121 aaaacaagcg caggcggcgt taaaaaaaat ctgctgttcg aagcgctgag cggagcgagg
      181 cgcaagttca gattttttag ccgccaagcg tagctttttc gtgcgaggcg ggcgcagcct
      241 tgactttttt tgcatacttt tttgtgtcaa ccgaaccgcc gtcaggcggt gagctcgccg
      301 aacaccttaa aaaatgagct aggggtgagg caacaaaaaa agtatgtggg atttggggca
      361 aagccccaag atttcatgat aataaactta tccagctttt aacaagctca caaattcatt
      421 tcgccctttc agggcttaat acatcttact tctatacttc aaggggctac accccttcct
      481 agctattccg ccccgttggg gcttaagaaa aggataaagg gtaaaggata agaatattca
      541 agattaagaa cttttttatt agaagagttt ttaagccccg aaggggctga atctttgtag
      601 tcccgggcgt aagcccgggg ttgatgaggg tcggcggtgg ttggcgatgc aagtagcggc
      661 tttctcaagg tgcaatttca atcctttgca tcgcaaagga gaaaatttta ccaggagcaa
      721 ggtaaaatta aacataaaag aaaatctttc cacctccctt tacccctcga caagttcgga
      781 attcttaact ctttacctgt tcaaagggtt cattttgaac agccgctgga tgctgaaatc
      841 aaacgatgtt gcctatattg cttctaatta ccttgtctta tcgctgtacc ttgagtttag
      901 agttttaatc ccaacaagtc gccagtatct tactccgggt tgcctgtagt aaactgttac
      961 caagtagata ttctcggttt gagagaagtc tccctcaaaa acatacggat agaaactacc
     1021 ggttgctcca tcaagcattg catactggta gttgtagtat ccctgtttaa gcaggatgcg
     1081 ctgttcgtaa gctcttgtaa cggcgttata gttgagcctg taacccttat ttacagtcca
     1141 accgcttagc tcaccaaata cgtaaacatc ctcgctgacg tcctcgttga cctgaagggt
     1201 aaagtaagcc catacgtact ctgcctcaat atcgctatcg tagctatcat cacgctttac
     1261 aatgaacttg ccgttgatgt ccggattatc cgagtacttc tgaatgcttc tgctttcatc
     1321 aggagcaagt tgaacatggt actcgttgtc cctgatggct atattactaa ttgattggcc
     1381 ctggtagtgt agcgatttta ggtcgatatg cctaaactca ttccctccgg aaaaaaccag
     1441 cgcatcggga tcggtgtacc tggtaaatcc tctgtctata aaggttggct tacagcgcat
     1501 agagggatgg gtggggttat tctgggcgat gagggtaaca atgtcggttg tgggatctat
     1561 attgccaagg ggtgagtgta gtacttccag ctccagctgc tgcgaagtgg tttgcagctt
     1621 cgggttggca ggtaaccgaa ccatggcgtt cattgatata attggctcaa caaccataaa
     1681 gcgctgctgg attacgatac tgtcgtggtt gttgctatcg acaactctca gaatatagtt
     1741 tccggatatt ctaaagctaa ccttttcgtt tggaagtgtt aggttgtaat gggtatagga
     1801 taggatggtg cctgttgaat aaccataagg atcaatagtg ttaaactcaa aaccatcggc
     1861 aaattcagta aaaagcaaac ccgatggttt ccaatcccaa ccgcaatgga taagggtata
     1921 gtcgtaatgg atgtagctgc cggtaatgtc atcaaactcc agggttaggg tttcatcggc
     1981 attatttagc tctacaacag ggttgttaag ttctccgcct gtccggtaaa cctgcaccga
     2041 ttgtatggat tgtgccgaga agttttcaag tgctatgcca tcccataaag accattttac
     2101 tggctggctg taggcattgc agctacacct attggttaag cagggtatca gcatggccaa
     2161 cattaaaatt ttgctattga ggtgcattga ccggtgggtt tgtggtttac tggagcaaag
     2221 ttatttttta tttgctttgt ttgaatgttg acaaagcatc taaggctata gaattcgttt
     2281 gtttgcgtac gacccattag acattctgat ggatacctta aaatagttaa cagcagcaaa
     2341 atgaaagtca tttttgattt aaaagttgat gcaaccttta accattatta acagcacttt
     2401 aacgtgcgtt agctattgat gggcttatat ttgcagtgta tttaaaaaca aaagtttctc
     2461 ataggctttt ggttttagag ttaggtttag gtttggtttt aggtttaggg gttagaaaca
     2521 ggggaggggc gatgagcccc tcttttgcgt ttatcaagat gtatgcaata ggggtaaatg
     2581 gtattaaatt taaaatgcca taagactctg ggtttaaatt ttttctcacc acagagagca
     2641 cggagaatca cggagggatt taaatttttt tctctgcgta cctcagtgtt ctccgtggtt
     2701 gagtatttct caccacagag agcacggaga atcacggagg aattttaatg aattttcttc
     2761 gtttatatcc gtgtcctttg tggtaaaaag cgtttgctat tgcttaaatt aagcctactt
     2821 tagtaaacac ctaacctatt gaaaatggtt tcttcaaagg attgataggt actttggaga
     2881 taaaaatatt ggtagctgca tgaattgaaa tacttcagaa aggtataggg caaaacaagc
     2941 ttcgccgcta cactaaaact acggtttgga attaataagg aatggaataa caatttagca
     3001 attttattgg ttggttatag cgtcaagtag ctctaaaatt taaacctcac catcgcctta
     3061 atctcgcttc gggtattagc ctctaccagg tcggcgccct gtcccagctc gtccagctcg
     3121 ctgaagtagg tctgtgagta ccgcagccac acatccaacc gattttttaa gctgtacttg
     3181 accattacaa aaagtcgggt tccttttgag tagtaagctg gcactgtaaa agagtagagc
     3241 atatcgcttt cgtaggagta aatgcgacta ttccacgatg gggtatcgaa tacggcgaag
     3301 cgtgcattta cacttaaggg gaaacgtttg gggtttaagc tgatgtcctg tgccaccagg
     3361 tagcctttct cggtggcaag gctgtcgttg ctgaatgctg atgcctcaag tgtggttttt
     3421 agccttaccg tttcgagggg aaggtagccg aactgcaacc ttaggctctg cgtggtttgt
     3481 cccactacag gtgtaacctg tgcagttgag cccgacaggt tctttggtgc ctccttgccc
     3541 ctgtacctta tggtgaggga aaagttttct gctattacag cgtccgattg agccatgaac
     3601 tccctaccct ccgatggggc attcacctgg tatctcagcc acggaaattt aaagatatca
     3661 acataggttg ttagcttcca accctttgcg gggagaacgg atagcccggt gtataaccct
     3721 ctttcgttcg atacgccgct tccctcggca aatccattgg tgtatcgggg gttgaagtct
     3781 cgggcgtaca tcctgccaat catggctagc tgaacggtgt tggtcagccg gaaaaaaccg
     3841 cccgcaatac ctgcaagctt catcgttggt agctctgctg caacctctcc aaacagcagg
     3901 tgtttaccca aataggcatc aacgttcata cctagagcgt tctttgttgc gcggatgggc
     3961 tccagctggt agatggcaag cgttttggcg ttaaccccct caatttctga gtgaaagaat
     4021 gttaagcccg ttttcactgc cctgaggttg taggttaggt tcgcaccggc taccatctcc
     4081 tgtaaggtgt ttcggtttgc aatctcggag gttgttcggt ggtagccggt ttcgggaagc
     4141 gacgaaaagg ccagatcacc atcatcaagc gtatcggcta tgccggcatc aatgtggcgg
     4201 taggagccaa aaatggttaa atcggccttt agaacaggca tggtaagacc aactccccgt
     4261 agatactgac tctcataagc tgacgaatgt cgcaccagcc cacgtccacg tttatgcccc
     4321 gaaagaacat tacccgattt tccaaatgag atgccgttcc aaaaggtgag tccctgccca
     4381 aactcggcgt ggtagtcgcc caccaccagt tttttaaccc tacccacatc cgaaaggact
     4441 atattccccg agaggtagtc aaacccggtt ttgaatgtcc catcaaacat gggttcgcca
     4501 gcatccttct cagctattaa acctacctga aagtgctttc ctccgctata ggtgtaacgg
     4561 gtatagtagg agtttttgct ccccgtgtac cgtgatgctg cggagtatga atcggagtac
     4621 ccggccgatg tttccaaagg cgttttaaac cgaatgctga tttcatttct accgcctagc
     4681 agctcgctgg cagttacagc atgttcggca ttttcgacct ttacaaaagg aagtaatctt
     4741 tgaacgtcaa ccaaatcgaa acccggaaca gcttgtagct cgtaaatgct aagtatggag
     4801 ccggttgagt ccctgtagtc cagcaggctc tgtacctgaa aatcgctgag gaagattagc
     4861 tttagcaggt catccttttt tgctgagttc aggttgattt tgttttcgga cagctcaagc
     4921 aaatcttcag ccagttggga gtagtcctcg tcggtttctg cttttgaggt aagatcctca
     4981 ataatgtcgg ctatcacctg ctgggagtcc atgataccct gcgggtatgc tgacctgagg
     5041 ggaaggaggg caataattcc ggttgcgatt aaggcaatat atgttgaaat cctcatggct
     5101 aggagttctt tttgctaccc gggcagtagc tcaccgatag ctgcggggta tagcctaaaa
     5161 tcctgtgata gccaaaggaa agatcaaaat ttatgtcctt aaccatccat ccaaaaccac
     5221 cggttagccc ttcagggttt ccagatgagt agccaagccg gaagcagaaa ttcttagttg
     5281 ccgtgaactc gagaccgccc ctcacgttag gactactttc ggtgtcatca tcaacctgaa
     5341 gggtaagtac aactttctgg ctgggtctga atgtggcccc tgcaccaatt attggcggca
     5401 gcgattggct atcatcgaat gaggatcggg tagggttaaa gatgtaggcg ccaagggtta
     5461 gtttctctac cggggcatat aggattccac cttcaactgt gaaggcattc ctgtccttgt
     5521 actcacccgc cacctgcatc cggtggtagg caagtccgat tccggccatg aacttctccg
     5581 aaaatttcat gccatacgac aagccgatgc gggtggtgtt tagctggctg tagccgaaat
     5641 gcgagatgtc aaccccaaaa gtgccaggcg aaacaggaat caggactcca agtgtgctca
     5701 gcccaagctc gctggtcaga aacctgtttt cgtggtggat acctgcccag aagttgcggt
     5761 ttacagctaa cgcagcttgg ttcctgaacc ctgcccagct gtcgttcagg gcaacccatg
     5821 cttgccccat agcagcccct tggcctccgg ttggcttatc gggaatggag gctttagctg
     5881 ttgaaaacag cattaggatg accattgtgg taaccactct cccggacatg gcaggtccac
     5941 cttggtgtgg aagtttctag tttgtcttac acttgaattt cacgtttgcc aactcctggt
     6001 tggcttttac caccttttcg cctagcgatt tcttgaagtc aaccactacc tttaacattt
     6061 cgggttcgcc ggtagcaatg atttgggcgg ccaaaatgcc ggcgttgcgt gccccgttga
     6121 gggcaactgt tgccacagga atgccggcag gcatttgaag catggacagt atcgaatccc
     6181 aaccatcaat agagttggac gacttaatgg gaacaccaat aaccggcaat ggggttgatg
     6241 cggcaattac acctggcagg tgtgctgcac cccctgcacc ggcaataatc acctttacgc
     6301 ctcgggtatg agcgttttgg gcaaactcca tcaccatctc gggtgttcgg tgggccgaca
     6361 gggcgttaat ttcaaaggct atgttcaact cgtccagaaa ctttgcagcg tcttccataa
     6421 tcggcatatc cgaaacgctg cccataataa tgcttacctt tggtgtcatt tataaatggc
     6481 tgattagttt tcaacaaaga taaaattttt ttcgaaatat gccttcctgt ttgctgccat
     6541 gttcaataaa tcccaattta cttatggttc agttttaaat ttaaaaaatc gggctaattt
     6601 tgttgcataa ttactgagta atggatgata atagctataa aagtcaaatg aagcaggtta
     6661 cgctgaagga taggacattc cagctaagct accccgaggc agacattcag aaggatattg
     6721 atgtaacggc ttccaaaatt aactgggact atcgaaatgc ttccgatatc ccgttatttc
     6781 tttgcatact caatggttca ttcatgtttg ccgccgactt gctgaagcgc attacttttc
     6841 cctgtgaggt ctcgtttatt aagttggcct cctaccaggg cgacacctca actggagtgg
     6901 taaatcattt aattggctta aatgaaagca tttccggtcg cacagtgatt attgttgagg
     6961 atattgtgga taccggggta actattgccc agctcattga tgaacttcag aaaatgaacc
     7021 caaaagaggt aaaggtggct actatgctcc ataaacctga tgcctacaag ggaagcataa
     7081 aaatagacta tattggtcgt tctatcccca acgattttat tgttggctat ggcttagact
     7141 accaagggct tggccgtaac ctacccgata tttacacggt tatttgatgg ttttcttgga
     7201 gggttcgggt gaatgttgag gcctgaaagt taatgttggg acgctcgaac agcgagccgt
     7261 aagccctgaa gggtgaaagt tattgggaaa gggaagggga aatatgctgt taaacttggg
     7321 ccagctaagt gttaaatggt tggggatgaa ttatttgaat ctattacagc cgagattttt
     7381 tgaatccgtc aggagccgga caagttccgt caggaggcag ataggtttat tatcttttat
     7441 cctttaacct ttatcctttt accttgattt ttaaccttaa tgctaacata ccgccatgct
     7501 taacatcatc ttgttcggac ctcccggggc cgggaaagga actcaatcgc agaagataat
     7561 tgaaaagtat aaccttcaac atttatcaac tggcgatatg ctgagatcgg ccattcagca
     7621 gaacagccct ttaggtttaa aggccaagga gtttatggat aaaggccagc ttgtacccga
     7681 tgagatggtt attgacctga ttgcctccag gattgacgct aatggtagca ccaagggttt
     7741 tgtattcgat ggttttcctc ggacaaccct tcaggcagcc aagctcgacc gtatgctgga
     7801 acagagaaaa acgccaatca ccctgttaat ttcccttgag gtggactacg atgagctcat
     7861 agcccggctg gttaacaggg gtaaagtttg cgaccgaacc gacgaccagg acgaaaatgt
     7921 cattcgcggt agaattgacg tttacaataa gacaaccctg ccagttctgg attactacaa
     7981 ggctcagggg aaactgcgta gcgtgagcgg catgggctct atagatgaaa tttttgagcg
     8041 aatctgtcat attattgact gcgttgttta gcataatgct tccgcaacca cacattatac
     8101 ctgttgcaag atgtccaatc ccaactttat tgactacgta aaaatctatt gccgctcggg
     8161 tcagggcggc gctggaagcg ttcacttccg ccgcgagaag tatattccta agggtggccc
     8221 tgatggaggt gatggcggaa ggggtggctc catctacata aggggaaacc agcagctgtg
     8281 gacgttactt catctgaaat ataaacgaca tatttttgca ggtgatggcg aatcagggtc
     8341 aggggcaaga tgcactggta aggacggaaa ggatgaaatt attgaagttc cggttggtac
     8401 tattgcaaaa aatgtcgaaa caggtgaggt tattgccgag gtggtgagcg atggccaaac
     8461 taaaataata atgaaaggcg gtcgtggtgg gcaagggaac tggcatttca agtcgcctac
     8521 catgcaaacg ccccgttttg cccagccagg tgaaccctgc gaggagggct acgtgattct
     8581 ggaacttaaa attttagccg atgtggggct ggtaggattt cccaacgccg gcaaatcaac
     8641 cctgctatcg gtggtaagtg ccgcaaaacc taaaattgcg gactatccat ttactaccct
     8701 tactccaaat gtaggaattg ttgggtatca cgatgatagg tcatttgtta tggccgatat
     8761 tcctggaata attgagggtg cgcacgaggg aaaaggttta ggacttaggt ttttgagaca
     8821 catcgagcga aactccatgc tacttttcat ggttcctgct gattccgagg atgtgctgca
     8881 ccagtacagc gttctggtca acgagcttcg gcagtataac cccgagctac tcgataagaa
     8941 acgtgcttta gctattacca agtgcgacct ggctgacgag catgtccgaa aatcgattag
     9001 cctaaaccta ccagatgttc cttctgtttt catctcatcg gttacgggag agggaattga
     9061 gcggctaaag gacttaatat ggagtaacat taatactgat tagcaaatgg ttgactttct
     9121 gctgcagctt gacacaaaac tatttctagc gcttaatggt tggcactcac ccttttggga
     9181 cagcataatg ctttttgctt cgggcaagct tagctggttg ccgctctacc tgcttctcgt
     9241 tttctttatg ataaggagat ttggctggaa aacgctttgg tggctgttgg ccgctgcggt
     9301 tgtcgtgctg ttggctgatc aggtaacagg gcacgccttt aagtatgttt accataggct
     9361 gcgtccttgc cataaccccg acctaagtag tgtaattcac ctggtaggac ggtgtggggg
     9421 gaagtatgga ttcatctcag cccatgccgc caattcgttt ggtgtggcca cgctggtaag
     9481 tttcatattc tataagcgct gggctactat tggaatgttg gtttgggcaa cttttgtctc
     9541 ctacagccga atatacctcg gcgttcatta tcccggcgat gttctggtgg gagctattgt
     9601 aggttcagca ataggtattg ccgtgtggct ggttgcagaa aggctcataa accgaagaaa
     9661 ctagggatat tttcaattta aatattttta tttttgccgg aggacttacg ttagaagttt
     9721 taaaagttta tccgcacatt cataaaagca aaccaaattg acaattagct ggttgttatg
     9781 gatacagaac taaaaataaa tgaggtagag gtaaaacctc acgaattgaa ggtaagcgtt
     9841 aatgttaagg agataaacaa aaggattgaa aatattcatc gctacctgaa cagccataag
     9901 gagtacctga ataggctaaa tgttttcccg gtacccgacg gcgataccgg gttaaaccta
     9961 acgctaacct ttcagggtgc aatggccagc atgcagggta tggagaacga atccatgctt
    10021 cccggcgagt atcttaaaaa ttttaccgag caaatgctgc tgaatagccg gggttgttca
    10081 ggtgtgattt tttcattgtt tgcccaggga gttgcccagg ttctggaaaa taatgatttt
    10141 tcaaatgaaa acctccacag ggctattgcc aatggtttca aaaacgcctg ggatggaacc
    10201 gaaaatcccc gcgagggaac catgcttacc ctgatgcggg aatttaggga tatatttggc
    10261 aagtacattc aggaggagaa gaatgctgct atggtaatca gcaagtcgat cccctacttg
    10321 atggaggtgc ttgacagaac ccccgaaatg ctccccgtgc ttaaacaagc gggcgtggtt
    10381 gactccggag gtgcaggttt tatcatcatc ctccagggta tcgaccgtga gctaaagcac
    10441 aacggttcgg gttttaacag cttgtccata gctaccctgc taaacataag ccgaaccacc
    10501 cgtaagcttt tgcggatgag aaagtctcat gttcgcaaaa gttcccttac gccacttatt
    10561 accaatattg atgatagcaa aattcacaac tcccggttgc gcgaaattct tcagaatgca
    10621 cgaaaggtta taaacacttg gaatggtagc cgaaagttgc ctggccagga aaaggtaata
    10681 agcgatttgg aagaaatggg aaatctctgg gactccgaca taaaattaaa gtactgcacc
    10741 gaatttgtgc tggaggccaa tgccatcaac tctaaggaag aacttagaga aattattggt
    10801 cactatggcg atagcctaat tattgttaac agcggtaacc ggtataaggt gcatatccat
    10861 accaataagc ccgatgctgt ttttgccgat gctggcaggt atggcgagct ggttttcacc
    10921 aaggtggacg acatgaaaaa gcagcaccgc aactttgtga gcgacgatgt tattgaatat
    10981 gaaagggata aaagtattct ctgcatcgtc tccggcagcg ggtttgctga tattcttaag
    11041 aattttggtg ccgatgacgt gctgtgctat ggaaagaaca agccctcggt taaccagctg
    11101 gtaaaaaaga ttgatgggct aaaggccaaa aacgttatag cagctgccga tgatagcgac
    11161 atcctgatgg cgcttaagta cgccgtttcg ctctgtaaat cgaacgtgta tattgtggag
    11221 tcgaataacc ctatttccat tattgccatg atgcttggaa tttcgaagga gttcgacatt
    11281 gccaccatgt tcgaatcggc catgagcaac ctgaactcta ttacctactg cgaaatagcc
    11341 aggtctgtca gaaatgttga ggccgatggt tttacagtta aaaagcgaga tttctttgcc
    11401 gttcacaaca agaaaattat tatttcccga accaacctgg agcaacttgt ggttgacgtt
    11461 gttgaaaagc taaacccagg agcaagtctg gttacgatat acaaagggat tccttccagg
    11521 agcgatagaa gcctgctgcc aatccttagg gaaaaatttc caaacctcga atttgaggag
    11581 tactacggag gacaaagtaa gtttaactac tatattagct ttgaataaac tttaaaaact
    11641 atgagccaga agatatttgt gattagtgcc gataacctcg ggctttcgag ggatcaggtt
    11701 gactaccccg atttggagct gctcaggttc ccggtatttg taggggataa agagtaccga
    11761 caggatgagg tgcacaatgc caaatggctt ttgcagaagt acaccaacga acaggtggtt
    11821 gccaaatcat ccacgctggt gaagcaggaa atggtggata ttgtggagcg ggttcgttca
    11881 agctatgaca tgattgttca agtggtgatg ggaagcggca tgtcaagcgc ttcgtttgtg
    11941 gttgccgaaa acgtgcgcaa gatgtacgag ggcattatcc cgatagtgaa tgtggacagc
    12001 agccaggtga taaatggtgt tggtaatgtt ctacttggcg ttattgattt aattaagagc
    12061 aatcccaact taagtcctga agaaattgca cagaagtcga aggatgttgt gcagaataca
    12121 tttacctact tcattttccc tgaccttaac tacttgtata ggggaggaag aatcggaaaa
    12181 gctaaatcgc tggttggaac cattatgaaa attataccca ttattggtat gctaggtgat
    12241 aacggcgaag gtgttatggt tcccgttgga aagggtagaa cattcaagca ggtaaacacc
    12301 cagctcgttg agctgataaa ggccaaaatg gccgaaaaat ctgtatcaac cattaagcgc
    12361 atgaacctta cccattttgg tggaaacgat gaagcggttg ccgagctaaa ggaaatggtt
    12421 gggaagttgc catgcaacga ctttattatt ggtaccccga acctggtaga ggcagtatat
    12481 gccggacctg gagcttactg cttgtccatg tcactataaa tttttaatgt tatgaacagc
    12541 tatatagtta accttatttg cctggttgtg ggttacctgt gcggttcggt gttgtttgcc
    12601 catattatca ccaggctggt tgtaggaaag gaaattaggg agttggggaa caacaacccc
    12661 ggtgcctaca atgtttttaa caatgctggt aagttctggg gcgtttttac gggcattctg
    12721 gatgggctaa aggcctttgt tcccatgctg gtggcccata ggtactttaa cgtttccaat
    12781 actggtatag ggttaatagg agtaggcgcg gtgattggac atggctaccc gcttttcttc
    12841 aagtttaaag ggggtagggc agcctcatcg ctcatgggca tgtttctctt ttttataccc
    12901 tacgagctgc tggcagcctt tgccattgac cttatactgc tctacaccat tgttaagaaa
    12961 gattttgggt tctggggacc ttttggaata atcgccattg cggcaaccct ttgcctgttt
    13021 ttcaaccatc ccgttgaggt gaaaatttta atctgggtgg gtggtttgct aggcgtttac
    13081 ttcaacagga aattcatccc cctccgaacc aagcagttgc tcaataaggc gcccgaggat
    13141 gacaattagc taaaattggt acttgctgtg gtcagtcgca tggggtcatc agccggtagg
    13201 ctgatgaccc ctaatggttt acttcgttag gaacgagtag cgttcggagt actccagcat
    13261 ctgcctggca taatcctctg gataatcaac cctaacgtcc acaatgctgt cgttttgaac
    13321 tacaggcaca aggtctgggt tgataaatcc cccgtagggg gccaaattca acttggaata
    13381 gcgctccaga acttcgtggt gcagcgcggg gtcaactttt accccgaagc tctcaacaag
    13441 ttttttgccc gactcgtaat cgcccgaaga cttaatccgt tgaatttcac gcagaaggct
    13501 gccaaatagc tgccgtaact tctggaaatc gtttaccaca aagtaggttt tcccatttcg
    13561 gtttttcctc tcaacaacat tttcatcctt tccatactcg tagcaccatt gggcaatcag
    13621 ctggcggttg cgcatgtgtg cctgctcaat gtttttccca ggttccaccc gggttagctg
    13681 cgtcattaat ccgttgcgga tgtagttgct gtactgaacc ctggctacat caaggttggg
    13741 catgaggccc agctcaacca tttttggatc catgatatag taaagggcaa ataggtcagc
    13801 ccgagcctcc tctaatgggg agccgtagtt tttcagctca tcacctttaa ctcctggagc
    13861 cagctggcca gagccatgcc caaggcattc gtgcaaatcg gtgtgcaggc aatcggccag
    13921 gtaaccatac tgcttgttga gtgctacctc ctcgtccgac caggcaaact cctccatgaa
    13981 tccatttccc aacgaggctt ggttgtaggc gtaggtgata ttatccatgg taaccgattt
    14041 tgaaccatgc tgctttctta tccagtctgc atttggcagg tttatgccaa tgggggttga
    14101 cgggtagcaa tcgcccccga gctgggccac ggttatcacc ttggcgctta cccctttcac
    14161 ttccttcttt ttgaatatgc tgtcaatggg cgagtgatct tcaaaccact gtgcgttggc
    14221 gcttatcacc tcggtacgat gggttgcctc gtcgttcctg aagttcacca ccgactccca
    14281 gcttcccttg tagcccagcg gatcgccgta agtctcgatg aaaccgttga caaaatcaac
    14341 tttagactgt gtatcgctta gccagagtat gttgtactcg tcaaactttc tcaaatcgcc
    14401 tgttttgtag taatctacca gggtttcaat tattttttgc tgatattcat tttcagccac
    14461 tgtggcggcc ttctccagcc agaaaacgat tttaccgata gcctttccgt acatgccgtt
    14521 tgcataccaa acctgctcct ctatttttcc gttttttttc accagtttag agtttaaacc
    14581 gtaggatatg ggagtgctat cgttggggtt ggccagcctg gagtagaatt cttctgcttc
    14641 ggcctgtgta acaccctcgt agtagttgca ggctgaggtg gttaccaaat cagatccggc
    14701 aagctggtta actcgttttg gggctacgtc cgcatcgaaa attatgggtg taaaatgtgc
    14761 cagaatgcta tcgacgcccc caaatttctt ggggagcatc tcggacgggg tgttaccaac
    14821 cagctcggca aagtagcttc ggtcgaaatc gggcataatt ttatcggtgg agtagtggtg
    14881 gtgtatgccg ttggagaacc aaacccgctt caggtaaacc ataaagctac cccatcgtgg
    14941 gtcgttgcgg tttcccttgt aaccctggta aactccctcc agaactgagc gcacatcgag
    15001 gttgaacttg tagttttggt cccaaatgat atccctaccg catttagctg cttcactaag
    15061 gtagtacacc agcacccttt gtttaggtgt gagcgaatcg aagcccggaa tttggtagcg
    15121 catgatgcgg aggtcggcaa actggtcaac ttgccactta aaaggttcgg cctgctcgtt
    15181 tcccgattta catccaccag tcgagaaccc ggtagcggcc attaacaata gtgccattat
    15241 ttttctgttc atttttagtt gagttgcaaa atgtatgagg tgtgtatgct attttctctt
    15301 ttgctggggt ttatatagaa atgagggttc gtctatcttt tctggtgcgt caacaggatt
    15361 tactttttct ctggaggggt tacgcatgtc caggtcccta acataaaccc attttccatt
    15421 ttcaaaggca aaaccatcta ttgaggcatc aggcccgtag aactgaaaat caccggtaaa
    15481 ttcgggttgg gagggcgaca ggtgatcgaa tgcaattatt ttttgctcat catagtactt
    15541 gaggttgaaa ccggctcttg cgctaaactc aaaaacaatt ctcgaaagca gctgcttgtt
    15601 atccacgtta aaaactggca ggccaaaaac aggttctccc tgggggctga gggtgagcac
    15661 ctcgattacc ttttttgagg tgaagaggtt gttgtagtcg aatcctagca acacgtactg
    15721 cttttctgag ccgtagggta gttctgccag ctggtagtac agagctccaa gccacctgtc
    15781 ggccggtaac ctttcgtgtt gcgggtcctt taccatttcc cggctatccg tgagggcata
    15841 aagtcgggat ttatctccat ccagtcgaac cagaacaaac ccgaagtagc gatggtagcc
    15901 acctgtttgg ggaatattcc aggtgattat ccttaccttt tgatcactgg aagtgagcat
    15961 tcccacgtgt tttagtgagc tgaatgggta atctattgcc ccatcatgct caagagtgtt
    16021 tttaaagttc tcatggaaaa ttctattcag cgagtcgcgc cgggtggtgg atgtttcgct
    16081 tacgatgagt gagagcagct tagaaagcga gtcctcgcgg gtagaaaaga agttggcatt
    16141 aacttcttgt ccaaacccca caaggggtag aataaccagg gcagccgcaa cgttaattag
    16201 gattactttg cgcataagct agcggtttat ttcgtttaga ccttggtaga ttaggttggc
    16261 cacctcttcg cattgctcct ttgttatgca aaggggaggt gcaagcctga tggccgtgtt
    16321 gcaaaataga aaccagtcca gcacgaaacc atgttccact ccatatttta ttaaggcgct
    16381 gaccttctca aacgaaccta gctctatggc catcagcaga ccgaatgacc gaaactcttt
    16441 aaccagcggg tgtttcgata ggatatccct gaatatttgc tccttttccg gaacgctttc
    16501 ggggagcctg tcgccggtta aaacgtcaat tgcggcgagc gatgcggcac aggaaaccgg
    16561 atgacctcca aaggttgtaa tatgcccaag ggaagggttt tccatgaggc agttcattat
    16621 tttctgtgag gaaatgaatg cccctagggg cataccacca cccagcgcct ttgctaggag
    16681 caggatgtcg ggtgtaaccc caaatcgctc tagcgcaaac atctttccgg ttcggcccat
    16741 gcctgtttga atctcgtcaa aaatcagtaa agcgcccacc tcggagcaac gacgacgtag
    16801 ttcggtaata aaattgccat cggcaaccct tatgcccgcc tcgccctgaa cgggctcaat
    16861 tatcacacag gcagtgcggt tggttattcg ggcaaggtct gctatactgt tgaattcaag
    16921 tatcgaaata tccgggagta atggccgaaa ggatcttttc tggctttcaa cccccatcac
    16981 gctaagcgca ccctgtgtgc cgccatggta ggcatttcgg aacgagatta tttctgtcct
    17041 accagtaaac cttttggata gcttcattgc cccctcaaca gcttcgcttc cagagttaac
    17101 aaagtaaacc gagctaagcg tctggggtag catggatgaa agtttagcgg ccagtagtac
    17161 ctgagggctc tggatgaact cgccgtaaac catcaggtgc atgtatgccg aggcctgatc
    17221 ctgaacagct ttgactactc tggggttagc gtgtccaaca ttgctcaccg agattcccga
    17281 aatcaaatcg tagtattttt cgccacttgg cccaaaaaga aaaaccccct cggctctgct
    17341 tacctcaagc atcaggggtg catcggatgt ttgggcaaca tggctaagaa aaagttgcct
    17401 gatgttctgc atataaaagg ttacctgcta ttacttgttg atcagattaa tttcgcttaa
    17461 cagtttatcg cggtatgact ttccgatagg gattatttta cgtccctcag gagttcggat
    17521 cactgtagcg ttatcctcaa tgctttcaat tttgccaaca ttcactataa atgagcgatg
    17581 aatcctttta aatcgaaccg aagaaagttg attttcaatg gatttcatgg ttagatgaat
    17641 ggtaaaccgc tcgttaacgg tattaataac aatgtagttt tcaagtgcct ctatccatag
    17701 tatgtcgcta tactttaccc gaatcaacat gttgtttttc ttgatgaaga tctccttgtc
    17761 atcgtcgtag ggagagcgtt gctggttaaa cttgtcgtaa gccttgctaa cagcttttgt
    17821 aaagcgggga aatgtaatgg gtttcaggag gtagtcggta acgctgtagt caaatgcctt
    17881 aagggcgtac ttttccttcg aggagattat aataacctgt ggttcttggg aggtagcctc
    17941 taaaaagtct atgccgctca tttcgggcat ctctatgtcc agaaaaatta aattgactgg
    18001 ttcaagtgaa tttttaagaa aattaagggc atcaactgcg ttgggaaaag agcccgttag
    18061 ccgtatgaag tcaatttttg aagcataatc ttcaataact ctgcgcgata gctcatcatc
    18121 atctacctgt atgcagttca tcttgttcta gttaagttaa cctgaaaaat tctttctaaa
    18181 gaacctacac aaacttaatg caatttatta tactctgcaa caaatctttt ttcgacaatg
    18241 tggcagtttt tcacggattt tcttaaccag tcgagtagca caacctcttt ctcatattcc
    18301 gacagggggt atgcggggtt tgcttttgcc cggtgaacca attcgtagag ggttatggca
    18361 acagaaaccg atatgttgta gctttcggtg aagccgtaca tcggaatggt gataaattct
    18421 tccgaaaggc tcatggcctc ttcggacaat cccaagagtt ctgtgccaaa aagaatggca
    18481 aattttcctt tttcggtatc gaaatcgcga atgctgcacc ccagtgaatg gggtgaggtg
    18541 gcaactatcc ggtagccgct actcctcaat gtttgtaacg cctcttgggt tatgctctta
    18601 ttgccggaat actttttaat ggtcagccat tttgatgcgc ccatgtcaac ctcggggtta
    18661 acgttaaaag aattcctgtt ttcgatgata tgaacatcct gaatgccaag gcattcacaa
    18721 cttcttagag ctgcgctggc gttttgaggt tggtacaaat cctctagaac cacagtaatg
    18781 tagcgtgttc gtctgctcaa aacctccctg agcaagttta gcctgcgttc aagcaccagg
    18841 tcagacaggt agctggttag ctggagacga aactgccgat cactctgtat taaatggtca
    18901 atgccgctca ttccatacta ttatcattct tactaagtgg gttatgcagt tcagctcctg
    18961 gggtgattag gtcttgtaac agctagcatc atccgactga atagggttaa cgcccttcaa
    19021 ccaggttagc tgatgggtaa aatttacccc ttaagccgaa tggctaccat gattaatttg
    19081 cctggctgga agcataacat gtaacaaaag gctagcttgc acggctttac cttcaagtaa
    19141 aaaaaaagga gaacactaag gttctccctt gggattttct gtattagcta ttgaactttt
    19201 ccattcagtt cgctaccagc tttaaactta acaaccttct tagccttgat gttgatgggc
    19261 tttccggttt gagggttacg tcctgtgcgg gcgctcctct tggcaacgga gaatgagcca
    19321 aaacctacca gggcaactct atcacctttc ttaagggcgg tgccagtagt tttgatgaaa
    19381 gcatcaagtg ctttctttgc atcagccttg gtaaccttag cttcagctgc aattgcatca
    19441 attaattgag ctttgttcat aatagataaa ttttagggtt aaaaaatagt tgttagttgg
    19501 ctttccatga caaaattatt acataaatca gtaaaagcaa gttttttcaa aaaaaaattt
    19561 gtcatttcag ccttttttgt tggtttttaa gaaaccaaaa tttttaccaa cgctaaatta
    19621 ttgaattttt aaacaatcat gcaactattt ttataagaaa attcgttttt ttttgatttt
    19681 ctccgcaaaa tttctaagtg aaaaacgaca ttaagtgtta taatgtatta tatatcaata
    19741 acatacaatt tatttcaggt atctacttga ttttaaaaaa attatcacca agttatcaac
    19801 agttgctatg ttgtaggctg tttttaccac tattgcctca aagttttacc ctaaatatgc
    19861 ctggaattat atactttttc ccttttttgc tttgcaggaa taaaaaaagg cggtactttt
    19921 gccgccggag aaatggcaga gcggtcgaat gcggcggtct tgaaaaccgt tgttcgcctt
    19981 acggcgaacc gggggttcga atccctcttt ctccgcagaa ggcaag
//
LOCUS       DAOPHX010000034        19660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_2993_length_19660_cov_5.938281, whole genome shotgun sequence.
ACCESSION   DAOPHX010000034 DAOPHX010000000
VERSION     DAOPHX010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 19660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19660
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2993_length_19660_cov_5.938281"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PK502_04065"
     CDS             <1..196
                     /locus_tag="PK502_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00026.1"
                     /translation="REYVEPMSLAAVMAGADGLLLEVHQTPERALSDGQQTLNFEESH
                     RLFLKVQKLVALRERLEVAD"
     gene            complement(193..843)
                     /gene="rpe"
                     /locus_tag="PK502_04070"
     CDS             complement(193..843)
                     /gene="rpe"
                     /locus_tag="PK502_04070"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPH00027.1"
                     /translation="MTHLVAPSLLSADFANLSADIEMVNNSLADWFHLDIMDGRFVPN
                     ISFGFPVVERIRDKASKPLDVHLMIVDPDRYLERFHDAGASWLTVHYEACTHLNRTLD
                     QIRKLGMKAGVALNPHTPVELLIDILDYTDLVLIMSVNPGFGGQKFIDSSLGRIERLR
                     AMIDKRRLSTLIEVDGGVDTNNASALYRAGANVLVAGNAVFKANKPLDVIAQLRKA"
     gene            complement(943..1806)
                     /locus_tag="PK502_04075"
     CDS             complement(943..1806)
                     /locus_tag="PK502_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPH00028.1"
                     /translation="MRQLKIIKQVTNRETVSLDKYLHEIGKVDLLSTDDEVALARKIK
                     EGDPEALSKLVKANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQARIIKLPLNKIGSINKVNRALVELEQKFEREPTINELSE
                     VLMLAPEDIKEALRSSGRPISMDAPLTQDEDSSMYDVILSPDSPQPDRGLLNESLRRE
                     IERALSTLTPRESSIIRMYFGLNGKQPLTLEEIGEELDLTRERVRQIKEKALRRLKQT
                     TRSKILKSYLG"
     gene            2290..4149
                     /gene="rho"
                     /locus_tag="PK502_04080"
     CDS             2290..4149
                     /gene="rho"
                     /locus_tag="PK502_04080"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPH00029.1"
                     /translation="MYDILELNKKLLPELREIAKQLNIKKLESLKKQDLIYKILDQQA
                     INASMPGKGKEKERPSTSRPAVKLQPTEVVNKEQNAEAPLQQERSDSRDSAGKRVRFT
                     RNKSAEVKKEYSSTPARGEKVSKFSKQPDANELSIRTEQSNFPSSKEVFLPEVDLEPQ
                     TQREKKEDEVFADVLADDTQGIPEAEVSQSDSTPNIKIEEENFPLNPQESAVSNQLPE
                     PAGDGKHHPREHSGHKNVEKRNNERFDFDGIINSTGVLEIMPDGYGFLRSSDYNYLSS
                     PDDIYVSQSQIKLFGLKTGDTVTGTVRPPKEGEKYFPLIKVMEINGRNPEFIRDRVPF
                     DYLTPLFPNEKFTLIGNGMNLLSARIVDMFAPIGKGQRGLIVAQPKTGKTILLKDIAN
                     SIAANHPEVYMIVLLIDERPEEVTDMARSVNAEVISSTFDEPAERHVRVANIVLEKAK
                     RLVECGHDVVILLDSITRLARAFNTVQPASGKVLSGGVDANALQKPKRFFGAARNIEN
                     GGSLTILATALTDTGSKMDDVIFEEFKGTGNMELQLDRKLSNKRIFPSVDITASSTRR
                     EDLLLSKEVLQKIWILRNYLTDMNSVEAMEFLRDRLVKTATNEEFLWSMNSDI"
     gene            4232..4632
                     /gene="ssrA"
                     /locus_tag="PK502_04085"
     tmRNA           4232..4632
                     /gene="ssrA"
                     /locus_tag="PK502_04085"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(4762..5376)
                     /locus_tag="PK502_04090"
     CDS             complement(4762..5376)
                     /locus_tag="PK502_04090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPH00030.1"
                     /translation="MLRIAELICLFVLVPLAFYFDFIPLPKFVPLLVVFIYALVFLLR
                     SKTFSRKEFGLNGYRSWPGLILRVVAVTIVIVVFSYYLLPDKFLIMPREQPLMWAMIM
                     IFYPVWSAFTQEVIYRTYFFHRYGAAIPNEKVLALVNGLLFGFLHIIFRNWVAVIGAT
                     LMGLLWAFSYLKHRSLLVVSIEHSIVGDMLYTFGLGYFFYVPDF"
     gene            complement(5581..6798)
                     /locus_tag="PK502_04095"
     CDS             complement(5581..6798)
                     /locus_tag="PK502_04095"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPH00031.1"
                     /translation="MVQRYKIGIVRETKTPPDRRVALPPNQARELTTRYSALEIAIQP
                     SSIRCFSDEEYARIGIKLQEDLSDCDLLIGIKETKIPTIIPGKTYMEFAHVAKKQPHN
                     RPLIQAFADQGNTLIDYEYLTDDKGNRLIAFGHWAGIVGAYNAIRALYIKEDLPELKP
                     AHQMHDYKEMQEVLKGLKVIPKKFVITGGGRVAGGAMEVLAQAGVKEVTPDEFLKGQF
                     DYPVYCRLDPWHYAKRKDGKPFDWSYWLSNPADHDTAFIPFTSATDILIACHYWDYRS
                     PHFFSREDMLNNSFRISIIADVSCDIPGPIPSTIKASTIADPFFDYNPKTDMEEPPFS
                     SKGNVTVMSIDNLPGELPRDASEFFGNILIEKIIPHIVTGDKEGIIERATILKNGKLT
                     GKFSYLSDYLKGE"
     gene            7107..10634
                     /gene="nifJ"
                     /locus_tag="PK502_04100"
     CDS             7107..10634
                     /gene="nifJ"
                     /locus_tag="PK502_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPH00032.1"
                     /translation="MAKSKKFITCDGNYAAAHIAYMFSEVAAIYPITPSSTMAEYVDE
                     WAAFGRKNIFGDTVRVVEMQSEAGAAGAVHGSLQSGALTSTFTASQGLLLMIPNMYKI
                     SGELLPGVFHVSARSIAAHALSIFGDHSDVMSTRQCGFAMLATSSVQEVMDLAGVAHL
                     SAIKSRIPFLHFFDGFRTSHEIQKIEVLENDDLAPLLDQKSLQEFRDRALNPEHPVTR
                     GTAQNPDIYFQAREASNKFYMDVPDIVEGYMQEIAKLTGREYHPFSFYGDPNAENIII
                     AMGSVTDTIRETIDYLMAKGEKVGMISVHLYRPFSAKYFFKVLPSAVKRIAVLDRTKE
                     PGANGDPLYLDVKELFYGREKAPIVVGGRYGLSSKDTTPAQIIAVFDNLKLSEPKNQF
                     TIGIIDDVTFKSLPTGKEVNLAPKGTFQAKFFGLGSDGTVGANKNSIKIIGDNTPKYC
                     QAYFAYDSKKSGGITVSHLRFGDQPIRSPYLVITPDFVACHVQSYLGKYDVLKGLQPG
                     GTFLLNTIWSKDELNEKLPNSIKRYMAENSIKFYIINATQIAEEIGLGNRTNTIMQSA
                     FFKISGVIPYDVAVKEMKKAIEKSYGRKGEEVLRMNFAAVDRGGDAIQVVVPMEWAKL
                     QPENKLEIEGAPEFINKVVLPINLQMGDDLPVSAFVGREDGTFPAGTTRYEKRGIAVN
                     VPEWISENCTQCNQCAYVCPHAAIRPFLLTEEELKNAPAGTKTVRGNGGIKDFNFRVQ
                     VSVLDCTGCGNCVEVCPSKEKSLVMRPIESQMAEAARFDYMHEKVGYKDILGKEKSVK
                     NSQFAQPLFEFSGACAGCGETPYIKAITQLYGDRMIVANATGCSSIYGGSAPSTPYSA
                     GPDGRGVAWSNSLFEDNAEYGFGIATGVAKLRQRIALKLQEALEGNFTSTTKEAMKEW
                     IAGKDNAFLSKAASAKLIAELSKESNPLAKELLSLKQYFEKKSIWIFGGDGWAYDIGY
                     GGLDHVLSTGEDVNVLVLDTEVYSNTGGQASKSTPAAAVAKFAASGKRVRKKDLGMMA
                     MSYGYVYVAQVAMGADHNQYLKAIKEAEAYPGPSLIIAYSPCINHGIRKGMGSTQSEA
                     KQAVKSGYWHLYRYNPLLEAQGQNPFQLDSKEPDWGMFQDFLKGEVRYTSLQLSFPDQ
                     ANELFKAAEENAKWRYNSYKKLAGVVEKP"
     gene            10893..13925
                     /locus_tag="PK502_04105"
     CDS             10893..13925
                     /locus_tag="PK502_04105"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPH00033.1"
                     /translation="MLSKIKNYLAKLHIPYFPFARYLKAGGSAIGFMLIGISGGHSQI
                     VSQPVTRHITVDEGLAASEVYHVFQDSRGYIWFGTNNGVSRFNGYEFENFDLVSGLPD
                     NTVFEIYEDYKHRIWFIPFSGSLSYYENGEIKSYRYNSKIVEHLPNSRGPSKLSFFVD
                     SLDNVSFGLKDFGIISIDQEGIYKHIDDSNTDAQVVVRSVGSGKFLVSDSHEPRKYGL
                     FYFDGRQSYRFTLSELGIKPFKPIYFYAQQAKDGSAFITIDGNIFRIKDGRVVSHNHS
                     ITQSVIWISIDADNNLWVSAFEGGVYMFPNCDLESPERLCFLKDYQVTSVLRDDEGSY
                     WFSTLNDGVFYVPDHQIAVFSKENGLSDNRANAVYAKDGKIYVGFEMGFVDVIENGQI
                     THYKLSSKDNIGITYVRDIYGDTLTGRVLVAAFGHVAYFENNKLKKLIPDSFNLNTRR
                     IIGSRGGGYWLATAKGFYKIENNRIAYDSRSDGFNGMVFSMAEDYKGDLWFNTVNGLW
                     RYSDSNLLFMGSENQLLAKIGHSMFFSHRDSSLWIGTNGAGIVIYGKDGKVSQISAAD
                     GLVSNSIHYLFPEGNRIWAATRQGLSILTLVNDTTYNIQNLTVLDGLPSNEVTTVFVM
                     DGIAYIGTNKGLAILNYSNLGFNHTPPKIEVLGVFVDGRPVAPVNGAVQINYSSNVVN
                     FSYIGLAYRNMGNILYRYRLMGLDSTWVFTRSTSCIFSNLKPGSYTFQVQAQNSNGIW
                     SSRSASIDLIIRPPFWERFWFIILAATILAGTVYAVYSLRISEIKRRNELINSINLYK
                     QQSLRQQMNPHFIFNTLNSIQYFILERDTVSSHKYLTKFARLMRLTLDNSLSPTVSLR
                     DELEALRLYLDLEALRLEGKFTYSIDYNDDESILNNKIPTLMIQPFVENAIWHGIMLK
                     KEKSGHVWVKIADEGSRIVCTIRDDGVGRTKAQRIQEQTGKEYSSRGFQITKQRIELL
                     NTLYGEKFSIKFIDLFDDMGNASGTLVSISIPKNLDVV"
     gene            14114..14878
                     /locus_tag="PK502_04110"
     CDS             14114..14878
                     /locus_tag="PK502_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPH00034.1"
                     /translation="MLKALIVDDEIASFRTLEVLVGQFISNVQIVDIARSAAEALDKV
                     ERISPDLVFLDIEMPGGDGFDFLERCAARKFEVIFITAYNRYAIRAFRYSAIDYLLKP
                     IDVDELRAAVERAIKRRYANFDGHNKYYALFENLRSVIPSKLVVTANQVCEYINLNDV
                     CFLEDDDKMTRITFTGGNTMRVENQLSYFEEILGDRHFYRINSKQLVNVDHIRRIANR
                     ASDNLELDNGTLLAVDVGLKAGLERCLEKYIKLGKE"
     gene            14878..15300
                     /gene="ruvX"
                     /locus_tag="PK502_04115"
     CDS             14878..15300
                     /gene="ruvX"
                     /locus_tag="PK502_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPH00035.1"
                     /translation="MGRILALDIGQKRTGIAVTDPLKIIANGLTTIPSHTVLGFLADY
                     FSKESVELLVVGSPKQMDNSSSEAMKFIDPIVRGIRKTFPGMPIQFIDERFTSKMAKQ
                     AMIDGGMKKMDRRNKAMVDTISATIILQSYLESLSFNR"
     gene            15369..15923
                     /gene="def"
                     /locus_tag="PK502_04120"
     CDS             15369..15923
                     /gene="def"
                     /locus_tag="PK502_04120"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPH00036.1"
                     /translation="MILPIYVYGSSILRKVAQDINPDYPNLKRLIDDMFETMSQSDGI
                     GLAAPQVGLSIRLIVIDGTPLAKDDPSLKDFRKVIINPYIEERFGEKVIYSEGCLSIP
                     NIHEEVEREEKVRISYLDENFTAHNEVFEGFAARIVQHEYDHLEGILFTDRLSPIRKQ
                     LLRSKLSAISKGKFTADYKCRLVK"
     gene            16201..16776
                     /locus_tag="PK502_04125"
     CDS             16201..16776
                     /locus_tag="PK502_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00037.1"
                     /translation="MDKKLVFKDVLSNGVSTGLKNFLSLIGCVVLWLLTVWIPYINVG
                     TTIAIITLPAALSKGAVISPLEIFNKRYFRYMGEFFLVEGLAGIIIFSAMLFMFFPAI
                     VMSIAYSFGALLVVDKGKGASEALRLSNKATYGNKWTIFWVQLVLILPMFIFAWIWRP
                     LAIIYAILYVPFTLGATAFIYKELTGDISEE"
     gene            complement(16871..18748)
                     /locus_tag="PK502_04130"
     CDS             complement(16871..18748)
                     /locus_tag="PK502_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HPH00038.1"
                     /translation="MKKLLALFFLVSLIVGNAYTQELPEKNLKIQVEEVTVFIDGAQL
                     TGKATTELSKGKSLLKFTNLSPYIDAKSIRVKASNSVSVLAVNHQQNFVDRLQKPKEL
                     TDLEATLRTVAEGLKLENINLEINSEELKFMQDNRIIGGKNQETSVANFRETSSFYGT
                     KISALKLKELEINKKIEELNKKQNELQNQLNTLTSQKQYPSGEVMVNVEASNDVTATF
                     EVTFMVGNAGWFPSYDIRALSIDQPVVLVYKANIKQDSKTDWKNVKLKLSTSEPNVSG
                     VPPTLKTYFLNYNTLPPTYSQQLGRVTGRVMDSERNPLPGVSVTVTGTTISTVTAADG
                     SYYITLPASASYLTFSFVGYQSQTLPVNPIMNVVLSENNMMLDEEVVVTAYGITAEKR
                     KLAAPSSKSADEATYMDEAGGVVGQQGIAQKPTAVDFEIREPYTVDSDNKNHTVEMVT
                     YSLPAMYEYYSVPKIDRSAFLLARITDWEKYSLLDGEANIFFENTFVGATVLDISRAT
                     DTLQVSLGRDKGISINREKIKDYTTRQLVGTKKEETRTWRTTVKNNKLQKISITVMDQ
                     VPVSTSEEIEVNILETSGGKVNTENGEVTWELTLEPGAEKKLDLKYSVKYPKNKVLVI
                     E"
     gene            19222..>19660
                     /locus_tag="PK502_04135"
     CDS             19222..>19660
                     /locus_tag="PK502_04135"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPH00039.1"
                     /translation="MKRIDFTVEWHNSVTSSNSICAQRCEEGAAEGLVIAAMFQEMGR
                     GQRGNSWESEAGRNLTFSILIRPGHLRVDEQFYLSKVAAASVCDWVSAYVSGDDVTIK
                     WPNDIYIGDSKVAGILIENSFSSAMLDTSVVGIGINLNQQTFPD"
BASE COUNT         5337 a         4285 c         4527 g         5511 t
ORIGIN      
        1 tagggaatat gtagagccta tgtcgttggc agcagtgatg gcaggggcgg atgggctact
       61 acttgaagtt catcaaacac ccgaaagagc gctatccgat gggcagcaaa cactgaattt
      121 tgaagaaagc caccgtctct tcttaaaagt tcaaaagctt gtagcccttc gggaaaggtt
      181 ggaggtggcg gactaagcct tcctgagctg agcaattaca tccaggggtt tattggcttt
      241 aaacacagca ttcccggcca ccagcacgtt ggctccggct ctgtatagag ccgatgcatt
      301 gttagtatca acgcctccat caacctcaat cagggtgctt agcctccgct tatctatcat
      361 tgctctcagt cgctcaatcc tgcctaggga gctatctatg aacttttgtc caccgaaccc
      421 cggatttacc gacattatca gcaccaaatc ggtgtaatcc aggatgtcta tcagcagctc
      481 aacaggagtg tgggggttga gagcaactcc ggccttcatg cccaattttc gaatctggtc
      541 aagggtccgg tttaaatgag tgcaagcctc gtagtgtacg gtaagccagc tggcaccggc
      601 atcatggaat cgctccaggt acctgtcggg gtcaacaatc atcaggtgga catccagggg
      661 tttactggct ttatccctga ttcgctctac taccggaaat ccaaatgata tatttggcac
      721 aaacctgcca tccatgatat ccaggtgaaa ccaatctgcc aggctattgt taaccatttc
      781 aatatcagca gaaagatttg caaaatctgc cgagagcagc gatggagcaa caagatgggt
      841 cataagcaat cggtttttgc aaaggtagca atttaaaata ctaggtgttg ccccgaatgc
      901 tttaaggcat gttggtgaga aaaacggaag ggctaagcgg ggctaaccca ggtatgactt
      961 gagaattttg ctacgagtgg tttgcttgag ccttcgaagc gctttctcct taatctggcg
     1021 tacccgttcg cgggtaaggt cgagctcctc gccaatttcc tcgagggtta agggttgctt
     1081 tccgtttaga ccgaagtaca ttcttatgat actggactca cgaggcgtga gggttgatag
     1141 ggcacgttct atttctcgtc ttaaactttc attaagtaat cccctgtcgg gttggggaga
     1201 atcgggcgaa aggataacat cgtacatgct actatcttca tcctgcgtca gtggagcatc
     1261 catggaaata ggacggccgc tgctccgcag ggcctccttg atatcctcgg gggcaagcat
     1321 tagcacctcg gacagctcat taatggtggg ttcccgttca aacttttgct ccagctcaac
     1381 cagcgcccta ttcaccttat taattgagcc aattttgttc aaaggtagct ttataattct
     1441 tgcctgctcg gccagcgcct gcagaataga ctgcctaatc caccaaacgg cgtaggatat
     1501 aaatttaaat cccctcgttt cgtcaaagcg ctgagctgct ttaataagtc ccagattacc
     1561 ctcgtttatc aggtcgggta ggcttaaacc ctggttctgg tactgcttgg caaccgatac
     1621 tacaaatctg aggttggctt ttaccagctt ggataacgct tcaggatcac cctctttaat
     1681 ttttcgggca agtgccacct catcgtctgt actaagcaaa tccaccttgc caatttcgtg
     1741 cagatactta tcgagcgata ccgtttcacg gttggtaacc tgcttgatta tttttagctg
     1801 cctcattcca ataacgatgc tttgtttggg ggtgaatata attacaatta ttaaattttg
     1861 ttaatgcttc gattttttta tgtaattttt tttaaacctt ttggtggcgg tttccatcta
     1921 atgccatttt tttaatagtc aatgccatgt tctataatgg tttttatttt atattcaaca
     1981 aaaaatcatc ataaaattct tgcaggtgaa aaataatatt tcataccttt gcaaaaaata
     2041 accgggaaga gaactccaac cctctttctt caatttcact tcaacaacta tttcatcact
     2101 gagtaatttc atctaaagtt atttacctga cagctttaca atatgttaac gtatttatat
     2161 taagcattac actaggatgt aagcaaaagg cttgcccatt gattaccatt ttacaagcct
     2221 atgcaaagta ttttttccaa tttttcttta actactctaa aaccttcatt ttacaactaa
     2281 actattttta tgtacgatat tctcgaactc aacaagaagc tccttcccga actgcgggaa
     2341 attgccaagc agctaaatat caaaaagctt gaaagcctga aaaagcaaga cttgatttat
     2401 aaaattttgg atcagcaggc tatcaatgct tcaatgccag ggaagggcaa ggaaaaggag
     2461 cgcccaagca cttcccgacc ggctgtaaag ctccagccta ccgaagtagt gaacaaggag
     2521 cagaatgctg aagccccact acagcaggaa agatcagaca gcagggatag cgcggggaaa
     2581 cgagtgcggt ttacccgaaa caaaagcgca gaagtaaaaa aagagtactc ttctactccg
     2641 gcaagaggag agaaggtgtc caaattttca aaacagcctg atgcgaatga attatctata
     2701 agaaccgagc aatccaattt tccctcaagt aaagaagttt ttcttcctga agttgacctg
     2761 gagcctcaaa cccaaagaga aaaaaaggaa gatgaggttt ttgccgatgt acttgctgac
     2821 gacacgcaag gaatacctga agctgaagtg agccaatccg acagcactcc taatatcaag
     2881 atcgaggaag aaaattttcc tcttaacccg caggaatcgg ctgttagcaa ccagctaccc
     2941 gaacctgccg gtgatggcaa gcatcacccc cgtgagcatt cggggcataa gaatgttgaa
     3001 aaaagaaaca atgaaagatt cgactttgat ggtataatca acagtacagg ggtgcttgaa
     3061 ataatgcccg atgggtatgg gtttcttcgc tcatcggact acaactatct tagctctccc
     3121 gacgacattt acgtttcaca gtcgcaaatt aaactgtttg ggctcaagac tggcgatacg
     3181 gtaacgggta ccgttcgtcc tccaaaagag ggcgaaaagt actttcccct tatcaaggtg
     3241 atggaaatca acgggcgtaa ccccgaattc atacgcgaca gggtaccctt cgactaccta
     3301 accccgctct ttcccaacga gaagtttacg cttataggaa acgggatgaa cttgctttcg
     3361 gcgcgtatag ttgacatgtt tgcccctatt ggcaagggtc agcgggggct tattgttgcc
     3421 cagcccaaaa ccggtaaaac tatcctgttg aaggatattg ccaactccat tgccgccaac
     3481 catccggagg tttacatgat tgtgctgctg attgacgaac gacccgagga ggtaaccgat
     3541 atggcgcgta gcgtcaacgc cgaggtaatt tcatcaacct tcgacgagcc tgccgaacgg
     3601 cacgtaaggg tggccaacat agttcttgaa aaggctaaaa ggctagtgga gtgcggtcac
     3661 gatgtagtaa tcctgctcga ctcgattacc cgcctggcac gcgcgttcaa caccgttcag
     3721 cccgcatcgg gcaaggtgct ctcgggtggt gttgatgcca atgccctgca gaaaccaaag
     3781 cgtttcttcg gcgcagctcg aaacattgaa aacggcggtt cgcttaccat tttggccact
     3841 gctcttaccg ataccggcag caagatggat gatgtaattt ttgaggagtt taagggaacc
     3901 ggcaacatgg agctccagct cgaccgcaag ctatccaaca agcgaatatt cccctcggtt
     3961 gacattacgg catccagcac ccgccgcgaa gacctgctgt taagtaagga ggttctccaa
     4021 aaaatttgga ttcttcgcaa ctacctcacc gacatgaact cggtggaagc catggagttt
     4081 ctcagggaca ggctggttaa aacagccacc aacgaggagt tcctctggtc gatgaatagc
     4141 gatatatagc tttatttttg caaactttaa ccgccgaatg ttgatatccg gcggtttttt
     4201 attgtatctt tgccgttgct aaaaccccat tggggctgac gtggttttga cagcaagtag
     4261 aatgggtttg taagcatgcc gagcgtcgtg atgctggctc gataatcaca ggtcacaacc
     4321 aaataaacgg cgaatcaaat tacgctctcg ctgcttaatt ttgcaaagca aaatggctta
     4381 atccctacgc ctaggtgtgt ggggacgaga tgtcccgccg gaaattccgc ccgattgttt
     4441 ccggcatacg gggcaccgac aaaaccggga tagctcaagc gacaccttta agcttgggta
     4501 aaataataaa ggttaaggat tggttcggtt gcctttcgcc tggctagtcc cgacaaccaa
     4561 gaaaggctaa gcatgtagaa aacaagctgg ttccttgttt ggacgagggt tcgattccct
     4621 ccagctccac cagcatgccc gtatgatggc ccaaagcgct caaatgttat aatttggagc
     4681 gcttttttta tttcaagaat atacccttac tccagaagtc tttgttatac tttccatttt
     4741 ttttattttg gagagaggct ttcagaaatc gggaacatag aagaaatatc caagtccgaa
     4801 ggtgtagagc atatcgccaa ctattgaatg ctcaatagaa acaacaagta acgagcgatg
     4861 cttcaggtag ctgaaagccc acagcagccc cataagcgta gctccgatta ctgccaccca
     4921 gtttcggaaa atgatatgaa gaaaaccgaa tagcaaccca ttcaccaacg ccagaacttt
     4981 ttcatttggt atagctgccc cgtaccggtg aaaaaagtat gttctgtaaa tcacctcctg
     5041 ggtaaaagca gaccaaacag ggtaaaaaat cataatcata gcccacatta gaggctgctc
     5101 acgcggcatt atgaggaatt tgtcgggcaa caggtagtag ctgaatacaa cgattacaat
     5161 ggttactgcc acaacacgca gtatcaaccc aggccagcta cggtacccat taagcccaaa
     5221 ctcctttcgg ctgaaggttt tagacctaag caggaatact agagcataaa taaagactac
     5281 cagcagggga acaaattttg gcagtgggat aaaatcgaag tagaatgcca gtggaaccag
     5341 cacaaacaaa catataagct cagcaatacg aagcatagga aaaactatcc aacttgagga
     5401 ttaaatttaa cagctcagcc tgccaaaagt ttattttagg cttagtgttg ttcaattatg
     5461 ctagtttagc tctaagtaac accccacacc gaactaccat tccttggccc aaaagcccga
     5521 atatgccctg tcttaataaa gataaactgt ccaacagctt tgagtaccct tttttagagg
     5581 ttattctcct ttcaggtaat cggataggta ggaaaatttt ccggttagtt ttccattttt
     5641 aagaatggta gcacgttcta ttattccttc cttgtcaccg gtaacgatat gtggaataat
     5701 tttttctatc aggatattcc cgaaaaattc gctggcatcc ctgggtagct ctccgggaag
     5761 gttgtcgatc gacataaccg ttacattgcc tttggacgag aagggaggct cctccatgtc
     5821 ggttttaggg ttgtaatcaa aaaagggatc ggcaatcgtt gaggctttaa tggttgatgg
     5881 gatcggcccg ggaatatcgc agcttacatc ggcgataatc gaaatgcgaa agctattgtt
     5941 tagcatatcc tcacgcgaaa aaaaatgggg agaacggtag tcccagtagt ggcaggcaat
     6001 caatatgtcg gttgcacttg taaagggtat aaaagccgtg tcatggtcgg ccgggttact
     6061 caaccagtag ctccagtcga aaggctttcc atccttacgt tttgcatagt gccatgggtc
     6121 taaccgacag taaacagggt agtcgaactg acccttcaga aattcatcgg gggtaacttc
     6181 ttttaccccg gcttgtgcca gtacctccat agcgccgcca gcaactcgcc ccccgccggt
     6241 tatgacaaat tttttgggga tgaccttcaa ccccttcagc acttcttgca tctccttgta
     6301 gtcgtgcatc tggtgggctg gctttagttc cgggagatct tctttaatgt agagcgctct
     6361 aatggcgtta taggctccaa caataccggc ccagtgccca aacgcaataa gcctgtttcc
     6421 cttatcatcg gttaaatact catagtcaat tagtgtattg ccctgatcgg caaacgcttg
     6481 gattaacggg cggttatggg gctgcttttt ggctacatgg gcaaactcca tgtaggtttt
     6541 acccggaata atggtgggta tttttgtctc ttttatccca atcagcaggt cgcagtccga
     6601 taaatcttcc tgaagtttta tgcctatcct ggcatactcc tcatcggaga agcaccttat
     6661 gctacttggt tggatagcaa tttcaagggc tgagtacctg gtggtaagct ccctggcctg
     6721 atttggtggc agcgctactc ttctgtcagg tggcgttttg gtttctctta caatacctat
     6781 tttatatcgt tgaaccatag catttgattt aaggtgaaaa aatgttaaaa aaaatcatga
     6841 acctgcgaat ctaacaaact gtaattaaat ttccaataat agcttactaa attctgaagt
     6901 atttctcgtt ctgtaaacaa gtgtaaggta atcgtgctat tcgattttaa attttacttt
     6961 atatgaaacg gagtttgttg gtaatgctga ctttcaacgg gcatcatatt taaggtgtgt
     7021 tataaaacct ggttatagtt ttttaattgt attacctttg caaaaaattt cagaaaagca
     7081 ctaattttct taatacctta aataaaatgg caaaatcaaa gaaatttatt acatgcgatg
     7141 ggaactacgc tgcagcccat atagcctaca tgtttagcga ggttgccgcc atttatccca
     7201 ttactccttc atctaccatg gccgagtacg ttgatgaatg ggccgccttc gggaggaaaa
     7261 acatttttgg tgatacggtt agggttgttg agatgcaaag cgaagctgga gctgctggtg
     7321 ctgttcacgg atcgcttcag tccggagcat taacttcgac atttacggca tcccaggggc
     7381 ttcttttgat gatacccaac atgtataaga tttcgggaga gctgcttcca ggggtatttc
     7441 acgtttctgc ccgcagcatt gcagcacacg ccctttcaat tttcggagac catagcgatg
     7501 ttatgagcac ccgtcagtgt gggtttgcca tgttggccac cagcagcgtt caggaggtaa
     7561 tggacctggc cggagtagcc catctctcgg caatcaagtc gagaattcca ttcctgcatt
     7621 tcttcgatgg gtttagaacc tcccatgaaa ttcagaaaat cgaggtgctg gagaacgatg
     7681 acctagctcc attgctcgac caaaaatcac ttcaggagtt tcgcgacagg gcattgaacc
     7741 ccgaacatcc cgtaacccgc ggcacggctc aaaaccccga tatatacttt caggccaggg
     7801 aggcttccaa caaattttac atggatgttc ccgatatcgt tgagggctac atgcaggaga
     7861 ttgccaagct taccgggcgg gagtaccacc cattttcatt ctatggcgat cctaatgccg
     7921 aaaatatcat tattgccatg ggttcggtta ccgacaccat tcgtgaaact attgactacc
     7981 tcatggctaa aggtgaaaag gttgggatga taagcgttca tttgtacaga cctttctctg
     8041 cgaaatactt ctttaaggtg ctgccttcag ctgtgaaacg catagcagtg cttgatagaa
     8101 ccaaggaacc cggggccaac ggcgatccgt tatacctcga tgtaaaagag ctcttctatg
     8161 ggagggagaa agctccgata gttgtgggtg gccgttacgg gctcagctca aaggatacta
     8221 caccagccca aatcattgca gtttttgata acctcaagct tagcgagcct aaaaatcaat
     8281 ttaccattgg catcattgat gatgttacct ttaagtcgtt accaaccggt aaagaggtga
     8341 accttgcacc taaaggaacc tttcaggcca aatttttcgg acttgggtcg gatggaaccg
     8401 ttggggctaa caagaactcc attaaaataa ttggtgataa taccccaaag tactgtcagg
     8461 catactttgc ctacgattca aagaaatctg gaggcatcac cgtttcccac ctgcgttttg
     8521 gcgatcagcc aatccgttcg ccttacctgg taattactcc cgattttgtt gcctgccacg
     8581 ttcaatcata ccttggtaag tatgatgtgc ttaagggttt gcagcccggt ggaacattcc
     8641 tgctcaacac tatctggagt aaggatgagc tgaacgaaaa gctaccaaac tctattaagc
     8701 gttacatggc tgaaaatagt attaagttct acataattaa tgctactcag attgccgagg
     8761 agattggttt gggtaaccgc accaacacca taatgcagag cgcatttttt aaaatttcgg
     8821 gtgtaatccc ctacgatgtg gctgttaagg agatgaaaaa ggccattgag aaatcatacg
     8881 gacgtaaggg agaagaggtt ttgaggatga attttgccgc cgttgatcgt gggggagatg
     8941 ctattcaggt tgtagtgcct atggaatggg caaaacttca gcctgagaat aaacttgaaa
     9001 ttgagggtgc ccctgagttt atcaataagg tggtgctccc aataaacctt caaatgggtg
     9061 atgatttgcc cgtgagcgca ttcgttggtc gtgaggatgg aactttccct gccggaacaa
     9121 cccgttacga aaagagggga attgcagtta atgttcctga gtggatttcg gaaaactgca
     9181 cccagtgcaa ccagtgcgct tatgtttgcc cccatgcagc cattcgtccc ttccttttaa
     9241 ccgaggaaga gttgaaaaac gcacctgcag gaaccaaaac tgttagggga aatggcggaa
     9301 ttaaggattt caacttcagg gtacaggtga gcgttctcga ctgcaccggt tgcggcaact
     9361 gtgttgaggt ttgcccctcc aaggaaaagt cgctggttat gcgaccaatt gaatcacaaa
     9421 tggccgaggc agccaggttc gattacatgc acgaaaaggt gggctacaag gatattcttg
     9481 gtaaagaaaa atcggtaaag aacagccaat ttgcccagcc attattcgag tttagcggag
     9541 cttgcgctgg ttgtggcgaa actccttaca taaaggctat tacccagctc tatggcgacc
     9601 gcatgattgt ggcaaatgct acaggttgtt cctccattta cggtggctca gctccatcta
     9661 ccccatactc tgccgggcct gatggcagag gtgtggcttg gtcaaactca ttgtttgagg
     9721 acaatgccga gtatggtttt ggaattgcaa ccggagtggc caagctccgt caacgcatag
     9781 ctcttaaact tcaggaagca ctagaaggta attttaccag cactacgaag gaggctatga
     9841 aggaatggat tgccggtaag gataatgctt tcctttcaaa agccgcttca gccaagctga
     9901 ttgccgaact ttccaaggaa agcaacccgt tggcaaaaga gctgctcagc ttaaaacaat
     9961 acttcgagaa aaaatcaatc tggattttcg gtggtgacgg atgggcttac gatataggtt
    10021 acggggggct ggaccatgtg ctctctacgg gtgaagatgt caatgtgctg gttctcgata
    10081 ctgaggttta ctccaacaca ggagggcagg cttccaaatc aactccagct gctgctgttg
    10141 ccaagtttgc cgcttcgggt aaacgtgtac gtaaaaaaga tctcggcatg atggccatgt
    10201 cctatggata tgtttacgtt gcgcaggtgg ctatgggcgc cgatcacaac cagtacctta
    10261 aggctattaa ggaagccgag gcatatcccg gaccatcgct tataattgcc tattcgccgt
    10321 gtataaacca cggtattcgc aagggaatgg gttcaactca aagtgaggcg aaacaggctg
    10381 ttaagtcggg ttactggcac ctgtaccgct acaaccccct acttgaggct cagggacaaa
    10441 acccattcca gctcgattca aaagagcccg attggggtat gttccaggat ttcctgaagg
    10501 gcgaagtgag gtacacctcc cttcaacttt ccttccccga tcaagccaac gagctgttta
    10561 aagctgccga ggagaatgct aagtggaggt acaactccta taaaaaactt gctggcgtgg
    10621 ttgagaagcc ttaatttgat tgtgtagaag gtattttagc tgatcacatt ttcttataga
    10681 ccatttgtaa aaggctttcc tgattgcagg gaagcctttt tacgtttaaa ataacttgca
    10741 aaagtgatta tgaaatttgc catatttaga tgggcaaatt ttttttcatg caatgttttt
    10801 ttacttttat aagccgatag gaattttttg cgattcgcaa aaagatatca accggggatg
    10861 attataaagt tactcaataa aaatcctgtg gcatgttgtc taagataaaa aattatttag
    10921 caaagttgca tattccctac tttccttttg cccgctatct taaagcagga ggaagtgcca
    10981 tagggtttat gttgataggc ataagcggtg ggcatagcca aatcgtgtcc cagcctgtaa
    11041 cacgtcatat tacagttgat gaaggccttg ccgcaagcga agtttaccat gtttttcagg
    11101 attcccgtgg atatatctgg tttggaacta acaacggcgt tagccgtttt aacggctatg
    11161 agttcgagaa cttcgacttg gtatcggggt tgcctgataa tacggttttt gaaatctacg
    11221 aggattataa gcacagaata tggtttatac cgttctcagg ttccctctca tactatgaaa
    11281 acggagaaat aaagagctat aggtacaact caaaaattgt tgagcatctt cccaacagca
    11341 ggggcccttc taagctgtca ttttttgtgg acagcttgga caatgtttct tttggtctaa
    11401 aggacttcgg aatcatttca atagaccagg aggggattta caagcatatt gacgatagca
    11461 acaccgatgc ccaagttgtt gtcagaagcg tagggtcggg aaaattttta gtttccgatt
    11521 cacatgaacc tcggaagtat ggcctattct actttgatgg aaggcagtca tacaggttta
    11581 ctttaagcga gctgggcatt aagccgttta agccgattta cttctatgca cagcaagcaa
    11641 aggatggctc ggcttttatc accatcgacg gtaatatttt taggataaag gatggcaggg
    11701 ttgtaagcca taatcattcc attacgcaat cggtaatatg gattagcatt gacgctgata
    11761 acaacctgtg ggtatctgcc tttgaaggag gggtttacat gtttcccaat tgcgatttgg
    11821 aatcgccgga acggctatgc ttccttaaag actatcaggt tacctcagtg ctcagggatg
    11881 atgaggggtc ctactggttc tccaccctta acgatggtgt tttttatgtt cccgaccacc
    11941 agattgcggt tttctcgaag gaaaatggct tatcggacaa ccgggccaat gctgtttatg
    12001 caaaggacgg taaaatctac gttgggtttg agatgggatt tgttgatgtg attgagaacg
    12061 gacaaatcac ccactacaag cttagcagca aagataacat agggataacc tatgtcagag
    12121 atatctatgg tgatacacta accggcaggg tgctggtagc tgcatttggg cacgtagcct
    12181 actttgaaaa caataagctt aaaaaactta ttccggatag ttttaacctg aatacccgtc
    12241 ggataattgg cagtagggga ggtggctact ggctggctac tgccaagggg ttttataaaa
    12301 ttgagaacaa tagaattgcc tacgattcaa ggagcgatgg ctttaacgga atggtgttca
    12361 gcatggccga ggactacaag ggtgatctgt ggttcaacac agtaaatggt ctttggaggt
    12421 attccgacag caacttgctc tttatggggt cagaaaatca gctgttggca aaaattggcc
    12481 acagcatgtt cttctcacac cgcgactcat cgctatggat tggaactaac ggtgctggta
    12541 tagtcatata tggaaaagat ggcaaggttt cccaaatatc tgctgctgat ggtctggttt
    12601 ccaactccat acactacctt tttcccgaag ggaataggat ttgggctgca acccgccagg
    12661 gattgtccat tcttacactt gttaacgata ccacctataa tatccagaac cttaccgtgc
    12721 tggatggtct tcccagcaac gaggtaacca cagtatttgt aatggacgga atagcttaca
    12781 tcgggaccaa caaagggctg gctattctta actatagtaa cttgggattc aatcacaccc
    12841 caccaaaaat cgaagtatta ggcgtttttg ttgatggacg tcctgtagca cctgtaaatg
    12901 gagcagttca gataaactac tcaagtaatg ttgttaactt ctcttatatc gggctggcct
    12961 ataggaatat gggtaatata ttataccggt accgcttaat ggggctagac tctacatggg
    13021 tatttacccg tagcaccagc tgcatattca gcaacttgaa acccggaagc tacacctttc
    13081 aggttcaggc tcagaattct aacggtatat ggagttcaag aagtgcatcc attgacctga
    13141 ttataaggcc gcctttctgg gagaggtttt ggttcataat tcttgccgcc acgatccttg
    13201 ccggaacggt ttatgctgtt tactcgctga gaatcagcga aattaagcga cgtaacgagc
    13261 ttatcaacag catcaacctc tacaagcagc aatcgctccg acagcaaatg aacccccatt
    13321 tcattttcaa cacccttaac tctatacagt acttcatact cgagagggat acagttagct
    13381 cgcacaagta ccttacaaaa tttgcccgtc tcatgcgctt aacactcgac aactctcttt
    13441 cgccaactgt ttcgctaaga gatgagctag aagcccttag gctttacctc gatttggaag
    13501 ccctacggct cgaaggtaag tttacctact ccattgacta caacgacgac gagtcgatac
    13561 ttaacaataa aattcccacc ctgatgattc agccttttgt ggaaaacgca atttggcatg
    13621 gtataatgct taaaaaggaa aagagcggac atgtgtgggt gaaaatagct gatgagggca
    13681 gcaggattgt ttgcaccatt cgggatgatg gtgttggccg gaccaaagcc cagcgaattc
    13741 aagagcaaac tggtaaggaa tactcctcaa gaggatttca aataaccaag cagcgaatcg
    13801 aattgcttaa taccctttac ggcgaaaagt ttagcataaa gtttatcgac ctcttcgacg
    13861 acatgggcaa tgccagtggg acattggtaa gtatttccat tcctaaaaac ttggatgtag
    13921 tttagcgacc catcagtagc tgctgtcttt ttttgaagag cagcattgtt gcaaacggca
    13981 tgttataata agtgagctgc atgttagcca cttccatagc tgtgggtaaa ccagattctc
    14041 gttttctctt ccatgcgttt gttttgctta gtggttgccc aatttttttt aactttgcaa
    14101 aaagcaagcc ctcatgctta aagccctaat tgttgatgac gaaattgcat cgtttcgcac
    14161 ccttgaagtg cttgtggggc agtttatcag caatgttcag attgttgata ttgcgcgttc
    14221 tgctgctgag gctcttgaca aggtggagag gataagcccc gacctggttt ttctggacat
    14281 cgaaatgccg ggtggcgatg ggtttgattt tcttgagaga tgcgcagcga gaaaatttga
    14341 ggtaattttt attacagcct acaaccgcta tgccattagg gcatttcggt acagcgctat
    14401 agattacctc cttaaaccga ttgatgttga tgagctaaga gctgccgttg agcgtgcaat
    14461 taaacgtcgc tatgcaaact tcgacgggca caacaagtac tacgccctgt ttgaaaattt
    14521 gcgctcggta atcccatcaa agctggttgt tacagccaac caggtatgcg agtatatcaa
    14581 cctaaacgac gtgtgcttcc tggaagacga cgataaaatg acccgaataa cctttaccgg
    14641 tggtaacacc atgagggttg aaaaccagct aagctacttt gaagaaatac taggcgaccg
    14701 tcatttttat cgaattaatt caaagcagct ggttaacgtg gaccacatca gacgaattgc
    14761 caatagggca agcgataacc tggagctgga caatggaaca ctgttagctg tagatgtcgg
    14821 gttaaaagca ggcctggaga ggtgcctgga aaaatatatt aaattgggga aagagtagtg
    14881 ggacgaattt tagcactcga tattgggcaa aaacgaacgg gaattgcggt aacagatcct
    14941 ttgaagataa ttgccaacgg gttgactacc ataccctccc atacggtact cggtttttta
    15001 gccgattact ttagtaagga aagtgttgaa cttctggtag ttggtagccc taaacagatg
    15061 gataactctt catctgaggc tatgaagttt atcgatccaa ttgtgcgcgg gattagaaag
    15121 acttttcctg gtatgcctat tcagtttatt gatgagcgct ttacctctaa aatggcaaag
    15181 caggccatga ttgatggggg catgaaaaaa atggatcgcc gcaacaaagc tatggttgac
    15241 accattagcg caacaattat tcttcagtcg tatctcgaaa gcctttcgtt taatcgttag
    15301 gagggcttag gttaaacttt tttgaaatag gcttgttaaa aaatgatgta cagctttatt
    15361 tatttgctat gattcttcct atttacgttt acggctcgtc aattcttaga aaagtggctc
    15421 aggacataaa ccctgactac cctaacctga aaaggttgat tgacgatatg tttgaaacca
    15481 tgagccagtc cgacggaatt ggcctggcag cacctcaagt tggcctatct ataaggttga
    15541 tagtaattga tggtactccg ctagctaaag atgacccctc cctaaaggat tttagaaagg
    15601 tgattatcaa cccatacatt gaggaacgtt ttggagaaaa ggtgatttac agcgagggat
    15661 gcctcagcat tccgaatatc catgaagagg tagagcgtga ggagaaagtt cggattagct
    15721 atctggacga aaattttacc gctcacaacg aagtttttga aggctttgct gctcgcattg
    15781 tccagcacga gtacgaccac ctggagggaa tcctctttac cgatcgcctt tcgcctatcc
    15841 gcaagcagct gctgaggtca aagctaagcg caatttccaa gggaaagttc accgccgatt
    15901 ataagtgccg tctggttaag tagcgttgcc tgcaatctat cgattactgg caactttaat
    15961 ttgtaaataa tggcaatggt gattgcccaa atattctata tttttttcgg tttatttcga
    16021 cggtaatcta catccactat tgtaggataa tatcctttac atgctcacgc aatcaattgc
    16081 agaacccgag ctgttaatgc attcctattt gattaacaaa ttttgtattc aggtagtatt
    16141 gtttatttaa caagttgatt gtaaatttat ttggttattt caaactaaaa ccaaacaact
    16201 atggacaaga aacttgtatt caaggatgtg ctatccaacg gtgttagcac ggggctaaag
    16261 aactttcttt cgctaatcgg ctgcgtggtg ctctggttgc ttaccgtttg gattccctac
    16321 ataaacgttg ggacaaccat tgccattatc acccttcccg cagcactgag caagggtgca
    16381 gtcatttcac ccctcgaaat atttaataaa aggtatttta ggtacatggg tgaattcttc
    16441 ctggtggagg ggcttgcagg cattataatt ttttcggcaa tgcttttcat gtttttccct
    16501 gccattgtga tgtcaattgc ttacagcttt ggcgctcttc tggtagttga caagggaaag
    16561 ggcgcttcgg aggccttacg gcttagcaac aaggctacct acgggaacaa gtggacaatt
    16621 ttctgggttc agcttgtact catcctcccc atgttcatct ttgcctggat ttggagaccg
    16681 ttagccatta tctatgccat actttatgtg cccttcaccc ttggcgcaac tgcttttatt
    16741 tataaggagc ttactggtga tattagtgag gagtaggata gctgtaaaag attcttgcag
    16801 ctaaaaagaa aaaccccgtc gtgatgagaa ggggtttttc ttagcaacaa cttaaaggta
    16861 tgaacagaac ctactcaata accagaacct tgtttttcgg atactttacg gaatacttca
    16921 ggtcgagttt cttttctgct cccggttcca gcgtcaactc ccaggtaacc tctccatttt
    16981 ctgtatttac cttgcctccc gaggtttcca gaatatttac ttcaatctcc tcagaggttg
    17041 ataccggaac ctggtccatc acagttatgc ttattttctg aagcttatta tttttaacgg
    17101 tagtgcgcca tgttcgtgtt tcctcctttt tggtgcctac cagctggcgg gtggtgtagt
    17161 ctttaatttt ttcgcggtta atgctaatgc ccttatccct accaagtgat acctgaaggg
    17221 tgtctgttgc cctggagata tcgagaacag tagcgccaac aaaagtattc tcaaaaaata
    17281 tgttggcctc accatcgagc aggctatact tttcccagtc ggttattctg gccaaaagga
    17341 atgcactcct gtcaattttg ggaacgctgt agtactcgta catggcgggc aggctatagg
    17401 taaccatttc aaccgtatgg tttttgttgt ccgagtctac cgtgtagggt tcacgaattt
    17461 caaaatcaac ggcagtaggt ttttgagcaa ttccctgttg gccaaccact ccgccagctt
    17521 catccatata ggttgcttca tcggctgatt ttgagctggg agcagcaagc tttcgctttt
    17581 cggcggttat tccgtaagcg gttactacca cctcctcatc aagcatcata ttattctcgc
    17641 tcaacaccac attcatgata gggttaaccg gaagtgtttg cgattggtag ccaacgaagg
    17701 aaaaggtcag gtagcttgcg cttgccggca gggtaatgta gtagcttccg tcagctgcag
    17761 ttacggtgct aattgttgtt cctgttacgg ttacacttac tcccggcagg gggtttcgtt
    17821 cagaatccat tacccttccc gtaacccttc caagttgctg gctataggtt ggcggtaggg
    17881 tgttgtagtt taagaagtaa gtctttagcg tagggggtac tcctgaaaca tttggctcgg
    17941 aagttgatag cttcaacttc acatttttcc aatcggtttt tgaatcttgc tttatgtttg
    18001 ccttataaac caatacaaca ggttggtcta tgcttaaagc cctgatatcg taagagggaa
    18061 accaacctgc atttccaacc atgaaggtta cctcaaaagt agccgttaca tcatttgacg
    18121 cttcaacgtt aaccatcact tcaccactag gatactgttt ctggctggtg agggtgttca
    18181 gctggttttg aagttcattt tgctttttat tcagctcttc tatcttcttg ttaatttcga
    18241 gttccttcaa cttcagagct gaaattttag tgccgtagaa tgaggatgtt tctctaaagt
    18301 tagctacgct ggtttcctga tttttgccgc caattatcct gttatcctgc atgaacttca
    18361 gctcttcgga gttaatttcc aggttgatgt tttcaagttt caatccctct gctactgtcc
    18421 gaagggtagc ttccagatcg gttagctcct ttggcttctg cagcctatct acaaaatttt
    18481 gttggtggtt aaccgccagc acgcttacac tattgctagc ctttacccga atgctttttg
    18541 catcaatata gggtgaaaga ttcgtaaatt tcagcaagct tttacccttt gaaagctcgg
    18601 tagtggcctt tccggttagc tgggcgccat ctatgaatac cgtaacctcc tcaacctgta
    18661 tctttaggtt tttctccggg agttcctggg tgtaggcatt tcccactatc aaggagacta
    18721 aaaaaaatag ggcaagtagt tttttcattg cttcaagtta ttattagtgt taaataaaat
    18781 tgatttagct gaacttcgtt agttgagaat ttttacttca tcgcttatat ggattgatgt
    18841 aaaacacgta gccgtttcgt aaagaatttc aagatggtaa aggggaaaat tttaggtgaa
    18901 gccatattat atgatttata cggagctaaa ttggtgcaac agctggcttt aaaggtaggc
    18961 aaaaaagttg aaaccagcag tatcaacctg tgggtgacca aaacctggag ggaatggcat
    19021 gtagtgataa ctttttaatt ttttcaacta ttggccgggg aatttgccaa cacggggatg
    19081 tgcacttgtg caagctaatc aaacctttgc tgtatagaat ggatgttacc ctgaagcagc
    19141 tgtcgttgaa tttatgtagt tggaatctac ccaaatgaaa aatattgtag gtttaggagt
    19201 tggatttaaa gcaggaaaaa cttgaaaagg attgatttta cagttgagtg gcataacagc
    19261 gtaacctctt ccaatagtat ttgtgctcaa cgttgcgagg agggtgctgc tgaagggctg
    19321 gttattgccg ccatgtttca ggaaatgggc cgagggcaac gcggcaactc gtgggagagc
    19381 gaagctggca gaaacctgac atttagcata ctgattaggc cagggcatct cagggttgat
    19441 gagcaattct acctgtcaaa agttgctgct gcttccgtgt gcgactgggt gagtgcttac
    19501 gtttcgggcg atgacgttac cattaagtgg cctaacgaca tctacattgg cgattcaaag
    19561 gtagccggca ttctgatcga gaacagcttt agctcggcaa tgctagacac atcggtggtt
    19621 ggcattggaa taaacctgaa ccagcaaacc ttcccggacg
//
LOCUS       DAOPHX010000035        19610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_3010_length_19610_cov_5.901867, whole genome shotgun sequence.
ACCESSION   DAOPHX010000035 DAOPHX010000000
VERSION     DAOPHX010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 19610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19610
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3010_length_19610_cov_5.901867"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="PK502_04140"
     CDS             complement(<1..915)
                     /locus_tag="PK502_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HPH00040.1"
                     /translation="MRNDKAGKISKLSFVGLIITMGIVYGDIGTSPLYVMRAIASSVT
                     TIDNDFILGALSCIIWTLTLQTTVKYVLITLRADNKGEGGIYSLFALVRRKMPKAYII
                     AIIGGSTLLADGIITPAITVTSAIEGLQIVQKQLPTIPIVLIIITGLFVIQQFGTKKI
                     GSSFGPIMTLWFLTLAILGVNQIVQLPVVFKAFNPYYALQLIVEHPSAMLVLGAVFLC
                     ITGVEALYSDLGHCGLKNIQISWIFVKSALILNYLGQGAYLLKNGFNGNNPFFSIMPQ
                     WFVLPGIILATAAAIIASQALISGSFTLI"
     gene            1724..3175
                     /gene="sufB"
                     /locus_tag="PK502_04145"
     CDS             1724..3175
                     /gene="sufB"
                     /locus_tag="PK502_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HPH00041.1"
                     /translation="MNGEQDKILHEVTGSEYKYGFVTQIDTETIPRGLSEEVVRMISS
                     KKGEPDWMLEFRLKAFRHWQTMEMPTWAHLTIPPIDYQDIIFYAAPRKRNASTGGGGE
                     IDPELKATFDKLGIPLEEQQMLSGVAVDAVMDSTSVKTTFRDTLAEHGIIFCSMSEAI
                     REHPDLVRKYLASVVPSTDNFFASLNSAVFSDGSFCYIPKGVRCPMELSTYFRINAAN
                     TGQFERTLIVADDDSYVSYLEGCTAPMRDENQLHAAVVEIVALENAEVKYSTVQNWYP
                     GDKNGKGGIFNFVTKRGICKGKNSKISWTQVETGSAITWKYPSCILMGDNSYGEFYSV
                     AVTNHHQQADTGTKMIHIGKNTRSRIVSKGISSGEGSNSYRGLVKVLKGADNARNFSQ
                     CDSLLIGDRCGAHTFPYIEVDNPTAIAEHEATTSKIGEDQLFYCNQRGISTEDAVGLI
                     INGYAKEVLNKLPMEFAVEAQKLLQVSLEGSVG"
     gene            3361..4116
                     /gene="sufC"
                     /locus_tag="PK502_04150"
     CDS             3361..4116
                     /gene="sufC"
                     /locus_tag="PK502_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPH00042.1"
                     /translation="MLIVNDLHARINDKEILKGINLEVKAGEVHAIMGPNGSGKSTLA
                     SVLTGREMVEVTHGQVTFNGKNLLEMPPEVRSREGIFLSFQYPVEIPGVSMVNFMKAA
                     VNEQRKYRGLEPLSATDFLKLMRERKELVEIDSALTNRSVNEGFSGGEKKKNEIFQMA
                     MLEPKLAILDETDSGLDIDALRIVANGVNKLKSPDNAVIVITHYQRLLDYIVPDFVHI
                     LYNGQIVKSAGKELALELEEKGYDWIKNGNLKG"
     gene            4133..5497
                     /gene="sufD"
                     /locus_tag="PK502_04155"
     CDS             4133..5497
                     /gene="sufD"
                     /locus_tag="PK502_04155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HPH00043.1"
                     /translation="MSKTDTAGLLDDLVSLYLDNHDLITGGSSDTLNKHREEAIQNFR
                     IIGLPSAKNEDYKYSRVEPLFQHEYEKYFTPKKISFRVDDIFRCDIPELDTHLAMVLN
                     GFYLPSSGQLQKLDNGIIVGSLAQAAKDFPEIVAKHYNVYAQCNADGLVALNTAFAQD
                     GIFIYVPKGAVEEKPIQIINLLMSEENLMVNYRNLIILEESSHANILVCDHTLSSKRY
                     LSNVVTEIFTGANSTLEYVRMQNEHNNSSQLSHTFINQEEGSSVKTNTLTLHGGFIRN
                     NLHIALKGEGASAQAFGLYLTDRTQHTDNFTFIDHAVPNCSSFEHYKGILDEQATGAF
                     NGKILVRPNAQKTQAYQSNNNLLLTADARINSKPQLEIYADDVKCSHGATVGQIDVDA
                     LFYMRSRGIGERDAKLMLMFGFAHEIVGQISIPTLRDRLDELVDKRLHGELSRCHTCP
                     MHCC"
     gene            5644..6858
                     /locus_tag="PK502_04160"
     CDS             5644..6858
                     /locus_tag="PK502_04160"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HPH00044.1"
                     /translation="MNVEKIRQDFPILSREVYGRPLVYLDNAATTQKPKAVLDRVAYF
                     YNQINGNIHRGVHKLSEESTQEYENAREVVRNFINARKTSEVVFTSGTTASINLAAYS
                     FGEVFVHSGDEVVVSEMEHHSNIVPWQLLCERKGASLKILPFRDDGQLCIDQLEGLLT
                     PRTKIIAVTQVSNTLGTVNPVKEIVRIAHNHGIPVLVDGAQGVKHGNIDVQDIDADFY
                     AFSGHKIYGPTGIGVLYGKENWLEKMVPWQGGGDMINRVTFAKTTFNELPFKFEAGTA
                     NYIGASGLAAALDYYTKIGVSAATAYESDLIQYAMRKLSEIGGIRIFGTAPDKAAIIS
                     FLVDNVHPYDTGMILDKLGVAVRTGNHCTQPVIDHYGIDGTVRASLAFYNTYDEVDRL
                     VEAVQRVKEIFG"
     gene            6943..7263
                     /locus_tag="PK502_04165"
     CDS             6943..7263
                     /locus_tag="PK502_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HPH00045.1"
                     /translation="MESRNELAIQSDIVKVLKGVFDPEIPVNIYDLGLIYEVDVDSNS
                     KVTITMTLTAPNCPLADDVVDEVHEKVSAIEGVSGVVVNLTFDPPWDKSRMSDEAMLE
                     LGFL"
     gene            complement(7318..7827)
                     /locus_tag="PK502_04170"
     CDS             complement(7318..7827)
                     /locus_tag="PK502_04170"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HPH00046.1"
                     /translation="MKQLAILLVTLVFPLVLEAQGIAEEMFERYAGKPGYTSVAIGSE
                     FLNFVAALDREDNELQDLSNKISSLKILVAKQQVEEFGKTLERGITREGYKEVMQAND
                     EGGLVTFYALPKGDKFSNFILHVTNSDEEVMLAIAGNFTLSDLSKLGGSSEDDDSYIS
                     LLRKLENEK"
     gene            8074..8991
                     /locus_tag="PK502_04175"
     CDS             8074..8991
                     /locus_tag="PK502_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPH00047.1"
                     /translation="MTLKSQNRAYVYASLVVLFWGTSATAFKIGLSYLSTVQLLFWSS
                     LAATTILFLVLCSQHKFKQLSAATPAQLGRSVMLGFLNPFLYYLVLFKAYSLLPAQVA
                     QPLNYIWPIVLVLLAAIFLGQKLHWYDLLALLVSFTGVTLISSQGSINIFSHSNPLGV
                     VLALSSSIVWATFWIVNMQKNAGDEVVTLFLSFASASIFCLIAMVTLGGFAKPLPWQG
                     ILASAYIGAFEMGITFVLWLKALNLATSTAKLGNFSYLVPFVALIFISLILNEKIIWT
                     TVAGLICIIGAILFQQYLGRKLDNEGQKP"
     gene            9085..10344
                     /locus_tag="PK502_04180"
     CDS             9085..10344
                     /locus_tag="PK502_04180"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPH00048.1"
                     /translation="MNWHKILSLAVIAAMVLSSCDNEDEQKVNVNQEVYNLMDDMYYW
                     YDQMPEVDPNAYKDPVELVNALRVYPPDRWSYVTTKAEFDAYYNQGAYVGFGFGSGFS
                     ANNELYITFVFKNSPLAAMGIDRGWQIISIDGQTPTSENFSSLIGPSEVGVSKKFLFK
                     SPEGSTMEQIFTKSQIEMNSVLMDSIYTTGTFRVGYFVLESFIGPTEAELTGLFSKFK
                     EEGINELVVDLRYNGGGEVGVAKYLASLIAGSSAFGKVFGTYYHNDKHQGDNSSITFT
                     YEDNSIPLNRVVFITTSGTASASELVINGLKPHIEVSLVGSKTHGKPVGMYSFEFRDP
                     SIDWVIVPICFSIRNSNNEGDYFDGIPVDVSAADDITHPFGSMDEESLNAAFTHLGVT
                     AKGYTRKAAVGVQPFVGKGLQGEIGAW"
     gene            10453..11022
                     /gene="ruvC"
                     /locus_tag="PK502_04185"
     CDS             10453..11022
                     /gene="ruvC"
                     /locus_tag="PK502_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPH00049.1"
                     /translation="MTNSTPAEKIILGIDPGTNILGYGIVRCSGKNDLKLVVLGVIEL
                     KKYEDHYLKLKAIYDRVQQLADQYLPDEVAIEAPFYGKNVQSMLKLGRAQGVAMAAVL
                     SRSIPIFEYAPRKIKQAITGSGSASKEQVAAMLKFILGEKDFSQEYLDATDGLAAAVC
                     HFFQGATVGASRKASSWSDFVRQNPGRVK"
     gene            11178..12434
                     /locus_tag="PK502_04190"
     CDS             11178..12434
                     /locus_tag="PK502_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPH00050.1"
                     /translation="MRNDKINKNKIVNDPVHGFISINYPLIFDIIEHPYFQRLRGIKQ
                     LGLTYLVYPGANHSRLQHALGAMHLLDTAIDTLRSKGFVISEEEAEAACAAILLHDIG
                     HGPFSHTLENTIVPGVSHESISLAFIRSLNEEFNGRLSLALEIFEDRYHRKFLHQLIS
                     GQLDMDRMDYLKRDSFFSGVVEGSISTERIIKMLNVVDDNLVVEAKGIYSIENFLIAR
                     RLMYWQVYLHKTVVAAEQTLVKLLQRAKQLVRSGFPMNISAPLLYFFAHDVTGDDFFT
                     PQSKALSYFAMLDDSDIMVAAKDWMNCSDPVLAKLSSMIINRRLLSIELATIPSSSSR
                     LAELKGLFSSAFPNFTEYTDYFVFSDSVGISAYQPGEESIQILHSNGQLVDIAEASDI
                     LKSNALSNETKKYFTCFPKELREILR"
     gene            12471..13517
                     /gene="lpxD"
                     /locus_tag="PK502_04195"
     CDS             12471..13517
                     /gene="lpxD"
                     /locus_tag="PK502_04195"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPH00051.1"
                     /translation="MEFTAQVIANFLGGVVEGNPEVKVSTVSKIEEGSPGSLAFLANP
                     KYTPYIYTTNASIVLVNADFVAEQPVKATLVRVKNAYEAFASLLQLYAQQTEPQLSGV
                     ETPSFIHPSAAYGDDFYLGAFAYISGDVRIGSRVKIYPQVFVGKGVIIGDNTTIYPGA
                     VIYHECTIGSNCIIHAGAVIGADGFGFASQEDANYKKIPQIGNVVLEDYVEIGANATV
                     DRATMGSTILHRGVKIDDHVHIAHNVEVGENTVMAAQCGVAGSTKIGKNCMFGGQVGA
                     SPHIKIADGVMAGAQSGIAGDIRKPGTIILGSPAFDVSKQRRSMAVYKNLPDMANKLN
                     EMQRTIEELKKKLL"
     gene            13626..15023
                     /locus_tag="PK502_04200"
     CDS             13626..15023
                     /locus_tag="PK502_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HPH00052.1"
                     /translation="MADKQRTISQPVTLTGKGLHTGVDVEVTICPAEVNTGVNFQRTD
                     LEGQPKIEAVADNVIDTSRGTSIGTKNVKVNTIEHMVAALYGMGIDNALVKVNGPEMP
                     IMDGSSRFFVEAIASVGTVEQGAIRNYFTIKEKMVFSDEKNGIEIVAYPDDGFSIDLM
                     VDYNSRVLGHQYAKMGNIDEFASQIAPCRTFVFFHELEVLLKHNLIKGGDLENAIVII
                     EHEVPQPELDRIADLFNKPHVKVKPEGILNNLDLHFDNEPARHKLLDMVGDLSLVGVR
                     IKGKIIAIRTGHWANTEFAKILRKQYKKELLKPSAPEYNPNVPPLMDINQIKKTLPHR
                     PPFLLVDKIISMNGTSVVGLKNVTMNEAFFTGHFPHEPVMPGVLQIEAMAQVGGILVL
                     NTVPDPENYTTYFLKIDQVRFKHKVVPGDTIIFRLTLNEPIRRGIANMTGQAFVGNQL
                     VMEGVLMAQIIKNPN"
     gene            15023..15826
                     /gene="lpxA"
                     /locus_tag="PK502_04205"
     CDS             15023..15826
                     /gene="lpxA"
                     /locus_tag="PK502_04205"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPH00053.1"
                     /translation="MAQLNAYIHPDAKIGKDVTISPFSYIAGDVIIGEGTWIGPNVTI
                     MDGARIGKNCRIFPGAVISAIPQDLKFKGEVTTAEIGDNTTMRECVTINRGTASKGKT
                     IVGSSCLLMAYSHVAHDCILKNNIILGNGTQLGGEVEIDDFAIVSAHSLVHQFEKIGA
                     HVIIQGGSKVNKDIPPYIIAAREPITFAGINLVGLRRRSFTHQQIADIQEIYRIIYNK
                     GLNVSQALAFIIDNIPQTAERDLIVNFIKNSKRGVLREYTGPDPNEGID"
     gene            15942..17189
                     /locus_tag="PK502_04210"
     CDS             15942..17189
                     /locus_tag="PK502_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPH00054.1"
                     /translation="MVLIYNLGIRIYLLLIWLVSPVNPKAKSWLAGRRGLLDEVELLA
                     QNNDPKIWVHCSSLGEFEQGRPLIERIRESYPTLKVVLTFFSPSGYKVRKNYSGADYI
                     FYLPLDTKRNAKRFVEAVKPKMAIFVKYEFWYHFLSELHAREIPTYLISANFRETQIF
                     FKPYGRWYRKFLSHFTHIFVQNHLSLELLNSVGIKEVTVTGDTRFDRVSRVASESATI
                     PLLNTFCDGEKVIVAGSTWPKDEELLLNYLSANADRVKMIIAPHEISPSRVGSIVSAS
                     KVEAICFTRPEGTSPKQARLLVLDTIGFLSSTYRYGCLAYIGGGFGAGIHNTLEAAVF
                     GIPVVFGPNYLKFQEAMDLIALGAGFSIKSQAELNDLLDRLLNDPTLLSQAGESAGAY
                     VKSGVGSSEKILSIISNRLNKML"
     gene            17237..17761
                     /locus_tag="PK502_04215"
     CDS             17237..17761
                     /locus_tag="PK502_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HPH00055.1"
                     /translation="MDATRLTPDFYIRDVLLVAPELVGKRICRIYEDGTSSSHIVTEV
                     EAYKGMNDLACHASKGRTPRNDAMFQQGGILYIYFVYGMHWMLNVVASVENEPEAVLI
                     RGVAGCSGPGRVSRLLKIDRSFYAENLGSSQRIWIEDSGLFPSILTAPRIGVNYAGSY
                     WSSRPWRFYTNDYR"
     gene            17953..18029
                     /locus_tag="PK502_04220"
     tRNA            17953..18029
                     /locus_tag="PK502_04220"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17987..17989,aa:Asn,seq:gtt)
     gene            18267..19247
                     /locus_tag="PK502_04225"
     CDS             18267..19247
                     /locus_tag="PK502_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HPH00056.1"
                     /translation="MPKVLGLGNALVDIMTQLPNDGLLETLKLPKGSMQLVDEGFMRK
                     AISFTRDMPQSLSAGGGAANTIHGLANMDVATSFIGKIGDDVFGRAFKTSMEEVGINP
                     IMLQGKAESGRTLALVSTDSERTFAVYLGAALELTAVDLKPEMFEGFDYFHVEGYLVQ
                     NHEMLLRALEIASAKGLQISFDLASYNVVEENRDFLNEIVKKYVTILFANEDEAKAFT
                     GKKPREAVEVLAEIVPIAVVKLGPSGSYIKRNSDFYQVGIIDVSSIDTTGAGDLYASG
                     FLYGLMKGLPLDKCGQIGAILSGHVIEVLGPKMDGERWTTIRRMVAEAET"
     gene            complement(19343..>19610)
                     /locus_tag="PK502_04230"
     CDS             complement(19343..>19610)
                     /locus_tag="PK502_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00057.1"
                     /translation="VQRIPILLKKDVKYRFTVCNSKDYEGKVVLQLFDNNRMLATTYV
                     IGTGKDYPSIDWVCTKTGAYHLFFSFRDGKAGLAVGLLSLVEMM"
BASE COUNT         5429 a         4230 c         4570 g         5381 t
ORIGIN      
        1 gatcagggtg aatgaaccgg atattaaggc ctgacttgca ataatggcag ctgcggttgc
       61 aagaataata cctggaagca cgaaccattg gggcataatc gaaaaaaatg gattattgcc
      121 gttaaaacca ttttttaaca ggtaggcacc ttgccccagg tagttaagaa ttaaagccga
      181 ctttacgaat atccagctaa tttgaatgtt tttcaaccca caatgcccca aatcggagta
      241 gagcgcttct acccctgtta tacaaagaaa tactgcaccc aataccagca tggccgatgg
      301 atgctctaca attaattgca aggcgtagta gggattaaac gctttaaaca ccacgggtag
      361 ctgaacaatc tgatttactc ccagtatcgc cagcgttaaa aaccatagtg tcattatggg
      421 gccaaatgag ctaccgattt ttttagtgcc aaactgctgg ataacaaaaa gtcctgtaat
      481 tatgatgaga acaatgggaa tagtaggaag ttgcttttga acgatttgta aaccttcgat
      541 ggccgatgtt acggtaattg caggtgtaat gataccgtca gccaggaggg tgctcccccc
      601 gatgatggca ataatatacg cctttggcat cttcctcctt accagcgcaa acagggagta
      661 gatgcctccc tcacctttgt tgtcggctct aagggtgatc agaacatact taacggtggt
      721 ttgaagggtt agcgtccaga taatgcagga caacgcaccc agtatgaagt cgttgtctat
      781 ggtagttacg ctgctggcga ttgcccgcat cacgtaaagc ggggaggtgc caatatctcc
      841 atacacaata cccatggtta tgattagccc gacaaagctt aatttcgata ttttccctgc
      901 cttgtcattt ctcataagtc aacctttaat ttttatacct aacatttata ctattgctat
      961 cataaaaatg taatccggcg gtcagcttca cgttgaacct tagaatttca tctactcgct
     1021 aaatcttgca acccaacgct ttactagtgc acaaatatca agattattag ggttaatcat
     1081 gattttattt ggagtagcga tgaccttttg ggtatctatc tatactgatg ggtgaattgc
     1141 caggttaaac cagtacggtt gtttaaagat tgcaagactt taactggtaa aacagcctca
     1201 ttaacaacag catgaactta ttttaagcta aaatgggtaa ttttttgaac ccgcaaaagt
     1261 ataaaagaag gccataggat gtcatcaatt tataaacttt ttattagaac aaatttttaa
     1321 aggtataagc ctgctataat gctatttatg agattacgcc cgatgaacta agacggctat
     1381 tattcctgca ctgttccgga agccatgact ttaagttgtt gccatgttag ctcataccat
     1441 tggggcaacc cataacggat gaattctaca tttgccagcc tatctgacct gtattccggc
     1501 gatttacatt caaatttcct tgtaaattag ctttcagcca aatatctttg caggagcaac
     1561 actcttctga ttcagccacc gattggagtt gttttatgct acgattaaac ctatgtttga
     1621 tgggtaatat agaagttgtg gcgtggttgc gtggcattct tgttatttat taaacatatc
     1681 accagtagca ttgtttacat cctgataatg tttgaacaac ggtatgaacg gggaacagga
     1741 taaaattttg catgaggtta caggttcgga atacaagtac ggttttgtaa cccaaataga
     1801 cactgaaact atccctcgag ggctcagcga ggaggtggtg aggatgattt cgtccaaaaa
     1861 aggcgaaccc gactggatgc tggagtttcg cttaaaagca tttcgccact ggcaaaccat
     1921 ggaaatgccc acatgggctc atttaacaat accccccatt gactaccagg atataatttt
     1981 ctatgctgct ccgcgaaaac gaaatgcttc aaccggaggg ggcggggaaa ttgaccctga
     2041 gctaaaggca actttcgaca agctaggcat tccccttgag gagcaacaaa tgctttcagg
     2101 agttgctgtt gatgcagtta tggacagcac ttcggtgaag acgacatttc gggatacttt
     2161 agccgagcat ggcattattt tttgttcaat gagcgaagcc attcgcgagc atcccgactt
     2221 ggtgcgtaaa tacctggcct ctgttgttcc ctccaccgac aacttttttg cctccctcaa
     2281 ctctgccgtc ttcagcgatg gttcgttttg ctatattcct aagggagtgc gttgccccat
     2341 ggagctaagc acctacttta gaataaatgc tgccaatacc ggacagtttg agcgcacctt
     2401 gatagttgcc gacgacgata gctacgtttc ctaccttgag gggtgcactg cccccatgcg
     2461 cgacgaaaac cagcttcatg cagcagtggt agaaattgtt gccctggaaa atgccgaggt
     2521 gaagtattcc actgttcaga actggtatcc aggcgacaaa aacgggaagg gcggtatatt
     2581 caactttgta accaaacgtg gcatttgcaa gggaaaaaac agcaagattt cgtggacaca
     2641 ggttgaaaca gggtcggcca ttacatggaa gtatcctagc tgcatactta tgggcgacaa
     2701 ctcctacggt gaattttact ccgttgctgt aaccaaccac catcagcagg ccgacacggg
     2761 aacaaaaatg atacacatcg gcaagaatac ccgtagccga atcgtttcaa aaggaatttc
     2821 gtcaggtgag ggcagcaata gctaccgtgg gctggttaag gtgcttaaag gggccgataa
     2881 tgcgcgcaac ttctctcagt gcgactcctt gctaattggt gataggtgtg gtgcgcatac
     2941 ctttccctac atcgaagtgg acaacccaac cgccatagcc gagcatgaag cgacaacttc
     3001 aaaaatcggt gaggatcagc ttttttactg caaccagcgt ggaatttcaa ctgaggatgc
     3061 cgttggtcta attattaacg gatatgcaaa ggaagtgctc aacaagctgc ctatggagtt
     3121 tgccgttgag gctcagaagc tccttcaggt gagcctggag ggcagcgtag gttagaaaca
     3181 ttcgaagaaa ggtgaaatgg taaagctact tgaatactac cggcttaatc gcccgtataa
     3241 cctactcgtt tggaattgaa ggcctcagat ataccggttt acatgaattt tgatgtgtaa
     3301 ttttatggtg ggtcaacacg gaaatattaa tttggaaaca tatacattaa tcatcatagt
     3361 atgctaatag tcaacgattt acatgctaga ataaacgata aggaaatatt aaagggtata
     3421 aacctggagg ttaaagccgg agaagttcac gccattatgg gtccgaatgg ctcaggcaaa
     3481 agcactttag cttcggtgct aaccggcagg gagatggttg aggttaccca tgggcaggtt
     3541 acctttaatg gaaaaaacct tttggagatg ccacccgaag tgcgttcgcg cgaggggatt
     3601 tttctcagct ttcagtaccc ggttgaaata cccggcgtta gcatggtaaa ctttatgaag
     3661 gctgcagtta acgagcagcg caagtataga ggtcttgaac ccctttcggc aaccgatttt
     3721 cttaagctga tgcgtgagcg gaaggagctg gttgaaattg actctgccct aaccaaccgt
     3781 tcggtaaacg agggtttttc cggtggagaa aagaaaaaaa acgaaatatt ccaaatggcc
     3841 atgctggaac ccaagctggc tatacttgat gaaaccgact cgggtttgga tatcgacgcc
     3901 ctgcgaatag tagccaatgg ggttaacaag ctcaaaagcc cggataatgc cgtaattgta
     3961 attacccatt accagcgctt gctcgactat attgtacctg attttgtcca catactctac
     4021 aacggccaaa ttgttaaaag tgccggtaag gagctggctc ttgaacttga ggaaaaaggg
     4081 tatgattgga ttaagaatgg caacctgaag ggttaagtaa aaagttgaaa ccatgagtaa
     4141 aaccgataca gctggacttt tggatgacct ggttagcctc tacctcgata atcatgacct
     4201 tattactggc ggtagctcgg ataccttgaa caaacaccgt gaagaggcaa tccagaactt
     4261 caggattatt gggctgccat ctgcaaaaaa tgaagactac aagtactccc gggttgaacc
     4321 cctctttcag catgagtacg agaagtactt tactcctaag aagatttcgt ttagagtgga
     4381 tgatatcttc aggtgcgaca ttcctgagtt ggacacccat ttagccatgg tgctcaacgg
     4441 attttacctg ccaagtagcg gccaacttca aaagttagat aatggtatca ttgtaggaag
     4501 tttagcccag gctgcaaagg attttcctga aattgttgcc aaacactaca acgtttatgc
     4561 acagtgcaac gccgatggcc tggttgctct taacaccgct tttgctcagg atggcatttt
     4621 tatttacgtt ccaaaaggtg ctgtagaaga aaaacccatt cagataatta acctgctgat
     4681 gtcggaggaa aacctcatgg ttaactatcg taacttaatt atacttgaag aatcgagcca
     4741 tgccaacatt ctggtgtgcg accatacgct atcttccaag cgctaccttt ccaacgtggt
     4801 aactgaaatc tttacgggag ccaactccac acttgagtat gtaaggatgc agaatgaaca
     4861 caacaatagc agccagcttt cccacacctt tatcaaccag gaagaaggtt caagtgtaaa
     4921 aaccaatacc ctaaccctac acggtggttt tattcgtaac aaccttcata tagccctcaa
     4981 aggagagggc gcttcggcgc aggctttcgg actttaccta accgaccgca cgcagcatac
     5041 cgataacttt acctttattg accatgcagt gcccaactgt agcagctttg aacattacaa
     5101 gggcattttg gacgaacagg caacgggggc tttcaacggg aaaattctgg ttagacccaa
     5161 tgcccagaaa acacaggcat atcaatcgaa caataactta ctgcttacag ctgatgcacg
     5221 gataaacagc aagccacaac ttgaaatata tgctgatgat gtgaagtgct cacacggcgc
     5281 aacggttggt caaattgatg ttgacgcact attctacatg cgctcaagag gcattggtga
     5341 gcgggatgca aagttgatgc tcatgtttgg atttgcccat gaaattgtag ggcaaatatc
     5401 aatccctact cttagagaca gactcgacga gctggtggat aagcgccttc acggcgagtt
     5461 atcgcgatgc cacacctgtc caatgcactg ctgctagctt gcgtgtgctt tggcgatctt
     5521 atagtacatt catttggtgg tttaacctgg tgcatgcatc aattgggcag gttgaaccag
     5581 atttttaaac tagatagaat ggatacagtt aaacaacaat cagccgatgg ccagactcaa
     5641 tccctgaacg tcgaaaaaat tcgacaggat tttccaatac tttcccgtga ggtttacggc
     5701 aggcccctag tttacctcga caatgcggca accacgcaga aacctaaagc tgttttggat
     5761 agagtcgcat acttctacaa ccaaattaac ggcaacattc accgtggggt gcataagctg
     5821 agcgaggagt caacccagga gtacgaaaat gcccgtgagg ttgtaagaaa cttcattaat
     5881 gcccgtaaaa cgtcagaagt tgtatttacc tcgggaacca ctgcctccat aaacctggca
     5941 gcctattcgt ttggagaggt ttttgtgcat agtggcgatg aggtagtggt aagcgagatg
     6001 gaacatcact ccaacatcgt tccatggcag ctgctctgtg agcgtaaagg ggcttcactt
     6061 aaaattttgc cattccggga cgacgggcag ctctgcatag accagctcga agggttactg
     6121 acacccagaa ccaaaattat tgcagttact caggtctcca acacccttgg aactgttaac
     6181 cctgtaaagg aaatcgtacg aatagcgcac aaccatggta ttcctgtttt ggttgatggt
     6241 gcacaggggg tgaagcatgg aaatattgac gtgcaggaca tagatgccga tttttatgcc
     6301 ttttcggggc ataaaattta tgggccaacg ggtataggtg ttttgtatgg caaggaaaat
     6361 tggctcgaaa agatggtccc ctggcagggt ggcggcgata tgattaaccg cgttacgttt
     6421 gccaaaacca ctttcaacga gttgcccttt aaatttgagg cgggaacagc gaactatatt
     6481 ggagcctcag gccttgccgc agcgctcgac tactacacta aaataggcgt ttcagcggca
     6541 actgcctacg agtcagattt gatacagtat gctatgcgca aactttccga aatagggggg
     6601 attcgtattt tcggaacggc acccgacaaa gcggcaataa tctcgttcct ggttgataat
     6661 gttcaccctt acgacaccgg aatgatactt gacaagttgg gtgttgctgt gagaactggc
     6721 aaccactgca cccagccggt aattgatcac tacggcattg acggtacagt aagggcttca
     6781 cttgcgtttt acaacactta tgatgaggtt gacaggctgg tggaagccgt gcaaagagtt
     6841 aaggagatat ttggctagca ggcggccgag cggtatttat gctacctgaa aagcttaacc
     6901 ttagcctgtt aattttgttt ctaccctata aattgtgaaa ctatggaatc gagaaacgag
     6961 ctggctatac aatccgatat agtaaaagtc cttaaaggtg tttttgaccc cgaaatccct
     7021 gtaaatattt atgatttagg actaatctac gaagttgatg ttgactcaaa ttctaaagta
     7081 accattacca tgacccttac tgcacccaac tgcccgcttg ccgatgatgt ggttgatgag
     7141 gtgcatgaga aagtatctgc tatcgagggg gtttcggggg ttgtggtcaa ccttaccttt
     7201 gatccgccct gggataaaag ccggatgagc gacgaggcca tgttggagct gggatttctg
     7261 taaaattaga agaaatcagc tacacatcaa ccttgtcgaa ggatgttcgt tttgagctta
     7321 cttttcgttc tccagctttc ttaacaggga aatgtagcta tcatcatctt ctgaggagcc
     7381 accaagcttc gacagatccg atagcgtaaa gtttcccgca atggccagca ttacctcctc
     7441 atccgaattg gttacgtgga gtatgaagtt ggaaaacttg tctccttttg ggagtgcgta
     7501 gaatgttacc aacccaccct catcgttggc ttgcataacc tccttatacc cttcgcgggt
     7561 aattcccctt tccagcgttt tgccgaattc ttcaacctgc tgcttggcca ctaaaatttt
     7621 caggctgctg attttattcg ataaatcctg cagctcatta tcctccctgt ctagggctgc
     7681 cacaaagttt aaaaattccg aaccaatggc gacggaggtg tacccgggct ttccggcata
     7741 gcgctcaaac atttcttcgg caatcccctg tgcttccagc actaatggga aaacaagagt
     7801 aaccagtaaa attgctaact gtttcatgtg catattttta gttgttttga taagttacca
     7861 aaccatccgc cactagcaat gtgtatatgc tagctgccat ttttaaggat ggttcatttt
     7921 gcttttaatt tcaactatta aatacgatag taaagccaaa cttgctgcat ggcgaattct
     7981 ttattttttg gttaagaggg gtagtatttc ggcatttcct tctccggcta agcgattatt
     8041 tctaacttgc ccaattattt gaactacctt cggatgacac tgaaaagcca aaatcgcgcc
     8101 tacgtttatg cctcgttggt ggttttgttc tggggaacgt ctgctacagc cttcaaaata
     8161 gggctaagtt atctttccac tgtgcagctt ctattctggt catccttagc ggcaactacc
     8221 attctcttcc tggttctgtg ttcccagcat aagtttaagc agctgtcagc agctacacca
     8281 gctcaacttg gaaggtctgt aatgctggga tttcttaacc cgtttctcta ctaccttgtt
     8341 ctttttaaag cctacagcct tttgccggcc caggtagccc aacccctgaa ctacatttgg
     8401 cccatagtgc tggtgctgct ggctgcaatt tttttgggac aaaagctgca ttggtacgac
     8461 ctgctggccc tgctggtaag ctttaccgga gtcacgctga tttcatcaca gggtagcata
     8521 aatatttttt cacacagcaa ccctcttggt gtggttttgg cgctatccag ctctattgta
     8581 tgggctactt tttggattgt taatatgcaa aaaaacgctg gcgacgaggt tgtaacacta
     8641 tttttaagtt ttgcatctgc ttcaattttc tgccttattg ctatggttac tcttggtggc
     8701 tttgccaaac ctctgccctg gcagggaatt ttagcctctg cctacatagg agcttttgaa
     8761 atgggcataa catttgtgct ttggttaaaa gcgctaaacc ttgctaccag cacggcaaag
     8821 ttgggaaact tttcatatct tgtgcctttt gttgctctga tttttataag cttaatacta
     8881 aacgagaaga taatttggac cactgttgcg ggattaattt gtattattgg agcaattctc
     8941 ttccagcagt atttaggcag aaaactcgat aatgaagggc aaaaacctta agtaatatta
     9001 gcatgaggtt aatcataatt aattcagaaa cgttttatgt gctgtatgat aatgatgtgc
     9061 aatccctaat aaatagaata acccatgaat tggcataaaa ttttatcgct ggcagttata
     9121 gcagccatgg ttttatcatc gtgtgataac gaagatgagc agaaggtgaa cgtcaaccag
     9181 gaggtttata atctgatgga cgacatgtac tattggtacg accagatgcc agaggttgat
     9241 ccaaatgcct acaaagaccc tgtggagctg gtgaatgcct tacgagtata tccgcccgat
     9301 cgctggagct atgttacaac taaagccgag tttgatgcat actacaacca gggtgcgtac
     9361 gtggggtttg gttttggctc ggggtttagt gcgaataacg aactttacat cacctttgtt
     9421 tttaaaaact ccccgcttgc cgctatggga attgacaggg gttggcagat tatttctatt
     9481 gatggacaaa ccccaacttc cgaaaacttt tctagcctga taggcccatc cgaggtaggt
     9541 gtatcgaaga aattcttatt taaatctcct gaaggtagca ccatggagca gatttttacc
     9601 aagagccaga ttgaaatgaa ctccgttttg atggattcga tttataccac cggcaccttc
     9661 agggtaggct attttgtttt agaaagcttt attggcccaa ccgaagcgga gctaaccggt
     9721 cttttctcaa aattcaagga ggagggcatt aacgagctgg tagttgactt gaggtacaac
     9781 ggcggtggag aggtaggtgt tgccaaatac ctggcaagtc ttattgccgg cagcagcgcc
     9841 tttggaaaag ttttcggaac ctactaccat aacgacaagc accaaggcga taactcctcc
     9901 ataactttca cctacgagga caattcgatt ccccttaacc gggttgtttt catcactaca
     9961 agcggaacgg cttcggccag cgagctcgtg attaacgggc ttaagcctca tattgaagta
    10021 tcgctggttg gttcaaaaac gcatggcaaa cctgttggga tgtactcgtt tgagtttaga
    10081 gaccccagca ttgactgggt tattgtgcct atatgctttt ctatccggaa ctcaaataac
    10141 gagggcgatt attttgatgg gattcctgta gatgtttccg ctgccgatga tattacccat
    10201 ccgtttggta gtatggacga agaaagcctg aatgccgctt ttacccacct tggagtaact
    10261 gcaaaaggtt atacacgtaa agccgctgtt ggcgttcaac catttgtagg caaaggacta
    10321 caaggggaaa ttggcgcatg gtagggtgtg ctaaacaaaa ctacaggcta taagccaggc
    10381 tttcgaacca gtttaacagg tttacgaggt ctccattttg gtataaatgt ttgttatgag
    10441 taaagtaaat tcatgaccaa ctccacacca gcagagaaga taattcttgg aatcgatccg
    10501 ggcacaaata ttcttgggta cggaattgtg aggtgtagcg ggaaaaacga cctaaagctg
    10561 gttgttcttg gagttattga gcttaagaag tacgaggatc actacctgaa gttgaaagcc
    10621 atttacgacc gggtacagca gctggccgac cagtacctgc ccgacgaggt ggccattgaa
    10681 gccccttttt acggtaagaa tgttcagagt atgctaaagc ttggccgggc tcagggggtt
    10741 gccatggcag ctgttctttc tcgctcaatt ccaatttttg agtacgcacc gcgaaaaata
    10801 aagcaggcta taaccggctc gggaagcgct tcgaaggagc aggtggcggc catgctgaaa
    10861 ttcattcttg gggaaaagga tttctcgcag gagtacctcg atgctaccga tggtttggcc
    10921 gctgccgtat gccacttctt tcagggtgcg acagtcggtg ctagtcgaaa agcatcaagc
    10981 tggagcgatt ttgtgcgcca aaacccgggt agggtaaaat aaatggaagt tatgtctggc
    11041 tatcaggttg aatgaaaatt catgtagaaa acacggagta tttctacaat cgagccctct
    11101 aattactcgg caaatttgtc ttcacacaat gttactggct ttttttgata acgacaacaa
    11161 gttttggttc accatatatg agaaatgaca aaatcaacaa gaacaaaatt gtaaacgacc
    11221 cggttcatgg ttttatcagc attaactacc cgctgatatt tgatatcatt gagcaccctt
    11281 actttcaaag gcttagagga ataaagcagc ttggcctaac ctacctggtt tatcccgggg
    11341 caaatcatag ccgtttacag catgcccttg gtgcgatgca cctcctcgat acggctatag
    11401 atactcttcg cagcaagggg tttgtgattt ctgaggaaga ggccgaggct gcatgtgctg
    11461 ccattttgct tcacgatatt ggacatggcc cattttcaca caccctggaa aacacaattg
    11521 tgcccggtgt cagccatgaa tctataagtc tagcttttat ccgtagcctt aatgaggagt
    11581 ttaacggacg cctttctcta gccctggaaa tttttgagga tcggtatcac cggaaattcc
    11641 ttcaccagct tatttcggga cagctcgaca tggacaggat ggactacctg aaacgcgaca
    11701 gctttttttc cggggttgtt gagggttcca taagcacgga gcgaattata aaaatgctca
    11761 acgtggtgga cgacaacctg gtagttgaag caaaaggcat ttactccatc gaaaattttc
    11821 taattgcccg taggctgatg tactggcagg tttacctgca taaaacggtt gttgctgccg
    11881 aacaaacact ggttaaactc ctacaaagag ctaaacagct ggttagaagc gggttcccga
    11941 tgaacatatc agcccctctg ctgtacttct ttgctcacga tgttaccggc gatgattttt
    12001 ttacgcccca atcaaaggcg ctttcctact ttgctatgct ggatgattca gatataatgg
    12061 ttgcagcaaa agactggatg aactgctccg acccggtgct ggccaaactt tcctcaatga
    12121 ttatcaaccg aaggttactc agcattgagc tggcaactat tccttcaagt agcagccgtt
    12181 tggctgaatt aaagggtttg ttctcctccg ctttccccaa ctttactgaa tataccgatt
    12241 attttgtttt ttccgactct gtcggtatta gtgcctatca gccaggtgag gaatccattc
    12301 aaatactaca tagcaatggg cagctggttg acatcgctga ggcttctgac atcctgaaat
    12361 ccaacgcttt atcaaacgaa accaaaaaat atttcacctg tttccctaaa gaactcaggg
    12421 aaattttaag ataaatagct agctttgcgt tcaattaata cattcttaac atggaattta
    12481 cagcgcaagt catagccaac ttcctcggag gggtagtgga agggaacccc gaggtaaagg
    12541 ttagcacggt ttcaaaaatt gaggagggat ctcctggttc actagcattt ttggctaatc
    12601 caaaatatac tccatatatt tacactacca atgcttccat tgttctggtt aatgctgact
    12661 ttgtagccga gcaaccggtt aaagccaccc tggtgagggt taagaatgcc tatgaggcat
    12721 ttgcctcctt gcttcaactc tatgctcagc aaacagaacc acagcttagc ggtgttgaaa
    12781 caccttcatt cattcaccct tctgcagcct atggggatga tttttacctg ggtgcgtttg
    12841 cctacatttc tggcgatgtc agaatcggga gcagggttaa aatttaccct caggtatttg
    12901 ttggtaaggg ggttataatt ggtgataata caaccattta ccctggtgcg gtgatttacc
    12961 atgaatgcac aattgggagc aattgtatta tccatgcagg agcggtaatt ggtgccgacg
    13021 gttttggatt tgcctcccag gaggatgcta actacaaaaa aataccgcaa attggcaacg
    13081 tagtccttga ggattatgta gaaattggtg caaatgctac tgttgaccgg gcaacaatgg
    13141 gttctaccat tctccatagg ggcgttaaaa ttgacgacca tgttcatatt gcccacaacg
    13201 tggaggttgg tgaaaatact gttatggcag cccaatgcgg agtggccgga tcgactaaaa
    13261 ttgggaaaaa ctgcatgttt ggtggccagg taggagccag cccccacatc aaaatagccg
    13321 atggtgtgat ggccggcgca cagtccggta ttgccggcga cattcggaaa cccggtacaa
    13381 ttattttggg aagcccggca tttgatgtct ccaaacaacg ccggtctatg gctgtttata
    13441 agaatttgcc tgacatggcc aataagctaa acgaaatgca gcgcaccatt gaggagttga
    13501 agaaaaaatt attataatta ttgctatagc cggtttctct ttatattttt tgacttatta
    13561 aaaagaaacc ttgaatatgt agaacccctg gaaccaggga atatgtttaa tttgaaaacc
    13621 tttttatggc agataaacaa cgcacaataa gccagccagt tacccttacc ggaaaggggt
    13681 tgcataccgg tgttgatgtt gaggttacta tttgtccggc agaggtgaac accggtgtta
    13741 actttcaacg aaccgatttg gaaggacaac ccaagattga agcggttgcc gataacgtta
    13801 ttgacacctc caggggaaca tccataggca ccaaaaatgt aaaagtcaac accatagagc
    13861 acatggttgc cgctctttac ggcatgggaa tagataatgc cctggtaaaa gtgaatggcc
    13921 ccgaaatgcc aattatggat ggtagctcca ggttctttgt tgaggctatt gctagtgttg
    13981 gaacagttga acagggtgcc attcgtaact actttaccat aaaggagaaa atggttttct
    14041 ccgatgaaaa aaatggtatc gaaatagttg cctatcccga cgacggattc agcatcgacc
    14101 tgatggttga ctacaactca agggtgctgg gtcatcagta cgccaaaatg gggaatattg
    14161 acgagtttgc cagccaaata gcaccttgca gaaccttcgt attctttcac gagctcgagg
    14221 tgttgctaaa gcataacctt attaagggtg gtgacctgga aaatgccatt gtcattatag
    14281 agcatgaggt tccgcagccc gagctcgaca gaattgccga cctcttcaac aaaccacacg
    14341 taaaggttaa acccgaggga atactcaaca accttgacct acatttcgac aacgaacctg
    14401 cccgccacaa gcttctcgac atggtgggcg acttgtcgct ggtgggtgtt cgcatcaagg
    14461 gaaaaataat tgccattcgc accggacact gggccaatac cgaatttgcc aaaatactga
    14521 gaaagcagta caaaaaggag ctgctcaagc cctctgcacc cgagtacaac cccaatgttc
    14581 ctccccttat ggatattaat caaattaaaa aaacactgcc acatcgtccg ccattcctgc
    14641 tggttgataa aataatttcg atgaatggaa cctccgttgt gggtttgaag aatgttacca
    14701 tgaacgaggc ttttttcacc ggacactttc cccacgaacc cgttatgccc ggagttcttc
    14761 agattgaagc aatggctcag gtgggtggca ttctggttct taacactgtt cccgaccctg
    14821 aaaactacac cacctacttc ttaaaaattg accaggttcg ttttaaacac aaggttgttc
    14881 ctggtgatac tataattttt cgcttaacct tgaatgaacc catccgtaga gggattgcca
    14941 acatgaccgg tcaggcattc gtcggtaacc agctggttat ggagggtgtg ctaatggctc
    15001 aaattattaa aaatccaaat taatggcaca acttaatgca tacattcatc ccgacgctaa
    15061 aattggtaag gatgttacca ttagcccgtt tagctacata gcgggagatg ttattatagg
    15121 tgagggaacc tggattggtc caaatgttac cataatggat ggcgcccgaa taggcaaaaa
    15181 ctgccgaatt tttcctggag cggtaatttc agccatccct caggatttaa agtttaaagg
    15241 cgaggtaaca actgccgaga ttggcgataa caccaccatg cgcgaatgcg ttaccattaa
    15301 caggggaact gcttcaaagg gaaaaaccat tgttgggagc agctgcctgc ttatggcata
    15361 ttcacatgta gcgcatgact gcatactgaa aaacaacata atcctcggga atggaacaca
    15421 gctgggcgga gaggttgaga ttgacgattt tgccatagtg agcgcccatt cattggttca
    15481 tcagttcgaa aaaattggtg cccatgttat catccagggc ggttcgaagg ttaacaagga
    15541 tatcccccca tacataattg ctgcacggga gcctataacc tttgccggta tcaacctggt
    15601 tgggttgcgt cgcagaagtt ttacccatca gcaaatcgcc gatattcagg agatttaccg
    15661 tataatctac aataaagggt taaatgtttc gcaggcactt gccttcatta ttgacaatat
    15721 cccgcaaacg gccgaaagag atctaatagt taacttcata aagaattcta aacgaggcgt
    15781 tcttagggag tacaccggtc cggaccctaa cgaaggaatt gactaaatga tttttcctct
    15841 actaccgggg tttttatact gttagattga ggataggcta tatttcgaag cgttttatct
    15901 ttacttaccc tttttgcccc tctttaattg tttgctgttc tatggtgctt atttataact
    15961 tgggaatccg tatctacctg ctacttatat ggttggtttc accagtaaac ccaaaggcaa
    16021 aaagttggct ggccggacga agggggcttc tcgatgaagt tgaattgctg gcccaaaata
    16081 atgacccaaa aatttgggtt cactgctcat cgttgggtga gtttgagcaa ggacgcccat
    16141 taatagaacg cataagggag agttatccta cgctaaaagt tgtgcttacc tttttttctc
    16201 catcgggcta taaagtaagg aaaaattata gcggagccga ctacattttt taccttcctc
    16261 tcgatacaaa aaggaacgcc aaaagatttg tcgaggctgt aaaaccaaaa atggccattt
    16321 ttgttaagta tgagttttgg taccactttc tctccgagct tcatgccagg gaaattccaa
    16381 cataccttat ttcggccaac tttagggaaa cccaaatttt tttcaagccc tacgggaggt
    16441 ggtaccgtaa atttttatcg cattttaccc atatttttgt tcaaaaccac ctttcgctag
    16501 agctgctcaa ctccgtgggt attaaggagg taactgttac cggtgatacc cgtttcgaca
    16561 gggtgtcgag agttgcctct gagtcagcta cgattcccct tctcaatacc ttttgcgatg
    16621 gtgaaaaggt aattgtggcc gggagtacct ggcccaagga tgaagagcta ctcttgaact
    16681 atctatccgc aaatgccgat agagttaaga tgatcattgc tccacatgaa attagcccga
    16741 gcagagtggg ttcaatagtg agcgcttcaa aggttgaggc catttgtttt acccggcccg
    16801 aaggaacttc gccaaagcag gcacgactgt tggtactcga tacaataggc ttcctctctt
    16861 caacctaccg gtatggctgc ctggcatata ttggcggtgg ctttggcgct ggtattcaca
    16921 acaccctgga ggcagcagtt tttggcatac cagtagtatt tggccccaat tacctgaaat
    16981 ttcaagaagc aatggattta attgctttag gagcgggttt ttctatcaaa tcgcaggctg
    17041 aactcaacga tttgcttgac aggcttctga atgacccgac attgcttagc caagcaggcg
    17101 aaagtgctgg tgcctatgta aaatcgggtg taggctcatc agaaaagata ctaagtatca
    17161 tcagcaatag attaaacaag atgctgtaga tactctggtt attgccggag ggaaaagtgt
    17221 ttaaagtgat gggtaaatgg acgcgacgag actaacaccc gacttttaca tacgcgatgt
    17281 gctactggtt gcccctgagc tggttggtaa gcgtatctgc cgaatttatg aggatggaac
    17341 gagttcaagt catatagtca cagaagtgga ggcctataag gggatgaatg atttggcctg
    17401 tcatgccagc aaggggcgca ctccccgtaa cgatgccatg ttccaacaag gcgggatact
    17461 ctacatctat tttgtctatg gtatgcactg gatgctcaac gtggtggcat cggttgagaa
    17521 tgaacccgag gcggtattga ttaggggagt agcaggttgt agtggacccg gtcgagttag
    17581 caggttgttg aaaattgaca ggagcttcta tgctgagaac ttaggcagct ctcaaagaat
    17641 ctggattgag gactctggcc ttttcccttc cattttaacc gcaccgcgta ttggggttaa
    17701 ctatgctggt agctactggt ccagccggcc atggaggttc tacaccaacg attataggta
    17761 atccctacgt tctatatcac ccattttacc ggaagctatt tgggaataag tgtccttcaa
    17821 caggccatgg taacccgtgt tactttgcca tgatttcagc aatttcgata tgagtgcagt
    17881 gaaaaaaatt ccatttaatt ttggcacgaa ttaatatttt gtagtacatt tgcaccgctg
    17941 aaattaaagc attcctcatt agctcagttg gttagagcac ctgactgtta atcagggggt
    18001 cctaggttca agtcctagat gaggagccag ccccgatgct tatcggggtt ttttctttta
    18061 aaaccggcaa tatttctaac gcttgcagct gaagcgtgaa caaccgtatt tataaggcaa
    18121 cgagttgcat ttttaagggt aagagatttt gaaaattatt aactattcgt tggtattctt
    18181 gacgaaccaa tagctatctt tggtaggctt tgcaagggta aagcaggtat gctgcttaag
    18241 agacaatacc tctcataacg tataatatgc cgaaagtact tgggttaggg aatgcgctgg
    18301 tggatataat gacccagcta ccaaacgatg gattgctaga gacgttgaag cttccaaaag
    18361 gcagcatgca gctggttgat gagggtttca tgcgaaaggc aattagtttt accagggaca
    18421 tgccccaatc gctctcagcg ggcggcggag cagcaaacac cattcatgga cttgccaaca
    18481 tggatgtggc aacctcgttt attggcaaaa ttggagacga tgtttttggg agagcattta
    18541 aaacaagcat ggaggaggtc gggataaatc ctatcatgct acagggcaaa gccgaatcag
    18601 ggagaacgct ggctttggtc agcactgatt cggagcgtac ttttgcggtg taccttggag
    18661 ctgccctgga gctgaccgca gttgatttaa aacccgaaat gttcgaaggt tttgactact
    18721 ttcatgttga aggctacctg gttcaaaacc acgaaatgct actacgtgct ttggagattg
    18781 cttctgccaa aggtcttcag atttcgttcg atttggccag ctataacgtt gttgaggaga
    18841 accgtgattt tctcaacgag atagtaaaaa agtacgtaac cattcttttt gccaatgagg
    18901 atgaagccaa ggcctttaca ggcaagaagc ccagggaggc agttgaagtc ttggcggaaa
    18961 ttgtgcccat agccgtggtt aagctgggcc cttcagggtc gtacataaag cgaaattccg
    19021 acttctacca agtgggcatt attgatgttt cgagtataga tacaaccggt gcgggcgatt
    19081 tatatgcatc cggttttctt tacgggctga tgaaaggatt accgcttgat aaatgcgggc
    19141 aaattggcgc tattctgtcg gggcatgtca tagaggtgct tggccccaaa atggacgggg
    19201 aacgttggac taccataaga agaatggttg cagaagcgga gacgtaatta gctaaacaaa
    19261 aatggatatg ctcaaacgcg ctaaaaaagg aactttaata aatagttctt ttttagctat
    19321 gggtgtaagg tcttttatag atttacatca tctcgacaag tgagagcagg cctactgcca
    19381 gtccggcctt accgtccctg aagctaaaga aaaggtggta ggctccggtt tttgtgcaca
    19441 cccaatcaat ggatggataa tcctttccgg tgcctattac gtaggtggtt gcaagcatac
    19501 gattgttgtc aaaaagctgt agcacaactt ttccctcata gtcctttgag ttgcaaactg
    19561 tgaaacggta tttcacatct ttctttaaaa gaatgggaat tcgctgaaca
//
LOCUS       DAOPHX010000036        18921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_3186_length_18921_cov_5.856355, whole genome shotgun sequence.
ACCESSION   DAOPHX010000036 DAOPHX010000000
VERSION     DAOPHX010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 18921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18921
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3186_length_18921_cov_5.856355"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PK502_04235"
     CDS             <1..269
                     /locus_tag="PK502_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00058.1"
                     /translation="DAPFSEFGDNFVYNFFNIAPQVFTQSIILIKDLYDPKADNYLND
                     KGNKILEKMKNGEINGTFYVNVVEEKADATNLVTKNKCYYPDKF"
     gene            274..774
                     /locus_tag="PK502_04240"
     CDS             274..774
                     /locus_tag="PK502_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00059.1"
                     /translation="MDSSVLRDKIGQKRPRYKERYRSLIESISVKGDTSGKGDFSAFG
                     PFFQTYMYAFVIGYKLGTPKFIEKGEKTADFASFVDWKPTSVRDFILMLLLNDTEKYS
                     VPYSWLELENANEEMIENFISELFRQMEGYANAGFEYLQNKWDNEKIEFQDPFVFVNF
                     LLGLKD"
     gene            779..2110
                     /locus_tag="PK502_04245"
     CDS             779..2110
                     /locus_tag="PK502_04245"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPH00060.1"
                     /translation="MPESQNIEYKSSWHNDYLKWICGFANAQGGKIYIGMNDAATVIG
                     VENQKTLMEEIPNKIKNNMGITAEVNLLHEGDKDYIEIVVQPYSVPISLRGRYYYRSG
                     SVMQELTGAALNDFLLKRAGYTWDDVIDERATFADIDEPTIKKYLGKAEEVGRLPDID
                     GLSTPELLEKLRLAQDGKLKRAAIVLFGKDPGKFYPNTFVKIGKFEDDDFTIRFQEVE
                     EGNIIQVLDKVLRTLDYKFLIRNISFEGMNRVETLEYPITALREMLLNALIHRNYMGA
                     PTQIRVYDKKLSVWNDGGLPPTITLSQLKENHSSHPRNPILAEACFKGGYIDSWGSGI
                     MKIINSCKIDGLPVPDLKEKEGGFIVTLFKDRFSKEQLEKLGLNDRQMKAVEYVKTKG
                     KITNAVYQEINGIGKSVTIDELRDLVEQQIFVRIGETGRGTYYELANKNDR"
     gene            2792..3589
                     /locus_tag="PK502_04250"
     CDS             2792..3589
                     /locus_tag="PK502_04250"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HPH00061.1"
                     /translation="MMKKIHILVIAALLPSFIQAQSDTVFNRTDEQGLKQGLWRKYYS
                     TGVKAYEGWFKDNKPTGEFKRFHENGSLKVILSYRQDSTAWAVFFSPKGNKIAEGKFV
                     GQQKDSLWRYYNPDGTINYDEYYQNGLKNGRCRQYYPDGKLLESAPFTNGKLHGAVIQ
                     FFPEGLNKSVVNFNNGIQDGDLKVFYPSGRKRLEGKYANGLKEGEWIFYGENGKITQR
                     IRYVKGVPENYNELLERETHLLDSLMQNAGRIKEPDFDEMSYGSNYP"
     gene            3601..5232
                     /locus_tag="PK502_04255"
     CDS             3601..5232
                     /locus_tag="PK502_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPH00062.1"
                     /translation="MKYSFRILLIIQLVGLSSAAKPQSKYFIGFTDKANSPYTISNPE
                     QFLSIRSIERRTRQNLALTESDLPVNPSYLDTLANMGITIHFPLRWMNGVVATISTPD
                     QLTQIQNIPFVSGIKEIYTPTKEHKSSKFTIEEPQSQPFSKPDKDAPNYGISATQVNM
                     LNGHILHGNGFLGEGIEIAVLDAGFYNVDSHPVFDSLWMNKRILGTKDFVNPTSNIYN
                     EHAHGMMVLSIMGGYANGQLVGTAPHARYWLIRTEDASSEQLVEEYNWAAGAEFADSA
                     GADIINTSLGYTTFDVPSQNHTYSNLDGQTTVITRAANMAASKGMLVVVSAGNDGTSP
                     WYYISAPADSPKVLTVGAVDGNRNKAAFSSFGPTADERVKPDLCAMGVGTIIATTDGL
                     VGSGNGTSFSAPLISGMMACLWQAKPNLTVSELMESVISASSSFNSPNNEIGYGIPDF
                     ALAMPVGTYSYKPNIRIIPNPFSSQVQIYIPLEIDEAVSCQLLTPSGLTLYMCRQITE
                     NGVLRINIPESLPKGLYIFHIAAGNQAFNLKAIKD"
     gene            5238..6650
                     /gene="xseA"
                     /locus_tag="PK502_04260"
     CDS             5238..6650
                     /gene="xseA"
                     /locus_tag="PK502_04260"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPH00063.1"
                     /translation="MSNGSTRLTLLELNLLVKETLAKSLAGSYWVVGEISEITEHPSG
                     HCYMELVQKGDDTDTIRAKARANIWGYTYRMLKPYFESTTNRPLSRGMKILVNAQVVF
                     HEYYGYSLTVLDIEPEYTLGDIELKRREVIARLISDGVMNMNKELELDMLPQRVAVIS
                     SANAAGFQDFVKQLTHNPHGYAFTYELFPALMQGDEAELSIINALGKIYDKLACFDLV
                     TIVRGGGAQTDLSCFDSYTLASNIAQFPIPVITGIGHDRDVTVADMVSHTSLKTPTAV
                     AEFLIDKFRDAEAVANNLAGSVSQLAGKLLADMKESLTSNQVELQAILNQKVAQNKLY
                     LQQLTSSLPHMAKLVQVLHADELKSLNIRLSIPISRIKANYVKLNAYAGLLKSMPHKL
                     LQNEKIKIEHHFSTLKNLHPDSILHRGFTITYSNGKIVKNLNNLNVNDVITTQFWNGS
                     IESNITKIKASKKLLANNKK"
     gene            6666..8894
                     /locus_tag="PK502_04265"
     CDS             6666..8894
                     /locus_tag="PK502_04265"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HPH00064.1"
                     /translation="MFKADNHPTKLWHLKKFRHILLLASVFSFHTGNILLAQNSSSVH
                     LLDSLLTEAKQTTGIRKITALDKISRYYWEKNPDSSLYYSNLALVEAQRDKSNEALGD
                     AYNSMGNAYSSKHENETAITYYLKSKDYREKSGDWTKVGHSLSNLGLTLRDLKRYSES
                     ISSYRQAAKYYSDAKNYLFEGISYLDIAQVYRQLNDLNRALENAILAINIFLEHDITQ
                     PLARCYNFIGNLHKDLKNFDLAEDYYLKAYEIWSKANNLSGISSALNNLGIIYDEKGN
                     YEKALENYNRSLDIAIQLNDSIGISTAYNNIGFIYVKLNQLERGINAYLKSMAISRQI
                     GEIESYLNTCNNVASVYLKAGMLDKCEEYLNIVLGNLKSITELAYIQETYQLLSELYA
                     KRGNYKKAFEFKSLQLAYNDSIYNQQQVRNILETQTRFETEAKEREIQLLKKDNEIKQ
                     LEFEKQRNFQNILTFITILLAIAIAVTIYISRIIRKKNLLLAEKNRALEEANKKMAET
                     EVNLRELNATKDKLFSIIAHDLKNPFNALLGFSDILERNYNFLSDEERIEYIGVINDS
                     TQSLYKLLDNLLQWTRTQTGTITYIPENFKLLPLVKQEITNVNLNSEKKKISITVNID
                     ENISVFADKNTTATIIRNLLSNAIKFTDLGGHIEIAARYKDQMVETSVSDSGIGINDN
                     DLDRIFMLDGSFTTKGTSNESGTGLGLLLCKEFVEKNNGKIWVESEKGKGSTFFFTLP
                     AK"
     gene            9000..11885
                     /locus_tag="PK502_04270"
     CDS             9000..11885
                     /locus_tag="PK502_04270"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPH00065.1"
                     /translation="MKLVIQKWGVALLLTAYSLIAAQAGSPSSSKVDSLYQRVLAIAD
                     ENPQEASRLANLCRQESSKSGNSRDKALSLMANAQALIAMSLWNNASEKIDSASLFVQ
                     KLNDLNLEADLVDLRSNLEVKVSGSDAALKMLQKAYSQAIKNKRPDLGVKFLLLQAGL
                     YQQKGNLAEANRMREIALDQSSKAGCKKCQAECQQNIASSYWQQGRFNEALESYFKSL
                     ILFEDINDTIGMLAALKGVGISYRDLGQFEKSLTYLNRALKLSDSSNNYTEEAAILNI
                     IGSLYFKFNQVDDALKQYEQSLQIRKKLGLLRSAISTHSNMARAYLQKSMYKQAIEHL
                     NEALALQEQVVDPLAEASTLTEIGNLNLQQGNIAEALRHYLMALKLRQTYGQDEDIAK
                     SLTSIGLTYRRLGMYKSALKYLEQAREIIQDMKGSMNDAAYILQNLGHIYLDLNSPDK
                     AIATYKEALTLKEKSGDSIGAAKILKNIAQAQLKSSQPDQARTSISQALKLVTRTHST
                     SDIADLYNELGNVERQAKNPGMAIKHYHTAIENYKALNNNEGVALCLRKIGEIQVGQK
                     QLDEARKNINSSIEIGKQSDNKYLLSYGYLAQSELYRAQGLFDKALESYIKHIGIRDS
                     LESVKRNEANLEAQIDLELDQTKTEIKVMEAEVEALRQKTALDKAIIEKQRLFRNSLI
                     AIILLVVTAASIAFYSLIQKRRYAKLLEEKIEEIRLVNERLNESESELRQTIKTKDKL
                     FSIIAHDLRSPFTALVGLSEVMATNLESLSPDEIREFSHHIHRSATEVLNLTENLLSW
                     ARSQTGKIELSPKPINLKDLINQVFSVAAIPAQEKKIKLRESIPANLTVFADFDTLQT
                     VIRNLVSNAIKFTPQGGSIGISAKNSSNSMVEIAITDTGVGIDPENQAKLFRVDGFTT
                     KGTNQENGTGLGLILCKEFVELNHGTIEVSSTPGLGTTFTISLPSNN"
     gene            11890..12096
                     /gene="xseB"
                     /locus_tag="PK502_04275"
     CDS             11890..12096
                     /gene="xseB"
                     /locus_tag="PK502_04275"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPH00066.1"
                     /translation="MTKKPQRYIDAINEIEQIITEIENNELDVDDLTEKIRRASELLK
                     FCKTKLRATEEEIQKIISDFEEDS"
     gene            12305..13912
                     /locus_tag="PK502_04280"
     CDS             12305..13912
                     /locus_tag="PK502_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPH00067.1"
                     /translation="MAYSIVAICGSERLSREIKSIVGIAEFDFDLHFVTDDTNIITNL
                     TQLNPDIVLFDVSYSEYAAPDLLTIIKNDIKTESVPILAIADFLQPERIQALFIAGAD
                     DFISQPLAAAELIQRTMIGIQRGRMLKKLKEQSRQFNSITDAISKAGNSMIVINHLGK
                     ITWVNEGFKRLYECPLDYFVSIFGDNLFDSSTNPVTREALDKCRKNGEYVVYESKWVT
                     PQGKEKFIQTSLTPIFDSLQNLSQIIAIETDISDLKAIEKDLEEKHEHLLTITENLKS
                     VNAVLAEHQERIQKQALQLEEEKQKSENLLKNILPWEVAYALQKKGSYKPKKFKEVSV
                     MFTDFVGFSKISSNYGDIDEFLSVLGGFFEVFDEITSQRFIEKIKTIGDSYMCVGGLP
                     RVNRSHPFDTVLAAIEIQRFIHDKAVQAAMEGKPVWNLRIGIHTGSVIAGVIGKRKFA
                     YDIWGDTVNIASRMETASEPGKVNISQTTYSYIKDYFVCTSRGKVPAKNLGEIEMYFV
                     ERIKPEYSEDELGLIPNSSFRKIVASF"
     gene            complement(13928..14422)
                     /gene="cdd"
                     /locus_tag="PK502_04285"
     CDS             complement(13928..14422)
                     /gene="cdd"
                     /locus_tag="PK502_04285"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPH00068.1"
                     /translation="MSESNSFQFRYNRLRGKEELAPNERELVDRAVAALSSSYSPYSH
                     FRVGAAVLLENGEIITGSNQENGAYPSGLCAERVALFYAGARYPGIPVVSMAVVASSG
                     DGLTENPVTPCGACRQVMSEYRAVSGKPFAILMVGSNEIVRVEDVEQLLPFSFSLSNG
                     MKRK"
     gene            complement(14588..16021)
                     /gene="aspA"
                     /locus_tag="PK502_04290"
     CDS             complement(14588..16021)
                     /gene="aspA"
                     /locus_tag="PK502_04290"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HPH00069.1"
                     /translation="MVDAASQYESGSTRVEHDLLGERDVPAEFYYGVQTLRALENFNI
                     SGVTLNFYPDLIDSLANVKQAAAEANFELGLIDEKVRDAIVAACKEITNGKLHNHFVV
                     DMIQGGAGTSTNMNANEVIANRALELMGYSKGEYQHCHPLNHVNLSQSTNDAYPTAVK
                     LAIVCSNAKLIKVLESLIESFRKKGQEFAHVIKMGRTQLQDAVPMTLGQEFEAYAVTL
                     GEEIERLTQNAKLFLEINMGATAIGTGINSDPEYPQKVLKHLRSITGLELVLAANLVE
                     ATQDTGAFVMYSSAVKRLAVKLSKICNDLRLLSSGPRTGLSEINLPPMQPGSSIMPGK
                     VNPVIPEVVNQIAFRVIGNDLTVTMAAEGGQLELNVFEPVIVHSIFESVEMLKNGMNT
                     LKYRCIDGITANEERCRSMVHNSIGLVTALNPYIGYEASTQLAKEALSSNRSVYQLVL
                     EKGLLTREQLDRILAPENMIKPGKMKL"
     gene            complement(16121..16357)
                     /locus_tag="PK502_04295"
                     /pseudo
     CDS             complement(16121..16357)
                     /locus_tag="PK502_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
     gene            complement(16677..17525)
                     /locus_tag="PK502_04300"
     CDS             complement(16677..17525)
                     /locus_tag="PK502_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HPH00070.1"
                     /translation="MLSQVWEQCHASVCSLHFINERGMVIDSLSGFKVNSSLITSEYA
                     FYINNAQKVEISFVEGDANTPTATMRIGYNEFINDLRIGYTNNQSDYAVFNINFSEFE
                     KIPGLTLCECRNFQIGKQVATLGFNFGENNLSIKSAVISSAFSSKNGIRCLELDGLTC
                     FGNSGSPVIDPDSMQVIAIISRRNTPAFKSYMELNRCIVSNIEELRKLEGKLKIDTID
                     PVQVLIANQNQIRLLASNLYKYTATGSSKAIMLDRILSYFNEQAITEQAQISSLEQKV
                     GYGLID"
     gene            complement(17554..17682)
                     /locus_tag="PK502_04305"
     CDS             complement(17554..17682)
                     /locus_tag="PK502_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00071.1"
                     /translation="MKKENSIREKITLKFKDDAGVFTIFFLVTLLFVYALIKALTL"
     gene            17939..>18921
                     /locus_tag="PK502_04310"
     CDS             17939..>18921
                     /locus_tag="PK502_04310"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HPH00072.1"
                     /translation="MVELLTQEVESRFGRKISCQKDCISLSISIMDTCREYLSPATLR
                     RLFGLLATNSNPSHVTLDILSRYAGYTGWDDFVSHKKSKIPEGIIRSADEDWEQAAEH
                     AKAVSSKTLNHIMHLSGIRFEETVHRRQVEERLDQFLNSNNPATAIIAPGGYGKSILL
                     AHWYQIHRDLKANMNDVFLLAPATMLDHYASSNTFIGHWLAQLLGQKSLESLLVKDLP
                     GRIVFIFDALDEVVSVGAKFNKILEALADLAEAFAQTNRFKLVISTRLSTWTQLKPYI
                     THPLLWYFSDSELLTHEGANIPPLSPDEVQQILDQTINLRFNQRLLIFELHP"
BASE COUNT         5868 a         4337 c         3986 g         4730 t
ORIGIN      
        1 cagacgctcc gttttcggag ttcggcgaca attttgttta taacttcttt aatattgcac
       61 cgcaggtatt tactcaaagt atcatactta ttaaagattt gtacgacccc aaagctgata
      121 attatctgaa cgacaaaggt aataaaattt tggagaagat gaaaaacggc gaaataaatg
      181 gtacttttta tgtaaatgtg gttgaagaaa aagctgatgc aactaattta gttacaaaaa
      241 ataaatgtta ttatccagat aaattttaac acaatggata gttcagtatt aagagataaa
      301 atagggcaaa aaaggcccag atacaaagag cggtatcgtt ctttgattga aagtatttca
      361 gttaaaggcg acacaagtgg aaaaggagat ttttctgcat ttggcccttt tttccaaacc
      421 tatatgtacg catttgttat aggttacaaa ttaggaacac caaaatttat agaaaagggt
      481 gaaaaaacag cagattttgc ctccttcgtc gattggaagc caacttctgt aagggatttt
      541 attttgatgc tattacttaa tgatacagaa aaatacagtg tcccatattc gtggttagaa
      601 ttggagaatg ctaacgaaga aatgattgaa aattttatat ccgaattgtt tcgacaaatg
      661 gaaggttatg caaacgcagg ttttgagtat ctgcaaaata aatgggataa cgaaaaaatt
      721 gagttccaag acccatttgt gtttgttaat tttttattgg gcttgaaaga ttaaatttat
      781 gcccgaatcc caaaacatag aatacaaaag cagttggcac aatgattact tgaaatggat
      841 atgcggcttt gccaatgcgc aaggtggtaa aatatatatt ggcatgaacg atgccgctac
      901 cgttatcggc gtggaaaacc aaaaaacgct gatggaagaa attcccaaca aaatcaaaaa
      961 caatatggga attactgccg aagttaatct tttgcacgag ggtgacaaag attatattga
     1021 aattgtcgtg cagccctatt cagtgcctat ttccctgcga ggaaggtatt attaccgtag
     1081 cggtagcgta atgcaggaac tcactggcgc agccctcaac gattttttgc tcaaacgggc
     1141 aggttatact tgggatgatg ttattgacga gcgggcaact tttgcggata ttgacgagcc
     1201 aacaatcaaa aaatatttgg gaaaagccga agaagtaggt cgtttgcctg atattgacgg
     1261 actttccaca ccggaacttt tggaaaaatt acgattggca caagacggaa aactcaaacg
     1321 cgctgcaatc gtgctttttg gcaaagaccc cggaaagttt tatccgaaca cctttgtaaa
     1381 aatcggcaag tttgaagacg acgattttac aattcgtttt caagaagtag aagaaggtaa
     1441 tattattcaa gtgctagata aagttttacg cacacttgat tataaattcc tgatacgcaa
     1501 catttcgttt gaaggaatga atcgcgtcga aacgctcgaa taccccataa ctgcacttcg
     1561 cgaaatgtta cttaatgcgc tcattcacag aaattatatg ggtgcgccta cgcaaattcg
     1621 tgtgtatgat aaaaaactgt ctgtttggaa cgacggaggt ttaccaccaa caattacgtt
     1681 gtcgcaactc aaggaaaacc actcgtcgca cccacgcaat ccgatacttg cagaagcatg
     1741 tttcaaaggc ggatatattg attcatgggg cagtggcata atgaaaatta taaattcctg
     1801 caaaatcgat ggactgccag tacccgacct gaaagaaaaa gagggcggtt ttatcgtaac
     1861 gcttttcaaa gaccgttttt ctaaagaaca attagaaaaa ctcggattga acgaccgtca
     1921 aatgaaagcc gttgaatatg taaaaacgaa aggaaaaatt accaacgcag tgtatcagga
     1981 aatcaatgga atcggcaaat ctgttacaat agacgaattg cgtgatttag ttgagcaaca
     2041 gatatttgta agaattggtg aaacaggtag aggtacttat tatgagttgg caaataaaaa
     2101 cgaccgctaa acgaccgaaa cggttgacaa acgaccgcta aataaccgaa aagtaccggg
     2161 agttattatg aattacacaa aaaaagaatt ggctcaatta actcataatt ggctcaatcc
     2221 cttaatgctg aaagcccgca ttgccagcaa aaccggtaat attgagtcca ataaatgtcc
     2281 ggcaattgca gataaactac cggtattgca gataaactac cggaacaacc ggatgataaa
     2341 gaaatcgcgt ttataagaat aaagcaaaag gaacaacgat cgggtaatag ttgggcaaat
     2401 tgggcaatta gtagtcattt gttagtgaat atagtcgatt ataatagaaa tacaaaacta
     2461 actcggtagc atcccaaaaa gtgtgtaaag gtatctttct atcaatacat aaggtaatca
     2521 tctcccgttt tttgtttgta aaggaagaaa gcgaagcgga ggcgtagcct gagcttcact
     2581 tcctttactc tggccatccc tgttcacatc aactacaatc agggggcaaa caattgaaca
     2641 gctggttttt atccatcagc ctggcataac taaagccttc atgttcaggt atcccaagca
     2701 atctccacct tcttgaaacc aattttacct taccgtataa ccaatcaaaa actgttagct
     2761 aaatttgtgc aggttgcagc aaacgaacag catgatgaaa aaaatccaca ttttggtaat
     2821 agccgccctg ctcccatctt ttattcaagc acaaagcgat acggttttca accgcaccga
     2881 tgagcaggga ttgaagcagg gcctctggcg aaaatactac tccaccgggg taaaagcata
     2941 cgagggttgg tttaaggata acaagcccac cggagagttt aagcgattcc acgaaaatgg
     3001 aagtttgaag gtgatactca gctaccgaca ggatagtacg gcctgggctg tgtttttcag
     3061 ccctaagggg aacaaaatcg ccgaggggaa attcgttgga caacagaagg atagcctttg
     3121 gcgctactac aacccggatg gcaccataaa ctacgacgag tactaccaaa acggcctaaa
     3181 aaacggacgt tgcaggcagt actatccgga tggtaagctg cttgaatctg ccccattcac
     3241 caacggtaag cttcatggag ctgttatcca gttctttcct gaaggcttaa ataagtcggt
     3301 agtcaacttc aacaatggaa ttcaggatgg cgacctgaag gtcttttacc cttccgggag
     3361 aaaacgtttg gagggaaaat atgccaatgg gctgaaagaa ggggaatgga tattctacgg
     3421 agaaaacggt aaaatcaccc agcggataag gtatgttaag ggtgtccccg aaaactacaa
     3481 cgagcttctc gaaagggaaa cccatttgct ggactcgctc atgcagaatg cgggtaggat
     3541 aaaagaaccc gattttgatg agatgtcata cggatctaac tacccctaat ccaactaccc
     3601 atgaagtaca gctttcgaat actactaatc atccagctgg ttggtttatc cagcgcagca
     3661 aaacctcaaa gcaagtactt tattggcttc accgataagg ccaactcccc atacaccatt
     3721 tcgaaccccg aacagttcct atctataagg tctattgagc gtagaacaag gcaaaatttg
     3781 gcattaacag aatccgattt acccgttaac cccagctacc ttgatacgct tgcaaatatg
     3841 ggtattacaa ttcatttccc gcttcggtgg atgaacgggg ttgtggctac aatttcaact
     3901 cccgaccagc tgacgcaaat tcagaatatt ccctttgtct ccggaattaa agagatatat
     3961 acaccaacca aggagcataa aagctcgaaa ttcacaatag aagaaccaca atcccaacca
     4021 ttttctaaac ctgacaagga cgcaccaaac tacgggatat cggccacaca ggtaaatatg
     4081 cttaacgggc atatactgca tggtaatggc tttttgggag aagggattga gattgccgta
     4141 cttgacgccg ggttttacaa cgttgactca catccggttt tcgacagcct ttggatgaac
     4201 aaacggatac tgggaacaaa agattttgtt aatcccacct ctaacatcta taacgaacat
     4261 gctcatggta tgatggtgct gtccatcatg gggggatatg ccaatggaca gcttgttggc
     4321 acagcacctc acgcccgcta ctggctcatc aggactgagg atgccagctc cgaacagctg
     4381 gttgaagagt acaactgggc ggcaggcgct gagtttgccg atagcgcagg tgccgacatc
     4441 attaacacct ccctgggata caccactttt gatgtgcctt ctcaaaacca tacctacagc
     4501 aaccttgacg ggcaaacgac agtgattacc cgggcagcta acatggcggc atcaaagggt
     4561 atgctggtgg tggtgagtgc cgggaacgat ggtacatcac catggtacta catttcagca
     4621 ccagccgact cgcccaaggt gctaaccgtt ggtgcagtgg atggcaatag gaacaaggct
     4681 gctttcagct cctttggccc aacagccgat gaacgtgtaa agccagactt atgcgcaatg
     4741 ggtgttggaa cgattattgc tactaccgat gggctggtgg gcagcggaaa cggcacgtcc
     4801 ttttccgctc cactaatctc gggcatgatg gcttgcctat ggcaggcaaa acctaaccta
     4861 acagtcagcg agctaatgga atcggtaatt tcagcctcaa gttcattcaa ttcccctaat
     4921 aatgaaatcg ggtacggcat tcctgacttt gccctggcca tgccggttgg aacctacagc
     4981 tacaagccaa acatcagaat cattccaaat ccgtttagca gccaagtgca aatatatatt
     5041 ccgctggaga ttgacgaagc tgtaagctgc cagctgctaa ccccgagcgg cctaacgctc
     5101 tatatgtgta ggcagattac cgaaaatggg gttttacgta ttaacattcc ggagagccta
     5161 ccaaaaggcc tctatatttt ccatatcgct gccggtaacc aagcgttcaa tttgaaagct
     5221 attaaagact aaccccgatg agcaacggca gtacccgctt aacccttctg gagctaaacc
     5281 tgctggtaaa agagactttg gccaagtcgc ttgccggtag ctactgggtg gttggcgaaa
     5341 ttagcgagat taccgaacat ccatccggcc actgctacat ggaacttgtg caaaagggag
     5401 atgacacgga taccataaga gcaaaagcaa gggcaaacat ttggggctac acctaccgaa
     5461 tgctgaaacc ctactttgag tcaaccacca atcggccact ttccagggga atgaaaattc
     5521 tggtcaacgc acaggttgta ttccatgagt actacggcta cagcctaacc gtactcgaca
     5581 ttgaacctga atacacgttg ggcgatatcg agctgaaacg tcgtgaggtt attgccaggt
     5641 tgatatccga tggcgtgatg aacatgaaca aggagctgga gctggacatg cttccccaac
     5701 gggttgcggt gatctcatcg gcaaatgctg cggggtttca ggattttgta aaacaactta
     5761 cccataaccc ccatggctac gcctttacct acgagttatt tccagcacta atgcagggcg
     5821 atgaagcaga gctgtctatc ataaacgccc tgggcaaaat ttacgacaag ctggcctgct
     5881 tcgacctcgt aaccattgtt cgcggtggtg gtgctcaaac cgacctctca tgcttcgact
     5941 cgtatacact tgcctcaaat attgcccagt ttcccattcc ggttatcaca ggaattgggc
     6001 acgataggga cgttacggtt gccgacatgg tttcccacac atcacttaaa acacctaccg
     6061 ctgtggcaga attccttatt gataaattcc gtgatgctga agctgttgca aacaaccttg
     6121 ccgggagtgt ttcccaactc gcaggaaaat tacttgccga catgaaggaa agcctaacca
     6181 gcaatcaagt agagctacaa gctattctaa accaaaaagt cgcccaaaac aagctatacc
     6241 ttcaacagct aaccagctca ctacctcata tggcaaaact agttcaggtg ctgcatgctg
     6301 atgagcttaa aagtttgaat ataaggcttt cgattcccat ttccagaata aaggccaact
     6361 atgttaagct aaacgcttat gctggtttgc tcaaaagcat gccgcataag ctactgcaaa
     6421 acgaaaaaat aaaaattgag caccacttta gcacactgaa gaacctgcac cccgacagca
     6481 tccttcatcg cggctttacg ataacctatt ccaacgggaa aattgtaaaa aatctgaaca
     6541 atttaaacgt caacgatgtg attaccactc aattttggaa tgggagcata gaaagtaaca
     6601 ttacaaaaat aaaagcaagc aaaaagttgc ttgctaacaa taaaaaatag taaatttaga
     6661 atttgatgtt taaagcagac aaccatccaa caaagctctg gcacttgaaa aaatttcgac
     6721 atatactatt acttgcttcg gttttttctt ttcataccgg caacatcctc cttgcacaaa
     6781 acagctcttc tgtgcatctg cttgacagcc tacttaccga ggccaaacaa acaaccggca
     6841 ttaggaagat aaccgcgctt gataaaattt caaggtacta ctgggagaaa aatcctgact
     6901 catcgctata ctactccaac ctggccttgg ttgaagccca acgagacaaa agcaacgaag
     6961 cgctgggcga tgcatacaac agtatgggta atgcttactc atcaaagcat gagaacgaaa
     7021 cggccattac ctactacctt aaatcgaaag actaccgtga gaagagcggt gactggacaa
     7081 aggtaggcca ctcattaagc aatctaggat tgacgctacg agatctcaag cgatacagcg
     7141 agtccatcag cagctacagg caggcagcaa aatattacag tgatgctaag aattacctct
     7201 ttgagggaat ttcatacctt gacattgccc aggtttaccg ccagctcaac gaccttaaca
     7261 gggctcttga aaacgccatt ttggcaataa atatttttct ggagcatgac atcactcaac
     7321 ccctggcccg atgctacaac tttattggaa acctgcataa agaccttaag aatttcgatt
     7381 tggcagagga ttactatctt aaagcttatg aaatttggtc aaaagcgaat aacctatccg
     7441 gtatttcatc tgctttaaac aacctgggaa taatttacga cgagaagggg aactacgaaa
     7501 aggcccttga aaactacaac cgctcgctgg atattgccat acaacttaac gattctatag
     7561 gtatttctac agcctacaac aatattgggt ttatatacgt gaagctcaac cagctggagc
     7621 gaggcatcaa cgcctacctg aaatctatgg ctatatcaag gcaaataggc gaaattgagt
     7681 cgtaccttaa tacttgcaat aatgttgcct cggtttacct taaagcagga atgctagaca
     7741 agtgtgagga gtacctgaat atagttcttg gcaatcttaa aagtataacc gagctggcct
     7801 acattcagga aacctaccag ctgctcagtg agctgtatgc caagcgtgga aactacaaaa
     7861 aggcattcga gtttaagtcg ctgcagctgg catacaacga cagcatatac aaccagcaac
     7921 aggtccgaaa catactcgaa actcaaacca ggttcgaaac tgaagcaaag gaacgtgaga
     7981 tacaactctt aaaaaaggac aacgaaataa agcagcttga gtttgagaag cagcgaaatt
     8041 ttcaaaacat tctaacgttc atcaccatcc tactggccat agcaattgcg gttaccatat
     8101 acatttcacg catcatcaga aaaaagaact tgctgcttgc cgaaaagaac agggcgctgg
     8161 aagaagcgaa taaaaaaatg gccgaaacag aagttaactt aagggagcta aatgccacca
     8221 aggacaagct attctctata atcgctcatg acctgaaaaa tcccttcaat gcgctgctgg
     8281 gatttagcga tatccttgag cgcaactaca acttcctctc cgatgaggag cgtattgagt
     8341 acattggagt tataaatgat tccacccaat cgctttataa gctgcttgat aacctgctgc
     8401 aatggaccag aactcagacc ggaacaataa cctacatacc cgaaaacttc aagcttcttc
     8461 ctctcgttaa gcaggagata accaacgtca acctgaactc cgagaagaaa aaaatcagca
     8521 ttaccgttaa cattgacgag aacattagcg tgtttgccga taagaacaca accgctacta
     8581 taatccgcaa cctgcttagc aacgccatta agttcaccga tttgggtggc cacatagaga
     8641 tagccgcacg ctataaggat caaatggttg aaacatctgt aagcgattcg ggaattggaa
     8701 taaacgataa tgaccttgac aggattttca tgcttgatgg ctcattcaca acaaaaggaa
     8761 catccaacga atcgggtacc ggtttgggac tactgctttg taaggagttt gttgaaaaaa
     8821 acaacggtaa aatctgggtg gaaagtgaaa agggaaaggg cagcaccttt ttcttcaccc
     8881 ttccagcaaa ataagctcac gcacacccct cctaatttta tcataatttt ttaatcagcc
     8941 aacccatttc tagtccagta taattttgta ttttttaccg atttttctga acacaaaaca
     9001 tgaagctggt tattcaaaaa tggggtgtag ccctactgct taccgcttac agccttatag
     9061 ctgctcaggc aggttcacca agcagcagta aggtggatag cctataccaa agagtgctgg
     9121 caatagccga cgaaaatcca caggaagcca gcaggcttgc caacctgtgc aggcaggaat
     9181 catctaaatc aggaaatagt cgtgataaag cgctatcgct catggcaaat gcccaggcct
     9241 taattgccat gtcgctttgg aacaacgcca gcgagaaaat tgattcggct tcgctttttg
     9301 tccaaaaact caacgatttg aatctcgaag ccgatttagt agatcttaga agcaacctgg
     9361 aggttaaggt ttcgggcagc gatgccgctc tcaaaatgct acaaaaagca tacagccaag
     9421 ctatcaaaaa taaaaggcct gacctgggtg taaagtttct cctgctccag gccgggcttt
     9481 accagcaaaa gggaaacctg gcagaggcaa acaggatgag ggaaattgcc cttgaccaat
     9541 ctagtaaagc tggttgcaaa aagtgtcagg ctgaatgcca gcaaaacatc gcaagctcat
     9601 actggcaaca aggacgcttc aacgaagcac tcgagagcta ctttaaatcg ctgattctct
     9661 tcgaggatat caacgatact atcgggatgc tagccgccct gaagggtgta ggcatatcct
     9721 accgagatct tggccagttt gagaaatcgc ttacctacct caacagagcc ttaaaactgt
     9781 cggatagtag taacaactat acggaagagg cagccatact caacattatt ggaagtctgt
     9841 attttaaatt caaccaggtt gacgacgcac taaagcaata cgaacaaagc ctgcaaataa
     9901 ggaaaaaact gggattactt cggtcggcca tttcaacgca tagcaatatg gcccgtgcct
     9961 acttgcaaaa atcaatgtat aaacaggcca tcgaacacct caatgaagct ttggcccttc
    10021 aggaacaagt tgttgacccg ctggcagagg catcaaccct aacagaaata ggtaacctta
    10081 acctgcagca gggcaatata gccgaagcgc tgcgtcacta cctaatggcg ttaaaacttc
    10141 ggcaaaccta cgggcaggat gaggatattg ccaaatcgct caccagcatt ggccttacct
    10201 accgaaggtt gggaatgtat aaaagcgccc ttaaatacct tgaacaggca agggaaataa
    10261 ttcaggacat gaagggtagc atgaatgatg ctgcatacat cctacaaaac ctggggcata
    10321 tctaccttga cctcaactcc cccgacaagg caattgccac atataaggaa gccctgactc
    10381 ttaaagaaaa aagcggcgat agcataggcg ctgctaaaat tttaaagaac attgcccagg
    10441 cccagcttaa aagcagccaa cccgaccaag ctcgtacatc aatttcacaa gccctcaagt
    10501 tggtaacccg aacacatagc accagcgata ttgcagatct atacaacgag ctgggtaatg
    10561 ttgaacgaca ggctaaaaat cctggcatgg caatcaagca ttaccacaca gccattgaaa
    10621 actataaagc tcttaataac aacgagggag ttgccctttg cctaagaaaa atcggagaaa
    10681 ttcaggttgg gcaaaaacag ctggatgagg caagaaaaaa catcaacagc tcaattgaaa
    10741 ttgggaaaca atcagacaac aaataccttc tatcatacgg ctatctagct caaagtgagc
    10801 tataccgcgc acaaggcctt ttcgataaag ccctggaaag ctacataaag catataggca
    10861 ttcgcgacag cctagagagc gttaaaagaa acgaggcgaa cctagaggct cagatagacc
    10921 tggagctcga ccaaacaaaa accgagatta aggttatgga agcagaggtg gaagcgctca
    10981 gacagaagac tgctctagat aaagccataa tcgagaagca aagattattc cgcaactcgc
    11041 taattgccat aatcctactt gtcgtaactg cggccagcat agctttctac agccttattc
    11101 agaaacggcg ttatgccaag ctacttgagg agaaaattga ggaaatcagg ctggtaaacg
    11161 aaaggctcaa cgagtctgag agcgagctgc gtcaaaccat taaaactaag gataagctat
    11221 tttcaatcat tgcccatgac ttgcggagcc catttaccgc cttagttgga ctatcggagg
    11281 taatggcaac aaacctggag agcctttcgc ccgatgagat tcgtgagttc agccaccaca
    11341 tccaccgctc ggcaaccgag gtgctcaacc tgactgagaa ccttctaagc tgggcaagaa
    11401 gccaaaccgg aaaaattgag ctctcgccaa aaccaattaa tctcaaggac cttatcaacc
    11461 aggttttttc ggtagctgcg attcctgcac aggaaaagaa aatcaaactt cgggaaagca
    11521 ttcccgccaa cctaacggtt tttgccgatt tcgataccct ccaaaccgtt atcaggaatt
    11581 tggtgagcaa cgccattaaa ttcaccccgc agggtggatc aatcggaata tcagcaaaaa
    11641 attcaagtaa cagcatggtt gaaatcgcta ttaccgatac aggcgttggc attgatcctg
    11701 agaatcaggc caaacttttc agggttgacg ggttcaccac caagggtact aatcaggaaa
    11761 acggaacagg attggggcta atactctgta aggaattcgt tgagttgaac catggaacta
    11821 ttgaggtaag cagcacaccg ggacttggaa caacattcac catttcatta ccctcaaata
    11881 attagaacta tgaccaaaaa gccacaaagg tatattgatg ccattaacga aattgagcaa
    11941 atcatcacag aaattgaaaa caacgagctg gacgtggacg acctgaccga aaagataaga
    12001 agggcctctg agctgctcaa attctgcaaa accaagctcc gggctaccga ggaggaaata
    12061 caaaaaatca taagcgattt tgaagaggat agctaaaaga gctgcccaag cctcattcgc
    12121 cctttatgac tgttggacaa tgtgtacccc ttcatcaaca tctacaaata gttctatcaa
    12181 aaaattgtta atgatatttt tacccttctc tctgtcctaa actactccta taaacttcca
    12241 ggcaaaaccc tgaagaatat ttcaaaaatt taactttatt tgcatatagg ttgaatagag
    12301 cattatggct tacagcatag ttgcaatttg tgggtctgaa aggctctccc gtgaaattaa
    12361 gagcatcgtg ggaattgccg aatttgattt tgatttgcac ttcgttaccg acgacacgaa
    12421 tatcatcacc aacctaacgc agctcaaccc cgatattgtt ctttttgatg tttcctattc
    12481 ggagtatgca gcgccagatc tgctcaccat tattaaaaac gatataaaaa ccgaatcggt
    12541 acccatactg gccatagccg atttcttaca acctgagaga atccaagccc ttttcattgc
    12601 cggtgcagac gactttatta gccagcctct tgctgctgct gagcttattc aacggaccat
    12661 gataggcatt caacggggaa gaatgctgaa aaaactaaaa gagcaatccc ggcagttcaa
    12721 cagcattact gatgccatta gcaaggctgg taactccatg attgttatca accacctggg
    12781 taaaattacc tgggttaacg aagggtttaa acggctatac gaatgcccgc tcgactactt
    12841 tgtatccatt ttcggtgaca acctatttga ttcctcaaca aacccggtta cacgagaggc
    12901 acttgacaaa tgccggaaaa atggcgagta tgttgtttat gagagcaaat gggttacccc
    12961 tcagggcaag gagaaattca ttcaaacatc gcttacgccg atttttgata gcctccagaa
    13021 cttaagccaa ataatcgcca tcgaaaccga catctccgac ctgaaagcca ttgaaaaaga
    13081 cctggaggaa aagcatgaac acctgcttac catcaccgaa aacctgaaga gcgttaacgc
    13141 cgtgctggca gagcatcagg aaagaattca gaaacaagcc ctacagctcg aggaggagaa
    13201 acaaaaatct gaaaatttac tgaaaaacat ccttccatgg gaggttgcat atgcccttca
    13261 gaaaaaggga tcatacaaac ctaagaagtt caaggaggtt tcggtaatgt tcaccgattt
    13321 tgttgggttc tcaaaaatca gcagcaacta tggtgatata gacgagttcc tcagtgtttt
    13381 gggtggtttc tttgaggtat tcgacgagat aacctcccag cgtttcatcg agaaaattaa
    13441 aacaattggc gatagctata tgtgtgtagg aggactcccc agagtaaacc gtagccaccc
    13501 attcgacact gtacttgctg ccattgagat tcagcgattt attcatgata aggctgtaca
    13561 ggctgcaatg gaaggtaaac cggtatggaa cctccgtatt ggcattcaca ccggaagtgt
    13621 aattgcagga gtaatcggaa aacgcaaatt cgcctatgat atttggggcg acacggtgaa
    13681 catagccagc cgaatggaga ctgccagcga acccggaaaa gttaatattt cgcaaaccac
    13741 atattcctac attaaggatt acttcgtttg cacttcgcgg ggaaaggttc ccgcaaaaaa
    13801 cctgggtgaa attgaaatgt actttgtgga gcgcataaaa cccgagtact ccgaggatga
    13861 acttgggcta atacccaact cctccttcag gaaaatagtg gcctccttct aaccttcagc
    13921 aaaggcctta cttccgcttc atcccattgg agagtgaaaa actaaaggga agcagctgct
    13981 ctacatcctc aaccctgaca atttcgttac tcccaaccat tagaatggca aacggtttcc
    14041 ctgaaactgc cctgtactcg ctcataacct gccggcatgc tccgcatggg gttactggat
    14101 tctccgtaag tccatctccc gaacttgcca caacagccat tgaaactaca ggaattcccg
    14161 gatatcgagc accggcatag aacaacgcaa ccctctctgc acatagcccc gaagggtaag
    14221 caccattctc ctggttgctg cccgtgataa tctccccatt ctcaagtagc acggctgccc
    14281 ctacccgaaa gtgtgagtag ggtgagtagc tcgacgacag ggcagcaaca gccctgtcaa
    14341 ccagttctct ttcgttggga gctaactctt ctttgcccct caaccggtta taccgaaatt
    14401 gaaagctatt actttcgctc atggaatgca aattattaga aaacctacat ttaccccaca
    14461 aatgtagaat aaaaaactat tgactattta ctttaaaagg aaaactaaac atgcctggag
    14521 aaaattgtac acaatcgcct tagcattaag cagtcaagta tcaaaatccc ccatcagctc
    14581 caggaattta gagtttcatt ttgcccggtt ttatcatatt ctcaggtgct aaaatccgat
    14641 cgagctgctc ccgtgtaagc aacccttttt ccagcaccag ctggtaaaca cttcggttag
    14701 atgaaagcgc ttcttttgca agctgggtag aggcctcata gccaatatag gggttaagag
    14761 cagtaaccaa cccaatactg ttgtgaacca tgctcctgca ccgttcctcg ttggcagtaa
    14821 tcccatctat acagcgatat tttagcgtat tcataccatt tttcagcatt tcgaccgatt
    14881 caaaaatgct gtgcacgatg accggttcaa aaacattcag ctcgagttgt ccgccctcgg
    14941 cagccatggt aacggttaaa tcattgccta tcaccctaaa agcaatctga ttaaccactt
    15001 cgggaatgac ggggttaact tttccgggca taatagacga accgggttgc atggggggaa
    15061 ggttaatctc gcttaacccg gttcttggtc ccgatgataa cagccgaaga tcgttacaaa
    15121 tttttgaaag cttaacagcc agccttttca cagcagagga gtacatcaca aatgcacctg
    15181 tatcctgagt tgcctcaacc aggtttgcgg ccaaaaccag ctccagcccc gtaatgctcc
    15241 taaggtgctt cagcactttt tgcgggtact ccggatcgga gttaatgccg gtgccaatag
    15301 cagttgcgcc catgtttatt tcaagaaata gttttgcgtt ctgtgttagc cgttcaattt
    15361 cctcccccag ggttacggca taggcttcga attcctgccc taaagtcatg gggacagcat
    15421 cctgaagctg ggttcgcccc attttgatga cgtgggcaaa ctcttgtccc ttcttacgga
    15481 acgactcgat gagggattca agcactttaa tcagcttggc gttggagcac actatagcca
    15541 gcttaaccgc cgtggggtag gcatcgttgg ttgactgcga caggttaaca tgattcaacg
    15601 ggtggcaatg ctggtactca cctttggagt agcccattaa ttcgagtgcc ctgttggcaa
    15661 tcacctcgtt agcattcata ttggttgatg tgcccgcccc tccctgtatc atatcaacca
    15721 caaaatgatt gtgcagctta ccgttagtaa tttccttaca ggcagcaaca atggcatccc
    15781 taaccttttc gtcaataagt cccagctcaa agttggcctc ggcagcagcc tgtttaacat
    15841 tggccagaga atcaatcaga tcagggtaaa agttcagggt tacccccgaa atgttaaagt
    15901 tttcgagggc acgaagggtt tgcactccat agtaaaactc cgcagggaca tcccgttcgc
    15961 ctagcaggtc atgctcaacc ctggtgctgc ccgattcata ctgcgaagct gcatctacca
    16021 tccggttgtt ggcattgtgc atacgacgtt gtattacacg cgttatgttg atgagaattt
    16081 tcaacccgat ttccgcatgc tggctaagca cattatcctt cagcacgaaa acttttgttg
    16141 gttttagggc cctggctgag gtggagtgag gcgaattgct tgcccaagaa ccctcgccaa
    16201 gaaaatcgcc gggggtaaag taggtaagtt taagctccac cccaaagggg ttgctcctaa
    16261 aaagctccac ttcgccctca taaattacca tgatccccac ccgctggcta ttctccctaa
    16321 acagcaattc gccaggctta aactcaatca cctccatcat gttaactaag ctttcaagtt
    16381 ccttttcgtt aaggccccgg aaaagctcaa tcttttttaa aaattctatc atctggttgg
    16441 tattcattgt gctataattt aaaatttaca aaggtgtttt accacgaatt agtgcaattt
    16501 actaaatgtt agctaataaa acaggaaaag cagaaattcg ttcagctgaa ttatactgca
    16561 aaagccatta atacccgttg tacttaatga gaaacggata tttttctacc ttataggagg
    16621 aaaaaaggca tcccttacgg gacgccttta gcaagcactc accaaccaac taaccattaa
    16681 tcgataagac cataaccaac cttttgttct aaagaagaga tttgtgcctg ctctgtgatg
    16741 gcctgctcgt tgaaatagga aagtatccgg tcgagcataa tggcctttga tgagcctgta
    16801 gccgtgtact tgtaaagatt agatgccaac agccgtattt ggttttggtt ggcaattagc
    16861 acctgaaccg ggtcaatcgt atcaattttc agcttcccct ccagctttct taactcctca
    16921 atgtttgaaa caatgcacct gtttagctcc atgtacgact taaatgcggg agtgtttctt
    16981 cgtgagatta ttgctataac ctgcatgcta tcggggtcaa taaccggcga accgctattt
    17041 ccaaagcaag taagcccatc cagttcaaga catctaatcc catttttact tgaaaatgcc
    17101 gaagaaataa cagcggattt tatcgacagg ttgttttcac caaagttgaa acccaatgta
    17161 gccacttgtt ttccaatttg aaaatttcta cactcgcaca gggttaaacc gggtattttt
    17221 tcaaattcag aaaaatttat gttgaaaact gcgtaatcgg actggttatt ggtgtagcca
    17281 attcgtaaat cgttaataaa ttcgttgtag ccaatcctca tggttgccgt tggagtattg
    17341 gcatcgcctt caacaaagga aatctccacc ttttgggcat tgtttatgta aaatgcgtac
    17401 tcgctggtta tcaacgagct gttaaccttg aatccggaaa gagaatcaat aaccattccc
    17461 ctttcattaa tgaagtgaag gctacaaact gatgcgtggc attgttccca aacttgtgat
    17521 agcataaccg taagtttttt tagtctgtta acatcaaagt gttagcgcct taataagcgc
    17581 atatacaaaa agcagggtta ccaaaaaaaa gatggtaaac accccggcat catccttaaa
    17641 cttcagcgtt attttctccc ttatggaatt ttccttcttc ataacttatc tttgcttaaa
    17701 cactaaccct ggtttactcc ccgcaagtac atttcatttt gattggtttg cttaccccca
    17761 cttattacat atgaaatgtt tgccagttca aatttacccc atttcagatt accaggaaaa
    17821 gtgtcaaagc tgtcaagttt tagaaatttc cagatttgct atctaaacaa tcacatatct
    17881 ttgcatagtt aacggtattt atacatttca gttagcttaa taaacatagt accataccat
    17941 ggttgagctg ctgactcagg aggttgaaag taggtttggc aggaaaataa gctgccagaa
    18001 agactgtatc tccctctcta tcagtataat ggatacatgt agggagtatc tgagcccggc
    18061 aaccctacga aggctgttcg gtcttttagc tacgaattct aatccatcgc atgtaaccct
    18121 tgatattctc tcgcgatatg caggatatac gggttgggat gattttgtca gtcacaaaaa
    18181 gagcaaaatt cctgaaggaa tcattcggtc agctgatgaa gattgggagc aagccgctga
    18241 acatgccaaa gcggtcagca gtaaaacact caaccatatc atgcatttat caggcattag
    18301 gtttgaagag acagtccacc gacggcaggt ggaagagcgc cttgaccaat ttttgaacag
    18361 caacaaccct gctactgcta ttatagctcc cggaggctac ggtaaatcaa tcctgctcgc
    18421 ccattggtac cagatacaca gagatttaaa ggctaacatg aacgacgtat tcctactggc
    18481 accggcgacc atgctcgacc attacgcaag ctccaacact tttatcggcc attggctggc
    18541 acagcttctt gggcaaaaat cgttagaaag cctacttgta aaagatcttc ctggtagaat
    18601 agtcttcatc tttgatgctc tggacgaggt agtgtcggta ggagccaagt tcaacaagat
    18661 actcgaagct cttgccgacc tggctgaggc tttcgcccaa acaaacaggt ttaagctggt
    18721 tatttcgacc cgactgtcaa cttggactca gctaaagcca tacattacgc acccactgct
    18781 ttggtatttc tccgattctg agctgctaac ccacgaagga gccaatattc ctccgctttc
    18841 gcccgacgaa gttcagcaaa tccttgacca aaccataaac ctaagattca accagcgttt
    18901 gcttattttt gaacttcatc c
//
LOCUS       DAOPHX010000037        18892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_3194_length_18892_cov_5.166162, whole genome shotgun sequence.
ACCESSION   DAOPHX010000037 DAOPHX010000000
VERSION     DAOPHX010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 18892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18892
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3194_length_18892_cov_5.166162"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="PK502_04315"
     CDS             <1..991
                     /locus_tag="PK502_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPH00073.1"
                     /translation="VEVMLGLTDYYFPIFEEILDKYQLPQELRFLPVIESALNPKAVS
                     RAGATGLWQFMYGTGRRYNLTVNSYVDERRDPIASTHAAARFLRDLYAIYGDWTLVIA
                     AYNCGPGNVNKAIRRSGGKRNYWDIYYYLPRETRGYVPAFIAASYTYYFSHEHNLVPQ
                     PINIPPTTDTIVINDMLHLQQVSEVLDIPLDMLRDLNPQYKLDVIPGKFKPYALRLPL
                     DGVGPFIDREKDIYAYQDSLFFNPRVTISPNKYTSSSQYDIPSGSIRYVYRVKEGDVL
                     GIIAEKYGVSVGQLRSWNNIRGNIIRIGQRLVIYIPERTANKMGISGDKSQTS"
     gene            1089..1673
                     /locus_tag="PK502_04320"
     CDS             1089..1673
                     /locus_tag="PK502_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00074.1"
                     /translation="MAHNRNKAALSIALSFLLFVTTACYSGKKTADYSTLTNNAFAFV
                     EYYQTTDVQVMAGEYHGGRRIDSPTYAFNPEDRTLNIYRYPDGLQPDSAIVVLGVGKI
                     LKNAAGSGVSSFLVGANQLPFSYNDLTITSLSNNKLFLTYQNKSISLKAGEEWKHYTE
                     KTDTLKLQGLPVIKTMQTSTIRFHGFLSRNILKK"
     gene            1750..4155
                     /locus_tag="PK502_04325"
     CDS             1750..4155
                     /locus_tag="PK502_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HPH00075.1"
                     /translation="MIKHLQSIRKLAFTAIIGVMTLTGYAVPKHNLPITITQPDGTKI
                     SCFVSGDEFHNWLHDAAGYTIMRNPANGFYVYAVEKNGKLTSSDYVVGKVNPRNSKDL
                     KPYVNISRNEYLAKRHRKQTDNHHVNLDITKGTSGIAPIRAVKGVTMNNIVVFIRFKD
                     DVVADENISYYENVYNGSSASVANYYSTVSYGKFSVSSTFYPQLNGTLVTWYVDENNR
                     NYYQPYDATTNPNGYNPDASNYNDPTSSTYREHTLLKKAVEAVGASIPTGINLDINGD
                     NYIDVISFILAGSNDSWNDLLWPHQWSLWSQSAFINGKQVGDYTLQLQYFGGSQIDLG
                     TLCHEMFHAVGAPDLYHYDDDLNLSPVGYWDIMEGTLDYPQNMGAHMKYLYGGWIDQI
                     PEITTSGTYTLSPLATSPTGNSYKIPSPNSYSEYFVVEYRKKESYDSDLPGNGLLVYR
                     INSKLEGVGNADGPPDEVYIYRPNGTPTLNGSINSAHFNSSVGRTSINDNTNPSSFLT
                     DGTNGGLKISNITAAGETISFDATIDFIPYVVLKNDRGYATAIGDESTTLTVATRFTT
                     QDLASLVGRKIKMVDFYIRGEDGTDVTTNETVKVWEGGTYGNPGSLIYSKNISSEVVP
                     DQWTSHTLSQPIVIQPNKEYWVGYTATASGGYPFATDGGPMVAGKGGWIYIDNQWYEL
                     ININSQLNYNFLVRAVVGSETSDAETLTADAVNFKVFPNPANANSRVSITLPMAGRVA
                     VDLYNMLGQRVGGSMPTHYEAGVQSLLLSGNELSNGIYLLTVTYDSGTHHTVETQKIV
                     IAR"
     gene            complement(4389..5735)
                     /locus_tag="PK502_04330"
     CDS             complement(4389..5735)
                     /locus_tag="PK502_04330"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPH00076.1"
                     /translation="MNQLKVDLSNIYTQVAKEEVFALKTKIAQNQLLLEQGTGKGSDF
                     LGWLNLPSSITKEQLYDIQHTASELRAKSDVVVIVGIGGSYLGAKAAIDSLSSSFTQL
                     TEDVLPKIIFAGNSICEDYHAELLELLETKRYSIVVISKSGTTTEPAITFRVLKQHLE
                     RQVGKTEAASRIVAITDLSKGALRKLADSEGYRTFVIPDDVGGRFSVLTPVGLLPIAI
                     AGIDIVKLVEGARKAMDDTSSSVAFDQNPACIYAAARNALYQKGKKVEILVNFTPRLH
                     YLTEWWKQLFGESEGKEGKGIFPAGVDFTTDLHSMGQYIQEGERMLFETVLSVEKPSH
                     RLVVPDDPDNLDQLNFLSGKRFSEVNLMAQMGTIAAHSDGGVPCISISIPELSPYWVG
                     YLFYFFERACALSGYTLGVNPFDQPGVEAYKRNMFALLGKPGYEADGRKLREKLGL"
     gene            complement(5765..6778)
                     /locus_tag="PK502_04335"
     CDS             complement(5765..6778)
                     /locus_tag="PK502_04335"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPH00077.1"
                     /translation="MSTNTEIEKKPSIAVVGSGSWATALAKVLHETQDSIGWCLRDPE
                     LIGYIKKYRHNPNYLSAVEFDVKKLYLSVDINEVAKLYDILLFVIPSTYFQAWLEPLS
                     VSLEGKFIISAIKGIVPGMDCTIAEFFNQQYGVPFDRIGIVSGPTHAEEVALERLSYL
                     TISCKDKLQAKHVASYFETHYIKTNIGTDIYGTEYSAVLKNIYAVASGIAHGLNYGDN
                     FLAVLISNAQREIERFLKKTYPSKRRLSTSVYLGDLLVTCYSQFSRNRTFGTMIGKGY
                     SVKSAMLEMSMVAEGYNAAKCIWEINKKYNVKMPIANAVYNILYENISPAIEMKLLTA
                     KLR"
     gene            7121..8152
                     /gene="rlmN"
                     /locus_tag="PK502_04340"
     CDS             7121..8152
                     /gene="rlmN"
                     /locus_tag="PK502_04340"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPH00078.1"
                     /translation="MEKQNNKVPLIGFTPQELCSTMQEYGGTSFQAESLMRWIYRKRE
                     KDISTFSDIPKAILQSVEQSYTVGLYPPKESKRSDDGSVKYLFAIGQNQVFEAVAMSS
                     GNRHTLCLSTQSGCRMGCRFCRTGSIRFNGNLSAGDIINQVLSIPDSKRLNRIVLMGM
                     GEPLDNLKVVNKTLEILTADWGCALGKSNITISTVGLLSELEAFLKSPGCNLAISLHS
                     PFPDERAMLMPVERTNPILEIIKLLKHYPLKKPLRLTFEYVALGSLNLSKNHARAIAT
                     LLSGLSCHLNIIPWNRHNAATFQSPTSDELTNFMELLNDAGIPTNLRKSMGGDIDAAC
                     GQMAGNAPH"
     gene            complement(8200..9324)
                     /locus_tag="PK502_04345"
     CDS             complement(8200..9324)
                     /locus_tag="PK502_04345"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPH00079.1"
                     /translation="MNFSELNLNPHILEGISTMGYGNPTPIQEQAIPVILQGRDLIAC
                     AQTGTGKTAAFLLPILGKIAEKPTTEGYINTLIIVPTRELALQIDQQVEGFAYFISTS
                     SIAVYGGNDGNSWDRQRMALEQGADIIVATPGRLLQHIQMGYVKLDRLEHLILDEADR
                     MLDMGFFDDITAIISQLPTRRQTLMFSATMPPKIRQLAKTILDNPVEISLSVSKPVES
                     ILQAVYMVEDSEKIGLLQSLVAGKEYVQSVLVFASKKMEVKDIERKLVRESIMARAIH
                     SDLEQSQREQVLRDFRNRKFQVLVATDIVARGIDIEDIDLVVNYNVPADPEDYVHRIG
                     RTARAQSDGVAITFVNKKERPQLARIEKFLGETIFRVSHS"
     gene            9658..10374
                     /locus_tag="PK502_04350"
     CDS             9658..10374
                     /locus_tag="PK502_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HPH00080.1"
                     /translation="MRIKFFSISNRYRYVFIDLDRTLWDFERNSLEALHELFSEYKLD
                     SVVADATTFSRTYVKHNNYLWNSYCKGELTKEVLRYKRFDATLSELGVNNHQLAAKLG
                     EEYLRILPLKTILIPNAKELLDYLAPKYGLMILSNGFHEVQVDKLKRCGLNHYFDRVV
                     TSELSGYHKPDPRAFGYALSSANIRKNQGIMVGDDLEVDIVGARKFGMDQIFFNPERK
                     PHGEKVTYEVETLKEITQIL"
     gene            complement(10495..11973)
                     /gene="dnaB"
                     /locus_tag="PK502_04355"
     CDS             complement(10495..11973)
                     /gene="dnaB"
                     /locus_tag="PK502_04355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPH00081.1"
                     /translation="MGKVPPQAIELEEAVLGAIMVEKDAVVIVSEILKPECFYKETHQ
                     EIFKAIQDLSSRLEPIDLFTVTEELRRQNKLEEIGGAAFLAQLTTKVGSAAHLEYHAK
                     IIAQKFIQRELIRVSTDIQNRSFDEDVDVDELLDYSEMELFKVAEGNIKSDTQPITTI
                     IKKAIKQIEEAGKREDGLSGVPSGFTALDRLTSGWQNSDLIIVAARPSMGKTAFVLSM
                     ARNMAVDHDVPVAFFSLEMSNLQLVNRLIVSESGIASEKIRNGRIDQSEWTQLTVKIK
                     NLSEAKIFIDDTPALSLFEFRAKCRRLKAQYDVGVIIIDYLQLMTGPSETKGNREQEV
                     STISRSLKAIAKELNVPIIALSQLNRSVETRGGYKRPQLSDLRESGAIEQDADVVIFI
                     HRPEYYGFMEDEDGNSTQGIAEIIVAKHRNGPVDDIKLNFRKEMAKFSDFDDVHYGDI
                     QPSHSPFTSSVPVTFSSKMNEDTHGDYDFLSGQGVDSSDIPF"
     gene            12231..13241
                     /locus_tag="PK502_04360"
     CDS             12231..13241
                     /locus_tag="PK502_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00082.1"
                     /translation="MNRVILFSLTALVLLISCKPNRTERIDPTNVPASIQLERFEQDL
                     FSASPDSLQTLVPELRKKYGRFVDLFSEGIIHIGKTDSPEYCNYLSQFLSDTMVQNAY
                     RLSQQKFQNISSIKVCLNEAFNRFHCYFPDKPVPKIYTFVSGYNLSLAVDDSILAIGL
                     DRYLGSNTVQYKLLGIPRYQVRNMRPEKIPSDAIRAWMYGSFSFHDSTDNLLSNLIYE
                     GQVMYLTKQMLPQESDSLIFGFTPSQHRWCLKNEKAMWTNLIENKLLFSTNTLDINKF
                     INDAPFTSGFPQESPGRAAVWIGYRIVESLMKNNDTLTLSEMMGISDYQKFLAMSRYK
                     PR"
     gene            13530..15200
                     /locus_tag="PK502_04365"
     CDS             13530..15200
                     /locus_tag="PK502_04365"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPH00083.1"
                     /translation="MKSDIEIAHEVKMKPVTEIAGKLGIDADDLVLYGKYKAKVPLKY
                     IDKNKYQKNKLVLVSAISPTPAGEGKTTVSIGLAQALNRLGKQTTVVLREPSLGPVFG
                     VKGGATGGGYSQVLPMEDINLHFTGDFAAIERAHNLLAALIDNNIQSKKRSLGIDPRT
                     VRWKRIMDMNDRSLRHIIVGLGGTSHGVPRETGFDITAASEVMAILCLAENIDDLKKR
                     LGNIFIGFTYDKKPIYARDLNAQGAMAALLRDAIMPNLVQTIEGTPAIIHGGPFANIA
                     QGTNSVLATKLGLSLSDFVVTEAGFGFDLGAEKFIDIKCEYAGLNPNTVVLVATVRAL
                     KYHGNKPLKEVSQPDLEALKRGIGNLQKHVENMKQFGICPVVAINRFTSDTDEEVEYI
                     KQVCRDMSVQVAAVDVWGKGGEGAIELAEIVNKIASSCSAQHKTLYDWSWTPELKIET
                     LAKRIYGAEAVDYTPQAKADLEKVYSLGLDKLPICVAKTQKSLSDNPDLLGRPKDFVV
                     TVREIEVASGAGFIIPITGQIMRMPGLPDEPAAERMDVDNNGVITGLF"
     gene            complement(15266..16384)
                     /locus_tag="PK502_04370"
     CDS             complement(15266..16384)
                     /locus_tag="PK502_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00084.1"
                     /translation="MMKGWFILTILLLLTFPKVFGQQVADTLRLNNQELSANKPNDIS
                     VSTQIFLGYRYFIGNSGSTNEFTVKRGYINFIKSLNKNISGRITPDITLDREGDGQGD
                     MEMRLKYCYMKLEEPGDFWIFTNPAVFIGEVFTPWIEFEEKINPYRIQGNHFLEKSDI
                     LISADFGVMAAALLGGTLNEGYQTRVGSDYPGRYGSLAIGVYNGGGYHNIEENNNKTL
                     QYRLTLRPLPDMIPGLQLSYTGATGKGNTTLSPDWNTNVGVLSYQGQPVTLCGQVFSS
                     VGNYSGSLADTLGVSYKVWGWSAFVEFKLLRNRLSIFGRYDYVKGRNLSEFGNITQSV
                     VGVAYSLFRRNKLVVDYSRNESSGKVEEVIEFMVELAF"
     gene            16499..17155
                     /gene="fsa"
                     /locus_tag="PK502_04375"
     CDS             16499..17155
                     /gene="fsa"
                     /locus_tag="PK502_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HPH00085.1"
                     /translation="MKFFIDTANLDQIREANDLGVLDGVTTNPSLMAKENIRGSENIK
                     KHYVDICHIVKGDVSAEVIATDYEGMIREGKELAALHPQIVVKVPCIREGIKAIKHFS
                     DSGIRTNCTLVFSAGQALLAAKAGATYVSPFIGRLDDTSTDGVALIRQIVEIYRHYGY
                     QTKVLAASIRHTMHIIQCLEAGADVATCPLNAILGLLNHPLTDIGLQKFLDDFKKVNG
                     "
     gene            17155..17778
                     /locus_tag="PK502_04380"
     CDS             17155..17778
                     /locus_tag="PK502_04380"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPH00086.1"
                     /translation="MPECLYIKMYPQNLNPRDIERAVEVLKRGGVIIYPTDTVYGIGC
                     DITNTKAVERIIKIKGMKPKEANFSFICHDLSHIADFARVDNSTFRLMKKNLPGPFTF
                     ILPGLNRVPDYFLSRRKTVGIRIPNHPIPVEIVRQLGNPILTTSLKDSDEVIEYTTDP
                     ELIFENYQNLVDLVIDGGAGNNIPSTVVDCTGDEPAIIREGLGELVF"
     gene            18220..18450
                     /locus_tag="PK502_04385"
     CDS             18220..18450
                     /locus_tag="PK502_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00087.1"
                     /translation="MVEETLWDGFDHIMSELHRACVTNHMDLPPTPSAKGACYNINLP
                     ADLNNLPAKESLGSVLSRQKQAKIKESLSGSE"
     gene            complement(18667..>18892)
                     /locus_tag="PK502_04390"
     CDS             complement(18667..>18892)
                     /locus_tag="PK502_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HPH00088.1"
                     /translation="IPEAANMIFDLRENFGVFCSMVVYPVVPKGQIMLRLIPTAAHTL
                     NDVKETVEAFRVVRDRLAAGKYNKEKIAQF"
BASE COUNT         5337 a         4592 c         4134 g         4829 t
ORIGIN      
        1 ggttgaggtg atgctaggac taaccgacta ctacttcccc atatttgagg agatactcga
       61 taagtaccag ctgccacagg agctaaggtt tttaccggta atagaatcgg cgttaaaccc
      121 caaagctgtt tctcgtgccg gcgccacagg attgtggcag ttcatgtatg gaacaggtcg
      181 aaggtataac ctaaccgtta actcttacgt tgacgagcgt cgtgacccaa ttgcctcaac
      241 gcacgctgca gcacgatttc tgagagatct ctacgccatt tatggagact ggacattggt
      301 gattgccgcc tacaactgtg gtccaggcaa cgtgaataag gcaattcggc gctcgggcgg
      361 caagcgcaac tactgggaca tatactacta cctaccaaga gaaacacgcg gctacgtccc
      421 ggcctttata gccgccagct acacctacta ttttagccac gagcataacc tggttcccca
      481 gcctattaac atacccccta ctaccgatac cattgtgatt aacgacatgc ttcacctcca
      541 acaggtttcc gaggtgctgg atatcccttt ggatatgctc cgcgacctta acccccagta
      601 caagctcgac gttattcccg gcaagttcaa gccttacgcc cttaggctgc cgctcgacgg
      661 agtaggacct tttattgaca gagaaaaaga tatctatgcc tatcaggaca gcctgttttt
      721 taacccacgg gtaaccatta gcccaaataa atatacatca tcctcccagt acgatatacc
      781 atcgggctca atccggtatg tttacagggt aaaagaaggc gatgtattag ggataattgc
      841 cgaaaaatac ggtgtatccg tgggtcagct aaggtcatgg aacaacatta ggggcaacat
      901 cattcgcatt ggacagcgcc tggttatcta cattcctgaa cgtactgcaa ataaaatggg
      961 gattagtggc gacaagagcc aaacctcata gagttttcac tctagcatga attgacacat
     1021 tcatagaaag tatgacctta ccagcataag gcagatgggt tttgcaaaac acctacaagc
     1081 aattcgctat ggcacacaat cgtaataaag cagcactttc tattgctctt tcttttttat
     1141 tatttgtaac taccgcatgc tattccggta aaaaaacagc cgattattca accttaacca
     1201 acaatgcttt tgcctttgtt gagtactacc aaacaacaga tgttcaggta atggcaggtg
     1261 agtatcatgg cggcaggcga attgattcgc caacctacgc attcaacccc gaggacagaa
     1321 cacttaacat ataccgttac cccgacgggc tgcaacccga ttccgctata gttgttcttg
     1381 gggtgggcaa aatcttaaag aatgctgccg gaagcggggt ttcctcattc ctcgtagggg
     1441 ctaaccaact ccccttttct tacaacgacc tgacaataac cagcctgtcg aacaacaagc
     1501 ttttcctaac ctatcaaaac aaatcaattt cccttaaagc cggggaggag tggaaacact
     1561 ataccgagaa aaccgacacc ctaaagctac agggcttacc cgttattaaa actatgcaaa
     1621 ccagcacaat acgctttcac ggattcctgt cgaggaatat cttaaaaaaa taaaggtatt
     1681 ccaatgcact agtttacact taaaactgga agttttatat ctaaattaac aataaaattg
     1741 cttaatatca tgataaaaca cttacaaagt attaggaagt tggcatttac ggccattatt
     1801 ggagtaatga cccttacagg gtatgccgtt cctaagcata atttacctat aaccatcacc
     1861 cagcccgacg gcacaaaaat cagctgcttt gtcagcggcg atgagtttca taactggctg
     1921 cacgacgctg caggctacac tatcatgcgt aatcctgcaa acgggttcta cgtttatgct
     1981 gttgaaaaga atgggaagct gacgtcatca gactatgttg taggaaaagt aaatcctcga
     2041 aactcaaagg atttaaagcc ctacgtgaat atttcgcgta atgagtacct ggctaaacgc
     2101 caccgcaagc aaaccgacaa ccatcatgta aaccttgata ttacaaaagg gactagcggt
     2161 attgccccaa ttagagcggt taagggtgtt accatgaaca acattgtggt ttttatccgt
     2221 tttaaagatg atgttgtagc cgatgagaat atttcctact acgaaaatgt ttacaacggt
     2281 tcgtcagcat cggtagccaa ctactactct actgtttcgt acggtaagtt cagtgttagc
     2341 tccacctttt acccccagct caacggcacc cttgtaacct ggtatgttga cgagaacaac
     2401 cggaactact accaacccta cgatgccacc acaaacccaa atggctacaa ccccgacgct
     2461 tcgaactaca acgatccaac gagttcaacc taccgggagc acaccctgct gaaaaaagcg
     2521 gtggaggctg ttggggcaag cattccaacc ggaattaacc ttgatataaa tggcgacaac
     2581 tacattgacg taatttcgtt cattttagcc ggtagcaatg actcgtggaa cgacctgctg
     2641 tggccccacc agtggagcct ctggtcgcag agtgcattca ttaacggtaa acaggttggc
     2701 gattacaccc ttcagctgca gtacttcgga ggttcacaaa ttgacctggg taccctctgc
     2761 cacgaaatgt tccatgcagt tggcgcaccc gacctatacc actacgacga tgatctaaac
     2821 cttagtcctg tcggctattg ggatatcatg gaaggaaccc tggactaccc ccagaacatg
     2881 ggtgcccaca tgaagtacct ttacgggggt tggattgacc aaatacccga aattactact
     2941 tccggcacct acacgctttc acctttggcc acatctccaa ccggcaacag ctacaaaatc
     3001 ccctcgccca acagctacag tgagtacttt gtagtggagt acaggaaaaa ggaatcctac
     3061 gacagtgatt taccgggcaa cggacttctg gtgtaccgaa taaactcgaa gcttgaagga
     3121 gttggaaatg ccgatgggcc tcccgatgaa gtttacatat accggccaaa cggaaccccc
     3181 actttaaacg gctccattaa cagcgcacac ttcaactcgt ccgtggggag aacaagcata
     3241 aacgacaaca cgaaccccag cagttttctc accgatggaa cgaacggtgg cctgaaaatt
     3301 tccaacatta ctgccgccgg tgaaaccatc tcgtttgatg caacaataga ctttatcccc
     3361 tacgtagtgc taaaaaatga caggggctat gccacagcaa ttggagatga aagcactaca
     3421 ctgaccgttg ccactcgctt taccactcaa gatttggcat cgctggtagg cagaaaaata
     3481 aagatggtgg atttctacat caggggagag gatggaaccg atgtaaccac caacgaaacg
     3541 gttaaggtat gggaaggggg aacctatggt aacccgggtt cccttatcta ctcaaaaaat
     3601 atatcctcag aggttgttcc cgaccaatgg acgtcgcaca ccctctcaca gcccatcgtg
     3661 atacagccca acaaggagta ctgggtggga tataccgcaa cagcgtcagg aggctacccc
     3721 tttgccaccg atggcggccc aatggtagca ggcaaaggag gatggattta cattgacaat
     3781 caatggtatg aacttatcaa cataaattcc cagctcaact acaactttct ggtaagggct
     3841 gtggtggggt ctgaaaccag cgatgcagaa accctgacag ccgatgcggt aaatttcaag
     3901 gtgtttccaa atcctgcaaa tgccaactca cgggtaagca taaccctccc aatggctggt
     3961 agggtagcag ttgacctcta caacatgctt gggcagcggg ttggcggtag catgcccacc
     4021 cactatgagg ccggggtgca gtccttactg ctaagtggta atgagctatc aaatggaatc
     4081 tacctgctta ccgtaaccta cgattccggc actcatcaca ctgttgaaac gcaaaagata
     4141 gtgatagcca gatagggtca attccttgat ctgatacaac caaaaaaagc aggagaaaca
     4201 cgctcctgct tttttttaat ttgaatatgc agctataata ttaccatcgt atgcagggca
     4261 atcttcaaaa aattctacct taaatttagg gtaagaacga agcttatacc atgaactttt
     4321 accaactagc tacagactgg agcaactcat caaattcagc cggagaacct tagccgaata
     4381 acaagcagct atagccctag tttctccctt agcttcctac catcagcctc gtagccgggc
     4441 ttacccagca gggcaaacat gttgcgttta taggcctcca ctccgggttg gtcgaagggg
     4501 ttaaccccaa gcgtgtagcc gctcagcgca catgcccttt caaaaaagta gaacaagtaa
     4561 cccacccagt agggtgaaag ttcgggaata ctgatagaaa tgcagggtac accgccatcg
     4621 ctatgggcag caatggttcc catttgggcc atcaggttga cctccgagaa gcgtttaccc
     4681 gagaggaagt ttagctgatc gaggttatcg ggatcgtctg ggacaacaag gcggtgggag
     4741 ggtttttcga ccgaaagaac agtttcgaaa agcatacgtt ctccttcctg gatgtactgc
     4801 cccatggagt gaaggtcggt ggtgaagtca acgccggcgg ggaaaattcc cttaccttcc
     4861 tttccctcgc tttcgccaaa aagctgcttc caccactcgg taaggtagtg aaggcgtggg
     4921 gtgaagttaa ccaaaatttc caccttcttg cccttttggt aaagggcatt gcgggctgca
     4981 gcatatatgc atgcggggtt ctggtcaaaa gctacagatg aagaggtgtc gtccatggct
     5041 tttctggccc cttccaccag cttcacaata tcaattccgg caatagcaat ggggagtaaa
     5101 ccaacgggtg ttaaaaccga aaaccttcca cccacatcat cgggaatgac aaaagtacgg
     5161 tatccctcgc tatcggccag cttacgcagc gctcctttcg acaggtcagt tatagccaca
     5221 atacgacttg cagcctcggt tttcccaacc tgtctctcaa gatgctgctt cagcacccta
     5281 aaggttatgg caggttcagt ggttgtgccc gatttggaga taacaacaat tgagtagcgc
     5341 ttagtttcga ggagttcgag cagttcggca tggtaatcct cacaaatgct gtttcctgca
     5401 aaaattattt taggcaaaac gtcctcggta agctgggtaa agctgctact tagtgaatca
     5461 atagcggcct ttgcccccag gtatgaaccc ccaatcccaa ctatcaccac tacatcactt
     5521 ttagcccgaa gctcactggc tgtatgttgt atatcgtaaa gttgttcctt agtaatactt
     5581 gagggtaagt tgagccaacc cagaaaatca ctacctttac cggtaccctg ctcaaggagc
     5641 agctgatttt gcgctatttt ggttttaagg gcaaagactt cctcctttgc tacctgggta
     5701 taaatgtttg ataagtcaac tttcaactga ttcattttta taaattaacg ggttaaaatt
     5761 tcatctacct tagctttgct gtcaacagct tcatctcaat agcaggcgag atattctcgt
     5821 aaagaatgtt gtaaacggcg ttggctatgg gcattttaac attatacttt ttattaatct
     5881 cccaaatgca cttagctgca ttatacccct cggccaccat gctcatctcc agcatggccg
     5941 atttaacgct gtagcccttc ccaatcatgg tgccgaaagt gcggttccgg ctaaactgcg
     6001 agtagcaggt aaccagcaaa tcgcccaggt agaccgaagt acttagcctt cgcttactgg
     6061 gatatgtttt cttaagaaat cgctcaattt cacgttgggc attgctgata agcacggcca
     6121 aaaagttgtc gccatagtta agcccgtggg caattcctga ggcaactgca taaatattct
     6181 taagcacagc gctatactct gtgccataaa tatccgttcc aatattagtt ttgatgtagt
     6241 gtgtttcaaa gtaggacgca acatgcttag cctgaagctt gtccttgcaa gaaattgtga
     6301 ggtatgaaag cctctccaga gctacctctt ctgcatgggt aggcccacta acaattccaa
     6361 ttcggtcaaa aggaacgcca tactgctggt taaaaaactc ggcaatggtg caatccattc
     6421 cgggaacaat acccttgatt gccgagataa taaacttacc ttctaaacta acactaaggg
     6481 gttccagcca ggcttgaaag taggtggatg gaataacgaa aagtaaaatg tcgtataatt
     6541 ttgcaacctc gttgatatcg acgcttaaat acaacttttt aacgtcaaac tcaaccgcac
     6601 taaggtagtt ggggttatgc ctgtactttt taatatagcc aattaactcc ggatccctaa
     6661 ggcaccagcc aattgagtcc tgtgtttcat gtagcacctt tgccagtgcg gttgcccagc
     6721 ttccgcttcc aactacggca atagaaggtt ttttctcaat ctcagtattt gtactcattt
     6781 cagatacttg taggtttgac ggttcgcctt aggcttacta catttgcact tatacttttt
     6841 ggtgtactta gcctttcccg atttttgaac aaccgttctg ttgctacacc cgaatagggg
     6901 gagcaatatc acaaaagcaa gaatgaaaag aatgctcttt tgatatgcag aatgtgctcc
     6961 tgtccgattg ctcatttgga ttgatgcttt cgtggataca aaggtaaaaa gaattgccaa
     7021 gtattcaatt ttacatggag gcccaataat cagaatagcg cttacagcat tggttgatat
     7081 gttgatatgc ctttcagtaa acctgtgcta attgaagttg atggaaaaac aaaataacaa
     7141 ggttccgctc ataggtttca ctccacagga gctttgcagc accatgcagg aatatggtgg
     7201 aactagcttt caggctgaaa gcctcatgcg ctggatatac cgtaaacgcg aaaaggatat
     7261 ttctaccttc tccgatatac caaaagctat tctgcaatca gttgaacagt cctatacggt
     7321 aggtctatac cctccaaaag aaagtaaacg gagcgacgat ggctctgtga agtacctgtt
     7381 tgccatcggg caaaaccagg ttttcgaagc tgtagccatg agttcaggca atcggcatac
     7441 cctatgcctg tcaacccaat cggggtgtag aatgggatgc agattttgtc gcacaggaag
     7501 catccgcttt aatggcaacc tatcagcagg cgacatcatc aaccaggtgc ttagcatacc
     7561 agatagcaaa aggttgaaca gaattgtgct gatgggtatg ggcgaaccgc ttgataattt
     7621 gaaagttgta aacaaaacgc ttgaaatcct gacagccgat tggggttgcg ccttaggtaa
     7681 atcgaatatt accatttcaa cagttggttt gctttccgaa ctggaggcct ttctaaaatc
     7741 gcccggctgt aacctggcaa ttagcctgca ttcacctttt cccgatgaaa gggcaatgct
     7801 tatgcccgta gaaagaacaa atccaatact tgaaataatc aagctgctca aacactaccc
     7861 cctaaaaaaa ccgctaaggc tcacctttga gtatgtcgcg ttaggcagcc taaatctatc
     7921 taaaaatcac gccagggcaa tagcaacatt gctaagtgga ctaagctgcc atctcaacat
     7981 tatcccatgg aaccggcaca atgcagcaac cttccaatca cccaccagcg acgaacttac
     8041 taactttatg gagctcctca acgatgcagg tatcccgaca aacctgcgca aatcaatggg
     8101 aggggatatt gatgcggcat gtggccaaat ggcgggcaat gccccccatt gagactaaag
     8161 ctggatcaat caaaatttaa taatcctaac agtaagcgct tatgagtggc taacccggaa
     8221 aattgtctcg ccgagaaatt tttcgatacg ggcaagctgg ggtctctcct ttttgttgac
     8281 gaaagtgatg gccacgccgt cgctttgggc acgcgcagtc cggccaatcc gatgaacgta
     8341 gtcttcggga tcggcaggaa cgttgtagtt aaccaccagg tctatgtcct caatatcaat
     8401 tccacgcgca acaatatcgg tagccaccag cacctgaaat tttcggttcc ggaaatcccg
     8461 aagcacctgt tcacgctggc tttgctcaag gtcggaatga attgccctag ccattatgct
     8521 ttcccttacc agcttgcgct caatgtcctt tacttccatt tttttagaag caaaaaccag
     8581 aacgctttgc acgtactcct taccggccac caacgactgt agcaatccga ttttttctga
     8641 atcctccacc atgtaaaccg cctgcaaaat actttcgaca ggtttagaga ccgaaaggct
     8701 tatttcaacc ggattgtcaa ggatagtctt ggccagctgg cggattttag gaggcatggt
     8761 tgcagagaac atcaaggttt gccttcgggt tggcagctgg gagataatcg ccgtgatatc
     8821 gtcaaaaaaa cccatgtcga gcatgcggtc ggcctcgtca agaattaaat gctccagcct
     8881 atccagctta acgtagccca tttgaatatg ctggaggagc cttccgggag ttgcaactat
     8941 aatatcagcc ccctgctcca gggccatgcg ctgccgatcc cagctgttgc catcattacc
     9001 cccgtaaacg gcaatggagc tggttgaaat gaagtaggca aaaccctcta cctgctggtc
     9061 aatctgcagg gcaagttcac gcgtaggaac aattatcagg gtgtttatgt agccttcagt
     9121 ggtaggcttt tcggctattt tacccagaat aggcagcaaa aaagcagctg ttttcccagt
     9181 tccggtttgt gcacaggcta ttaaatcacg cccctgaaga attaccggaa ttgcctgctc
     9241 ctgtattggg gtaggatttc cataccccat tgtcgaaatc ccctcaagta tgtgaggatt
     9301 aaggtttaac tcgctaaaat tcaagtttaa tgtttgttga tgtattcgga tgcaaagata
     9361 ctgttttcaa atttaggtga aaaagtgctg ctaccctgcc tggtgctggg ttaaatattc
     9421 aacctaaaag acgcgcagaa gcgctatttc atgctatcat aaattttcac cccccccttt
     9481 tttggtcaga atattcatta aatactctct gtaaaccgct accattctga attatttcat
     9541 ttataaatat tgccctactc cgttttctta tgcctttagt tctggctaat tgtggggaag
     9601 taacctggta gctataattt tgtaaattac tacttttgca gcgaaaagga gcgggttatg
     9661 aggattaaat ttttcagcat tagcaatagg tacaggtacg tttttattga tttggataga
     9721 actctttggg attttgagcg aaacagcctc gaagctctac atgagctgtt ttcggaatac
     9781 aagcttgact cggtagttgc cgatgccaca acattttccc gcacgtatgt aaagcacaac
     9841 aactacctat ggaatagcta ctgcaaaggg gagctaacca aggaggtgct gcgatacaaa
     9901 cgatttgatg ccacactttc cgagttagga gtaaacaatc accaacttgc agccaaactt
     9961 ggcgaggagt atctccgcat attaccctta aaaaccatcc taattccaaa tgccaaggag
    10021 ctactcgatt accttgcgcc aaagtacggg cttatgattc tgagcaatgg ctttcatgaa
    10081 gtgcaggttg ataagctgaa aaggtgtggg ctgaatcact acttcgacag ggtagtaacc
    10141 tcagaacttt cgggctacca taaacccgac ccacgcgcct tcggttatgc actttccagt
    10201 gcaaatatca gaaaaaatca gggcattatg gtgggagacg atttagaggt agatatagtt
    10261 ggggccagga agtttgggat ggaccaaatc ttcttcaacc cagaaaggaa accgcacggg
    10321 gaaaaagtaa cctacgaggt ggaaaccctt aaggaaatca cccaaatact ctaaagcggg
    10381 aaaaagcagc aaattttctt ttgcagaaaa aatgagttgc aaatctgggg catatcaatt
    10441 aatcgtcaaa aaaactctac acctacatcc tctcttgata cgctactagt tcaactaaaa
    10501 aggaatatcg ctgctatcaa ccccttgccc ggagagaaaa tcataatccc cgtgggtatc
    10561 ctcgttcatt ttagagctga aggttacggg caccgacgag gtgaaaggtg aatgcgaggg
    10621 ctgaatatcg ccatagtgca cgtcgtcgaa atcggaaaat ttcgccatct ccttacggaa
    10681 gttaagctta atatcatcaa cagggccatt acgatgcttg gccacaataa tttcggcaat
    10741 cccctgggtg gaatttccat cctcatcctc cataaaacca tagtactccg gccggtgaat
    10801 gaaaatcacc acgtcggcat cctgctcaat agcacctgac tccctaagat cggagagctg
    10861 cggtcgttta tatccccctc gtgtttcaac cgagcggttg agctgcgaaa gggcaataat
    10921 gggaacgttt agctccttgg caattgcctt caatgagcgg gaaatggtgc ttacctcctg
    10981 ctcacgattg cccttggttt ctgaagggcc ggtcataagc tgcaggtagt caataattat
    11041 cacgcccaca tcatactgtg ccttgagtcg tcggcacttt gccctaaact cgaaaaggga
    11101 aagtgcgggt gtatcgtcaa taaagatttt ggcttccgaa agatttttaa ttttaacggt
    11161 aagctgagtc cactcgcttt ggtcgatccg gccattgcgg attttttcgg acgcaattcc
    11221 cgactcgcta acaataagcc tgttcaccag ctgcaggttc gacatctcga gcgagaagaa
    11281 cgcaaccgga acgtcgtggt caaccgccat gttcctggcc atggaaagaa cgaaggcagt
    11341 tttacccatg gatggacggg cggctacgat aatgaggtcg gaattctgcc atcccgatgt
    11401 aagcctgtcg agcgctgtga agcccgaagg cacaccgctt aacccgtctt ccctcttccc
    11461 cgcttcctca atctgcttaa ttgccttttt tatgatggtg gtaatgggtt gcgtatcgct
    11521 tttaatgttt ccctctgcaa ctttaaacag ctccatttcg gagtaatcga gcagctcatc
    11581 aacgtccaca tcttcatcga acgacctgtt ttgaatatcg gttgaaaccc gtatcagctc
    11641 acgctgaata aacttttggg caataatctt agcatggtat tccagatggg ctgctgaccc
    11701 caccttggtg gtaagttgtg ccagaaaagc ggctcctcca atctcctcca acttgttctg
    11761 cctcctaagc tcctcggtaa ccgtaaacag gtcgatgggc tccagcctgg atgataaatc
    11821 ctgtatggcc ttaaaaattt cctggtgggt ctccttatag aagcattcgg gtttaagaat
    11881 ttcgctgact atgacaacag catccttttc caccataata gcgcccagca ctgcttcttc
    11941 aagttcaatt gcctgcgggg gcaccttgcc catttctgcc gcgaggggtt tagccaccct
    12001 ggtcttcctg ctgcctgtag ggttcttttc tgccatatca tttttcttag cccacaaagg
    12061 taagaatagc ccagctctta accccaatgg gtggcaaagt gttttttaac aaaagagaca
    12121 ggcttacaaa caagttatta accacactaa aatattggtt gatgcttagc taattttaat
    12181 actcttgttc cataacgctt tgctatcttt gggggcaaat gtcaacctgc atgaaccggg
    12241 tcatactctt ttcacttact gctcttgtgc tcctcatttc ctgcaaaccc aacaggactg
    12301 agaggattga cccgaccaat gttccagcta gcattcagct ggagaggttt gagcaggatt
    12361 tattttctgc ttcgcccgac agcctgcaga ccctagtacc cgagttaaga aagaagtacg
    12421 ggcgttttgt cgatttattc tccgagggaa ttatccatat tggaaaaacc gacagccccg
    12481 agtactgcaa ttacctgtcg caatttctaa gcgataccat ggtgcaaaac gcctaccgat
    12541 tatcgcagca aaagttccaa aacataagca gtattaaggt ctgccttaat gaagcattca
    12601 atagatttca ctgctacttc cccgataagc cagttccaaa aatttacacg tttgtctcgg
    12661 ggtacaacct ttcgttggcc gttgacgaca gcattctggc tattgggctt gaccgatatc
    12721 tgggcagcaa taccgttcag tataagctgc tgggtattcc tcgctaccag gtgagaaaca
    12781 tgcgtccaga aaagatacct tcggatgcca ttcgcgcctg gatgtatggt tcgttcagct
    12841 tccacgactc tactgacaac ctgctaagca acctaatata tgagggacag gtgatgtacc
    12901 ttaccaagca aatgctaccg caggagtccg attcgctgat ttttggtttc acacccagcc
    12961 agcaccgctg gtgcctgaaa aacgagaaag cgatgtggac taatttaatt gagaataagc
    13021 ttctttttag caccaacacg ctggatatta acaagtttat taatgatgca ccctttacct
    13081 cgggatttcc acaggagtcg ccaggcaggg ctgccgtttg gataggctac agaattgtgg
    13141 aatcgttgat gaaaaataac gataccctta ccctaagcga aatgatgggc atatccgact
    13201 accaaaagtt tctggcaatg tcgaggtata aacctagata gtaagctact tgcaaatttc
    13261 acttttaaag attttcaaaa cgaagtagaa atagcattag catgaacccc aattgcattt
    13321 ttaaaaagct gaaggcacca acaaaatgga ccaaaaaacc atcaaagcat aaacaaaaaa
    13381 tgaaacagct tgttgctaat cattaatatc agtaagtaat agcgcaacaa catcatttaa
    13441 ttttcaactt acctgtcttt taaattttca tattcaaccc tttggaacct acaaagtata
    13501 aatacaaact attttaaata tctattataa tgaaatcaga cattgaaatt gcccatgagg
    13561 ttaaaatgaa gccagttacc gaaatagcgg gcaaactggg aattgatgcg gacgacctgg
    13621 tgctttacgg gaagtacaag gctaaggtgc cgctaaagta tatcgacaaa aacaagtacc
    13681 agaaaaacaa gctggtgctg gtttcggcca tttcacccac cccagcaggt gaagggaaaa
    13741 caacggtttc aataggcctg gcgcaagcgc ttaaccggtt gggaaagcag actacggtgg
    13801 ttcttcgtga gccctcatta ggcccggtat ttggagtaaa gggtggtgcc accggtggcg
    13861 gatactccca ggtgcttcct atggaggaca tcaacctgca cttcaccggc gattttgccg
    13921 ccatcgaacg tgcccataac ctgttggcag ccctgattga caacaacatc caaagcaaaa
    13981 aacgctcact cggtatagac ccccgaaccg taaggtggaa aaggattatg gacatgaacg
    14041 accgctcgct ccgacacatc atcgttgggc tgggaggaac atcacatggc gtccctcgcg
    14101 aaacgggatt tgacattacc gcagcatcgg aggttatggc cattctttgc ctggccgaga
    14161 atatcgacga cctgaaaaaa aggctgggca acatctttat tggtttcaca tatgataaaa
    14221 agcccatcta cgcccgcgac ctaaacgccc agggagcaat ggctgcccta ctgcgcgatg
    14281 ccatcatgcc aaacctggtt caaactattg agggtacccc ggccattatc catggaggcc
    14341 cattcgccaa catagcccag ggaaccaact cggttctagc caccaagctg ggcctgtcgc
    14401 tcagcgactt tgtagttacc gaggctggat ttgggtttga ccttggcgcc gaaaagttta
    14461 tcgatatcaa gtgcgagtat gccggcttaa accccaacac cgtggttctg gttgcaactg
    14521 taagagcgtt aaagtaccat ggcaataaac cgttgaagga agtatcgcag cccgatttgg
    14581 aggcgctcaa aagaggaatt ggcaacctgc agaaacatgt agaaaacatg aagcaattcg
    14641 gtatatgccc ggtggtagcc atcaaccgct ttacctccga taccgatgag gaggttgagt
    14701 atatcaaaca ggtgtgccgt gatatgtccg ttcaagtagc cgctgttgat gtttggggaa
    14761 aaggtggaga aggagctatt gagctggccg aaattgtgaa caaaattgca tcgagctgct
    14821 ctgctcagca caaaactctt tacgattgga gctggacgcc cgagttaaaa attgaaaccc
    14881 tggccaagag gatatacggc gctgaggcgg ttgactacac cccccaggca aaagctgacc
    14941 tggaaaaggt ttactccctt ggcctcgata agctgcccat atgcgtagcc aagacccaaa
    15001 aatcgctatc ggacaacccc gacctgttgg gtcgtccaaa ggattttgta gtaaccgtgc
    15061 gggaaattga agtggcctcc ggagcgggat ttattatccc cataaccggc cagattatgc
    15121 gtatgcccgg gttgcccgat gaacccgcag ccgaacgaat ggatgtggat aacaacggag
    15181 taattactgg gttgttctaa ttatctaaat actaaatgaa caagagttaa ggagacgggt
    15241 tcaaataacc tgcctccttt tctttttaaa aggccagctc caccataaat tcgattacct
    15301 cctcaacctt gccactactt tcattacggc tgtaatcaac aacaagttta ttgcgtctaa
    15361 ataaactata ggcaactcct accacgctct gcgtaatgtt tccaaattca gagagatttc
    15421 gacccttaac ataatcgtaa cggccaaaga tggagagcct attccgtaac aacttaaact
    15481 caacaaaagc actccatccc cataccttat aggaaacacc aagggtgtcg gctaaagaac
    15541 cgctataatt accaacactg ctaaacacct gcccacaaag tgtaacgggc tggccctggt
    15601 acgacagcac acccacgttg gtattccaat cgggagaaag cgtggtgttt ccctttccgg
    15661 ttgcccctgt ataggatagc tgtaaaccgg gaatcatgtc tggtaaggga cgtagggtta
    15721 accggtactg taaggtcttg ttattgtttt cctctatgtt atggtaaccg ccgccattat
    15781 acacaccaat ggccaacgat ccatacctgc cgggatagtc actaccaacc cgtgtctggt
    15841 aaccttcatt aagtgttcct cccagcaatg cagctgccat caccccaaaa tcggctgaaa
    15901 tcaaaatatc tgacttctct agaaaatggt taccctgaat cctatagggg ttgattttct
    15961 cctcaaactc aatccacgga gtgaacacct ctcctataaa tacagcagga ttggtaaaaa
    16021 tccagaaatc acccggctct tccagcttca tatagcagta cttgagccgc atttccatat
    16081 caccctgacc atctccctcg cgatcgagag taatatcagg ggttattctt cccgaaatat
    16141 tcttgttaag cgattttatg aagttgatat agcctcgctt tacagtaaat tcattagtgc
    16201 tcccactatt tccgataaag tagcggtaac ctaagaaaat ttgagtagaa actgaaatat
    16261 cattcggctt gttagccgac aattcctgat tatttaatct aagagtgtca gcaacctgtt
    16321 gcccaaaaac tttgggaaaa gtgaggagaa gtagaatcgt aagaataaac caacccttca
    16381 tcattttaac atttttagca aaaatatgta aacatcccaa ctggtgcaac catgttagga
    16441 gttgctgccc cactaaaaaa tatgtagatt tgcaaagaaa tctaaaaatc caggtagcat
    16501 gaaatttttc attgacacgg caaatcttga ccaaattcga gaagctaacg atttgggcgt
    16561 tcttgatgga gttaccacca acccttcgct gatggccaag gagaacatca ggggtagtga
    16621 gaatattaaa aagcactatg ttgatatctg ccacatagta aagggtgatg ttagcgccga
    16681 ggtaattgcc accgattacg aaggaatgat aagagaaggc aaggagctag ctgcactcca
    16741 ccctcagatt gttgtgaagg ttccgtgcat acgggagggc attaaggcga taaagcattt
    16801 ctccgatagc ggtatccgta caaactgcac actggttttc tcggcaggac aggcgctact
    16861 tgctgcaaag gctggggcaa cctatgtttc tcccttcatt ggtaggttag acgacacttc
    16921 caccgatggt gttgcgctta tccggcaaat tgttgaaatc tacaggcact acggttacca
    16981 aaccaaggtg ctggctgcct ccattcgtca taccatgcac attatccagt gtttagaggc
    17041 aggagccgat gttgcaacat gcccattgaa tgcaattctt gggctactca accatcccct
    17101 tactgacatc ggacttcaga agttcctcga cgactttaaa aaggttaacg gttaatgccc
    17161 gagtgcctct acattaaaat gtaccctcag aacctcaacc cccgcgatat tgagcgtgcg
    17221 gttgaggtgc tgaagcgcgg cggagtcatc atttatccca ccgatacggt ttacggcatt
    17281 gggtgcgaca tcaccaacac caaggcggtg gagcgcatca tcaagataaa gggaatgaag
    17341 cctaaggaag ccaatttttc atttatctgc cacgacctta gccacatagc cgattttgcc
    17401 cgggtggata acagcacatt caggctgatg aaaaagaatt tgcccggccc atttaccttt
    17461 atcctccccg gtcttaaccg ggttcccgat tacttcctaa gccgccgtaa aacagtgggt
    17521 atccgcattc ccaaccatcc catacccgtt gaaattgtcc gtcagctggg aaaccccatc
    17581 ctcaccacat cgctaaagga cagcgacgag gtgattgagt acaccaccga tcccgagcta
    17641 atttttgaaa actaccagaa tttggttgac ctggttattg acggaggcgc aggtaataat
    17701 attccttcaa cggttgttga ctgtacaggc gacgaaccag ctataatccg tgaagggttg
    17761 ggcgaacttg tcttttaacc ctaccatgca acattccatc acgctggcta ttcttgccag
    17821 tggcaaagct accagaatgg gtagagttgg taaggctttt ttaaaagttg aaagaaaaac
    17881 caataattca aagaatttac gaaagccttt cgagcttatt tacccaagta atcaaaatca
    17941 caaacaccac aagtacagaa tatcggactc cgtcaaatat ttaccccgac acgttgaata
    18001 aaggctgtcc tctaggcggt ttacattctg ccctgttcca ctccttcacg gaacttgtat
    18061 ttatagctgc atcggatacg ccctgccccg atagaaattt tgcctaaaaa atcatacagg
    18121 gtcatttcag catcaagcca atgacggaga tatttgcacc tagtataaac gggctttccg
    18181 aaccttgatt tggaatttac tctaaaacgg agcctttcca tggttgaaga aaccttatgg
    18241 gatggttttg atcatatcat gtcagaattg catcgggctt gtgtaaccaa tcacatggat
    18301 ttgcccccta ccccttctgc taaaggcgct tgttacaaca tcaacttgcc tgccgacctg
    18361 aataatttac cagccaaaga aagcctgggt agcgtgttgt ctagacaaaa gcaagctaaa
    18421 atcaaagaaa gcttgtcggg ctctgagtga aactgtcggg tttctgacgg aacaagtccg
    18481 accttcgaca gattcgtttc tccagggact ccgggcagat tttggatata aaaaagaaaa
    18541 ccctttgcag atttcaggtg caaagggtta agtattctga tcgggcaacc tttcacattt
    18601 cagcgacttg tctagtcctc ccggggctag catcaagtat tcgcttcaac gtatggttgc
    18661 taaaagttaa aactgcgcaa ttttttcctt gttatactta ccggcagcta acctgtcgcg
    18721 taccaccctg aatgcctcaa cagtttcctt tacatcgtta agggtgtggg ctgctgtagg
    18781 tatcaacctt agcataattt ggcctttggg aaccacaggg taaactacca tggagcagaa
    18841 aacgccgaag ttttcacgaa ggtcgaaaat catattggcg gcctcaggta tt
//
LOCUS       DAOPHX010000038        18782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_3225_length_18782_cov_6.476424, whole genome shotgun sequence.
ACCESSION   DAOPHX010000038 DAOPHX010000000
VERSION     DAOPHX010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 18782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18782
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3225_length_18782_cov_6.476424"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="PK502_04395"
     CDS             complement(<1..1262)
                     /locus_tag="PK502_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPH00089.1"
                     /translation="MKRLLYIFVLLVLADGVSNAQQLLTVEQCRQMALEHNQRMLIAG
                     EHVQAATSLKSSAKTQFFPSVSANGLYMRTNKKFSLFENDLFLPVIPYSAIDPSTGSF
                     NPNLDPANTFVINPSTLQPVTDANGNPIFQNYTWLPKDQAEVGYKNVYTAGVSLTQPI
                     FTGGKIRETYRIAKYGESLARANEAAERSDIIYKTEEAYWRVVAVGEKVKLVNASIQL
                     LEKLSVDIENFYEEGMVIQNDLLKVKVKLNEAQLNLVKVQNGLALSKMALCQQVGLPL
                     NQEILLADSLVASMDTAVIMQSFTDTALAKRPEIEMLNQTINIAKSGVNLMRSRFMPN
                     IGLSANYMLFNPNPYNGFSENFGGDWNVGVVLNIPIFHWNDRGHTLKAAHSEQRVAEL
                     KMDEAKELISLQVQQAVYQLNESVKKVQ"
     gene            complement(1465..2067)
                     /locus_tag="PK502_04400"
     CDS             complement(1465..2067)
                     /locus_tag="PK502_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPH00090.1"
                     /translation="MHQEVIGSPHDEMRNSIINAATKAFSRFGFRRTSMDDIARSLRM
                     GKSSIYYYFKGKEEIFRAVVAREASQQRENVKKILESPDDAVSKMRSYVKLRMEFIRH
                     LSSFVEQRLNDDLKSLQLTEKFRKKYDDEEISIITQILEEGSRSGAFKVKDFYMSALA
                     IVTAMKGLELPLVNSTNDENAMNPVIDDLLDILFFGIVKR"
     gene            2452..3600
                     /locus_tag="PK502_04405"
     CDS             2452..3600
                     /locus_tag="PK502_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HPH00091.1"
                     /translation="MLFSDIVGQEKVKERLIQLVRENRVPHANLFVGPPGAGKLPLAV
                     AFAQYIMCENRGKSDSCGVCPSCRKVAKLVHPDLHFVFPTKTKEKSDEGADENSEKPD
                     DTISTWRSAIIANPYFGENDWYEMIGMENKQGIISTATASEIIRKLGLKSFENEFKVI
                     IIWLPERMNNQAANKLLKLIEEPPEKTIFLLVSENPTHILKTILSRTQQVMVPPINRE
                     SVALALHDKYNLSPELAHDISRIANGNFYEAIRLKDQESNYFFTSYRKLMRLCYSKNV
                     LGLLDWVDETASLGREDLKHLLVYSLRLTRETLVLNMGVEEVSYLMGEEEAFCHDFSP
                     FITTNNVFSITDELVTAFECITQNGNPTVVLTHMTMRMVKLIPNKKAA"
     gene            3654..4814
                     /gene="ricT"
                     /locus_tag="PK502_04410"
     CDS             3654..4814
                     /gene="ricT"
                     /locus_tag="PK502_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPH00092.1"
                     /translation="MEKNIGSRGGVITVNEASEHSVCSSKGCAKLKVHDWMADLPMPL
                     QQSDIIEVQFKNTRKGFYRNANQLVLRQGDIVAVEATPGHDIGVVTLTGWLVERQMKR
                     VGVDPKTTEFKKVYRKAKQADIEKWIEAIALENSTMIRARKIAEEQRLNMKIGDVEYQ
                     GDKTKAIFYYIADERVDFRELIKILAEEFRVKIEMRQIGARQEAGRIGGIGPCGRELC
                     CSSWISHFASVSTNSAKVQELSLNPQKLAGQCGKLKCCLNYELDSYMDARKDFPDTSI
                     ALETADATYYHFKTDIFRRSMWYGSEPSSSVNLIEVSVDRVNEIIALNRKGIKADKLL
                     ENTTSDQNNSRPNGFTDGLLDEGSLTRFDKKGGKTAKRKKSRNRNRTRPSNE"
     gene            4807..5286
                     /locus_tag="PK502_04415"
     CDS             4807..5286
                     /locus_tag="PK502_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HPH00093.1"
                     /translation="MSKSLPIAFLAATLTLACCNRDAVFDSYVAIPRETWSADSLASF
                     NIAIPDTSQSYDIIINLRNSTDYPFQNLFLFISIYSPNGGFVRDTFECFLADDNGRWT
                     GRGSGRIHDNRFIYREGVKFYQPGSYRFDLQQAMRVEDLRGVVDVGIRIEQSQQNFK"
     gene            5341..6039
                     /gene="trmB"
                     /locus_tag="PK502_04420"
     CDS             5341..6039
                     /gene="trmB"
                     /locus_tag="PK502_04420"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPH00094.1"
                     /translation="MGKNKLKRFAENETFSNLFQPPLEEVLNDKFELKGKWGNSYFKN
                     INPLVLELGCGKGEYTVSLAGMNPQKNFIGIDIKGARIWRGAKTALENKLNNVAFVRT
                     RIEHICSIFAQNEVDELWITFPDPQEREIKAKKRLTSSRFLNLYSGFLKPNAIVHLKT
                     DNRQLFDYTKALVEQNKFNVLICTTDLYANFTNDPVLGIKTFYEQQFLGEGLPITYLR
                     FVLNHPGPYHEPIR"
     gene            6023..6367
                     /locus_tag="PK502_04425"
     CDS             6023..6367
                     /locus_tag="PK502_04425"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPH00095.1"
                     /translation="MNQSGNDNTGFFEKVYEVTRMIPYGRITTYGAIARYLGSAQSAR
                     MVGWALNSCHSQPDWVPAHRVVNRNGQLTGKVHFPGSNTMQQLLESEGIRVEDDTVLE
                     FKKFFWNPATEL"
     gene            6483..7586
                     /locus_tag="PK502_04430"
     CDS             6483..7586
                     /locus_tag="PK502_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPH00096.1"
                     /translation="MNLTTDQVLEVLNQVVHPESGQSIVSMGMVKDLTASDRRISFTL
                     KLTKKNDPFASSLKKTAQRLLTERVGQQVEIDIQVVADIPERISPLKTKASVSRVKNI
                     VAIASGKGGVGKSTIAANLAVSVAKSGARVGLIDADIYGPSIPKMFNVENVQPEVMKE
                     DEMELIVPVERYGVKLLSIGFFVDPNNALIWRGPMATSALKQLIHQGAWGELDYLFID
                     MPPGTSDIHLTLVQEVAVTGAVIVSTPQDVALADAVKGISMFGSQGINVPVLGLVENM
                     TWFTPEELPENRYYIFGKDGCKKLAERMKLPLLGQIPLVQSIREGGDFGEPVALHSSV
                     VAKAFDELATNLIRQVEIRNRELPPTTIVNVKH"
     gene            7616..7870
                     /locus_tag="PK502_04435"
     CDS             7616..7870
                     /locus_tag="PK502_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPH00097.1"
                     /translation="MTSKEEIVTKVAAAIDVIRPFLQNDGGDISLVEVTDDNTVRVKL
                     HGACGSCPYSLMTLKNGVEQAIMRDVPEIRGVEAVNLNIP"
     gene            8100..9227
                     /locus_tag="PK502_04440"
     CDS             8100..9227
                     /locus_tag="PK502_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00098.1"
                     /translation="MRYVLLFTLMLVVASVAAQEPVTLKFTRPSKVQASASKIKLKVQ
                     GNEYVLKNGASISINVTPEYGKSLLINAGDQTAYYLKPKPGETYEFEVGFKFAGPYIL
                     LLSGEEAKPGEAVQVTDTTAKPEGWETNLKVDRDNMGAGITLQKTDQSEAIRQEWLAR
                     GGKIKYQSVVFNGTYFGMDMDMEGVDANRIDGYGGGISFYQNRINLKIPAYKTGASSW
                     NSFNIGFGYDMLVYAYKFDMKMDPITTKMDVVTITMPFSANIGWTLGLGKFIDEGNWK
                     GVALTLRYRPSIMFNFTNSTVKMTSTDPHISGSTSTSTDSNVQFNAGGVGFDVDFSNF
                     SSTMEKLAPKPKLKLSFFVMPPVKDMPLFISFGLGITTYSR"
     gene            9442..9903
                     /locus_tag="PK502_04445"
     CDS             9442..9903
                     /locus_tag="PK502_04445"
                     /inference="COORDINATES: protein motif:HMM:NF015678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HPH00099.1"
                     /translation="MRTLGNILWHFPFLGFLTALGTFLIGGLLVITVIGSPLGLGLIQ
                     LSKFLLAPFSNAMVDKTDLQADQNKAWKTYGIIVRILYFPIGLFLVVVNIFQIAGLFI
                     SIVGIPVALVLAKSIGTYFNPVNKVCVSKAVADELSSRKAKAEVDKAFATK"
     gene            10105..10674
                     /locus_tag="PK502_04450"
     CDS             10105..10674
                     /locus_tag="PK502_04450"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HPH00100.1"
                     /translation="MLLLATLMCPALYAIPDTSRVALLVIDIQDFYFPGGKSELVNPL
                     PAAKNAALLVEYFRSHQLPVIYVKHQSAEQEEINEMVKPLADEPIFTKTEVNCFVGTQ
                     LYDYLVGQQISKLVICGMQTHLCVEAAVRAASDYGFKVTLIHDACATRDVKWGNTTIP
                     ANMVHLSTLSTLQNYARILSTEECLKANP"
     gene            complement(10687..11850)
                     /locus_tag="PK502_04455"
     CDS             complement(10687..11850)
                     /locus_tag="PK502_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPH00101.1"
                     /translation="MAKILVIDDEKAIRSTLKEILEFESHSVDLAEDGPTGIEMFGSG
                     CYDIVLCDIKMPQMDGIEVLEKLQEQAADVPVVMISGHGNIDTAVESIKKGAYDFIEK
                     PLDLNRLLITIRNATDKTTLIQEAKILKRKVNKAYDIVGESPAIVKVKEMIDRVAPTE
                     ARVLITGPNGTGKELVARWLHEKSNRSDMPFVEVNCAAIPSELIESELFGHEKGAFTS
                     AIKQRKGKFEQADGGTLFLDEIGDMSLSAQSKVLRALQENKISRVGSDKDIPVNVRVI
                     AATNKNLTHEIQNGNFREDLYHRLSVIIIHVPPLSERVEDIPLLAEHFNQLICSEYGI
                     PPKVFRPDAVDELKRLPWTGNVRELRNVVERLIILCDKDITGGDVKAFASPAF"
     gene            complement(11897..12826)
                     /locus_tag="PK502_04460"
     CDS             complement(11897..12826)
                     /locus_tag="PK502_04460"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00102.1"
                     /translation="MNVAIIDMGTNTFNLLVARFNQEERPEIIYEEKLPVRLGEGGLN
                     RKIITPEAWQRGLDAINTHMAKAKSLGAGKFFAFATSATRDASNGTNFVEDIRTSFGL
                     NVEIIDGNREATLVYKGVREALDLGNTCSLILDIGGGSNEIILGNTSEPFWLHSFNLG
                     VSRLMQQFQLSDPITQKQLRALEEFINVEVQTLFDAARRYVPVRLVGSSGSFDSIRSI
                     MEHSGSIAPGSEPWAEIPLERYFELHNFFITSTHDQRMALKGLDPMRVDLIVPASVFI
                     NLIIQKIGINKLFQSSFALKEGFMAELQSSHAF"
     gene            complement(12838..13479)
                     /locus_tag="PK502_04465"
     CDS             complement(12838..13479)
                     /locus_tag="PK502_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPH00103.1"
                     /translation="MDNDLQQVYQSVKFACRGDSTGHDWWHILRVVKLALFISERENA
                     NPFTVELAALLHDVDDWKLNRNTEGCPVALRILHESGYSDDEARRVALIIGQVSFKGA
                     GVDTTPSTIEAKVVQDADRLDAIGAIGVARAFAYGGARQRPMYIPDLKPQQHHSFDEY
                     QHAHGSTINHFYEKLLLLKDRLNTQTARQIAVERHQFMELFLTQFFKEWSVLD"
     gene            complement(13578..14930)
                     /locus_tag="PK502_04470"
     CDS             complement(13578..14930)
                     /locus_tag="PK502_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPH00104.1"
                     /translation="MKQMFLILQREFMTRVRKKSFIFTTLLSPLFFAAMVILPTYFAT
                     MEDTEVRNIAVIDNTQHFGSAIPQTEYIKITTITDTPAEKVKNSLEESGYYALLTIDS
                     LPGSIAPALTIYSPKQPSIEVISHIENSMEKEIETRKLMTYNIQDLDKILKDVKTKVN
                     VRSIKISKDGEEKESNTLITMGIAYILSFLMYMMVMLMGNQVMQGVIEEKNNRVVELL
                     VSSVKPVQLMMGKIFGIAAVGLLQIAIWIVLTAGLAGVGLNLISEKSGKANMEQVQIA
                     NQSIMANQGAQSADVTAAISETNTDNFLQALKNQNYGLIIGGFIFFFLMGYLLYAAMY
                     AAVGSAVENVSDTQQLVMPITIPLILAIFVMIVGIKSPDGSLAFWFSMIPFTSPIVML
                     TRLPFGVPVWQLILSAALLIGTFVVTVWLAAKIYRVGILMYGKKYSWKEMIKWIRYRS
                     "
     gene            complement(14955..15896)
                     /locus_tag="PK502_04475"
     CDS             complement(14955..15896)
                     /locus_tag="PK502_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPH00105.1"
                     /translation="MNLLETHGVEKRFSNHLALDDISIAIPKGSIYGLLGPNGAGKTT
                     LIRIINQITGPDKGEVMFDGRKIEPNDIYRIGYLPEERGLYKKMKVGEQALFFSQLKG
                     LSRQDATKSLKYWFEKFEIQSWWDKKIEELSKGMAQKVQFIVTVIHKPELLIFDEPFS
                     GFDPINTQLITDEILELKKNGTTIIFSTHNMESVEQLCDHITLINKSKNILSGQIDQV
                     RANYGSNVFEVAFKGNLETLRQNLNGTFDIFNVNTEKAIPRVQVRLQSASDSNKLLQS
                     LISSVEVLSFNPVVPSMHDIFIRVVQEYNKAHGLSLN"
     gene            complement(16057..16812)
                     /gene="rnc"
                     /locus_tag="PK502_04480"
     CDS             complement(16057..16812)
                     /gene="rnc"
                     /locus_tag="PK502_04480"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPH00106.1"
                     /translation="MRKFLPKAFWRLRVPAADHEIYRSIEQVLGFSPQNLELYKLALV
                     HRSASLVIAHGKKVDNERLEYLGDAILDAIVADYLFTRFPSQNEGFLTQTRAKIVSRA
                     SLNQLAIGIGLPNIVVLGNGASFNQKNIFGNALEAIIGAMFLDMGFKETSRLVVSNIL
                     ERQVDLEAIQHVEIDFKSRLLELAQKQKNSAEFLTKIESLPESAIPHFTSVLRWDGKE
                     IAHGHGTSKKEAEQNAAMQAMETITEGADKPTV"
     gene            complement(16822..18069)
                     /gene="fabF"
                     /locus_tag="PK502_04485"
     CDS             complement(16822..18069)
                     /gene="fabF"
                     /locus_tag="PK502_04485"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPH00107.1"
                     /translation="MEMKRVVVTGLGTINPIGNSVPEYWANLEKGVSGSELITSFDAS
                     KFKTKFACQVKNFDPNAYFDRKELKKIDPYTQFALVAVEEAMKDSGLNTEAIDLDMAG
                     VVWASGIGGMQTFTEELRGYFAGDGTPRFSPYFIPRMISDIAAGHISIKYGFRGPNFS
                     TVSACASSTNGIISALDIIRTGKASVIIAGGSEASVNEVGVGGFNSMQAISVNNDEYK
                     TASRPFDATRDGFVIGEGGGCLILEEYEHAKARGAKIYCELKGGGMSADAYHLTAPHP
                     EGLGARKVMENALKDAGLTIKDIDYINVHGTATPLGDIAEAKAIISVFGDYAFKLNIS
                     STKSMTGHLLGAAGAIEAIAAIFAICTDTIPPTINFKSPDPEIDSRLNITPNSAQKRS
                     VKAALSNTFGFGGHNASIIFQKI"
     gene            complement(18119..18355)
                     /locus_tag="PK502_04490"
     CDS             complement(18119..18355)
                     /locus_tag="PK502_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPH00108.1"
                     /translation="MSDIATRVKSIIVDKLGVDENEVTPEASFTNDLGADSLDTVELI
                     MEFEKEFNLSIPDDEAEKIGTVGDAISYIEGHSK"
     gene            18535..>18782
                     /locus_tag="PK502_04495"
     CDS             18535..>18782
                     /locus_tag="PK502_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPH00109.1"
                     /translation="MNNIAILASGSGTNTENIIRYFSNHPLIRVTLVATDNPNAFVIE
                     RAKTSAVPVVVFSMKELRNGVLLNILKLNNIDLIVLAG"
BASE COUNT         5168 a         4372 c         4179 g         5063 t
ORIGIN      
        1 atttgaacct ttttcaccga ttcgttaagc tggtaaactg cctgctgtac ctggagagaa
       61 atgagctctt ttgcctcatc cattttgagt tcggcaacgc gttgctcgct atgagccgct
      121 ttaagcgtgt gccccctgtc gttccagtga aaaattggta tattcagcac tacacctacg
      181 ttccagtcgc caccaaaatt ttcggaaaat ccattatagg ggtttgggtt gaaaagcatg
      241 tagtttgccg aaagcccaat gttgggcatg aagcgcgaac gcatcaggtt gacacccgat
      301 ttggcaatgt tgatggtttg gtttagcatt tctatttctg gtcgtttcgc caaagccgta
      361 tcggtaaaac tttgcatgat aacggctgta tccatggagg ctaccaacga gtcggccaaa
      421 aggatttcct ggttcagggg aaggcctacc tgctggcaaa gggccatttt agaaagggca
      481 aggccgttct gcacctttac caggtttagc tgagcttcat ttagtttcac ctttaccttg
      541 agcaggtcgt tttgaataac cattccctcc tcgtaaaagt tttcaatatc aacgctaagc
      601 ttctctagaa gctgaatgga ggcatttacc agctttacct tctccccaac tgctaccacc
      661 cgccagtagg cctcttcagt tttgtagatg atatcggagc gctcggctgc ctcgttggca
      721 cgtgccagac tttctccgta cttggcaatc cggtatgttt ccctaatttt tccaccggta
      781 aatatgggtt gggtcaaact tactccggcg gtgtaaacat tcttatagcc tacttcggcc
      841 tgatcttttg gaagccatgt gtagttttga aaaatcggat tgccattggc atcggttacc
      901 ggctgaagcg ttgaggggtt tattacaaaa gtattcgccg gatcgagatt tgggttaaag
      961 ctgcccgtac tcgggtcgat tgcgctgtag gggattaccg gaaggaacaa gtcgttctca
     1021 aacaagctga actttttgtt ggtgcgcatg tacaaaccat ttgcacttac actcggaaag
     1081 aactgggttt tggccgaact tttcagcgag gttgctgcct gtacatgctc accagcaatg
     1141 agcatccgct ggttatgctc caatgccatt tggcggcatt gctcaaccgt tagcagctgc
     1201 tgggcgttgg acactccgtc cgctagcacc agcaacacaa aaatatacag aagccgtttc
     1261 atccattcag gtatttatag tattaataaa cagttcgttg taatattgca catttatagc
     1321 tgaagtcgtt cacacaaaaa gtgatcaacc atttcgggtc tgctgttcat tcctttttgt
     1381 ttgttattgc tacgaacgtc acaacagttt acattttcca accacacatg aatacgtcac
     1441 gttaaatgcc atcaacacac ccggttaacg tttaacgatg ccgaagaaaa gaatatccag
     1501 gaggtcgtca ataaccgggt tcatggcatt ctcatcgttt gtagagttaa cgaggggtag
     1561 ctccaaacct ttcatagccg ttacaattgc cagtgctgac atgtagaaat cttttacctt
     1621 aaaagccccg ctacggctac cctcctcaag aatttgggtg atgatggaaa tttcctcatc
     1681 atcgtatttt ttccgaaatt tctcggttag ctgcaggctt tttaggtcgt cgttcagcct
     1741 ctgttccacg aaactcgaca ggtgccgtat aaactccata cgtagcttaa cgtacgagcg
     1801 catttttgat actgcatcat cggggctttc gagtattttc tttacatttt ctctttgttg
     1861 gcttgcttcg cgtgcaacaa ccgccctgaa aatctcctcc tttcccttaa agtagtagta
     1921 gattgaactt ttgcccattc tcaagctacg ggcaatatcg tccatggagg ttcttctaaa
     1981 tccaaagcgg ctgaacgcct ttgtggcggc attgataatg ctattccgca tttcatcatg
     2041 cgggctgcca atcacttcct gatgcatggt taaatattcg actgttttgt taaaacgttc
     2101 caaaattact acgtaattat tttttgtcaa ggaatattcg actaaaaagt gttaaaattc
     2161 gaataaatgt tttatgttgc tgataacctt tgagttattc gatagtttta gaaatgttta
     2221 ttggaaagcg aatagaagga agcatgaagc gataactgct aaccgggtat agcttaattg
     2281 cagcgtttaa cctgtagagt agggaatccg cgggcaggca aacgagtctt ggaattactt
     2341 tatcctttaa ccctaaacct ttatccttta ttttccctcc ttccctcttt cagaaagatt
     2401 tgctaaaaat tgtaaatttg agggaatttt aaatagttaa attgatacta aatgcttttt
     2461 agcgatatag ttggacagga aaaggtaaaa gaacggctga ttcagctggt aagggagaac
     2521 cgtgtgcccc atgccaacct ttttgtaggc ccacccggtg caggaaaact ccccctggca
     2581 gttgcctttg cacagtatat catgtgtgaa aaccgtggca agagcgattc gtgcggagtt
     2641 tgcccctcat gcaggaaagt agccaagttg gttcaccccg acctgcattt cgttttcccc
     2701 accaagacaa aggaaaaatc ggatgagggt gccgatgaaa attccgaaaa acctgacgat
     2761 accatttcaa catggcgctc ggctattatc gccaatccct actttggcga gaacgactgg
     2821 tacgaaatga tcggaatgga gaataagcag ggaattataa gcaccgccac tgccagtgaa
     2881 ataatcagaa aactaggctt gaaaagcttt gaaaacgagt ttaaggttat aataatatgg
     2941 ctgcccgaac gtatgaataa tcaggcagct aataagctac tgaaactgat tgaagaaccg
     3001 cccgaaaaga ctatcttttt gctggtgagc gaaaacccca cccatatcct aaaaaccata
     3061 ctttcaagaa cccagcaggt gatggtacct cccattaatc gtgaaagcgt agcccttgcc
     3121 ctgcatgaca agtataacct ttctcctgaa ttggctcacg acatcagccg gatagccaat
     3181 ggaaattttt atgaggccat caggttgaag gaccaggaat caaactactt tttcaccagc
     3241 taccggaagc taatgcgact ttgctactcc aaaaacgtgc tcggactcct cgactgggtt
     3301 gatgaaacag cttcgctggg cagggaggac ttaaagcacc tgcttgttta ctcccttagg
     3361 ctaacccgcg aaaccctcgt gctcaacatg ggagttgagg aggtttcata cctgatgggg
     3421 gaagaggaag cattttgtca cgatttctca ccattcatta caacaaataa tgtctttagc
     3481 attactgacg agctggttac tgccttcgag tgtatcaccc aaaatggtaa cccaacggta
     3541 gttttaaccc acatgaccat gaggatggtt aagctaatcc ccaataaaaa agcggcctag
     3601 aaggttcata attttttgct aaatttgacg acttttaatt ggctaacctg acgatggaaa
     3661 aaaatatagg gtcgaggggc ggggttataa ccgtgaacga agcttccgag cattcggttt
     3721 gcagctcaaa gggttgcgcc aagctaaaag ttcacgactg gatggccgac cttcccatgc
     3781 cgctccagca atcggatatt attgaagttc aatttaaaaa tacccgaaag ggtttttacc
     3841 gaaatgccaa ccagctcgtt cttcggcaag gtgatattgt ggctgtggag gctacccctg
     3901 gccatgatat aggcgtggtt actcttaccg gctggctggt agagcgccaa atgaaacgag
     3961 taggagttga ccctaaaact accgagttca aaaaggttta ccgtaaggca aaacaggccg
     4021 atattgagaa atggatagaa gctattgccc ttgaaaatag caccatgatt agggcaagga
     4081 aaattgccga ggaacaaagg ctgaacatga aaattggcga tgtagagtac cagggtgata
     4141 aaacaaaagc cattttctac tacattgctg acgaacgggt tgatttccgg gagctaatta
     4201 aaatactggc cgaggaattc agggtaaaaa ttgagatgcg acaaattggt gcccgtcagg
     4261 aggcagggcg tattggcggg ataggcccat gcggtcggga gttgtgttgc tcatcgtgga
     4321 tctcacactt tgcctcggtt agcaccaatt ccgctaaggt tcaggagctg tcattaaatc
     4381 ctcaaaaact tgctggtcag tgcggaaaac ttaaatgctg ccttaactac gagctcgaca
     4441 gctacatgga tgcccgcaag gacttccccg ataccagtat tgctcttgaa accgccgatg
     4501 ccacttacta ccacttcaaa accgatatct tcagacggag tatgtggtac ggcagcgagc
     4561 cttcttcgtc ggttaacctt atcgaagtct ctgttgatcg cgttaacgaa attatcgccc
     4621 taaacagaaa gggaattaaa gccgacaagt tgttggaaaa tactacatcc gaccagaata
     4681 actcccggcc aaacggcttc accgatggtt tactcgacga gggtagcctg acccgatttg
     4741 ataaaaaggg gggaaagacc gcaaaaagga aaaaaagcag aaatcgcaac cgaaccaggc
     4801 caagcaatga gtaaaagcct accaattgct tttttggcag ctacgcttac attggcttgc
     4861 tgcaacaggg atgccgtatt tgattcatac gtagctattc cccgtgaaac ttggagcgcc
     4921 gatagcttgg ctagcttcaa cattgctatt cccgacactt cccaatccta cgacatcata
     4981 attaatctac gcaactccac cgactacccg tttcaaaacc tttttctttt catcagcatt
     5041 tactccccca acggtggctt tgtccgtgat accttcgaat gcttcctggc cgacgataat
     5101 ggtcgctgga cgggtagggg tagcgggaga attcacgaca accggttcat ttaccgcgag
     5161 ggtgtaaagt tttatcaacc tggcagctac agatttgacc ttcagcaggc catgcgagtt
     5221 gaagacctca ggggagttgt tgatgttgga ataagaattg aacaatccca acaaaacttt
     5281 aaataactag ggcacaagct actcagccca tatcacgtaa aaatcataac tctctgcaag
     5341 gtgggtaaga ataagcttaa gcgatttgcc gagaacgaaa cattctccaa cctctttcag
     5401 cccccccttg aggaggtgct taacgacaaa ttcgagctaa aagggaaatg ggggaacagc
     5461 tactttaaaa atatcaaccc tttggttctg gagctgggat gcggcaaagg ggagtacacc
     5521 gttagcctgg ccggtatgaa cccacagaaa aattttatcg gtatcgacat aaaaggggcc
     5581 agaatatggc gcggcgcaaa aaccgcactt gaaaacaagc tgaacaacgt ggcgtttgtt
     5641 cgaacccgca ttgagcatat atgctcaatt tttgctcaaa atgaggtgga tgagctgtgg
     5701 attacctttc ccgaccctca ggaacgagag attaaggcca aaaaacgcct aacgtcgagc
     5761 cgttttttga acctctattc aggattttta aaaccaaatg ccattgttca cctaaaaacg
     5821 gataaccggc agctgttcga ctacaccaag gcgctggtgg aacaaaataa atttaatgtt
     5881 ttaatctgta ctaccgacct ctatgccaat tttaccaacg atcctgttct tggtataaaa
     5941 acattttacg agcagcaatt tctaggtgag ggacttccca taacctacct ccgttttgtc
     6001 cttaaccatc ccggacccta ccatgaacca atccggtaac gataatacag gtttcttcga
     6061 aaaggtttac gaggtaaccc gtatgatacc gtatggcagg ataaccacat atggtgccat
     6121 tgcacgctac cttggttctg ctcaatcggc cagaatggta gggtgggcat taaactcctg
     6181 ccactcccaa cccgattggg ttcctgccca tcgtgtggtc aacaggaatg gccagctaac
     6241 cggaaaagtc catttccctg gttcaaacac catgcagcag ctgctcgaat cggagggtat
     6301 tagggttgag gatgacaccg ttttggagtt caaaaagttc ttttggaatc ccgctaccga
     6361 actttaacct ttaaataacg ttttataacc atggacatcc caaattttat tctgggaggc
     6421 atgacaatgt cctgtaaaat aacctacttt tgtgcttttc taagtataac aaaatcaaca
     6481 taatgaattt gaccaccgat caggttttgg aggtactcaa ccaagtagta catcccgaaa
     6541 gcgggcagag catagtgagc atgggcatgg ttaaggattt gacagccagc gaccggcgca
     6601 tttcatttac cctgaagctc acaaagaaga acgatccctt tgcctcatcg ctcaaaaaga
     6661 cagcccaaag actgctgacc gaaagggttg gacaacaggt agaaatagac atacaggttg
     6721 ttgccgacat ccctgaaaga atatccccac taaaaaccaa agcctcggtt agccgggtaa
     6781 aaaatattgt ggctattgcc tcgggaaaag ggggtgtggg caaatcaacc atagcagcca
     6841 acctggccgt ttcggttgcc aagtcaggcg cccgtgttgg acttatagat gccgatatat
     6901 acggaccctc cattcctaaa atgttcaacg tagaaaacgt tcaacccgaa gttatgaagg
     6961 aggatgagat ggaactgatt gttcccgttg agcgttacgg cgtgaagctt ctctcaattg
     7021 gttttttcgt tgaccccaac aatgctctta tttggcgggg acccatggcc accagcgcac
     7081 taaagcagct aatccaccag ggagcctggg gtgagctaga ctacctgttc attgacatgc
     7141 cacccggcac cagcgacatt cacctgaccc tggttcagga agttgcggtt accggtgccg
     7201 ttattgttag caccccccag gacgttgctt tggctgacgc tgttaagggg ataagcatgt
     7261 ttggaagtca ggggataaat gtcccagtgc ttgggctggt ggagaacatg acatggttca
     7321 ctcccgaaga actacccgag aataggtact acatttttgg aaaggatggt tgcaaaaagc
     7381 tggctgagcg catgaaacta ccgttgctag ggcaaatccc cctggtgcaa agcattcgtg
     7441 agggtggcga tttcggtgaa cccgttgccc tgcactcatc ggtggtggcc aaagcatttg
     7501 atgagcttgc cacaaatctt attagacagg tcgaaattcg caacagggaa cttcctccta
     7561 ctaccattgt aaacgttaaa cactaataaa ttatccattt caaaattttt aaaccatgac
     7621 tagcaaggag gaaattgtaa caaaagtagc tgcagccatt gatgttattc gcccgtttct
     7681 tcagaatgat ggcggtgaca tctcactggt agaagtaacc gatgacaata cggttagggt
     7741 gaagctgcat ggcgcatgcg gtagctgccc ctacagcctg atgactttga agaatggagt
     7801 agaacaagcc atcatgcgcg atgttcccga aataagaggg gttgaagcag ttaacctaaa
     7861 cataccctaa caactttttt tcaagtatcc taaccattta gaattctaga acttcaggta
     7921 acggaaaact ccctttttaa agggagtttt tttgtagtat aaaatatgtg gttggcaatc
     7981 ataaaattat aatcccatca actttttccc cgggtctgtc aaaaatcaaa cgcaaaccct
     8041 tgcaaaagca aagttcccca tcgacattta catcaaatta tcattaatac tttcaaccta
     8101 tgagatacgt actacttttt accctgatgc tggttgttgc ctcagttgct gcgcaggaac
     8161 cagtaacgct taaatttacg cgtccatcaa aggtgcaggc ttcagcatca aaaatcaagc
     8221 tgaaagtcca aggtaacgaa tacgttctta agaatggagc aagcatatcc atcaacgtta
     8281 ccccggagta cggaaagtcg ttgctgatta atgccggaga ccaaactgcc tactacttga
     8341 aacctaaacc tggggaaacc tacgagtttg aggttggttt taaatttgca ggcccttaca
     8401 tactgctgct gagcggggaa gaggccaaac caggcgaggc tgtccaggta accgacacca
     8461 ctgccaaacc cgaggggtgg gaaaccaacc taaaggttga ccgggataac atgggagctg
     8521 gaattaccct tcaaaaaacc gaccagtccg aggcaataag gcaggagtgg ctggctcgcg
     8581 ggggtaaaat taaataccaa tcggttgttt tcaacggaac ctattttggt atggacatgg
     8641 acatggaggg cgttgacgca aacaggatag acgggtacgg aggcggcatt tcgttctacc
     8701 agaacaggat taaccttaaa attcctgcct acaaaaccgg agcttcatca tggaactcct
     8761 ttaacatcgg ttttggatac gacatgctgg tgtacgcata caaatttgac atgaagatgg
     8821 accccataac aactaaaatg gatgtggtta ctattaccat gccattctcg gccaatattg
     8881 gatggacact gggtttggga aagtttattg atgaggggaa ctggaaggga gttgccctta
     8941 ccctaaggta ccgtccttcc attatgttta actttaccaa ctcaaccgtt aagatgacct
     9001 caaccgatcc ccacatctcc ggctctactt ccaccagcac cgacagcaat gtgcagttta
     9061 acgctggtgg tgtgggcttc gacgttgatt tctcaaactt tagctccacc atggagaagc
     9121 tcgccccgaa acccaagctt aaactttcgt tcttcgtcat gccccccgtt aaggacatgc
     9181 ccctgtttat cagctttggg ctgggtataa ctacatactc ccgctagtaa tttataaatg
     9241 aaaacacacc caaaagttat cagtatgctg attacaagct aaaggtcata ccccgataat
     9301 ctaaaagtct tgggccaaac ccgacatcag aactataatt acaatagaaa taaacagtgg
     9361 agtcctaaaa ccactataac caagaggggc gttaccgaaa agccaaacga gtaggaaaaa
     9421 attcaactta actctaattt tatgagaaca ctaggaaaca ttttatggca ttttcctttt
     9481 ttggggtttt taaccgcact tggaaccttt ttaataggcg gtttgctggt tataactgta
     9541 attggctcac cacttggtct ggggctaatt cagctttcca agttcttgct tgccccattc
     9601 tccaacgcca tggttgataa aaccgacctt caggccgatc aaaataaggc atggaagact
     9661 tacgggatta ttgtccggat tctttacttc ccgattgggc tatttctggt tgttgtaaac
     9721 atttttcaaa ttgccggcct atttatttca atcgttggga taccagtggc tctggttttg
     9781 gcaaaatcaa ttggtactta cttcaacccg gtaaacaagg tttgcgtttc aaaagcagtt
     9841 gccgatgagc ttagctcaag aaaggctaag gcagaggttg ataaggcttt tgcaacaaag
     9901 taaagaatgt agcataacat tccatccaac aaatttaaaa aaggcatttt aaatgcccta
     9961 tcctccttgg cttgttacag catcccaggg aggtttcttt tttgtaaaag catagtgaaa
    10021 ataaaggccg ttttgataaa agtttactat aaatacattt atatttacag aataaagccc
    10081 aaaaatatgg tcaaaaaaat cttcatgctg ctacttgcta ccctaatgtg tccggcacta
    10141 tacgccatac ccgacacgtc aagggttgcg ttgctggtta tagatattca ggatttctac
    10201 tttccgggtg gcaaatcgga gctggtaaac ccgttgcctg cggctaagaa tgcagcgctg
    10261 ctggttgaat acttccgatc gcaccagctg ccggttatat acgtaaaaca ccagtcggca
    10321 gaacaggaag aaattaacga gatggtaaag cctctggccg atgagccgat tttcaccaaa
    10381 acggaggtga actgctttgt ggggacacaa ctttacgact accttgtggg ccagcaaatt
    10441 tcaaagctgg ttatctgtgg tatgcaaacc cacctctgtg ttgaggcagc agttagggct
    10501 gcttcggact atgggttcaa ggtaaccctt attcacgatg cgtgtgccac cagggatgtt
    10561 aaatggggca atactaccat tccggccaac atggttcacc tgtcaaccct ttcaaccctt
    10621 caaaactacg ccagaatact tagtaccgag gaatgcctca aggccaaccc ttaaagattt
    10681 aaccacttaa aacgcaggac tggcaaaagc tttaacatcg cctccggtta tgtccttgtc
    10741 gcatagtatg ataagccgtt cgacaacatt ccgaagttcc cttacgttcc cggtccaggg
    10801 tagccgtttc aactcgtcaa cagcatcggg tctaaacacc ttaggcggta tgccatactc
    10861 ggagcaaatt agctggttga aatgttctgc aagcaatgga atatcctcaa ccctttcgct
    10921 tagcggggga acatgaatga tgatgacgct taaacggtgg taaaggtctt cgcgaaagtt
    10981 accattttga atctcgtggg tgaggttttt attggttgcc gcaataaccc tcacgttaac
    11041 gggaatatcc ttatcgctcc ccacacggct aatcttgttc tcctgaagag ctctgagcac
    11101 ttttgattga gcgcttagac tcatgtcgcc tatttcatcg aggaaaagcg ttccgccatc
    11161 ggcctgttca aatttaccct tgcgctgctt gattgccgat gtaaacgctc ccttctcgtg
    11221 tccaaaaagt tcgctctcga taagctccga tggaatggca gcacagttca cctccacaaa
    11281 aggcatgtcg gagcggttgc tcttttcgtg aagccatcgg gctaccagct ccttccctgt
    11341 tccgtttggt ccggtgatga gcacccttgc ctcggttggg gcaaccctgt ctatcatctc
    11401 cttgacctta acgatagccg gcgactcgcc aactatgtcg taggccttat tcaccttgcg
    11461 cttaaggatt ttggcctcct gaataagggt agttttatcg gttgcgttgc gtatggtgat
    11521 tagcaaacgg ttcaggtcca acggtttctc aataaagtcg tatgccccct tctttataga
    11581 ctccacagca gtgtcaatgt tcccatgtcc ggatatcata accaccggta catcggctgc
    11641 ttgttcctga agcttttcaa ggacctcaat gccatccatc tggggcattt taatgtcgca
    11701 gagaacaata tcgtagcatc cggagccaaa catctcaatt ccggttgggc catcttcagc
    11761 caaatcaacc gaatggcttt cgaattcaag tatctccttc agggtgcttc ggatagcctt
    11821 ctcgtcgtca atgacaagga ttttggccat agctcaattt ttggtaaata taccaaataa
    11881 tgcttaatga acgtgattaa aaagcatggc tgctttgcag ctcggccata aatccctcct
    11941 taagggcgaa tgatgactgg aacaacttgt taattcctat tttttgaatg atgaggttaa
    12001 taaataccga ggccggaaca atcaggtcaa cccgcatggg gtccagtccc tttagcgcca
    12061 tgcgctgatc gtgggtggag gtgataaaaa aattgtgcag ctcgaagtac cgttcaaggg
    12121 gtatttcggc ccagggttcg ctaccaggag caatgcttcc cgagtgctcc atgatgctac
    12181 gaattgaatc gaaagagccg gagcttccca ccaatcggac aggaacatac ctacgcgcag
    12241 catcaaaaag agtttgaact tccacattaa taaactcttc cagagccctc agctgcttct
    12301 gtgttatagg atcggaaagt tgaaactgct gcatcaacct tgaaacaccc aggttaaagc
    12361 tgtgaagcca aaatggctcg ctggtattcc caagtattat ctcattactt ccaccaccaa
    12421 tatccagaat caagctacag gtgttaccca aatctaatgc ctccctgacc cccttgtaca
    12481 ccagcgttgc ctcgcgatta ccgtcaatta tttcaacatt gagcccaaaa ctggtgcgaa
    12541 tatcctcaac aaagtttgtc ccgtttgagg catcccgggt agccgaagtg gcaaatgcaa
    12601 agaacttacc agcacccaag cttttcgctt tagccatgtg ggtattaatt gcatccaggc
    12661 cacgctgcca tgcctccgga gtaataatct tcctgtttaa accgccctcg cccagcctaa
    12721 ccgggagctt ttcctcgtaa ataatctcgg ggcgttcttc ctggttaaac ctggctacca
    12781 acaggttgaa ggtgttggtt cccatgtcaa taattgccac attcataacc taaacatcta
    12841 atctaaaaca ctccactcct taaaaaactg cgtaagaaac agctccataa actgatgcct
    12901 ttcaacagca atttgccttg ccgtttgggt gttgagccta tctttcaaaa gaagaagctt
    12961 ttcgtaaaag tgattgatgg tgcttccgtg ggcatgttgg tactcatcaa acgagtggtg
    13021 ttgctggggc ttcaaatcag gaatgtacat gggccgttgc ctggctccgc cgtaggcaaa
    13081 tgcccttgca actccaatgg cgccgatggc atcgagccgg tcggcatcct gaacaacctt
    13141 tgcctcaata gtggaaggag tagtatcaac accggcaccc ttaaatgata cctgcccaat
    13201 gataagtgca acacgccggg cttcatcatc gctatatccg gattcgtgca gtattctgag
    13261 cgcaacaggg caaccctcgg tgtttctgtt cagcttccaa tcgtcaacat cgtgtagcaa
    13321 agcggccagc tcaaccgtga agggattggc attttccctt tctgatatga acaacgccag
    13381 cttaaccacc ctgagaatgt gccaccagtc gtgacctgtt gaatcgcccc ggcaggcaaa
    13441 tttaacagat tggtaaacct gttgtaaatc gttatccatt ggggtaatac aagtttgtaa
    13501 aaaaagcgct tcaaaatgcg aagagcgaaa atccgatgga ttattcgccc tcagctcaac
    13561 aagcttatta cctgctgcta gcttctatac ctaatccact ttatcatctc cttccacgag
    13621 tacttcttgc cgtacatcag aattcccacc ctgtaaattt ttgcagctaa ccatacagta
    13681 acaacaaaag ttccgattag tagcgctgcc gaaaggataa gctgccatac aggaacgcca
    13741 aatggaagcc gggttagcat cactatgggg gaggtgaaag gtatcatcga gaaccagaag
    13801 gcaagggaac cgtcgggcga tttaatgcca acaatcataa caaatatcgc cagtataagg
    13861 ggaatggtta tgggcataac cagctgctgg gtgtcggaaa cattttctac agccgaacca
    13921 acagcagcgt acatggcggc atagagcagg taccccatca ggaagaagaa tataaaaccg
    13981 ccgattatca atccatagtt ctgatttttc agggcttgta gaaagttatc ggtatttgtt
    14041 tcagagattg ctgctgttac atcggctgac tgagctcctt ggttggccat gatggactgg
    14101 ttggcaattt gaacctgctc catattggcc ttgcctgatt tttccgatat gaggttaagg
    14161 ccaactccgg ccaaaccggc agtgagtaca atccaaattg caatctgcag caatcccacc
    14221 gctgctattc caaatatttt gcccatcata agctgtacgg gctttacaga cgaaacgagt
    14281 agctcaacaa cgcggttatt cttctcctca attaccccct gcataacctg gttgcccatc
    14341 agcatcacca tcatgtacat caggaaactt aaaatataag ctattcccat ggttatcagg
    14401 gtattgctct ccttttcctc gccatccttt gaaattttga tggagcggac gttaacctta
    14461 gtttttacgt ccttgagtat tttatcaagg tcctgaatgt tgtaggtcat caacttacgg
    14521 gtttcaattt ccttctccat ggagttctcg atatgcgaaa tcacctcaat ggaaggttgt
    14581 ttgggcgagt agatggtaag ggctggggca atgctacccg gaagtgaatc aattgtaagc
    14641 agggcgtagt agcctgactc ctctagtgaa ttttttacct tttcggccgg cgtatcggtt
    14701 atggtagtaa ttttaatgta ttcggtttgg ggaatagcac ttccaaagtg ctgtgtattg
    14761 tcaataacgg caatattgcg cacttcagtg tcctccatgg tggcaaaata ggtaggaaga
    14821 attaccatgg cggcaaaaaa tagcggcgaa agcagggtgg taaaaatgaa tgattttttc
    14881 cgcacccggg tcataaactc ccgctggagt ataaggaaca tttgtttcat aattccggaa
    14941 ttttttataa aatactagtt aagcgataaa ccatgagcct tgttgtactc ctgcacaacc
    15001 cgaataaaga tatcgtgcat gctgggtact acgggattaa acgaaagcac ctcaacactt
    15061 gaaatcaggc tttgaagcag cttgttgcta tccgaagccg attgcagcct tacctgcacc
    15121 cttggaatag ccttctcggt gttaacgtta aaaatgtcaa atgtaccgtt caggttttgc
    15181 ctcagggttt cgaggtttcc cttaaaggca acttcaaata cgttgctgcc atagttagcc
    15241 cgcacctggt caatttgccc gctaaggata ttttttgatt tattgatgag ggtaatgtga
    15301 tcgcaaagct gctcaaccga ttccatgtta tgggtggaaa agataatggt ggtgccattt
    15361 ttcttcaact ccagtatctc atcggtaatc agctgggtgt ttatagggtc gaaaccgctg
    15421 aatggctcgt cgaatatgag cagctcgggt ttgtggataa cggttacaat gaactgcacc
    15481 ttctgggcca taccctttga gagctcctca atttttttat cccaccagga ttgaatttca
    15541 aatttttcaa accagtactt caggctttta gttgcatctt gccggcttaa ccccttgagc
    15601 tgggaaaaga atagggcttg ctctccaacc ttcatttttt tataaagacc acgctcttcg
    15661 ggcaaatatc ctatccggta gatgtcgttg ggttcaattt tgcggccatc gaacataact
    15721 tctcccttgt cggggccggt aatctggtta attattctga taagtgttgt ttttccagcg
    15781 ccgtttgggc caaggagtcc atagatagat ccctttggaa tggcaatgct gatatcgtct
    15841 agcgccaggt ggtttgaaaa tcgcttctcc accccatgag tctctaaaag attcataaat
    15901 ctgatgggtt taagttcacc tatgaataaa attatgcaaa tgtagcaaaa gcaactaata
    15961 atccttacaa taaatactta atcagcacct ttgaagaatg cgctcttgca tgcagcgcag
    16021 aggtattagt agaaggaaga ggtaacgaaa atgagcctaa actgtgggtt tgtcagcccc
    16081 ctctgtaata gtctccattg cctgcatagc ggcattctgc tcggcctctt ttttagaagt
    16141 accatggcca tgggctattt ccttgccatc ccagcgaaga acagaggtaa aatgggggat
    16201 tgccgactcg ggcagtgact ctattttagt taaaaattcg gcagaatttt tttgcttctg
    16261 ggccagctcc agcagcctgc ttttaaaatc aatctcaaca tgctgaatgg cttcaaggtc
    16321 aacctgacgt tcgagaatat tactaaccac taacctactg gtttccttga atcccatgtc
    16381 gagaaacatg gcgccaataa ttgcctcaag ggcattacca aaaatatttt tttggttgaa
    16441 cgaggcaccg tttcccagca ctacgatatt tggtaaacca attcctatgg ctagctggtt
    16501 aaggctagct cggctaacaa ttttggcccg tgtttgagta agaaaaccct cattttggct
    16561 ggggaatcgg gtaaaaaggt aatcggctac gatagcatcg agaatggcgt cgccaagata
    16621 ttcgagccgt tcgttatcaa ccttctttcc atgagcaata actaacgagg cagacctgtg
    16681 aaccaaggcc aacttataga gttctaggtt ttgaggtgaa aatcccagta cctgctcaat
    16741 actacggtag atttcatggt ctgcagcagg aactctcaga cgccaaaatg cttttggaag
    16801 gaatttccgc accggtagct attaaatctt ctgaaaaatt atagaagcat tgtgcccacc
    16861 gaatccgaat gtattgctaa gcgctgcctt aacgcttctc ttctgggcgc tgttgggtgt
    16921 aatgtttagg cggctgtcaa tttccggatc gggtgattta aagttaatag tgggtggaat
    16981 ggtatcggtg cagatagcga aaatagcagc aatagcctca atggcaccgg cagccccaag
    17041 taggtgaccg gtcatggatt ttgtggagct gatattgagc ttgaaggcat aatcgccgaa
    17101 taccgaaatt atagcctttg cctcggcaat atcgccaaga ggtgtggcgg tgccgtgtac
    17161 gttgatgtag tcaatatcct tgatagtaag gccagcatct ttcagggcat tttccataac
    17221 ctttcgggcg ccaagtcctt cggggtgcgg agctgtgaga tggtaggcat cagccgacat
    17281 gccaccgccc tttaactcgc aataaatttt tgcacctctg gcctttgcgt gctcgtactc
    17341 ctcaagaatg aggcaaccac caccctcacc aattacaaaa ccatcgcgcg tggcgtcgaa
    17401 tgggcgagat gctgttttgt actcatcgtt gttgacagat atagcctgca tggagttaaa
    17461 gccacctacg ccaacttcgt tgacggatgc ttccgaccca ccagcaatga taacacttgc
    17521 tttcccggtc cgaattatgt cgagtgccga aatgatcccg ttggtagagg atgcacaggc
    17581 tgaaacggtg gaaaagttag ggccgcgaaa tccgtacttt attgaaatgt gcccggcggc
    17641 aatgtccgaa atcatcctgg gaataaagta agggctaaat cggggtgttc catcacccgc
    17701 gaaatagcct cttagctctt cggtgaaagt ttgcattcca ccaatccccg atgcccaaac
    17761 aactcccgcc atatcaagat caatagcttc agtatttagc ccagaatcct tcatggcctc
    17821 ctcaactgcc accagcgcaa actgagtgta agggtctatc ttttttagct ccttacggtc
    17881 aaaataggca ttgggatcaa aattctttac ctgacaggca aatttggttt taaactttga
    17941 ggcgtcgaaa ctggtaatta gctcgcttcc gctcacaccc ttttcgaggt tagcccaata
    18001 ctccggcaca ctgttgccaa taggatttat tgttcccagg ccggtaacaa caacacgctt
    18061 catttccata aattagaaat gtttagttta aaccaattgc ctataaggat tttacaaatt
    18121 acttgctgtg gccttcaatg tagctaattg catcgccaac tgtgccaatc ttttcagcct
    18181 cgtcatcagg aatcgagagg ttaaactcct tttcgaactc catgataagc tcaacggtgt
    18241 caagagaatc ggcaccaaga tcattggtga agctagcctc gggggtaact tcattctcgt
    18301 caactcccag cttgtcaaca ataatcgatt taactctggt tgcaatatcc gacatagctt
    18361 taaaatttag ttagtaaata aagttttcat ttgcaaagta aaagaacttt tcacctgata
    18421 caaaaccaga tgaacacttt tcagccgcaa aagtaatttt tttgttttta catttgccaa
    18481 atccttttaa aaaaatttga caatcgttta attttataac gcatagtgct gtttatgaac
    18541 aatatagcaa tactggcttc gggatcgggc accaatacag aaaacatcat ccgctacttc
    18601 agcaatcatc cacttattag ggtaacgtta gtggcaaccg acaaccctaa tgctttcgta
    18661 atagagcgtg caaaaacgtc ggcggttccg gttgtggtgt tttccatgaa ggagctccgt
    18721 aacggagtgc tgcttaatat tctgaaattg aacaatatag acttaattgt actggctggt
    18781 tt
//
LOCUS       DAOPHX010000039        18775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_3229_length_18775_cov_5.414850, whole genome shotgun sequence.
ACCESSION   DAOPHX010000039 DAOPHX010000000
VERSION     DAOPHX010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 18775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18775
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3229_length_18775_cov_5.414850"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..344)
                     /locus_tag="PK502_04500"
     CDS             complement(51..344)
                     /locus_tag="PK502_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00110.1"
                     /translation="MKAVFIAYNQALSDRMDNALKVAGIKGFSKWTEMLGVGSNTGEP
                     HLGTHTWPSMNSGILTIVEDEKVDPLLNYIRQINKIAEEQGIHAFVWDIERMV"
     gene            complement(427..3555)
                     /locus_tag="PK502_04505"
     CDS             complement(427..3555)
                     /locus_tag="PK502_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPH00111.1"
                     /translation="MSLYSTAVNKPVSTIMIFIAIIVLGIFSITYIPVDLMPDMDIPM
                     ISVITTYPGANAEDIEINVTKPMENQLNSLQNLKKLTSSSKDNVSYVACEFEYGINMD
                     EASNNVRDAVSLAENALPDDCEKPMILKFNSSMVPVLIYGIKADKSYAALEKIIDERI
                     VERLSRIDGVGTVTIMGKPIRIINVAVDPRKLDSYNLSLEQVGAAIGAENMMLPAGRI
                     EVGKMEFPVRTNGDFSSSDVVKDLVISNNAGKVVYLKDVATVKDTLKKILVDETIDGQ
                     KGVRLMVQKQSGANTVKIAKMVRSRMEEIKTELPSDVQIVKIMDSSEFIQGSINNLSE
                     TLIYAFIFVVLVVILSLGQWRSTFIILTTLPVSLISGFIYLYATGNSINIISLSAMSI
                     AIGMVVDDAIVVLENITKHIERGSTPREASIFATREVGLAVIASTLVIIAVFFPLTLV
                     GGLTGVFFRQLGWIVTITITVSVIAALSLTPMLSSRMLKSKSHYKHKRNSFVKIVNKF
                     WGFFDEIYGRTLKAILNHKTLTLTGAFIIFVGSLFLMKFIGTEFTPSQDNARISVYME
                     VPQGLRIDETKQLARDFEKKIKAKYPEIELISTSIGADEGGSLMSIFQKSASYIINYT
                     LRLSSIDNRTRTIFEIADSMRRDVAAYPQITTSYVDAGGTRNAGGSAMGMGGGNNVEV
                     KILGYDISQTNQIAEEIATTLKNEKGFKDVSISRDQDRMELQVIPDREKLAQLGLNTT
                     MLASAVRNRVYGFTATKYREEGTEYDVIVKYDDDNLTSISDLENITFKGPQGNLVRVS
                     DVAQVRQVYTTPNIDHENKTRIVKVSSSLTGIDLGKATGIINAKLSTMTVPQNVLVEI
                     GGAAQDMMDTFYDLALLFVLIMLLTYIVMATQFESLREPFIIMFSVPFAITGVLIALF
                     ITGTTLNMVSGIALVMLVGIAVKNSIVLVDFTNLLVDRGYSISRAVVEGGKSRLRPIL
                     MTSATTLLGMLPMALLKGEGSEIWRPLGIAIIGGLLFSMLISLIIVPVIYTLFANARI
                     KRERKSLQEANM"
     gene            complement(3594..4628)
                     /locus_tag="PK502_04510"
     CDS             complement(3594..4628)
                     /locus_tag="PK502_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPH00112.1"
                     /translation="MKTARTLLILSFSALIASCTGNKTEVTGETKQAEVRVSTQPVEI
                     QKVEHQLRLPATVQAYKENHLAPATAGRVEKVLVDVGDRVRAGQVVAILDRTSYQQAK
                     IQFDKLSIDMARMDSLLKVGAVPQQQYDQVKMQYDIAKTNLQFLDDNTTLKSPIDGVI
                     TGKYINDGEMFAMTPVAGIGKAAIVSIMQLDRLKLLVGVSSSYLSKIELSSSVLITTE
                     VFPGEKFNGKIEKIYPTIDNLTKTFTIEVVVPNRSNMLRPGMFATAFITIGSGNSILI
                     PGYVIQKQSGTNERYVFTYSDGKAVRKVIEVGEVFDDKIEITSGLNPGDELIVVGQAN
                     LMDGQAVTKI"
     gene            complement(4655..>5407)
                     /locus_tag="PK502_04515"
     CDS             complement(4655..>5407)
                     /locus_tag="PK502_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPH00113.1"
                     /translation="IKVQVDNLYPTLMQTKNTYNNLLNIFKVLLNVNDTIPVTLDHSE
                     LSTVTLEEIASPTEVWQQDNTTLRQLELSRKMIGIQEKMVRSSSLPSLVAMGNYQYQA
                     QANDFKFNDYRWVPTSLVGLQLNIPIFSGFAVRKQLNQVKISAQQLDMQMEFTRSNLS
                     AQVQNSVNAINTAIKKVESAQGNVTLAQKGYAIAQTRYNTGQATLLELNDADNAMMQA
                     RLNLIQAQFEYLNARNEYEKITGQSALPTPAN"
     assembly_gap    5408..5507
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5508..5863)
                     /locus_tag="PK502_04520"
     CDS             complement(<5508..5863)
                     /locus_tag="PK502_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00114.1"
                     /translation="MRKTIFHVLYGLGVAMAVSLTPLSGYGQTDTLKLTVKDAVNMAI
                     QQNLMQKVSELELKKKEEKVGEYYAALYPNIKATGSYSRYLELPVIFIPEGTFGPGSG
                     GAMKIGYDNSYSGGFSA"
     gene            complement(5890..6513)
                     /locus_tag="PK502_04525"
     CDS             complement(5890..6513)
                     /locus_tag="PK502_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPH00115.1"
                     /translation="MIVKEQIVNQAMLLFRQMGIRSVTMDMIAEQTGISKRTLYENFS
                     NKDELVEACVMQEIESRKEQAKRIIEGSSHIIETYILFMWDHINRLRGTSPLFIKDIQ
                     KLYPQSICKQTNEFNNRMRENMKQFIIMGIKDGVFRKEINPDIASILIFEQIKVLHVE
                     GGFETSPLSMFSPAEVFEHISINFIRGISTSKGLELVDFYYSKHKMV"
     gene            complement(6862..8301)
                     /locus_tag="PK502_04530"
     CDS             complement(6862..8301)
                     /locus_tag="PK502_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPH00116.1"
                     /translation="MRLHVLSRYIGFVLLLNAAFILFSLLLSIHYHDSGFYPLLFTLL
                     LTTLWAVFPMIFVPKTQSLSNKEGYLVVVLSWIISCFIGMLPLLLYGGEFTVVKAFFE
                     SVSGYTTTGASVLKDVEALPKSLLFWRSSMHWIGGIGIVVFMLVILPSLGKAQLTLSR
                     MEISPLAQENFRFRTRKMLNVILYVYIGLTLCDIILLRIFGMNLFDAVTHAFATIATG
                     GFSTKNTSIAWFNSVPIEMVTMFFMFVSGIHFGLLYSTFTFKKSNVFRSPIVKYYFWV
                     TIAGIVAVAINLHGSTYDTWGECFRFASFQVISLSTTTGFANADSSIWPPLSIVIIIF
                     FTIQCACAGSTAGGLKVDRVVIFFKALKSQVLKIEHPQAVIPVRVGNRNISDEVVGSV
                     TLFIVFYILILLASTMLLAAMGVDLLTGFSASVTTMGNAGPGFGLVGSMSNFSTIPEP
                     GLVVLSLDMLLGRLEIFGFILLFLVRSWR"
     gene            complement(8305..9054)
                     /locus_tag="PK502_04535"
     CDS             complement(8305..9054)
                     /locus_tag="PK502_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HPH00117.1"
                     /translation="MSIRLKILSGFIILASLLIISGLISIYELTKLGNSVNKLLRDNY
                     LSIDYARQMSHSLEKQNSAILLAVAGDKGSASDLFGISVKEFKENFEKAATNLTQPDE
                     KTLTDSISLVFNSYRQATESFIASSNASLNEYITTIRPMMETIQNSVDNLLLINQQAL
                     TETAAILKNSPYRTIMPGLIVIITSVVFSMLFYYMISYYFVKPVIGITKGINDFIKYK
                     RPFEVAVDTKDEVNELKEAVKNLTTLKNTPR"
     gene            9191..9760
                     /locus_tag="PK502_04540"
     CDS             9191..9760
                     /locus_tag="PK502_04540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPH00118.1"
                     /translation="MDDKDLVGRVIESNDHKAFAQLVERYQKMVVTTCRGFVQSYADA
                     EDLAQDVFIELFESLPGFRNESKLSTWIYRIAVNKSLNFVRRRKREVLLNNLYFWNKD
                     SNSLALGQAVSPEGEQAMLDAERSKQLKLAINKLAENQRVALILSRYQELSYKEIAEV
                     MGVSVSSVESLLFRAKENLRKMLLKGKNI"
     gene            9807..10265
                     /locus_tag="PK502_04545"
     CDS             9807..10265
                     /locus_tag="PK502_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPH00119.1"
                     /translation="MKMNCKLAQQNMLNYLDGTIDKSIINAFEEHVGSCSSCRDELQL
                     LKPIYKQVEGDLGEYAPNPYFAAKVLAKLTEKREKPTTIFTQKSYILAVSLAAAAVAV
                     GLFVGIQFVSSGSSLAQPEIATILANDYSNFSDSNPYNFEVEEVDTSLNK"
     gene            10272..10820
                     /locus_tag="PK502_04550"
     CDS             10272..10820
                     /locus_tag="PK502_04550"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HPH00120.1"
                     /translation="MNFLTKNRILVIVVVILSLLLLSILGGLGYGYYRSKQASKLPPP
                     ANRFEHQAAFISRSLNFSPIQNEQFIRLGEGFHFKFDSLASQIRLVSGEINNEITRAE
                     VDTLKLNMLIDRYASLQRQLKELSVRHLMEVRALCNPGQQQQFCRMARQINRRHQQMN
                     RNNSNDRMRGMPPRGHQNQNNF"
     gene            10845..11357
                     /locus_tag="PK502_04555"
     CDS             10845..11357
                     /locus_tag="PK502_04555"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HPH00121.1"
                     /translation="MKRLSILLLGAIMAAGFTANAQNCGKGMGMGMGAPKMNPDSLQS
                     CNIPNLTPDQQKKIYDLRVAHIKAVTPLRNQLAEKYAHLKTLESAEKPDLNAINKTID
                     EIVSIKGQLMKSRAAHRADVSALLTDEQKVFFNAHQGMGNRMHQKGMGAGMHQGMGKN
                     KPMGSCQQMN"
     gene            11537..12397
                     /gene="speB"
                     /locus_tag="PK502_04560"
     CDS             11537..12397
                     /gene="speB"
                     /locus_tag="PK502_04560"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HPH00122.1"
                     /translation="MMIGYGGLPEAFTNYSTSKVAVLPVAYDGTSTWVKGADKGPYAL
                     LEASANMEVYDIETNSEVYRIGIFTDEIMEGDFSSPDQMVSAVRSRTLKLINDGKLVA
                     TIGGEHSVSIGAVQAHARTYDHLTVLQIDAHADMRSSYEGSPYNHACVMARVKELCPV
                     VQVGIRSMDSSEKTNIDPHRVFWAHQLVANNSWMDEAIDLITPNVYITFDLDGFDPSI
                     FPSTGTPEPGGLLWYPTLTFLRKVIESRNLVGFDIVELCPNPLDKSSDFLAAKLLYKI
                     LSYKYLNTVV"
     gene            12430..12627
                     /locus_tag="PK502_04565"
     CDS             12430..12627
                     /locus_tag="PK502_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00123.1"
                     /translation="MNKCKCVKVPPPEVGGKEFKLNAYYEFDYIPPVKDNPSFYKVFS
                     LEDNVSGSFSIKAFNEHFKKY"
     gene            complement(12816..13484)
                     /locus_tag="PK502_04570"
     CDS             complement(12816..13484)
                     /locus_tag="PK502_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPH00124.1"
                     /translation="MYPILSKSPLFMGVSPVEIENMISQTPHVVKNFSKGQLVAQRND
                     EVKSLTILVEGSVKGEMADFSGKILKIEEMQAPKPIAHAFIFGEHNQYPVDVVALDDC
                     RILYIPKADFLALMQRNATVLINYLNSISNRTQFLSNRLWFLSFKTIKEKIAHYLLNL
                     SYGGANATLILPKSQQELAEFFGVTRPALARVFSDLEVEGIISIKRREVVIRDRNKLI
                     EIAS"
     gene            13664..14269
                     /locus_tag="PK502_04575"
     CDS             13664..14269
                     /locus_tag="PK502_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00125.1"
                     /translation="MNDHQTGKLDISFNTDLPEPLVFSTWKHHLNYIHDAILSIEVGS
                     ETTSLINEVAAGVGGTLVDLYIGELTPMVIAEEVIQQLVSFDALGYDSFSRWLGVSKS
                     GYQNMYLSDGSAWILRLGMVNRRYVHIHPARYSLNTVRCRSANLKFAIAYRLLYGMDD
                     SNFSIDKVNRTRETLGLPPAAEKIALSGALRILKQLSVKID"
     gene            14737..16407
                     /locus_tag="PK502_04580"
     CDS             14737..16407
                     /locus_tag="PK502_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPH00126.1"
                     /translation="MRRNSFLYALTFSVICYACGSKERNVTSEQQVFRYNESKGISTL
                     DPAFARNLALIWPVTQLFNGLVQLSDSLAVEPCVAKQWHVSDDGLQYTFLLRDDVYFH
                     DSEVFPNGLGRRVTASDFVYSFSRLLNPKLASPGSWVLSMLDRNFAGFTNGCKALNDT
                     ILIIRLSKPFPAFLGLVSMPYCFVVPHEAIDHYGGEFGRRPVGTGPFYMKYWRDGEKL
                     VLRRNAHYFELDNEGKRLPYLESVVVTFVVDKQSEFIEFISGNIDMLSGVHASSKDEL
                     LTRTGNLNPKYREKVLMLTGPYLNTEYLGVLLDSIGLESLENSALHDLRVRKALGYGF
                     DRNAMMLYLRGNLGFPATRGIVPPAMPGFETSTEGFYYNPDKSRQLLAEAGYPNGNGL
                     PPITISTTDDYLDVCEFIQHQLGEVGFDIRIDVLPGAAYRDMMANSKLSLFRASWVAD
                     YADPENYLALFVSSNFSPLGPNYTHFSSSEYDQLYRKALAEPLYDNRLPYYRAMDSLV
                     IANAAVIPLYYDKVVRFISKDVEGITLNPMNLLELKRVRKSYSGLAPS"
     gene            16442..17299
                     /locus_tag="PK502_04585"
     CDS             16442..17299
                     /locus_tag="PK502_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00127.1"
                     /translation="MIVVADSGATKTDWRIIMEDFSSASFETVGLSPYFNTELDFNDA
                     LTHGFPKELNPNEVSKVFFYGAGCGTQERGSRVESYLHSFFPAAEIFAWSDALGAAHA
                     LLHNDQGIIVILGTGSNISYYDGAKLEAKTPSLGFILGDEGSGAHMGRLLLRDFFYGR
                     MDSVLGKRLSSNYDMDLAHVLNMIYDSPKPSAYLASFVPFLLNNVENPYISDLITRSF
                     EEMYQLHLSCYPNIRSIKIGVTGSVGCLFSSILDRVASQHGFKVYKYVRYPISSLVDY
                     HVNLYHSGR"
     gene            complement(17274..>18775)
                     /locus_tag="PK502_04590"
     CDS             complement(17274..>18775)
                     /locus_tag="PK502_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00128.1"
                     /translation="PDLRQTLSYPYFLQLFIQVYTPEKQNLLNNQHEILLEFLKSKIY
                     FSTLPDEKVDILTEIIRLGNGHRVLKEDIKRSYPIHLKLSGNYFTAYHELLSYGIITE
                     EQVVESTGRYFKYVRITSPQLTGLLIVQDIVNSTGGITEETFRQVEEKFAGTELYPLV
                     QGTLYYTGSRDRNEKVLVKFFELCPNAYTHGQLLREICATLRNSSYLLNVLMPEYTKT
                     RLGRQLLVEQNIDLNHLNGYFTTVLKHYLSNTTDISELFFAQTLLAFNGFITLNGMLA
                     QPNHEATRNVKPPADVSPLVAGAWFSNQLFAEIIMGNGNNTTPIVERATSYYRKFTSY
                     AERAEFSELIVPTLLLLGQHPAVENYLEDISSIAGSHYQVVNYFNRKLFEILSGKNHF
                     SAEEDCQIQQIYQQLNPIRSFPQVIAGEVGRAASYFHTQNLTMAHQCIRNAIELSGIA
                     GYKLVEMKLMNGLARVLSEMREEKQAMLCREYAETLWKCSGFTSPNGIG"
BASE COUNT         4953 a         3978 c         4343 g         5401 t
ORIGIN      
        1 ggctacaccg ttcgatgtag cccttgcttt cttaccggta gatggagttt ttacaccatc
       61 ctttcgatgt cccaaacaaa ggcgtgtatt ccctgctctt cggcaatctt gttgatttgc
      121 ctgatgtagt tgagcaaagg gtcaactttc tcatcctcaa caatggtgag aattccagaa
      181 ttcattgatg gccaggtgtg ggttcccaga tggggttcac cggtattgct gcccacccca
      241 agcatctctg tccatttgct gaatccctta attccggcca ccttgagggc gttgtccatt
      301 ctgtcgctaa gtgcctggtt gtaggctata aaaactgctt tcattatttt cggttttatg
      361 ttcagtttta ggaggagtgc cattgggaat agcccttttg gctactcctc ccgaaggtgt
      421 ttacaattac atgttagctt cctgaagtga ttttcgttcc ctcttaattc tggcatttgc
      481 aaacagggtg tagataaccg gaacgataat cagggagatg agcatggaga ataaaagtcc
      541 accaataatt gcaataccca gcggtctcca aatttccgat ccttctccct taagcagtgc
      601 cattgggagc atacccagaa gcgtagtagc cgaggtcatc agaattgggc gcaacctgga
      661 ttttccacct tccacaaccg cacggctgat ggagtaaccc ctgtcaacca gcaggttggt
      721 gaagtcaacc agcacaatcg agttcttaac ggctataccc acaagcatta cgagtgcaat
      781 tccggaaacc atgttaaggg tggtgccggt gataaagagt gcaatcagca caccggttat
      841 ggcgaacggt acggagaaca tgataataaa gggttcgcga agcgattcaa attgggtggc
      901 catcacgatg taggttagca gcatgattag cacaaacagc agggctaaat cgtagaatgt
      961 atccatcatg tcctgtgctg ctccgccaat ttcaaccagc acattttggg gaacggtcat
     1021 ggtggaaagt ttggcgttta taatgcccgt agctttcccc aggtcaattc cggtaagtga
     1081 gctggaaacc tttacaattc tcgtcttgtt ctcgtggtca atgttggggg tagtgtaaac
     1141 ctgtctaact tgcgccacat cgcttaccct taccaggttt ccctgaggtc ccttaaaggt
     1201 aatgttttcc aggtcgctta ttgaggttag gttatcatcg tcgtacttaa caattacatc
     1261 gtactctgtt ccctcctcac ggtatttggt tgcggtaaaa ccataaaccc tgttacggac
     1321 ggcgctggcc aacattgtag tgttcagccc aagctgggca agtttttctc gatcgggaat
     1381 aacctgaagc tccatacgat cctggtcacg gcttatggaa acatccttga accccttctc
     1441 atttttaagc gtagtggcaa tttcttcggc aatctggttg gtttggctaa tgtcgtagcc
     1501 aagtatttta acctcaacat tattgccacc gcccataccc attgctgagc cacctgcgtt
     1561 tcgggtgcca cctgcatcaa cgtatgaggt ggtaatctgt gggtaggcgg caacatcacg
     1621 cctcatgcta tcggcaattt caaagattgt tcgggttcgg ttatcaatgc tgctcaacct
     1681 caacgtatag ttgatgatgt acgaggctga tttctggaag atgctcatca gcgatccccc
     1741 ctcgtcggca cctatgctgg ttgaaataag ctcaatttca ggatattttg cctttatctt
     1801 tttctcaaaa tcgcgtgcca gctgctttgt ttcgtcaatt ctcaacccct ggggaacctc
     1861 catgtaaacg cttattcggg cgttatcctg tgagggggtg aactcggtgc cgatgaactt
     1921 cattaggaat agactaccca caaatattat gaaagcacca gtcagggtta gagtcttgtg
     1981 gttcaaaata gctttcaggg tgcgaccgta tatctcgtca aagaatcccc aaaatttgtt
     2041 tacaattttg acaaagctgt tgcgtttgtg cttgtagtgg cttttgctct taagcattct
     2101 tgacgagagc attggggtaa gggaaagcgc agcgattacc gaaacggtga tggtaatagt
     2161 tacaatccat cccagctgtc taaagaaaac acctgtaagc ccgccaacta gcgtcagagg
     2221 gaagaaaacc gctataataa ccagggtgga ggcgataacg gcaagtccaa cttcacgtgt
     2281 tgcaaatata gatgcctccc tcggtgtact tccccgttca atatgcttgg taatgttttc
     2341 aagcaccaca atggcgtcgt ccactaccat cccaattgca atagacatgg ctgatagtga
     2401 gattatgttg atggagttgc cggtggcgta aaggtatatg aaaccgctta tcagcgaaac
     2461 ggggagtgtg gtcagaataa taaaggttga gcgccactgc ccaagcgaca ggattacaac
     2521 gagaactaca aatataaagg cataaattag ggtttcggaa aggttgttaa tagagccctg
     2581 gatgaattcg gatgagtcca tgattttaac tatctggacg tcagatggca gttccgtttt
     2641 aatctcctcc atgcggctgc gaaccatctt ggcaattttt accgtgttgg cgccgctttg
     2701 cttctgaacc attagcctga cacccttctg gccgtctatt gtttcgtcaa ccagaatttt
     2761 ctttagggta tccttaacag tggccacatc tttaaggtaa acaacctttc cggcattgtt
     2821 ggagataacc aggtccttta ccacatcgct gctgctgaaa tcgccattgg tcctaacagg
     2881 aaactccatt tttccaactt caatacgtcc ggcaggaagc atcatgtttt cggctccaat
     2941 ggctgctcca acctgttcga ggctgaggtt gtaggagtcg agcttacggg gatcaaccgc
     3001 aacattgata attcttatgg gcttacccat aatggttact gttcccaccc cgtcaattcg
     3061 gcttagccgt tcaactattc tttcgtcaat tattttctcc agcgctgcgt agctcttgtc
     3121 ggctttaatc ccgtagatga gcaccggcac catggacgag ttgaatttca gaatcatggg
     3181 tttttcacaa tcgtcaggaa gcgcattttc ggcaaggctg acagcatccc taacgttgtt
     3241 gctggcttca tccatattta tgccatactc gaactcacag gcaacatacg acacgttgtc
     3301 cttgctcgac gaggttagct tcttgagatt ctggagagag ttaagctggt tctccatggg
     3361 tttggtaacg ttgatctcga tatcttcggc gttagcgcca gggtaggtgg tgataacgct
     3421 tatcatgggg atgtccatat cgggcatcaa gtcaacggga atgtaggtga tagagaatat
     3481 tcccagcact ataatggcaa taaaaatcat tatagtacta accggtttat taactgctgt
     3541 gctatataaa ctcataatgc gtgttttaat tagctgataa aaaaaggttg gtactaaatt
     3601 tttgttaccg cttgtccatc catcaggttg gcctgtccaa ctactatgag ctcatcgcct
     3661 gggttaagcc cgctggtaat ctcaatttta tcgtcgaaaa cttcacccac ttcaataact
     3721 ttccgaacag cttttccatc tgagtaggtg aatacgtagc gctcgttggt tcccgattgt
     3781 ttctgtataa catatccggg aattagtata gagttgccgc ttccaatggt gatgaatgcc
     3841 gtggcaaaca tacccggacg gagcatgttc gaccggttgg gtacaaccac ctcaatggta
     3901 aaagttttgg tgaggttatc aatggtgggg tatatctttt caattttacc gttgaacttt
     3961 tcgccaggga aaacctcggt ggtaatcagt accgaggagc ttagctctat cttgcttagg
     4021 tagcttgacg aaacgccaac cagcagctta agcctgtcga gctgcatgat ggaaacaata
     4081 gctgcctttc ctatgccggc taccggagtc atggcaaaca tttccccgtc gttgatgtac
     4141 tttccggtga taacgccgtc tatgggcgat ttcagggtgg tgttatcgtc aagaaattgc
     4201 aggttggttt tggcgatgtc gtactgcatc ttaacctgat cgtactgctg ttgaggaact
     4261 gctccaacct tcagaaggga gtccatgcgg gccatatcaa tgctaagctt gtcgaactgg
     4321 attttagcct gctggtagct tgtcctatcg agaatggcca ctacctgtcc ggctcttacc
     4381 ctatcaccta cgtcaaccag aactttttcc acccttccgg cggtggcggg tgccagatga
     4441 ttttccttat aggcttgaac ggttgccggg agtcgcagct ggtgttctac cttctgaatt
     4501 tcaacaggtt gtgttgatac tcttacctcg gcctgttttg tttcacccgt aacctcggtt
     4561 ttgtttccgg tgcacgaggc tataagcgct gaaaatgaaa gtattagtaa tgttcttgct
     4621 gttttcatag tctgaagttt attttttttg ttgattagtt agctggtgtt ggtaaagcgg
     4681 attgtcctgt aattttttcg tactcgtttc gggcatttag atactcgaat tgagcctgta
     4741 taaggttgag cctagcctgc atcatggcat tatcggcgtc gttgagttca agcagggttg
     4801 cctgtccggt gttgtaacgg gtttgagcta tagcgtaacc cttttgggca agggtaacat
     4861 tcccctgagc cgattctact ttttttatgg cagtgttaat tgcattaacg gagttttgaa
     4921 cctgggcgga aaggttgctg cgggtaaact ccatctgcat gtcgagctgc tgggcgctaa
     4981 ttttaacctg atttagctgc ttgcgaacgg caaacccgct aaaaatgggg atattaagct
     5041 gaagtccaac aagcgatgtg ggtacccagc gataatcgtt aaacttaaaa tcgttggcct
     5101 gggcctggta ctggtagttc cccatagcca ccagcgaggg caggctgctg cttcttacca
     5161 ttttttcctg aatgccaatc attttacgtg aaagctccag ctgcctaagt gtggtgttgt
     5221 cctgctgcca aacttccgtt ggggaagcaa tttcctcaag ggtaaccgtg cttagctcgc
     5281 tatggtcgag ggttacaggg atggtgtcgt taacgttgag gagcacctta aaaatgttaa
     5341 gcaggttgtt ataggtgttt tttgtctgca taagggtggg atataggttg tcaacctgaa
     5401 ctttgatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc gaaaaacctc
     5521 ccgagtagct gttgtcatag ccaattttca tggctccgcc gcttccaggg ccaaacgtac
     5581 cctcaggaat aaaaattaca ggtagttcaa ggtacctgct gtagctgccg gtagctttta
     5641 tgttggggta aagggcggca tagtactcgc caactttttc ctcctttttc tttagctcca
     5701 gctccgacac cttttgcatc agattttgct ggatggccat gtttacagca tcctttacgg
     5761 tgagcttaag ggtatcggtt tgcccatacc cggacaatgg agtgagcgat actgccattg
     5821 ccactcctaa tccatataac acgtgaaaga ttgtttttcg cataaggtac atattaggat
     5881 ttttaagtgt tagaccattt tatgttttga gtagtaaaaa tctaccagtt caagcccctt
     5941 gctggttgag ataccccgaa taaaatttat agaaatatgt tcgaaaactt cggcaggaga
     6001 aaacatggaa agtggcgatg tttcaaatcc gccttcaaca tgcagcactt ttatctgttc
     6061 aaaaatcagg atggaggcaa tgtcggggtt tatctccttg cggaacaccc catccttaat
     6121 ccccatgatg atgaactgct tcatgttctc cctcattcgg ttgttgaatt cgtttgtctg
     6181 cttgcagatg ctttgagggt aaagtttttg tatgtcctta ataaacaagg ggcttgtccc
     6241 acgcagccgg ttgatatggt cccacataaa gaggatgtag gtttcaatga tgtgtgaact
     6301 tccctctata attcgcttgg cttgttcctt tcggctttca atttcctgca tcacgcaggc
     6361 ttcaaccagc tcgtccttgt tcgagaaatt ctcgtaaagc gtacgcttcg atattcctgt
     6421 ttgctcggca atcatatcca tggttacact tcttatgccc atttgccgga atagaagcat
     6481 agcctggttt actatttgtt cctttactat catttttcgg ttttaccctc ctttaatttg
     6541 accgcaatgt tattacaaaa aactttttaa acaataaaag tttttttaat atttgttaaa
     6601 tattctttaa ttttttgtag cagctcctct atgctttatg cggaattaca cacacttcct
     6661 ttaaatataa agtatttgtt cgaacgggtg gaaggcaaag ctacatcaac aagccgatag
     6721 gcgagaaaag ttgtaagggt gttagttctc ttgtaagcta cacaagggcg aataactact
     6781 tcttaaagtg ctgctagaat cttgctatca tgcaccgaaa atgtgtagaa tgccattata
     6841 gcttagaagg taaatatgag gctaccgcca cgacctaacc agaaaaagca ggatgaagcc
     6901 gaaaatttcg agccttccca gcagcatgtc aagtgaaaga accacaaggc ctggttcggg
     6961 gatggtagaa aagttggaca ttgatccaac cagtccaaaa cctgggccgg cattacccat
     7021 ggtggttacc gaagcagaaa accctgtaag caggtcaacc cccattgcag ccagcagcat
     7081 ggtgctcgcc agcaaaatca ggatgtagaa aacaataaac agggttactg agccaaccac
     7141 ctcgtcagaa atatttctgt tgcctaccct aacggggata acagcctggg ggtgctctat
     7201 ttttagcact tggcttttga gcgctttaaa gaaaataaca accctgtcaa cctttaaacc
     7261 tccggctgtt gaacctgcgc aggcgcattg aatggtaaaa aatatgatga tgacaataga
     7321 taggggtggc caaatagatg aatcggcatt ggcaaaaccc gtggtggtgg agagggaaat
     7381 tacctgaaaa gaggcaaacc taaagcattc gccccatgta tcgtaggttg atccatgaag
     7441 gttgatggca actgcaacta ttccggctat ggttacccaa aagtagtatt taacaatggg
     7501 ggagcggaaa acattgcttt tcttaaaggt aaaagttgag tatagaagcc caaaatgaat
     7561 acccgaaaca aacatgaaga acatggttac catctcaata ggcacgctgt taaaccatgc
     7621 aatgctggtg ttttttgttg aaaacccccc tgtggcaatg gtagcaaagg catgggttac
     7681 cgcatcaaac aggttcatgc caaaaattct taacaggatg atatcgcaaa gggttagccc
     7741 gatgtacacg tagaggatga cgtttagcat cttgcgtgtt cggaatctga agttttcctg
     7801 ggcaagtggt gagatttcca ttctggatag ggtcagctgg gctttaccca gcgaggggag
     7861 aataaccagc atgaaaacaa caatgccaat acctccaatc cagtgcatgg aggagcgcca
     7921 gaatagcagg cttttgggca gggcttccac atcttttaaa actgaagctc cggttgtggt
     7981 gtagcccgaa acgctctcga agaatgcctt caccaccgta aattcaccac cgtaaagtag
     8041 aagcggaagc attccaatga aacatgatat aatccagctg agcacaacaa ccaggtagcc
     8101 ttccttgttg gaaaggctct gtgtctttgg aacaaatatc atggggaaaa ccgcccatag
     8161 ggtggttagc agcagggtga agagtagcgg gtaaaaacca ctatcgtggt aatggatgga
     8221 tagtagaagt gaaaacagga tgaaagctgc gtttagcagg agaacaaatc caatgtagcg
     8281 ggaaagaaca tgaagtctca tctattacct gggtgtattt tttagggtgg taagattctt
     8341 gacagcttcc ttcagctcgt ttacctcatc cttggtatca acagcaacct caaatggacg
     8401 tttatacttt atgaaatcat taatcccctt cgttatcccg attactggct taacaaagta
     8461 gtaggaaatc atgtagtaaa atagcatgga gaacactacg ctggtgataa tcactatcaa
     8521 tccaggcatg attgtccggt aagggctatt tttaagaatg gcagctgttt cagttagggc
     8581 ttgctggttt attagcagga ggttgtccac actgttctgt atggtttcca tcattggtct
     8641 tatagttgta atgtattcgt tcagtgaggc gttagacgat gcaataaatg attctgtagc
     8701 ctgcctgtag ctgttaaaga ctagcgaaat agagtcggta agcgtttttt catcgggttg
     8761 ggttaggttg gttgcagcct tttcaaaatt ttccttaaat tccttcacag aaatgccaaa
     8821 aaggtctgaa gcgctaccct tatcgcctgc aacggcaagc agaatggcac tattttgttt
     8881 ttccaggcta tgcgacattt gccgggcata gtcaatgctt aagtagttat cccttaaaag
     8941 tttatttacc gagttgccta gcttggttag ctcataaatc gaaattagcc ctgaaataat
     9001 cagcagcgaa gccaggatta taaaccccga aagtattttt aggcggatac tcattttttt
     9061 ttacaaatgt ataacgtaat ggtggagttt aatacgattg ggattgttaa attcgatttt
     9121 ttagtaaaag tagattgcac cgcaagaaat ggcaggttga gttgtcaaaa atacattaca
     9181 ttcgtagctg atggacgaca aggatctggt aggcagggtt attgagagta acgaccacaa
     9241 ggcttttgcc cagctggtgg agcggtacca aaaaatggtg gttacaacct gccgggggtt
     9301 tgttcaatcc tacgccgatg ccgaggattt agcacaggat gttttcatcg agctctttga
     9361 gtccctaccc ggctttcgta acgagtcgaa actttcaacc tggatatacc gaatagcggt
     9421 taataaatcg ttaaattttg ttcgccggcg taaacgcgag gtgctgctga acaacctgta
     9481 tttttggaat aaggatagca actcgttggc tcttggacaa gccgtttccc cggagggaga
     9541 gcaggcaatg ctggatgctg aaagaagcaa gcagctgaag ctggccatta acaagctggc
     9601 cgaaaatcaa cgggtagccc taattctaag caggtatcag gaattgtcgt ataaggaaat
     9661 tgccgaagtt atgggagttt ctgtatcttc ggttgaatcg ttactgttca gggctaaaga
     9721 gaaccttcga aaaatgttgc tcaaggggaa aaatatttga aaatcatcgc aagaaatcat
     9781 actacaactt gtctaattat aaaattatga aaatgaactg taagctggca cagcaaaata
     9841 tgctgaacta ccttgatggc acaattgaca agagcattat caacgccttc gaggagcatg
     9901 ttggttcatg cagcagctgc cgggatgagc tacagctact aaaaccaata tacaagcaag
     9961 tggaaggaga tttgggcgag tatgctccaa atccttactt tgccgcaaaa gttttggcaa
    10021 agctgactga aaagcgggag aaaccaacga ccatttttac acaaaagagc tacatactgg
    10081 cagtatcgct agccgctgct gctgtggctg ttggactatt tgtcgggata caatttgtta
    10141 gctcagggtc atcgttggca cagcctgaga ttgctacaat tcttgccaac gattatagta
    10201 atttctcgga tagcaatccg tacaattttg aggttgaaga ggttgatacc tcactaaata
    10261 agtaagccac catgaacttt ttaacgaaaa acagaatact ggtaattgtc gtagtcattc
    10321 tttcactgct gctcctttca attctgggag gtttggggta tggctactac aggagcaaac
    10381 aagcctcaaa gctacctccg cccgccaaca ggtttgagca tcaggcagca tttatttccc
    10441 gatcgctaaa tttttcaccc atacaaaacg agcagttcat caggcttggg gaaggatttc
    10501 atttcaagtt tgactcgctg gcatcacaga ttcggctggt ttcgggtgaa attaataacg
    10561 aaattacccg ggccgaagtt gataccctta agctgaatat gctcattgac aggtatgcca
    10621 gcctgcaacg acagcttaag gagctatcgg taagacatct aatggaagta agggcacttt
    10681 gcaaccctgg tcaacaacaa cagttctgcc ggatggcacg tcaaatcaac cggcgtcacc
    10741 aacaaatgaa ccgaaataac agtaatgatc gcatgcgtgg tatgcccccc aggggccacc
    10801 aaaatcagaa taatttctaa attttaactt aaacttgcaa aactatgaaa cgattgagta
    10861 ttttactgct tggtgccatt atggcagccg gatttacagc caatgcgcag aattgcggta
    10921 agggtatggg catgggaatg ggagcaccca aaatgaatcc tgatagccta caaagctgca
    10981 acatccctaa ccttactccc gatcaacaaa aaaagattta cgatcttcgg gtagcccaca
    11041 tcaaagccgt taccccactt cgtaatcagc tggctgagaa gtatgcccat ctgaaaacac
    11101 tggaatcggc cgaaaagcca gatttgaatg ccatcaacaa aactattgat gaaattgtta
    11161 gcattaaagg gcaacttatg aagagtcgtg ctgctcatag ggccgatgtt tcagcgttac
    11221 ttaccgatga gcaaaaagtt ttctttaacg cccatcaggg gatgggtaat agaatgcatc
    11281 aaaagggcat gggtgctggg atgcatcagg gaatgggaaa gaataaaccc atgggttcat
    11341 gccaacaaat gaactaagcc aattaaaaat aagaattgaa ggtaaaatgc tggcttctcc
    11401 caagcgggga ggccagtttt tttgtatgat gaaaacccta gctgtttcgg gcagttggtt
    11461 attggccatt atcatgaaaa attcaatgtt aaattctcca aaaatttagt aattttgcaa
    11521 agttttttaa ggttttatga tgattggata cggcggacta ccagaggcat ttacaaacta
    11581 cagtacatca aaggtagccg ttctacctgt ggcttacgat ggcaccagca catgggtaaa
    11641 gggcgccgac aagggaccct atgcacttct cgaagcttcc gcaaacatgg aggtttacga
    11701 cattgaaacc aactccgagg tatataggat aggcattttt acagatgaaa tcatggaggg
    11761 cgatttcagc agccccgatc aaatggttag tgcggttcgc agccgaacac taaaattaat
    11821 caacgacggt aagcttgttg caaccatagg gggtgagcat tctgttagca ttggtgctgt
    11881 tcaggctcat gcccgcacct atgaccacct caccgttctt caaattgatg cacatgccga
    11941 tatgcgcagc agctatgagg gtagcccgta taaccatgca tgtgtaatgg ccagggttaa
    12001 ggagctgtgt cctgtggttc aggttggcat tcgtagcatg gatagctccg aaaaaaccaa
    12061 catagatcct catcgtgttt tctgggctca tcagcttgtc gccaacaata gctggatgga
    12121 tgaagccatc gatcttataa ccccaaatgt ttacattaca tttgatttgg atggatttga
    12181 tccttccatt tttcctagca ccggaacacc agaaccggga ggtttgctct ggtaccccac
    12241 tttgacattt ttaagaaaag tgattgaatc aagaaacctt gtaggattcg atattgttga
    12301 gctttgtcca aacccccttg ataaatcttc agactttctt gcagccaagt tgctatataa
    12361 aatactgtcc tacaagtact tgaatacagt cgtctgaatt ttagaaagta taaccaacct
    12421 gaattagata tgaataagtg caagtgtgtt aaagttccac ctcctgaggt cgggggcaag
    12481 gaattcaagc tgaacgctta ctatgaattt gactacatcc ccccagttaa ggataatcca
    12541 tcgttttata aagtttttag ccttgaggat aacgttagtg gcagttttag cattaaggca
    12601 tttaacgagc actttaaaaa gtactgaaag agccagtaaa tccccaaagc aatgcttggg
    12661 gtaggggttt ggtgctagtg ctaaaactag ccagagtcgg cctttagtta ttcaggtagt
    12721 ctatttcgtt gcagaatact agacatctaa atggcttgtc catttttaac agggctggcc
    12781 gggtgtaaat agttgaattt caagtcttcc aaaagttagc tggcaatttc tatcagcttg
    12841 ttcctatccc taataaccac ctcgcgtcgt tttatgctta taataccttc gacctccaaa
    12901 tcggagaaaa cacgggcaag ggctggtcgg gttaccccaa aaaattctgc cagctcctgt
    12961 tgtgatttgg ggagtatcag ggtggcgtta gccccaccgt aggaaagatt tagcaagtaa
    13021 tgtgcaattt tttctttaat ggttttgaag gacaggaacc aaagcctatt ggagagaaat
    13081 tgagttctgt ttgatatgga attcaagtag tttataagga ctgtggcgtt gcgctgcatg
    13141 agcgctagga aatcggcttt aggaatgtag agtatgcggc aatcgtcgag cgcaaccaca
    13201 tcaacaggat actggttatg ttcgccaaaa ataaaggcgt gtgcaatggg ctttggtgcc
    13261 tgcatctcct caattttcag tattttaccc gaaaaatcgg ccatttcacc cttgacactt
    13321 ccctcaacca atatggtaag gcttttcacc tcatcgttcc gctgggctac cagctgccct
    13381 ttactaaaat ttttcaccac atgtggggtt tggcttatca tgttttcaat ctctacgggg
    13441 ctaaccccca tgaaaagtgg tgattttgac aaaatcgggt acatatacga gtaggtttag
    13501 taaatttttg catgaatata gtggtaatta cgtaaattaa cttcaaaaaa acattcctcg
    13561 tagttggggc gaaattcgtc gctcgggtta aatggtctat agaagatctt atgtttgtta
    13621 tatttttgaa ggaatgccct ataattgtaa taattgctat gccatgaacg accaccaaac
    13681 tggcaagcta gacatttcgt ttaataccga cctgccggaa ccattagttt tcagcacatg
    13741 gaagcaccac ttgaactata tccacgatgc cattttaagc attgaggtag gcagcgaaac
    13801 aacttctctc atcaacgaag ttgcagcagg cgttggaggt acactggttg atttatacat
    13861 cggagagtta accccaatgg ttattgccga agaggttatc cagcagcttg taagctttga
    13921 cgcattgggc tatgatagct tttcccgatg gcttggtgtt tccaagagcg ggtaccagaa
    13981 catgtacctg agcgacgggt cagcatggat tttaagattg ggtatggtta accggcgtta
    14041 cgttcatatt catcctgcca gatactcgtt gaataccgtg cggtgccggt cggccaacct
    14101 gaaatttgct attgcatacc ggttgttata cggtatggac gattcaaatt tttcaatcga
    14161 caaggtaaat aggactagag aaaccttggg attaccacct gcagctgaaa aaattgccct
    14221 ctcgggggca ttaagaatcc ttaagcagct aagtgttaaa attgattaaa attaaaccag
    14281 ctcaataata taaccttcta tatgtcagat aaatcaacca ttccaaaaca aatgttttat
    14341 atcatatttt tggtctgaaa tttgtgaaac atcattaaca attttacgtt attagtattg
    14401 gttttaggta caagttttag ggttaatagt tggttaaggt ttaggttagt tttaggttta
    14461 ggaacggagc tgcttttaag ccgctccgtt cttattttaa tagcttaata tctattgcgg
    14521 cagctctcat aatatctttg gtatttaaac gggtaaaggt aacattgtcc tcctaaattt
    14581 acgattctgc gggcacaagc ttactttgtt gcatggatta agcgtaaaat ttccttattc
    14641 aacatttaaa agtttccggg ttgatttctc ttcgacttcc tgtttgtagg gtttctaaaa
    14701 attatgtagg tttgaatttg ctaaacatac ctgttgatga ggcgcaactc cttcctatac
    14761 gcgttaactt tttctgttat ttgctatgcg tgcggtagca aggaacgtaa cgtcacctcc
    14821 gagcaacagg tttttaggta taacgagagt aaagggatat ccactctcga tcctgcattt
    14881 gccagaaatc ttgcgctaat ttggccggta acccagctgt tcaacgggct tgtgcagctt
    14941 tctgactcgc tagctgttga accctgcgtt gcaaagcaat ggcatgtttc agatgatggg
    15001 ttgcagtaca cctttttact cagggacgat gtttactttc atgattctga ggtttttcca
    15061 aatggtttgg gaaggagggt aaccgcttct gactttgttt actcctttag tcggttactt
    15121 aaccctaaac ttgcttctcc cggatcgtgg gtgctatcga tgctcgaccg aaactttgcg
    15181 ggatttacca atgggtgtaa ggctttaaat gataccatac tgataatcag gctttccaaa
    15241 ccttttcctg catttctggg cttggtgagc atgccctact gctttgttgt tcctcatgag
    15301 gccattgatc attatggtgg tgaatttggc cgaaggcctg ttggaacagg ccctttctac
    15361 atgaagtact ggcgcgatgg tgaaaaattg gtgcttcgtc gtaatgccca ctactttgag
    15421 cttgacaatg aagggaaacg actaccttac ctggaatcgg tagtggtaac ttttgtggtg
    15481 gataaacagt cagaatttat agagtttatt agcggaaaca tcgatatgct ttccggcgtt
    15541 catgcctcct caaaggacga gctgctaacc cgaaccggca accttaatcc gaagtatagg
    15601 gaaaaggtac taatgctcac cggcccctat ctgaatacag agtatctggg agtgctcctc
    15661 gactccattg ggctggagag cctagaaaat agtgcgctgc acgatttacg ggttagaaaa
    15721 gctttaggtt atggctttga caggaatgcc atgatgcttt acctccgggg aaatttagga
    15781 tttccggcaa ccagggggat tgtacctcct gctatgcccg gttttgaaac ttcaacagag
    15841 ggtttttact acaaccccga taaatcaagg cagctactag ccgaagctgg ctatcccaac
    15901 ggaaatgggc tcccaccaat taccatttca acaacagacg attaccttga cgtgtgtgag
    15961 tttatccagc accagcttgg cgaggttggc tttgatatta ggattgatgt gctcccgggg
    16021 gctgcctacc gtgacatgat ggctaattcc aaactatccc tgtttagagc ctcttgggtt
    16081 gccgattatg ccgaccccga aaactacctg gccctttttg tgagtagcaa tttttcaccc
    16141 cttggaccta attacaccca tttttcttca tcggaatacg atcaacttta tcgtaaagca
    16201 ttagctgaac ccttgtatga taatcgcttg ccatactacc gggcaatgga ttccttagta
    16261 attgccaatg ctgctgttat acccctgtac tacgataagg tggtacgatt tatttctaag
    16321 gatgttgagg gtatcacctt aaatcctatg aatttgcttg agctaaaacg ggtcaggaag
    16381 tcatactcgg gtcttgcccc tagctgattt tttatggtat taattctaat tcacagataa
    16441 aatgattgtt gttgccgata gcggggccac aaaaaccgac tggcgaatta ttatggaaga
    16501 tttttcttca gcatcgtttg aaaccgttgg ccttagtccc tactttaaca ccgagctgga
    16561 ttttaacgac gccttgacgc atggatttcc taaagaattg aatcctaatg aggtttctaa
    16621 agtttttttc tacggtgccg ggtgcggaac ccaggagcgc ggcagcagag ttgagagcta
    16681 ccttcactct ttttttcctg cagcagaaat atttgcttgg tccgatgcgc tgggtgccgc
    16741 ccatgcgcta cttcacaacg atcagggtat catagtaata ctggggactg ggtcaaacat
    16801 ctcgtactac gacggagcta agctggaggc taaaacgcca tcgcttggct tcatcctggg
    16861 tgatgagggt agcggagcac acatgggaag gctgctgctc agggattttt tttatggccg
    16921 catggacagt gttttgggaa aaaggttgtc ttctaattat gacatggatt tagcacatgt
    16981 tttaaatatg atttacgata gccctaagcc ttcggcctat ttagcttcgt ttgttccttt
    17041 tctgctcaat aatgtagaaa acccatacat atcggattta ataacccgat cgtttgagga
    17101 gatgtaccag ctgcacctga gctgctatcc caatatacga agcattaaaa ttggtgtaac
    17161 cggatcagta gggtgtttgt ttagcagcat tctcgatagg gtggctagtc agcatgggtt
    17221 taaggtttac aaatacgttc gctacccaat cagctctctt gttgattacc acgtcaacct
    17281 ataccattcg gggaggtaaa ccctgagcat ttccagagtg tttcagcgta ttccctgcat
    17341 aacatcgcct gtttttcctc tctcatttcc gaaagcactc gggctaagcc attcatcagc
    17401 ttcatttcaa caagtttgta gcctgcaatc ccgctcagct caatggcatt cctgatgcac
    17461 tgatgggcca tagtaaggtt ttgggtatga aagtagctgg cagccctacc aacttcgcct
    17521 gcaattactt gcgggaagct tcgaataggg ttgagctgtt ggtatatttg ctgaatttgg
    17581 cagtcttctt cggctgagaa gtggttttta ccgctgagta tttcaaagag ttttctgttg
    17641 aagtagttaa caacctggta atgcgatcct gcaatggagc tgatgtcttc aagatagttc
    17701 tcaaccgccg ggtgttgccc taggagaagc agggttggaa caataagttc cgaaaattct
    17761 gcccgctcgg cataagaggt aaattttctg tagtatgaag tggcgcgttc aactatcggg
    17821 gtggtattat tgccattgcc catgattatt tcggcaaaga gctggtttga aaaccacgct
    17881 cctgccacta gcggtgaaac atctgcgggt ggcttaacat tacgtgttgc ctcgtggtta
    17941 ggttgtgcta gcattccgtt tagggttatg aacccattga atgcaagaag agtttgggca
    18001 aaaaagagtt cggaaatgtc ggtagtgttg gacaaatagt gcttaagcac agtggtaaaa
    18061 taaccgttaa ggtggtttag gtcaatgttt tgctccacca ggagctgtcg ccctaatcgt
    18121 gttttggtat attcgggcat gaggacgttc aataggtagc tgctattcct gagcgtagcg
    18181 cagatctccc ttaatagctg gccatgggtg taggcgtttg gacaaagttc aaaaaatttt
    18241 acgagaactt tttcgtttcg gtctctgctg ccggtgtagt agagagttcc ctgcacgaga
    18301 gggtagagct cggtacctgc aaatttttct tctacctgcc gaaaggtttc ttcagtgatg
    18361 cctccggttg agttaacaat atcctggaca ataagcagcc ctgttagttg agggcttgtg
    18421 atgcgaacgt acttaaaata ccggccggtt gattcaacaa cctgttcttc ggtgataatg
    18481 ccgtaggaca ggagttcatg gtatgctgta aagtagttac ccgaaagttt aagatgtata
    18541 gggtagcttc gcttaatgtc ttccttcaaa acacgatggc cattgcccag cctgattatt
    18601 tccgttagga tatcaacttt ttcgtcgggc agtgtagaaa agtaaatttt actcttaaga
    18661 aactctaaga gtatttcgtg ctggttattg agcaaattct gcttctcagg ggtgtaaacc
    18721 tgaataaaca gctgaagaaa gtaaggatag ctaagcgttt gccttaaatc gggat
//
LOCUS       DAOPHX010000040        18767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_3233_length_18767_cov_6.189664, whole genome shotgun sequence.
ACCESSION   DAOPHX010000040 DAOPHX010000000
VERSION     DAOPHX010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 18767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18767
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3233_length_18767_cov_6.189664"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..1328)
                     /locus_tag="PK502_04595"
     CDS             complement(291..1328)
                     /locus_tag="PK502_04595"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPH00129.1"
                     /translation="MRKLLIILPILLACAASTKVKAQNIPATRHRVMFYNVENLFDPF
                     DDSLTLDEEFTPFGDRHWTWKKMEQKLNGIYKVILAVGEYDPPVLVGLCEVENGFVLH
                     QLVSQTPLSKFEYRVVHRESPDRRGIDVALLYRPDRFTLQEKEFVTVRFPDEPWRTTR
                     EILYVRGLLGGDTLHVFVNHWPSKYGGELESEGGRYAAAYALKNKVDSIKIFYPDARI
                     LIMGDMNDEPESDPVARGFGACLTAETACPYDFVNLSAILKQRGQYSYKYQGVWGIID
                     QLIASRSMLDSRHVLYTFTGNAGVFSAPFLLEDDERYAGKRPFRTFIGFKYHGGYSDH
                     LPVYLDLFSNK"
     gene            complement(1330..2775)
                     /locus_tag="PK502_04600"
     CDS             complement(1330..2775)
                     /locus_tag="PK502_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00130.1"
                     /translation="MAKLPTACLLAVASIFALCHSACVRDDFSESPALKLSFSADTVL
                     FDTVFTTIGSSTQYFKVYNRSGHDLHISSIRLAGGSTSPYRINVDGRSGVTFSNVSLR
                     SRDSLFVFVEVTVDPTSLNSPLLVADSIMFSTNGNIQDVKLVAWGQDVHLLRSEIISN
                     DTVLATDKPYLIYSYLLVDTATTLTIPAGVQMYFHNQAMLVVAGTLKVEGEQGSPVVM
                     EGDRLEDFYRDKAGQWGGIWLTAGSRYSAISWAEIRNAITGITVDTCFTSHAPTLTLS
                     HTRIENCSYVGLLARGAKVKADNCLVSNAAQVCVALTMGGEYRFYHCTVANYWGDYIY
                     RKGPALLLNNYYLYRLEENGPLYVEPRDLVEASFFNSIVYGSMSSEFQVDNIVNGAPV
                     AAQMNYHFEDCILKVPQNFDLTDTEKFTRVFTLDPKFKDSNAHVFLLDTLSPAMGIAN
                     PAVATDYPIDLNGFNRLTDGKPDLGAYERQE"
     gene            2910..4562
                     /locus_tag="PK502_04605"
     CDS             2910..4562
                     /locus_tag="PK502_04605"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPH00131.1"
                     /translation="MAKNWYVYLLLTVTLMACSKREKADLIVTNAKIYTVDSTFSTHS
                     SMAVVNGIVKELGDSVYIMSKYTAKKVVDAGGMYVYPGFIDSHCHFLGYGLSLNKAML
                     SEASSWDEVVARLVEHQKRFPTSWVEGRGWNQNEWKVREFPTNELLNRAFPDKPVYIV
                     RIDGHAAIANNAALKIAGVTASTRVEGGEVVLQKGKPTGLLLDNAMELVRRIIPKPTI
                     DEKAAALQNAQKKCFAVGLTGVQDAGLDVDEVLLIDSLQQSGKMEIWVNAMLNPTPAN
                     YERFISKGVYRTNKLTVRSIKLYADGALGSRGALLLEPYSDDRGNRGLQVEPTEKLDS
                     LCGMAFKAGYQVCTHCIGDAAVRLMLDIYSKYLPAGNILRWRIEHAQVVDRNDLPRFG
                     SLAVIPSVQTTHATSDMDWADERLGSRIKNAYAYQELLLQNGWIANGSDFPIESINPL
                     YGFYAGVARKNLEGKPDGGFQPENALTRKQALCAMTIWAAKAAFCEDARGSLEPGKAA
                     DFVILNTDLMNDELPAIPRATILKTFVSGKEVFGSNAFSHGN"
     gene            4552..5019
                     /locus_tag="PK502_04610"
     CDS             4552..5019
                     /locus_tag="PK502_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPH00132.1"
                     /translation="MEIRQAQKQQLIEVLYIIRECSNQLVEKGVNFWSNSHADMEEIS
                     SDIEQGYVYVLVNNHVSIATVTLKPHATNQKVMTISRLAVFPAFQRKGLASMLISFGI
                     DLAQKGNFTAMEGYSPLYDSNLAALLEKRGFERKGTLSPPSEKIGSIIFEKLL"
     gene            5452..6714
                     /locus_tag="PK502_04615"
     CDS             5452..6714
                     /locus_tag="PK502_04615"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPH00133.1"
                     /translation="MITIDSYSFKGKKAIVRVDFNVPLDKKTLEVTDDTRIRGAVPTI
                     KKILGDGGAVILMSHLGRPDGTPQDKYSLRHVVPAIEKNLGQKIKFAADCMGEETKKM
                     AAGLKPGEVLLLENLRFYEEEEGKPYSLPETASEEEKKEAKARVKESQKNFVKQLASY
                     ADCYVNDAFGTAHRAHASTALIAAHFPGNSMFGYLIESELKAMDKVLKSPTKPFTAIM
                     GGAKVSDKILIIENLLDRVDNLIIGGGMTYTFICAQGGKVGKSICETDKLELANQLLE
                     KAKAKGVKVYLPVDAVNADAFDANANTQITPIDSTPDGWMGLDIGPKTIAKFREVILE
                     SKTILWNGPMGVFEMDKFAAGTSEIAKAIAEATQRGAFSLVGGGDSVAAVNKNHLADK
                     ISFVSTGGGAMLEYMEGKELPGIKAIRG"
     gene            6824..7807
                     /locus_tag="PK502_04620"
     CDS             6824..7807
                     /locus_tag="PK502_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPH00134.1"
                     /translation="MIPSSSIFSLKPDGDFEKIALEVFRFQSQHVRVYHDYIQQLGVN
                     PDAVASTDEIPFLPIQLFKTQDVIAEACQPEATFTSSGTTGNEVSRHRVADLSLYRES
                     FTRTFSIFYGSPSDYCILALLPSYLERSGSSLVFMVDELIRQTGNPDSGFYLNQLYAL
                     TEKLSALDRSGQKTILIGVSFALLDLAERFTFNLQNTIIMETGGMKGRRKEVVREELH
                     STLCSAFGVNVIHSEYGMTELLSQAYSTGKGMFRCPPWMRVVIGSTDDPFCLVGDGRT
                     GIIKIIDLANLYSCSFIQTQDMGKLHSDGCFEVLGRMDDAPIRGCNLLVYD"
     gene            8087..8785
                     /gene="radC"
                     /locus_tag="PK502_04625"
     CDS             8087..8785
                     /gene="radC"
                     /locus_tag="PK502_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPH00135.1"
                     /translation="MEQEYKRLTIKDWAVEDRPREKMLHQGIGSLSDTELIAILLRSG
                     NPEETAVELAQRILNMAQNNLNELGKFTIHQLTQVKGVGEAKAITLLAALELGRRRKS
                     VEAINREQITSSRNVMEIFQPLLADLPHEEFWVLLLSRANKVLDRVRVSQGGVTGTVT
                     DVKLIIRAAVEKLASAIIVVHNHPSGNPKPSEKDIAITEKLKQAVDFFDIALLDHLII
                     TDSKCYSFADEGTL"
     gene            8850..9677
                     /locus_tag="PK502_04630"
     CDS             8850..9677
                     /locus_tag="PK502_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPH00136.1"
                     /translation="MLNQSVKKFWKKLPRLCLIGMVGIMPSINKVAGQGFESLYEAKV
                     FTHKGKTLPYRIMYPKNFNPEQKYPLVIFLHGSGERGNDNQKQLVHGASLFANDSVRS
                     RFPAIVVFPQCPEDDFWANIKENPNQQGYRSIEFLPESKPTAAMDLLIRLVNGLKRES
                     YVDSRRIYVGGLSMGGMGTFELVARMPKVFAAAFPICGGSNPESAKRYAHKVKFWVFH
                     GAKDDVVPPQYSIDMVSSIRKLGGDARLTVYPDAGHNSWDQAFREPELLLWLFSCKK"
     gene            complement(9715..10398)
                     /gene="clpP"
                     /locus_tag="PK502_04635"
     CDS             complement(9715..10398)
                     /gene="clpP"
                     /locus_tag="PK502_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPH00137.1"
                     /translation="MNEFEKYATKHRGISSLSLQRFGTITNSYISPTIIEERQLNVAT
                     MDVFSRLMMDRIIFLGVPIDDDVANIIQAQLLFLESTDPSKDIQIYINSPGGSVYAGL
                     GIYDTMQYITSDVATICTGMAASMAAVLLTAGEKGKRTALPHSRVMIHQPMGGTEGPA
                     TDIEITTREILKLRDELYEIIATHSGNPIDRIRKDSDRDYWMTAREAMEYGMIDEVLT
                     RSKNLKEAK"
     gene            complement(10525..11886)
                     /locus_tag="PK502_04640"
     CDS             complement(10525..11886)
                     /locus_tag="PK502_04640"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPH00138.1"
                     /translation="MNVVKENTNEQTISIRARVEQADYVEKVEKKLKDYRKNSNIKGF
                     RPGMAPAALIHRMYYKYVLVDEVTHIAFDAVMDYIDKEKIKILGEPLPSEGQNTINWD
                     TDKEFELAWEIGVAPEFEIKLGKKDKIPFYNIAPDDKIRKQYIDSYLMRFGHFAEADE
                     TNERGLIEATLTELNSDETPREGGLFVERATISVELVADPDERAKLVGVKVGDVLTID
                     VQKAFPNEADRASLLSTQKENLPNIQPVFQLTVNKTSNYQPAELNQELFDRVFGEGVV
                     TTEEEFYQKIDEEIESGLKNEGENRFAVELRNILLDKIDMQLPKDFLIRWLTTINEGK
                     FTREQVENEYPLFEGDLKWQLIRNKIASEQNFTVTNEEMESFAMEYAQQQFMAYGMSY
                     LPKEYIERYAKDILKNQDEIRKMQEGIISRKVIAWFKENLPLDVKEITHEEFDKLEPV
                     SGK"
     gene            12093..14396
                     /locus_tag="PK502_04645"
     CDS             12093..14396
                     /locus_tag="PK502_04645"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPH00139.1"
                     /translation="MNSTEFHRNILLVLILLTFSLSYSQAQSDTVRIGEMLQRAEVLR
                     TTNPDSAIGLAIQALNIIDGSDNKELMVRVEHTLGNCYYSRNDFELALSHLKKSLQLA
                     TEEGRKFWVAMNLNRIGNVYQLKSDYGSALQAYMEALNINRSIDNKSQLARTLVNIGT
                     VYSITGSYKEAIENMLKALETYEQAKDYEGIAWASLSISRLFSRIDLYEKALQYAESS
                     LTHYRQLSNINGVTLAKTELANIYYHLGMYPKAKEMATDVLATNQQNQNIYGQAANHL
                     LLGIINFELGDYTSAKSNLLTSLELKRQVNDSIDLAKLSRYLGEVNLKSGNVAAGLES
                     LEQAMRIAQRQRVLTDQRDIFLSLSQAYRLRGDYRRSLDYFTRYSAVKDSINASQIAR
                     MEMQYDFDKREREQELISRQREAIQQAKLERQRMLTLFISIALLLAVALAGVIFYFYR
                     NKKKINALLVAQNQEILRQKEEIEAQKEEIESQRDLAERQRDQIAGQQKQITDSIRYA
                     SRIQSAVMPRTDTLATFLTDYFIYFQPKNIVSGDFFWVTRLNDGRIAIAVADCTGHGV
                     PGAFMSILGITLLKEIIAYQQVNTAGDLLDKLRQMVIAALNQSGQVQDSHDGMDMGLL
                     VVDAKGRKAEYAGAYMPLLVSRNSELPEVSAGVQPETANGWSIYEIKGDKMPVGYHLA
                     GERPFTTHRFDLLSNDTFYLMSDGYTDQFGGALNQKLMMVNFKKNLLGLQTLSLSRQN
                     EELQKQLIEFQGTQKQVDDILVMGFRL"
     gene            14690..15658
                     /locus_tag="PK502_04650"
     CDS             14690..15658
                     /locus_tag="PK502_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPH00140.1"
                     /translation="MSNKKFLVYIAVTVAMAFWALSFIWYKDAYVYYRPITLVFFRLV
                     ISAAFLYVFSLAMRKLERVKKGDLKLFFLLAFFEPLLYFIGESFGMTLISSTMAAVIV
                     STIPLLSPVGEFFMFHRRISTTNLMGIIASMLGVCLVIFHNGFGQVDVNFTGVALMFL
                     AVFSTVGYSLIVGKLTVKYNVYSIITYQNTIGALYFMPLFLIFDFNHFQTVGISWQAM
                     VPVIKLGIFASTFAFMLFTYSIKHLGITRANTFSNAIPVLTAIFAYFLLDEALTPVMI
                     AGILVVVSGLFLSQISPKTKTQIELKVLPEHPVVGIGLRLRKLFRK"
     gene            complement(15920..17230)
                     /gene="murA"
                     /locus_tag="PK502_04655"
     CDS             complement(15920..17230)
                     /gene="murA"
                     /locus_tag="PK502_04655"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPH00141.1"
                     /translation="MATFEVIGGKRLKGDLFPQGAKNEALQVICATLLTPEKITINNI
                     PAIRDVVKLIELLQGMGVEVTRLSESSYSFIARNIDIDYLTTDDFKVKAAAIRGSIMV
                     VGPLLARYGKAYAPKPGGDKIGRRRLDTHLLGFQKLGVEFDFDVNDNFFTLEGKHLKG
                     SYMLLDEASVTGTANILMAATMTPGKTTIYNAACEPYVQQLCHMLVSMGARISGIGSN
                     LLTIEGVDGLGGCEHTILPDMIEIGSFIGLAAMTCSDITIRNVSFGNLGIIPDAFRRM
                     GINFEVTNDDIHIPPHEHYEIDTFIDGSILTLADAPWPGLTPDLLSVFLVVATQAKGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGHDRQLRLRPTVMTSPDIRAGV
                     ALLIAAMSADGKSIIHNIEQIDRGYQNIDGRLNAIGAEIHRIDR"
     gene            complement(17237..17821)
                     /locus_tag="PK502_04660"
     CDS             complement(17237..17821)
                     /locus_tag="PK502_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HPH00142.1"
                     /translation="MDYNTQRKKLALPEYGRHIQQMVDYIASVEDRDERNRLAKSLVA
                     IMGNLNPHLRDINDFKHKLWDHLFIMSDFKLDIDSPYPIPSVEQYNEKPRRVPYPGNP
                     IKFKHYGRIIEMMIEKAVAMEEGPEKDALKQLIANQMKKANITWNKESVSDDDIFRDM
                     QTLSNGMLVMAPGSKLVDNKEFKNKPRTKYHKKH"
     gene            complement(18034..>18767)
                     /locus_tag="PK502_04665"
     CDS             complement(18034..>18767)
                     /locus_tag="PK502_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPH00143.1"
                     /translation="IREFIEARRNEGIDETTTLVDYLENVALLTSLDNDDPNDRNRIS
                     VMTIHSAKGLEFDYVFLTGLEENLFPSNMSMNTPEELEEERRLFYVALTRAAKGVTIS
                     FAQTRLKWGNINISNPSRFISEIDQRYVESPFEPDGGGHLKATSELSRQTSINNFGKN
                     SIGNRVAPYSQPPGRTPVSVPENLKELPPEEICEGVEVYHGKFGQGKVLSVEGEGANK
                     KATVLFTEAGQKNLLIKFANLKGIA"
BASE COUNT         5061 a         4502 c         4428 g         4776 t
ORIGIN      
        1 aacaagttgt tcggttaaat attttacaaa gttaagtagt tgatagatag atagaaaaaa
       61 gtttgcgaaa aaacgaaccg agcttgcgag ctcaacgaag ttaattttta gaacccaccc
      121 taaaatactg cattcaacaa agtgttaatt ttatttgggc tgattccgtt tgcaaggttc
      181 cccactttta ctaactttat tccgggtgat gacaacgctg gaggtaaccc ccaaacaaaa
      241 tcaacgctcg gccgttatgg acaacggagg ctctccacat gaaaaacgcg ctacttatta
      301 ctgaacaaat cgaggtaaac cggtaaatgg tcgctgtagc caccgtggta cttaaaccca
      361 ataaaagttc gaaatggtcg tttgccggca taccgctcgt cgtcctcaag caaaaagggt
      421 gcgctgaaaa ctcccgcatt gccggtaaag gtgtacagca catgccggct atccagcatg
      481 gagcgtgacg caattagctg gtctattatg ccccacactc cctggtactt gtagctgtac
      541 tgtccacgct gtttcagaat tgccgacagg ttaacaaaat cgtaaggaca ggcagtttcg
      601 gctgtgaggc aagcgccaaa tccccttgcc acgggatccg attcgggctc atcgttcatg
      661 tcgcccatga tgaggatacg ggcatcgggg tagaaaattt ttatggagtc aaccttattt
      721 ttaagggcat aggcagcagc atagcgaccc ccctctgact ccagctcccc gccgtacttc
      781 gatggccagt ggttaacgaa aacatgtagc gtgtcgcctc ctagaagccc acggacgtaa
      841 agaatttccc gggttgtgcg ccagggttca tccggaaacc tgaccgttac aaactccttc
      901 tcctgaaggg tgaacctatc gggacgatag agcagcgcta cgtcaattcc ccgccggtcg
      961 ggcgactcgc gatgcaccac ccggtactcg aattttgaaa gcggcgtttg gcttaccagc
     1021 tggtgcagca caaagccatt ctccacctcg caaagaccta ccaaaacagg cggatcgtac
     1081 tcgccaacag ccaaaatcac cttgtaaatc ccgtttagct tctgctccat cttcttccat
     1141 gtccagtgcc tatcgccgaa tggtgtaaac tcttcgtcga gggtaagcga gtcatcgaat
     1201 ggatcgaaga ggttttcgac gttgtagaac atcacgcgat ggcgcgtggc agggatattc
     1261 tgagctttaa cctttgttga ggcagcacaa gcaagcaaaa tgggcagaat tatcagaagc
     1321 ttcctcatac tactcctgtc tttcgtatgc accaaggtcg ggtttaccat cggtaagccg
     1381 gttgaaccca tttaggtcaa tagggtagtc ggtagcaaca gcagggttgg caattcccat
     1441 ggcggggcta agcgtgtcga gcagaaaaac atgggcattt gaatccttga acttggggtc
     1501 gagcgtaaat acccgggtaa atttttcggt atcggtgagg tcgaagtttt ggggaacctt
     1561 caggatgcaa tcttcaaaat ggtagttcat ttgtgcggca accggtgcac cgttaactat
     1621 gttgtcaacc tgaaattcac tgctcatcga cccatacaca atgctgttga aaaatgaagc
     1681 ctccaccaaa tcacgcggtt caacgtaaag cgggccgttc tcctcaagtc ggtaaaggta
     1741 gtagttgttt agcagcagcg caggcccttt gcggtagatg taatcgcccc agtagttggc
     1801 aacagtgcag tggtagaagc ggtattctcc gcccatggta agggccacac aaacttgcgc
     1861 agcgttcgac accagacagt tatcggcctt tacttttgcc ccacgtgcca gcaggcccac
     1921 atagctgcaa ttttcaattc tggtgtggct aagagttaaa gttggcgcat ggctggtaaa
     1981 acaggtatca accgtaatgc ctgttatggc atttctaatc tctgcccagc tgatagcgct
     2041 ataccggctt ccggccgtaa gccaaatccc tccccactgg ccggccttat cgcggtaaaa
     2101 atcctcaagc ctgtcgccct ccattacaac gggactacct tgctcgccct caacttttag
     2161 tgttccggcc acaaccagca tggcctggtt atggaagtac atctgtaccc cggcgggaat
     2221 ggtgagggtg gtagcggtat ccaccaaaag gtagctatag atcaggtagg gcttgtcggt
     2281 agccagcacc gtatcgttgc taatgatctc tgacctgagt aggtggacat cctgacccca
     2341 cgccaccagc ttcacatcct gtatattgcc attggtagaa aacatgatgg agtcggcaac
     2401 aagcaacggc gagttcaggg acgtagggtc aaccgttacc tccacaaaaa cgaaaaggct
     2461 gtcgcgggag cgtagcgaaa cattgctgaa ggttacgccc gaacgaccgt caacgttaat
     2521 ccggtagggc gaagtgctgc ctccggcaag gcgaatagac gaaatatgaa ggtcgtgccc
     2581 cgaccggttg tacaccttaa aatactgcgt tgaagagcct atagtggtaa atacggtgtc
     2641 gaatagaacc gtatcggccg agaagctgag tttcaaggct gggctttccg aaaaatcgtc
     2701 ccgcacacat gccgaatggc ataaggcgaa aatagatgct actgctaaaa ggcaggctgt
     2761 tggtaacttt gccattacta atgtctattt caacattcag agattacaat gtaacaaatt
     2821 tacaaaagct gttgagggta ttaaaacaaa gactaaattt gatagctgtg gctggttaaa
     2881 taatggccta aaaatcaata catacaaaga tggcaaaaaa ttggtatgtt tacctattac
     2941 taacggttac cctcatggca tgtagcaaac gtgaaaaggc cgacttaatt gttaccaacg
     3001 ctaaaattta caccgttgat tcaactttca gcacccatag cagcatggcg gtggttaatg
     3061 gaatagtaaa ggagcttggc gattccgttt acatcatgtc gaagtacact gccaaaaaag
     3121 tagttgatgc cggaggaatg tatgtttacc ctggctttat tgactcgcac tgccattttc
     3181 tgggttacgg gttaagcctg aacaaggcca tgctttcgga ggcaagttcg tgggatgaag
     3241 tggtggccag acttgtagag caccaaaaga gatttccaac aagctgggtt gagggtaggg
     3301 gttggaacca gaacgagtgg aaggttaggg agttccccac caacgagctg cttaaccggg
     3361 cattccccga taaacctgtt tacattgttc ggattgacgg ccatgcggcc attgcaaata
     3421 atgctgcttt gaaaattgcc ggcgttactg ccagcacacg agttgaaggc ggtgaagtgg
     3481 ttctgcaaaa aggaaaacct acggggttgc tgctcgacaa tgctatggag ctggtgcgta
     3541 ggataattcc caagccaact atagatgaaa aagccgctgc gttgcagaat gctcagaaaa
     3601 aatgctttgc cgttgggctg accggagttc aggatgcagg gctcgatgtt gatgaggtgc
     3661 tgcttattga ctcgctgcag caatccggca aaatggaaat ctgggtgaat gccatgctca
     3721 atccaacccc ggcaaactac gaaaggttta tttcaaaagg ggtgtatcgg accaacaagc
     3781 ttaccgtccg gtcaataaag ctatacgccg acggggcgtt gggctcaaga ggcgccctgc
     3841 ttcttgaacc atactctgac gatcgtggta accgtggcct tcaggttgaa ccaacggaaa
     3901 aactcgattc gctctgcggc atggcattta aagccggata ccaggtgtgc acccactgca
     3961 ttggcgatgc tgctgttagg ctcatgcttg acatctactc aaaatatttg cctgcaggaa
     4021 atatcctgag gtggcggatt gaacatgcac aggttgttga caggaacgat ttgccacgtt
     4081 ttggttcact tgccgtaatc ccctcggttc aaaccaccca tgccacctcg gatatggact
     4141 gggcagatga aaggctgggc agcagaatta agaatgccta tgcctatcag gagctactgc
     4201 ttcaaaacgg ctggattgcc aacggaagcg atttcccaat tgaaagtatc aacccgcttt
     4261 acggtttcta cgctggcgtt gcccgtaaaa acctggaagg caaacccgac ggaggatttc
     4321 aacccgagaa cgcccttacc cgaaaacagg ccctctgcgc catgactata tgggctgcaa
     4381 aggccgcttt ctgcgaagat gctaggggta gccttgagcc aggaaaagct gccgactttg
     4441 ttattctcaa caccgacctg atgaacgatg agctgccggc aattccaagg gcaaccatcc
     4501 tgaaaacctt tgtctccggc aaggaggttt ttggttctaa cgcttttagc catggaaatt
     4561 agacaagcac aaaaacagca gctgattgag gtgctctaca tcattcggga atgctccaac
     4621 cagctggttg aaaaaggggt taacttctgg agcaacagcc atgccgacat ggaggaaata
     4681 tcgagtgata ttgaacaggg ctacgtgtat gtcctggtaa acaaccatgt ttccattgca
     4741 accgtaacct taaaaccgca tgccaccaac caaaaagtga tgacaattag tcgtttggcc
     4801 gttttcccag ctttccagcg taaggggttg gcctccatgt tgatctcatt tggtattgat
     4861 ttagcccaaa agggtaactt tactgcaatg gaaggctact cgccactata cgatagcaat
     4921 ctggcagcac ttttggagaa aaggggattt gaaagaaagg ggacactatc cccaccttcc
     4981 gaaaagattg gaagtataat ttttgaaaag ctgctttgac acatcattag cttgatgaag
     5041 caaaacacag aggaaagtag catattaacc aggaagactt taacatttaa tgcagataaa
     5101 acaaatcccc attggcagct ttaccccaca tgcagctgaa aattttctaa attatttacg
     5161 ccagactacc cgggcttgac ttcaaatcat ttcacgtttg cgattcagcc ccagcatacc
     5221 ccggagacga cgagctgctg agttgattat ttatttttac gacctcatgc tcctgaaaat
     5281 ttacttagaa attcaggaaa tctcactact gctgtaagaa gtagcctttc ctattaaaac
     5341 ataccttaaa atgatatttt tcaaacgatt gcgtaaatat attcgcttga aatagcctat
     5401 tttttgcaac gctatgctaa ctttgctttg aaatcaaata cccgttaagc catgataacc
     5461 attgacagct atagctttaa agggaaaaaa gccattgtaa gggttgactt taacgtcccc
     5521 ctcgacaaga aaacccttga ggtaaccgat gacacccgga taaggggtgc cgttccaaca
     5581 ataaaaaaaa ttttgggtga tggaggagct gtcatcctca tgtcgcacct tggccggccc
     5641 gacggcacac cacaggataa gtactcgttg cgccacgtgg taccggctat tgaaaaaaat
     5701 ctggggcaga agataaaatt tgccgccgac tgcatgggcg aggaaaccaa aaaaatggca
     5761 gctggcctaa aacccggcga ggtgttgctg ctcgaaaatc ttagattcta cgaggaagaa
     5821 gaaggtaaac cttacagcct tcccgaaacc gccagcgagg aggaaaagaa agaagccaag
     5881 gcaagggtta aggagagcca gaagaatttt gttaagcagc tggccagcta tgccgactgc
     5941 tacgttaacg atgcttttgg aactgcccac cgggcacacg cttcaacggc gcttattgca
     6001 gcccactttc ccggcaacag catgtttggc taccttattg aaagcgaact caaggcaatg
     6061 gacaaggtgc tgaagtcgcc caccaagccc tttacagcca tcatgggagg cgccaaggtg
     6121 tccgacaaaa tcctgataat cgaaaacctg ctcgataggg ttgataacct gataattggt
     6181 ggcggtatga cttacacctt catctgcgcc cagggaggaa aagttggtaa gtctatctgc
     6241 gaaaccgata agctcgagct ggctaaccaa ctgctcgaaa aagcaaaggc caaaggagta
     6301 aaggtttacc tgcctgttga cgcagtgaat gccgatgcat ttgatgcaaa tgccaacacc
     6361 cagattaccc ctatcgacag cactcccgac ggctggatgg gtctggatat tggccccaaa
     6421 accatagcta agttccgtga ggttatcctc gaatcaaaaa ccatcctctg gaacggcccc
     6481 atgggcgttt ttgaaatgga caaatttgct gccggaacat cagaaatagc caaggccatt
     6541 gccgaagcaa cccaaagggg agcgtttagc ctggttggcg ggggcgactc cgtagctgct
     6601 gtcaacaaga accacctggc cgataaaatt tccttcgtct ccaccggtgg gggcgccatg
     6661 ctggagtaca tggaaggaaa agaactgccc ggaattaagg caattagagg gtaacaaccc
     6721 tctaatcacg ccatttaagc ataccgtcag ggttttaaaa aaagccctga ctttgtattt
     6781 tcagctactc ataacccgta aaagcaggta aaggcaaaat caaatgattc ccagctccag
     6841 catattcagc cttaaaccgg atggtgattt tgaaaaaata gccctggagg ttttcaggtt
     6901 tcagtcccag catgtaaggg tttaccacga ctacattcaa cagcttggcg taaaccccga
     6961 tgcagttgct tccactgacg aaattccgtt tctccccatt caactcttca aaacgcagga
     7021 tgttatagcc gaagcatgcc aaccggaggc aaccttcacc agcagtggaa ctacgggtaa
     7081 cgaggttagc cgccatcggg tggccgattt atcgctctac cgtgaaagct tcacccgcac
     7141 gttttcaata ttttacggtt ctccctcgga ctactgcatt cttgcacttc tgccctcata
     7201 ccttgaacgt tcgggctcat cgctggtgtt catggttgat gagcttatca ggcaaaccgg
     7261 caatcctgac agcggtttct acctcaacca gctctatgcc ctaacggaaa aactcagcgc
     7321 ccttgaccgc tccggtcaaa agacaatcct tatcggggtc tcgtttgctc tgctcgacct
     7381 ggccgaaagg tttaccttca accttcagaa taccatcatc atggaaaccg gaggtatgaa
     7441 ggggcgtcgt aaggaggttg tcagggagga gctgcactca acgctttgca gcgcatttgg
     7501 ggtaaacgtc attcactccg agtacggcat gacggaattg ctttcacagg catattctac
     7561 cggtaaaggg atgtttcggt gtccaccctg gatgcgggtg gttataggta gcaccgatga
     7621 ccccttttgc cttgtagggg atgggagaac aggaattatc aaaattattg acctggccaa
     7681 cctttattcc tgctcgttca ttcaaaccca ggatatggga aagctccatt ccgatggttg
     7741 tttcgaggtg cttggtcgta tggatgatgc acccataagg ggttgcaact tactggttta
     7801 cgattgaata gacctggaag cattgccaaa gtgaacctat gtgcacaacc catagcatct
     7861 actttctacg cttgttttaa attttacaca tccccaacat caggccacac cattgcatgt
     7921 agcatggcat gcaacccttt ttccctgtta aaaagttgca ttcacctgta tgaactatgc
     7981 aataaaggag ctaaattagc cttttttgga ggtttgatgt ctaaaactaa ttcttgatac
     8041 agcagctgtt tcttgccgtt catacccagt tgaaaaaggc aaaccaatgg agcaggagta
     8101 taaaaggcta accataaagg attgggctgt ggaggaccgg ccccgggaaa aaatgcttca
     8161 ccagggaatt ggaagcctaa gtgatacgga gctgattgcc atcctgctgc gttccggaaa
     8221 ccctgaagaa acagcagttg agcttgccca acggatatta aatatggccc agaataacct
     8281 caacgagctg ggtaagttta ccatccatca gcttacacag gttaaaggag taggcgaagc
     8341 caaagccata acccttttgg cagcgttgga gttgggccgt aggcgaaagt ctgtggaggc
     8401 cataaaccgc gagcagatta catctagccg gaatgtgatg gaaatttttc agcccttact
     8461 ggccgacctg ccccacgagg agttctgggt gctgctcctg agccgggcaa acaaggtgct
     8521 ggatagggtt agggttagcc agggtggggt aaccggcacg gttaccgatg taaaactaat
     8581 cataagggca gccgttgaaa agttggcctc tgctattatt gtggttcata accacccgtc
     8641 gggaaacccc aaacctagcg aaaaggatat tgccattacg gaaaagctca agcaggctgt
     8701 ggattttttc gatattgcgc tactcgatca ccttatcatt accgatagca aatgctactc
     8761 atttgcagac gagggaactc tgtaatcaac cacttgaata gccataaaac ctgacatgca
     8821 gaaaatacta atagctaaca acttatacca tgttaaacca atcagtaaaa aagttttgga
     8881 aaaaactacc ccggctatgc ctgataggca tggttggaat tatgccttca ataaacaagg
     8941 tcgccgggca aggctttgaa tcgctgtacg aagctaaggt ttttactcac aaggggaaaa
     9001 ccttacccta ccggataatg taccctaaaa atttcaaccc agagcagaag tacccactgg
     9061 tgatctttct gcatggttcg ggtgagcggg gcaacgataa ccagaagcag ctggttcatg
     9121 gagcgtcact gtttgccaac gattcggttc gatcgcgttt cccggcaatt gttgtttttc
     9181 cacagtgtcc cgaggatgat ttttgggcaa acatcaagga gaaccccaac cagcaaggat
     9241 accgttctat tgagttttta cccgaaagca aacccacagc agctatggat ttacttatcc
     9301 ggctagtgaa tggcctcaaa cgggaaagtt acgttgatag caggcggata tacgttggag
     9361 gcctttccat ggggggtatg ggaacctttg agctggtggc aaggatgcca aaagtatttg
     9421 ctgctgcctt ccccatttgc gggggaagca atccggaatc ggcaaaacgc tatgcgcata
     9481 aggtcaaatt ctgggttttt catggtgcaa aggatgatgt agttccaccg cagtactcca
     9541 ttgatatggt tagctccata cgaaagctgg gtggcgacgc aaggttaacc gtatatcccg
     9601 atgctggcca caatagctgg gatcaggcat tccgagagcc cgagctgctt ttgtggctat
     9661 tcagctgcaa aaagtaaaaa agcgccttta aggcgctttt ttgcagtaaa ggaatcattt
     9721 agcctccttt aagttttttg atcgggtaag gacctcatca atcatcccat actccatggc
     9781 ttccctggca gtcatccagt agtcgcggtc ggaatccttc cgtatccggt caatggggtt
     9841 tcccgaatgg gtggcaataa tttcatagag ctcgtcgcga agcttaagaa tttcacgggt
     9901 ggtaatctcg atgtcggtag ctggcccctc agtcccaccc attggctggt gaatcattac
     9961 ccgcgaatgg ggcaaagctg ttcgtttacc cttttcgcca gctgtgagta acaccgcagc
    10021 cattgatgca gccatacccg tgcatattgt agccacatcg gaggtgatat actgcattgt
    10081 atcgtatatc cctagtccgg cataaacgct accgccgggc gagttaatgt atatctgaat
    10141 atccttcgaa ggatcggttg actccaggaa tagcagctgc gcctggataa tgttggccac
    10201 atcatcatct ataggcactc caaggaatat gattctatcc atcataagcc tggagaaaac
    10261 atccatggtt gcaacgttca gctgacgctc ctcaataatt gtgggtgaaa tgtagctgtt
    10321 ggttatcgtt ccaaagcgct gaagcgatag gctgctgata ccccgatgct tagttgcgta
    10381 tttttcgaat tcattcatga tgcctacttt ttaaatttag tttagtaacg caatttagca
    10441 cctaattgtt aaatgcggta gaactttcaa caaaaattag ctgagatgag gcaaaactat
    10501 ttaacctgct gttacgccag ccacctattt tcctgaaacc ggctccagct tatcaaattc
    10561 ctcatgcgta atctccttta catcaagcgg taggttttcc ttaaaccatg caatgacctt
    10621 gcggctgata atgccctcct gcattttacg tatttcatcc tggttcttca atatatcctt
    10681 ggcatagcgc tcaatgtact ctttcggaag gtagctcata ccgtaggcca taaattgctg
    10741 ctgggcgtat tccatggcaa aactttccat ctcttcgttg gtaacggtga agttttgctc
    10801 gctggcaatc ttgttgcgga ttagctgcca ctttaaatcc ccttcaaaaa gcgggtactc
    10861 gttttctacc tgctcccggg taaacttacc ctcgttgatg gtggtgagcc acctgattaa
    10921 aaaatccttt gggagctgca tgtcgatttt atcgagaaga atattccgaa gctcaacggc
    10981 aaagcgattt tcgccttcat tcttgagtcc cgattcaatt tcctcgtcaa ttttctggta
    11041 gaattcctcc tcggtggtaa ccaccccctc gccaaaaacc ctgtcgaaca gctcctggtt
    11101 aagctccgcc ggttggtagt tggaagtttt gtttacggtg agctggaaaa ctggctgaat
    11161 gttgggcaaa ttctcctttt gagtagaaag taatgaagcc ctgtcggcct cattggggaa
    11221 agctttttga acatcaatgg tgagtacatc acccacttta actcctacca gcttagcacg
    11281 ttcatcagga tcggcaacca gctcaaccga gatcgtggct ctttcaacaa acaaacctcc
    11341 ttcgcgagga gtttcatcac tattaagctc ggttaaagta gcctcgatta atcccctctc
    11401 gttggtctca tcggcttcgg caaaatgtcc aaaccgcatc aggtagctgt caatgtattg
    11461 cttgcgtatc ttgtcgtcag gggcaatatt gtagaatgga attttatcct ttttcccaag
    11521 cttaatctca aactcggggg ctactccaat ttcccatgcc agctcaaatt ccttatcggt
    11581 gtcccagttt atggtattct gcccctcgct gggcaatggc tctccaagaa tttttatttt
    11641 ctccttatca atataatcca ttacggcatc aaaggcaata tgcgtaacct cgtcaaccag
    11701 cacgtacttg tagtacatcc tatgtataag ggctgctggt gccatgcctg gcctaaaacc
    11761 tttaatgttt gagtttttac ggtagtcctt aagtttcttt tcaacctttt cgacatagtc
    11821 ggcctgctca acccttgccc gaatggaaat ggtttgttcg ttggtatttt ccttaactac
    11881 attcataata ttttgtatta caaccattta atggttggac atttaaaaaa ttaacctcca
    11941 tacagagggc gcaaaattag aaaaacaccc cgtaacaaca aaatggttac cgtaataagc
    12001 ttgtagccgg ggctaaaagt tgcttataaa atacgctgta catgttgatg aaaaccgtca
    12061 aagtggtata tttatcaaat aaaaaatgcc ctatgaactc cacagaattc catcgaaata
    12121 ttttgctggt gcttattctc ctcacatttt ccttgagcta ctcccaagct caatccgata
    12181 cggtcagaat aggagaaatg cttcagcgtg ccgaagtctt acgaaccacc aatccggatt
    12241 cggccatagg attggctatc caggcactta acatcatcga tggcagcgat aacaaggaac
    12301 tcatggtaag ggtagaacac actcttggaa attgctacta cagtcgaaac gattttgaac
    12361 tggctctttc gcatcttaag aaatccctgc agctagccac agaagaaggg cgaaagttct
    12421 gggttgccat gaacttgaac aggattggta atgtttacca gcttaaaagc gactatggca
    12481 gcgccctgca agcatacatg gaagctctga atattaacag gagtatcgac aataagagcc
    12541 agctggcccg aacgctggtg aacattggaa cagtttacag catcacgggt agctacaagg
    12601 aggccattga gaacatgctg aaggcgctgg aaacttacga gcaggcaaag gactacgagg
    12661 gaatagcatg ggcgtcgctg agcatatcga ggcttttttc ccgaatcgat ctttacgaaa
    12721 aagccctgca gtacgccgaa agctccctaa cccactaccg ccagctaagc aacataaacg
    12781 gagttactct ggccaaaaca gagctggcca atatttacta ccacttgggc atgtacccaa
    12841 aagctaagga gatggctacc gatgtgctgg cgacaaatca gcaaaatcaa aacatctacg
    12901 ggcaggctgc aaaccacctt ttactgggaa ttatcaactt tgagctgggc gattacacct
    12961 cagcaaaaag caacctgttg acctcgctgg agctaaagag acaggttaac gacagcattg
    13021 acctggccaa gctaagccgc taccttggag aggtgaacct gaaaagtggc aatgtggcag
    13081 caggtctgga atcattggaa caagccatgc ggattgccca aaggcaaaga gtgctcaccg
    13141 accagcgtga catattcctt agcctatccc aggcctaccg gttgaggggc gactacagaa
    13201 ggtcgctaga ctacttcacc cgctactcag cagtgaagga tagcattaac gccagccaga
    13261 ttgcccgcat ggagatgcag tacgattttg acaagcggga gcgggagcag gagcttatat
    13321 cccgccagcg tgaggccatt cagcaagcca aactcgaacg tcagcgtatg ttgacgctct
    13381 tcatctccat cgccctgctc cttgccgttg cgctggccgg ggtaatcttc tacttctacc
    13441 gtaacaagaa aaaaattaac gccctactcg tggcccagaa ccaggaaata ctcaggcaaa
    13501 aagaggaaat tgaggctcaa aaggaggaaa ttgaaagcca gcgcgacctg gctgaacgcc
    13561 aacgagacca aatagccggc cagcaaaagc aaattaccga cagcatcagg tatgccagcc
    13621 ggatacagtc ggcagttatg ccccgtaccg ataccctggc aaccttcctg accgactact
    13681 tcatttactt ccaacccaaa aacattgtca gcggtgactt tttctgggtt acccgtttga
    13741 acgacggacg tatagcaatt gctgtagccg actgcaccgg tcatggggtg ccaggggcct
    13801 ttatgagcat tttgggcatt accctgctaa aggagattat agcctaccaa caagttaaca
    13861 ctgctggcga tttgcttgat aaacttaggc agatggtaat cgccgctctg aaccagtcgg
    13921 gacaggtgca ggattcccat gacggaatgg atatggggct gctggttgtt gatgccaagg
    13981 gcaggaaagc agaatacgcc ggagcctaca tgccgttact tgtttccaga aactccgaat
    14041 tgcctgaggt tagcgctggt gttcaacccg aaacagccaa cggatggtca atttatgaga
    14101 ttaagggtga caaaatgccc gtaggttacc atctggcagg agaacgcccc tttacaactc
    14161 acaggttcga cctcctctcc aacgacacat tctaccttat gtccgatggt tacacagacc
    14221 agtttggcgg agcattgaac caaaagttga tgatggtcaa cttcaaaaaa aatctgcttg
    14281 gtctccagac gctcagccta tcaaggcaaa acgaagagct acagaaacaa ttaatagaat
    14341 tccagggcac acaaaagcaa gtggatgata tcctggtgat gggttttagg ttatagctgg
    14401 taaacatacc cataaaatag ctaccgctaa agcgagcgca gcaatgtttt tacctattcc
    14461 ctagtttaat gccaaaatcg cattaatccg atagctttag gtgagccaaa tctgctacat
    14521 ttgcaacccg ataaaccatt ggttactttc accctcaaat aatggcaggg gaatatccat
    14581 gaggtacatc aagtaaaaaa actaaccgga ccacgctagc cggacctaat gggttaatgt
    14641 ggagaaaatt gcgtaatgga taaacaatag ataacataag ggtatgggaa tgtctaataa
    14701 aaagtttttg gtttacattg ccgtcacagt tgcaatggct ttttgggcgc tttcttttat
    14761 ctggtacaaa gatgcctatg tttactacag gcctattacg ctagtgttct tccgtctggt
    14821 aatttcagcg gcattccttt acgttttttc actggctatg aggaagctag aacgggtaaa
    14881 aaaaggcgac ctgaagctgt ttttcctgct ggccttcttt gagccgcttc tatactttat
    14941 tggcgagagt tttggtatga cccttatctc ctccaccatg gctgccgtaa ttgtttcaac
    15001 cattccccta cttagcccag ttggagaatt tttcatgttc catcgcagga tttccaccac
    15061 caacctcatg ggaattattg cctccatgct gggcgtatgc ctggtaatct ttcataacgg
    15121 atttgggcag gtggatgtta atttcaccgg ggttgccctg atgtttctgg cggttttttc
    15181 aacagttggg tactcgttga ttgtaggtaa gctaaccgta aagtacaacg tttacagcat
    15241 aataacctac cagaatacca ttggggcact ttacttcatg ccgttattcc ttatttttga
    15301 cttcaaccac ttccaaactg taggtattag ctggcaggct atggtcccgg ttataaaact
    15361 tggaatattt gcatcaactt ttgcatttat gcttttcacc tattccatca agcacttggg
    15421 aataaccagg gcaaacactt tcagtaacgc cattccggtg ctaacggcca ttttcgccta
    15481 tttcctgctc gatgaagccc ttacacctgt gatgattgcc ggtattctgg tagtggtatc
    15541 gggccttttc ctttcacaga tttcacctaa gaccaaaact cagatagagc taaaggtttt
    15601 gccggagcat ccggttgttg gcattggcct acggttgcga aaactattca gaaagtagcc
    15661 gggtaaattc gctaagccta acgactattg actgctatgt gaattccaaa ttaaaaagcc
    15721 cctcaaaagg cctgctgcat taaagaactg ctaacttcat tgttgaaatt aaccagccaa
    15781 acattacgct tatacagctt caccaaaaca ttcacatgaa aacaagctca tcaaaaagca
    15841 taacaagtaa tcggtcgaat agtgttggct gcgatgcatc agtagctgaa ccaccaacta
    15901 aatattgctt acctggtctt tagcgatcga ttctgtgaat ttcagcgcca atggcattaa
    15961 gcctgccgtc aatgttttgg tagccgcggt caatctgctc gatattatga ataatgctct
    16021 taccatcggc cgacatggct gcgataagca gggctacccc ggcccgaata tcgggcgagg
    16081 tcattacggt aggcctcagc ctaagctgcc tgtcgtggcc aataacggtt gccctatgcg
    16141 gatcgcaaag aatgatctgc gctcccatgt ctattagctt atcaacaaag aagaggcggc
    16201 tctcgaacat cttctggtga atcagcaccg agccctttgc ctgggtggct acaacaagaa
    16261 aaacgctgag caaatcgggg gttagccccg gccaaggggc atcggcaagc gtgaggatgg
    16321 acccgtcaat aaaggtgtca atctcgtagt gctcgtgggg agggatgtga atatcgtcgt
    16381 tggtaacctc gaagtttatc cccattcggc gaaaggcatc gggaatgatt cccaggttgc
    16441 cgaatgagac attgcgaatt gtaatatcgc tgcaggtcat ggcagccagc ccaataaacg
    16501 agccaatttc gatcatatcg ggcagaatgg tgtgctcgca tccaccaaga ccatctaccc
    16561 cctcaatggt cagcaggttg gagccaattc cagaaattct ggcgcccatc gaaaccaaca
    16621 tgtggcaaag ttgctgaaca taaggttcgc aagctgcatt gtaaatggtg gtttttccgg
    16681 gagtcatggt ggcagccatc aggatattgg cggtaccggt aaccgatgcc tcgtcgagca
    16741 gcatgtagga acccttcagg tgtttcccct ccagggtgaa aaagttgtcg ttaacatcga
    16801 agtcaaactc cacccctagc ttctgaaagc caagcaggtg ggtgtcgagc ctacgccttc
    16861 caatcttatc acctccgggt ttgggtgcat atgctttgcc atagcgggca agcaagggac
    16921 caaccaccat gattgaccca cggatggcag ctgccttaac cttgaaatcg tcagtggtaa
    16981 gataatcaat gtcaatattt ctggctataa atgaatacga cgactcggat aagcgggtaa
    17041 cctcgacccc cataccctga agcagctcga tgagcttcac cacatccctg atggcaggaa
    17101 tgttgtttat ggtaatcttc tcgggggtta gcagggtggc gcaaataacc tgtagcgcct
    17161 cattcttagc tccctgagga aataagtcgc cttttagacg cttaccgcca attacctcga
    17221 acgttgccat gccaccctaa tgttttttat ggtacttggt gcggggtttg tttttaaatt
    17281 ccttgttatc caccagcttt gatccgggag ccattaccaa catgccgttg cttagggtct
    17341 gcatatcgcg aaaaatatca tcatcgctca ccgactcctt gttccatgta atgttggcct
    17401 ttttcatctg gttggcaata agctgcttga gggcatcctt ctcgggtcct tcctccattg
    17461 ctacagcttt ttcaatcatc atttcaatta ttcgcccgta atgcttaaac ttgatagggt
    17521 tacccgggta tggaacccta cggggctttt cgttgtactg ctccacgctg ggaatggggt
    17581 atggagagtc tatatcaagc ttgaaatcgg acatgatgaa caggtgatcc cataacttat
    17641 gtttaaagtc gttaatatcc ctgagatggg ggttaaggtt ccccattatg gcgacaaggg
    17701 attttgccag cctgttgcgc tcgtccctat cttcaacgga tgcaatgtag tcaaccattt
    17761 gctgtatgtg ccttccgtac tccggaagag cgagtttctt tcgctgggtg ttgtagtcca
    17821 ttagaaatat ttaaaaggta aaaataaaaa atttcgggat gtatgtaata aatagctggc
    17881 ggtaagccta atgcagtaca tcattttatg ctgcaaagtt aggttatcgg ttgtaaaaat
    17941 gcaaactgcc tgaaagggtt aagcatcctc acgtgactta agggattgaa aaagttggca
    18001 cctgcagcat cagcctttcc ggttaagctg aagctaagct attcccttca ggttggcaaa
    18061 cttaatgaga aggttttttt ggcctgcctc cgtgaatagc acggttgcct ttttgttagc
    18121 gccttcgcct tcgacgctta aaaccttacc ctggccaaac tttccatggt aaacctccac
    18181 gccttcgcaa atttcctcgg gtggaagctc ttttaggttt tccggaacgg atacgggtgt
    18241 cctgcccggg ggctgtgaat agggagcaac ccgatttcca attgagtttt tcccaaaatt
    18301 gtttatgctg gtctgtcggg atagctcact ggttgctttt agatgacctc caccatcagg
    18361 ttcaaagggg gattcaacgt agcgctgatc aatctcgctt ataaaccggc tgggattgga
    18421 aatgttaatg tttccccatt tgagccgggt ttgggcaaat gaaatggtta cccccttggc
    18481 tgcccgggta agcgcaacat agaacaaccg gcgttcttcc tccagctcct ccggcgtgtt
    18541 catggacatg tttgaaggga aaaggttctc ctccagccct gttaggaaaa cgtaatcaaa
    18601 ctcaagcccc ttagccgaat ggatggtcat aacgctaata cggttccggt cgttggggtc
    18661 atcgttgtcc aaggaggtga gcagtgcaac attttcaagg tagtcaacaa gcgtagtggt
    18721 ttcgtcaata ccttcattcc tgcgcgcctc aataaactcc ctgatgc
//
LOCUS       DAOPHX010000041        17616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_3588_length_17616_cov_6.125847, whole genome shotgun sequence.
ACCESSION   DAOPHX010000041 DAOPHX010000000
VERSION     DAOPHX010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 17616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17616
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3588_length_17616_cov_6.125847"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /gene="rlmB"
                     /locus_tag="PK502_04670"
     CDS             complement(<1..669)
                     /gene="rlmB"
                     /locus_tag="PK502_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPH00144.1"
                     /translation="MDNQKDSPIYGIRPVMEALESGMKISRVVVKQGLSGSLVSELMV
                     LLKQRNVPVQYVPLEAFDKFKDKNHQGVVAFSSPVEFQNLEHILPKLLEREIKPFILM
                     LDGVTDVRNFGAIARTAECAGVDAIVVPAKGAASMGSDALKTSAGALNHIPVCRVGSL
                     KITINLLKQHGFRVIAATEKAQKSPFEANLTGAVAIVAGSEDTGISPDIIKLADELVR
                     IPIFG"
     gene            complement(681..2639)
                     /locus_tag="PK502_04675"
     CDS             complement(681..2639)
                     /locus_tag="PK502_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPH00145.1"
                     /translation="MISVNQLTLSFGGFELFKEISFQLNEGERVGLVGRNGAGKTTLL
                     KIIAGEMQPTSGRVSMPGSVSIGYLPQQMALPSGRSVMDETLMAFSETLELSRSIDKI
                     GEELSGRNDYESAGYLQLINRLSELTDRFNLLDGGTANAKAEQTLLGLGFRRSDFDRT
                     TGEFSGGWRMRVELAKILLRKPQVLLLDEPTNHLDIESIQWLEDYLKDYQGSLLLISH
                     DRAFLDNVTQRTVELSLGKLYDYKVPYSQYVELHRLRREQQMAAYRNQQKVIEDTNDF
                     IERFRYKATKSVQVQSRIKMLERMEIIEVDEDNTASVNIRFPSAPRSGDVVCEAKDVA
                     KSFGSKKIFGGVNFTIERGQKVAFVGRNGEGKTTMSRIITGELSHEGSLRIGHNVAIG
                     YFAQNQDELLDGSLTVLETIDRVAVGDIRTRMRDILGAFLFRGDDVDKKVSVLSGGER
                     NRLALAKLMLQPYNLLVLDEPTNHLDMHSKEVLKSALMQYDGTAILVSHDRDFLDGLV
                     GKVYEFSDGAVREHIGGIYDFLQRKKLESLKQLEHKARVDAQTTSSGNGSDLKRLWSE
                     RRELDREIRNMENSLKQVESKISAMENEIDDINRKLAEPDSHSINLSDSDFFAGYENK
                     KRQLLDYMQRWEELSLKLEELKLKRNAL"
     gene            complement(2800..3513)
                     /locus_tag="PK502_04680"
     CDS             complement(2800..3513)
                     /locus_tag="PK502_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HPH00146.1"
                     /translation="MEWIERTELLLGAAKLQKLSRAHVLVVGLGGVGAYAAEMICRAG
                     VGQLTIADGDTIHPTNINRQLVALNSTVGLPKAEYMGKRLLDINPDIKLMVVPKYLRD
                     DEMKYIINQPYSYVVDAIDTLSPKIFLIRDALEYGHRLVSSMGSGGKLDPTMVSIADI
                     SETHSCPLARILRKRLHRLGIRKGFKVVYSPEETPENATRPCVGEPNKKTTVGTISYM
                     PAVFGCFIASVVIRDLLAD"
     gene            complement(3604..4269)
                     /locus_tag="PK502_04685"
     CDS             complement(3604..4269)
                     /locus_tag="PK502_04685"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPH00147.1"
                     /translation="MPLINFHTHLTSQKEGEVRIVSLNVEEVDNIESLSMVTVGVHPW
                     QTDSNNMEDLLAKFHELIRHPNVIGIGEVGLDRLRGGSLTLQAKYLREQAIAAASISK
                     PVVIHCVRAWSELVETLSAPDLKQLTKAIHGFRGKADLARQLVDRNYYLSFGSILVDP
                     SPELAESLTVIPKNRLFLETDTSEMPIGEIYSAAADILDTSVDELTLQVENNLNTFFG
                     FNG"
     gene            complement(4330..5568)
                     /gene="pepT"
                     /locus_tag="PK502_04690"
     CDS             complement(4330..5568)
                     /gene="pepT"
                     /locus_tag="PK502_04690"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HPH00148.1"
                     /translation="MINKQSLIDRFLRYVAIDTQSDDTVSDRFPSTEKQLVLSNLLVK
                     ELKELGVSDATIDEFGYVMGTIPANSTKKVPVIGFLAHVDTSPDMPGKNVKPRFVESY
                     DGSDITLNAEKGVILSAREFPELKEYTGQTLIVTDGTTLLGADDKAGIAEIMTAVEYI
                     TGNPTLRHGKIRIGFTVDEEVGRGVDHFDVKRFGADYAYTLDGGAVGELEFENFNAAS
                     AKITIQGRNIHPGYAKDKMLNAILIAQEFNSMLPPHESPEHTEGYDGFYHITKIEGTV
                     EKASFQYIIRDHDMRKFERRKAYMERVTRYLNSKYGDQTVTLEINDQYYNMREKVEPV
                     YHVVQTAIEAMEKVGIKPKVRPIRGGTDGARLSYMGLPCPNLFAGGENFHGKHEYVPV
                     ESMVKATETILKIVEIYESR"
     gene            complement(5814..9083)
                     /locus_tag="PK502_04695"
     CDS             complement(5814..9083)
                     /locus_tag="PK502_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPH00149.1"
                     /translation="MKKVCSMMLLLGLLSVGLAGAQEARLMRFPAVHGNQVVFTYAGD
                     LYTVPLNGGVARKLTSHIGYEMFARFSPDGKTIAFTGQYDGNTEVFTIPAEGGTPKRL
                     TYTATLSRDDISDRMGPNNIVMTWTPDGKNIVYRSRKQSYNDFVGSLFMVPIDGGLSS
                     ELPLTGGGFCSYSPDGKQLAFNWVFREFRTWKYYKGGMADDIRIFDFDTKQITNITNT
                     NTQEIFPMWIGGKIFFLSDRDRTMNLFCYDLKTQETKKVTNFTDYDIKFPSFSGNTIV
                     FEKGGYLYSLDASSLAANKIDVTIADDQLYSRSELVDAGKSIRSMDLSPNGERVVFSA
                     RGDVFTLPAESGITRNLTQSSDAHDRNAAWSPDGKYIAYISDKSGEYEIYLQAQDGAS
                     APVQLTTNTNNYIFNIEWSPDSKKILFTDRLSRLQCVDVETKAVSQLAKGKYGSIYSY
                     SWSPDSKWVAYSTEDENRFSVVFLYNTETASTYNVTGNWYTSTNPVFSNDGLYLLFVS
                     DRDFNPIYSSTEWNHAYTDMSKIFMVMLSKNTPSPFAPENNEVKIEPKPVTDAKKKNA
                     DDKKATEAQPTVKPVQIDIDGIVDRTVSLPIDAANYRVAACIDGKVYYSYYGSGRGSQ
                     VARVYDLKKKTETDLGESLQFAISANGKKMLVSKAGKYSVIDLPSSKVNMEKTISLNS
                     MKVWVDYRQEWKQIFDESWRQMRDFFYVENMHGLDWKAIHDKYAVLVPYVNHRNDLTY
                     IIGEMIGELSIGHAYVNSGVKPEPERTNTGLLGAKVSKDASGYFKIDRILEGASWDKS
                     LRSPLADIGVNVKTGDYILAVDGADLKNTPDIYAKLIDMADKTVELTVNSKPELNGSR
                     KVLVVPVANEANLYYYSWVQNNIRKVSEATNGQVGYIHIPDMGVEGLNEFVKYFYPQL
                     TKKALIIDDRGNGGGNVSPMILERLSRVAYRQTMRRGISYASPIPSETHVGPKVLLID
                     RYSASDGDLFPYGFKKLKLGPVIGVRTWGGVVGISGSLPFVDGADLRKPEFASFSSDA
                     SEWIIEGHGVDPDIVVDNDPYQEYMGVDTQLNKAIEVALDKLKDFKELPPIPPAPDKS
                     K"
     gene            9382..12432
                     /locus_tag="PK502_04700"
     CDS             9382..12432
                     /locus_tag="PK502_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPH00150.1"
                     /translation="MRHIGYFLLAATAALALPSSSNAVGLKNCHSGNGSAEKKIQETW
                     ADSVLSSLTLRERIGQLFMVAAYSNRDDMHVREIADLVRKDGVGGLIFFQGGPVRQAN
                     LTNYYQSLAEVPLLVGMDAEWGLGMRLDSTFSYPRQMMLGAAQDSALVYEMAADIARQ
                     LKRMGVHINFAPVVDINSNPQNPVINTRSFGENVNLVSRYGKAYVSGLQDNGVMACAK
                     HFPGHGDTFEDSHFSLPKVPHSKQQLDSVELYPFKQLISYGVQSVMVGHLRVPAMEND
                     TLLASSLSPAIISGNLISEMGFKGLIFTDALNMKGVADYFSPEDINLKALLAGNDVLL
                     FPSEVSRSIDKIEKMVRRGQIPEEVINIKCRKVLMAKYAAGLSSYKPIEVLNLYSDLN
                     RVSSEVIRRKIISRALTVVHNNNSFIPVKRLDTLRIAYLEIGCNRGDAFREQLELYTA
                     IHTFSIDPSSPAEEFDSLLNILEPYNLVIAGYHATDSRFSMNYGVTCQAANFLFDLAF
                     SSAVVLDIFGSPYAISKFYNPDSFRAIIVSYDNSFDAQNLSAQLIFGGRFATGRLPVT
                     ASAWYSAGYGVTTINPIRLGYALPEEVGINSLLLKEVDSLANSAIELAVAPGMQILAA
                     KDGVVFYHKSFGKPTYLSSDSVDVRMLYDVASVTKVVATVPSVMRLYDEQALELDNNL
                     GAYLDLKEFPDKSAIRLSDLLRHQSGLKSWIPFNLKTLTTMIPNIPLWQEKLSTDYPF
                     QLGSKQFFSRYTVPNPVFYCDKESRDFPNRCAEGLYSAYFMADTIFSAINRSELSQPG
                     KYVYSDLGFMYLQRVVEGITQTGIDRYCEDKFYSRLGMSYTLFKPLTRFDRDRIVPTE
                     VDLIFRRQLVWGDVHDPAAAMLGGVSGHAGIFSTANDLAKLMQMFLQDGTYGGERFFR
                     PGTVEQFTTAPIEANSSDNRRSLGFDKPASEGPSPAGSLASSQSFGHTGFTGTTVWAD
                     PENGLVYIFLSNRVYPDASNTKLAEMNIRTRIHDVFYRAIGVAK"
     gene            complement(12592..15411)
                     /locus_tag="PK502_04705"
     CDS             complement(12592..15411)
                     /locus_tag="PK502_04705"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPH00151.1"
                     /translation="MKKTRFYLCALGILLGFSAGAQDQVNWNTPIPLDRNVRFGQLEN
                     GLTYYIRKNTEPQQRAEFFIAQNVGAILEEDNQNGLAHFLEHMAFNGTKHFPGKGIIN
                     YFETVGVKFGFNINAFTSLDETVYYLSDVPTNREGIIDSALLVLHDWSGFISLEPDEI
                     EAERGVIREEWRTGRNAERRMYKQVMPVIYKGSKYAERDVIGDINVINNFKHEEIKNY
                     YHKWYRPDLQSIVIVGDVDVDQVEQKIKTLFADIPKRENPDVRPFFELPDNDEPLIGI
                     ASDPEARNTILTVYYKHQAVPKNEKNLGYVREQMIQKLATSMISARLNEITQKPNPPF
                     VFAASDMGNMVRTKSALMFYAALRNDAMIDGIKGLIREAERVKQFGFTATEFDRAKSD
                     YMRGLENELKEKDKQKNQKYVWEAINNFLEGEPMPGIEFEFMLANGLVPTITLEEINA
                     YIASMVTDKNVALTLMAPKKDDIQLPSEEAILAAVKEVKSETLQAYVDNVVMKPLVES
                     IPTPGQIKKVNDKNIFGTTELTLSNGVKVILKPTDFKEDEVKLDAFSKGGLSLADLAT
                     LPSATLATDMVVNAGVGEFSSVDLEKLLAGKMVRVNPVFGEDFEGFQGSASPKDFETM
                     LQLVYLYMTAPRQDQENFNTYLDRLKAFFANAAADPRMAFRDSVNTAMANHNPRVMPI
                     NTDFLSKVDFNKCYSFYRDRFADASDFTFIFTGNINVDEAKALTAKYLGALPVVKRKD
                     IPRDNGVRPPKGKVQNYFTKPMQTAKSSVFVGFTGNAEYNLKNHVMAEYLTSILRTRY
                     LEEIREKEGGTYGVGVNISIDNFPTQSFTAGIQFDTDPKLRDKLIGIVYAEIEKIKQN
                     GPTPDDLNKTKEFLMKEYEQNQRENSYWSDAIRMKYEDGVDFNTEYLNVVKSTSVESL
                     KDFAQKLFNQNNVVEVVMTSEEAQK"
     gene            15884..17077
                     /locus_tag="PK502_04710"
     CDS             15884..17077
                     /locus_tag="PK502_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPH00152.1"
                     /translation="MKKFLTRTIVMLSLVSLFTDIASEMLYPIMPVFLKSIGFSVLLI
                     GILEGLAEATAGISKGYFGHLSDRLGTRVPFIRWGYTLSAISKPLMAVFTWPIWIFGA
                     RTLDRLGKGIRTSARDALLSDESTRETKGKVFGFHRGMDTLGAAIGPIAALIYLHFYP
                     QNYSTLFLIAFAPGLVAVSLTLLLKDKKKPETSNQQVNPVQGKGFFSFVKYWGHSPKA
                     FRYLVVGLLFFTLFNSSDAFLLLMVKEKGFSDANMISLYIFYNLLYALLAYPMGWLAD
                     KFGMKRIMIFGVSVFGLVYLLFGFADKLHMFLLLFGLYAFYAAATEGVSKAWITNIVP
                     GESTATAIGFYTGFQSVATLLASSMAGLIWTKFGASTVFVISGLAAFITAIYLMLATR
                     MNKLA"
     gene            complement(17069..17533)
                     /locus_tag="PK502_04715"
     CDS             complement(17069..17533)
                     /locus_tag="PK502_04715"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HPH00153.1"
                     /translation="MIQRIQSLYLLISAILLAFFFFMPYAHYAVGPQEVKISLSASGL
                     GQDGSVVENLPKLWAVLALVILAFTATLVTIFLFKKRKLQARLCIFNIVLLIGLQGLL
                     FYIAKAVEQRLMAPPSYGLIFIFPLVCAIFTYLAYRAIAKDEALIRSLDRLR"
BASE COUNT         4324 a         4216 c         4172 g         4904 t
ORIGIN      
        1 cccgaatatg ggtattcgca ccagctcatc ggcaagcttg ataatatcgg ggctaatgcc
       61 ggtatcctct gaacccgcta ctatggcaac tgcccccgta aggttagcct caaagggaga
      121 tttttgggct ttttcggtag cagcgataac tctaaaacca tgttgtttca gcaggttaat
      181 ggttattttt aggctgccta cccggcatac cgggatatga ttcagcgcac cagccgaggt
      241 ttttagggca tcgctaccca tgcttgcagc accctttgct ggcactacaa ttgcatccac
      301 tcctgcacat tcggcagttc gggcaattgc gccaaaattc cgaacatcgg ttaccccgtc
      361 gagcataaga ataaatggtt ttatctccct ttcaagcagc ttgggaagga tatgctcaag
      421 gttctgaaac tctacgggcg acgagaaggc cacaaccccc tggtgatttt tatccttaaa
      481 cttgtcgaac gcttcaagcg ggacgtactg gacaggaaca ttcctttgct ttagcagcac
      541 catcagctcc gacaccaggc tgccgctcaa cccctgcttt acaactaccc tgcttatttt
      601 cattcccgat tccagagctt ccatcaccgg acgaataccg tagattgggc tgtctttttg
      661 attatccata gctgccagtg ttaaagtgca tttcgtttca acttcagctc ctccagcttt
      721 agggagagct cctcccagcg ctgcatgtaa tcaagaagct gccttttttt attttcgtag
      781 cctgcaaaaa agtcggaatc cgacaggttt atgctgtgtg agtccggttc ggccagtttg
      841 cggttaatat catcaatctc attctccatg gccgatattt tgctttcaac ctgcttcagg
      901 ctattttcca tgtttcgtat ttcgcgatca agttccctgc gctccgacca tagcctttta
      961 agatccgatc cattgccgga gctggtagtt tgagcatcaa cccgagcttt gtgctccagt
     1021 tgtttcaggc tttccaactt cttacgttgg aggaaatcgt agattccccc aatgtgctca
     1081 cgcacagcac catcggaaaa ctcataaacc ttgcctacaa gtccatccag aaaatctctg
     1141 tcgtgtgaaa ccagtattgc cgttccatca tactgcatca gggccgattt gagcacctcc
     1201 ttcgagtgca tgtcaaggtg gttggttggc tcgtcgagga caagcaggtt gtagggttgg
     1261 agcatcaact tagccagggc caggcgatta cgctcaccac cggaaaggac gctgactttt
     1321 ttatctacat catcacccct gaaaaggaaa gcgccaagaa tatcgcgcat cctggttcgg
     1381 atgtccccaa ccgcaaccct gtcaatggtt tcgagaacgg tgagggagcc gtcgagcagc
     1441 tcatcctgat tctgggcaaa gtagccaatg gctacattat ggcctattct aagtgagccc
     1501 tcatggctaa gctcgccagt tattattcgg ctcatggtag tttttccctc cccatttcta
     1561 ccaacaaaag ccaccttttg cccgcgctcg atagtgaagt tcaccccgcc aaaaattttt
     1621 ttggaaccaa agctttttgc cacatctttt gcctcacaaa ccacatcgcc cgagcgggga
     1681 gcagagggga agcggatgtt taccgaagca gtattatcct catcaacctc aataatctcc
     1741 atcctttcga gcattttgat gcgcgactga acctgaacgg acttggtggc cttgtagcga
     1801 aaacgctcaa taaaatcatt ggtatcttca attacctttt gttgattccg gtaagcggcc
     1861 atttgctgct cacgccttag cctgtgaagc tctacatatt gggagtaggg aactttatag
     1921 tcgtacagct tgccaagtga aagctcaacg gttcgttgcg ttacattgtc aaggaaagcc
     1981 ctgtcgtgcg aaatgagcag cagcgatccc tggtagtcct taaggtagtc ctccagccat
     2041 tgaatagact caatatcaag atggttggtg ggttcatcaa gcaggagaac ctgtggtttg
     2101 cgaagtagaa ttttggcaag ctcgacccgc attctccatc caccgctaaa ctcgccggtt
     2161 gtcctgtcga aatcgctacg cctaaatcct aacccaagta acgtttgctc ggctttagca
     2221 ttggccgttc cgccatccag caggttaaac ctgtcggtta gttcagacaa gcggtttatc
     2281 agctgcaggt agccggcaga ctcgtaatcg tttcttcccg agagttcctc acctattttg
     2341 tcgatgctcc tactcagctc gagggtttcg gaaaatgcca taagggtttc gtccataacg
     2401 cttctgcccg atggcaatgc catttgctgg gggaggtatc caatgctaac gctgcctggc
     2461 atcgaaacgc gtcctgaggt tggctgcatt tcgcctgcaa taatctttag tagggtagtt
     2521 tttcctgcgc cgtttcggcc aaccaaccca acccgttcgc cctcgttaag ctgaaagctg
     2581 atttccttga aaagttcaaa acctccgaag ctgagggtta gctggtttac tgatatcatc
     2641 tgagactact cgaaaagtta ttggctgcaa agatactaat ttcgcttcac cctgccatca
     2701 atggcattat tgtgccctcc agaccgtgaa gctgtggatg tgcagttgtg atgatgttga
     2761 tggaggagga gtgattgcgt taaccacaac cattgtgggt tagtcggcca gtaggtcacg
     2821 tatcaccacc gaagcaataa agcacccaaa aacagcaggc atgtaagaga ttgtgccaac
     2881 agtagttttc ttatttggtt cacccacaca tggtcttgtg gcgttttcgg gcgtttcctc
     2941 aggggagtaa accaccttaa atcccttacg gatacccaac ctgtggagcc gtttgcgaag
     3001 aattcgggca agcgggcagc tgtgggtttc cgaaatatcg gcaatggaaa ccatggttgg
     3061 atcaagtttt ccaccgctac ccattgagct tacaaggcgg tgtccatact ccagggcgtc
     3121 gcgaatcagg aatattttag gtgagagggt gtcaatggca tccaccacgt agctgtaggg
     3181 ctgattgatg atgtacttca tctcatcgtc gcgcaggtac ttaggcacca ccatgagctt
     3241 aatatcaggg tttatatcca gcaggcgttt tcccatgtat tctgctttgg gcaggccaac
     3301 cgtgctgttc agggcaacca gctgcctgtt gatgttggta gggtggatgg tatcgccatc
     3361 ggctattgta agctgcccaa ctccggcgcg acaaatcatt tcggcagcat aggcgccaac
     3421 accgccaagc ccaaccacca gcacatgcgc ccggcttaac ttctgcagtt tagcagctcc
     3481 aagcaggagt tcggttcgct caatccattc cattgctgta agttggtaga gtgctcataa
     3541 agttgctaca tttcctcggg tgtgaggcaa ggggtgtttt agccgggtaa ttttacatgg
     3601 caattatcca ttaaacccga aaaacgtatt caggttattc tcaacttgca gggtaagctc
     3661 atcaactgag gtgtcgagga tatctgcagc ggcggagtat atctccccaa taggcatttc
     3721 ggaggtatcg gtttctaaaa acagcctgtt tttggggata acggtaagcg attcggccag
     3781 ctcgggcgat gggtcaacca gaattgaccc aaaagagagg tagtagttcc tgtcaaccag
     3841 ctgcctggca agatctgcct tgcccctgaa tccatgaatg gcctttgtga gctgtttgag
     3901 gtcgggtgca gaaagggttt caaccagctc gctccatgcc ctaacacagt gaatgactac
     3961 aggctttgaa atgctagctg ctgctattgc ttgctcccga aggtattttg cctgcagggt
     4021 taaacttcca ccgcggagcc gatcaagccc aacctcgccg atacctatca cgttggggtg
     4081 tcgtataagc tcatggaact tggccagtaa atcctccatg ttgttgctat cggtttgcca
     4141 tggatgaaca ccaaccgtaa ccatgcttag cgattcaata ttatcaactt cttctacgtt
     4201 gagcgaaaca atccgtactt cgccttcctt ttggcttgtg aggtgggtat gaaagtttat
     4261 gagcggcatt ctgcttacag gataaatgtt gtttacggtt gaagtgtatg cgggttgaaa
     4321 cttaaatatc tatcttgact cgtaaatttc aacaattttc agtatggtct cggtggcctt
     4381 caccatgctt tcaaccggga catactcgtg ttttccatga aagttttcac cgcctgcaaa
     4441 caggtttggg caagggagtc ccatgtagga aagcctggca ccatcggttc caccgcgtat
     4501 gggtcgaacc ttaggcttaa taccaacttt ttccattgcc tcaatggcgg tctgcaccac
     4561 gtggtaaaca ggttccacct tttcgcgcat gttgtagtac tggtcgttaa tctcaagggt
     4621 aaccgtttgg tcgccgtact tactgttaag gtaccgggta acacgctcca tgtatgcctt
     4681 gcgtctttca aacttcctca tgtcatgatc gcggatgatg tactgaaatg aagctttctc
     4741 aacggttccc tcaattttgg tgatatggta gaaaccatcg tagccctcgg tgtgctcggg
     4801 gctctcgtgg ggcggtaaca tgctgttgaa ctcctgggca ataagaatgg cgttaagcat
     4861 tttatcctta gcatagcccg gatgtatatt ccggccctga atggtaatct tggcactggc
     4921 agcgttgaag ttctcaaact ccagctctcc aacagcacca ccatcaagcg tataggcata
     4981 gtcggcgccg aaacgtttca catcgaagtg gtcaaccccc cgaccaacct cctcgtcaac
     5041 cgtgaagcca atacgaattt taccatgtcg taaagttgga ttgccggtga tgtactcaac
     5101 ggccgtcatg atttcagcta ttccggcctt atcgtcggct ccaagcaggg ttgtgccatc
     5161 ggtaactata agtgtttgcc cagtatactc cttgagctcg gggaactccc tggccgatag
     5221 aataaccccc ttttctgcat tcagggtaat gtcggagcca tcgtagctct caacaaagcg
     5281 tggcttgaca tttttcccgg gcatatcggg cgaggtatca acatgtgcca gaaagcctat
     5341 aacaggaact ttctttgttg aattggcagg aatggtgccc ataacgtagc caaactcgtc
     5401 gattgtcgca tcggatacgc caagctcctt taactccttc accagcaaat ttgatagaac
     5461 cagctgtttt tcagtgcttg gaaaccgatc ggataccgta tcgtcgcttt gcgtatcaat
     5521 agctacgtac cttaaaaaac ggtctatcag ggactgctta tttatcatgg cttgagtatg
     5581 ttaaaaagca aatttgtttt ttagaaaaat aatgacgtct tagaaaaaat agcctttaaa
     5641 tttaggttct ttgggacaac tatgcataaa aaaatgaaaa taatttactc gtttatgcta
     5701 gtaggttgat aaatagaagg ttatagccat gctggaagtt gatagtagtt aaaaaacaaa
     5761 gcggctcatg ttaagccatg gccgctctgt agttttgtat cttgttgttc cggctacttc
     5821 gatttatcgg gtgctggtgg aattggtggc agctccttaa agtccttcaa cttatcaagt
     5881 gcaacctcaa tggccttatt cagctgggta tcaacgccca tgtactcctg gtaggggtcg
     5941 ttgtcaacca caatatcggg gtcaacacca tgtccctcga taatccactc gctagcatcg
     6001 ctgctaaagc ttgcaaattc cggtttacgc aggtcggcgc cgtcaacaaa tggaagtgag
     6061 ccggaaatac caacaactcc accccaggtt cgaaccccaa taaccgggcc gagtttcagc
     6121 tttttaaatc cgtatgggaa taaatcgcca tcggaagctg agtagcggtc gatgagcagc
     6181 accttaggcc caacatgtgt ttcggatggg atgggcgagg cgtagcttat accccgccgc
     6241 atggtttgcc ggtaggcaac acggctaaga cgttcaagaa tcatgggcga aacattacct
     6301 ccgccgttac ccctgtcgtc aataatcagc gctttcttgg ttagctgggg gtagaagtac
     6361 tttacaaact cgttaagccc ctcaacgccc atgtcgggaa tgtggatgta gccaacctgg
     6421 ccattggtgg cctcggatac cttgcgaata ttgttctgaa cccagctgta gtagtacagg
     6481 ttggcctcgt ttgccacggg aacaaccaac accttccggc ttccgttcaa ctcgggtttg
     6541 ctgttcaccg tcagctcaac cgttttatcg gccatgtcaa tcagttttgc gtagatgtcg
     6601 ggggtattct tcaggtcggc tccgtcaact gccagaatgt agtcgcctgt tttaacatta
     6661 acgccgatat cggccagggg cgaacgaagg gacttgtccc agctggcacc ctcaagaatt
     6721 ctatctattt tgaagtaccc gctggcatcc ttgctcactt tggcgccaag caacccggta
     6781 ttggtgcgct cgggctcagg ttttaccccg ctatttacat aggcatggcc aatgctcagc
     6841 tccccaatca tctcgccaat gatgtaggtt aagtcgttgc gatggtttac atatggaacc
     6901 agcacggcat acttatcgtg tatggctttc caatccagcc cgtgcatgtt ttcaacgtag
     6961 aagaaatcgc gcatctgacg ccagctctcg tcgaaaatct gcttccactc ctgacggtag
     7021 tcaacccaca ccttcatgct gttgaggctt atggttttct ccatattcac cttcgaagag
     7081 ggtaaatcga taacgctgta ctttccggcc ttggatacca gcatcttttt tccattggcc
     7141 gatatggcaa actgaagcga ttcgcccagg tcggtttcgg ttttcttctt taagtcatat
     7201 accctcgcca cctgcgaacc cctgcccgaa ccgtagtagc tgtagtaaac cttcccgtca
     7261 atacatgcgg caactctgta gtttgcagca tcaataggga gtgatacggt tcggtcaact
     7321 atcccgtcta tatcaatttg aacaggtttt accgtgggct gtgcttcggt agccttttta
     7381 tcgtcggcat tttttttctt tgcatcggta accggttttg gctcaatctt cacctcgttg
     7441 ttttcgggag caaagggtga gggtgtgttc tttgagagca tgaccatgaa aattttgctc
     7501 atgtcggtgt aggcgtggtt ccattccgtg gagctatata ttgggttgaa gtccctgtcc
     7561 gaaacaaaaa ggaggtaaag cccatcgttg gagaagacag gattggttga ggtgtaccag
     7621 ttgcccgtaa cgttgtaggt tgaagccgtt tcggtgttat agaggaacac cacgctgaag
     7681 cgattttcat cctcggtgga gtaggctacc catttgctgt cgggcgacca cgagtagctg
     7741 tatattgagc catatttccc cttagcaagt tggctaactg ccttggtttc aacatcaacg
     7801 cactgcaacc ttgagagcct gtcggtgaaa agaatttttt tgctatcggg cgaccactca
     7861 atgttgaata tgtagttatt ggtgttggtt gtaagctgca ccggagccga ggctccatcc
     7921 tgtgcctgga gatagatttc gtactcaccg cttttatcgg agatgtaggc aatatacttt
     7981 ccgtcgggcg accaagctgc attgcggtcg tgggcgtcgg agctttgagt aaggttgcgg
     8041 gttattccgc tctcggcggg aagtgtgaac acatcacctc tggccgagaa taccacgcgc
     8101 tcgccgtttg gcgacaaatc catgctgcga atgctcttgc cggcatccac cagctcgctg
     8161 cggctgtaaa gctgatcgtc ggcaatagtc acgtcaatct tattggctgc cagggaactg
     8221 gcatcaaggg agtaaaggta tccccccttt tcaaaaacga tggtgttgcc tgagaatgac
     8281 ggaaatttga tatcgtagtc ggtaaagttg gttacctttt tggtctcttg ggttttgagg
     8341 tcgtagcaga acaggttcat ggttctgtcc ctgtcggaga ggaagaaaat tttaccgcca
     8401 atccacatgg ggaaaatctc ctgggtgttg gtgttggtga tgttggtgat ttgcttggta
     8461 tcgaaatcaa aaattcggat atcgtcggcc attccacctt tgtagtactt ccatgttcgg
     8521 aattcgcgaa aaacccagtt aaaggcaagc tgttttccgt cgggcgagta ggagcagaat
     8581 ccccctccgg tgaggggtag ctcactggac aaaccgccat caatgggaac catgaatagc
     8641 gaaccgacaa aatcgttgta ggattgtttt cgggaacggt acacaatgtt tttaccgtcg
     8701 ggtgtccatg tcattacgat gttgttgggg cccatacggt ctgaaatgtc atcacgcgaa
     8761 agggttgccg tataagtaag ccttttaggt gttccacctt cggcaggaat ggtaaatacc
     8821 tcggtgttac catcgtactg cccggtgaaa gcaattgttt ttccatcggg cgagaaacgt
     8881 gcaaacatct cgtagccaat gtggctggtt agcttgcgag ccacaccgcc attgagtggc
     8941 acagtatata aatcgccagc ataggtgaac accacctgat ttccgtggac tgccggaaaa
     9001 cgcattaacc tggcctcctg ggcacctgct aatccaacgg acagcaaacc caaaagaagc
     9061 atcatgctgc aaactttttt catcttgaaa ttattttagt tgaagttatg gttgtgttcc
     9121 tgtaattact catgttcgat aggggaatga ttgtttggtt taatcagctc atctcaaaac
     9181 cttgatagta tgactaacta acgtcgggca gtgcgaaacc tttaaattta gaaatgtaaa
     9241 tctctcagca ggaattactt tacaagtacc taaaattttt gcagatttca aaaccctaaa
     9301 gcaaaatgtt ttcatgcact gcttgtccct gtgaaaatga cgtaaaacct gctacttttg
     9361 ttgcatcata aatactaggt gatgaggcat atcggttatt ttcttttagc agcaaccgca
     9421 gcgttggcat tgccgagcag tagtaatgcc gttggtttga aaaattgcca ttcaggcaat
     9481 gggtctgcag aaaaaaaaat acaggagaca tgggctgatt cggttttgtc atcgcttacg
     9541 ctgagggagc gaataggcca gctatttatg gtggctgcat actccaaccg cgacgacatg
     9601 catgttaggg aaattgccga tttggttagg aaggatggag ttggcgggtt gatttttttt
     9661 cagggagggc cggtaaggca ggccaatctt accaactact accagtcatt agccgaggtg
     9721 ccgctacttg tgggcatgga tgctgaatgg gggcttggca tgcggctcga tagcaccttc
     9781 tcctatcccc gccagatgat gcttggggct gctcaggatt ccgctttggt ttacgaaatg
     9841 gctgccgata ttgcccggca gctgaaacga atgggtgttc acatcaactt tgcaccggtt
     9901 gttgatatca acagcaatcc acaaaatccg gttatcaata cccgttcgtt tggagaaaac
     9961 gttaacctgg ttagccgcta tggcaaagcc tacgtgtccg gattacagga taatggggtt
    10021 atggcatgtg ccaagcattt ccctgggcat ggagatacct ttgaagattc acacttttcc
    10081 ctgccaaagg ttcctcacag caagcagcag ctcgactcag ttgaacttta cccattcaaa
    10141 cagcttatca gctacggagt acaaagcgtt atggttgggc acctaagggt acccgctatg
    10201 gagaatgaca ccttactggc ctccagccta tcacctgcca ttatttccgg taaccttatt
    10261 tccgaaatgg gatttaaggg gctgatcttc accgacgccc tgaacatgaa gggggtagcc
    10321 gattacttta gccctgagga tattaacctg aaagccctgc tggccggtaa cgatgtgctg
    10381 ctttttccat ccgaggtgag ccggtcaatt gataaaattg agaagatggt gcgtagagga
    10441 cagatacccg aggaagtcat caacatcaaa tgccgaaaag tccttatggc aaagtacgct
    10501 gcgggactta gcagctacaa acccattgag gtactaaatc tttacagcga tctcaacagg
    10561 gtgtcctcgg aggttatacg taggaaaatc ataagccgtg cgctaacggt ggttcataat
    10621 aacaatagct tcatccctgt aaaaaggctc gatacgctca ggattgccta cctggagata
    10681 gggtgtaacc ggggtgatgc atttagggaa cagctggagc tttacaccgc aattcatacc
    10741 tttagtatcg atccttcttc gcctgccgaa gagttcgatt cgctgctgaa catactcgag
    10801 ccatacaacc tggtaatagc cggctaccat gctactgata gccgattttc tatgaactac
    10861 ggagtaactt gccaggctgc taattttctc ttcgatttgg ccttcagcag cgcagttgtc
    10921 ctcgacatat ttggaagccc ctatgccatt tcaaaattct ataatcccga ctcatttcgt
    10981 gccattatag tttcgtatga caactcgttt gatgctcaga atctttcagc ccagttgatt
    11041 tttggaggcc ggtttgctac tgggaggctg cctgttaccg caagtgcctg gtattctgcc
    11101 ggatatggag taacaactat caaccccatt cgcttaggtt acgccttgcc tgaggaggta
    11161 ggcataaact cactgctgct taaggaggtt gactccttgg ccaattcagc tatcgagctt
    11221 gctgttgccc cgggaatgca aattctggca gctaaggatg gcgtggtgtt ctaccataaa
    11281 agttttggaa agcccaccta tctttccagc gattcggttg atgttcgtat gctctacgat
    11341 gtggcatcgg taaccaaggt ggtggcaaca gttccatcgg tcatgaggct ctacgacgag
    11401 caggcacttg aacttgataa taacctcgga gcctacctcg atttaaagga attccccgac
    11461 aagtcagcaa taaggctatc cgacctgctc aggcatcaat cgggacttaa atcatggata
    11521 cctttcaacc tgaaaaccct taccacaatg attcccaata taccactatg gcaggagaag
    11581 cttagcaccg attatccgtt tcagcttggg agcaagcaat ttttcagccg ctacacagtg
    11641 ccaaatccgg ttttttactg cgataaggaa tcgagggatt ttccaaaccg ctgtgcagaa
    11701 ggactatact ctgcctactt catggccgat accattttta gcgccattaa ccgctcagaa
    11761 ctctcccaac ccggcaagta cgtttatagc gacttagggt ttatgtacct gcagcgggtg
    11821 gttgagggca taacccagac gggtattgac cgatactgcg aggataaatt ctacagccgg
    11881 ttgggaatga gctacacgct cttcaagcct ttaacccgtt ttgaccggga cagaattgtt
    11941 ccaacggagg tggatttgat tttccgacgt cagctggtat ggggcgatgt gcacgatccg
    12001 gcagcagcca tgctgggcgg ggtatcggga catgccggca tcttctccac tgccaatgac
    12061 ctggctaagc taatgcagat gttcctgcag gacggaactt atgggggtga acgctttttc
    12121 cgtccgggca ctgtggagca attcaccaca gccccaattg aggccaacag cagcgataat
    12181 agacgctctc ttggatttga caaacccgca tcagagggtc catcaccagc cggaagtttg
    12241 gcctcttcac aaagttttgg acacaccgga tttaccggaa caaccgtctg ggctgatccg
    12301 gagaatgggt tggtttatat tttcctttcg aacagggttt accccgatgc ttcgaatacc
    12361 aagttggccg aaatgaatat ccgcaccaga attcacgatg ttttttacag ggctattggg
    12421 gtagcaaaat gaggggtagc caactcacaa tctgaaaaat gaaagattgc gaagcatggt
    12481 gtacgctcaa agtagcccct taaattatct gttctttatg gaataataaa tgactaaaaa
    12541 gaaaagacgc ctgtttaagc gtcttttctc catttcaagt actagctgtt gctatttttg
    12601 tgcctcttcg gatgtcataa caacctcaac cacattgttt tggttgaata gcttctgtgc
    12661 aaagtccttt agactctcca cgctggttga tttcaccacg ttgaggtatt ccgtattaaa
    12721 atcaacgcca tcctcatact tcatcctaat ggcatcggac cagtagctgt tttctctttg
    12781 gttttgctcg tactccttca tcaggaattc ctttgtttta ttaaggtcgt cgggagtagg
    12841 gccattctgc ttaatctttt caatttcggc atacacaatt ccaatgagct tatcgcgaag
    12901 cttgggatcg gtgtcaaact ggatgccggc agtaaatgac tgggttggaa agttatcaat
    12961 gctgatattt acgcctacgc cgtaggtgcc gccttccttt tcacgaattt cttcaaggta
    13021 gcgtgtgcga agaatagagg taaggtattc ggccatcaca tggtttttga ggttatactc
    13081 ggcattacca gtaaatccta caaacactga tgatttagca gtttgcatgg gtttggtgaa
    13141 gtagttttgc acctttccct tgggcggtct aacaccgttg tccctgggaa tatcctttcg
    13201 cttaactaca ggtagtgcac caagatactt agctgtgagc gctttagctt catccacgtt
    13261 gatgttgccg gtaaaaataa aggtgaaatc ggaagcatcg gcaaaccggt cgcggtagaa
    13321 gctgtagcac ttgttgaaat caaccttact cagaaaatca gtatttatcg gcataaccct
    13381 agggttatgg ttagccatgg cagtgttaac actatcacgg aaagccattc tgggatcggc
    13441 agcggcattt gcaaaaaatg cctttagcct gtctaaatac gtgttgaagt tctcctggtc
    13501 ctgacgggga gccgtcatgt aaaggtaaac cagctggagc atggtttcaa aatcctttgg
    13561 agatgcgctt ccctgaaatc cctcgaaatc ctctccaaat acagggttaa cacgaaccat
    13621 ttttcctgct agcagctttt cgaggtcaac gctcgaaaac tcacccacgc cagcatttac
    13681 aaccatatcg gtagccaggg tagcagatgg tagcgttgct aaatcggcca gcgacaagcc
    13741 tcccttgctg aaggcatcca gcttaacctc gtcttctttg aagtcggtag gtttaagaat
    13801 tactttaact ccgttggaga gcgtaagctc cgtggtgcca aagatgtttt tgtcgtttac
    13861 cttctttatt tgacctggag taggaatgct ttcaaccaga ggtttcatca ccacgttatc
    13921 aacgtatgcc tgcaacgtct cggattttac ctctttaaca gcagccagaa tggcctcctc
    13981 gcttggcagc tgaatatcat cctttttcgg tgccataagg gttaacgcaa cattcttatc
    14041 ggtaaccata ctggcaatgt aggcgttgat ctcttcaagg gtaatggtag gaacaagccc
    14101 gtttgcaagc atgaattcaa attcaatgcc cggcatgggc tccccttcca gaaagttgtt
    14161 aatagcctcc caaacatact tctgattctt ctgcttatcc ttttccttca gctcgttttc
    14221 taacccgcgc atgtagtccg acttagccct gtcgaactcg gtagccgtga aaccaaactg
    14281 cttaacccgt tcagcctcgc gtattaatcc cttaatgcca tcaatcatgg cgtcgttacg
    14341 tagagcagca tagaacatca gggcgctttt ggtccgaacc atgttcccca tatcggaagc
    14401 ggcaaaaaca aagggagggt taggtttctg ggtaatttca ttcagccggg cagaaatcat
    14461 ggaagttgcc agcttttgga tcatttgctc ccgaacgtag ccaagatttt tttcgttttt
    14521 gggaactgcc tgatgcttgt agtaaacggt gaggatggtg ttgcgggctt cggggtcgga
    14581 cgcaatgccg attaacggct cgtcgttatc gggtaactcg aagaaaggtc taacgtccgg
    14641 attctcccgt ttgggtatgt cggcaaacag cgttttgatt ttctgctcaa cctggtcaac
    14701 atcaacatcg ccaacaatga cgatggattg taaatcggga cggtaccatt tgtggtagta
    14761 gtttttaatt tcctcatgct taaagttatt gatgacgttg atatcgccaa taacatcgcg
    14821 ctcggcgtat ttcgatccct tgtaaatcac cggcatcacc tgtttataca tccttcgttc
    14881 ggcattacgt ccagtgcgcc actcctcgcg gataactcca cgttcagctt caatttcatc
    14941 gggttcaagc gagatgaatc ctgaccagtc gtgcagcacc agaagggcgc tgtcaattat
    15001 tccctcgcgg ttggtaggaa catcggacag gtagtaaacg gtttcgtcaa gcgaggtaaa
    15061 ggcgttgatg ttgaagccaa acttaacgcc aaccgtttca aagtagttga ttataccctt
    15121 acccggaaaa tgcttggtgc cgttgaaagc catgtgctcc agaaaatgtg ccagaccgtt
    15181 ctggttatcc tcctcaagta ttgcacctac attctgggca ataaaaaatt ctgcgcgctg
    15241 ttgaggttcg gtgttcttcc taatgtagta ggttaggccg ttctccagct ggccaaaccg
    15301 aacattcctg tcaagaggaa tgggggtgtt ccaattcacc tgatcctgag ccccagcgct
    15361 aaaacctagt agaataccca gggcgcatag ataaaatcgt gtttttttca ttacccgatg
    15421 tttaagttaa ttttttgtta ttagtaaaaa tttatatcaa aggtagccaa attttatgtt
    15481 acataaacta aacgttcact ttttaacata ttttgaaact ttatagctgg attaagccct
    15541 ggctcaataa gtatttttct aaagtgttca ttttgcccgg tttctctggc ctggtttata
    15601 tatgcaaaat cccaacaatc aggattatcc ttatgctttc caatggactt ttatcttgta
    15661 tttgccaacc tgttcaaatg cctgtttcct atgccaacaa ttaaaatttc tgcagctgaa
    15721 tatcaaggca acatttgttt gaataacttt ccgggctgtt cggaaaggat ggcaacatgg
    15781 ttaaataatt gaattattgt tctttatttt tttattcata gtaagttctg aggtttaact
    15841 agctgctaat tattctttgg atatttaatt taacctactc tgcatgaaaa agtttctaac
    15901 ccgtaccatc gtgatgcttt cgctggtaag cctgtttacg gatatagcca gcgaaatgct
    15961 ttacccaata atgccggttt ttctcaagtc cattgggttt tcggttttgc tgattggtat
    16021 tcttgaaggg ttggctgagg ccacagctgg aataagcaag ggctacttcg ggcatctttc
    16081 cgacaggttg gggacgcgag taccctttat aaggtggggc tacaccctta gcgccatctc
    16141 caagcccctg atggccgttt tcacctggcc gatttggata tttggtgctc gaacactcga
    16201 caggcttgga aagggcatac gaaccagcgc ccgcgacgcc ctgctcagcg acgagtccac
    16261 cagggaaaca aaggggaagg tatttgggtt tcatcggggc atggatactc ttggagcagc
    16321 tattgggcct attgctgcgc ttatatacct gcacttctac ccccaaaact acagcacgct
    16381 tttcctgata gcctttgctc caggtctggt ggcagtttcg cttaccctct tactaaagga
    16441 taagaagaaa ccagaaacaa gtaaccaaca ggttaatcct gttcagggta agggattttt
    16501 ttcttttgta aagtactggg ggcattctcc caaagctttt cgctacctgg tggtaggcct
    16561 gctgtttttt acccttttca acagctccga tgcctttctg ctcctcatgg ttaaggagaa
    16621 agggttctct gatgccaaca tgatttcgct ctacatattc tacaacctgc tctatgcgct
    16681 gcttgcctac cccatgggtt ggctggccga taagtttggc atgaagcgga taatgatctt
    16741 tggcgtatcg gtgttcggcc tggtttacct gctctttgga tttgcagaca agctacacat
    16801 gttcctgctg ctctttggat tatatgcatt ctatgcagcc gcaaccgaag gcgtttcaaa
    16861 ggcttggata actaacattg tgcctggcga aagcacggca accgctatag gattctacac
    16921 cggattccag agcgtggcaa ctcttttagc cagcagtatg gccgggttaa tctggactaa
    16981 gtttggagca tcaactgttt tcgttatttc gggcttagcg gcattcataa ccgccatcta
    17041 cctgatgctg gcaaccagaa tgaataagtt agcgtagccg gtcaagcgaa cgtataaggg
    17101 cttcgtcctt agcaattgcc cggtaggcaa ggtaggtgaa aatggcgcaa accaagggga
    17161 aaatgaatat caagccatac gatgggggtg ccattagcct ttgctccaca gctttagcaa
    17221 tataaaatag caagccctgc aagccaatca gaagcacgat gttgaaaatg cacaaccttg
    17281 cctgcagttt tcttttttta aagaggaata tggttaccag ggtagcggta aaagccagta
    17341 tgaccaaagc aaggactgcc caaagtttag gtaggttctc cactactgaa ccatcctgac
    17401 ctaaacccga tgcgctaagc gaaattttaa cctcctgtgg cccaacagcg taatgggcgt
    17461 atggcatgaa aaagaaaaat gccagtaaaa ttgcagatat taggaggtaa agcgattgta
    17521 ttctctgtat catggctgaa tatgcttaaa agtagtttta gacaaatgta tgaaaaaatt
    17581 acctcctcag aacgaaggaa aggataaagg atttca
//
LOCUS       DAOPHX010000042        17226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_3722_length_17226_cov_6.018753, whole genome shotgun sequence.
ACCESSION   DAOPHX010000042 DAOPHX010000000
VERSION     DAOPHX010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 17226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17226
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3722_length_17226_cov_6.018753"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PK502_04720"
     CDS             <1..190
                     /locus_tag="PK502_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00154.1"
                     /translation="LPFFIAGVRTEERQRRARERSGQSLSLRTGQKLGIGAFAFFCFG
                     VRTEERQRAIPSPSTRNG"
     gene            complement(300..2117)
                     /locus_tag="PK502_04725"
     CDS             complement(300..2117)
                     /locus_tag="PK502_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPH00155.1"
                     /translation="MKKFFSLLKFIIPYKWHVVANVVGNILGAIFSLFSLAMLIPFLQ
                     LLFGTVPLVEVKPEFTMTIKSLNDTLNYMLSEMIRNHDRSTAMLFVIGLVLTASFLKN
                     FFTYMSLYYLAPIRTGVLRDIRNTLYRKVVDLPLGYFTEEKKGDVISKMTNDVNEIEV
                     SIIRSLEMIFRDPIIIIIHLIGLIYISPQLTLFVLLLLPLTGVVIGRVGKTLKKTSFK
                     GQRKMGAILTLIEETLSGLRVVKAFNAEKKVISRFESVNNFYTLLMKKMWRRRDLASP
                     LSEFLGTIVVVLVLWFGGNLIFKGKGTLTPEALIAYIGIFYMIINPAKSFSNAYYNVL
                     KGMASADRIDTILSAENPIKIKPEAKVLTEFSSAIEFRNVNFRYQSEPVLHNINLVVE
                     KGKTVALVGQSGSGKSTLVDLLPRFWDVTEGEVLVDGVNVKDCNLESLRNLMGNVNQD
                     PILFNDTFYNNIAFGMPSATEEDVIAAAKIANAHDFIMATPNGYYTNIGDRGSKLSGG
                     QRQRISIARAILKNPPIMILDEATSALDTESEKLVQDAIENLMKHRTSLVIAHRLSTI
                     RNADMICVLHEGEIVERGRHEELLRLNGYYAKLHNMQLK"
     gene            complement(2182..>3396)
                     /gene="purB"
                     /locus_tag="PK502_04730"
     CDS             complement(2182..>3396)
                     /gene="purB"
                     /locus_tag="PK502_04730"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPH00156.1"
                     /translation="IALCNLPLPQLKKFRTGNFDKLRGIYEKFAEGDAARIKNIESTT
                     NHDVKAVEYFLREKFDELGFSDYKEFIHFGLTSQDINNTAIPASIRDFMNSCYYPTLS
                     KLLEKLHDLSNEWAEIPMLAHTHGQPASPTRLGKEIRVFAERIEVQLLQLRSIPYSAK
                     FGGATGNLNAHLVAYPEVDWVAFANGFVENTLGLKRSQTTTQIEHYDNLAALFDCLRR
                     INTILIDLCRDFWAYISMEYFKQRIVEGEVGSSAMPHKVNPIDFENAEGNLGLANAIL
                     DHMATKLPISRLQRDLSDSTVLRNVGVPFAHIQIAIQSIVKGLNKLIINNEVIKNDLE
                     TNWMVVAEAIQTILRREGYPNPYEALKKLTRNNKNVTQKDFLTFIDELDIDSRVKTEL
                     KSITPFNYLGVK"
     assembly_gap    3397..3406
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3407..3580)
                     /locus_tag="PK502_04735"
     CDS             complement(<3407..3580)
                     /locus_tag="PK502_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPH00157.1"
                     /translation="MSLNALHAISPIDGRYYSKTKELQNYFSEFALIKYRVWVEVEYF
                     IALTYALPELAGFS"
     gene            3692..4450
                     /locus_tag="PK502_04740"
     CDS             3692..4450
                     /locus_tag="PK502_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HPH00158.1"
                     /translation="MEKLVKISAISYLNTAPFAYGLRHSDIFSKIQLLSDYPSECARK
                     LQYNEVDISLLPVGALPSLSHYEVITDFCIGAIADVRTVVLLSNSPLHRIRKIYLDYQ
                     SRTSVQLVRILSRYYWNINPEWVPLSPEQDYSTIPKDYGIVLIGDRVFEVESRYQFVY
                     DLSGEWGKFTGLPFVFAVWAAASQPTDDFKHQLNHALQLGVNSIPQSVDEFALKNIER
                     DEAIRYLNQNISYPLDAEKRKAISLFLSYMSQNQ"
     gene            4532..5962
                     /locus_tag="PK502_04745"
     CDS             4532..5962
                     /locus_tag="PK502_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPH00159.1"
                     /translation="MKRLKDVASRLACGIVAVFLLTIPFHLVYAGEPATNSPTVVYKI
                     DIQENIMPAAWRHVKRGFEEAARINADIILIHLNTYGGMVDMADSIRTKILNSKIPVW
                     VFIDNQAASAGALISIACDSIYMRKGGSIGAATVVDQSGNVVPDKFQSFMRGMMRATA
                     EAKGKDTIVTKGDTLLKWRRDPRIAEAMVDPSIFIEGIIDSGKVLTFTADEAILHGYC
                     EGKAETVDEVLAKAGVESYRLEEFTPTFIDRIIGFLINPVVSGILIMLIIGGIYFELQ
                     SPGIGFPLVVAVLAAILYFAPLYLEGLAQHWELALFVVGLILLFVEIFAIPGFGVTGI
                     SGIILMVVSLTLAMIDNNLFRGVGSFNVLILLKPLAIVLVAGFLGFLGGLYLSKKFLT
                     SSMFSNLALNSSLSKEDGFVGVDKQIKTKIGLEGIAATMLRPSGKVKIGDDWFDAISE
                     YGYINKGERVRVTRDEAGQLYVVKAE"
     gene            5976..6419
                     /locus_tag="PK502_04750"
     CDS             5976..6419
                     /locus_tag="PK502_04750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPH00160.1"
                     /translation="MENFSVRDFLPSDFEALSLVWSLTGLGNPARGDNLEIIMQSIAM
                     GGKLLVATSSQGLVVGTSWMTFDGRRIHLHHFGVHPSYQRRGIGKMLAAESLRFVKQK
                     GYQVKLEVHQGNLAAINLYKQFGFQRLGDYDIYIIRDLTVIDSVL"
     gene            complement(6453..7877)
                     /locus_tag="PK502_04755"
     CDS             complement(6453..7877)
                     /locus_tag="PK502_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPH00161.1"
                     /translation="MLKIQIQEDIKSYLGYTPTSGQAAAIDILSDFTLNPDSGKCLLI
                     KGYAGTGKTSLIAGYVKTLQKLKVPFELLAPTGRAAKVLTDYSGIKALTIHKCIYRQK
                     SISEGIGKFVLNLNKLTNAIFIVDEASMISNSSFEDSAFGSGKLLSDMVEFVKQGKNC
                     RLIVVGDTAQLPPVGLDISPALDARELAMFNLDVQEIFLNEVVRQDLGSGILFNATRI
                     RQILDSTAISTPKFDVSTRFKDIERIDGGSIMEYLTEEYSRSNRNSNVVICYSNKQAN
                     RYNIAIRSRILFNEEELSVGDYLMVARNNYYWVADNPNIAFIANGEIVEVKRIGKHYN
                     LYGFRFADVTLKLTDEDNQEVDARIILDSLTIDGPSLGSEQMKNLYAAVAQDYQHIKT
                     YRTRLQKIREDPFFNAIQVKFAYAVTCHKAQGGQWPNVFIDQGFFRESMINREYLRWL
                     YTAVTRATSKVYMVNFNDIFFGNE"
     gene            8105..8941
                     /locus_tag="PK502_04760"
     CDS             8105..8941
                     /locus_tag="PK502_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HPH00162.1"
                     /translation="MLKPLKLIDESFDRDRTGSYDLSVTISINGFSFAVLDTLRNTFI
                     VLVSTPKSEQQDFEELFNQAMVDYPWLAGSFKHVYLSFFSPYFTPVPGKFFSEQKARQ
                     LFELVHPLPDGFELHFTPNCNHTDATLLFALPNSVANWWLKLHPKASFLHPIAPLLSS
                     PAAGSNVLLSVDFNENDFLVALHDGMKFAGANAYTGRHPNDVIYYLLAFCKELGYDSR
                     NVKVSITGHSPMLAELNMLDGYFDRVSSIAQYPKVMLFSYQLISHRSEYFSLFNLGAS
                     CE"
     gene            8932..9471
                     /locus_tag="PK502_04765"
     CDS             8932..9471
                     /locus_tag="PK502_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HPH00163.1"
                     /translation="MRIVGGSLRGRRFDPPKNFGARPTTDFAKEGLFDVLSNYFDFEE
                     LTVLDLFSGTGSISLEFASRGARHIDAVEKIYAHQKFIKDSALRLGVTSISVIRQDVF
                     SFISRSTCRYGLIFADPPYDMEGISTLPELILSKDLLDEDGWLVVEHSDRYSFSGLKQ
                     LIDHRRYGSVNFSFFRKNI"
     gene            9532..9606
                     /locus_tag="PK502_04770"
     tRNA            9532..9606
                     /locus_tag="PK502_04770"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9567..9569,aa:Asp,seq:gtc)
     gene            complement(9691..10530)
                     /locus_tag="PK502_04775"
     CDS             complement(9691..10530)
                     /locus_tag="PK502_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPH00164.1"
                     /translation="MAYNLLKGKKGLIFGALNEKSIAWKVAERAHEEGAEIVLSNTAV
                     AMRMGSIDELAAKCNAIIIPADATSIPDLENLTDKTMEHFGGKFDFILHSIGMSPNVR
                     KGRTYDDLDYDYLQKTLDISAISFHKIIQTCWKKDAITHGGSIVALSYIAAQRTLYGY
                     NDMADAKALLESIARSFGYIYGRERGVRINTVSQSPTETTAGSGVMGFDALLDFSNRM
                     SPIGNANADDCADFCITLFSDLTRKITMQNIYNDGGFSSMGMSNKAMAIYNRNLEECK
                     DCN"
     gene            complement(10591..12261)
                     /gene="recN"
                     /locus_tag="PK502_04780"
     CDS             complement(10591..12261)
                     /gene="recN"
                     /locus_tag="PK502_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPH00165.1"
                     /translation="MLRKLYIQNYAIIDELTIEFQPGLNIITGETGAGKSILLGALML
                     ILGQRVDASILKDQSKSCIVEGEFDISSYDLDDFFRVNDLDYNPTTIVRRQIGESGKS
                     RAFINEIPVNLTLLKELGDRLIDIHSQHQNLLLGTSRFQLNVVDTYAIPKNLQSAYLE
                     TYLNYRQLEAELKKLEDEAANFGRDFDYLQHQLEELRAANLKEGELEELEIEQAKLTH
                     ATEIKSALEFTINAASNDETSAIALLKEASASLKKISPYYPAADELEHRLESCRIELR
                     DIVDEAERRSSQVNVDDELLASVSQRIDLLYTLLQKHRKGTMTELIAYRNSIEERVNH
                     ISGMEFNLETRRKDLERRGHELRKKADELSILRRKAAAPIEQSITELLIQLGIKHAIF
                     KVDIQPVNEFQPWGTDRVTFYFSANSQIPPMELSKIASGGELSRVMLSLKSLLVKASG
                     LPTIIFDEIDTGVSGEIADKMGSIIHTMAKNMQVINITHLPQIAAKGKTHFLVYKENQ
                     ELRSTTGIKLLTPEERVVEIAKMLSGEKMSDAAILNARELLKSNGSLN"
     gene            complement(12280..13203)
                     /locus_tag="PK502_04785"
     CDS             complement(12280..13203)
                     /locus_tag="PK502_04785"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HPH00166.1"
                     /translation="MHKSFAKLMLTLLLIAPLVLDGQELRCNVQINSQKIQGTNRTIF
                     QTLQTAIYEFMNNTAWTNHVYGFDERIECSIMLNITEQLGSDEFKGTLQVQSRRPVFN
                     SSYNTTMLNFQDNNLHFRYREFDKIEFSENSHLSNLSSILAFYAYIILGIDYDSFSML
                     GGTDYLLKAEKVVNNAQNAPERGWKAYEGNRKNRYWMIENLLNSKYRPLREVLYRYHR
                     LGLDRMSDKAVEGRAEIAECMATLQKVYREKPDPYLFALQLFFDAKSDELVNIFSESF
                     PSEKARVVNILTEIDNANAGKYQKILEQKNS"
     gene            complement(13225..14436)
                     /gene="coaBC"
                     /locus_tag="PK502_04790"
     CDS             complement(13225..14436)
                     /gene="coaBC"
                     /locus_tag="PK502_04790"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPH00167.1"
                     /translation="MRLAGKHIILGVTGSIAAYKAAILTRLLIKEGAEVKVIMTPHAK
                     EFITPLTLATLSKNPILVDFFNPENGGWNSHVDLGLWADLFLIAPASANTMAKMASGV
                     ADNLLLTTYLSARCPVMLAPAMDMDMYSHPATQKNIQILKSYGNIFVEAATGELASGL
                     SGKGRMEEPENILQHVVNLFLAANRFNGYKVLITSGPTYEPIDPVRFIGNYSSGKMGT
                     AIATAIAQQGAQVTFITGPAAVEPQHPSVKIVRVKTASEMLEQAKNEFDSCQVAILAA
                     AVADFAPANTSTDKIKRGKENLDLQLEPTTDIAETLGKMKRQNQIIVGFALETHNEVE
                     NAIDKLTRKNLDIIVLNSLNDAGAGFGHNTNKITLIDRNGKQKSFELKPKSEVAQDIA
                     NAIIDLLQQAK"
     gene            complement(14436..14771)
                     /locus_tag="PK502_04795"
     CDS             complement(14436..14771)
                     /locus_tag="PK502_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004349573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPH00168.1"
                     /translation="MDLKKMNVPASTITRNLDELDKETGNIYQTVRIIAKRANKLSVD
                     IKQELNHKLEEFAYYTDNLEEVFENREQIEISKYYEKLPKPTLIAIQEFMDGKLYYKM
                     VDNTEVTNQ"
     gene            complement(14789..15601)
                     /gene="bamD"
                     /locus_tag="PK502_04800"
     CDS             complement(14789..15601)
                     /gene="bamD"
                     /locus_tag="PK502_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPH00169.1"
                     /translation="MTKHRDTKGILFLFTILATIFMVSCSGYEKLLKSKDYEYKYKKA
                     LEYYEKKEHYRYTTLLEQLSPIYKGTFRSDTIEYYIAQGYYYQGDYLLAGHSYDKFRQ
                     TFQRSAFTEDAEFMYAYCFYKSSPRPELDQASTQEAIEAFVEFLTRFPSTNRRTEVNG
                     LLTELREKLVEKSYLSSKLYYKIGDYKAAIVAIQNSLKDFPNSKYREELSYLLVKSSY
                     LLAENSIPNKQRERYQNTIDQYYTFVGEFPESPYLPEAKKMYESSMKVLQIN"
     gene            16201..>17226
                     /locus_tag="PK502_04805"
     CDS             16201..>17226
                     /locus_tag="PK502_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPH00170.1"
                     /translation="MDIFDKIDNMGPIGQWQSQSHGYFSFPKLEGEIEPHMIFRGKRV
                     LNWSLNNYLGLANLPEVRKADAEAAAQYGLAYPMGARMMSGQTRKHEELEEELARFVG
                     KPSAYVLNYGYQGMVSIIDTLLGRNDVVIYDSESHACIIDGLRIFPGKRFVYQHNDME
                     SCRKQLEHATAVVEKTGGGILLVTEGVFGMAGDLGNLKAITDLKKEFNFRILVDDAHG
                     FGTMGATGAGTSEHFGVQDQVDIYFATFAKSMAGIGAFVASQEHVIRFLAYNMRSQIF
                     AKSLPMAMVLGIQKRLEILKSHPELRENLWNIAGELQKGLREIGFDLGRTQSPVTPVY
                     MFGGIPEA"
BASE COUNT         4265 a         3712 c         4100 g         5139 t
ORIGIN      
        1 tttgcctttt ttcattgcgg gggttcgaac cgaggagcgc cagcgacgag ctcgcgagcg
       61 gagcgggcaa tccctctctc tccgcacagg gcaaaagtta ggaattgggg cttttgcctt
      121 tttttgtttc ggggttcgaa ccgaggagcg ccagcgagca attccttctc cctccacaag
      181 aaatggttga gttttttagc cctttgctgt ttgggagaag ttgcctgtaa aaaataggaa
      241 cgatgcttca ggaaatttca gtggcaatat ttacttgcag cttgtgtcgt ctgaaggtgc
      301 tattttagct gcatgttgtg aagcttggcg tagtagccgt ttagccttag cagttcttcg
      361 tgccttcctc tttcaactat ttcaccctcg tgtagaacgc atatcatatc ggcatttcgg
      421 atggttgaaa gtctgtgggc aataaccagt gaggttctgt gcttcattag gttctcaatg
      481 gcatcctgaa ccagcttctc cgattcggta tcaagggcgc tagtagcttc gtcaagaatc
      541 attatgggtg ggtttttaag aatggcccgg gcaatgctga ttcgctgacg ttggcctccc
      601 gagagcttgc taccccgatc gccaatgttg gtgtagtatc catttggtgt agccatgatg
      661 aagtcgtggg cgttggcaat ttttgctgca gcaatgacgt cctcctcggt tgccgagggc
      721 attccaaagg caatgttgtt gtaaaaggtg tcattgaaga gaattgggtc ctggtttaca
      781 tttcccataa ggtttcggag gctttcgaga ttgcaatcct taacatttac cccgtcaact
      841 agcacttcac cttcggttac atcccagaaa cgtggcagca ggtccaccag ggtgctttta
      901 ccagaacctg actgccctac cagagccacg gttttcccct tttcaactac aaggttgata
      961 ttatggagca caggttcact ttggtacctg aagttgacat tacgaaactc tatggccgag
     1021 gagaactctg tcagcacttt agcctcgggt tttatcttaa ttggattctc ggccgaaaga
     1081 atagtatcaa tgcggtcggc agaggccatt cccttcagca cgttgtagta agcattggag
     1141 aacgacttgg cggggttgat aatcatgtag aatatgccaa tgtaggctat tagcgcctcg
     1201 ggggttagcg tcccttttcc cttaaaaatc agatttcccc caaaccatag caccaacaca
     1261 actacgattg ttcccaagaa ctcgcttagg ggtgaagcca gatcccttct gcgccacatt
     1321 tttttcatca acagggtgta gaagttattg acgctttcaa aacgggagat aacttttttt
     1381 tcagcattaa aggcttttac aacccttagt ccacttagcg tttcctcaat cagggtaagt
     1441 atggccccca ttttacgttg ccccttaaac gaagtctttt ttagggtttt gccaacccgg
     1501 ccaattacaa cccctgttag gggtagtagc agcagcacaa aaagcgttag ctgtgggctt
     1561 atgtagatta accctataag gtgaatgatg atgatgatag gatctctgaa tatcatttcg
     1621 agcgaacgaa tgatggaaac ctcaatttcg ttcacatcgt tggtcatttt gctgatgaca
     1681 tcaccctttt tctcctcggt gaagtatccc aatggcaggt caactacctt cctgtaaagt
     1741 gtgtttcgta tgtccctgag cacacctgtg cgaatgggtg ccaggtagta aagcgacatg
     1801 taggtgaaaa agttttttag gaacgaggcc gtaagcacca gtcctatgac gaatagcatg
     1861 gctgttgacc ggtcgtggtt gcgaatcatc tcgctaagca tgtagtttag ggtgtcgttc
     1921 agggatttaa tggtcatggt aaactccggt ttcacctcaa ccagcggaac agttccaaag
     1981 agcagctgca ggaacgggat gagcatggcc aatgagaaga gcgagaatat ggcacctaaa
     2041 atattaccta caacgttagc tacaacatgc catttatatg ggatgataaa tttgagtagg
     2101 ctgaaaaatt tcttcatttg agatttttgt gaaaaagcgt gtaaaggtaa gtttaatatt
     2161 tcagaaaatt tagaagaata cctacttcac tccgaggtag ttgaacgggg taatactttt
     2221 cagctcggtt ttaaccctac tatctatatc cagctcatca atgaaggtga ggaaatcctt
     2281 ttgcgtaacg tttttgttgt tgcgggtgag ctttttaaga gcctcatagg ggtttggata
     2341 tccttcgcgc cggagaatag tctgaatggc ttcggcaaca accatccagt tggtttccag
     2401 atcatttttg atgacttcgt tgttgataat cagcttgttt agccctttga caatggattg
     2461 aatggcaatc tgaatgtggg caaatggaac ccctacgttg cgcagaacgg tggaatcgga
     2521 aaggtcgcgc tgaagtctcg aaatgggcag cttggttgcc atgtgatcga gtatggcatt
     2581 tgccaagcct aaattgcctt cggcattttc aaaatcaatg gggttaacct tatggggcat
     2641 tgccgatgag cccacttcac cctcaacaat tctttgcttg aagtactcca ttgaaatata
     2701 agcccagaag tcacggcaca ggtcaatcag gatggtgtta attcggcgaa ggcagtcaaa
     2761 aagtgctgca aggttatcgt aatgctcaat ctgcgtggtg gtttgagagc gctttaaccc
     2821 caaagtattc tcgacaaagc cattggcaaa tgccacccaa tccacctcgg ggtaggctac
     2881 caaatgagcg tttaagttac cggtagcccc gccaaacttg gcggaatatg gaattgacct
     2941 gagctgcaat agctgaacct ctatccgctc agcgaagacc cgaatttcct ttcccaacct
     3001 ggtgggtgaa gcaggctgac cgtgtgtgtg cgctagcata ggaatttcag cccattcgtt
     3061 gctcaggtcg tgaagtttct ccaggagttt cgagagggtg gggtagtagc agctattcat
     3121 gaaatcacga attgatgccg gaatggccgt gttgtttata tcctgcgagg ttagcccaaa
     3181 gtgaataaac tccttatagt ctgaaaaccc cagctcatcg aatttttccc gtaaaaagta
     3241 ctcaaccgct ttgacatcgt ggttggttgt gctctcaata ttctttattc tggctgcatc
     3301 accctcagca aatttttcat atatacctct aagcttatcg aaatttccgg ttcgaaactt
     3361 tttgagttgt ggaagcggca agttgcaaag ggctatnnnn nnnnnncgaa aatccagcga
     3421 gttctggaag cgcataggta agggcaataa aatactccac ttcaacccac accctgtact
     3481 tgataagggc gaactccgaa aaatagttct gtagttcctt ggttttgctg tagtatcttc
     3541 catctatagg cgatatggcg tgtagggcat tgagcgacat aagtatagtt ttgaattttt
     3601 acaaaattac taaacattag ctattatgta gggtttccta caagttatat ttcagctacc
     3661 aatattttac taactttcgg aacttctgat tatggaaaaa ctggttaaaa tttcggccat
     3721 atcctacctt aataccgcac catttgcata cgggttaagg cattcggaca tattctctaa
     3781 aatacaactt ctttccgatt acccctcaga atgtgcccgt aagttacaat acaacgaggt
     3841 ggacatcagc cttttgcctg taggggctct accatctctt tcccattacg aggtaattac
     3901 cgatttttgc ataggagcga tagcggatgt gcggactgta gttttgttga gcaacagtcc
     3961 actccatcgt atcagaaaga tatatctgga ttaccaatcc cgaacatccg ttcagctggt
     4021 tagaattctt agcaggtact actggaatat aaatcccgag tgggttccgc tttctccgga
     4081 gcaggactac tctactattc ctaaagatta tggtattgtg cttattggcg acagggtttt
     4141 tgaggtggaa agcaggtatc agttcgttta cgacctttcg ggcgaatggg gcaaatttac
     4201 aggattaccc tttgtttttg ccgtttgggc tgcagcctcg caacccaccg acgatttcaa
     4261 gcatcagctt aaccatgcgc tacagcttgg ggttaattcc atccctcaat ccgttgatga
     4321 gtttgccctt aagaatattg aaagggatga ggccatccgc tacttaaatc aaaatatttc
     4381 ctaccctctt gacgctgaga agcgcaaggc catttccctc tttttaagtt atatgagcca
     4441 aaaccagtag tagtgctgca tagctatcag cgtcaatggc tatatttaca ctaaatttgg
     4501 agaagattcg ttaaagtaaa agtaccccaa aatgaaaaga ttaaaagacg tggctagtag
     4561 attggcatgc gggattgtcg ctgtgttcct gctaacaatt ccgtttcacc tggtttatgc
     4621 aggtgagccg gctactaata gtccaactgt ggtttataag atagatattc aggagaacat
     4681 catgcctgcc gcctggcggc atgtaaaaag gggttttgag gaggcagcca gaataaatgc
     4741 cgacattata ctgatacacc tcaacaccta tggcggcatg gtggatatgg ccgattccat
     4801 tcgtacaaaa attcttaaca gcaaaattcc cgtttgggtt tttattgaca accaagcggc
     4861 ctcggcagga gccctgattt caattgcctg cgatagtatt tacatgcgga aaggtggaag
     4921 catcggcgct gcaacggttg ttgaccagtc gggtaatgtg gttcccgaca agttccagtc
     4981 gttcatgcgg ggcatgatgc gggctactgc cgaagccaag ggtaaggaca ccatagtaac
     5041 caaaggcgac accttgttga aatggcggcg cgatccgaga attgcagagg ccatggttga
     5101 cccgtcaatt tttattgaag ggataattga ctccggtaag gttctcactt ttaccgccga
     5161 tgaagctata ctacatggct actgtgaggg taaggccgaa accgttgatg aagtattagc
     5221 caaagcaggt gttgaaagct acaggctgga ggagttcacc cccactttca ttgaccggat
     5281 tataggtttc cttatcaacc ccgttgtatc agggattctg ataatgctca ttattggtgg
     5341 tatatacttt gagctgcagt cgcccggaat aggttttccg ctagtagttg cagtgcttgc
     5401 cgccatcctg tactttgcac ctctttacct tgaaggcctt gcccagcact gggagctggc
     5461 actttttgtt gttgggctta tcctgctatt cgtagaaatt tttgccattc caggttttgg
     5521 agttaccgga atcagcggaa taattcttat ggtggtcagc ctcaccctgg ccatgattga
     5581 taacaacctg tttagaggag ttggctcatt caacgtctta atcctgttaa agccactggc
     5641 aattgtttta gttgctggtt ttctgggctt cttaggagga ctttacctga gcaaaaagtt
     5701 tcttacctcc tccatgtttt cgaaccttgc gttaaactct agcttgtcaa aagaggatgg
     5761 gtttgtggga gttgacaagc aaataaagac gaaaattggc cttgagggca ttgctgccac
     5821 catgttgcgt ccttccggta aggttaagat tggagacgat tggtttgatg ccatttcaga
     5881 gtatggttat atcaataaag gcgagcgggt tagggttaca cgtgatgaag ccgggcagct
     5941 ttacgtggtg aaagccgaat aattttgaat gtatcgtgga aaatttctca gtaagggatt
     6001 ttttaccaag cgattttgaa gcattatcgc tagtttggag cctaacgggt ttgggaaatc
     6061 ctgctcgtgg tgataacctg gaaattatca tgcagagcat agctatgggc gggaagctgc
     6121 tggttgctac cagctcccag ggccttgtgg tgggtactag ctggatgacc tttgacggtc
     6181 gtagaataca ccttcaccac ttcggcgtac atcccagcta ccagcgaagg gggattggca
     6241 agatgctggc agccgagtca ctaaggtttg tgaagcaaaa aggctaccag gtgaagctgg
     6301 aggttcatca gggtaacctg gctgcaatca acctttacaa gcagtttggc tttcaacgtc
     6361 tgggtgatta cgatatctac atcattagag atttaaccgt gattgatagt gtgctctagc
     6421 cttacccgtt agacttagca cagcagtgca agctactcat ttccaaagaa aatgtcgtta
     6481 aaatttacca tgtaaacctt tgaggtagca cgggtaacag ccgtgtagag ccagcggagg
     6541 tactcacggt tgatcatgct ctccctaaaa aatccctggt caataaacac gttgggccac
     6601 tgtccaccct gggccttatg gcatgtaact gcataggcaa acttaacctg aatagcgttg
     6661 aagaaaggat cctccctaat tttttgcagc cttgtccggt aggttttaat atgctggtaa
     6721 tcctgtgcta cggctgcgta aaggtttttc atctgctccg agccgagcga gggtccgtca
     6781 atggtgagtg agtcgagaat aatcctggca tctacctcct ggttatcctc gtcagtcagc
     6841 ttcagggtaa catcggcaaa acgaaagccg taaaggttgt aatgtttacc tatgcgttta
     6901 acctctacaa tttcaccgtt ggcaataaaa gcaatgtttg ggttatctgc cacccagtag
     6961 tagttgttac gggccaccat aaggtagtcg cctaccgaaa gttcctcctc gttgaacagt
     7021 atccgtgaac gtatggctat gttgtagcgg tttgcctgct tgttggagta acagatgacc
     7081 acgttgctgt ttctattgct acggctgtac tcctccgtca ggtattccat tatggagcct
     7141 ccatcaatcc tttcaatatc cttaaaacga gttgaaacat caaatttagg tgtagagatg
     7201 gccgttgagt ctaaaatctg ccttatgcgg gttgcgttga aaaggattcc tgaacctaaa
     7261 tcctgcctaa caacctcgtt tagaaaaatc tcctgaacat caaggttgaa cattgccagc
     7321 tctcttgcat ccagcgcagg gctaatgtcc aatcccacag gtggcagttg agccgtatcg
     7381 cccacaacta tcagcctgca gtttttcccc tgcttaacaa actccaccat gtcggaaaga
     7441 agcttccccg acccgaaggc gctatcctca aatgaggagt tggaaatcat tgatgcctca
     7501 tctacaatga aaatggcgtt agtaagcttg tttaagttga gcacaaattt gccgataccc
     7561 tcggatatac ttttctgccg gtatatgcat ttgtgaatgg ttagcgcctt gatgcccgaa
     7621 tagtcagtaa gaacctttgc tgcccttccg gttggggcta gcagctcgaa tggcaccttc
     7681 agtttttgca gagtctttac ataaccggct atcagggatg ttttacctgt tcccgcataa
     7741 cctttaataa ggagacattt cccagaatct gggtttaggg tgaagtccga aaggatgtct
     7801 atagcggctg cctgcccgga tgttggtgtg tatcctaaat aggacttgat atcttcctga
     7861 atttgaattt ttaacaccgc ggtactacat tttctgattt aaaaggttta atttggcttt
     7921 atgtttttaa atttgcgcaa acttatacta attacatctt tttgaaaaac ttttctttag
     7981 gaataatcat tcatgcaatc atcctttcat accaggaaag agagtatgaa gatttttttg
     8041 gttccgaaga agcatattaa tacacacctc tttggaggca ctttatcaaa caaggcaaat
     8101 aggcatgttg aaacccctga agcttataga cgaatcgttc gacagggata gaacgggcag
     8161 ctacgattta tccgttacca tttccataaa tggcttttca tttgctgttt tggataccct
     8221 acgaaatacc ttcattgttt tggtttcaac acccaaaagt gagcagcagg attttgagga
     8281 gctgttcaac caggccatgg tggattatcc gtggttggcc gggagcttca aacatgttta
     8341 cctttctttc ttctcaccat actttacgcc tgttccaggg aaattctttt ccgagcaaaa
     8401 agccaggcaa ctttttgagc tagtacatcc cttgcccgat ggtttcgagc tacatttcac
     8461 cccaaattgc aaccatactg atgccaccct actttttgcc cttcccaatt ctgtggccaa
     8521 ctggtggttg aagctacatc caaaggcatc atttcttcac ccaattgctc ccctgcttag
     8581 ttcacctgcc gcaggctcaa atgttttact aagtgttgat tttaacgaga atgattttct
     8641 tgtagctcta catgatggga tgaagtttgc gggcgcaaat gcttatacgg gtcggcatcc
     8701 aaatgacgtg atttactacc tgttagcctt ttgcaaggag ttgggctacg actcacgaaa
     8761 tgtaaaggtt tccattacag ggcattcacc catgctagca gagttaaaca tgttggatgg
     8821 ctacttcgac agggttagct caattgccca gtatccaaag gttatgctgt tcagctacca
     8881 gctcatcagc catagaagcg agtacttctc actttttaac ctcggggcat catgcgaata
     8941 gtaggcggct cactaagggg acgaaggttt gatcctccta aaaactttgg ggctcggcct
     9001 actacagatt ttgccaagga aggtcttttc gacgttctga gtaactactt tgattttgag
     9061 gagcttacgg ttttagattt attttccggt acgggcagca taagcctgga gtttgcctcc
     9121 agaggggcac gccatattga tgccgttgaa aaaatttatg ctcatcagaa gtttattaag
     9181 gatagtgcgc tcaggctggg cgttacctcc atatcagtaa tcagacagga tgtattctcg
     9241 tttatcagcc gaagcacctg tcgctatggg ctaatatttg ctgatcctcc ctacgatatg
     9301 gagggcatca gcacgctccc agagttgata ctttcaaagg atttgcttga cgaagatgga
     9361 tggttggtgg tcgagcattc cgaccggtac agcttttcgg ggctaaagca gcttatagac
     9421 caccggcgtt acgggagtgt gaattttagc ttttttagaa aaaatattta ggggaatctt
     9481 gtattattgc tgggaatgtt ctacctttgc aatgttttgt tataagctat tggtccggta
     9541 gttcagtttg gttagaatac atgcctgtca cgcatggggt cgcgagttcg agcctcgtcc
     9601 ggaccgcaaa aaacctcggg atttcccgag gtttttgtat ttaattgctt gtagaaaagt
     9661 caggttaacc gtaccaagcg aaaggttagc ctagttgcag tccttgcact cctccaggtt
     9721 ccggttgtat atagccattg ccttattgct catacccatg ctggagaatc caccatcgtt
     9781 gtatatattc tgcatggtga tttttcgggt gaggtcgcta aatagggtta tgcaaaagtc
     9841 ggcacaatcg tctgcgttgg cgttgcctat tggtgacata cggttggaga aatcgagtag
     9901 ggcatcaaaa cccataacac cgctaccggc agttgtttcg gttggagatt gcgaaacggt
     9961 attaattcta acaccgcgct ccctaccata tatgtaaccg aagctgcggg caatcgattc
    10021 taaaagggct tttgcatcgg ccatgtcgtt gtaaccgtaa agtgtgcgtt gggcagcaat
    10081 ataggagagt gcaacaattg agcctccatg cgtaatagca tcctttttcc agcaggtttg
    10141 aatgattttg tgaaaagata tggcggagat gtccagcgtt ttttgcaggt agtcgtagtc
    10201 cagatcatcg tacgttcttc ctttcctgac attgggcgac atcccaatgg agtgcaggat
    10261 aaagtcgaac tttccaccaa aatgctccat ggtcttgtct gtcaagttct ccaagtcagg
    10321 aatgcttgtg gcatcagcag ggataataat agcgttacat ttcgcagcaa gttcgtctat
    10381 gctgcccatt ctcatggcaa cagcggtgtt agagagcact atttctgcac cctcttcgtg
    10441 agctctctcg gctaccttcc acgcaatgga tttctcgttt aaggcgccaa aaattagccc
    10501 ctttttccct tttagtaagt tgtaggccat atcacaaaat taggtttaac aaatactaaa
    10561 caataaaggt aaatttttgt ttagtttagt ttagtttagg ctaccgtttg attttagcag
    10621 ctctctggca tttaaaattg cagcatcgga catcttttcc ccactaagca ttttggcaat
    10681 ttcaacaacc ctttcttccg gggtgagtag ttttattcct gttgtggagc gcagctcctg
    10741 attttccttg taaaccaaaa agtgtgtttt gcccttagcc gctatttggg gaagatgggt
    10801 gatatttatt acctgcatgt tcttagccat ggtgtggatg atgctgccca tcttatcggc
    10861 aatttctccg gaaacaccgg tgtcaatttc atcaaatatt atggttggaa gtcccgaggc
    10921 tttaaccagt agcgatttta agctgagcat gactcgcgac agctcgcctc ccgaggcaat
    10981 ttttgaaagc tccatgggtg gaatctggct gttggccgag aagtaaaagg ttaccctatc
    11041 ggttccccaa ggttggaatt cgttaacagg ctgaatatca accttaaaaa tggcatgctt
    11101 aattccaagt tggataagca gttctgttat gctttgttca atgggcgctg ctgcttttcg
    11161 ccttaggatt gacagctcgt ctgctttctt tctaagttca tgtcctctac gctccagatc
    11221 cttgcgtctt gtttccaggt taaactccat cccagaaatg tggtttaccc tttcctctat
    11281 cgagttacgg taggcaatca gctcggtcat ggttccctta cggtgttttt gcagtagggt
    11341 gtagagcagg tcgatacgct gcgatacgct agccagcagc tcgtcgtcaa cattaacctg
    11401 tgagctgcgt cgctccgctt catctacaat atccctcagc tcaatgcggc agctttccaa
    11461 acgatgttcc agctcatcgg cagcaggata gtagggcgat atttttttaa ggcttgcaga
    11521 agcttctttt agcagggcta tggccgatgt ttcgtcgttg ctagctgcat ttatggtgaa
    11581 ttccaatgcc gatttaattt cggttgcgtg ggtaagtttt gcttgttcaa tctccagttc
    11641 ctccagctcg ccctctttaa ggttagcagc gcgtagctcc tccagctggt gctgcaggta
    11701 gtcaaaatct cgaccaaaat ttgctgcttc gtcttcaagt ttttttagct cagcctcaag
    11761 ctggcggtag tttaagtacg tttctaggta ggcggattgc aagtttttgg gaatggcgta
    11821 ggtgtcaacc acattaagct gaaagcggga ggttcccagc aggaggtttt gatgctgtga
    11881 atgaatgtct atgaggcggt cacctagttc cttaagaagt gtgaggttaa ccggaatctc
    11941 gtttatgaaa gcccttgatt tgcccgattc gcctatctgt ctgcggacaa tggtagttgg
    12001 gttgtaatcc aaatcgttta ctcggaaaaa gtcatcaagg tcgtagctgc tgatgtcaaa
    12061 ctccccctca actatgcagc tttttgactg gtctttcagt atagacgcat caaccctttg
    12121 gcccagaatg agcatgagcg ctcccagcag gattgatttt ccggctccgg tttcacctgt
    12181 aatgatattc aggccaggtt gaaactctat ggtcagctca tcaattatgg cgtagttttg
    12241 tatgtagagt ttccgaagca tcggcacaag gggtttaggc tagctattct tttgctccag
    12301 tattttctgg tacttgccag cgttggcatt gtcaatttcg gtaagaatgt taactaccct
    12361 ggccttttcg gaggggaacg attccgagaa aatatttacc agctcgtcac ttttggcgtc
    12421 aaagaaaagc tgcagggcaa agaggtaagg gtcgggtttt tcgcggtata ccttttgaag
    12481 ggtggccata cactcagcta tttctgctct accctccact gccttatcgc tcatccggtc
    12541 tagtcctaag cgatggtaac ggtagagcac ctctcgaaga ggccggtatt tggagtttag
    12601 aaggttttca atcatccagt acctgttctt tcggtttccc tcgtaggctt tccatccccg
    12661 ctcaggtgca ttctgggcgt tgttcactac tttttcggct ttaaggagat aatcggtacc
    12721 gccaagcatg gaaaatgaat cgtagtcaat tcctagtata atgtaagcgt agaacgccag
    12781 aatggatgaa agattgctga gatggctgtt ttcggagaac tcaatcttgt caaattctct
    12841 atagcgaaag tgcaggttgt tatcctgaaa gttgagcatg gtggtgttgt aggacgagtt
    12901 gaataccggc cggcgcgatt gtacctggag ggttccctta aattcgtcag atcccagctg
    12961 ttcggtgatg ttaagcatta tgctgcattc aatacgctcg tcgaaaccgt aaacgtggtt
    13021 agtccatgcc gtgttgttca tgaactcgta gatggctgtt tggagcgttt gaaaaatagt
    13081 gcgattagtg ccctgaattt tttgggagtt aatctgcaca ttgcacctta actcctgtcc
    13141 atcgagtacc agcggggcaa tgagcagaag ggttaacatc agcttagcaa aagacttgtg
    13201 catggcgatt cccgttggtt tgtactattt tgcttgttga agcaggtcaa ttatggcgtt
    13261 ggctatatct tgggctacct ctgatttagg ctttagctca aaggattttt gcttaccatt
    13321 gcggtcaatt agggtaatct tgttggtgtt atggccaaaa ccagcgccgg catcgttcag
    13381 ggagttaagt actatgatgt ccaggttttt tcgggttagc ttgtcaatgg cattttcaac
    13441 ctcgttgtgg gtttcaaggg caaaaccgac gatgatttga ttctgccgct tcatcttacc
    13501 gagtgtttcc gcaatatctg ttgtcggctc aagttgtagg tcaagatttt ccttccccct
    13561 tttaatttta tcggttgagg tgttagctgg agcgaaatct gccactgctg ctgcaagaat
    13621 ggcaacctga cagctgtcaa attcattttt tgcctgctcc agcatttcgg aggcggtttt
    13681 aaccctgaca attttaaccg aaggatgttg tggttcaaca gctgccggac cagtgatgaa
    13741 ggtaacctgt gctccttgtt gcgcaatggc ggtggcaatg gcagttccca tttttccgct
    13801 ggaataatta ccaataaagc gaacgggatc gatgggctca taagtaggac ccgaggttat
    13861 tagtaccttg tatccattaa accggttagc cgccaaaaaa aggttgacta cgtgttgtag
    13921 aatattttcg ggttcctcca ttctgccttt accggatagc ccgctggcta gttcccctgt
    13981 agcagcctca acaaaaatat tcccgtaact tttaagaatc tgaatatttt tttgtgtagc
    14041 gggatggcta tacatatcca tatccatggc gggagccagc ataacagggc atctggccga
    14101 gaggtatgtg gtgagcagca ggttatcggc aacaccactt gccatttttg ccatggtgtt
    14161 agccgaggct ggagctatca gaaacaggtc tgcccaaagt cccaaatcaa catgactatt
    14221 ccacccgcca ttctcggggt taaagaagtc aaccagaatg gggttttttg aaagggttgc
    14281 cagggtaagc ggggtgatga actccttggc atgaggggtc ataataacct taacctcagc
    14341 accttcttta atcagcagac gtgtaagaat agctgccttg taggctgcaa tgctgccggt
    14401 aacgccaaga attatatgtt tccccgccag acgcattact ggttggtaac ttcggtgtta
    14461 tcaaccattt tgtagtaaag ctttccatcc ataaattcct gaatggcaat aagggttggt
    14521 ttaggcagct tctcgtagta ctttgaaatt tctatttgct ccctgttttc aaacacctct
    14581 tcaaggttat cggtgtagta ggcaaactcc tcgagcttat ggtttagctc ctgcttaatg
    14641 tcaacgctca gcttgttagc ccttttggca ataattctga ccgtttggta aatattcccg
    14701 gtttccttat ctagctcgtc aaggttgcgc gtgatggtcg aagccggaac gttcattttt
    14761 tttaaatcca tgggtttact attgattgtt agttgatttg caaaactttc attgagctct
    14821 catacatctt tttagcctcg ggcaagtagg gtgattccgg aaactcgcca acaaaggtat
    14881 agtactggtc aatggtattt tggtagcgtt ccctttgctt attgggaatg ctgttttcgg
    14941 caagcaggta cgacgattta accagcaggt aggatagctc ttcacggtac ttactgtttg
    15001 gaaaatcctt caagctgttc tgaatggcga cgattgcagc cttataatcg ccaattttat
    15061 agtatagctt agagctgagg taggactttt ccaccaattt ttccctgagc tcggtgagca
    15121 acccgttaac ctcggtccta cggttggtac taggaaagcg ggtgagaaat tccacgaaag
    15181 cctcaattgc ttcctgggta ctggcctggt cgagttccgg acggggtgaa gatttataga
    15241 agcagtaggc atacatgaac tcggcatcct cggtaaaagc gcttcgttga aatgtctgcc
    15301 taaacttgtc atagctatga ccagcaagca ggtagtcgcc ctggtagtag tagccttgtg
    15361 caatgtagta ttctatagta tccgatctaa aggttccctt gtagatgggc gatagctgct
    15421 ccagcagcgt ggtgtagcgg taatgttctt ttttttcgta gtactccagt gcttttttgt
    15481 acttatactc gtaatcctta ctcttcagga gtttttcata accgctgcaa gaaaccatga
    15541 aaatggtggc aagaattgta aaaagaaata aaattccctt ggtatctctg tgttttgtca
    15601 ttatagggct tgtttaatgc tgcttactct ctttagctga aaattaaaca aactgctatt
    15661 tgagcccttt ttcgtaggct gttttgcagc ttgcagtagc ataaaatagc aggcaaagtt
    15721 ataaaactta gcggtaaatt aaaacttgaa acaagctgta catctaaaaa aggttaatta
    15781 ccctcttttt gcatttattt aggattctcc tgcagggtat cgaggatttt tgggtaagaa
    15841 aggttatatc tttctttgat attttactgt gtgggtcgta gggcagctac ttttccgatc
    15901 aaatgtttga aaaacttgtt tggtattctt ttgtaagatt ggtagtttag gtgatggaac
    15961 cagctgatgc ttacggtttc cctcaggcag aagtttgctg ttggtgagcg ctttctgttt
    16021 gtatgctgct gataatcttt actctacata caagttagct ggctttataa atctttttac
    16081 gggatgtttc aattttttca atattgaaat aaaggacgtt tttttaataa aattgagcta
    16141 gatttgcggc taaattttaa cactttattc attccatctt aacttttaaa atttacagca
    16201 gtggatattt ttgataaaat tgataacatg gggcccattg ggcaatggca aagccagagc
    16261 cacgggtatt tttctttccc gaagttggaa ggagaaattg aaccacacat gatttttagg
    16321 ggcaaaaggg tgctaaactg gagcctaaac aactacctgg ggcttgcaaa cctgccagaa
    16381 gttagaaagg cagatgccga ggctgctgcg cagtatgggt tggcttaccc catgggtgcc
    16441 cggatgatga gtggtcaaac ccgtaagcac gaggagctgg aggaggaact tgccaggttt
    16501 gtaggaaagc cctctgccta cgtactcaac tatggttatc aggggatggt ttctattata
    16561 gatacgctgc ttggcaggaa cgatgtggtt atttacgatt ccgaatccca tgcctgcatt
    16621 attgacgggc ttagaatatt ccccggcaaa cgttttgttt accagcataa cgacatggaa
    16681 agctgccgta agcagctgga acatgctaca gctgttgttg agaaaacagg gggcggcatc
    16741 ttgctagtta cagaaggcgt attcgggatg gccggtgacc ttggtaacct gaaggctatt
    16801 actgacctaa aaaaggagtt taactttcgc attctggttg acgatgctca tggttttggc
    16861 actatgggtg caactggggc aggtacttcc gagcattttg gagtgcagga tcaggttgat
    16921 atctactttg ctacctttgc caagagtatg gccggaatag gagcttttgt tgcctcacaa
    16981 gagcacgtta tccggttcct ggcctacaac atgcgctctc aaatttttgc aaaatcgctt
    17041 cctatggcaa tggtgctggg tattcagaag cggctcgaaa ttttgaaatc ccatcccgag
    17101 cttagggaga atctctggaa tattgcaggc gagctgcaaa aaggacttag ggagataggt
    17161 tttgaccttg gccgtaccca atcgccggtt acgccggttt acatgttcgg cggaatacct
    17221 gaggcc
//
LOCUS       DAOPHX010000043        17200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_3728_length_17200_cov_5.391951, whole genome shotgun sequence.
ACCESSION   DAOPHX010000043 DAOPHX010000000
VERSION     DAOPHX010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 17200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17200
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3728_length_17200_cov_5.391951"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            545..1195
                     /locus_tag="PK502_04810"
     CDS             545..1195
                     /locus_tag="PK502_04810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPH00171.1"
                     /translation="MKILQVVTHFDFGGAENHVRELSNELAAQAHDVYLVGPSGKQIK
                     LLNQKVHFVQASQLSKLLVFQTFFLIYLIKKHQIEVIHAHQRLSILAACWAGFITGTP
                     VVATVHGRVRFDLRSSFSRTRTSKIIFVSHQVLKVSKYYSEINHKSVVIPNGINFQKI
                     IPKRIPYGMAYISRMDSHHKEVVDMLIGLMPDVTASFPQASLWIFGEGKQVEYLKK"
     gene            1363..1644
                     /locus_tag="PK502_04815"
     CDS             1363..1644
                     /locus_tag="PK502_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00172.1"
                     /translation="MGSIVNLRNYSAYRLNNFVDINGIPPTRESLLSEINKFFQNRVQ
                     YKDESILIREKVIQEFNIKTITLQTMNVYSEVTQKQLQKNVTSSSSHTC"
     gene            complement(1645..2106)
                     /locus_tag="PK502_04820"
     CDS             complement(1645..2106)
                     /locus_tag="PK502_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPH00173.1"
                     /translation="MKNIDEIDGKLLNILQDNSRVTIRELSEKLHLSTTPIHERIKKL
                     EKSGIIKQYVTLLDPKILGKKLTVFISVSLSSHVKEVIDEFEREIKVMPEVMECYYVS
                     GTWDFFIKIQCTDMDDYHNFVINRFSRLKNITQFYSSFVMSESKVSYKHQL"
     gene            2373..3857
                     /locus_tag="PK502_04825"
     CDS             2373..3857
                     /locus_tag="PK502_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPH00174.1"
                     /translation="MIKIVDCVSNAKALENAVGRFREKGIILPTIKQQQNPELVPSKI
                     QEQLRGIGLWEINPLNLFRITWKNEPKEKGGLFGGVNFIELPSELTGVKARIVMLIGK
                     WFPTGAHKVGAAYGCLAPQITTGGFDPTTQKAVWPSTGNYCRGGAFDSKLMGADSVAI
                     LPQEMSSERFTWLRDYVGSEVIATPGCESNVKEIYDKCWELRRTKPNYIIFNQFDEFG
                     NSAWHYNVTGKAIEEVFSNLKGGQKNLAAFVSATGSAGTIAAGDYLRTIAPHVKVVAS
                     EALQCPTLYCNGFGGHRIEGIGDKHVPWIHNVKNTDVVTAIDDEDCMRLFRLFNEPEG
                     KSYLASLGIPIETVNTLNLLGISGISNLLSAIKTARYFEMTSEDIIVTIATDSAEMYL
                     SRLEELSNERGAYSQIQAIKDYEKCLLGVSTDWIKELTYTDRKAIHNLKYYTWVEQQG
                     KNVEDLNQLWYDRNIWQNLFNQVSRWDELIEEFNDRTGLLKSLR"
     gene            3882..5120
                     /gene="thrC"
                     /locus_tag="PK502_04830"
     CDS             3882..5120
                     /gene="thrC"
                     /locus_tag="PK502_04830"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPH00175.1"
                     /translation="MNSAKYIYRCCCCGKEYGGSSDIYLCPSCSKLNQPENPPKGVLK
                     VIYDYDSICQNIAQLAGNGYIDLLPIRSLSSLPQLKVGKTPLYKYSRMEGKNLPFTLL
                     LKDDSQNPTFSFKDRASALVSAYALENGKNEIVTASTGNAGSSLAGICASQGQKAIIM
                     VPKNAPAAKLTQIVMYGASIVPVDGSYDQAFDLSVKSSETFGWYNRNTAFNPLTIEGK
                     KTVSFEIFEQLKGQLPDRIFVPVGDGVIISGVYKGFEDLLMLKLIDRMPTIVAVQAKG
                     SSNLVGNLFTDVFCSQTSQTLADSISVDIPRNFYMARQYIKTYNGEAVTVTDGEILEA
                     SAILARNTGLFAEPAASAALAGLISFFRQNKVEGNTSNMVLLTGSGLKDLKAVSPIVQ
                     FPKAIKPKIDALKELVDTWK"
     gene            5256..7823
                     /gene="xdh"
                     /locus_tag="PK502_04835"
     CDS             5256..7823
                     /gene="xdh"
                     /locus_tag="PK502_04835"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HPH00176.1"
                     /translation="MITFTLNSKIVEFTGDPKSNLLVFLRNTMHLTAAKDGCSGQGVC
                     GACSVEVDGKIKMACRTKMGDIEGSVVFTLEGLDETFRKTLGRNFALRGAVQCGFCSP
                     GMIMRAKGLYNQNPKPSRNEIIRAITPNLCRCTGYVKIIDAIDASFKELAGELEPTVV
                     AEAKIGERYPKYQAEATALGERPFVDDLRIDGMLFGALKFSDHPRAEILSIDVAPAMS
                     IDGVVGIYTAKDIPGNHFSGLVFQDWPLMVGKGETTHYIGDVVASVVATTEETARKAV
                     EQIRVTYRVFSPVTDIHQAILPNSPNVHTERSNVLETCTIRFGEVKKAFEEAAYISSD
                     VYETQRIEHAFLETETALALPDGDGVKLYSQGQGVYIDRSQIAKFLDIPEEKVKVVQV
                     QNGGGFGGKEDLTVQGHAALCAYLSGMPIKIHLNREESLRMHPKRHPVWMKMSLACNS
                     KGKFTALKLDAIGDTGAYASVGTKVMERVVGHATGGYTIPAVDISAKTVYTNNIPSGA
                     MRGFGVPQVTFALESCIDDICRQAGFDRWQIRFDNALENGAKTATGQILSGVGLKKCL
                     LAVKDDFISARFAGIAAGLKNTGVGNGMVDDSEVLIKIISGKRVEIHHGWSEMGQGVH
                     TMAIQTLHQEIGIDPSIVEVIVNTEAGVPTGMTTSSRATSLVANALINASAALKADLA
                     EASIEQLAGRTYRGKFVCDWTSKPGAETENPVVHFAYGYAAQVAILDDEGRLRKLVAA
                     HDAGKIMNRMLFEGQIEGALHMGMGYALTEDFPMSEGYPTSYKFNDIGIIRANEMPEL
                     KVIGIEEADPVGPYGAKGIGEIGLVPTAAAIANALMAYDGIKRTKLPMRRSRIKDKE"
     gene            8226..9500
                     /locus_tag="PK502_04840"
     CDS             8226..9500
                     /locus_tag="PK502_04840"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPH00177.1"
                     /translation="MAILLKNATYIEWQTLEFRKTNILVKPGKVDFYDPASIPTGVDE
                     VIDCTGKLVTKSFVVGHHHAYSALSRGMNPPRKTPTNFYEILKYVWWTLDKCLSTEMV
                     EYSGLVTAMACAKAGATFAIDHHASPNAVSNSLSTLASAFEKVGVNHLLCYEISDRDG
                     ELITQQGLEETDRYLDKHQGLVGLHASFTLGDKTLTSAAGLMRKHKSGVHIHVAEGIY
                     DQEHCHQNYGQSVIERLNSFGLLSSSKSILVHCLHLSGNERDIIASSPCWVVQNTDSN
                     LNNNVGYFNSKGLGNNLMLGTDGMHSDMLQSMKSAYFVGQNFDPMSPMQAYSRLRNAH
                     RYLSSNNFSGDDESNLLVIDYNSPTPVTPDNFAAHLVFGINSSHIRDVISNGKLIVKN
                     HRLTLVDEEALLIESRKIAAYLWKRMGDVGAE"
     gene            9556..10194
                     /locus_tag="PK502_04845"
     CDS             9556..10194
                     /locus_tag="PK502_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00178.1"
                     /translation="MNVDILEWIGYTASVIIALSMALNSIVKFRWVNLTGALLFSIYG
                     FLIGALPVGILNGIIVVTDLYYLIRIYSQKDTFEILEIRPDNKYLLRFLDYHSKDIQK
                     YFPNFSYSPDENTISFFVLRNMVVAGVFVARKVADRTLEVQLDYVLPEYRDFKNGRFV
                     YSWLVQNFTEHGFARVWAKAETEKHRNYLLKVGFNITSDGHFYKTLNENQHG"
     gene            10187..11569
                     /gene="hydA"
                     /locus_tag="PK502_04850"
     CDS             10187..11569
                     /gene="hydA"
                     /locus_tag="PK502_04850"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HPH00179.1"
                     /translation="MAEKLEYIIKNGTVVTSTNTQKVDILIKNGKIAALANEFNDDMF
                     QTINANGMYILPGGVDPHVHLHLPTPAGYSSDDFHTGSIAALYGGTTTIIDFVTPHRG
                     QPLTEALAARIEEANECLTDYSFHVSPVDWRRESEDEIKRCIDLGFPSFKIYMAYLDT
                     NGLHDTDIQRVMGVVGRNGGMVTAHCELGEEVDRLRYSFYRQGKTQPIYHQLSRPPQV
                     EVKAVERAIAMAQQANCSLYVVHLSAGSSLEPVHRARAAGQKVYAETCPQYLLLNSSC
                     YTDDFWPAAKYVISPPLRSPSDQEQLWKGIQDEDIQTVGTDHCPFSSSQKAYGINDFR
                     KIPNGAGGIEHRLALLYTYGVLEGNISINRMVDLFSTQPAKIFGLYPQKGEIAVGSDA
                     DLVIWNPAPTSTIQASNHHSQADFNIYEGMKVKGGANFVICHGRVAFANGIEQPELPK
                     GNLLRRKAQG"
     gene            12090..>17200
                     /locus_tag="PK502_04855"
     CDS             12090..>17200
                     /locus_tag="PK502_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00180.1"
                     /translation="MNAPLSNNPQGINIRIVGLLGLLFFLSAEGMAQYAPAGIGTSSE
                     NVLWLRSDQGTSTTTNGEPVSGWNDISGNANHAAQGTAGNQPIYISSGINGLPTIRFD
                     GTNDHLTVADADNLDNTNGVSVFVIAQPVSPDALARGLVSKRVSAGNEEAFYLFTYTG
                     RNLYFNASTYRINGNDPVTDQPQIFSAIYDGSVANPRSKIFYNGEESGNGNGPVSIAN
                     MASDLHIGILNPGYAQAFNGDISEIIVYRDNLNKAQRLIVEAYLANRYNIAITPTSYS
                     STTHTQDFIGIGHSGGDRYSQTRNVGSGLLLSERNETLDETNEFVFAGHDGTPHGFNT
                     NDLPTIPGVNLDQRWDRIYYVERVQNGVIDAGSTDVRITFDFTEAGLSLSTDRVYYLL
                     YRSGTSGTFSSVPGGTGVASNGKVSINLSSSNFASGYYTLARSDQQVKTWYSLNDGNW
                     NDYETWSLKPNEPDNPSHEIPGIMDRAVIQNNKTITVTTDDTQCGILDVIDGIVDFGT
                     TTGSTFSTITGQVNGKIRLAADNFPSGDAGEFADASSGGTVEYYGTGYNLSTSRTFRN
                     MVVNLTNTTNKLYLLSNYTLNGSLTITRGEFYFGDNVSTTSRSLTVYGDFVVEANGKV
                     HTGSANARHQLNLYGDFTNRGNIQLTNRAAADYTAEATDGIVDANFLSATRNQSMLLY
                     GPTRFYRIAISKGLSSTYELYMWASSSAYFELFGYANEGHPDEKQLLVNNNSLGLAYG
                     TVRVGANITVPRLNGGGNYNVSENAILWVDGGTVTKPSGTAVVVYGKVKVSAGTFNAN
                     INSGITTRLNGVFESTGGTTNLGQFRTSVYGTQHIGGFIQSGGNVTVDQAYGSSSDYY
                     LFSLSYEGNIFTLTGGTLHIKGTNAKGSVFINSDPVNQNVSTNATLILESTSTSPFRI
                     TSRAPFPNVTMSRSGSGTRTLVLEGGIVGSNPGNQAELPALPLVTKGTLTINDNVVFD
                     AKGQDVTIGRSYSIGSGANYIAASNTTTFQGASQNYIIAINAAATTKYFHNLTINNNG
                     YTGTLSVSDITVGNNLTITEGSLATGAKTVTVRGNIINSDTIKSTSGGILITNRGIVN
                     LVTVTNGGSYTSVPTVTIAAPGGSGIQATAVAIFNGTPSGSNPLPVARIAITNTGSGY
                     TSAPTVSFSAGAATATASISTTHELGGNGNGVFGSLELNEPHPSEASEKVEVTYLSAN
                     QSITDTLAITDGVLDIKAKSLTLDGYMGSETLTDYSPTKMIRTSGNHSDLGMSRRITS
                     NATYIYPLGIYTKTGDGSRYTPISQAFTSVSDTGYVQLNNVDSELPTLAPSAESALQY
                     YWRVRHSGFSTIPEVYNIFYFYNLPDGYRPGMPDPATSWGTGKIVEITRYYNLGPLSW
                     DNPAKDLSLEYSHQESGQTGSSPPELQEGEFTTGHNTRFAGTVQVFYLRQNGNWRTNA
                     TWSFTRGGAPADDYPKQGDIAVIRRFSVGYNGIVEITNAETCAKVIFDDENGFSSGCP
                     RVQFSNSTAYGSNFAVVEVAETHQGGTPLDGERHGAVIKYDLYDGYAGEFPSGDFGDF
                     NRYPNALVIYTKEGASSEVTLSSSATEYPQVWFDPPANRTFILPDTLVTFNGMVIVPY
                     GHTLRMNTGSSAGATFKRRLNIGHSYGTGYFDFAGNASANQTVTAETNINLYNNAQLR
                     INAPAGIGRTHLLKLHGNMTMETGCSVNLGDGNPA"
BASE COUNT         4942 a         4002 c         3923 g         4333 t
ORIGIN      
        1 cagtgccgct tggacattcc cgcgctacgt tccgcccccg acccatttgc gggtcaggtt
       61 tttatcccgt ttttctaata ccagcaacgt tcaacgattc ccgcctttcc gagcaccact
      121 gagaatatct ttccccagta gggctgaaga caagggtaaa ggctaaagga taaaaaaatt
      181 caagattcaa gaacctgtcc gcctactggc ggaacttgtc cggctcctga cggattcaag
      241 attcaacata cttaacatca atttgttaac aagccaccct tgaaaatttt ctgcatatcc
      301 cgggtatata atgcattcgt gtaaatgctt tttaaggaaa ctgccctttt cattttgaag
      361 ccctgttgag aataattact agggttaaat gaaggggtag cgttggacta actattgaag
      421 gtactacccg tggggagttt tttacatttt gccacggcct tacaaatata cggtttacgt
      481 tttcattact tctgaacttt ttttcatccc caaaaactat atttgccgac acatttgcca
      541 cacattgaaa atacttcaag ttgtaaccca ctttgatttt ggaggtgccg aaaatcacgt
      601 tcgagagcta agcaatgagc tggcagctca ggctcacgac gtttacctgg ttggaccatc
      661 aggaaagcag atcaagcttt taaaccaaaa agttcacttc gttcaagcaa gccagctaag
      721 taagctgctg gtattccaaa cctttttttt gatttacctt ataaaaaaac atcagattga
      781 ggtaattcat gcccatcagc gtctatccat tttagctgcc tgttgggcag gattcataac
      841 aggaacaccc gtagtagcca ctgttcatgg cagagttaga ttcgaccttc gctcatcgtt
      901 ttcacgcacg agaacctcaa aaattatttt tgtaagccat caagtactga aggtatcaaa
      961 atattactct gaaatcaacc acaaatcggt tgtgattcca aatgggataa actttcaaaa
     1021 aattatacca aagcgtattc cttatggaat ggcatacatc agccggatgg atagccatca
     1081 caaagaggtt gttgacatgc ttattggatt aatgcccgat gttacagcca gcttccctca
     1141 ggcaagctta tggatattcg gtgaaggaaa gcaggttgaa tatttaaaaa agtagcatcc
     1201 caactaaacc agagcctaca tagagaggtt gtaagaatat gcgggtacgt tgagaatttc
     1261 aaccagcagg aaattttacc caagctggtg ctaggggttg gaagagtagc tgtagaatcg
     1321 ctggctaacg gcctcaacgt aatttcagtg aactgtaaaa gaatgggcag tatagttaac
     1381 ctacggaact attcggcata ccgtttgaat aattttgttg atataaacgg tattccaccc
     1441 acccgggaat cgcttctgtc agaaatcaat aaattctttc aaaatcgagt tcagtataag
     1501 gatgagtcca tcctcataag agaaaaggta attcaggaat ttaacataaa aacaattacc
     1561 cttcaaacta tgaatgttta ctccgaggta actcaaaagc aactgcaaaa aaacgttaca
     1621 tctagcagta gccacacatg ctagctacag ctggtgctta tacgatactt tcgactcgga
     1681 cattacaaac gagctgtaaa attgcgtgat attctttaac ctcgaaaatc ggtttatgac
     1741 aaagttatgg tagtcgtcca tatcggtaca ttgaattttg ataaaaaaat cccaagttcc
     1801 tgaaacgtag tagcactcca ttacctcggg catcacttta atttccctct caaactcgtc
     1861 tataacctcc tttacatggc ttgaaagcga caccgaaatg aatactgtta gctttttccc
     1921 aagaattttg ggatcgagca gtgttacata ctgcttgata atacctgatt tttcaagctt
     1981 tttaattcgc tcatgaatag gagtggtaga aagatgaagc ttctcggata gctccctgat
     2041 ggttaccctg ctgttgtcct ggagtatgtt tagcaacttg ccgtctattt catcaatatt
     2101 cttcatggga atcaataaat ttatatgatt tatattcagt aaacattact tactaggcac
     2161 acttggctgc aaattaaagt tggcttcgtt taagtttaag gctaacatga tcgataatca
     2221 aaaaagccta tgacttctcc aaaatctcca tacaaaaata tcaattttct caatatttaa
     2281 ttatttatta actttatttc ttatttttat caatattaat agtcatttat tataccaata
     2341 ctaattttgg gaaaatattt tttttacata ttatgataaa aatagttgat tgcgtttcaa
     2401 atgccaaagc gttggaaaat gctgtaggcc gtttcagaga aaagggcatc attctcccaa
     2461 cgatcaagca gcagcaaaac cccgagttag tgccatcaaa gattcaagag cagctaagag
     2521 gtattgggtt atgggaaata aaccccttga atcttttcag gattacttgg aaaaacgaac
     2581 cgaaagagaa gggtgggctc ttcggcgggg ttaactttat tgagctgcct tcggagctta
     2641 ccggcgtgaa ggctcgaata gttatgctta tcggtaagtg gttccccacg ggtgcacata
     2701 aggtaggtgc tgcctatggc tgcttggccc cccaaataac aaccgggggt tttgacccta
     2761 ccacacaaaa agcagtttgg ccatccacag gcaactactg ccgtggtgga gcattcgact
     2821 ccaagctgat gggtgccgat tcagtagcca tccttccaca ggagatgagc agcgaacggt
     2881 ttacatggct tcgcgattat gttggctcgg aggtgattgc gacgcccggt tgcgagtcga
     2941 acgtaaagga aatttacgat aagtgctggg aacttcgccg tactaaaccc aactatataa
     3001 tattcaacca gtttgatgaa tttggtaatt ctgcatggca ctacaacgtt acaggaaaag
     3061 ccattgagga ggttttcagc aaccttaaag gtgggcaaaa aaaccttgct gccttcgttt
     3121 cggcaacagg ttcggctggt accattgccg caggcgacta cctgagaacc attgcgccgc
     3181 atgtaaaagt cgtagcctcc gaagccctgc aatgcccaac actttactgc aacggttttg
     3241 gagggcatcg aatcgaaggc attggtgaca agcatgttcc ctggatacat aatgtgaaga
     3301 ataccgacgt ggtaacggcc attgatgacg aggattgcat gagacttttc aggcttttca
     3361 acgagcctga aggtaagtca taccttgcct ccctgggtat tcccattgaa actgtaaata
     3421 cgcttaacct gcttggcatt tcgggcataa gcaacctgct ctcggcaata aaaacggcac
     3481 gctactttga gatgacatct gaggatataa tcgttaccat agcaaccgat tctgccgaaa
     3541 tgtacctttc ccgacttgaa gagctgtcca acgagcgtgg tgcttactcc cagattcagg
     3601 caattaagga ttacgagaaa tgcctgcttg gcgtatcaac cgactggatt aaagagctaa
     3661 cctataccga ccgcaaggct attcacaacc tgaagtacta cacatgggtt gagcaacagg
     3721 gcaagaatgt tgaagacctc aaccagctct ggtacgacag gaatatctgg caaaaccttt
     3781 tcaaccaggt tagccggtgg gatgagctga ttgaggaatt taacgacaga accgggctac
     3841 ttaagtccct caggtagccg caaaggctta tagataatcc gatgaacagt gcaaagtata
     3901 tttaccgatg ctgttgctgt ggaaaggagt atggcggctc gtcagatatt tacctttgcc
     3961 cgtcgtgcag taagctaaat caacccgaaa acccgcctaa aggcgtactt aaggtaatct
     4021 atgactacga ctccatttgc caaaacatag cgcagctagc aggaaacgga tacatcgatc
     4081 ttttaccaat acgctcacta tccagcttgc ctcagctaaa agttgggaaa acccctcttt
     4141 acaagtacag ccgtatggag gggaaaaatc ttccttttac cctgcttctg aaggatgact
     4201 cacaaaaccc tacattttcg ttcaaggaca gagcttcggc attggtttcg gcctatgccc
     4261 tcgaaaatgg gaaaaatgag atagtaaccg catcaaccgg caatgcaggc tcatcgctag
     4321 ccggtatttg cgcctctcag ggtcaaaaag ccattattat ggtccccaaa aatgctcctg
     4381 cagccaagtt gacgcaaata gtaatgtatg gcgcatcaat tgttcctgtt gatggatcct
     4441 acgatcaagc tttcgacttg agtgtgaaat catccgaaac atttggctgg tacaacagga
     4501 ataccgcttt taacccgcta accattgagg ggaaaaagac cgtttcgttc gaaatctttg
     4561 agcagctgaa gggccagctg cccgatagaa tttttgttcc cgttggcgat ggcgttatca
     4621 tctcaggtgt ttacaaggga tttgaggatc ttctgatgtt gaagttgatt gacagaatgc
     4681 ctaccatcgt tgccgttcag gctaaaggta gtagcaatct tgttggtaat cttttcaccg
     4741 atgttttttg ttcgcaaacc agccaaaccc tggctgattc catttcggta gatattcccc
     4801 gtaacttcta catggcccgt caatacatta aaacatacaa tggcgaagct gtaacggtta
     4861 ctgatggtga aattcttgaa gcctcagcca ttctggcaag aaataccgga ctctttgccg
     4921 aacctgctgc ttcggcagca cttgcaggcc taatatcgtt cttcaggcaa aataaagttg
     4981 aagggaatac ctcaaacatg gttctgttaa ccggcagcgg tctgaaagac ctaaaagccg
     5041 tttcgcccat tgttcaattt ccaaaagcca ttaaaccaaa aattgatgct cttaaggaac
     5101 ttgttgatac ctggaaatga atagtttaag ccaaaatagt gcattttaac caaaatcaac
     5161 cagctaatag gcaaacgcaa tcccttcaaa ttagtaaatt gcagcttgtt cctggataag
     5221 acagctattc gagttcaatc atcttctaaa aatctatgat aacttttacg ctaaactcca
     5281 aaattgttga gtttacagga gacccaaaaa gcaacctgct ggtttttctt cgaaatacaa
     5341 tgcaccttac tgcagccaaa gatggatgtt ccggacaggg tgtatgtgga gcatgttcag
     5401 ttgaagttga cggtaagatt aaaatggcct gccgaacaaa aatgggagac attgagggat
     5461 cggttgtctt caccctcgag gggctcgatg aaacatttag gaaaacgttg ggccgaaatt
     5521 ttgccctcag gggtgccgtt cagtgtggtt tctgctcacc tggtatgatt atgagggcaa
     5581 aggggcttta caaccaaaac ccaaaaccct ccaggaacga gattataaga gcaataaccc
     5641 ctaacctttg caggtgtacc ggatacgtta aaattattga tgctatagac gcctcattta
     5701 aagaacttgc aggcgagtta gaacctactg tcgttgccga agcaaaaatt ggagaacgct
     5761 atccaaagta ccaggccgaa gcaacagctc tgggcgagag gccctttgtt gacgacttac
     5821 ggatagacgg aatgcttttt ggcgcattga aattctccga ccatccacgt gccgagatac
     5881 tcagcattga tgttgcccct gctatgagca ttgatggggt ggtaggaata tacactgcaa
     5941 aggatatacc gggtaaccac ttcagcgggt tggtatttca ggattggccg ctgatggttg
     6001 gtaaaggtga aacaacacat tacattggcg atgtagtagc cagtgttgta gccacaactg
     6061 aggagacagc acgcaaagct gtagagcaaa tcagggtaac ctacagggtt tttagcccgg
     6121 taaccgatat tcatcaagcc atacttccta acagtccgaa tgttcatact gaaaggtcaa
     6181 acgtgctgga aacctgcaca atacgttttg gtgaggtgaa aaaagcattc gaggaagcag
     6241 cctacatttc atcagatgtc tatgaaacac agcgcataga gcatgcattt ctggaaactg
     6301 aaacagcgtt ggcattaccc gatggtgatg gtgtaaagtt gtatagccag gggcaaggag
     6361 tttacataga cagaagtcaa attgccaaat ttcttgatat tcccgaggaa aaggtaaaag
     6421 ttgttcaggt tcagaatgga ggagggtttg gaggtaagga ggacctaacc gtgcaggggc
     6481 atgccgccct gtgtgcctat ctctccggaa tgccgattaa gattcatctg aatagggagg
     6541 aatcgctgag aatgcatcct aagaggcatc ctgtgtggat gaaaatgagc ttggcttgca
     6601 actcaaaggg aaaattcacg gcgcttaaac ttgatgctat tggcgatacc ggagcttacg
     6661 cctcggtggg aacaaaggta atggaacgcg tggtggggca cgccacaggg ggatacacca
     6721 ttccggctgt tgacatatcg gccaaaactg tttacaccaa caacatccca tcaggagcta
     6781 tgcgaggttt tggagtaccc caggtaacct ttgccctgga gagctgtatt gatgatattt
     6841 gccgacaggc aggtttcgac agatggcaaa ttcgctttga caatgctctg gagaatggtg
     6901 ctaaaaccgc tactggacag attttaagtg gtgtaggatt gaaaaagtgc cttttagcag
     6961 ttaaggatga cttcatctcg gcaaggtttg caggcattgc cgcaggactt aaaaataccg
     7021 gcgtaggcaa cggtatggtt gacgatagcg aggtactcat aaagatcatt tcgggtaagc
     7081 gtgttgaaat tcaccatggc tggtcggaaa tgggacaggg agttcacacc atggcaattc
     7141 agacacttca tcaggaaatc ggtatagacc cttcgattgt tgaagtaata gtaaacaccg
     7201 aagccggtgt tccaaccggt atgacaacct cctcaagggc tacctcactt gttgccaatg
     7261 ccttaatcaa cgcttccgca gctcttaaag ccgatttagc tgaagcttca atcgaacagc
     7321 tggcagggag aacctacagg ggcaaattcg tctgcgactg gacgagtaaa ccgggtgccg
     7381 aaaccgaaaa ccccgttgtt cattttgctt atgggtatgc agcccaagtg gcaatccttg
     7441 atgatgaggg acgtttaaga aaactagttg ccgcccacga tgccggtaaa attatgaacc
     7501 gcatgctctt tgagggtcaa attgaaggtg ccctgcacat gggaatgggc tacgccctca
     7561 ccgaagattt ccctatgagc gaaggctatc ctaccagcta caaattcaac gatattggaa
     7621 tcataagagc taacgaaatg cccgagttaa aggtcatagg aattgaggaa gccgatcctg
     7681 ttggccctta tggtgcaaaa ggaattggtg aaattggctt agtacccacc gctgcagcta
     7741 ttgcaaatgc attaatggcc tacgatggga taaagaggac aaagctaccc atgagaaggt
     7801 ccaggataaa ggataaagaa taaagaataa aaaaatggct caatttaaag aaaatgtttt
     7861 tccccgataa ccataacgaa atcatgaata gtaatctttg cgttctgtaa tatctttttg
     7921 tgctttgtgg tttctagtat tatttaacca caaagttcac aaagaaaagt ttaacaaact
     7981 tatcatatag agtgataatc ggtataacaa cattattttt ttaatagagc caaagagata
     8041 aagggtaaaa gaaaccttcg gctaaaaggg tttaccctac aacaggtgtt acgtcgagta
     8101 gttcggctgg tgttatagtg agccagtcga actactcact ataacacctg ccgagctgct
     8161 caggacaggc ttttatagct tctcatggat tataagtttt gaaacacata aaaattacat
     8221 aaacgatggc catactgcta aagaacgcaa catacattga gtggcaaacg cttgaattca
     8281 ggaaaactaa cattttggta aagccaggga aagttgattt ttatgatccg gcaagcattc
     8341 ctactggggt tgatgaggta atagactgta cgggaaagtt ggttaccaag tcgtttgttg
     8401 tggggcatca ccatgcgtac tccgccctat cgcgcgggat gaatccaccc cgaaaaacgc
     8461 ccaccaactt ttacgaaatt ttaaagtatg tttggtggac cctcgataaa tgcctgtcaa
     8521 ctgaaatggt tgagtatagc ggacttgtta cagccatggc gtgcgccaaa gctggcgcaa
     8581 catttgccat tgaccaccat gcctcaccta atgctgtgag caattcgctt agtacgcttg
     8641 ccagcgcatt tgaaaaggtt ggcgtcaacc atctgttatg ctacgaaata agcgatcgtg
     8701 atggagagct aatcacccag cagggattgg aagagaccga ccgctacctg gacaaacacc
     8761 agggattggt gggactacat gcctcgttta cactgggtga taaaacactt acttctgccg
     8821 cagggctgat gcgcaagcat aagagtggcg ttcatattca tgtggcagaa ggtatatacg
     8881 atcaggagca ttgccaccag aattatggcc aatcggtaat cgaaaggcta aatagcttcg
     8941 ggttgctaag ctcctccaag tctattctag ttcactgcct ccacctctca ggtaacgaac
     9001 gagatataat tgccagctcc ccatgctggg tggtgcagaa caccgatagc aacctgaaca
     9061 acaatgtagg ctacttcaac tcaaagggcc tgggcaataa cctcatgctg ggaaccgacg
     9121 gaatgcacag cgacatgctg cagagtatga aatcggccta cttcgtaggg caaaacttcg
     9181 accccatgag ccccatgcag gcctacagcc ggttgagaaa cgctcatagg tatctttcct
     9241 ccaacaactt ttccggcgat gacgagtcga acctgttggt aattgactac aactccccca
     9301 ccccggttac ccctgacaat tttgctgccc acctggtttt cggtattaac tcctcgcaca
     9361 tacgggatgt aatttcaaac ggtaagctta ttgttaaaaa ccaccgatta acactggttg
     9421 acgaggaggc cttactaatc gaatccagaa agattgcagc ctacctatgg aaacgtatgg
     9481 gcgatgtagg agctgaatga atttgctgac actacgtatt aattgcctac ctttgccaaa
     9541 caaattctac ctcctatgaa tgttgatatt ttggaatgga ttggctatac ggcttcggtg
     9601 attattgccc tttcaatggc gctcaactcc attgtcaagt tccgctgggt aaacctcacc
     9661 ggtgcattac ttttctcaat atatggcttt ctaataggtg cactaccagt gggtattctc
     9721 aatggaatta ttgttgttac cgacctttac taccttataa gaatttactc ccaaaaggat
     9781 acattcgaga ttctcgaaat aagacccgac aacaagtacc tactgcgatt tctagactac
     9841 cactcaaagg atattcagaa atactttccc aacttttcct actcccccga tgagaacacc
     9901 attagcttct ttgtgcttcg taacatggta gtggctgggg tgtttgttgc ccggaaggtc
     9961 gctgatagaa ccctggaggt tcagcttgac tacgttttgc ccgaatatag ggattttaaa
    10021 aatggaaggt ttgtttactc gtggctagtg caaaacttca ccgagcatgg ttttgccagg
    10081 gtttgggcta aggctgaaac cgaaaagcac agaaactacc tacttaaggt gggttttaac
    10141 attaccagcg atggccattt ttacaaaact ttaaacgaga accaacatgg ctgaaaaatt
    10201 ggaatacatt attaaaaatg gaacggttgt tacttctact aatacccaaa aagttgatat
    10261 tcttattaaa aacgggaaaa ttgcagcatt ggccaacgag tttaatgacg atatgttcca
    10321 gaccattaat gccaatggta tgtacatact tccaggaggt gttgacccgc acgtccatct
    10381 gcatcttcca actcctgctg gctactcatc ggacgatttt cacacgggta gcatagctgc
    10441 cctatacgga gggacaacca ctattattga ttttgttacc ccgcataggg ggcaacccct
    10501 tactgaggcg ttagctgcaa gaattgaaga agctaatgag tgtctcacgg attactcctt
    10561 tcatgttagt ccggttgact ggcgcaggga aagtgaggat gaaattaagc ggtgcataga
    10621 tttaggattt ccatccttta aaatctacat ggcttacctt gataccaacg gccttcacga
    10681 tacggatatt caacgggtaa tgggcgtagt cggaaggaat ggaggaatgg ttaccgctca
    10741 ctgcgagctt ggcgaagagg ttgacaggct gcgctactcc ttttaccggc agggcaaaac
    10801 tcagcctatc taccaccaac tttcacgtcc accgcaggtg gaggttaagg ctgttgaacg
    10861 tgccattgcc atggcacaac aggcaaattg ttccctctac gttgttcacc tgtcggcagg
    10921 cagctctctt gagcctgttc atcgtgcaag agctgccgga cagaaggttt atgccgaaac
    10981 ctgcccgcag taccttctgc tcaacagctc ctgctacacc gacgattttt ggccagctgc
    11041 aaagtatgtt attagcccgc cgctccgctc accgagcgat caggaacagc tttggaaagg
    11101 cattcaggat gaagacatcc agaccgtggg aaccgaccat tgccccttca gttctagtca
    11161 aaaggcctat ggaataaacg atttccgaaa aatacccaat ggcgcagggg gaattgaaca
    11221 tcgtttagct ttgctctata cgtatggcgt tttggagggg aacattagca tcaaccggat
    11281 ggttgacctc ttttcaaccc aaccggcaaa aatattcgga ctttatcccc aaaagggcga
    11341 aatagctgtt ggctccgatg ccgacctggt tatttggaat ccggccccca cctcaaccat
    11401 tcaggcaagc aaccatcatt cgcaggcaga cttcaacata tatgaaggca tgaaggtgaa
    11461 aggcggagct aacttcgtta tctgtcatgg acgagttgcc tttgcaaacg ggatagagca
    11521 accggaactc cccaaaggaa acctattgag gagaaaagca caaggttaaa ggttaataca
    11581 aggataaagg ttataggttc aaacttcgac aagctcaggg aaaaaaatcc aagattcaga
    11641 acaatttcag gatgtgtttt aagcccagaa ggggcgaaac atgattaacc ccaggcgcaa
    11701 gcccggggta tgacgcggta ggtgaagaat tggcgatgca agtagcaaat cttccaaagc
    11761 gcaaccctga ccagttgcat cgcaacgcag ggaattttcc aaagcgctgg gacaaaaaag
    11821 ggataaagac agggatgaag ttgctttgaa gtgaagataa aatccttcac ctgcggaaat
    11881 taatcaaaag tcaattcttt aatcttcttc cctttaactt ttcacattta gtttgtatat
    11941 ttttacataa cagctcacaa taatgcccgt ttttgaacac taattggtct gctatttttc
    12001 cttttctgga ataactaact gatatactta tttttattaa atttggcgta aatattgcaa
    12061 ttttatgata actacaaaat agccacaaga tgaatgcgcc tctgtccaat aatccacaag
    12121 gcatcaacat aaggatagtg ggccttttag gcttactgtt tttcctctca gctgaaggaa
    12181 tggcccagta tgctccggcg ggcataggca caagcagcga aaatgtgctg tggctgcgct
    12241 ccgaccaggg aacctcaacc accacaaacg gagaaccagt gagtggctgg aatgatatta
    12301 gcgggaatgc aaaccatgcc gctcagggaa cagcgggcaa ccagcccatt tacatttctt
    12361 cgggaattaa cggcttgcct acaattcgat ttgatggaac aaatgaccat ttgaccgttg
    12421 ctgatgccga caaccttgac aataccaatg gggtaagcgt ttttgttatt gcccaacccg
    12481 ttagtccgga tgctttggca agaggactgg tttccaaaag agtaagcgct ggaaatgagg
    12541 aggccttcta tctattcacg tacaccggta ggaacctata ttttaacgcc tcaacttacc
    12601 gaatcaacgg caacgatcct gtaacagacc aaccccagat attttcggcc atttacgatg
    12661 gatcggtggc taacccacgt tcaaaaatct tttacaatgg tgaggaaagc ggcaacggaa
    12721 acgggccggt tagcattgca aatatggcct ccgacctgca cattggaatc cttaaccccg
    12781 gatatgctca ggcatttaac ggcgatattt cggagattat tgtgtacaga gacaacctta
    12841 ataaggcgca aaggttaatt gtagaggctt acctggctaa tcgttacaac attgccataa
    12901 cgccaacctc ctactcttca accacgcaca cgcaagattt tattgggatt ggtcattcgg
    12961 gtggcgatag gtacagccaa accagaaatg ttggctccgg cttgctccta tccgaacgca
    13021 acgaaacgct cgatgaaacc aatgagtttg tttttgccgg acacgatggc acaccccatg
    13081 gcttcaatac caacgaccta ccaaccattc ccggtgttaa ccttgatcag cgttgggata
    13141 ggatttacta cgttgagcgg gtccagaatg gagttattga tgctggaagc accgatgtac
    13201 ggattacctt cgactttacc gaggctggac tatccctttc gaccgacagg gtttactacc
    13261 tgctctaccg atctggaaca tccggaacct tctcctctgt ccctggtgga accggggttg
    13321 cctccaacgg aaaggtttct atcaatctga gcagctctaa ttttgccagc ggatactata
    13381 ccctggcaag aagcgaccag caggtaaaaa catggtactc gctaaacgac gggaactgga
    13441 acgattacga aacatggagc ctaaaaccca acgagcctga taacccctcg catgaaattc
    13501 ctggaatcat ggatagagcc gttatacaga acaataaaac cattactgtt actacagatg
    13561 atacccagtg cggaattctt gatgtgattg acgggattgt agattttggt accacaaccg
    13621 gcagtacgtt ctcaaccatt accggccagg taaacggaaa gataaggctg gcagccgata
    13681 attttccatc gggcgatgcc ggtgaatttg ccgatgcctc ctcgggcgga accgtggagt
    13741 actacgggac aggatacaac ctttcaacct cccgaacctt ccggaatatg gttgtaaacc
    13801 tgaccaacac caccaataag ctctacttgc tgtcgaacta caccctaaac ggctcgttga
    13861 ccattaccag gggtgagttt tattttggtg ataacgtctc cacaacctcc cgctcgctaa
    13921 ccgtttacgg ggattttgtg gttgaggcca atgggaaagt tcatacgggt tccgcaaatg
    13981 cgcggcacca gcttaacctt tatggcgatt ttaccaaccg tggtaacatt caacttacca
    14041 accgtgctgc ggcagactac acagccgagg caaccgacgg gattgttgat gccaacttcc
    14101 tgagcgccac gcgtaaccag agcatgttgc tatacggccc aacccgtttc taccgtatag
    14161 caatcagcaa gggactctcc agcacctacg aactctacat gtgggcatcc agctcagcct
    14221 acttcgagct ttttggctat gccaacgagg ggcaccccga cgaaaagcag ctgttggtta
    14281 acaacaactc gttaggcctg gcttacggaa ccgtaagggt tggggcaaat ataacggtgc
    14341 cccgtttgaa tggaggaggc aactacaacg tttcggaaaa tgccatcctt tgggttgatg
    14401 gcggcacagt tacgaaacca agcggaaccg ctgtggtagt ttatggcaag gtaaaggttt
    14461 cggcaggaac gttcaacgct aacataaaca gcggaatcac caccaggtta aacggcgtgt
    14521 ttgagtccac cggtggcacc accaacctgg gccagttcag aacatccgtt tatggaaccc
    14581 agcacattgg cggattcatt cagtccggag gcaatgtaac cgttgaccag gcctacggtt
    14641 catcttctga ctactatctc ttctcgctat cctatgaggg aaacatattc accctgaccg
    14701 gtggaacgct gcacatcaag ggaacaaacg ccaagggttc ggtttttatc aattccgacc
    14761 ctgtaaacca gaatgttagc accaacgcca ccctgatttt agaatccacg agcacttccc
    14821 ctttccgcat tacctcacga gctccctttc caaatgtaac catgagccgt tccggctcag
    14881 gaacaagaac cttggtgctt gaggggggaa ttgtaggaag caatcccggt aaccaagctg
    14941 agctgccggc cctacccttg gtaacaaagg gaactctgac aataaacgac aacgtagtat
    15001 tcgatgccaa ggggcaagac gtaactattg gtcggagcta cagcattggt tcgggagcca
    15061 actacattgc agcaagtaac acgacaacct tccagggggc atcccagaac tacattattg
    15121 ctataaatgc ggctgcaact acaaagtact tccataatct cacaataaac aacaatggtt
    15181 acaccggaac cctttcggta agcgacatca cagttggtaa caatttgacc attaccgaag
    15241 ggtcgcttgc aaccggcgct aaaacagtaa ctgtgcgcgg caatatcatt aactccgaca
    15301 cgattaaaag cacatcagga ggtattctta tcacaaaccg tggcattgtg aatctggtta
    15361 cggttaccaa tgggggcagc tacacctccg ttccaacggt aaccattgca gctcccggcg
    15421 gatcgggaat ccaggccact gctgtggcca tattcaacgg aactccctct ggatctaacc
    15481 cgctcccagt ggccagaata gccataacca acaccggtag cgggtacacc tcagccccaa
    15541 cggtgagctt cagtgctggg gcagccactg ccacggcatc catctcaaca acccatgagc
    15601 ttggtggcaa cggcaacggc gtatttggca gcctggagct gaacgaaccc cacccaagcg
    15661 aagcatccga aaaggtggag gtaacatacc tgtctgcaaa tcaatctatt accgatacgc
    15721 tagcaataac agatggcgtg ctcgacataa aggcaaagag cctgacgctt gacgggtata
    15781 tgggtagcga aacccttaca gattacagcc ctaccaaaat gatccgtacc tcgggcaacc
    15841 actccgattt aggaatgtcg aggcgtataa cctcaaacgc aacctacata tatcctcttg
    15901 gaatatacac aaaaactggc gatggaagcc gttatacccc cattagccag gccttcacct
    15961 cggtttctga taccggctac gtgcagctga acaatgtgga tagcgagctg cccaccctgg
    16021 cgccatcagc agaatcggcc ctgcagtact actggcgggt tcggcattcg ggattttcca
    16081 ccatacctga agtttacaac attttttact tctataatct ccctgatggc tatagacccg
    16141 gaatgccaga ccccgctacc tcatggggaa caggaaagat agtggaaatc acccgatact
    16201 acaacctggg tcccctgagt tgggataacc cggccaaaga tctttccctg gagtactcgc
    16261 accaggagag cgggcaaacc ggaagctcac cgccggaact ccaggaaggg gaatttacca
    16321 cggggcataa tacacgtttt gccggaacgg ttcaggtttt ctacctacgt caaaatggaa
    16381 actggaggac taatgcaaca tggtcgttta cccgcggggg tgcccctgct gatgattacc
    16441 ccaaacaagg ggatatagcg gtaattcgga ggttctctgt cggatacaac ggtattgttg
    16501 aaattacaaa tgccgagaca tgcgcaaagg tgatatttga cgatgagaat ggcttttcaa
    16561 gcggatgccc cagggtacag ttctccaact caacggccta cggaagcaat tttgccgttg
    16621 tagaggttgc agaaacccac cagggaggca cacctctgga tggggaacgt catggagcag
    16681 ttatcaagta tgatctttac gatgggtacg ccggagaatt cccctcggga gactttggcg
    16741 attttaaccg gtacccaaat gctcttgtaa tatacaccaa agaaggagca tcatctgaag
    16801 taaccctttc ctcttcggca acggagtacc cccaggtttg gtttgacccg ccagccaaca
    16861 gaacatttat cctgcccgat actcttgtta cctttaacgg tatggtaatc gtgccctacg
    16921 ggcataccct acgaatgaat actggctcaa gtgcgggggc aaccttcaaa agaaggttaa
    16981 atattggaca ttcttatggc acgggttact ttgattttgc aggtaatgca agcgccaacc
    17041 aaaccgtaac ggccgaaacc aacatcaacc tttacaacaa cgcccagctt cgtattaatg
    17101 ccccagccgg cattggcaga acacaccttc tgaagctgca tggcaacatg actatggaaa
    17161 caggttgctc tgttaacctg ggagatggta accctgcaaa
//
LOCUS       DAOPHX010000044        16917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_3821_length_16917_cov_4.718539, whole genome shotgun sequence.
ACCESSION   DAOPHX010000044 DAOPHX010000000
VERSION     DAOPHX010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 16917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16917
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3821_length_16917_cov_4.718539"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(369..3239)
                     /gene="gcvP"
                     /locus_tag="PK502_04860"
     CDS             complement(369..3239)
                     /gene="gcvP"
                     /locus_tag="PK502_04860"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HPH00181.1"
                     /translation="MTTDNFVARHNGPRDHEIDEMLKEIGVSTLDELINQTVPQNIRL
                     RKPLNLPNPVSEYEFLGRMKKIAAKNKLYKSYIGMGYYGTVTPSVILRNVFENPGWYT
                     SYTPYQAEISQGRLEALLNFQTMVIDLTRMEIANASLLDEATAAAEAMIMMHNLRSRE
                     AVKAGKNILFVDRDIFPQTLAVITTRSKPLGIDVVTGCYSEYSFTGREFGVVIQYPSA
                     KGNIRDYSSFTAKAHEAGTLVTVMADLLSLALITPPGEWGADIVAGSTQRFGIPMGFG
                     GPHAAYFACKDEFKRSMPGRIIGVSIDRHGNKALRMALQTREQHIKRERATSNICTAQ
                     ALLATMAGMYAVYHGPSGIRRIASYIHSVAMLVAKQLQLLGYTQNIKNIFDTLEVTLP
                     AGVSLEDIRRNALENELNLNYKPNGSIGISFDEVTTINDINALLNVFAKAIGKEHNPI
                     SSVEEANGIPANLQRRSPYLTEKVFNSYQSETEMMRYIKKLERRDIALNHSMISLGSC
                     TMKLNPAATMLPLSWPEMANIHPFVPADQAAGYHEIISELEHLLAEITGFDSVSLQPN
                     SGAAGEYTGLMVIREYHLSHGDSKRNVVLIPSSAHGTNPASAAMAGMQVVVVACDEMG
                     NINVDDLKAKAEQYRETLSAFMVTYPSTHGVFESKIREMVDIIHKNGGLVYMDGANMN
                     AQVGLTSPGEIGADVCHLNLHKTFAIPHGGGGPGVGPIAVAKHLTKFLPSHPVVKTGG
                     ENGISAVAASPWGSASILPISYGYILMLGAEGLTQVTKMAILNANYMAAKLKKHFKIL
                     YTGETGRVAHEMILDCHHFKTTYGVDTSDVARRLMDYGFHAPTVSFPVHESLMVEPTE
                     SESKQELDRFIEAMISILAELEDIKAGKNATPTDNLVLNAPHTAPEVSADEWHHPYSR
                     QKAAYPLTWIASNKFWPYVSKIDSGYGDRNLVCTCAPIEDYL"
     gene            complement(3355..3993)
                     /locus_tag="PK502_04865"
     CDS             complement(3355..3993)
                     /locus_tag="PK502_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPH00182.1"
                     /translation="MKVKVFVFNPFQENTYVISDENHNAAIVDAGCYNATEVALIKRF
                     VDDNELKVKYFVNTHCHVDHLLGLNLLKSIYNVEAYAHSDDLPLLKTFPQQAMSFGFS
                     VDKTPEIDKIVADGNTLLLGDGSIGIIHTPGHTPGSICLYLQNEKMLLTGDTLFYRSI
                     GRTDLPGGSYDAIIRSITTKLMVLPDDVAVYPGHGESTTIGDERRSNPFIKT"
     gene            complement(4239..4892)
                     /locus_tag="PK502_04870"
     CDS             complement(4239..4892)
                     /locus_tag="PK502_04870"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPH00183.1"
                     /translation="MQQDTYKHKGLRKRLIEEIESKGITDKQVLGAMLRVPRHFFMES
                     SFLKFAYSDQAFPIGYGQTISQPFTVAFQTQLLQVEHMNKVLEVGTGSGYQAAILMEL
                     GVRLFTIERQRELYLNAKSLLEEMGYHPSFHFGDGYKGLPGYSPFDRIIVTAGAPTIP
                     DELVDQLAVGGRMVIPVGDENGQVMTLVEKISSTEVRKTTHGNFIFVPLLKGVEKSK"
     gene            5140..6003
                     /locus_tag="PK502_04875"
     CDS             5140..6003
                     /locus_tag="PK502_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00184.1"
                     /translation="MLKLGFFTDNTQLAELVGDLGRQANIEVEGIWAEPLLNGDDKRN
                     FSNCFSDPEKLYGSIDVGVISISSSELFMDIVTAAIKNGVHPLVVGLPDLQLSTYNQL
                     SLLSAEMGVELGFSHIYHRFSGFNYPLVSPIIAHLTRGITMNQLNQFEMAATLRFDIA
                     TILSLTTSEVRRIRTYVLPQSGECPSCMMVFIDFFNNSAITYNLTLGAKRNFLELRLN
                     SIGEAIELYEDEIKLATSSLGGMISTSIFLDDLNNFTGCIGIQREPLLGISFVMKILS
                     AVEAISQKMAR"
     gene            6059..7474
                     /locus_tag="PK502_04880"
     CDS             6059..7474
                     /locus_tag="PK502_04880"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPH00185.1"
                     /translation="MTKNYLKIWYILFDVVGSAAAWTLFCIYPAKDVGLEFLRAGATF
                     EFDGKFYASLVFFVLFWLALFIVAGFYNNLYRKSRLKEFTKSVGYTFVGTVVIFFILL
                     LDDSLATYNDNFSIIGSMFILELTFTYFPRLVLTTGTIRKIHRRIIGFPTIIVGNGFK
                     ALQVYRELEAEKKSQGFRFVGYIAQVNNHEDMLQSQQKRLGEMDSIAEVVKKQGVEEV
                     ILAVEGEDVSTLTRCINELLPLKVNLRAIPSMYDFLLGRVKMTGILNAPLISVSFDLM
                     PLWQANIKRIADYTVSALAIVLLLPVSLILGIAIRCTSQGPIIFKQERIGQYGRPFWI
                     YKFRSMYADAEKSGPTLSGKDDPRVTPIGRFMRKTKLDEIPNFINVLKGEMSLVGPRP
                     ERQCYIEQIVRIAPHYVHLQKVKPGITSWGQVKFGYAKNVDEMVERLKYDILYLENMS
                     LFVDLKIIIYTILTIFKGKGL"
     gene            complement(7471..8037)
                     /gene="thpR"
                     /locus_tag="PK502_04885"
     CDS             complement(7471..8037)
                     /gene="thpR"
                     /locus_tag="PK502_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPH00186.1"
                     /translation="MKKERTFIAVGIKPEAKLLNCWHKLKQQHNLDFINWVDEGMLHL
                     TLRFLGEIPVDLVGDVSKVLSISLTGFNSFSLKVKELGSFGRPTPRVIWVGIEQNETL
                     NNLKQTIDKALLPLGFDREKGLFMPHITLGRVNFIRNPRAFYDMTEKLQNYTFQESEI
                     EEVTFYQSLLTPKGAIHKPIKNFLLLKG"
     gene            8122..8778
                     /gene="elbB"
                     /locus_tag="PK502_04890"
     CDS             8122..8778
                     /gene="elbB"
                     /locus_tag="PK502_04890"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HPH00187.1"
                     /translation="MANSKKFAVVLSGCGVLDGSEIHEAVMTLYAITLSGSSYEIFAP
                     DVNQHHVVNHLNGSEVDQTRNVLVESARIARGKIKPLGDFSSDVFDAIIFPGGFGVAK
                     NLSSWAFDGAGCSVNADVANAVRSMHRLHKPIGALCIAPVVIARILGGVTVTIGKDAA
                     TVKEIETLGARHQMALQTQVVVDEKNMIFSTPCYMLDATIVDIAEGAKAIVSEMLKWI
                     "
     gene            complement(8791..9417)
                     /locus_tag="PK502_04895"
     CDS             complement(8791..9417)
                     /locus_tag="PK502_04895"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HPH00188.1"
                     /translation="MRSSRINMTKSKLLPLAILLGITLSSGHSHAQVRDSVVVNLYTI
                     VFYEGDTIPYIKLREVVKYGKRRFKSRSEMNQYYKMIRNLKLTYPYAQLAKYKLLEMN
                     EDLKMIKTERERREYINQAEKELRMQYEKDLRKFTVSQGKMLMKLIDRETGATSYDLV
                     KELKGGFSATFWQGVARLFGSNLKVKFDPEGEDKLLNELIGLYEKGLL"
     gene            9777..12818
                     /locus_tag="PK502_04900"
     CDS             9777..12818
                     /locus_tag="PK502_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPH00189.1"
                     /translation="MKKTLLTLVLITWGWVLSAQEITVTGTVISASDGIPLPGVSVTV
                     KANPSIGTATGIDGRYSIKVDANATLLFSFVGMETKEMPVNGQTIINVSMVEATQVLD
                     ELVVVGYGIQKKSLLTSSVAKVGSDEIAKTNPLRVEQALQGKTAGVQIISNSGQPGDG
                     LTVRIRGIGTNGTSDPIYLVDGMPVGGIDYLNPNDIESVQVLKDASATAIYGARGANG
                     VVLITTKSGSKGKMAVTYDYSFGLQNAWRKVSLLDASEYALIMNESYANDNAAIPFAD
                     VASLIDTVGSGTDWLDELFYKNAPVQNHQFSVSGGNDNSSFYSSFSYTTQDGIVAKGK
                     SNYERYTYRINSNHQYGKFGFGNNLSYSNKKNRGIGPNAEFGGPLAKAVNMDPLTPVK
                     YADGTWGVSSYAAQEVVNPVAFLSVLHSDYTENKLVGNVWGELELVKGLKVKSNFSID
                     YANGNSRTFTPVYDLGGNIKTLVSEASVTENRWFTWQNENTLSYSNQIDLHSFTAMVG
                     MTANKYHHEFVGGRKTDLLFDDFEHAWINNGTDEESYRAWGGADEHALLSYFARVNYN
                     FNEKYLLEAVLRADGSSNFGSNNRFGYFPAFSAGWILSKEDFLQGFTPLSFLKVRAGW
                     GRNGNEAIGAFKYTSIIGSGSKYTFGSDEVITVGSNPWGIPNPDLKWETSEQYNIGID
                     SRFFNERLNFTLDLYNKKTKDLLVVAPIPGYIGNAAPTVNGGTVTNKGVELELGYKTS
                     INKLDVDVSLSGAYNKNEVTDINNAEGKIYGAGLAVGMSNICMMQEGFPIAYFWGYKT
                     AGVFQNEAQILSYISSDSTVIQPNAKPGDLIWVDKNDDGKIDDNDRMLIGNPYPDFTA
                     GLNVNLAYRNFDFNMFWYGAFGQDIFDGTRRYDLPMSNWNASVLDRWTGEGTSNSNPR
                     VTIADPNKNYVNVSDFYVHNGSYLRLKNLTLGYTIPTTISQKVKIQRMRVFMSVSNLL
                     TFTSYKGFDPEIGAKSALDVGIDRNIYPQSRTFMFGVNLNF"
     gene            12838..14424
                     /locus_tag="PK502_04905"
     CDS             12838..14424
                     /locus_tag="PK502_04905"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPH00190.1"
                     /translation="MTKIKYFAVTLVLLLGMGSCSQDFLEMEPLTDRVEGNFYKTEKD
                     AFEALVSIYDVLQWQSSYGYHPWDLVSNVLSDDAFSGGSGPGDRPGLNRMGKFSSYTT
                     DEEALGLWKDRYSGIYRANIFLEKIDGIQFKDEALKDRYIAEARFLRGYFYFELVQFY
                     GHVPLILKTLTTSEYKQSQANPADVYNQIASDLLFAWEKLPGNISAAEYGRVSKWAAG
                     AMLARVYLFHKGYGKGVLGITSDLTVDNVPITESNIEQIVDDIINLSGHALVPNFADL
                     WGVNHENNKESIFEIQHTDKSNWGDWGWRNGTEGNWTVVMSGFRDVSDPIYDLGWGFQ
                     PASQSLANEFEPGDPRFSVSILDAAAENLVYKPQNCYQHTGYAFKKYYPKKADKPLSN
                     PDLNWGYNRVVIRLADVLLMGAELYLDNDLLKAQGYYEQVRKRAFGQSHVAPTLTNDQ
                     NGLDLIYHERRVEFAGEGIRYWDLLRRGLNYAQQKIDAASSASPYDETFKPATKGLLP
                     IPQSEISISGNILVQNEGYI"
     gene            14449..16221
                     /locus_tag="PK502_04910"
     CDS             14449..16221
                     /locus_tag="PK502_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPH00191.1"
                     /translation="MSKKNILFGVLVLVGLTWHACEPSEVGKPDVGKAPRVEDLSFTI
                     TPGEDAFHFVFTNTSSLKGIANWDLGNGSKAIGNTVTAYFPIAKTYTVTLTLYASGGS
                     ASLSQELTQNETDWDFLASPLITAITGGVESANGKTWVIDSINPGHLGVGPANETKPS
                     WWSAQPREKTGHFLYDDEFTFKLVDFVYGIDTHGKTHVNHDGNADEDGFAGGYYTSLL
                     WNDAYDADVATNDAARGALTWSLNEVNGKSYINISSQSGNISYDDGNPRSYEILEWND
                     NFLYLRSASAGNARYHKLIPKGYVKPKISYELSITPTLNPNEYSMQLSNVTIPADLTI
                     SKVEWDFGDASSPNVYTSTDYNEVVTFTYMAAGTYTVKVTVTDNNHDTYVVTSQVVVA
                     ADHPDYVPYIETGMSIYANFDDIFCKFAVDNADNVGSFSLIANPETGYKNDSKTCLQF
                     TKVNSQWANVYIKLSSAYRFDLTVQTKFKVLVYGNAGDKVLLKLENTDMGGNAWQTAT
                     HDLFYTIQESNEWEIAEFNFSGIGAGWDWTGTIFTSDVTTDPNFNTGFYNVVRIMVNP
                     GDATATYSVILDNWAGPTVNGWKK"
     gene            16324..>16917
                     /locus_tag="PK502_04915"
     CDS             16324..>16917
                     /locus_tag="PK502_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPH00192.1"
                     /translation="MLKRLLPILIAVALVGCKNQQLKQTEVGSGKFAVNKQVEAKVDS
                     LLSLMTLEEKIGQLNQLSGNGEVTGPISFPAEYMEALRKGLVGSMLNINGAAYTYKVQ
                     KVAVEESRLHIPLIFGYDVIHGYKTIFPIPLGEAASWDMEAIEKSARVAAIEASAAGQ
                     HWTFAPMVDIARDPRWGRVMEGAGEDPFFGSAVARARV"
BASE COUNT         4608 a         3522 c         3927 g         4860 t
ORIGIN      
        1 cagtgccgct tgggcattcc cgcgccacgt tccgcccccg acccatttgc gggtcatgct
       61 tttatgccgt tttacttata ccagcaactt tcaacgattc ccgcctttac gagcaccgct
      121 gtggatacct taccccagtt ggggctgaag acaaggataa aggataaagg ataaaaaaaa
      181 tcaagattca agattcaaga acttgtccgc ctgctggcgg aacttgtccg gctcctgacg
      241 gattccataa cttgtcattc acaacccttt aatcttttac tcttgcaggc ttgccttacc
      301 cggttcaagg tcagtttcca gtttttggaa actagccttg gcaaccatgg gcattggagt
      361 tgcttttttt acaggtaatc ctctataggc gcacaggtgc aaaccaagtt tctatctccg
      421 tatcccgagt caattttact tacgtaaggc caaaacttat tggaagcaat ccatgtcaag
      481 gggtatgcag ctttttgacg ggagtaggga tgatgccact cgtcggcaga cacctcggga
      541 gcggtatgag gagcgtttag caccaggttg tcggttgggg ttgcattttt acctgccttg
      601 atgtcctcca gctcagcaag gattgaaatc atagcctcaa taaacctgtc gagttcttgt
      661 ttcgattcgc tttctgttgg ttcaaccatc aagctctcgt gaacggggaa tgaaacggta
      721 ggcgcatgaa atccatagtc cataagcctg cgggctacat cacttgtatc aaccccgtat
      781 gttgttttaa agtgatggca gtcaaggatc atttcgtggg caacccgacc tgtttcaccg
      841 gtgtaaagaa tcttaaagtg cttttttagc ttggccgcca tgtagttggc gttaagaata
      901 gccattttgg ttacctgggt taacccctcg gcgccaagca tcaggatgta gccataggag
      961 ataggtagga tgctggcact accccaaggt gaagccgcta cagccgaaat accgttttct
     1021 ccaccggttt ttactacggg gtgggaggga agaaatttgg ttagatgttt ggctacagca
     1081 atagggccaa cgccagggcc gccaccacca tgaggtatgg caaaagtttt atgaaggttt
     1141 aggtggcaaa catcagctcc aatttctccg ggacttgtta gtccaacctg ggcattcatg
     1201 ttggctccat ccatgtaaac caagcctcca ttcttatgga ttatatctac catttcacgg
     1261 attttacttt caaaaacacc gtgagttgag gggtaggtta ccatgaaagc cgaaagagtt
     1321 tctcggtact gctcggcttt tgcttttaga tcgtccacat tgatattccc catctcatca
     1381 caagcaacta ccactacctg catacctgcc atagcggcac tagcagggtt ggtgccatgg
     1441 gctgatgatg ggattaatac tacatttcgc ttgctatcac catggcttag gtggtattct
     1501 ctgataacca ttagcccggt gtactcacca gctgctccgg aattgggttg aagtgatact
     1561 gaatcaaaac ctgtgatttc ggcgagtagg tgctccagct ccgaaatgat ttcgtggtag
     1621 ccggcagcct ggtcggctgg tacaaatgga tgtatattag ccatttccgg ccagcttaaa
     1681 ggaagcatgg ttgctgcagg gtttagcttc atggtgcagc ttcccagcga aatcatgcta
     1741 tggttaagcg caatatctct acgttcaagt tttttgatgt aacgcatcat ctcggtttcg
     1801 gattggtagc tgttgaatac cttctcggtc aggtagggtg acctgcgttg taagtttgca
     1861 ggtattccat tagcttcctc aacagatgaa atggggttgt gctcttttcc aatagccttg
     1921 gcaaacacat tcaacagggc gttgatgtcg ttaattgttg ttacctcatc aaagctaata
     1981 ccaattgatc cgttaggctt atagtttaga ttcagctcat tttccaaagc atttcgacgg
     2041 atatcctcca ggctaacacc tgctggtaaa gtaacttcga gggtgtcaaa aatattttta
     2101 atattttgtg tatagccaag cagttgcagc tgcttggcaa caagcatggc tacgctatgg
     2161 atatatgagg caattctacg aattccactt ggaccatggt aaactgcata cattccggcc
     2221 atcgtagcca gcagggcttg tgcggtgcaa atattggaag tggcacgttc ccgtttaatg
     2281 tgttgttccc tggtttggag agccattcga agcgctttat tcccgtgtcg gtcaatcgaa
     2341 acaccaataa tccggccggg catgctgcgt ttaaattcgt ccttgcaggc aaagtatgct
     2401 gcatgaggtc cgccaaaccc cattggtatt ccgaagcgtt gggttgagcc agctacaatg
     2461 tcagctcccc attcgcccgg aggggtaatt aaggccaggc ttagcaggtc ggccataacg
     2521 gtaaccaggg ttccggcctc gtgagctttt gctgtaaatg agctgtaatc acggatattc
     2581 cctttagccg aaggatactg aatcacaact ccaaactcac gtcctgtaaa gctatattcg
     2641 ctgtagcagc cggtaactac atcaattccc aacggttttg agcgcgtggt aataacggct
     2701 agcgtttgtg gaaaaatgtc ccgatcgaca aaaagtatat ttttcccggc cttaacagcc
     2761 tcccgtgatc ggaggttatg catcataatc atggcctctg ctgcggcagt tgcctcatca
     2821 agaagagagg cgttcgcaat ctccattcgg gtgaggtcga taaccatagt ttgaaagttg
     2881 agcagggcct ctagcctacc ctggctaatt tcagcctggt agggagtgta ggacgtgtac
     2941 catccagggt tttcaaatac gtttcgtaaa ataacagaag gggttacagt tccatagtac
     3001 cccataccga tatatgattt gtaaagcttg tttttagccg caattttctt cattcttccc
     3061 agaaactcgt attctgaaac agggttaggg aggtttaatg gctttctcag cctgatgttt
     3121 tgaggaacgg tttgatttat aagttcgtct aacgttgaaa cgccaatttc cttaagcatt
     3181 tcatcaattt catggtcgcg agggccatta tgacgtgcaa caaaattatc ggttgtcatt
     3241 gtgttgtgtt ttaattagag gatgtataaa agttgagcaa aattatattt tttaagtaca
     3301 caggctatgt gctgggtagg gctttaaaca tgttataaag cattactgaa aaatttaagt
     3361 cttgatgaaa gggttacttc tccgttcgtc tccaatggta gtgctttcgc catggccggg
     3421 gtaaacagcc acatcgtcgg ggaggaccat aagcttagtg gtaatgctcc tgataatggc
     3481 atcgtagctg ccgccaggaa ggtcggtacg gccaattgac cggtaaaaca gagtatcacc
     3541 ggttagtagc attttctcgt tttgaaggta aaggcaaata ctgccgggtg tatggcctgg
     3601 ggtatgaata atgccaattg aaccatctcc aagcagaagg gtattgccat cggccactat
     3661 tttatctatt tctggggttt tatcaaccga aaaaccaaag gacatggcct gttgcggaaa
     3721 ggttttgagt aagggtaaat catccgaatg ggcataggct tcaacattgt agatgctttt
     3781 taggaggttt aagcccagca ggtgatcaac gtggcagtgg gtattgacaa aatattttac
     3841 ctttaattca ttgtcgtcaa caaacctttt gattaaagca acctcggtgg cattatagca
     3901 accagcatca acaatggctg cattgtggtt ttcgtcagaa atcacgtatg tgttctcctg
     3961 aaaggggttg aagacaaaaa cttttacttt catcggcatt ggcaaattat gttataagta
     4021 ttcaaatcat taactttttt ttgaatttct tgcaacgagt ggatgagtcc aaaaggggca
     4081 tgctaccagc cagggaaaat gcttatgatt ggttggcttt agttttacta cagtgcaaaa
     4141 ctcgtttgga agcgttttcg atgtaaacaa aaacgtgtgg gaattatagc ggtgtgttgg
     4201 taaatggtta catgtgttgg taggcataga taggcttgtt acttgctttt ttccactcct
     4261 tttaacaacg gaacaaagat aaagtttccg tgggtagtct ttcttacttc ggtgctgcta
     4321 attttttcaa ctagcgtcat cacctgaccg ttttcatccc caaccggaat aaccattctt
     4381 ccaccaaccg ccagctggtc aacaagctca tcaggaatgg ttggcgcacc tgcggtaaca
     4441 ataattctgt cgaaagggct atagcccggt aatcccttat acccatcgcc aaaatgaaag
     4501 ctggggtgat agcccatttc ctccagcagc gatttggcgt taaggtatag ctctctttgc
     4561 ctttcgatgg tgaacagcct aacgcccagc tccataagga ttgcagcctg gtaacccgag
     4621 cctgttccta cctccagcac cttgttcatg tgttcaacct gaaggagctg ggtttggaac
     4681 gccacggtaa agggttgaga tatggtttgc ccatatccaa ttgggaaagc ctggtcgctg
     4741 taggcaaact ttaggaagga actttccata aaaaaatgac ggggaacccg gagcattgcc
     4801 ccaagcacct gcttatccgt aatccccttc gattcaattt cttcaattaa ccgtttccgt
     4861 agccctttat gcttataggt gtcctgctgc atcgcaactt ttatcaagtt aaaatggtcg
     4921 ttcaaatata catctaaaag ggcatttgaa aattttaaat gtaatagaca gcaaaagaga
     4981 tatttacact ttttttgcta tacctaattt ttttatgcca gcttgttgta tcatggtgtt
     5041 gctatttatt tgaaccattg tagttgataa gttacccgac taaaatgttg gagatgacgg
     5101 taaaatctat aagtttgcaa ctattcaccc attcaacata tgcttaaact tggttttttt
     5161 actgataata cgcagctggc tgaattggta ggagatttag ggagacaagc caatatagaa
     5221 gtggagggga tatgggcaga accccttcta aatggtgacg acaaacggaa tttttccaat
     5281 tgtttcagtg atcctgaaaa gctatatggc agtattgatg tgggagttat ttccatttca
     5341 tcatccgagc tctttatgga tatagttacg gcagccatta aaaatggagt gcatcctctg
     5401 gttgttggac tgcccgacct tcagctttct acgtataatc agctgagcct gcttagcgct
     5461 gagatggggg tggagcttgg ttttagccat atctaccacc ggtttagcgg gtttaattat
     5521 cccttagtca gcccaataat tgcccatctg acacgtggaa taaccatgaa ccagcttaat
     5581 caatttgaaa tggcggctac gcttaggttc gatatagcca ctattctttc gctaactaca
     5641 agtgaagtaa gaagaattag aacttatgtg ctgccccaat cgggtgaatg tccaagctgc
     5701 atgatggttt ttatcgattt ctttaacaac tcagcaatta cctataacct gacgttaggg
     5761 gctaaaagaa attttttgga acttcggctt aactcaattg gtgaggcaat tgaactttat
     5821 gaggatgaga taaaacttgc aacaagctcc cttggcggga tgatttcgac tagcattttt
     5881 ttagatgatt taaacaactt tacgggttgt attggcatcc aaagggagcc gttgctgggc
     5941 atttcatttg taatgaaaat acttagtgcg gttgaggcga tttctcaaaa aatggccagg
     6001 tagcgttact tatttctttc taaagaaatc ttgattaaca aagctaatat ttagtggttt
     6061 gacaaagaat taccttaaaa tctggtacat actgtttgat gtcgttggaa gcgcagctgc
     6121 ctggactctt ttctgtattt atcccgcaaa ggatgttggg ttggagtttt taagagcagg
     6181 ggcaacattt gagttcgatg ggaagttcta cgccagcctt gttttcttcg tactgttttg
     6241 gctagcgctg tttatagttg cagggttcta caataatttg tacagaaaat caagactcaa
     6301 ggaatttaca aaatcggtag gctatacgtt tgtgggtacc gttgtgattt tctttatact
     6361 tcttcttgac gacagcctgg caacatacaa tgacaacttt tccattattg gctccatgtt
     6421 cattttggaa ttaacattta cctatttccc aaggcttgtg cttacaacgg gaactattcg
     6481 gaaaattcat aggcgaatta taggcttccc aacgataatt gtaggtaatg ggtttaaggc
     6541 gctgcaagtt tacagggagc ttgaagccga aaagaaatct caaggtttta ggtttgttgg
     6601 ctacattgcc caggttaata accatgagga catgctgcaa agtcagcaaa aaaggttggg
     6661 ggagatggat tcaattgcag aagtagtgaa aaagcaaggt gttgaggagg ttattttggc
     6721 ggtggaggga gaagatgtct caacacttac ccgttgtatc aacgagcttt tgcccctgaa
     6781 agtgaattta agggctattc ccagcatgta cgactttttg cttggaaggg ttaagatgac
     6841 cgggattctg aacgcaccat taatcagcgt ttcgtttgac ctaatgccat tatggcaagc
     6901 caacattaag cgtatagctg attacacggt atcggcttta gccatagtcc tacttttgcc
     6961 ggtttctctt attcttggaa ttgcgataag gtgcacatcc caagggccta taatttttaa
     7021 gcaggagaga attggacagt acggccggcc tttttggata tacaagttca ggagcatgta
     7081 tgctgatgct gagaaatcag ggccgaccct ttcaggcaag gatgatccaa gggttacacc
     7141 tattggacgg tttatgcgga aaactaaact tgatgaaatt cccaacttta tcaatgtgtt
     7201 aaagggcgaa atgtcgttgg ttggcccacg tcccgaaagg cagtgttata tagaacagat
     7261 tgttcgcata gcaccacact acgttcacct tcagaaggta aaacccggta ttacctcatg
     7321 ggggcaggtg aaattcggct atgctaaaaa tgttgatgaa atggtggaga ggctgaagta
     7381 cgatatactg tatcttgaaa acatgtcgct ttttgtggac ctgaaaatta tcatttacac
     7441 cattttaacg atttttaaag ggaaaggact ttaaccctta agcagcaaaa agttcttaat
     7501 aggtttgtgt atagctcctt ttggcgtaag caggctttgg taaaaggtta cttcctcaat
     7561 ctccgattcc tgaaaggtgt agttttgcag cttttcggtc atatcataaa acgctctagg
     7621 atttcttata aagttaaccc gaccaagggt aatgtggggc ataaaaaggc ctttttccct
     7681 atcaaatcct aaggggagaa gcgctttgtc aattgtttgt ttaagattgt tcagagtttc
     7741 attttgctca atgccaaccc atataacccg gggtgtagga cgcccaaaac ttccaagctc
     7801 cttaacttta aggctgaatg agttgaagcc agtgagcgat atagaaagca ctttagaaac
     7861 gtcgcctaca agatcaaccg gtatttctcc cagaaaacgg agtgtgaggt gaagcatgcc
     7921 ctcgtcaacc cagttaatga aatcaaggtt atgctgctgc ttaagcttgt gccagcagtt
     7981 taaaagtttg gcttcaggct taatgccaac cgcaatgaag gttcgctcct ttttcaactt
     8041 tcatggagat ttatgtactt tccttgcaaa acttttaaaa agttgtaaat tgtagccaaa
     8101 aataaaaaaa attgactggg catggccaac tcaaaaaaat ttgctgtggt tctttcgggg
     8161 tgcggcgtac tagacggctc cgagattcac gaagcagtaa tgacgcttta cgctatcact
     8221 ttatcgggga gcagctatga aatttttgcc cctgatgtaa accagcatca tgtggtgaat
     8281 caccttaatg gaagcgaggt ggaccaaacc cgtaacgttc ttgttgaatc ggccagaatt
     8341 gcacgtggaa agattaaacc gcttggcgac tttagctccg atgtgtttga tgctattatt
     8401 ttccccggag ggtttggggt ggccaagaat ttatcgtcgt gggcttttga tggagcgggc
     8461 tgcagcgtga atgccgatgt ggctaatgcg gttcggtcta tgcacaggct gcataagcca
     8521 ataggagcgc tatgtatagc tccggttgta attgccagga tacttggagg agttacagta
     8581 acaataggaa aggatgctgc aacagtaaag gagatagaaa ccctgggtgc aagacatcag
     8641 atggccctgc aaacgcaggt tgtggtggat gaaaaaaaca tgattttttc tacaccatgc
     8701 tacatgctcg atgcaacaat tgtggatatt gccgaggggg ccaaggctat agtttccgaa
     8761 atgcttaaat ggatatagcg ggagaatata ttatagcaag cctttttcat aaagacctat
     8821 aagctcgttt agcagcttat cctcaccttc agggtcaaat ttaaccttaa ggtttgaacc
     8881 aaaaagtcgt gcaacaccct gccaaaatgt tgctgaaaat ccacccttca gctcttttac
     8941 taaatcgtag gaggttgcgc cggtttccct gtcgatgagc ttcatcagca tttttccttg
     9001 agaaacggtg aattttctca ggtccttctc atactgcatc cggagctcct tttcggcctg
     9061 gttgatgtat tccctgcgtt ctctttcggt cttaatcatt tttagatcct cgttcatctc
     9121 aagtagcttg tacttggcaa gctgggcgta ggggtaggta agctttaaat ttctaatcat
     9181 tttatagtac tggttcattt cgctccggct tttaaagcgc cgtttgccgt actttactac
     9241 ctctcgcaac ttgatgtagg gtattgtatc accctcgtag aaaacaatag tataaaggtt
     9301 aaccacaacc gaatctctaa cctgagcatg cgagtgaccc gatgacaagg tgattcccag
     9361 caggatagcc aatggtagta gcttactttt cgtcatgttt atccttgaac ttctcatttg
     9421 caaaagtaac attaaaacaa ccctttctac cgaatgttta ccttatgcga aacggcaaaa
     9481 atcaactttt atgccatagt tgagaatttg taaaaacaag cctgctcaaa ttataaaaat
     9541 tgcggcggca ttaaaccaaa ttttacagaa tacatttaaa ttataatagc ttaaaataca
     9601 gcattataaa tcttacctca ggcatagaga cgtacggttg acgtactgct ccgaaaacgt
     9661 tttcgacaga tgagaaactt ttgaaattat tatatagtgg ttatgatgtg ggtggttttt
     9721 tgacatgaac cagcaggatg taataccttg cctcctgtta accaaaaacc aaaccaatga
     9781 aaaaaacact actgacttta gttttgatca cttgggggtg ggttctgtca gcacaagaaa
     9841 ttacagttac gggaactgta atttcggcta gtgatggaat accactaccg ggtgtttcgg
     9901 ttaccgttaa ggccaatcct tcaattggaa cagccaccgg tattgatggg cgctacagca
     9961 tcaaggttga tgccaacgct acacttttgt tctcatttgt tggaatggaa acaaaagaaa
    10021 tgcctgtaaa cgggcaaaca ataattaatg tttccatggt tgaagctacc caggttctcg
    10081 atgagttggt tgtagttggg tatggcatcc aaaagaagag cctgctcacc tcttcggtag
    10141 ccaaggtggg aagtgatgaa attgccaaga caaatccctt gcgagttgaa caagctttac
    10201 agggaaaaac ggcaggggtt caaattattt ccaactccgg ccagcctggg gatggattaa
    10261 ctgtacgtat taggggtatt ggtactaatg gtacgtcaga ccctatttac ctggttgatg
    10321 gaatgccggt gggtgggata gactacctta atccaaacga tattgaatct gttcaggtgt
    10381 tgaaggatgc ttcggctacg gcaatttacg gtgcacgagg tgctaatgga gtagtgctta
    10441 taacaacaaa gagcggttca aagggaaaaa tggccgttac ctacgactat tctttcggtt
    10501 tacagaatgc atggcgtaaa gtttcgttac ttgacgccag cgagtacgcc ttgattatga
    10561 acgaaagtta tgccaacgat aatgctgcaa tcccttttgc tgatgtagca tcactgattg
    10621 atacggttgg tagtggaacc gactggctcg atgagctatt ctataaaaac gctcccgtgc
    10681 agaaccacca gttctcggta agcggtggca acgacaattc ttcgttctac tcatcctttt
    10741 cctatactac ccaggatggt attgttgcga aaggaaagtc gaactatgaa cgttatacct
    10801 accggattaa ctccaaccat cagtatggaa agtttggatt tggcaacaac ctttcataca
    10861 gcaataagaa aaacagaggg attggtccta acgccgaatt tggcgggcca ctggccaaag
    10921 cagttaatat ggacccgctt actccggtaa aatatgccga tggcacatgg ggagtgtcca
    10981 gctatgccgc ccaggaagtt gtaaacccgg tggcttttct aagcgttctt cacagcgatt
    11041 ataccgagaa caagctggta ggaaatgtgt ggggggagct ggagctagta aaaggcttaa
    11101 aggtgaagtc aaattttagt attgactatg ccaacgggaa tagccgcacg ttcacccctg
    11161 tttacgatct gggaggaaac attaagacac tggtttcgga ggctagcgta accgagaaca
    11221 ggtggtttac ctggcagaac gagaatacgc tgtcatacag caaccaaata gacctgcata
    11281 gctttaccgc catggttggg atgacagcaa ataagtatca tcatgaattt gtgggtggta
    11341 gaaaaaccga cctgctattt gatgattttg aacatgcctg gataaacaac ggcaccgatg
    11401 aggaaagcta cagggcctgg ggtggtgccg atgagcatgc cttgctttcc tactttgccc
    11461 gtgttaacta taactttaac gagaagtacc tcttggaggc tgttttacga gccgatgggt
    11521 catccaattt tggatcgaac aaccggtttg gttacttccc tgcattttca gccggctgga
    11581 tattatccaa ggaagacttc cttcagggat ttactccctt gtcattccta aaggttagag
    11641 ctggttgggg tcgaaatggg aatgaagcta ttggagcatt caagtacacc tcaataattg
    11701 gttcaggaag caagtacaca tttggttcag atgaggtaat aacggtaggt tccaatcctt
    11761 ggggcatacc caatcccgat ttaaaatggg aaacctccga gcagtacaac ataggtatcg
    11821 actcgcggtt ttttaacgag aggttaaact ttactctaga cttgtataac aagaaaacaa
    11881 aagacttact cgtagttgct cccattccag ggtatattgg taatgcagca ccaaccgtaa
    11941 atggtggaac agttaccaac aagggtgttg agctagagct gggttataaa accagcatca
    12001 acaagctgga tgtagacgta agccttagcg gcgcctacaa taaaaatgag gtaaccgata
    12061 taaataatgc cgagggtaaa atttatggag caggacttgc cgttggaatg agtaatatct
    12121 gcatgatgca ggagggattt ccaatagctt acttttgggg ttataaaact gccggggtct
    12181 ttcagaatga agctcaaatt cttagctaca tttcatccga tagcaccgtt attcaaccta
    12241 atgctaaacc tggcgacctt atttgggttg ataaaaatga tgacggtaaa attgacgata
    12301 atgaccgtat gttgattgga aatccatatc ccgattttac cgccgggtta aatgttaacc
    12361 tggcataccg gaacttcgat tttaacatgt tttggtacgg tgcttttggc caggatatat
    12421 tcgatggaac ccgccggtac gatctgccca tgtcaaactg gaatgctagt gttctcgacc
    12481 gttggacagg cgaaggaacc tctaattcaa atccaagggt taccattgcc gatcccaaca
    12541 aaaactatgt aaatgtttcc gacttttatg ttcataatgg ctcctacctt cgacttaaga
    12601 accttaccct gggctatact atacctacta ctattagcca aaaggtgaaa attcagcgga
    12661 tgagagtttt tatgtcggtt tccaaccttt taacctttac tagctataaa ggttttgacc
    12721 ctgaaattgg tgcaaaatct gcacttgatg ttggtattga ccgcaacatt taccctcaat
    12781 cacggacctt catgttcgga gtcaacctga atttctaaac cacaaaattt tatcaccatg
    12841 acaaaaatta aatactttgc tgttacccta gtgttacttc tcggaatggg ttcgtgctct
    12901 caggactttt tggagatgga gcctttaacc gacagggttg agggtaactt ttacaaaaca
    12961 gagaaggatg cctttgaggc attggtttct atatatgatg ttctccagtg gcagtcgagc
    13021 tacggttatc atccttggga tttagtttct aacgttcttt ccgatgatgc ttttagtggt
    13081 ggatctggcc ctggcgatag accaggtctt aatagaatgg gaaagttttc tagctatacc
    13141 accgatgagg aggcgcttgg tttatggaaa gacaggtact ccggtatata cagagccaat
    13201 atttttctcg aaaaaattga tggaatccaa ttcaaagatg aagcacttaa agatcgctac
    13261 attgccgaag cacgttttct taggggatac ttttactttg agctggttca gttctacggt
    13321 catgttccct taatccttaa aacccttacc acatctgagt ataagcaatc tcaggccaat
    13381 cctgctgatg tgtacaatca aattgcaagc gatctgctct ttgcatggga aaaattgcca
    13441 ggcaacattt cagctgctga gtatggtagg gtttcaaaat gggctgccgg agcaatgctc
    13501 gctcgagttt acctcttcca caagggatat ggaaaaggtg ttcttgggat tacttctgat
    13561 ttaactgttg acaatgtccc cataaccgaa tcgaatattg agcagattgt tgatgacata
    13621 attaatctaa gcggccatgc ccttgttcct aactttgccg atttgtgggg agtaaatcat
    13681 gaaaataaca aggagtccat attcgaaatt caacacaccg ataaaagcaa ctggggtgat
    13741 tggggctgga gaaatggaac tgaaggtaac tggaccgttg taatgtcggg atttcgtgat
    13801 gtatccgacc ctatttacga tttgggttgg ggctttcagc ctgcctccca gtctctggct
    13861 aatgaatttg aacctggcga tccacgcttc tccgttagca ttttggatgc agctgctgaa
    13921 aatctcgttt ataagcctca gaattgctac cagcataccg gctacgcctt taaaaagtac
    13981 tatccaaaga aagccgataa accgttgtcg aatcccgact taaactgggg ctacaacagg
    14041 gtggtaattc gccttgccga cgttctgcta atgggagctg agctatacct ggataacgat
    14101 cttcttaagg cacagggata ctacgaacag gttcggaagc gtgcttttgg ccagtcgcat
    14161 gttgccccaa ctttaaccaa cgaccagaac ggccttgacc ttatctacca tgaacgtagg
    14221 gtagagtttg ccggcgaagg cattagatac tgggatttac tacgccgtgg tttaaactat
    14281 gcacagcaaa aaattgatgc tgcaagcagt gcaagcccct acgacgaaac atttaaacct
    14341 gcaaccaagg gattactgcc cattccacag tcggaaatct ccatttcggg taatatcctg
    14401 gtgcagaatg aggggtacat ttaatttatt aacaataatc taatagctat gtcgaaaaaa
    14461 aatatactat tcggagttct ggttctagtt ggccttacgt ggcatgcctg cgaaccatct
    14521 gaagttggta aacctgatgt tggcaaagca cctagggttg aagacctgag ctttaccatt
    14581 acccccggcg aagatgcatt tcactttgtt tttaccaaca cctcttcgtt gaagggaatt
    14641 gcaaactggg atttgggaaa tggttctaag gctatcggga atactgttac tgcctatttc
    14701 cctattgcca aaacctacac agttacgctt accctgtacg cttcgggggg aagcgcctcg
    14761 ctttcgcagg agttgactca aaatgagaca gactgggact tccttgctag cccgctgatt
    14821 actgctataa cagggggcgt tgagtctgcc aacggcaaaa catgggttat tgatagcatt
    14881 aatcccggac atttaggtgt tgggccagct aatgaaacca agcctagctg gtggtcggct
    14941 caaccacgag agaaaacagg acacttcctc tacgatgatg agtttacatt taagttggtt
    15001 gattttgttt acggtatcga cactcatggt aaaacccatg taaaccacga tgggaatgct
    15061 gatgaggatg gatttgcggg gggatactac acctcattgc tttggaatga tgcctatgat
    15121 gctgacgttg ctactaatga tgcagcacgc ggtgctctta cctggtcatt aaatgaggta
    15181 aacggaaagt cttatattaa catttccagc caaagcggta acatttcgta tgatgatgga
    15241 aatcccagat cgtatgaaat tttagagtgg aatgacaact tcttatacct taggagtgca
    15301 agtgccggaa acgcacgcta ccacaaactt attcctaaag gttatgttaa acccaaaata
    15361 tcctacgagc tgagcattac tcccacgtta aatccaaatg agtatagcat gcagctttct
    15421 aatgttacca ttcccgctga cctaaccatt tctaaagttg aatgggattt tggcgatgct
    15481 tcctcaccca acgtgtacac ttcaactgac tataacgagg ttgtaacctt tacctacatg
    15541 gctgccggca cctatacggt taaggtaacc gttaccgata ataaccatga cacctatgtg
    15601 gttaccagtc aggttgttgt tgctgctgat caccccgact atgtgcccta tattgagacg
    15661 ggcatgtcca tctatgccaa ttttgacgat atattctgca agtttgctgt tgataatgcc
    15721 gacaatgtgg gttctttcag cttgattgct aaccccgaaa caggttacaa aaacgacagt
    15781 aagacctgcc ttcaatttac caaggtcaac agccaatggg caaatgttta cattaagctt
    15841 tccagcgcat acaggttcga tttaaccgtt caaaccaagt tcaaagtgct ggtttacggt
    15901 aatgctggcg acaaggtgtt gcttaagctc gagaacactg atatgggagg caatgcatgg
    15961 caaactgcca ctcatgattt gttttacacc attcaggaat ctaacgagtg ggaaattgcc
    16021 gaatttaact tttcgggcat tggcgccgga tgggattgga caggcacaat ctttacaagt
    16081 gatgttacta cggatcctaa ctttaatacg ggcttctaca acgttgttcg cattatggtt
    16141 aacccgggtg atgctactgc aacctatagc gttattctgg ataactgggc aggcccaaca
    16201 gtaaacgggt ggaaaaagta aatttttgaa atgcaccaga ggtaattcct ttgttggaat
    16261 tatctctggt ttgctccgac aacgcattcc acttcaatat ttttatacac taaaatgaaa
    16321 aagatgctta aaagattatt gccaattctt attgccgttg cccttgtagg atgcaaaaat
    16381 cagcagctga aacagacaga agttggtagt ggcaagtttg ccgttaataa gcaggttgag
    16441 gctaaggttg actccctgct tagccttatg acgcttgagg agaaaatcgg acagctaaat
    16501 cagctatctg gaaacggcga ggttactggc ccaatttctt tccctgccga atacatggaa
    16561 gcactccgaa agggactggt tggttcgatg ttgaatatca acggtgcagc atatacctac
    16621 aaggtgcaaa aggttgctgt tgaggagagc cggttacata ttcctttgat ttttggatac
    16681 gacgttatcc acggatacaa aacaattttc ccaattccgt tgggcgaggc tgcatcatgg
    16741 gatatggaag ctattgaaaa gtcggccaga gtagctgcaa ttgaagcgtc ggcggcaggg
    16801 cagcattgga catttgcacc aatggttgat attgcccgtg atcctcgttg gggtagggta
    16861 atggaaggtg ctggcgaaga ccccttcttc ggttctgccg ttgctagagc ccgggtt
//
LOCUS       DAOPHX010000045        16763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_3875_length_16763_cov_5.509576, whole genome shotgun sequence.
ACCESSION   DAOPHX010000045 DAOPHX010000000
VERSION     DAOPHX010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 16763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16763
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3875_length_16763_cov_5.509576"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(480..861)
                     /gene="rnpB"
                     /locus_tag="PK502_04920"
     ncRNA           complement(480..861)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK502_04920"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(877..1953)
                     /locus_tag="PK502_04925"
     CDS             complement(877..1953)
                     /locus_tag="PK502_04925"
                     /inference="COORDINATES: protein motif:HMM:NF021179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HPH00193.1"
                     /translation="MKVEVGDRVRFLNEVGGGKVLRILDKRMVLVETDDGFEIPVLAA
                     EVVVINRNADSQIIGDDSLPMPTLPKRTDKHPKESNQKAGSAAGISMQGATERQGDDI
                     MLLLGFVPDSLYTLASSGLKLFLINDSSYKIFYTLSSWNEGKLNPIISGLLEADTKEQ
                     VAYLVNDEARSAKTLNLQAIYHKGMPFKAYQPEFYDVEIASLKLQLAASYVENDYFDE
                     KACLVTIADTQKEVLLERMDRESIQGIIQEKENQHVKNKPKKSLPVNEIEEVDLHIEE
                     LVDSWSNLSPGEILNIQLARFETALQGGIKSNTKKMVFIHGVGNGKLKYEIHKLLDTR
                     FAGLKYQDASFKEYGFGATLVYLK"
     gene            2076..3284
                     /locus_tag="PK502_04930"
     CDS             2076..3284
                     /locus_tag="PK502_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HPH00194.1"
                     /translation="MMFTPGNISISDFEYSLPTDRIASHPLSKRDESKLLIYRDGEIG
                     SDVFKNIGNYLPEGSRLVLNNTKVVSARIIFLKETGATIEVFLLEPVFPADYSVSFSS
                     HEPVVWKCMVGNQKRWKTDTLERTVPQTDTKLKVRKSKKVSDGFEVEFSWTNTDLCFA
                     QVVELCGEVPIPPYLNRQSEEVDKYRYQTIYAQPEGSVAAPTAGLHFTPEVFSNLKKL
                     GITPDYITLHVGAGTFKPVKADVIINHEMHTEHFLADIDLIKGMSECDNFRVVVGTTT
                     LRTLESLYWLGVKASQNQLCPPYFISQWEPYKTEPIPFKAAFYELWRHMEANGLETLM
                     ASTQIIIAPGYNVQTADALVTNFHQPRSTLLLLVSAFIGEQWRDVYAYALRNDFRFLS
                     YGDSSLLFRS"
     gene            3288..5030
                     /locus_tag="PK502_04935"
     CDS             3288..5030
                     /locus_tag="PK502_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HPH00195.1"
                     /translation="MMNSVFLPTSLKEINALGWESADIILFTGDAYVDHPAFGAAVIG
                     RVLENEGYKVAIVPQPNWRDDLRDFRKLGRPKLFFAVTSGNMDSMVNHYTANLRLRST
                     DAYSAGGKAGFRPDYAITTYCNILKKLYPEVPIVIGGIEASLRRLTHYDYWSDTLKPS
                     ILIDSHADILVYGMGEQPIVEIARRIANGKAPSELKNIPQTAFFVANIPEVAADSSLI
                     ELHSYEDCIKSKKYFAENFKLFERESNKLVQKTIVEPVGDGFVVVNPPYPPPAVGEID
                     KWYALPYTRLPHPRYKGKDIPAWEMIKFSVNIHRGCFGGCSFCTISAHQGKFIASRSE
                     KSVLREVEQVIEMPGFKGFLSDIGGPSANMYMMGGIKREKCRECTRYSCIYPSICLNL
                     NASHSAANNLYIKIRSMHGVKKAFVSSGVRYDLFTGRNTQAEKDYFENLCRFHISGRL
                     KVAPEHTSEKVLKLMRKPSFRKFRQFKTEFDLINKKYALNQQLIPYFISSHPGSTEAD
                     MQELAREIKELNIRPEQVQDFTPTPMTLASVMYYTGINPYTGEKIYVARSRKEKLKQN
                     SYFFWYKAQNTKGS"
     gene            complement(5124..6302)
                     /locus_tag="PK502_04940"
     CDS             complement(5124..6302)
                     /locus_tag="PK502_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HPH00196.1"
                     /translation="MKTYNVKSYRTPLYRDAGFLLKDIPTAQKAFSDEQSLPITPELY
                     IYSRYRNPTVVAAEEYLASIEGSQWSLLCQSGMAAIDIALSVFQRANHKCKWAFFTEI
                     YGGTNSYIDSILVSRRGVDAVRFSPSGEAYSNVEFDSFMTTHKPDVVFFEVISNPMLI
                     VADAVEIIRIAKKYGAAVVVDNTFATPYLFKPLDFGADLVIHSATKYLSGHGNLSAGV
                     VSGNSPELARSAIEYRKLVGHMLSPDDAYRLHDMLKTFNLRINRHFENAATVAKFLSA
                     SSKVEKVIFPGMDYHPSHEVASKLFEGRGFGGIVTFDLKGASFEDKQKKCNEFINAVS
                     DSIPLIPTLGDADTILMPVEPVWGDKYPFPGMIRLSLGIENGQEITRVISQGLDAVGS
                     "
     gene            6399..6740
                     /locus_tag="PK502_04945"
     CDS             6399..6740
                     /locus_tag="PK502_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS sensor protein"
                     /protein_id="HPH00197.1"
                     /translation="MINGIDWAIGLGCAITVSAADGTILYMNEKSQKVFEKWGGKELI
                     GQRLWGCHSEKSVETIKRLIENKESNCYTIEKNGVKKLIYQTPWFDGEELGGLVEISI
                     ELPASIPHHVR"
     gene            complement(6787..7371)
                     /locus_tag="PK502_04950"
     CDS             complement(6787..7371)
                     /locus_tag="PK502_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HPH00198.1"
                     /translation="MTDFLDSNLYSYLVLPFLIFCARICDVTIGTLRIIFVSKGHKKV
                     APILGFFEVFIWVIAISQIMSNLNNFACYFGYAAGFATGNYIGMLVEERIAVGKLFIR
                     IITSADGTTLVKLLNSNGFGATTVKAEGGRGGVNMVYSVINRKRLDEIQKILLDFDPK
                     LFYTIEDVRKANAGIFPESFSSKIPLFSRWRRGK"
     gene            7563..9029
                     /locus_tag="PK502_04955"
     CDS             7563..9029
                     /locus_tag="PK502_04955"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPH00199.1"
                     /translation="MVVLLQPGETAFSQNLENFGAGIIKQEELIQTVGFLSSSRFHGR
                     LPGSMEYEQAARYVATRFKNAGIKPVGQPSMLQYFNDEVNLIHDASCFILNSNGRPII
                     PLTLGEDFVCRGFTGSGHVKGEAVFAGFGMKQPNYDDYRNINVKGKIVVAFKSAPPWT
                     SETGSWGDTSPRAKARIAKEQGAAGIIFIADPKVFPPTMIYGSIACGDGPHLFDFPMI
                     QLSDRATDSLFAELPVKAPNLYEQICQDKAPHSIELGRSVLVNVKAEYFAEKPTANVV
                     GIVEGSDSLLKNEYVVLGAHLDHVGYQGEKLYFPGANDNASGVAAIIAIAEALKKSKV
                     EIKRSIIFIAFSSEESGLRGSKYFVDHSPVDLSEVVAMLNFDCVGQGDSIAIGGRLSF
                     PKLWKKVKKIDKHTTKLLSTRTFGGGGADAEAFYRAGISTLYFNTTNGYKHLHSPSDR
                     AETINPELIEKVAKLGYLVTVDLANGRYKGERDRQRKK"
     gene            complement(9103..9642)
                     /locus_tag="PK502_04960"
     CDS             complement(9103..9642)
                     /locus_tag="PK502_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HPH00200.1"
                     /translation="MRILVFCVLMTVVGTAAGQTGSFYDFTVKDIDGNDFPMSSLKGK
                     KIMVVNTASKCGYTPQYADLQALYEKYQDQNFVVIGFPANNFMKQEPGSNKEIKEFCT
                     STYKVSFPMMGKISVKGKDMHPLYGWLTSKSLNGVMDSEVKWNFQKYLIDERGILVMV
                     IDPGEKPNSERVIKWIEGK"
     gene            complement(9708..10466)
                     /locus_tag="PK502_04965"
     CDS             complement(9708..10466)
                     /locus_tag="PK502_04965"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPH00201.1"
                     /translation="MKRLLNIVLLVCAPLWSLLAQNPSQLMKQANELYTSGKFKEAAQ
                     RYEAIIGQGYTSAEVYYNLGNAYFKMKDIPAAILNYERANLLSPKDEDIQFNLDLART
                     YVVDKIEPLPEFMLVTAAKNFRSWFSSNTWAYIAFFAFLLVMVAVAFYIFTNRYTIKR
                     LTFAGSILLTIVFVISITCSISLRNQSIQRNHAILFAPVVSVKSSPASTGSDIFILHA
                     GTKVMVTRSLGEWSEIKIADGSKGWVLSSTFERI"
     gene            complement(10481..12328)
                     /locus_tag="PK502_04970"
     CDS             complement(10481..12328)
                     /locus_tag="PK502_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPH00202.1"
                     /translation="MSKRFLLVAFIFVSSIIQAQNVQFEASAPNMVEVGEQFRLTYSL
                     NSKPSNFNPPTIAGFEVIAGPSTSSSTSIEIINGKVTQQQSVSYTYILEATAVGKYNI
                     PPAEAMVGKVTYQSNPVSIEVIKASSGSARQANQPQPTQPQQIAAGEENLPDDDLFVT
                     IELNRKTAYMGEPVEAAIKIYTRVNILGFEDAKFPTFDGFWSQELESSSNVQFERANV
                     NGKIYNVGIIRRYLLFPQKNEKLKIDPFELVVVYQGNAARPRSIFDEFFGGGFENYRK
                     RLVSKPVSVVVKSLPQGAPSSFYGGVGNFKINVGVDKNKLKTNEAVTLKLVVTGTGNL
                     KLMGTPKVEFPSTFEVFEPKVSDNINPSQQNASGSKTFEYVAIPRASGSYEIGPVDFT
                     YFDPSAEKYVSVQSKTVNLEVIPDSSATGAVVLTSGLSKEDVKFIGKDIRFIKTNPVR
                     LKPINSFVVGSLMYLFSYILVVILFAVFLFVFRKYRKQIQDTGFVRNKHAQKIAQKRL
                     QVAQEMLRLDEGEKFYEEIHKALWGYASDKLNLPVASLTTESVREALSGKGVSESAID
                     EFIRIIEACEYARFAPSSEHLQLETIYKDTYSLILQLEENLGKVKSRLW"
     gene            complement(12346..13041)
                     /locus_tag="PK502_04975"
     CDS             complement(12346..13041)
                     /locus_tag="PK502_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPH00203.1"
                     /translation="MKVFLMSTLMLTAVISTAQSERKFIRKGNHDYKKNDFVESEIQY
                     RNALEQNSHSFKGQFNLADALYKQNKFNESLETLNNLNLTGLSNDQKASVLHNKGNAL
                     FKQNKLKESIEAYKSALKFNPNDKETKYNLSEAIRLFQQQQQNQQDNNNKDKDKDKQN
                     QNQNQPDNPKDEQNNNPDNRDSQGNNQQQPKLSKEDAERILEAIQNHEMQIQDRLKEE
                     KAKQAKVRVGKNW"
     gene            complement(13038..14078)
                     /locus_tag="PK502_04980"
     CDS             complement(13038..14078)
                     /locus_tag="PK502_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPH00204.1"
                     /translation="MFRFAEPKYFYLLLVIPLMVGIYLYSIHRQKKQIAIFGRTDTVM
                     GLLPNLSFTRAWVKFIILLVACVFVVLSLARPQVGSKLTEVKRKGIEIIIALDVSNSM
                     LAEDIQPNRLERAKQSIAQLVDKLTNDRIGLIVFSGDAYVQLPVTSDFASAKMFLSSI
                     SPEMVSKQGTAIGRAIDLASNSFTPQGNTSKALIIISDGENHEDDPVESAKLAAEKGI
                     VIYTIGIGSPSGAPIPIGANQNYLKDENGNIVVSRLDEETLSKIAIEGNGKYIRASNS
                     QFGLLPLFENLSKMSKTEIKEKIYSEYSEQFQYFLAFALALLLIDSLILERKNPKLSR
                     FKLVDTKNGGRQ"
     gene            complement(14188..15171)
                     /locus_tag="PK502_04985"
     CDS             complement(14188..15171)
                     /locus_tag="PK502_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPH00205.1"
                     /translation="MVFAAPKMFYLLLLVPVLVAWYIFRQKDSRASLHISTSIGFENV
                     KPGFKVYMRHAPFILRMVAIILIITALARPQSTNIQKNISTEGIDIVLSMDVSGSMLA
                     RDFKPDRLGAAKEIGIKFISGRPNDRIGLVVFAGESFTLCPLTTDHATLINMFHNVNM
                     GILEDGTAIGSGLATAVTRLKDSHAKSRVIILLTDGVNNRGEIAPLTAAEIAKSFGIR
                     VYTVGVGTMGTAPYPVQTPFGTQYQNMKVEIDEDLLTSISKLTGGEYFRATNNTTLEE
                     VYGKIDKLEKSKIEVAEYRSREEKFRGFLLAAFAILVVEFTLRRTLLQGIQ"
     gene            complement(15182..16207)
                     /locus_tag="PK502_04990"
     CDS             complement(15182..16207)
                     /locus_tag="PK502_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00206.1"
                     /translation="MFRRFCLLVVSFLFIYVKSGFPQPQETEPSITSRLQRDTILIGD
                     QITFSIEVKSIKPLRVVFPNFTDSLGNGVEVVGIPTVDSLAKANYHHYSMNMLITAFD
                     SGTLVVPSIPVVLASGHTTDTLFTQPHALHVNLVPRDTTLKEIYDIKPPIAEPLTFAE
                     VAPLVGSGLLLAALVVLLILYVRSRKQNKPFLNLFKPKEPPHLVALRELEQLRNEKLW
                     ANENHKLFYTRLVDILRKYMEGRFGINAPEQTTSEILSELERIEYDFGHLKEELRELL
                     VISDLVKFAKHVPLIQENEKNMNFAFDFVNSTRPVETPEIPATTDDTGQQLQDAQSEN
                     QTTNKLS"
     gene            complement(16207..>16763)
                     /locus_tag="PK502_04995"
     CDS             complement(16207..>16763)
                     /locus_tag="PK502_04995"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPH00207.1"
                     /translation="SAILNNDKIGAILFSDKVEKFIPPQKGRRHILRIISELLNFKPE
                     GTGTNITEALKFLTNGIKKRCTAFLLSDFLDVERLSLKPLFADALTIAGNKHDLIGIR
                     IFDKRESDFPSVGLVKVRDNETGKYIWVDSNAPAVRSNFSNRWSQLSDNLKSTFTKAS
                     VDWVSIRTDEDYVKPLISLFKHRS"
BASE COUNT         4907 a         3527 c         3537 g         4792 t
ORIGIN      
        1 atatctggat agtagatatt tataatgtta ttttaactat tttactgttc aaaatacagc
       61 cacaatttat ttctttataa aatattagat taacatatat taataaacaa aagattatca
      121 gtttattata aaaccaaata aaatagcgtt gatatatcaa cttgcatgtc ctgtaaaatg
      181 agctttctgg cgttgttacg aatgatagat gttgccgcac gtaatttaat cagcgtagaa
      241 caaaatcaaa acgaaaaatt aaagtttata tgtgtaatca ctttaatttt aagtattatc
      301 taaaatgttg tttattagaa tattatttaa tatttagtta tgcttaaaga tcaaaaataa
      361 ctgtcctatt taaaaagttt taattatatt aaagttacat ctgtaacctt ataaatttat
      421 ataacaccat acaggccaat atcaggttaa cataattaaa actgttgggg agtaaaaaaa
      481 tagcaagtta tcctgtaagc cgggttctgt acgatgttaa tcgcctctac cattcatctg
      541 tgactgtagt tgcctacagc ctaaagcgac ctaccccttc cgataccacc tatgagtgga
      601 gcaaggcggg taaccctgtc tcttacgaga tcggaatata catggtcttg cagcccatga
      661 ggtgtacggc attccgtatt gctacgaaac ccggtgggct cttaccccac cttttcaccc
      721 ttaccctgcc agcagcagag cggttatttt ctgttacact actatgccct cacgagcatc
      781 ttcctgttag gaagcatggc accctttgct gcccggactt tcctctcctg tatttcaaga
      841 agcgatagag cggataactt gctatagaag attatactac tttaggtaaa ccaaggttgc
      901 accaaatcca tactctttaa atgaggcatc ctgatatttt aaaccagcaa acctggtgtc
      961 aagtagcttg tgaatttcgt acttcagctt gccgttacca acgccgtgaa tgaacaccat
     1021 ttttttggtg ttggacttaa tgcctccctg aagcgctgtt tcaaagcggg caagctgaat
     1081 attaagaatt tctcctggag aaaggttact ccagctatca accagctctt caatatgcaa
     1141 atcaacctct tcaatttcat tgaccggaag tgatttttta ggcttattct taacgtgctg
     1201 gttttccttc tcttgtatga ttccctgtat agattccctg tccattcttt ccaacagcac
     1261 ctctttttgg gtatctgcaa tagtaacaag acaggctttt tcatcgaaat aatcattttc
     1321 cacgtagctg gctgcaagtt gaagcttgag cgaggctatc tcaacatcgt agaattctgg
     1381 ctgataggct ttaaatggca ttcctttgtg gtaaatagcc tgcaagttca acgttttcgc
     1441 gcttctagcc tcatcgttta caaggtatgc aacctgttct ttagtatcgg cttcaagcaa
     1501 tcccgaaata attggattta gctttccttc attccatgat gataaggtgt aaaaaatctt
     1561 atagctgctg tcgttaatta gaaacaactt taagccactg cttgcaagcg tgtataggct
     1621 atcaggaaca aacccaagta aaagcattat atcatcaccc tgtctctccg ttgctccttg
     1681 catgcttatg cctgctgcac tacctgcctt ttgattgctt tctttaggat gcttatctgt
     1741 tcgtttggga agagtgggca ttggtaatga atcatcacca ataatctgac tgtcggcatt
     1801 ccggttaata acaacaactt ctgctgccag aaccggtatt tcaaacccgt catcagtttc
     1861 aaccaaaacc attcgcttat caagaatcct tagaacctta cccccgccaa cctcattcag
     1921 aaaacgcacc ctatctccaa cctcaacttt catactctat ttatttttaa actcatgatt
     1981 taacccaatc cattaaagtt aaaagaactg caaaattagc taattttgcc atttcaaggg
     2041 aatagggata caacagcgtg agtttgaatt ctaatgtgat gtttactcca ggcaatatta
     2101 gcatttccga ttttgagtat agcttaccaa ccgaccgaat agccagccac ccattaagta
     2161 aaagggatga gtcgaagttg ttgatatata gggatgggga aattggaagc gatgttttca
     2221 aaaatatagg caactacctt cctgaaggga gtcgtttagt tttaaataac accaaggtag
     2281 ttagtgcccg gataatcttc ctgaaagaaa ccggggctac aattgaggtt ttcttactag
     2341 aacctgtatt cccggcagat tattcggttt ccttttcaag ccacgaacca gtagtatgga
     2401 aatgcatggt tggaaatcaa aagcgatgga aaacagatac gctagagcga acagtacctc
     2461 aaactgacac taaattaaag gtgagaaaat cgaaaaaggt gtccgatgga tttgaggttg
     2521 agttcagctg gaccaacacc gacctatgct ttgcacaagt tgtagagctt tgtggtgaag
     2581 tgcccatacc cccctacctg aataggcaat cggaagaagt tgacaagtac cggtatcaaa
     2641 ccatatatgc ccaacccgaa gggtcagtgg cagcgccaac tgcagggttg cacttcactc
     2701 ccgaggtatt tagcaattta aaaaagttag gaattactcc tgattacata acgcttcacg
     2761 ttggtgcagg gacatttaag ccagttaagg ccgatgtaat aatcaaccac gaaatgcaca
     2821 ccgagcattt ccttgccgat attgacctca taaagggtat gtccgaatgc gacaatttta
     2881 gagtggtggt gggaacaaca acccttagaa cacttgaaag tctttattgg cttggcgtaa
     2941 aggcctccca aaaccagcta tgccctccat acttcatttc gcaatgggaa ccctataaaa
     3001 ctgaacctat tcccttcaaa gcagcttttt atgaactttg gcgccacatg gaggctaatg
     3061 gcctagagac cctgatggcc tcgacacaaa taattatagc accaggatat aacgttcaaa
     3121 cggcagacgc gcttgttacc aactttcacc agccccgttc gactcttcta ctgctggtat
     3181 cggcatttat tggtgagcaa tggcgtgatg tttatgccta tgcgctcagg aatgattttc
     3241 gttttcttag ctatggagac tccagcttac ttttcaggtc ataaaatatg atgaacagcg
     3301 tatttctacc tacaagccta aaggagataa atgctctggg ttgggagagc gctgatataa
     3361 ttttatttac aggagatgcc tacgttgacc atcctgcttt tggggcagcg gttattgggc
     3421 gggtgctgga gaacgaggga tacaaggtgg ccattgtgcc acagccaaac tggagagacg
     3481 atttaaggga tttccgcaag cttggcaggc caaaactttt ttttgccgtt acctcgggca
     3541 atatggattc gatggttaac cactatacgg ccaacctgag gcttaggagc acagatgcat
     3601 actcagcagg agggaaagca gggtttcgcc ccgactacgc cataacaacc tactgcaaca
     3661 tacttaagaa actctaccct gaagtgccta tcgtgattgg gggaattgaa gcatctttaa
     3721 ggcgactgac tcactacgat tactggtcgg acacgctgaa acccagcatt ttgattgata
     3781 gtcatgccga tatactagtg tacggaatgg gtgagcagcc aattgtggaa atagcccgac
     3841 ggatagctaa tggtaaagca ccatccgaac taaagaatat accccaaacc gcttttttcg
     3901 tcgctaatat tccagaagtt gctgcagata gtagcctcat cgaactacat tcctatgaag
     3961 attgcataaa gagtaaaaaa tattttgccg aaaattttaa actttttgaa agggaatcca
     4021 acaaacttgt tcagaaaacg atagtagagc ctgttggtga tggttttgtt gtggtaaacc
     4081 caccctatcc accgcctgct gtaggtgaaa tagacaagtg gtatgcatta ccctacaccc
     4141 gattaccaca tcccaggtat aagggaaaag atattccggc atgggaaatg ataaaatttt
     4201 cggtcaacat tcatcgcggg tgctttgggg gttgcagctt ttgcaccata tcagctcatc
     4261 aaggtaaatt cattgcttca aggtcggaaa aatctgtact acgagaggta gagcaggtaa
     4321 ttgaaatgcc tggctttaag ggattcctta gcgatattgg agggccttcg gcaaatatgt
     4381 acatgatggg tggaataaag cgagaaaaat gcagggagtg cactcgttac tcctgcatct
     4441 atccttccat ttgcttaaac ctaaatgcca gccatagcgc agcaaacaac ctgtacatta
     4501 aaattcgcag catgcatggg gttaagaagg cgtttgttag cagcggggta aggtatgatt
     4561 tgtttacagg cagaaacacc caggccgaaa aggattattt cgaaaacctt tgtcgttttc
     4621 acatctcggg taggttaaag gtagccccgg agcacacgtc cgaaaaagtt cttaaactga
     4681 tgagaaaacc cagcttcagg aagtttcggc aatttaagac cgaatttgac cttataaata
     4741 aaaaatatgc tttaaatcaa cagcttatac cctactttat ttcgagccat ccgggaagta
     4801 ccgaagcaga catgcaggag ttagccaggg agattaagga gctaaatata agacccgagc
     4861 aagtgcaaga tttcactcca acgccaatga ccctagcttc ggtaatgtac tacacaggaa
     4921 taaatcccta tactggtgaa aaaatttatg tggcccgaag ccgaaaggaa aagctcaagc
     4981 agaatagcta cttcttctgg tataaagccc aaaacacaaa aggaagctaa tacacaaaca
     5041 gggtggtcag tcttaaaaaa ttatcctgac aaaggttgat gtaacctcac caaaaggttg
     5101 gtcattagcc cataccaggg gaatcaactt ccaactgcat cgagcccttg cgaaatcacc
     5161 cgggtgattt cttgaccgtt ttcaatgccc aacgataacc taatcatacc ggggaatggg
     5221 tatttatcgc cccaaacagg ctcaacaggc atgaggatag tatcagcatc ccccagggtg
     5281 gggattaggg gtatgctatc cgaaacggca ttaataaact cattgcattt tttctgctta
     5341 tcttcaaacg aagcgccctt aagatcgaag gtaactatac caccaaatcc cctaccctca
     5401 aaaagtttgg aggcaacctc gtgcgaaggg tgataatcca ttcctgggaa tataaccttc
     5461 tcaaccttgc tgcttgcaga aagaaacttg gcaacggtag ccgcattctc aaaatggcga
     5521 ttaatccgaa ggttgaaggt tttcagcata tcatgaaggc ggtaggcatc atctggagaa
     5581 agcatgtggc caaccagctt acgatattca atcgctgacc tggctagttc cgggctattt
     5641 ccggaaacta cgcctgcaga aaggttacca tgaccagaaa ggtattttgt agcgctatga
     5701 attaccagat cggcaccaaa gtcgagtggt ttgaagaggt atggggttgc aaaagtatta
     5761 tcaacaacaa ctgcagctcc atatttcttt gctatgcgaa taatctcaac cgcatcggca
     5821 acgataagca tagggttgga aatgacctca aaaaatacca catcaggttt atgagtggtc
     5881 atgaacgaat cgaattctac atttgaatac gcttcgccac taggactaaa acgtactgcg
     5941 tcaacacccc ttctggaaac caggatgcta tcaatatatg aatttgtacc accataaatt
     6001 tcggtgaaaa atgcccattt gcatttatga tttgcccttt ggaaaaccga tagggcaata
     6061 tcgattgcag ccattcccga ctgacataaa agtgaccact gggagccttc gatagaagcc
     6121 aaatactcct cggcagcaac aacagtagga ttcctatagc gggagtaaat gtacagttcg
     6181 ggggtaattg ggagggattg ttcatcagaa aaggcttttt gagctgttgg aatatccttc
     6241 aaaagaaagc cggcatctct ataaagaggt gtacggtaag attttacatt gtatgttttc
     6301 attgcaggaa taatttattt atgctgcaag gtaaaaaatt ggtgggtaat ttgcgctgga
     6361 tgaaaagtaa taacttattt tgcccaaaaa tcaaggttat gatcaatggc atagactggg
     6421 cgattgggtt aggttgtgca ataactgtaa gcgcagccga tggaaccatt ctctacatga
     6481 atgagaaatc gcaaaaagtt tttgaaaagt ggggtggaaa ggaacttatt gggcaacgcc
     6541 tttgggggtg tcatagcgaa aagtcggtgg aaaccattaa acgtttaata gaaaacaagg
     6601 aaagtaattg ctatactatt gagaaaaacg gagtaaaaaa actaatctac cagacgcctt
     6661 ggtttgatgg agaggaactt ggaggattag tggaaatttc cattgagctg cccgccagca
     6721 ttccacatca cgtccgttaa taagtataca atcagcagga atgggggaat ccttttgctc
     6781 tagaaactac ttccctcttc tccagcggga aaataaagga attttggatg aaaaagactc
     6841 gggaaatatt ccggcatttg cctttcggac atcttctatt gtatagaaaa gcttgggatc
     6901 gaagtccaga aggattttct gaatttcatc gagccgcttc ctgttgataa ccgagtaaac
     6961 catattcacc cctcccctgc caccttctgc ctttactgta gtagctccaa agccatttga
     7021 attcagaagc ttaaccaggg ttgttccatc agcagaagta atgattctaa tgaaaagctt
     7081 tccaacggca attctttcct caaccagcat cccaatgtag ttgcctgtag cgaatcctgc
     7141 cgcatatcca aagtagcagg caaagttgtt aaggttactc attatctggc ttatggcaat
     7201 cacccatata aatacctcaa aaaagccaag aatgggagcg acttttttat gtcccttgga
     7261 gacaaatata attctaagtg tgccaatagt aacgtcgcaa attcttgcac agaaaatcag
     7321 aaatgggagt accaggtatg aatacaggtt tgagtcgaga aaatctgtca tattgataga
     7381 atgttttagt acaaaagtag ctgttaaaac caacagtttt tatatgcacg tgtattatac
     7441 tcctattttt atatctacct ttgaaaatgc ggtttaggta cttaaaatga agatattttg
     7501 aaaaggaaat caagatttta aggagttatt cgatgaatgc aggatttaaa atagcgattt
     7561 gcatggttgt tttgctacaa cctggcgaaa cagccttttc gcaaaatttg gagaattttg
     7621 gagctggcat tataaaacag gaagagctga ttcaaacggt tggcttcctt tcctcatcgc
     7681 ggttccatgg caggttaccc gggagcatgg agtacgagca ggctgcgaga tatgttgcaa
     7741 cccggtttaa aaatgcagga atcaaaccgg ttggacagcc aagcatgctg cagtacttta
     7801 atgatgaggt aaacctcata catgacgcta gctgctttat cctaaatagt aatggacgcc
     7861 ccattatccc gcttactctt ggtgaagact ttgtgtgccg gggttttaca ggatcgggtc
     7921 atgtgaaggg cgaagctgtt tttgccggat ttggcatgaa gcaacccaac tacgatgatt
     7981 accgcaacat aaacgtcaaa ggcaagatag tggtagcatt caaatcggct cctccttgga
     8041 cgtcagaaac gggcagctgg ggcgacacat ccccgagagc taaagcgaga atagctaaag
     8101 aacaaggagc agcgggaatc atttttatag ccgatccaaa ggtctttccg cctaccatga
     8161 tatatggaag catagcatgt ggcgatggcc cccacctgtt tgattttcca atgattcaac
     8221 ttagcgatag agcaacggac agcttgtttg ccgagctgcc agtaaaagcc cctaacttgt
     8281 atgagcaaat ttgtcaagat aaagctccgc actcaattga gctgggtaga agcgttttag
     8341 taaatgttaa agctgaatac tttgctgaaa aacccactgc caatgtagtt ggaattgttg
     8401 agggaagcga ttcgctgctt aaaaacgagt acgttgtgct gggagcacat ctcgatcatg
     8461 tggggtacca aggtgaaaag ctctactttc cgggtgcaaa tgataacgct tcaggagtag
     8521 cagccataat tgcaattgcc gaggctttaa aaaaatcgaa ggtggaaatt aaacgctcca
     8581 taatatttat tgccttttca tctgaagaat cagggttgcg cggttctaaa tattttgttg
     8641 accattcccc agttgacctt tcagaggtgg ttgccatgct taacttcgac tgtgttggac
     8701 agggcgatag cattgccatt ggaggaaggt tgagttttcc taaactgtgg aaaaaagtta
     8761 aaaaaataga caagcacaca acaaaactac tttcaaccag aacgtttgga gggggtggag
     8821 ccgatgcaga ggcattttac agggcgggta tctctacgct gtacttcaat actactaatg
     8881 ggtacaagca tctccattct ccaagcgaca gggctgaaac aataaatcct gaactgatag
     8941 aaaaagtagc aaaactagga tatttagtta cagttgattt ggcaaatggt aggtataaag
     9001 gtgaaagaga caggcaacga aagaagtaaa tccctattta tgaatctgta aaaaattcat
     9061 catccttctg tgtaaatcaa ctggcagacc ttcctgttat aattattttc cttcaatcca
     9121 tttgattacc ctttcagaat tgggtttttc tccaggatct ataaccatta ccaatatgcc
     9181 cctctcgtca ataaggtatt tctgaaaatt ccacttcacc tcgctatcca tcactccatt
     9241 caagctttta cttgtaagcc agccatataa gggatgcata tccttcccct taacagatat
     9301 tttgcccatc atcggaaagg aaactttgta ggttgaggtg caaaactcct ttatctcctt
     9361 atttgaacca ggctcctgct tcataaaatt attcgctgga aaaccaatca ctacgaagtt
     9421 ttgatcctga tacttttcgt ataacgcttg cagatcggca tactgtgggg tatagccaca
     9481 cttagaagca gtattgacta ccatgatttt tttgcccttt aggcttgaca tgggaaaatc
     9541 gttaccatca atatccttta cggtaaaatc gtaaaatgat cctgtttgtc cagctgcagt
     9601 gccaacaaca gtcatgagca cgcaaaaaac taatattctc atcggattgg aagttttagt
     9661 actatctgca tactaaaaca gctgcgtgca gaaaatgttc gaataagcta tatcctttca
     9721 aaggttgagc taagcaccca gcccttgctt ccatcggcaa ttttgatttc gctccattca
     9781 ccaagcgaac gggtaaccat tacctttgtt cctgcatgaa ggataaaaat atcactaccg
     9841 gttgaggctg gagaactttt aactgaaaca acaggggcaa atagaatagc atgatttctt
     9901 tgaatggact gattacgaag gctaatactg caggtaatgg aaataacaaa aacaatagta
     9961 agcagtatag atccggcaaa agttaagcgt ttgatggtgt acctgttggt aaatatataa
    10021 aacgcaaccg cgaccatcac taaaaggaat gcaaaaaaag caatataagc ccatgtatta
    10081 gaggaaaacc aacttctgaa atttttagcg gctgtcacca gcataaactc aggcaggggt
    10141 tcaatcttgt caactacata agtacgggct aaatcaaggt tgaactgtat atcctcgtct
    10201 ttaggactta aaaggttagc ccgttcatag ttaagaatgg cagcaggaat gtccttcatt
    10261 ttgaagtaag cgttgccaag gttgtagtaa acttcagcac tggtgtaacc ctgtccaatg
    10321 atagcttcgt agcgttgagc agcttcttta aatttcccag aggtgtaaag ctcgtttgcc
    10381 tgcttcataa gctggctggg attttgcgca agcaaactcc ataatggagc acaaaccagc
    10441 agcactatat taagtaatcg cttcatacgt tattaagatt ttaccaaagt cgggacttaa
    10501 cttttccgag attttcctcc agctgcaaaa taagcgagta ggtatcttta taaatggttt
    10561 ccaattgaag gtgctcggat gatggagcaa agcgagcata ctcacaagct tcaataatcc
    10621 taataaactc atcaattgca gattcactca cacctttacc ggaaagtgct tccctaacgc
    10681 tttcggtagt aagcgaggcg acgggtaaat taagcttatc ggatgcataa ccccataagg
    10741 ccttgtgaat ttcctcgtaa aatttttctc cttcatccaa cctgagcatt tcttgagcta
    10801 cctgcaacct tttctgagca attttctgag catgcttgtt tctaacaaaa ccagtatcct
    10861 gtatttgctt gcggtatttt ctgaatacaa acagaaaaac agcaaagagg attacaacca
    10921 aaatatagga gaacaggtac attagagaac ctacaacgaa ggagttaata ggctttaatc
    10981 ttacagggtt tgtcttaata aacctaatat ccttgcctat aaacttcaca tcttccttgc
    11041 ttaaaccact tgtaagcacc actgcccctg ttgccgagga gtcgggaata acttcaagat
    11101 ttacagtttt tgactgtact gatacatatt tttcggccga aggatcaaaa taggtaaaat
    11161 caactggccc tatctcatag ctacccgagg ctctagggat ggcaacatat tcaaaagttt
    11221 tactgcctga agcgttttgc tgggatggat tgatgttgtc tgaaaccttg ggttcaaaaa
    11281 cttcaaaggt agaaggaaac tcaaccttag gtgttcccat tagcttgagg ttaccggtgc
    11341 cagttacaac cagctttagg gtaacagcct cgttggtttt aagtttattt ttatcaacac
    11401 ccacattaat cttgaaattg cccacacccc cataaaacga ggagggtgca ccttgaggta
    11461 agctctttac tacaaccgat acaggtttgc taaccagccg tttgcggtaa ttctcaaagc
    11521 cgccaccaaa aaactcgtca aaaattgacc ggggtcttgc tgcgtttccc tgataaacta
    11581 caaccagctc aaagggatcg atttttaact tctcattttt ctgggggaaa agcaggtatc
    11641 gtcgtataat gccaacatta taaatcttcc cgtttacatt ggctcgctca aattgcacgt
    11701 tggaggaaga ttccaactcc tgactccaga acccatcgaa agtgggaaat ttagcatcct
    11761 caaaacccaa aatgttgacg cgggtatata ttttgatagc cgcttcaact ggctcgccca
    11821 tgtaggcagt ctttcgattc agctcaatag taacaaacag gtcatcatcg ggcaaattct
    11881 cctcaccagc ggcaatttgt tggggttgag taggttgtgg ttgattggct tgtcgcgcac
    11941 tgccggatga tgctttaatt acctctatgc ttacagggtt agattggtag gtaacctttc
    12001 caaccattgc ctcagcgggt ggtatgttgt actttcccac agcggtggct tccagaatat
    12061 aggtgtaaga aacgctttgc tgctgggtaa cctttccgtt tattatttca atacttgtgc
    12121 tgctagaggt gcttggccca gcaataactt caaagccagc aatagtagga ggattaaaat
    12181 tcgaaggttt tgaatttaaa gagtatgtca agcggaattg ctcaccaact tcaaccatat
    12241 tgggagcaga agcctcaaac tggacattct gggcttggat gatgcttgat acaaaaataa
    12301 atgcaactag tagaaatctt ttgctcatat tcaacttcgg ttagcctacc aattttttcc
    12361 aactcgaacc tttgcttgtt ttgctttttc ttccttcaag cgatcctgta tttgcatttc
    12421 gtggttttga atggcctcca aaatgcgttc ggcatcctct tttgaaagtt tgggctgctg
    12481 ctggttattc ccctgagaat ccctgttatc ggggttgtta ttctgttcat ccttcgggtt
    12541 atctggctga ttctggtttt gattttgttt atccttatcc ttatccttgt tgttattatc
    12601 ctgctggttc tgctgttgct gctggaacaa ccttattgcc tccgaaaggt tatacttggt
    12661 ttccttgtcg ttggggttaa atttcagggc acttttatag gcctcaatgc tctcctttag
    12721 cttattctgt ttgaaaagtg cgtttccctt attatgaagt accgatgctt tctggtcgtt
    12781 tgataagccg gttaaattca aattattgag ggtctcaagc gattcgttaa acttgttctg
    12841 cttatagagc gcatcagcca ggttaaactg gcccttaaag gaatgagagt tttgttccag
    12901 cgcgttgcgg tactgaattt cggattccac gaaatcattt tttttataat cgtggttacc
    12961 cttgcgtatg aacttgcgct ctgactgggc agtagaaatt acagctgtta gcattaatgt
    13021 ggacatcaag aatactttca ttgcctgcct ccatttttgg tgtcaactag cttaaaacgg
    13081 gatagctttg gatttttcct ttcgagaatc aaggaatcaa taagcagcag tgccaaggcg
    13141 aatgctaaga aatattggaa ttgctcgctg tactccgagt atattttttc tttaatttcg
    13201 gtcttgctca tcttgcttag gttttcaaat agaggcagca gcccaaattg tgagttcgat
    13261 gctctaatgt acttaccatt tccctcgatg gcaattttgg aaagggtttc ctcatcgagt
    13321 cttgagacta cgatgtttcc attttcgtcc ttaaggtagt tctgatttgc gccaataggg
    13381 ataggcgctc ccgaaggcga gcctatgcca atagtatata taacaatacc tttctctgca
    13441 gcgagcttgg cagactcaac agggtcatcc tcatgatttt cgccatccga aataattatg
    13501 agcgccttac tggtgttacc ctgaggggtg aacgagttgg aggcaagatc aattgcccgc
    13561 ccaatggcgg tgccttgttt tgataccatc tcgggactta tagaggaaag aaacatctta
    13621 gctgaggcga aatcgctggt tacggggagt tgaacatagg catcgcctga gaaaactatc
    13681 aaccctatac gatcgttagt cagcttgtca acaagttggg caattgactg ctttgctctt
    13741 tccagcctgt taggttgaat atcctccgcc agcatgctgt ttgaaacatc gagggcgata
    13801 ataatttcga tgcccttacg ctttacctca gtcagctttg atcctacttg cgggcgtgct
    13861 aacgatagaa caacgaacac gcaagcaacc agcaaaatga taaattttac ccatgcccta
    13921 gtaaaggaaa ggttgggaag taaacccatc actgtgtctg tcctaccaaa aattgcaatt
    13981 tgtttttttt gacggtgtat ggagtagagg tatataccca ccatcaaagg tatcactaga
    14041 agcaagtaaa agtattttgg ttctgcaaac ctgaacatag ctcaacaatc aatttgttgt
    14101 taacaaaagg ggcaaaggca tatttttgca atgtaacagt ttacctatgt gtttgtaact
    14161 acttctcatg aatggctcaa ttagcattta ctgaattcct tgcagtaaag ttctgcgtaa
    14221 tgtaaactca acaactagta tggcaaaagc agccaataaa aatccacgaa atttttcctc
    14281 cctgcttcta tactcagcca cctcaatttt agatttttcg agcttatcaa ttttaccata
    14341 cacctcctca agggttgtgt tgttagtggc acggaaatat tcacctcccg ttagctttga
    14401 gatgctggtt aacagatcct catctatttc aaccttcatg ttctggtact gtgtaccaaa
    14461 aggggtttgc accgggtatg gtgcagttcc catggtgcct actccaaccg tgtaaaccct
    14521 aataccaaaa cttttggcta tttcggcagc tgtaagaggg gcaatttcgc ccctgttgtt
    14581 aaccccatcg gtaagtaaaa taattaccct gcttttagca tggctatcct tcaaccgcgt
    14641 aactgctgta gccaaaccag agcctatggc tgttccatcc tccagaatgc ccatgttaac
    14701 attatgaaac atgttaatca gggtggcatg gtcggtagta agcggacaaa gggtaaagct
    14761 ctcgccggcg aaaactacca gtccgatcct gtcattaggc cttccactaa taaattttat
    14821 acctatttcc tttgccgctc caagcctgtc gggcttaaaa tcacgagcca gcatgctgcc
    14881 cgagacatcc atggaaagga caatatcaat accctcagta gagatgttct tttgtatgtt
    14941 agttgattga ggccttgcca atgccgtgat gatcagaata attgcaacca ttctaagaat
    15001 aaagggagca tgccgcatgt aaaccttaaa gccgggcttt acattctcaa agcctattga
    15061 agttgaaata tgcaggctgg ccctggaatc tttctgacga aagatgtacc atgcaaccaa
    15121 aaccggcacc aaaaggagca ggtaaaacat cttgggggct gcaaaaacaa tttccgacat
    15181 atcaactcaa tttattagta gtttgatttt cggattgagc atcttgaagt tgctgtcccg
    15241 tgtcatccgt tgttgcagga atttcgggcg tttcaacggg tctggtgcta ttcacaaaat
    15301 cgaatgcaaa gttcatattc ttttcatttt cctgaattag aggaacatgc ttggcaaact
    15361 ttaccaaatc ggaaattacc agtaactcac gcagttcttc ctttaaatgg ccaaaatcat
    15421 actcaattcg ttcaagttcg cttaaaattt ctgaggtggt ttgttcggga gcatttatac
    15481 caaacctacc ctccatgtac ttacgaagaa tatctactag cctggtgtag aaaagtttat
    15541 gattttcatt tgcccaaagt ttttcgtttc gcagctgttc gagttctctg agcgcaacca
    15601 ggtgaggggg ttctttaggt ttgaatagat tcaggaatgg tttattctgc ttacgacttc
    15661 taacgtataa aatcagcaaa acaactagag cagccagtag taaaccagaa ccaaccagcg
    15721 gggcaacctc ggcgaatgtc aaaggttcag caatgggtgg cttgatatca taaatttcct
    15781 taagtgtagt atcgcgagga accaggttta catgtagcgc atgaggctgg gtaaaaagtg
    15841 tgtcggttgt gtggcccgat gccaacacta ccgggattga ggggacaacg agagtgccac
    15901 tatcaaatgc agttatgagc atgttcatgc tatagtggtg gtaattagct ttggcgagcg
    15961 agtcgactgt tgggatgcca accacctcaa ccccgttgcc aagggaatcg gtgaagttcg
    16021 ggaagacaac ccgtaggggc tttatgcttt taacctcaat gctaaaggta atttgatcgc
    16081 caattaaaat tgtatccctt tgaagccgcg aggtgatgga tggttcagtc tcctgcggtt
    16141 gagggaaacc actttttaca taaatgaaaa gaaaagaaac aaccagtaaa caaaatctcc
    16201 taaacattag cttctatgtt taaaaaggga aattaaaggt tttacgtaat cctcatcagt
    16261 tcttatagaa acccaatcaa cactagcctt agtaaatgtt gatttaagat tgtcggaaag
    16321 ttgactccat cggttgctaa aattgcttct tacagctgga gcgtttgagt caacccagat
    16381 gtacttgccg gtttcgttgt ccctcacttt gactaatcca actgaaggga aatcgctttc
    16441 gcgcttatcg aatatgcgaa tgccaattaa atcgtgtttg ttacctgcga tggtaagagc
    16501 gtcggcaaac aaaggtttta gggaaagcct ttcaacatca agaaagtcgc tcagtagaaa
    16561 ggcggtacac cgttttttga tcccgttggt aaggaattta agggcttcgg taatattcgt
    16621 gccggtgccc tcgggtttaa agttaagcag ctcgctgatt attcgcagga tatgccttct
    16681 gcccttttgg ggtggaataa acttttcaac tttatcgctg aaaagaatag cgccaatttt
    16741 atcattatta agaattgcag aaa
//
LOCUS       DAOPHX010000046        16457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_3970_length_16457_cov_6.157176, whole genome shotgun sequence.
ACCESSION   DAOPHX010000046 DAOPHX010000000
VERSION     DAOPHX010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 16457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16457
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3970_length_16457_cov_6.157176"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1133
                     /gene="porQ"
                     /locus_tag="PK502_05000"
     CDS             84..1133
                     /gene="porQ"
                     /locus_tag="PK502_05000"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HPH00208.1"
                     /translation="MFQKCFITVLVLLSSLCGFSQVGGFGAYQFLNLPFSVRSAALGG
                     KVATIDEPDMAMVMLNPALLDSTLHNNLYLSYSRYVDDISYGAFAYGRSSRWGTFGVN
                     IQQVSYGSFIEADETGLVQGTFKANELAVSLMYARTLDSLFSVGISVKPVYSHLERYT
                     SFGLAVDIGGNYHSRNGLFSSGVVLKNIGLMLKGYTRGNPETLPFEIVAGVSQKLEHA
                     PFRFLVTFHQLQNMNIYYESKLQDSETSLGENSSQPGFAERAGREFLSHLIIGMEFVP
                     VKSFYLRAGYNYQRRNELKVPEKVSMVGFSWGFGVKVAKLHISYSRATYHLAGSTNQF
                     SICTNLNNFVSTKPI"
     gene            1234..1923
                     /gene="cmk"
                     /locus_tag="PK502_05005"
     CDS             1234..1923
                     /gene="cmk"
                     /locus_tag="PK502_05005"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPH00209.1"
                     /translation="MGKKITIAIDGFSSCGKSTFAKKIAERLGYLYIDSGAMYRAVTL
                     EFLNWEAFRNNTPDTSMTQQILSGLQVGFSFNEQKKTYETTLNGKVVEDAIRSPRISE
                     LVSAVSAIPEVREKMVELQRLMGKDKGVVMDGRDIGTVVFPDAELKIFMTADPAIRAE
                     RRYKELQSKGVQASLEEVLNNILERDKLDQTRAISPLRKAEDAIELDNSYMTIDEQME
                     WAEKLIAARIC"
     gene            2061..2942
                     /locus_tag="PK502_05010"
     CDS             2061..2942
                     /locus_tag="PK502_05010"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPH00210.1"
                     /translation="MQVDIDQKSGFCFGVVNAINQAEVGLKQNEEFYCLGSIVHNEQE
                     ISRLEKKGLRVVSRDEFSGLSGKKVLVRAHGEPPSTYELAKENDVELIDATCPVVLKL
                     QERVRKAWEQMKIAGGTVIIYGKKGHAEVIGLVGQTDGNAVVVESPNDLTGIDFSKPV
                     EIFSQTTKDAEMFKQIVDVIKEKMQSCFLSSNIPIKVNHTFCGQVANRKKELIGFAAT
                     HDVIIFVGGQTSSNGKMLFQTCRSSNPKSYYVSSANELRPEWFAGANSVGVSGATSTP
                     LWLMEQVAQEIERITMP"
     gene            3067..3429
                     /locus_tag="PK502_05015"
     CDS             3067..3429
                     /locus_tag="PK502_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00211.1"
                     /translation="MKREVRIVGTIFLVALYCFAASVVCIPKLVPLEEPHNGPGYFFN
                     ASSNLLSLTLPSKNPAGSIGLSPIWVLKNQLKDFSYTIKSVELFLSTIFTQYRNVSIS
                     YPIRLRKTDLLYPFQYFW"
     gene            3545..4045
                     /locus_tag="PK502_05020"
     CDS             3545..4045
                     /locus_tag="PK502_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00212.1"
                     /translation="MNTGIAIIGVLIVVISIIPFILVSRAKRKKEKRLRSEIAGMAKS
                     HGCSISMLDVLKDYAVGADENKHFIFFYKHQNGNETKLAINLADIQSCRVINSTRTIG
                     GRDSQYVITDKVALCLTPVEKSNPEIHLELYSADDGTQLVGELELVEKWSKAINNQLK
                     SMKPIK"
     gene            4513..6402
                     /gene="ispG"
                     /locus_tag="PK502_05025"
     CDS             4513..6402
                     /gene="ispG"
                     /locus_tag="PK502_05025"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HPH00213.1"
                     /translation="MNYCKNLYSYSRRETQEVNIGGVPIGGSNPIRIQTMTTTNTNDI
                     LATVEQCIKAAKAGADYVRITTQGVKEATSLSSIKQELLNRGFTTPIIADIHFNPNAA
                     MEAAKYADKVRINPGNFVTSHAKPTGGSGEQQEEADNTALIEAKLVPLLELCRERGVA
                     LRIGVNHGSLSERIMAKYGDTPEGMVESALEFIKICARHNFRDIVVSMKASNVRVMVQ
                     AVRLLAARMLNEGMEYPIHLGVTEAGNGEDGRIKSAVGIGTLLADGIGDTVRVSLTED
                     PENEIPVAKKLVSYFENRSSFGRIPSMDPLPYNPFSYTRRKTIAVGSIGADNPPVVVA
                     NLSLATKITGKLLSAWGWTYNHAANQWEHSELAADYFYIGSTTIDEEVITDGLPLLST
                     ANSSSQILRQGNKPASFNISAQPICQMVTDADVLSGHLFSTVMVSPNTVLILRTGYSN
                     GFAGQRAAFIRLMNNHENIPVIISREYDDDDRESFQLKASADIGGLLVDGLGDGVMVT
                     APAIADEDICSTVFSVLQAARTRITKTDYIACPGCGRTLFNLQQTLEKVKAHTGHLRG
                     LKIAVMGCIVNGPGEMADADYGYVGAGPGRVTLYKGKQVMKKNIPEEDALDELIGLIN
                     ADRGL"
     gene            complement(6603..8027)
                     /locus_tag="PK502_05030"
     CDS             complement(6603..8027)
                     /locus_tag="PK502_05030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPH00214.1"
                     /translation="MQQQKVSRGLVLALVMVSSFITPFIGASVNLALPLIGKDFGLNA
                     VGMSWVTMAFLLSSAVFLVPIGKIADIAGRKRVFFWGNIIMAISSVLCAISPSGAVLI
                     TFRAVQGLGSAMVFATGVALITSVFPPSDRGKAIGLNVTSVYIGLSAAPLIGGLLISW
                     FGWESLFYVPAFLGIFSALSIRMAVRSEWADARGENFDFAGAAIYVPSMTLFMYGFSQ
                     LPDPVAVCLTILGFIGLIFFVRVELRASYPVFNVRLFRNNRLFAMSNVAALINYATTF
                     AITFVLSLYLQYIKGLSPREAGLLLIVQPLLMAFVSSWSGKMSDKHDPRILASLGMGI
                     IAVGLALLIPIGKNTSITYLIVAQAILGFGFGLFSSPNTNSVMGSVEKKFLGIASGTV
                     ATMRLTGQMASMGIATLILQLYIGKSDINPGNYRQFGWSVGTTFTVFVLLCIVGVWAS
                     LARGKNNGNSMRELNPENEMERIA"
     gene            8138..9481
                     /locus_tag="PK502_05035"
     CDS             8138..9481
                     /locus_tag="PK502_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPH00215.1"
                     /translation="MAKILVVDDDSTFCLMLKTFLTKHEHSVVEAFSFDEAVRQIRSE
                     NFDVVLTDFRLPEKSGMDVLSEAKHKSSATVVIMMTGYADIRMAVNAIKHGAYDYVTK
                     PINPDEILTSISRALPGNRKAIDKDGTHNFIFINGNSEHSQEVSRYISIVAPTNLSVI
                     IQGDSGTGKEYIARKIHAESKRSSKAFVPIDCGALPRELAASEFFGHLKGSFTGAIAD
                     KIGQFEVANGGTLFLDEIGNLPYDIQVNLLRAIQERKIKRIGSSKEIPVDVRIIVASN
                     ENLLNMVNSGAFREDLYHRLNEFTIRVAPLHERKDDLLIFAKHFLSLANVELGKGING
                     FDADVLDILMDYTWPGNLRELKNVIRRAVLMEQSNLLTVSSLPDEIVHRSGPSDDDKS
                     AKHVENLRNIKEKAEYDLIISTLEKAKYNKSKAALLLNIDRKTLYNKMKHYGISG"
     gene            complement(9548..9949)
                     /locus_tag="PK502_05040"
     CDS             complement(9548..9949)
                     /locus_tag="PK502_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00216.1"
                     /translation="MMERDYILRVIQEFFKLINGLLGKLNEDNADEIHKQLQEVYSAY
                     FDRSCQFFYRLSCDEMITLIDNDDQFRHVEKVEMLSELLYRDGLFTSNDVLRKDLLCK
                     ALALIERIEKQSKSFSVERNDRKVGILAMLN"
     assembly_gap    10091..10190
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10191..11022)
                     /locus_tag="PK502_05045"
     CDS             complement(<10191..11022)
                     /locus_tag="PK502_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00217.1"
                     /translation="MKSKANILALLLAMMATTAIAQDSFIVTRIKGNVVNYRTGTSLR
                     AGDVLQPSDKVTFDNFDSYVISIGQNMGRYMIKLHEPPAPDAIQQLTAIVKDVAVPTK
                     RRSLMAERYRPNESVVEDLGSYFGNDKFSVIGNEVLVPVNPDKFQLNDSRFIVFYYRV
                     NNNPVSKKIGYEQNVLKIEKDKLLNTSAGTISNDEIPNVAVYRYESNTHSSEEITRFN
                     LIFVDKEQLTNEFLTILPILHRQNMSKDDIKKYLIEYYYDFYGVTDSKSIKKFVDDLV
                     D"
     gene            complement(11141..11464)
                     /locus_tag="PK502_05050"
     CDS             complement(11141..11464)
                     /locus_tag="PK502_05050"
                     /inference="COORDINATES: protein motif:HMM:NF016152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HPH00218.1"
                     /translation="MNVEEIRDYCLSKPGATESLPFDDETLVLKVGGRIFAMISLSGD
                     SSLTLKCDPEIALELQEKYTFVEPGYHMNKKFWITLRDTTLIPRNLVMQWIDSSFVLV
                     AAGKK"
     gene            complement(11469..11849)
                     /locus_tag="PK502_05055"
     CDS             complement(11469..11849)
                     /locus_tag="PK502_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPH00219.1"
                     /translation="MGQIVNTDKRKDLGTLGEDSAAEYLVSKGYKILHRNWHFGHKEL
                     DIVARDGNMLVVVEVKTRTSDYWEEPKEAVRRKKQKRLVEAADAYVCHYNLDTEVRFN
                     IVSVVIANGSLAIEHIEDAFYPTL"
     gene            11954..12631
                     /locus_tag="PK502_05060"
     CDS             11954..12631
                     /locus_tag="PK502_05060"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HPH00220.1"
                     /translation="MIRLKEQKWGHFRRFILVIPMLLLFPFNLLSNIDNFEGKIKFLS
                     EGVYDTLSMVLSVKNDLVRMDQYSGKNGLEISYLFDLRSGKVFALSGREKVYKEIYLH
                     DQSKKRRETDVTIIKSQNSMLINGTTCYQWRVKNRQSMTEVTYWVAQSGFDFMEPLQR
                     LIGQLQTPFNLFTLCPDIKYFFPLMVVDRTIFRKFRCSLRVVDIKRVVLPDNLFAIPR
                     GYRELLG"
     gene            complement(12597..13547)
                     /locus_tag="PK502_05065"
     CDS             complement(12597..13547)
                     /locus_tag="PK502_05065"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPH00221.1"
                     /translation="MENITPPYLKPGDTVGIIAPARFVSHEDVNPAIRFLTARGFIVK
                     TAPSLFAKHFQFAGSDSERASDFMEMVNDSNVKAILCARGGYGTVRILDRINLRKLQL
                     KPKWIVGYSDITVLHSIITSWFGIETLHATMPVNFPKNINDSESASSMVDVLMGRNPA
                     YSIPHHPLNRKGTATGFLTGGNLSILASLIGTDADTSTQERILFIEDVDEYLYHVDRM
                     MLQLKRAGKLKSIAGLVVGHFSEMKDNNIPFGLDAYQIISEAVKEYRIPVCFGFPAGH
                     QEPNLPLIMGRKVQLVVNSQGASLTFHESSSQAILDTHGE"
     gene            complement(13793..14902)
                     /locus_tag="PK502_05070"
     CDS             complement(13793..14902)
                     /locus_tag="PK502_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPH00222.1"
                     /translation="MNKFTRLIALVAIGLILCSWGQTGHYIISSKSSLSFNLEMEQFQ
                     TWASYLAEHASDADKRKDEDPNEAPKHYIDIENYPEFINAGYIPQTLNEAITLHGSYN
                     VYDWGILPYATKATFDSLKSNMARLNWDRAKFFAADLGHYVADGHMPMHITKNYNGQL
                     TGNDGIHSRYESTMVNAREQQLSQYEGAEIHQIDNVTQYILNYLYANYKYVDSVLAAD
                     NYATGISSNYSSTAYKNALWEKTGEFTTMLFANASHALAELMYTAWKEAGSPSLTGVD
                     MIGQDAEGYYLYPIRPNPARNEVSISFHLEKPCVANLIVCDGNGKIIATLAQGELGGG
                     NQTITWLTRNQPNGIYLVALRANGRQFSQKLVVNH"
     gene            15488..16000
                     /locus_tag="PK502_05075"
     CDS             15488..16000
                     /locus_tag="PK502_05075"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HPH00223.1"
                     /translation="MGINTPSPIGYIEEKQFGIIRRSYYVCAYQPYRATITGILGSND
                     TDRETIIREFTRFTFTKLHQQGVLHKDYSGGNILVEDLGEDSYRFSIIDLNRMNFKKK
                     VSTKQGLKNLRRLTLDITVNDIVTITSEYAALAGNSNPKQTLFWFLKYHLDFVRFKNF
                     KSWYKKFKRG"
BASE COUNT         4669 a         3730 c         3588 g         4370 t
ORIGIN      
        1 ctttggttac gtctgtgctt ttgaagcatg gaggtttcat attaatttga tttgtttact
       61 tttacgagga aaaatcataa tccatgttcc agaagtgttt cataacagta ttggttttgc
      121 tcagcagtct ttgtgggttt agccaggttg gaggatttgg agcctaccag ttcttgaacc
      181 ttccattctc ggttcgttct gctgcactgg gaggcaaggt ggcaactatt gacgaaccgg
      241 acatggccat ggtgatgctt aaccctgccc tgctggattc aacgcttcac aataaccttt
      301 acctttccta ctcacgctac gttgacgata taagctatgg tgcatttgcc tatggcagga
      361 gcagcagatg ggggacattt ggggttaaca tccagcaggt tagctatgga tcattcatag
      421 aggccgatga aaccggattg gttcagggaa catttaaggc caacgagtta gccgtgtcgc
      481 taatgtatgc caggacactc gatagccttt tcagtgttgg catcagcgta aaaccggtat
      541 attcgcatct ggagcgttac acctcatttg ggcttgcagt ggatattggt ggcaactacc
      601 actcgcgtaa cgggctgttt tcgtcgggag tagtgttgaa aaatattggg ctaatgctta
      661 agggttatac ccggggtaac ccggaaacct tgccttttga aattgtagcc ggagtaagcc
      721 aaaagctgga gcatgccccc ttccgttttc tggttacatt ccatcagctg caaaacatga
      781 acatctacta cgaatcaaag ctgcaggata gtgaaacaag cctgggcgaa aatagctcac
      841 aaccaggatt tgccgaaagg gcaggaaggg aatttcttag ccatttgata atcggaatgg
      901 agtttgttcc ggttaaaagc ttttacctaa gggcgggata caactaccag cgccgaaatg
      961 agctgaaggt gcccgagaaa gtatccatgg ttggcttcag ctggggattt ggagtaaaag
     1021 ttgcaaaact tcatatcagc tacagccgtg ccacctacca tttggcagga tcaaccaacc
     1081 agttctcaat atgcaccaac ctcaacaatt ttgttagcac aaagccaatt tagccgaata
     1141 gacttaactc tttttttata ataactttgc tccggatttt ctagcagtcc taggctgctt
     1201 ccgcaacaca tctatactaa cgcgataatc tggatgggta aaaaaataac aattgccatt
     1261 gatggcttct cgtcgtgcgg gaaaagcacc tttgcaaaaa aaattgccga gcgtttggga
     1321 tacctctaca ttgacagtgg agcaatgtac cgggcagtaa ccctagaatt tcttaattgg
     1381 gaagcattcc gcaacaacac acccgacacc agcatgaccc aacaaatcct gtcgggatta
     1441 caggtgggat ttagcttcaa cgagcagaaa aaaacctacg aaaccaccct aaacggaaag
     1501 gtggtggagg atgccattcg cagcccacgc atttcggagt tggttagcgc cgtatcggcc
     1561 attcccgagg taagggaaaa gatggttgag ctgcaacgcc ttatgggcaa ggataaaggc
     1621 gttgtaatgg atggtcgtga tatcggaacc gtggtgtttc ccgatgccga gttgaagatt
     1681 tttatgaccg ccgatcctgc aatcagggca gaacgtcggt ataaagagct gcaatcaaag
     1741 ggagtacagg ccagcttgga ggaggtgctt aataacatat tggaaagaga caagcttgac
     1801 caaacccgcg ccatttctcc actccggaag gctgaagacg ccatagaact tgacaatagc
     1861 tacatgacca tagacgagca gatggagtgg gccgaaaagt tgattgccgc aagaatctgc
     1921 tgaaccaaac tcccgaaagg ttgttacata tttggtatga ggttgacggt tgccggcaag
     1981 caaaaagaat gttcactatg cgcaaaccgg atttaaatcc ataagcgcta ctttaaaatc
     2041 agcattgaat tggtacctcc atgcaagttg atatagatca gaaatcggga ttttgctttg
     2101 gcgttgtaaa cgccataaac caagctgaag tcgggcttaa gcagaatgag gagttttact
     2161 gcctgggcag catcgttcac aacgagcagg aaatttcacg gctcgaaaaa aaagggctaa
     2221 gggttgtatc ccgagacgag ttctccggcc tttcgggtaa aaaggtgctg gtgcgtgccc
     2281 acggcgaacc accgtcaacc tacgagctgg caaaagaaaa cgacgttgag ctcattgatg
     2341 ctacctgtcc ggttgttctc aagctgcagg aacgtgtaag gaaagcgtgg gagcaaatga
     2401 aaatagccgg cggtacggta attatctatg ggaaaaaagg ccatgccgag gttatcgggc
     2461 tagtgggcca aaccgatggg aatgccgtag tggtggaatc gccaaacgat ttaaccggaa
     2521 ttgatttctc caaaccggta gagatattct cgcagaccac taaggatgcg gaaatgttta
     2581 agcagatagt ggacgtaatt aaggaaaaaa tgcaatcctg ctttctgtcc tcaaacatac
     2641 ccataaaggt taaccatacg ttttgcggac aggtagccaa ccgaaaaaag gagctaatcg
     2701 gatttgccgc aactcacgat gttatcatct ttgttggagg tcaaacgagc tccaatggta
     2761 aaatgctttt tcaaacctgt aggtcaagca atccaaaatc atactatgtt tcctccgcca
     2821 acgagcttcg gcccgaatgg tttgctggag caaacagcgt gggcgttagc ggtgcaacat
     2881 ctactccctt atggctgatg gagcaggtgg cacaggaaat tgaacgcatc accatgccct
     2941 aaattcctca tattctgcct accattcctt caagtaaatc atatggcaga taattttccg
     3001 ctcaataacc tacaaaagag cttattatga tgctctggca ataatttttt tgcttacgtt
     3061 tgcaacatga aaagggaagt tcgcatagtc ggcaccattt ttcttgttgc tctttactgc
     3121 tttgcagcaa gtgtggtttg catccctaaa cttgttcccc ttgaagagcc acataatggg
     3181 ccaggctact tttttaacgc ctcatcaaat ctgctttccc taactctacc ttcaaaaaat
     3241 ccggcaggca gcattggcct tagccccatc tgggttttaa aaaatcagct aaaagacttt
     3301 tcctatacta taaaatccgt tgagctgttc ctgtccacca tatttacaca gtaccgaaat
     3361 gtttccatta gctacccaat aaggctccga aaaaccgatc tgctttaccc atttcaatac
     3421 ttctggtaag cgcattccaa cttaaccaca ttgccgcttt cggcaactcc taaaggtttt
     3481 ttgcaggtat tgtgcctcaa caaaacctga ataactattt ttcaacttat ctaaatctac
     3541 aaaaatgaat acaggaatag ctattatagg tgttttaata gtggttatca gcatcatacc
     3601 atttattctc gtaagcaggg cgaaaaggaa aaaggaaaaa aggctgcgca gtgagattgc
     3661 cgggatggcc aaaagccatg gctgcagcat ttccatgcta gatgttctga aagactatgc
     3721 tgttggagct gacgaaaaca aacatttcat attcttctac aagcaccaga atggaaatga
     3781 aaccaagctg gctattaacc tggccgacat tcagagctgc cgggtaataa attcaacgcg
     3841 cacaattggt ggaagggaca gccagtacgt cattaccgac aaggtggctt tatgtcttac
     3901 tccggtggag aaatccaacc ccgagattca tcttgaactc tatagtgccg acgacggcac
     3961 ccagctggta ggagagctgg agctggttga aaaatggtcg aaagctatca acaaccagct
     4021 taaaagcatg aaacccatca agtaaaccaa taacttcttt acaccttacc attgaaaagc
     4081 ccttcatgca cgattgaagg gcttttttcg acctacctca accacttttt aaattgacct
     4141 tgaaatgagc ttatctaatg tttactgaat cagcgttagg gttattttct caaaactcat
     4201 caatctactt gtaattatcc gttgaaaatc atgcaattag gatggacatt tttctatggc
     4261 accactagac ttgcgatatt tagcccattg tgggtattgg tttctttacc taaccatttt
     4321 caatagaatc ttaaatcagc atgcaccttg gctggttacg tgccgattat acgaactacg
     4381 gaattatgca aatatgaaac tggaataaat tttcacagta aaaaaatatt cggattttac
     4441 ttacttgcat aagccatttg ttaccgaagt ttgttaattt gtaaacgaaa aacaacagcc
     4501 agatagaagc aaatgaacta ctgtaaaaat ctctatagct actccaggcg tgaaactcag
     4561 gaagttaata ttggcggggt gccaataggt ggttcgaatc ccattcgtat tcaaaccatg
     4621 accaccacca acaccaacga catcttggca accgttgagc agtgcattaa agccgccaag
     4681 gcaggcgctg attatgtcag gattacaact cagggggtta aggaagccac cagccttagc
     4741 agcataaagc aggaactgct aaaccgtggt ttcacaaccc ctataattgc cgatatacac
     4801 ttcaacccaa atgcggcaat ggaggcggcc aagtatgccg acaaggttag gataaacccg
     4861 ggtaactttg ttacttccca tgcaaagcca actggaggga gtggtgaaca gcaagaagaa
     4921 gcagacaata ctgccttgat agaagctaag ctggtcccgt tgttggagct ttgccgagaa
     4981 cgaggtgttg cactaagaat aggggtcaac catggttctc tatccgaaag aattatggcc
     5041 aagtacggtg atacccctga aggaatggtg gaatcagcct tggagttcat caaaatatgc
     5101 gccagacaca attttcgaga catagtggtt tccatgaagg caagcaacgt gagggtcatg
     5161 gttcaggccg taaggttact ggcagccagg atgctcaacg aggggatgga gtaccccatt
     5221 caccttggcg taaccgaggc cggtaatggt gaagatggcc gtattaaatc ggctgttggc
     5281 attgggaccc ttcttgccga tggaattggc gacaccgtta gagtatcgct taccgaggat
     5341 cctgaaaacg agattcccgt ggctaaaaag cttgtttcct acttcgaaaa tcgatcctcc
     5401 tttggcagaa ttcccagcat ggaccccctc ccctacaacc cattcagcta cacccgaagg
     5461 aaaaccatag ctgttggtag catcggggcc gataatcctc ccgttgtagt ggccaacctc
     5521 tcgttagcca caaaaataac cggtaaactt ttatcggcat ggggatggac atacaaccat
     5581 gcagcaaacc aatgggaaca ttcagaactt gctgccgact acttctacat tggctcaaca
     5641 accattgacg aagaagtcat tactgatggc ttaccgttac tttccactgc caactctagt
     5701 agccaaatac ttcgtcaggg aaacaaaccc gcctccttta acatttcggc gcaacccata
     5761 tgccaaatgg ttaccgatgc cgatgtgctt tcaggccacc tattctccac tgttatggtt
     5821 agtcccaata ctgtattaat tctgagaact ggctactcca atggttttgc aggacaaagg
     5881 gcagctttca taaggttaat gaacaaccat gagaacattc cggtgataat tagtcgggag
     5941 tacgatgatg acgataggga atcctttcaa cttaaagcct cagcagatat cggcggactg
     6001 ctggttgatg ggttgggcga tggggttatg gttaccgcac cggcaattgc cgatgaggat
     6061 atttgctcaa ccgttttttc cgtgcttcaa gctgcccgta cacgaataac caaaaccgat
     6121 tatatagcct gccctggttg tggacgaact ctcttcaacc tgcagcaaac gctcgaaaaa
     6181 gtaaaggctc acaccggcca ccttagggga cttaaaattg cagtaatggg ctgcatagtg
     6241 aatggaccgg gcgaaatggc cgatgccgac tatggatatg ttggagctgg tcccggtaga
     6301 gttacactct acaagggtaa acaggttatg aagaaaaata tacccgagga ggatgccctt
     6361 gatgagctaa tcggcctgat taatgccgac aggggtttgt aacttttttc ggctttactc
     6421 aaccccaggg caactcacaa atggccagat tttcttgttt aagcccattc gggttttgta
     6481 cggctttgta cgagaagcta taagcctaag taagctgttg aaattgccaa atatcatggg
     6541 aaaagcaacc gtgaatgggg tatcgaaaac acccaatcat ccgggtaaaa ctcaaaccat
     6601 tcctaggcta ttctttccat ctcgttttcg ggatttaact cccgcatgga gttgccgttg
     6661 tttttccccc tggcaagcga tgcccacacc ccaacaatgc atagcaaaac aaaaactgta
     6721 aaggttgtcc ccaccgacca gccaaactgc ctgtagtttc ctgggttgat gtccgacttg
     6781 ccaatgtaaa gttgaagtat aagggtggca atgcccatgc ttgccatctg gccggttaac
     6841 ctcatggtgg caactgtccc cgaagcaatt cccagaaact tcttttctac agaacccata
     6901 acagagttgg tgttgggtga tgaaaaaaga ccaaagccga atcccagtat ggcttgtgca
     6961 acaattaggt aggtaatgct ggtattctta ccaattggaa ttagcaaggc cagccctacg
     7021 gctattatgc ccattcctaa tgatgccaga attcgcggat cgtgcttatc ggacattttt
     7081 ccagaccacg acgatacaaa tgccattagt agcggctgaa caattagcag caatcccgcc
     7141 tcgcgaggag atagaccctt aatatactga aggtaaaggc tgagcacaaa tgtaatggca
     7201 aatgtggttg catagttgat tagtgcggct acatttgaca tggcaaacaa gcggttgttc
     7261 cgaaaaagtc tgacgttgaa tacagggtag ctagcccgaa gctcaactct aacaaagaaa
     7321 ataagcccga taaagcctag aatggtcagg caaactgcaa cagggtccgg aagttgtgaa
     7381 aagccataca tgaaaagggt catggaggga acgtagattg cagcccccgc aaagtcgaaa
     7441 ttctcacccc tggcatcagc ccactccgat cgaactgcca tccttatgga aagcgctgaa
     7501 aaaattccca gaaaagccgg tacataaaat aaactttccc atccaaacca ggaaattagt
     7561 aagccaccaa ttaggggtgc cgccgatagc ccaatataca ccgaggtaac attcaaccca
     7621 atagcctttc cacggtccga tgggggaaaa accgaggtaa ttaatgccac ccctgtagca
     7681 aaaaccatgg cgcttcctaa cccctgaaca gccctaaaag ttatgagcac ggcacctgat
     7741 ggggagatag cgcacagcac cgaactaatt gccatgatta tattgcccca aaaaaataca
     7801 cgttttcgcc ccgcaatatc ggctatctta cctatcggaa caagaaacac cgcagaggag
     7861 agcaaaaatg ccatagtaac ccagctcatc cccactgcgt tcaaaccaaa atccttaccg
     7921 ataagcggta gagccagatt aaccgaagca ccaataaacg gagtaataaa cgacgatacc
     7981 ataaccaacg ccagaactaa acccctacta accttctgtt gctgcatctt aaaaaatttt
     8041 cgcaaaggta ttcgttttag tactcttcaa agtagtgttt attttttttt agctgaaaaa
     8101 ccagctattt tagcgttgga ttagatcttc acgacttatg gctaagattc tggtagtgga
     8161 cgatgactca acattctgcc tgatgctcaa gacctttcta accaagcatg agcatagcgt
     8221 ggtggaagca ttttcgtttg acgaggctgt taggcaaatt cgctcggaga attttgatgt
     8281 cgtcctgacc gacttccgcc ttccagagaa aagcggaatg gatgtgcttt ccgaggccaa
     8341 gcataaatca tccgccacag ttgtaatcat gatgaccggg tatgccgata ttcgcatggc
     8401 ggttaacgcc ataaagcacg gtgcttacga ctatgttacc aaacctataa atcccgatga
     8461 gattcttacc tccattagca gggctctacc gggcaacagg aaagccattg acaaggacgg
     8521 cactcacaac tttatattta taaatggcaa tagcgagcat agccaggagg taagccggta
     8581 tataagcatt gtggctccaa caaacctttc cgttattatt cagggtgata gcggaaccgg
     8641 aaaggagtac atagcacgta agattcatgc cgagagcaag cgtagcagca aagcttttgt
     8701 tccgattgac tgcggggctc tcccgcgcga gcttgccgcc agcgagtttt ttggacacct
     8761 gaaaggctcc ttcaccggcg ccattgccga taaaataggt cagtttgagg tagccaatgg
     8821 tggaaccctt ttcctcgacg aaatcggaaa cctaccctac gacatccagg ttaatcttct
     8881 tagggccatc caggaacgaa aaattaagcg tattgggagc agcaaggaaa tccccgtgga
     8941 tgttagaata attgttgcct ccaacgaaaa ccttctaaat atggtgaact ccggagcatt
     9001 cagggaagac ttgtaccacc ggttaaacga gttcaccatt cgggttgccc ccctgcatga
     9061 gcgaaaagac gacctgctaa tttttgccaa gcattttctt agccttgcca acgttgagct
     9121 gggaaaaggt atcaacgggt ttgacgccga tgttctggat attctaatgg actacacctg
     9181 gccaggaaat ctaagggagc ttaagaatgt gattcgtagg gctgtgctga tggagcagtc
     9241 gaacttgcta accgtttcaa gtcttcccga cgaaatcgta catcgttccg gtcctagtga
     9301 tgatgataaa tcggccaagc acgttgaaaa cctccgtaac ataaaggaaa aagccgagta
     9361 tgatttgatt atatcaaccc tcgaaaaggc taagtacaac aagagcaagg ctgccctatt
     9421 gctaaatatt gaccgtaaaa ccctgtacaa caagatgaag cattacggaa tttctggcta
     9481 aaaaaatgat atagtaaaaa atgctgccat tcagtctgcc tcctgttggc acatttcaac
     9541 attctttcta gttcagcatg gccagtattc ccactttcct atcgtttcgt tcaaccgaaa
     9601 aagacttact ttgcttttca atccgctcaa ttagggcaag cgccttacat agcagatcct
     9661 tccgtaaaac gtcattgcta gtaaacaacc catcgcggta aagaagctcc gaaagcattt
     9721 caaccttttc aacgtgtcgg aactggtcat cattatcaat aagggttatc atttcatcac
     9781 aacttagccg atagaagaac tgacaggacc tgtcaaagta agcgctgtaa acttcctgaa
     9841 gctgcttatg aatctcatca gcattatcct cgttaagctt accaagcaag ccgtttataa
     9901 gcttgaaaaa ttcctgaata accctaagaa tgtaatcccg ctccatcata aggcctacag
     9961 cttatcccaa agattttaag gtaaaggtaa aataaaaaat agtagtatct cccccaacgt
    10021 attggaggtt ttacaccata cataataaga tggaataggt gtgacaggct gatgcaaagg
    10081 attcacaagc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn catcaaccaa
    10201 atcatctaca aattttttaa ttgatttgga atcggttaca ccgtaaaagt cgtagtagta
    10261 ctcaatgaga tactttttaa tgtcatcctt gctcatattc tgccgatgaa ggatgggcag
    10321 aattgtaaga aactcgttgg taagctgctc cttatcaaca aaaatcaggt taaatctggt
    10381 aatttcctcg ctggaatggg tgttactttc atacctgtaa acggcaacat ttggaatttc
    10441 gtcattggaa atagttcctg cgctggtgtt gagcagctta tccttttcaa ttttaagcac
    10501 attctgttcg tatccgattt tcttggaaac cggattattg ttgaccctgt agtagaaaac
    10561 aatgaacctg ctatcgttca gctggaactt gtcaggattt acggggacga gcacctcgtt
    10621 gccgatgacc gaaaatttat cgttgccgaa gtagcttccc aagtcttcaa ccacactttc
    10681 gttgggccta taccgttcag ccataaggct tctgcgctta gtgggtacag caacatcctt
    10741 tacaatggca gtaagctgtt gaatggcatc aggggcagga ggctcatgaa gcttaatcat
    10801 gtagcggccc atattctggc caatggagat aacgtaggag tcaaagttat caaaggtaac
    10861 cttatcgcta ggttggagta cgtctcccgc tctgagagat gttcccgttc ggtagttaac
    10921 cacattcccc ttaattcggg ttacaataaa actatcctga gcaattgccg tggtagccat
    10981 catagccaga agaagagcta gaatatttgc tttagatttc atggcgttag gtttttgtta
    11041 ttgctttttt ttgatttcat caattcctgt accaaaatag cgaaatgttg ggatttgcca
    11101 tccctttaac ttgctccgac aggtgatgca aagtattgcc ctattttttg cctgcagcaa
    11161 ccagcacaaa ggagctatca atccattgca tgacaaggtt tctcggtata agggttgtat
    11221 ccctaagggt tatccagaat tttttgttca tgtggtagcc gggttcaaca aaggtatatt
    11281 tttcctgcag ctcaagggca atttcgggat cacacttcaa ggttaggctg ctatcgcccg
    11341 aaaggcttat catggcgaat attctgccac ccacctttag caccaaggtt tcatcgtcga
    11401 acgggaggct ctcggttgcg ccaggttttg aaaggcagta gtcacgaatt tcttcaacat
    11461 tcataccgtt aaagtgttgg gtaaaaggca tcctcgatat gctctatagc tagtgagccg
    11521 ttggcaataa ccaccgacac aatgttgaaa cgcacctcgg tatcaaggtt gtagtggcaa
    11581 acgtaagcat cggcagcctc caccagccgc ttttgctttt tccttcgaac ggcttctttt
    11641 ggttcctccc agtagtcgct tgtccgagtt ttaacctcca caaccaccag catattccca
    11701 tctctggcca caatatcaag ctccttgtgc ccaaaatgcc aatttcggtg taaaatttta
    11761 tacccttttg atacgaggta ctccgctgcc gaatcctcgc ccagtgtacc taaatccttt
    11821 cgtttgtcgg tgttaacaat ttgccccatt tcgggtgaaa cattatttag cgttatgatg
    11881 tataaacatt acactttagg ttgaaaacca taaaggtcaa aactcactta cttcaacagc
    11941 taacataaac ctaatgatac ggttgaaaga acaaaaatgg ggacatttcc gtcgatttat
    12001 tctggttata cccatgttgt tgcttttccc attcaacctg ctatcaaaca tagacaattt
    12061 tgagggaaaa atcaagtttt taagcgaggg ggtttacgac acgctgagca tggttctcag
    12121 cgtaaagaat gatttggtca ggatggacca gtactccggc aaaaatggtc tggaaatatc
    12181 ctacctcttc gacctgagaa gcggtaaggt ttttgctctt tcaggcaggg aaaaagtgta
    12241 taaggaaatc tacctgcacg atcagtcaaa aaaaagaagg gaaacggacg taaccattat
    12301 caagagccaa aactcaatgt tgataaatgg aacaacctgc taccaatgga gggttaagaa
    12361 taggcagagc atgaccgaag taacctactg ggttgcccaa agtggctttg acttcatgga
    12421 gccccttcaa cggcttattg gccagctgca aacgccattc aacctgttta cgctttgccc
    12481 cgacatcaag tattttttcc cgctgatggt ggttgaccga accatattcc gaaaattcag
    12541 gtgcagccta agggttgtag atataaaacg ggtcgtactc cccgataatt tgttcgctat
    12601 tccccgtggg tatcgagaat tgcttggcta gaactttcgt gaaaggtaag cgaggcaccc
    12661 tgagagttta ccacaagctg cactttacgc cccatgatta atggaaggtt gggttcctga
    12721 tgacccgccg gaaaaccgaa gcataccggt atcctgtact ccttaaccgc ctcactaatt
    12781 atctggtatg cgtccaaccc aaatgggata ttgttgtcct tcatttctga gaaatgaccc
    12841 accaccaatc cagctataga cttgagcttt ccagcccgtt taagctggag catcatgcgg
    12901 tcaacatggt agaggtactc atcaacatcc tcgataaaaa ggatgcgctc ctgtgttgaa
    12961 gtgtcggcat cggttccaat aaggctggcc agtattgaaa ggttacctcc tgttagaaaa
    13021 cctgtagccg ttccttttcg atttagaggg tggtgtggaa tgctatatgc cgggtttctc
    13081 cccattaaaa cgtccaccat gctgctagcc gattcgctat catttatgtt ttttggaaag
    13141 ttaacaggca tggtagcatg aagggtctca atgccaaacc agctggttat aatgctatga
    13201 agaacagtaa tatcgctata tcccactatc catttgggtt taagctggag cttacgcagg
    13261 tttatcctgt ccaaaattct taccgttccg tatcctccac gagcgcaaag aattgccttt
    13321 acgttcgaat cattaaccat ctccatgaaa tcggaagcgc gttcgctatc cgaccctgca
    13381 aactgaaaat gtttggcaaa gagggagggt gccgttttca ctataaaacc ccgtgcagtc
    13441 agaaaccgga tggccggatt aacatcttcg tggctgacaa accttgcagg ggcaataatt
    13501 ccaacagtat cgccaggttt caggtatggg ggagtgatat tctccatgct tcatttaaaa
    13561 attgaccata aatatacatt ttttatttta tctgtttggt tcagcccttt actctttata
    13621 tgcccttttc ttgaaataaa atcaaacctg caggttagcc caactaaatt ttaagtttgg
    13681 caattaatgt tttacctcta ctgtccctcg ggtgaagcaa taaaaaaatc cggcgttaaa
    13741 ctattgccgg aattaagggt aaagcctttg ccacagccca aaagggcaat ccctagtggt
    13801 ttaccaccaa cttttgcgaa aactgcctgc catttgccct gagagctacc aggtaaatgc
    13861 cgttgggctg attgcgggtc aaccaggtta tggtttggtt gccgccacca agttcacctt
    13921 gcgccagggt ggcaataatt tttccgtttc catcgcaaac tattaggttt gccacacaag
    13981 gcttttcaag atgaaatgat atgctaacct cgttcctggc gggattgggc ctgattgggt
    14041 aaaggtagta gccctcagca tcttggccga tcatatctac tccggtgagc gatgggcttc
    14101 cggcctcctt ccatgcagtg tacattaact cggcaagcgc atgtgaagcg ttggcaaata
    14161 gcatggtagt aaattcgcca gttttttccc aaagggcatt cttatatgcc gttgagctgt
    14221 agttggacga aatacctgta gcgtagttgt cggcagccag cacggaatca acatacttat
    14281 aatttgcgta aaggtagttc aggatgtact gcgtaacgtt atcaatctga tgaatttcag
    14341 ccccttcgta ctgtgaaagc tgctgttccc tggcattcac catggtgctt tcgtaacgcg
    14401 agtgaatgcc atcgttgccg gttagctgcc cgttataatt tttagtaatg tgcattggca
    14461 tatgtccatc tgcaacatag tggccaaggt cggctgcaaa aaacttagcc ctatcccagt
    14521 ttagtctggc catgttggat ttgagcgagt caaaagtggc tttagtggca tagggcaaaa
    14581 tgccccagtc gtaaacgtta tagctcccat gaagagtaat tgcttcgttt agggtttgag
    14641 ggatgtatcc cgcgttaata aactcggggt aattttcaat atcaatgtag tgctttggcg
    14701 cttcgttggg atcctcatcc tttcgtttat cggcatcgga ggcatgttca gccaggtagc
    14761 tagcccatgt ctgaaattgc tccatttcca aattaaacga tagcgaagat ttgctactga
    14821 taatgtaatg ccccgtttgt ccccaactgc atagaattaa cccaatggct accagtgcaa
    14881 taagtctagt aaatttattc attttttact cagaattaac aacgcaagtt taaacatttt
    14941 ttaagactgg ttgtttgatg gatttcattt taataggttt aacattcatg gatggacgtt
    15001 ttaactaaaa tatcatctat atttagaaaa attatacact cgccgtctta tagtaacacc
    15061 taatggttcg catcttaaat taacagtagt ttaccgtaaa acactggaaa gtgttgcatt
    15121 agagggcaaa actttttaat attgccaatc ttttaatgaa gcatcgagcc agatttttta
    15181 aaagctggat gatgcacctt aaactctacc tgttattgag ttaatattct tacttatgat
    15241 tacatccgta ctatcgcccg aatttgctca tctacaacca ttttttcata gtatttgctc
    15301 taaatttgat actgaaggag aggttatctt tagcaaacgt aaccagatta aagttttcaa
    15361 catagacggt ctaaaaatct cagtaaaatg ctttaaagtg cctaatctgt ttaacagatt
    15421 ggtgtatagt ttttttcgag cttcaaaggc cgagcgctcc tataggtatg gtttactcgt
    15481 agcaagtttg ggcattaata caccgtcacc tataggctac atcgaggaaa aacaattcgg
    15541 aattattcga agaagctact acgtgtgcgc ctatcagccc taccgggcaa ccataacagg
    15601 catacttgga tcaaatgata ctgacaggga aacgattatc agggaattta cacggttcac
    15661 ctttaccaaa cttcaccaac agggtgttct ccataaggat tactccggag gtaatattct
    15721 ggttgaagac ttgggtgagg acagctaccg cttttcgata atcgacctga accgcatgaa
    15781 ctttaaaaag aaggtatcca ccaaacaggg gctaaaaaac cttagacggc tcactctcga
    15841 cattaccgtg aacgatattg ttacaattac cagcgagtat gcagctttgg ccggaaacag
    15901 caaccccaag caaacgctct tctggttttt gaaataccat ttagactttg tccggttcaa
    15961 gaacttcaaa agttggtata aaaaatttaa aaggggatga acagctcagc caaaacaagc
    16021 cgtatagatt ctggttccaa ggccaacatt ttatcaattt ccacgctctt agcttccacc
    16081 tcgtttctat tccctatact agctgaagaa tcatagcatc tcgcctctta cttttccggg
    16141 taatggtatg gctactcagt gccttcaata ccaagttcac cgctttaaac cattcgtttt
    16201 tttaccagcc tcaaagccaa ccgaccaggg ctctccttca gtgtaggttt acaattttaa
    16261 ccagctcatt cgattgctaa taaatttttc ttagtcccta cttttgagtt ctgagcactt
    16321 gacttttaaa atttaaaagc ttgtccagtg gttttagccc cgaaggggcg gaactgccac
    16381 gcggcacaac tcgccccgcc tacggcgtgg ctcaaacagt gccgcttggg tattcccgcg
    16441 ctacgttccg cccccga
//
LOCUS       DAOPHX010000047        16196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_4081_length_16196_cov_5.942940, whole genome shotgun sequence.
ACCESSION   DAOPHX010000047 DAOPHX010000000
VERSION     DAOPHX010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 16196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16196
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4081_length_16196_cov_5.942940"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PK502_05080"
     CDS             <1..338
                     /locus_tag="PK502_05080"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HPH00224.1"
                     /translation="MQWMDEVAYITATRFTRKKVVTVSVCKVNFVAPVQPNMIVEIRG
                     TVQKVGLVKMEVLVEIYQEDMYSDYRNKAVEATFIFAAVDNDNKPIRLMDSALKLKIK
                     CDSANERYL"
     gene            complement(361..1428)
                     /locus_tag="PK502_05085"
     CDS             complement(361..1428)
                     /locus_tag="PK502_05085"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPH00225.1"
                     /translation="MTDKLHPFFNGEVDEETDEQAELYEHFHFDVDRGQAQIRIDRFL
                     TDKIKNVSRNRIQSAADAGNIVVNGKPVKPSYKVKPLDSISIVMAYPPRDTDIKPENI
                     PLSILYEDDSLLVVDKPAGMVVHPAHGNFSGTLVNALLYHLRDVPLFSSGEVRPGLVH
                     RIDKNTSGILVVAKDEVSMNRLARQFFERTTSRKYIALVWGDMEADEGTIKGNIGRSV
                     RDRKKMQVFTDEEQGKHAVTHWRVVERFGYVTLVECRLETGRTHQIRVHFEYIHHPIF
                     NDEVYGGNKILRGTLHSHYKQFVENCFEICPRHALHAKTLGFTHPLSGMEMNFESPLP
                     NDISALIEKWKKYSTGKYSEK"
     gene            complement(1425..3377)
                     /locus_tag="PK502_05090"
     CDS             complement(1425..3377)
                     /locus_tag="PK502_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPH00226.1"
                     /translation="MKGIRYSSFLAIMTLHVMSVEAQDTLVNLSSYPISNLSSKIQAS
                     SAISKSKSSVQLPFFDDFSRRGVFPTDELWEVTGVTANQLYAVNPPTVGVATFDAVDF
                     TGMLYSYLTTLSTAADTITSLPINLNYLPSDSIYLSFYIQPKGLGYSPSRGDSLVVEF
                     YSPMEGAWVRAWSASVNFADSTLAFRNHLERSSTTTHSNALDRNFFRVMMGITQPEFL
                     SDGFRFRFMNYASISPSQEVQGLKSNCDHWHLDMVYLDKYRNLNDTVINDVAFFKPVK
                     NFLRTYSSVPWKHFTRAFSEEFPSPLEFSVGYRNLGDEVWNVTRRYEIVDESGAQPDY
                     LFMGGAENIHEFQDVSYTRFFSYNFQSAWSDSAKFLLKAYLITENKSPLSLPYYLNFN
                     DTISYNLNFYNYYALDDGTAESGYGLYGEGAENGMVAVRFQNYQADSLSGVMIYFNRT
                     YNNANDIGFKITLWDERNGMPGNIIYQKSGVRPLFTDSLDRFTVYALDKVELKEGSFY
                     IGWQQESSGMLNVGFDMNSNNSSRTLYNLNGEWVETRYNGSVMIRPIFGKLYQIPTNV
                     DIQQSSLGFSIFPNPAQHHIRLSLPDGFTPKAIQLINTAGHVVASRPYETNELFYLEG
                     IAPGFYMVRVSNDMGRCLTGKVIVVK"
     gene            complement(3394..4263)
                     /locus_tag="PK502_05095"
     CDS             complement(3394..4263)
                     /locus_tag="PK502_05095"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPH00227.1"
                     /translation="MENKMTRFFKRISANPYISTILLGIGLFVVLLIVLGISLSIYTR
                     HGQTFAIPDFRGESVDKLPELVRKAGLRMEVTDSVYIFNRAPGSIIDQNPKPGTHVKK
                     NRRVFVTINAKNPIKVEVPDVKGYTLRQAKAILEQQGFEVGKLSFTPDMGVNNVLDQL
                     YKGKPAIPGMLIPKGSKIDLLLGMGMHGERTGLPLLIGVKFEEAKNRIIEASLNVGRI
                     KFDETIETYRDSLEARVYSQYPAYSEAISLSFGTRVDIWLTLNESRIPAIKSFNADSI
                     PAVGSYNGEDIIE"
     gene            complement(4420..5544)
                     /locus_tag="PK502_05100"
     CDS             complement(4420..5544)
                     /locus_tag="PK502_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HPH00228.1"
                     /translation="MQAMLVEREKPLRLTESLGVIVEKVAANQRITVEDAVMLYEDAD
                     LALLSQLANMVCGRINGSNIYFNKNFHIEPTNLCVYSCKFCSYHKAAGHPQSWVLSLE
                     EIRELASRYKGSDVTEAHIVGGVHPKWNIDNYGEIISVVKQVLPEVHVKAFSAIELNY
                     VFRKSGLSPREGFKRLKAHGLNSIPGGGAEIFDSGVRAKICPDKASAEEWLSIHEAAH
                     LEGITSNATILYGHIETYRHRAEHMEAIRNLQDKTHGFSCFIPLKFRSANNPLSYLGE
                     VSLLEDLKNYAVTRIFFDNIDHIKAYWPMLGIENTRLAIAFGADDIDGTIDDTTKIYS
                     MAGAEEQKPAMTVEKLTQLIRESGKVPVERNSLYHAIKYY"
     gene            complement(5675..5830)
                     /gene="rpmH"
                     /locus_tag="PK502_05105"
     CDS             complement(5675..5830)
                     /gene="rpmH"
                     /locus_tag="PK502_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPH00229.1"
                     /translation="MKRTFQPSNRKRRNKHGFRERMSTHNGRRVLSARRAKGRKKLTV
                     SDEIYHK"
     gene            complement(6006..6635)
                     /gene="rsmG"
                     /locus_tag="PK502_05110"
     CDS             complement(6006..6635)
                     /gene="rsmG"
                     /locus_tag="PK502_05110"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPH00230.1"
                     /translation="MVNSSIVTQYFSGLNKDQLEKFERLDDLYGYWNAQINVISRKDI
                     DNLMVHHVLHSLSIARVINFARGTKIMDVGTGGGFPGIPLAIMFPQCQFTLVESVAKK
                     AKVCGEVARSLGLDNVEVLNMRAEALSASFDFVVSRAVATIPQLMEWVEKNIHSGGMN
                     QLPNGLICLKGGSFSDELKPYRKFAQTWRVSNFFDDEYFEEKYIIYIQV"
     gene            complement(6787..7401)
                     /locus_tag="PK502_05115"
     CDS             complement(6787..7401)
                     /locus_tag="PK502_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPH00231.1"
                     /translation="MEIAPENLSEKARYDYELVNRALEGDQKSYAELMERYRDAIYFM
                     LLKMVNNKSDAEDLTLEAFGKAFKKIHQYTPNYAFSTWLFKIATNNCIDFIRKRKANI
                     VSIDQNIDDQEGQYISPTSMLSSGNPDPEEKLIKEQNIQLIQDIVSKLKPRYRKLVEL
                     RYFKEYSYEEIADELNLPLGTVKAQLFRARELLSNILNNLPPHH"
     gene            complement(7392..8540)
                     /locus_tag="PK502_05120"
     CDS             complement(7392..8540)
                     /locus_tag="PK502_05120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPH00232.1"
                     /translation="MKEILSSIETYVLVVGILFIAALVVQLYYYLVVYARVACSKSYK
                     RLLSPEAPKPPVSVVICARNEEENLRHFLPRILEQDYPEFEVVVVNDCSCDESEVLLD
                     RLRKEYPRLQVRTIKEDEKFSHSKKLALTVGIKAARYDWLLLTDADCIPQSDKWIATM
                     ASNFDANAGVVLGYGGYLHQKGWLNKFIRFDTLYIAMNYLGFALMGKPYMGVGRNLAY
                     RKDLFFNNKGFASFVQLDSGDDDLFVSQVATRQNTRVDFRKDAHTRSVPRSSHHLWLQ
                     QKIRHTSTSRYYKPGVKIRLTLEPLSRFLLLASAICLVAFRFFPYWIVALVLFKLSVQ
                     LIIIKSVMVRLGERKILLVSLLWDIYSLYFYPKLLLINRFTRRKTTWR"
     gene            9072..10202
                     /gene="tgt"
                     /locus_tag="PK502_05125"
     CDS             9072..10202
                     /gene="tgt"
                     /locus_tag="PK502_05125"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPH00233.1"
                     /translation="MDFRLIGTDSQTSARAGEIATDHGVIETPIFMPVGTVGSVKGVL
                     HRELENDIDARIILGNTYHLYLRPGLEVLANAGGLHRFSSWSRPILTDSGGYQVFSLA
                     SNRKLNAEGASFRSHIDGSSHFFTPERVMDIQRVIGADIVMAFDECPPGDAEYSYACK
                     SLDLTEQWLKRCVNQFATTEPHYGYSQSLFPIVQGCVYPDLRRRAAEYAVSFNCDGYA
                     IGGLSVGEPAEEMYRMIEVVNYILPKNSPRYLMGVGTPENILEAIALGVDMFDCVLPT
                     RNGRNGMLFTSQGIINLRNEKWKNDFSPIDPLGLTFVDSRYSKAYLRHLFVAKEMLGP
                     QIASIHNLGFYLWLVREARIHIVRGDFLEWKKSMVSRLSQRL"
     gene            10365..11441
                     /locus_tag="PK502_05130"
     CDS             10365..11441
                     /locus_tag="PK502_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPH00234.1"
                     /translation="MKILDWYIIRKFIGTYIYAIILIVGIAVIFDFSEKIDDFMEKGA
                     PFNEIIFRYYLNFIPYFANLFSSLFVFIAVIFFTSNMAYNTEIIAILSSGVSFLRMMV
                     PYFISATIIAIFSLVLTNFIIPPANAVRIDFENKYVKKAYVSSERNIHRQVRPGIFVY
                     MRSFNSYSNIAYNLSIEHFDNNRLKSKLIADYAQWDSTKQTWTIKNYYIRDYLERGEK
                     ITRGQSIDSTLYLTPADFKRRSNVIETMNLFQLNDFIDEQRLQGADNIEALLIEKHSR
                     FANPFSAFILTLIGVSLSSRKVRGGIGLHIGFGLALSFSYILFMRFSTIFAVSGHLSP
                     FIAVWIPNIIFAAIALGLYRLAPK"
     gene            11536..14004
                     /locus_tag="PK502_05135"
     CDS             11536..14004
                     /locus_tag="PK502_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HPH00235.1"
                     /translation="MQPYTTGQIAQILNAKLEGDPNLDINTIATDSRTPSKSKGTLFV
                     AIVGKHHNGHKFISELYNFQHIRAFMVSDTLGLAEKFPQATFIVVKNTLHALQKLATY
                     HRAQFKYPVVGITGSNGKTIVKEWLNQLLSPDYSIVRSPKSYNSQIGVPLSVLQMDSN
                     FNLGIFEAGISLPGEMLRLQPIINPQVGIFTNIGLPHQENFEDLDEKITEKLKLFKSS
                     ELLIYCKDHELVDRIIYNDDTLSGVKLLSWGVHPQSTLHVESTEEHGNGRVIRVKYGE
                     ETFSFEIPFTDQASYENAMHCLSLLFYLNIDRTTIADRMAKLSPVAMRLELKKGVNGC
                     TIINDSYNSDLGSLSIALDFLLQQKQHDNRVLILSDILQSGYAPELLYSMVAQLVEEK
                     EISHIIGIGKNISRYAHLFDISKEFYSNTLEFTQSITRRQFSNSAILIKGSRNFHFER
                     IVAILEEKVHRTVMEINLNSMVHNLNHYRNQLNPGVKLMVLVKAFSYGSGSYEIANLL
                     QFNRVDYLGVAFADEGVSLREAGIALPIIVLNPSYGAYELMIENPLEPEIYNFEGLAE
                     FANALDRMGVNEYNIHLKIDTGMHRLGFTGEQLPELTSTIQSLKSIRIASIFSHLAAS
                     EDSGQDEFTQSQIALFSTISDEIISAIGYKPLRHILNSAGIERFPHAQFDMVRLGIGL
                     HGISPTQQDKLMPVSTLKSKVIQVKHLQPGETVGYNRMGKINKPTTVAAIPIGYADGL
                     NRKLGNGIGKMMINGVLVPTIGNISMDTCTLDATGVDVKVGDDVVIFGSNPTIMEVSQ
                     ALNTIPYEVLTSVSQRVKRIYFQE"
     gene            complement(14013..14723)
                     /locus_tag="PK502_05140"
     CDS             complement(14013..14723)
                     /locus_tag="PK502_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPH00236.1"
                     /translation="MSKKLQHQVDEDSVFEGVCGLFPNLKPEEREKMRNNFSCMFVPK
                     GKIIYREGDTPPGLVCLSKGIVKLYKEGVGGRDQIVRMAKPVGFMGYRALFADENYTA
                     TAQALEDSVVSIIERNALMEILRNNAELTLHILKSVASELGFSTNRTVTLTQKHVRGR
                     VAESLIFLRDTYGFEDDGVTLKAYLSREDMANLSNMTTSNAIRTLSSFAEEGIIELDG
                     RRIKILDQYKLERVSNLG"
     gene            complement(14934..>16196)
                     /locus_tag="PK502_05145"
     CDS             complement(14934..>16196)
                     /locus_tag="PK502_05145"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPH00237.1"
                     /translation="VEVAGKRLAVTVCEDLWDDQPTGEANFRRHKLYRSSPMEHMAML
                     KPDIIINIAASPFSYNIEQQRLQVLQGNIVRYGLPVIYVNQVGGNAELLFDGGSMVLD
                     ANGAVACRLTSFSEDFRVVSSDDFKHSCRSSECSVENQENRIARIHDALVMGISDYFR
                     KMGFSHATLGLSGGIDSAVVLALAHSALGSDAIRVLLLPSQYSSKHSVDDAVTLAARM
                     GIRYDTISIQPIFDSFKSQLADLFQGLPEGVAEENIQARVRGTLLMAISNKFGHLLLN
                     TTNKSEAAVGYGTLYGDMNGAIAVLGDVYKTDVYNLASYINRNQEVIPPSIISKPPSA
                     ELRPNQKDTDSLPPYDVLDRILFAYIEQNHSVKDIIGMGFDSALVHRVIRMVCTSEYK
                     RFQAPPILRVSSKAFGYGRRMPLVSSWT"
BASE COUNT         4430 a         3806 c         3586 g         4374 t
ORIGIN      
        1 ccatgcagtg gatggatgaa gtggcctata ttactgcaac ccgatttact cggaaaaaag
       61 tagtaacggt atcggtgtgt aaagtcaatt ttgttgcccc ggtgcaacct aacatgattg
      121 tagaaattag gggaaccgtt caaaaagttg gacttgtaaa aatggaagtt ttggttgaga
      181 tttatcagga agatatgtat tccgattata gaaacaaggc agtagaagcc acattcattt
      241 ttgctgcagt tgataacgat aacaaaccaa ttcggttaat ggacagtgcg ttaaagctaa
      301 aaattaagtg cgattcggct aacgagagat acctttaatg tattaagcgt gctaatcttc
      361 ttatttctct gaatattttc ctgtagagta ttttttccac ttctcaataa gtgcacttat
      421 atcgttagga agtggcgatt caaagttcat ctccataccc gatagggggt gtgtaaatcc
      481 cagtgttttt gcgtgcagtg cgtggcgtgg gcatatctca aaacagtttt ccacaaattg
      541 tttgtagtgg ctatggagcg tgcccctaag aatcttattt cctccataca cctcatcgtt
      601 aaatatggga tgatgtatat attcaaagtg cacccttatc tggtgtgtgc gaccagtttc
      661 gagacggcac tccacaaggg taacatatcc aaatcgctcc accacccgcc agtgggtaac
      721 agcatgtttt ccttgctctt catctgtaaa aacctgcatt ttcttcctat cccgcacact
      781 acgaccaata tttcctttaa ttgttccctc atccgcttcc atatcccccc ataccagggc
      841 aatgtatttt cgggaagttg ttcgttcaaa gaattgtcgc gccaggcggt tcattgacac
      901 ctcgtctttg gcaaccacaa gtattcccga agtattcttg tctatccgat gaaccaaccc
      961 tggacgtacc tcgccgctgc tgaaaagtgg aacatcccgc aaatggtata gtagggcgtt
     1021 gaccagcgtt cctgaaaagt tgccatgtgc gggatggact accataccag caggtttatc
     1081 aaccaccagt aaggaatcgt cctcgtacag aatgctaagt ggaatgtttt ccggcttgat
     1141 gtccgtatcc ctgggggggt aagccatgac gattgaaatt gagtcaagag gttttacctt
     1201 gtaggatggc ttaacaggtt taccatttac cacaatatta ccagcatcgg cagcagattg
     1261 aatgcggttg cggctgacgt ttttaatttt atcggtcagg aacctatcta ttcggatttg
     1321 ggcttgcccc ctatcaacat caaagtgaaa atgctcgtag agttcggctt gctcgtcggt
     1381 ctcctcgtca acctcaccgt taaagaatgg atggagctta tcagtcattt taccacaata
     1441 actttaccgg tcaggcacct acccatatca tttgaaaccc tgaccatgta aaatcctgga
     1501 gcaataccct cgaggtaaaa gagttcgttg gtctcgtagg gtcggcttgc cacaacatga
     1561 cctgcagtgt tgatcagctg aatagctttt ggggtaaagc catcgggtaa cgataggcgt
     1621 atgtgatgtt gtgcaggatt cgggaaaatg gaaaacccaa gtgaagattg ttgaatgtca
     1681 acattagtag gtatttgata gagttttcca aaaattggtc taatcattac cgaaccgtta
     1741 tacctggttt caacccactc cccgttaaga ttgtagagtg ttctactgct gttgttgctg
     1801 ttcatgtcga atccgacgtt gagcatacca ctcgactcct gctgccatcc gatatagaaa
     1861 cttccctctt tcagctccac cttatcgaga gcgtaaacgg taaatctgtc aaggctatcg
     1921 gtgaatagcg gtctaacgcc cgatttctgg tagatgatgt tgcctggcat cccgttccgt
     1981 tcatcccaga gtgtaatctt gaacccgatg tcgttagcat tgttgtaggt ccggttgaag
     2041 tagatcatta ccccgctcag gctatcggcc tggtagtttt gaaacctaac ggctaccatt
     2101 ccattttcag ccccttcgcc atatagtccg taccccgatt cggcagtacc gtcatccaag
     2161 gcatagtagt tgtaaaaatt aaggttgtag cttatggtat cgttaaagtt caggtagtag
     2221 ggtaaagaga gcggagactt attctctgtt attaagtatg ccttcaagag gaatttagcc
     2281 gaatcgctcc atgccgattg gaagttgtat gaaaaaaaac gtgtatagct gacatcctga
     2341 aactcatgga tgttttcggc tccacccatg aaaaggtagt cgggctgggc acctgactcg
     2401 tcaacaatct catagcgcct ggtaacattc cagacctcat cgcccaaatt tcggtagcca
     2461 acactaaact cgagaggact aggaaactct tcgctaaatg cacgagtaaa gtgtttccag
     2521 ggaacggatg agtaggttcg taggaaattt tttacaggtt taaaaaatgc gacgtcattt
     2581 ataactgtat cgttcaggtt tctatatttg tccaggtaca ccatgtccag gtgccagtga
     2641 tcgcagttgc tcttcaaccc ttgaacctcc tggcttggtg aaatggatgc atagttcata
     2701 aacctaaacc tgaacccatc ggacaggaat tcgggttggg ttattcccat catcacccta
     2761 aaaaaattgc gatcgagggc attggagtga gtcgtagttg atgatctttc aagatggttt
     2821 ctaaaagcca gggtgctgtc ggcaaaattt accgaagctg accatgctct aacccatgca
     2881 ccctccatgg ggctatagaa ttctacaacc aacgagtcgc ctctggaagg gctatagccc
     2941 aaccccttgg gctgaatgta aaacgaaagg tagatgctgt cggaggggag gtagttcagg
     3001 ttaatgggca acgaggtgat ggtgtcggca gctgtggaca gggtggttag atatgagtaa
     3061 agcatacccg tgaaatcaac tgcatcgaag gtggctacgc ccacagttgg gggattaaca
     3121 gcataaagct ggttggcagt aaccccggtt acctcccaca gctcgtcagt tgggaaaacg
     3181 cctcttcttg agaagtcgtc gaaaaaagga agttgaacag aagatttcga tttgctgatt
     3241 gctgagcttg cttgaatttt agacgaaaga ttgcttatag gatatgaaga aagatttacc
     3301 aatgtatcct gagcttccac cgacattacg tggagagtca taattgccaa aaaggaggaa
     3361 tacctgatac ctttcatgaa gattttgaag tagctactca attatgtcct ccccattata
     3421 gctgccaacg gcaggaatag agtcggcatt aaagctctta atagctggga tacgcgactc
     3481 gtttagggtg agccatatat ccacccgtgt tccaaaacta agagaaattg cctcagagta
     3541 tgcaggatat tggctgtaaa ccctagcctc caacgaatcc ctatatgttt cgatggtttc
     3601 atcaaactta atgcgaccaa cgtttagcga ggcctcaata atcctattct tggcctcctc
     3661 aaacttaact ccaataagca acggaaggcc tgttcgctcg ccgtgcattc ccataccaag
     3721 cagcaggtca attttagagc ctttagggat gagcattccg gggatggctg gtttgccttt
     3781 ataaagctgg tcgagcacat tgttcactcc catgtcgggt gtaaaggaca gctttccaac
     3841 ctcgaatccc tgctgttcta gtatggcctt agcttggcgc agggtgtacc ctttcacatc
     3901 gggtacttca actttaatgg gatttttagc attaatggta acgaataccc gcctattctt
     3961 cttcacatga gttccgggct taggattctg atcgataatg ctcccggggg cgcggttgaa
     4021 gatgtaaacc gaatcggtaa cctccattct tagccccgct tttctgacaa gttcgggcaa
     4081 cttgtcaacc gattcacctc tgaaatcagg aatggcaaag gtttgtccat gccgggtgta
     4141 tatgcttagc gaaatcccta acactatcag caatacaaca aaaagcccaa tacccagcag
     4201 aatagttgaa atgtagggat tggccgaaat tctcttaaaa aaacgtgtca tcttattctc
     4261 cataaccgta ggtgtggaag tggcaaattt aacactattg cagcaacaaa aaagcgtttg
     4321 ctaccgccga ttttctaata accagatcct agcaaaaagt agcaaccatg agcatgaaaa
     4381 aatcattgta taaaaatttg tcaatttaga taactgcacc tagtagtatt ttattgcatg
     4441 gtaaagcgaa ttgcgttcaa cgggaacttt gccggattct ctgattagct gggtaagttt
     4501 ttcaaccgtc atggcaggtt tttgctcctc ggcaccagcc atggagtata ttttggtggt
     4561 atcgtcaata gtgccatcaa tgtcatcggc tccaaaagca atggccaaac gggtattctc
     4621 aatgcccagc atgggccagt aggctttaat atggtcaatg ttatcgaaga agatacgcgt
     4681 aacagcgtag ttcttaagat cctccagcag gctaacttcg cccaggtagc taagggggtt
     4741 attggcagaa cgaaatttca ggggtataaa gcagctaaaa ccatgggttt tatcctgtag
     4801 atttcggatt gcctccatgt gctcagctcg gtgcctgtag gtttcaatat gtccgtatag
     4861 aatagtagcg ttggatgtga taccctccag atgtgccgct tcgtggatgg agagccactc
     4921 ctccgccgaa gctttgtcgg ggcaaatttt agcacgaacc ccagagtcga aaatttcagc
     4981 gccaccgccg ggtattgagt taagcccgtg agctttcaac cttttaaatc cctcgcgggg
     5041 agaaagcccg cttttgcgga aaacatagtt cagctcaatt gccgagaatg ccttgacgtg
     5101 gacttcaggg agaacctgct ttaccacaga aataatttca ccatagttgt cgatgttcca
     5161 ctttgggtgt actcctccta ctatgtgagc ctcagttaca tcgcttcctt tataccggct
     5221 tgctagctcc ctaatttcct caaggctcaa cacccagctc tgcggatgtc ccgctgcttt
     5281 atggtaggaa caaaacttac agctgtaaac acaaaggtta gtcggctcaa tatgaaaatt
     5341 tttattaaaa tatatgttac tgccatttat tctgccgcat accatgttgg caagctgcga
     5401 gagcagcgcc aaatctgcat cctcgtagag cataactgca tcctcaaccg taattcgttg
     5461 gttggcagca accttctcca ctataacccc aagtgattcg gttaaccgta acggtttttc
     5521 ccgttcaacc agcatagctt gcattaaagt ccatattaat tttcacccaa agatagcttt
     5581 tcggtaaaca acgaatgttg ccttaaagtt gcacaaaaaa taaaaggcag gtttcatccc
     5641 gccttcccat taggaaatat tcttaccaac aaatctactt atggtaaatt tcatcggaaa
     5701 ccgtcaactt ttttctgcct ttagccctac gggctgaaag taccctgcgg ccgttatgtg
     5761 ttgacatgcg ctcacgaaaa ccatgcttat ttcttctttt cctgtttgaa ggctggaatg
     5821 ttctcttcat ctccgttatc tttttgtatt attacttttg ccttatagac ctgatggact
     5881 tgtagcgtat tcctttctca aatttgagtg tgcaaagtta aaaataaatt tttctgaaaa
     5941 aaatagtgta gggtaattat taccactatt aaatcttaaa tttagcatat tcctgtttat
     6001 caacactata cctgaatgta aattatgtac ttttcctcga agtactcatc atcaaaaaaa
     6061 ttactcaccc tccaggtttg cgcaaactta cggtagggtt ttagctcatc ggaaaaacta
     6121 ccccctttta agcagatgag cccgttaggc agctggttca tcccgcccga atggatattc
     6181 ttttcaaccc attccatcag ctgcggaatg gtggcaaccg cacggcttac aacaaaatcg
     6241 aaacttgctg atagtgcttc ggctcgcatg ttcaacacct caacattgtc gaggccaagc
     6301 gatcgggcaa cttcaccaca taccttagcc tttttagcca cggattctac cagggtaaac
     6361 tggcattgag ggaacatgat agccagagga atacccggaa atccgccccc tgttcccaca
     6421 tccattattt ttgttcccct tgcaaagttt ataaccctgg caattgacag ggaatgcaga
     6481 acgtggtgaa ccatcaggtt gtcaatatcc ttacgtgaaa ttacgttgat ttgtgcattc
     6541 cagtatccat agaggtcatc gagcctttca aatttctcca gctggtcctt attcagacct
     6601 gaaaaatact gggttactat actgctgttc accatttctc caacttataa cttaatggtt
     6661 atcaataatg taaaataatt accaagcaaa aatttaggag tttacctatt gccataatgt
     6721 ttggccgcta ctagataaca tgcggttacc aaccctttct accacgcact gccagccatt
     6781 tttggtttaa tggtggggtg gtaaattatt caggatgttg gaaagcagct ctcgagccct
     6841 gaaaagttga gccttaaccg ttcccaatgg aaggttaagc tcgtcggcaa tctcctcgta
     6901 cgagtactcc ttgaagtacc tcaactccac aagttttctg tagcggggct ttagcttgga
     6961 aacaatgtcc tgaataagct ggatgttctg ttcctttatc aacttttcct cggggtcggg
     7021 gtttccggaa gaaagcattg aggtaggtga aatgtactgg ccttcctggt catctatatt
     7081 ctggtcaatg gaaactatgt tggctttacg tttacgaatg aaatcgatgc agttattggt
     7141 agcaattttg aaaagccagg tgctgaaggc gtagtttggg gtgtactggt gaattttctt
     7201 aaatgcctta ccgaaagctt caagggtcag gtcctccgca tcgctcttgt tgttcaccat
     7261 tttcagcagc ataaagtaaa tggcatcgcg ataacgctcc ataagctcgg cataggactt
     7321 ctgatctccc tcgagtgccc tgttaaccag ctcatagtcg tagcgcgctt tttccgatag
     7381 attctcggga gctatctcca tgttgttttc cgtctggtaa aacgattaat aagtagaagt
     7441 tttgggtaaa aatacagtga atatatatcc cacaaaagcg agaccagcaa aatttttcgt
     7501 tcacccaatc ttaccataac agattttatg ataattagtt gaacagatag tttaaaaagg
     7561 actagggcaa caatccagta ggggaaaaaa cgaaaggcaa ccaggcaaat tgctgatgcc
     7621 agcagcagga aacgggacaa gggttcaagt gttaacctga ttttaactcc tggtttgtag
     7681 tagcgcgagg ttgaggtatg cctaatcttt tgctgcaacc aaaggtggtg tgagctgcgt
     7741 ggaaccgaac gcgtgtgggc gtctttgcgg aaatccacac gcgtgttctg acgggttgcc
     7801 acctggctaa cgaaaagatc atcatcgccg gaatcgagct gaacaaatga ggcaaatccc
     7861 ttattattaa aaaacaaatc ttttcggtag gcaaggttgc gtcctacacc catgtagggt
     7921 ttacccatta gggcaaagcc caggtagttc atggctatgt agagtgtgtc gaagcgaata
     7981 aacttgttaa gccagccttt ttgatgaaga tagccaccat agcccagcac cacacctgcg
     8041 tttgcgtcaa aatttgaagc catggttgca atccacttat cgctttgcgg gatgcaatcg
     8101 gcatcggtaa gcagcagcca atcgtaccgg gctgccttaa ttccaactgt tagcgcaagt
     8161 tttttagaat ggctgaactt ttcatcctcc ttgattgttc gcacctgcaa acggggatat
     8221 tccttgcgga gcctatcaag cagcacttca gattcgtcgc aagagcagtc gttaaccaca
     8281 accacctcaa attcagggta atcctgttcg agaatacgcg gaagaaaatg gcgtaaattt
     8341 tcctcctcat ttcgggcaca aatcacaacc gaaacaggcg gtttgggagc ttcggggctc
     8401 aacaatctct tgtagctttt actacaggca actcgggcgt aaactaccag gtagtagtag
     8461 agctgaacaa ccagggcggc aataaacaaa atgcccacca ccagcacata ggtctctatg
     8521 ctgcttaaaa tttctttcac cgctattcat tgtttaaaaa atccagcaat aatatcaaac
     8581 atttttgggt ttacccaaca ataaaggagt aatttgctaa actacctgtt tgaaccagac
     8641 atttaggcga ttacaaacat gaggtaacag gtgaaatagc gccggcaggt tgttgaggcg
     8701 gtagcaaaag tagctagggt tttgggcgaa atctatagcc cgaaccaggc caaacaactc
     8761 aaaagcctcg ttgatagcat atcggtgagt tcggatgatt agcatgacaa gacacctgct
     8821 tgtgccttgt catgctgtaa aaataaaaga aactctacta tttatgctta cctgaaagtt
     8881 gcaagcagtc tcacatggac actcaatttg acgtgtaaaa ttttttggta tttttaacaa
     8941 aagctacagc ctgtaaaact accggtaaca aaacaagcta cccatttcta tatacaaatt
     9001 attgttcgta gggcaaaacc ataccgcccc acatagctaa ttttgctatt tttgcggcga
     9061 aaatatacag gatggacttt cgactaattg gcaccgacag ccagacctct gcacgggcag
     9121 gtgaaatagc aaccgatcat ggagtgattg agactccgat tttcatgcct gttggaaccg
     9181 ttgggagtgt taagggggtg ctccaccgcg agctggagaa cgatattgac gcccggataa
     9241 ttctgggcaa cacctatcat ctctaccttc gtcccgggct ggaggttttg gccaatgccg
     9301 gtgggcttca ccggtttagc tcatggagcc gccccatact taccgacagc gggggctacc
     9361 aggttttttc gctggcctca aaccggaagc taaatgcgga aggcgcttcg ttccgctcgc
     9421 acattgacgg gtcatctcat tttttcaccc ccgaaagggt aatggacatt cagcgggtaa
     9481 ttggcgccga tatcgtgatg gcatttgacg agtgcccccc cggggatgcc gagtacagct
     9541 atgcctgcaa gtcgctcgat ttaaccgaac aatggcttaa aagatgtgta aaccagtttg
     9601 caaccaccga accgcattac gggtatagcc aatccctttt cccgatagta cagggttgcg
     9661 tgtaccccga tttgcgaagg cgagccgctg agtatgccgt ttcgttcaac tgcgatgggt
     9721 atgcaattgg cggcctttcg gtgggcgaac ctgccgagga gatgtaccgg atgattgagg
     9781 tggtgaatta catactccca aagaactccc ctcgctacct catgggcgta ggaacacccg
     9841 agaatattct ggaggctata gccctgggag ttgacatgtt cgactgcgtt cttcccaccc
     9901 ggaatggccg taatggaatg cttttcacct cacagggcat aataaacctg cgaaacgaaa
     9961 aatggaaaaa cgatttttca cccattgacc ctcttggcct aacttttgtt gattcacgct
    10021 actccaaagc ttacctgcgg cacctgtttg tggccaagga aatgctgggc ccacaaattg
    10081 ccagcatcca taacctgggg ttctacctct ggctggttag ggaggccaga atacatattg
    10141 ttcggggaga ttttttggag tggaaaaaaa gtatggttag cagactttca caaagattgt
    10201 agcctgtgcc attcgaaagc tacaaaactc aatcttcagg aaggtgaagc tgctctcaaa
    10261 atgtaaaaaa atgaagtatt ctttagccaa agagccgcta agtattagct ggcatacaat
    10321 caagtaacaa tatttactaa ttttcgccct caaaacttaa tgccgtgaag atactcgact
    10381 ggtacataat ccgcaagttt attggaacat acatatatgc cattatcctg attgtaggca
    10441 tagccgttat attcgacttt tcggaaaaaa ttgacgactt catggaaaag ggtgcaccct
    10501 tcaacgaaat catattccgg tactacctaa actttatccc ctactttgcc aacctgttca
    10561 gctcgctatt cgtttttata gcggtgatat tctttacctc caacatggcc tacaacaccg
    10621 aaataatagc cattttgagc agcggggtta gctttttacg gatgatggtg ccatacttta
    10681 tttccgccac catcattgca attttctccc tggtgctcac caacttcatc attcctccag
    10741 ccaacgctgt aagaattgac tttgagaata agtatgtaaa aaaagcctac gtgagctccg
    10801 agcgcaatat ccataggcag gttcgacctg gaatctttgt ttacatgcgt tcatttaaca
    10861 gctacagcaa cattgcctac aacctttcca ttgagcattt tgataacaac cggctaaaat
    10921 cgaagctgat tgccgattat gcccagtggg actcaaccaa gcaaacctgg acaattaaaa
    10981 actactacat acgcgattac cttgaaaggg gcgaaaagat tactcggggc caatccatcg
    11041 atagcacttt atacctgact cccgctgatt ttaagagacg ttcgaacgtg attgaaacaa
    11101 tgaacctttt tcagctaaac gacttcattg acgagcagcg gctgcagggt gccgataaca
    11161 ttgaggcact tttaatagag aagcatagcc gctttgccaa tcccttttcg gctttcattc
    11221 tcacccttat tggggtttcg ctttcgtcac ggaaagttcg cggcggcata gggctgcata
    11281 taggtttcgg cctagccctt agcttctcct acatcctatt catgcgcttc tccaccattt
    11341 ttgcggtgag cgggcacctc agcccgttta ttgcggtgtg gataccaaat attatctttg
    11401 ctgcaatcgc cttagggctc taccgtttag cgccaaagta aattctgtag cttaatcatc
    11461 ctgtattaca tgctgagcag aatcaacatt ggaaatttcg tgtaaatttg tacggagtat
    11521 ccacctataa atactatgca accttacaca acagggcaaa tagcacaaat tcttaacgcc
    11581 aaactcgaag gcgatcctaa cctggatatc aacacaattg ctaccgacag ccgaacccca
    11641 tccaaaagca agggaacgct ttttgttgcc atcgttggga aacaccacaa cggtcataaa
    11701 ttcatcagcg agctttacaa ctttcagcac ataagggcat tcatggttag cgacaccctc
    11761 gggctggccg aaaaatttcc ccaggctacc tttatcgtcg ttaagaatac cctgcatgca
    11821 cttcaaaaac tagccaccta ccatcgggca cagtttaagt acccagttgt cggaatcact
    11881 ggaagtaacg gcaaaaccat tgttaaggag tggctaaacc agctcctttc gcccgattac
    11941 agcatcgtac gcagccccaa aagctacaac tcccaaattg gagtccccct ttcggtgttg
    12001 caaatggaca gcaacttcaa cctcgggata tttgaagcgg gcatttccct tcctggcgag
    12061 atgctgcgac ttcaacctat cattaatcct caagttggaa ttttcaccaa tattggcctt
    12121 ccacatcagg aaaatttcga ggatttggac gaaaaaatta ccgagaagct taaacttttc
    12181 aaatcatccg agctgcttat ttactgtaag gatcacgaac tggttgatcg tatcatctat
    12241 aacgatgaca cgcttagtgg ggttaagctg ctttcatggg gtgttcatcc ccagtcaacc
    12301 ctgcatgtag agagtacgga ggagcatggc aacggtagag ttattcgggt aaagtatggg
    12361 gaagaaacgt tcagctttga gattcctttt accgaccagg cctcctacga gaatgccatg
    12421 cactgcctaa gcttgttgtt ctaccttaat attgatagaa ccaccatcgc tgaccgtatg
    12481 gccaagctaa gccccgttgc catgaggctg gagttgaaaa aaggggtaaa cggatgcacc
    12541 attatcaacg atagctataa ctccgacctg ggctcgcttt ccatagccct cgatttttta
    12601 ctccagcaaa aacaacatga caaccgcgta cttatccttt cggatatcct gcaaagcggc
    12661 tatgcccccg agctgctgta ctccatggtt gcccagctgg tggaagaaaa ggaaatatcc
    12721 catatcattg ggattggtaa aaatatttca cgctatgctc acctcttcga tataagcaag
    12781 gagttttaca gcaatacgct tgagttcaca caaagcataa cccgaaggca gttcagcaac
    12841 tccgcaatac tcatcaaggg aagccggaac tttcactttg aacggatagt agccatttta
    12901 gaggaaaaag ttcatcgaac ggtgatggag ataaacctga actcgatggt gcataacctg
    12961 aaccactacc gcaaccagct caacccggga gtaaagctga tggtgctggt aaaagctttc
    13021 tcttatggca gtggttcata cgaaattgcc aacctcctgc aattcaacag ggttgactac
    13081 ctgggagtgg catttgccga tgagggtgta tcgcttcgtg aggccggtat tgctctgcct
    13141 atcattgttc tcaatccatc ctacggcgcc tacgagctga tgattgaaaa cccgcttgaa
    13201 ccagaaatat acaactttga aggacttgcc gaatttgcca atgccctgga caggatgggg
    13261 gtgaatgaat acaacattca tctgaagatt gataccggta tgcatcgcct ggggtttacc
    13321 ggcgaacagc ttccggagct gacatccacc attcaatcgt taaaaagtat aaggattgca
    13381 agcatcttct cgcacctggc tgcaagcgag gattccggac aggatgagtt cacccaaagc
    13441 caaatagcgc ttttcagcac catatcggat gaaatcatca gtgcaatagg ctataagcca
    13501 cttcgccaca tcctgaatag tgctggtatc gagcgttttc cccatgccca gttcgacatg
    13561 gttaggctgg gaattgggtt gcacggtatt agccctaccc agcaggacaa gctaatgccc
    13621 gtcagcacgt taaagagcaa ggtgatacag gtaaagcatc tgcaaccggg cgaaacagtt
    13681 gggtataacc gtatgggcaa gataaacaaa cctactacgg ttgctgccat tcctattggc
    13741 tacgccgatg ggttaaaccg taagctgggc aacggaatag gaaagatgat gattaacgga
    13801 gtgctggtgc caaccattgg aaatatcagc atggacacct gcacactcga cgccaccggg
    13861 gttgatgtga aggttggaga cgacgtagtt atttttggta gcaatccgac tatcatggaa
    13921 gtctcccaag cgctaaacac tatcccctac gaggtgctaa cctccgtttc gcaaagggtt
    13981 aaacgcattt actttcagga gtaaccggct agctaaccta aattgctcac tctctcgagc
    14041 ttgtactgat cgagaatttt aattcttcgg ccatccagct caattattcc ctcctcggca
    14101 aaactcgaaa gggttctaat ggcgttagag gtggtcatgt tcgacaggtt ggccatatcc
    14161 tcgcgcgata ggtatgcctt tagcgttacc ccatcatcct cgaagccgta tgtatcgcga
    14221 aggaagatga gcgactcggc cacgcgtccc cgaacatgct tctgcgtaag ggttaccgtt
    14281 cggttagtgg aaaatccgag ctcggaagca accgatttaa gaatgtgcag ggtaagctca
    14341 gcgttgtttc tgagaatttc catcagcgcg tttcgctcta taatgctaac cacagaatct
    14401 tcgagagcct gtgcagtggc ggtgtagttt tcatcggcaa ataaagccct gtagcccata
    14461 aagcctaccg gtttggccat gcgcacaatt tgatcgcgtc cccctacccc ttccttatag
    14521 agcttcacaa taccttttga aaggcaaacc aagccgggcg gtgtatcgcc ttccctgtag
    14581 ataatcttac ctttaggcac aaacatgcag gaaaagttgt tccgcatttt ctcgcgctct
    14641 tcgggtttca gatttgggaa caaaccgcaa acaccctcaa aaaccgaatc ttcatcgact
    14701 tgatgctgca attttttaga cataaccttt ttttcaaaaa tagggctttt tatagttaaa
    14761 aaacatgttt ttaacaaagt ttaataggca gcgctatcga tgctgtataa tatcctttta
    14821 aagtaaaaaa gtttttggcc atggaatatc aagcattcaa taaaaagttg aaaaacaaaa
    14881 acaataatag tgtttagcgt gtaggattaa tggccgcttc tttcaaccgg taattacgtc
    14941 cacgatgaaa ctaatggcat gcggcgtccg tagccaaacg ccttcgacga aacccttaaa
    15001 attggaggcg cctgaaatcg cttgtactcg cttgtgcaaa ccatccgaat aacacggtgc
    15061 accagagcgc tgtcgaatcc catgcctata atgtccttca cgctatggtt ctgctcaatg
    15121 taagcaaaaa gaatccgatc gagcacatcg tatgggggaa gtgaatcggt atccttctgg
    15181 ttggggcgta gctcggcaga tggcggttta gagataatgc taggagggat tacctcctga
    15241 tttctgttaa tatagcttgc caggttgtac acatcggttt tgtaaacatc acccagcacg
    15301 gcaatggctc cgttcatatc gccgtatagc gttccataac caacagcagc ttcactttta
    15361 ttggtagtat ttaaaagtaa atggccaaac ttattcgaaa ttgccatgag cagcgttccc
    15421 ctaacccttg cctgaatgtt ttcctcggct acaccctcag gcaatccctg aaacaggtcg
    15481 gcaagctggc ttttgaacga gtcgaaaatg ggctgaatac tgatggtgtc gtaccgtatt
    15541 cccattctgg cggcaagggt tacggcgtca tccaccgagt gtttcgatga gtactgcgag
    15601 ggtaaaagca gcaccctgat ggcatcggaa cccaaggcgc tatgcgctaa ggccaggaca
    15661 acagctgaat caattccgcc ggaaagccct agcgtggcat ggctaaaacc cattttccgg
    15721 aagtagtcac taatgcccat caccagagca tcgtgtattc gggcaattct attttcctga
    15781 ttttcaacag agcattctga tgacctgcaa gagtgcttaa aatcatcgga gctaaccact
    15841 ctaaaatcct cagaaaaact ggtaaggcgg caagctactg ccccattggc atcgaggacc
    15901 atggaaccgc cgtcaaacag cagctcggcg ttaccaccaa cctggttgac atatataacc
    15961 ggcaacccgt aacgaacgat atttccctgc aaaacctgca accgttgctg ctcgatattg
    16021 tagctgaacg gagaagcagc aatgttaata atgatgtcgg gctttaacat cgccatgtgt
    16081 tccatagggc tgctgcggta tagcttatgc cgcctgaaat ttgcttcacc ggtaggctga
    16141 tcatcccata aatcctcaca cacggtaaca gccaggcgtt ttccggcaac ctcaac
//
LOCUS       DAOPHX010000048        16107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_4117_length_16107_cov_5.887802, whole genome shotgun sequence.
ACCESSION   DAOPHX010000048 DAOPHX010000000
VERSION     DAOPHX010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 16107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16107
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4117_length_16107_cov_5.887802"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..838)
                     /locus_tag="PK502_05150"
     CDS             complement(146..838)
                     /locus_tag="PK502_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00238.1"
                     /translation="MDEKPFFSFDITKIKDQQIKELIKFHKNGFDIGAILNTASELKY
                     SNAIKTILNSQLANPSPEFVKFFVSRVYDGKATEKVMIQFTEIVKKTVEQTFNDTVSD
                     RLMNAINQTKPTEDSTKAKENEEESDENKLITTEDEINSFYIVKSILRTKIDSTRIFF
                     RDSQTYFSVILDDNNRKPVCRLWLNGQSKKYIVLFDKEKKETKHELQSLDDIFNFTNE
                     LLSTVEFYNNEK"
     gene            complement(854..1048)
                     /locus_tag="PK502_05155"
                     /pseudo
     CDS             complement(854..1048)
                     /locus_tag="PK502_05155"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
     gene            complement(1073..1216)
                     /locus_tag="PK502_05160"
     CDS             complement(1073..1216)
                     /locus_tag="PK502_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00239.1"
                     /translation="MEFKDQIKQLGEKVLKLRDQILTEEATKSAFVLPFIQVLGYDVF
                     NPM"
     gene            complement(1417..2454)
                     /locus_tag="PK502_05165"
     CDS             complement(1417..2454)
                     /locus_tag="PK502_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00240.1"
                     /translation="MKKWIITGISGSGRIELLNELEKYANDRGRKTQVHDVGRLIYNE
                     ASKLDIQLTDNRVLDIDRNLLSTLRENALKEIEILKLKNPEVELHLIGIHATFRWKHR
                     IIEGLSFRNLSRFKIEGFINIVNDVKEVFRINSINPKWDNDTLPNLEETQNWMIEEEF
                     VTQVIADFFNKPVYIIARNHNIHNLFDFFYTNKKKIYLSYPITAIKDSNPELLTQIQT
                     EFLEKLEKLFVVFNPLAIKDMSLTYGNKIELPNTIADVTDTAKKIIKARTIERDFRFI
                     DQSDATVVIYPTDKLSPGVLAEIIYSHNNQKPVFVYFSGSKSPFLENYASYMTSDFND
                     LLTKLDDFAND"
     gene            3237..4757
                     /gene="rmuC"
                     /locus_tag="PK502_05170"
     CDS             3237..4757
                     /gene="rmuC"
                     /locus_tag="PK502_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPH00241.1"
                     /translation="MTGIVLVGILVVLAVVGISILLIRKPTVDIKPQLREVENLMVGF
                     GATLDRVEKSIKDEFQRNRAETNEISKTNREELTKSLDTFSSTFEKSVKELNDVLKAK
                     YDELRNQQADFNRQISDANKANREELANSLKSFETKFTENIRELNELLRQKFSDFSQQ
                     QTEVNRQLTENIKDVKQSVDNQLKSIREDNTHQLDEMRKTVDEKLQKTLNDRLSQSFE
                     TVGKQLQAVQVGLGEMKNLAADVGGLKRVLSNVKMRGGIGEIQLSMLLEQILAPDQYE
                     ANVKTKQNSADLVEFAIKLPGKDDSGKNVWLPIDAKFPKDIYEQLQDAYDAGDQNLIL
                     NAQKNLDSTIKKMAKDISDKYLDPPDTTDFGIMFLPFEGIYAEVVRKAALLEDLQRNY
                     KVIVTGPTTLAAILNSLQIGFKTLAIQKRSSEVWQVLGAVKREFENFGGLMEKAQKNI
                     QTGLNQLDDVMGKRTRAIQKKLKSVEALSEAEAKSILPEISESAIVDDEDDDLGEV"
     gene            4851..7739
                     /locus_tag="PK502_05175"
     CDS             4851..7739
                     /locus_tag="PK502_05175"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPH00242.1"
                     /translation="MLKAKILMGSLLIAASLAGISQEVQMDNSRVKQLTLNNGLTVIL
                     DENHAQPKVFGLVMVKAGGKNDPADATGMAHYQEHMLFKGTQTLGTINWKEEKPYIDK
                     IFQLYDSLGKTSDEAKRKQIQQEINEASLKANSFAIPNEFSNLVNEIGGSMLNAGTGP
                     DYTVFYNMFPSSQIERWLDLYAHRFVEPVFRGFQAELEVVYEEKNLYNDQFFTKLLEE
                     FQKQFFKNHPYGQQTLIGSIEDLKNPSLTKMYDFFKTYYVPNNMALVLSGDFNSDEII
                     PVIEEKFGKWKEHELPESKIWTEKPFNGREFYQAKLSPVKMGLLGFRAPSAKDDGFLA
                     AEVATRILNNSYSTGLLDKLTLDGKLLAAQALQMPYLDHGAVIVVMLPKILGQSLEDA
                     ESLVNNELGKLKRGEFDDWMLEAVKLDLYRNHVTTMEKIDGRALYLGEAFAKGQSAQE
                     ALDYPNKIMRLTKDDVVAAANQIFGDSYLAFYSKMGFPKKEKIEKPDFKPLLANTEAK
                     SEYAKHFFNLPSKKPDFNPIVFDRDVETVEIGNGHKLMCVQNPANDIFSLTINFMIGE
                     NTMPLLRLASQGMGLSGAGSYNVTELKSEFARIGASYNITSNDYSVSIELQGVESELD
                     RALELVGMLVGDPRLEQSKIKTIVDGETTARKVERSQPDNVAQALLEYTLYGDSSNYL
                     NRLSSKQLKNLKAEELVSTFKKATEYNTQIYYCGKTGASNVAAEVSKHVPLAANPKHD
                     TNSKDRLAKHYNENTIYLVDKPKARQSKVFVFINGQSFTPADAAVIDAFNDYFGGGFS
                     GLILQEIREYRSLAYSAGGKFKLPRQKGNPADFIGYVGTQSDKTLTAMETFNGLMRQM
                     PEKPERMGLIRSHLELLAQTDRPNFRELASRVDEWRDQGFEADPAFVKLPAYQLLKWQ
                     AIEQFYADRMKGKPAVYMIVGDKKQINLKELSSFGKVIELKEDKLFNK"
     gene            complement(7802..7875)
                     /locus_tag="PK502_05180"
     tRNA            complement(7802..7875)
                     /locus_tag="PK502_05180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7839..7841),aa:Arg,seq:acg)
     gene            complement(8179..8544)
                     /gene="rplS"
                     /locus_tag="PK502_05185"
     CDS             complement(8179..8544)
                     /gene="rplS"
                     /locus_tag="PK502_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPH00243.1"
                     /translation="MDLIKVAEESFASKKEHPSFKSGDTVVVHYKIVEGSKERIQNYR
                     GVVIQRRGVGATETFTVRKISGGVGVERIFPLNSPFIEKIEVAKEGKVRRARIYYLRN
                     LTGKKARIKEKKRKLDIGE"
     gene            complement(8915..10669)
                     /locus_tag="PK502_05190"
     CDS             complement(8915..10669)
                     /locus_tag="PK502_05190"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPH00244.1"
                     /translation="MRKLAVQIFTLVSFAFLADTGWACTNFLVTRGASTDGSTFITYS
                     ADSHYLFGELYFRPAADYPAGTMMKIYEWDTGKYLGEIPQVGHTYSVVGNMNEYQVSI
                     GETTYGGRLELQDSTALIDYGSLMYIALQRSKSAREAIKVMAELVEQYGYYSEGESFS
                     VADKNEVWIFEMIGKGTNLKVDKKTKKSYNADKGAVWVAIRIPDGYISGHANQARITT
                     FPQENLKNSISSRNLDKIFDPGVEVVYSSDVIDFARTKGYYTGTNEDFSFSDTYAPLD
                     FGAARACEIRVWSFFKEVNQSMWDYFDYAKGHNLKHRMPLYIKPERKISVKDMMNFMR
                     DHLEGTELDMRKDPGAGPHAMPYRWRPMEWKYEGKEYVNERATATQQTGFSFVAQARN
                     WLPDPIGGIFWFSVDDVASTVYFPAYCGITAVPESYAEGKGDMLTYCDHCAFWTFNKV
                     SNFAYLRYDLMSQDIKKVQDELEGRFISNTQVVDATALKLYEQNQSEAIRFLTDYSVN
                     TGDYVVTRWEKLFQFLLLKYMDGNVKQVDENGQFIYNEYHQCPARVSNPEYPDWWKKA
                     VIDATGDKLLVPENKGGH"
     gene            complement(10769..11818)
                     /locus_tag="PK502_05195"
     CDS             complement(10769..11818)
                     /locus_tag="PK502_05195"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HPH00245.1"
                     /translation="MSVVGVMSGTSLDGLDICLAHFEKKSDSWAYTIEAANTVPYSAE
                     VKIKLSDIEKLTAWEISKLDYDYGHFIGKEVFSFLKQINRHADVVASHGHTAFHNPGM
                     GYTFQIGKGAAIVAETGIPCISDFRAADICRNGQGAPLVPIGDKLLFTSYDICLNLGG
                     IANISFDNVDNERVACDVAPCNMLLNFLSLQLGMEYDAYGNAGAKGMVNHDLLNRLNE
                     LEFYGLLGPKSLGREWFEQKVVPVFASCNASLQDKLRTAYEHIAIQISKSTAEISPGA
                     MLVTGGGARNRFLVQLITEKVKHRVVVPENMLVDFKEALIFAFLAALFLNGTPSALAS
                     STGATADSISGCLYL"
     gene            complement(11845..12549)
                     /locus_tag="PK502_05200"
     CDS             complement(11845..12549)
                     /locus_tag="PK502_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00246.1"
                     /translation="MKRITAIMVVFLLAALGLADQAQAQRYRKKEISLISFKLYVDPD
                     YRAKLESYSNLFPEPQASSKVNKIDNLILYTSWDIIKRRLEGEVGMIILPIDAYGDKF
                     SYDMYGFPDISINKALSKGRSKYYIKLDLELVPEHSAKYTTQNHVDSARKIVQLKEDE
                     IKPKVVAKLTIYSDKGVIPVANCVGEATTKSIITLSSSFFDGVVNSSIDPSAETLYNL
                     IDKASQQLIISVYENK"
     gene            complement(12720..15944)
                     /locus_tag="PK502_05205"
     CDS             complement(12720..15944)
                     /locus_tag="PK502_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPH00247.1"
                     /translation="MSRLVVYRASAGSGKTFRLAAEYIRMVVTNPYAYRIILAVTFTN
                     KATTEMKERILSELNQLAGGASTPMLEVIISESKLSETTIRQNAKSVLHAILHDYSMF
                     SVSTIDSFVQRVIQNLLWEVGFHSLGELMLEHDAILDRAVDRLLDSTNGDSELFTHLK
                     SMIFYQLDQGKSPDIKKSLVELGNLLYSEQYRLLTDEERAKMGDKSLLVNITTQATER
                     LNAIVQTICSMGKALMQEISSNGFTVDDFKNKSGGVYGIIEKAANFQLGLSYIEVGKR
                     AGDASRDNSVNQWMSMEFIKANPGKSSELQHLVQQSLQPGLARLVEYIEDNRSMFNTT
                     TALRSNLNRLYIVNEIRRYIKEIQAEDNVMVLADSGPLLREFIGENDAPFVYEKIGTR
                     YDSFMLDEFQDTSVIQWQNFKPLVGNSLAQDGFSMVVGDVKQAIYRWRNSDWRILASQ
                     LEEDFEVEPRNLSVNFRSLPGIVNFNNQFFGKAATWLKGWVNRLFEDNPPEDLLKVVE
                     KLYGGAAQESKFHEAIGGLVEVSCLQNIKAGRYDEFLKRKFSMLIPDILKRGYRPGDV
                     AILVRKHSEGKKIADMLLSLKNENPEWADSFNLVSQDALLLDSSHAIRLFISALKLIL
                     NTNDGVSRGVFCKESMVVAKISTSWEHTFVGSDIDDEIAWLTGLRNLPLSTIFEEVLL
                     RYNLQELKQELPYIALLNEQILNYSHGNLASVAGFLEWWDDRGHEVSLSLPDSADAIN
                     ILTFHKSKGLEFPVVIIPYASNSIFKNDKEKALWVSSATEPYCDYPKFLIGMADYLKD
                     SDFRDDYLAEKFQTMVDNLNLLYVAFTRPQNELYVMLSHQADEPKTVQDTGDIILSIV
                     KDMDAPEFTDDRFADISALAVYQFGLVAPIPKTSESKVENRPDVWLMEQYPVGKKIPS
                     LAGHLEASDFFKDEELTVTSGVEHGRIMHRLFSMIRGIGDVSMAVSRLVAEGLISLQQ
                     APALEQHVSEAIQNDAVADWFSEKWQELKVEASILTPTGQTYRPDRVMVSNGHAVVVD
                     FKFGEQQNAHKKQVAGYSELLSQMGYPNVTGYLWYFDSGSIVRV"
BASE COUNT         4598 a         3675 c         3421 g         4413 t
ORIGIN      
        1 atacacaatg caattggggt atgggctgct ggggagttaa catccagctg cgttatccgc
       61 cgttaaagat tagttaaagg tattaaaaat tccgtatcag caatatcaac cacagtgaca
      121 ggaactgctt attatgccca gttctttatt tttcgttatt ataaaattca actgtcgata
      181 ataattcatt cgtaaagttg aagatatcat cgagcgattg caattcgtgt tttgtctctt
      241 ttttctcttt atcgaataat acaatgtatt ttttcgattg accatttagc cataatctac
      301 acactggttt gcgattatta tcgtccaata ttacgctaaa atatgtttgc gaatccctaa
      361 agaatattcg agtagaatca attttagttc taagaatact tttaacaatg taaaaactat
      421 taatttcatc ttccgttgta attaatttgt tttcgtccga ttcttcttca ttttcttttg
      481 ctttcgtact gtcttcagta ggttttgttt gattaattgc attcataagc ctgtcgctta
      541 cggtgtcatt gaatgtttgc tcaaccgttt tcttaacaat ttcagtaaac tgaatcatta
      601 ctttttctgt tgccttcccg tcataaactc tgctcacgaa aaacttcaca aattctggag
      661 atggatttgc aagttgagag tttaaaattg tttttattgc gtttgaatat tttaattcgc
      721 ttgcagtgtt caagattgct ccaatatcaa atccattctt atgaaacttg ataagttctt
      781 taatttgttg gtcttttatt ttcgtgatgt caaaactaaa aaatggtttc tcatccattt
      841 tgttggcaga atcgaggtca gtgtaaaata gatactgaac accatttgtt aacaatgcaa
      901 atcttgcatt agttaagtgg aaatacttgt gtaattgagt ataatgcttt tctaggtttt
      961 cagaatgatt cttgcactca attattaaca caggttgatt ttcctttaaa attgcataat
     1021 caactttttc aacgttttta gcgccatagt cagttacaaa ttcgggtaca acctacattg
     1081 gattaaaaac gtcgtatcca agaacttgaa tgaaggggag aacgaatgca ctttttgttg
     1141 cttcttctgt taggatttgg tctcttagtt ttaaaacctt ttctcctaat tgttttattt
     1201 ggtctttaaa ttccataact gaaagttttt atgttattaa tcgaaagacg tctgtttcat
     1261 caatctaaaa ttgggcataa cggttccgtg gttacaacta caggggattt agtagccagc
     1321 tccgtcatcc cacgttataa ttttaccaaa tatacaaaat aattccataa ccacatctgc
     1381 cccattgcct taaaccacgt gttgtgtgca gctattttag tcattagcaa aatcatctaa
     1441 ttttgtaagt aaatcattga aatcagacgt catataagaa gcatagtttt ctaagaatgg
     1501 gcttttactc ccgctaaagt agacaaaaac aggtttttga ttattgtggg aatagataat
     1561 ttcagctaat actcctggtg acaacttatc ggtcgggtaa attacaactg tagcatctga
     1621 ttggtcaata aacctaaaat ctcgttcaat tgtcctagct ttaataattt tctttgctgt
     1681 atcagtcaca tcagcaatag tgttgggtaa ttcaatttta ttaccgtaag tcaaagacat
     1741 atcttttatt gctaaagggt tgaatactac aaatagtttt tcaagttttt cgagaaattc
     1801 tgtttgaatt tgagttaaca attcaggatt tgaatctttt attgctgtaa ttggataact
     1861 taaatagatt tttttcttgt tcgtataaaa gaaatcaaat agattgtgaa tattatgatt
     1921 tctagcaatt atataaacgg gtttattaaa gaaatcagct atcacttgtg taacaaattc
     1981 ttcttcaatc atccaatttt gtgtttcttc aagatttgga agtgtgtcat tatcccattt
     2041 tggatttatt gaatttattc taaaaacttc ttttacatca ttaactatat ttatgaaacc
     2101 ttcaatctta aatctggaaa gatttctgaa tgataacccc tctatgattc tatgtttcca
     2161 tctaaaagta gcatgaatgc caattaggtg taattcaact tcaggatttt ttaacttaag
     2221 tatttcgatt tcttttaatg cattttcacg taatgtactt aacaaatttc tatcaatgtc
     2281 aagcactcgg ttatcagtca attgaatatc aagtttactt gcttcattat agattaatct
     2341 tccaacatca tgaacttgag tttttcttcc tctatcattt gcatattttt ctaactcatt
     2401 tagtaattca attcttccag aaccagaaat accagttatt atccattttt tcatagtata
     2461 ttgggttatt aggatgtctt catatagtta cacccaactc atttataccc gccactctac
     2521 agcgtctaac ttccacattt ggatgtataa acgctataat gtggtgtctt acctttaaac
     2581 tcaaaagtaa tataaaatct catttaataa taaaccaaac acatttactt attaacaact
     2641 ctccagatgt gccgctggct ttagttaaac ttttgcttac gtgggtttca actttttgtg
     2701 gtcttttaaa tcattggcaa tgaatgattt ttgaatgcac cgcaaatcgg ctaccgctta
     2761 aaccatcctg gtggcgcagc tgctacctag acagggcagg atggccgata atgaaatgct
     2821 aagatccgcc taaaagtgag cggtttttcc agcagtcaac aggattgcca catggaaagg
     2881 agtgtccatg cttcatgaca ttccaaatag ccgcatgggt cgttactatt acacggcatt
     2941 tctattgatt attgttttta acgtccggtt caccctgcta gcaggctgaa gcttgtttgt
     3001 taattttcgg cttaagttaa aacctaagcc tagcaagggc ataagccgaa cgatgggcac
     3061 ccacctcttt cacatgcccg cctgcaaaat tagctgtcat gtaaaaagta attcgacatt
     3121 tagaatacgc ccaagggctt atttttatac ctttaagccg gcgataattg tggaggttac
     3181 cgtaacaacc tgctacgggt attcaggcat acatgttgac tttaaaaaat taagctatga
     3241 caggaattgt tctggttgga attctggtgg tgttggctgt ggtgggcata tccatactgc
     3301 tgattaggaa gcccacagtg gatattaagc cccagctgcg tgaggttgaa aatttgatgg
     3361 tgggttttgg cgccaccctc gaccgagtag agaaatccat aaaggacgaa tttcagcgga
     3421 accgcgcgga aaccaacgaa atttcaaaaa ccaaccggga ggagcttacc aaatcgctcg
     3481 ataccttcag cagcactttt gaaaaaagcg ttaaggagct taacgacgtg cttaaagcca
     3541 agtatgatga gctacggaac cagcaggccg atttcaacag gcagatttcc gatgccaaca
     3601 aggccaaccg cgaggagctg gcaaacagcc ttaaatcgtt cgagacaaaa tttaccgaga
     3661 acatccggga gctgaacgag ctgcttcgtc aaaaattcag cgacttttcg cagcagcaga
     3721 ccgaggtgaa ccgccagctg acggaaaaca taaaggacgt gaagcaatcg gttgataacc
     3781 agctgaaatc cattcgtgag gacaacaccc accagctcga cgagatgcgg aaaacagttg
     3841 atgagaagct gcagaaaaca cttaacgaca ggcttagcca gtcattcgaa acggttggca
     3901 agcagcttca agccgtacag gtggggctgg gcgaaatgaa aaaccttgcg gccgatgtgg
     3961 ggggactaaa acgggtgcta agcaacgtga aaatgcgtgg gggaatcggt gaaatccagc
     4021 tctccatgct gctggagcag attcttgcac ccgaccagta cgaagccaac gttaaaacca
     4081 aacagaacag cgcagacctg gtggaatttg ccattaagct gcccggaaaa gacgactccg
     4141 ggaaaaacgt atggctacct attgatgcca agttccctaa ggacatctac gagcagcttc
     4201 aggatgccta cgatgccggc gaccagaacc tcattctgaa cgcccagaaa aatctggaca
     4261 gcaccattaa aaagatggca aaggatatca gcgacaagta cctcgaccca cccgatacaa
     4321 ccgattttgg catcatgttc ctgccatttg aaggaatcta cgccgaggtt gttcgcaagg
     4381 ccgccctgct cgaagacctt caaagaaact acaaggtgat tgtaaccgga cccaccaccc
     4441 tggctgccat tctcaacagc ctgcagattg gcttcaaaac tctggccatt caaaagcgta
     4501 gcagcgaggt ttggcaggtg ctgggcgctg ttaagaggga atttgagaat ttcggcgggc
     4561 tgatggagaa agcccaaaag aacatccaaa ccggtctgaa ccagctggac gacgtgatgg
     4621 gcaagcgcac ccgcgccatc cagaaaaaac tgaaaagtgt ggaggcgcta agcgaggccg
     4681 aagccaaaag catacttccc gaaatatccg aatcggctat tgttgatgat gaggatgacg
     4741 atttgggtga ggtatagctt tagctcttat ttaaccttaa ttcgaaaaaa ccaaataatc
     4801 cagggtgcaa cattgaacaa caacaatgca acaacctaaa ataacaagcc atgctaaaag
     4861 caaaaatttt gatgggaagc ctgctgattg cagcttctct ggctggaatt tcacaggagg
     4921 ttcaaatgga caatagcaga gtaaagcagc tcacgctgaa caacggttta accgtaatac
     4981 ttgacgagaa tcacgctcaa cccaaggttt ttggattggt tatggtaaag gcgggcggta
     5041 agaatgaccc tgccgatgcc accggaatgg cacactacca ggagcatatg ctctttaagg
     5101 gcactcaaac ccttggcacc atcaactgga aagaggaaaa accttacatt gataaaattt
     5161 ttcaactcta cgactccctg ggtaaaactt ccgatgaagc taagcgcaag caaattcagc
     5221 aggaaataaa cgaggcatcg ctcaaggcaa acagcttcgc aattcccaac gagtttagca
     5281 acctggtgaa cgaaataggc ggttcgatgc tcaatgccgg caccggcccc gactacaccg
     5341 ttttctacaa catgttccct tcaagccaga ttgaacgctg gctcgacctt tacgcccatc
     5401 gttttgttga acctgttttc aggggttttc aggcagagct ggaggtggtt tacgaggaaa
     5461 agaatctata caacgaccag tttttcacca agctgctgga ggagttccaa aagcagttct
     5521 ttaagaatca cccctacgga cagcaaacgc tcattggctc cattgaggat ttgaagaacc
     5581 cctcgctcac caagatgtac gattttttta aaacctacta tgtccccaac aacatggcac
     5641 ttgttctctc cggcgatttc aactccgacg agattattcc cgttatcgaa gaaaaatttg
     5701 gcaaatggaa ggagcatgag ctaccagaaa gtaagatttg gacagaaaaa ccatttaacg
     5761 gaagggagtt ctaccaggcc aagctttccc cggtgaaaat ggggcttctg ggattcagag
     5821 ctccgtcggc taaagacgat ggtttcctgg cagcagaggt ggctacccgc attctcaaca
     5881 acagctactc caccggattg ctcgacaagc taacgctcga tggaaagctg ctggccgctc
     5941 aagctttgca aatgccctac ctcgaccatg gcgccgttat tgtggttatg cttccaaaga
     6001 tacttggcca aagccttgag gatgctgaat cactggttaa taatgaactt ggtaaactta
     6061 aacgaggcga atttgacgac tggatgctgg aggctgttaa gctcgacctc taccgcaacc
     6121 acgttaccac catggagaaa attgatgggc gtgcgctcta cctgggagaa gcttttgcca
     6181 aagggcaatc cgctcaggaa gcgctcgact accctaataa aataatgagg cttaccaagg
     6241 atgatgtggt tgctgcagct aaccaaatct tcggagatag ctatctggcc ttctactcta
     6301 aaatgggctt ccccaaaaag gaaaaaatag aaaaaccaga ctttaaaccc ctgttggcta
     6361 acaccgaagc taaaagcgag tacgctaagc atttcttcaa cctaccatcc aaaaagcctg
     6421 atttcaaccc cattgtattt gatagggatg ttgaaacagt tgaaataggc aatgggcata
     6481 agctgatgtg cgttcagaat cccgccaacg atattttttc cctaacaata aacttcatga
     6541 taggggaaaa caccatgccc ctgcttcgcc tggcctcaca gggaatgggc cttagcggcg
     6601 ccggcagcta taacgtaacc gagttgaaat cggagtttgc ccgaattggc gcatcgtaca
     6661 acatcacctc aaatgattac agcgtgtcta ttgaactaca gggagtggaa agtgaacttg
     6721 accgagcgct cgagcttgta ggaatgctgg taggcgaccc ccgccttgag cagtcgaaaa
     6781 ttaaaacaat agttgacggg gagactaccg ctcgaaaagt tgaacgttca cagcccgaca
     6841 atgtagctca ggcgctactg gagtacaccc tttatggcga tagttcaaac tacctaaacc
     6901 gcctatcgtc gaagcagctt aagaacttga aggccgaaga actggtatca acctttaaaa
     6961 aagcaacgga gtacaacact caaatttact actgcggaaa gacaggcgca tcaaatgtag
     7021 ccgcagaggt gagcaagcat gtcccattgg ctgccaaccc aaaacacgat accaactcaa
     7081 aggacaggct ggccaagcat tacaatgaaa acaccatcta cctggttgat aagccaaagg
     7141 ccagacaaag caaggtgttt gtatttatca acggccaaag ctttacacct gcagatgcag
     7201 ctgtaataga tgcttttaac gactacttcg gaggagggtt ctctggactt attcttcagg
     7261 aaataaggga ataccgctcg ttggcctact ctgccggagg aaaatttaag ttgccccgcc
     7321 aaaaaggaaa tccagctgat tttataggct acgttggaac acaatccgat aaaaccctga
     7381 cggccatgga aacattcaac gggctgatgc gccagatgcc cgaaaagccg gaacgtatgg
     7441 gccttataag aagccacctc gaacttttgg cgcaaaccga ccgaccaaac ttcagggagc
     7501 tggcctcaag ggttgacgag tggcgtgacc agggttttga ggccgaccct gcctttgtta
     7561 agctacctgc ctaccagctg cttaaatggc aggctataga gcaattctat gccgacagga
     7621 tgaagggtaa gccggctgtt tacatgattg ttggcgacaa aaagcaaatc aaccttaagg
     7681 agctgtcatc gtttggaaaa gtgattgaac tcaaggagga taagctgttc aacaaataac
     7741 tttgggagag attactgaaa agtaaaaaaa gaccaatacg cgtattggtc tttttttgtt
     7801 gtgaccccga agggattcaa acccccaacc ttctgatccg tagtcagatg ctctattcag
     7861 ttaagctacg gggccttgtg tttcaagtgc aaaattattc aatatttcca taccatgcaa
     7921 ccatttcctg ctgaaaaaaa ataaaaaacc gtcatttcaa ccagtaaaat ttactcctta
     7981 ccaaagcgtt aaccggctga tttaactaca aatcaggcaa ttatcaaaac aataaaggca
     8041 accacttgca aacgcctgcc tacaacaaat tgcgcattaa acccaaactg atattcagaa
     8101 gggatatggg agctattaaa ttggtttaag catgggtggt taataaaaaa tccccaactt
     8161 aaaggggatt ttatcagctt actctccaat atcgagcttg cgttttttct ccttaatgcg
     8221 tgccttttta cccgtgaggt tgcgcaggta gtatattcgg gctctgcgaa cttttccctc
     8281 cttagccact tcaatttttt cgataaatgg ggagttaagc ggaaaaatcc tttcaacacc
     8341 tacaccacct gaaatctttc ttacggtaaa ggtttcggtt gcccccacgc ctcttctctg
     8401 aattacgacg cccctgtaat tctgaattcg ctccttgcta ccctcaacaa ttttgtagtg
     8461 aacgacaacg gtatcaccac tcttaaagga aggatgctcc tttttagagg caaatgactc
     8521 ttctgcaact ttaatgaggt ccattttagt ttgattttta agtataactg ggaaatgcaa
     8581 tattaccggc acccatgtcc tggtaagaga ttacacttca aattgggcgc aaatttagaa
     8641 aattaatatt aataaacagg taaattaaag ccaataattt gatttttttc atgaaccata
     8701 tgttgaaaca cctctgagta ggcaaaccgc tagcggggat gataagggga gtaaacgctt
     8761 gccctcaacg atttcaacca ggctcatccg aaagatagtc aacatcaaaa acatctaacg
     8821 cgaccttttt gtcaaccggt aaaaatgtgc atttgagggg aaagaaaatg cggaggaaaa
     8881 attttcctcc gcatagctag gtggctcaat aatactagtg cccccctttg ttttcaggta
     8941 caagaagttt atcgccggta gcatcaatta ctgctttctt ccaccagtcg gggtattcag
     9001 gattgcttac ccgtgctgga cactggtggt actcgttgta aataaactga ccattctcgt
     9061 caacctgctt cacattccca tccatgtatt tcagcagcag gaactggaaa agcttttccc
     9121 aacgcgtaac aacgtagtcg ccagtattaa cggagtagtc ggtaaggaac ctgattgcct
     9181 cggattggtt ttgctcgtag agcttaagag cggtagcatc tacaacctgg gtgttgctaa
     9241 taaacctgcc ctccagctcg tcctgcacct ttttaatatc ctggctcatc aggtcgtacc
     9301 taaggtaggc gaagttggaa accttattaa aagtccagaa agcacaatgg tcgcagtagg
     9361 tgagcatgtc gcccttaccc tcggcgtacg attcgggaac agctgttata ccgcagtatg
     9421 ccgggaaata aacggttgag gccacatcgt caacgctgaa ccagaaaata ccaccaattg
     9481 ggtcgggcag ccagttccga gcctgtgcaa caaacgagaa gccagtttgc tgggtggcgg
     9541 ttgcgcgttc gttaacgtac tccttaccct cgtatttcca ctccatagga cgccagcgat
     9601 atggcatggc atgagggcca gctccaggat ccttccgcat gtcgagctcc gttccctcca
     9661 ggtggtcgcg catgaagttc atcatgtcct taacggaaat cttcctttcg ggcttgatgt
     9721 agagcggcat acgatgtttt aggttatgcc ctttggcata gtcgaagtaa tcccacatgg
     9781 actggtttac ctccttaaaa aacgaccata cccgaatctc gcaagcacgg gcagcaccaa
     9841 aatcgagcgg agcataggta tcggaaaagc taaaatcctc atttgtgccg gtatagtatc
     9901 ccttggtacg ggcgaagtca atgacatcag aagagtaaac cacctcaaca cccggatcga
     9961 aaatcttgtc aaggttcctg gagcttatag aatttttcag attttcttgt gggaaggtgg
    10021 tgatgcgggc ctggttggca tgtcccgaga tgtagccgtc gggaatacga atggctaccc
    10081 aaacagctcc cttatcggca ttgtaggatt tctttgtttt cttgtcaacc ttcaggttag
    10141 tcccctttcc aatcatttca aaaatccata cctcattttt atcagccaca gagaacgatt
    10201 ccccctcgct gtagtacccg tactgctcca ccagctctgc cataaccttg atggcttcac
    10261 gagcgctctt ggaacgttgc agggcaatgt acatcagcga tccatagtcg attaatgctg
    10321 tgctgtcctg aagctccagg cgccccccgt aggtggtttc accaatggat acctgatact
    10381 cgttcatgtt tcctaccacc gagtaggtgt ggccaacctg gggaatttca cccaaatact
    10441 taccggtgtc ccactcgtat attttcatca ttgtgcctgc agggtaatcg gccgcaggac
    10501 ggaagtaaag ctcgccaaac aggtagtggg aatcggcaga gtaggtaata aaggttgacc
    10561 cgtcggttga ggcacccctg gttaccaaaa agttagtgca cgcccacccc gtatcagcaa
    10621 gaaaagcaaa ggataccaga gtaaaaattt gaacagcaag ttttctcata gcttgtcatt
    10681 ttaagaatta gcaaattgca aacctagcat ttatttcgat ttttctgcat gatgaaggtt
    10741 taatcaacca tattcttcac catcagaatt ataggtatag acatccggaa atggaatcgg
    10801 cagtagcacc ggttgatgag gcaagcgccg aaggggtacc gttaaggaaa agggctgcaa
    10861 gaaaggcaaa aatcaaagct tccttaaaat caaccagcat gttttctgga actaccacgc
    10921 gatgtttcac tttttctgta atgagctgca caagaaatct attcctggcg ccaccgccgg
    10981 taaccagcat agcacctgga ctaatttctg ctgttgattt agaaatctga atggcaatat
    11041 gctcgtaggc tgtacgtagc ttatcctgaa gggatgcatt gcagcttgca aaaacaggaa
    11101 ctaccttttg ctcaaaccac tccctaccga gtgatttcgg cccgagcaac ccgtaaaact
    11161 ccagctcatt aagccggttg agcaggtcat ggttaaccat cccttttgca ccagcattgc
    11221 cataagcatc atactccatt cccagctgga gcgaaaggaa gttgagcagc atgttgcatg
    11281 gtgcaacgtc gcaggccacc ctttcgttgt caacattgtc gaagcttatg ttggctattc
    11341 ctccaaggtt caggcagatg tcataactgg tgaaaagcag cttatcacca atgggaacaa
    11401 ggggtgcacc ctgtccattt cgacaaatgt cagcagcgcg aaaatcggaa atgcacggaa
    11461 tgcccgtttc tgcaactata gcagcaccct tgccaatttg aaaggtatag cccatgccag
    11521 gattgtgaaa agcagtatgc ccatgcgagg caaccacatc ggcatggcgg tttatttgct
    11581 tcaggaaact aaaaacctcc ttcccaataa agtggccata atcgtaatcg agctttgata
    11641 tttcccacgc tgtcagcttt tcaatatcac ttagcttgat ttttacttca gcactataag
    11701 ggacggtatt ggctgcttca atggtgtagg cccacgaatc tgactttttt tcgaaatgag
    11761 ctaggcaaat atccaaacca tcaagcgagg ttccagacat tacaccaact acgctcaacg
    11821 gatttttgtt gaagagcttt accatcactt gttctcgtaa acgctgatga tcagctgctg
    11881 cgaggcctta tcaatcaagt tgtaaagcgt ttcagcagaa ggatcgatgc tgctgttcac
    11941 cacgccgtcg aaaaaggagc tgctaagggt aataatgctt tttgttgtag cctccccaac
    12001 acaatttgcc accggtataa ctcccttatc ggagtatatg gtaagctttg caactacttt
    12061 tggtttaatc tcatcctctt taagctgaac gattttccta gcagaatcaa cgtgattttg
    12121 ggtggtgtac ttagcagagt gctccggaac taattccaaa tctagcttaa tatagtactt
    12181 tgatcttccc tttgaaaggg ctttatttat actaatatcc ggaaatccgt acatatcgta
    12241 ggaaaactta tcaccatagg catctatggg aagtataatc atacctacct caccctcgag
    12301 ccttcttttt attatatccc acgaagtgta aagtataagg ttgtcaatct tgtttacctt
    12361 actgctagcc tgaggctcgg ggaacaggtt gctataactt tcaagttttg cccgatagtc
    12421 ggggtcaacg tatagcttga atgagattag cgaaatttcc tttttgcggt atcgctgcgc
    12481 ttgcgcctgg tcagccaaac ctagagctgc cagtaaaaac acgaccatta tagctgtaat
    12541 tcttttcatc ccccaatcaa tttataaatt ccggaaaaaa ctctacgagc aaagatagtc
    12601 aatttttcaa acgagctccg ggttcaataa attgtacttt tgctacaaat ttcacgtttg
    12661 tagcggggtt ctcaattgct tcagcataaa agtggcagtg agctgccagc agttagcttt
    12721 tacacccgga ctatggaacc ggaatcgaag taccaaaggt aacctgtcac attggggtaa
    12781 cccatttgcg acaggagttc cgaataccct gccacctgct tcttgtgggc attctgctgc
    12841 tcgccaaatt taaagtcaac taccacggca tggccattgc taaccatcac ccggtcgggg
    12901 cgataggttt gcccggttgg ggtgaggatg gaagcctcaa ctttcaactc ttgccatttt
    12961 tctgaaaacc agtcagctac agcatcattc tgaatagctt cgctgacatg ctgttccaac
    13021 gcaggagctt gctggagaga aatcaggcct tctgcaacaa gtcggctgac ggccatactc
    13081 acatcaccta ttcccctgat cattgaaaac aggcggtgca taattctccc gtgctctact
    13141 cctgaggtaa cggttaattc ttcatcctta aaaaaatcgg atgcctcaag gtgtcctgcc
    13201 agggaaggaa ttttttttcc aacagggtac tgctccatca gccaaacatc ggggcgattc
    13261 tctaccttac tctcggatgt ttttggtatt ggagcaacca gcccaaactg gtaaacagct
    13321 aatgctgaaa tatcggcaaa cctgtcatcg gtaaattcag gggcatccat atctttaaca
    13381 atggaaagaa tgatgtctcc ggtatcctgc accgttttgg gttcatcagc ctggtggcta
    13441 agcatgacgt aaagttcgtt ctgaggtctt gtaaaggcta cgtagagcag gtttaggtta
    13501 tccaccatgg tttgaaactt ctcggcaagg tagtcgtctc tgaaatcgct atccttaagg
    13561 tagtcggcca tgcctatcag gaatttcggg taatcgcagt atggttcagt agcagacgac
    13621 acccataatg ctttctcctt gtcgttctta aagatgctat tgctggcgta ggggataatc
    13681 acaaccggaa actcaagccc cttggactta tgaaaagtta aaatgttgat tgcatcagcc
    13741 gagtcgggaa gcgagaggga aacctcgtgt ccacggtcat cccaccactc caggaagcca
    13801 gcaactgaag ccaagttccc gtgagagtag ttcaaaatct gctcgttgag cagggctata
    13861 tagggcaact cctgcttcaa ctcctgcaaa ttatagcgaa gtaaaacctc ctcaaaaatg
    13921 gttgaaagtg gaagatttct aaggccagta agccaggcaa tctcgtcatc aatatcgctt
    13981 ccaacgaagg tatgctccca gctggttgaa attttagcta ccaccatgct ctccttgcag
    14041 aaaacgcccc ttgacacgcc atcgttggtg tttaatataa gcttaagggc cgaaataaag
    14101 agcctaatag catgtgatga gtcgagtagc agggcatcct ggcttaccag gttaaaggag
    14161 tccgcccatt caggattttc gtttttgagc gatagcaaca tgtcggcaat ctttttgccc
    14221 tcgctgtgtt tcctaaccag gatggctaca tctccgggtc tgtacccacg tttcaagatg
    14281 tcgggtatca gcattgagaa cttgcgtttt agaaactcat catacctacc tgctttaata
    14341 ttctgtaagc acgatacctc cacaagtcca cctatggctt catgaaattt gctttcctgt
    14401 gctgcacctc catacagctt ttcgactact ttcaaaaggt cttcgggagg attatcctca
    14461 aagagccgat taacccaacc cttcaaccat gttgcagctt taccaaagaa ttggttgtta
    14521 aagttcacaa tcccgggaag actgcgaaag tttacggaca aattgcgggg ttccacctca
    14581 aagtcttcct caagctgcga ggccagtatt cgccaatcgc tgttacgcca gcggtaaatt
    14641 gcctgcttca catcgccaac caccatgcta aaaccatcct gagccaagct attgccaacc
    14701 agaggtttaa agttttgcca ctgaataacc gaggtatcct gaaactcatc gagcataaag
    14761 ctatcgtagc gggtaccgat tttctcataa acaaacggtg cgtcgttctc cccaataaac
    14821 tcacgaagca atggtccgct atcggctagc accatgacgt tatcctctgc ctgaatctcc
    14881 ttgatgtacc ttcgtatctc attgactatg tagagcctat tcagattgga acgtagcgcc
    14941 gtggttgtgt tgaacattga gcggttgtcc tcaatgtact caaccagcct ggccaacccg
    15001 ggctgtaacg attgttgaac taagtgctgc aactccgatg atttgcctgg atttgccttg
    15061 atgaactcca tgctcatcca ttgatttacg gagttatccc gtgaggcatc tccggccctt
    15121 ttaccaacct caatgtagct caaccctagt tgaaaattcg cagctttttc aatgattcca
    15181 taaacgccgc ccgacttatt cttaaaatca tcaacagtaa agccgttgct gctgatctcc
    15241 tgcatcaagg ccttgcccat gctgcaaatt gtttgaacta tggcatttaa cctctccgtg
    15301 gcctgagtgg taatgttgac cagcaacgat ttatcgccca ttttggctct ttcctcgtcg
    15361 gtaagcaacc ggtactgctc ggagtaaagc aggttgccca gctctaccaa acttttctta
    15421 atatcagggc tttttccctg atcgagctga tagaagatca tggattttaa gtgagtaaat
    15481 agctctgaat ctccgttagt gctgtcgagc agcctgtcaa cagccctgtc gagaatagca
    15541 tcatgctcaa gcatgagctc gcccaagcta tgaaagccta cctcccagag caggttttga
    15601 atgacacgct gaacaaagct gtcgatggtg ctaacgctga acatggagta gtcgtggagg
    15661 atagcatgca gaaccgattt ggcattttgg cgtatggtgg tttcggacaa tttagattct
    15721 gaaataataa cctcgagcat cggggtgctg gcaccaccag ccagctggtt caactccgaa
    15781 agtatgcgct ccttcatttc ggtggttgcc ttgttggtaa atgttaccgc tagtataatc
    15841 ctgtaggcat aagggttagt taccaccatg cggatgtact cggcagccag cctgaaagtc
    15901 tttccagaac cagcggatgc cctataaaca acaagcctac tcatagcaac agctaaaaat
    15961 caatcaactc taaaaatagg aaaaatctga ataaatccac agggtgggca gaatgttgtt
    16021 ggaattataa gtgggtgtta aaggataaag gttaaaggat aaaaaaattc aagcaggatt
    16081 caagaacctg tccgcctact ggcggaa
//
LOCUS       DAOPHX010000049        15851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_4208_length_15851_cov_5.072803, whole genome shotgun sequence.
ACCESSION   DAOPHX010000049 DAOPHX010000000
VERSION     DAOPHX010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 15851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15851
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4208_length_15851_cov_5.072803"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..134
                     /locus_tag="PK502_05210"
     tRNA            63..134
                     /locus_tag="PK502_05210"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:95..97,aa:Thr,seq:ggt)
     gene            196..1383
                     /gene="tuf"
                     /locus_tag="PK502_05215"
     CDS             196..1383
                     /gene="tuf"
                     /locus_tag="PK502_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPH00248.1"
                     /translation="MAKEKFDRSKPHVNVGTIGHVDHGKTTLTAAITKILAEKGLSEY
                     RSFDSIDNAPEENERGITINTAHVEYQTEKRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVNVPKIVVFLNKCDAVDDPEMLDLVEMEVRELLTF
                     YQFDGENTPVIRGSALGALNGEPKWVEKVMELMAAVDEYIPIPPRENEKPFLMPVEDV
                     FSITGRGTVATGRIETGVIHTSDEVEIIGLSHEKMKSVVTGVEMFRKILDQGEAGDNV
                     GLLLRGIDKKEIKRGMVVAKPGSITPHTKFKAEIYVLKKEEGGRHTPFHNHYRPQFYL
                     RTLDVTGEIVLPEGVEMVMPGDNVTITVELIYPVAINKNLRFAIREGGRTVGAGQVID
                     IIE"
     gene            1430..1502
                     /locus_tag="PK502_05220"
     tRNA            1430..1502
                     /locus_tag="PK502_05220"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1463..1465,aa:Trp,seq:cca)
     gene            1519..1710
                     /gene="secE"
                     /locus_tag="PK502_05225"
     CDS             1519..1710
                     /gene="secE"
                     /locus_tag="PK502_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPH00249.1"
                     /translation="MKITKYFHEVYTEMVHKVSWPTMKELQSSALIVIVASLLIALVV
                     FVMDISFENLMKVIYRMLN"
     gene            1734..2282
                     /gene="nusG"
                     /locus_tag="PK502_05230"
     CDS             1734..2282
                     /gene="nusG"
                     /locus_tag="PK502_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPH00250.1"
                     /translation="MEENVKKWYVLRAISGKEKKVKEYLENEITRLKLQDFISQVLIP
                     TEKVYQIHNGKKISKERIFYPGYVLVEAALVGEVPHILRNAPNVLGFLGDPKSGDPIP
                     LRTSEVNRILGKVDELVDSEEELNVPFFVGETVKVIDGPFNSFSGVIEEVNDEKKKLK
                     VMVKIFGRKTPLELSFMQVEKE"
     gene            2306..2746
                     /gene="rplK"
                     /locus_tag="PK502_05235"
     CDS             2306..2746
                     /gene="rplK"
                     /locus_tag="PK502_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006045676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPH00251.1"
                     /translation="MAKEIAGFIKLQVKGGAANPSPPIGPALGSKGVNIMEFCKQFNA
                     RTQAQAGKVLPVIITVYNDKSFDFVVKTPPVPVQLLEITKQQKGSAEPNRKKVASVTW
                     EQVRQVAQDKMPDLNCFTLESAMKMVAGTARSMGIAVTGEFPAK"
     gene            2768..3469
                     /gene="rplA"
                     /locus_tag="PK502_05240"
     CDS             2768..3469
                     /gene="rplA"
                     /locus_tag="PK502_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPH00252.1"
                     /translation="MTKLTKNRKLALSKVEPNRQYKLSEAAALLKDITYTKFDASVDI
                     DVRLNVDPRKANQMVRGVVTLPHGTGKQVRVLVLCTPDKEEAARQAGAEFVGLDEYIE
                     KIKGGWTDVDVIITMPTVMGKIGALGRILGPRGLMPNPKSGTVTMDVDKAVKEVKQGK
                     IDFKVDKFGIIHASIGKVSFEPEKIVDNAIEFLNTIIKLKPTTAKGTYLKSIYLSTTM
                     SPGLAIDTKSIAAAN"
     gene            3497..4021
                     /gene="rplJ"
                     /locus_tag="PK502_05245"
     CDS             3497..4021
                     /gene="rplJ"
                     /locus_tag="PK502_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPH00253.1"
                     /translation="MTREEKKNLIDALTETINRYPHFYLTDTSALNAEDTSKLRRSCF
                     QKEVKMVVVKNTLFKKALEQSGKFDFSPLFTELKGTTSVMFTESGNVPAKLIKEFRTE
                     HPKPLLKAAFVEESIYVGDEQLNMLASLKSKNELIADVIALLQSPAKNVIASLQSGGN
                     ILSGVVKTLSEKEK"
     gene            4056..4433
                     /gene="rplL"
                     /locus_tag="PK502_05250"
     CDS             4056..4433
                     /gene="rplL"
                     /locus_tag="PK502_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPH00254.1"
                     /translation="MADLKKFAEDLVNLSVKEVNELAKILKDEYGIEPAAAAVAVAAA
                     PAAGAGAAAAEKTQFDVILKAAGGAKLQVVKLVKDLTGLGLKEAKDLVDAAPKPVKEG
                     VSKEEAESLKAQLEDAGAEVELK"
     gene            4528..8352
                     /gene="rpoB"
                     /locus_tag="PK502_05255"
     CDS             4528..8352
                     /gene="rpoB"
                     /locus_tag="PK502_05255"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPH00255.1"
                     /translation="MSNNNNNNRISFSRTKSQYEYPDFLEVQLKSFTEFFQLGTSPEE
                     RRHEGLYKVFLENFPITDTRNNFVLEFLDYFVDPPRYSIEECLDRGLTFCVPLKAKLK
                     LYCTDPEHEDFDTVVQDVYLGTIPYMTPRGTFVINGAERVVVSQLHRSPGVFFGQSIH
                     PNGTKLYSARIIPFKGSWIEFATDINNVMYAYIDRKKKLPVTTLLRAIGYESDKDILE
                     IFNLAEEVKVNKINLKKIVGQRLAARVLKSWVEDFVDEDTGEVVSIERNEVILDRETV
                     VENEHIDTIIESGAKSILVHKENQTFSDYAIIYNTLQKDPCNSEKEAVVYIYRQLRNS
                     EPPDEATARDVIDKLFFSDKRYDLGEVGRYRINKKLNLTTSPDVKVLTREDIIEIIKY
                     LIELVNSKAEVDDIDHLSNRRVRTVGEQLAAQFSVGLARMARTIRERMNVRDNEVFTP
                     IDLINSKTLSSVINSFFGTNQLSQFMDQTNPLAEMTHKRRLSALGPGGLSRERAGFEV
                     RDVHYTHYGRLCPIETPEGPNIGLISSLCVYARINDLGFIETPYRKVDGGTVGMNDDD
                     VVYLTAEEEEAKIIAQANAPIGDDGSFVESKVKARYEGDFPLAESESIDLMDVAPNQI
                     ASIAASLIPFLENDDANRALMGSNMMRQAVPLLKPETPIVGTGLEGKVVSDSRVQVVA
                     EQDGVVEYVDANEIVVRYNRSEDEVFVSFDPELKRYSLPKFKKTNQNTSITLTPIVTK
                     GQIIKKGDILTEGYATRNGELALGRNLKVAFMPWKGYNFEDAIVINERLIRDDMFTSL
                     HIDEYILEVRDTKRGMEELTADIPNVSEEATKNLDENGMIRIGANVVPGDILIGKITP
                     KGESDPTPEEKLLRAIFGDKAGDVKDASLKASPSLYGVVVDKMLFSRAIKDEKDQKKS
                     KSTEKSKLEKIDNEFNRQVAELQTKLVDKLFVLVNGKTSQGVVDNFNQELIPRGVKFT
                     HKMLTEIDYMNVNPNKWTTDKAKNELIKTVIQNYILKWKEIDAEFKRKKFNVTIGDEL
                     PAGIMQLAKVFIAKKRKLKVGDKMAGRHGNKGIVAKVVRDEDMPFLEDGSPVDIVLNP
                     LGVPSRMNLGQIYETVLGWAGQKLGVSFATPIFDGATLDDITVYTEKAGLPRFGKTYL
                     YDGGTGERFDQPATVGVIYMMKLGHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRF
                     GEMEVWALEAFGASHILQEILTIKSDDVVGRAKAYESIVKGEPMPEPGIPESLNVLLH
                     ELRGLGLSINLD"
     gene            8426..12706
                     /gene="rpoC"
                     /locus_tag="PK502_05260"
     CDS             8426..12706
                     /gene="rpoC"
                     /locus_tag="PK502_05260"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPH00256.1"
                     /translation="MSFRKDNKAKSNFSKITISLASPEEILERSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIT
                     LVVPVAHIWYFKSLPNKIGYLLGLPSKKLESIIYYERYVVVNPGIKAADGVKYLDFLT
                     EEEYLEIIDSLPKDNQHLDDNDPNKFIAGMGAEALNLLLSRLNLDELSYDLRNRANTE
                     TSQQRKSEALKRLQVVEAFRESGTINKPEWMILKVIPVIPPELRPLVPLDGGRFATSD
                     LNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRKSNAVKTDANR
                     PLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKMHECGLPKEMAAELYKPF
                     IIRKLIERGIVKTVKSAKKIVDRKDPVVWDILENVLKGHPVLLNRAPTLHRLGIQAFQ
                     PKLIEGKAIQLHPLACTAFNADFDGDQMAVHLPLSNEAILEAQILMLGSHNILNPANG
                     APITVPSQDMVLGLYYITKPRKGVKGEGLIFYSPEEVIIAYNEHAVDLHAIIKVRLML
                     PDKNGQLTPQLVETTVGRVLFNQNVPAEMGYINEVLTKKSLRDIIGAVLKKAGTAKAA
                     KFLDDIKDMGYYMAFKGGLSFNLDDVVIPPEKAQLIEEGYAQVDDVMANYNMGLITNN
                     ERYNQIIDIWTHTNAKLTLTLLKTLSEDNQGFNSIYMMLDSGARGSKEQIRQLGGMRG
                     LMAKPQKSGSVGNEIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAGYLT
                     RRLVDVSQDVIITENDCGTLRGLVATAIKKNEDVVETLYERILGRTTVHDVFHPLTGE
                     LIVAAGEEITEEIASIIEDSPIEQVEIRSVLTCESRKGVCSKCYGRNLATGRMVQIGE
                     AVGVIAAQSIGEPGTQLTLRTFHVGGTASNIISDSKIIARFDGRAEIEELKTITTEDE
                     LGQPYSIVIGRLAEMRIVDLNTGITLSTHNIPYGSKLYISNGDTIKKGQLICEWDPWN
                     AVIVSEVSGRVEFDALIKEVTFREESDEQTGYREKVIIETRDKSKNPAVKITDEKGEG
                     IRMYNLPVSAHVVVDEGTVIRAGDIVAKIPRAMGKSGDITGGLPRVTELFEARNPSNP
                     AIVSEIDGEVTFGKVKRGNREIMVTSKTGEVKKYLVPLSKQILVQENDYVKAGMPLSD
                     GAITPTDILAIKGPTQVQEYIVNEVQEVYRLQGVKINDKHFEVIVRQMMRKVEIDDPG
                     DTRFLEKQVVDKWEFLEENDYIFDKKVVIDPGDAPNYRPGQIITARMLRDENSALKRK
                     DLKPIQARDAIPATSSQVLQGITRAALQTNSFMSAASFQETTKVLNEAAVRGKVDNLE
                     GLKENVICGHLIPAGTGLREFDRIIVYSKDEHEFVEKAGKSVSLTSND"
     gene            13096..13413
                     /locus_tag="PK502_05265"
     CDS             13096..13413
                     /locus_tag="PK502_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HPH00257.1"
                     /translation="MPEKMNPINIEINDEIAQGIYSNLAVITHSSSEFIIDFVRVMPG
                     IPKAQVKSRIVLTPDHAKRLMLALQENIGKFEAANGTIKLPEGSEGMPPFPINFGGKG
                     GVA"
     gene            13602..15671
                     /locus_tag="PK502_05270"
     CDS             13602..15671
                     /locus_tag="PK502_05270"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HPH00258.1"
                     /translation="MKKFADYTLMALLAGMLACSNNNEQLVTSPSGEVKVIFSLTADK
                     QPSYRVYFKDSPVIETSTLGFDFKDAPSLSKGMRVDSVLSNEFNETWELPWGEQRLVK
                     NHYNEMKVFLSEKKSPNRHFAVTYRVYDDGIGFRYEFPQQESMGDVTITDENTQFKLT
                     GDHLCWWEPGDWDIYEHLYNTTSFSKIDATKKRNHRDLAQTYIPENAVNTPVTMKTSQ
                     GLYLSFHEAAVNNYAGVTLKVDTVNYSMTTELVGNDSGVKVVTKTPFNTPWRMIQLAE
                     KPGDLIESRLILNLNEPNKLGDVSWFKPTKYVGIWWEMHLGKSSWDMASGKHGATTAN
                     AKRYIDFTAKNNIGALLVEGWNTGWEHWIGFDDREGVFDFVTPYPDYNLKEVVEYGRK
                     KGVDLIMHHETSSAVTTYDKQLDTAFALISSLNLHMVKTGYVGKIIPKGEYHHGQWMV
                     NHYRRVLEVAAKHKVAVDVHEPFMATGERRTYPNFVSQEGFRGQEFNAWSSDGGNPPE
                     HLAIIPFTCLLAGPMDYTPGIFNTKLKPYKPNNQVNNTLAQQLALYVVIYSPIQMAAD
                     LPENYENHPAFQFIRDVGVNWEQTRVLNGEVGDYVTIARQERGTNRWFIGAVTDENSR
                     ELSVKLDFLEPGKKYSATLYLDAPDAHWDKNPSAYEIKKMEVDSSTELNLYLAPGGGA
                     AISLFPL"
BASE COUNT         4524 a         3444 c         3849 g         4034 t
ORIGIN      
        1 agcatcagcc ttccaagctg agggtcgcgg gttcgagtct cgtctcccgc tctgcacaca
       61 atgccgatgt agctcagggg tagagcactt ccttggtaag gaaggggtca tgagttcaat
      121 tctcatcatc ggctcgattt tttaaacatt tgaaattcaa gtttttaaat actaatttaa
      181 ttaatacctg aagttatggc taaagaaaaa tttgataggt cgaaaccgca cgtaaatgtt
      241 ggaaccattg gccacgttga ccacggcaaa acaactctga ctgccgcaat taccaagata
      301 ttggcagaaa aaggactttc ggagtaccgt tcgtttgact ctattgacaa cgctcccgag
      361 gaaaatgaga ggggtatcac cattaatacc gctcacgttg agtaccaaac cgagaaacgt
      421 cactacgctc acgttgactg ccctggccac gccgactatg ttaagaacat ggtaactggt
      481 gctgcccaga tggatggtgc aattctggtg gttgccgcca ctgatggccc catgccccaa
      541 acccgtgagc acatcctgct tgcccgccag gtaaacgttc ctaaaattgt ggttttcctt
      601 aacaagtgcg atgctgttga cgatcccgaa atgctcgacc ttgttgaaat ggaagttcgc
      661 gagctgctca cattctacca gtttgacgga gaaaatactc ctgttatcag gggttctgca
      721 cttggtgcgc tgaatggcga acccaagtgg gtggaaaagg taatggagct gatggctgcg
      781 gttgacgagt atatccccat tcctccccgt gaaaacgaaa aacccttcct gatgcctgtt
      841 gaagacgtct tctcaattac aggccgtgga actgttgcta ccggacgtat cgaaaccggt
      901 gttatccaca ccagcgatga agttgaaatt atcggtctta gccatgaaaa gatgaaatcg
      961 gttgttaccg gcgtggaaat gttccgcaaa attcttgacc agggtgaagc cggtgataac
     1021 gttggtttgc tccttcgcgg tatagacaaa aaggaaatta agcgcggaat ggtggttgcc
     1081 aaacctgggt ccatcacacc tcacaccaag ttcaaggctg aaatatatgt tttgaagaaa
     1141 gaggagggtg gccgtcatac cccgttccac aaccactacc gtccccagtt ctatcttaga
     1201 actcttgacg ttaccggtga aatagttcta cccgaaggtg tggaaatggt aatgccgggc
     1261 gacaacgtaa ccattaccgt tgagcttatt tatcctgttg ctattaataa aaaccttcgt
     1321 ttcgcaattc gtgaaggtgg cagaacagtt ggtgctggtc aggtaattga tataatcgaa
     1381 taatactgct attctggcaa aaggcattac ctgccttgag ccagcctaca cgggtgtagc
     1441 tcaattggta gagtagtggt ctccaaaacc attggttggg agttcgagtc tctccacccg
     1501 tgctaaatat ctgaagatat gaagattaca aagtactttc acgaagttta caccgaaatg
     1561 gtgcataagg tttcgtggcc tactatgaaa gaacttcaat ccagcgcatt aattgtcatt
     1621 gttgcttccc ttttaattgc gctggtggtt ttcgtcatgg acatcagctt tgagaacctg
     1681 atgaaggtta tctaccgtat gcttaactaa ccagcgccca acttgggaaa ggaatggaag
     1741 aaaatgtaaa aaaatggtat gttcttcggg caatttccgg taaggagaag aaagttaagg
     1801 agtacctgga gaatgagatt acccgtttga aattacagga cttcatctct caagttctta
     1861 tccctaccga aaaggtttac cagattcata acggtaagaa aatcagtaag gagagaattt
     1921 tttaccctgg ttatgttctt gttgaagctg cgctggtggg tgaggtccct cacattttga
     1981 ggaatgcccc caacgtgttg ggatttttag gcgaccccaa atcgggcgat cctatacccc
     2041 ttagaacttc agaggtaaac cgtattcttg gaaaggttga cgagctggtt gatagcgagg
     2101 aggagctgaa tgttccattc tttgttggtg aaacggtgaa ggtgatagac ggtccgttta
     2161 actcgttttc cggagtaatc gaggaggtaa atgacgaaaa gaagaagctg aaggttatgg
     2221 tgaaaatctt cggccgaaaa actccgctgg agttaagttt tatgcaagtt gaaaaagagt
     2281 aactaactaa aagagcaaat gaattatggc taaagaaatt gctggattta ttaagctcca
     2341 ggttaaaggc ggcgctgcta atccatcgcc tccaattggg cctgctcttg gttctaaagg
     2401 ggtaaacatt atggagttct gcaagcagtt taatgccaga actcaagctc aagcagggaa
     2461 agttttgcct gttataatta ctgtttacaa cgataagtca ttcgattttg ttgttaaaac
     2521 cccacctgtt cccgttcagc tcctggaaat taccaagcag cagaagggat cagctgaacc
     2581 aaacaggaag aaagttgctt cagtaacctg ggagcaggta aggcaagtag cccaggacaa
     2641 aatgcccgat ctgaactgct ttacccttga gtcggctatg aaaatggtag caggcacagc
     2701 ccggagcatg gggattgctg ttaccggaga gtttccggct aaataaccac ttaacatctt
     2761 ttgagcaatg actaaactga caaaaaacag gaagttggct ttatcgaagg ttgagccgaa
     2821 caggcagtac aagctttctg aggcagctgc cctgttgaag gatatcacct acacaaagtt
     2881 cgacgcttcg gttgacatcg acgtgaggct taatgtcgat cctcgtaagg ccaaccagat
     2941 ggttcgcggg gtggttacac ttcctcacgg aaccggcaaa caggtgaggg tgctggtgct
     3001 ttgtactccc gataaggagg aagctgctcg tcaggccgga gccgagtttg ttgggcttga
     3061 tgagtatatt gaaaaaatca aaggagggtg gacagatgtt gatgttatca ttaccatgcc
     3121 tacggttatg ggtaaaattg gcgccctggg ccgtatcctt ggacctagag gacttatgcc
     3181 caaccctaaa agcggcactg taaccatgga cgttgataag gctgttaagg aggttaagca
     3241 ggggaaaatc gactttaagg ttgacaagtt tggtataatt cacgcttcaa tcggtaaggt
     3301 ttcttttgag cccgagaaaa tagttgataa tgccattgaa tttcttaata ccattatcaa
     3361 gcttaaaccc acaacggcta aaggaaccta cctgaagagc atttaccttt ccactactat
     3421 gagccccggt ttggcaatcg ataccaaatc tatagctgct gcgaattaat aaaaccgttt
     3481 acagacccaa ttcactatga caagggaaga gaaaaaaaat ctgattgatg cgcttaccga
     3541 aactataaac cgctacccgc atttttacct caccgatacg tcggcgctta atgctgagga
     3601 cactagcaag cttcgccgct cgtgctttca gaaagaggtt aaaatggtgg tggttaaaaa
     3661 taccctgttt aagaaagccc tggaacaaag cggcaaattt gacttttcgc cgctatttac
     3721 agagttaaag ggcaccacat cggtaatgtt taccgaatcg gggaatgtgc cggctaagct
     3781 tatcaaggag ttccgaactg aacatccgaa acccttacta aaagctgcct ttgtagagga
     3841 gtccatatat gtgggcgacg aacagctgaa tatgcttgca tcgctgaaga gcaagaacga
     3901 gctcattgcc gatgttattg ccctgctgca atcacccgca aagaatgtta tcgcttctct
     3961 tcaatcagga ggaaatattc tatctggtgt tgttaaaaca ctttctgaaa aggaaaaata
     4021 acttaaagtt tgaaatcaat taaacactta tcaaaatggc agatctcaag aaatttgcag
     4081 aagatttagt taacctttcg gttaaggaag ttaacgagtt agctaagatt cttaaagacg
     4141 aatatggtat tgaacccgct gctgctgctg tagctgttgc tgctgcacct gctgctggtg
     4201 ctggcgccgc tgcagccgag aaaactcagt ttgacgttat tttgaaagct gccggcggag
     4261 caaagcttca ggtggttaag ctggtgaaag atcttaccgg actggggctc aaggaggcta
     4321 aagatctcgt tgatgccgca ccaaagccgg ttaaggaagg tgtttcaaaa gaagaagccg
     4381 aatctcttaa agcacaactc gaagacgcag gagcagaagt tgaacttaaa tagcaaaagc
     4441 ctatattggg taaaggttta gaatctatcc attagattct aagccttttt attgttatat
     4501 ttttacagtt caacaaacac taatccaatg tctaataata acaataacaa ccgaattagc
     4561 ttttccagaa cgaagagtca atacgaatac cccgattttc tggaagttca gctaaagtca
     4621 ttcaccgaat tttttcagct gggcactagc cccgaagaac gcagacacga agggctgtat
     4681 aaggtatttc tggaaaattt tccgattacc gatacacgaa acaacttcgt tctggaattt
     4741 ctagactact ttgttgatcc accccgctac tccattgagg aatgccttga tcgagggctt
     4801 actttttgcg ttcccctgaa ggccaagctc aagctctact gcaccgatcc cgagcacgag
     4861 gacttcgata ccgttgttca ggatgtgtac ctcggaacca taccctacat gacgccccgc
     4921 ggaaccttcg ttataaacgg tgccgagcgc gttgttgtgt cgcagctcca tcgttcaccg
     4981 ggtgtctttt ttggacaaag cattcacccc aacgggacaa agctctactc agcccgaatt
     5041 attcccttta agggctcgtg gattgagttc gccactgata ttaataacgt gatgtacgcc
     5101 tacatagacc ggaagaaaaa gctgcctgtt actacgctgc tccgtgctat cggctacgaa
     5161 agcgataagg atattctgga aattttcaac ctggccgagg aggttaaggt taacaagatt
     5221 aacctgaaaa aaattgtggg tcagaggctt gctgcacgtg tgctgaaatc gtgggttgag
     5281 gattttgttg acgaggacac cggggaagtt gtatctatcg aacgaaacga ggtaatactc
     5341 gaccgtgaaa cagtggtcga gaatgaacat attgacacca ttattgagtc gggagctaag
     5401 tccatacttg ttcataagga aaatcaaact ttttcggact acgctattat atacaatacc
     5461 ctgcaaaagg atccctgcaa ctccgagaaa gaagctgtag tgtatatcta taggcagctg
     5521 cgtaactctg agcctcctga tgaggctacc gcacgtgatg ttatagacaa gctgttcttt
     5581 tcggataaac gatatgattt gggcgaggtt ggacgttacc gtattaacaa aaagctcaac
     5641 cttacaacct cccccgatgt aaaggtgctt acgagagaag atatcattga gattatcaag
     5701 tacctgattg aacttgtcaa ctccaaagca gaggttgacg acattgacca tttgagcaac
     5761 cgacgggtta gaaccgtggg cgaacagctg gctgcccagt tcagcgttgg gctggcaaga
     5821 atggccagaa ctatccgtga gcgaatgaac gttagggaca acgaggtgtt cacgcctatt
     5881 gacctgataa actctaaaac gctttcatcg gttattaact cctttttcgg cacaaaccag
     5941 ctgtcgcaat tcatggatca gactaacccg ctggccgaaa tgacccataa gcgtcgttta
     6001 tctgcacttg gacctggtgg actttccaga gaacgtgccg gttttgaggt tagagacgtg
     6061 cactacaccc actacggcag gttgtgcccc atcgaaacgc ctgaaggccc taatatcggg
     6121 cttatctctt ctctttgtgt ttacgcccga ataaacgatt tgggattcat tgaaactccc
     6181 taccggaagg ttgacggcgg aacggtgggc atgaacgatg acgatgtagt gtacctgact
     6241 gccgaggagg aggaggccaa aattattgcc caggcaaatg ctcctattgg tgatgatggt
     6301 tcgtttgttg aatccaaggt aaaagctcgt tacgaagggg atttccccct ggctgaaagc
     6361 gaatcaatcg accttatgga tgttgcacct aaccaaattg cctccattgc cgcctcgctc
     6421 attccttttt tggaaaatga tgacgccaac cgtgccctca tgggttcgaa catgatgcgt
     6481 caggcagttc cacttctaaa gcccgaaact ccgattgttg gaaccggatt ggaaggtaaa
     6541 gtggtgagcg atagtcgtgt gcaagttgtt gccgaacagg atggcgtggt ggagtatgtt
     6601 gacgccaacg agattgttgt aagatataac cgctcggaag atgaagtttt tgttagtttc
     6661 gaccccgagc ttaaacgata tagcttacca aaattcaaga aaactaacca aaatacatct
     6721 attaccctaa cacccattgt taccaagggc caaataatta aaaaaggcga tatccttacc
     6781 gaaggctatg ctactcgcaa cggagagctc gcccttgggc gaaaccttaa ggtggccttt
     6841 atgccttgga agggttacaa ctttgaggat gccatcgtaa ttaacgaacg gttgattagg
     6901 gatgatatgt ttacctccct tcatattgat gagtacatcc ttgaggttag ggatactaaa
     6961 cgtggaatgg aggagcttac agccgatata ccaaacgtat cggaagaggc tactaagaac
     7021 ctcgatgaga acgggatgat ccgtataggc gccaacgtgg tgcctggcga tatccttatc
     7081 gggaagatca cccctaaagg ggagtctgat cccactcccg aggaaaaact gctacgcgca
     7141 attttcggag ataaggctgg cgatgtgaag gatgcctcac taaaagcttc cccatcgctt
     7201 tacggcgtgg tagttgacaa aatgcttttt tcaagggcta ttaaggatga aaaggaccag
     7261 aaaaaatcca agtcaactga gaaaagtaag ctggaaaaaa tcgacaacga gtttaaccgt
     7321 caggttgccg agcttcaaac caagctggtt gataagctgt ttgttcttgt aaacgggaaa
     7381 acctcgcagg gcgttgtgga taatttcaac caggagctca ttcctcgtgg ggtgaaattt
     7441 acacataaaa tgcttaccga gattgactac atgaatgtta acccaaacaa atggacaacc
     7501 gataaggcta agaatgagct gattaaaact gttattcaga attacattct gaagtggaag
     7561 gagattgatg cggagtttaa gcgcaagaag tttaatgtaa ccatcggaga tgagctgcct
     7621 gcaggaatta tgcagctagc caaagtattt attgccaaaa aacgcaagtt aaaagtgggc
     7681 gataagatgg ctggccgaca tggaaataag ggaattgtgg ccaaggtggt tcgtgatgag
     7741 gatatgccgt tcctggagga tgggtcgcct gtcgatatag ttttgaaccc tctaggcgtt
     7801 ccctcccgaa tgaacctggg tcagatttac gaaaccgttc taggttgggc agggcaaaag
     7861 ctgggcgtta gctttgccac accgattttc gatggtgcaa ctcttgacga tataaccgtg
     7921 tacaccgaga aggccggctt accccgcttt ggtaaaacat acctgtacga tgggggtacc
     7981 ggcgagcgct ttgatcaacc tgcaactgta ggtgtaatct acatgatgaa gctcggacac
     8041 atggttgacg ataagatgca tgcccgttct ataggccctt actcccttat cacccagcaa
     8101 ccacttggtg gcaaggctca gtttggcggt caaaggtttg gtgaaatgga ggtatgggcg
     8161 ctggaagctt ttggggcttc ccacatcctt caggaaatac tcaccatcaa gtccgatgat
     8221 gtggtcggta gagctaaagc ttacgagtca attgttaagg gagaaccaat gcctgaaccc
     8281 ggcatacccg agtcgctcaa cgtgttgcta catgaactta gggggttggg cttaagtatt
     8341 aatcttgact agcccgattg cacagttctg actaaagact ttttttgctg tccagctgca
     8401 taacataact tttaactagt tgaatatgtc gttcagaaaa gataataagg ctaagagcaa
     8461 cttctcaaaa atcactatca gccttgcctc acccgaagaa atactggagc ggtcgagcgg
     8521 agaagtgcta aaacctgaaa cgataaatta cagaacctat aagcctgagc gagacgggtt
     8581 gttttgtgaa agaattttcg gtcccgtgaa ggattacgag tgccactgcg gaaagtacaa
     8641 acgcattcgt tacaagggta tcgtatgcga ccgttgcggt gttgaggtta ctgagaagaa
     8701 ggtacgcagg gagaggatgg ggcacatcac acttgtggtt ccagtggcac atatctggta
     8761 ttttaagtcg ttgcccaaca aaataggcta cctgcttgga ttaccctcca aaaaactgga
     8821 gtcaataatc tactacgagc gttacgtggt ggttaatcct ggcattaaag ctgctgatgg
     8881 agtcaaatac ctcgatttcc ttaccgaaga ggagtatctc gaaattattg attcccttcc
     8941 aaaggataac cagcatcttg acgataacga tcctaacaag ttcattgccg gaatgggagc
     9001 agaagcgctt aatttgctct tgtcccgcct aaacctggat gagctgtcat acgaccttcg
     9061 caacagggca aacaccgaaa catcccaaca gcgtaaaagt gaagcgctga aacgccttca
     9121 ggtggtagag gcctttcggg aatcgggcac cataaacaag ccggaatgga tgatattgaa
     9181 ggtaattcct gttattcctc ctgagcttcg tcctttagtg ccgcttgatg gaggaaggtt
     9241 tgccacttcc gacctgaatg acctataccg tagggtgatt attcgtaaca accgtctgaa
     9301 acgtcttatt gaaattaaag cccccgaggt gatacttcgg aatgaaaagc gtatgcttca
     9361 ggaggcagtg gactcgcttt tcgacaactc ccgcaagtca aacgctgtta aaaccgatgc
     9421 caaccgtccg ttgaaatcac tgagcgatag cttaaaggga aagcaaggtc gtttccgtca
     9481 gaacttgcta ggtaaacgag ttgactactc agcccgctca gtaattgttg ttggtcccga
     9541 gctgaagatg cacgagtgcg gtctcccaaa ggaaatggct gcagaactct acaaaccatt
     9601 tattatccgc aagctgattg aaaggggcat tgttaaaacg gtgaagtcgg ccaagaagat
     9661 tgttgaccgg aaggatcctg ttgtttggga cattctggaa aacgttttaa aggggcatcc
     9721 cgtgctactt aaccgtgctc caacccttca ccgcttgggt attcaagcat tccaacccaa
     9781 gctgattgaa ggtaaggcaa ttcagcttca cccccttgcc tgcacagcat ttaacgccga
     9841 ctttgatggc gaccagatgg cagtccactt gcccttgagc aacgaggcca tcctggaagc
     9901 acaaatttta atgctcggct cacataacat tctcaaccct gccaacggag cgcctattac
     9961 cgttccatcg caggatatgg ttcttggtct ttactacatc accaaaccta gaaaaggcgt
    10021 taagggcgag ggcttaattt tctactcgcc cgaggaggta ataattgcct acaacgagca
    10081 tgctgttgac ctacatgcca tcattaaggt tcggcttatg ttacccgata agaatgggca
    10141 gctgaccccg cagcttgttg aaaccactgt tggacgggtt ttattcaacc agaatgttcc
    10201 tgcagaaatg ggctacatca acgaggtgct aaccaagaag tcgcttcgcg atataattgg
    10261 tgctgttttg aagaaagccg gcacggctaa ggctgccaag ttcctcgacg acataaagga
    10321 tatgggatac tacatggcct ttaagggtgg actttcattc aacctcgacg atgtggttat
    10381 acccccggaa aaagcccagc ttattgagga gggctatgcc caggttgatg acgttatggc
    10441 caactacaac atgggtctga ttaccaataa cgagcggtac aaccaaatta ttgatatctg
    10501 gacacatact aacgccaagc ttacccttac cctgctaaaa accctttcgg aggacaacca
    10561 gggctttaac tccatctaca tgatgcttga ttctggagcc aggggttcga aggagcagat
    10621 acgccagctt ggaggtatgc gtggcctgat ggccaagcct caaaagtcgg gatcggtggg
    10681 aaacgaaatc atcgagaacc ccattctatc gaactttaaa gaggggctat ccgtgttgga
    10741 gtacttcatc tctactcacg gtgcacgtaa gggtttggcc gataccgctc tgaaaacagc
    10801 cgatgccggt taccttaccc gaaggttggt tgacgtatcg caggatgtta ttattaccga
    10861 gaacgattgt ggaacgttac gtggtttggt agctactgct atcaagaaga atgaggatgt
    10921 ggtggaaacc ctttacgaga gaattcttgg aagaactacc gttcatgatg tattccaccc
    10981 gttaaccggt gagcttattg tggctgcagg cgaggaaata accgaagaaa ttgcctccat
    11041 tattgaagat tcaccaattg aacaggtcga aattcgatcg gtactcacat gcgagtcgcg
    11101 aaagggtgta tgctccaagt gctacggtcg taacttggcc accggtcgca tggtgcaaat
    11161 tggagaagct gttggcgtta ttgccgccca gagtattgga gaacccggta cccagcttac
    11221 acttcgtaca ttccacgttg ggggaacggc ttccaatata atttccgatt ctaaaattat
    11281 tgcccgcttt gatggcagag ccgaaattga agagctcaaa actattacca cagaggatga
    11341 actcggccaa ccgtacagca ttgtaattgg ccgtctggct gaaatgcgca tagttgacct
    11401 gaataccggt ataacccttt ctacccacaa cattccctac ggatcgaaac tttacatttc
    11461 aaacggcgat accataaaga agggtcagct tatctgcgag tgggatccat ggaacgctgt
    11521 gattgtttca gaggtttcgg gtagggttga attcgacgcc cttatcaagg aggttacctt
    11581 cagggaggaa tccgatgagc aaaccggata ccgcgaaaag gttattatcg aaacccgcga
    11641 taagagcaaa aaccccgccg ttaaaattac cgacgaaaag ggcgaaggca ttaggatgta
    11701 caacctgcca gtttctgccc acgttgttgt tgacgagggt acagttataa gagcgggtga
    11761 tattgttgct aagattcctc gcgcaatggg gaaatcaggc gatattaccg gaggtttgcc
    11821 aagggtaacc gagctctttg aagcacgaaa tccttctaac cctgcaattg tttcagaaat
    11881 tgacggtgaa gttacctttg gtaaggtgaa aagaggtaac cgtgaaatta tggttacctc
    11941 caaaaccgga gaggttaaga aatacttggt tccactctcc aagcaaatac ttgttcagga
    12001 aaacgactat gtgaaagctg gaatgcctct ttccgacgga gctattactc caactgatat
    12061 tcttgccata aaaggtccaa cgcaggttca ggagtatatt gttaatgagg ttcaggaggt
    12121 ttacaggctt cagggggtta aaatcaacga caagcacttc gaggttattg tacgccagat
    12181 gatgcgcaag gttgaaattg acgaccctgg cgatactcga ttccttgaaa aacaggtggt
    12241 tgataagtgg gagtttcttg aggagaacga ctatatcttc gataagaagg tggttatcga
    12301 tcccggagac gctcctaact atagacctgg acaaattatt actgcccgga tgcttaggga
    12361 tgaaaactct gccctgaaac gtaaagacct aaagcccatt caggctaggg atgctatccc
    12421 tgcaacttca agccaggtgc tgcaaggaat cacacgtgct gcactgcaaa ccaacagctt
    12481 catgtctgcc gcttcgttcc aggaaaccac taaggttttg aatgaggctg ctgtaagggg
    12541 taaggttgat aaccttgaag ggctaaagga gaatgtgatc tgtggacacc ttatccctgc
    12601 cggcactggg cttagagagt ttgacagaat aatagtttac tcaaaggatg agcatgagtt
    12661 tgttgaaaaa gcaggtaagt cggtttcgct tacgagcaac gactagccat agcaagaaaa
    12721 tatccttaaa agggttggct ttactgccaa cctttttttt gtccttcctc cagcagtata
    12781 atctaagcta aaagttgctg gctgctttca taatattgaa gcacccatat atgggcaagc
    12841 gttaccttca atttcggcga agcccaaata tggttgctcc atcagctcca cccccatact
    12901 tatgagatat gagtcaatgt acttgttatc agaaacctaa ataaattatg cagggattga
    12961 atgctgcata acggaaacga gtcaacgttg gtttaaaaat tttaccaaag taaaggccac
    13021 tatcaccgat gctaatcttt gccgaatttg aaaaaattac taactttccg ttctaaaaat
    13081 taataataat acgccatgcc tgaaaaaatg aatcctatca atattgagat taacgatgaa
    13141 attgcccagg gtatttactc aaacctagcg gtgataaccc attcatcttc agaattcatt
    13201 attgactttg tgcgggttat gcccggtatc cctaaggctc aagttaagtc aagaattgtg
    13261 ctaactcccg atcatgccaa gaggttaatg ctggccctgc aggaaaacat tggtaagttt
    13321 gaagctgcaa atggaaccat taagctacct gaggggagtg agggtatgcc accattccca
    13381 ataaatttcg gaggtaaggg gggcgttgcc tagtgtgtgt tggcattggc attctttgtg
    13441 catatattac tgaaatacgg tagtttagat tgatgtttta atttcaggag tttggttcac
    13501 ctgaataaat gttgtagcgg gatactattt tctaaacagg tattcacgtt tactttcaaa
    13561 cttatacata tttgttgggc aagataaaag taaaatttac tatgaagaag tttgcagatt
    13621 ataccctaat ggcattgctg gccggtatgc tggcatgtag caacaacaac gagcaactgg
    13681 ttacttcccc atcgggagaa gtgaaggtaa ttttcagcct cactgccgat aaacagccct
    13741 cttacagggt ttactttaaa gactctcctg tgattgagac ctcaacactt ggattcgatt
    13801 tcaaggatgc cccatcgctg tccaagggga tgagagttga tagcgtgttg agtaatgagt
    13861 ttaacgaaac gtgggagctg ccgtggggcg agcaacggct tgtgaagaat cattacaacg
    13921 agatgaaagt tttcctgtcg gagaaaaaat caccaaaccg ccactttgcg gttacatata
    13981 gggtttacga cgatggaata ggatttcgtt acgaatttcc ccaacaggaa agtatgggcg
    14041 atgtaacaat caccgatgaa aatacacagt ttaagctaac cggcgaccat ttgtgctggt
    14101 gggagcccgg cgactgggat atatatgagc atctatataa cacaacttcg tttagcaaga
    14161 ttgacgccac caaaaagcga aaccatcgcg atttggcaca aacctacatt ccggaaaacg
    14221 ccgtaaatac tcctgtaacc atgaaaacta gccaggggct ataccttagc tttcacgaag
    14281 ccgctgttaa taactatgcc ggagtaacct taaaagttga tacggttaac tactccatga
    14341 caactgagct ggttggcaac gattctggag ttaaggtggt taccaaaaca ccctttaaca
    14401 ctccctggcg tatgattcag cttgccgaaa aacctggtga tttgatagaa tcaaggctga
    14461 tacttaacct gaacgagccc aacaagctgg gcgatgtttc atggtttaag cccacgaagt
    14521 acgttggtat atggtgggag atgcacctgg gaaaatcaag ctgggatatg gccagtggga
    14581 agcatggggc aactactgca aatgctaaac gatatataga ctttaccgct aagaataata
    14641 tcggagcctt acttgtagag ggttggaata ccggttggga gcactggatt ggatttgatg
    14701 atagggaggg agtttttgac ttcgtaaccc cctatccgga ctacaacctc aaggaggtgg
    14761 ttgagtatgg ccgcaagaag ggtgttgatc tgattatgca ccacgaaaca tcatcggcgg
    14821 ttaccaccta cgataagcag ctcgataccg cctttgccct aatcagcagc ctgaacctcc
    14881 acatggttaa aacaggatac gtagggaaga taatacccaa aggagagtac catcatgggc
    14941 agtggatggt taaccactac cgaagggttc ttgaagtggc cgcaaagcac aaggttgccg
    15001 ttgatgttca tgaaccgttt atggcaacag gcgagcgcag aacatatcca aacttcgttt
    15061 ctcaggaggg attccgggga caggagttca acgcatggtc gagcgatggg ggaaatcctc
    15121 ctgagcattt agctattatt cccttcacct gcctgctggc cggacctatg gactatacac
    15181 cgggaatctt caacaccaag ctgaaaccct acaaacccaa caaccaggta aataatacac
    15241 tggcacagca gcttgccctt tatgtagtca tctacagccc aattcaaatg gctgccgatt
    15301 tgcctgagaa ttatgaaaac catcctgcat tccagtttat ccgtgatgtg ggtgtaaact
    15361 gggagcaaac cagggttctt aacggtgaag ttggcgatta tgttaccatt gcccgtcagg
    15421 aacgcggaac aaaccgttgg tttattggtg cggtaaccga tgagaattca agggagctca
    15481 gcgttaagct ggattttctg gaaccgggta aaaagtattc agcaaccctt taccttgatg
    15541 ctcctgatgc ccattgggat aagaacccct cggcatacga gataaagaaa atggaggttg
    15601 acagcagcac agagctaaac ctgtatttag caccaggagg tggagcggca atttcgcttt
    15661 tcccgctata agtgagggtt gaaaataaaa tatcagaaga gtccccggcc agtaaccggg
    15721 gattttttta acggttgaag gtatggtgac gtgcgggatt acgaagcagt ttcctgtcca
    15781 ccgatacgaa agtttaagcg ggctacaacg cttgaatacc cactgaaacc cttatggcat
    15841 atacactttg t
//
LOCUS       DAOPHX010000050        15780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_4231_length_15780_cov_4.916502, whole genome shotgun sequence.
ACCESSION   DAOPHX010000050 DAOPHX010000000
VERSION     DAOPHX010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 15780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15780
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4231_length_15780_cov_4.916502"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PK502_05275"
     CDS             <1..752
                     /locus_tag="PK502_05275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPH00259.1"
                     /translation="SRQPGRRFTDKTKYMDNQETISKFYDDFSFKQVKTGKNLRHYTI
                     TKNLKKKGIGKSNKILEIGCGIGTLTDILLQIAPKAKITALDISPKNIEIARSRFKSK
                     RVIFMVADASDNFTLDEKFDFIVLADVIEHIPFERYHSLFSNLKRFSHDDTKIFINIP
                     HPNLIKHLKETKPESLQIIDQSVYPTILYQHLYANFFIIDEKKDYSIFYQTPDYSYYW
                     LSSLSEYINTNYNKTIPLPIRMLKKFLLRIF"
     gene            940..1806
                     /locus_tag="PK502_05280"
     CDS             940..1806
                     /locus_tag="PK502_05280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPH00260.1"
                     /translation="MFAGYWSVIPVLFGKIFRKKVFIILGGTDCVSYPTFNYGSLRKP
                     LLKRAIYVSIKHATCILPVHESLIEFTNSYYDNSKQGIKNHFKPIKTAIRTIYNGYDP
                     KVSQLTIESSNRILNSFITVALVNNKTRVILKGIDLILESAREFPEASFTIIGIDNKI
                     INKLDIPKNVKIFPPIPYDLLTEKYLQSEFVIQTSISEGFPNALSEAMSFGCIPLISP
                     VGAMPTITKDIGFTVNRRSPVELSLAIRNALNLTNEEKINLRMQAHTQIVNNFSGIAR
                     IGKLAQTIEEFN"
     gene            1812..3269
                     /locus_tag="PK502_05285"
     CDS             1812..3269
                     /locus_tag="PK502_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPH00261.1"
                     /translation="MPSKSFIKSSFIYTVVGSLPLATSILLLPFYGNSSLLSTADFGL
                     LAIYIILSELAKILFTFSADSFLGFNYIHYNNSKSNLQRFIGTTSVFMLVYGVLMLLL
                     FCVLGGSAIKLLYPGKNISFYPFGFLSVLTGLLNGIFKAYSTLMIYREKPAPFLWSNI
                     IHFTLVLAVSITGLYLYPQTLLGPIWGRFVGAIATFLWSLTYFVSASRFRVDFNILKK
                     LLSYSAPLYLFGILYWIVSNIDRYFILGLLSEKDVAIFDFAVKLTLAIEFLQNGLSSA
                     IYPKIFQIWKKNDDVPHGNVEINRFFHVFALITVVGIPVLYLSVPLLVPLLVNNSELY
                     ASFNLLPLLFAGMVSRIWYYYLVAPIYYFKQTKVMPLVFGFAAIFQIASTYILIQLNG
                     IDGAVWANLLTKLFQVFLLYLFVRRFYTLRVNVKKLIVFPAVYMMLLISAEIFLKPVN
                     IYLLNLIHIVIITVLAYSFFRNELKWENIRTALKK"
     gene            complement(3279..5549)
                     /locus_tag="PK502_05290"
     CDS             complement(3279..5549)
                     /locus_tag="PK502_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPH00262.1"
                     /translation="MERAYSKPFVVRITILGFIAGLAVILLAFFIAAVTGGYSFNFSG
                     IVEMHRIMPVLYFLDIFPLLIALASFYISRYISRIRMESESSSEETIHRQRKLYHFVE
                     KLQEGDESVEYIPDEGDALGKAIVNLRNNISQSRKEEEIRRKEDEQRRWSAEGMARFG
                     EILRLNNDNVEELSFQIIRNLVNYVGANQAAFYLLEDDDEADVHFRMTACYAYERRKY
                     ADRIVPWGDGLVGACALEKETIFLKKVPDSYLNITSGLGKANPRCLVLIPLKLNDQVH
                     GVLEVASFNIFEKHQLEFMEKIAESVATTISTVKINLRTAKLLRESREQAEELALKEE
                     QMRQNMEELQATQEEAARQSEKFISFSNSVNHTMIRADFDVKGTLTYANTKFMQKLEY
                     SSTAEVEGKPLDIFLDRRDRAWFEEVWKKLSKGGKHFEGNMKLATKTGKELWILATFT
                     CMRNTMGDVDKILFLALDASGQRAKNLELEGQVNAFSRSVVKADISPTGDMIAVNDMY
                     IETLGLPYEVAMKKNIFETVSPVDRQNVEDAWDDIIHGNPFDGKLRVNTGNNDEKWLQ
                     VSLVPVNDTNGEIAKVLLIAYDITSEVHLAIEVQQKEETIRKQEELIQQKEVEQNKKL
                     RETREEITNQYRETEKIKVRNEKLLEELSEAIVTTDHDGLILFMNKAAENLFTVKHDE
                     VANQNVRILFSDTVKDNFVTTYLDPARKKIVGQRQMVTLNPRKGKEVNATMVVYEVKT
                     GREITYTAFFQPTTNS"
     gene            6262..6648
                     /locus_tag="PK502_05295"
     CDS             6262..6648
                     /locus_tag="PK502_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPH00263.1"
                     /translation="MSRILIVDDIHVNRLLLKEILTKISVDCHEAKDGKEALEILQNE
                     KVDVVLMDIEMPVMNGVETTRYIRQNFPFPLKSIPIIALTAHNPADFFEDFHNTGFNY
                     LLTKPYSIERIQKAIDETLKQEKQQS"
     gene            6898..8118
                     /locus_tag="PK502_05300"
     CDS             6898..8118
                     /locus_tag="PK502_05300"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPH00264.1"
                     /translation="MTSRDLAQLIAAHSRLILPNLGAFLHKDAADETFSASKVTFSPF
                     LKYNDGKLEDYLSKTTGISKEESFKQVHALVSSINQQLESSGKYPIPGLGQLVRDNQG
                     AISFVADTSVNTDTIEANFSGKISNSPPKDDGKTGEESMITGDNKASSYNSTPANRAS
                     SIKINVEADTSNPDSTAKSAIEDSINKTDSEPEKQTQDKAENIGKLENEPVKGYFKMG
                     KRSSGKKKSKLILPALFVITSLVVIFIIALVIRHIALSPSYNYSDEDVTPENNYTPGI
                     SNMTTPKPKDKIDRAYDSLVGQIKKEESNPSSPEPSIEDKIEQEVIENYKAKDGEPTG
                     KYYLIVGSYKNADNARNMASKLKDKGFKSSVVSRPDGISCVALGAYSSNGEAEKAKLA
                     YEKQFPGIWILTVQ"
     gene            8189..8923
                     /locus_tag="PK502_05305"
     CDS             8189..8923
                     /locus_tag="PK502_05305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HPH00265.1"
                     /translation="MTSKSKPLSNSKKLCTYPLIGEVSYIRKSGLKRVSLRVNAYGDV
                     RVSMPPFISFKYAEEFMLSNGEWIIEKKGRSFLGKNLRDNDSIHTRYHTITLFQDSQS
                     TGIRVIQSNNQARIHYPLTMAKNDPAVREVLKKVVEHTYMKEAKDDLPLRTATLAAAH
                     GFSFRRVSISRATTRWGSCSPENNIRLSLHLMRLPDHLVDYVIIHELCHTVEKNHGIN
                     FWKLMNEHCNGHARQLAREIKEYHAY"
     gene            complement(8961..9242)
                     /locus_tag="PK502_05310"
     CDS             complement(8961..9242)
                     /locus_tag="PK502_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00266.1"
                     /translation="MSKIRDFKKEVKHMVKLFLNECYTQLSFSPEFNQENILDIISDV
                     IVLQRDTVSKLSRKNFAKGSRKNIDYQKVASEFYTQIVELTERLNSLDY"
     gene            complement(9428..9892)
                     /locus_tag="PK502_05315"
     CDS             complement(9428..9892)
                     /locus_tag="PK502_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPH00267.1"
                     /translation="MKSRSNLTPPEVNDIINRSLVCHVGMVDTNGMPYVLPFNFGYQN
                     DRLYIHSGPEGKKIGIWKSNPNVCVEFSTDYQMHHQNDSVACSYSMRYKSVLIYGMVK
                     EIDDIDKKKEVLEIFMLKYTSLQNFKYSTPALANVKVFEILPEKIEARAYGY"
     gene            complement(10110..12968)
                     /locus_tag="PK502_05320"
     CDS             complement(10110..12968)
                     /locus_tag="PK502_05320"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HPH00268.1"
                     /translation="MVEIKYKGLSEEQVVESRAKFGENVLTPPVRKPWWRLLLEKFND
                     PIIRILTIAAVIAIAVGAVNGHYLEGVGIIVAIFLATFMSFINEYRAGKEFDILNQVN
                     EENPVKVIRNGGVTSIPKKDVVVGDIVIVDRGDEIPSDGVLLESVSLSVNESSLTGEP
                     IAQKSYDSSLDQGNNTYPINRVYKGTAVVEGHGIYETTSVGDSTEIGKTARESTLQVD
                     IQTPLTKQLNRLSDFIGSIAFLLAILIFVLLVVADVAKGTSGLSSNQQFILVSLVVAA
                     VVALMKFWLPSLNFLVKAFGAKRGIPDIFAKPKINSYLLSFLLGLAVVVVAIPIAFAK
                     GIDLADSANWFSVDFISKLLNYFMVSMTLIVVAVPEGLAMSVTLSLAYSMRKMTADNN
                     LVRKLQATETMGAVTVVCTDKTGTLTQNRMAVLESSFSAVDDISENSLNAELIKYSMA
                     VNSTAHLDFTEKSPAVIGNPTDAALLLWLNNQGINYQSIRNDFTIEQQLPFSSEKKYM
                     LTVGKAGSLNKRILLVKGAPEVVISMCEVDDILQAKLTGLLESYQQKSLRTIAFAYRF
                     LDHEVVNENEKPSNLNFIGVVGIADPVRLDAAEAVAQCHRAGVDVKMLTGDNIVTATS
                     IGKQVNILSGNAEANVISGPDFEALSEDEARRMANQLKIMYRARPSDKMRLVRLLQEN
                     GQVVAVTGDGTNDAPSLNRADVGLAMGSGTQVAKEASDIILLDDSFSSIVNAIRWGRT
                     LYGNIQKFLFFQLTINLLAVVIVLTGPFTGITLPLTVTQMLWVNLIMDTFAALALATE
                     QPHAGVLNKPPRSPKEFIVTPSIFKGILLASASFLIILLGMLIFFGRDGNVSSYELTL
                     FFNLFVMLQFWNLFNSRSLGDYSSALKGLFHNKGFITIAFVILLGQVVIIQWGGEIFR
                     TVPLSLSDWVKIFVASSLVLWIPEAYRFFKRISLKKVR"
     gene            13229..13945
                     /locus_tag="PK502_05325"
     CDS             13229..13945
                     /locus_tag="PK502_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPH00269.1"
                     /translation="MGRAFEYRKARKLKRWGNMAIVFTRLGKEITMVAKSGGADPDTN
                     PKLRLLIQNAKSENMPKDNIERAIRRATDKDTKEYKEVVYEGYGPYGVAILVETATDN
                     PTRTVANIRSYFNKHGGSLGTSGMLDFMFEHKCSFRIDGKDSISLDELELELIDYGVE
                     EVFVEDDSILIFADFAQFGPVQRYLEGKGLHILNFQFERIPTDTKKLTPEQQEEVEKL
                     VDKIEEDEDVTHVFHNMEIA"
     gene            complement(13995..15098)
                     /locus_tag="PK502_05330"
     CDS             complement(13995..15098)
                     /locus_tag="PK502_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPH00270.1"
                     /translation="MDEKLIVIAIESYAKAVVLQSYLEANGIECFLRNENILQGAVSE
                     GVKVQIRESDAEKALRLLSEQKFSEEAKKREKPLRKILVPVDFSEPSQNAARFAVMLA
                     EKYNAEIKLLHVFNSPVVDMIPFADVSGIQIDFDINFNLLYKAAKEKLIKFHADLVEF
                     ARQMGFNSVSIGYTLQEGYAAYGIVEVSKRYKPGIIVMGTKGEGFKSTELVGSVASEV
                     SEETKVPLLAIPEKAVLKGVDEVKNVLYATNLDESDFGSIRKLIRILSPFNFNLHCVN
                     MTNRPDNVEIKALMSSLKNYLYQVNPELSVEYSIVECKDMVKSFIDYIYDKKINLVAL
                     TSVKRSLIYKLFNPSLSKKMLYQSNVPVLLFQA"
     gene            complement(15201..>15780)
                     /locus_tag="PK502_05335"
     CDS             complement(15201..>15780)
                     /locus_tag="PK502_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00271.1"
                     /translation="IEDYEEITALKAKVELGSVTVLSLTYASTYFPTQSFDLTAEFGG
                     YKLSVSIDYSDSSSEIYSLSKLQISFKQGNTTLYALDLNLNVSMISETNTSVKLNATL
                     IMKSLKFTATADFNTDDLDYETNPNVDLNEYISVKLYTTSDREIGHFSFVRNFETGEL
                     TVKFIYADGTEVNAEELMPQISAFIQSLLEEE"
BASE COUNT         4871 a         3379 c         2973 g         4557 t
ORIGIN      
        1 cctcaaggca gccaggaagg aggtttacgg ataaaactaa gtatatggat aatcaagaaa
       61 ctatctcaaa gttttacgat gacttttcgt ttaaacaagt taaaaccggg aagaacttac
      121 gacattacac aataactaaa aaccttaaga aaaaaggcat tggtaaatcc aacaaaatcc
      181 ttgaaatagg ttgtggtatt gggacactaa ccgatatctt gctgcaaatt gcccctaaag
      241 ctaaaataac ggcactagat ataagcccaa agaatatcga aattgcaaga agccgattta
      301 aatctaaacg agttatattc atggttgccg acgcttcgga taattttacc cttgacgaaa
      361 aattcgattt catcgttctt gccgatgtaa ttgagcatat accctttgaa cgataccatt
      421 cgctgttttc gaatctaaaa cgatttagcc atgacgatac aaaaattttt attaacattc
      481 ctcaccctaa cctgattaag catttgaaag aaaccaaacc cgaaagttta cagattattg
      541 atcaatcggt ttacccaact atcctctatc aacatttata tgctaatttc tttattattg
      601 acgaaaaaaa ggattatagc atattttacc aaacacccga ttattcatat tactggctta
      661 gttctttatc agaatatata aatactaatt acaataaaac tattccacta cccattcgaa
      721 tgctaaagaa atttttgtta aggatttttt aaaatgaata tcctatacat ttcgtcatac
      781 aaccccagtt ttgttcacaa ggatataaca ctcctttccg aaaggttcaa ggtaacctca
      841 ttgctttacc cgtggaatgc gataaatact ccaattaatc ttttaagaca actaatctac
      901 ttaatttatc gaattaataa aattgataag ataatcataa tgtttgccgg ctattggtca
      961 gtaattccag ttctgtttgg aaaaattttt cgcaaaaaag tatttataat actaggtgga
     1021 accgactgtg tatcttatcc aacttttaat tacgggagct taaggaaacc gcttttaaaa
     1081 agggctattt acgtatcgat aaaacatgca acatgtatac tgcctgttca tgaatcgttg
     1141 attgaattta ctaattcata ttatgataat agtaaacaag gaataaaaaa tcactttaaa
     1201 ccaattaaaa ctgcaattag aactatttat aatggttacg atccaaaggt ttcgcagcta
     1261 accattgaat catcaaatcg tattttaaat agttttataa cagtggcatt ggttaacaat
     1321 aaaacacgag taatactaaa aggtatcgat ttgatacttg aaagtgcccg tgaattccct
     1381 gaagcaagtt tcactattat tggcatcgac aataaaataa taaataaact tgacatacca
     1441 aaaaatgtta aaatatttcc ccctatacca tatgacttac ttaccgaaaa atatctccaa
     1501 tcggaatttg tcattcaaac atccatttcc gaaggttttc ccaatgcctt aagcgaagcc
     1561 atgtcgtttg gctgtatccc tttaatttcg ccagtaggcg ctatgcctac aattacaaaa
     1621 gatattggtt ttactgtgaa tagaaggagc cctgttgaac tttctcttgc cattagaaat
     1681 gccttaaact taacaaatga agaaaaaata aatttacgta tgcaagcaca tacccaaatt
     1741 gtaaataatt ttagcggaat tgctcgaatt ggtaaattgg cacaaacaat tgaagaattt
     1801 aattagattt gatgccctct aaatcattca tcaaatcttc gttcatctac acagtggtag
     1861 gctcgcttcc actggctaca agcattctgc tgcttccatt ctacggaaac tcctcactac
     1921 tttcaacagc tgacttcggg ttgcttgcca tatacatcat tcttagcgag ctggcaaaga
     1981 ttctttttac tttttccgcc gatagctttc tgggattcaa ctacatacac tacaacaata
     2041 gcaagagcaa cctgcaacgt tttattggaa ccacatccgt attcatgctg gtttacggcg
     2101 tattaatgct attgctattt tgtgttttgg gcggctcagc cattaagctg ctctacccgg
     2161 gcaaaaacat aagtttttat ccctttggat ttctttcagt gttgacgggt cttctcaacg
     2221 gcatttttaa ggcatactca accctaatga tatatcgtga gaaacctgca ccttttttat
     2281 ggtccaacat tattcacttc acccttgtgc tggcggtttc cattaccggc ctgtaccttt
     2341 atccccaaac cctcctggga cctatctggg gacgcttcgt aggggcaatt gcaacatttc
     2401 tttggtcgct aacatacttt gtatcggcca gccgatttag ggttgatttt aatattctta
     2461 aaaaactact ttcctacagc gcaccgctct acctttttgg aattctttac tggattgtgt
     2521 caaacattga ccggtacttc atcctaggcc tactttcgga aaaagatgtg gctattttcg
     2581 actttgcagt taagctaacg ctggcaatag agtttttaca aaatgggcta tcgtcggcaa
     2641 tatacccgaa gatttttcag atatggaaaa agaatgacga cgttccgcat ggaaacgtgg
     2701 agatcaaccg attcttccat gtttttgccc taattacggt tgttgggatt ccagtactct
     2761 acctctcggt tcccctgctg gttccattgc tggttaacaa cagcgaactt tacgcctcat
     2821 tcaacctcct acccctcctt tttgccggca tggtgagccg tatctggtac tactaccttg
     2881 tggcgccaat ttactacttt aagcaaacca aggtaatgcc actggttttt gggtttgccg
     2941 caatttttca aatagccagc acctacattc ttatccagct taacggtatt gacggtgcag
     3001 tatgggcaaa cctgctcaca aaactttttc aggtattcct tttatatctt tttgtaagga
     3061 gattctacac cctgagggta aatgttaaaa agctaattgt tttcccggca gtttacatga
     3121 tgctactcat ttcggcagaa atatttttaa agccggtaaa tatttacctg ctcaacctca
     3181 ttcacattgt aataattacg gtgttagcct attccttttt ccgtaatgag cttaaatggg
     3241 aaaacatccg gacagctttg aaaaaatagc ccaactctct aggagttggt tgtaggttga
     3301 aagaatgctg tgtaggtaat ttccctaccg gtttttactt cgtaaacaac cattgttgcg
     3361 ttaacctctt taccctttcg agggtttagt gttaccatct ggcgttgccc aactattttt
     3421 ttccttgcag ggtctaaata tgtcgtaaca aagttgtcct ttacagtatc ggaaaacaga
     3481 attcgaacat tctggtttgc tacctcatcg tgtttaacag taaataaatt ctcagcagcc
     3541 ttattcataa acagaattag cccatcgtgg tcggtggtta caatagcttc ggaaagctct
     3601 tccagaagtt tttcgttacg aactttaatt ttctccgttt ccctgtattg attagtaatt
     3661 tcttcacgcg tttcgcgtaa tttcttattt tgctctactt ccttttgttg gattaactct
     3721 tcctgttttc taatggtttc ctctttctgc tgaacttcaa tggcaaggtg cacttcactg
     3781 gtaatgtcat aagctatcag taaaaccttg gcaatttcac catttgtatc gttaaccggg
     3841 acgagggata cctgtagcca cttctcatca ttatttcccg tatttaccct tagctttcca
     3901 tcaaatggat taccatgaat aatatcatcc catgcatcct caacattttg gcggtcaacc
     3961 ggtgaaacgg tttcaaatat gttttttttc atggccacct cgtaaggtag tcccagcgtt
     4021 tcgatgtaca tatcgtttac tgctatcata tctccggttg ggctgatatc ggctttaact
     4081 acagatcggc taaaggcgtt aacctgacct tccagctcaa ggtttttcgc tctttggcca
     4141 gaagcatcaa gagctaaaaa gaggatttta tcaacatcac ccattgtatt tctcatgcag
     4201 gtaaatgtgg ccagaatcca tagctcctta ccggttttgg tagctagctt catgttaccc
     4261 tcaaagtgtt taccaccttt gctaagcttt ttccacacct cttcaaacca tgccctatcc
     4321 cgtctatcta aaaaaatatc caaaggtttt ccttcaactt cggcagttga ggagtattcc
     4381 agcttctgca tgaatttggt gttggcgtag gttagggtcc ccttaacgtc gaaatcggcc
     4441 cggatcatgg tgtggttaac cgagttggag aagctgatga acttttccga ttggcgggca
     4501 gcttcctcct gggtggcttg caactcctcc atgttctgac gcatttgctc ctcctttagg
     4561 gcaagttctt cggcctgttc gcgtgattcc ctcagcaact tggccgttcg taggtttatc
     4621 tttaccgtgg aaatggtagt tgccacgctt tcggcaatct tttccataaa ctccagctgg
     4681 tgcttctcga atatgttgaa cgaagcaacc tccagcacgc catgaacctg atcgtttagc
     4741 ttaaggggta tgagaaccag acaacgtggg tttgccttac ctagtccgga tgtaatgttc
     4801 aggtagctgt ccggtacctt cttcaaaaaa atagtttcct tttcgagggc acatgcacca
     4861 accaaaccat cgccccaggg aacaattctg tcagcatact ttcgacgctc gtaggcgtag
     4921 caggcagtca tacgaaaatg aacatcagcc tcatcgtcat cctcaagaag gtagaatgct
     4981 gcctggtttg caccaacata gtttaccagg ttcctgatta tttgaaatga tagctcctca
     5041 acgttatcat tatttaacct gagtatttcg ccgaaccggg ccattccctc agcgctccat
     5101 cggcgctgct catcctcttt tcgccttatc tcctcctcct tacggctttg gctaatgttg
     5161 ttgcgaagat ttactattgc cttgcccagc gcatcaccct catccgggat atactcaacg
     5221 ctttcgtctc cctcctgaag tttttcaaca aaatggtata gcttccgctg cctatgaatc
     5281 gtttcttccg agctactttc ggactccatc ctgatacgtg agatgtagcg tgaaatgtag
     5341 aatgatgcca gagctataag caatgggaaa atatccaaaa aatataaaac cggcataatg
     5401 cgatgcattt caacaatgcc gctgaagttg aaagaatacc ctccagtaac cgctgcaata
     5461 aaaaatgcaa gcagaataac agctaaaccg gcaataaatc ctaaaatggt tattctgact
     5521 acaaagggtt ttgaatatgc tcgctccatg gcagtattat ttttgatttt aaaagtaggt
     5581 attttttcaa ttgaatggaa aaattcgatt ccaaatttag cgtgttacca gccaaaatcg
     5641 aggccctatc cgatacctat tcttcccaaa gtcagcccag ctataccgga taagtgcaaa
     5701 tgaacaaaat tcatagcaaa caaactaaaa tcaacagaac gtattaccag aactacctga
     5761 tattttgaag tatcaacaaa attgaaaaaa ataagcgcaa tacccgtaag cacagaatca
     5821 actttaatta ttgggtgtgc ctaaaaagta gtatttcttt aaccaaccat attcaggagg
     5881 gaagccattg gtttttccac agctcatacc atcgcacctg aaatttgcac tcaaaatcct
     5941 gaaatctgcc tacgaaacag cagcatttta gcctaaaata atactacaac acatataggt
     6001 actgccacaa ttagacctaa agcatccatg accagcagtc catttaagct accaaaacgc
     6061 caaatatgtg gggcttaaaa aaattgtttt tctctcactc tccgaaaggt catggcttgc
     6121 ttccgcacca taagatgagc acgcttataa ttcttccggt gattttttaa aaaaaagtta
     6181 agctaaatag ggaaaaatta cgtatcatac agcattacca accactaaaa cattaaattg
     6241 tattaagcaa aacatacaaa catgagcaga attttaattg ttgacgacat ccacgttaat
     6301 cggctactac taaaggaaat actcacaaaa atcagcgttg actgccatga ggctaaagat
     6361 ggaaaagaag ccctggaaat tcttcaaaat gaaaaagttg atgtggttct tatggatatt
     6421 gaaatgccgg taatgaatgg cgtggagaca acccggtaca ttcgtcagaa ctttccgttc
     6481 cccttaaaaa gcattcccat aattgcccta acagcccata acccggctga tttctttgaa
     6541 gattttcata acaccggttt taactacctg ttaacaaaac cctactccat tgaaaggata
     6601 caaaaggcta tagatgaaac tctaaagcag gaaaaacagc aatcctgaat tttcaagttc
     6661 caatattaaa gcaaggcatt ccttaacgtt taattattca gtaaggcaag cccttttccc
     6721 agcaaatata ggtaaaattg aacaccaccc cggtttgcga ataatatgga taaaccaact
     6781 tattatagcc tattcgcagg taataacctt catttttttt aaagagctcc ttttactcct
     6841 tactgtaaaa tatttttaac tttacagcgc tatttcaatc aagggaaaaa ggtaaaaatg
     6901 accagcaggg atttagctca gcttattgcc gctcactcca ggctaattct tccaaactta
     6961 ggagcattcc tccacaagga tgcagctgat gaaacgttta gcgccagtaa agttaccttt
     7021 agcccatttc taaagtataa tgatggaaag ctggaggatt accttagtaa aacaacaggc
     7081 ataagcaagg aggagtcgtt caaacaggtt catgcgctgg taagtagcat taaccagcaa
     7141 cttgagtcgt ctgggaaata ccccataccc ggtttaggac aacttgtccg cgataaccaa
     7201 ggagctattt cctttgttgc agatacatca gtaaacaccg acaccataga agctaatttt
     7261 tctggtaaaa tttccaattc ccccccgaaa gatgatggca aaaccggtga agaaagcatg
     7321 ataaccgggg acaacaaggc ttccagctac aattcgacac ctgcaaatag agcaagttca
     7381 ataaaaatta atgttgaagc agatacgagt aacccagatt caacagcaaa gagtgctata
     7441 gaagatagta tcaataaaac ggatagcgaa ccggaaaagc aaacccagga caaagctgag
     7501 aatattggaa aattggaaaa tgaacctgta aaaggatatt ttaaaatggg taaaagaagt
     7561 agcggtaaga agaagagcaa acttatacta ccagccctgt ttgtgataac atcgctggtc
     7621 gtaatcttta ttatcgccct tgttattagg catatagccc taagcccaag ctacaactac
     7681 tccgatgaag atgtaacccc agaaaacaat tataccccgg gtatttctaa catgacaacc
     7741 ccaaaaccaa aggataaaat tgacagggca tacgattccc tagtgggcca aatcaaaaag
     7801 gaagagagta acccttcaag ccctgagcct agcattgagg acaaaattga gcaggaagtt
     7861 attgaaaact acaaggctaa agatggcgag ccaacaggta aatactacct gatagttggc
     7921 agctataaaa atgccgataa tgctcggaat atggctagta agcttaaaga taaaggattt
     7981 aaaagttcgg ttgtttcaag gcctgacggt ataagttgtg ttgccctagg agcatattca
     8041 tcaaacggtg aagcggaaaa agccaagcta gcctatgaaa agcaatttcc tggaatatgg
     8101 attttgaccg tacagtagtc aattttacac atcgccggct aatttttgat gttgaaccct
     8161 cgtcaattta gagacaaaat acttcacaat gaccagtaaa tccaagccat tgtcgaatag
     8221 taaaaaacta tgtacctacc cattaattgg cgaagtttca tacattcgta aatcaggatt
     8281 aaaaagagtt tctctaagag ttaatgccta cggcgatgta cgggtatcta tgcccccatt
     8341 catctcgttt aagtatgccg aggagtttat gctctcaaac ggagaatgga ttattgaaaa
     8401 aaaaggcagg tcctttttgg ggaaaaatct tagagacaac gattcaatcc atacccgata
     8461 ccacaccatc acactatttc aggatagcca gtcaaccgga attagggtaa ttcaaagtaa
     8521 caaccaagcc aggattcact atcctttaac catggcaaaa aatgacccgg cagttagaga
     8581 agtcctaaaa aaagttgttg agcataccta tatgaaagag gcaaaagatg acctgcccct
     8641 cagaacagcc actttagctg ccgcacacgg tttctctttt cgtagggtct caataagtag
     8701 ggctactacg cggtggggta gctgctcgcc tgaaaacaac attcgtctaa gcctccactt
     8761 gatgaggctt cctgaccatt tagttgacta cgtaataatt cacgagcttt gccatacggt
     8821 ggaaaaaaat catggcatta acttctggaa gctgatgaat gagcattgta acggacacgc
     8881 gcggcaactt gccagagaga taaaagaata ccacgcgtac taggtaatct tttaatgaaa
     8941 gtggactagc acaaggatgg ttagtaatcg agcgagttta acctttcggt aagctctact
     9001 atttgggtgt aaaattcgct tgcaactttc tgatagtcaa tatttttcct acttcccttt
     9061 gcaaaatttt ttctcgaaag ctttgaaaca gtatcgcgct gcagtactat tacatcggag
     9121 atgatgtcta aaatattttc ctggttaaac tccggtgaga atgaaagctg ggtatagcat
     9181 tcattcagaa atagcttaac catgtgttta acttcctttt taaaatcacg aatcttagac
     9241 atctcaccgt aaggttatta acttcataat atttaactga ataaatatat actaaattta
     9301 tatcatagta agctaaattg aattatttta ttcctattgt gcgtaaaata ttaatgagca
     9361 aaataaaact ttagaggaaa acaaattaaa atagcttaaa tatctacttt acagataaga
     9421 taaaaggcta gtacccataa gctcttgctt caattttttc aggcaatatt tcgaaaactt
     9481 taacatttgc aagcgcagga gtgctatact taaagttctg cagggaagtg tacttcagca
     9541 taaatatctc caaaacttcc tttttcttat caatatcatc aatttccttt accataccgt
     9601 agattaatac gcttttgtaa cgcatggagt atgagcatgc aacactgtca ttttggtgat
     9661 gcatttggta gtcggtgcta aactccacac atacattcgg gttagatttc caaatgccaa
     9721 tcttcttacc ctcggggcct gaatggatgt aaagcctatc gttctgatag ccaaaattga
     9781 atggcagcac ataaggcatt ccgttggtat caaccatgcc aacatggcac accagcgagc
     9841 ggtttatgat atcattcact tcaggcgggg ttaagttgct tctcgatttc atgtctatca
     9901 aaaaaattag ctatacaacg ttacaattat ctacatgatt tacctgatat acaaagcaaa
     9961 taatggttaa aaagcactgc ttgtaaatgt ttcctaatta tcctttgaag ctgttttaaa
    10021 gcaaaaattt ataacgattg aacattatac agcagtacat tctaacactt tatcagaaac
    10081 aggttcagta caatacagta gttcatagct tatcggactt tttttaaaga aatccgctta
    10141 aaaaatctgt atgcctcagg aatccaaagc actaaagagg aggccacaaa tatttttacc
    10201 caatcggata ggctaagcgg aaccgttctg aaaatttctc caccccactg aataattacg
    10261 acttgaccta aaagtataac aaaagcaatg gtaatgaatc ccttattatg gaacagtccc
    10321 ttgagagcag aagagtaatc gccaagcgac ctggagttga ataggttcca aaattgaagc
    10381 atcacaaaga ggttgaaaaa gagggtaagc tcatagctgc taacgttgcc gtcgcggccg
    10441 aagaagataa gcatacctaa cagaataatc agaaatgatg cagaagctag tagaatccct
    10501 ttaaaaattg atggagttac gataaattcc tttgggcttc gtggaggttt atttagtacg
    10561 cctgcatggg gttgctctgt ggccagggca agagcggcaa aggtgtccat aatgaggttc
    10621 acccaaagca tttgggttac tgttaaaggg agcgtaatcc cggtaaaggg gccggtgagc
    10681 acaattacaa ctgctaaaag gttgattgtg agctggaaga agaggaactt ctgaatatta
    10741 ccataaaggg ttctacccca ccgaatggcg tttacaatgc tggaaaacga atcgtcaagc
    10801 agaatgatat cgcttgcctc cttggcaact tgtgttcctg accccatagc caaccccaca
    10861 tcggctcggt taagcgaggg cgcatcatta gttccatcac ctgtaacagc cactacctgc
    10921 ccgttttcct gtagcaacct gactagcctc attttatccg aaggacgtgc cctgtacatt
    10981 attttaagct ggtttgccat tctgcgggct tcatcttcgg aaagtgcctc aaaatcaggg
    11041 cctgaaatga catttgcttc ggcattaccg gagagaatgt ttacttgctt cccaattgat
    11101 gttgcggtaa caatattatc acccgttagc atctttacat caaccccggc cctgtggcat
    11161 tgggcaactg cttcagctgc gtcgaggcga acaggatcgg caatacccac aaccccaata
    11221 aaattaagat tggatggctt ttcgttttca ttcacaacct catggtctag aaacctataa
    11281 gcgaaagcga ttgttctgag tgatttctgc tggtagcttt caagtaaacc cgtaagcttt
    11341 gcttgtaaaa tatcgtcaac ctcacacatg cttattacaa cctcaggtgc tccctttact
    11401 agcaatattc tcttgtttaa gctgccggcc ttcccaacgg tgagcatgta ttttttttct
    11461 gacgagaaag gcagctgctg ctcaatggta aaatcatttc taatgctctg gtagttaata
    11521 ccttgattat taagccatag caggagtgca gcatcggttg ggttaccaat aacagcggga
    11581 gacttttcag taaaatcgag atgagcagta gagtttaccg ccattgaata cttaataagt
    11641 tctgcattaa gtgaattttc ggagatatcg tcaacagcgg aaaatgatga ctcaagaact
    11701 gccatacggt tctgggtaag cgtgccggtt ttatcggtgc aaacaactgt tacggcaccc
    11761 atagtttcgg tggcctgcag cttccttacc agattgttgt cggctgtcat cttacgcatg
    11821 ctgtaggcta aactaagggt aacgctcatg gcaagcccct caggaacagc aactacaata
    11881 agagtcatgg ataccatgaa ataattcaaa agcttactga taaaatcaac cgaaaaccag
    11941 tttgcactgt ctgccaggtc tatgcctttg gcaaatgcaa ttggtatggc aactaccaca
    12001 acagccaacc ctaaaagaaa ggaaagcaga tacgagttaa tctttggttt ggcaaatata
    12061 tcaggtattc cccttttggc cccgaaggct ttaactaaga aattaaggga aggtagccaa
    12121 aacttcatga gcgctaccac agctgcaact acaagcgata caagaatgaa ttgctggtta
    12181 gagctaagcc cgcttgtccc cttagcaaca tcagcaacca ccaacagaac aaatatcaga
    12241 atggctaaaa gaaaggcaat tgagccaata aaatcgctaa gccggtttag ctgcttggtt
    12301 aggggagttt gaatatcaac ctgtagggtt gattctctgg cggttttgcc aatctcagta
    12361 ctatcgccaa ctgatgttgt ttcgtaaatt ccatgccctt caacaacagc tgttcccttg
    12421 tatacccggt taatcggata ggtgttattt ccttgatcaa gtgaagaatc ataacttttc
    12481 tgcgcaattg gttctcctgt aagcgacgat tcgttcaccg acagggacac ggattcaagt
    12541 aaaacgccat cgctgggtat ctcatcaccc ctgtcaacaa taacaatatc acctactact
    12601 acatctttct tggggatgct ggttactccg ccattcctta taaccttaac cgggttttcc
    12661 tcgtttacct ggttcaaaat atcaaactcc ttccctgccc tatactcatt gataaacgac
    12721 ataaatgtgg ccagaaaaat tgccacaata atacctacac cctcaaggta atgaccatta
    12781 acagccccaa cagcaatagc tataacagcg gcaattgtta gaatcctgat aatgggatcg
    12841 ttaaattttt ccaaaagcaa ccgccaccaa ggtttacgca ctgggggagt aagcacgttt
    12901 tcaccaaact tagctctact ctcaactacc tgctcctccg ataagccttt atactttatt
    12961 tccaccataa ttcaaaaatt tttggctaaa attagcattt caatcaattc tcataggtta
    13021 atattcctta aaatttaaaa ataagcattt ttgttcaaaa ataattgaac agccaatccc
    13081 gaaatcagga tgaaatatct tggaaatcgg gttagagaaa aaattaaaaa ttaagaaaat
    13141 attttttgcc taaaagtgga gaattttcat catgtaaact aaaaaatgta ggtttgcaaa
    13201 ctttttgata gaaaacaaat agcaagtaat gggaagagca tttgagtacc gcaaagcacg
    13261 caagctaaaa cggtggggca acatggccat tgtatttacc cggttaggaa aggagataac
    13321 aatggtggca aaatcgggcg gcgcagatcc tgacaccaac cctaaactcc ggttactgat
    13381 tcagaacgcc aagtcggaaa acatgcccaa ggataatatt gaaagggcca ttaggcgtgc
    13441 aaccgataag gataccaaag aatacaagga ggtcgtctat gagggctacg gaccttatgg
    13501 ggttgccatc ctagttgaga ccgccacaga caatcctaca cgaacggtag cgaatatccg
    13561 aagctacttc aacaagcatg gaggctcgct tggaacttca ggtatgcttg acttcatgtt
    13621 tgagcataaa tgctcattca gaatagatgg taaagacagc atttcgctcg atgagctgga
    13681 gctggagctt attgactacg gggttgagga ggtgtttgtt gaggacgata gtatccttat
    13741 atttgccgac tttgcacagt ttgggccggt tcaacgttac ctggagggaa aagggctaca
    13801 tatcctaaac tttcagtttg aacgcattcc aacagatact aagaagctaa ctcccgaaca
    13861 acaagaagaa gttgagaaac tggtggataa aattgaggaa gatgaagacg ttacccatgt
    13921 ttttcataac atggaaattg cttgacctgc cctatttttc tgaaaaaatt ctacagctgc
    13981 atttcgtatg agtttcaggc ctggaaaagt aaaacaggaa cgttggactg gtaaagcatt
    14041 ttcttggaca acgatgggtt aaatagcttg tagataaggc ttctcttcac ggatgttaat
    14101 gctaccaggt taattttctt atcataaata tagtcaataa acgacttcac catatcctta
    14161 cattcaacaa tagagtattc aaccgataat tcaggattta cctggtatag gtaattttta
    14221 agagacgaca tcagcgcctt aatttcgaca ttatcggggc ggtttgtcat gttaacacag
    14281 tgaaggttga agttaaaggg tgatagaatc cttataagtt tacgaattga accaaaatcg
    14341 ctttcgtcca ggttagtagc atacaggaca tttttaacct catcaacacc cttaaggaca
    14401 gccttttcag gaattgccaa aagaggtacc tttgtttctt cagaaacctc ggaggctaca
    14461 ctaccgacca actcggtact tttaaaacct tcgccctttg tacccatcac aatgatgccg
    14521 ggtttgtacc ttttacttac ctctacaatt ccataggctg catatccctc ctgaagggta
    14581 tatccaattg aaaccgaatt gaaccccatc tgtcgggcaa attcaaccaa atcggcatgg
    14641 aatttaatga gcttttcctt ggcagctttg taaagcaagt tgaagttaat gtcaaaatct
    14701 atctgaatac ccgatacatc cgcaaaaggt atcatatcaa ctacgggaga gttaaaaaca
    14761 tgcagcaatt ttatctcggc attatacttt tcggccagca tcacggcaaa tcgggcagca
    14821 ttttgcgaag gctcggagaa atcgacaggt accaaaattt ttcgtagcgg tttttccctc
    14881 ttttttgcct cctcgctaaa cttctgctcg gatagtagcc gtaaggcttt ttcggcatcg
    14941 gactcccgga tctgaacctt aaccccttcg gaaacagctc cctgcaatat attctcgttt
    15001 cttaaaaagc actctatgcc gttagcttca aggtacgact gcaacacaac ggctttagcg
    15061 taactttcaa tggctatgac tattagcttc tcgtccatag gtaaaaagtt ttttgaaata
    15121 tacactatta agccacaaaa gtcaagaaaa aaagctaccc cctcccaggg gcagcttcta
    15181 aagaattcaa catcaggatg ctactcttct tcaagcaagc tctggataaa ggctgaaatt
    15241 tgaggcatta actcctcagc gttaacctct gtaccatctg catagatgaa ttttacggta
    15301 agttcgccgg tttcaaaatt gcgtacaaaa ctgaaatggc caatttccct atcggaggta
    15361 gtataaagtt ttacgctgat gtactcattc aggtcaacat ttgggttggt ctcatagtca
    15421 aggtcgtctg tattaaaatc ggccgtagca gtaaacttga gtgatttcat aatcagtgtt
    15481 gcgtttaact tgacgctggt gttggtttca ctaatcatgc ttacattcag gttcaagtca
    15541 agggcgtaaa gggttgtatt cccctgctta aagctaatct gcaacttgga tagagagtat
    15601 atttcggaac tgctatcgga gtaatcaata gaaacggata acttgtaacc accaaactca
    15661 gcagttaaat cgaaggattg ggttggaaag taagttgagg cataagttaa tgaaagcacg
    15721 gtaacactgc ctagctcaac ttttgccttt aaagcagtaa tttcctcata atcttcaatt
//
LOCUS       DAOPHX010000051        15763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_4242_length_15763_cov_5.629679, whole genome shotgun sequence.
ACCESSION   DAOPHX010000051 DAOPHX010000000
VERSION     DAOPHX010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 15763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15763
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4242_length_15763_cov_5.629679"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PK502_05340"
     CDS             <1..923
                     /locus_tag="PK502_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00272.1"
                     /translation="YIFSKLVPLIAKPSAMIHVATVHWNTDRWVDIQLTQLNRYLPQP
                     FRVYAFLSGDAAVHRSKYFYSSCQPVAEHAVKLNLLAETIMLLAQSDEDPLIFIDGDA
                     FPVTPLDDFIGMQLERYPLAAVQRLENVGDIQPHPSFCLTTVGFWKSISGDWLKGYEW
                     TNSRGVKRTDVGGNLLKILNDLGVEWKPLLRSGQLSEHPLMFGIYENAIYHHGAGFRQ
                     PLSMYDRLKPTSHVVKNKAVAGGIDLVLDKLPLKNRFFLQRHLGLKKNILRQNVELSE
                     AIYHDFASIISKGEAYDFISKSRRFPVTGS"
     gene            complement(948..1469)
                     /locus_tag="PK502_05345"
     CDS             complement(948..1469)
                     /locus_tag="PK502_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HPH00273.1"
                     /translation="MDTLQDFVPVSPYELKDNFFDAVDNGWMLITAGDEQKFNTMTAS
                     WGSFGILWNMPIAQVFIRPQRYTFQFVEQHSYYTLSFFDEKWRKALNFCGAKSGRKFD
                     KPKETGLVPAATGHGIAFQQARMVLECRKLYADNLKPEQFIDRDIIRKNYPKSDFHRF
                     FIGEIVNVYLARG"
     gene            complement(1480..2361)
                     /locus_tag="PK502_05350"
     CDS             complement(1480..2361)
                     /locus_tag="PK502_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HPH00274.1"
                     /translation="MNETLSKEEFSNLILKVQRDEINGFKIYSKLAKIEKDASNREIL
                     SRIAAEEYSHYNFFKSISGREVGHNRLTTWFYFTLTHLFGLTFGIKLMERQEDKAQRV
                     YPQLLPYIPEIDGILRAEEEHESELIEMIKEEKLDYAGSIVLGLNDALVELTGALAGF
                     SFAMQNTRLIAIAGLITGIAASLSMAASQYISVSTENSGQNALKSSIYTGIAYVITVI
                     ILIAPYLILQSYFAALALTLTFAILIILVFNYYISVAKDLNFKRRFSEMAIISLGVAA
                     LTFGIGFLIRKYLGVDI"
     gene            complement(2510..3268)
                     /locus_tag="PK502_05355"
     CDS             complement(2510..3268)
                     /locus_tag="PK502_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPH00275.1"
                     /translation="MKIALITGATSGIGRATALRLAQDNFNLIVTGRRLTQLEQLKKE
                     VEVKFKKEVHILNFDIRFREQVDSAIDSLPEHWRKIDVLVNNAGLALGLNHLQDGMVE
                     DWEQMIDTNIKGVLYITRKVAPLMIERGGGHIVNIGSTAGTQVYENGNVYCATKHAMH
                     ALSQAMRQDMLKHNIRVTEVRPGLLETEFSLVRFKGDKKKAKDTYAGLTPLSAEDIAE
                     VIYFALTRPPHVCINDIEVTPTAQANSYYTNRKV"
     gene            4228..5582
                     /locus_tag="PK502_05360"
                     /pseudo
     CDS             4228..5582
                     /locus_tag="PK502_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611835.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
     gene            5605..6768
                     /locus_tag="PK502_05365"
     CDS             5605..6768
                     /locus_tag="PK502_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HPH00276.1"
                     /translation="MGLRSYIDKIKPNFQKGAKYGMFHSTFDAFETFLFVPNRVTTKG
                     SHIRDSIDMKRTMTVVIVALLPALFFGMWNVGYQHALSIGESWPFWQTFFFGFLRVLP
                     LLVVSYGVGLSVEFAFAQARGHEVNEGFLVSGLLIPLILPVDVPLWMVAIATAFAVIV
                     GKEVFGGTGMNIFNPALLARAFLFFAYPSHMSGDKVWIDGLAKGQHVVDGFSGATPLA
                     NAAAYQLDKIPSAYDMFMGFIPGSIGETSKLAILIGAILLIITGIGSWKIIISVFGGG
                     LVAGLLFNTLAINPYMSIPPWQHFLMGGFAFGAVFMATDPVSASQTERGKWIYGFLVG
                     FMAILVRVLNPAYPEGMMLSILLMNAFAPLIDYYVIQGNIRRRLKRTNRVAKS"
     gene            6807..7544
                     /gene="nqrC"
                     /locus_tag="PK502_05370"
     CDS             6807..7544
                     /gene="nqrC"
                     /locus_tag="PK502_05370"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HPH00277.1"
                     /translation="MDKNSNLYTILYASGLVVLVAVLLALAATLLKPAQQRNVETEKK
                     IDILKSIGKADGVEKAANKHKFVASNFERYIVEQLVVNSLGERIQGVDAFNVDLKVEL
                     SKPLENRYLPVFVAKLDSGSTKFIFPLRGKGLWGPIWGYISLNDDFNTIYGATFSHQG
                     ETPGLGAEIATPQFQKQFDGKSIFDNQNQLVSIKVVKGGADKSDPHGVDAVSGGTITS
                     KGLEAMLYDCLSIYMPFINNQKQQSHE"
     gene            7537..8196
                     /locus_tag="PK502_05375"
     CDS             7537..8196
                     /locus_tag="PK502_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HPH00278.1"
                     /translation="MSDVKEKEPWFSAKNIELLTGPLNSKNPITVLVLGICSALAVTV
                     KLEPAIVMAISVTVVTAFSNLIISLIRNTIPSRIRIIVQLVVIAALVILVDEVLKAYV
                     YDVSKQLSVFVGLIITNCIVMGRLEAFALGNKPWPAFLDGIGNGLGYGLILVVLAFFR
                     ELLGSGTIFGYRVIPESFYYSNGGMYENNGLMILPPMALILVGLIIWIQRGRNRKLIE
                     E"
     gene            8216..8833
                     /gene="nqrE"
                     /locus_tag="PK502_05380"
     CDS             8216..8833
                     /gene="nqrE"
                     /locus_tag="PK502_05380"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HPH00279.1"
                     /translation="MENLINIFVRSIFIDNMIFAYFLGMCSYLAVSKTVKTSTGLGIA
                     VIFVMLITVPVNYLLNRFVLLPGALQWLDESFASLDLSFLSFIMFIAVIASIVQLVEM
                     VVEKYSPALYNSLGIFLPLIAVNCAIMGGSLFMQQREYETLGEATVFGLGSGVGWFLA
                     IISIAAIRERLKYSDIPKPLRGLGISFILTGLMGIAFMTFMGIKL"
     gene            8853..10112
                     /gene="nqrF"
                     /locus_tag="PK502_05385"
     CDS             8853..10112
                     /gene="nqrF"
                     /locus_tag="PK502_05385"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HPH00280.1"
                     /translation="MILLAGNSSIILGAVIVFLIVTLLLVLLLLYAKAKLTPKGKVKL
                     EINNEKVLEVEPGSSILSTLSNNKIFLPSACGGGGTCGLCRCRVVEGGGSMLPTEANF
                     FNRRQQREHWRLGCQVKVREDLSIIVPEEVLGIKKWECEVISNRNVATYIKEFVVKLP
                     DGEKLNFRSGGYIQIDVPKIEVDFSKDIFVEPEFHDDWDKTNMWSLKMKNTEETFRAY
                     SMANHPAENNIIMLNVRIATPPWDRERNTFLKVNPGVCSSYIFSRKPGDKVTISGPYG
                     DFFIKDTQNEIMFIGGGAGMAPMRSHIFHLFNTLKTNRKVTFWYGARSRREIFYEEHF
                     RKIAQEFPNFNFTIALSEPKPEDSWDGPVGFIHQVIFEQYLSRHNAPEDIEYYLCGPP
                     AMIAAVQKMLYNLGVPDEMIAFDDFGG"
     gene            10372..10608
                     /locus_tag="PK502_05390"
     CDS             10372..10608
                     /locus_tag="PK502_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00281.1"
                     /translation="MAIVKLIGITVGFLMLGMLGLGIRVIFHRSHKFPETSAGHNPEL
                     RKRGISCPKSDEQTCWGGENSYTGCSDCLERSKH"
     gene            10701..10886
                     /locus_tag="PK502_05395"
     CDS             10701..10886
                     /locus_tag="PK502_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00282.1"
                     /translation="MGTLKLILISAAMLAVVIVGLSLKLIFGKTANKSCKSNDVGYSC
                     GCGGSCGINSPNIGEQL"
     gene            11420..11713
                     /locus_tag="PK502_05400"
     CDS             11420..11713
                     /locus_tag="PK502_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00283.1"
                     /translation="MNVDSSEIYDRIKQKVQVLVVAYERAKQENEQLKAQKAELEKQL
                     KAKEQAYVELEERYSRLKLAKALKVSEADAHEAKIKVNRIVREIDKCIAFLNK"
     gene            11729..12043
                     /locus_tag="PK502_05405"
     CDS             11729..12043
                     /locus_tag="PK502_05405"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPH00284.1"
                     /translation="MEDSENKFSIRVNIADRYYPLKIDWDDEEKIRRAAKLINDKVLQ
                     YKQRYTDKDIQDFLAMASLQFVIRLIECEEKFSGAAFDEELSKLDDWLAAYIEKQNIR
                     SF"
     gene            12280..13815
                     /gene="rny"
                     /locus_tag="PK502_05410"
     CDS             12280..13815
                     /gene="rny"
                     /locus_tag="PK502_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPH00285.1"
                     /translation="MELTIIVAVIAFILGGAISYLLWNKLLASKRSKIIQDAETEAEV
                     IKKEKILQAKEKFLQLKAEHEKIVSERNSRIAQAENKIKQRETMVAQKYEELQRKQKE
                     VDAIRDTLTTQMELVDKRNEELERMHRESVEKLEALSGLSAEEAKTQLIDSLKAEAKT
                     QAMSYINEIVDEAKMTAAKESKRIVVQTIQRVATEAAVENSVTVFNIDSDEIKGRIIG
                     REGRNIRALEAATGVEIIVDDTPEAIILSAFDPVRREIARLALHQLVTDGRIHPARIE
                     EVVQKVQKQIEDEIVEIGKRTTIDLGIHGLHPELIRLVGKMKYRSSYGQNLLQHSREV
                     ANLCAIMASELGLNAKAAKRAGLLHDIGKVPDDEPELPHAILGMKLAEKYKEKPEICN
                     AIGSHHDEVEMTTLIAPIVQVCDAISGARPGARREVVESYIKRLKEMEDLALSYPGVL
                     KTYAIQAGRELRVIVGSEKVSDEEANKLSFEIARKIQDEMTYPGQVKITVIRETRAIS
                     YAK"
     gene            14016..15155
                     /locus_tag="PK502_05415"
     CDS             14016..15155
                     /locus_tag="PK502_05415"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPH00286.1"
                     /translation="MNNSSIQMCDLRGQYLKIKPEIDAAIQQVIDSTAFIKGKEVGDF
                     QDELARYLGVKHVIGCANGTDALQVALMALGLKPGDEVITPDFTFIATVEVVALLGLT
                     PILVDVDPFTFTIDTKKLEEAITPKTKAIIPVHLFGQCANMGEIMRIAQIHNLYVVED
                     AAQALGASYYHSDGHVSKAGTIGHIGATSFFPSKNLGCYGDGGALFTNDDSLAAEIRS
                     ITNHGMRVRYHHDRIGVNSRLDTVQAAILRVKLKYLDSYNEARMAAAQFYDTRLKVCA
                     NLVIPSRATWSNHIFHQYTLRVNEANREELSKFLAGKGIPSMVYYPIPLHSQKAFEAY
                     RLQNSNSTFPVSNSLSTSVLSIPMHTELDVDTINFITESILSFFK"
     gene            15185..15763
                     /locus_tag="PK502_05420"
     CDS             15185..15763
                     /locus_tag="PK502_05420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPH00287.1"
                     /translation="MEVHYFAHETAVIDSDAKIGQGTKIWHFSHIMSGSVIGENCNIG
                     QNVVVSPGVTLGRNVKVQNNVSIYSGVTCEDDVFLGPSMVFTNVINPRSAINRKNEYM
                     PTIVKRAATIGANATIVCGVTLGEFCFVGAGAVVTKDVKPYALVVGNPARQIGWMSEF
                     GHRLEFNSDNLATCPESGQVYRFENNQVNRIS"
BASE COUNT         4209 a         3365 c         3801 g         4388 t
ORIGIN      
        1 cctatatttt ttctaaactt gtacctctca ttgctaagcc aagcgccatg attcacgtag
       61 caacggttca ctggaatacc gacaggtggg ttgacattca gcttacccag cttaaccgtt
      121 acctcccgca gccattcagg gtttacgcct ttcttagcgg cgatgcggcg gtgcacaggt
      181 cgaagtattt ctacagctcc tgtcagcccg tagcggagca tgccgtaaag ctcaacctgc
      241 tggccgaaac catcatgctg ctggcacaat cggacgaaga cccgcttatc tttatcgatg
      301 gcgatgcctt tccggtaaca cctcttgatg attttatcgg catgcagctt gagcgctacc
      361 ctctggctgc agtgcaacgc ttggaaaacg tgggcgatat ccagccccat ccttcatttt
      421 gcctcaccac ggtcgggttt tggaaaagca taagtggaga ctggcttaag ggatacgagt
      481 ggactaactc caggggagtt aagcgcaccg acgtgggtgg taacctcctt aaaatactga
      541 acgatttagg tgttgagtgg aaaccgctac tgcgaagcgg gcagctgagc gagcaccccc
      601 tgatgtttgg catatacgaa aatgccattt accaccacgg ggcggggttt aggcaacccc
      661 tctccatgta cgacaggctt aaacctacat cgcacgtagt gaaaaacaag gctgttgccg
      721 gtggaattga cctcgtcctt gataagctac ccctgaagaa tcggtttttc ctacagcgtc
      781 acttaggcct gaaaaagaat attctcaggc agaacgttga gctaagcgag gcaatctacc
      841 atgattttgc ttctatcata tccaaaggcg aagcctacga ttttatttcc aaatcgaggc
      901 gttttccggt taccggcagc tgaatttttt agaatggaca gaggaagtta gcctcttgcc
      961 aggtaaacat tcacaatttc gccgatgaaa aaccggtgaa agtcgctttt ggggtagttt
     1021 ttcctgatga tatccctgtc tatgaattgc tcaggtttca ggttatcggc atacagcttt
     1081 ctgcattcaa gcaccatgcg ggcctgctga aaggctatac catgcccggt ggctgccggt
     1141 accaatcctg tttcctttgg cttatcgaac tttcggcccg attttgcccc gcaaaagttg
     1201 agtgccttgc gccatttttc atcaaaaaag ctaagggtgt agtagctgtg ttgctcaaca
     1261 aactggaagg tgtagcgttg gggtcggata aaaacctgtg caattggcat attccatagt
     1321 atgccgaagc taccccacga agccgtcatg gtgttaaatt tctgctcgtc gcctgccgtg
     1381 attagcatcc agccgttatc aacagcatcg aagaagttat ccttcagctc gtagggtgat
     1441 accggaacaa aatcctgaag tgtgtccatg caatccgaat taaatatcaa ctcccaaata
     1501 cttgcgaata aggaatccta ttccaaaggt gagggcagct actcccaggc tgattatggc
     1561 catttcgctg aagcgccttt tgaaattcaa atccttagcc accgatatgt agtagttgaa
     1621 caccagtata atcaggatgg caaaggttag ggtaagggca agcgcagcga agtaggattg
     1681 tagtatgagg tagggagcta tcaggatgat aaccgttatg acgtatgcaa tgccggtgta
     1741 aattgatgat ttcagggcat tttggccgct attctcagtt gataccgaga tgtactggga
     1801 ggccgccatt gatagcgagg cagcaatacc ggttatcaat ccggcaatgg caattagcct
     1861 ggtgttctgc atggcaaagc tgaatccggc aagcgccccg gtcagctcaa ccaaggcatc
     1921 gtttaggccc agcacgatgg agcctgcgta gtcgagcttc tcctccttaa tcatctcgat
     1981 tagctcgctt tcgtgttcct cttctgccct gagaatgccg tcaatttcgg gaatgtaggg
     2041 tagaagctgg gggtaaaccc tttgagcctt atcctcctgg cgctccatga gcttaatgcc
     2101 gaatgttagt ccaaaaaggt gggtgagggt gaagtagaac caggtggtta gcctgttgtg
     2161 tcctacctcc ctgcccgaaa tgctcttgaa aaagttgtag tggctgtact cctcagcggc
     2221 aattcgggat aggatttccc gattcgaagc atccttttct attttggcca gcttgctgta
     2281 aatcttaaat ccgtttattt catcccgttg tactttcaaa atgaggttgc taaactcctc
     2341 cttgctcaac gtttcattca tagtgcatta ggtttgagaa atattttcgt ttaaaagtga
     2401 tttcaaccct caacaatagt atgaatcaat tcttgatgta aaagtagggt aaaattagct
     2461 attttctgaa gtgacagcac gaccggcaaa aaaaagtctg agccgaagct cagacttttc
     2521 tatttgtgta gtatgaattt gcctgggcgg taggggtaac ctcaatatcg ttgatgcaaa
     2581 catgaggtgg acgtgtaagc gcaaagtaga taacctctgc gatatcctcg gccgaaagag
     2641 gggtgagacc ggcataggtg tcctttgcct ttttcttatc gcccttaaac cgcacgagcg
     2701 agaactcggt ttccagaagg cccggcctaa cctcggttac cctgatgtta tgctttagca
     2761 tatcctgcct catggcctgt gagagggcgt gcatggcgtg cttggtagcg cagtaaacat
     2821 tgccattctc gtaaacctgg gttccggccg tggagccaat gttgacaatg tgtccacctc
     2881 cccgttcaat catcaaaggc gctactttgc gggtgatgta cagaacaccc ttgatgttgg
     2941 tatcgatcat ctgctcccag tcctccacca tgccatcctg caggtgattc aaccccagcg
     3001 ccagaccggc attgttaacc aacacgtcaa tctttcgcca gtgctccggc aggctgtcaa
     3061 ttgcgctatc aacctgctcc ctgaagcgaa tgtcaaaatt caggatgtgc acctcctttt
     3121 taaacttcac ctcaacttcc tttttcagct gttccagctg ggtaagccgg cgcccggtta
     3181 ctatcaggtt gaagttgtcc tgggctagtc gtagggctgt tgcacggcca attcctgagg
     3241 ttgctcctgt gattaatgct attttcatcc gtacaaaatt ttagtattat taattagcaa
     3301 tcaattagct gcgacctggt attctacact cgttaacgtt tggataaaac ctcaatggca
     3361 gcaggtaaac aaacccctaa ttgtttagct tagcaaagct cgcaattgac gctcgcttaa
     3421 tcctcttttt taaccaggct atcctatagg ggtattccat tgttatgttg taatgctttg
     3481 catatcaata tgtagacaca attttatgta aaatttaagc ggtgtcaaac gtactgtaaa
     3541 attttgtatt gcaggctgct tttcgtcatt ttttacctat tcatgttcag tggatacagc
     3601 gtttaaaagg taaatggttt ggtttatatg gaggaagtaa tgtctggttg gctgagtctg
     3661 ggttaaaagt atatacaagt ggatgttgat gtacagcttg attagaattc tttgctaaaa
     3721 gccttgtttg tagctgctgt ggttgcagct gcctcgttta tcccggtttg gcgcagggca
     3781 tgaacttcca gtgcacttct acattcggct gcttctctag ggggccagac ttacaacatt
     3841 tagctgggaa gttttaaagg attgattgaa tgacagaaaa aaaataattt tgggaaccac
     3901 tattttttgc gaaacaattt taacggttgt attgttaatg cggtaacagt gtggggtgta
     3961 ttagaaaagt ccaaaatttc tggtataaga taataataca tctatacata ttgaaaaata
     4021 tcaatacgta tttttcgatg aatagctaaa aaggaattat ctttacattt ttttcaaagg
     4081 atagaaatat aaaataagct tggctaaata cataacctgt tgctagggaa gtttttgata
     4141 gttaattaat tgagttgagt aagcaatgga gtaaaattct cctggtatgg aatgtgaaac
     4201 tattcagcat ttaataacat actatacatg tcaactatag taaaagttcg ccgaggcttg
     4261 aatatcccta tggagggtag gcagaaaatg tgcttgttcg caaaaatatg tctgaaacct
     4321 atggagttaa acctctggat tttccagggt tgcttccaaa attggaagtt caggagggag
     4381 acgtggtgag ggctggcaca ccgctttttt atgataagta ccgtccggag gtcaagtttt
     4441 catcgccggt tagcggcgag gtggtagaga tacgccgtgg cgaaaggcgc cagatactgg
     4501 aagtagtagt aaaggcgaat ggtagtgtcg agtatcaggt gtttggagca gctcaacccg
     4561 ctgcgcttac acgcaaccag gttgttgaga agttactcgt ctctggtgta tggccttaca
     4621 ttcgccagcg tccctttgga atagtagcaa atcctaacga cacccctaag gcgattttta
     4681 tttcatgttt cgataccgcc ccccttgcac ccgatatgga cttctccata cagggtgagg
     4741 atgagaattt tcaggcggga attgatgcgc tgtcgaagct tactcctgga aaaattcacc
     4801 tggggttaaa tgccgattat cctgctgcca gtgcctttgc aaaggcaaat ggggttgagc
     4861 atcactactt cagaggacct catcctgctg gtaatgtcgg ggtgcatata caccatatcg
     4921 atcctattgg gaagggagat gttgtttggg tggttaaccc cctggatgtg gttattatag
     4981 gccgtttatt tttgaagggc atttatgatg cctccagggt aatagccctg gccggctctg
     5041 aggttaccag tccacgctac tatagggtga tttctggtgc tcaaattgat agtattacaa
     5101 gtggaaatct gaattccaac atcgaactaa ggtacattag tggaaacgtt ctaaccggaa
     5161 caaaggtaga gccgtttggg tatctggggt tttatgataa catggttacc gtgattcccg
     5221 agggtaacca ctacgagttt tttggatgga tgtctcccgg ttttaataag ttcagcgctt
     5281 cgcgtacatt cctttcgcgg ttaaccccat ggagaaaatt caaattcgat accaatttac
     5341 atggtggaaa gagagcctac gttataaccg gacagtacga aaaggttatg ccaatggata
     5401 tccttcccgt tcacctaatt aaggccatcc tggccaacga tattgagcgg atggagcagc
     5461 tgggtattta cgaggtggtt gaggaggata tggcactctg tgaatatgtt tgcacctcta
     5521 aaaccgacgt gcagcagatt cttcgaaagg gcatcgagtc gttaattaag gagctaagct
     5581 agacatttaa ttatacctca aacaatggga ttacgaagct acattgataa aattaaacct
     5641 aactttcaaa agggagcaaa gtacgggatg ttccattcta cctttgacgc ttttgaaacc
     5701 tttctttttg ttcccaatag ggtaaccacc aagggaagtc acataaggga ctctattgac
     5761 atgaagcgta ccatgacggt ggtgatagta gcgctactgc ctgccctctt ttttggtatg
     5821 tggaatgttg gctaccagca cgctctctcc attggagaaa gctggccttt ctggcagacc
     5881 ttcttttttg gctttttacg tgtgctaccc cttttagtgg taagctatgg cgtggggctt
     5941 tcagttgaat ttgcatttgc ccaagctcgt ggacacgagg tcaacgaggg atttctggtt
     6001 tccggtttgc ttattccctt gattttgccc gttgatgttc ccctttggat ggttgctatt
     6061 gctaccgcct ttgcggtgat tgttggcaag gaggtgtttg gcggtaccgg gatgaatatt
     6121 tttaatcctg cactgcttgc cagggcattc ctcttttttg cctatccctc gcacatgtcg
     6181 ggcgataagg tttggattga tgggctagcc aaagggcaac atgtagtaga tggtttttcc
     6241 ggggctacgc ctttggctaa tgccgctgcc taccagctcg acaaaattcc ttctgcctat
     6301 gatatgttta tgggatttat tcccggttcc ataggcgaaa cctccaagct ggccattctg
     6361 atcggggcaa ttctacttat tatcaccgga ataggcagct ggaagattat catatcggtg
     6421 tttggtggcg ggctggtagc aggcctgctg ttcaatacct tggccattaa tccttacatg
     6481 agcataccgc catggcaaca cttcctcatg ggtggttttg cgtttggtgc agtttttatg
     6541 gcaaccgatc cggtaagcgc ttctcaaacc gagcgtggca aatggatata cggattcctg
     6601 gtaggcttta tggccatcct ggtcagggtg ttaaaccctg cctatcctga agggatgatg
     6661 ctttccattc tgcttatgaa tgcctttgcc cctctgattg actactacgt aattcagggg
     6721 aatattcgcc gacgactgaa aagaactaat agggttgcta aatcataatt tttttcacta
     6781 tgagcatttt tggaaaattg aagagtatgg acaagaacag caacctgtac accatacttt
     6841 acgcttccgg gcttgtggtg ctggttgcag tactcctggc gttagcagct actcttctga
     6901 aacctgcaca gcaacggaat gtcgaaaccg aaaagaagat cgatattttg aagtcgattg
     6961 gcaaggccga tggggttgag aaggcagcca acaagcataa gtttgtagcc agtaattttg
     7021 aacgctacat tgttgagcag cttgttgtga actcgctggg tgagagaatt cagggagttg
     7081 atgcctttaa cgttgattta aaagtcgaat tatctaagcc attagaaaat agatatttgc
     7141 ctgtttttgt ggcaaaactt gatagcggtt ccaccaagtt tatctttccg cttaggggca
     7201 aagggctgtg ggggccaatc tggggttaca tctccctgaa cgatgatttt aacactattt
     7261 acggggctac attctcgcat cagggcgaaa cacccgggct tggtgccgaa attgcaacgc
     7321 cacagttcca aaaacagttt gacgggaaat caatttttga caaccaaaat cagcttgtct
     7381 ccattaaggt ggtaaagggt ggtgcagaca agagcgatcc ccatggagtt gacgccgttt
     7441 cgggtggcac aattacaagc aaagggcttg aggcaatgct ctacgattgc ctgagcatct
     7501 atatgccatt tattaacaac caaaaacagc agagtcatga gtgatgtgaa ggaaaaagaa
     7561 ccctggtttt cggctaaaaa tattgagctc ctaacaggtc cgttaaattc caagaacccc
     7621 ataacagtgc tggtgctggg tatatgctcc gctctggcgg ttactgttaa gcttgagcct
     7681 gccattgtta tggccatatc agttacggtg gtaactgcct tttcgaacct gattatttct
     7741 ctaatccgaa acactatccc cagccggatt agaatcattg ttcagctggt tgttattgca
     7801 gcattggtta tactggttga tgaggttctg aaggcttatg tttacgacgt gagcaagcag
     7861 ctctcggtat ttgttggtct tattatcacc aactgcatag tcatgggaag gctcgaagcc
     7921 tttgctctgg gcaacaaacc ctggcctgcc tttctcgatg gcattggaaa cgggctgggt
     7981 tacgggctga tactggtagt tttagcattt ttcagggagc tgcttggatc cggaactatt
     8041 ttcggctaca gggtcattcc tgagtcgttt tactacagca atggcgggat gtatgaaaat
     8101 aatggtctga tgattctgcc cccaatggcg cttattctgg tgggcctaat catttggatt
     8161 caaagaggtc gaaaccgtaa gctaattgag gagtaaaagc taaacaatac ggattatgga
     8221 aaatctgatc aacatatttg ttcgctccat ctttattgat aacatgattt ttgcatactt
     8281 tctgggaatg tgttcctacc tggcagtatc aaaaacggtt aaaacatcta ccggattggg
     8341 catagcggta atatttgtga tgctaatcac cgttcccgtg aactacctgc tgaataggtt
     8401 tgttctactt cccggcgccc tacaatggct tgacgagagt ttcgcttcgc tcgaccttag
     8461 ctttctttcc ttcatcatgt ttattgctgt aattgcttca atagttcagc tggtggagat
     8521 ggtggtggaa aagtactcgc cggcactcta caactccttg ggtatttttc tcccgttaat
     8581 tgcggtaaac tgtgccatta tggggggatc actatttatg cagcagcgcg aatatgaaac
     8641 cctgggtgag gctactgtat tcggattggg ctctggagtc ggttggtttt tggcaattat
     8701 tagcattgct gccattcgcg agaggctcaa gtactccgat atacctaagc ccctaagggg
     8761 tcttggaatt tcatttatcc tgacagggtt gatgggtatt gcctttatga cttttatggg
     8821 tattaagctg taaatggaaa atttttcgag ttatgatact acttgccggc aatagttcaa
     8881 taattttggg tgctgttatt gtatttctca ttgtaacgct tttactggta ttgttgctgc
     8941 tttacgccaa ggcaaagctt actcctaagg gcaaggtgaa gctggaaatt aacaacgaga
     9001 aagtactaga ggtggaacct ggtagctcca tactttcaac cctgtccaac aacaaaattt
     9061 ttctcccctc ggcgtgcgga ggaggcggaa cctgcgggtt gtgcaggtgc agagtggtgg
     9121 agggcggagg ctcaatgctg ccaaccgagg caaacttttt caaccgtagg cagcagcggg
     9181 aacactggcg cctgggttgt caggttaagg ttcgcgaaga cctgagcatc atagtgccag
     9241 aggaagtact tggaattaaa aagtgggagt gcgaggtgat ttctaaccgg aatgtggcca
     9301 catacatcaa ggagttcgtg gtgaagctac ccgatggtga gaaacttaac ttccgctccg
     9361 gcggctacat tcaaatcgat gttcctaaaa tcgaggtaga tttttctaag gatatattcg
     9421 tggagcccga gtttcacgac gactgggaca agacgaatat gtggagcctg aaaatgaaaa
     9481 ataccgagga aaccttcagg gcatactcca tggctaacca tccggcagag aacaacatca
     9541 ttatgttgaa cgtgcgcatt gccactccac catgggacag ggagcggaat acctttttaa
     9601 aggttaaccc cggcgtttgc tccagctaca tattttcccg aaagcctggc gataaggtca
     9661 ccatttccgg gccatatggt gattttttta ttaaagacac acagaacgaa attatgttta
     9721 ttgggggagg agccggaatg gcccccatgc gttcgcatat ctttcatctg ttcaacacgc
     9781 tcaaaaccaa ccgtaaggtt accttttggt acggagcccg ctccaggcgc gaaatattct
     9841 acgaggagca cttccggaag attgcccagg agttccccaa ctttaacttt accattgccc
     9901 tgtccgagcc caaacccgag gatagctggg atggccctgt ggggtttatc catcaggtta
     9961 tatttgagca atacctctcc aggcacaatg cccccgagga cattgagtac tacctttgcg
    10021 gacccccggc catgattgct gccgttcaga aaatgcttta caaccttgga gtccccgacg
    10081 agatgattgc ttttgatgat tttggaggat aaccagcctt taaacttatt ggccgaacta
    10141 tagcatgaga cacatgatat ccggatttct aaaaaaaacc tgttgttaaa caattgtttc
    10201 ttatataccc tcagtgtttc attgcaaagc agccgttctt ttggatgaaa ataacaaaat
    10261 gcagtagaaa tcctacttac gaacaacttg taattagaat gccgaaaacg tcaacggtaa
    10321 agtcaggcac ctaaactcaa ccattggctt ttcaggaaaa gtagtaacgt gatggcaata
    10381 gttaaactaa taggaattac ggtcggattt ctgatgctgg gtatgctagg cttgggaatt
    10441 agagtaatct ttcatagaag ccacaaattt ccggaaactt cagccggtca taacccggag
    10501 ctaagaaagc gtggaatatc ctgtcctaaa agcgacgaac aaacgtgctg gggcggagag
    10561 aatagctaca caggttgttc tgattgcctc gaacgttcaa aacactgaag ctgtgttaga
    10621 atacctaatg caagtttaac cttaatgaga aacaatttga attttttaaa ataaacttat
    10681 tacgagtagg tgatatcatc atgggaacat taaaattgat acttatttca gctgccatgc
    10741 tggcagttgt gattgtggga ctttcgttga agttgatttt cggaaaaaca gccaacaaat
    10801 cctgcaaaag caacgatgtg ggctacagct gcggatgtgg tggaagttgt ggcatcaact
    10861 ctccgaatat aggcgaacag ctataagttg ttcattagtg ttcggattat ccggattcat
    10921 aaaatacatt ctgttgcatg tgcaacttct ggggtttatg gttttattta cttccccgaa
    10981 gcagccaata caccaacttt tactgccgga taaacggatc tgtctatttc ggattattgt
    11041 ttaatgcgct gatatatagt ttattaatgt gtataattta agaattgaag catttttcga
    11101 cctaaagcta cccattgtaa tcctcgctgt actgtatcgc atttcgtctg gtaacatgtt
    11161 tcaaacctga acaattgctg gcgtaggcaa gttggctata aatgattaaa aaacctaatg
    11221 cctgtaggta atccttctca cttatattct cccggaattt ggactgcacc taagcaacgc
    11281 caaatagtag gctgtagcca atggacattt taacttatcc atagtttcct gctaatttga
    11341 catttacgtt aggtttatgt tgcaataatt ttgcattttg gaaaatctat agctaacttt
    11401 gtagaactta aatgtggaaa tgaacgtcga ttcaagcgaa atatatgaca gaattaagca
    11461 aaaggttcag gttcttgttg ttgcatacga gcgagctaaa caggaaaatg aacagcttaa
    11521 ggctcagaaa gccgaactcg aaaaacagct gaaggctaaa gagcaggcat acgttgaact
    11581 tgaagaacgt tattcccgtt taaagctggc taaggcgctt aaggtttctg aagctgatgc
    11641 gcatgaagcc aaaattaaag tgaacagaat tgtgcgggaa attgacaagt gtattgcttt
    11701 tcttaacaaa taggccagat acagttaaat ggaggatagc gagaataaat tctcaattag
    11761 agttaacata gccgataggt attatccctt aaaaattgat tgggatgatg aggagaaaat
    11821 acgcagggcg gctaagttaa taaatgataa ggtactgcag tacaagcaac gatacaccga
    11881 taaggatatt caggattttt tggctatggc atccttgcag tttgttatca ggctaattga
    11941 gtgtgaagaa aaattctccg gcgctgcttt cgacgaggag ctctccaagc tggacgactg
    12001 gttggccgca tacattgaaa agcaaaacat acgttctttc taatagcatt gcccgcatta
    12061 ctgctctaat attttgcgaa actcaacatt actaaatttg gagttggact ttattcgata
    12121 aggacaggcc tttttaccgc ttagctcggg attcctttag tagggacgtt ggaagtccga
    12181 cccggatggc cgctccaccc atgtaaatct ggggtttcta cataaagtta gagtgtaatg
    12241 cgggtttttt tatattaacc cactaataaa taatatgtta tggagttaac aataatagtt
    12301 gccgtcatcg ccttcatttt aggtggtgct atatcatacc tgctatggaa caagctgctg
    12361 gccagcaaga gaagtaaaat catacaggat gctgaaaccg aagctgaagt tattaaaaag
    12421 gagaaaattc ttcaggccaa ggaaaaattt ctgcagctca aggcggagca cgaaaaaatt
    12481 gtatccgaaa ggaactcaag gattgcgcag gccgaaaata aaattaagca gcgcgagaca
    12541 atggtagccc agaaatatga ggagctgcaa cgtaagcaaa aggaggttga tgcaattcgg
    12601 gacaccctta ccacccaaat ggagctggtt gataagcgaa acgaggagct ggagagaatg
    12661 cacagggaat cagttgaaaa gctggaagct ctttcgggtc tttcggctga ggaggccaaa
    12721 acccagctca ttgactccct taaggctgaa gccaaaactc aggccatgtc gtacatcaac
    12781 gagattgttg acgaggccaa aatgactgct gccaaggaat caaaacggat agtggttcaa
    12841 accattcaac gtgttgcaac cgaagcagca gttgaaaatt cggttaccgt gttcaacatt
    12901 gactccgatg agattaaagg gcgtatcatt ggccgtgagg gccgaaacat ccgagctctc
    12961 gaagcagcca cgggcgttga aattattgtt gacgataccc ccgaagccat catcctttcc
    13021 gccttcgacc cagttaggcg cgagattgcc cggctggcgc ttcaccagct cgtaaccgac
    13081 ggccgcatac atcctgcccg aatcgaggag gtggtgcaaa aggttcagaa gcagatagag
    13141 gatgaaatag ttgaaattgg aaaacgtacc accatagacc tgggcattca cgggcttcac
    13201 ccggagctca tccggttggt tggcaagatg aagtaccgtt cgtcgtacgg ccaaaacctg
    13261 cttcagcact cccgcgaggt ggccaacctt tgcgccatta tggcctccga acttggcctt
    13321 aatgctaagg ctgccaagcg tgcagggttg cttcacgata ttggcaaggt tccagacgat
    13381 gagcccgagt tgccacatgc cattctggga atgaagctgg ccgaaaagta caaggaaaaa
    13441 ccggagattt gtaacgctat tggctctcat cacgacgagg tggagatgac aacccttatc
    13501 gcacccattg ttcaggtttg cgatgccatt tcaggagcgc gcccaggcgc ccgaagagag
    13561 gtagtggagt cttacatcaa gcggcttaag gagatggagg atcttgcgct ttcctatccc
    13621 ggcgtactga aaacttatgc cattcaagcg ggtagagaac tacgggtaat tgttggcagc
    13681 gaaaaggttt ccgatgagga ggccaacaag ctttccttcg agattgcccg taaaatccag
    13741 gatgagatga cctatcccgg tcaggtgaaa attaccgtta tccgcgagac cagggctatt
    13801 agctatgcaa agtagcagcg aaatcttctg gctgctagta ttcacacttt gtccattccc
    13861 atttttttta taaggcagat tcataagtga tggccttatg ggatggccat tttgaaatga
    13921 gttgcagcaa tgattcgaaa gggcaatatt atggtttgac tttcggcacg ataggggtaa
    13981 taagctattc ataaatacat gaaaaatata ccggaatgaa caattcatca attcagatgt
    14041 gcgaccttcg agggcagtac ctgaaaatca aacccgaaat tgatgctgcc attcagcagg
    14101 ttatagatag cacggctttc attaagggta aggaggtagg ggatttccag gacgaacttg
    14161 cccgctatct gggggtaaag catgtaattg gatgcgccaa tggcaccgat gcccttcagg
    14221 ttgcccttat ggcgttgggg cttaaacccg gagatgaggt tattacccct gactttacat
    14281 ttattgccac cgttgaggtg gtagccttgc tggggttaac gccaatctta gtggatgttg
    14341 acccatttac ctttacaatt gatacaaaaa aacttgaaga ggcaataacg cctaaaacaa
    14401 aggccattat tcctgttcac ttgtttgggc agtgcgccaa catgggcgaa ataatgcgta
    14461 ttgcgcaaat tcacaacctc tatgtggtag aggatgcagc ccaggcactt ggtgccagct
    14521 actaccattc tgatgggcat gtttcaaaag ccggaacaat tggccatata ggcgctacct
    14581 cgtttttccc atcaaaaaat cttggatgct atggcgatgg cggtgcgctt tttactaacg
    14641 atgattcgct ggcagcggag attcgttcca ttaccaacca tgggatgagg gtacgatacc
    14701 atcacgacag gattggagtt aactccaggc tcgacaccgt tcaggcagcc attctgcggg
    14761 ttaagctgaa gtacctggac agctataacg aagcgcgaat ggccgcagct cagttttacg
    14821 atacccgcct taaggtatgt gcaaacctgg ttatcccgtc aagggctaca tggagcaacc
    14881 atatctttca ccagtacacc ctaagggtta acgaggctaa ccgcgaggag ctgtcaaaat
    14941 ttcttgccgg caaaggtatt ccctcaatgg tttactaccc aattcccctt catagccaaa
    15001 aggcttttga ggcttacagg ttgcaaaact caaacagcac atttccggta tccaactcgc
    15061 tatcaactag cgttttatcc attccaatgc acaccgagct ggatgttgat actattaatt
    15121 ttattaccga aagcattttg tcattcttca aataagctac ttttacattc aaaattttta
    15181 caggatggaa gtccactatt tcgcgcatga aacagcggtt atcgacagtg atgcaaaaat
    15241 tggacaggga acaaaaatat ggcattttag ccatattatg tcgggttcgg taataggtga
    15301 gaattgcaat attggccaaa acgtggtggt gtctcccggt gttaccctgg gccggaatgt
    15361 taaagtgcag aacaacgtat caatttattc aggggttacc tgtgaggatg atgttttttt
    15421 aggcccctca atggttttta caaatgtgat aaatccccgc agcgccatca accgaaaaaa
    15481 tgagtacatg cccacaattg ttaaacgagc agccaccatt ggtgctaatg ccactattgt
    15541 ttgtggcgtt accttgggtg aattttgctt tgtaggggca ggagcggttg taaccaaaga
    15601 tgtgaaaccc tatgcgctgg tggtgggaaa tcctgccagg cagattggct ggatgagcga
    15661 gtttggccac aggctggaat tcaatagtga taaccttgct acctgccctg agagcggaca
    15721 ggtttatcgt tttgagaaca atcaggttaa taggattagt taa
//
LOCUS       DAOPHX010000052        15730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_4254_length_15730_cov_6.519107, whole genome shotgun sequence.
ACCESSION   DAOPHX010000052 DAOPHX010000000
VERSION     DAOPHX010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 15730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15730
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4254_length_15730_cov_6.519107"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..1054
                     /locus_tag="PK502_05425"
     CDS             11..1054
                     /locus_tag="PK502_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPH00288.1"
                     /translation="MYRLFLRNVLAVFLILVALGYSEAQEKKAYKVSCIGFYNLENLF
                     DTLPGPNDVEFTPDGPNKWNSERYYHKLNNMAEVIAQIGDEMFPGGPAVLGVSEIENR
                     LVLDDLVNTPRLKQSNYQIVHYDSPDRRGVDVGLLYRPEFFKVTSHRSVRLNIPDNPE
                     FRTRDQLVVSGLLDGETIHIIVNHWPSRSGGEKRSQPYRIAAAKLSRSIIDSIRATDA
                     DAKIILMGDLNDDPDSRSIIDYLGAKNNAEGLRSGDLFNVMASLYREGIGTLAYQDSW
                     NLFDNIIITQPLLSKDRTSWVFYKAKVFNKPFLRQTSGQYAGYPFRTYVGANYSGGYS
                     DHFPVYIFLIKEK"
     gene            1353..2831
                     /locus_tag="PK502_05430"
     CDS             1353..2831
                     /locus_tag="PK502_05430"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HPH00289.1"
                     /translation="MKKIPEVSNRFFSTNLFLLLLLNLIVKPFWIFGIDRSAQNALGA
                     SDYGLYYALFNFTLIFNILLDCGVTNFNNREISFHPQLAGKYFANILSIKLMLSVVYV
                     AITVAVALGMGYGHHQLGLLFILAVNQFLSSLVMFIRSNLNALHHFGLDSVLSVLDKL
                     IMILLGSVLLWTPLRKNFTIEAFAVAQTLSYMATVAVSLIFLTQTTGRLRIKFDRVLS
                     LSMLKNSLPYALMVLLMTAYSRLDSVVIDRLLPNGSEAAGIYAQAFRLLDAANMLPFL
                     FASLLLPIFSGMIKQKREFGPFLNFSFLLLVVPVVAIAIPTIAHRAPIINLLYHEHAD
                     LSAPVLGLLMISYIFISVGYVFGTLLTANGNIKELNIVSGAAVVVCLSVQITLVPRFG
                     VTGAAIGNMVVNFLVAVAQVTISLKKFRLTPDRTMVFRFLAYLLFCPLATVSILNLHL
                     GMAFSYVLSIAVSLGFALVLKLIDIKQLLATFTTENTPNVKG"
     gene            3000..3512
                     /locus_tag="PK502_05435"
     CDS             3000..3512
                     /locus_tag="PK502_05435"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPH00290.1"
                     /translation="MGILVEKSADCPYINFSEDGILEIEGRSITEDPFTFWQPLLEWV
                     ETYTKHPSPKTEVIIYMEYSNSSSNKYINELLRKLEECHRKTSSVRVTWRYEQDDESI
                     YQLGRDFESMLHVPFEFIEVNTSADNSKRIRVKNKSNGSEAIITHRYWEAIVRNGHGE
                     DYQILQEFSN"
     gene            3552..3941
                     /locus_tag="PK502_05440"
     CDS             3552..3941
                     /locus_tag="PK502_05440"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPH00291.1"
                     /translation="MLKLELKETATTPYINFDPTSGVIRMEGRSIPENVIDFYQPILG
                     WVEEYGKNIQPETTVHLKFEYFNTSSSKRIFDLMKKFEKIGIETGTKVTINWYYEEDD
                     EDIFFAGNDYKALINKVEFNLIEIKPS"
     gene            3994..4434
                     /locus_tag="PK502_05445"
     CDS             3994..4434
                     /locus_tag="PK502_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HPH00292.1"
                     /translation="MTLNEVQDAIVEDFSISNDWMDRYQQLIELGKELPPIDEKKRTD
                     QYLINGCQSKVWLDAELRDGRIFFSADSDAIITKGIVSLLIKVLSGRTPEEIINADLY
                     FIDKIGLRENLSPTRSNGLVAMVKQMRMYALAYQTKLNQQKDNH"
     gene            4626..4913
                     /locus_tag="PK502_05450"
     CDS             4626..4913
                     /locus_tag="PK502_05450"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00293.1"
                     /translation="MLEKKLYANGQPVYKLADGMLIFYYKNGTVKAEGPYPDNMMEGE
                     WRFYRESGQLWQIGNFKNNQKHGSWIRYDRNNNVEYQETFEHNKAVNPKRR"
     gene            5140..5862
                     /locus_tag="PK502_05455"
     CDS             5140..5862
                     /locus_tag="PK502_05455"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HPH00294.1"
                     /translation="MRRFMITGTFALMTSMGSYAYAQGQVTIQNDSKDFVGVKNSISA
                     TVNLYQASTYKVEITTSQKVADKLEISVENGLLSIREKDQSWNHWSCSNNTDDEVVVN
                     VWSPKYSTLEINGSGDMLAKTPIKTNEIEVKVNGSGDVKINELEAEKVSLKTNGSGDI
                     AVSGKKEASTLVIKINGSGDIDTRNLVTRVADVAITGSGDVSANVLEKFTANSVGSGD
                     IYLSGNPQVDAKILGSGEIVRK"
     gene            complement(5978..7183)
                     /locus_tag="PK502_05460"
     CDS             complement(5978..7183)
                     /locus_tag="PK502_05460"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HPH00295.1"
                     /translation="MLSIREVYKEGYGPSSSHTIGPVRAAELFIKKHPEVSYFRVMLF
                     GSLALTGRGHQTDSALRKCFLPKSVEVVFKPEETLPLHPNGMVFEALTDEGAEALDRW
                     EVYSIGGGDLKDKSGVLNSSEVYSLNNMTDILTWCRREGKTFWEFVEDNEGKDIWEFL
                     QGVWHTMRETIKRGIENEGVLPGPIKLARRASSQYSKALTSHGTLKSFGLLFSFALAV
                     AEENAAGGRIVTAPTCGSSGVLPAILFYLSREEYLTEVKIHRALATAGLIGNLVKHNA
                     SISGAEVGCQGEIGTACAMAAAAMVQVLGGSPNQIEYAAEMGMEHNLGLTCDPVLGYV
                     QIPCIERNAIAASKAYSCGVYALSSDGGHKISFDRAVETMAETGRDIQVAYRETGIGG
                     LAKNWEPFE"
     gene            7459..9246
                     /locus_tag="PK502_05465"
     CDS             7459..9246
                     /locus_tag="PK502_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPH00296.1"
                     /translation="MKPIIQATSISTVTYISNLLIGLTIALTQFFTAEAYSQDTLKTY
                     CNPINIDYGYSPIPAAIEQVKHRATADPLVVAFKGKFFLFSTNQHGYWWSSNMASWNF
                     IPRKFLLPRHKVYDDLCAPAAFVLGDSLLLIGSSNNPDFPIWASTNPAADDWHIAIDS
                     FPLAAWDPAFFADDDGRVYLYFGSSNLYPIYGCEVNRATLRPQAAPKPLISLNDSIHG
                     WERFGEYNDNTFLPPFIEGAWMNKHNGHYYLQYAAPGTEFSGYADGVYIGSNPLGPFR
                     YQEFNPFSYKPGGFARGAGHGATFCDYNQQWWHISTIAIGVKNNFERRIGMWPAYFDA
                     DGTLYSQTAFGDYPHYLNGKFTEWMLLNYQKPVTVSSTLYGYYPNFAVDEDIRTYWSA
                     STGNAGEWICTDLLDTCRIYAFQVNYADQDVQLVGKQMGIYHQYRVWGSLDGKSWSLL
                     IDKSENKTDVPHDYVELSTPVEFRFLKLENLHVPAGKFAISGFRVFGKANLPLPESVE
                     FFSVLRGNSEPRNAWLKWKLQDNATGYLINFGVRPDKLYGSIMVYGHNEYYFSGMDRD
                     KTYYFTITPFNESGCGNRSAVVKSEIPLR"
     gene            9342..10097
                     /locus_tag="PK502_05470"
     CDS             9342..10097
                     /locus_tag="PK502_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPH00297.1"
                     /translation="MNSWFKSLSASIAKHRVGILTTIIIHLLVIASFLVFKIEDRTEE
                     YASTIEMEFEEPKAQEELLVEEKPEQLLPTDVTVPSREMEAIKNFAVDATQKELNAGL
                     SDEKNTDADNLYSEANRIREQMQENKERYEQAMKEADIAIPNTPEKNIPEEKKNMYKG
                     PTVISYNLAGRKAYRLPVPSYKCEMGGQVVVNIEVLSDGSVSNATIDWANSVNDECIN
                     NSAIMAALASRFSSTQQSTGKEKGSITYLFVAQ"
     gene            complement(10112..11332)
                     /locus_tag="PK502_05475"
     CDS             complement(10112..11332)
                     /locus_tag="PK502_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HPH00298.1"
                     /translation="MKRTASNLPEFPHAKGLLVQEERLEFVKKNKQLVIGIPKEADST
                     ESRIPLTPEAVEMLVEGGHEIIIEKDAGKQANYNDLDYSEKGGRIVTSSQEVYRSDIV
                     LKVAPLIPAEFELVRDNQIIMSSLHLNSSTGDYLRKLIQKKVTAIAFESICDSDGSFP
                     FIRAMSSIAGSTSVLVAAEYLSNVNKGKGVMLGGITGISPTEVVILGAGTAAEFAARA
                     ALGLGATVKVFDNSIKRLMELQNLLGQRLYTSIFHPQVLARVLKTADVVIGTLTPEES
                     NLRFVVSEEMVMNMKHGSVIVDLAIDKGGCFETSEIRDHNNPSFEKYGVVHYCVPNIT
                     SRVARTASIAMSNVFAPLLLSIGEAGGFQHQLKEDSGLRHGVYVFNGILTNDYLGRLY
                     NLPSRDINLLMAAF"
     gene            complement(11638..12066)
                     /gene="tsaE"
                     /locus_tag="PK502_05480"
     CDS             complement(11638..12066)
                     /gene="tsaE"
                     /locus_tag="PK502_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPH00299.1"
                     /translation="MKSLLIPTIEALDSVASEVAEIVKQYHVVCFYGEMGAGKTTLIK
                     AICHALGVKDIVASPTFALINEYQTRSGQPVYHFDFYRINRIEEVYDFGYEEYFFSTT
                     GICLIEWPELVEDILPSDQTVKLFITVNPDFSRRLDIMVD"
     gene            complement(12290..13849)
                     /locus_tag="PK502_05485"
     CDS             complement(12290..13849)
                     /locus_tag="PK502_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HPH00300.1"
                     /translation="MATPASILWVDDEIDLLGSHIIFLEQRGYHVQKAINGVDAIELV
                     RNNVFDIVFLDENMPGLSGLETLTQIKELKPHLPVVMVTKSEEENIMDMAVGSQIADY
                     LIKPVNPNQILLSIKKHVHGKTLVSAKQTSDYGVEFSQLSEQIAAAQSYSDWVELYRR
                     LSAWQLRISDNSEPNLRQMLDMQMAEANQLFSRYVRSSYIGWFKGNQDDKPLLSPNLL
                     RSKVFPLLDSGEKVLLVLIDNLRYDQWKAIEPLLADEWRVESDDVYFSILPTATQYAR
                     NAIFAGLMPLEIQQMFPDIWISDDDDEGKNNYELQLLTSQLQRLGKSYKVHYEKGNTL
                     RAGMPIADNVNLVLNNNLTVLVINFVDMMSHARTDSNMMKELASDEAAYISLVRSWFQ
                     HSGLRNILLEAARFDIKVVITTDHGSIRVQNPIKVVGDRATSTNLRYKLGRSLAFNTK
                     EVFEVRDPETAHLPKPNVSSSFIFAYGSNFFVYPNNYNYFASYYRNTFQHGGISLEEM
                     IIPVVVLTPNR"
     gene            complement(13859..>15730)
                     /locus_tag="PK502_05490"
     CDS             complement(13859..>15730)
                     /locus_tag="PK502_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00301.1"
                     /translation="YDREMRMGPPYEMAFSIRIQLIALSNMKGEDLKRELLKLASDEK
                     NKDYLDQLYYTLGNMEKQEGNMDKAIEYYLLSARLSTTNDNQKGMSYLVLADHYFQKS
                     EYAKAQAYYDSSFNSLDASFPGYAQLETKTRFLTNLVENLNIVSTEDSLQRVAAMSPK
                     DRDALIASIIGKLQEEEQKLKAQEQADQMQSQIYMQNQRFRSNSEPQGGKWYFYNPAS
                     LSYGQSEFQMKWGKRKLEDNWRRKNKGVSDFENIASGGLPSDSAANPQKQLSNTSPEF
                     YLVNLPFTDSLKQISNQKILTAMMRIAEIYQTDLNDSKAAKESYKKLSERFPDASEAA
                     TAIYRMYRIAADEGNSVEAEGYKIALLSRFPSSPYALILSNPEYIKELTVKENEANSI
                     YETAYGLYLQGNFSEAAKTLKSGMGKVKGTDMEPKYLLLDALCQSKTGDLRTFKESLE
                     QVALTFPKSEQGQVAQNLLTYLEQRDLQLSTGKVSIDVPVVSVSSDSISTSIYTKPEG
                     EHAFVLLVPKRTNINQLKFNIITFNVDNFIEMDLALNSVPLNDFVEVVSVTGFQSADI
                     ALSYYKLITQDEKVFADFNRAEFQAFLFSTENFNRFMNDKSIPDYSRFFKENYSVDN"
BASE COUNT         4317 a         3701 c         3505 g         4207 t
ORIGIN      
        1 tcacttgatt atgtatagat tatttctaag gaatgtgctg gcagtatttc tcattcttgt
       61 ggcgttgggt tattccgagg ctcaggaaaa aaaagcctac aaggtaagct gcataggttt
      121 ttacaacctg gagaatcttt ttgatacgtt gcctggtcct aatgatgttg aattcacccc
      181 tgatggccct aacaagtgga attccgaacg ttactaccat aagcttaaca acatggcaga
      241 ggttatagca caaataggtg acgagatgtt ccccggaggg ccagcggttc tcggggtcag
      301 cgaaattgaa aaccggttgg tgctggacga cctggtaaac actcctaggt taaaacaatc
      361 gaactaccaa attgttcact acgactcgcc cgacagacga ggcgttgatg tgggtttgct
      421 ataccgcccc gagtttttca aggttacctc ccaccgatcg gtcaggctga acattccaga
      481 caaccctgaa ttcagaacca gggatcagct ggttgttagc ggcttgcttg atggcgaaac
      541 cattcatatt attgttaacc actggccatc gagaagtggt ggggaaaaac gtagccagcc
      601 ataccgaatt gcagctgcta agctctcccg ctccatcatt gactcaattc gagcaaccga
      661 tgctgatgcc aaaattatcc tgatgggcga cctgaacgac gatcccgaca gcaggagtat
      721 catagactac cttggggcaa aaaacaacgc tgaagggctg aggtcaggag accttttcaa
      781 cgttatggcc agtctttacc gtgagggaat tggaactctt gcctaccagg actcttggaa
      841 tctttttgat aatatcataa tcacacagcc cttgcttagc aaggacagaa ccagctgggt
      901 attttataag gcaaaggtgt tcaacaaacc atttttaagg caaactagcg ggcaatatgc
      961 tgggtaccct ttcagaacct atgtaggtgc aaattactcg ggtggctact ccgaccattt
     1021 tcccgtttac atattcctta tcaaggaaaa gtagcaaact tgtttaatca gcacccctgc
     1081 caggtataac cacgcatcag caggtcaagt gcaggaaaat ctcccagatg aaacctgctg
     1141 ccagcaccaa gtatgctaca taccctatct tttagccata tgttggcaat aatagtttcg
     1201 gtatgaagtg aacgcaagca taatggtagc tgaattatag ccagataggc ctcggccaca
     1261 tcaacattgc cgggctctat cctatttatt cattttggca aaatattctt taaagcattc
     1321 acgtttaggt atcatttaaa gcgtagccgg atgtgaaaaa aatacccgaa gtttccaacc
     1381 gcttcttttc aaccaacctg tttttactac ttctgcttaa cctgattgtt aagccgttct
     1441 ggatttttgg catagacagg tcggctcaaa atgccttggg ggcaagtgat tacgggttgt
     1501 actatgccct gttcaacttc accctaattt tcaacattct actcgattgc ggggtaacca
     1561 acttcaacaa cagggaaata agctttcatc cgcaacttgc cggtaagtac ttcgccaaca
     1621 tcctttccat caagctaatg ctgtctgtgg tgtatgtggc gataacagtg gctgttgcac
     1681 tgggtatggg atatgggcat catcaacttg gcctgctatt catactagca gtcaaccagt
     1741 ttttatcgtc cctggtaatg tttataaggt caaacctcaa cgcccttcat cattttggcc
     1801 tagatagcgt gctgtcggtg ctggataagc tcatcatgat tctgctgggc tcggtgcttc
     1861 tttggacccc cctcagaaag aatttcacca tagaagcatt tgctgtagct caaaccttgt
     1921 cgtacatggc aaccgttgcg gtatcgctaa tatttcttac acaaacaacg ggcaggcttc
     1981 ggattaaatt cgacagggtt ttatccctta gcatgttaaa aaatagcttg ccatacgccc
     2041 tgatggtttt gctgatgacg gcctacagcc gtttagactc agtggtaata gaccggctgt
     2101 tgcccaacgg cagcgaagcg gcaggaatat acgctcaggc attcaggctg ctcgatgccg
     2161 ccaatatgct tccatttctt tttgcttcac ttttactccc aattttctca ggcatgatca
     2221 agcaaaaaag ggagttcggc cctttcctta acttttcctt cctgctactg gtggtgccgg
     2281 tagtagccat tgcaattcca acaattgccc acagggcgcc aattataaac cttctctacc
     2341 acgaacatgc tgatttgtcc gctccggttc tggggctgct tatgatttct tacatcttca
     2401 tttcagtggg ttacgtgttc ggaacactgc ttaccgcgaa tgggaatatc aaggagctca
     2461 acatcgtttc aggagctgcc gttgttgttt gcctttctgt ccagataacg ctcgtgccca
     2521 gatttggggt aactggtgcg gcaattggca acatggtcgt aaacttcctg gtagctgttg
     2581 cacaggttac tatttccctc aaaaagtttc gcctcactcc tgacagaacg atggtttttc
     2641 gctttttagc ctaccttcta ttttgtccac tggccactgt ttccatttta aatctgcatc
     2701 tgggcatggc tttctcgtat gttttgtcca ttgcggtatc tcttggtttt gccttagtgc
     2761 taaagctaat tgatataaaa caacttctcg ccaccttcac cactgaaaat actcccaacg
     2821 tgaaggggtg aactcatcat ttttcattta ccggttcatt attgaagcat acgaaaactc
     2881 ataaggatac acgctgccac aaaacattag tagtgatgga cagcttcctt aaagaaattt
     2941 tgcacattta aaaacaaact tatatcttta tgggctttaa gaaattcagg taaataacga
     3001 tgggcatact agttgaaaaa agtgccgatt gtccgtatat caatttttcg gaggatggga
     3061 ttctggaaat agaaggaagg tccattaccg aagacccctt taccttttgg caacctcttt
     3121 tagaatgggt tgaaacctac acgaagcatc cgtctccaaa aactgaggtg attatttaca
     3181 tggagtactc caacagtagc tccaataagt acatcaacga gctactacga aaactggagg
     3241 agtgccatcg caaaacatcc agcgtacggg ttacctggcg gtacgaacag gacgacgagt
     3301 cgatttacca gctcggaagg gattttgaat ccatgcttca cgtcccgttt gagtttatag
     3361 aggtgaacac cagcgccgat aactcaaaac ggattagggt taagaacaaa agcaacggta
     3421 gcgaagctat aattacccat agatactggg aagctattgt acggaatggg catggcgagg
     3481 actaccagat acttcaggaa ttttccaact agcataattt ttgcatattt gccccaataa
     3541 aaattttgta gatgctaaaa ctcgagctca aggaaaccgc aacaaccccc tacatcaact
     3601 ttgaccctac aagcggggtt ataaggatgg agggcaggtc gattcccgaa aatgttattg
     3661 atttctacca gcccattctt ggatgggtgg aggaatacgg taaaaatatt caacccgaaa
     3721 ccaccgtcca tttgaaattc gagtacttca acaccagctc ctcgaaacga atttttgacc
     3781 tgatgaaaaa gttcgaaaag attggcattg aaaccggtac gaaagttacc atcaactggt
     3841 actatgagga ggatgacgaa gacatatttt ttgcaggcaa cgactacaag gcgctcatca
     3901 acaaggtgga gttcaacctg attgagatta aacccagcta aaagcatttc gttctgttta
     3961 caagcacgca tacaacaaaa taatccacat acaatgaccc ttaatgaggt tcaggatgcc
     4021 atagttgagg atttcagcat ttccaacgat tggatggatc gctaccagca gctcatagag
     4081 ctgggtaagg agctgccccc aattgacgaa aagaaacgca ccgaccagta cctgataaac
     4141 gggtgtcaga gtaaggtttg gctggatgcc gagctgcgtg acggaaggat tttcttctca
     4201 gccgacagcg atgccataat caccaagggg atagtgtcgc tgctcatcaa ggtactctcg
     4261 ggtagaactc ccgaggaaat catcaacgcc gacctatact tcattgacaa aattggatta
     4321 agggagaacc tctcacctac ccgcagcaac ggccttgtgg ccatggttaa gcagatgcgc
     4381 atgtacgcac tcgcctacca aaccaaactt aaccagcaaa aggataacca ttaacaactg
     4441 gggaagccta ttaattctcc caccacaggt tgacttagca cgatgaactt gaactcagct
     4501 gatgctggtg ggtaaaagtt ctggcgatta atccgatagc ctgttgttta cgctatactc
     4561 atctttctct ttttaaaagt tccatttata acttgaatcc atcatcaata aaaaagttta
     4621 caccaatgct cgaaaaaaaa ctttacgcta acggacagcc tgtttacaag ttggccgatg
     4681 gaatgctaat tttttactac aaaaacggga cagtaaaggc tgaagggcca tatcctgaca
     4741 acatgatgga gggagaatgg cgtttttaca gggagtcggg tcaactttgg caaatcggaa
     4801 atttcaaaaa caaccagaaa catggttcct ggatacggta tgacaggaat aacaacgtgg
     4861 agtaccaaga gacttttgaa cacaacaagg ccgtaaaccc taaacgccgg taacactatt
     4921 ctcatgtttt acatgaaaga tatggctaac tgcctcagta tataggttta taaaaaaatc
     4981 accctccatg cagcattccg catcggatag aagtctattg aaaagataaa tgtattttcc
     5041 acggagcaaa aatgattttc tttttaagcc aaaggctaag cctgctactt acagggatac
     5101 atcgatttga agtaaaaatc tttctaaaaa gagtaaacca tgagaagatt tatgattacc
     5161 ggcacatttg ccctgatgac ttcaatgggc agctatgcgt acgcgcaggg acaagttaca
     5221 atccaaaacg acagcaagga ttttgtaggg gtaaagaata gcatctccgc tacagtaaac
     5281 ctctaccagg cttccacata caaggttgag ataacaacca gccagaaggt agccgacaag
     5341 ctggagattt cggtagaaaa cggtctgctg agcatcaggg agaaggacca atcatggaat
     5401 cattggagtt gcagcaacaa cacagatgac gaggtggtgg taaacgtttg gtctcctaaa
     5461 tattcaacgc ttgaaataaa cggttcgggc gatatgcttg caaaaacccc catcaaaaca
     5521 aacgaaattg aggtaaaggt taacggtagc ggcgatgtaa aaataaatga gctggaagcc
     5581 gaaaaagttt ccctcaagac aaacggctcg ggcgatattg ccgtaagtgg gaaaaaggag
     5641 gcatcaacgc tggtaatcaa aatcaacggt tcgggcgata ttgatacccg caacctggtt
     5701 accagggtag ccgacgtagc cattaccggt tcgggtgatg tttcggccaa cgtactggaa
     5761 aaatttaccg ccaactctgt tggtagcggc gatatttacc tctcaggaaa cccacaagtt
     5821 gatgcaaaga tattaggttc gggtgagata gtccgtaagt agcatcaagc aacaacacga
     5881 ataggtttag gtgaaaatag aggttttgct gaaatggcag ggcaaaggga gcaattccga
     5941 tgcctttcca tttttgaaaa aaaaactgca ccattgccta ttcgaatggt tcccagtttt
     6001 ttgccagtcc gccaattccg gtttccctgt aagccacctg aatatcacgg ccggtttcgg
     6061 ccatggtttc aactgcccgg tcaaacgaaa ttttatgccc tccatcagag gaaagggcgt
     6121 acactccgca ggagtatgcc ttggatgcag caatggcgtt acgctctatg cagggtattt
     6181 gcacgtagcc caacaccgga tcgcaggtta ggccaaggtt atgctccata cccatctcgg
     6241 cggcatactc aatctggttt ggagatcctc caagaacctg taccatagca gcggcagcca
     6301 ttgcgcaagc agttccaatt tctccctggc agcccacttc tgctcctgaa attgaagcat
     6361 tgtgcttaac caggttgcca attaatcccg cagtagccag cgccctatga atttttacct
     6421 cggtaaggta ctcttcccgg ctcaggtaga acagtatggc tggtaaaact cccgacgagc
     6481 cacaggttgg cgcagttaca attcttccac ctgcagcgtt ttcctcggca actgccaggg
     6541 caaaagaaaa cagtaggcca aagcttttta aagttccatg actggtaagt gctttcgaat
     6601 actgcgatga agccctcctg gcaagcttta tcggaccagg caaaaccccc tcgttttcaa
     6661 taccccgttt tatcgtttcc ctcatggtat gccaaactcc ctgcagaaat tcccatatat
     6721 cctttccctc gttatcctcc acaaactccc aaaaagtttt cccttccctc ctacaccagg
     6781 tcaaaatatc ggtcatgttg ttgagcgaat aaacctcact gctattcaac accccgcttt
     6841 tatcctttaa atcaccgccg ccaatgctgt aaacctccca ccggtcaagc gcctcggcac
     6901 cctcgtcggt caacgcctca aataccatcc cgttaggatg aagcggtagc gtttcctcag
     6961 gcttaaacac cacctccact gattttggaa gaaaacactt tcgtaaagcg ctatcggttt
     7021 ggtgacccct acccgttagc gctaaactcc caaatagcat tacccggaag tagctcacct
     7081 caggatgctt ctttatgaat agctctgcag ctcttaccgg cccaatggta tggctactcg
     7141 acgggccata cccctcctta taaacctcac gaattgatag catttgtctt aaaaatttag
     7201 gctgcaaagc tgtatcaaat tttttaaagg ggcaaacggc tgaaagtaat gcccccacaa
     7261 tttttactga aaagtataaa tgttgcctct ctgcgggcat gtcgtaataa atttttaatt
     7321 ttgagaatct aaacatgcca agttgcgcag taggtttttc ggcacttgat ccaaaacata
     7381 acgagcctgt ttgcccgata catacttatt gctattgcac cacggcatgt tgtccttcat
     7441 ccataaattg caagaatcat gaaacccatt atccaggcga cctcaatatc aaccgtaaca
     7501 tacatatcga atttactgat agggttaact atagcgctca cgcaattctt tacagctgaa
     7561 gcctattcgc aggataccct taaaacctac tgcaacccaa ttaatattga ttacggctat
     7621 agtccaatac cagcagcaat tgagcaggta aaacataggg ctacagcaga cccgctggtg
     7681 gtggcattca agggaaaatt ctttttattt tctaccaatc agcatggcta ctggtggagt
     7741 agcaacatgg ccagctggaa ctttattccc agaaaatttt tactcccccg acacaaggtt
     7801 tacgacgatc tctgtgctcc tgcggccttt gtgctgggcg attccctttt gctaatcggc
     7861 tcttcgaata accccgattt tcccatttgg gcatccacca accccgccgc cgacgattgg
     7921 catattgcaa ttgactcatt cccactagca gcatgggatc ccgccttctt tgcagatgac
     7981 gatggcagag tatatcttta ttttggctcc agcaaccttt acccaattta cggctgtgag
     8041 gttaacaggg caaccctgcg ccctcaagct gctccaaagc ccctgattag ccttaacgac
     8101 agtattcatg gatgggaacg ctttggcgag tacaacgaca ataccttttt acccccattc
     8161 atagaagggg catggatgaa caagcataac ggacattact accttcagta tgcggcgcct
     8221 ggaaccgaat ttagcgggta tgctgatggc gtttacattg gcagcaatcc tcttggacca
     8281 tttcgctatc aggagttcaa cccctttagc tacaaaccag gtggttttgc ccgtggggcc
     8341 ggacatggtg caactttttg cgattacaac cagcaatggt ggcatatctc aacaattgcc
     8401 attggagtaa aaaataattt tgaacgtaga ataggaatgt ggccggcata ctttgatgcc
     8461 gatggtacac tatactctca aactgctttc ggcgactatc cccactacct taatggaaaa
     8521 tttaccgaat ggatgctgct caactatcaa aaacccgtta ctgtttcaag caccctctac
     8581 ggttactatc ccaattttgc ggttgatgag gatattcgca catactggag cgcttctaca
     8641 ggcaacgctg gtgagtggat ttgtaccgat ttgctcgaca cctgccgcat atacgccttt
     8701 caggtaaact acgcagatca ggatgttcag cttgttggca agcaaatggg aatttaccac
     8761 cagtacagag tctggggcag cctcgatggt aagagctggt cgctactgat tgacaagtcg
     8821 gagaataaaa ccgatgttcc tcatgactat gttgagctaa gtacgccagt agaatttcgt
     8881 ttccttaagc tcgaaaacct ccatgtcccg gcagggaagt ttgccataag cggctttcgt
     8941 gtttttggaa aagccaacct gccacttcct gaatcagttg agttcttctc cgtacttagg
     9001 ggcaactccg aaccccgcaa tgcctggctg aaatggaaac ttcaggataa cgcaacggga
     9061 taccttatta attttggagt tcgtcccgac aaactatacg gaagcataat ggtttatggc
     9121 cataatgaat actattttag cggaatggat agggataaaa cgtactattt taccataacc
     9181 ccattcaatg agagtggttg tgggaacaga tcggctgttg ttaaatcgga aatcccttta
     9241 cgttaaacat tttcaaagca gctaggagtt aaactaaaat ttttgtaaag caaaacagta
     9301 ttagccacct gatgaattct aaaaaattgt ttggtagtag aatgaatagt tggtttaaga
     9361 gtctctcggc ttcaatagct aagcatagag tcggcatact caccaccata attattcacc
     9421 tgttggtgat tgcgtcattc ttggttttca agatagagga tagaacggag gaatatgcat
     9481 caaccattga aatggagttt gaggaaccca aggcgcagga ggaattgcta gttgaagaaa
     9541 agccagagca gctcctccca accgatgtaa ccgttcccag tcgtgaaatg gaggctataa
     9601 agaattttgc ggtagatgcc acccaaaagg agctaaacgc aggattgtcg gacgaaaaaa
     9661 acacggatgc cgacaacctg tacagcgaag ctaaccgcat aagggagcaa atgcaggaaa
     9721 acaaggagcg gtatgagcag gctatgaaag aagctgacat agccattccc aatacgcctg
     9781 aaaaaaatat accggaggag aaaaaaaata tgtacaaggg gccaacggta atctcgtata
     9841 acctggcagg ccggaaagca tacaggctac cagtcccctc ctataaatgc gaaatgggcg
     9901 ggcaggtggt tgtaaacatt gaggtgctgt ccgatggcag cgtttcaaat gccacaattg
     9961 actgggcgaa ctcggtgaat gacgagtgta ttaacaactc ggccattatg gccgcgttgg
    10021 ctagtcgttt ttcctcaacc caacaatcaa caggaaagga aaaagggagc attacgtacc
    10081 tatttgtggc ccagtagatt ggcaaaaccg tctagaatgc agccattagc aggttgatgt
    10141 cccgcgacgg caagttgtac agccgaccaa ggtaatcgtt ggtaaggatt ccgttaaaaa
    10201 cgtaaacccc atgccgcaaa cccgaatcct ccttcagctg gtgctggaaa cctccagcct
    10261 cgccaatgga tagcagcaac ggtgcaaaaa cattactcat ggcaattgag gctgttcggg
    10321 caacccgcga ggtaatgttt ggcacgcagt agtgaaccac gccatacttt tcgaacgagg
    10381 ggttgttgtg gtctcgaatc tcagatgttt caaaacaacc acccttatca atggccaggt
    10441 caacaataac ggatccatgc ttcatgttca tcaccatctc ctcgctaaca acgaagcgca
    10501 agttcgactc ctccggggta agagttccta tcaccacatc ggccgtcttc agcacccggg
    10561 ccagcacttg cggatggaat atggaggtgt aaagacgttg ccccagtaaa ttctgaagct
    10621 ccattagccg cttaatggag ttatcgaaca ccttgacagt agcgccaagc cccaatgccg
    10681 cacgagcagc aaactcggca gctgttccgg cgcccagtat tactacctca gtagggctaa
    10741 tcccggtgat accgccgagc ataacaccct tacccttatt tacattactt agatactcag
    10801 ctgccacaag caccgacgtg cttccagcaa tggagctcat cgcccgtata aaaggaaagg
    10861 agccgtcgga atcgcatatg ctctcaaagg cgatagcggt aacctttttt tgaattagct
    10921 tacggaggta gtcaccagtg gagctgttta gatggagcga cgacatgatt atctggttat
    10981 ccctaacaag ctcgaactcg gcgggaataa gcggagccac cttaagaact atatcgctgc
    11041 ggtaaacctc ctgtgaagag gtaacaatgc gcccaccctt ttcgctatag tctaagtcgt
    11101 tgtagtttgc ctgctttcct gcatccttct caatgatgat ttcgtgcccg ccctcaacca
    11161 acatctctac ggcttcaggg gttaaaggta tcctgctttc tgttgaatcg gcctctttgg
    11221 gaatccctat caccagctgc ttgtttttct ttacgaattc cagcctttcc tcctgaacta
    11281 aaagtccctt agcatgtgga aattcaggta aattcgatgc agtacgtttc atccgtacaa
    11341 aatttttggt ttctgattgc aattatctta ccccatacct tttaaccttc ggggttgaac
    11401 ttattgaaaa cccatattct ggtttcgccg aactcgtaag taacgctcgg tggttgaacc
    11461 gtatttgagg aagctccctt acatgggcat ttcatgatgg ttgttctaaa gctgagagga
    11521 tatcctaaaa gataaacatt cgtaagattt gctctcaatc ccgccagact tgggctatta
    11581 tatacgaatc tgcgtgaagt aaagttatag taatatcgat ttttacacaa aaaaaggtta
    11641 atcaaccatt atgtcaagtc tccgggaaaa gtcgggatta acggtaatga atagctttac
    11701 agtttggtcg gaaggtaaaa tatcctcaac cagctcgggc cattcgatga ggcagattcc
    11761 tgtggtggaa aaaaaatact cctcataccc aaaatcgtaa acctcctcaa tacggttgat
    11821 tcggtaaaag tcaaaatggt aaacaggctg cccgcttctg gtctgatact cgttaatcag
    11881 cgcaaatgtt gggcttgcaa caatgtcctt aacgccaagc gcatggcaga ttgcctttat
    11941 gagggtagtt ttccctgccc ccatttcgcc gtaaaaacaa acaacatggt attgcttaac
    12001 aatttcagcc acctcggagg ccacgctatc aagcgcctca attgtcggaa ttaacaagct
    12061 cttcattctg aaatgggttt cctttattgt tttgctatag ccagcgtggt agttgtattc
    12121 atagagcggg atatgaaata actgtacgaa atttacacgt atagcaagct agctggcagt
    12181 gcatagatta ctttggttta tagtgatgtt gtcgggtatg gatgaatcgt cggtcaattg
    12241 agttttttaa ccatctactt ccgctgcatt tctcgattgg taacatgggc tacctgtttg
    12301 gagtgagcac taccaccgga ataatcatct cctccaacga tattccccca tgctgaaagg
    12361 tgttacggta gtagctggca aagtagttgt agttgttggg atacacaaaa aaattacttc
    12421 cataggcaaa aataaaggag gaacttacgt taggctttgg cagatgagcg gtttcggggt
    12481 ctcgcacctc aaacacctcc ttggtattaa aggctaaact tctccccagc ttataccgga
    12541 ggttagttga agttgcccta tcgccaacca ccttaatggg gttttgaact ctaattgaac
    12601 catgatcggt ggtgattaca actttaatat cgaaacgggc agcttcgagc aggatattcc
    12661 tcaaaccgga gtgctggaac catgatcgaa ccagcgagat gtaggccgcc tcgtcggagg
    12721 ccagttcttt catcatgttg gagtcggtgc gtgcatgcga catcatatca acaaagttga
    12781 tcaccagcac ggtaaggttg ttgttcagca ccaggttaac attatccgct atgggcatgc
    12841 ccgcccgcag ggtgttacct ttttcgtagt gaaccttata gctttttccg agccgttgca
    12901 gctgggaggt gagcagctgg agctcatagt tgtttttccc ctcatcatca tcgtcggaaa
    12961 tccaaatatc gggaaacatt tgctgtatct ccagaggcat caaccccgca aaaatggcat
    13021 tccgggcata ctgggtagca gtgggcagga tgctgaagta aacatcatcc gactctaccc
    13081 tccactcgtc ggccagcagg ggttcaatag ccttccactg gtcgtagcga aggttatcaa
    13141 taagaaccag gaggaccttt tcgccggaat ctaataacgg aaaaaccttg ctgcgaagga
    13201 ggttgggaga taatagtggt ttatcgtcct ggttaccctt aaaccagcca atgtagctac
    13261 ttcgcacgta ccgagaaaat agctggttag cttcggccat ctgcatatca agcatctgcc
    13321 ttaggtttgg ctcactattg tcagaaattc ttagctgcca agcgctaagc ctacggtaga
    13381 gctcaaccca atcggagtag ctttgagcag cagcaatttg ctcgctcagc tggctaaatt
    13441 caaccccata atcggaagtt tgcttagcgc taaccagcgt tttaccatgc acgtgcttct
    13501 taatggaaag gagtatctgg ttgggattaa ctggtttaat caggtagtcg gcaatttgag
    13561 agccaaccgc catgtccatt atgttctcct cctcgctttt ggtaaccatt accaccggaa
    13621 ggtggggttt tagctcctta atttgtgtaa gtgtttcgag cccgcttagc ccgggcatat
    13681 tttcatcgag gaaaacaatg tcgaaaacat tgttacgaac cagctcaatg gcatccaccc
    13741 cattgatggc cttctgcaca tggtaacccc gctgttcgag aaagattata tgtgaaccca
    13801 ataggtctat ttcgtcatca acccaaagta tagatgctgg tgttgccata gcattttgtt
    13861 agttgtcaac actgtagttt tctttaaaaa atctggaata gtctgggata gacttatcgt
    13921 tcatgaaccg attaaagttc tcggtgctaa aaaggaaagc ttgaaattcg gcacggttaa
    13981 aatcggcaaa caccttttca tcctgcgtaa tcagcttgta gtagcttagg gcaatatcgg
    14041 cactctgaaa cccggtaaca cttaccacct cgacaaagtc gtttagcggt acgctgttta
    14101 aagccagatc catctcaata aaattatcca cattaaatgt tataatgttg aattttaact
    14161 ggttaatgtt ggtgcgttta ggaacaagca gaacaaaagc atgttctccc tcgggtttgg
    14221 tgtaaatgga tgtgcttatg ctgtcggagc taaccgaaac taccggcacg tcaatgctaa
    14281 cctttcctgt agaaagctgg aggtctctct gttcaaggta ggtcaacaaa ttctgggcaa
    14341 cctgcccctg ttcgctcttg gggaaagtca gggcaacttg ctcaagcgac tccttaaaag
    14401 ttctcaagtc gccagtcttt gactggcaga gagcatcaag tagcaagtat tttggctcca
    14461 tatcggtacc tttaaccttc cccatccccg attttagcgt tttggcagct tcgctgaaat
    14521 tgccctgaag gtaaagaccg taagccgttt cataaatgct gttggcttcg ttttctttca
    14581 ccgttagctc cttaatatac tcggggtttg aaagtattag cgcataaggg cttgaaggga
    14641 agcgggaaag tagcgcgatt ttgtagcctt cggcttcaac actattgccc tcatcagctg
    14701 caattctata catcctgtag atagccgtag ctgcttcgga agcatcgggg aaacgctcgg
    14761 atagcttttt gtacgattct ttggcggcct tgctgtcgtt gaggtctgtc tggtaaattt
    14821 ccgcaatcct catcatggcg gtaaggattt tctggttcga aatttgtttg agcgaatcgg
    14881 taaagggaag gttaaccagg tagaactctg gcgaggtgtt tgaaagttgc ttctgaggat
    14941 ttgctgcaga atcggatggt aagccgccag aggcaatgtt ttcgaagtca gaaacccctt
    15001 tattctttct gcgccagtta tcctctagct tacgcttgcc ccatttcatc tggaattccg
    15061 attgcccata gctaagcgag gcgggattat aaaagtacca tttcccgcct tgaggctcgg
    15121 agttgcttct gaatcgttgg ttctgcatgt agatttgcga ctgcatctga tcggcctgtt
    15181 cctgggcctt tagcttctgc tcctcctcct gaagtttacc aatgatggag gcaataagtg
    15241 catccctgtc ttttggagac atagcagcaa cccgttgaag gctgtcctcg gtgctaacaa
    15301 tgttcaggtt ttcaaccaga ttggttagaa accgggtttt ggtttccagc tgtgcgtatc
    15361 cggggaaact tgcatccaag gagttgaatg aactgtcgta gtaggcttgt gccttggcat
    15421 actccgattt ctgaaagtag tggtcggcca gcaccaggta cgacatgcct ttctggttat
    15481 cgttagtggt gctcaacctg gcagaaagca gatagtactc aatagcttta tccatgttgc
    15541 cttcctgctt ttccatgtta cccagcgtgt agtaaagctg atcgagataa tccttgttct
    15601 tttcatccga ggcaagcttc aggagctcac gtttcaagtc ttcacccttc atgttgctaa
    15661 gggcaatcag ttgaattcgt atgctaaatg ccatttcgta gggtggcccc atccgcatct
    15721 ccctgtcgta
//
LOCUS       DAOPHX010000053        15656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_4288_length_15656_cov_5.602718, whole genome shotgun sequence.
ACCESSION   DAOPHX010000053 DAOPHX010000000
VERSION     DAOPHX010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 15656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15656
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4288_length_15656_cov_5.602718"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PK502_05495"
     CDS             <1..209
                     /locus_tag="PK502_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00302.1"
                     /translation="PRGTTRPAYGVAQTVPLGHSRATFRPRPTCGSGFYAAFLIPATF
                     NDSRLYELRGEYLSPVGAEIKDKG"
     gene            complement(393..1181)
                     /gene="dapF"
                     /locus_tag="PK502_05500"
     CDS             complement(393..1181)
                     /gene="dapF"
                     /locus_tag="PK502_05500"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPH00303.1"
                     /translation="MLITFSKYHGTGNDFVMVDNRSGGLNPTVDLVEKLCNRHFGIGA
                     DGLILLENAKESALMMRYFNSDGRESTMCGNGGRCFAHFAHQLGLAGQHVLFNGIDGF
                     HEAYLLDNGNIRLRMIDVPVVESAEEGFFLNTGSPHFVVFVRSVENVNVDLEGRLASK
                     SVNVKNGGTNVNFIQPVGEKVLKIRTYERGVEAETLSCGTGSVASAIAYADFFAISEG
                     PVELLAPGGTLRVYFSHEGDGYKNIWLEGAVSHVFNGSIEVNEL"
     gene            complement(1395..2255)
                     /locus_tag="PK502_05505"
     CDS             complement(1395..2255)
                     /locus_tag="PK502_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPH00304.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGKEDLITVEEEVELAQRIR
                     KGDQAALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSKFEQEYERTPSPEELAE
                     LLDISKDKINDTLRISGRHVSVDAPFVDGEDNSLLDVIVNNDSPNADKGLINESLSKE
                     IERALATLTERERDIVRYFFGISCQEMTLEEIGEKFNLTRERVRQIKEKAIRRLRHTS
                     RSKLLKSYLG"
     gene            2672..3667
                     /gene="trxB"
                     /locus_tag="PK502_05510"
     CDS             2672..3667
                     /gene="trxB"
                     /locus_tag="PK502_05510"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPH00305.1"
                     /translation="MNLFTPLNSESGKNLSGSPKPVEHVRVLILGSGPAGYTAAIYAA
                     RANLSPVVYEGIEPGGQLTTTTEVENFPGYPDGVTGTQMMEDLKKQAERFGTDVRFGI
                     ATAADLSSMPYRITIDDEKVVETDALIIATGATAKYLGLESENKFKGQGVSACATCDG
                     FFYKGKDVAVVGGGDTAAEEATYLAGLCRKVYLIVRKSYLRASKAMQEKVANTPNIEI
                     LFEHTTKEIVGDQSGVNGVILTNTKTGKNVKVDISGFFLAIGHHPNSELFKPWLDTDE
                     VGYIKTLPDSTKTNLPGVFACGDVRDPHYRQAITAAGSGCMAAIDAERYLGERKF"
     gene            3963..4967
                     /gene="gap"
                     /locus_tag="PK502_05515"
     CDS             3963..4967
                     /gene="gap"
                     /locus_tag="PK502_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPH00306.1"
                     /translation="MGKIKVAINGFGRIGRNVFKLALERPELEIVGINDLTDTKTLAY
                     LLKYDSTQGRFEGKVEFDAENLIVNGKKYKISAERSPLNIKWMETPDVVVESTGIFTK
                     RESEKGGYGDHIKNGAKKVVLTVPAKDEIDRMVVVGVNEDTLKPTDQCISNASCTTNC
                     LAPVVKVLNDSFGIERGYMTTIHSYTNDQRILDAPHSDLRRARSAAVSQIPTTTGAAK
                     AVGKVIPELKGKLDGISVRVPTPTGSLVDFVAILKKEVTKEDINAAMKKAAEGPMKGV
                     LEYTEDPIVSADIIHNPHSSIFDAQSTMVNGNMVKVLSWYDNEWGYSARVVDLIPKLF
                     "
     gene            5209..5496
                     /locus_tag="PK502_05520"
     CDS             5209..5496
                     /locus_tag="PK502_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015661 - L-lysine efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903401 - L-lysine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HPH00307.1"
                     /translation="MITVVAIMAIGILVGYLLRQKRKLIGFLERLTTWAIYLLLFLLG
                     IAIGTNEEIIRNLPTLGGRAFIISVAGVLGSILLSWATYRLFFKKDEEKQE"
     gene            5493..6101
                     /locus_tag="PK502_05525"
     CDS             5493..6101
                     /locus_tag="PK502_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HPH00308.1"
                     /translation="MRGSLTILAFFAAGILLGFKDMLPQWLTATDLSSYALYLLMFLV
                     GIGIGGDDRALKAIKGFGLHIFLVPLTTIVGTLTGVAAVYLLLGQEPFTDMLAVGAGF
                     GYYSLSSIIISEIRGPELGVVALLANVMREIITLLMAPIFARYFGKLAPISSGGATSM
                     DTTLPIITASSGKEYAIVSLFHGIVLTILVPFLVTFILSLQS"
     gene            complement(6350..7597)
                     /locus_tag="PK502_05530"
     CDS             complement(6350..7597)
                     /locus_tag="PK502_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPH00309.1"
                     /translation="MKYAALINETFRISIRSITSTKLRSALTILIIALGIMSLVGIIT
                     AIKVIENSISTGFASMGANSFTIQSRGMHVNVNGNQYRTKNYSFISLQQAREFKDKFD
                     FPANVSVAVYATGASTIKYLSNKTNPNISVRGVDENFMMVSGLDVTQGRNYSVTEVND
                     GRNVAIIGNEVARKLFPKNEDPIDKFISVGSGKYRVIGVLKSKGAGFGGGPDRTVFLP
                     YTNVAAYFSRPNMNYSITVQPHDPKLMDAAVGEAEGIFRLVRRLSATDEPDFNIEKSD
                     TLAKLLIDNLKFVSIAATLIGIITLVGAAVGLMNIMLVAVAERTREIGTRKAIGAKSP
                     TIKQQFLFEAILICEIGGLLGVFLGILVGNLVSVLAKTSFVVPWGWIFGGLAISFLVG
                     LASGYIPAVRASRLDPIEALRYE"
     gene            7916..8962
                     /locus_tag="PK502_05535"
     CDS             7916..8962
                     /locus_tag="PK502_05535"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HPH00310.1"
                     /translation="MKIEERLNAFARLGDKFTKTLSCNEESELGAAIEKAHEQNPWFT
                     PNNVRFALATIAESWLKPDVLNSWIARYPEACFNPQHPVNVGVIMAGNIPLVGFHDFL
                     CVLATGNRITCKLSARDGNLMRSVANTLIQLEPRFSNLIAITDGTLRDFDAVIATGSN
                     NSARYFDYYFGKYPSIIRGHRNGIAVLTGKETQSNLTKLGEDIFTYFGLGCRSVSKLL
                     VPSGYSFTPLLNSFCQWKDLINHHRYANNYEYQRAIFTINQTPHFDTGFLLVTPSEQL
                     DSPVGVLYYQEYNKLDDALKYIGQHDKVLQCVVGLPEIYPKAIPFGSAQNPDIAEYAD
                     EIDTIVFLSQLQAL"
     gene            9020..9538
                     /locus_tag="PK502_05540"
     CDS             9020..9538
                     /locus_tag="PK502_05540"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00311.1"
                     /translation="MIYKFRMISSEDRAFLRDFEVDANQTFLTFHEAIQGNLNFDPSQ
                     LVSFFLADEHWNKGMELTLIDMQNEGDMAAIPMDSATVGELMKNRKERLLYVFDIYTD
                     RSLFIELLDIYEPTPGVTYPICTAAVGDPPLQFAGSDIENFSPELADDAGFEDFSNEL
                     DSDEAGSDSIDD"
     gene            9616..10545
                     /gene="miaA"
                     /locus_tag="PK502_05545"
     CDS             9616..10545
                     /gene="miaA"
                     /locus_tag="PK502_05545"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPH00312.1"
                     /translation="MSYNGNFLVILLGPTGVGKTDLSISIAKHFEAPIISADSRQFYQ
                     EMRIGTAAPTPSQLAGANHFFIGNKSITERYSCGMFEVDALNLLETVFTSSRIALLVG
                     GSGLYIDAVVNGIDDFPTPDPELRQALLSQLKNEGVASLRAQLKILDPEYYGKVDLRN
                     SQRVLKALEVCLQTGLTYTSFLTHRAKPRFFNSIFVGLNRPREELYRLINQRVESMMA
                     EGLLDEAKKLYPFRELNALNTVGYRELFSYLDGQTSLEEAIELIKRNTRRYAKRQLSW
                     WSRNKNITWFHPSQLTEITEFIEKTIASYSAKN"
     gene            10552..11268
                     /locus_tag="PK502_05550"
     CDS             10552..11268
                     /locus_tag="PK502_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPH00313.1"
                     /translation="MISNEKPNSEIFIELFTEIEQRLKTICNDEFTSNFSELLAKARG
                     KSGVVNRYASDLKEFAQLRNAIVHTRRQNFIIAEPHDDVVSEVIHIHKLLSSPPKVSS
                     VMKHNPFTVFPETPISVVLNTFAEKGFMRCPVVEQGRIVCLITAKTISRWITSIKQDR
                     IEVNNTPVRKLVPFADKSDYMVIAENDDIATLLDLFNESIRKGSYLQAALVTQSGEPQ
                     SKLVGIVTPSDLPLIMEKLK"
     gene            11422..13410
                     /locus_tag="PK502_05555"
     CDS             11422..13410
                     /locus_tag="PK502_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HPH00314.1"
                     /translation="MSDEPVEKIKGLPKNAYTELKDGEEYIPIMPPHKTVPEVNLWSV
                     TWGLVMAIIFSAAAAYLGLKIGQVFEAAIPIAIIAVGLSTMGKRKGALGENVIIQSIG
                     ASSGVIVAGAIFTLPALYILNLEAQFYQIFLSSLFGGILGILFLIPFRKYFVSDMHGK
                     LPFPEATATTEVLVSGEKGGKQARLLVVSGLIGGIYDFIIATFGWWSEVITSRVIPWG
                     EALADKSKLVFKLNVGAAVTGIGYIIGLKYTAIIAAGSFVGWFVIIPIINYFAPGLTA
                     AVGSNITAVVGSMSAEDIFTNYVRPIGIGGIAMAGIIGIINSSAIIRKALGLVVSELF
                     GKHKSMPKEELRTRRDLPMYIIAGGILLTTILILIFFHFGVVHNLAHALIGLAIVLII
                     SFLFTTVAANAIAIVGTNPVSGMTLMTLIITSLVMASVGLSGTSGMVAALIIGGVVCT
                     ALSMAGGFITDLKIGYWLGSSPFKQQTWKFLGTFVSAATVGGVIMLLNKTYGFTGKDA
                     LVAPQANAMAAVIEPMMSGKPAPWLLYAAGAFFSLILTMIKVPALPFSLGMFIPLQLN
                     TPLLVGGIISHIVSTRSKDAAVNNARKDRGTLVASGFIAGGALMGVISAILRFFNVNY
                     VNAQWFESHSAELLGLVMFLILCGYLIWESLRAKPEGE"
     gene            13608..15548
                     /gene="dnaG"
                     /locus_tag="PK502_05560"
     CDS             13608..15548
                     /gene="dnaG"
                     /locus_tag="PK502_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPH00315.1"
                     /translation="MIEQQVVDRIIAAADILEVLQDFVTLRKRGVNYLGLCPFHNEKT
                     PSFTVSPSKGIYKCFGCGKGGNVVNFIMEHEHLGYVDALKFLAKRYNIEIVEKEATPE
                     ELEQRNERESLMVVSAYAQKYFSNILHKHSDGKNIGMAYFRERGFHDNIIEKFQLGYS
                     LDQRNAFTRTALHDGYKLDYLVKTGLTIEGKDGNPFDRFAGRVMFPIHSLSGRVIGFG
                     GRVLRTDTKTAKYLNSPESEIYHKSEVLYGIFQAKKAITQEGKCFLVEGYTDVISLHQ
                     AGVENVVASSGTSLTHDQIKLIKRFTSNVTILYDGDAAGIKASLRGIDMVLEEGLNVK
                     IVLIPDGEDPDSFARSHSASEVLKFIDEHETDFIRFKTLLLLDDAKNDPIKRANLIGD
                     IVQSISVIPEVIKRSVYIKECSRLMDIEENVLYTEVNRQRRKRFEQLVQRESREPKEK
                     ETPRLPSYVSGVVCEEQEKEIISFLLNYGSKTLYNSQSEENKDDEINITVGQYIIDEI
                     LNDELEFQNLAYKMIFDEYAQLLSQNQAVDNRHFINHYNPQISQLAVDLMSSRHKLSR
                     IWEKHKAEVTDDPDFLLHAVPKAIQVYKSKVLKIAIKNLTDELTHLSAQQKDETDEIL
                     KRLLELNKLMNSMSKGLDRVVL"
BASE COUNT         4395 a         3733 c         3506 g         4022 t
ORIGIN      
        1 tgccacgcgg cacaactcgc cccgcctacg gcgtggctca aacagtgccg cttggacatt
       61 cccgcgctac gttccgcccc cgacccactt gcgggtcagg cttttatgcc gcttttctaa
      121 taccagcaac tttcaacgat tcccgccttt acgagctccg cggagaatac ctttcccccg
      181 ttggggctga aatcaaggat aaaggttaaa ggataaagga taaaaaaatt caagcagggt
      241 tcaagaactt gtccgactaa tggcggattc acccttcggc aaactcaggg caggctcatt
      301 cgacaggttt agtgcaaacc cgaaactctt tgctagttca ttaatttatt caaacctgca
      361 gcgaggttta ttttaggtct taacttatta gttcaaagtt cgttaacctc tatgcttccg
      421 ttgaatacat gactaacagc gccttcaagc caaatattct tgtagccatc gccctcatgg
      481 ctaaaatata ccctgagggt tccacctggg gcaagcagct caactggccc ttcggaaatc
      541 gcaaaaaaat cagcatatgc aattgctgaa gccactgagc cggttccgca tgaaagggtt
      601 tctgcctcca ctccccgctc atacgttctt atcttcaata ccttctcacc taccggctga
      661 ataaaattaa cgttggttcc accattcttc acatttaccg acttgctggc aagcctaccc
      721 tcaaggtcaa cgttaacgtt ctcaacacta cgtacaaaaa cgacaaagtg gggcgaaccg
      781 gtattgagga aaaagccttc ctcagcagac tcaacaaccg gtacatctat catacgtaac
      841 cgaatgtttc cattatcgag caggtaggct tcgtgaaaac catcaattcc gttgaagagc
      901 acatgctgcc cggctaaccc aagctggtgg gcgaaatggg caaaacaccg gccaccattc
      961 ccgcacatgg ttgactcacg cccatcggag ttgaagtagc gcatcatgag agcactctcc
     1021 ttggcgttct ccagaagaat tagcccatca gctccaattc caaaatgcct gttacaaagc
     1081 ttttcaacca agtcaactgt agggtttaac cctcctgagc ggttgtcaac cattacgaaa
     1141 tcgttgcccg ttccgtgata tttcgagaag gttatcagca tagcacgtgt gaatttttaa
     1201 aatttttggt gaaagttagg tgattttttc caataatgtt gacatcgggt taagacctct
     1261 tccccaatcg gtcatatccc aattagaatg caggagtaag agaacattca tcaacttcac
     1321 cccaccaaca ggtaacaaaa aaagccccgc acagggggct tttagttatc aaacaaatca
     1381 tccagcaaaa taggctaacc tagataagac ttcaacagct tactccgtga ggtatgacga
     1441 agtctccgta tggctttttc cttaatctga cgtacccgtt cacgggtaag gttaaacttc
     1501 tcgccaattt cctccagggt catctcctgg cagctgatgc caaaaaaata cctgacaata
     1561 tcacgttcgc gctcggtaag ggtggctagt gccctttcaa tttccttact caacgactcg
     1621 ttaattagcc ccttatcggc atttggagaa tcgttattaa caataacatc aagcaggctg
     1681 ttatcctcgc catctacaaa aggtgcatct acggaaacat ggcgaccact gatacgaagg
     1741 gtatcgttaa ttttatcctt tgagatatca agtagctcgg ccagctcctc aggtgaggga
     1801 gttctttcat actcctgttc aaacttagaa aaagccttgt taatcttatt taacgaaccc
     1861 acctggttta ggggcaggcg aacaattctg gactgttctg ccaaagcctg aagaattgac
     1921 tggcgaatcc accaaactgc ataggatata aacttgaatc cccgggtttc gtcaaatttc
     1981 tcggcggctt taattaaccc caaattcccc tcgtttataa ggtcgggcag gcttaacccc
     2041 tgattttgat actgcttggc tacagaaaca acaaatcgaa ggttggctcg ggttaatttc
     2101 tccagagctg cctggtcgcc cttcctgatt cgctgggcca actccacctc ttcctcaacc
     2161 gtaatgaggt cctccttccc aatttcctgt aagtacttat cgagcgatgc actctcccga
     2221 ttagtaattg attttgtgat ttttaactgt ctcattcgaa accttctatt tattttgcaa
     2281 agttatggct cttcaacatg caattcaagt ttatgcagca aaaaataatg actgcgaaac
     2341 tacttagctc aacttctata taatgcttcc ctaaaacggt tggcattttg aaattgttac
     2401 attattccgg ccgaatattc ttaattacta ttaaaaattt agcttgattc aattcaggct
     2461 taccgaaaat ggaggtgtat ttccaaaaca aaaaagcctt aagcaccttt attgctacaa
     2521 catttctcaa ggatatttgt tgtgagttgg ctgcgcctta atagctactg agtagcgata
     2581 tggccttttt tacactttaa aaacatgaaa ttagcagact aaaagcagta gatttgtaac
     2641 catcaatatt aaaatttcaa aagaataagc tatgaaccta ttcactcctt taaattccga
     2701 aagcggaaaa aatttatccg gatcaccaaa gccggttgag catgttaggg tgctaatact
     2761 aggctcgggg ccggcaggct atacagcagc aatatatgct gcacgagcca accttagccc
     2821 tgtggtttac gagggtatcg aacctggggg gcaactaacc accaccaccg aggttgaaaa
     2881 ttttcctggc tacccggatg gggttaccgg cacacagatg atggaagatt tgaaaaagca
     2941 ggccgaacgc tttggaaccg acgttcgatt cggaatagcc acagccgctg acctttcaag
     3001 tatgccctac cggataacca tagatgacga gaaagttgta gaaaccgatg cgcttatcat
     3061 agctacaggg gcaactgcca agtacctagg tctggaatcg gagaataagt tcaaggggca
     3121 aggcgtttcg gcatgcgcca catgcgacgg atttttctac aagggaaaag atgttgccgt
     3181 tgtgggtggt ggtgatactg cagccgaaga ggcaacttat cttgccggct tatgccgtaa
     3241 ggtttacctg attgttcgta aatcatacct gcgcgcatca aaggcaatgc aggaaaaagt
     3301 tgccaacact ccaaacatcg aaattctttt tgaacacacc accaaggaga ttgtaggcga
     3361 ccagtcgggg gtaaacgggg tgattcttac taataccaaa acaggaaaga atgtaaaggt
     3421 tgatatttcg ggatttttcc tggccattgg ccaccatccc aactccgaac tctttaagcc
     3481 atggcttgat actgacgagg tgggctacat caaaaccctg cccgattcaa ccaaaacaaa
     3541 tctgcctggt gtatttgcct gtggcgacgt tagggatccg cactaccgcc aagccattac
     3601 agctgccggc tcgggatgca tggcggctat tgatgccgaa cggtacctgg gtgagaggaa
     3661 attttagttt ggttttattc ggcataaaat ctaaaactgt ccttgtctgg gttaaaacac
     3721 cctctacaaa aactacctaa acaaagaaac catcaaatta ttaatatttt ttagcaattg
     3781 cggcaaaatt ttgaatttca tcgcaaacgg ttgaatacaa attggtagag catgctgtta
     3841 taagtggtat gccaattagg tgatttgaca agttgaaaaa aggggcggta aatgtaattt
     3901 tagcttatct ttgcttcacg aaaagggtta acatttacat aatcaactaa tacgaagtag
     3961 atatgggaaa aattaaagta gcaatcaacg gattcgggcg aattggccgt aacgtgttta
     4021 aactcgcact ggaacgtccc gaacttgaaa ttgttggtat taacgatttg accgacacca
     4081 aaaccctggc ctacctacta aagtacgact ctacacaagg gcgctttgaa ggcaaggttg
     4141 agtttgacgc cgagaacctg attgtcaacg ggaaaaagta taaaatatca gccgagcgct
     4201 ctccactaaa cattaagtgg atggaaacac ctgatgttgt tgttgaatca accgggattt
     4261 tcaccaagcg tgagagcgaa aagggtggat acggcgacca tattaaaaat ggcgccaaaa
     4321 aggttgtttt aacggtcccc gccaaggacg aaattgaccg tatggtggtg gttggcgtga
     4381 atgaggatac tcttaaacca accgaccagt gcatttccaa cgccagctgc accaccaact
     4441 gcctggctcc agtggtgaag gttttaaatg acagtttcgg aattgaacgg ggttacatga
     4501 ccaccattca ctcctacacc aacgaccaga ggattcttga tgcacctcac agcgaccttc
     4561 gcagagcccg ttcggcagcc gtttcccaaa ttccaacaac caccggtgct gccaaggctg
     4621 ttggcaaggt tatccctgag ttgaaaggta agcttgacgg aatatccgtt cgcgtcccaa
     4681 ccccaaccgg ttcgctggtt gactttgtgg ccattttgaa aaaagaggta accaaggagg
     4741 atattaacgc agccatgaag aaagctgccg aaggaccaat gaagggcgtt cttgagtaca
     4801 ccgaggaccc cattgtttca gctgatatta ttcataaccc ccactcctcc atctttgatg
     4861 ctcaaagcac catggtcaac ggcaacatgg taaaggtgct ttcttggtat gataacgaat
     4921 ggggctactc agccagagtt gtagatttga ttccaaaact attttagcca tacctagcga
     4981 taatgaaggg ctacccgcca ttgggtagcc ttttttaatt ttgtaaagta cacctatttg
     5041 attgcatttc atggttggag aataaattca acatctgaat tcataagcca aattcagact
     5101 aaaaaaagag tacttttcga ttttataccc cacccaatat tttacgcttg caagtgaatg
     5161 gtgcttcaaa atgtatagtc ggaaagtaga tttaaagttt catagcaaat gattacagtt
     5221 gtagcaataa tggccattgg gatactcgtt gggtatctcc tgcgtcagaa aagaaagctg
     5281 attggatttc tcgaaaggct tacaacgtgg gccatatacc tgctgctttt cttactagga
     5341 atagcaattg gaactaacga ggaaatcata agaaacctgc caacgctggg tggcagggcg
     5401 ttcataatca gcgttgcggg tgttctgggc agcattctac tttcgtgggc gacctaccgc
     5461 ctgttcttca aaaaggatga ggagaagcaa gaatgagggg tagtcttacc atactggctt
     5521 tttttgcagc aggcatactg cttggtttta aggacatgct tccccaatgg ttgactgcta
     5581 ccgacctgag cagctacgcg ctctaccttc tgatgttcct ggtgggaata ggaataggcg
     5641 gcgacgacag ggcgctcaaa gccatcaagg gattcggcct gcatattttt ttggttccgc
     5701 tcaccactat tgtgggaacg ctaacggggg tagccgctgt ttaccttctg ctgggacagg
     5761 aaccatttac cgacatgctt gccgttggtg ccggattcgg ctactatagc ctttcgagca
     5821 tcatcatcag cgagataagg ggtcccgagc tgggagtagt agccctgctg gccaacgtga
     5881 tgcgcgaaat cataacgctg ctcatggcgc ccatatttgc ccgatacttt ggcaagctag
     5941 cccccatatc atcaggcggg gcaaccagca tggataccac ccttccaatc attacggcct
     6001 catcgggcaa ggagtacgcc atcgtatccc tgttccacgg aattgttctc accattctgg
     6061 tcccctttct ggtaacgttt atcctttcgc tgcaaagctg aaattgacac caaccctaca
     6121 aataaggaag tatattttgg cttttgttgt ttcctggaaa tacctagcta ttaagggaac
     6181 aaatcaaaaa cagcaatgga attgaggcta aatgtttacg gattacagag attagggaga
     6241 tgcacgtagg ttctgtcagt acaaatttcc ctaataccct tcgtttttac cgtggttaaa
     6301 ttcccctcta ccataacata aagggaaata agcaaaaagc cagatgcccc tactcgtaac
     6361 ggagcgcttc gatgggatca aggcgcgaag ccctaaccgc aggaatgtaa cccgacgcta
     6421 aaccaaccaa aaaggaaatg gccagccctc caaaaatcca tccccaggga acaacaaagg
     6481 aggttttggc aagtaccgac accaggtttc ctaccagaat gcccaggaaa acaccaagca
     6541 acccaccaat ttcgcatatc agaatagcct caaaaaggaa ctgctgcttg atggtgggtg
     6601 attttgcccc tattgccttg cgcgtaccaa tctccctggt tctctcggca accgccacaa
     6661 gcataatgtt catcaaacca acggctgctc caaccagggt tattatccca ataagggttg
     6721 ctgcaatgga aacaaacttg aggttatcta tcagcaactt ggccagcgtg tcgctttttt
     6781 caatgttgaa atcgggctca tcggtggcgg aaagcctgcg aaccagccta aaaatacctt
     6841 cggcctcgcc aacagctgca tccatcagct tagggtcgtg gggctgaaca gttatgctgt
     6901 agttcatgtt gggacgtgaa aaatacgccg caacattggt gtagggcagg aaaactgttc
     6961 tatcgggacc accgccaaaa ccagcccctt tcgatttcag aacccctatc acccggtact
     7021 ttccgctgcc cacgctaatg aacttgtcaa ttggatcttc atttttggga aaaagcttac
     7081 gggctacctc gttaccaatt atggcaacat ttcttccatc gtttacctcg gtaaccgaat
     7141 agttccgacc ctgcgtaaca tccaggcccg acaccatcat aaagttttcg tcaacacccc
     7201 tgacgcttat gttggggttg gttttgttgg ataagtactt gattgtgcta gcccctgttg
     7261 cgtaaacagc tacgctgaca ttggctggaa aatcaaattt atccttgaac tccctcgcct
     7321 gttgaaggct gataaaggag tagttcttgg ttcgatactg gttaccgttt acgtttacat
     7381 gcatccccct gctctgaatg gtaaacgagt ttgcccccat gctggcaaat ccagtgctta
     7441 tgctgttctc aatcacctta atggcagtaa ttattccaac cagcgacatg atacccaacg
     7501 caattatcag tatggtaagc gcagaccgga gctttgtaga agtaatagac cggattgaaa
     7561 ttcgaaatgt ttcgttgatt agggcggcgt acttcacaac acttaaatta ggttaaacaa
     7621 atagtcagca cctcattttc tcatgaaaac ataaaacaac atcgtcttaa tgcgaagcta
     7681 taatttgatg ggtgtcccac atcaaatcct tttcttctaa agcattgctg tttttcatat
     7741 gctgcctgtt ccgttgaaaa actttcaaag ttattaattt ataccaattt tcgtccgaaa
     7801 agttaaggtc aatacaaaac ccttgcagca ggatgaagcc taccctgcac agctcaaaca
     7861 ttgcggtccg agccaaaata atactagctt tgcaacataa agacgcagtg ttaagatgaa
     7921 aatagaggaa agactaaacg cgtttgctcg tttgggtgat aaatttacca aaacgctatc
     7981 gtgcaatgaa gagtcggaac tgggtgctgc cattgaaaaa gcgcacgagc aaaatccatg
     8041 gtttaccccc aataacgttc gatttgcttt agctactatt gccgaaagct ggcttaaacc
     8101 cgatgtcctt aacagctgga tagcgcgcta ccctgaagcc tgcttcaacc cgcagcatcc
     8161 agttaacgtt ggtgtaatca tggccgggaa tatacctctt gttgggtttc atgattttct
     8221 ttgcgtgctg gcaaccggta accggattac ctgcaagttg tccgcaaggg atggcaacct
     8281 gatgaggtct gtggcaaata ccctgatcca gcttgagccg cgcttttcaa accttatagc
     8341 catcaccgac ggcacactaa gggattttga tgcagtaatt gctacgggta gcaacaactc
     8401 cgcccgatat tttgattact actttgggaa gtacccatct attatcaggg gccaccgcaa
     8461 cggaatagcc gtgctaacag gaaaggaaac ccaaagcaat ttaacaaagc taggagagga
     8521 tattttcacc tactttggcc tgggctgccg aagcgtttcc aagctattgg ttccatcggg
     8581 gtatagcttt actcccctgc tcaacagctt ttgccagtgg aaagacctaa tcaaccacca
     8641 ccgctatgcc aataactacg agtaccagcg tgcaattttc accataaacc aaacgcccca
     8701 ttttgacacc gggtttctgc tggtaacccc ttcagagcaa ctcgactctc cggtgggggt
     8761 actttactac caggagtata ataaacttga tgatgctctg aagtacattg gacagcacga
     8821 taaagtattg caatgcgttg taggattacc cgagatttac ccaaaagcca ttcccttcgg
     8881 atcggctcaa aacccagata ttgcggaata tgcagacgag atcgacacaa ttgtatttct
     8941 ttcacaactg caggctcttt aactttgaat atttgcattt attcactaaa actgcttatt
     9001 ttgtagcaaa gtataaagta tgatatacaa attcagaatg atatcgtcgg aggacagggc
     9061 attccttcgg gattttgagg ttgatgccaa tcaaaccttc ctaacctttc atgaggcaat
     9121 tcagggaaac ttgaattttg acccatcgca gctggtatca ttctttcttg ccgatgaaca
     9181 ctggaataaa ggcatggaac ttaccctaat agacatgcag aatgagggtg atatggctgc
     9241 tattcccatg gattctgcaa ccgttggcga gctgatgaag aatcgcaagg aacgcctgct
     9301 ttacgttttc gacatataca ccgatcgtag cctatttatc gagctgctcg acatttacga
     9361 gccaactccc ggagttacct accctatctg cactgcagca gtgggcgatc ctccacttca
     9421 atttgcaggt agcgacatcg aaaatttcag ccccgagttg gccgatgatg ctggattcga
     9481 agatttttcc aatgaattag attccgacga ggctggtagc gactccattg acgattaacc
     9541 cgcataaact cggtataaaa gcttcataaa gcatagctga aacgagggcg gttttagccg
     9601 cttccatgaa aaataatgag ctataacggc aattttttgg taatcctgct tggccccaca
     9661 ggtgttggga aaacagacct gagcatttcc attgccaagc actttgaagc gcccattata
     9721 tcagctgatt cacgtcagtt ttaccaggaa atgaggattg gaactgctgc acccaccccc
     9781 agccagctgg ccggagctaa ccactttttt attggcaaca agtctattac cgagcggtat
     9841 agctgtggaa tgttcgaagt tgatgcgctc aacctacttg aaaccgtttt tacatcaagc
     9901 agaattgccc tactggtagg aggttcgggg ctttacatcg atgctgtagt taacggaatt
     9961 gacgattttc ccactcctga ccccgagcta cggcaggcct tactcagcca gctaaaaaat
    10021 gaaggggtag ccagcctgcg agcgcagcta aaaattttgg atccggagta ctatggaaag
    10081 gttgacctga ggaattccca gcgcgtgcta aaagccttgg aggtttgcct gcaaactggt
    10141 ctcacctaca cctcattcct tacccatcgg gcaaagccca gattcttcaa ttcaattttt
    10201 gtggggctta accgtccacg ggaggaactc taccggctaa taaaccaaag ggttgaatcc
    10261 atgatggctg aggggttgtt ggacgaagca aaaaaacttt acccttttag agaactcaat
    10321 gcccttaaca ccgttggcta ccgcgagcta ttcagctacc tcgacgggca aacaagcctc
    10381 gaggaggcta ttgaacttat caaacgaaat acccgacgct acgccaagcg tcagctaagc
    10441 tggtggagcc gaaataaaaa tattacctgg ttccatccca gccagctgac tgaaattacg
    10501 gaattcatcg aaaaaaccat tgccagctat tcagcaaaaa attaaaaaaa tatgatctcg
    10561 aacgaaaaac caaactccga aatttttatc gagctgttca ccgagattga gcaaaggctc
    10621 aaaacgattt gcaacgacga gttcaccagc aacttctcgg agctgttagc gaaagcacgt
    10681 ggcaagagtg gtgtggtaaa ccgttatgca tctgatttaa aagagtttgc ccagctacgc
    10741 aatgccattg ttcacacccg ccgccaaaac ttcataatag ccgaacccca cgatgatgtg
    10801 gtgagcgagg ttatccatat ccataagctg ctaagtagcc ccccaaaagt atcgtcggtt
    10861 atgaagcata atccatttac cgttttccct gaaacaccaa ttagcgttgt tctaaacacc
    10921 tttgctgaaa aaggattcat gcgctgccct gtagtggagc agggtagaat tgtttgcctg
    10981 atcaccgcta aaaccatttc gcgttggatc acctccatta agcaggatag gattgaagtg
    11041 aataacaccc cggttagaaa gctcgtcccg tttgccgaca aatcagacta tatggttata
    11101 gctgaaaatg acgacattgc tactcttctc gacctcttca acgaatccat caggaaggga
    11161 agctaccttc aggcagcgct ggtaacccaa agcggcgaac ctcaaagtaa gctcgttggg
    11221 attgttacgc ctagcgacct accacttatc atggaaaaat taaagtagaa ccgggcagta
    11281 gagtttcgag cgctttgatg aggagtttcc tcagaatcac gctcgtaata agaagttgaa
    11341 tcactgaaaa gatttacttt ttttcttcta tttttgccaa ctagctaatc atcctaccta
    11401 aaatcttaaa aataaaaaaa catgagcgac gaaccggttg aaaaaattaa agggctccct
    11461 aagaatgcct acaccgagct aaaggatggc gaggagtaca ttccaataat gccgccccat
    11521 aaaactgttc ccgaggttaa cctctggagc gtaacatggg gactggtaat ggccataata
    11581 ttctcggcag cagcggccta ccttggacta aaaatcgggc aggtttttga agcggccatt
    11641 cccattgcca taattgccgt aggcctgtca accatgggaa aacgaaaggg tgctcttggt
    11701 gaaaacgtca tcatacaatc cattggagcc agctcggggg taattgttgc aggtgccata
    11761 ttcacccttc cggcgcttta catcctaaac ctcgaggcgc aattttacca gattttccta
    11821 tcgtcgcttt tcggaggaat tcttggcatc cttttcctga taccgtttcg aaagtacttt
    11881 gtatccgaca tgcacggaaa acttccgttt ccggaggcaa cagccactac cgaggtgctg
    11941 gtatcgggcg aaaagggagg caagcaggcg cgcttgcttg tggttagcgg tctaataggt
    12001 ggtatatacg attttataat tgccacattc ggttggtgga gcgaggtgat tacctccagg
    12061 gtaatcccat ggggcgaagc cttggccgac aagtcaaaac tcgtatttaa gttgaatgta
    12121 ggtgctgccg ttacggggat tggatacatc atcggactga agtacactgc tatcattgca
    12181 gcaggctcgt tcgttggttg gtttgttatt atccccatca tcaactattt tgcccctggg
    12241 ttaaccgcag cagtaggaag caacataacc gcagtggtgg gaagcatgag cgccgaagat
    12301 atttttacca actacgttcg accaatagga ataggcggca ttgccatggc cggaatcatc
    12361 ggaatcatca actcaagcgc tatcatacgt aaagcccttg ggttggtggt ttcagaactc
    12421 tttggaaagc ataaatccat gcccaaagag gaactccgta cacgcagaga cctaccaatg
    12481 tacattattg caggaggcat cctgctaacc accattctca ttcttatttt cttccacttt
    12541 ggcgtggttc ataaccttgc tcacgccctg atcggtctgg ccatagttct tataatatcc
    12601 ttccttttca ccaccgtagc ggccaacgca atagccattg ttggaaccaa ccctgtttcg
    12661 gggatgaccc tcatgacgct catcatcacc tcgctggtaa tggcttcagt agggctttcg
    12721 ggaacgtcgg gaatggtagc cgcactcatt ataggaggcg tggtttgcac cgccctgtca
    12781 atggcaggag gtttcatcac cgatttgaaa atcggctact ggttgggttc atcacctttt
    12841 aaacagcaaa catggaagtt tcttggtact ttcgtatcgg ccgccacggt tggcggagtt
    12901 atcatgctgc tgaataaaac ttacggtttt acaggcaaag atgcgctggt ggccccacag
    12961 gctaacgcca tggctgccgt tatcgaacct atgatgagcg gtaaacctgc tccctggctg
    13021 ctctacgctg ccggagcatt cttctccctg atacttacca tgattaaggt tcccgccctc
    13081 cctttctccc ttggtatgtt catcccgcta cagcttaaca ccccattact ggtaggtgga
    13141 ataatttccc atattgtttc tacacgctca aaggatgctg ctgttaacaa cgctcgtaag
    13201 gacaggggca cgctggttgc ctctggattc atcgccggag gcgcattaat gggtgttatt
    13261 agcgccatcc tgagattttt taatgtgaac tatgtaaatg cccaatggtt cgaatcgcac
    13321 tcggcggaac tgctcgggct ggtaatgttc ctgatactct gcggctacct gatttgggag
    13381 agccttaggg ccaaacccga aggggagtag caactcattt ctatactctc gatagcggct
    13441 accctgccat cttgctggct gtgtatccgc tatctttttg ttatccagca tgttatatgg
    13501 ccaaagcaac caatatgcaa agctgctttt ttaacaattc tatcggtcaa aaagaaatga
    13561 aaaaaacatc aaagttccta cctttaccat agaaataaag cagaaagatg attgagcagc
    13621 aagtagttga caggataatt gccgcagcag acatactgga ggtcctacag gatttcgtta
    13681 cccttcgtaa acggggagtt aactacctcg ggctatgccc atttcataac gaaaaaacac
    13741 cttcgtttac cgtcagccca tcaaagggaa tctacaagtg ctttggttgc ggaaagggcg
    13801 gtaatgtggt taacttcatc atggagcatg agcatttggg ctacgtggat gcgctaaagt
    13861 tcctggccaa aaggtacaat atcgagattg ttgaaaagga ggctactccc gaagagctgg
    13921 aacagcgaaa cgagcgcgaa agccttatgg tggttagcgc ttatgcacaa aagtacttca
    13981 gcaacatttt acataagcat tccgatggca agaatatagg aatggcctac tttcgtgaac
    14041 ggggattcca cgacaatatt atagagaaat ttcagctggg ctatagcctc gaccagcgaa
    14101 atgcctttac cagaacagcc ctgcatgatg gttacaagct ggattacctg gtaaaaaccg
    14161 ggctgaccat agagggcaag gatggtaacc ctttcgacag gtttgccggt agggtgatgt
    14221 ttccaatcca cagtctgagc ggacgggtga ttggttttgg cggacgcgtt cttagaaccg
    14281 acactaaaac agctaagtac cttaatagcc ctgaaagcga aatataccat aaaagcgagg
    14341 tactctacgg aatctttcag gccaagaagg caataaccca ggagggtaag tgtttcctgg
    14401 tggaaggcta caccgatgtt atttcgctcc accaggcagg tgtggagaat gtagtggcca
    14461 gttcaggtac atcgctcacc catgaccaga ttaagctcat caagcgcttc accagtaatg
    14521 ttaccattct ctacgatgga gatgcagctg gtattaaggc ttcattaagg ggtatcgaca
    14581 tggtgctgga ggagggtcta aacgtaaaga ttgtgctgat ccccgacggc gaggaccccg
    14641 acagctttgc ccgttcgcat agcgcttccg aagtgctcaa gtttattgat gagcatgaga
    14701 ccgatttcat ccgctttaaa acactactcc tactcgatga tgctaaaaat gatccgataa
    14761 aaagggcaaa cctgattggc gatatagtgc agtccatcag cgtgataccc gaggttatca
    14821 agcgttcggt ttacattaag gaatgctccc ggctcatgga tatcgaggaa aatgttctct
    14881 acaccgaggt gaacaggcag cgccgcaaac gcttcgagca gctggttcaa cgcgaatcaa
    14941 gggaaccaaa ggaaaaggaa acaccacgtt taccttcata cgtatcaggt gttgtttgcg
    15001 aggagcagga gaaggaaatt atcagcttcc tgctgaacta tggcagcaaa acgctctaca
    15061 acagccaatc agaggaaaat aaggacgatg agattaacat tacagttggc cagtatatta
    15121 ttgacgagat actcaacgat gagctggagt ttcaaaacct ggcctacaag atgatttttg
    15181 acgagtatgc ccagctgctt tcccagaacc aggctgttga caaccgacac ttcatcaacc
    15241 actataaccc acagataagc cagctggcgg ttgacttgat gtcgagcagg cacaagttga
    15301 gccggatttg ggaaaaacat aaggccgaag ttacagacga tcccgatttc ctgcttcatg
    15361 ccgtgccaaa ggctattcag gtttacaaga gcaaggtgct taagattgcc attaaaaatt
    15421 taaccgatga gctgacccac ctatcagccc agcaaaaaga tgaaacagat gaaatactca
    15481 agcggctact cgagctaaac aagcttatga actccatgtc gaaggggtta gacagggtag
    15541 tgctttagaa acagcagaaa agtaaacagg aaacaaggat aaagagattt caaggtaaag
    15601 gattcctgat tctagaacct gtccggctac tgacggaact tgtccggcta ccggcg
//
LOCUS       DAOPHX010000054        15648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_4292_length_15648_cov_6.197589, whole genome shotgun sequence.
ACCESSION   DAOPHX010000054 DAOPHX010000000
VERSION     DAOPHX010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 15648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15648
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4292_length_15648_cov_6.197589"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PK502_05565"
     CDS             <1..269
                     /locus_tag="PK502_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00316.1"
                     /translation="DPFAGLAFMPFYLYQQRSTILVFMSYAGNTFPRGAKDKDKYCLA
                     TDGTYPSNNEFHPICLMAQITPANFTSKFCNNFEKLATFETSLS"
     gene            366..1601
                     /locus_tag="PK502_05570"
     CDS             366..1601
                     /locus_tag="PK502_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPH00317.1"
                     /translation="MIEFSRHILPNGLTLIVHQDFSTPIAAVNMLYKVGSKHEHAEKT
                     GFAHLFEHLMFGGSENIPVYDEPLTAASGENNAFTNNDITNYYITLPAENIETAFWLE
                     SDRMLSLAFSEQSLNVQKKVVVEEFNQHYLNQPYGDVWLLLRPLAYAVHPYSWPTIGK
                     SPEHIQNATLIDVKDFFYHHYAPNNAILTIAGPVEPESTVELVQKWFGEIERRALDDK
                     NIPVEPKQESPRFLEVSRNVPFNAIYKAYHICRRNHPDFAPTDLISDILSNGKSARLY
                     QHLVKEKQLFSNINAFVTGDIDEGLLVVTGQLFPSTSFRDADESITSELEELKIKPVS
                     DYELEKVKNRFESNFVFEETSILNKAINLSFYEQLGNAGYINQEVEKYRAVSTNRIME
                     VAREILSVNNCSTLYYKKN"
     gene            1616..2917
                     /locus_tag="PK502_05575"
     CDS             1616..2917
                     /locus_tag="PK502_05575"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPH00318.1"
                     /translation="MTLSKLNRKEPPERRAVRKIELPEPSISTLPNGAKLAVINAGTQ
                     DIVKMEVIFNGGTRYQKKILSSQAAVSLMCEGTTTRNSNQLAEQLDFLGSFLQHSFDR
                     DFASVSLFTTRKHFAASLEVVADILTNPTYPENELEIFRKKGKQSLTVELEKVETLAK
                     QGFFQCLFGPDHPYGSFATPDDFDKLSQPDVADFHKNFHNLSNSFIILAGRVDEHEVK
                     TVETLLGANSPGRRLGALNNSLPAAAQTPREKFTYKENAIQSAVRIGKQMVSRNHPDY
                     IGLMVLNTILGGYFGSRLMQNLREDKGFTYGISSILIPYRETSVFMIGTEVGREVTKP
                     AVEEIYNELDKLVRVPVPAEELELVKSQMTGDLLRTLDGPLAIADSIAELYQYNNMNL
                     NFLDQTIETINTITSNKLQSLAISYFNPDEMVKSVAGAQPF"
     gene            2976..4574
                     /locus_tag="PK502_05580"
     CDS             2976..4574
                     /locus_tag="PK502_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPH00319.1"
                     /translation="MGKVKSTSVKLLTAILLMTAGQAAAQMYDKPKEPTFDYMTINIS
                     TGNPTLFWTPPSPDPQYPNPEGYIIYKHFVDILGNDFYEAIDTVDSSTFSYVDLTSNG
                     NLERLFYRIASKGPTEPSRQTAEHGQIWLTSSYDSCNAKIDLTWMHYAGWRNIDINQN
                     LKLYMSHNPDPSTFELIAEIDKYNSVYSITNVGENLDYYFYMTSGRIDKPFTTFSNLH
                     HIHTKMAKHPDYMTIDSVISVANGNAVYYSIDPTTEIRRFKVIRWEQPDTNKSIFSAK
                     VIEEFTDPAKNTTTDSTDSWAARTRKFYYKVDAYNGCSDAVKVTNLCNTIIPHVKPSG
                     KKVLLSWDTLMVDTYRQPDRINDWVEYTVYRRAYTSSSDLNGPGELSVAAQGLTEPSF
                     EDDLSAFQGHDPLYTITFKYYVEAIERSTYSTFCRSREIATEILPGATLPTAIDPTDP
                     TSINGHPRNIFQPVISFDASYNLTIYDRWGGVVYHGNQGWDGRNSSGEFVKEGTYIYR
                     LEINTSNAGKVVKNGSVSVVFPKH"
     gene            4760..6994
                     /locus_tag="PK502_05585"
     CDS             4760..6994
                     /locus_tag="PK502_05585"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPH00320.1"
                     /translation="MSLQTEEEKLLIQSHFEDLLRNCPRCSSEEDIKLITKAFNLANE
                     AHKGVRRKSGEPYILHPIAVAKIVSQEIGLGAKAIACALLHDVVEDTDYTIEDIERIF
                     GKKIASIIDGLTKITEVFDANSTIQAENFRKILLTLADDIRVILIKLADRLHNMRTLE
                     SLPATKQMKIASETIYLYAPLAHRLGLYSIKTELEDLSLKYRFPQVYEEIQSKIELNE
                     KRRVQLINHFSLPIIKKLEENNIDFEISGRPKSVYSIWKKMQTKNVSFEEIYDLLAVR
                     IVFNPYPHIPEKTQCWHIYSLITDIYKPKPDRLRDWVSTPKANGYEALHCTVMGPNGQ
                     WVEVQIRSQRMDEIAERGFAAHWKYKDMDNASHENELDKWIKRVREMLENPQENALEF
                     LDEFKVNLFTSEIIIFTPKGETRAMPKGSTALDFAYEIHTEVGNKAIGAKVNHKLVPL
                     SYILSSGDQVEIITAASQKPSWELLKYVTTAKAKNAIKNALKTETKDRLEKGKKLLDD
                     RLVELGVQPSSKILKKVLTSYEVSSKDELYSKIGSGQVNLDNLAKILKKNTKNKWIKY
                     WQLQLGRKSHPQNEPATLQKIDTKKPFLLSENVDSENPPYVIARCCNPIPGDDVIGYI
                     SPDDVVIIHKATCAEAIRLMAQHGDRIVSAKWTTHKVLSFLARIEVKGMDRIGIINDL
                     TRVISSELMVNIRKFHIESHDGIFEGFIDLYVHDISHLNNLIFNLMKLKGMDNVKRVD
                     KFDD"
     gene            7089..7562
                     /locus_tag="PK502_05590"
     CDS             7089..7562
                     /locus_tag="PK502_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPH00321.1"
                     /translation="MGCTETKTVVKQILSDYLQRKGYRETPERYAILQEIYSTDSHFD
                     VETLYIHMKNNGYQVSRATIYNNIDLLCECGLVIRHQFGKKNSLYEKAYECKQHDHLI
                     CTRCGKVTEFCDPRIQEIINSAKRNSNFTISHHSLYIYGICAECRNEIEKVPITL"
     gene            7773..9041
                     /locus_tag="PK502_05595"
     CDS             7773..9041
                     /locus_tag="PK502_05595"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPH00322.1"
                     /translation="MTKVDVLLGLQWGDEGKGKVVDVLTPNYDAIARFQGGPNAGHSI
                     EFNSIKHVLHTIPSGIFHPSCINIIGNGVVLDPIVFAEEVEILTKMNVDLHKTLLISK
                     KAHLILPSHRELDAASEAAKGKGKIGSTLRGIGPTYMDKTGRNGLRVGDILSPDFTRN
                     YEFLKNKHKELLQQYTYQSNIEQHEDAWFKGVEVIKQFRLVDSEYDINEMLVAGKRVL
                     AEGAQGSLLDIDFGSYPFVTSSNTICAGACTGLGVSPHSIGEVYGIFKAYCTRVGSGP
                     FPTEQENDTGEKMRNLGVEFGATTGRPRRCGWLDMVALKYAIMLNGVTRLIMTKADVL
                     DSFDTIRVAVAYLVNGKQTTRLPYDINVPVEPVYKEFKGWNSDITGITNEQDLPVELA
                     EYIRFIEHETGVPIHIVSVGPNRDSTIVRA"
     gene            complement(9057..9725)
                     /locus_tag="PK502_05600"
     CDS             complement(9057..9725)
                     /locus_tag="PK502_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00323.1"
                     /translation="MKRSAFFILVHLAAIFSCRAQGKVETDSSATMSIVRYATDLRCL
                     DAENAELLVNVLYEFNGLPPINRIELELPEAKEKVFRQIFVEVSSNDKKFIFRRKIRK
                     PDADELSALDPCALASKYSNLKRPVTVEWEIRVSLRSPAGSYKWPSIATGNFERIDHF
                     SFRVAYGKELSADIQTNLTTDPQKDTINGNFYYLWEFNDVNPSRTELKGFHSHDPYVI
                     LNFR"
     gene            complement(9799..11199)
                     /locus_tag="PK502_05605"
     CDS             complement(9799..11199)
                     /locus_tag="PK502_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPH00324.1"
                     /translation="MEKIRETLRDSAVARWTALALVAFTMLTGYYLTDVMAPLKGLLE
                     EQLTWNSTEYGIFTSAYGWFNVFLLMLIFGGIILDKMGVRVTGLGAAAVMITGTAIKF
                     WAVSTHSLDGQVWNILWLFPMKAQVFMAALGYAVFGVGVEVAGITVSKIIVKWFKGKE
                     LALAMGLEMATARMGTALALATSVPIAKSLGHVSKPILVCLIMLCIGFISFFIFTFMD
                     RKLDASQAAYDKDNNVQNDEEAFKVRDIFKIITNRGWWYIAILCVLFYSAVFPFLKYA
                     SDLMVNKFGISENLAGTIPAMLPFGTILLTPLFGNLYDRKGKGATIMLIGALLLILVH
                     SFFSLPFLTHWIVAIILIIILGIGFSLVPSAMWPSVPKIIPERQLGTAYSLIFWVQNW
                     GLMGVPALIGWVLDKYCVTGERITDSGLTVHTYNYTIPMLIFTSLGILALVFAFLLKA
                     EDKKKGYGLELPNIAK"
     gene            complement(11366..11830)
                     /locus_tag="PK502_05610"
     CDS             complement(11366..11830)
                     /locus_tag="PK502_05610"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPH00325.1"
                     /translation="MKKLTCVLILLTLLGSGCKFVEKYTKKGKKAKAEAMQKAKEDSL
                     AKAKAIEFEKAALAREQAHQDSIRQVEEYENQHRFHVIIGSFKVPSNATSWEQEVRGM
                     GFNNTRILQAPNGFNLVSVGAFNTYSKAFNEIDRINSNRIENPMEMWVYEKQ"
     gene            complement(12048..12896)
                     /locus_tag="PK502_05615"
     CDS             complement(12048..12896)
                     /locus_tag="PK502_05615"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPH00326.1"
                     /translation="MVRGICKTPDTPLVSSVVVHRGEEPVLSGEQGVCNVFFAHCNLQ
                     CSYCQNHQISRNSITNREWKMSVNEVAEEICGLLNRGISMLGFVSPSHQVVQMLEILE
                     LVRREGYSPRIIYNSNGYDSVETLRQLEGIVDIYLPDFKYVNPELGLRYSGVADYPDV
                     AAKAIREMYRQKGSSLLVDNDGLAESGMIIRHLVLPGYADESIRLLKFLAEEFTPRIY
                     LSLMSQYYPPVGVELQSPLNRTVTMEEYMGVVEVAQGIGFRGWIQDLESFGSYQPDFS
                     RSNPFE"
     gene            13491..15398
                     /locus_tag="PK502_05620"
     CDS             13491..15398
                     /locus_tag="PK502_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPH00327.1"
                     /translation="MKTIINLFEESVTKYPDNACIWEKLTDKYESSSYSEVRKEVYRL
                     AAGFLSLGIGKNDKVALLSEGRKLWITSELAILYTGAVNVPLSVKLDGNDLLFRLVHS
                     ETSTIIVSGIQAPKITRIKKEIPNLKRIIYLDSLNQYSEGEMGIEEVFKLGDTFLAEH
                     PGRVEEVSSSVKPSDFANISYTSGTTADPKGIILSHNNYTANVEQALTLMDIPQSYRT
                     LLILPLDHCFAHVAGVYAFMAMGASAATVQVGKSAIDTLKNIPINIKEIQPSLLLSVP
                     SLAKNFKKNIEVGIREKGKFTEKLFNNALKVSYRYNKEGWNKGNNFTFIYKPLIWIYD
                     KLIFSKVRKVFGGRLEFFIGGGALLDMELQRFFYAIGIPMMQGYGLSEATPIISSNSL
                     KRHKLGSSGYLVKPLDLKICDESGNELPVGQKGEIVVRGENVMVGYWKNEKATADTIR
                     NGWLHTGDMGYMDKDGFLYVLGRFKSLLISSDGEKYSPEGIEGALEEHSKFIENVILY
                     NNQNPYTIALIVPSKESIRRALNEKGIDMNTHEAGIEAIKLINNEVSRFKTGGDLQNL
                     FPERWLPSAFSILEEPFTEDNGLINSTMKVVRGKVEEKYRDKIEHLYTVEGKNIYNNS
                     NISSMLKIISN"
BASE COUNT         4708 a         3751 c         3260 g         3929 t
ORIGIN      
        1 ccgacccatt tgcgggtctg gcttttatgc cgttttactt ataccagcaa cgttctacga
       61 ttctcgtctt tatgagctac gcggggaata cctttccccg tggggctaaa gacaaggata
      121 aatattgcct ggctaccgat ggaacttacc catctaataa cgaatttcac cccatttgcc
      181 ttatggctca aatcacccca gcaaacttta cgtcaaagtt ctgcaacaac tttgaaaaat
      241 tggctacttt tgaaacctct ttatcctaag gcataaaact ttagcagcta tcgcaggcaa
      301 catcctagcc atctgcctct cgaggttgcc cttttgctga cattagaagt taacaaccaa
      361 ctattatgat agagttttca cgacatattt taccgaacgg gttaacgctg attgttcatc
      421 aggatttcag caccccgata gctgcagtaa acatgctcta caaagtaggt tcaaagcatg
      481 aacatgccga aaaaacaggc tttgcacatc ttttcgagca tttgatgttt ggcggttcgg
      541 aaaacattcc cgtttacgac gaaccgctaa ccgcagcaag cggagagaac aacgccttca
      601 ccaacaacga catcaccaac tactacataa ccctgccagc tgaaaatatt gaaacagcat
      661 tctggctcga aagtgaccgt atgctatcac ttgccttttc ggagcaaagc ctaaacgttc
      721 agaaaaaggt tgtagttgag gagttcaacc agcactacct taaccagcca tatggagacg
      781 tttggttgct acttcgcccc ttggcttacg cggtacatcc ctacagctgg cctacaattg
      841 gtaaatcgcc cgagcatatc caaaacgcaa cccttattga cgttaaagat tttttctacc
      901 accactacgc cccgaacaat gccattctta caattgccgg accagttgaa cccgaatcta
      961 ctgttgaact cgtccagaaa tggtttgggg aaattgaaag gcgagccctg gatgacaaga
     1021 atattccagt tgaacctaag caagagtcac cccgttttct ggaagtaagc cgcaatgtcc
     1081 ccttcaacgc tatctacaaa gcttaccata tctgccgacg aaaccatcct gattttgcac
     1141 ctaccgacct gatatcggat attctttcaa atggcaagtc ggctcggctc taccagcacc
     1201 tggtaaagga aaagcaactc ttcagcaaca ttaatgcctt tgttaccggc gatattgacg
     1261 aggggctgct ggttgttacc gggcaactct ttccctctac atcttttagg gatgcagatg
     1321 aaagcataac ctccgagctg gaagaactca aaatcaagcc tgtttctgac tatgagcttg
     1381 aaaaggttaa gaaccgcttt gaatccaact tcgtttttga agaaaccagt attctgaaca
     1441 aggccattaa cctctcgttc tacgagcaac ttggcaatgc cggctatata aatcaggagg
     1501 tagaaaaata tagagcggtt agcacaaacc ggattatgga ggttgcgcgg gagatacttt
     1561 ccgtaaataa ttgctccacc ctatactata agaagaacta aacatcaaaa tataaatgac
     1621 cctcagcaag ctaaacagga aagagcctcc agaaagaaga gctgttagga aaatagaact
     1681 acctgaaccg tccatcagca ccctgcccaa cggtgctaaa ctggcggtaa taaatgcggg
     1741 tacccaggat attgtcaaaa tggaagttat ttttaatggt ggcacccgat accagaaaaa
     1801 aattctatcg tcacaggcag ccgtttcgtt gatgtgcgag gggacaacca cccgaaattc
     1861 aaaccagctt gcagaacagc tcgacttttt gggtagtttt ctgcaacatt catttgatcg
     1921 cgactttgcg tcggtatcgc tatttaccac ccgtaaacac tttgccgctt cactcgaggt
     1981 agtagctgac atcctaacca atcccacata cccggagaat gaactcgaaa tttttagaaa
     2041 gaagggaaaa cagagcctta ctgtggagct ggaaaaagtt gaaacactgg ctaagcaggg
     2101 tttctttcag tgtctttttg gtcccgacca tccctacggc agctttgcca ccccagacga
     2161 tttcgacaag ctatcccagc ctgatgtggc cgattttcat aagaacttcc acaacctttc
     2221 caacagcttc atcattctgg ccggaagagt tgatgagcat gaagtgaaga ccgttgaaac
     2281 acttctggga gccaattcac cgggaagacg tttaggggct ttgaacaatt ctttacccgc
     2341 agctgcacaa acccccagag aaaaattcac gtataaagaa aatgccattc agtcggcagt
     2401 aagaattgga aaacaaatgg ttagccgaaa tcatcccgat tacattgggc ttatggttct
     2461 aaacacgatt ctgggtggat actttggctc aaggcttatg cagaaccttc gagaggacaa
     2521 gggattcact tacggcattt catccattct gattccctac agggaaacat ccgtatttat
     2581 gattggcaca gaggtaggca gggaagttac caaacctgcc gttgaggaga tttacaatga
     2641 acttgacaag ctggttcgtg ttccggtacc cgctgaagag ctggaactgg taaaaagcca
     2701 gatgactgga gatctgcttc gaaccctgga tggcccacta gctattgccg acagcattgc
     2761 cgaactctac cagtacaaca acatgaattt aaatttcctt gaccaaacta ttgaaaccat
     2821 taacaccatc acctccaaca agctgcaaag cctggctatt agctacttca accctgacga
     2881 aatggtaaaa agtgtagctg gtgcgcagcc tttctgaaac caaaacttga atttaacagc
     2941 taattttcgg tttttaaccg ctaacaataa agtcaatggg taaggtaaaa tcaacgtcag
     3001 taaaactact tacagctatc ctgttgatga ctgctggaca agcagctgcg cagatgtacg
     3061 ataagcccaa ggaaccaacc ttcgactaca tgactataaa cataagcacg ggtaacccca
     3121 cacttttctg gactcctccc tcgcccgatc cccagtatcc taatcctgag ggctacatta
     3181 tctacaagca ttttgttgac attcttggaa acgatttcta tgaggcaatt gacactgttg
     3241 atagcagcac attctcctac gtcgatttaa cctccaacgg caatcttgaa agactattct
     3301 accgcatagc ttcgaagggg cccactgagc caagcaggca aactgccgaa catggccaaa
     3361 tatggctgac gtccagctac gatagctgca acgcaaaaat tgacctgacc tggatgcatt
     3421 atgccggttg gagaaatatt gatatcaatc agaaccttaa gctctacatg agccataacc
     3481 cggacccttc tacctttgaa ctcatcgccg aaattgacaa gtacaactcc gtttacagca
     3541 ttaccaacgt gggcgaaaac ctcgactact acttttacat gacatcgggc aggatagaca
     3601 aacccttcac aaccttctca aacctgcacc acatacacac caaaatggcc aaacatcccg
     3661 actacatgac cattgactcg gtaatttcgg tggctaacgg gaatgccgtt tactactcca
     3721 ttgacccaac aactgaaatc agaagattta aggttatacg atgggagcaa cccgacacca
     3781 acaagagtat ttttagcgca aaggtgatag aggagttcac cgaccctgca aaaaatacaa
     3841 ccacagacag cactgacagc tgggcagcac gcacccggaa attctactac aaggttgatg
     3901 cctacaacgg atgcagcgat gcggttaagg ttaccaacct atgcaacacc atcatccccc
     3961 acgtaaagcc tagcgggaaa aaagtgctgc taagctggga taccctgatg gttgacacct
     4021 accgtcaacc cgacaggata aacgactggg ttgaatatac cgtttatcga agggcctaca
     4081 cctccagcag cgatttgaat gggccaggag agttgagcgt tgcagcccaa ggcctaaccg
     4141 aaccatcatt cgaggatgat ctatcggcct ttcagggaca cgaccccctc tataccatca
     4201 ccttcaagta ctacgttgag gccattgagc ggagcactta cagcactttc tgccggtcgc
     4261 gtgaaattgc caccgaaata ctccccggag ctacacttcc caccgccatt gacccaaccg
     4321 acccaacttc gataaacgga catccccgta acattttcca accggttatt agctttgatg
     4381 ccagctacaa ccttaccatt tacgaccgat ggggaggcgt tgtgtaccat ggcaaccagg
     4441 gctgggacgg tcgcaattcc agcggggagt tcgtgaagga gggaacctac atttaccggc
     4501 tggaaattaa caccagcaac gctgggaaag ttgtaaaaaa tggtagcgtt tcggtggtat
     4561 ttcctaaaca ttaagatgaa cttccccaat ggcaaaatgt tttacattaa aagagctgat
     4621 tttccttagg ggaaaaaatc tccttccaca gtactttcca cccgggaaaa agcggataag
     4681 ccttattttt aaatacacac ccatcaccac aacctgttaa aaaacattca tcaaaatttc
     4741 caaacaactc catgctacca tgagccttca aaccgaagag gaaaaactgc tcatccaatc
     4801 acacttcgag gatctcttac ggaactgccc gcgatgtagc tccgaagagg atataaagtt
     4861 gattaccaaa gcctttaatt tggccaacga agctcataag ggtgttcgca gaaagtcggg
     4921 cgaaccctac attctacacc ccattgcagt agccaaaata gtttcacagg aaattggact
     4981 tggagctaaa gccattgcct gcgcgttgct ccacgatgta gttgaggaca ccgactacac
     5041 catagaggat atcgagagaa ttttcgggaa aaaaattgcc tccatcatcg acgggctaac
     5101 aaagataacc gaggtttttg acgcaaactc taccatccag gctgaaaatt ttcgcaaaat
     5161 actccttacc ctggccgacg acatcagggt tatcctcatt aagctggccg ataggctcca
     5221 caacatgcga acgctcgagt cgctgccagc taccaagcag atgaagattg ccagcgaaac
     5281 catctacctt tacgccccgt tggcacaccg tcttgggctt tactccatta aaaccgagct
     5341 ggaggatctc agcctgaagt accgctttcc ccaggtgtac gaggaaatcc aaagcaaaat
     5401 agagctgaac gagaaacgca gggttcagct cattaaccac ttttcgctac ccatcattaa
     5461 gaagctggag gaaaacaaca ttgactttga gataagcggt cgtccaaaat cggtttactc
     5521 catttggaag aagatgcaga ccaaaaacgt ttccttcgaa gaaatatacg acctactggc
     5581 agtgcggatt gtttttaacc cctaccccca cattccggag aaaactcaat gctggcacat
     5641 ctactcatta attacagata tctataaacc caagcccgac cggcttcgcg attgggttag
     5701 cactcccaag gcgaacggct acgaggctct ccactgcacg gttatgggac ctaacggaca
     5761 gtgggtggaa gttcagatcc gctcccaacg aatggatgaa attgccgaac ggggatttgc
     5821 tgcccattgg aagtataagg acatggacaa tgccagccat gagaacgagc tcgataaatg
     5881 gattaagcgg gttagggaga tgcttgagaa tccccaggaa aacgccctcg agtttctcga
     5941 cgagtttaag gtcaaccttt ttacctctga aatcatcatt ttcaccccaa aaggcgaaac
     6001 ccgtgcaatg cccaaaggct ctactgccct cgattttgcc tacgaaattc ataccgaggt
     6061 tggcaataag gccattggtg ctaaggtgaa ccacaagctg gtgccgctaa gctacatcct
     6121 ttccagcggt gaccaggttg aaattattac cgctgcttca caaaaaccct cttgggaatt
     6181 actaaaatac gttacaactg ccaaggccaa aaatgccatt aaaaatgctc ttaaaacaga
     6241 aaccaaagac cgacttgaga aggggaagaa gctgctcgac gacaggctgg ttgagctagg
     6301 cgtccagcct tcctcaaaaa tattaaagaa ggtgctcacc agctacgagg tgtcatcaaa
     6361 agacgaactc tacagcaaaa tagggtccgg tcaggtcaac ctcgacaacc tcgctaaaat
     6421 cctgaagaaa aacaccaaga ataaatggat aaagtactgg cagctccagc tggggagaaa
     6481 gagccacccc caaaatgaac ctgccacgct acagaaaatc gataccaaaa agcctttcct
     6541 tctcagcgaa aacgttgact ccgagaaccc tccctatgtg attgcccgct gctgcaatcc
     6601 tatacccggt gatgatgtta ttggctacat ttcgcccgac gatgtggtaa tcatccacaa
     6661 ggccacttgt gccgaagcca tccgccttat ggcccagcat ggcgatagaa ttgtcagcgc
     6721 caaatggaca acccacaagg ttctctcatt tctcgcccgt attgaggtta aggggatgga
     6781 tagaataggc attatcaacg accttaccag ggtcatatcc agtgagctga tggtcaacat
     6841 ccgtaagttc cacatcgaaa gtcacgatgg gatttttgaa gggttcatag atctttatgt
     6901 ccatgacata tcccacctca acaacctcat attcaacctt atgaaactta aagggatgga
     6961 caacgtcaaa cgggttgata aatttgacga ctaaacaaaa atactaacca gctgttaacc
     7021 aacttacaaa tcaagctgct aataggataa atttattgtc tcattccgta taacttaaaa
     7081 agcacgccat gggctgcaca gaaaccaaaa ctgttgtaaa gcaaatactt tccgactacc
     7141 tacagcgcaa ggggtaccgt gaaacccccg agcggtacgc catactgcag gaaatatatt
     7201 ccaccgatag ccatttcgac gtggaaaccc tttacatcca catgaaaaac aacggatacc
     7261 aggtaagccg ggctaccatt tacaacaaca tcgacttgct atgcgaatgc gggttggtta
     7321 tcaggcatca gtttggaaag aaaaactccc tttacgaaaa ggcttacgag tgcaagcagc
     7381 acgatcacct aatctgtaca cgctgcggga aggttaccga gttttgcgac cccagaattc
     7441 aggaaataat caactcggca aaacgaaact ccaacttcac catctctcac cactcgcttt
     7501 acatatacgg catttgtgcc gaatgtcgta acgaaatcga aaaagttcca atcaccctgt
     7561 aacgctccct tgccgggtat gtgtcatcac ggcaagcctg ggctatggtt gtcttttcca
     7621 cattactaat tccaacagcc aaccccatta ccagaatcca ctccgtatta aaattggtgc
     7681 tgtttaaaac ttttacagcg ccatttacct aaattttgta actttaagag gttaaatttt
     7741 atgatcgatt ccataatctt ttcatataag ctatgacaaa agttgatgta ctgctagggt
     7801 tacagtgggg cgacgagggg aagggcaagg tagttgatgt gcttacccct aactatgatg
     7861 caatagcacg ctttcaggga ggcccaaatg ccggccattc aattgagttc aacagcatca
     7921 agcatgtgct gcacacaatt ccctcgggaa tattccaccc ttcatgcatt aacatcatag
     7981 ggaacggggt ggtgctcgac cccattgttt ttgccgagga ggttgaaatt ctaactaaaa
     8041 tgaatgttga cctccataaa accctgctaa tttcaaaaaa ggcccacctg atactcccct
     8101 cccaccgcga attagacgca gcttccgaag ccgctaaagg aaagggtaaa attggctcta
     8161 cccttagagg cattggcccc acatatatgg acaaaaccgg ccgcaatggc cttagagtag
     8221 gcgacattct ttcccctgat tttaccagaa actacgagtt tttaaaaaat aagcataagg
     8281 aacttctcca gcagtacaca taccagagca atatcgagca gcacgaggat gcttggttta
     8341 agggcgttga ggtgataaaa cagtttcggc tggttgactc ggagtatgat attaacgaaa
     8401 tgctggtcgc tggtaaacgg gtgcttgctg agggcgctca gggttcgctc ctcgatattg
     8461 attttggttc gtacccattc gttacctcct caaataccat ttgcgccggc gcatgcaccg
     8521 gtttaggagt ttcgccccat agcattggag aggtttatgg aatttttaag gcatactgca
     8581 cccgggttgg ctcagggcct ttccctaccg agcaggaaaa tgataccggg gaaaagatgc
     8641 gtaacctggg cgtagagttt ggtgccacaa ccggacgtcc tcgacgttgc ggctggctcg
     8701 atatggttgc cctcaagtat gccatcatgc tgaatggagt tacccggctc atcatgacta
     8761 aagctgatgt actcgattcg ttcgacacca tcagggttgc cgtagcttac ctagtcaacg
     8821 gtaagcaaac aacccggtta ccctacgata tcaacgttcc ggttgaacct gtttacaagg
     8881 aatttaaggg ctggaatagc gatatcacag gaataaccaa cgaacaagat ttacctgttg
     8941 agctggcaga gtatattcgt ttcatcgagc atgaaacagg agttcctata cacattgttt
     9001 cggtaggccc caacagggat tctaccattg ttagagcctg accaaccctt cttttgttag
     9061 cgaaaattta ggataacata cggatcgtgg ctgtgaaagc ctttcaactc cgtacgtgaa
     9121 gggttaacat cattaaactc ccagaggtag taaaaattcc catttatagt atccttctgg
     9181 ggatcagtgg taaggttggt ttgaatgtcg gcagaaagtt ccttgccgta tgctaccctg
     9241 aagctaaagt ggtctatcct ctcaaagtta cccgtagcaa ttgatggcca tttgtagctg
     9301 cctgctggtg agcgaaggct tactcttatt tcccactcca cggttacagg cctttttaaa
     9361 ttactgtact ttgaagccaa tgcgcagggg tcgagggcgg ataactcatc ggcatcgggc
     9421 ttgcgaattt ttcttcgaaa aataaacttc ttatcattac tgcttacctc tacgaaaatt
     9481 tggcgaaaca ccttctcttt agcctcgggc agctccagct caattctgtt tatggggggc
     9541 aggccgttga attcgtagag aacgttcacc agcagttcgg cattctctgc atctaaacat
     9601 cttaaatcgg tagcatagcg aactatgctc atggtagcgg agctgtcggt ttcaactttt
     9661 ccctgagccc gacaagaaaa aatcgctgcc aggtgaacaa gtataaaaaa tgcagatcgc
     9721 ttcattaaac ttttctaata tgcgttaaaa cgcccttgtt gggggcgttt caacgcatta
     9781 gtccattaca taagctgact acttagctat attagggagt tccagaccgt aacccttttt
     9841 cttgtcctct gctttaagca gaaaagcaaa caccaacgcc agaattccga gcgaagtaaa
     9901 tatcagcatg ggaatggtgt agttgtaagt atggacagta aggccactgt cagtaattct
     9961 ctctccggtt acacagtact tgtcgagcac ccagccaatt aaagcaggaa cgcccatcaa
    10021 cccccagttt tgaacccaaa aaatcaagga gtaagctgtg ccaagctgac gttcgggtat
    10081 aattttgggt acagatggcc acatggccga gggcaccagc gaaaatccta ttcccagaat
    10141 gattataagg atgatggcca ctatccagtg agtaaggaat ggtaatgaga aaaaggaatg
    10201 aaccaggata agaagtaggg cgccaatcag cataatagta gcacctttcc cctttcggtc
    10261 gtataagtta ccgaagagag gggtaagcag aattgttccg aatggaagca tggctggaat
    10321 ggtgccggca agattttcgc taattccaaa cttattgacc ataaggtccg acgcatattt
    10381 aaggaatggg aaaacagcgg agtagaaaag cacacaaaga atggcaatat accaccaccc
    10441 gcggttagta attattttga aaatatcacg aaccttaaat gcctcctcat cgttctgcac
    10501 gttgttatcc ttgtcgtagg ctgcctggga ggcatcaagt tttctatcca taaaggtgaa
    10561 aataaagaat gaaatgaagc ctatgcaaag catgattagg caaaccaaaa tgggttttga
    10621 gacgtgtcct agagattttg ctattggaac cgatgtggct agtgccagag cagttcccat
    10681 tcgggcagta gccatctcga gccccatggc aagtgcaagt tctttcccct taaaccattt
    10741 cacaattatt ttcgaaacgg ttattcctgc cacttcaacg ccaactccaa aaactgcata
    10801 tcccagcgca gccataaaaa cctgagcttt catcggaaac aaccagagta tattccatac
    10861 ctgcccatca agcgaatggg ttgatacagc ccaaaactta atagcagttc cggtaatcat
    10921 caccgctgct gctcccaggc cagtaaccct tacccccatt ttatccagaa taatgccacc
    10981 gaaaatcagc attagcaaaa aaacattaaa ccaaccgtaa gcactggtaa atattccata
    11041 ctccgtgctg ttccatgtaa gctgctcttc tagcaagccc ttaagcggag ccattacgtc
    11101 ggttaggtag taaccggtta gcattgtaaa agcaacaaga gccaaagccg tccatcgtgc
    11161 tactgcagag tcacgaagtg tctctctaat tttttccatt ctctataaat taatttaatt
    11221 attgattttt aagggttgaa aaaaatttca aacgaacgca aataaactta aatccaatct
    11281 aaaatcctat gagattgacc aatatttgaa aataaaaaac cgtcatgaaa atgatgacgg
    11341 ctaaagagtt tagggaatga agcagctact gtttttcgta aacccacatt tccataggat
    11401 tctctatcct gttggagttt atccggtcaa tttcgttaaa agccttgctg taggtattga
    11461 aggcaccaac cgatactaga ttaaagccgt ttggcgcttg gagtattcgg gtgttgttaa
    11521 accccatacc cctgacttcc tgctcccagc tagtggcgtt ggatggaacc ttgaagctgc
    11581 caataataac atgaaaccgg tgctggtttt catattcttc tacctgcctg atggaatcct
    11641 ggtgtgcctg ctcccttgcc agagcggcct tctcaaattc tattgccttt gccttggcaa
    11701 ggctatcttc ctttgctttc tgcatggctt cggctttagc tttcttgcct ttcttggtat
    11761 atttttcaac aaacttgcat ccgcttccta gcagggtcag caaaattagc acacaggtaa
    11821 gttttttcat ttggagtttg ggttagttgt atatttcaat agttgtgcaa gttagcaaaa
    11881 gttatgctaa aaatgcaagt gcaatccctt taaaatttcc ccgtccttcc aattttattc
    11941 ctaagggttc cgattcttca tgttaaaaaa gtgtgttaac acagcggaag ggtaaaccga
    12001 tagcccggct ggatgggttt cttccattta aaaaattata gctttaccta ctcaaaaggg
    12061 ttactccgtg aaaaatcggg ttggtagctt ccgaaactct cgaggtcctg aatccagccc
    12121 ctaaaaccga ttccctgagc aacctctact acccccatat attcctccat tgtaaccgtt
    12181 ctgtttagag gggactggag ctccactccc acggggggat agtactgcga catcagcgag
    12241 aggtaaatgc gcggcgtaaa ctcttcggca aggaatttga gtagcctaat gctctcatcg
    12301 gcatagccgg gaagcaccaa gtggcgaata atcatacccg attcggccaa accatcgttg
    12361 tcaaccagca gcgagctccc cttctgacgg tacatctcgc gaatggcttt tgccgcaaca
    12421 tccgggtagt ctgccactcc cgagtagcgc aagccaagtt cagggttaac atacttaaaa
    12481 tcggggaggt aaatatccac aattccctca agctgcctga gggtttcaac gctgtcatag
    12541 ccgtttgagt tgtaaataat tctgggagag tacccttccc tcctgactag ctccagaatc
    12601 tccaacatct gaaccacctg atgcgaaggc gaaacaaaac ccaacatgct aatgccacgg
    12661 ttaagcagcc cacaaatttc ctctgcaacc tcatttacac tcatcttcca ctcccgattc
    12721 gtaatggagt tccggctaat ctgatggttc tggcagtagc tgcactgaag gttgcagtgt
    12781 gcaaaaaata cgttgcaaac tccttgctca cccgaaagca ccggttcctc tcccctatgc
    12841 accacaaccg atgaaaccag aggagtatcg ggggttttac aaatacccct caccatacta
    12901 cgatttgccc cacagccgtg cgggcaaaga ttacaactta aaagattacc atggtcaatc
    12961 acctaaataa atttttgcaa atatacgcac ttcccatttt ttcttgtctc tcaactcacc
    13021 taacctggtt gcttacatta tgattgtttt gtgttcaaca tacaggctgt tccgctttgt
    13081 tgttattaca atacattttt gacccaacaa cccattgtaa taatttcaag gttgaagccc
    13141 tcagggcatc cttctcgtta atgcagcttt aaccaggcta attattgttg gctagtatca
    13201 aacaatttta tctatttgta agcaagataa ccctttcgtg tatccatttg taacacgaac
    13261 aatatacccc aaaaatacta cgccccttgg tacattctct aaatggtgta catttgccct
    13321 aggaatcact agttttacct gcctatctgt cagaaaacca gtagttttag cacaccaaaa
    13381 agaaaaataa atgtagatga acagaatttt ggatttaaac tactatgata aagaaaagga
    13441 tgtataattt tgtaacaagt taaatattaa cacattaaaa catagttcgg atgaaaacaa
    13501 tcatcaatct ttttgaggaa agtgtaacga aataccccga taacgcctgc atttgggaaa
    13561 agctaaccga taagtatgaa tcatcctcat attctgaggt aaggaaggag gtttaccggc
    13621 tggcagcagg ttttcttagc ctaggaatag ggaaaaacga taaggtagca ctgctcagtg
    13681 agggtagaaa gctgtggatc accagcgaac tggcgattct ttacactggt gcagtaaacg
    13741 tccccctgtc ggttaagctg gatggcaatg atctgctttt ccgtttggtt cactccgaaa
    13801 cctcaaccat tattgtttca ggaatacagg cacctaaaat aacaagaatt aagaaggaaa
    13861 tacccaactt aaaaaggata atctaccttg attcgttgaa tcaatactcc gaaggtgaaa
    13921 tgggaataga ggaggtattc aagcttggcg acacttttct agcagaacat cctggaagag
    13981 tagaggaggt ttcctcatca gtaaaacctt ccgacttcgc caacatcagc tacacctccg
    14041 gaactactgc cgaccccaaa ggcattatcc tatcccataa taactataca gcaaatgtgg
    14101 agcaggccct aaccttaatg gatattcctc aaagctacag aacactcctc atacttccgc
    14161 tcgaccactg ttttgcacat gttgcaggtg tttacgcctt tatggcaatg ggagctagcg
    14221 ctgcaactgt gcaggtaggt aaatcggcaa tagataccct caaaaatatt cccatcaata
    14281 tcaaggagat acaacccagc ttactgctaa gcgtgccctc gctagccaaa aatttcaaaa
    14341 aaaatattga agtcggtatt agggaaaaag ggaaatttac cgaaaagctg ttcaacaatg
    14401 cccttaaagt ctcataccgg tacaacaaag aagggtggaa taaaggaaat aattttacat
    14461 ttatttacaa gcctttaatc tggatatacg acaagttgat attcagcaaa gtaagaaagg
    14521 ttttcggggg ccgtttggaa ttttttattg gtggtggagc gttactagac atggagctcc
    14581 agcgcttttt ctatgcaatt ggtattccca tgatgcaggg atacggcctt tcagaagcaa
    14641 cgcccattat cagctccaac tccctaaaaa gacataagct tggttcatct ggatacctgg
    14701 ttaaacccct cgatcttaaa atatgtgatg aatccggcaa cgaacttcct gttggccaaa
    14761 agggtgaaat tgttgtccgt ggcgagaatg tcatggttgg ctactggaaa aatgaaaaag
    14821 ccactgccga tactatacga aatggttggc tccacacagg tgacatgggg tacatggata
    14881 aagatggttt cctctacgtt cttggccgtt ttaaaagcct cctgatttcg agcgatggag
    14941 aaaaatacag ccccgagggc attgaaggag cacttgaaga acactcaaag tttattgaaa
    15001 atgttatcct atataacaac caaaatcctt acactatagc gctgatagtg ccttcaaaag
    15061 aatctattcg gagagctcta aacgaaaaag ggattgatat gaacactcac gaagctggta
    15121 ttgaagctat caagctaatc aacaatgaag tgtcccgatt taaaaccgga ggcgaccttc
    15181 aaaacttatt tccggaacgc tggttgcctt cagcattttc gattcttgaa gaaccattta
    15241 cagaagataa tggtttaatt aacagcacaa tgaaggttgt tcggggtaag gttgaagaaa
    15301 agtataggga taaaatagag catttataca ctgtggaagg aaaaaatata tataacaact
    15361 caaatataag ctcaatgctt aaaattataa gcaactagtg ctatttatat attataacct
    15421 gagttagctt gataaataaa attagtcagt taaaatgaac atacacgtat aaaataaaac
    15481 tagaagatat ttaaacaacc aatcctaata atctaaagac acatataaga tttaaatcca
    15541 actaggagaa taaaaaaaga acgtatatac cggggaaaca aatcaaggtg ctataataaa
    15601 aaaaagcccc ggtcggggta acccggccgg ggcggaggtg gggcggcg
//
LOCUS       DAOPHX010000055        15486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_4352_length_15486_cov_4.876029, whole genome shotgun sequence.
ACCESSION   DAOPHX010000055 DAOPHX010000000
VERSION     DAOPHX010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 15486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15486
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4352_length_15486_cov_4.876029"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PK502_05625"
     CDS             <1..670
                     /locus_tag="PK502_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HPH00328.1"
                     /translation="MFLAELVVLFVVFMFTILLVQGTRKIPVQYAKRIVGNKQYGGVR
                     QYIPLKVNAAGVMPIIFAQAIMFIPMLFARFDSTAGIAAAFNYTGFLYNFTFAILIIL
                     FTYFYTAIIINPNQMAEDMKKNGGFIPGVKPGKKTVEFLDSIMSKITLPGSIFLAIIA
                     ILPAFAMILGVNNQFAHFFGGTSLLILVGVVLDTLQQIESHLLMRHYDGLMKSGRVKG
                     RAGY"
     gene            698..1474
                     /gene="map"
                     /locus_tag="PK502_05630"
     CDS             698..1474
                     /gene="map"
                     /locus_tag="PK502_05630"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPH00329.1"
                     /translation="MLAIKTEEEIELLRLNNDLVSKTLAEVAKIVRPGITTKELDHRA
                     EEFIRDNGAKPGFLGYDGFPATLCVSVNDVVVHGIPSNRVLHEGDIVSVDCGTYMHGF
                     YGDSAYTFEVGDVAPQVKRLLRVTRECLEHGVEAAVEGARIGDVSHAIQTHAESNGYS
                     VVRELVGHGLGRDMHESPEVPNYGNKGRGPKLPAGLVICIEPMINLGTRYVYQDSDGW
                     AVRTQDGKPSAHYELAVVVRNGKAETLSTFQYVEQVITKS"
     gene            1487..1705
                     /gene="infA"
                     /locus_tag="PK502_05635"
     CDS             1487..1705
                     /gene="infA"
                     /locus_tag="PK502_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPH00330.1"
                     /translation="MSKQPAIEKDGTIVEALSNAMFRVELDNGHVIIAHISGKMRMHY
                     IKILPGDKVRVEMSPYDLTKGRITFRYK"
     gene            1725..1841
                     /gene="ykgO"
                     /locus_tag="PK502_05640"
     CDS             1725..1841
                     /gene="ykgO"
                     /locus_tag="PK502_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HPH00331.1"
                     /translation="MKVRTSIKKRSDDCKIVRRKGRLYVINKKNPKFKQRQG"
     gene            1885..2262
                     /gene="rpsM"
                     /locus_tag="PK502_05645"
     CDS             1885..2262
                     /gene="rpsM"
                     /locus_tag="PK502_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPH00332.1"
                     /translation="MARIVGVDLPKNKRGEVALTYIYGIGRSSSRRILDKAGISYDKK
                     VSEWDDNELNAIRTILNEEYKVEGELRSMVQMNIKRLMDIGCYRGIRHRLGLPVRGQS
                     TKNNARTRKGRKKTVANKKKATK"
     gene            2278..2667
                     /gene="rpsK"
                     /locus_tag="PK502_05650"
     CDS             2278..2667
                     /gene="rpsK"
                     /locus_tag="PK502_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPH00333.1"
                     /translation="MANKRVSSKKKVVKVDPVGQAHIHSSFNNIIITLTNSTGEVISW
                     SSAGKMGFKGSKKNTPYAGQTAATDCAKVAYDMGLRKVKVFVKGPGAGRESAIRTINA
                     AGIEVTEIVDVTPLPHNGCRPPKRRRV"
     gene            2712..3320
                     /gene="rpsD"
                     /locus_tag="PK502_05655"
     CDS             2712..3320
                     /gene="rpsD"
                     /locus_tag="PK502_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPH00334.1"
                     /translation="MARYTGPSTKIARKFGEPILGPDKYFEKKNYPPGMHGLMKKRKK
                     QSEYGVQLQEKQKAKYTYGLLERQFLNTFKKAARSKGITGEILLQLLESRLDNVVYRL
                     GIAPTRAAARQLVGHKHVTVNGSVVNIPSYHVKAGDIIGVREKSKSLEIISDSLAGSH
                     NTRYSWLEWNKDEMYGKFLNVPERSEIPENIKEQLIVELYSK"
     gene            3337..4329
                     /locus_tag="PK502_05660"
     CDS             3337..4329
                     /locus_tag="PK502_05660"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPH00335.1"
                     /translation="MAILAFQKPDKVIMLESTGTTGTFEFRPLEPGYGITVGNALRRI
                     LLSSLEGYAITWIRIRGVDHEFSTIPGVMEDVTEIILNLKQIRFKRIVDGMDEEKVTI
                     TITGQNQFKAGYLSNFLSGFKVLNPELVICRMEPDVKIEVDVHIGKGRGYIPAEENKP
                     EDAEFGIIPIDSIHTPIRNVKYSVENYRVEQKTDYEKLVIEIDTDGSIHPKDALKEAA
                     KILIYHFMLFSDEKITLETEDKFETEEFDEEALHMRQMLKQKLVDLDLSVRALNCLKA
                     ADVETLGDLVRYNKNDLLKFRNFGKKSLTELEDLLDGLNLSFGMDISKYKLDKD"
     gene            4336..4869
                     /gene="rplQ"
                     /locus_tag="PK502_05665"
     CDS             4336..4869
                     /gene="rplQ"
                     /locus_tag="PK502_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPH00336.1"
                     /translation="MRHNRKVNHLSRTSAHRKAMLSNMASSLILHKRINTTVAKAKAL
                     RIYVEPLITKSKNDTTHSRRVVFSYLQNKYAVTELFREVSQKVADRPGGYTRILKTGT
                     RLGDNADMCIIELVDYNTNYVKESAGKTEAKRTRRGGSRAKKEETPVVESPKSATSEV
                     NSEIPSEGEISEEKISE"
     gene            4959..6251
                     /gene="eno"
                     /locus_tag="PK502_05670"
     CDS             4959..6251
                     /gene="eno"
                     /locus_tag="PK502_05670"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPH00337.1"
                     /translation="MGQIVSIHAREILDSRGNPTVEVDVLTESGYFGRAAVPSGASTG
                     VHEAVELRDGDKGRYLGKGVLKAVNNVNTTISEEIVGMHVLDQQEIDEAMIKLDGTPN
                     KGNLGANAILGVSLAVAQAAAQMTTQPFFRYVGGVNARTLPIPMMNILNGGSHADNSI
                     DFQEFMIMPVGAESFSEAIRMGAEVFHNLKNVLKKLGYSTNVGDEGGFAPNLKSNEEA
                     VKVILQAIENAGYRPGEDVFIALDPASSEYYLPEENVYHLHKSSGDKLTPAQMVGYWK
                     DWVTRYPIISIEDGMAEDDWSGWKLMTDTLGSKIQLVGDDLFVTNVERLQKGIDEGVA
                     NSILVKVNQIGTLTETINAVRLADTHAMTAIISHRSGETEDTAIAHLAVALNTGLIKT
                     GSASRSDRIAKYNQLLRIEEMLGPSAIYPGRNFKYIKK"
     gene            6333..8231
                     /locus_tag="PK502_05675"
     CDS             6333..8231
                     /locus_tag="PK502_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HPH00338.1"
                     /translation="MAYKSLHHFINKLEEQNELIRISAFVDPVLEIAEITDRMSKQPN
                     GGKALLFENTGTSFPVLTNALGSINRICLALGVSGLDDVGSDINKLFAELAGPKTNIW
                     EKLGTIPTLGKLSAWLPAQISSRGACQEVVHAEPNINLLPVMQCWPFDGGRFITLPMV
                     NTKDPFTGIRNVGMYRMQVFDPKHTGMHWHQHKVGARHFEAYKKLGKRMPVAVALGGD
                     PVYTYAATAPMPDNMDEYLLAGFLRKKKVKLVKCLTVDLEVPEDVDFVLEGFVDPTEE
                     LAWEGPFGDHTGFYSLADWYPRFHLTCITHRRDAVYPATLVGVPPMEDAYIAKATERI
                     FLQPLRITMLPELEDLALPVEGTAHNIALVKFRDYYPGQAYKIMNAIWGAGQMMFNKL
                     LVALPKDIDLTNADQILHSLVNFVDPERDLMFGKGPLDILDHATPTQAFGSKLFIDAT
                     NQTVVMPHLQISINDVKELLERETSRVKNFNLNLLNRGISILIISVDKQAVTNVKSYM
                     QQLFRCQELAGLRAMVAVDMGDAVTDLSMLTWFASSNIDPIRDVFILNLNDGYPARVG
                     VDGTRKAYPQDNFKREWPNVVAMSREVVDHVNKQWEKYGIGGMVESPSKQLLPLVAGE
                     GPVVKLNS"
     gene            8490..11780
                     /locus_tag="PK502_05680"
     CDS             8490..11780
                     /locus_tag="PK502_05680"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPH00339.1"
                     /translation="MLLFFIKGGGELFAQKTEVEVFDANNGLPQTYVSCLIQDSKGFL
                     WLGTHSGLCLYDGYQFTTFSNNPLDSLSLCNNFVKSIAEDKNGDIWVATLSGLSRYER
                     ATGKFISYFSEPTNPASLAGDKIYYVYVDREKNLWVHTPGTLNLYSYKSNDFTRFNYF
                     NDLFTQPVETDIYPIYEDLQGRIWVGTKDGLMLFDRERMLFKRYSSRISYPYSIYGNR
                     VQAIYEDSRGNFWVGTDKGLNLFSFKLQRFYPVGAGIDENDAVRFIFEDSRSTLLLGM
                     ESGVARLHSSTEKVEQLQNFYSKSSKILPIGISAILKDKSGITWVASRTGLIKINPFS
                     QRFKGYSRDNLGNPLFSNNIIASVFEDSSGKLWVGTWGRGLFIFNPKTGENLRYCKSS
                     SIYRLPNDFAHTVFQTRKGEILVGTRNGVFVYDQRSRQFSNFFASRHVSVGNIFVDNR
                     VYSIDEDSAGNLWFATAIGLHCLSGNRLHSYYSRRGDSIFLPSNLIFDVLAGKQGEVW
                     VATANGLSRISDDCSSVINITVMTDSAGQGQQPLGEVYCLHQLSNGAILAGTSNGLVS
                     VNLLKGSKNLYFENLIIPSISARAIEEDVKGNFWLSTSKGITCYNPSTGQLASYTSDD
                     GLLGNEFNLLSSFQSPSGTIYFGGMQGISYFHPDSVMLNSHIPPLSFTKISLASVSST
                     KNIHPFGIDEVRIPQGFSSLTIEFSALDFNHPEKNLYRYRLQGPGSSKWVELGNKHSI
                     TLTNFREGVFRLEVMGANSDCVWTTEPLSLRIIVVAPFWRSRIAYTVYALLLLFVLIA
                     YGSYRRRNSLRIKRLLAEREDVMAKLELQKEELENKNKNITDSIHYAKRIQDAMLPTV
                     DMFKSHIPNSFILFKPKDIVSGDFYWVKETDSKCFVAVVDCTGHGVPGAFMSIIGIEL
                     LRNITEVEAVEDTAEVLNRLSASVHETFSSVSYPNESGLKVKDGMDVTFCTFDKQYDI
                     MQFTGAFSDLYIIRDGNIIEVKGDRFSVGMADDAGRYMFSSSYIPLMPDDMIYLFTDG
                     YVDQFGGPEGKKFKFRRFRRLLLNIYRLPLETQQKILEESITEWMGGQEQVDDILVIG
                     IRTPTKAK"
     gene            12027..14333
                     /locus_tag="PK502_05685"
     CDS             12027..14333
                     /locus_tag="PK502_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPH00340.1"
                     /translation="MKRLAILLLFSILTPIQNFGQTVSLVLSGGGAKGCAHIGVIKAL
                     EENGIPIDNVSGTSIGAIVGGLYAMGYSPDEMVNIFKSRDFYNWSNGLIDESYKFNIN
                     DLSTTDAENLSVGFTFDRGGLKTKLTSSIVPPVGLDIAFEEMFAQGTAISRGNFDKLF
                     VPFRCNASDVVNKKQVYFREGNLGKAIRTSMTFPLYFTPVYIDSLLLFDGGIYNNFLW
                     KEALQEFHPNIIVGSKVASNSKLPSDEDPLLQVEAMIVGITDYSIPDSLGIVIDTRLE
                     NIDLLDFDKVDEIVSAGYNETLKVIPKLREQITRNVTPEELAMRRKNFRQNLPPLFID
                     NIRVGNLSDRQKTYLHKVIFNEYSIVTFDKFKKDYYRLVSDRTFKRLIPEFEFDYTSG
                     LFDVALNPTLNRSVDLGLGLSLSSDVGNEGFASASYKWLSRTSNTIYGNIYFGKLLSS
                     IRLDYIKTFPTKIPVSLVACTIASRYDYHSSNPIPFFEDVKPPYVLQDETFATVSLKV
                     SNTSATTTTLSVSGGEKVDHYYQAMDYYSFDVPDQTTFKFIKGSLCFDKYTLDSKQYA
                     TRGRHQQLVISGFSGREEHYPGTTAPQMTGVRKNHSFATFYLYNESYHRIWRRRFWLE
                     LLFESYYSTQGLFSNYFASILTSNQFSPTPHSNLIFIENYRSTQYAAVGVAPIFEFWK
                     NVHFRVGAYLYQPFRTLKQDDVGVANYSDYFAKRWLIGSASLVYRTPVGPIALTLGYY
                     PTANSNGDDIFFNLTFGYSIFNSRVFDN"
     gene            complement(14345..>15486)
                     /locus_tag="PK502_05690"
     CDS             complement(14345..>15486)
                     /locus_tag="PK502_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPH00341.1"
                     /translation="RTPLNNIMVISNMLSNTITEDKYRDLIDTIQASTNSLAGVVNGM
                     VEKSSTGIEPETDFAIPFNLSDTINNTIRLFATQSQTVNFNIKIDESIPKTLKGDPVK
                     LKQIFLNIIENVIKNKEGSKVNIEIIASRMGNAKNSLELFFEVKSNVPLLIHQNEGYS
                     QTVIADSTSSSLNNQFFIDLLNLTITNNLVQSNGGKLNIKLTSNDAVVSFTYFLNSLP
                     EIKKVAEPEVLKTTQRQHEEISTLTAQPSAEAKGDGKLSNANVLLVEDNLINQKIVIL
                     SLKKLVKNIDVAANGKEALDKFGTSQYDIILMDVQMPIMNGYVATRKIREIEASTNSH
                     TPIIAITANALLGDREESLAAGMDDYISKPFQIEVLIQKMKKFLE"
BASE COUNT         4342 a         3237 c         3608 g         4299 t
ORIGIN      
        1 tatgttcctt gccgagctgg ttgtgctttt tgttgtattc atgtttacaa tcttgcttgt
       61 tcagggaacc cgtaaaattc ctgttcagta tgccaaacga attgttggca acaagcagta
      121 cggtggcgtt agacaataca ttccccttaa ggtgaacgca gcaggcgtta tgcctataat
      181 ctttgcccag gccatcatgt tcattcccat gctgtttgcc cggtttgact ccactgcagg
      241 aattgctgct gcttttaact ataccggctt cctttacaac tttacctttg caattttaat
      301 catccttttc acctatttct acacggcaat tatcatcaat cccaaccaga tggctgagga
      361 tatgaaaaaa aatggtggat ttattccagg cgttaaacca ggaaagaaaa cagtggagtt
      421 tttggattcc atcatgtcca agattaccct tccgggttca attttcctgg ccataatagc
      481 cattcttcct gcatttgcga tgatacttgg ggtaaacaac cagtttgcac acttttttgg
      541 gggcacatcg ctgttaattc ttgtaggagt tgttctggat acactacagc aaattgaaag
      601 ccatttgctg atgcgacact acgacggact gatgaagtct ggtcgggtta agggaagggc
      661 aggttactag ttgatgcctg cagctaagtt ctttacaatg cttgcaatta aaacggaaga
      721 ggaaattgag ctgctaaggc ttaataacga tttggtttcc aaaacgctag ctgaagtagc
      781 caaaatagtt cggcctggca ttaccaccaa ggagttggac catagagcgg aagagtttat
      841 acgtgataat ggcgcaaagc ctggctttct cggctacgac gggtttccgg caactctttg
      901 tgtatccgtt aacgatgtgg tggttcatgg aattccttct aaccgggtgc tgcacgaggg
      961 agatattgtt tcggttgact gcggaaccta catgcatgga ttctatggcg attcggccta
     1021 tacatttgaa gttggtgatg tggctccaca ggttaaacgg ttgctcaggg ttacccgcga
     1081 gtgcctggag cacggtgtag aagcagcggt tgagggtgcc cgcataggcg atgtttcaca
     1141 tgcgattcaa acccatgccg agagtaatgg ctactccgtg gttcgggagc tggttggtca
     1201 tggccttggg cgcgacatgc acgaaagccc cgaggttccc aattatggca ataaggggcg
     1261 cggacctaaa cttccggcag gtttggttat atgtattgag ccaatgatta acctgggaac
     1321 aaggtatgtt taccaggata gtgatgggtg ggcagttcga acgcaggatg gaaaaccatc
     1381 tgcacactac gaacttgccg tagtagtgag gaatggaaaa gccgaaaccc tctccacttt
     1441 ccagtacgtt gaacaggtaa ttactaaaag ttaataaggc gagcggatgt cgaaacaacc
     1501 ggcaatagaa aaagatggaa ccatagttga agcattgtca aacgcaatgt ttcgggtgga
     1561 gcttgacaac ggccatgtta ttattgcgca tatatcgggt aaaatgcgca tgcactacat
     1621 caaaatacta cccggcgata aggttagagt ggagatgtcg ccctatgatt taaccaaagg
     1681 acgcataaca tttaggtata agtaaactta aattatcaaa ggagatgaaa gtaagaacct
     1741 caatcaagaa gcgcagcgac gattgtaaaa tagtcaggcg aaaaggccgc ttgtacgtga
     1801 ttaataaaaa aaatcctaag ttcaagcagc gtcaaggata aattgtttaa ttttgcagtc
     1861 taaaaatttt ttaagaaatc aggtatggca cgaatagttg gtgttgattt gccgaaaaat
     1921 aagcgtggtg aagttgcgct aacctacatt tacgggatag gccgaagcag ctcgcgcagg
     1981 attcttgata aggcgggaat tagctacgat aagaaggtgt ccgaatggga tgacaatgag
     2041 ctcaacgcca ttagaaccat tttgaacgaa gagtataaag tagaaggcga gcttcgctca
     2101 atggtgcaga tgaacattaa acgcttaatg gacattggtt gctaccgtgg aattcgccac
     2161 cgtcttggtt tgcctgttag aggccaatca accaagaata acgcccgcac tcgaaagggc
     2221 cgtaaaaaaa ctgttgccaa caagaagaaa gcaaccaaat aatattgtgt acaaactatg
     2281 gcaaacaaga gagtatcttc aaagaagaaa gttgttaagg ttgatccggt tggacaggca
     2341 cacatacatt cttcattcaa caatattatt attacgctga caaacagcac aggagaagta
     2401 atttcctggt catcggcagg gaaaatggga tttaagggct caaagaaaaa caccccctat
     2461 gccggccaaa ctgctgctac cgattgtgct aaggtagcct atgacatggg gctgcgtaaa
     2521 gttaaggtat ttgtaaaagg acccggagca ggtcgtgaat cagctatacg aactattaac
     2581 gctgctggca ttgaggtaac cgaaatcgta gatgttactc ctttacccca taacggctgc
     2641 cggcctccaa aacgccgcag ggtataattc ttttactgaa cggaattaca gaaataaata
     2701 attttaaagg aatggcaaga tatacaggac ccagcacgaa aattgcccgt aaattcgggg
     2761 aacccatttt aggaccggat aagtattttg aaaagaaaaa ttatcctccc ggtatgcatg
     2821 ggttaatgaa aaaaaggaaa aagcaatctg aatacggagt tcaacttcag gagaaacaaa
     2881 aagcgaagta cacctatgga cttttagagc gtcaattttt gaatacattt aaaaaggctg
     2941 cacgctcaaa aggtattacc ggtgagattc tacttcaact tctcgaatca cggctcgaca
     3001 atgtagttta caggttgggt atagctccta ccagggcagc tgctcgccag ctggttggcc
     3061 acaagcacgt taccgtgaat ggatcggtgg taaatatccc ctcctaccac gttaaggctg
     3121 gagatataat aggtgttcgc gaaaagtcca agtcgctcga gattatttcc gattcgctgg
     3181 caggtagtca taacacccga tactcatggc tggagtggaa taaggacgag atgtacggaa
     3241 aatttcttaa tgtcccagaa cggtctgaaa ttcctgagaa tattaaggaa cagctaattg
     3301 tagaacttta ctctaagtaa tctaaccaat accataatgg caatactggc atttcaaaaa
     3361 cccgataagg ttataatgct cgaatctacc ggcacaaccg gtacttttga gttccgtcct
     3421 cttgagccag gctatggtat cacggtgggc aacgctttaa ggcgaatttt gctttcctcg
     3481 ctcgaagggt acgccatcac ctggatacga attcgtggtg ttgaccacga gttttcaacc
     3541 ataccaggtg ttatggagga tgttaccgaa attattctca acctgaagca aatacgcttt
     3601 aagcgtattg tagatggaat ggatgaggaa aaggtaacca tcaccatcac cgggcaaaac
     3661 cagttcaaag ctggatacct ttccaacttt ttatcaggtt ttaaggtgtt aaacccggag
     3721 ctggtaatct gcagaatgga gccagatgtg aaaatcgaag tagatgttca cattggtaaa
     3781 ggacgtggat acatacctgc cgaggagaat aaacctgagg atgctgaatt cggcattatc
     3841 cccatcgact ccattcacac ccccattcgc aatgttaagt attccgttga gaattacagg
     3901 gttgaacaaa agactgacta cgagaagctt gttatcgaga ttgataccga cggttctatt
     3961 caccccaagg atgcattaaa ggaggctgcc aaaatcctta tttaccactt catgctcttc
     4021 tccgacgaga aaattaccct cgaaaccgag gataagtttg aaaccgaaga gtttgatgag
     4081 gaggctctcc acatgcgtca aatgctgaag cagaagctgg ttgacctcga cctttccgtt
     4141 cgtgcactca actgtcttaa agctgctgat gttgaaactc ttggcgacct tgtcaggtac
     4201 aataaaaatg acctgctcaa gtttagaaac ttcggcaaga aatcacttac cgagcttgaa
     4261 gacctgctcg acgggctcaa cctatcattt ggtatggata tttctaagta taaactggat
     4321 aaggattaaa tcgcgatgag acacaacagg aaagtaaacc atttaagccg tacaagcgct
     4381 caccgtaaag caatgctttc aaatatggcc tcctcgttga tactgcataa gcgcatcaac
     4441 actactgtgg ccaaagccaa agctttaaga atctatgttg agcccctgat aacaaaaagt
     4501 aaaaacgata ccactcattc cagacgcgtt gttttcagct acctgcagaa taagtacgct
     4561 gtaactgaac tttttaggga ggtttcgcaa aaggtagccg atagacccgg aggatacacc
     4621 cgtattctta aaaccggaac ccggctgggc gataatgccg atatgtgcat tattgagctg
     4681 gttgactata acaccaatta tgttaaagaa tcagcaggta aaacggaagc caaaagaacc
     4741 cgtaggggtg gatcaagagc taaaaaggag gaaacacctg tggttgaatc ccctaaatca
     4801 gctactagcg aagttaattc cgagatacct tcggaaggag aaatttctga agaaaaaata
     4861 tcagagtagc gaagctagca acatactatc agggtggggg atgagcagct tgctttatcc
     4921 ccttttttat ctaaatcatc aacttaaaaa aataaactat gggacagatt gttagtattc
     4981 acgcaaggga aattctcgat tccagaggaa atcctactgt tgaggtggat gttttaaccg
     5041 aaagtggtta ctttggtaga gccgctgtgc ctagtggcgc atctacaggt gttcacgagg
     5101 cagtagagct tcgtgatggt gacaaaggac gatatcttgg caaaggcgtg cttaaagccg
     5161 ttaacaatgt gaatacaacc atttcggaag aaattgtcgg catgcacgtg ttggaccagc
     5221 aggaaattga cgaggccatg ataaagctcg atggaactcc caacaaggga aacctcgggg
     5281 ccaacgcaat tctcggagta tccctggccg ttgctcaggc tgccgcccag atgaccacac
     5341 agcccttttt cagatatgtg ggcggtgtaa atgccagaac attgcccatt ccgatgatga
     5401 atatccttaa cggcggatct catgccgata atagcattga ttttcaggag ttcatgatca
     5461 tgccggttgg ggctgagtcc tttagcgagg ctattcgcat gggtgccgaa gtttttcata
     5521 acctgaaaaa cgtgctaaaa aaacttggat attcaaccaa tgttggcgac gaaggtggat
     5581 ttgcacccaa cctaaaatcg aacgaggaag ctgtaaaggt aattttgcaa gctatcgaaa
     5641 atgcaggtta ccgcccgggt gaggatgttt ttattgcact cgacccggcc tcctcagagt
     5701 actacctgcc cgaagaaaat gtttaccacc tgcataagtc tagcggcgat aagcttactc
     5761 ctgcccaaat ggttggctac tggaaagatt gggtaacgag gtacccaatc atttcgattg
     5821 aggatggaat ggccgaggac gactggagcg gttggaagct gatgaccgat acccttggta
     5881 gtaagattca actcgtgggc gacgatcttt tcgttaccaa cgtggaacgt ctccagaagg
     5941 gaattgacga gggagttgcc aacagtatcc ttgttaaggt taaccagatt ggcaccctaa
     6001 ccgagacaat caacgctgta aggcttgccg atactcatgc catgacggct attattagcc
     6061 accgttcggg tgaaaccgaa gatactgcaa ttgcacattt ggctgttgcc ctgaatacag
     6121 gcttaattaa aactggctcc gcatcacgct ccgatagaat tgccaagtac aaccaactcc
     6181 ttcgtattga agaaatgctg ggtccttctg ctatttaccc cggtcgtaac tttaagtaca
     6241 ttaaaaagta acggttgctt tctaaaaatt aaatcgaggg gttcggatta acatccaacc
     6301 ccttttttta aatttattgg caaaattttg atatggccta taaatcgtta caccacttta
     6361 tcaataagct cgaggagcaa aacgagctga taagaatttc agcttttgtt gaccctgtgc
     6421 tggaaatagc cgagattacc gacaggatgt ccaagcaacc caacgggggt aaagcgctcc
     6481 tgttcgagaa tacagggaca tcttttccag tgcttaccaa tgctttgggt tctataaacc
     6541 gaatttgttt ggccttgggc gtttctgggc tcgacgatgt tggctccgat attaataaac
     6601 tctttgccga gttggccggg cccaaaacaa acatttggga gaagcttggc acaattccaa
     6661 cgctaggtaa gctctcggct tggcttcccg ctcaaatcag ctcgaggggt gcttgccagg
     6721 aggttgtcca cgccgagcct aatatcaacc tgctacccgt tatgcagtgc tggccttttg
     6781 acggtgggcg tttcatcacc ctacccatgg taaatacaaa agaccccttc accggtattc
     6841 gtaacgtggg aatgtaccgc atgcaggttt tcgacccaaa acatacaggg atgcattggc
     6901 atcagcataa agttggagct cgacattttg aagcctacaa aaaattaggc aagcgcatgc
     6961 ctgttgccgt tgcgcttggt ggagatcctg tttataccta tgctgccacg gccccaatgc
     7021 ctgataacat ggatgagtac ttacttgcgg gttttctacg aaaaaaaaag gttaagctgg
     7081 tcaagtgcct gacggtagat ttagaagtcc ctgaggatgt ggattttgta ctggagggat
     7141 ttgtcgatcc aacggaagaa cttgcatggg aggggccatt cggcgatcat accggtttct
     7201 actctcttgc cgactggtac ccccgttttc acttaacctg cattacccat cgcagggatg
     7261 cggtttatcc tgctaccctg gttggtgtgc ctcccatgga ggatgcctat attgccaagg
     7321 caaccgaacg aatattttta cagcctctga gaattacaat gctaccggag ctggaggatt
     7381 tggctcttcc ggtggaaggc actgctcata acattgcttt agttaagttc agggattact
     7441 accctggcca ggcatacaaa ataatgaatg caatctgggg cgcagggcag atgatgttta
     7501 ataagctact ggttgctttg ccaaaagaca tagaccttac aaatgccgac caaatacttc
     7561 actcccttgt taactttgtt gaccccgaaa gggatttaat gtttggtaag gggcctctcg
     7621 atatccttga ccatgctact cctacacagg catttggaag caaactcttt attgatgcca
     7681 ccaaccaaac ggttgttatg ccccaccttc aaattagcat caatgatgta aaggagctac
     7741 ttgaaaggga aacttccagg gtgaaaaatt tcaacctgaa tcttctcaac aggggaattt
     7801 ccattcttat catttctgtt gataagcaag ccgtaacaaa tgtaaaatcc tatatgcagc
     7861 aacttttccg ttgccaggag ttggctggat taagggctat ggttgccgtt gacatgggtg
     7921 atgctgttac cgatttgtct atgctaacat ggtttgcttc aagtaacatc gacccgataa
     7981 gagatgtatt catactcaac ctgaacgatg gttacccagc ccgagttgga gttgatggca
     8041 cccgcaaggc ataccctcag gataatttta agcgcgaatg gccaaatgtt gtggccatga
     8101 gccgggaggt ggttgaccac gtaaacaaac agtgggaaaa atatggaatt ggcggaatgg
     8161 ttgaatcccc ctcgaaacag cttttacccc ttgtggctgg tgaagggccg gtggtaaagc
     8221 tcaacagctg aacttttaca ttgatatggc ttcaccataa tcactttttt gggactcata
     8281 gctgcattca acttactgtt tctgagttag cgctgagcat ccgcttaaaa tttcctcagg
     8341 agtaccgttt aatgctgcag gatataggct actacttggg ccaggtggtt atcatcctga
     8401 taaattcaag cctagttttg ttatgctgaa aaataagtat attagcgggt accatgagat
     8461 gtaaagctgt ttttaaatgc ttgttgccgg tgctgctttt ttttataaaa ggcggaggtg
     8521 aactttttgc tcaaaaaaca gaggttgaag tgttcgatgc taacaatggc ctgccccaaa
     8581 cctatgttag ctgtttaatt caggattcaa agggattttt atggcttgga acccatagcg
     8641 gcctttgcct atatgatggc taccagttca ctacttttag caacaaccct ttagattcat
     8701 tgagcctttg caacaacttc gttaaatcaa tagctgagga caaaaatggc gatatttggg
     8761 ttgccacact atcggggtta agccgctatg agcgggcaac cggaaaattt attagctact
     8821 tttccgaacc aaccaatcct gcatcccttg ctggggacaa aatttactac gtttacgtag
     8881 acagggagaa aaacctttgg gttcacacac ccgggacatt gaacctgtac agctataagt
     8941 ccaatgactt cactcgattt aactatttta acgacctctt tacccaaccg gttgaaaccg
     9001 atatataccc catttacgaa gacctgcaag gaagaatttg ggtgggtacc aaggatgggt
     9061 tgatgctgtt cgatagggaa agaatgcttt ttaaacgtta cagtagccga atatcctacc
     9121 cttactcaat atatgggaac agagttcaag ccatttatga ggatagtaga ggtaattttt
     9181 gggttggtac cgataagggg cttaacctat tcagctttaa gctgcagcga ttctatccgg
     9241 ttggggcagg catagatgaa aacgatgccg tcaggttcat ttttgaggat agccgctcaa
     9301 cccttttact tggaatggaa agtggggttg cgaggttaca ttcttctaca gaaaaagttg
     9361 agcagctaca aaatttttat agcaaaagca gcaaaatttt acctattggc atctctgcta
     9421 ttctaaagga taagtcggga attacctggg ttgcctcacg tacaggtttg ataaaaatca
     9481 acccctttag ccaacggttt aagggctaca gtagggacaa cttgggcaat ccgcttttct
     9541 ccaataacat tattgcatct gtttttgagg attcctccgg aaaactctgg gtaggaacat
     9601 ggggtagagg tctgttcatt ttcaatccga aaactggaga aaacctgaga tactgtaaaa
     9661 gttcatcaat ctaccgctta cccaatgatt ttgctcatac agtatttcaa accagaaagg
     9721 gtgaaattct tgtgggaacc cggaatggtg tctttgtgta tgaccagaga agcaggcagt
     9781 ttagcaattt ttttgccagt aggcatgtta gcgttggtaa tattttcgtc gataatcgcg
     9841 tgtattccat tgacgaggat tccgctggaa acctttggtt tgctacggcc attggtttgc
     9901 attgcttaag tggtaatcgc ctgcatagct actattcccg taggggtgat tccatatttt
     9961 taccttcgaa tttaattttt gatgtgctag ctggaaagca aggagaagtt tgggtggcta
    10021 ccgcaaatgg tttatcccgt atatcggacg attgttcaag cgtgattaat ataacggtta
    10081 tgaccgattc cgctggtcaa ggacaacaac ccctaggtga agtttactgc ttgcaccagc
    10141 tatcaaatgg agcaattctg gctggtacca gcaacgggtt ggttagcgtt aacttgttga
    10201 aagggagcaa aaacctttat ttcgaaaacc ttatcatccc atcaatatcc gcaagggcaa
    10261 ttgaggagga tgtaaagggt aatttttggc ttagcaccag caagggtatt acctgctata
    10321 acccatcaac agggcaactt gccagctata catctgacga cggcttgctg gggaacgagt
    10381 ttaacttgtt gtctagcttc cagtcgcctt cgggaacaat atactttgga gggatgcagg
    10441 gaattagcta cttccatccc gattcggtaa tgcttaatag ccatattcct ccattaagtt
    10501 ttactaaaat atccttagcg agcgtaagca gtaccaaaaa tattcaccct tttggtatag
    10561 atgaagtaag aatcccgcag ggatttagct cactgactat tgaattttcg gcgctggatt
    10621 ttaaccatcc cgagaaaaat ttataccggt acaggctaca gggacctggt agtagcaagt
    10681 gggttgaact tggcaacaag cattcaatca ccttaacgaa ctttagagag ggtgttttca
    10741 ggcttgaggt tatgggggct aacagcgatt gtgtttggac tactgaacct ttatccctcc
    10801 ggattattgt tgttgctcct ttttggcgat cacgtattgc atacactgtc tatgccttgc
    10861 ttttactatt cgtattgatc gcttatggaa gctatcgaag aagaaattca ctaaggataa
    10921 aaaggttgct tgcagagcgc gaggatgtta tggccaagct tgaacttcaa aaagaggagc
    10981 tggaaaataa gaacaaaaat ataaccgata gcattcacta tgctaaaagg attcaggatg
    11041 ccatgcttcc tactgttgac atgtttaaaa gccatattcc aaattctttc atcctcttta
    11101 aacctaagga tattgtaagt ggggattttt attgggtaaa ggaaactgat agtaaatgct
    11161 ttgttgccgt tgttgactgc actgggcacg gagttcccgg cgcattcatg tcaatcattg
    11221 gcattgagct gcttagaaac atcaccgagg ttgaggcagt tgaggatact gctgaagtgc
    11281 tgaataggct tagcgctagt gttcatgaaa ccttttcgtc ggttagctac cctaacgaaa
    11341 gtggacttaa ggttaaagat gggatggatg ttaccttttg cacctttgat aagcagtatg
    11401 acataatgca gtttacaggg gcgtttagcg atttgtacat tatccgtgat ggtaatatta
    11461 tcgaggttaa gggcgacaga ttttctgttg gtatggctga tgatgcaggg cgctacatgt
    11521 ttagcagctc atacatacct ttaatgcccg acgatatgat ataccttttt accgatggtt
    11581 acgttgacca gtttggtggg cccgagggga aaaaatttaa gtttcgtcgc ttccgccgcc
    11641 tgctgctcaa catttatagg ctaccccttg aaactcagca gaaaatactt gaggaaagta
    11701 tcacggaatg gatgggcggt caggaacagg ttgacgacat cctggttatt ggcattcgta
    11761 ctcctactaa ggcaaagtag catgtagggt taagctgcta gctacttgcc tgaggcggca
    11821 tgttgatcat ctaacccttt taatgccatc taaacgattc taatttgttt ctagttatga
    11881 agcacccatg taggacggct atcacaaaca cgtatgaaca accgggcgtt acttgcgggt
    11941 tcgacgaagc caaaaatgga tgttccatat atttaaaccc gatgcctaaa gggtatagaa
    12001 gtcagaaatc taaaaaattg gtaaggatga aacgattagc aatcctgttg ctgttctcga
    12061 ttttaactcc cattcagaat tttggccaaa cagttagcct tgtgcttagc gggggaggag
    12121 ccaaaggatg tgcccacatt ggtgtaatta aagcgctgga ggaaaacggg attcctattg
    12181 ataatgtttc gggaacatca ataggggcga ttgttggtgg cctatacgca atgggctatt
    12241 cacccgatga aatggttaat atttttaagt ctagagattt ttacaactgg agcaatggcc
    12301 tcattgatga aagctataag tttaacatca acgatttgtc aactaccgat gccgagaacc
    12361 tgagcgtggg gtttaccttt gacagaggcg ggttaaaaac taagcttacc tccagcatag
    12421 taccgccggt agggctggat attgcctttg aggaaatgtt tgctcaggga acggcgattt
    12481 ccagaggaaa ttttgacaaa ctttttgttc cttttaggtg caacgcctcc gatgtggtaa
    12541 acaagaagca ggtttacttt agagagggta accttggtaa agcaatccgg acatccatga
    12601 cattcccgct ttacttcacg ccagtttata ttgattccct actactgttt gatgggggaa
    12661 tatacaacaa ttttttatgg aaggaagcgc tgcaggagtt tcatcccaac attattgtgg
    12721 ggagtaaggt ggcctctaac agcaagctgc ccagcgatga agaccccttg cttcaagtcg
    12781 aggctatgat tgttggcatt accgattata gcattcccga ttcgttggga atagttattg
    12841 atacccggtt ggagaatatt gacctacttg atttcgataa ggttgatgaa attgtttctg
    12901 cagggtacaa cgaaacactt aaggttattc ccaagttgag ggaacaaatt acaagaaatg
    12961 ttaccccgga ggagttggcc atgagaagga aaaactttcg tcagaatctt cccccgctat
    13021 ttattgacaa catccgggtt ggaaaccttt cggacaggca gaaaacctac ctgcacaagg
    13081 taatttttaa cgagtactca attgttacct ttgataagtt taaaaaggat tactaccgct
    13141 tggtatccga tagaaccttt aagcggttga tacccgagtt cgaattcgac tacacttcgg
    13201 gactttttga tgtagcttta aatcctaccc taaataggtc tgttgatttg ggcctgggtc
    13261 ttagcctttc ttcggatgtg ggcaacgagg ggtttgcttc tgcaagctat aaatggctat
    13321 cgcgaacctc caacacaatc tatggaaata tttactttgg aaagctgctc agcagtattc
    13381 gcctcgacta catcaagaca ttccctacca aaataccagt atcgttggtt gcctgcacta
    13441 ttgccagcag gtatgattat cacagcagca atccaattcc gttttttgag gatgttaaac
    13501 cgccttatgt tctgcaggat gaaacatttg caactgtatc gctgaaagtt agcaatacca
    13561 gcgcaaccac tacaacgcta tctgtatcgg gaggagaaaa ggttgaccac tactaccaag
    13621 ctatggatta ctactctttt gatgttcctg accaaaccac gtttaaattt attaagggtt
    13681 cgctttgttt cgataagtac accctggatt ctaaacagta tgccacacgg ggtaggcatc
    13741 agcaactagt aatatcggga ttttcaggta gagaggagca ctaccccgga acaaccgccc
    13801 cccagatgac cggagtaaga aaaaaccact cctttgcaac attttatctt tacaatgaaa
    13861 gctaccatcg tatttggaga aggcgattct ggctggagct gttatttgag tcctactact
    13921 caacccaagg gctttttagc aactactttg catccatcct tacttccaac cagttttcgc
    13981 ccaccccaca ctcaaacctc atttttatag aaaattaccg cagtactcag tatgctgctg
    14041 taggtgtagc tccaattttt gagttctgga aaaacgtaca ttttagggtt ggcgcttacc
    14101 tttaccagcc atttagaacc ctaaagcagg atgatgtggg agttgcaaat tatagcgatt
    14161 attttgctaa gcgatggtta attggatcgg cttcgttggt ttatcggaca cctgtaggcc
    14221 caattgcctt aacactaggc tactatccaa ctgccaacag caatggagac gatattttct
    14281 tcaacctaac ctttgggtac tcaatcttca atagcagggt gtttgataac taaacagtta
    14341 cccgctactc cagaaatttc ttcattttct gaataagcac ctctatctgg aatggcttgc
    14401 ttatgtaatc gtccatacct gccgctaggc tttcctccct gtcgcctaaa agggcgttgg
    14461 ctgttattgc aatgatgggc gtatggctgt tggtgctagc ctcgatctcc ctaattttgc
    14521 gagtggcaac atagccattc ataatgggca tctgaacgtc cattagaata atgtcgtatt
    14581 gactggtgcc aaatttgtcc aacgcctctt ttccgttggc ggccacatca atattcttta
    14641 ccagcttttt caggcttaga atcactattt tctggttaat caggttatcc tctaccagca
    14701 gtacgtttgc attactaagt ttaccgtcgc ccttagcctc cgccgagggt tgggcagtaa
    14761 gggtagaaat ttcttcatgc tgtctttggg tagttttaag aacttcgggt tcagctactt
    14821 ttttaatttc aggtaggctg ttcaaaaagt aggtaaagct aactacagca tcgttagagg
    14881 tcagcttgat gttgagtttt ccgccgttgc tctgtaccag attgttcgtt atggtcaggt
    14941 ttagcaggtc aatgaaaaat tggttgttta atgaagagga ggttgaatcg gcaattacag
    15001 tttgcgaata cccctcattt tggtggatga gtaggggcac gttgcttttt acctcaaaaa
    15061 atagctcgag ggagtttttg gcattgccca tccggctggc aataatctcg atgttaacct
    15121 tgcttccctc cttattcttg attacattct caataatgtt gagaaaaatt tgtttcagct
    15181 taacggggtc gccctttagt gttttgggaa tactttcatc aattttaatg ttgaagttaa
    15241 ccgtctgcga ttgcgtggca aataggcgaa tagtgttgtt gatagtgtca gataggttaa
    15301 agggaatggc aaaatctgtt tcaggctcta ttcccgtgct cgatttctcc accatgccgt
    15361 ttactacacc tgccaagctg ttagtggatg cctgaatggt gtcaatcaga tccctgtact
    15421 tgtcctcggt gatggtgttt gacagcatgt tggatatgac catgatgttg ttcaggggtg
    15481 tgcgaa
//
LOCUS       DAOPHX010000056        15209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_4469_length_15209_cov_6.721592, whole genome shotgun sequence.
ACCESSION   DAOPHX010000056 DAOPHX010000000
VERSION     DAOPHX010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 15209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15209
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4469_length_15209_cov_6.721592"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PK502_05695"
     CDS             complement(<1..192)
                     /locus_tag="PK502_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00342.1"
                     /translation="MKRILLAVAIIWVGLAFTARNANAQSEEELVQICGMIAGEAQYL
                     KDFRVKLDAGDPPPVQRIPI"
     gene            complement(207..1130)
                     /locus_tag="PK502_05700"
     CDS             complement(207..1130)
                     /locus_tag="PK502_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00343.1"
                     /translation="MKSRFPIVIVAICSLSIALSSCKSCNKDKDNLGEELVIPDSLKW
                     DTPLPMGKPIVDELMQNISSPVETAALIKGLKIPFNKDYMASTKQEESFNTSFDKALA
                     LGIYGCDLGYLNMYEKTSFVVDYISVIKRFADDIQVGQFFDFETLKRLATNKENLDSL
                     ILISQQSFNRIDSYLRETNRSTLSAVIVAGVWVEGAYLTSRVAQTSNHPRIRETIGEQ
                     KTVLGFLLALLKAYSNDPNMAKLIAQLEPLKNQFDNVKITYELGEPEMIEVNGELTFI
                     QKDKQLVDMDDVTYKEIISGIQTLRNSLIKL"
     gene            complement(1177..4275)
                     /locus_tag="PK502_05705"
     CDS             complement(1177..4275)
                     /locus_tag="PK502_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPH00344.1"
                     /translation="MSEEILRALMQLFAIIAKQDEGVEMKERDYVETFLTQQLNDEAV
                     AEYLRLFDEHAGLLDKEIGSDDEERSKPKLTSVKDSVRILGICKKINKTLTQKQKIVV
                     LVRLYELINADRKFTEQRMAIINTVAEVFKLSHEEINDIENFVVRNNPEELDRPSILT
                     IHDRPFKGEQCKHIPTEALDGFIYILQIKSEDLYFLRYTGNEDIYLNGLPISNRRIYL
                     FASGSSLKLPKGKPVYYTDVVAHFLSDSTTIRISYRVDNASYTFKNGTVGLHNISFSE
                     GHGKLIGIMGASGAGKTTLLNVLAGIEKPTSGHVYINGYDLHASSNMLEGVIGVIPQD
                     DLLIEELTVFENLYYNAKLCFKDKTEPELIAMVDKTLSNLGLLEQKNLKVGTPLNKMI
                     SGGQRKRLNIALELIREPSIMFVDEPTSGLSSRDSENVMDLLRELTLRGKLIFVVIHQ
                     PSSDIYKMFDNMIILDTGGYMIYYGNPVEAVMYFKRLDMQINSEVGECPVCGNVNPEL
                     LFNIIEARVVDEFGRYTPIRKVSPQKWEEHFASNIKPPVIPDEKVEPPRTLNIPSRFK
                     QFTIYTMRDLRSKISNSQYIILNLLETPILAFILAYVVRYIADPNSKIYIFRENENIP
                     IYIFMALIVALFIGLTVSAEEIFRDRKILKREAFLNLSRSSYLLSKVAILFLLSAIQS
                     ATFILIANSILEIRDMYFDYWLVLFTTAAAANLLGLNISATFNSAVTIYIVIPLVMIP
                     MMVLSGAMFSFDKLNRSIGSFNRVPVIAELMITKWGYEALIVHQFKDNEFEKNFYEIE
                     KNLSNANFKVTYYIPELEKRATDALDLLQQEKSKANQEALKDKLAVIRKAVITETTVT
                     APSVPFAYTQKLTPEQFDEETAYALIDYLDALHRLYSDIYQTQNQKRDRIINYLVNTN
                     EKLYQAKRMAYHNESISDLATKVFEKNKILQYGDELVQQYDPVFRDPLPRHILDIRSH
                     FLAPRKHFLGMYFDTFWFNIIVNWIMSIILYITLYYESLKKLIELFSKIKVRGFFKAK
                     IS"
     gene            complement(4366..4749)
                     /locus_tag="PK502_05710"
     CDS             complement(4366..4749)
                     /locus_tag="PK502_05710"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPH00345.1"
                     /translation="METIKIMGTDDTPTVILDAENEIFEISGRSLPEDVTAFYDPVLS
                     WLDGYASSPNPKTIFTFKLVYFNTASSKLLLDVLMKLEQMHEAGHDVLVRWYFPEDDE
                     DMQEAGEEYADIVDVPFEQVSYTLS"
     gene            complement(4761..5336)
                     /locus_tag="PK502_05715"
     CDS             complement(4761..5336)
                     /locus_tag="PK502_05715"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HPH00346.1"
                     /translation="MDTKNVKGFLEFVYDFYKSMKAHEITLVYEGEITHQITKAFTSL
                     TESNMAKEEEANSVQKKVFHVMVECLQNISKHADSFGSDDFLFAGRGIFMVSKGDAEY
                     HVTTGNIIENSKIEELSQILDNINNLDKEGLKELYKTQMREGRLSEKGGAGLGFIDIA
                     RKTGRKLDYHFLPIDEDTHFFVLTSTISRTE"
     gene            complement(5376..7988)
                     /locus_tag="PK502_05720"
     CDS             complement(5376..7988)
                     /locus_tag="PK502_05720"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPH00347.1"
                     /translation="MTSFRLTSVRETKNLTRNKIALAFLVGLLFSFAAWIADFSKSGL
                     TFSLQNIFRQHAANPTLWITDLIPFAFAYITWLQSTRFMRKTLRLEKELKQRDEDIKK
                     NADFAKKIGEGNYNAPFEVASEDDILGKSLLIMRDNLLTNQKKEWERSWIAKGKEEIS
                     YILRLHHNIDELSYDVLVKLIKYINIIQGALYIYDEEKQLLINMTTYAYNRRKYINQE
                     FRIGQGLIGECAYEKDFIYRTEIPDDYITITSGILGDKKPKSLLLIPLITDEKLEGVL
                     EFASLENEIPELTIQFLKEVGEIIARTIFNLRINQKTERLLQEAQQMTQELRENEEEL
                     RQNAEEMRATHDELEKSNQQLEAKIKEVENAQKRLHSLLENASEIISIYNSEKKLTYI
                     SPSVTKILGYSPQEMMSGKDFDRLTRKGESDFNEMFQQLIENPHIAITIQYTFMKKDG
                     EKIFLEVTGRNLLDDPAIGGIIINSRDITERKRAEKEERMRSKMQSLSENSPDIIMRI
                     NTQGQFFYANPMVEQYLGISYRDLVNQTLTALDISPTLSGFIKDAIKNIKVNKEKVEA
                     EITFASQMGETIMHIAAIPEFNENELETILFVGHDITEAKRIQMEIHDKNRKITESIN
                     YAQRIQTSILPNNRIIRQYLPKSFIYYHPRDVVSGDFPWFFTKDDYIYIAAVDCTGHG
                     VPGALLSFIGYFTLNNVVDHDSSYTAGEILDRLHYGVRKTLKQDRPDADARDGMDIAF
                     CKINPKTKELQYSGAHRPLYLMRNGELIEYKGNRKAIGGIPHPKKAEEDFVNHLIQIQ
                     PGDRIFFFSDGIVDQVGGPDKRKYSSQRIRELITSNPQTPIQDMAKVFSQDFDTYKGD
                     LKQVDDILLIGIEF"
     gene            complement(8031..8846)
                     /locus_tag="PK502_05725"
     CDS             complement(8031..8846)
                     /locus_tag="PK502_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPH00348.1"
                     /translation="MKAFSSKSKLLPLLLLVLLIAGVVITFGLIHNTLQNTGGSLPAI
                     GYVLLFLPAIISIGLYAMLNNLQVKSAKLEAEIAELKAAFALLQQVKNQEQANATNQE
                     ETVDYDKLLSRLIPTLPTDNLEKFGEALLSNIARNYEIVQAVLYTRDGDTNLFTFKAG
                     YAYFSESEPVTYSEGETLSGQVAKNRQILNLDQVPENYITILSGLGKGSPKHLLIAPI
                     VSPDNNSIGVFELAAFKPFDNMQVELFDRLGRKLGELLWSSRPETYLKHSAWT"
     gene            complement(8851..9411)
                     /locus_tag="PK502_05730"
     CDS             complement(8851..9411)
                     /locus_tag="PK502_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HPH00349.1"
                     /translation="MYKAVIIGGSAGSFQVITRILHSLPASFPLPVLLCLHRLKHVRS
                     GFVEALSLKSGIPIIEPDDKEQIKPGKAYLAPANYHMFIELGNKIALSTEDPVNHSRP
                     SIDLSFLTAAQVYRDKLIGVILSGANRDGAYGLKKIKDLGGLAIVQDPNECQVRTMTE
                     ASLKMTPVDHVYNTSEIIRFLQTIKT"
     gene            complement(9417..10250)
                     /locus_tag="PK502_05735"
     CDS             complement(9417..10250)
                     /locus_tag="PK502_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HPH00350.1"
                     /translation="MMQYEIGIVETRNVIKTLLDDYGYDFRDYALTSFKRRLEQIISN
                     NGLKDADGLIGKLKGNPTFFDQFLNEVNIDTTEMFRDPSFWRTLRDDILPEIIRNSTT
                     KPRIWIASFDSGEELYSLTIILKEMGALDKVQIYSNVFTNATIQKITSGQLDPKDIEI
                     NEANYVRLNGKSQYSSYYKVEGNKYFFDSTLVKDVNFIKQTPIIDKDPGAVKLVLFRN
                     QALYYNQLLQERVLQKIAESLVAGGFLALGIKETLENTNANNKFTVFNETERIYKRKN
                     V"
     gene            complement(10397..12754)
                     /locus_tag="PK502_05740"
     CDS             complement(10397..12754)
                     /locus_tag="PK502_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPH00351.1"
                     /translation="MKISQRIQLIIVPLMILLFSASGVTLNYLSKNRVRESIRIQEGV
                     YIDKLIQIVDMVEAKTGSGLSSDDIFELRTYFLSPAYYSTDYPAIISTNGVYLLHTTL
                     QGQRLPSAATAQIRKSKSTDGYIYYDDYSGNKPEKRLLCYKFYKPYNAYIAISVSMNE
                     ALAPMKSNRMAIIVFIILASLLTAFAIPLLLKPIIRIINLVNIKLRQLAKGDIPNPIK
                     ITSKDELGEMVTSLNQLMEGLKSTTSFAHQIGQNQLEHTFQPLSSKDELGNALLDIQS
                     NLKHALEEEQRRKEEDYIRGWVNEGLAKFGDILRQNNNNLTLLADSIIQNLVNYLEAN
                     QGGIFIYNDSENDKHLEMLSAFAYNRKKFISKTIPLGEGLVGACALEKKTIYLKEIPA
                     DYIAITSGLGEATPTALLIVPLKLEDEIFGVVEVASFREFKPYEIEFVEKIGESIAST
                     LFAVKNSIRTNMLLEQSQQQREEMAAQEEEMRQNMEEMQATQEEMLRKNIELELITNT
                     INQSLLSLTLSEEGYIADSNQNFVETLGYSKTELEGKMLADIVNPEQRTDFQISWKKV
                     RAGQTITETIHVLNRNGNSHYLMVTVSPELDPAGAITKVLLIGQDITEMKQLEIKAQR
                     QAEEIEQNLKQLEKEQELSAQRQKDMSTLLEALDQNCLVSIIEPDGLITYINNKNVEV
                     LGDKKEDIEGKFLHDIDYSAKHRPAEYKKFWDNILNGKKQQREFSLTVKGEVKWVLEN
                     FTPVMDESGKLYKIINIGFDITENKNKEAELTKIIEQMKNKPKNL"
     gene            complement(12930..13769)
                     /locus_tag="PK502_05745"
     CDS             complement(12930..13769)
                     /locus_tag="PK502_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HPH00352.1"
                     /translation="MQVTDQDLQYFITTLKNVSKYDFSEYSDKSLKRRLQKILEDYNL
                     DIFSLIANLKNDRAFIEKVVKEVTVNTTELFRDPPVWHLLRSRILPRFRNNKTINIWH
                     AGCSTGQEVYSMMILLNEMGMLEQAKIFATDINIDVLDAAKQGVYKYRFNLGYLDNFD
                     KVIKQNPLNFEEYNEVPYEKYFEIDKVKDTITMNKFLSEKPIFRKHDLVTDGNIFFAK
                     FDLILCRNVIIYFNYSLQNKVFELFHSNLYPKGVLLLGMHETILGPWATKFEKLGQSY
                     VKK"
     gene            complement(14138..>15209)
                     /locus_tag="PK502_05750"
     CDS             complement(14138..>15209)
                     /locus_tag="PK502_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00353.1"
                     /translation="LKMFGRILKTIGKVGSFLIIFLVFFLLYSSITYFDPESRVVLWQ
                     SDKPDTIPFDSTLAALTWNIGYAGLGSNMDFFMDGGGKMRDTRERTTENLTAIEKTLG
                     GEKINDFILLQEVDINSHRSYGINELEEISRTLEYPYSIGINYRVNFVPVPLKNPMGK
                     VQSGIVTFSKFNPKETIRYAYPGAFPWPNRLFNLRRCMVVNRYPTSNGKDFVLVNTHN
                     SAFDNGALKQQEMDFLQEILLSEYYKGNYVVAGGDWNQLPPGAPYAELVVGSSNSSFM
                     LTGIDSSFMPKDWSWVFDPSCPTNRYLNEPYTPGRTNTCILDFFLVSPNVSVQRCQTA
                     NMAFKNSDHNPVTLEFRLIGNP"
BASE COUNT         3709 a         3165 c         3680 g         4655 t
ORIGIN      
        1 aatgggaatt cgctgcacgg gaggcggatc gcctgcatcc agcttaaccc tgaagtcttt
       61 aaggtattgt gcttctcctg caatcattcc gcatatttga accaactcct cttcggattg
      121 agcattagcg tttcttgctg taaatgctag acccacccaa ataattgcaa ccgctagtaa
      181 tatccttttc atggccaaaa aatttcctac aatttaatga gcgaatttct aagggtttga
      241 atacctgaaa taatctcctt ataagttaca tcatccatat caaccagctg cttgtccttc
      301 tgaataaagg taagctcccc gttaacttca atcatctcgg gttctcccag ctcataggta
      361 attttaacgt tatcaaactg gttcttaagt ggttcgagtt gagcaatgag ttttgccatg
      421 ttggggtcgt tgctgtatgc cttaagcagt gccagaagaa accccaaaac ggttttttgc
      481 tcgccaatgg tttcgcgtat gcggggatgg ttactggttt gcgcaacacg ggaagtaagg
      541 taggcgcctt cgacccatac ccccgcaaca ataaccgcac ttagcgttga acggttggtt
      601 tcgcgaaggt agctatcaat ccggttaaaa ctttgctggg agatcaggat gagggagtcc
      661 aggttttctt tattggttgc cagtctttta agtgtttcaa aatcgaagaa ttgacccacc
      721 tgtatgtcgt cggcaaagcg tttgatcacc gagatgtagt caaccacaaa cgatgttttc
      781 tcgtacatgt tgagataacc gagatcgcaa ccataaatgc ccagggctaa agctttgtcg
      841 aaactcgtat taaacgactc ctcctgcttt gtgctggcca tatagtcctt gttgaaagga
      901 attttcagac ctttaatcaa ggcagctgtt tcaaccggcg acgatatgtt ctgcatcagc
      961 tcgtcaacta taggcttgcc catgggtaaa ggtgtatccc attttagcga atccgggatt
     1021 accagctcct ctcccaggtt gtctttatct ttgttgcacg atttgcaaga ggatagggca
     1081 atgctaaggg aacaaatggc aactattaca attggaaacc tgcttttcat gttgcgttcc
     1141 tgtttaaggg gaaatgtcgt aaatttcaaa gtaaagttat gaaatttttg ctttaaaaaa
     1201 gcctcgcacc ttaatttttg aaaataattc aatcagcttt ttaagcgatt cgtagtagag
     1261 ggtaatgtac aggattattg acattatcca gtttactatt atgttgaacc aaaaggtatc
     1321 aaaatacata ccaagaaagt gttttcgtgg ggcaaggaag tgagatcgga tgtcaagaat
     1381 atgtctgggg aggggatctc gaaaaaccgg atcatactgc tgcaccagct catcgccata
     1441 ctgaagaatc ttatttttct cgaaaacctt tgtggctaaa tccgagatgc tctcgttgtg
     1501 gtaggccatg cgctttgcct ggtatagctt ttcgttggta tttacaaggt agttgattat
     1561 cctgtcgcgt ttctgatttt gggtttggta gatgtcgctg taaagcctgt ggagcgcatc
     1621 aaggtagtcg attagggcgt atgcggtttc ctcgtcaaat tgctcgggtg taagcttttg
     1681 ggtgtaggca aagggtaccg aaggagccgt aacggttgtt tcggttatca cggctttacg
     1741 aataacggcc agcttgtcct ttagggcctc ctggttggct ttgctttttt cctgctgaag
     1801 caggtccaag gcatcggttg ctcttttctc tagctcaggt atgtagtagg ttaccttgaa
     1861 gtttgcattg ctaaggtttt tttctatttc gtagaagttc ttttcaaact cgttgtcctt
     1921 gaactggtga acaataaggg cttcgtatcc ccatttggta atcataagct cggcgatgac
     1981 gggaaccctg ttgaagctgc caatagaccg gttgagctta tcgaagctga acatggcgcc
     2041 tgaaagcacc atcatgggga tcatcaccag gggtattact atgtaaatag ttacagcaga
     2101 gttgaaggtg gccgaaatgt tcaaccctag caggttagct gctgcagccg tggtgaatag
     2161 caccagccag taatcgaagt acatgtccct gatttcgaga atgctgttgg cgattaggat
     2221 aaaggtagcg gattgaatgg ctgatagcag gaataaaatg gcgaccttgg aaagcaggta
     2281 gctcgaccgg ctaaggttga ggaatgcctc tcgcttgagt atttttcggt cgcggaaaat
     2341 ttcttcggcg ctaacggtaa gtccaatgaa caacgctaca ataagagcca tgaagatgta
     2401 gatgggaata ttctcatttt ccctgaagat gtaaatctta gagttgggat cggcaatgta
     2461 gcgcaccacg taggccagga tgaaggcaag aattggggtc tcaagcaggt taaggatgat
     2521 gtactggcta ttgctaattt ttgaacgcag gtcgcgcatg gtgtagatgg tgaactgctt
     2581 aaagcgcgag gggatgttga gggtgcgtgg tggttcgacc ttttcgtcgg gaataaccgg
     2641 aggcttgatg ttgctggcga aatgttcctc ccatttctgg ggtgaaacct ttcgtatggg
     2701 ggtgtagcgg ccaaactcat caaccactct tgcctctata atgttgaata gcagctcggg
     2761 gttaacatta ccgcaaaccg ggcattctcc aacctcgctg tttatttgca tgtcgaggcg
     2821 tttgaaatac atcacagctt caaccggatt gccgtagtag atcatgtagc ccccggtatc
     2881 gaggatgatc atgttgtcga acattttgta aatgtccgaa gagggttggt ggatgaccac
     2941 gaaaataagt tttcctctaa gggtaagttc cctcagcagg tccataacat tttccgagtc
     3001 gcgcgatgaa agccccgagg tgggttcatc cacaaacatg attgagggtt cgcggattag
     3061 ctccagggca atgttaagcc gctttcgctg ccctcccgaa atcatcttgt tcaggggggt
     3121 gccaaccttt aaattctttt gctcgagcaa gcctaggttc gatagggttt tatcaaccat
     3181 ggcgataagc tcaggttcgg tcttatcctt aaagcaaagt tttgcgttgt agtatagatt
     3241 ttcaaaaact gtaagctcct caattagcag gtcgtcctgc gggataacgc caataacacc
     3301 ttcgagcata ttgctactgg cgtgtaggtc gtagccgttg atgtaaacat ggcctgaagt
     3361 aggcttttcg atgcctgcca gcacgttaag cagagtggtt ttcccggcac cactggcgcc
     3421 cataattcct atcagcttac catgaccctc gctgaagctg atgttgtgca ggccaacggt
     3481 tccgttcttg aaggtgtagc ttgcattgtc caccctgtag gatattctga tagtagtgga
     3541 gtcagaaagg aagtgggcca ccacgtcggt gtagtacaca ggcttgccct tgggcagctt
     3601 cagggagcta ccgcttgcaa acaggtagat acgacggttg ctgatgggta gcccgttcag
     3661 gtaaatatcc tcattcccgg tatagcgtaa aaagtaaagg tcctcgcttt tgatttgaag
     3721 gatataaata aatccgtcga gagcttcggt ggggatatgc ttgcactgtt cgcccttaaa
     3781 aggtctgtcg tgaatggtta gtatgctggg tcggtctaac tcctcgggat tattccttac
     3841 tacaaagttt tctatgtcgt taatctcttc gtgcgaaagt ttgaaaactt cagcaacggt
     3901 gttaataatg gccatgcgtt gttcggtaaa cttacggtcg gcattgataa gctcgtagag
     3961 gcgaaccaga acaactattt tttgtttttg ggtaagagtt ttgttaattt tcttgcagat
     4021 gccgaggatg cgaacggaat cctttaccga tgtgagttta ggttttgacc tttcctcgtc
     4081 gtcggaccct atctccttgt ctaaaaggcc tgcgtgctcg tcgaaaagcc taaggtattc
     4141 tgctaccgct tcgtcgttaa gctgctgggt gaggaacgtt tcaacgtagt cacgttcctt
     4201 catctcaaca ccctcgtcct gctttgcaat tattgcaaag agctgcatta aggccctaag
     4261 tatctcctcg ctcatctttt agatcatcgg taaattgcaa acagccccca aaagatatgc
     4321 aggcttaaac ggaatcggca ttggcataaa tatgcaagaa gtatgctatg acagtgtgta
     4381 gctaacctgt tcgaaaggaa catcaacaat atcggcatat tcttcgcctg cttcctgcat
     4441 gtcctcatca tcctccggga agtaccatct aaccagcacg tcatggccag cttcgtgcat
     4501 ctgctcaagt ttcatcagca cgtcaagaag aagcttggag ctggcggtgt tgaagtaaac
     4561 cagcttaaag gtgaaaatgg tttttgggtt gggggacgaa gcatagccat cgagccaaga
     4621 taggacaggg tcgtagaagg cggtaacatc ttcgggaagt gagcgaccgg aaatttcaaa
     4681 aatttcgttt tcggcatcca gtataacggt tggggtgtcg tcagtaccca ttattttgat
     4741 tgtttccata ggtttagtat ttactcagtt ctcgaaatgg ttgatgttaa aacgaaaaaa
     4801 tgggtgtcct catctatggg caggaaatgg tagtcgagct tccggcctgt tttacgggca
     4861 atgtcaataa atcctagccc tgctccacct ttttcggaga gccttccttc cctcatttgg
     4921 gttttgtaaa gttccttaag tccttccttg tccagattgt tgatgttgtc gaggatttgg
     4981 gacaactcct caattttgct gttctcaata atgttccctg tggtaacatg gtactcggca
     5041 tcgcccttgc tgaccataaa aatgccgcga cctgcaaaaa ggaagtcatc cgaaccaaag
     5101 ctgtcggcat gcttgctgat attttgaagg cactccacca tcacatggaa tacctttttc
     5161 tgcaccgagt tggcttcttc ctccttggcc atattcgatt ctgtgaggga ggtaaaggct
     5221 ttggttattt ggtgggtaat ttcaccctcg taaaccaggg tgatttcatg agctttcatg
     5281 gacttgtaga agtcgtaaac aaactcaaga aaccctttga catttttagt atccatagcc
     5341 cgtaaaatta tataatttac tatcaaatag caatgttaga attcgattcc aattaaaaga
     5401 atatcatcaa cttgctttaa atcacccttg tatgtatcaa aatcctgaga gaaaaccttg
     5461 gccatatcct gaatgggggt ttgtgggttg ctggttatca gctccctgat tctctgcgag
     5521 ctgtacttcc gcttgtctgg tccaccaacc tggtcaacta ttccgtcgga aaagaagaat
     5581 atcctatcgc ccggctgaat ttggatgagg tggtttacga aatcctcttc agcctttttg
     5641 gggtgaggaa tcccacctat agcttttcgg ttgcccttgt actcaattag ctccccgttt
     5701 cgcattaggt agagtgggcg atgggctcca gaatactgta gctccttggt tttagggttg
     5761 attttgcaga atgcgatgtc cataccatct cgggcatcag catcggggcg atcctgtttc
     5821 agagttttac gaacaccata gtgaagtcgg tcaagaattt cgccggcggt gtaggacgag
     5881 tcgtggtcca ccacgttatt cagcgtaaag tatccgatga aggagagaag agctccggga
     5941 accccatggc ctgtgcagtc cacggcggca atgtatatgt agtcatcctt ggtaaagaac
     6001 catggaaaat cgccacttac cacatcgcgg gggtggtagt aaatgaaaga cttgggaagg
     6061 tactgcctga taatcctgtt gtttggcagt atggaggttt gaatgcgttg tgcgtagttt
     6121 atgctttcgg taatcttacg attcttatcg tggatctcca tctggatgcg cttggcctcg
     6181 gtgatgtcgt ggccaacaaa gagaatggtt tccagctcgt tctcgttgaa ctctggaatt
     6241 gcagcgatgt gcataatggt ttcgcccatt tgggaggcaa aagtgatttc ggcctccacc
     6301 ttctccttat ttacctttat atttttaatg gcatccttga taaatcctga tagggtaggg
     6361 ctaatatcca gggcggtaag ggtctggtta accaggtccc tgtagcttat acccaggtac
     6421 tgctccacca tggggttggc atagaaaaac tggccttgcg tgttgatgcg catgatgata
     6481 tcgggggagt tttcggagag cgactgcatc ttgctacgca ttcgctcctc cttttcagct
     6541 ctttttcgct cggtaatatc cctggagttg atgatgatgc caccaatagc tggatcgtcg
     6601 agcaggttgc gcccggtaac ctccagaaaa attttctccc catccttttt cataaaggtg
     6661 tactggatgg tgatggcgat gtggggattt tcgataagct gctgaaacat ttcgttgaaa
     6721 tcgctttccc ccttacgggt tagccggtcg aagtccttac cgctcatcat ttcttgtggg
     6781 gaatacccga gtatttttgt aaccgatggg cttatatagg tcagcttttt ttcgctgttg
     6841 tagatggaaa tgatttcgga ggcattctcg agcagggagt gcaaccgttt ttgggcgttt
     6901 tcaacctctt ttattttagc ttcaagctgt tggttcgatt tttcgagttc atcgtgcgtt
     6961 gcacgcatct cctcggcgtt ttgtcgaagt tcctcctcgt tttcccgaag ctcttgggtc
     7021 atttgctgag cttcctggag caaccgttct gttttttgat taatacgaag gttgaagatt
     7081 gttcgggcaa ttatttctcc aacttccttg aggaattgga tggtgagttc gggtatctca
     7141 ttttcgaggg atgcaaattc cagaacgcct tcaagtttct catcggttat aagtggaatt
     7201 aaaagaaggc tcttgggctt tttatcgccc aggatacccg aggtaattgt aatgtaatcg
     7261 tcaggaattt cggtgcggta aataaaatcc ttttcgtagg cgcattcccc aataagccct
     7321 tgtccgatac ggaattcctg attgatgtac ttacgccggt tataggcgta agtggtcatg
     7381 ttgatgagga gttgtttttc ctcatcataa atgtagaggg ctccctgaat tatgttaatg
     7441 tacttgatga gcttaaccag cacatcgtag gatagctcgt caatgttgtg gtgaaggcgg
     7501 aggatgtagg aaatttcttc tttccccttg gcaatccagc tacgctccca ttccttcttt
     7561 tggttggtaa ggaggttgtc gcgcatgata agcagcgact tgcccaaaat atcgtcttcg
     7621 ctggcaactt caaatggtgc gttgtagttg ccctctccga ttttttttgc aaaatcggca
     7681 ttctttttaa tgtcttcgtc gcgctgtttt aactcctttt ccagcctaag ggtcttacgc
     7741 atgaagcggg ttgattgaag ccatgtaatg taggcaaatg cgaaaggaat taggtcggta
     7801 atccacagcg ttgggttggc agcatgttga cggaatatgt tttgaaggct aaaagtcagc
     7861 cccgatttgg agaaatcggc tatccatgca gcaaaagaaa agagtaggcc tacaagaaag
     7921 gctaacgcta tcttgtttcg ggttagattt tttgtttctc ttactgaggt taatctaaaa
     7981 gatgtcatgc tcctgcaaat attaattatt ttttgcggat tatatatttt ttatgtccaa
     8041 gcgctgtgtt taaggtaggt ttccggacgg ctgctccaaa gcaactctcc aagtttacgg
     8101 ccaagcctat caaaaagttc tacctgcatg ttgtcgaaag gtttaaaggc ggccaactca
     8161 aaaacgccga tggaattgtt gtcggggctt acaatgggag caatcagcag gtgtttgggc
     8221 gaaccctttc ctagtccgga gagtattgtg atgtagtttt cgggcacctg atcgaggtta
     8281 aggatttggc ggttttttgc cacctgtccg gaaagggttt cgccttccga ataggttacg
     8341 ggctcggatt cggaaaagta ggcatagcca gctttaaagg tgaaaaggtt ggtatcgccg
     8401 tcacgggtat agagtacggc ttgaactatt tcgtagtttc gggcgatgtt ggatagaagt
     8461 gcttccccaa atttttccag gttatcagta ggcagggtag gaataagacg tgacagaagt
     8521 ttatcataat caacagtttc ctcttggttt gtagcattcg cttgttcctg atttttaacc
     8581 tgttgaagga gcgcaaatgc tgcctttagc tccgctattt ctgcttcgag ttttgcggat
     8641 ttaacctgaa ggttgttgag catggcgtac agaccgatgc taataatggc ggggaggaat
     8701 agaagaacgt aacctatagc gggaaggctg cctcctgtgt tttggagtgt gttatggatt
     8761 aatccaaagg tgataactac accggcaata agcaaaacca gcaggagcaa tggaaggagt
     8821 tttgattttg agctaaaggc cttcatttct tcaagtttta atggtttgca aaaatctgat
     8881 tatttcgctg gtgttgtaca catggtcaac gggtgtcatt tttagggagg cctctgtcat
     8941 ggttctaacc tggcactcgt tggggtcctg aactattgcc agaccgccca gatcttttat
     9001 ttttttcagc ccgtaagcac catcccggtt agcccccgat aggataacac caatgagttt
     9061 atcgcggtac acctgggcgg cagttaaaaa tgaaaggtcg atgcttgggc gcgagtggtt
     9121 aacagggtcc tcagtggaaa gggctatttt attaccaagt tcaatgaaca tgtggtagtt
     9181 tgccggggcc aggtatgcct tgccgggctt tatctgttcc ttatcatcgg gctcaattat
     9241 gggaatacca gactttagcg agagcgcttc aacaaagccg ctgcggacgt gtttcagcct
     9301 atgtaggcac agaaggacgg gaaggggaaa agatgcaggt agggaatgca gtattcgggt
     9361 aattacctga aaactacctg ctgaaccgcc tataattact gccttataca tagtcattaa
     9421 acatttttgc gcttgtaaat tctttcggtt tcattaaata cggtaaattt attgtttgcg
     9481 tttgtatttt ccaaagtttc ttttattccc aaggcaagga acccaccagc caccaaactt
     9541 tctgcaattt tttgtaaaac ccgttcttgc agcagctggt tgtagtataa ggcttgattg
     9601 cggaaaagga caagttttac cgctccggga tccttgtcta ttatgggggt ttgcttaata
     9661 aagttcacat ctttaaccag tgtgctatcg aaaaagtact tgttcccctc tactttgtag
     9721 taggaggagt actgactttt accgttcagc ctaacgtagt tagcttcgtt tatttcaata
     9781 tccttagggt caagctggcc gctggtaatt ttctggatgg tggcattggt gaaaacgttg
     9841 ctgtaaatct gaaccttatc gagtgctccc atttccttca ggataatggt caatgagtaa
     9901 agctcctcgc cggaatcaaa cgaggcaatc caaattcggg gtttggtggt ggagttgcgg
     9961 atgatttcgg gtagaatatc gtcgcgtagg gttcgccaaa aggaaggatc gcgaaacatt
    10021 tcggtggtgt cgatattgac ctcatttagg aactggtcaa aaaaggttgg gttgcccttt
    10081 agcttgccaa ttagcccgtc ggcatccttt aggccgttgt ttgagattat ctgctcaagc
    10141 cttcgtttaa acgaggttag agcgtaatct ctaaagtcgt agccatagtc atccagaagc
    10201 gttttaataa cgttacgggt ttctactatg ccaatttcat actgcatcat tgtcaagcgt
    10261 catttcaaaa gtgaaaaatt aactctattc ctgtttaact tgttcatcat ttagagtgtg
    10321 ccaaattaaa agtgaattaa aaaatgttgt aatccagaga cgaatggcta actcgattaa
    10381 tcatgaatgt gttatgtcat aggtttttgg gtttgttctt catctgttca attatttttg
    10441 tcagctctgc ttccttattc ttgttttcgg ttatatcgaa cccgatgttg attatcttgt
    10501 aaagtttccc actttcgtcc attacggggg taaagttttc aagcacccat ttgacttccc
    10561 ccttgacggt aagggaaaac tcccgctgct gctttttccc attcaggatg ttgtcccaga
    10621 attttttata ctcagccggt cgatgcttgg ccgagtagtc aatgtcatgg aggaattttc
    10681 cctcgatatc ttccttctta tcgcccagca cctcgacgtt tttattgttg atgtaggtta
    10741 tcaatccgtc gggttcgata attgaaacca ggcagttttg gtctagcgcc tccagcaggg
    10801 tgctcatatc cttctgtcgc tgggccgata gctcctgttc cttctccagc tgtttcaggt
    10861 tttgttcaat ctcttcggct tggcgttggg ctttaatctc cagctgcttc atttcggtga
    10921 tgtcctgacc aataagcaga actttggtta tagctccagc ggggtccaac tcgggcgata
    10981 cggtaaccat aaggtagtgg ctgttgccgt ttcggttgag cacatgaata gtttctgtaa
    11041 tggtttgtcc agccctaact tttttccatg atatttgaaa atcggtgcgt tgttcggggt
    11101 taacgatgtc ggcaagcatt tttccttcaa gctcggtttt ggagtaccca agggtttcca
    11161 caaaattttg gttggagtct gctatgtagc cttcttcgga aagggtaaga gaaagtagcg
    11221 actggttaat ggtattggta atgagttcca actcaatgtt tttccgtagc atttcctcct
    11281 gggtggcctg catttcctcc atgttttggc gcatctcttc ctcttgtgcg gccatctcct
    11341 ccctttgctg ttgcgattgc tcgagaagca tgttggttcg tatgctgttt ttaacggcaa
    11401 agagggttga agcgatgctc tcgcctattt tttcaacaaa ctcaatttcg taaggtttaa
    11461 attccctgaa tgaggccacc tcaactactc caaaaatttc atcctcaagt ttaagtggaa
    11521 cgattagcag tgcggtaggg gtggcctctc ctaggccgga agttatggca atgtagtcag
    11581 caggtatctc cttgaggtag atggtctttt tctcaagagc gcaggcacca acaagtcctt
    11641 cgccaagggg gatggtttta ctgatgaatt ttttccggtt ataggcaaat gcagaaagca
    11701 tctcgaggtg cttgtcgttt tctgaatcgt tgtaaatgaa aatgcccccc tggtttgcct
    11761 cgaggtagtt gaccagattt tgaatgatgc tgtcggccaa tagggttagg ttgttgttgt
    11821 tttgacgcag gatatctcca aactttgcaa gaccttcgtt tacccagccc cggatgtagt
    11881 cttcctcctt tctgcgctgt tcttcctcca gggcatgctt caggtttgat tggatatcaa
    11941 gcagggcgtt gccaagttca tccttgctgc tcagaggttg gaaggtgtgc tccagctggt
    12001 tctgtccaat ttgatgtgca aagctggttg ttgactttag cccttccata agctggttta
    12061 gcgaggtaac catttcacca agctcatcct ttgatgttat tttgatgggg tttggaatgt
    12121 cgccttttgc cagctggcgg agctttatgt tcaccaggtt tataatgcgt atgatgggtt
    12181 taaggagtaa aggaattgca aaggctgtta gaagggacgc cagaatgata aatactataa
    12241 ttgccatgcg gttggatttc attggagcca aggcttcgtt catactgacc gagatggcaa
    12301 tgtaggcatt gtagggctta tagaacttgt agcatagcaa ccgcttctcg ggtttgtttc
    12361 ctgaataatc gtcataatat atgtagccgt cggtgctttt gctctttcta atctgggcag
    12421 ttgcagcaga gggaagtctt tgcccttgaa gtgttgtgtg aagcaggtaa acgccattgg
    12481 ttgagataat ggcggggtaa tcggtactat agtaggctgg cgagaggaag taagtcctga
    12541 gctcgaaaat atcatcggag gataggccgc ttcctgtttt tgcttccacc atatcgacaa
    12601 tttgtatcag cttgtctatg tacactcctt cctgaattcg tatgctttcc ctaaccctat
    12661 tttttgacag gtagttcagc gttactccgg aagcggaaaa caacaggatc atcagtggaa
    12721 cgataatgag ctgaatacgt tgtgaaattt tcatttcatg tagaatttaa agttacaatt
    12781 cgttacattt ttccctaaaa gcttacttaa cgtgatgata agttttccac cagcttgcct
    12841 ctgaccaaaa aaaagtattc tacatagtac gttgccaatt gtgcaatcag cggtgttgaa
    12901 attgtcggta tcaaaaggtt gcttcataac tattttttca catagctttg tccaagtttt
    12961 tcaaacttag tggcccaggg gccaagtatg gtctcgtgca ttccaagcag cagcactccc
    13021 ttggggtaaa ggtttgagtg aaatagttca aataccttgt tctgaaggga gtagttgaag
    13081 tagataatca catttctgca aaggatgagg tcgaacttgg caaagaaaat attcccgtcg
    13141 gttaccaggt cgtgcttacg gaaaattggt ttttccgaca ggaatttgtt catggttatg
    13201 gtatccttta ccttgtcaat ttcaaagtac ttttcgtagg gaacctcgtt gtactcttca
    13261 aagttcaagg ggttttgctt gatcaccttg tcgaagttgt ccaggtagcc caggttgaaa
    13321 cggtacttgt aaaccccctg ttttgcagca tccagcacgt caatatttat atcggtggca
    13381 aaaattttag cctgttccaa catacccatt tcgttgagca gtatcatcat ggagtaaacc
    13441 tcttgcccgg tggagcaacc agcatgccaa atgtttatgg ttttgttgtt tctaaagcgc
    13501 gggagtattc gggagcgtaa gaggtgccac accgggggat cgcgaaaaag ctctgtggta
    13561 ttcacggtaa cttcctttac cactttttca atgaatgccc tgtcgttttt aaggttggcg
    13621 attaggctga atatatccag gttgtaatcc tccagaattt tctgcaagcg ccgcttcagc
    13681 gatttatccg agtactcgga aaaatcgtac tttgatacat ttttaagcgt agtgataaag
    13741 tattgcaaat cctgatcggt tacctgcatg tgcttcaact taataactcc atttttggta
    13801 aaaacgtttt agtacgcctg ccaaactgca tccctttttc cctgttaaag gtaagtatag
    13861 tttgtaaatt gacaaagcgt cgtgaaagtt atagcgattt gaataaattc caaaaatacg
    13921 aagaagaggg gaaacagcta ctttgacaaa cgactgattt ttaaatttca tgttgccata
    13981 cctgttgacc aaggcaacat ggagttatag gatttgttag aattttggtt tgaattcttg
    14041 atagctgttt gtgataagga gtccaactaa ttacttaacc ggttagttgc tgtttttgat
    14101 taacgtcaag cccgtgcggc tgcatttggt taatgtatta tggattgcct attaacctaa
    14161 actcaagggt taccgggtta tgatcggaat tcttgaaagc catgttggct gtctggcagc
    14221 gctgaacact cacgttgggt gaaaccaaaa agaaatcgag aatacacgta ttggtgcggc
    14281 ctggagtgta gggttcgttt aagtaccggt tggtggggca cgaagggtca aatacccatg
    14341 accagtcttt aggcataaag ctactgtcta taccggttag catgaacgaa gagttgctgc
    14401 tacctacaac aagttcagca tatggtgctc caggcggaag ctggttccaa tcgccaccgg
    14461 ccaccacgta gttgcccttg tagtactcgc ttagtaaaat ttcttgcagg aaatccattt
    14521 cctgttgttt aagtgcgcca ttgtcgaatg cggagttatg agtattgacg agaacaaaat
    14581 ccttaccatt gcttgtgggg tagcggttca ctaccatgca acgccgcagg ttgaagagcc
    14641 tgtttggcca ggggaatgcc ccaggatacg cataccttat cgtttcctta gggttaaact
    14701 tgctgaaggt tacaatgccc gactgcacct ttcccatagg gtttttaagg ggtacgggaa
    14761 caaaattaac ccgatagtta atgcctatag agtacgggta ttctaaagtt cttgaaattt
    14821 cctccagctc gtttattccg tacgagcgat gggagttaat atcaacttcc tgaagcagga
    14881 tgaagtcgtt gattttttca ccgcctaggg ttttctcaat tgcggttaaa ttctcggttg
    14941 ttcgttcacg cgtatcccgc atttttcccc ctccatccat aaagaagtcc atgttgctgc
    15001 ctaatcctgc atagcctata ttccaagtta gtgccgccag ggtggaatcg aatggaattg
    15061 tatcgggctt gtccgactgc caaagcacaa ccctactttc tggatcgaag taggtgattg
    15121 aggagtagag gaggaagaaa acaagaaaaa ttatgagaaa actccctact ttcccgattg
    15181 tttttagtat tctaccaaac attttaagg
//
LOCUS       DAOPHX010000057        14964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_4583_length_14964_cov_6.254678, whole genome shotgun sequence.
ACCESSION   DAOPHX010000057 DAOPHX010000000
VERSION     DAOPHX010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 14964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14964
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4583_length_14964_cov_6.254678"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..5512
                     /locus_tag="PK502_05755"
     CDS             128..5512
                     /locus_tag="PK502_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPH00354.1"
                     /translation="MRHGYNPSSSNSWWIYLMTGDEIATGTPASGYAVGVNLSGSDDM
                     LKVWRVDNGIPQALLSSTLNWETTIGTANVGAIEVKRLSNGTFNLKASAGGTFDNLND
                     YGSAVDVTYAAFKYLSIQYRYTSSADLKLWVDNIGVDYQAANPNDTTTTIYQPMLQIP
                     SGEILSTSTTPSKAVDVLRFVVEDMGSGDGLPTYVKRVAFTNPIPEISQWNSIIAGVL
                     LKGANGEIPIKSSFISNTKLTLEVDSSEMIIPDGLAGEYTLSIFLKSSGITDGTSLQF
                     KIDSIDHEFLASVAGSGFPKVLPHLITSGLFTIRVDATMLKIHEYSHNYIIGKPFALG
                     VAGCDSLGNVDTNFNHDIALNLVQGSGQLSSLLGLVQPAIGGIAVWNDLIYNKNENIR
                     IEATSQGLSPALSSEIFISFDTTSYAGNPSFQPEAEDILSTKNSAADAVEVMRIKIFD
                     VGGDSASTLLKSLRLTRANLPNMAALNKVLGGILVKSGSTWCGLSDAQVKTSTIDIYF
                     SNGSVCVPEGGNLEIGIWVYLKPTGITDNQTITLYIDPLNPLLTAYDNGTQFSSTFPS
                     GIYSSTFTIRVHATHLKFDQTPARVGVNEAFPVVVKAADINGNTDTDYFGSLGLGLAA
                     GDGHLDVSGGTEGSLQNGIASYIASYSKPGSFALLAMEPTLEDVASPFITCGDADGQV
                     YPLFTGSDTLIFTAQNTNTSAALEIFRLKVSDSGTSDGLPLKVKTICLTAFNPNSASS
                     LEKIIGGFMLRLEEKVIIPIEFSQSEGVFYIQLSENQLEIADGDTIEMGVELYLNPAF
                     LKDNEKFQFYIPSSSHGWVAYDDGTAFCPNFLSYIYGLPCSIEVEADRLAFNSYPFAA
                     TPNDCFDVEVISTDCFGNPDANQLGQVTLSENSASGNIEIPDSTEPLSDGIATWSNIK
                     FLNEGRFTLKASHDTLLYAVTPQIFCGYSQMCLIEEAFEGDIPGWGGVESWRVSTISP
                     VAGGSSLVHAGDPGAEKSELVIPLNANAYGRVVEWNVVVKTGSWDPSTDNYFYMVLGS
                     TDSLFNNATTSGYAVGINPSEVDDFLSIWHFEGGKKTNLITTRFDWSEKDEVEIRVTL
                     SPTGILSLWYKPKDYGRMVNGGEVLIQEAIPMKYAGFVYAYTPTRAGQLWLDNLKLCL
                     TEFPPLVKSVRLSSLNSVKVSFSKNVSITSASIPQNYLVRTAEGEPVAVKSVSIDQSK
                     HDMAVLTTEKLSFGSYTLYISNIEDEQGHFTSDSATFGLGSNGNFGRLVINEIMANPI
                     PSNGLPEYEYIELYNPSADTVFTKGWRIVLNDLQLNLPSDTVLPRTFTLIGGSTAMSE
                     MAVFGKTMGVTSFPSLLNDGMLIKLIDGEGNLISFASYSSDWYNDDSRSDGGYSLECI
                     DASNIAEGKRNWHACMDELGGTPCKENSVVAINPDITPPKVIYLEVLSEDTIRIAFSE
                     PMDSLTITDIENYSLSSENLGVKKIMLQGNLYDGVKLILNNPMEPGSFYQLAIGGEIV
                     DFSGNPIEESTFKIGLPQIPAAGDLVVNEVLFNPYSGGVDFVEIFNNSQKCIDLKNLL
                     LANRNTDLSLNGVNPAADTSRLIFPAEYAVITVNPQQVKQFYYCENEKAFVQVSSMAS
                     LNSDEGYVVLLNANMDVVDELHYTEKLHNPIITNVKGVSLERINPKLPSADASTWHSA
                     AQSVGFATPTFKNSQYAETSMEDGYFTISPEIISPDGDGKDDFLQLCYSLPAEGYVSN
                     IRIFTSAGVEVFRLANNLLLGREGCLRWDGVNYANRIVPTGIYVVYVEYFNLSGEVKR
                     EKRVCVVAER"
     gene            complement(5574..6317)
                     /locus_tag="PK502_05760"
     CDS             complement(5574..6317)
                     /locus_tag="PK502_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00355.1"
                     /translation="MKKILLPFPIIILVILTSTLMAKAQQTVVFQASDGLEVTADLYV
                     KGTDLPYIILLHQARYSRGEYREIAPKLVNLGYNCLAVDLRSGGEVNGVVNRTAMLAA
                     GKRIPAEYYNAMSDVEGAIGYVKSKSDKPIVLWGSSYSASLALIAGAKNLRVAAIVAF
                     SPGEYFDQPDFVSSKIGKLAIPVLTLSSKAECQPVRELLSGVNPKLVSYYCPTDSGQH
                     GSKALWQSNPSNGDYWMAITMFFSRLNKN"
     gene            complement(6670..6930)
                     /gene="atpC"
                     /locus_tag="PK502_05765"
     CDS             complement(6670..6930)
                     /gene="atpC"
                     /locus_tag="PK502_05765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HPH00356.1"
                     /translation="MVCDLISPERNIKVENVNYVKVPGTKGSFGILKKHAPIVSSLEP
                     GAVKVILDDKTKLLFAIPSGGFVEVTDDRVTIIAEEVLQQPE"
     gene            complement(6989..8500)
                     /gene="atpD"
                     /locus_tag="PK502_05770"
     CDS             complement(6989..8500)
                     /gene="atpD"
                     /locus_tag="PK502_05770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPH00357.1"
                     /translation="MAELAGEIVQIIGPVVDVSFENSNGKLPSINEALELRRTDHSVL
                     VLECQQHIGENTIRAVAMDSTDGLSRGMKVKATGKSITMPVGEQVRGRLLNVIGDSID
                     GLSTLNREADYQIHSAPPKFDELRPEKEVLFTGIKVIDLLEPYSKGGKIGLFGGAGVG
                     KTVIIMELINNIAKGYSGMSVFAGVGERTREGNDLLREMIESDVIRYGEEFKQTMKEG
                     GWDLTKVDTDELRKSQATLIFGQMNEPPGARATVALSGLTVAESFRDGGSGEKGNDIL
                     FFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLSTEMGLMQERITSTKRGSITSVQAI
                     YVPADDLTDPAPATTFAHLDATTVLDRKIAELGIYPAVNPLDSTSRILTPDVVGEEHY
                     NTAQRVKNLLQRYQELQDIIAILGMDELSEEDKLIVHRARRVQRFLSQPFHVAEAFTG
                     TKGVMVNINDTIKGFNMIMDGEVDKYPEAAFNLVGTIEEAIEKGERLLKEAKA"
     gene            complement(8586..11645)
                     /locus_tag="PK502_05775"
     CDS             complement(8586..11645)
                     /locus_tag="PK502_05775"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HPH00358.1"
                     /translation="MGKFKKVNNITGWIVFAISAVVYLLTIEPTASFWDCGEFIATAF
                     KQEVGHPPGAPLFMMIARFFTLFASDVTKVAALVNAMSALASAFTILFLFWTITHLAR
                     KLIIKDAENISTGNLVAVMASGVVGALAYTFSDTFWFSAVEGEVYAMSSFFTAIVFWA
                     MLKWEEVADEPHSNRWLIFIAYMMGLSIGVHLLNLLTIPALAFIYYYKKYKPTRNGIF
                     VALLVSAVILVAILYIIIPGTFKLGSLFELMFVNGFGLPYFSGLIFYFILLFTLIAWG
                     IYNSHKKKKVLLNTVLVSVAAILIGYSSYGMIILRANANTPINENNPSNVFSLINYLN
                     REQYGDRPLFYGQYYNAPVTGSKDKTTRIPKNGKYVETYLKTTYNFDDQFKTIFPRMY
                     SPQGDHVEEYKKWANIEGRPIKVRNQNGEEEIRNVPTFGENLLFFFRYQVGHMYMRYF
                     MWNFVGRQDDVQSNGGIEHGNWISGIKPIDAIFLGNQNELTDNMKNHPSRNTYYFLPL
                     ILGLIGIYYQLFTKRDRRNSFVVFLLFFLTGLAIVVYLNQTPLQPRERDYAYAGSFYA
                     FTIWIGLGVLAVYEWIQKIAPTTASAAAAGMVCLSVPIIMGTQNWDDHDRSGRYTARD
                     FAANYLNSCAPNAILFTNGDNDTFPLWYAQEVEGIRTDVRVVNLSLLGTDWYIDQMKC
                     QCYDSKPLPISMKHEQYVQGTRDIVYIYNRLNDTVELKNLLNFVLSDDKSTKLQIPGE
                     SPIDYIPASRVKVSVNKAAVIKNGTVNLKDSSLIVSSMAWSLKGQYLQKSALAILDII
                     TNNNWERPIYFVSPYGDGDIGIADYLQLEGFAYRLVPIKTSGRDFLSVGRIDTDILYN
                     NLMNKFSWGRMNEPDVFIDYNNQRTAIVLKLRNTFSRLAQQLISEGKKDSALVVLDKI
                     TKLMPHEKYPYDFFMFDIAEAYYKLNQTETANKILDKYADVTLQSLRYQLSLGDKYRD
                     YNNYDLRLNIQTLQELASLADSYGQVELKTKIENGLNQHYSKLVNVK"
     gene            11910..12257
                     /locus_tag="PK502_05780"
     CDS             11910..12257
                     /locus_tag="PK502_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HPH00359.1"
                     /translation="MKREEQDNDKLKFAYSTNPDFKASNEMQPEQESISPEKQKLVVC
                     IERKNRGGKCVTLVKGFRGSNDDLDTLGKQLKSRCGVGGSVKDGEIIIQGELRQKVAA
                     ILRSYGYNVVVSG"
     gene            12311..13531
                     /locus_tag="PK502_05785"
     CDS             12311..13531
                     /locus_tag="PK502_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00360.1"
                     /translation="MILSFGAAADTLTVMHYNLMYYDREYEECTAANNNVDSKDEYLR
                     TIIGYARPDILTVNEVNASVSSVERIRTNVLNINGITKYKRANFSGSFLANMIYFNSE
                     KVELKSQDVIPTSPRETNVYKLYLKTSSLVQGDTIFLYCMITHLKAGSDPSDEDDRTA
                     ASAAIMSYLASSNLNGNVLLMGDMNLYSAEEGAFVNFTTPTGINQFRFYDPLSMVGDW
                     HNNYSYRNIHTQSTHTTSDGCHSTGGMDDRFDFILSSSSLTTGNDGLKITSYKTIAQD
                     GNRFNESLINPANYSVPTDVLSALYNNSDHLPLKMSLYSDFSLHSATKSNLVPKLVFN
                     NPANNKLHVHVPEGISIVTLNLYSITGGKMLSYQGAEPVVDMDISGLSLGIYIAEATL
                     SDGSVSFFKVLKIR"
     gene            13566..>14964
                     /locus_tag="PK502_05790"
     CDS             13566..>14964
                     /locus_tag="PK502_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00361.1"
                     /translation="MRRLIIILSTIFLIQTAAKAQYYQTGVEPGYTKWRQIESGKIRV
                     IYPSEYEDKASSYLNLLLTTDSLTGRDYNLKSKRIDVVLHPNSMLSNGFVAWAPRRME
                     LITFPSTDGYAQPWSSQLALHEMRHVKQMYALNSSTSRLAYYLFGEQVVGLAAGLVPQ
                     WFLEGDAVVTETAHSFSGRGRSATFWQHYRAHFLADRKTYSYNKWLLGSYKNYIPNSY
                     SLGYQLVSFGNFKYGINTWPKVVNHVAKYPFTAIPFYFGVKQNTGLPARKLADSTFAY
                     LIGRWKADTVDRYLSAVNHPVRMPKSYTSYLYPYKLNDSTMLAYRTSLNDIPAFVEIN
                     LNSQKVKELCYPGYLLGRPTYSDSLIVWAEYQPHTRWEYEDFGRIYGLRIKDVQKIEL
                     SGKGKYFSPAINPDDCSVLCISYSPDGNFKLVSIGKNREERELLSFSSGYEVQELSFD
                     RNTRMLYALVVTQQGK"
BASE COUNT         4253 a         3189 c         3340 g         4182 t
ORIGIN      
        1 ccccgatagg ttaacaccaa catcatagcc tgaagcagga gtgcctgttg caatttcatc
       61 tcccgtcatc atggtcagtc ttccaagctg gaatgttaac aatggagatg taacgtggag
      121 gatgcttgtg cggcatggtt ataatccctc ctcctctaac agctggtgga tttacctgat
      181 gacgggagat gaaattgcaa caggcactcc tgcttcaggc tatgctgttg gtgttaacct
      241 atcggggagt gatgacatgc taaaggtgtg gagggttgat aacggcattc cccaggcgct
      301 gttgtcatct accttaaatt gggaaacaac aataggaacg gcaaatgttg gggctataga
      361 ggtaaagagg ctttccaacg gaacatttaa ccttaaagcc tcggcgggcg gaaccttcga
      421 caatcttaac gattacggta gcgctgtaga tgttacgtat gccgcattta aatatttgtc
      481 aatccagtac aggtacacca gctctgccga tttgaaactt tgggttgaca atatcggcgt
      541 tgactatcag gcagcaaatc ctaatgatac caccaccacc atttatcagc caatgctgca
      601 aataccgtct ggagaaattt tatcaacctc aaccacacca tccaaggctg ttgatgtgct
      661 gaggtttgtt gtggaggaca tgggttcagg tgatggattg cccacgtatg taaagcgtgt
      721 agcctttacc aatcctattc cggaaatttc acaatggaac agcataatag caggagtcct
      781 gctaaaagga gctaacggtg aaattccaat taagtcgtcg tttatttcaa ataccaagct
      841 aacgctagaa gtagattcat ctgaaatgat cattcccgac gggctagccg gagagtacac
      901 acttagcatt tttttaaaat cctctgggat aaccgatggc accagcttac agtttaaaat
      961 tgacagcatt gaccacgaat ttctggcaag tgttgctggt tcaggatttc cgaaggtttt
     1021 accccacttg attacatccg gtctattcac cattagggtg gatgctacca tgttgaaaat
     1081 ccacgaatat tcccataatt atattattgg caagccattc gcactcgggg ttgccggatg
     1141 cgattcactg ggaaatgtgg atacgaattt taaccacgat attgccctta accttgttca
     1201 gggaagtgga cagctttctt cattgctggg tttggtgcaa cctgcaatag gaggtatcgc
     1261 tgtttggaac gatttgattt acaacaaaaa cgagaatata aggattgagg caacaagcca
     1321 gggtttgagc ccggcgttaa gcagtgaaat ttttatttca tttgatacaa cctcatatgc
     1381 tggaaatcct tcttttcaac ccgaagcaga agacatcttg tccactaaga attccgccgc
     1441 cgatgctgtt gaagttatgc gtataaagat attcgacgta ggaggtgata gcgcttccac
     1501 cttactcaaa tcgttaaggc taacacgtgc caaccttcca aacatggctg ctttaaacaa
     1561 ggtgttaggt ggaatcttag taaagtctgg ttctacttgg tgtgggcttt cggatgctca
     1621 ggtcaaaaca tcaaccattg acatctattt ttctaatggc tctgtatgcg tcccggaggg
     1681 gggaaatctg gaaatcggca tttgggtata tctaaaacca accggcatta ccgataacca
     1741 aacaatcacg ctgtatatag acccattaaa tccgctttta actgcatacg acaacggtac
     1801 acagttttct tctacttttc cctctggaat atactcgagc acctttacca taagagtcca
     1861 tgctacccac ctaaaatttg accaaactcc agcaagagtt ggtgttaacg aggcttttcc
     1921 tgtggttgta aaggctgctg atataaatgg caatactgat accgattatt ttggtagctt
     1981 aggtctaggt ctagcagcag gagacggcca tctcgatgtt agtggaggta ctgagggcag
     2041 ccttcaaaac gggatagcca gctatatagc cagctactcc aaacctggta gctttgcgct
     2101 gcttgccatg gagccaacac ttgaggacgt ggcatcaccc ttcataactt gtggcgacgc
     2161 cgacggacag gtttatccgc tttttaccgg tagcgacacg ttaatattta cagcacaaaa
     2221 taccaatacc agcgctgccc ttgaaatatt tcgcctaaaa gtttccgatt ctggtacttc
     2281 agatgggtta cccctaaaag taaaaacaat ttgccttact gcatttaacc caaatagcgc
     2341 ttcatcttta gaaaaaatta ttggtggttt catgctgaga cttgaagaaa aggtgattat
     2401 tccaatagaa ttcagccaat ctgaaggagt tttttacatt cagctcagcg aaaaccagct
     2461 cgaaattgct gatggcgaca ctatcgaaat gggagtagag ctgtacctta atcccgcctt
     2521 tttaaaggat aacgagaaat tccaatttta tattccgtca tcctcacacg ggtgggtagc
     2581 ttacgatgat ggaacggcat tttgtcccaa tttcctgtcg tatatttatg ggcttccctg
     2641 tagcatagag gtcgaagccg acaggcttgc ttttaacagc tatccatttg ctgctacccc
     2701 aaacgactgt tttgatgtgg aagttatatc aaccgattgt tttggtaatc cggacgccaa
     2761 ccagctgggt caagtaaccc tatccgaaaa tagcgcaagt ggcaacattg aaatccccga
     2821 ttcaacagaa cctttaagcg atggaattgc tacttggagc aacataaaat ttctaaatga
     2881 aggaagattt acccttaaag catctcacga tacattgctt tatgccgtaa cgccccaaat
     2941 tttttgtggg tattcacaaa tgtgccttat tgaagaggct tttgagggtg atatacccgg
     3001 ttggggcggt gttgaaagct ggcgggtttc aaccatttcg ccggtagctg gcggcagttc
     3061 actggtacat gcgggtgatc ctggtgcaga aaaatccgag ttggttattc ctctgaatgc
     3121 caatgcttat ggcagagtgg ttgagtggaa tgttgtggta aaaacaggca gctgggaccc
     3181 ttcaaccgat aactactttt acatggtact gggatcaacc gatagtttgt ttaacaacgc
     3241 cacaacatct ggttacgctg ttggcatcaa cccttcggaa gttgacgatt ttctatcaat
     3301 atggcatttc gaagggggaa agaaaacaaa cctgattaca acacgatttg actggagcga
     3361 aaaagatgag gttgaaattc gtgtaaccct ttcgcctacc ggaatccttt cgctttggta
     3421 caaacccaag gattatggta gaatggtaaa tggtggagaa gtgcttatcc aggaagctat
     3481 tccaatgaaa tatgcgggat ttgtttatgc atatacaccc acaagggcag gacagctgtg
     3541 gttggataat ctaaaacttt gcctaacgga gtttccccct ttagtgaaat ctgtaaggct
     3601 atccagccta aactcggtga aggtttcttt tagtaaaaat gtatccatta ccagcgcttc
     3661 aatccctcaa aactacctgg ttagaactgc cgaaggggaa ccggttgctg taaaatcggt
     3721 gtctattgat cagtcgaaac acgacatggc agtacttaca accgaaaaat tgtcctttgg
     3781 tagctatacg ctttatataa gcaacatcga ggatgaacaa ggccatttta cttccgatag
     3841 cgcaacgttt ggtttaggct cgaatgggaa ttttggacgc ttggttatca acgaaatcat
     3901 ggctaaccca attccctcga atggattacc agagtacgag tacattgagc tctacaaccc
     3961 ttcagccgac acagttttca ccaaggggtg gcgaattgtc cttaatgatc tccagctaaa
     4021 ccttccctcg gatactgttt taccccgaac ttttaccttg attggtgggt caacagccat
     4081 gagtgaaatg gcagtatttg gcaaaactat gggtgtaacc tcttttccct cgctgctcaa
     4141 cgatggtatg ctgataaaac ttattgatgg cgaaggcaac ctgatttctt ttgcaagcta
     4201 tagcagtgac tggtataatg atgatagtag gagtgatggg ggctactcgc tcgagtgcat
     4261 cgatgcctct aacattgccg aaggcaaaag aaattggcat gcctgcatgg atgaactagg
     4321 tgggacaccc tgcaaggaaa actccgtagt ggccattaat cccgatatta ctcctccaaa
     4381 ggttatctat ctggaagtgc tttcggagga cacaattaga attgccttta gcgagccgat
     4441 ggatagcctt accattaccg atattgaaaa ctattcgtta agttctgaaa acttaggcgt
     4501 gaaaaagatt atgctgcaag gaaatcttta cgatggtgtt aagcttattc taaacaaccc
     4561 catggagcca ggcagctttt accagttagc tattggaggt gaaattgttg atttttcggg
     4621 aaaccctatt gaagaaagta cttttaaaat tggattacct caaattccgg ccgcaggaga
     4681 ccttgtggta aacgaggttc tgtttaatcc ctatagcgga ggagttgact ttgttgaaat
     4741 tttcaataat tctcaaaagt gcatcgattt aaaaaaccta cttttagcta acaggaatac
     4801 agatttatcg ctaaatgggg taaatcccgc agccgatacc tccaggctga tttttcctgc
     4861 tgaatatgcg gttataactg ttaaccctca acaggttaag cagttctact attgtgagaa
     4921 tgaaaaggcc tttgtgcagg tttcatctat ggcttcgctg aatagtgatg agggctatgt
     4981 ggtgttgctc aatgctaaca tggatgtggt tgacgagctg cactataccg aaaaattgca
     5041 taacccaatt ataaccaacg tcaaaggggt ttctcttgaa cggattaacc cgaagttgcc
     5101 ctctgccgat gcaagtactt ggcattcggc agcacaaagc gtaggatttg ccacacctac
     5161 atttaaaaat tcgcagtatg ctgaaacttc aatggaagat ggctatttta ccatttcgcc
     5221 ggaaattatc tcgccagatg gggatgggaa ggacgatttt cttcagcttt gctatagtct
     5281 gccggctgag ggatacgttt caaacatccg catattcacc tctgccggag tagaagtttt
     5341 tcgcctggca aataacctcc ttttaggaag ggagggatgc cttagatggg atggcgtaaa
     5401 ctatgcaaac agaattgttc caacaggaat atatgttgtg tatgttgagt acttcaacct
     5461 atctggtgaa gttaaacggg aaaaaagagt gtgcgtggtg gccgaacgat agcttcaaaa
     5521 gcatctctgc cggtatggct tttccattct gacttttatc cggtaaacaa gaattaattc
     5581 ttgtttagcc ggctgaagaa catggtgata gccatccagt aatcaccgtt ggaggggttt
     5641 gattgccaaa gagccttaga cccatgctgt cccgaatcgg tagggcaata ataagaaaca
     5701 agttttggat ttacgcctga gagcagctct ctgactggtt ggcattcggc tttagacgaa
     5761 agagttaaaa caggaatagc gagtttgccg atcttgctac taacaaaatc aggctggtca
     5821 aaatactcac ccggtgaaaa agccacaata gctgcaaccc ttaagttctt tgcaccggct
     5881 atcaatgcta gcgaggctga gtatgaactt ccccaaagca cgataggctt gtcggacttt
     5941 gacttaacgt agccaattgc cccttctaca tcgctcatgg cattataata ttcggcaggt
     6001 attcgtttac cagcagccag catggctgtc ctatttacga ccccgtttac ctctccacct
     6061 gaacgaaggt caaccgccag gcagttgtag cccaagttaa ccagttttgg agcaatttct
     6121 cggtattcac ccctgctgta ccttgcctgg tggagcaaga tgatataggg caagtcggta
     6181 cccttgacgt aaaggtcggc agtaacctct agtccatcgc tggcttgaaa aacgacagtt
     6241 tgttgtgctt ttgccataag ggttgacgtt aatattacca gtatgatgat cgggaatggt
     6301 agtaaaatct ttttcatgcc tttaggtttg cttggctgta aggaataatt gtgggtataa
     6361 ggcgaaaata ttaacgttag agtaaccgga taaatttggt aagtatctaa aatgatagta
     6421 tgcctgcaga gttgaacact taaaccaaaa tgatggaaat ttattcacct tcaggtggag
     6481 cgaaaaagct actattatca gatggcaatt gttgattaat tgtgcctctt tttctacctt
     6541 tcagcgtaaa ctaccagctt aaagcctaac tttattaaat agtaggtcag agggggcaaa
     6601 agcaaaaata cagaaacaac tccttatatt gcaaagtagg gcaagccagc aactggtgct
     6661 taagaaaggc tattcgggtt gctgcagcac ctcttcggca atgattgtaa cccgatcgtc
     6721 agttacttca acaaaaccac ctgaaggtat tgcaaaaaga agtttagttt tatcgtcaag
     6781 gataacttta acagctccgg gttccagtga tgaaacaatt ggagcatgct tttttagaat
     6841 gccaaacgaa ccctttgtcc caggtacctt gacgtagttc acattttcaa ccttgatgtt
     6901 cctctcgggt gaaatcaagt cgcaaaccat ttgcccttct atattagttc aggtctaaaa
     6961 tactctctta ttgtagtcgg taaaggtgtt acgctttagc ctctttcaac aggcgctctc
     7021 ctttctcaat agcctcttca atggtaccaa caaggttaaa agccgcttca gggtacttat
     7081 caacctcacc atccataatc atgttaaatc ccttgatggt gtcgttaatg tttaccataa
     7141 cgccctttgt tcctgtaaaa gcttcggcaa catgaaatgg ttgggagagg aacctttgaa
     7201 ccctacgggc tcggtgcaca ataagtttat cctcctccga cagctcatcc atacccaaga
     7261 ttgcaataat atcctgtagc tcctgatagc gctgaagaag attttttacc cgttgcgcag
     7321 tgttgtagtg ttcttctccc acaacatcgg gggtaagtat tctcgaggtt gagtcaagag
     7381 ggttcacggc ggggtaaatt cccagctcgg ctattttgcg gtctaaaacg gtagtagcat
     7441 caaggtgagc aaaggtagtc gcaggagcag ggtcggtaag gtcgtctgca ggaacatata
     7501 tagcctgcac cgaggtgata gaacctcgtt ttgttgaggt aattctttcc tgcattaacc
     7561 ccatctcggt tgatagtgtt ggttggtagc ctacagccga gggcatacgc cccagcaagg
     7621 cagaaacttc agatccggcc tgggtaaaac ggaagatatt atcaataaag aagagaatat
     7681 cgttaccttt ttcaccactt cccccatcac ggaacgattc agcaacagta aggcctgaaa
     7741 gagcaacggt tgcacgagca ccgggtggtt cgttcatttg accaaaaatg agggtagctt
     7801 gtgattttct tagctcatcg gtgtcaacct ttgtgaggtc ccatccaccc tccttcatgg
     7861 tttgtttaaa ttcctcgccg taacgtatta catcggattc aatcatttcg cgcaagaggt
     7921 catttccttc gcgggtgcgc tctccaacgc cagcaaaaac agacattccg ctataccctt
     7981 tggcaatatt gttaataagc tccatgatga tgacggtttt tcctacacct gcaccaccaa
     8041 atagtccaat cttaccaccc ttagagtagg gttcaagtag gtcaattacc ttaattccag
     8101 taaaaaggac ttctttttct ggacggagct catcaaattt aggtggagca ctgtggattt
     8161 ggtagtctgc ctcacggttc aacgttgaaa ggccatcaat ggaatcccca ataacattaa
     8221 gtaaacgacc cctaacctgt tcaccaacgg gcattgttat agactttccg gttgctttaa
     8281 ccttcattcc acgtgaaagt ccatcggtgg agtccatggc aacagcacgg atggtgtttt
     8341 ctcctatatg ctgttggcat tcaagaacta aaacagaatg atcggttctc cttaattcta
     8401 acgcttcatt gattgaaggc aacttcccgt tgctgttttc aaatgaaaca tcaacaacag
     8461 gcccaataat ctgaacaatt tcaccagcta gttctgccat atgatgaatt tttcttaaag
     8521 ctcaaaggta tcaacaaaaa taattgatta aaccaaaacg atgatttttt atttcagcgt
     8581 actatttatt tgacattaac aagcttacta tagtgctgat ttaaaccgtt ttctatcttg
     8641 gtcttcagct caacctgccc gtagctatcg gcaagggaag caagttcctg cagggtttga
     8701 atgttaagac ggagatcgta attgttgtaa tccctgtact tatcgcccag gcttaactgg
     8761 tagcgaagcg attgaagggt aacatcggcg tacttgtcca atatcttgtt ggctgtttcg
     8821 gtttggttga gcttgtagta tgcttcggca atgtcgaaca tgaagaagtc gtagggatat
     8881 ttttcgtgag gcataagctt ggttatctta tcaagaacaa ccagagcaga gtcctttttc
     8941 ccctcggaaa ttagctgttg agcaagcctg ctgaatgtgt tgcggagctt tagaacgatt
     9001 gccgtacgtt ggttgttgta atcaatgaaa acatcaggtt cgttcattct tccccacgaa
     9061 aacttgttca tcaggttgtt gtagagaata tcggtatcta ttcttcctac agaaagaaaa
     9121 tccctaccgc ttgttttaat aggaaccagc ctgtaggcaa aaccctcaag ctgaaggtaa
     9181 tcggcaattc ctatatcccc atcgccgtaa ggcgaaacga agtaaatcgg acgctcccag
     9241 ttattgttgg taatgatatc cagaatggcc agcgcgcttt tctgaaggta ctgaccttta
     9301 agagaccatg ccatggacga cacaataagc gaagaatcct tcaggtttac agtgccgttt
     9361 ttaatgaccg cagccttatt tacagaaact ttaaccctac tggctggaat atagtcaatt
     9421 ggcgattcgc cagggatttg gagcttagtg gacttatcgt cgctcagcac aaagtttaac
     9481 aggtttttaa gttcaaccgt atcgttcaga cggttataga tgtatacaat atcgcgggtt
     9541 ccctgaacat actgttcatg tttcatcgaa attggcagtg gtttggagtc gtaacattgg
     9601 cacttcattt ggtcaatata ccagtcggtt ccaagcaggc tgaggttaac cacccgtaca
     9661 tccgttcgta ttccctctac ttcctgcgca taccaaaggg gaaatgtgtc attatcgcca
     9721 ttggtgaata gaatggcgtt tggtgcgcaa gaattgagat agtttgcagc gaagtccctt
     9781 gcggtgtaac gtccagaacg gtcgtggtcg tcccaatttt gagttcccat gataattggc
     9841 acgctaagac aaaccattcc tgctgcagca gcagatgcgg tagtgggagc aattttttga
     9901 atccattcgt aaactgccag caccccaagc ccaatccata ttgtgaaagc ataaaatgac
     9961 ccggcatagg cgtagtcacg ttcgcggggt tgcaacgggg tttggtttaa gtaaacaact
    10021 atggctaacc cggtgagaaa aaacagaagg aaaacaacaa aggagttgcg cctatcgcgt
    10081 ttggtaaata gttggtagta aatacctatg aggccgagaa ttagggggag aaaatagtag
    10141 gtattgcgag aggggtggtt cttcatgtta tcggttagct cgttctggtt acccaggaag
    10201 atggcgtcaa ttggtttaat tccgctaatc cagttcccat gctcaattcc gccattgctc
    10261 tgaacatcgt cctgacggcc cacgaaattc cacataaagt agcgcatgta catgtgccct
    10321 acttggtagc ggaaaaagaa caggagattt tcaccgaagg tgggaacgtt tcgaatttct
    10381 tcttccccat tttggtttct caccttaatg ggtcgccctt caatatttgc ccatttctta
    10441 tactcttcaa catggtcgcc ctgtgggctg tacatcctgg ggaaaattgt tttgaactgg
    10501 tcgtcaaaat tgtatgtagt ttttaagtag gtttcaacat acttgccgtt cttgggtatg
    10561 cgtgttgttt tatccttact tcctgtaacg ggggcattgt agtattgtcc gtaaaacaaa
    10621 ggcctatctc cgtattgttc acggttaagg tagttaatta gggagaaaac attggatggg
    10681 ttgttttcgt taatcggcgt gttagcattt gccctgagga ttatcatgcc ataggaggag
    10741 tagccaatca atattgctgc aaccgagacg agcaccgtgt ttagcagcac tttcttcttt
    10801 ttatgggagt tgtaaattcc ccatgcaata agggtaaata gtagtatgaa gtagaaaatc
    10861 agcccgctga agtagggtaa tccaaaccca ttaacaaaca ttagctcaaa gagggagcca
    10921 agtttaaatg ttccggggat tataatgtaa agtatcgcca ccaggataac agctgaaact
    10981 agcagggcca caaaaatacc gttgcgcgta ggcttgtatt ttttgtagta gtaaataaat
    11041 gcgagcgccg gaatggtgag caggttgagc aggtgaacgc caatggataa tcccatcatg
    11101 taggcaataa aaatcagcca gcggttggaa tggggctcat cagcaacctc ttcccacttt
    11161 aacattgccc agaaaacaat ggcggtaaag aacgatgaca tggcgtaaac ctcgccctct
    11221 acggccgaga accagaaagt gtcggaaaaa gtataggcaa gtgcacctac tactccgctt
    11281 gccataacag caacaagatt tccggtagaa atattttcgg catctttgat gattagcttg
    11341 cgggcaagat gggtgatggt ccaaaaaaga aacaggattg taaaggcact agctaatgcc
    11401 gacatggcat ttaccaatgc tgccaccttc gtaacatcgg atgcaaacag ggtaaagaaa
    11461 cgggcaatca tcataaaaag aggcgcacca ggaggatgac ccacttcttg tttaaaggct
    11521 gtggcgatga attccccgca gtcccaaaaa cttgcggtag gttcaatagt caaaaggtac
    11581 actactgctg aaatagcaaa tactatccaa ccagtaatgt tgtttacttt tttgaatttt
    11641 cccataggaa agttggtttt gcaaacaatt taacacaatt atggcaacaa atgtagaatt
    11701 tttagaagaa taatggtttg aaaaatgctg aaatctttgg gctaaccatc tgctaccaac
    11761 gaaaaccaca aaaatttact ttaccattag cagctcttta aggttgatta ctaacattta
    11821 ttaaactttg cgcataactt tgcaacatgc cagaagcaga gtgtctggtt aaggtagcat
    11881 taatcaggag ctaactattt ataggcaaaa tgaagagaga ggaacaggat aatgacaagt
    11941 tgaagttcgc ctattcaacc aaccccgatt ttaaggcgag taatgaaatg caaccggagc
    12001 aagaaagtat ttcgcccgaa aagcaaaaat tggtggtatg cattgaacgg aaaaacagag
    12061 gtggaaaatg cgtaacttta gttaagggat tccggggtag caacgatgac ctagacacat
    12121 tagggaaaca actcaaatct agatgtgggg ttggtggatc ggttaaggat ggagaaatta
    12181 tcattcaggg tgagctgcgg caaaaggttg ccgcaattct ccgttcttat gggtataatg
    12241 ttgtagtttc aggttagaaa acaaccctaa ggcattgact atgaaattta tatttcaact
    12301 tttgatattt ttgattctat cctttggtgc tgctgccgat acgcttacag tcatgcatta
    12361 caacctgatg tactacgacc gggaatatga ggagtgtacg gcagcaaaca acaatgttga
    12421 cagcaaggac gaatatctgc gaaccattat tggctatgct aggcccgaca tactaaccgt
    12481 taacgaggta aatgcatccg tttcgagcgt ggagagaatc agaaccaatg ttctaaacat
    12541 aaatggcata actaagtaca aacgtgctaa ttttagcgga agctttctgg caaacatgat
    12601 atacttcaac tccgagaagg tggagcttaa atcgcaggat gttattccta caagtcctcg
    12661 cgaaaccaat gtttacaagt tatacctaaa aacttcctcc cttgtgcagg gcgatactat
    12721 tttcctgtac tgcatgatta cccatctcaa agcaggcagc gatccttcgg acgaggacga
    12781 tcgcaccgct gcatcggctg caattatgag ctacctggct tccagcaacc taaacggaaa
    12841 cgtcttactc atgggagata tgaacctata cagcgcagaa gagggggctt tcgtaaattt
    12901 cactactcct acaggcataa atcagttcag attttatgac ccccttagca tggtggggga
    12961 ttggcataac aactacagct atagaaatat tcacacccaa tccacgcata caacctccga
    13021 cggctgccat tcaactggtg gtatggacga tagattcgac tttattttat cctcatccag
    13081 cttaacaacc ggaaatgatg gtttaaaaat aaccagctat aagactattg cacaggatgg
    13141 taatcgtttt aacgaatcgt taataaaccc tgctaactat agcgttccta ccgatgtgtt
    13201 gagtgcccta tataacaatt ccgatcacct gcccctcaaa atgagccttt actccgattt
    13261 ctccttacat tccgccacaa agtccaacct agtgcctaaa cttgttttta ataatccggc
    13321 aaacaacaag ctgcatgttc atgttcctga aggcatttca attgtaacat tgaatcttta
    13381 cagcatcacg ggtggtaaaa tgctaagcta ccagggtgct gaacctgtgg ttgacatgga
    13441 tatttctgga ttaagcctgg gaatttatat tgccgaggca acactttcgg acggaagtgt
    13501 tagctttttt aaagtgctaa aaattcgata aatatccttt tacgttatta tatatatgtt
    13561 tataaatgag acgattaatt attattttgt caaccatatt tttgattcaa acagcggcta
    13621 aagcgcagta ctaccaaaca ggagttgagc caggttacac aaaatggcgg caaatcgaat
    13681 caggaaaaat tagggtgata tatccttcag aatatgagga taaagcctct agttacttaa
    13741 acttattact aactacagat agccttaccg gtagggacta taacctgaag tcaaaaagga
    13801 ttgatgtggt tcttcatccc aactccatgc tatcgaacgg ttttgtggca tgggctccac
    13861 gtaggatgga gcttattact tttccatcta ccgatggtta cgctcaaccc tggagtagtc
    13921 aacttgcttt acacgagatg cgccacgtta agcaaatgta tgcgctcaac tccagcacat
    13981 cccgactggc atactatctt tttggggagc aggtagtcgg cttagcggct ggactagttc
    14041 cccaatggtt tcttgagggt gatgccgtag taacggaaac tgcccactcc ttctctggaa
    14101 ggggtcgttc ggcaacattc tggcagcatt accgtgctca ctttttggcc gatagaaaga
    14161 cctactcata taacaagtgg ctgttgggat cctataaaaa ttacattcct aatagctata
    14221 gcttaggtta tcagctggtt agttttggta attttaagta tgggattaat acttggccaa
    14281 aggttgtaaa ccatgtggcc aagtatcctt ttacagctat tccattttat tttggtgtga
    14341 agcaaaatac aggacttccg gctaggaagc tggccgatag cacctttgca tatctgattg
    14401 gtagatggaa ggccgatacg gttgaccggt atctttcagc cgttaaccat cctgttagaa
    14461 tgcctaagtc atataccagc tacctctacc cctacaagct taatgactcc accatgctgg
    14521 cctaccgaac ctcactaaac gacataccag cgtttgttga aataaacctg aacagccaaa
    14581 aggttaagga gctatgctat ccagggtatc tccttggtcg cccaacctat tcggacagcc
    14641 ttattgtttg ggctgaatat cagccacata cccgatggga gtacgaagac tttggcagga
    14701 tttatggatt aaggataaag gacgtccaaa aaattgaact aagtgggaaa ggcaaatact
    14761 tttcgccagc cattaaccca gacgactgtt cagttctatg catttcctac agtcctgatg
    14821 gtaactttaa gctggtttcc attggaaaaa accgtgagga gcgagaattg ctgagttttt
    14881 caagtggata cgaagttcag gagctttcgt tcgatagaaa tacacgaatg ctatatgcac
    14941 ttgtagttac ccagcagggc aagg
//
LOCUS       DAOPHX010000058        14910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_4609_length_14910_cov_6.492225, whole genome shotgun sequence.
ACCESSION   DAOPHX010000058 DAOPHX010000000
VERSION     DAOPHX010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 14910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14910
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4609_length_14910_cov_6.492225"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PK502_05795"
     CDS             complement(<1..609)
                     /locus_tag="PK502_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPH00362.1"
                     /translation="MQKVLVTGGAGYIGSVLVRQLLEHNYRVVVLDSLMYGGESLLDL
                     FNNNNFDFVKGDIRREEDIAMAMKGVDFVVHLAAIVGDPACAKQPELAKSTNLDGSKL
                     LYSIANKMGVKRFIFSSTCSNYGRMANPDIMVDEMSELAPVSLYAETKVGVEKFLLSQ
                     PKSNTCKPTVLRFSTVYGLSLRPRFDLTVNEFTKELALGRELV"
     gene            complement(680..1561)
                     /gene="folD"
                     /locus_tag="PK502_05800"
     CDS             complement(680..1561)
                     /gene="folD"
                     /locus_tag="PK502_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HPH00363.1"
                     /translation="MELLDGKKISEEIRAEIASEVEKIKSQGGKIPYLVAMLVGHDGA
                     SETYVHNKELACAQVGFRSQVLRFEETISESGLLAEIQKLNANPEVDGFIVQMPLPKH
                     IDEQKIIEAIDPKKDVDGFHPVNVGRMVIGLPSYISATPDGIVELLRRYKVETAGKHC
                     VVLGRSNIVGRPVANLLLHKGYPGDCTVTICHSRTPNLKELTLQADILIVAMGKPEFV
                     TADMVKNGTVVVDVGITRIPSEKTKSGWKLVGDVKFDEVAPKCSFITPVPGGVGPLTI
                     ASLLKNTLKAARKEVYG"
     gene            complement(1697..3031)
                     /gene="ffh"
                     /locus_tag="PK502_05805"
     CDS             complement(1697..3031)
                     /gene="ffh"
                     /locus_tag="PK502_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPH00364.1"
                     /translation="MFENLTEKLERSFKILKGEGKITEINVAETLKEVRKALLDADVN
                     YKVAKSFTDEVKRKALGMNVLNAIRPGQLMVKVVHDELAELMGGKAANINLNGNPAII
                     LIAGLQGSGKTTFSAKLASYLKTKKGKSPLLVAGDVYRPAAIEQLKVLGDQVGVPIFT
                     VPGSSNPVDIAKLSIKQAKTLGRDTIIIDTAGRLAVDEEMMNEVAAIKKVVSPQEILF
                     VVDSMTGQDAVNTAKEFNDRLNFDGVVLTKLDGDTRGGAALSIRAVVDKPIKFISTGE
                     KLDALDVFHPERMADRILGMGDIVSLVEKAQEQFDADKARELNKKILKNRFNFNDFLE
                     QIGQIKKMGNLKDLAGMIPGVGKAIKDVDLNDDAFKSTEAIIKSMTPAEREEPSLING
                     SRRKRIADGSGTSIVEVNRLIKQFEDMRKMMKMASGGGAKNLARMMSQQPRR"
     gene            complement(3150..4946)
                     /gene="argS"
                     /locus_tag="PK502_05810"
     CDS             complement(3150..4946)
                     /gene="argS"
                     /locus_tag="PK502_05810"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPH00365.1"
                     /translation="MSTEQYIQDIVAETVLKLYGVKPEVNQLQIQKTRKEFEGDYTLV
                     AFPLLKLTRKSPEETCRQIGEMLLQSYPEVSAINVIKGFLNITLSKTYWSGVINEIAS
                     TEDYGFAPTPTENANPTIVEFSSPNTNKPLHLGHIRNNLLGFSISRILEKSGKRVIKV
                     NLVNDRGIHICKSMVAWLKYGNGETPQTSGKKGDHLVGDYYVRFEKEYKLQVEQLTSS
                     GMDKDEALTKAPIIIEAQEMLRKWEAKDAETIRLWKMMNGWVYEGFEKTYQNLGITFD
                     KIYYESQTYLVGKKVVNEGLDRGIFFRKSDGSVWVDLTADGLDQKLLLRSDGTSVYIT
                     QDIGTAIERYNEFHFNEHIYVVGNEQDYHFQVLKLVMKKLGYTWYEALYHLSYGMVLL
                     PEGKMKSREGTVVDADDLISEMLQTAGDVSRELGKLDQMSDEEASETIRMVGLGALKY
                     FILKVDPKKNMTFNPGESIDFNGNTGPFIQYTHARIRSVLRKAGNVNDLLNQKVTPES
                     ISFQKELELIQLLHLFPDTIKIAAQTHNPAEIANYTYELAKQYNQFYQDLPILREPNV
                     EVKHFRLLLSQAVARVIKQAMWLLGIDVPEVM"
     gene            complement(5085..8366)
                     /gene="secA"
                     /locus_tag="PK502_05815"
     CDS             complement(5085..8366)
                     /gene="secA"
                     /locus_tag="PK502_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPH00366.1"
                     /translation="MAGGSFLSKIFGTKADRDMRELMPMLNTIRETYKKYPSLSNDEL
                     RSESAKLKAKVHDYIAADEAEVERLKQMMESDEIGYDEKEKIYKDVEKLQQTIDDKIE
                     EILNIVLPDAFSIVKETARRFKENETIEVTATQFDKDLAAQKDFVTIQDDKAIYKNRW
                     MAGGNEIVWDMVHYDVQLIGGVVLHQGKIAEMATGEGKTLVATLPVFLNALAGKGVHV
                     VTVNDYLARRDSEWMGPIYEFHGFAVDCIDKHQPNSEARRKAYNADITFGTNNEFGFD
                     YLRDNMAIAPDDLVQRKHHFAIVDEVDSVLIDDARTPLIISGPVPKGDDQMFEEYKPK
                     VEKLVNAQRALITQLLADAKKLIAEGNTDEGGKLLLRAFKGMPKYKPLIKFLSEQGNK
                     ALLLKTENYYMQDNNRNMHLITDDLFFVIDEKLNSVELTDKGNDLITSSSEDSKFFIL
                     PDIGAEIAEIEKMPISHNEKLAQKDELLRDFSVKSERVHTVHQLLKAYTMFEKDVEYV
                     VMDNKVKIVDEQTGRILEGRRYSDGLHQAIEAKERVKVEAATQTFATITLQNYFRMYH
                     KLAGMTGTAETEAGEFWTIYKLDVVVIPTNQPVIRKDMEDLVYKTKREKYNAVIDEIV
                     KLTGENRPVLVGTTSVEVSELLSRMLKLKGIKHNVLNAKQHQREAEIVAQAGLPKTVT
                     IATNMAGRGTDIKLTPETRAAGGLAIIGTERHESRRVDRQLRGRSGRQGDPGTSQFYV
                     SLEDDLMRLFGSDRIARIMDRLGLKEGEVIQHPMISRSIERAQKKVEENNFGIRKRLL
                     EYDDVMNAQREVIYTRRRHALYGERLDIDISNMFWDVCEAMVSSFKGSESYEDFSLET
                     IKNFSLEAPVTEEEYENLKPDEIAERLYHQALESYDRKAASIAKQAYPVIKDVYEKQS
                     AIYENIVVPISDGQKTYQIVTNLKKAYESGGKELIRSFSKSIILYTIDEYWKEHLREM
                     DDLKQSVQNAAYEQKDPLLIYKLEAYELFRTMLENLNRDIVSILAKAFIPLRSANEVQ
                     EARQRSHTDMSRYQTSRTDNLQAGAPTQQPNREPVKVGIKVGRNDPCPCGSGKKYKNC
                     HGRDE"
     gene            complement(8782..10512)
                     /locus_tag="PK502_05820"
     CDS             complement(8782..10512)
                     /locus_tag="PK502_05820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPH00367.1"
                     /translation="MENFIVSARKYRPSTFKSVVGQTSITTTLKNAIQRKQLAQAYLF
                     CGPRGVGKTTCARIFAKTINCLNLTDENEACNECESCKSFSTNRSFNIHELDAASNNS
                     VEGIRKLIEQVRIPPQIGKYSVYIIDEVHMLSASAFNAFLKTLEEPPAHAIFIMATTE
                     KHKILPTILSRCQIFDFNRIRVEDTVKYLEYIAKTEGIDYEVEALNIIAQKADGAMRD
                     ALSIFDQVVSFSENHITYEKVIENLNVLDYQYYFRLTDNFLSGNMTDSLKIFDELLSK
                     GFDAQQFITGLSQHLRNLLVCKDSDTIQLLEVGAAIAEKYTKMASTCPIDFLVNALQL
                     SNQYDVSFRQSPNQRLHVELLLIRLCGLTQEKKKSESPANIEEGDVVEQKPQKQTKPA
                     QKVKHETYEPAAVNEPSTPIIPKTTSLKGALKKNTVESYTASDIDQPEIPSVAVNLPN
                     DSEYKAITQDMLGKAWSSFAESIKEEKPGQYSLLQLYGPELLSDGKAYIRFETQIQVD
                     MFREIKTDLLTFLKQSLKVNPPEILEEIAETDEKNKPRLFTADDKLKFLLQHNPALRK
                     MKQKLNLDLE"
     gene            complement(10639..11799)
                     /locus_tag="PK502_05825"
     CDS             complement(10639..11799)
                     /locus_tag="PK502_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPH00368.1"
                     /translation="MKPFQMFVFITITLGVYLLVNLFIYYQTRVVFSIPRWGILLKII
                     FLALVLAYPVGRGLEAIFDGNTFTPLVRIGSMWLGAMLYLFMAFLVVWIVVVSTHTFG
                     SANPIHARQVGVALVYALVSILLIAGYTNAITPIVRKIEVRVDKPLRGGKLRVVAVSD
                     IHLGTVIGKRDLAKLVKMANAQNPDVVLLVGDIFDEDVTPVINGGMGTLLEQFKSKYG
                     VFAVTGNHEFYGGFEQKIKYLQQHGVKLLRDSTVLVDSSFYIIGRDDRQSSYMLKRER
                     KSVEELISEVDLRKLTILLDHQPYNLDDAARCGVDLQLSGHTHNGQLWPFNYITKAMY
                     EVSHGYYQKGGTHYYVSSGFGTWGPRLKIGNRSEVMVVEITGNPQNSTPEAS"
     gene            complement(12097..12507)
                     /gene="rnk"
                     /locus_tag="PK502_05830"
     CDS             complement(12097..12507)
                     /gene="rnk"
                     /locus_tag="PK502_05830"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HPH00369.1"
                     /translation="MSRITLNRLDYERIKRHLEVARQLKSITNTEADKLLGELESAQV
                     VNPESIPSDVVTMNSIVKISFLNTGKEIQFRIVYPHEANLKENKISIFSPIATALIGY
                     RVGDEIEWLVPAGPTKIRIEEIVYQPEASGNFTL"
     assembly_gap    13249..13348
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13435..13508)
                     /locus_tag="PK502_05835"
     tRNA            complement(13435..13508)
                     /locus_tag="PK502_05835"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13472..13474),aa:Arg,seq:tct)
     gene            complement(13993..14451)
                     /locus_tag="PK502_05840"
     CDS             complement(13993..14451)
                     /locus_tag="PK502_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00370.1"
                     /translation="MTTKNKIDKMFGPVGTLAGIFLFVAGSIITYFSIAGLVVVLIGA
                     FVGFTSTITSIDFGKKRLRFSIYIFGLFPIGKWILIQSDMKIGIKKSTKLWRTYSQSN
                     RTLDIADNDYRLILYDSNGKKIMPIQKSGNLNSAKFNLDKLSKQLEIGVI"
     gene            complement(14635..>14910)
                     /locus_tag="PK502_05845"
     CDS             complement(14635..>14910)
                     /locus_tag="PK502_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00371.1"
                     /translation="LKAFPIRELVQIFRKNGHPITLKTELKITGVYNSGDTSGIMCTV
                     EIADGYKMACALTHLIFLRSDKLYSDIMDYQRERVKRIKQLNLTGLN"
BASE COUNT         3571 a         3412 c         3311 g         4516 t
ORIGIN      
        1 aaccagctcc cttcccaggg ccaactcctt tgtaaactca tttactgtca ggtcaaatcg
       61 tggccgtaag gataacccgt aaacagtgga gaatcgaaga acagttggtt tacacgtgtt
      121 ggatttgggt tgcgatagta aaaacttttc tactccaact ttggtttcgg cgtatagtga
      181 aactggtgca agttccgaca tctcgtcaac cataatatcg gggttggcca ttctaccgta
      241 attactgcag gttgaggaaa aaataaaacg ttttaccccc attttattgg caattgagta
      301 gagtagcttg ctcccatcga ggttagtgct tttggctaat tcgggttgtt tggcacaggc
      361 gggatcgcca actatagcag caaggtgcac aacaaaatct acccctttca tagccatggc
      421 aatatcttct tcacgtcgaa tatcgccttt tacaaaatca aaattattgt tgttgaataa
      481 atcaagcaga gattctcctc cgtacattaa gctatccaaa acaaccaccc tataattatg
      541 ctccagaagt tgacgcacca aaactgaacc gatgtatccc gctccacctg ttactagtac
      601 tttttgcatt gactaatttt gttaatgatg taataaagtt ttgatttttc tttatgcaca
      661 ggagaagtta atgcctatgc tatccgtaaa cctccttcct ggctgccttg agggtatttt
      721 tgagcaacga ggcaatggtg aggggaccaa caccaccggg taccggggta ataaagctgc
      781 atttaggcgc cacctcatcg aatttaacat cgcccaccag cttccatccc gatttggttt
      841 tttcgcttgg aattctggtt atccccacat caaccactac tgtaccattt ttaaccatgt
      901 cggctgtaac aaattcaggc tttcccatgg ctacaattag tatatcggcc tgcagggtaa
      961 gttcttttaa gttgggtgtt cggctgtggc agatagtaac agtgcaatcg ccagggtagc
     1021 ccttgtgaag cagcaggttg gctacagggc gacctacaat gttgctccta cccagcacca
     1081 cacagtgttt tccggcagtt tcaaccttat accttcggag tagctccaca attccatcgg
     1141 gtgttgccga aatgtaggaa ggaagcccaa taaccatccg acccacgttt accgggtgaa
     1201 agccatcaac atcctttttg gggtcaatgg cctcaataat tttttgctca tcaatatgct
     1261 tgggaagtgg catctgcact atgaagccat ccacctcggg gttggcattc agcttttgta
     1321 tttcagccag tagcccgctc tcactaattg tctcctcaaa gcgtagcacc tgcgacctga
     1381 atcctacctg ggcgcaggcc agctccttgt tgtgaacgta ggtctcgctt gccccatcat
     1441 ggccaaccag catggctacc aggtagggga tttttccccc ttggcttttt atcttttcaa
     1501 cctccgaggc gatttcggct cgaatttctt cagatatttt tttcccatct agtagttcca
     1561 ttattcaaga ttcaagattc aagattcaag gtttaaggtt taaggtttaa ggcatccatc
     1621 ttcctcccct taacttttca gggtttacga tttcgctttt atccttgtat tatttaatat
     1681 ctcactctac ttttctctac cttcttggct gttgtgacat catccgtgca agatttttcg
     1741 caccacctcc ggatgccatc ttcatcattt tacgcatatc ctcaaactgc ttaatcaacc
     1801 ggtttacctc cacaatggag gttccgctgc catcggcaat tcgcttgcgg cggctaccat
     1861 taatcaacga cggttcttca cgttcagccg gggtcatgga tttaataatg gcctcggtgc
     1921 ttttaaaggc atcatcgttt agatcaacat cttttatggc cttacccact ccgggtatca
     1981 ttccggcaag gtccttcagg tttcccattt tctttatctg gcctatctgt tcaagaaaat
     2041 cgttaaagtt gaacctgttt ttaagtatct tcttgttaag ttcccgggct ttatccgcat
     2101 caaactgttc ctgcgctttt tcaaccagcg aaacaatatc gcccattccc agtatgcggt
     2161 cggccatacg ttcgggatga aaaacatcga gggcatcgag cttctcgcct gtgctgataa
     2221 acttaatggg cttgtcaaca acggccctta tggatagagc tgcaccaccg cgggtatcgc
     2281 catcgagctt ggtgagcacc acgccgtcaa agttaagcct gtcgttgaat tcttttgctg
     2341 tgttaaccgc atcctgaccg gtcatggagt caactacgaa tagaatttcc tgaggcgaaa
     2401 cgaccttttt gatggcggcc acctcgttca tcatctcctc atcaactgcc agacgaccgg
     2461 cagtatcaat tataatcgta tctcttccca gcgttttggc ttgtttaata gagagtttag
     2521 ctatgtcaac gggattactg ctacctggaa cggtgaaaat tggaacgcca acctgatcgc
     2581 caagcacctt aagctgctca atagctgccg gtctgtaaac gtcaccggca accagcaacg
     2641 gcgattttcc ctttttggtt ttgaggtagc ttgccagctt ggcagagaaa gttgttttac
     2701 cggagccttg aaggccggca atcagaataa tcgctgggtt tccgttaagg ttgatattgg
     2761 ctgccttacc acccattagc tcagctagct cgtcatgaac tacttttacc attagctgtc
     2821 caggcctgat ggcattaagc acattcattc ccaaggcttt tcgctttacc tcatcggtaa
     2881 aactcttcgc aactttatag ttgacatcgg catcgagaag agctttacga acttccttaa
     2941 gggtttcggc tacgtttatt tcggttattt tcccctctcc cttgaggatt ttaaaggatc
     3001 gttcgagctt ttcggttaaa ttttcaaaca taaattgttt tactatttat tattctctgc
     3061 aaagttatca aaaatgatgg ctttttcaac ataaagccat tttggaagtg cagctaaaag
     3121 gctatgttta gataaagctt ctgttatatc tacataactt ccggcacatc aattcccaga
     3181 agccacattg cttgtttaat aacacgggct actgcttgcg aaagcagaag cctaaagtgt
     3241 tttacctcga cgttgggttc tcgcagtatg ggcaggtctt ggtaaaactg gttgtactgc
     3301 tttgccagct cgtaggtata gttggcaatt tctgctgggt tatgcgtttg agctgctatt
     3361 ttaattgtat cggggaaaag atgtaggagt tgaatgagtt ccagttcctt ttgaaatgaa
     3421 atgctttcgg gtgttacttt ttggttcagt aaatcattca cattgcccgc cttacgaaga
     3481 acggagcgta tgcgtgcatg ggtgtactgg atgaatgggc cggtgttgcc gttgaagtca
     3541 atagactctc cagggttgaa ggtcatattt tttttggggt caaccttcag gatgaagtac
     3601 ttaagtgctc ccaggccaac cattctgatt gtttctgaag cctcctcatc gctcatctgg
     3661 tctagcttgc caagttctcg cgatacatca ccggcagttt gaagcatttc ggatatgagg
     3721 tcgtcggcat caaccacggt tccctcgcgc gatttcatct ttccttcggg cagcagcacc
     3781 attccgtagg agaggtggta tagagcctcg taccatgtgt agccgagttt tttcataacc
     3841 agcttaagca cctgaaaatg gtagtcctgc tcattgccta ccacgtaaat atgctcgtta
     3901 aaatggaact cgttgtagcg ctctatcgct gtgccaatat cctgggtgat gtaaactgaa
     3961 gttccgtcgg agcgaagcag tagcttttgg tcgaggccgt cggcggtgag atcgacccaa
     4021 accgaaccat cgctcttcct aaagaaaatt ccccggtcca acccctcgtt cactaccttt
     4081 ttcccaacca ggtaggtttg cgattcgtag tatattttgt cgaaggttat tcctagattt
     4141 tggtaggttt tttcgaaacc ttcataaacc caaccattca tcattttcca aagcctgatg
     4201 gtttcggcat ctttagcttc ccattttctg agcatttcct gagcttctat gattataggt
     4261 gccttggtta atgcctcgtc tttgtccatg ccgcttgacg tgagctgctc tacttgcagc
     4321 ttatactcct tttcaaacct gacgtagtag tcacctacca ggtgatcgcc ctttttacct
     4381 gacgtttggg gtgtttcgcc atttccgtac ttaagccaag ctaccatcga tttgcaaatg
     4441 tggattcccc tatcgttcac caggtttacc ttaataaccc ttttcccgct tttctccagt
     4501 atcctcgaaa tggaaaaccc cagaaggttg ttgcggatat gtcccaggtg aaggggctta
     4561 ttggtgttgg gcgacgaaaa ttctacaatg gtagggttgg cattttctgt tggggtaggg
     4621 gcaaatccat agtcttcggt tgaggcaatc tcgttgatta cgcctgacca gtacgtttta
     4681 gatagggtga tattcagaaa cccctttatt acgttaattg ctgaaacctc tggatacgat
     4741 tgaagtagca tttccccaat ctggcggcag gtttcttcgg gcgattttcg cgtaagcttc
     4801 aacagtggaa aggcgaccag agtgtagtcg ccttcaaact ccttacgtgt tttttgaatt
     4861 tggagctggt ttacttccgg ttttactccg taaagtttta gaacggtttc tgctacaata
     4921 tcctgaatgt actgctcggt tgacattatt tggttttgtt tgaaatccat gcttcctgat
     4981 gctgaagtaa taaaatggct gtgtttgcaa aagccactgc tccacagcca gaattatacc
     5041 cgatgttgat aggtagcttt agcccgacag gcgagcgtaa aattctactc gtctcttccg
     5101 tggcagtttt tatatttttt cccgctaccg catgggcagg ggtcgtttct accaaccttg
     5161 atgcctacct taacaggttc cctgttgggc tgttgtgttg gtgcacctgc ctgaaggttg
     5221 tccgttcgtg aggtttgata cctactcatg tcggtgtggc tacgttgtct tgcctcctga
     5281 acctcgtttg cactccgtaa cgggataaag gccttggcaa gtatggacac aatgtcgcgg
     5341 ttcaggtttt cgagcatggt tcggaacagc tcgtaggctt caagtttata tatcaacagg
     5401 ggatccttct gctcgtaggc ggcattctgc acgctttgct ttaggtcgtc catttcacga
     5461 agatgttcct tccagtactc atcaatggtg tagaggatta tggattttga aaacgaccta
     5521 atgagctcct tacctcccga ttcgtaggcc tttttcaggt tggtgacaat ctggtaggtt
     5581 ttttggccat cagaaattgg caccacgatg ttctcgtata ttgccgattg tttttcgtaa
     5641 acatccttaa tgacgggata ggcctgcttt gcaatgctgg cagcttttct atcgtacgat
     5701 tcaagcgctt gatggtatag ccgttcggca atttcgtctg gtttaaggtt ttcgtattct
     5761 tcttctgtaa caggagcttc aagagagaaa ttcttgatgg tttccaggga gaagtcttcg
     5821 tagctttcgc ttcccttgaa tgaggaaacc attgcctcgc aaacatccca gaacatgttc
     5881 gaaatatcaa tatccagcct ttcgccgtaa agtgcatggc ggcgacgggt atagatcacc
     5941 tcgcgctggg cattcataac gtcgtcgtat tccagcaacc gcttacgaat accaaagttg
     6001 ttctcctcaa cttttttctg agcccgttca attgacctgg aaatcattgg gtgctggata
     6061 acctcgcctt cctttagtcc cagcctatcc attattctgg caattctgtc agatccaaaa
     6121 agccgcatca ggtcatcttc cagggatacg tagaactgtg aggttccggg gtcgccctga
     6181 cggccagaac gcccacgtag ctgacggtcc acgcgtctgg actcatgccg ctcggtacca
     6241 atgattgcta gaccacctgc agcacgggtt tcgggggtta gcttgatgtc ggtaccacga
     6301 ccagccatat tggtggctat ggtaaccgtt ttcggtagcc ctgcttgtgc cacaatttct
     6361 gcttcacgct ggtgctgctt tgcattcagc acgttgtgct taattccttt taacttcagc
     6421 attcggctga gcagctccga tacctcaacc gaggttgttc ccacaagtac cggcctgttc
     6481 tcaccggtca gctttacaat ttcgtcaata accgcattat acttttcgcg cttagtcttg
     6541 tataccaaat cctccatatc cttacggata acgggttggt tggtaggaat aaccacaaca
     6601 tcgagcttgt agattgtcca gaactcgccc gcttcggttt cagcggttcc ggtcatacct
     6661 gccagcttat ggtacatcct gaagtagttc tgcagggtaa tggtggcaaa ggtttgagtg
     6721 gcagcttcaa ccttaaccct ttcctttgct tcaattgcct ggtgcaatcc atcggagtac
     6781 cgacgccctt cgagaatacg acctgtttgc tcgtcaacaa tttttacctt gttgtccatc
     6841 accacgtact ccacatcctt ttcgaacatg gtgtaggcct tgagcagctg gtgaacggtg
     6901 tgcacgcgtt cggactttac ggagaaatcg cgtaggagct catccttttg tgctaacttc
     6961 tcgttatggc tgatgggcat tttttcaatt tcggcaattt cagcaccaat atcagggagg
     7021 atgaaaaact tggaatcctc cgatgagctg gttattaggt cgttgccctt atcagtaagc
     7081 tcaacggagt taagcttttc gtcaataaca aagaacaggt cgtcggtaat caggtgcatg
     7141 ttccggttgt tatcctgcat gtagtagttt tcggttttaa ggagcagcgc cttgtttccc
     7201 tgttcgctaa ggaatttgat gaggggttta tacttgggca tacccttaaa tgccctgagc
     7261 agtaattttc caccctcatc ggtgttgccc tcggcaatta actttttagc atcggctaaa
     7321 agctgggtaa tcagggctcg ctgagcattt accagctttt ctaccttggg cttgtactcc
     7381 tcaaacatct ggtcgtcgcc tttgggcact ggtcccgaaa tgatgagggg ggttcgtgcg
     7441 tcgtcaatca gcaccgagtc aacctcatcc acaatggcaa agtggtgctt gcgctgcact
     7501 aaatcatcgg gggcaatggc catgttgtcg cgcaggtagt cgaagccaaa ctcgttgttg
     7561 gttccaaagg taatgtcggc gttgtatgct ttccggcgtg cttcagagtt aggttgatgc
     7621 ttatcgatgc agtcaaccgc aaagccgtgg aactcgtaga ttggccccat ccactccgag
     7681 tcgcggcgag ccaggtaatc gttaacggtt acaacgtgaa ctcctttgcc ggccaaggca
     7741 ttcaggaata ctggaagggt tgccaccagg gttttccctt caccggttgc catttcggca
     7801 attttgccct gatgcagaac tactccgcca atcagctgta catcgtagtg aaccatgtcc
     7861 caaactatct cattaccacc ggccatccat cgattcttgt aaatagcctt atcatcctga
     7921 atggtaacaa agtccttttg ggcggctaag tccttatcga attgggtggc tgtaacctcg
     7981 atggtttcat tttccttaaa cctgcgcgcg gtttccttca caatggagaa ggcatccgga
     8041 agcactatat ttaggatctc ctcaatttta tcgtcaatgg tttgctgaag tttctcaaca
     8101 tccttgtaaa tcttttcctt ttcgtcatag ccaatctcgt ctgattccat catttgcttc
     8161 agacgttcaa cttcggcttc atcagcagca atgtaatcgt gcactttagc ctttagtttg
     8221 gccgattcgc ttctgagctc atcattcgat aacgaggggt atttcttgta ggtttcccgt
     8281 attgtattga gcattggcat aagctcccgc atgtcacggt cagcctttgt cccaaatatt
     8341 tttgataaaa aacttccgcc tgccatttta atttaattag tttgaagtta acagtattta
     8401 aaaccagcaa atttaaccgt tttttccgaa taattctgaa tgccggggtt tgattgcgtt
     8461 aaaaaatact agattggaga gccaagtgtt tttactataa tttttttgaa atgggaaagg
     8521 gtttggccaa caatcaaaaa tagtcaacat agcctagcat tctgcttagc gtcgagctca
     8581 aaattcgaac agcttggttg agaataaaaa attttttgta cagaagtgtc aaggcgttcc
     8641 ggtttattta ttactgtagc ccaagcatta aagttgattg ttgcgcacct ggtttgatga
     8701 tggttgctag cgttaatcgt ttgtgaactg caatagcctt ttattttcct ctaaatattg
     8761 cgcggtaatg tgctgtttgg gctattcgag gtcaaggttt aatttttgct tcattttccg
     8821 gagcgcaggg ttatgctgta gcaaaaattt aagcttatcg tcggcggtaa aaagccgtgg
     8881 tttattcttt tcgtctgttt cagcaatttc ctctaaaatc tcgggtgggt ttacttttag
     8941 ggattgtttc agaaaggtga gcaggtcggt tttaatctcc ctgaacatgt caacctgaat
     9001 ttgggtttca aacctgatgt aagctttgcc atcgctgaga agttctggac cgtaaagttg
     9061 aagcaggcta tactgtcccg gtttctcctc cttaatcgac tcggcaaagc tactccatgc
     9121 cttgccgagc atatcctggg ttatagcttt atactcggaa tcgttgggca ggttaacagc
     9181 aacactcgga atttccggtt ggtcaatatc gctagcggtg tagctttcta ctgtattctt
     9241 tttaagcgca cctttaagcg aagtggtttt gggaattatt ggggttgaag gttcatttac
     9301 tgcagctggc tcgtaggtct catgtttaac tttctgtgct ggttttgtct gtttttgtgg
     9361 tttttgttca accacatcgc cttcctctat gttggctggg ctttcagatt tttttttttc
     9421 ttgagtaagc ccgcagagcc taatcagcag caactcaacg tgcagccttt ggttgggact
     9481 ttgtcggaaa gacacatcat actgatttga cagctgcagt gcattaacga gaaaatcgat
     9541 agggcaagtg gaggccattt tggtgtattt ctcagctatg gcagcaccca cctccagtag
     9601 ctgaatggta tcggaatcct tgcacaccaa caaattcctt aaatgctggc ttaatccggt
     9661 gataaattgc tgggcatcga atcccttcga tagtagctcg tcgaatatct tcagcgaatc
     9721 agtcatgttc ccactcagaa agttatcggt caacctgaag tagtactgat agtccagcac
     9781 gttgagattt tctattactt tttcgtaggt aatatggttc tcgctgaagc taactacctg
     9841 atcgaagatg gagagggcat cgcgcatggc gccatcagct ttttgggcaa tgatgttcaa
     9901 tgcttcaacc tcataatcaa tgccttcagt tttggcaatg tactcgaggt acttaacggt
     9961 gtcctcaacc cttatccggt tgaagtcgaa aatttggcaa cgcgacagga tggtgggaag
    10021 aattttatgc ttctcggtgg tggccatgat gaaaatggca tgggctggcg gttcctcgag
    10081 tgttttcaga aaggcgttga aagcgcttgc ggacagcatg tgcacctcat caatgatgta
    10141 aacgctgtac ttcccaattt ggggaggtat gcgaacctgc tcaataagct tgcggatacc
    10201 ctctacggag ttattggagg cagcgtcgag ttcgtggatg ttaaatgacc tgttggtgct
    10261 aaacgacttg caactttcgc actcgttgca tgcctcattt tcatcggtaa gattcaggca
    10321 gttgatggtt tttgcaaaaa tgcgggcaca ggtagtttta ccaactcccc gaggaccgca
    10381 gaaaaggtaa gcttgggcca gctgtttgcg ctgaatggcg ttttttagag ttgtggttat
    10441 tgacgtctga ccaacgacac ttttaaatgt tgaagggcgg tacttacgag ccgatacaat
    10501 aaagttctcc atgcgttact tccagattga tggtgcggtt tgccatcaca aagttaatac
    10561 atacaccgaa actttcaagc caaaaattag ctgttggcca atttggctga taaccagcaa
    10621 agtatgcgct aagtcgatct agcttgcttc cggagtagaa ttctgcgggt taccggtgat
    10681 ttcgacaacc attacctcgg agcgatttcc aattttaagc cttggacccc atgtaccgaa
    10741 acccgaagaa acatagtagt gggtgcctcc cttctggtag tatccatggc taacctcata
    10801 cattgccttg gtgatgtagt tgaaaggcca cagctgtccg ttatgggtgt gccccgatag
    10861 ctgcaggtca acaccacacc gggctgcatc atccaggttg tagggctggt ggtctaaaag
    10921 tatggttagt ttccgaaggt caacttccga aatcagctct tcaaccgatt ttctctccct
    10981 tttcagcatg tagctgcttt gccggtcatc tcgaccaatg atgtagaaag agctgtcaac
    11041 cagcacggtt gaatcgcgta gcagctttac gccatgctgc tgaaggtatt ttatcttttg
    11101 ctcgaatccg ccataaaact cgtggttccc ggtaacggcg aaaaccccgt acttagactt
    11161 aaattgctcc agcaaagttc ccatcccacc atttattaca ggtgttacat cctcatcgaa
    11221 tatgtcgccc accagcagca ccacgtcggg attttgagcg ttagccatct tcacaagctt
    11281 tgccagatca cgcttgccaa ttactgtccc cagatgaata tctgaaacgg ctactacgcg
    11341 gagtttaccg ccacgcaatg gcttgtcaac cctaacttca atctttctaa ctatgggggt
    11401 tatggcgttg gtgtaaccag ctattagcag aattgaaacc aaagcgtaaa ccaaggccac
    11461 acctacctgc cgggcgtgaa tcggattagc cgatccaaag gtatgggttg aaaccactac
    11521 tatccaaaca acaaggaaag ccatgaacag gtatagcatg gcacctagcc acatggagcc
    11581 tatacgaaca agcggggtaa atgtatttcc atcaaaaatg gcttctagcc ctctccctac
    11641 ggggtaggct agcaccaggg caagaaagat aatttttagc aaaatacccc atcggggtat
    11701 ggagaaaacc acccgggttt gatagtaaat gaaaaggttt accagcaggt aaactccaag
    11761 ggtgatggta atgaatacga acatctgaaa tggcttcatc cgtttaaatt atttactgca
    11821 agtgtgagct aatctactgt tctgcccgcg aaaatacgtt gcataaacat ataattgcat
    11881 tcaatgttca ccgtattcat aaaacatgct gaatcatagc tatttgtaat caagtagatt
    11941 tatcggcaac ctaaggcaca gtaggcataa acaggctcca gggttccacc cggagcgaaa
    12001 ttttacgggc agctgaatca tacatagcaa gccctgcctg atactggaat gaatttgctt
    12061 acaggcaggg agtacaaagt ttacattcaa tttcgcttaa agggtaaagt tgcccgaggc
    12121 ttcaggttga tagactatct cctcaattcg tattttggta ggaccggcgg gcaccagcca
    12181 ttcaatctca tcgcctaccc tatagccaat tagcgctgtg gcaatgggtg aaaatataga
    12241 gattttgttc tcctttaagt tagcttcgtg ggggtacaca attctaaatt gaatctcctt
    12301 gccggtgttc aaaaagctga ttttgacaat tgagttcatg gtaacaacat ctgagggaat
    12361 gctttctggg ttaactactt gggccgactc cagctcccca agcagcttat cggcttcggt
    12421 gttggtgatt gatttgagtt gcctggcaac ttcaagatgc ctcttaattc gttcatagtc
    12481 aagtcggttc aatgttatcc tgctcatggt ttcatgattt tagtataatt tgaaacgtga
    12541 taaagatagg caatgtttac gtgtaaatga aatcttgagg ggaggtgttt taggtggcac
    12601 gctagcttca gctggatgat ttttctgtac cattctgagc tactttaccc ataacctaca
    12661 tagggttagc aggttataat ctggctttgc aaaaacctgt tgttaaactg gcgattcctc
    12721 cctgtgggct ggtgggcgag gaaggttttg atcaggtgat ccgggcggga ttagctcgcc
    12781 tgcggctcgt gatcccgccc tgcctgaatt gaggagggga gtggcttccc catcgtgatc
    12841 cgggcgggat tagctcgcct gcggctcgtg atcccgccct gccgtaacgc aaaaagtaaa
    12901 ggcagttcaa gttcagaatt gccttggata cgcgtagtgt ttctcaggtg atccgggcgg
    12961 gattagctcg cctgcggctc gtgatcccgc cctgcctgaa ttgaggaggg gagtggcttc
    13021 cccatcgtga tccgggcggg attagctcgc ctgcggctcg tgatcccgcc ctgccgtaac
    13081 gcaaaaagta aaggcagttc aagttcagaa ttgccttgga tacgcgtagt gttttttcag
    13141 gtgatccggg cgggattagc tcgcctgcgg ctcgtgatcc cgccctgccg taacgcaaaa
    13201 agtaaaggca gttcaagttc agaattgcct tggatacgcg tagtgtttnn nnnnnnnnnn
    13261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc catcgtgatc cgggcgggat tagctcgcct
    13381 gcggctcgtg atcccgccct gcctgaattg aggaggggag tggcttcccc atcgtgatcc
    13441 gggcgggatt cgaacccgcg acccacagct tagaaggctg ttgctctatc cagctgagct
    13501 accagacctt aacttttacg ggtgcaaatt tagacaattt atcgaaaaaa caggtacaac
    13561 cgctcaacta gctataaata ttaaccagac acgctgcagg atgtagtata ggtgcttaac
    13621 agatttcttc tcctgggcat ggtctgagcc tttactttac tggctattta ttctaaatgg
    13681 tctatttgac tttcagggat ttctttaagc tgaaatttca tgttgaaata tttaccagag
    13741 tatggatatg ggtcgttttc atctattcta tgttctcaac caggttaatt tggactacag
    13801 acaattttga gtaaagttta tagcatttgc caaacgtttg ttttatgcgc cgtgcgggat
    13861 tacgatgttg cgaacctatg tgccgtatta aacttcgaat cacactgttt gcaaattaaa
    13921 ctacctgctg cgattattgc atctgttgac tgctgttttg attattttgc ttgtcataca
    13981 gcctaaaaat ggttaaatta cccctatctc aagctgttta ctaagtttat ctaaattgaa
    14041 ttttgcggaa tttaaattac ctgatttttg aattggcata atttttttcc cattagaatc
    14101 ataaagaatc aatctatagt cattatcggc tatatcaaga gttcgattac tctggctgta
    14161 tgttcgccac aatttagtag atttttttat gccaattttc atgtcggatt gaataagaat
    14221 ccattttcca attggaaata acccgaaaat gtaaattgaa aatctgagtc tttttttccc
    14281 aaaatctatc gaggtaattg ttgacgtaaa tccaacgaaa gcacctatca gaactacaac
    14341 taatccagct attgaaaaat aggttataat tgagccagca acaaacagaa atatacctgc
    14401 cagggttcca accggaccaa acattttgtc aattttattt tttgttgtca ttttcaatct
    14461 attgtaagat attagcaggt tctacaaatt gctaccaaca gtggtggtat tgccagtaag
    14521 gtattgtggt gcgttttcct atccagctac acaactgatt aagcgggctt aaaactttcg
    14581 taaaaccact taaaccctta atggcaatta agcgtgttgt aggctgggtg tttttcaatt
    14641 caatcctgtc aaatttaatt gtttaatccg tttcactcgc tctctttgat aatccattat
    14701 atcagagtat agtttatctg accttaaaaa aatcaaatgg gtcaatgcac aagccatttt
    14761 ataaccatca gctatttcaa cggtgcacat tatcccagaa gtatctccac tgttataaac
    14821 acccgtaatt ttcaattctg tttttaatgt tattgggtgt ccatttttac gaaaaatctg
    14881 taccaactct cgaataggga atgcttttag
//
LOCUS       DAOPHX010000059        14597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_4768_length_14597_cov_5.939417, whole genome shotgun sequence.
ACCESSION   DAOPHX010000059 DAOPHX010000000
VERSION     DAOPHX010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 14597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14597
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4768_length_14597_cov_5.939417"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(727..1809)
                     /gene="wecB"
                     /locus_tag="PK502_05850"
     CDS             complement(727..1809)
                     /gene="wecB"
                     /locus_tag="PK502_05850"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPH00372.1"
                     /translation="MNSKLILTVLGARPQFIKAAAVSRQLMAFGLEEVIVHTGQHFDE
                     NMSEIFFKEMCIPAPKYNLEVHGLNHGAMTGRMMELLENIVINEKPAAVMVYGDTNST
                     LAGALVAKKLNTRVVHVEAGLRSFNMTMPEEINRILTDRISDLLFTPTEAADSNLMAE
                     GFSHFNAKIIRSGDVMQDAAYFYFNRAKTESHVLERLNLPEHFVLATFHRQENTDNLN
                     NLQGIVDGLNRINSETQVVVPLHPRTAKILERVSISPNFRIVEPLGYFDMLMLLNQSS
                     LVVTDSGGLQKEAFFFGKHCVTLREQTEWVELVEKGYNMLSGTSSDSIYNSFRQMIAR
                     KSNFKVNLYGNGHAAEKIAAELKDWL"
     gene            complement(1802..3118)
                     /locus_tag="PK502_05855"
     CDS             complement(1802..3118)
                     /locus_tag="PK502_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPH00373.1"
                     /translation="MEKKLRRRVLVITYYWPPSGGVGVQRWLKFVKYLRLYGWEPVVY
                     TPENPESFYDDESLLNDIPEGVEVIKTRIWEPYKIYKRITGTKNEKIAVGFTSSSNKK
                     KGLLHRVAVWLRGNLLIPDPRVFWVNPSVKFLKKYLRENPVDVVVTTGPPHSIHLIGL
                     KLKQKMKVKWLADFRDPWTNIYYIKDLRLSAPAMRIHRRLESKVVREADGVIVVSAQM
                     KNELMVLRPKRINLITNGFDEEDWPNEPVDLDSKFSITHVGTLSRYFNPVILWEVLSD
                     LCEEIEGFRNDLVIRLVGQVDATAIESIQKVNLQNNLESLGQVTHVEAVKFQRKSQVL
                     LLVVNSSPNAMGILTGKVFEYLASGRMVLAVGPTYGDLASLLSETKAGFFVDYNDKAK
                     TKRVITHLYNLYKENNLRPETKDILRYSRKELTRTLSNLLNEMTNE"
     gene            3579..4583
                     /gene="amrS"
                     /locus_tag="PK502_05860"
     CDS             3579..4583
                     /gene="amrS"
                     /locus_tag="PK502_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPH00374.1"
                     /translation="MEAKYYSKKGNGIRCNLCPHSCSIQDGRFGICKARQNQGGVLNA
                     LGYGVISSSGLDPMEKKPLYHFHPGSRIFSIGSYGCNLHCDFCQNFEISQSFPVSMPP
                     LRSYQPSEVVEKARVLPENIGIAYTYNEPTISFEFMLETARLAKSKGLLNVAVSNGYI
                     NPAPLRELLEVLDAFNIDLKSFSDDFYRSQTGGRLKPVLKTLVTIRELGKHLEIAFLV
                     IPGLNDSLAETKAMVSWIASNLGSNTVLHINRYYPAYKLKIPPTPEERLVELYEIARE
                     KLAYVYLGNLHGNEVGCNTICPSCSSTVIRRNGYHTVISALTREGRCSSCGYGPIVVM
                     "
     gene            complement(4656..5243)
                     /locus_tag="PK502_05865"
     CDS             complement(4656..5243)
                     /locus_tag="PK502_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPH00375.1"
                     /translation="MLKDLVLQNRSYRRFYQEKNVDRESLVELVELTRFCPSGRNAQP
                     LKYFISNSEPTNSHIFSNLAWAAYLKEWNGPEEGERPSAYILILEDQTIATSSQRDQG
                     IMAQTIMLGAAEKGLGGCIIASVKGHELAKILGIPDHLKIVLALALGYPKEHVVIEPM
                     PQNGNIEYWRDDKGVHHVPKRDLSELIWNKTVGKG"
     gene            complement(5329..5904)
                     /locus_tag="PK502_05870"
     CDS             complement(5329..5904)
                     /locus_tag="PK502_05870"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPH00376.1"
                     /translation="MKHELPQLPYSADALSPVISAKTIEFHYGKHHQAYVNNLNNLIV
                     GTPFENATLETIIKEAEGGIFNNGAQVWNHTFYFLTLSPNPKSEPSGKLGEQISLDFG
                     SFAEFKDKFEKASAGLFGSGWTWLVLNSKGQLEITQESNAGNPLRKGLKPLLACDVWE
                     HAYYLDYQNRRPDYLHSFWNIVDWKVVEGRF"
     gene            complement(5935..8379)
                     /locus_tag="PK502_05875"
     CDS             complement(5935..8379)
                     /locus_tag="PK502_05875"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPH00377.1"
                     /translation="MNRLFKYFTFLILAIPTGLPRMAAAMVNKATPDTLQINGNLNTV
                     RSLITSREFSAAAMLTKEALSNSRKINYIKGVAWSEYYLGMICFENSTLDSAIYWLKR
                     SENDFAAVRDYSARVKACNLLGIYYRQSDSNDSSLDCHTRALKIAMENNDSAGIAESH
                     YGIGMTLNQLGYYPETMEHFHAAISIREKLNDSSGVAAILNSMGILFRNQGDYANALD
                     FYVRALNIHEKLGNIQGLAYQHNNMGLIYRDIGDYGKALDHFNKSWALKVKINDSRGI
                     SNSIMNIGSVYLLQNKLDSALIFFDQAASIKEQIGDKSGIANINLFRGECYQKLGLYK
                     KALDFLESSRSDYHALKEPRGETEAIIQLASTYFNSGMHDKAFQLIDEAKNMVVTLKM
                     LDLQQKVFLNLYNFYIELGDYRKGLEYYQLYIAARDSVMGTRNLRKMLNVELQDEYSR
                     LIKEHFERKDQEILSKEQAQQSKTVLLYILSGAFIILLLFLVMFIRTLKNKQKINNML
                     IAQQAEVEHQKQELMNQRDEIDTQKEMVVYQRDRIITMLTDLAESIDYAKKIQQAILP
                     KDSLLQQLFTDYFIIYQPKEAVGGDFYWAGRFNGTICFAVADCTGHGVPGGFMSMLGV
                     SMLNDMATADSVQSPSLMLQSLRHNIVAALGQTSFDEDNHDGMDIAFCTFDIKSGILT
                     YAGANMPIIINSKGEIPPSERIQPYQNGLYELKPDRMPVALYDKMDSFSEIRVKLSPG
                     DAVYLFTDGYVDQFGGPKSKKFGHDAFRTLINSVKDLSFSEQKQIIWSTIERWKGETE
                     NQTDDILVMGLKLPDK"
     gene            complement(8526..9134)
                     /locus_tag="PK502_05880"
     CDS             complement(8526..9134)
                     /locus_tag="PK502_05880"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPH00378.1"
                     /translation="MSFQLPTLKYDYKALEPYIDAMTMEIHHSKHHATYTNNFNAALE
                     GSSLASLPVEEIFKDVSKHPVAIRNNGGGFYNHSLFWDIMSPNGGGEPTGELLKAIQK
                     SFGSFSEFKEKFSSTATGRFGSGWAWLVLSKGELKIYSSPNQDNPLMDISEVKGYPIL
                     NLDVWEHAYYLKYQNRRPEYIQNWWNVVNWSAVEKRFLEGLK"
     gene            9412..11283
                     /locus_tag="PK502_05885"
     CDS             9412..11283
                     /locus_tag="PK502_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPH00379.1"
                     /translation="MASIYLQAENLTKSFGDRILFGNLSFSLFQLDRTAIVAKNGSGK
                     STLLSILAGKQHPDSGSVVTRNGIRLGYLEQNPVLRDDYTILESVYSADNDISRAVQD
                     YERAMATSDKDHLQQAIETMDRLEVWGRETKAKQILTRLRIENLNQPIANLSGGQRKR
                     VALASVLIEEPDILILDEPTNHLDLDMVEWLEDYLIHGQQTLLMVTHDRYFLDRVCGC
                     ILEIDNQSLFRYKGNYSYFVEKRQDRINALASEVEKANNTLRYESEWIHRMPKARGTK
                     SKYRIDAFYKLREKTLLQNLESPMEINISASRLGKKVMEVKNISKAFGSKVILRDFSY
                     TFNRFEKLGIVGRNGTGKTTFLNIITGNTLPDSGLVDIGSTVAIGYYKQQDVSFSNNA
                     RVIDIARDIAEVVTIADGKTITVSQFLNMFLFPPEKQHDFVQKLSGGEKRRLYLLTVL
                     MRSPNFLILDEPTNDLDIQTLCILEEYLKSFPGVVVVVSHDRFFMDKVVDGLLIFEGA
                     GEVSGFPGSYTELREWTLEQQNDCPQGKPSQKSVKTGSSRVRIKQRLSFAQKKELEHL
                     TSEIELLEREKLELEQQLSSGMLDASKLQEKSEQYGHLLERLGNIEMRWLELSELEG"
     gene            11581..12378
                     /locus_tag="PK502_05890"
     CDS             11581..12378
                     /locus_tag="PK502_05890"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPH00380.1"
                     /translation="MVNFRNRIAWVTGASSGIGKEIALALAREQAHLILSAKNPEELE
                     VVASECRKYTPFCHEVPFDLSDPEEVERIAKSVVNTYGHIFLLINNGGISQRSLAVET
                     PVEIDRKIMEVDYFSHVIITKTVIPGMIEAGVGYIAATSSISGKFGFMYRSAYAAAKH
                     AIQGFFETLRVELKPYNISVTIAYPGRIRTSISLHALDKEGNEYGKMDPGQSNGLAPQ
                     KCAKRYIRAVKRRKPEVLIGKSELLMVHIKRLLPSLFYKMVSRVKPV"
     gene            12391..13467
                     /locus_tag="PK502_05895"
     CDS             12391..13467
                     /locus_tag="PK502_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPH00381.1"
                     /translation="MEKIIYGIQQVGLGVSNLEEAWKWYINLLGFDVKIFDDDTVAEL
                     MLPYTGGEPQQRHAILALNLQGGGGLEIWQYKERTPLPPKFPVKLGDLGIFAAKIKSP
                     DIEAAFNFLKSKGVILGDLVNSPEGKPHFYISDPFGNTLEVYEDKSTFLNEGRSTSGI
                     GGAIVGVSQMERSVKFYSRVLGYDKVVYNKSGRFDDLAGLPAGNENFHRVLLTHSDTR
                     KGSFSELFGQSYIELIQPLDRVPLKILQDRFWGDLGFIHLCFDVKNMDQLERECNELG
                     FPFTVNSNIKHSDKGSFDMGEAAGHFTYIEDPDETLIEFVETHFLPIYKPLGIKVNFQ
                     NRRPYKSVPRWMIKLLRFKRVKKI"
     gene            13963..>14597
                     /locus_tag="PK502_05900"
     CDS             13963..>14597
                     /locus_tag="PK502_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPH00382.1"
                     /translation="MTPKDYMDRESKYGAHNYHPLPVVLEKGKGVYLWDVEGKKYYDF
                     LSAYSAVNQGHCHPKIVKALTDQAQLLTLTSRAFYNNVLGEFEEYVTKYFGYDKVLPM
                     NTGAEGDETALKLCRRWAYDVKGIKDGEAKIIVCENNFHGRTITVISMSTDPDAYRGY
                     GPYTPGFIKIPFNNIPALEDALKDKNVAGFLVEPIQGEAGVFVPDDGYLKKA"
BASE COUNT         3947 a         3119 c         3319 g         4212 t
ORIGIN      
        1 gttccgcccc cgacccattt gcgggtctgg cttttatgcc gtttttctaa tgccagcaac
       61 gttcaacgtt tcccgccttt ccgggcaccg cggagaatac cttttcccag ttggggctga
      121 agacaaggat aaaggttaaa ggataaagga taaaaaattg ccttgctaac gacggaactt
      181 gtccggctcc tgacggaact tgtccgcctc ctggcggatt cacctaacat taaaaagggt
      241 tttaaagagc ggcatcggcc ggcgagccga aaaacaagcg caggcggcgt agaaaaaatc
      301 tgctgttcga agcgctgagc ggagcgaggc gcaagttcag attttttagc cgccaagcgt
      361 agctttttcg tgcgaggcgg gcgcagcctt gacttttttt gcatactttt ttgtgtcaag
      421 acaaaaaagt atgtggggtt tggggcaaag ccccaagatt tcatgataaa aacttatcca
      481 gcttttaaca aactcacaaa ttcattctgc acctttatgg cttaacactt ctctccggaa
      541 cgccaaaagg aactttgccc catgctagct attgcgtccc gttggggctg aagaaaaagt
      601 aaggtaatct tgcccggctt tttaccttta acgcttcaca atgcacatct gctcttctgg
      661 attttatttt cttcctcttt gcttcaaaat gggttgtaag agtttttatt gaatctgggt
      721 tttagcctaa agccaatcct ttagctctgc tgcaattttc tccgctgcat gcccattccc
      781 gtaaaggtta accttaaagt ttgattttcg ggcaatcatt tggcggaagg agttgtatat
      841 gctgtcggag ctggttcctg aaagcatgtt gtagcccttc tcaaccagct caacccattc
      901 ggtttgctca cgtagggtta cgcaatgctt tccaaagaaa aatgcctctt tctgtaaacc
      961 gccgctatcg gtaaccacca gcgaggattg gtttagcagc atgagcatat caaaatagcc
     1021 taagggttca actatcctaa agttgggtga aatacttacc ctttcaagaa tttttgctgt
     1081 tcggggatgt aggggaacaa ctacctgagt ttcagaattt atacggttca gcccatcaac
     1141 tattccctga agattattaa gattatcggt gttttcctgt ctgtgaaagg tagccagcac
     1201 aaagtgctcg ggtagattta agcgttcgag tacatgtgac tcggttttag ccctgttaaa
     1261 gtaaaagtag gctgcatcct gcataacgtc gccactcctg ataattttgg cgttgaaatg
     1321 gctgaatcct tcggccatca ggttgctgtc cgccgcttct gttggggtaa aaagtaaatc
     1381 cgatatccgg tcagtaagaa tgcggttgat ctcctcgggc atggtcatgt tgaacgagcg
     1441 gagtcctgct tcaacgtgca ccacgcgggt gttcagcttt ttggctacca gtgctccggc
     1501 aagtgttgaa ttggtgtcgc cataaaccat tactgctgcc ggtttttcat taataacgat
     1561 gttttcgagc agctccatca ttctaccagt catggcgcca tggttcaaac catgaacctc
     1621 aaggttgtac tttggagcgg gaatgcacat ttccttaaaa aatatctcgg acatgttttc
     1681 atcgaaatgc tggccggtgt gcacaatcac ttcttccaat ccaaaagcca taagttggcg
     1741 gctaactgcg gctgccttta tgaactgggg tctagcccct aaaacagtaa gtatcaactt
     1801 gctattcatt agtcatttca tttaataggt ttgataaggt gcgggtgagc tccttacggg
     1861 agtaacgcaa aatatccttg gtctctggcc gcaggttgtt ctctttatag agattgtaga
     1921 gatgtgtaat gaccctttta gtttttgctt tatcgttata gtccacaaag aatcctgctt
     1981 ttgtctcgga aagcagggag gccaaatcgc cataggtggg tccaactgcc agcaccattc
     2041 gccccgaggc taagtactcg aatacctttc cggtaagaat acccatagca tttggagaag
     2101 aatttacaac caggagtaac acctgcgatt ttcgttgaaa ttttaccgcc tcgacatggg
     2161 taacctgtcc caaactttca aggttatttt gaaggtttac tttttggatg gattctatag
     2221 cagttgcatc tacctgtcct acgagcctga taaccagatc atttctaaaa ccttcaattt
     2281 cttcacataa atcggagagt acctcccata atatgacagg gttaaagtaa cgggatagcg
     2341 taccaacatg ggtaatcgag aattttgagt ccaggtcaac cggctcattt ggccaatctt
     2401 cctcatcaaa gccattggta atcagattta tacgtttagg tctgagaacc ataagttcat
     2461 ttttcatctg cgccgataca actatcaccc catcggcctc acgaaccacc ttgctttcca
     2521 acctgcgatg gattcgcatt gcaggagcgc ttagtcttaa atccttaatg tagtaaatat
     2581 ttgtccatgg atccctaaaa tcggctagcc attttacttt catcttttgt ttcagcttca
     2641 gcccgattag atggatggag tgcggtgggc cggttgtaac cactacatcc accggatttt
     2701 cccttaggta tttttttaag aacttaaccg atgggttaac ccagaagact cttggatcag
     2761 gaataagcag gttgcccctt agccagacag ccaccctatg cagtaatccc ttttttttat
     2821 ttgaagaaga ggtaaaaccc acggcaattt tttcgttctt tgtgccggtg attcttttgt
     2881 atattttgta gggctcccat attctggttt ttataacctc aaccccttca ggaatgtcat
     2941 tcagcagcga ttcatcgtcg taaaacgatt cgggattttc gggtgtgtaa accaccggtt
     3001 cccatccgta cagcctgagg tactttacaa actttagcca acgttgaacg cctacaccac
     3061 ctgatggggg ccagtagtag gtgataacca gaacacgcct gcgtaatttt ttctccattt
     3121 attaaataaa agttcaaagg taaaaatttg gccgcaaccc ggaaaggaat agtagtggaa
     3181 gttccctaat ttcagattaa tctctttaat gtttttactt tacagcggct tcatgcagtc
     3241 ctttactact atgacctaca tgtaaatacg ggagttattt aatcgtcaac attatcatgc
     3301 aaatcctaaa tttaatttgt gttggtcaag ctatgcagga agcaggtaaa cttcaaaatt
     3361 tctgatatcc aacaggtgta aaacatacag gtactatttt taatgatttt tgtgggatga
     3421 tttctgcgtt gaagaagtga aaaagcattc aacactcgat tttttaaatt ttggtttaac
     3481 ctataggtat ggtatcgttg gaattaagtt gggatgactt gcaattaaag caaaagttta
     3541 tcgaactttg atgataattc taaatgtgct ttttgatcat ggaggcaaag tactattcaa
     3601 aaaagggtaa cggcataagg tgtaaccttt gccctcatag ttgtagcata caggatggaa
     3661 ggtttggtat atgcaaggca cgtcaaaacc agggtggggt tctcaacgct ctaggatacg
     3721 gggtaatttc gtcatcgggg ctagacccaa tggaaaaaaa gccgctttac cactttcatc
     3781 cgggtagccg gatattttct atcggcagct acgggtgcaa cctgcactgc gatttttgcc
     3841 agaacttcga gatttcgcaa tctttccctg tttcgatgcc cccgttgagg agttatcagc
     3901 cttcagaagt tgttgagaag gccagggttc ttccggaaaa cattgggatt gcatatacct
     3961 acaacgaacc aacaatatca tttgagttta tgcttgaaac ggcaaggttg gctaaaagta
     4021 aaggtttgct gaatgttgcg gtttcaaatg ggtatatcaa ccctgcacca ttgagggagc
     4081 ttcttgaagt tctggatgct ttcaatatcg atttaaaatc attttccgat gatttttacc
     4141 gcagtcaaac aggaggaagg ctcaagcccg tgctgaaaac tctggtgacc ataagggaat
     4201 tgggtaaaca tttggagatt gcctttctgg tcattccggg actaaacgat tcgcttgccg
     4261 aaaccaaggc aatggtttcg tggattgctt cgaatctggg aagcaacacg gttttgcata
     4321 tcaaccgcta ctaccctgcc tacaagctga aaattccgcc tactcccgaa gaaaggttgg
     4381 ttgagctata cgaaatagct cgagagaagc tagcctatgt gtacttgggt aacctgcatg
     4441 gaaacgaggt gggctgcaac accatttgtc cgtcgtgttc ctccacggtt atccgtagga
     4501 atggctatca caccgttatt tccgccctaa cccgtgaggg taggtgcagc agttgtggct
     4561 acggaccaat tgttgtaatg taaggctgct aagctattcc tgttgaagaa tagcgttgga
     4621 ttacctggct ttggcgatta gaagaatgta attgtttaac ctttaccgac tgtcttgttc
     4681 caaatcagct cgctaaggtc acgttttgga acatgatgta cacccttatc atcccgccag
     4741 tactctatat ttccattttg aggcattggc tcaatcacaa catgttcttt tgggtagcct
     4801 aacgcaagtg ccagcacaat tttgaggtgg tcgggaatac ccagtatttt agccaattca
     4861 tgacctttaa ccgatgcaat gatacaacct ccaagcccct tttctgcagc accaagcatg
     4921 atggtttgag ccattattcc ctgatctctc tggctggacg tggcaatggt ttgatcctca
     4981 agtatcagga tgtaggctga tggtctttct ccctcttcgg ggccattcca ttcctttaga
     5041 tatgcagccc atgccaggtt ggaaaatata tggctgtttg ttggttcgga atttgaaatg
     5101 aagtacttta gaggttgtgc gttacgaccc gatgggcaaa acctggtcag ctcaaccagc
     5161 tcaaccagcg attctctgtc aacatttttt tcctggtaga acctacgata gctgcggttc
     5221 tgcaacacca aatcttttaa catggtttta attttttaca aattattgga taaaagaaat
     5281 acccggtaag cttaattcct accaggtaag tttaaaaaaa tcttttagct aaaaccttcc
     5341 ctctacaact ttccaatcaa ctatattcca gaagctatga aggtaatcgg gccggcgatt
     5401 ctggtaatcg aggtagtagg cgtgttccca tacatcacac gccaaaagag gctttaggcc
     5461 tttgcgaagg ggatttcccg cgtttgactc ctgggtaatc tccagctggc cctttgagtt
     5521 taaaaccaac caggtccatc cggaaccaaa caggccggct gaagcctttt caaatttatc
     5581 tttaaattct gcaaatgagc cgaagtctaa agaaatctgc tctccgagtt ttcctgacgg
     5641 ttcgcttttt ggattgggcg aaagagtgag gaagtagaag gtgtgattcc aaacttgtgc
     5701 accgttgttg aaaattccac cctctgcctc cttgatgata gtctcaagtg tggcgttctc
     5761 aaacggtgtc ccaacaataa ggttgttgag attgtttacg taagcctggt ggtgctttcc
     5821 gtaatggaac tcaatcgttt tggcagaaat taccgggctc aaggcatcgg ctgagtacgg
     5881 tagctgcggc agttcatgtt tcatcttggt taattttaaa ggttacactg ttttttattt
     5941 gtcggggagc tttagtccca taaccaggat gtcatcggtt tggttctcgg tttctccctt
     6001 ccagcgctcg atggtactcc aaatgatttg cttctgctcg gaaaagctta aatctttcac
     6061 tgagttaata agtgtacgga aggcgtcatg tccaaacttc ttactttttg gcccaccaaa
     6121 ctggtcaaca tatccatcgg taaaaaggta aacggcatct cctggcgata gcttgacgcg
     6181 aatttcgcta aaggagtcca tcttatcgta aagtgcaact ggcattcggt ctggttttag
     6241 ttcatagagc ccattttgat agggctgaat acgttcggag ggtggtattt cgcctttaga
     6301 gttaataatg ataggcatgt tggctcccgc gtaagttaaa atacccgact tgatgtcgaa
     6361 ggtgcagaag gcaatgtcca tcccatcatg gttatcctca tcaaagcttg tttgcccaag
     6421 ggcagccaca atattgtgcc tgaggctttg cagcattaat gatggggatt gaacgctgtc
     6481 ggcagtagcc atatcgttaa gcattgaaac tcccagcatg ctcataaagc ctccagggac
     6541 gccatgtccg gtacaatcgg ctactgcaaa gcaaatggtg ccgttgaacc ggccggccca
     6601 gtagaaatcg cccccaacag cctccttagg ctggtatatt atgaagtaat cggtaaatag
     6661 ctgctgcagg agggaatctt ttggtagaat ggcttgctga attttttttg catagtcaat
     6721 tgattctgcc aaatcggtga gcatggtaat aatcctatca cgctggtaaa ccaccatttc
     6781 tttctgggtg tcaatctcgt cgcgctggtt catcagctcc tgtttttgat gctccacctc
     6841 cgcctgctgg gctataagca tgttatttat tttttgttta ttttttaagg tgcggatgaa
     6901 cattaccagg aatagcagaa gaatgataaa tgcccccgaa agtatataga gtaaaactgt
     6961 tttgctttgt tgtgcctgtt ctttagacaa aatctcctga tcttttcgtt caaagtgttc
     7021 tttaataagt cgggagtact catcttgcag ctcaacatta agcattttcc ttaagtttcg
     7081 tgtacccatc acactatcgc gggcggcaat gtatagctgg tagtattcca gtccttttct
     7141 ataatctccg agttcgatat aaaaattata gaggttcagg aaaactttct gctgcaggtc
     7201 gagcattttc aatgttacta ccatattttt agcttcatcg atcagctgaa aggccttgtc
     7261 gtgcataccc gagttaaagt atgttgaagc cagctggatg attgcttcgg tttcaccccg
     7321 gggttctttt agagcatggt agtcacttct ggatgattcg agaaagtcta aagctttttt
     7381 gtaaagacca agtttttggt agcattcacc cctgaataaa ttaatattgg caattccact
     7441 cttatcacct atctgctcct ttatggaggc ggcctgatca aaaaatatta gtgcgctgtc
     7501 cagcttgttc tgtaaaaggt aaaccgaacc aatattcata attgagttgg aaattccccg
     7561 gctatcgttt attttaactt tcaaggccca tgatttgttg aaatgatcga gtgccttacc
     7621 gtaatcgcca atatccctgt aaattagacc catgttgttg tgctggtagg ctaatccctg
     7681 aatatttccc aacttctcat gaatgttcaa tgcccggaca tagaaatcta acgcattggc
     7741 ataatcgccc tgatttctaa agaggatgcc catgctattc agaattgccg ctactcctga
     7801 gctgtcattt agcttttccc ttatcgagat ggctgcgtga aaatgctcca tggtttcggg
     7861 gtaatagccg agctggttga gggtcatacc aattccatag tgcgactcgg caataccggc
     7921 cgagtcgtta ttttccatgg caatctttaa agctcgggtg tggcaatcca gggagctgtc
     7981 gttgctatcg gattggcgat agtaaattcc cagcaggttg catgctttta cccgtgcact
     8041 atagtcgcga acggcagcaa aatcattttc tgatcgtttt agccagtata ttgcagaatc
     8101 tagtgttgaa ttctcaaaac atatcattcc caggtagtac tcactccatg caacaccttt
     8161 aatgtagttg atttttcttg aattgctgag ggcttctttg gtgagcattg cagctgctga
     8221 aaactcccgg gaggttatta gcgacctaac cgtattcaga tttccattaa tttgcagggt
     8281 gtccggtgta gccttattta ccatggctgc agccattctt ggcaagcccg tggggatagc
     8341 taaaattaaa aaggtaaagt atttgaaaag tctattcatc agcagcttat tataggcaga
     8401 atagcatatt ttataagctt aaatttacaa aagtataacc aaaataaaaa cagcagtaca
     8461 aaaaagaggc tgggaaattg tcccagcctc aaaaccaaat aacaaaacaa aatgaccctg
     8521 gcaggttatt ttaagccttc taaaaatctt ttttcaacgg ccgaccagtt caccacattc
     8581 caccagttct gaatgtattc ggggcgacga ttctggtatt taagatagta ggcatgttcc
     8641 catacatcga ggttgagaat ggggtagcct tttacctccg atatatccat cagggggtta
     8701 tcctggttgg gagaggagta tatctttaat tctccttttg atagcacaag ccaagcccag
     8761 cctgacccaa accttccggt tgcagtggat gaaaactttt ccttaaactc gctgaacgat
     8821 ccaaaacttt tctggattgc cttaagcagc tcaccggtag gctctccacc cccattggga
     8881 gacatgatgt cccaaaacag gctgtggttg taaaatcccc cgccgttgtt acgaatggca
     8941 accggatgct tggaaacatc cttaaaaatt tcctcaacgg gaagtgaggc cagcgagcta
     9001 ccttccaggg cagcattaaa gttgttggtg taggttgcat gatgctttga gtggtgaatc
     9061 tccattgtca tggcgtcaat ataaggctca agagccttat aatcatactt tagtgttggt
     9121 agttgaaatg acatagcttt atcgtattat gttatttatt ctcattctaa atagagtaac
     9181 caacatgtag caatattgtt cagcgttgtt ggaaattttc tatgtttaag acaagataca
     9241 tgaaacggat ttcgacctgc agctgatttg ctgattcgtc aaggggttgt tgcttgtaaa
     9301 tacttaaaaa acttgagagg tgtataagca tcagccttca acctgccagc agcatgttgt
     9361 ttgtatttag ccatttatac ttaactttgc gggtcaaatt caactatttt tgtggctagc
     9421 atataccttc aggctgaaaa ccttacaaaa tcttttggtg atagaattct cttcggtaac
     9481 ttgtcgttta gcctattcca gctcgataga acggctattg tagcaaagaa cggttccgga
     9541 aagtcaacgc ttctgagtat tctggcagga aaacagcacc ccgattcggg cagtgttgtt
     9601 acaagaaacg gaataaggct gggctacctc gagcaaaatc cggtgttaag ggatgattat
     9661 acaattctgg aaagtgttta tagtgccgat aatgatattt ctagggcagt gcaggattac
     9721 gagagggcga tggcaaccag cgataaggat cacctgcaac aggctatcga aacaatggac
     9781 aggttggaag tatggggaag ggaaaccaag gcaaagcaaa ttttaacccg gctacgaatc
     9841 gaaaacctta atcagccaat tgccaatctt tctggtgggc aacgcaagcg ggtagctctt
     9901 gcatcagtcc tcattgaaga acctgatatt cttatactcg acgaacctac caaccatctt
     9961 gatttggata tggttgagtg gctggaggac tatttaattc acgggcagca aacccttttg
    10021 atggttacgc acgacaggta ctttcttgac agggtttgcg gttgtattct cgagattgac
    10081 aaccagtcct tgttccggta caaaggaaac tactcctact ttgtggaaaa acggcaggat
    10141 aggattaacg cccttgcttc agaggttgaa aaagccaata acacgctaag atacgagagt
    10201 gaatggatac accgaatgcc aaaggccagg ggcacaaagt ccaagtatcg tattgatgct
    10261 ttttacaagt taagggaaaa aaccttgctg caaaacttgg aatcaccgat ggaaattaac
    10321 atcagtgcct cccgattagg gaaaaaggtt atggaagtta aaaacatatc taaggctttt
    10381 ggaagcaagg ttattctcag ggatttcagc tatacattta acaggtttga gaagctggga
    10441 attgttggta gaaatggaac cggtaaaaca acttttctca acattatcac aggtaatact
    10501 cttcccgata gcgggttggt tgacattggc agcacggtag ctatcggcta ctataagcag
    10561 caggatgtta gcttttccaa caatgccagg gtaatagaca ttgccagaga tattgccgag
    10621 gtggttacca ttgccgatgg caaaacaatt accgtttcgc aatttctcaa catgttcctt
    10681 ttccctcccg aaaagcagca cgattttgtc caaaaactaa gcggagggga gaaacgcagg
    10741 ttatatcttt taaccgttct tatgcgtagc cctaactttc ttatcctcga tgaacccacc
    10801 aacgacctcg acattcaaac cctttgcatc cttgaagagt atcttaaaag ctttcccgga
    10861 gttgtagttg tggtttctca cgaccgtttt tttatggata aggttgttga tggcctgctg
    10921 atatttgagg gcgccggcga ggtaagcggt ttccccggat cgtacaccga attgagggaa
    10981 tggactcttg aacagcagaa tgattgcccg cagggcaaac cttctcaaaa atcggtaaag
    11041 accgggagta gcagggtgag gattaagcag cggctttcat ttgcccaaaa aaaggagcta
    11101 gaacacctaa cctccgaaat cgagctgctt gaaagggaaa agctagagct ggagcagcag
    11161 ttgagtagcg gaatgctgga tgccagcaaa ctgcaggaga aatcggaaca gtatggacat
    11221 ctactcgaaa ggctgggaaa tattgagatg cgatggttag agttaagcga gcttgaaggt
    11281 taggtagcca cattaaagta ccaacctctt ggttgaaatt actggataat agttgttttt
    11341 agactggaaa atcaggtggg agaaagcaag caggtaaacg tttcatgttt atttttgtgt
    11401 gagcattggg aaatagcagg aatgaagtta atgtaaaaat ttctcaaaat ggaacatttg
    11461 agtacggctg tgctttggat tgcctcccct gcttcctttt attcaaatgt aaaaagagct
    11521 aaatttgcca ctcaaaattt ctgaagggtt tctgaaaccc gttagagaag aacacatacc
    11581 atggtaaact ttagaaatcg cattgcttgg gtaacaggag cctcgtcagg cataggcaag
    11641 gagattgccc tggcacttgc aagggaacag gcacatctta tactgtcagc caaaaatcct
    11701 gaggagcttg aggttgtggc atccgaatgc cgcaagtaca cccctttttg ccatgaggtt
    11761 ccttttgact tatctgatcc tgaagaagtt gagcgcattg caaaatctgt ggttaacacc
    11821 tatggtcata ttttcctgct gattaataat ggtggaatta gccaacgctc gttggctgtt
    11881 gaaacccccg ttgaaataga ccgaaaaatt atggaggtcg attacttttc tcatgtcatt
    11941 attactaaaa ctgttatacc aggaatgatt gaagcgggtg tggggtatat tgcagcaacg
    12001 agcagtattt caggaaaatt cggattcatg tatcgttcag cttatgctgc agccaaacac
    12061 gccattcagg gtttttttga aacgcttagg gtagagctta agccttacaa catttcggta
    12121 actattgcct acccgggccg aatccggacc agtatttccc ttcatgccct ggataaggag
    12181 ggtaacgagt atggaaaaat ggaccccggg cagagcaacg gcctggcacc gcaaaaatgt
    12241 gccaaaaggt atatccgtgc tgtaaagcgg cgtaaaccgg aggttttgat tggaaaaagc
    12301 gagctgctga tggttcacat taaacgttta ctgccatcgc tattctataa aatggttagc
    12361 agggtaaaac ctgtgtaaaa gttaacattc atggagaaaa ttatttacgg cattcagcag
    12421 gttggcctgg gggtgagcaa cctggaagaa gcatggaaat ggtacatcaa cttgttggga
    12481 tttgacgtta aaatttttga cgacgacacg gtagccgagc taatgctccc ctacactggt
    12541 ggtgaacccc agcagcggca cgcaattctt gccctgaacc tccagggagg cggtggcttg
    12601 gagatttggc agtacaagga gcggactcct cttcctccaa agttcccggt aaaacttggc
    12661 gacctgggaa tttttgctgc taaaattaaa agtcccgata tcgaggctgc ctttaacttt
    12721 ctgaaatcca aaggcgtaat acttggcgat ttggttaatt ctcctgaggg gaagccacat
    12781 ttctacatat ccgacccctt tggcaatacc ctggaggttt acgaggacaa atcaaccttt
    12841 ttaaatgagg gaagaagcac ttccgggatt ggcggagcca ttgttggggt aagccagatg
    12901 gagaggtcgg taaaatttta ctcgagagtg ctgggctacg acaaggttgt ttacaataaa
    12961 tcaggcaggt tcgatgacct tgccggcctt cctgccggaa acgagaattt tcaccgggtg
    13021 ctacttaccc attcggatac ccgcaagggc agctttagcg agctatttgg ccaatcgtac
    13081 attgagctta ttcaaccgct ggatagagtt ccattgaaaa tcctgcagga ccgtttctgg
    13141 ggtgaccttg gctttattca cctttgcttt gatgtaaaga acatggacca gctcgaacgg
    13201 gagtgtaacg agctgggttt tcccttcact gtcaactcaa atatcaagca tagcgataag
    13261 ggtagttttg atatgggcga ggctgcaggc catttcacct acattgagga tcctgacgaa
    13321 actctgattg agtttgtcga aacccacttc ctgccaatat ataaaccgct aggtataaag
    13381 gtgaattttc aaaaccgcag gccatacaag tctgttcccc gatggatgat taagctgctt
    13441 cgttttaaaa gggtcaagaa gatttaatgg ctgtctattg ttgcaaccat ttttcccaaa
    13501 ctacacctta aaccctaaat gattaccatg aacttggggt gcagggttta accttaaccc
    13561 aataggtttt agctatcacg ctggcttaaa tatgtttcaa ggggacaccg gtagagcaaa
    13621 tgaaatatcc ttttatggat ttttttagct gtgattaacc agtagattat gcgtagttgc
    13681 gcaatcagta tgttgaattt cagtgtttta gagtgctgaa gtagaaagtt aacggtatcc
    13741 ccttgcacga gcgttcgctg ctttactgtt tagcttaaac acaaaacgcc aggattttta
    13801 ttcctgctat agagggtaga attatgttcc ttaacatatt ccacccaagg gcaggctatt
    13861 tgaaaattga caaactagta gtaaccttgc ataccttaaa aaagacaatg atttttaagc
    13921 aaaatattgt ttaacccgta attcatagta aaaatgagta agatgacacc aaaagattac
    13981 atggatcgtg agagcaagta cggtgctcac aactaccatc cgcttccggt tgttttggaa
    14041 aaaggtaagg gggtttacct ttgggacgtg gagggaaaga agtactacga ttttctttcg
    14101 gcctactctg ctgttaacca ggggcattgc cacccaaaaa ttgttaaagc gttaaccgac
    14161 caggcccagc tgcttaccct tacctcaagg gctttctaca acaacgtcct cggtgagttt
    14221 gaggagtacg ttaccaaata ctttggctat gacaaggtac tgccgatgaa tacaggggct
    14281 gaaggcgatg aaacagctct caaactttgc cgccgttggg catacgatgt gaagggaata
    14341 aaggatggcg aggccaagat tattgtttgc gagaataact tccatggccg cacgataacc
    14401 gtgatttcca tgtcaaccga tcccgatgcc taccgcgggt acggccccta cacccccggt
    14461 tttattaaaa ttccgttcaa caacatacct gcccttgagg atgcccttaa ggataaaaac
    14521 gttgccggat tcctggtaga gccaatacag ggcgaagcgg gagtgtttgt tcctgacgat
    14581 ggttacctca aaaaagc
//
LOCUS       DAOPHX010000060        14224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_4953_length_14224_cov_6.370527, whole genome shotgun sequence.
ACCESSION   DAOPHX010000060 DAOPHX010000000
VERSION     DAOPHX010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 14224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14224
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4953_length_14224_cov_6.370527"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /gene="ftsH"
                     /locus_tag="PK502_05905"
     CDS             <1..1619
                     /gene="ftsH"
                     /locus_tag="PK502_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPH00383.1"
                     /translation="YPEYTDQHNYWGDILSWVIPILLIVGLWAFFFRRMTKNAPGGAA
                     GPGNIFSVGKSKAQLFDKESNQKIDFKDVAGLEEAKVEVMEIVDFLKNPKKYTELGGK
                     IPKGVLLVGPPGTGKTLLAKAVAGEANVPFFSMSGSDFVEMFVGVGASRVRDLFKQAK
                     EKSPCIIFIDEIDAIGRARGRNANFSGNDERENTLNQLLTEMDGFASNQGVIIIAATN
                     RADVLDKALLRAGRFDRQIHVELPDYKERLDIFKVHLRPLKLEGNVDITFLAKQTPGF
                     SGADIANVCNEAALIAARKNKKQVERQDFLDAIDRIIGGLEKKNKIISKDEKRAIAYH
                     EAGHATVSWLLEYANPLVKVSIIPRGISLGAAWYLPEERQITTTEQLLDEMCATLGGR
                     AAEELVFGKISTGALNDLERVTKQAYAMITYFGMGDNFRNISYYDSTGQSEFTFSKPY
                     SEKTAEQIDEEVKAIIDKAYNRAKEILRENSDGHVKLAELLLEREVIFSEDLEKIFGP
                     RKSLSREEELAKEFSDELKKTQSPNESDNG"
     gene            1612..1815
                     /locus_tag="PK502_05910"
     CDS             1612..1815
                     /locus_tag="PK502_05910"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HPH00384.1"
                     /translation="MDDSWIKVFSTSLPWQAEMAKQVLEENGIEAVVINQQDSSFFFG
                     EAIVYVEGKWEDQAKELLKGIGS"
     gene            1812..2639
                     /locus_tag="PK502_05915"
     CDS             1812..2639
                     /locus_tag="PK502_05915"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPH00385.1"
                     /translation="MTEFLKRTISGLIFVVTITGALVIGHVAFFIVFLGLMVATQYEF
                     YKMTFKTHLRPQILLGLFIGIALFSWSFLYSLGKVEQITIFGFIPLLGGIFVVELYRN
                     QHKPIQNIAVTLLGIIYIAVPLSLVNFIVINGSSTRISYSPNILLGLLFLIWSNDTGA
                     YLLGVSMGKHKLFPRISPKKTWEGFVGGLLLTMIVSWVDAIIFPEVAFKHWLVIGVIT
                     AIVGAFGDLVESMFKRSVGVKDSGKFLPSHGGFLDRFDAFLLVIPFVYAYLEVMMLI"
     gene            2652..3299
                     /locus_tag="PK502_05920"
     CDS             2652..3299
                     /locus_tag="PK502_05920"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HPH00386.1"
                     /translation="MKIHREGLVFLAVSLIILAALNVAVGLILPEVTYFLAPLSLLIL
                     LFLVRFFRIPMRGFSPNNEYIYSPADGTVVAIEEVEENEYLKTRCIQVSVFMSIWNVH
                     INWFPTTGTIKYYKYHPGDYLVAWHPKSSSHNERTTVVVERSDGTKILIRQIAGALAR
                     RIVCYAKPGQSIEQGSELGFIKFGSRVDLFLPLNANVEVKLNDRVVGKQTVIARL"
     gene            complement(3305..3826)
                     /locus_tag="PK502_05925"
     CDS             complement(3305..3826)
                     /locus_tag="PK502_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00387.1"
                     /translation="MNTALKIACYTVLLLVAGNSCNSVSKVTTTNQGISKQEFLKAPF
                     GHDESIQAFTTQLPARTKITRFARSNAHEPSRTDTIYKFSYKKSELFVYKTYFNREML
                     MAGKIVNPEIKLVNGISVGLTRDQLLKAISNINESSSDTLVISNEYYGRTFNFIFNNK
                     GKVREIVFSSYAD"
     gene            complement(3892..4815)
                     /locus_tag="PK502_05930"
     CDS             complement(3892..4815)
                     /locus_tag="PK502_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPH00388.1"
                     /translation="MDFISSHTGELAALLTAVFWTVTSLAFESASKTVGSLSVNFIRL
                     VLAFIFLSLYSLFARGLLLPTDAGGHQWLWLSLSGLVGFVMGDLFLFQSYVLVGARIS
                     MLIMSLSPPIAAGIGWLSMGETLTLKQALGMLLIFIGIAMVVLRSEKTPNGSKIIGRK
                     IKFGYSIPGLLLAFGGAVGQAGGLVLSKYGMAGYDVVASVQIRVITGIVGFAAVLLFM
                     HKSGNLLVAVKNAGAMRRITVGSFFGPFLGVSFSLLAVRYTSTGVASALMSIVPVLII
                     APSAVLLKEKITFREVAGAIVSVVGVVTFFL"
     gene            5017..6120
                     /locus_tag="PK502_05935"
     CDS             5017..6120
                     /locus_tag="PK502_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPH00389.1"
                     /translation="MNKLVKYIIIVFVVAAVAFLLWYFSSIVAYILISAVLSLMGKPI
                     VDGVCRLKIRNWHPPKAVGATCALIVIWVLFVAFFSFMIPLVVSQFNSLSGVDVGTLV
                     NRFAHPIAGLQEFIQNYLPASAKEFSIEEYLTSKISTFFTTSMIANLFSSTANFVVNL
                     GIALFAISFITFFFLKDDTLFYNGVILLFPSRFEQQLSHALSSINRLLRRYFIGLLIE
                     STLIMLLDTLWLVLIGLHFQTAIVIGLIIGILNVIPYVGPLIGIIIGTLIGLASQLQL
                     ISATELLPLTLWMVGAMLATKLIDDAVFQPVIYGNSVKSHPLEIFLVILIAGSLAGVL
                     GMLLAIPAYTVLRVFAKEFFNNFRLVQKITEKI"
     gene            6240..7181
                     /locus_tag="PK502_05940"
     CDS             6240..7181
                     /locus_tag="PK502_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPH00390.1"
                     /translation="MLVNSHLKNGFLLLAGLLVSNAAIAQENLEADSIFTWKLVELTN
                     PSSHSCIQFIPRVDITPVSWDFGDGSPASADVSPVHTYTYGSWSDSVEVTLTYTAGGA
                     NTVKTRQIPLSPAYFHCIHDSNLGQAASFKYIFRNAYMIDNNPGNLGNLHFSWTIDSE
                     PLAENTFSNPDLGQWPNIYYTFLNGGTHQVKLEVYNTTNATEVAGFTHTLTVTPDLSA
                     GVVKLENIPNVFTPNGDGVNDTFTVNTSGTGWFVLRILSRSGGLLYKSESSTLIWDGR
                     NSQGEELPEGIYYYIIEDTTGQYETATGFIYIFRGKK"
     gene            7333..8082
                     /locus_tag="PK502_05945"
     CDS             7333..8082
                     /locus_tag="PK502_05945"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPH00391.1"
                     /translation="MALLEEFEKQGNWLFRYRGVLPLIILAVGAFIYIRAELNPSPSS
                     FMEVNSYKSIYEISCLLVSLFGLAIRVYTIGHTPSKTSGRNIKEQVAESLNTTGIYSV
                     VRHPLYLGNFFMWLGPAMLTANFWFIVSFCLAYWIYYERIMFAEEQFLRRKFGEAYTE
                     WASRVPAFIPCFKHFKKPCHSFCTRKVVAKKKNGLLAVFLIFCSFNVVGELVHKGKHH
                     FNMIFIGMCILSIVLYIVVKIFRKKINLVKQ"
     gene            complement(8127..8765)
                     /locus_tag="PK502_05950"
     CDS             complement(8127..8765)
                     /locus_tag="PK502_05950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPH00392.1"
                     /translation="MAKVDNDMEEYLSRHTSPMADTLMELTRFTYLSTCNPRMISGPI
                     QGKFLQLLCNISRPNRILEVGTFTGYTSICMALGSPPHTVIDTIEVNDELEETIQTYT
                     SMAGVDSKIRLHLGDACQIIPTLDYTYDFAYIDGDKREYPEYYKLVLPKVNSGGLIIA
                     DNVLWGGKVVDASENDEHTKALRAFNTMVQNDMGVENVLLPLRDGIMLIYKL"
     gene            complement(8768..9988)
                     /locus_tag="PK502_05955"
     CDS             complement(8768..9988)
                     /locus_tag="PK502_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPH00393.1"
                     /translation="MEFEQRTLHNGIQIVHKRVSSPVAHCCILINAGTRDELDHEHGI
                     AHFIEHVLFKGTKKRRAFHIMSRLEDVGGELNAYTTKEETVIHASFLKQDFNRAMELI
                     TDIAFHATFPEKEIAKEKEVVIDEINSYKDSPYELIFDDFEELIYPNHPFGRNILGTK
                     NHVSRFNRKGIAAFISRCYNTDQMVFCSVGDIPFTRVSRLADKYLNGISANKRSKPRL
                     ELGNYRPQDKIIKKSTFQTHCIIGTTAYDLQNPKRIAMHLLNNILGGPGMNSRLNLAL
                     REKHGFAYNVESSYTPYTDTGVFTIYFGTDKANVEKSHHEILKELARIREQRMGVMQL
                     FKAKRQLIGQTAIAADSNENLMLSAAKSFMVYQKPEDLTEVFNRIENISSSDILEVAN
                     EILSENRLSTLIYR"
     gene            10260..10332
                     /locus_tag="PK502_05960"
     tRNA            10260..10332
                     /locus_tag="PK502_05960"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10293..10295,aa:Phe,seq:gaa)
     gene            complement(10575..13427)
                     /gene="uvrA"
                     /locus_tag="PK502_05965"
     CDS             complement(10575..13427)
                     /gene="uvrA"
                     /locus_tag="PK502_05965"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPH00394.1"
                     /translation="MEFLKGEEEEVVVEGARVHNLKNIDVAIPRGKLTVITGLSGSGK
                     SSLAFDTIYAEGQRRYMETMSAYARQFLGTLERPDVDKISGLSPVISIEQKTTSHNPR
                     STVGTTTEVYDFLRLLYARASTAYSYNTGEEMVRYTDEQIISLIVEKFTGSRTLLLAP
                     LIRGRKGHYKELFEQLRHKGYLQVRVDDEIIEMRPNMKLDRYKTHHIELVVDKMVVEP
                     DNSKRIAQSVATAMNMGKGTIMVLNADDGSWRYFSRNLMCPTTGISYEEPEPYTFSFN
                     SPKGACPHCNGLGYISEADISRIIPDPSVSIKKGGILPLGPYKNSLIFWQIEAIASKY
                     GFSISDPISTIPEEALNVILYGSEEQFKLKHTGLGLTSSYFLSFEGVVSFVQNHSSND
                     ELNGKGDKWANQFIRQVTCPHCCGSRLKNESLWFKIDGKSIADVASMDIVSLSKWLQG
                     LEDRMNSRQRAIAAEILKEIRERVGFLLDVGLGYLSLSRGTVTLSGGESQRIRLATQI
                     GSKLVNVLYILDEPSIGLHQRDNVRLINSLKQLRDSGNSVIVVEHDEETILSADYVVD
                     IGPLAGRHGGRVMATGTPAEVMKTNSLTAQYLRGEREIAVPTKRRKGNGKIVRIRGAR
                     GNNLKNIDVEFPLGTMICVTGVSGSGKSTLVNETLQPALSQQFYNSYKQPLDFDGIDG
                     IEHIDKVIEVDQSPIGRTPRSNPATYTGVFTDIRKLFELTPDAKIRGFKAGRFSFNVK
                     GGRCETCKGAGVRVIEMNFLPDVYIKCPDCYGQRYNRETLEVKYKGKNISEVLDMTIN
                     QAVEFFGSIPQIHQKLKTLQEVGLGYITLGQSSTTLSGGEAQRVKLSTELAKRDTGKT
                     LYILDEPTTGLHFEDVRILLDVLNKLVDRGNTVIVIEHNMDVVKVADYIIDLGPEGGA
                     EGGKVVCTGTPEELIKSACGYTATYLKRHLNREFNT"
     gene            complement(13449..>14224)
                     /locus_tag="PK502_05970"
     CDS             complement(13449..>14224)
                     /locus_tag="PK502_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPH00395.1"
                     /translation="LDYFDVRESLDRELQVNTFWHSQTVMLLKRANRYFPVIEPILQK
                     NGIPDDFKYLAVAESGLMQVVSPSKAAGIWQILEGTGKDYGLEVNSEVDERYHVEKST
                     EVACRYLNKSYRKYGSWTMAAASYNYGQNGMDKQIDRQQGDSYYNMVLGEETGRYVYR
                     ILAIKVIFENPRKYGFFLDSADLYPPFRYREVSVDTAISSISNFARRFDTNYKVIKLF
                     NPWLRESYLNNKAGKHYRIKIPDINFRVEAYAAGKKDEQ"
BASE COUNT         3564 a         3276 c         3335 g         4049 t
ORIGIN      
        1 tctacccgga atataccgat cagcataact actggggcga tattctctca tgggtcattc
       61 caatcctgct aattgtgggt ctttgggctt tctttttccg tagaatgacc aagaatgcac
      121 ccggaggtgc agccggcccc ggaaacattt tttcggtagg caagtcgaaa gcccaacttt
      181 tcgacaagga atcgaaccaa aagattgatt ttaaggatgt agcaggtctt gaagaggcca
      241 aggttgaggt gatggaaatt gtggattttc tgaaaaatcc aaagaagtac accgagttgg
      301 gcggcaagat acccaaggga gtcctgctgg ttggccctcc tggaaccggt aaaactctcc
      361 ttgccaaagc ggtagccggc gaggctaatg tccccttttt cagcatgtcg ggttccgatt
      421 ttgtggagat gtttgttggg gtgggcgctt caagggtgcg cgacctcttc aagcaggcca
      481 aggaaaaatc accctgtatc atctttattg atgaaattga tgccattggg cgtgcacgtg
      541 gccgaaatgc caacttttcg ggcaacgacg agcgcgagaa taccctcaac cagctgctaa
      601 ccgagatgga tggttttgcc tctaaccagg gtgtaatcat cattgcggcc accaatcgcg
      661 ccgacgtgct ggacaaagct ttactcagag ctggacggtt tgaccgccaa attcacgttg
      721 agctgcccga ctacaaggag cgcctcgata ttttcaaggt acacctccgt cccctcaagc
      781 tagagggcaa cgtggatatc acctttttgg ccaagcagac acccggattt tcaggggcag
      841 atattgccaa cgtatgtaac gaagcggccc tgattgctgc ccgaaaaaac aaaaagcagg
      901 tggagcggca ggatttcctc gatgctatcg accgtataat tggtgggttg gagaaaaaga
      961 ataagattat ttctaaggat gaaaagcgcg ccattgccta ccatgaggct ggtcatgcca
     1021 ccgttagctg gctgctggag tatgccaacc cgctggtcaa ggtttccatc ataccccgtg
     1081 gtatttcgct gggggctgca tggtacttgc ccgaggaacg acaaattact accaccgagc
     1141 agctgctgga tgaaatgtgc gcaacgctgg gcggaagagc tgcagaggag cttgtttttg
     1201 ggaaaatctc taccggagcg ctcaacgacc ttgagcgtgt taccaagcag gcctacgcca
     1261 tgattaccta cttcggtatg ggtgataact tccgtaatat cagctactac gactctaccg
     1321 gtcagtccga attcaccttt agtaaaccct acagcgaaaa aaccgccgag caaattgatg
     1381 aggaggtgaa ggcgataatt gataaagcct acaacagggc aaaggaaata cttagggaga
     1441 atagcgatgg gcacgtgaag cttgccgagc tgcttcttga acgtgaggtg atattcagcg
     1501 aagatttgga aaaaattttt ggcccccgta aaagcctctc ccgcgaggag gagctggcaa
     1561 aagaatttag cgatgagcta aagaaaaccc aatcgccaaa cgagagcgat aatggatgat
     1621 agctggataa aggttttttc aacttctcta ccctggcaag ccgaaatggc caagcaggtg
     1681 cttgaggaga atggaattga agcggttgtt ataaaccagc aggattccag cttttttttt
     1741 ggtgaggcca tcgtttacgt tgagggaaaa tgggaggatc aagcaaaaga actgttaaaa
     1801 ggtattggta gttgactgaa ttcttaaaac gcaccatcag cgggttaatc tttgttgtta
     1861 ctattaccgg agcattggta ataggtcatg ttgctttttt tattgttttc ctcgggctaa
     1921 tggtagctac tcagtacgaa ttctataaaa tgactttcaa aacccatctg cggcctcaga
     1981 tacttcttgg actttttata ggcatcgccc tgttttcatg gtcatttctg tactccctcg
     2041 gaaaggttga acaaattacc atttttggtt tcattccgct actgggcggc atctttgtag
     2101 tggagctcta ccgtaaccag cataaaccaa ttcagaatat tgccgtaacc ctgctgggta
     2161 ttatttatat tgctgtcccg cttagcctgg taaactttat tgtaataaat ggttcctcca
     2221 caagaatttc ctacagccca aacattttgc tgggattact ttttcttatt tggagcaacg
     2281 ataccggagc ctacctgctg ggcgtgagca tgggaaaaca caaactattt cctcgtattt
     2341 cgcctaaaaa aacttgggag ggttttgtcg gcgggctgct cctcaccatg attgtgtcgt
     2401 gggtagatgc aataattttc ccagaggtag cctttaaaca ttggcttgtg attggggtca
     2461 tcacagctat agttggtgct tttggcgatt tggtggaatc aatgttcaag cgaagtgttg
     2521 gagtgaagga ttctggtaag tttcttccaa gccatggcgg atttctcgat aggtttgatg
     2581 cctttctgct ggttattcct tttgtttacg cctatcttga ggtaatgatg ttaatttaat
     2641 tacgcttacc catgaagata caccgggagg gactcgtttt tctggctgta agccttatta
     2701 ttttggcggc gttaaacgta gccgttggcc ttattttacc tgaagttaca tactttttag
     2761 ctcccctttc gttactgata ctgctgtttc tggtcagatt tttcaggatt cccatgcgtg
     2821 gcttttcgcc caacaacgag tatatttact ccccagctga tggaactgta gtggctattg
     2881 aggaggttga agagaatgag taccttaaaa cccgttgcat tcaggtttcg gtattcatgt
     2941 caatttggaa tgttcacatc aactggttcc ccaccactgg caccattaaa tactataagt
     3001 accatcccgg cgactacctg gtggcatggc atccaaaatc atcgtctcac aacgaacgaa
     3061 ctaccgtagt agttgaacgt agcgatggaa ccaaaattct catcaggcag attgcagggg
     3121 cgttagcacg tcggattgtg tgctacgcaa aaccgggcca atcaatagag cagggctcgg
     3181 agttggggtt tatcaagttt ggctcaaggg ttgacctttt cctaccctta aatgcaaatg
     3241 ttgaggtgaa gttgaacgat agagttgtgg gcaagcaaac ggtaattgcc cgactttagc
     3301 aggtttaatc ggcatagctc gagaaaacta tctcccttac ctttcccttg ttgttaaaga
     3361 taaagttgaa cgttctacca tagtactcgt tcgaaatcac cagggtatcg ctgcttgact
     3421 cattaatgtt gctgattgcc ttaagcagct ggtcgcgggt aagcccaacc gagattccgt
     3481 ttaccagctt aatttccgga tttacaattt tcccggccat gagcatttcc cgattaaagt
     3541 aggttttgta aacaaacagc tcacttttct tgtagctaaa cttgtaaatg gtgtctgttc
     3601 tgctaggctc atgagcattg ctacgagcaa atcgggttat ttttgttcgc gctggaagct
     3661 gggtggtgaa tgcttgaata ctttcgtcgt ggccaaatgg tgccttgagg aattcctgct
     3721 tgctaatgcc ctgattggtt gtggttacct tgctcacgct gttacatgag ttgcctgcaa
     3781 cgaggagtaa aactgtgtag cacgctattt ttaatgcggt attcatggtt ggaatttttt
     3841 gctcaaacaa atctacactt ttttgtgaaa tttgtggcaa aattgggtaa tctataaaaa
     3901 gaaagtaacc actccaacaa ccgatacaat tgctcctgca acttctctaa atgttatttt
     3961 ctctttaagg agaaccgccg atggtgcaat aataagcacc gggactatgg acattagggc
     4021 agaggctaca ccggtgctgg tgtagcgaac agctagcagc gagaaagata ctcccagaaa
     4081 tggcccaaag aacgatccaa ccgttatccg acgcatagcc ccggcgtttt ttacggctac
     4141 aagcagattg ccagacttat gcatgaagag cagcactgcg gcaaagccta ctattccggt
     4201 gataaccctt atttgaaccg aggcaactac gtcgtaaccc gccattccat acttgctgag
     4261 aaccagtccg ccagcctggc caactgcacc accaaatgcc agtaacagcc cggggattga
     4321 atacccgaac ttgattttac gaccgataat cttactgccg ttgggggtct tttcgcttct
     4381 aagaactacc attgcaatgc caatgaaaat cagcagcatg cccagggctt gctttagggt
     4441 gagcgtttcg cccattgata gccagcctat tcccgcagca attggcggtg acaacgacat
     4501 gataagcatc gaaatacgag cacctaccag cacgtatgac tgaaacagga atagatcacc
     4561 cataacaaag cccaccaagc ccgaaagcga tagccatagc cactgatgcc ctccggcatc
     4621 agtaggcaat agtaaacctc tggcaaataa tgagtaaagg cttaaaaaaa tgaaggccag
     4681 aaccagcctg ataaaattta ccgataggga gcctactgtt ttgcttgccg attcaaaggc
     4741 caacgaggtt actgtccaaa aaactgcggt tagcaatgcg gccaactcgc ccgtgtgcga
     4801 acttatgaaa tccacttttt ttaaatttgc ttccccgttg gagaacaatt tccctgaata
     4861 gcttaatcgg tcgagaaaat ttatttcgtc atgctaaaaa tcagcccaac aaaccaggtt
     4921 gacctgttgg tatgctaaaa tttattctta tacttgcact atcctttcca gaagatacgg
     4981 ttagctgtaa gcagcatttg tagaaaattt catgcgatga acaagcttgt taagtacatc
     5041 attatcgttt ttgtagttgc agcggtagcc tttcttctct ggtatttctc atctattgta
     5101 gcctacattc tgatctcagc agtgctgagc ttaatgggta aacccattgt agatggggtt
     5161 tgtaggctga aaattaggaa ctggcatcca cccaaggcag taggcgctac ctgcgcgctt
     5221 attgttatct gggtgctatt tgtggcattt tttagcttta tgatacctct ggtggtttcg
     5281 cagtttaact cccttagcgg agttgacgtt ggcacactag tcaataggtt tgcccatcca
     5341 attgccggac tgcaggaatt catccaaaac tacctaccag ctagcgccaa ggagttttcc
     5401 attgaggagt acctgacatc aaaaatcagc acctttttta ctacatccat gattgccaac
     5461 ctgttcagct ctacggcgaa cttcgtcgtt aacctgggca tcgctctttt tgccatatca
     5521 ttcattacct tcttcttcct aaaggacgat accctttttt acaacggggt aatcctgctt
     5581 ttcccgtcaa gatttgaaca gcagctgtcg catgctttat caagcataaa caggctgctc
     5641 cgacgctact ttattgggct cctaatagag agcaccctga tcatgctgct tgataccctc
     5701 tggcttgtac tcattggtct tcattttcaa acggcaatag tgattgggct gattatcggc
     5761 attctaaacg ttattcctta tgttggcccc cttatcggca ttataattgg aacgctaata
     5821 gggttggctt cccagctgca gctgatttcg gctaccgaat tgctgcccct tactctttgg
     5881 atggttggag ccatgcttgc caccaagcta atcgacgatg cggtttttca acctgtgatt
     5941 tatgggaaca gcgtcaaatc tcatcccctc gaaattttcc tggtaattct tattgcgggc
     6001 agccttgccg gcgttctggg aatgcttctg gcaattcctg cctacacggt acttagggtt
     6061 tttgccaagg agttttttaa taacttcagg ctggttcaaa aaattacaga gaaaatttag
     6121 cgtttactga tgtaaaagtg tatttttact gctataaaac aactatatgt ttgtaactta
     6181 ctagttgata aacgcttcac tcactataga tttatcagga aaatttaaca gaaaacctca
     6241 tgctcgtaaa ttctcatcta aaaaacggct ttttgctgct ggcaggtctt ttggtatcga
     6301 atgcagcaat tgcccaggag aaccttgaag ctgactccat ttttacctgg aagctggttg
     6361 agcttaccaa ccccagctcg catagctgca ttcaatttat tccccgagtc gatattactc
     6421 cggttagctg ggacttcggc gatggaagtc cagcctcagc cgacgttagc cctgttcata
     6481 cttacaccta cggcagctgg agcgatagcg ttgaggttac gcttacctat accgcaggcg
     6541 gagccaatac ggttaaaacc cgtcagatac ccttaagccc ggcctacttt cattgtattc
     6601 atgactccaa tctgggacaa gcggcctcct ttaagtatat ttttaggaat gcatacatga
     6661 ttgataacaa cccaggtaac cttggaaatc ttcatttctc atggaccatt gattcagaac
     6721 cccttgccga gaataccttt agcaatccgg acctggggca atggccaaat atttactaca
     6781 cctttttaaa cggaggaacc caccaggtta aactcgaagt ttacaatact actaatgcta
     6841 cagaagttgc cggattcacc cataccctaa ctgtaactcc cgacctttcg gctggcgtag
     6901 ttaagctgga aaatattccc aacgttttta ctcctaacgg tgacggggtg aatgatacgt
     6961 ttacggttaa tactagcgga acgggctggt ttgtgcttag aatcctgtcc cgttcgggtg
     7021 ggctgctcta taaaagtgag tcgagcacac ttatatggga tggccgtaac agccagggag
     7081 aagagttgcc agagggtatt tactactata taattgagga tacaaccggc caatacgaaa
     7141 ctgcaactgg gtttatctac attttcaggg ggaaaaagta acatcctgta aatttaacgt
     7201 atccatagct gttgggctca tgcggatgtt tatgaagcct cgtaacacct cattagggat
     7261 gcaggcaggg ttgaacggca gatttacctg taagcgaaag gtaattaact tgttaactta
     7321 atttagaaaa ctatggcttt acttgaagaa tttgagaaac aggggaactg gctattcagg
     7381 tacaggggcg tgctaccact tataattttg gctgtagggg ctttcatcta catacgagct
     7441 gaacttaacc ctagcccatc atcatttatg gaggttaata gctataagtc gatttacgaa
     7501 atatcctgcc tgctcgtcag cctctttgga ctggccataa gggtttacac aattgggcat
     7561 actccaagca aaacttcagg tcgaaacata aaggaacagg tagccgaatc gctcaacact
     7621 actggcatat actcagtcgt cagacacccg ctctacctcg ggaatttttt catgtggcta
     7681 ggtcctgcca tgctgactgc aaacttttgg tttatcgttt cattctgcct ggcatactgg
     7741 atttactacg aacggattat gtttgccgag gagcaatttc ttaggcgaaa atttggcgaa
     7801 gcctatacgg agtgggctag tagagttcct gcctttattc cctgttttaa gcattttaag
     7861 aagccgtgcc attcattctg cactaggaag gtggtggcta agaaaaaaaa tgggttgctg
     7921 gctgttttcc taattttctg ctcgtttaat gtagttggcg agcttgtgca taagggtaag
     7981 caccacttca acatgatttt tattgggatg tgcatcttgt ctatagtgct ttacatagtt
     8041 gttaaaattt tcaggaagaa gataaatctt gtaaagcagt agtaaagaca atttgctaaa
     8101 gctggttttc cctgcttaca ttccagttat aacttgtaaa tcaacataat accgtcgcgt
     8161 aaggggagca ggacgttttc tacccccata tcattttgaa ccatggtgtt gaacgccctc
     8221 agggctttgg tgtgctcgtc gttttcacta gcatcaacca ctttccctcc ccagagcacg
     8281 ttatctgcaa taattaaccc tccgctgtta accttgggta gaacaagctt gtagtactcg
     8341 gggtactctc ttttatcgcc gtcaatgtag gcaaaatcgt aggtgtagtc aagggtaggg
     8401 ataatctggc aggcatcgcc aaggtgcagc ctgattttgc tatcaacccc ggccatggag
     8461 gtgtaggttt ggatggtctc ctccaactcg tcgtttacct caattgtatc aataactgtg
     8521 tggggtggag agcccagcgc catgcaaatt gaggtgtatc cggtaaaggt accaacctcg
     8581 agtatccggt tgggccgaga tatgttgcaa agcagctgca gaaacttacc ctgaattggc
     8641 ccgctaatca tgcgagggtt acaggtggaa aggtaggtga accgggtcag ctccatcagg
     8701 gtatccgcca ttggcgaggt atggcggctc aggtattcct ccatgtcgtt atcaacctta
     8761 gccatatcta ccggtatatt aacgttgaaa gcctgttttc tgaaagaatt tcgttggcaa
     8821 cctcgagtat gtcggacgat gaaatgtttt ctatgcggtt gaaaacttct gtcaggtctt
     8881 cgggtttttg gtaaaccata aaactttttg cagccgaaag catgaggttt tcgttgctat
     8941 cggcagcaat ggcggtttgc ccaataagct gtctttttgc cttaaaaagc tgcatcaccc
     9001 ccattcgttg ctccctaatt ctggcaagct ccttaagaat ttcatggtgc gatttttcga
     9061 cattggcctt atcggtgcca aagtaaatgg tgaacactcc ggtgtcggtg tatggtgtgt
     9121 atgacgattc cacgttgtag gcaaaaccat gcttctcgcg tagtgccagg ttgagccttg
     9181 agttcatgcc aggccctcca aggatgttat tgagcaggtg catggcaatg cgctttgggt
     9241 tttgcaagtc gtaggcagtt gtaccaataa tgcaatgggt ctgaaatgtt gattttttaa
     9301 tgattttatc ttggggtcta tagttgccga gctctaaacg gggtttactc cttttgttag
     9361 cagaaatgcc gtttaggtac ttgtctgcca acctgctcac acgtgtaaag ggaatatcgc
     9421 ctaccgaaca gaataccatt tggtcggtgt tgtaacagcg gcttatgaac gctgcaatac
     9481 ccttgcggtt aaacctgctt acatggtttt tggtgcccag aatattccta ccaaaggggt
     9541 ggttagggta gataagctcc tcaaagtcat caaatattag ctcgtagggg ctatccttat
     9601 acgagtttat ctcatctatt accacctcct tttccttggc tatctccttt tcgggaaagg
     9661 tggcatgaaa agcgatgtcg gtaatgagct ccatggccct gttgaagtcc tgctttagaa
     9721 agctggcgtg aataaccgtt tcctccttgg tggtgtaagc gttaagttca ccaccaacgt
     9781 cctcaaggcg gctcatgatg tgaaaagccc ttcttttttt ggttccctta aacagaacgt
     9841 gctcaataaa atgggcaata ccatgctcgt ggtccagctc atcgcgagtt cctgcgttta
     9901 tcagaatgca gcagtgggca acaggtgatg aaacccgttt gtggacaatt tgaataccgt
     9961 tatgaagggt tctctgctca aattccatta taccccggta aatttgaatg caaacctact
    10021 ataaataggc aacttgtcaa agtaaaagag ctgccgctaa aatgtggcat acgaaaggta
    10081 tccgttaccc aagcccgtaa tgtaacggct ttggttactg ccatcaaggt tatatcctta
    10141 gcattcagct acatgttggg ttgaattgca tggaagcacc tattaccggt agtaaaatta
    10201 tggaaccaat gtttgcagat acaaaaatat cctatatatt tgcaccgcaa ttgaacgttg
    10261 gtgccatagc tcagttggta gagcaaagga ctgaaaatcc ttgtgtccgt ggttcgattc
    10321 cacgtggcac cacgtaaaac ctccggatat ccggaggttt tttgatttaa aggattttac
    10381 aaactcttgt ttaccctgtt tttttacact ggaagttgcc ggggctaagc cagtgttgtt
    10441 catttatgga ttatccattt acaggttttc gctttaatct gttatggata agtataattt
    10501 gttgcctgga aaatgaaaat gcaggattgg tttaaaaggg tttttatact cctaaggcat
    10561 aggtagaatc ttatttaagt gttaaattcc ctgttgagat gccttttcag gtaggtggcg
    10621 gtgtagccgc atgccgactt aataagctcc tcgggggtac ctgtgcaaac cacctttcca
    10681 ccttctgctc ctccctccgg gccaaggtct ataatgtagt cagctacctt taccacatcc
    10741 atgttgtgct cgatgacaat tacagtgttt cccctgtcaa ccagcttgtt cagcacgtcg
    10801 agtaggatac gcacatcctc gaagtgaagg ccggtagtgg gctcatcgag tatgtagagg
    10861 gttttgccgg tatcccgttt agccagctcg gttgaaagct ttacacgctg agcctcgcca
    10921 cccgaaaggg ttgttgacga ttgccccagc gtaatatatc ccaacccaac ctcctgtagg
    10981 gttttcagct tctggtgaat ctgggggatg ctgccaaaga actccaccgc ctggttgatg
    11041 gtcatatcca gcacctcgct gatgttcttg cccttatact tcacctcaag ggtttcgcgg
    11101 ttgtaacgtt gcccatagca gtcggggcat ttgatgtaaa catcgggcag aaagttcatc
    11161 tcaattaccc taacgccagc acctttgcac gtttcacaac gacctccttt cacgttaaac
    11221 gaaaaccggc ctgccttaaa tccacgaatc ttggcatcgg gtgtcagctc gaacagcttg
    11281 cggatatcgg tgaatactcc ggtgtaggta gccggatttg atcggggtgt tctgcctatg
    11341 ggcgattggt caacctcaat taccttgtcg atatgctcaa ttccatctat accatcgaaa
    11401 tcgagtggtt gcttgtagga gttgtaaaac tgttggctca gcgctggttg aagggtttcg
    11461 ttaaccaggg ttgattttcc gctgcccgaa acgcccgtaa cgcaaatcat ggttccaagc
    11521 gggaactcca catcaatatt tttaaggttg ttacccctgg caccccttat gcggacgatt
    11581 ttcccatttc ccttacggcg ttttgtaggg acagcaatct ccctttcgcc tctgaggtac
    11641 tgggcggtaa gggagttggt tttcattacc tctgcaggtg ttcctgttgc catcaccctg
    11701 cctccatgac gaccggctaa tgggccaata tccaccacgt aatcggccga gaggattgtt
    11761 tcctcatcat gttctaccac tataaccgag tttccggaat cgcgcagctg cttcagggag
    11821 ttaatcaacc ttacgttatc gcgctggtgt agcccaatac ttggctcgtc gagtatgtat
    11881 aggacgttta ccagcttcga cccaatttgg gtggccaggc gaatgcgttg gctttcgcca
    11941 cccgaaaggg taaccgttcc acggctcaac gacaggtatc ctaggccaac atcaagcagg
    12001 aagcctaccc gttcgcggat ttccttcagt atttctgctg caatagctcg ttgccggctg
    12061 ttcatcctgt cctccaaccc ctgtagccac ttcgagaggc ttacgatgtc catggaggca
    12121 acatcggcta tgcttttacc gtctatttta aaccaaagcg actcgttttt aagtcgggag
    12181 ccgcagcaat gcgggcaggt aacctgacgg ataaactggt tcgcccactt gtcacccttt
    12241 ccgtttagct catcgtttga gctgtggttc tgcacaaagc taactacccc ttcaaaactt
    12301 agaaagtatg aagaagtaag ccctaacccc gtatgcttca gcttgaattg ctcctcggaa
    12361 ccataaagga tgacgttgag ggcttcctcg gggatggtag aaattggatc ggaaattgaa
    12421 aacccatact tagaagcaat tgcctctatt tgccagaata tcaacgagtt tttatagggt
    12481 ccaaggggaa gaattccgcc ttttttaatg cttacagaag ggtcgggtat tatgcgggat
    12541 atatcggctt cggaaatgta acccaggccg ttgcagtggg ggcaagcgcc tttcggggag
    12601 ttgaacgaga atgtgtatgg ttcgggttct tcgtaggata ttccggtagt agggcacatc
    12661 aggttgcggc tgaagtatcg ccacgaacca tcatcggcgt tgagcaccat gattgtccct
    12721 ttgcccatgt tcatggctgt ggccaccgat tgagcaatac gcttggagtt gtccggttca
    12781 accaccatct tgtccaccac cagctctatg tggtgggttt tatagcggtc gagcttcatg
    12841 tttggccgca tctcgataat ctcatcgtca acccttacct gcaggtagcc cttgtgccga
    12901 agctgctcga atagctcctt gtagtggcct tttcgtcctc gaatgagtgg agcaagcaaa
    12961 agggttcggc tgccggtaaa tttttcaaca attaggctga taatttgctc gtcggtgtag
    13021 cgaaccatct cctcgccggt attgtaggag taggcggttg aagcgcgagc gtagagcaac
    13081 cgtaggaagt cataaacctc ggtagtcgtg cccaccgtgg aacgtgggtt gtggctggtg
    13141 gttttttgct cgatggaaat tacggggcta aggccggaaa ttttatccac gtcgggacgt
    13201 tcgagcgttc ccagaaactg acgtgcataa gccgacatgg tttccatgta ccttcgttgc
    13261 ccctcggcgt atatggtatc gaaggccagc gatgactttc cgctaccgct caaaccggta
    13321 ataaccgtta gttttccacg aggtatggca acatcaatat ttttcaggtt atgcacccgg
    13381 gcgccctcaa ccacaacttc ttcttcttca cccttcaaaa attccatttc taactccaaa
    13441 ttagtcccct attgctcgtc cttcttccct gctgcgtaag cttcaaccct gaaatttata
    13501 tccggaattt taattctgta gtgtttccct gccttgttgt tcaggtagct ttcgcgtagc
    13561 catgggttga aaagtttaat taccttgtag ttggtatcaa accggcgggc gaaattagaa
    13621 atgctggaga ttgccgtatc aacgctaacc tctctatacc taaacggggg gtaaaggtca
    13681 gccgaatcaa ggaaaaaacc atacttacgg ggattttcaa aaattacctt gatggccaaa
    13741 attctataaa catagcgacc ggtttcctca cccagcacca tgttgtagta tgaatcgccc
    13801 tgctgtcggt caatttgttt atccatacca ttttggccgt agttgtaaga ggcagcagcc
    13861 atggtccagc tgccgtattt tcggtagctt ttgttcaaat accggcaggc cacctcggtt
    13921 gatttttcaa cgtggtagcg ctcgtcaacc tcactgttta cctcaagacc gtaatctttg
    13981 cccgtgccct cgagaatttg ccaaattccg gcagcctttg atggggaaac aacctgcatg
    14041 agaccgcttt cggcaacggc aaggtattta aaatcatcgg ggattccatt tttttggaga
    14101 ataggctcga taaccgggaa gtaccggttg gctcttttga gcaacataac ggtttgggag
    14161 tgccaaaagg tatttacctg cagctccctg tccaggcttt cgcgcacatc gaagtaatcg
    14221 agtg
//
LOCUS       DAOPHX010000061        14171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_4982_length_14171_cov_5.051218, whole genome shotgun sequence.
ACCESSION   DAOPHX010000061 DAOPHX010000000
VERSION     DAOPHX010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 14171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14171
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4982_length_14171_cov_5.051218"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..399)
                     /locus_tag="PK502_05975"
     CDS             complement(175..399)
                     /locus_tag="PK502_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HPH00396.1"
                     /translation="MKTEQFSVGKMKCMGCVATITSGLSEIEGVSKVNAELESATVTV
                     TYENDGLREVIINKLEALGYPVNLDIKQGS"
     gene            complement(472..630)
                     /locus_tag="PK502_05980"
     CDS             complement(472..630)
                     /locus_tag="PK502_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HPH00397.1"
                     /translation="MVQEIVVYMLIIAAILYVGYSIYRVFRPKQKNLGHCAGCSSSSC
                     GIRELKSK"
     gene            complement(632..3136)
                     /gene="feoB"
                     /locus_tag="PK502_05985"
     CDS             complement(632..3136)
                     /gene="feoB"
                     /locus_tag="PK502_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPH00398.1"
                     /translation="MQLSELHNGEYGIISKIRGRGAFRKRITEMGFVKGKRVDVVKNA
                     PLNDPIEYSIMGYQVSLRRSEASLIEVVTPDEAKKILAENHTEPLTTLTEPELVQTGL
                     KVMGKTINIALVGNPNSGKTTIFNFLSGSREHVGNYSGVTVDSKIAHFQHKGYTFNIT
                     DLPGTYSLTAYTPEEVYVRNYIHENHPDLIINVVDASNLERNLYLTTQLIDMDVRIVV
                     ALNMYDELLDKRDRFDYEMLGKMLGIPFVPTVGSRAKGFDRLKDTIINVFSEKDKTSR
                     HIHINYGAVIEESIAAIQHEVRKNETLTVNYSSRYLSIKLLEKDRLTDRMLKDFPNYT
                     EIRKIARQEVERLEALFHEDSETLITDAKYGFIAGALKETYHESSIKKRQTTEIIDTF
                     ITHKLFGFPIFIFLIWLTFFTTFTLGEYPMNWIEAGVAWISRSINGLMPNGPLKDMLV
                     DGIISGVGSVIVFLPNILILFFFISLLEDTGYMARAAFIMDKLMHKIGLHGKSFIPLV
                     MGFGCNVPAIMASRTIENKNNRLVTMLITPFMSCSARLPVYILILGTFFPKHAGNLLV
                     LIYFTGILLAVGTALLLKKTLFRTDDVPFVMELPPYRIPTLRSILIHMWDKASQYLNK
                     IGGVILVGVIIIWALGYFPRNVQTSKDYDSLIGLKRAALTSAQATNAATAESLEQEIQ
                     EIEMEREALRQEQSYLGRIGKFIEPALKPLGFDWKMGVSLISGIAAKEIVVSTLGVLH
                     LAKEPNGSQSLPDKLKADTYTTGEKVGQKVFTPLSSFSFLIFILIYFPCVAVISAIKQ
                     ESGSWKWALFTIVFTTALAWVSSFLFFQVGTLITGS"
     gene            3346..4116
                     /locus_tag="PK502_05990"
     CDS             3346..4116
                     /locus_tag="PK502_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00399.1"
                     /translation="MEANEPEEIFRQIQEFLGNAGKRLSIVEDVVDISIQKDYFRMAE
                     QFRNKQDTKEVNVSADILFSNDTTEEQLKEVLVLLASSGEVESYRAIEKFVSVAPSQL
                     KGWALLALNECRFHLVGALTDEEGVLISTGMGGLDGKLRYFIVLIPAVDMLSQQQAKL
                     VTDEVKFIFKHYDSEVERVEMADRYMCITALVPMSVPVNQPVTAVIKECNGMGNLLDE
                     GYIVTNVKTLAPDEIRKVLNREPIDGIYFDDNPDHTYK"
     gene            complement(4308..4688)
                     /locus_tag="PK502_05995"
     CDS             complement(4308..4688)
                     /locus_tag="PK502_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HPH00400.1"
                     /translation="MTDQLDNQNEFDRAEIEDLFSNVVKAGKRTYFFDVKATRSDEYY
                     LTITESKKRMGKDGNMFYEKHKIFLYKEDFDNFMGGLTEAVNFIKSNQEVLPRIAAEY
                     GNEASLAEVNDELTTLNFEDLDTK"
     gene            4912..5907
                     /gene="nusB"
                     /locus_tag="PK502_06000"
     CDS             4912..5907
                     /gene="nusB"
                     /locus_tag="PK502_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPH00401.1"
                     /translation="MINRRLLRIKILQVLFAYFRSNNDSLSNLEKELHFSIEKSYQLY
                     LLVLLLPEALADVALEKIELGKQKFRPTADELNPNTRFVDNPLIHILRNNQALKQKAK
                     DSKLSWKNYPEVVKLLYQQLVSSESYHKFMSDDEFSLDTCRQMIVNFYLNELENSDEF
                     DAFLEEQSIYWNDDLKFVLSMAAKTIKKVREENPDALKLMPLYRNEEDKDFAFQLMTK
                     AVLKHSDNVGLIEEYTQNWEVDRIASMDMLIMEMAITEVLEFPSIPVKVSLNEYIEIA
                     KFYSTEQSGLFINGVLDKIITTLRQQKKFEKQGRGLIGDIAETPDDINSLEPDTE"
     gene            6029..6448
                     /locus_tag="PK502_06005"
     CDS             6029..6448
                     /locus_tag="PK502_06005"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPH00402.1"
                     /translation="MRTRGMVVMCVLLLTTCGSPSKPKNNLSTSGNSSGVPNLEYIED
                     FFDFGTITQGEVVTHTFRFKNSGNAPLVVKDVIPGCGCTTSKLSSTILKPGEEGTVEV
                     IFNSQGWAGLQYKTITLRTNSSIMDKSVTIRANVVMP"
     gene            6515..6832
                     /gene="yajC"
                     /locus_tag="PK502_06010"
     CDS             6515..6832
                     /gene="yajC"
                     /locus_tag="PK502_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPH00403.1"
                     /translation="METLFVLLQKPAAQGANPLMTILMFALIIVVFYFFMIRPQMKRQ
                     KELRKFQEALKKGDKVIIAGGVYGKISEVKDDYVMVEISDNINVKVAKSTIIRDVSDI
                     QAK"
     gene            6902..7900
                     /locus_tag="PK502_06015"
     CDS             6902..7900
                     /locus_tag="PK502_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00404.1"
                     /translation="MDSTLLKQFNKIIYREKANFKKRLAVFSLFLVISAILWFLLKLS
                     HEYSDELVYPVRYSNLQKGKIITGTPPEKISVKVKAYGYTLLRYKFTSYSYPLNIDLK
                     ELKPNQGKNSSSQFCVSTTQQFSSISAQLGKDMDVLDVFPDSICLELEDVVEKNVRVV
                     SNLSLTFARQYMLAGNIEVEPGEITISGPAAILDTINELKTQPVSLNLLAGNKKIILN
                     IPQVKQVFMPNSEVEVFIPVEKFTEVMLNVPIIPLNVPPSYDAILLPAEIQLRCNVTL
                     SKYFKLKPSNFRAVCNISDTGSMEGNKLKVLLENHPDFVSRVSFEPLYVDYILKKK"
     gene            7897..8484
                     /gene="coaE"
                     /locus_tag="PK502_06020"
     CDS             7897..8484
                     /gene="coaE"
                     /locus_tag="PK502_06020"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPH00405.1"
                     /translation="MSLMVGVTGGIGSGKSLVCSILARLGYPVYNSDIASRTITDSDE
                     GVKQRVKLLFGEEIYCNGKLNRQRLASMVFADNMLLQQLNSIIHPVVMDDFEKWSETH
                     KDNQVAFMESAILFESGIFKKLDKTVLVVAPADVRIRRVMARDKVNKEAVQARVSNQM
                     DDELKVKLADFIINNDEKSLLLPQVIEIVERLKPL"
     gene            8489..9208
                     /locus_tag="PK502_06025"
     CDS             8489..9208
                     /locus_tag="PK502_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HPH00406.1"
                     /translation="MGKYGKWISGGLGWALFGPIGAILGFAIGSIFDSAQTPTKIYTG
                     ETSRNGFLVSLLVLVSAVMKADGRVLKSELSYVKDYFYKNFGASASREAILLLRDILK
                     QSVPVKDVCKQVKENVDYHSRLQMLHFLFGIAQADGVVSPAELQIINEIAQYLGVETA
                     DFDSIKAMFIPDTDKYYKILEVSPSAANDEIKKAYRQMAIKYHPDKVSHLGEDVRQAA
                     EQKFKMVNEAYEKIKKERGFN"
     gene            9218..9508
                     /locus_tag="PK502_06030"
     CDS             9218..9508
                     /locus_tag="PK502_06030"
                     /inference="COORDINATES: protein motif:HMM:NF016109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop repeat-containing protein"
                     /protein_id="HPH00407.1"
                     /translation="MNEAVEAIGWFGNIILSIGVIPQVVQTWRSHNVSSFNWVFLLLW
                     ALGVFFTFIYIAYNDMVSGKYQWPLWLNYLVNIAATFYLVWAKFHYGRKSNR"
     gene            9624..10091
                     /locus_tag="PK502_06035"
     CDS             9624..10091
                     /locus_tag="PK502_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HPH00408.1"
                     /translation="METTLKEIISISGYSGLFKIVSQGRKGVIVESIADGNRMTAPIT
                     SKVTALTDISVFTNSTDLPLIEVLKLIRNFEESKPTLNPKTSTDKAIREYFEKVLPDF
                     DRERVYTSDIKKILSWYNQLQANNLLAILDVEEAKPEEPAAEEHSNTAQDAES"
     gene            complement(10160..12154)
                     /locus_tag="PK502_06040"
     CDS             complement(10160..12154)
                     /locus_tag="PK502_06040"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPH00409.1"
                     /translation="MKRCLVVSLIVLLAASAKGQNKEEDIFNLSLEELLNLEVSISTK
                     TATNIRNTPGILTVITSEDIKNSGVNGIIDLFQLFVPGFDFGVDVEGVVGMGVRGIWA
                     HEGKVLFMVNGLELNDGMFASVPFGNHFPLNNIERIEVIRGPGSAMYGGFAGMGVVNI
                     ITRDVYKPGGYVSYTANHTGRQFSQNNVGFGTGVHQNYLNIGISGNYGNGSRSDRDYV
                     DYHGQSRSMRNASDTYTRQLDLKLNYKNFNLVGFIDDYSYQQIDLWDSYYPGPPLTES
                     FKSSLVQATYDFKPNGNLTITPKISYKWQQPWKLTALGAGYGYNNNKTFDGLLGGIST
                     SYTKNKLTIVGGAELKHDHLNQPELVDTVYEETFRNGKEQLSYTNTAIYGLLQHDSRV
                     GNFTLGARYDFSSEYGSSFVPRVGIAKVFDFMHFKVMVSQSFRVPGGILPNRLPQGNP
                     EIKPEKGTSYEVAIGFKMPFQSYITFNIFDVSFDKVIVYNLGNDDITGVGYYKNEGTI
                     GTRGIEADFRHISTKISSFATFAYYMRKHSKTDSLYRVPDRESYFLGFSPLRLNGGFN
                     FRVNNRLSLTLSGSYFGKRYAFTQREGNGMDILEKLNPLFMLGCNVNIKNIVLKGLTA
                     TIEVNNMLGESFVFPQPYKGSHAPLPGLDRSFGFNLVYEY"
     gene            complement(12356..13024)
                     /locus_tag="PK502_06045"
     CDS             complement(12356..13024)
                     /locus_tag="PK502_06045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPH00410.1"
                     /translation="MKTFSKAIDAAIFDMDGVLVNNHQYHLNAWKEFCRRHGYKFDEK
                     TFTTAYFGKNNQEIISELTGKSISTHEAELLGEEKEAIYRDIYLPFIKPVDGLIELLD
                     YLKENGVRLAVATSAPKSNFKFIEKSLRLENRFDVVVDASMVQHGKPDPEIYLKASEL
                     LKVHPEGCLVFEDSFSGIQAATAAGMRVVAMATTHTRNELGKNLTVADSFYDVLAFIL
                     RENE"
     gene            13246..13692
                     /locus_tag="PK502_06050"
     CDS             13246..13692
                     /locus_tag="PK502_06050"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPH00411.1"
                     /translation="MKSLGIVFIMILSPLVMLGQHVKMDKDGIKHGPIIGAIPDTVKL
                     GEIFLDELDDGNGKVQLTVVNQGNKPLILTRVSGCCGTNIKSWPKAPILPNQKGTIEV
                     YFRIENRPQRISRTVTIESNAANSPVVKVPIIGVVVERKAKNELTL"
     gene            13753..>14171
                     /locus_tag="PK502_06055"
     CDS             13753..>14171
                     /locus_tag="PK502_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00412.1"
                     /translation="MKERLHSTTFDLPVQELRRGYRSDVYFWREKVVLESHNLHPHVT
                     MQVFQKNEAVVCGIDEAVAVLKVASGRYSDYDQAYKLFDKLIELKRMGRKVFLTDKKK
                     YLQLEEEKMKVSQALDKLWVDGFSDLKIDALWDGDAIS"
BASE COUNT         4057 a         2925 c         3006 g         4183 t
ORIGIN      
        1 gaagtaaaat tatcaacatg ggtgttctaa ccactaagtt ctcatgcata aaagcatgga
       61 ctaaagtatt tgtaatcgga aacctaggtg tttttgtaat taagggatac caaaagagga
      121 agtgatacct tcacttcctc tacctgaata catgaatgtg cagatggttt tcggttagga
      181 gccttgctta atatcaaggt taaccggata cccaagggct tcgagcttat tgattattac
      241 ctctcgtaaa ccatcattct cataggttac cgttacagtt gccgattcaa gttcggcatt
      301 tacttttgat actccttcaa tttcagaaag accgctggtg attgtagcta cacatcccat
      361 acacttcatc tttcccactg aaaattgttc tgttttcatg gttgctataa atttagtttt
      421 aagtttcaca taatttgtaa tgccattcgt ttgacactca attagtagag cttacttact
      481 cttcaactcc ctaattccac aagatgagga gctgcatccg gcacaatggc ccaaattctt
      541 ttgctttggc ctgaataccc ggtaaatgct gtatcctaca tagaggattg ctgctattat
      601 caacatatat actacaattt cctgaaccat actagcttcc tgtaattaaa gtcccaacct
      661 gaaagaatag aaacgatgaa acccacgcca gcgcagtggt gaaaacaatg gtaaaaagag
      721 cccatttcca cgatcctgat tcttgtttaa tagccgatat tactgctaca caggggaagt
      781 aaattagaat aaatattaaa aaggagaatg aactaagggg cgtgaatacc ttttggccaa
      841 ccttttcccc agtagtgtaa gtgtcggcct taagcttgtc gggtagcgat tgtgaaccat
      901 ttggctcttt ggccagatga agtaccccca gggtgcttac cacaatttcc ttggcagcaa
      961 tccccgaaat caggctaact cccattttcc aatcgaatcc aagcggtttg agggcaggtt
     1021 cgatgaactt tccaattctt ccaaggtacg actgttcttg tcgcagggct tccctttcca
     1081 tctcgatttc ttggatttct tgctccaggc tctctgctgt ggcagcgttg gtggcttgtg
     1141 ctgaagttag agcagcacgt ttaagcccaa tcaaactgtc ataatctttt gatgtctgta
     1201 catttcgtgg aaagtatcct aaagcccata taatgataac ccctaccaga attactcctc
     1261 cgattttatt aaggtactgg gaggctttat cccacatgtg aataagtatc gaacgtaggg
     1321 ttggaattcg gtagggtggc aactccatga caaagggaac atcgtccgtg cggaatagcg
     1381 ttttttttag cagcaatgcc gttccaacgg cgaggagtat gccggtaaag tatatcagca
     1441 ccaacaggtt gccggcatgt tttgggaaaa aggttccaag aatcagtata taaacgggaa
     1501 gccgggcgct acagctcatg aatggagtta taagcatggt aaccagccgg ttattcttat
     1561 tttcaatggt tcgtgaggcc attatagccg gcacgttaca tccaaacccc ataaccagcg
     1621 ggatgaacga tttgccatgc agccctatct tgtgcatcaa cttatccatg atgaaggcag
     1681 ccctggccat gtacccggta tcctccagaa gggatataaa gaagaaaaga atgaggatat
     1741 ttggaaggaa gactattaca ctaccaacac cgctaataat tccatctact aacatgtcct
     1801 ttaatgggcc attgggcatt aggccattta tagaacgact aatccatgca acccccgctt
     1861 ctatccagtt catggggtac tcacctaaag taaaggtggt gaaaaaggtg agccagatta
     1921 aaaaaatgaa aattgggaac ccgaagagct tatgggtgat aaacgtgtca ataatttcag
     1981 ttgtttgcct tttttttatg ctgctttcgt ggtaggtttc ctttagggct cctgcaatga
     2041 atccgtattt ggcatcagta atcagggttt cagaatcctc atggaacagc gcttctagcc
     2101 tttctacctc ttgccgggca attttgcgta tttcagtgta atttggaaaa tccttcagca
     2161 tgcggtcagt cagcctatct ttttccaaaa gctttattga taggtacctg ctggagtagt
     2221 taaccgtaag agtctcattt tttctaacct catgctggat tgctgctatt gattcctcaa
     2281 ttacagctcc atagtttatg tggatatgac gcgaggtttt atccttttcc gagaatacgt
     2341 ttataatggt gtccttcagc ctgtcgaaac ctttagccct ggagccaacg gtgggaacaa
     2401 acggaatgcc tagcatttta cccagcattt cgtagtcaaa cctgtccctt ttatccagca
     2461 gctcatcata catgtttagg gcaaccacaa tcctcacatc catgtcgatg agctgggtgg
     2521 tgaggtaaag gtttcgctcg aggttcgagg catcaactac gttgataata aggtcgggat
     2581 ggttttcgtg gatgtagttt ctaacgtaaa cctcctcagg ggtgtaagct gttagcgagt
     2641 aggttcccgg taggtcggta atgttgaagg tgtacccctt gtgctgaaaa tgggctattt
     2701 tggaatcaac cgtaacgccg ctgtagtttc ccacgtgctc tcttgaaccg gaaaggaagt
     2761 tgaatatggt ggttttaccg gaattgggat tcccaacaag ggctatattg atggtttttc
     2821 ccatcacctt caagccggtt tgcaccagtt ccggttctgt tagggtagtt aaaggctcgg
     2881 tgtggttttc ggccaatatt tttttcgctt catcgggggt tacaacttct atgagggaag
     2941 cctcgcttct gcgaagcgaa acctggtagc ccataatgga gtactcaatg gggtcattca
     3001 atggggcatt cttcaccaca tccacccttt tacctttcac aaaacccatc tcggtgatac
     3061 gcttcctaaa cgctccgcgt ccccttattt tggaaataat cccgtactcc ccgttatgaa
     3121 gctccgatag ctgcattttt ttgttataat attctagtaa ctttgatttc gcataattgt
     3181 ttaaatggag cacaaatata acattcagcc aaaactttaa aagccgcaac gtgagtaatg
     3241 ctatttatgg caacattcac attgtcaact cccttaaagc tcagggcacg atgtgcgctg
     3301 ttaaaaataa gctgctttac aaacgttaaa taattttatt ctggtatgga agcaaatgaa
     3361 cccgaagaga tttttaggca gattcaggag tttttaggca atgccggtaa acgattgagc
     3421 atagttgagg atgtggttga tatttccatt caaaaggatt acttcagaat ggcggaacaa
     3481 ttccgtaata aacaagacac taaggaggta aatgtttctg ccgatatttt attctcgaac
     3541 gatacaacgg aagagcagct aaaggaagtt ttggtgcttt tagcctcatc aggggaggta
     3601 gaaagctaca gggcaataga aaagtttgtt tctgttgccc cgtcccagct aaagggatgg
     3661 gccttgctgg ccttaaacga gtgcaggttt catctggtag gtgccctgac tgacgaggaa
     3721 ggggtgctta ttagcacagg aatgggagga cttgatggga aacttcgcta ttttattgtg
     3781 cttattcctg ctgttgacat gctaagccag cagcaggcta aactggttac ggatgaggtt
     3841 aagtttattt tcaagcacta cgattctgaa gtggagcgtg ttgaaatggc cgatcggtac
     3901 atgtgcatta ctgctctagt acccatgagt gttcctgtaa atcaacctgt tacggcggta
     3961 attaaggagt gtaacggcat gggcaacctg ctcgacgagg gctatatagt taccaatgtt
     4021 aagactttgg cgcctgatga aatacgtaaa gtgctcaaca gggagcccat agacggaatt
     4081 tattttgacg acaaccctga tcatacctac aagtaaattc aaaagcacca ccaatattgt
     4141 tattagtttc cttgtaaaaa ttcaagttgg ggaaccggca gcttacagat gcggaacttt
     4201 ttgatctgta tatataaccg gattcaccta ctaaacttaa atttatatcg gaaaactata
     4261 acaaaaaagc cggcaaatag ccggcttgca tgctctagtt taattatcta ctttgtatct
     4321 aagtcctcaa agttcaacgt agtaagctca tcgtttacct cggccagtga agcttcgttg
     4381 ccatactctg ccgctatgcg gggaagcact tcctggttcg acttgatgaa gttgactgct
     4441 tcagtcaaac cacccataaa gttatcaaaa tcttctttgt acaggaatat tttatgcttt
     4501 tcgtagaaca tattgccatc cttgcccatc cttttcttgc tttcggtaat ggtaaggtag
     4561 tactcatcgc tacgggtcgc ttttacatca aaaaagtagg tacgcttgcc agcttttacc
     4621 acatttgaaa aaaggtcttc aatttctgcc ctgtcaaatt cattttgatt atcaagttga
     4681 tcggtcatca ctgtaagagt tttagtttaa ggataaacaa tgcttttatg accatcaaaa
     4741 ataaaaaatt aatcgtctat ccaacctttt gattttaaaa aattatttac cagaatgttt
     4801 tggttttgtt ttatatttta ttaaccgcaa gattatttta tatctaatat agctactcat
     4861 gctattttgt tttaactttg taggcttttt actttgaaaa tttgttctta aatgattaac
     4921 cgacgcttac tccggataaa aattctacaa gttctttttg cgtatttccg aagcaataac
     4981 gattcgctta gcaaccttga aaaggagctc cattttagca ttgaaaagtc ctatcaactt
     5041 taccttttag tgctattact tcctgaagca ctcgccgatg ttgccctgga gaaaattgag
     5101 ctgggtaaac aaaagttccg tcctaccgcc gatgagctaa atccaaatac ccgttttgtt
     5161 gacaatccgc taattcatat tctcagaaat aatcaagcgc tcaagcagaa agcaaaggat
     5221 tccaagttga gctggaaaaa ctaccccgag gtggttaaac ttctctacca gcagctggtg
     5281 tcctccgaaa gctaccataa attcatgtcg gacgacgaat tttccctcga tacctgcagg
     5341 cagatgattg ttaacttcta cctaaacgag cttgaaaatt ctgatgaatt tgatgctttt
     5401 ctcgaggagc agagcatcta ctggaacgat gacctgaagt ttgtgcttag catggctgct
     5461 aagaccatta aaaaggtaag ggaagaaaat cctgatgctc taaagttgat gcctttatac
     5521 cgaaacgagg aggataagga ctttgctttt cagctaatga caaaagcggt actcaagcat
     5581 tctgataatg ttggcctcat tgaggagtac acccagaact gggaggttga ccggattgcg
     5641 tcaatggata tgctgatcat ggaaatggct attaccgagg ttcttgaatt tccatcaatt
     5701 ccggttaaag taagcctaaa tgagtacatc gaaattgcaa agttctacag cacggagcaa
     5761 agcggacttt ttataaacgg tgtactcgat aaaataatta ccacactccg ccagcaaaag
     5821 aaattcgaga agcaggggag aggacttatt ggcgatatag ccgaaactcc tgatgacata
     5881 aacagcttag aaccagatac cgaatagcca ggctaaattc agcaaccacc aacatccgca
     5941 ccctgaagta gcaatttcag ctgttttcag ttgcaaattc aaaaggttta tagaacaagt
     6001 tcagctaaaa attactaaat attctggtat gagaacaaga ggaatggttg ttatgtgtgt
     6061 actgctgctt accacctgtg gctcgccctc caaacctaaa aataatctga gcacctctgg
     6121 taacagctca ggggttccaa acctggagta tatcgaagat ttttttgatt tcggcacaat
     6181 tactcaggga gaagtggtaa cccacacatt ccgttttaag aattccggaa atgccccgtt
     6241 ggtggtgaag gatgtaattc ctggttgcgg atgcactacc tctaagctaa gcagcaccat
     6301 tttgaaaccc ggtgaggagg gaaccgttga agttattttc aacagccagg gatgggccgg
     6361 attgcagtat aaaacgatta cccttcggac aaattcatcc attatggaca aatcggttac
     6421 tatcagagca aacgtcgtga tgccataggt tgcttcttaa aaaaatacta cctttgcttc
     6481 gaaatttaat gcttaacaaa ctaaacactt aaagatggaa acactattcg tactattgca
     6541 aaaaccagct gcacaagggg ctaacccctt aatgactatt ttaatgtttg cactgatcat
     6601 cgtggtattt tactttttta tgatccgtcc gcaaatgaag cgccaaaaag aacttcgcaa
     6661 gtttcaggaa gccctgaaaa agggggataa ggtgattatt gccggaggtg tttacggtaa
     6721 aatcagcgag gttaaggatg attacgttat ggttgaaatt tctgataaca tcaacgttaa
     6781 ggttgctaaa agcacaataa ttcgtgatgt ttccgatatt caagccaaat agctataacc
     6841 ttgatgaaag cattgcagga ttcagccttg ggcaacgaat cctgcattta cttccttaaa
     6901 gttggattca accctactca aacagttcaa caagataata tatcgcgaaa aggctaattt
     6961 taaaaagcgc ttggcggtat tttccctttt tttggttatt tctgccatac tctggttcct
     7021 tctaaagctt agccacgagt actccgatga gctggtatat cctgtaaggt attcaaacct
     7081 ccagaaggga aaaattatta caggcacccc ccccgagaaa attagcgtaa aggttaaagc
     7141 atacggctat accctcctta ggtataagtt tacaagctac agctaccccc ttaatattga
     7201 tttaaaagaa ttaaaaccta accaaggcaa aaattcatcc agccaatttt gtgtttcaac
     7261 tacacaacaa ttttcttcca tttcagcgca acttggtaag gatatggacg tgctggacgt
     7321 tttccccgat tccatttgcc ttgaacttga ggatgttgtg gagaaaaatg ttagggttgt
     7381 ttccaacctt agcctgactt ttgcacgaca gtacatgctt gccggaaaca ttgaggttga
     7441 acctggtgaa atcactattt ccgggcctgc tgctattctc gataccatca acgagctcaa
     7501 aacccaaccc gtaagcctaa atctccttgc tggaaacaag aaaattatat tgaacattcc
     7561 ccaggtaaaa caagttttca tgccaaattc ggaggttgag gtatttatac ccgttgaaaa
     7621 gtttacggag gttatgctta atgttccgat tatccccctt aacgtacctc ccagctacga
     7681 tgcaatttta ttaccggccg aaattcagct taggtgtaac gtaaccttga gcaagtactt
     7741 caagttaaaa ccttcaaatt tcagggcggt atgcaacata tccgacaccg gttcaatgga
     7801 gggcaacaag ctaaaggttt tactcgaaaa ccaccccgat tttgtcagtc gggttagctt
     7861 tgaacccttg tatgttgact atattctgaa gaagaaatga gcctgatggt tggggtaacc
     7921 ggaggaatcg gaagcggaaa atcgctggtt tgcagcatac ttgccagatt gggatatcct
     7981 gtctataatt ctgatatagc ctccaggaca attactgata gcgatgaagg agtgaagcag
     8041 cgcgtgaagc tgctttttgg agaggaaatt tactgcaacg gcaagctcaa ccggcagcgg
     8101 cttgcatcaa tggtttttgc ggataacatg ctattgcagc agctcaatag cattatacat
     8161 ccggtggtaa tggacgattt tgaaaaatgg tcggagaccc acaaggataa ccaggttgct
     8221 ttcatggagt cggccatcct ctttgaatct ggtattttca aaaaacttga taaaacggtg
     8281 ctggtcgtgg cacctgctga tgtaagaatc aggagggtta tggctcgcga taaggtaaac
     8341 aaggaagcag ttcaagcacg agtgagcaac caaatggatg atgaattgaa agttaagctg
     8401 gctgacttta tcattaataa tgatgaaaaa tctctacttt taccccaggt tattgaaata
     8461 gtcgaaaggc ttaaacctct ttaaacaaat gggtaaatac ggtaaatgga ttagcggcgg
     8521 tttaggctgg gctttatttg ggccaattgg ggccatcctg ggatttgcta ttggctctat
     8581 attcgacagc gcccaaaccc ctactaaaat ctatacgggc gaaaccagcc gaaatggatt
     8641 cctggttagc ttgcttgtgc tggtttcggc tgtgatgaag gccgatggca gggtgttaaa
     8701 atcggagcta agctacgtaa aggactactt ttacaaaaat tttggagcct cggcttcccg
     8761 ggaggctatt cttttgctga gggatatact taagcaatcg gttcctgtga aggatgtgtg
     8821 caagcaggtt aaggagaatg tagattacca ttcccggttg caaatgctgc acttcctttt
     8881 cggaatagct caggctgatg gagttgtaag cccggctgaa ctccaaatta ttaatgaaat
     8941 tgctcaatac ctcggagttg aaacagctga cttcgactcc ataaaagcca tgtttattcc
     9001 cgataccgat aagtactaca aaattttgga ggtttcaccc tctgctgcta acgatgagat
     9061 aaaaaaggcc taccgacaaa tggccataaa ataccacccc gacaaggtaa gccatttggg
     9121 agaggatgta cggcaagctg ccgaacaaaa atttaaaatg gtgaacgaag cctacgagaa
     9181 gattaaaaag gaacggggtt ttaattaaca acacaatatg aacgaagcag ttgaggctat
     9241 cggatggttt ggcaatatta ttctgagcat tggtgttatc cctcaggttg ttcaaacgtg
     9301 gcgatcgcat aacgtgtcga gttttaactg ggtttttctt ctgttatggg ctcttggtgt
     9361 tttttttacc tttatttaca ttgcttataa cgatatggta agcggaaaat accagtggcc
     9421 attgtggtta aactacctgg ttaacattgc tgcaaccttt tacctggtgt gggcaaaatt
     9481 ccattatggc cggaaaagca accggtaaac tttcagctac ccagacgccg gctatttttg
     9541 tgtattgttc atttaaacgg tacgcagttt ttaatttaaa attttttcaa cttaacttta
     9601 caccgaaaat ataactagga agtatggaaa ctacacttaa ggaaatcatt tctatttcgg
     9661 gctactccgg tttattcaaa attgtatcgc aagggcggaa aggggttatt gttgaatcca
     9721 ttgctgatgg aaaccgaatg acggccccca ttacctcaaa ggttaccgcc ttaaccgaca
     9781 tatcggtttt tactaattcc accgatttac cccttatcga ggttcttaag ctaatacgta
     9841 acttcgaaga atctaaaccc actttaaatc ctaaaacttc gactgataag gccattcggg
     9901 agtatttcga gaaggtttta cccgattttg atagggagag ggtttatacc agcgatatta
     9961 aaaaaattct tagctggtac aaccagcttc aggcaaacaa tcttttagca attctcgatg
    10021 ttgaagaagc aaaacctgaa gagccggcag ctgaagagca tagcaacaca gcccaagatg
    10081 ctgaaagtta gcaataactt ttttacctat tgcttttcac tatgaaaatg gccctagcag
    10141 ggccattttt acttaagttt tagtattcat agacaagatt aaagccaaaa ctcctgtcaa
    10201 gtccgggtaa aggggcatgt gagcccttat agggctgggg aaatacaaac gattccccca
    10261 gcatattgtt tacctcaata gttgcagtaa gaccctttag cactatattc ttaatgttta
    10321 cattgcagcc aagcataaaa agagggttca gtttttcgag tatatccatt ccatttcctt
    10381 ccctttgggt aaaggcgtaa cgttttccaa agtaagatcc tgacaacgtg agagataaac
    10441 ggttgtttac cctgaagtta aaaccaccgt taagccttag gggcgaaaat cctaaaaagt
    10501 aactttccct gtccggtacc ctgtaaaggc tatcggtctt ggaatgttta cgcatgtagt
    10561 aggcgaatgt agcaaatgat gaaattttag tgctgatatg cctaaaatcg gcttcaatac
    10621 ctcttgtgcc aattgtacct tcatttttat agtaacctac tcctgttata tcatcattac
    10681 ccaagttgta aacaattacc ttatcgaaag atacatcgaa aatgttaaag gtaatgtagg
    10741 attgaaaagg cattttaaac cctatagcca cttcgtagct ggttcctttt tcaggcttga
    10801 tttccggatt gccttgtggc aaccggttgg gaaggattcc tccggggacc ctaaatgact
    10861 ggctcaccat taccttgaag tgcataaaat caaaaacctt ggcaatgcct actcttggta
    10921 caaacgagct gccgtactcg ctggaaaaat cgtacctggc tccaagagta aagtttccta
    10981 cacggctatc gtgttgtaag agcccataaa tagccgtatt tgtgtaggac agctgctctt
    11041 tgccattcct aaatgtttcc tcgtaaaccg tgtcaaccag ctcgggctgg ttaaggtgat
    11101 catgtttgag ctcggctccg ccaacaatgg tcagcttatt ttttgtgtag cttgttgaaa
    11161 ttcctcctaa aagcccatca aatgttttgt tgttgttata cccatagcca gctcccaggg
    11221 cagtgagttt ccagggctgc tgccatttgt aagagatttt tggagtaatg gtaaggttgc
    11281 cattaggctt aaaatcataa gtagcttgaa ccagcgagct cttgaagctt tcggttaaag
    11341 gcggtcctgg gtagtagcta tcccagaggt cgatttgctg gtaggagtag tcatcaataa
    11401 acccaaccag gttgaagttt ttgtagttaa gcttcaggtc gagctggcgg gtgtaggtat
    11461 cgctagcatt cctcattgaa cgtgattgtc catggtagtc aacgtaatcc ctgtcgcttc
    11521 gcgaaccatt accgtagttt ccggatatac ctatgttaag gtagttttgg tgtacgcctg
    11581 taccaaagcc tacgttattt tgtgaaaatt gcctgcctgt gtggttggca gtgtaagaaa
    11641 cgtatcctcc cggtttatat acatccctgg tgatgatgtt cactacaccc ataccagcaa
    11701 aacctccata cattgcagag cccgggcccc ttatcacctc aattcgttct atattattca
    11761 acggaaagtg gttaccgaat gggacggatg cgaacatccc gtcgtttagc tccaacccat
    11821 ttaccataaa caaaacttta ccttcatgtg cccaaatacc ccgtactccc attcctacta
    11881 ctccttcaac atcaacccca aagtcgaaac ctggcacaaa aagttgaaaa agatctatta
    11941 ttccgtttac accgctgttt tttatatcct cactggtgat taccgtaagt attcccggtg
    12001 tatttctaat atttgttgct gttttagtag atatgctaac ctccaagttc agcagctcct
    12061 ccagcgagag gttaaaaatg tcttcttctt tattttgacc tttagccgaa gcagctagca
    12121 aaacaattag gcttacaact aaacatcttt tcatatgcca atcatatttg tttgttaaaa
    12181 tgaaggctta aaacttcgcc atttatagcc atcgtggttg atgtaaagat atttactttg
    12241 tgattgctta tttaataata tattgatagg caatatagcc ataattggag atagtatcaa
    12301 tggcactttg aaattttggt tcattcttcc aatctagtgg ctgcagctga aatggctact
    12361 cgttttccct taaaatgaag gctagcacgt catagaagct atcagctact gtaagatttt
    12421 taccaagctc atttcgggta tgagttgttg ccatggctac cacccgcatc cctgccgctg
    12481 tggccgcctg gattccggaa aaagaatctt cgaaaactaa acacccctca gggtgaacct
    12541 ttagtagctc ggaggcttta aggtagattt ctgggtctgg ttttccatgc tgaaccattg
    12601 atgcgtcaac aaccacatca aatctattct cgaggcgtaa tgacttctca ataaatttaa
    12661 agttagactt tggtgctgag gttgccacgg cgagtcttac tccgttttct tttaaataat
    12721 ctagcagctc gatcaaacca tcaacaggtt ttatgaatgg caagtaaata tccctataaa
    12781 tagcttcctt ttcctctccc agtagttcag cctcgtgggt tgaaatagac tttccggtta
    12841 gctcactaat tatctcctgg ttattttttc caaagtatgc cgtggtgaag gttttctcat
    12901 caaatttata gccatgacgc ctgcaaaact ctttccatgc attcagatgg tactggtggt
    12961 tattaaccaa cacgccatcc atatcaaaaa tggcggcatc aattgctttc gaaaatgttt
    13021 tcatcaaccc aaataattaa gaacactatc acctaaaatt attattaggc agaagctggt
    13081 cagccaatct atgctgttgt gcttgccgat agccctttca acaggctttt tttaccataa
    13141 attcattctg tagtgttaac gtgggaaaag aactattttt ttgtaaattt gtttcgtagt
    13201 atgaaaatcc gaagatagca tttatttagg tatatgaaga attatatgaa atccctagga
    13261 atagttttta tcatgatttt aagtccattg gttatgctag ggcagcatgt taaaatggat
    13321 aaagatggaa ttaagcatgg accaattatt ggcgctattc cagatacggt aaagctggga
    13381 gaaatttttt tagatgagct agacgatggc aacgggaagg tgcagcttac tgtggtgaac
    13441 cagggtaata aacctttaat acttaccagg gtttccggat gctgtggaac taatattaag
    13501 agttggccta aagcgccaat tttgcccaac caaaagggga cgattgaggt ttacttcaga
    13561 attgaaaatc gtcctcagcg cataagtcga actgtaacca tcgaatcaaa tgccgctaac
    13621 tccccggtgg taaaagttcc aataatcgga gtagttgttg aacggaaggc aaaaaatgag
    13681 ctaacccttt aaactattat tattttaaat gcttttgctt aaagtttgtt gaatttaatt
    13741 attgcacgaa taatgaagga gcgtttgcat agcacaacct tcgaccttcc ggttcaagag
    13801 cttcgccggg gttatagaag cgatgtgtat ttttggcgcg agaaggttgt gcttgaaagc
    13861 cataacctcc acccgcatgt taccatgcag gtttttcaga aaaatgaggc agtagtgtgt
    13921 ggcattgatg aggcagttgc ggtgctaaag gttgccagtg ggcgttactc cgactacgat
    13981 caggcgtata agctgtttga taagcttatt gagcttaagc ggatgggccg aaaagtattt
    14041 cttactgaca aaaagaaata cttacagctg gaggaggaga aaatgaaagt ttcccaggct
    14101 cttgataagt tgtgggttga cgggttttcc gatttgaaaa ttgatgcact ttgggatggc
    14161 gatgccattt c
//
LOCUS       DAOPHX010000062        14136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_5000_length_14136_cov_5.799588, whole genome shotgun sequence.
ACCESSION   DAOPHX010000062 DAOPHX010000000
VERSION     DAOPHX010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 14136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14136
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5000_length_14136_cov_5.799588"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..3102
                     /locus_tag="PK502_06060"
     CDS             157..3102
                     /locus_tag="PK502_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPH00413.1"
                     /translation="MGIHMIEDFEKLATRLKGEIRWDTPTLLQYATDASAYREIPKAV
                     VWPKGKSDLQELVSFAQQQNLTLTPRTAGTSLAGQVVSNGVIVDLSKYWGKIIEVNQQ
                     EHYVWVEPGVILDELNLYLKPYGLFFAPETSTSNRCMIGGMVGNNSCGSHSLIYGSTR
                     DHLLSVRIMLDDGEVHEFSNLTYIEFEAKCKLSNREGEIYRFFEQLLGKEGNRKLIAE
                     NSPDPSLRRRNSGYALDHLATNTFFNGSTNPFNLAKLVAGSEGTLGIVTEIKLNLVPL
                     PPKEKAVVAVHLEKREQAFRANLIALKYYPAAVELMDNVILNCTKGNIEQQKNRFFVQ
                     GEPGAILMVEFYGSNQQEIEEVANEMENEMRLNGYGYYFPIIWGNETTKVWALRKAGL
                     GVLSNVIGDAKPVSLVEDTAVAVEKLPDYMEEFAGIMGKYNLDCVYHAHIGTGELHLR
                     PVLNLKDPNDVELFRKVGLEVASLVKKYRGSLSGEHGDGRLRGEFIPFMYGNEVYQMM
                     LQVKKTFDPNNIFNQGKIVNTPPMNTSLRYQPGIPTREIATIFDFSGTQGIIRAIEKC
                     NGSGDCRKTHRMGGTMCPSYMATLNEANTTRARANLLREYLTNSPKDNPFDHKELYEV
                     LDLCLMCKGCKSECPSSVDMTKLKAEFLQHWYDIHGIPFRNKMVAYITQIYRLGAIFP
                     KFTNLLLSSKVTSFAIKNMLGFAQSRALPKLSSITLRRWQAKQKNSNKYPNGIVYLLP
                     DEFTNFNESHIGVKAAELLNYLGYEVRIPDILESGRTYISKGLIRTAKRIANNNIDKL
                     KGIIGEDTPLVGIEPSAILSFRDEYPDLADDRNTKASKELAMNSLLFEEFFMREASKG
                     KIVKEHFKQGNLRIKLHGHCQQKAVASTQATKAMLSFPEGYEVSEIPSGCCGMAGSFG
                     FEKEHYELSMKIGELVLFPEIRKSSETDVIVAPGTSCRQQIYAGTGRYALHPIEVMWQ
                     AANISSQRS"
     gene            complement(3115..3987)
                     /gene="sucD"
                     /locus_tag="PK502_06065"
     CDS             complement(3115..3987)
                     /gene="sucD"
                     /locus_tag="PK502_06065"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPH00414.1"
                     /translation="MSILVGKNSRVLVQGFTGSEGTFHATQMIEYGTNVVGGVTPGKG
                     GQKHLDRPVFNTVDDGVKTTGADVSIIFVPPAFAADSIIEAAAAGIKLVVCISEGIPV
                     ADMVRVKYFLEDFPVTRLIGPNCPGIITPEEAKVGIMPGFIHKKGHIGVVSRSGTLTY
                     EAVDQLTKLGLGQSTGIGIGGDPIIGTTTLDAIKLFMNDPDTHGIVMIGEIGGSMETE
                     AAQWVKIHGTKPVVGFIAGQTAPKGRKMGHAGAIIGGTADTAEAKMNTMRECGIHVVE
                     SPANIGRAMKDALK"
     gene            4265..5728
                     /locus_tag="PK502_06070"
     CDS             4265..5728
                     /locus_tag="PK502_06070"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPH00415.1"
                     /translation="MAKVNIKLENLSEMFPNSWTDEQKAKGNTLFLKYLSEKAHKFYG
                     GKVQTVPKAPVPGFNWFNVWYTPGVSKVSTTIRDNNDTSFELTNRGNLVAVVSDSTRV
                     LGDGDCTPPGGLGVMEGKAFLMKYLGGVDAVALCIDSKGKDGKNDPEKIIDFVKMCQH
                     SFGAVNLEDISQPNCYRVLDVLREECIIPVWHDDAQGTACVTLAGLLNALKLANKKMS
                     NAKMVLFGAGAANTTIARLIIADGGDPHNMVIFDSHGGLHKNREDIKADHRFYRKWEL
                     CEQTNPQCINTMEEAMRNADVLISVSTPGPDTIKQEWVKLMAPKSVVFACANPVPEIY
                     PYAAKEAGAYIVATGRGDFPNQVNNSIGFPGILKGALIARASKITDSMAIAAAHSLAG
                     FAEKRGISPENIVPTMDEADVFPVEAADVAVQAVKEGVARRQVSWEEAYRIAKKDIES
                     SRSMTKALYDNGYIANPPAEMLEDALLWTIQQVEAKK"
     gene            6073..7974
                     /gene="ftsH"
                     /locus_tag="PK502_06075"
     CDS             6073..7974
                     /gene="ftsH"
                     /locus_tag="PK502_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPH00416.1"
                     /translation="MTKREKYSWVYWLSILAFVIALQLLVFPDTQGPREFTYSEFIDS
                     VNAGKVERIVFFEDKIIGQLKSGEETGQKSITQAPTAPWRLRIPSIEQQVKRQFVVNR
                     IPNMNEEYLISILTGTNVSFKGHVENNVLRNFFLNWLLPLLIFMALWGFFFKKMGSKN
                     PMLEIGKNKAQIHAEKPKNPITFKDVAGVDEAVDEVRELVDFLKYPEKFTRLGGKLPK
                     GVLLVGPPGTGKTLLAKAVAGEADVPFFSLSGSDFVEMFVGVGAARVRDLFNQAKVHA
                     PCIIFIDEIDAIGKSRAQNAAYSGGYDERENTLNQLLVEMDGFDATSGVIIIAATNRP
                     DVLDPALLRPGRFDRQVLVDKPDMKGREAIFKVHSRNLKLSSKVDLSRLAAQTPGFAG
                     AEIANVCNEAAILAVRNNRDEVTMSDFEAAIERVVAGLEKKNKLINEKERQIVAFHES
                     GHAVVGYFTPGAEEVQKVSIVPRGIGALGYTLQMPLEDRYLMSKSELLGKIKGLLGGR
                     AAEEVIFGEVSTGASNDLERASQLARSMIIVYGMSSKLPNISLVNKSNPGFLGQPSGI
                     ERRSEYVEKLIDEEVNEIINTCYHEAKMLLKEKKHLLDKMASILLKQEVINHDEIISI
                     LGDRTPIED"
     gene            complement(7998..8444)
                     /locus_tag="PK502_06080"
     CDS             complement(7998..8444)
                     /locus_tag="PK502_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPH00417.1"
                     /translation="MRDDFNEDLLNLPFNIHRTSVAIRGEITRRLSEAFGDDFTVDYW
                     YILTLLFSREPLSQAHLAQISGRDKGSLSRTLACMERLDLVVRLPNPDNSRSELVSST
                     KEASNFKPKAESIILDVLSEALSSLRPIEVIELNRMLAAINEKIQP"
     gene            8823..11237
                     /locus_tag="PK502_06085"
     CDS             8823..11237
                     /locus_tag="PK502_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPH00418.1"
                     /translation="MEYRLYFSIPNRLSSPVPGRAELNFELINRKDLVIDFMTEATNL
                     QSITVNNRLLSIRVKNGHINIPSKYLLTGQNKVNIDFVSCSPSLNRKADYLYTLLVPD
                     RASTVFPCFDQPDLKAAFNLTLEIPKEWVAVANAPLGKIIESDTSTVYVFEKTKPVST
                     YLFAFACGKFKVASQPTRYGEMAIYYRDNDSAKVNRNIEAIYELHTQSLGWMENYTAI
                     PYPFSKLDIVLIPDFQYNGMEHPGAIYYRESSILLEGDPSENKLLNRANLIAHEVSHQ
                     WFGDLVTMRWFNDVWLKEVFAGLMADKIVNPQFPEFNHQLSFLLSHYPRAYSVDRTAG
                     ANPIRQELDNLLNAGSLYGDIIYHKAPIALMQLEMAMGVSAFQEGLRKYLNTYSMGNA
                     SWDELIDILDPLTSLNLSVWSKAWIESVGMPTLKATLNDSMYVVKKIGVQLPMVYSVE
                     VLCGSNVNEAKPIQHIQPEVSTLFASSCELERVIVPNADGLGYGMFVPDSQSLKLLCD
                     SSFRIADPVTRSASLISANELFLNGKLDGNTYFNMLLASLARENEPQVRSYILASIET
                     YWWTFLADSARENLSQQVEIQFSKLLLNSSIPADERKSIFKTYARIAVSSNAAEELYG
                     IWSGNKSLPGLTFTETDLTFLAYELAVRNVHNADSILDLQEKRIANPDLLAKFRFVRR
                     ALSPDDALRDDFFHSLLIPENRRPEPWVAEALHYFNHPLRDSFASQYLRASLDLLPEI
                     QQTGDIFFPKTWLDAILWGKNSNLAAEIVSSWLNQHPNVPDNLKNKLIQSSDLLFRAV
                     DKRKKK"
     gene            complement(11320..13011)
                     /locus_tag="PK502_06090"
     CDS             complement(11320..13011)
                     /locus_tag="PK502_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HPH00419.1"
                     /translation="MNKIKPIMPVMLMIVLVAASSCTSNQKKMEKQMADFIAKVESKV
                     IPLSKEAAIASWDASISGKDEDYQRSEKAQVELVKIFADTSLFAELKSIKESGLVKDS
                     VLSRQMDILYLAFLRNQVDPTLLEKEIAMEANLEKKYSNFRAKIGSKQLTDNEIEDIL
                     ATSTQSSEVESAWKAHKKIGPEVAPDIIELVKVRNQIAQKLGYANYHTMNMVLSEQDP
                     DEVLALFDELDSLTSGSFSELKLEIDSYLSKRFGVSPDKLMPWHYQNRYFQEAPKIYN
                     VNLDQFYSDKDVVALTAKFYNGIGLNIDDIIARSDLYEKPGKNQHAYCTDIDRSGDVR
                     VLCNVKPTYGWMNTLLHENGHAVYSKYNDPAKPYFLRDAAHSFTTEAIAEMMGRLASN
                     PQWMLDMGIITPEQKEQIASECLKSLRLEQLVFSRWAQVMYRFEKEMYANPDQDLNSL
                     WWSMVEKYQLLKKPEGRNEPDWASKIHVALYPCYYHNYLMGEMLASQLNAYIAANVLK
                     ISDFNAASFVGHPEVGAYLKEKVFVPGCLYPWNEMIEKATGEKLTAKYYALQFVK"
     gene            complement(13244..14125)
                     /gene="dapA"
                     /locus_tag="PK502_06095"
     CDS             complement(13244..14125)
                     /gene="dapA"
                     /locus_tag="PK502_06095"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009160945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPH00420.1"
                     /translation="MQNDRFKGLGVAMVTPFSSSKAIDYSAADRLLEYLVDGGVDYLV
                     VMGTTGEASTLTDDEKHELIAHVVEKNNGRLPIVLGVGGNCTSSVISSIKHFNLKGVD
                     AILSVTPYYNKPTQKGLEMHFRAVAESSPLPLILYNVPGRTGVNMSAQTTLTLANEVP
                     NIIGIKEASGNLNQMAYILRDRPDNFLVISGDDGLALSQMAMGADGIISVVGNAVPGR
                     FAEMVHFAQKGKFAEAAVIHLKLVELIDALFVEGNPGGVKAALSILGIIDNNLRLPLV
                     PVGEATLQKLNMLLKSL"
BASE COUNT         4132 a         3080 c         3141 g         3783 t
ORIGIN      
        1 agctactgtt taaaaaatgc tgtaacatgg ctatttgcga cacaagaaca gtaaaatgtt
       61 ctacaacatg ctaaaatgac caataaaata tgttgaataa aaatcgtata ggcaaaaatt
      121 tttcagctag gtttgcaatg ccttaaaaga atgtagatgg gcatacatat gattgaagat
      181 tttgaaaagc tggctacccg gcttaaaggt gaaatacgat gggatacccc taccctgctt
      241 cagtatgcaa cagatgcctc agcttacagg gagattccaa aagcggtggt gtggcctaaa
      301 ggcaaaagcg atttgcagga gcttgtttct tttgcccaac agcaaaacct aacgctaaca
      361 ccccgaactg ctggcacttc gctagccgga caagttgtga gcaatggtgt aattgttgat
      421 ctgtcgaagt actggggaaa gattatcgaa gtaaatcagc aagaacacta cgtttgggtt
      481 gaaccaggag taattcttga tgagctaaat ctatacttaa aaccctacgg tttgtttttt
      541 gctcccgaaa cctctacttc taaccgttgt atgattggtg gaatggttgg gaacaacagc
      601 tgtggttctc attcattgat atatgggagt acgcgcgacc acctgctttc tgttaggata
      661 atgctcgacg acggagaagt tcatgagttt agcaacctaa cgtatatcga gtttgaagct
      721 aaatgcaagc tgagcaatcg ggagggcgaa atttaccggt tttttgagca gctgcttgga
      781 aaagaaggaa acagaaagct tatagctgaa aattctcctg atccttcact tcgccgaagg
      841 aattcagggt atgcacttga tcacctggca accaatacct tttttaatgg gagcaccaac
      901 cccttcaatc tggctaaact tgtagccggt agcgagggaa cactaggcat agtaaccgaa
      961 ataaagctga accttgttcc gctaccgccc aaggaaaaag cagttgtggc agttcacctt
     1021 gagaaaaggg agcaagcctt tagggctaat ctcattgcat tgaagtatta cccggctgct
     1081 gttgagctaa tggacaacgt gattttgaac tgcacaaagg gaaatattga gcagcaaaag
     1141 aatagatttt tcgttcaggg tgagcctggt gcaatactga tggttgagtt ttatggtagt
     1201 aaccaacaag agattgagga agttgcaaat gaaatggaaa atgagatgcg cctcaacgga
     1261 tatggatact actttcccat aatttggggg aacgaaacaa caaaagtttg ggctctccga
     1321 aaagccggat taggtgttct ttcgaatgtt ataggtgatg caaagccagt atcacttgta
     1381 gaggatacag ccgttgcggt tgaaaagcta cctgactaca tggaggaatt tgcaggtata
     1441 atgggcaagt acaatctcga ttgcgtttac catgcccata ttggcacggg cgagctccac
     1501 ctccggcctg ttctcaacct caaagatcca aacgatgtcg aacttttccg taaggttggg
     1561 ctagaagttg cttccctggt taaaaagtac aggggatcgt tgagcggaga gcatggcgat
     1621 ggacgactta gaggggagtt tatcccgttt atgtatggaa acgaggttta ccagatgatg
     1681 ctccaggtga agaaaacttt tgaccctaac aacattttca accagggcaa gattgttaac
     1741 actccaccca tgaataccag cctgcggtat caaccgggaa taccaactcg ggaaattgcc
     1801 acaatttttg attttagcgg tacccaaggt attattcgtg ccattgaaaa atgtaacggg
     1861 tccggtgatt gccgtaaaac acaccgcatg ggtggaacca tgtgccccag ctacatggct
     1921 acattaaatg aggcaaacac caccagggca agagcaaacc tgcttagaga gtacttaacc
     1981 aacagcccta aagacaatcc ttttgatcac aaggagctgt acgaagtttt agacctctgc
     2041 ctgatgtgca agggttgtaa aagcgaatgc ccttcaagtg ttgacatgac aaagcttaaa
     2101 gctgaattcc ttcagcactg gtacgacatt catggcattc cgttccgaaa caaaatggtt
     2161 gcctatatca cccaaattta tcgtttgggg gcaatttttc ccaaatttac caatctattg
     2221 ttatccagca aggtaacctc attcgctatc aagaacatgt taggttttgc tcaaagcaga
     2281 gcactgccta aactaagtag tataactctg cgcagatggc aggcaaaaca gaagaactca
     2341 aataaatacc ctaacggtat agtttacctg ttgcccgatg agtttacaaa cttcaacgag
     2401 tcgcatattg gagtaaaagc agctgagctg ttaaactatc ttggctacga agtacgtatt
     2461 cccgatatac ttgagagtgg cagaacttac atttcaaaag gccttatcag aacagccaag
     2521 cgcatagcca acaataatat tgataagctt aagggaataa taggtgaaga cacaccgctg
     2581 gtaggcattg agccttcagc catacttagc ttcagggatg agtaccccga cttggccgac
     2641 gaccgtaata caaaggcttc gaaggagctg gccatgaatt ccctgctatt cgaggaattc
     2701 tttatgcgag aggcgagcaa agggaagata gttaaagaac actttaagca gggaaatttg
     2761 agaattaaac ttcatggcca ttgccagcaa aaagcggttg cttccaccca ggcaacaaaa
     2821 gccatgctat cattccctga agggtatgaa gtaagcgaaa taccaagcgg ttgctgcgga
     2881 atggctggtt cctttggatt tgaaaaggag cactacgaac tttcaatgaa gataggcgaa
     2941 ctcgtactct tcccggaaat tcgtaagtct tcagaaaccg atgtaatagt agcacccggg
     3001 actagctgca ggcagcaaat ttatgctgga acaggtcgtt atgcacttca tcccatagag
     3061 gtgatgtggc aagctgccaa cattagcagc cagcgttcat aggctaccac cccactattt
     3121 aagtgcatcc ttcattgccc ggccaatgtt ggcaggcgat tccacaacat gaatgccgca
     3181 ttcacgcata gtattcattt tagcttcggc agtatcggca gttccaccaa taattgcacc
     3241 ggcatgcccc attttccttc cttttggcgc agtttgcccc gcaataaagc caaccacagg
     3301 cttagttcca tgaattttta cccattgtgc cgcctcggtt tccatgcttc ctccaatttc
     3361 gccgatcatc actatgccat gagtgtcagg gtcgttcatg aatagcttaa ttgcatccag
     3421 ggttgtagtg ccgattatgg gatcaccgcc aataccaatt cctgtacttt gccccaatcc
     3481 cagcttggta agctggtcaa cagcctcgta ggttagggtg cccgaacggg atacaacacc
     3541 gatatggcct tttttatgaa taaagcccgg cataattcca acctttgcct cctcgggagt
     3601 aataattccc gggcaattag gaccaattag ccgtgtgaca ggaaaatctt caagaaagta
     3661 ctttaccctc accatatcgg caaccggaat accctcgcta atacatacaa caagtttgat
     3721 accagctgcc gctgcttcaa taatgctatc ggccgcaaaa gcaggaggta caaaaattat
     3781 tgaaacgtct gctccggttg tctttactcc atcgtcaaca gtgttaaata ccggcctgtc
     3841 cagatgtttt tgtcctcctt tccctggtgt aacccctccc accacattag taccatactc
     3901 aatcatctgg gtggcgtgaa aggtgccctc gctaccggtg aagccctgaa ccagcaccct
     3961 ggaattttta cctacgagaa tactcatctt tcctcatttt taggattaca actcctatca
     4021 aaaattacct taacctgcat gcaagaagtt aaattcaagt aaaaaaatta tcgaatcaaa
     4081 cctagccaat ttataggaaa actgcaacaa ttttactaac aaatctaacc ttttatatac
     4141 attacaaacg tttacgataa aatttttcag catttccgaa tagctatcac aaaatgtttg
     4201 cgtaacttgg taagggtatt ttataaaagg gcaatgcata caaatcatta aaatttaact
     4261 ggttatggct aaagtaaaca taaaactaga gaaccttagt gaaatgttcc caaacagctg
     4321 gacggatgag caaaaagcta agggtaatac gctgttctta aaatatttga gcgaaaaagc
     4381 ccacaagttc tacggtggca aagtacaaac agtaccaaaa gctcctgttc ccgggtttaa
     4441 ctggtttaat gtgtggtaca cccccggagt atcgaaagtt tccaccacca ttcgcgacaa
     4501 caatgatacc tcttttgagc ttaccaacag gggcaatctg gttgctgttg ttagcgattc
     4561 tacccgtgtg ctaggcgatg gcgattgcac gcctccgggt ggtctggggg ttatggaggg
     4621 aaaggcattt ttgatgaagt atcttggagg agttgatgct gttgcacttt gcatcgacag
     4681 caagggtaaa gatgggaaaa atgatcccga gaagattatt gattttgtta aaatgtgcca
     4741 gcatagcttt ggggcagtta accttgagga catatctcag ccaaactgct acagagttct
     4801 cgacgtgctt cgtgaagagt gcatcattcc ggtttggcac gatgatgcac aaggaacagc
     4861 atgtgttacg ctagcaggat tacttaatgc cctaaagctt gccaacaaaa aaatgagtaa
     4921 tgccaaaatg gtgcttttcg gtgccggagc agccaacacc accattgccc gtttgattat
     4981 tgccgatggc ggtgatccgc acaacatggt tatttttgac tcacacggcg gcttgcataa
     5041 aaatagggag gatataaagg ctgatcacag gttctaccgt aaatgggaac tctgtgaaca
     5101 aacaaaccca caatgcatca acaccatgga agaagctatg cgaaatgccg acgtgcttat
     5161 ttcggtttct actccaggtc ccgacaccat aaagcaggaa tgggttaagc taatggcacc
     5221 caaatcggtt gtttttgctt gcgccaatcc cgttccggaa atttacccct atgctgccaa
     5281 agaagcaggc gcttacattg tggctaccgg acgcggggac ttcccgaacc aggtgaacaa
     5341 ttcgattggt tttcctggaa ttttgaaagg ggcattgatt gcccgcgcat caaaaattac
     5401 tgatagtatg gctattgccg cagctcactc attagctggc ttcgctgaaa aaaggggtat
     5461 ttccccggaa aatattgttc caacaatgga tgaagctgat gttttccctg ttgaagctgc
     5521 cgatgttgcc gttcaagccg taaaggaagg cgttgcccga aggcaggtta gctgggagga
     5581 agcctacaga attgccaaaa aggatattga aagctcgcgc tccatgacca aagcacttta
     5641 cgacaacggg tacatagcca atccaccagc agaaatgctc gaagatgcgc tcctgtggac
     5701 tatacaacag gttgaggcaa aaaagtaaaa ggatgatgtt ttttgttcat cccttgattg
     5761 ggttggcaaa tgggttttac acgtcattta actcttcgag cgagcactat actatataca
     5821 tataataatg cccaaagaaa actcgggttg aggtttactt tttcccaatc ataggggtta
     5881 tagtatttct aagaatctct gaggcaattg aaatcttcat ccccaaacaa aagcatgaca
     5941 aaatggagta cagaaattcc tgtaacgctg taatagatgt caaaattaaa cctacagctt
     6001 tttcctatgc tgaaaaattg tcatgattag taattggttc agttttggat attgcgaaaa
     6061 aaaaaactca acatgacaaa acgtgaaaaa tattcgtggg tatactggtt aagcatttta
     6121 gcttttgtaa tagctttaca gctgctagta tttcctgata cccaagggcc aagagaattc
     6181 acttatagtg aatttatcga ttctgtcaat gcaggcaaag ttgagcgaat tgtttttttt
     6241 gaggataaga taattggtca actcaaaagc ggtgaggaaa caggtcaaaa atcaataact
     6301 caggcgccta ctgcaccatg gaggcttcgc attcccagta ttgagcagca agtaaaaaga
     6361 caatttgtgg tgaataggat acccaacatg aatgaagaat accttataag cattctcacc
     6421 ggaacaaatg ttagctttaa agggcatgta gagaataacg ttttaagaaa cttttttctg
     6481 aactggcttc taccgctgct tatttttatg gccctatggg ggttcttttt caaaaagatg
     6541 ggatcgaaaa atccaatgct tgaaattggt aagaataaag cgcaaataca tgccgaaaag
     6601 ccaaaaaatc ctataacatt caaggatgta gcgggggttg atgaggcagt tgatgaggtt
     6661 agagagctgg ttgactttct gaagtacccc gaaaaattca ccaggttagg agggaagttg
     6721 cctaaagggg tgttgcttgt tggtcctcca gggaccggta aaactctcct ggctaaagcc
     6781 gtagctggcg aagccgatgt gccctttttt agcttaagtg gttcggactt tgttgaaatg
     6841 tttgttggag ttggcgccgc cagagtccgc gatttgttca accaggcaaa agttcatgct
     6901 ccttgtataa tattcattga tgaaattgac gctataggta aaagcagggc tcaaaatgct
     6961 gcgtatagcg ggggatacga tgaacgggaa aacaccctca accagctact ggttgaaatg
     7021 gacggttttg atgccacatc aggagttata attattgcag caaccaaccg gccggatgtt
     7081 ttggatcctg ccctgctaag accaggaaga tttgaccggc aggttttggt tgataaaccc
     7141 gacatgaaag gtagggaagc catttttaag gttcattcca ggaaccttaa actcagctca
     7201 aaagttgatt tatcgaggct tgctgctcaa acacccggat ttgcaggtgc agagattgcc
     7261 aacgtatgta atgaagcagc cattcttgcg gtaaggaaca atagagatga agtaaccatg
     7321 agtgattttg aggcggcaat agagcgggtt gttgctggct tagaaaagaa aaataagctc
     7381 attaatgaaa aggaaaggca gattgtagcc ttccacgaaa gcggtcatgc agttgtaggg
     7441 tattttaccc ctggtgcgga ggaggttcaa aaggtgtcta ttgtaccccg gggaataggc
     7501 gcgctgggat ataccctgca gatgcccctg gaggatagat acctgatgtc aaaatccgaa
     7561 ctgttgggca aaatcaaagg actactgggt ggacgtgccg ccgaagaggt aatatttgga
     7621 gaggtatcaa caggggcttc aaacgatctg gaacgagcat cacaactcgc ccgaagcatg
     7681 attattgttt acggaatgag cagtaagctg ccaaacattt cgctggtaaa caagtcgaac
     7741 cccggctttc tgggacagcc ttccggtatt gaaagacggt cagaatatgt ggagaaacta
     7801 atagacgagg aggttaatga aataattaat acctgttacc acgaggcaaa aatgttacta
     7861 aaagaaaaaa agcatcttct ggataaaatg gcctccattc ttctaaaaca ggaggtgatt
     7921 aatcacgatg aaatcattag tattcttgga gatagaacac caatcgagga ttaaagcaaa
     7981 tgtttaccgc ttaacttcta tggctgaatt ttttcattta ttgccgccaa catccggttg
     8041 agctctatca cctcaatagg cctcagggag ctaagagcct cagaaagaac atccaaaata
     8101 atgctctcgg ctttaggctt aaaattcgag gcttctttcg tagagctaac cagctccgat
     8161 cggctattat cagggttggg aaggcgaaca accaaatcga gacgttccat acatgccaag
     8221 gttcgtgaaa gtgaaccctt atcccttcct gatatttggg ccaaatgagc ctggctgagt
     8281 ggttctcttg aaaacaaaag ggtaagaatg taccagtaat caacggtaaa gtcatcacca
     8341 aaagcttccg atagccttcg ggtaatctcg cctcttatgg caaccgatgt gcgatgtatg
     8401 ttgaatggca ggtttaaaag atcttcatta aaatcatctc tcataaacaa gtgaatattg
     8461 tgttagccaa gccgtgaata gtccgcttaa atttaataaa atattcacaa cttattttcc
     8521 actccagcat ttatcggcat tcgtaaattt gtaacaccat tagtttaaac caataataag
     8581 gttttagctc taatttaatg caagcagcaa gtaataaaat gcttcacaat aaagttttta
     8641 tatagcatta acggttttta ccttactaga tgtttaacca ccattcttta aaattgatga
     8701 atttttatag gagcttatta accccattag taggtatttt gattttatcg agctgtgtta
     8761 cccaactaac gcccggggtt tcgaaatcac ttgccgactc tcgcagtgca aacataaaaa
     8821 atttggaata ccggttatat ttttcgatac ccaaccggtt aagttcacct gttccgggcc
     8881 gagccgagct taactttgaa cttatcaacc ggaaagattt ggttattgat ttcatgaccg
     8941 aagccaccaa cctgcaatca attaccgtaa acaaccggct attgagcata agggttaaaa
     9001 acggccacat caatattcca tccaaatacc tgttaaccgg gcaaaataaa gtaaatatag
     9061 attttgtatc gtgttcccca tcgctcaatc ggaaagccga ctacctttac acgcttctgg
     9121 ttcccgacag ggcatcaact gttttcccat gctttgacca gcccgacctg aaggcagctt
     9181 tcaacctaac tcttgaaatt cctaaggaat gggttgcagt ggcaaacgcc ccattaggca
     9241 aaattattga gtcggataca tcaaccgttt atgtttttga aaaaaccaag ccagtaagca
     9301 cctacctgtt tgcttttgca tgtggtaagt tcaaagtagc ttcgcaacca accagatatg
     9361 gcgagatggc catttactat cgcgacaacg attctgccaa ggtaaatcgt aatattgaag
     9421 ccatttacga gttgcacacc caatcgctgg gctggatgga aaactatacg gccattccat
     9481 atccattcag caagctggac atcgtgctta ttcccgattt tcagtataat ggaatggagc
     9541 atcctggagc aatctactac cgggaatcaa gcatcttgct cgaaggcgat ccgtcagaaa
     9601 acaagctgct taatcgcgca aaccttattg cccatgaggt ctcacaccag tggttcgggg
     9661 acctggttac catgcgatgg ttcaatgatg tttggctaaa ggaggtattc gcaggcttga
     9721 tggccgataa aattgtcaat ccccagtttc ccgagttcaa ccaccagctt agctttctgc
     9781 tatctcacta cccacgtgct tattcagttg acagaacggc tggagctaac cctatacggc
     9841 aggaacttga caacctgctt aacgccggct cgctttatgg cgatatcatt taccataaag
     9901 ctcccattgc ccttatgcag cttgaaatgg ctatgggtgt tagtgctttt caggagggat
     9961 taagaaagta tctgaatacc tatagcatgg gaaacgccag ctgggatgag ctgatagaca
    10021 ttcttgaccc gttaacctca ctaaatttaa gtgtatggag taaggcttgg attgaaagtg
    10081 ttggtatgcc aacccttaaa gcaacgctaa acgatagcat gtacgtagta aaaaaaatcg
    10141 gcgtacagct tccaatggtt tacagcgtgg aggttttatg cggtagcaat gtaaacgagg
    10201 ctaaacccat tcagcatatt caacctgaag tttctacact atttgcaagt agctgtgagc
    10261 tagaaagagt gattgttcca aatgctgacg ggcttggtta cgggatgttt gttcccgatt
    10321 cgcagtcgct taaactactg tgtgatagtt cttttcgaat agctgatccg gttacccgtt
    10381 cagcatcctt gatatcggct aacgagctat ttttaaacgg caaacttgat ggaaatacct
    10441 acttcaacat gctacttgcc tcacttgcaa gggaaaacga acctcaagtg cgcagctaca
    10501 tccttgccag tattgaaacc tactggtgga cttttctggc ggatagcgcc agggaaaatt
    10561 tatcgcaaca ggttgaaatt caattctcca agttgttact aaacagttca atcccagctg
    10621 atgagcgaaa atcaattttc aagacatacg ccaggatagc tgtgtccagc aatgccgctg
    10681 aagagctgta tggaatatgg agcggtaata aatctttacc aggtttaacc ttcacggaga
    10741 ccgatttaac ctttctagcc tacgagctag ctgttagaaa tgtgcataat gccgacagta
    10801 ttctggatct acaggaaaaa agaatagcca accccgacct gcttgcaaaa ttccgatttg
    10861 ttcgacgcgc cctaagtccc gatgacgcct tacgcgacga ctttttccat agcttgctga
    10921 ttcctgaaaa ccgacgacct gaaccctggg tggctgaagc ccttcactac tttaaccatc
    10981 ccttaaggga tagttttgcc agccaatacc taagggcttc attggattta cttcccgaga
    11041 ttcagcaaac aggagatatc ttcttcccca aaacttggct tgatgccatt ctatggggta
    11101 aaaacagcaa cctagctgct gaaattgtaa gcagctggct aaaccagcac cctaatgtac
    11161 ccgataacct gaaaaataag cttattcaat cgtcggattt gctattcagg gctgttgata
    11221 aaaggaagaa aaaatagttt ccacaaacta accaaaaaaa aggctactcg ctgcccggag
    11281 gcagaggtag tcttttaaaa ggatatgctc ctaatctgct tacttcacaa actggagggc
    11341 atagtacttt gccgttagct tttcgccagt ggccttctca atcatttcgt tccagggata
    11401 caggcatcct ggcacaaaaa ccttttcctt gaggtatgca cccacttcgg ggtgtcccac
    11461 aaaactagcc gcattaaaat cactaatctt tagcacgttg gcagctatgt aggcattaag
    11521 ctgtgaggca agcatttctc ccatcaagta attatgatag tagcatgggt agagggcaac
    11581 atgaattttt gaagcccaat cgggttcgtt acgtccttca ggttttttca acagctggta
    11641 cttttcaacc atgctccacc aaagggaatt caaatcctga tcgggattgg cgtacatctc
    11701 cttttcgaaa cggtacatca cctgagccca gcggctaaac acgagctgct caagcctaag
    11761 ggattttagg cattcggaag caatttgttc tttttgttca ggggtgataa ttcccatatc
    11821 gagcatccac tgggggttgc ttgccagacg tcccatcatt tcggcaattg cctcggttgt
    11881 aaagctatga gcggcatctc ttaggaagta aggttttgcg ggatcgttat acttggagta
    11941 aacggcatgg ccattctcat gcaaaagggt attcatccaa ccataggttg gcttcacgtt
    12001 gcaaagcacc ctaacatcgc ccgaacggtc aatatccgtg caataggcat gttgattttt
    12061 accgggtttc tcgtagagat cggaacgagc tattatgtcg tcaatgttaa ggccaattcc
    12121 gttgtagaat ttggctgtaa gtgcaaccac atccttgtcg ctgtaaaatt ggtcgagatt
    12181 aacattgtaa attttaggag cttcctgaaa gtaccgattt tggtaatgcc agggcatcaa
    12241 cttgtcgggg gaaactccaa agcgtttaga caagtaggag tcaatttcga gtttaagctc
    12301 ggaaaacgac ccagaggtta agctgtcgag ctcatcaaaa agtgccaaca cttcgtcggg
    12361 atcttgttca gagagaacca tgttcatggt gtggtagttg gcatagccca atttttgggc
    12421 aatctggttg cgtaccttaa ccagctcaat gatatcgggg gcaacctcgg ggccaatttt
    12481 tttgtgcgcc ttccaggcac tctccacctc gctgctttgg gtcgaagtgg caagtatgtc
    12541 ttctatttcg ttatcggtta gctgcttgct gccaatttta gccctaaagt tggagtactt
    12601 cttctcaagg ttggcctcca tggcaatttc tttctcgagt agggtcgggt caacctgatt
    12661 acgaagaaag gccaggtaaa gaatgtccat ttgacgagaa agcaccgaat cctttaccaa
    12721 tccggattct ttaatactct taagttctgc aaaaagcgag gtgtctgcaa aaattttaac
    12781 cagctcaacc tgggctttct ccgatcgctg gtaatcttca tccttacccg atatggaggc
    12841 atcccacgaa gctatagcag cttctttgga taagggaatt accttcgact caacttttgc
    12901 aataaagtcg gccatttgtt tttccatttt tttctgatta gatgtgcatg atgaggcagc
    12961 tacaagcaca atcatcagca tcactggcat aataggtttt attttattca taaggcaata
    13021 attttgttga ctcaaaatta gtaaaccttt aaacgatata taaccacaat ataaaattat
    13081 agcgtaaaac ttgatgtgtg aaccggcagc ctatcagcaa attcaaaata ataagttggt
    13141 tgtgttgccg aactataaac tccgtagtaa aatagaagtt aatggacacc aacaagaagt
    13201 tagcgggtgc ggtcttgtag ctataaaaac attagctcgg ctgttaaagg cttttcagca
    13261 acatgttaag tttctgaagt gtggcttcgc caaccggaac aaggggtaac cgaaggttgt
    13321 tgtcgataat gccaagtatt gacaatgccg ctttaacccc tccgggattt ccctcaacga
    13381 aaagagcgtc aatcagctcc accagcttca ggtgaatgac ggcagcctca gcaaacttgc
    13441 ctttttgagc aaaatgaacc atttcggcaa aacgtcccgg cacggcattg cccacaaccg
    13501 aaataatacc atcagcaccc atggccattt gtgataatgc caagccatcg tcgccggaaa
    13561 ttaccaaaaa attatcgggt ctatccctca ggatgtacgc catttggttg aggtttcctg
    13621 aagcttcttt tataccgata atatttggga cctcgttggc cagggttagc gtggtttggg
    13681 cactcatatt aactcccgtt ctacccggaa cattgtagag aatcaggggt aatggagagc
    13741 tttcagccac agctctgaag tgcatttcca accccttctg ggttggcttg ttgtagtagg
    13801 gcgtaaccga aagaatggca tcaacaccct ttaggttgaa gtgttttatg gagctaatca
    13861 cgctcgaggt gcagttaccg ccaacaccta aaacaatggg taacctgccg ttattttttt
    13921 caactacatg cgcaattaac tcgtgctttt catcgtctgt cagggtcgag gcttcaccgg
    13981 ttgtccccat cacaaccagg tagtcaactc ccccgtcaac caaatactcc agcaacctat
    14041 cggcagcgga gtagtcaata gctttgctgc tgctaaaagg ggttaccatg gcaaccccta
    14101 atcccttaaa tctatcgttt tgcataatag tcggat
//
LOCUS       DAOPHX010000063        13624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_5271_length_13624_cov_6.164419, whole genome shotgun sequence.
ACCESSION   DAOPHX010000063 DAOPHX010000000
VERSION     DAOPHX010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 13624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13624
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5271_length_13624_cov_6.164419"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PK502_06100"
     CDS             complement(<1..192)
                     /locus_tag="PK502_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00421.1"
                     /translation="MMLTGLSLQDLPGRFPGVIIKNSKGYKEVGFRTRPPNSGFLISE
                     LARLPAELVGNLTELVRLPA"
     gene            269..2050
                     /locus_tag="PK502_06105"
     CDS             269..2050
                     /locus_tag="PK502_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPH00422.1"
                     /translation="MRPSGLKRQFSRTWHGFRRLGERRIMLILSVVVGVSSGLAAVLL
                     KNVLHVANVFVQESFPKDSANFLFFALPATGILLTIVFVRFLVKDDIGHGVSKVLYSI
                     SKQNGRIKPHNMYSSMVASTSTIAFGGSVGAEAPIVYTGAAIGSNVGNFFRLNYKNII
                     LLIGCGAAGAIASIFKAPLAGIVFTLEVLMLDLTTASIVPLLISAVSAATVSYFFLGR
                     EVVLAYDVVRPFALQKIPYYVLLGVFCGFISLYFIRVVMWIEKRFERITSIYRRWILG
                     AMILGTFTFLFPPLYGEGYEVLQALLDGRSSFIFNSSPFYEFQSNVWLLLVALLILIL
                     AKGVATAVTTGAGGVGGTFAPTLFIGGVAGFFVAKLLNQLGITVVSESNFALIGMAGA
                     MAGVMHAPLTAIFLIAEITGGYALFVPLIITSTISFLTILYFEPYSIYTKNLAKRGEL
                     ITHHKDKAVLTMLSVRKVLETDFTPVEANQTLGDLVKIVGQSKRNIFPVVQDDGQLVG
                     VVTLDDIRSKMFDTSAYTVVHVQDIMTLAPAVIDVDEPMEKVMQKFEDTGAWNLPVTD
                     GGRYIGFVSKSKIFSIYRKMLVKVSDE"
     gene            2324..2722
                     /locus_tag="PK502_06110"
     CDS             2324..2722
                     /locus_tag="PK502_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPH00423.1"
                     /translation="MMIPHDSKTPLLQGKKILIIEDDTVSRIFLSELLQSTHAEVDFV
                     RTAYEAKKYLDTHQAPNAILLDLRLPDMDGFELAAAIHRQHGDVCIIAQTAYVVEGID
                     EKCRQSGIDAHIYKPIRRDNLLDLLGQYVK"
     gene            2792..4072
                     /gene="glyA"
                     /locus_tag="PK502_06115"
     CDS             2792..4072
                     /gene="glyA"
                     /locus_tag="PK502_06115"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPH00424.1"
                     /translation="MVRDTKIFDLIEKERQRQMHGIELIASENFVSPQVLEAMGSVMT
                     NKYAEGYPGARYYGGCEMVDQSEQLAIDRLKELFEAEYVNVQPHSGAQANMAVFVAVM
                     KPGDTFLGLDLSHGGHLSHGSPVNFSGMWYKAVSYGVKEETGRVDYDMMERVALEQKP
                     RLIVGGASAYSREWDYKRMRAIADAVGALLMIDMAHPAGLIAAGLLDNPLRYAHIVTS
                     TTHKTLRGPRGGIILMGKDFPNPWGLTTPKGEVKMMSQILNSSVFPGIQGGPLEHVIA
                     AKAVAFGEALRPEFKEYQRQVKLNAATMANAFMDKGYKVVSGGTDNHSMLIDLRTKFP
                     EITGKKVENALVRADITINKNMVPFDSRSPFQTSGIRVGTPAITTRGLKEQHMNHIVD
                     LMDRVISSADDEKTITAVKEEVNKFMRDFPLYAW"
     gene            4231..4713
                     /locus_tag="PK502_06120"
     CDS             4231..4713
                     /locus_tag="PK502_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00425.1"
                     /translation="MELFHIKLNEFPYRKSKFFRLLIVAAIILALLAILTILIFVNRQ
                     EKGFVGIILIWVSYFLVYLYWAWYSFRVKLYIHADEFAIKFKFGMVPSLSQVILWETI
                     EKVRLGPTYIAFYKKTGRRKVAKLGWLPYSKVVEIKDKVANACRIKHIPVEVADFTHY
                     "
     gene            4737..5486
                     /locus_tag="PK502_06125"
     CDS             4737..5486
                     /locus_tag="PK502_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPH00426.1"
                     /translation="MEWWIYLLVVLAGVAAGFINTLAGSGSLLTLPLLIFIGLPANVA
                     NGTNRIGILMQSMVAVGRFKQKKVFEWSDGLWLTIPAGLGAVLGALVAVDLNEVLMNR
                     IIGILLVFMFFLLLFEPEKWLKPKNPDSRIRFTPFRFIVFFVIGIYGGFIQAGVGFFL
                     LAGLVLGAGMDLVRSNALKVLITMVFTILALVVFIINGQVDFKLGIIMGIGNMIGAWI
                     ATSVALKHGASFIRWVLLICVLAFGLKLLIW"
     gene            complement(5495..6331)
                     /locus_tag="PK502_06130"
     CDS             complement(5495..6331)
                     /locus_tag="PK502_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPH00427.1"
                     /translation="MTITANKAKGIAATVLFHILMVLLLMMFGFNIPFPLPAEEGIII
                     NFGNSDEGMGLEEPAIGEQAISNPAGYPTMQEDESPLTQDYEEAPALPVKKEESKKKV
                     AEPTEKTNPASSTDKVAPSTTAATPEVEKPREANAKALFPGQKINGGQTGGEGVTGKP
                     GNQGSLEGSASSTNRTGGATGGGGDGQNGIITNLNGRSALLLPKPDYPKQKEGRVVVE
                     VTVDNHGNVTKAIAGIKGSTTLDTDLLKAAEKAALSAKFDVSSNAPSFQVGTITYIFR
                     LN"
     gene            complement(6335..6739)
                     /locus_tag="PK502_06135"
     CDS             complement(6335..6739)
                     /locus_tag="PK502_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPH00428.1"
                     /translation="MALKHRSKSDPGLNMSSMTDIVFLLLLFFMISSTLVHPNALRLL
                     LPQSNSQISDKPVTSVSITADRNFYLETVPVSIGQLEYLLQQKMKGENDPTIALHVDR
                     SVPMEDVVKVMNIAKDNGYKLVLATQPIKKEQ"
     gene            complement(6751..7392)
                     /locus_tag="PK502_06140"
     CDS             complement(6751..7392)
                     /locus_tag="PK502_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPH00429.1"
                     /translation="MGQSGEAKMRFFDMAVKGGWIMIPIFILSIIAVYIFVERYMAIH
                     KASKIDANFMNKIREYIHEDKVEAALALCQSQNTPVARMIEKGIQRIGRPLSDVNSAI
                     ETAGNLEISKLEESMPLLATIAGGAPMLGFLGTVIGMIRAFFDMSMAGNSIEIGLLSN
                     GIYIAMVTTVAGLIVGIPAYFGYHYLVARIEKVVYTMEANISEFMDLLNEPVA"
     gene            complement(7547..12073)
                     /locus_tag="PK502_06145"
     CDS             complement(7547..12073)
                     /locus_tag="PK502_06145"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPH00430.1"
                     /translation="MLRVVIVLLAVPSLIWLTLQNSRIQTYLVDQVTASLKERLGIEV
                     SVGRVDFRPFASLLLRDVFIADLKGDTLLHSGRIAVTLLGLKLAPSNIELGRANFDQT
                     TFNFVTDSSGVMNLSGLIRKIKETFPSKKDSSDFRLSANSISISNSRFILKKTAPKAR
                     NSDFGMNFQDMNFSNFNLKADNLYIHRDTVALSINSLNFSDHSGFKLDNLRANFSLCR
                     SNMKFDKLRLQAHDSNIRLNRLNFAFSSWKDFGNFVHSVELDAQFAGTYLKTSTLSYF
                     APVFRKVDLAVSVDGHVKGTISDLRGKGLELRYGSQTLLRTNFNITGLPDIKQTLMVI
                     DLYELSTNREDLAAITNPSSGKPLVNLPQNFDMLSTLTYKGNFTGYLNNFVAYGNLTS
                     AIGSIAVDLSFVPDKSNNIDINGNLRTTQLDVGKFSNSPILGKATLSASLNGTIDNQR
                     KINFFTNATINSLQANSYTYNDIVISGNLTNKTYSGSVRLNDPNCKLNFMGIIDFSDT
                     IPVFDFSAFVPKLDLVKLNLNKKDSVSQASFLLTTKFRGSNLDNSSGEIKVVNSMYRN
                     QHGEFKLDDVTINADNKEDSKVITLQSDFAEGELRSKYNYSKFFTYLNNLVLKYIPAM
                     KPESETESSTVAFTGVEKPEYNDYLVKFRLKKTEKITSVLMPQLSIAENTSIFGILNP
                     DLQTFSFKVRVPQMRFGNTGIKDLAAEGQTKDSLFTASINTPLITIGSSKIENFKISS
                     YLSHNLLNLAIGWQNTQQPASRGLIEATADFTSYNKNGQLTSIAFNPSTFVINDSLWN
                     LSPSTVVIDTASVKFNSFSIRSRNQHFNIDGKLSASNMDTLKVNLLNMDISYLNLYLQ
                     NRGYNVSGTINGFAEINSAYQNPTLFSKLSINKLILNSNPIGNVTFSSTWINSEKRMA
                     VELTNNHEDTLTLKATGNLYTDDGSMDFLVDVNRVKLVHLAPLLKGSVSDLSGNVNGK
                     IRVNGKTSKPMLNGNLSFSNATLTVDFLKSTFTINDKISVDNSNLYFRNFKITDRFNR
                     VATINGDIKTNSFKDINFNIGMAAENFLCMNTTEHDNSQFYGTVFGTGPIFITGKPDN
                     LNFNINLKTEGKTAIYLPISTEKTVKQNNFISFVSSNPDQIVIDEPVATKKSKVNLNL
                     NLDLLVTPDAEVQIIIDKKLGDIIKANGSGNLKMEISPEKDVFRMFGNFDIEKGDYLF
                     TLQGVINKKFTIMPGSSITWNGGPLDATMNIEAAYRVKTSLKQLLMDERYTTRVPVDC
                     KIFLTQKLTSPVIKFGIEVPNADAETKALVDGALNTEEKVNTQFLGLLVINSFIADPN
                     QAGMMTQSSSSMGTMGLYNTASELLSNQFSNWISQWSNSVDIGVNYRPGMESELSSNQ
                     MEVALSTQLLNDRVSINGNVDVGRRSTSSPIAGDFNIDVKLNQSGKLRLKAFARTNDD
                     LLPTNQQNSYTTGVGVLYREDFNNMKDLRRRIRNTFNSEESFSPIIVPENSSQPDSTK
                     DKTSSYPSPFVQFR"
     gene            12235..13254
                     /gene="tsaD"
                     /locus_tag="PK502_06150"
     CDS             12235..13254
                     /gene="tsaD"
                     /locus_tag="PK502_06150"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPH00431.1"
                     /translation="MSITILGIESSCDDTSAAVIRDGFLLSNLISSQEVHREYGGVVP
                     ELASRAHQANIIPVVDGALKKAGVGIGEINAVAFTQGPGLMGSLLVGSSFAKGVALSR
                     QIPLVAVNHLQGHVLAHFISDDKAQDHPSFPFLCLLVSGGHTQLIIVRKPLEMEIVGQ
                     TIDDAAGEAFDKCAKVMGLPYPGGPVVDKLAAGGNIHAFRFARPVIKGLDFSFSGLKT
                     SFLYTLRDRVAGDPDFIAKNKADLCASLQHTIIEILMAKVEAAAKKFGVTQVAIAGGV
                     SANSGLRKAITELGDKRGWQVFLPQLRYTTDNAAMIAITGYYKYLAGEFSPLDVMPMA
                     RIPLL"
BASE COUNT         3297 a         2870 c         3382 g         4075 t
ORIGIN      
        1 cgccggtagg cggacaagtt ccgtcaggtt accgacaagt tccgccggta gtcgagcaag
       61 ttccgaaatt aggaatccgc tgttaggcgg acgagttctg aaacccacct ccttgtatcc
      121 tttactattt ttgattataa ccccaggaaa tcggccaggc aggtcctgca ggcttaatcc
      181 agttaacatc atgcccggga accaattgct gaaaataatg gctacatttg ctattggtag
      241 caaaatagtt tattcaaaaa tcttttagat gagaccttca ggattgaaaa ggcaatttag
      301 caggacgtgg catggattca gaaggcttgg ggaaagaaga ataatgctga ttcttagcgt
      361 agtggttggc gtttcaagcg ggctggccgc agttcttctt aaaaatgtgc ttcatgttgc
      421 taatgttttt gttcaggaat cgttccctaa ggattcagcc aacttcttat tttttgctct
      481 accggcaaca ggtatcctgc taaccattgt tttcgtgcgt tttttggtga aggacgacat
      541 tgggcatggc gtttcaaagg tgctttactc aatctccaag caaaacggac gaataaagcc
      601 tcataacatg tacagctcaa tggtggccag cacctctact attgcctttg gcgggtcggt
      661 tggcgccgaa gccccaatag tttacacagg ggcagccata ggatcaaacg ttgggaattt
      721 tttcaggtta aactacaaga atattatctt gcttattggc tgcggcgccg ctggagcaat
      781 tgcatcaatt tttaaggcgc cccttgcagg aatagtattc acgctagagg tgctcatgct
      841 cgatttaaca actgcctcta tagttcccct gctcatttcg gcagtgtcag ctgctacggt
      901 ttcctacttt tttttgggtc gggaggtagt tttagcctat gatgttgtac gtcccttcgc
      961 gcttcagaag ataccctact acgtgctgct gggggtgttt tgcggtttca tatcgctcta
     1021 cttcatcagg gtggtaatgt ggattgagaa acgcttcgaa aggattacca gcatataccg
     1081 gcgctggatt ttaggggcta tgattttggg tacttttacc tttttgttcc ctccgcttta
     1141 cggcgagggt tacgaggtgc tgcaggctct actcgatgga cgttcatcgt tcatattcaa
     1201 tagcagcccg ttctatgaat ttcaaagtaa cgtttggttg ctgctggtgg cgcttctcat
     1261 tttaatttta gccaaaggtg tggcaacagc cgttactact ggtgctggtg gcgttggggg
     1321 aacttttgcc ccaacactct ttataggagg ggtggcaggt ttttttgtgg ctaaactctt
     1381 aaaccagctt ggtataactg ttgtgtcgga aagtaacttt gcactgattg gaatggctgg
     1441 cgccatggcc ggggtgatgc acgcacccct aacggccatc ttcctgattg ctgagattac
     1501 tggcgggtac gctctttttg tcccgctaat catcacctct accatttcgt ttctaaccat
     1561 tctttacttc gagccctact ccatctacac aaaaaacctt gccaaaaggg gtgagcttat
     1621 cactcatcat aaggataagg ctgtgctcac tatgctgagc gttcgcaagg tgctcgaaac
     1681 agactttaca cctgttgaag ccaatcaaac ccttggcgac ctggttaaaa ttgttggcca
     1741 atcgaagcgc aacatctttc ctgttgtgca ggatgatggc cagttggtgg gggttgttac
     1801 ccttgacgat atccgctcta aaatgttcga tacttcggcc tataccgttg ttcatgtaca
     1861 ggatataatg actcttgccc cggccgtcat tgatgttgat gaaccgatgg agaaggtgat
     1921 gcaaaaattc gaggataccg gcgcatggaa tctcccggtt actgatggtg gaagatacat
     1981 tgggtttgtt tcgaaatcaa aaatattctc catttaccgc aaaatgctgg tgaaagtttc
     2041 cgatgagtaa acatgtattg tgtaatgttg tttaaaaggt ttgaaccaat caattttttg
     2101 tagtaattta tcttttttat gtttttgaca cgctttgttt tggcgatttc tttttgatgg
     2161 gggtttcagg gtttactctt cggcgattat tgtgctaaat gccaacacgt tgagatttga
     2221 aacaaacatg tagggcagct tgtatgaacg cgtatgaaca accgggtaat atttgcgggt
     2281 tcggctgtga aagggaatta atgagaaacc taaaaatttt gtaatgatga taccacacga
     2341 ttcaaaaacc ccactccttc aagggaaaaa gattttgata atcgaggatg acacggttag
     2401 ccgcattttt ttgagcgagc tcctacagtc aacccatgcc gaggttgact ttgtacgtac
     2461 ggcatacgaa gccaaaaagt atttagacac ccatcaagcg ccaaatgcca tactgctgga
     2521 tttgcgccta cccgacatgg atgggtttga gctggcagcg gctatccacc ggcagcatgg
     2581 cgatgtgtgc ataattgcac aaaccgccta tgtggttgag gggatagacg agaagtgccg
     2641 gcagtcggga attgatgccc atatctacaa gcctatcagg cgggataacc tgctggattt
     2701 actcggacaa tatgttaagt aatatgcagg taagctgatt agcgacagcg ataaaagact
     2761 acatttgcca taaaataata aatcattcag catggtacgc gatacaaaaa ttttcgacct
     2821 cattgaaaag gagcgccaac ggcagatgca tggcatcgag ttaattgcct ccgagaattt
     2881 tgtaagccct caggtgctag aagccatggg ctcagtgatg accaacaagt atgccgaggg
     2941 ctatccgggc gcaaggtact acggtggatg cgagatggtt gaccaatcag aacagctggc
     3001 catcgaccgg ctgaaggagc tttttgaggc ggagtacgtt aatgttcagc cccactcggg
     3061 tgctcaggcc aacatggccg tatttgttgc ggttatgaaa ccgggcgaca cctttcttgg
     3121 cctcgacctt tcgcatggcg gccacctgtc gcatggttca ccggtaaatt tctcggggat
     3181 gtggtacaaa gcagtttcct atggtgtaaa ggaggaaacc ggacgcgttg actacgatat
     3241 gatggagcgg gtggctcttg agcaaaaacc aaggttgatt gttggaggcg cttcggccta
     3301 ctcccgtgaa tgggactaca agcggatgag ggcaatagcc gatgcggtag gagcattact
     3361 catgatagac atggctcacc ctgcaggcct gattgccgca ggattgctcg ataatcccct
     3421 tcgttacgcc cacattgtta cctcaaccac ccacaaaacc cttcggggcc cacgtggcgg
     3481 aattatcctg atgggtaagg attttcctaa tccatggggg cttactacgc caaaaggcga
     3541 ggtgaaaatg atgtcccaaa tactcaactc cagcgttttc cccggcattc agggtggacc
     3601 gctggaacat gttattgccg ctaaggctgt agctttcggt gaggccctta gacctgagtt
     3661 caaggaatac cagcgtcagg ttaaacttaa tgctgcgaca atggccaatg cctttatgga
     3721 caagggatac aaggtggttt cgggtggcac cgataaccac tccatgctta ttgacctgcg
     3781 tactaagttc cccgaaatta ctggcaaaaa ggtggagaat gcccttgttc gagccgacat
     3841 caccattaac aagaatatgg tgcctttcga cagccgttcg ccattccaaa cttccggtat
     3901 cagggtcggt acacctgcaa ttactacccg tgggctgaag gagcagcaca tgaaccatat
     3961 tgtcgatttg atggataggg ttatctcaag tgctgatgat gaaaaaacaa ttactgccgt
     4021 taaggaggag gttaacaagt ttatgaggga ctttccactt tatgcatggt aggcttgttt
     4081 tacgtatttt gcactcttgt tagtatgaac ctggatttaa tgcacgtggt gtataaaagt
     4141 ggaaccgagc ctgcaggttt ggttccatta ttataacggc gattagctta ccaagctaat
     4201 acatgtctac cactcctagc taacaaaaag atggaattgt tccacattaa gctaaacgag
     4261 tttccctaca ggaaatcgaa atttttcagg ttgctaattg tggcggctat tattttagcc
     4321 cttctcgcta ttttaaccat tttgattttt gttaatcggc aagaaaaggg atttgtaggc
     4381 ataatcttaa tctgggtttc ctatttcttg gtttacctct actgggcatg gtattcgttc
     4441 agggtaaagc tctacatcca tgccgatgaa tttgccatta agttcaagtt tggcatggtt
     4501 cccagcttgt cgcaggtaat tttatgggaa accattgaga aggttaggtt gggccctacc
     4561 tatattgcct tttacaagaa gaccggtagg cgaaaagtgg ctaagttagg ctggctgccc
     4621 tactctaagg tggttgaaat taaggacaag gttgccaacg catgcagaat aaagcacatt
     4681 cccgttgagg tggccgattt tactcattac tgatactttt gatttgttta tttgatatgg
     4741 agtggtggat atacctatta gttgttctgg caggagttgc agcaggtttt atcaacacac
     4801 ttgccggtag tggttcgcta cttaccttac ctttattgat tttcattggt ttacctgcaa
     4861 atgtagccaa cggaaccaac cgtattggaa ttcttatgca gagcatggtt gcggttggta
     4921 ggtttaagca aaaaaaggtt tttgagtggt ccgatggtct ttggctgact attccggcag
     4981 gtctgggcgc tgtgctgggc gcactggttg ccgttgacct taatgaggtt ctaatgaacc
     5041 gaattatagg cattctgcta gtattcatgt tcttccttct gctttttgaa cccgaaaaat
     5101 ggcttaaacc taaaaatccc gatagtcgta ttcgctttac tccgtttcgg ttcattgtct
     5161 tttttgtaat aggaatctac ggaggattta ttcaggctgg cgttgggttc ttccttttgg
     5221 cagggctggt gctgggtgca ggtatggatt tagttagatc gaatgctctt aaggttttga
     5281 taactatggt attcaccatt ttggcgttgg tggtgtttat cattaacgga caggttgact
     5341 ttaagctggg catcatcatg ggcattggca acatgattgg tgcctggatt gctaccagcg
     5401 ttgcgctaaa acatggtgca tcatttattc ggtgggtgtt gctgatttgt gtgctggcct
     5461 ttggcctcaa gctgttaatc tggtagcctt tgtgctagtt tagcctaaag atataggtaa
     5521 tagttccaac ctggaaggat ggggcgtttg acgatacgtc gaatttggca ctcaacgccg
     5581 ctttctcggc agcctttagt agatcggtgt caagagtggt tgaacccttt ataccagcaa
     5641 tagcttttgt aacatttcca tggttatcaa cagttacttc caccactaca cgaccctcct
     5701 tctgcttggg gtaatcgggt ttgggaagaa gcagcgcact tctcccatta aggttggtga
     5761 taattccatt ctgtccgtca ccgcctccac cggttgctcc gccggtgcgg ttggttgagc
     5821 tggcagaacc ctcaagcgat ccctggttgc ccggcttgcc tgtaactccc tctcctccgg
     5881 tttgcccgcc attaattttt tgtcctggga aaagcgcctt ggcatttgcc tcccgtggct
     5941 tttcgacttc gggagttgct gccgttgttg atggagccac tttgtccgtg ctactggcgg
     6001 ggttggtttt ttccgttggt tcagctactt tctttttgct ctcctccttt ttaaccggta
     6061 aggctggggc ttcctcgtag tcctgtgtta ggggactttc atcctcctgc atggtggggt
     6121 atcccgccgg gtttgaaatt gcctgctcgc caattgctgg ttcctccaat cccatacctt
     6181 cgtcggagtt gccaaagtta ataataatcc cctcttcggc tggtaggggg aagggtatgt
     6241 taaagccaaa catcattaaa aggagaacca taaggatatg gaacagaacg gttgctgcta
     6301 ttccctttgc cttattggcc gtgattgtca tttgttactg ctcctttttt attggttggg
     6361 tagctaaaac cagcttgtat ccattatcct tggcaatatt catcacttta accacatctt
     6421 ccatcggaac cgacctgtca acatgtagtg ctatggtagg gtcattttct cccttcattt
     6481 tctgctgaag aaggtactcc agctggccaa tggataccgg aacagtttcg aggtaaaagt
     6541 tgcggtcggc tgtaatggaa accgaggtaa cgggtttgtc ggaaatctgg ctgttgcttt
     6601 gtggaagtag cagcctcagg gcgttggggt gtactagggt agaggagatc atgaagaata
     6661 gcagcagcag gaacacgatg tcggtcatgg acgacatgtt aaggcctgga tcggattttg
     6721 aacggtgttt taatgccatg atgtaacgtt ttatgctaca ggttcgttaa gcagatccat
     6781 aaactccgaa atatttgcct ccatggtgta aaccaccttc tcgatccgtg caaccaggta
     6841 atggtagccg aagtaagcag gaatccctac aattaatccg gcaacggtgg taaccatggc
     6901 aatgtatata ccgttagata aaaggcctat ttcgatactg ttgcccgcca tggacatgtc
     6961 gaagaacgca cgaatcatac caataactgt gccaagaaac cctagcattg gggcaccacc
     7021 ggcaatggtg gcaagcaatg gcatgctctc ttcaagtttg gaaatctcca ggttaccggc
     7081 agtttcaatt gccgagttta catccgataa gggcctgcct atgcgttgaa ttcctttttc
     7141 aatcattcgg gcaactggtg tgttttgcga ttggcaaagg gctagtgccg cttcaacttt
     7201 atcctcatga atgtactccc ttatcttgtt cataaagttg gcgtcaattt tagaagcttt
     7261 atgaatggcc atgtagcgtt caacaaaaat ataaacggca ataatggaga ggataaaaat
     7321 gggaatcata atccaaccgc ccttaacggc catatcaaaa aatctcattt tagcctcgcc
     7381 gctttgtccc atacccgttt gggcctgagc tgccgataga agaatagtgg tgagtgtagt
     7441 cattgcttga aatttaattt tgcgaaaatt actaaaaaaa cgtttaaaat gaccagggta
     7501 ttgtttaggc taaattaata acccacaaat aatggggttg ggcgtgctac ctgaactgga
     7561 caaatggaga agggtaggag cttgttttgt cttttgtact gtcgggctgg ctgctatttt
     7621 ctggaacaat aatggggctg aagctctctt cggagttgaa ggtgttgcgt attcgccgcc
     7681 ttaaatcctt catattgttg aaatcttcac ggtagagaac tccaaccccg gtagtgtagc
     7741 tattctgttg attggtggga agcaagtcgt cgtttgtacg ggcaaaggct ttcagtcgca
     7801 gcttacccga ctggtttagc ttaacgtcaa tgttgaaatc acctgcaatt gggctgcttg
     7861 tgcttcgcct acccacatct acattaccgt tgatggatac cctatcgttg agcagctggg
     7921 tagatagtgc cacctccatc tggttggagc tcagctcact ttccatgccg ggtctgtagt
     7981 taacacctat gtcaaccgaa ttgctccatt gcgatatcca gttgctgaac tggttggata
     8041 gtagctcgct ggcagtgttg tacaggccca tggtacccat ggagcttgag ctctgggtca
     8101 tcattccggc ctggttggga tcagctataa aactgttgat taccagcaga cccaggaact
     8161 gggtattaac cttttcctcg gtgtttagcg ctccatcaac cagggctttt gtttcggcat
     8221 cggcgttggg aacttcaatt ccaaacttga ttacgggcga agtgagcttc tgggtgagaa
     8281 aaattttaca gtcaaccggt acgcgggtgg tgtacctttc gtccatcagt agctgcttga
     8341 gcgaggtttt tacccggtac gcagcttcaa tgttcatggt ggcatctaga ggtcctccgt
     8401 tccacgttat gctgctgccg ggcattatgg taaatttctt gttgattacc ccttggaggg
     8461 taaaaaggta gtcccccttt tcgatgtcaa agtttccgaa catcctgaaa acatcctttt
     8521 ctgggctaat ttccattttc agatttccgc taccgttggc cttgatgatg tcgccgagct
     8581 tcttgtcaat aataatctgc acctctgcat cgggggttac cagcaggtcg aggttcaggt
     8641 tgaggtttac ctttgacttt tttgtcgcta caggttcgtc tataactatt tggtcgggat
     8701 tgctgctaac aaaggaaatg aagttgttct gctttacagt tttctccgtt gaaatcggga
     8761 ggtagatggc tgttttgccc tctgttttta ggttgatgtt gaagtttagg ttatcgggtt
     8821 taccggttat aaagattggt ccggtgccaa acactgttcc ataaaactgg ctgttgtcgt
     8881 gctcggtagt gttcatgcag aggaaattct cggcagccat gccgatgtta aagttgatat
     8941 ccttaaagct atttgtcttg atatcaccgt ttatggttgc tacccggtta aaccgatcgg
     9001 tgattttgaa atttctgaag tataggttgc tgttgtcaac ggaaattttg tcgtttatag
     9061 taaaggttga ctttaagaaa tcaacagtta gcgtggcgtt gctaaatgag aggtttccgt
     9121 taagcattgg cttgcttgtt ttcccgttca ccctgatttt cccgtttaca tttccggata
     9181 ggtctgaaac gctacccttt agcaaggggg ctaggtgaac cagcttgaca cggtttacat
     9241 ctaccagaaa atccatactt ccatcgtcgg tgtagaggtt tccggttgcc ttcagggtta
     9301 gagtgtcctc atggttgttg gttagctcaa ctgccatgcg cttttctgaa ttaatccagg
     9361 tgctggagaa ggttacattt ccaatgggat ttgagtttag gattagcttg ttgatgctta
     9421 gtttggaaaa aagcgttgga ttttggtatg cactgttgat ctcggcaaac ccgttaatgg
     9481 ttccgcttac gttgtaccct ctattctgca ggtaaaggtt aaggtatgat atgtccatat
     9541 taaggaggtt taccttgagg gtatccatgt tactggctga aagtttacca tctatgttga
     9601 aatgctgatt tctgctgcga atggagaagc tgttgaattt tactgaggcc gtatcaataa
     9661 ctactgtcga gggggacagg ttccataatg agtcgtttat cacaaatgta gatgggttaa
     9721 aagcaatgct ggtgagctga ccattcttgt tataacttgt aaaatcggca gtggcttcaa
     9781 ttagcccgcg ggaggcgggt tgctgggtgt tttgccagcc tatggcaaga ttcagcaggt
     9841 tatggctaag gtacgagctg atcttgaaat tctcgatttt tgaactacca atagtaatca
     9901 acggtgtatt gatgctggcc gtaaataggc tgtccttggt ttgcccttca gcagctaaat
     9961 cttttatgcc ggtatttcca aaacgcatct ggggtaccct gactttaaaa gaaaaggttt
    10021 gcaagtcggg gttgagaata ccgaatatgc tggtgttttc ggcaatgctt agctgaggca
    10081 tgagaacgct ggtaattttt tcggtttttt taagcctgaa tttaacaagg tagtcgttgt
    10141 attcaggttt ttcaactcct gtaaaggcta cagtggagga ttctgtttca ctctccggct
    10201 tcattgccgg tatatacttc agaacgaggt tgtttaggta ggtaaagaat tttgagtagt
    10261 tatacttact ccgtagttct ccctcagcaa agtccgactg aagggtaata accttgctgt
    10321 cctctttatt gtcggcattt atggttacat cgtctagttt aaattcccca tgttggtttc
    10381 ggtacatcga gttcacaacc ttaatttccc cgctgctgtt gtcgaggttg ctgcccctga
    10441 acttggtggt aagcaagaat gaggcttggg aaaccgaatc ctttttgttg aggttgagct
    10501 ttaccaggtc cagctttggt acaaaggctg aaaagtcgaa tacgggaatc gtgtcggaaa
    10561 agtcaataat ccccataaag ttgagcttgc agttgggatc gttgagacga accgatccgg
    10621 aataggtttt gtttgtaagg ttacccgata ttacaatgtc gttataggtg tagctgtttg
    10681 cctgtaggga gttgatggtt gcgttggtga aaaagttaat ttttctctgg ttgtcaatgg
    10741 tcccgttgag cgatgcgctt agtgttgctt ttccaagaat tgggctgttg ctaaactttc
    10801 ccacatccag ctgtgtagtt ctgagattcc cgttgatgtc tatgttgttg cttttatcag
    10861 gcacgaatga taggtcaacg gcaatactgc ctatggcgct ggttagattt ccgtaggcaa
    10921 caaagttgtt gagatagccg gtaaagttac ccttataggt tagggtgctg agcatgtcaa
    10981 aattctgggg aaggtttacc aggggtttgc cggaagaagg gttggtaatt gcagctaaat
    11041 cctcacggtt ggttgataat tcatatagat caataaccat aagggtttgt ttaatgtccg
    11101 ggagcccggt tatgttaaag ttggttcgga gtaatgtttg cgatccgtac ctcagctcaa
    11161 gtcccttacc gcgtaagtct gaaattgtac cctttacatg gccatcaaca ctaactgcta
    11221 aatcaacctt gcgaaacacg ggtgcaaagt aggagagggt gctggtttta aggtatgttc
    11281 ccgcaaattg tgcatccagc tccacgctat ggacaaaatt gccaaaatcc ttccagctgg
    11341 aaaaagcaaa attcagcctg ttgagcctta tgttggagtc gtgtgcctgc aacctcaact
    11401 tgtcaaattt catgttcgac ctgcataagc taaaattagc ccggaggtta tcgagcttaa
    11461 atccggaatg gtcgctaaaa ttaaggctgt ttattgaaag tgctacggta tcacgatgaa
    11521 tgtataggtt gtcagccttc aggttgaagt tgctgaagtt catatcctga aagttcatgc
    11581 cgaaatccga atttctggct tttggagctg tttttttcag tataaacctg gagttgctaa
    11641 tagaaatact gttagcgctg agcctgaaat ccgatgaatc tttttttgag ggaaaggttt
    11701 cttttatctt tctaattaac ccgctaaggt tcataacgcc cgacgaatcg gtaacaaagt
    11761 tgaaagtggt ctgatcgaag tttgcccttc ccagctcgat gttgctgggt gccagtttaa
    11821 ggccaagcag ggtaacggct atcctgccag aatgtaggag tgtgtcgccc tttaggtcgg
    11881 caataaaaac atcccttaga agcaagctgg caaatgggcg aaaatcaacc ctgcctaccg
    11941 atacctcaat tcctaagcgc tctttaaggg aggcggttac ctggtcaacc agataggttt
    12001 ggatccggct gttctgaagg gttaaccaga ttaatgaggg tacggccagc aggacaatga
    12061 ctacccttaa caatattttt gctgtttttt tgataccttt gcctatattt atgggtatag
    12121 taaacctaaa tttaattctc aaacttcgtt ccgaaagtaa gcaattagaa atgttttttg
    12181 gaattgtcca ttccgaatgt ttcaatcaat ttgatggttt aataaaaatt aacgatgagc
    12241 ataactattc tggggattga atcgtcatgc gatgacacct ctgcagcggt gattcgcgat
    12301 ggattcttgc tctccaacct aataagcagc caggaggtgc atagggagta tggaggcgtt
    12361 gttccggaac ttgcctcgag agctcaccag gcgaatataa ttccagtagt tgacggagcg
    12421 ctaaaaaaag ccggtgtggg tattggcgaa atcaatgcag ttgcctttac acagggtccc
    12481 ggcttaatgg gttcgctcct ggttgggagt tcgtttgcca agggagtagc cttgtcgcgt
    12541 caaattcccc ttgttgcagt taaccatttg caggggcatg tgcttgccca cttcatcagc
    12601 gacgacaaag ctcaggatca cccatcgttc ccattcctat gtctgctggt ttcgggtggg
    12661 catactcagc ttattattgt tcgtaagccc cttgagatgg agattgttgg gcaaactatt
    12721 gatgatgctg caggcgaagc tttcgacaaa tgtgccaagg tgatggggtt accctacccg
    12781 ggagggcctg tggttgataa gctggctgcc ggggggaaca ttcatgcttt tagatttgcc
    12841 cgtcccgtaa ttaaaggact tgacttcagc tttagtggac ttaaaacgtc gtttttatat
    12901 accctgcgcg atagggttgc tggcgaccct gattttattg caaaaaacaa ggccgattta
    12961 tgcgcatcgc ttcaacatac cattattgag attcttatgg ccaaggtgga ggctgccgcc
    13021 aaaaaatttg gagtaacgca ggtggccata gctggaggtg tatcggcaaa ctccggcctg
    13081 cgaaaggcca taaccgagct aggcgataaa cgtggctggc aggttttcct gccccagctc
    13141 cgttatacta ccgataacgc cgctatgatt gctattaccg gctactataa gtatctggca
    13201 ggagaatttt cacctcttga cgttatgcca atggctcgaa tacctttgtt gtaaaaggat
    13261 aaggagtaag tttgaaaggt ttgagtttga ccagcctggg cggaacttgt ccagcctttg
    13321 acggacatgt ccgactttcg gcggaaattt atcgtatttc aggcttgatt tcaggaggaa
    13381 tttaaagtcc cgaagtggct aaatatatgt atatcatggc tttagacctg agtgtttggg
    13441 atgggtaaaa attggcgacg caagcagcag ctgctccaaa gcacaactcc aacactttcc
    13501 atcgcaacgg gaagaaatat ggttaagaat actccgcccc ttcagggctt aacgctgcag
    13561 gaattattct gttcaagggg cttcacccaa cctaattgaa tccgtccacc ttttccgggt
    13621 acac
//
LOCUS       DAOPHX010000064        13553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_5309_length_13553_cov_5.591495, whole genome shotgun sequence.
ACCESSION   DAOPHX010000064 DAOPHX010000000
VERSION     DAOPHX010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 13553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13553
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5309_length_13553_cov_5.591495"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..616)
                     /locus_tag="PK502_06155"
     CDS             complement(272..616)
                     /locus_tag="PK502_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00432.1"
                     /translation="MKRRGKPFKLALLLFLLLHIFNKNSQGNTKIQVDAFRIICLIVS
                     SEMLGVSFSIAAKRLFRAKDSCIGGRECFTDFFACFDKPEVLLANWVELFVIGRKQFY
                     LITATSHYYYTK"
     gene            complement(659..1144)
                     /gene="nuoE"
                     /locus_tag="PK502_06160"
     CDS             complement(659..1144)
                     /gene="nuoE"
                     /locus_tag="PK502_06160"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPH00433.1"
                     /translation="MSHIKVELKPDQVAKIKEICKSFGNNPGELINVLHQTQGTFGYL
                     PAEVQEVVAAELNIPVAKVYGVVTFYSFFTMIPKGKHPISVCTGTACYVRGAEKVLDE
                     MKRILNVKVGETTPDGKFSLNCLRCVGACGLAPVVLIGEKVYGRVSPDGVKDILKEYE
                     D"
     gene            complement(1159..2937)
                     /locus_tag="PK502_06165"
     CDS             complement(1159..2937)
                     /locus_tag="PK502_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPH00434.1"
                     /translation="METVKLTIDSKQVEVPKGTTIYKAAKSMGIDIPVLCYMDLHDLG
                     VENKPAGCRICVVEIAGRRNLAPSCSTVCMEGMEVKTHTTRVINARRTVMELLLSDHP
                     KDCLTCPKSGRCDLQDMSVRLGIREIPGQEFAAMSTYKKDFSPSIIRDVDKCIMCRRC
                     ETMCNQVQTVGALSAVNRGFMAVVAPAFEQDLVKSPCTYCGQCVAVCPTGALTEVDHT
                     AKVIRALADPTKTVMVQTAPAVRAALGEEFGLEPGTLVTGKMVSALRKIGFNYIFDTD
                     FAADLTIMEEGTELLDRLTRHLNGDKSVKLPILTSCCPAWVNFFEHNYTDMLDVPSSA
                     KSPQQMFGAIAKSYFAEKLGKKREDLVVVSVMPCLAKKYECQRDEFKHEGNPDVDLSL
                     STRELANLIKQFNINFMELPDEDFDRPLGESTGAAVIFGATGGVIEAAVRTAYELHTK
                     KALPRVDFTELRGMDGIRTATIDFDGLPIKIGIAHGLGNARKLLDQVRSGQADFHAIE
                     IMACPGGCIGGGGQPLHHGDSSIIKARMAAIYREDAGKSIRKSHENPYIIQLYEEFLG
                     KPMSEKSHHLLHTQYFDKGKKDIYIK"
     gene            complement(2949..4742)
                     /locus_tag="PK502_06170"
     CDS             complement(2949..4742)
                     /locus_tag="PK502_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPH00435.1"
                     /translation="MAKYKMHLLVCGGTGCRASSSDLIVDRLKAEIASNGLENDVQVI
                     MTGCFGFCEKGPIVKVLPDNTFYTQVKPEDAAEIVREHALKGRKVTRLLYTDPVKKEH
                     VSDSKHMGFYRKQIRIALRNCGFINPENIDEYIARDGYQALGKVLTEMDPQKTIDIIK
                     KSGLRGRGGGGFPTGLKWEIASKNKADQKYVVCNADEGDPGAFMDRSVLEGDPHSVLE
                     AMAICGYSIGASKGLIYIRAEYPLAIKRLKVAIDQAHEYGLLGNDIMGTGFNFDIELR
                     YGAGAFVCGEETALIHSMEGLRGEPTNKPPFPAESGYLGKPTNVNNVETFANVAPIIL
                     KGAEWYTSIGTEKSKGTKVFALAGKINNVGLIEVPMGTTLREIIFEIGGGIRNGKKFK
                     AVQTGGPSGGCLTEKHLDIPIDFDNLVAAGSMMGSGGMIVMDEDDCMVSVAKFYLDFT
                     VDESCGKCTPCRVGNKRLSELLGKITDGNGELEDLERLRNLSNVIKDTALCGLGQTSP
                     NPVLSTLDNFYDEYLSHVVDKKCKAGRCKSLMQYVIDPEACVGCTACARNCPVNAISG
                     ERKKPHEINTAICIKCGACIEKCKFDAISIK"
     gene            complement(4764..5165)
                     /locus_tag="PK502_06175"
     CDS             complement(4764..5165)
                     /locus_tag="PK502_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPH00436.1"
                     /translation="MTKVKSLADLKMMKDSLKSKISLREKGENSENLVQIKVAMATCG
                     IASGAKVVMDFLLEELDKRGINSVVTQTGCMGYCYAEPTIEVKRPGQEPVVFGYVDTK
                     KADEIIEKYIKNGELVDGIIPTNYETIDGEK"
     gene            complement(5280..5828)
                     /locus_tag="PK502_06180"
     CDS             complement(5280..5828)
                     /locus_tag="PK502_06180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPH00437.1"
                     /translation="MRDLSLHLLDIVQNSIAAQATKIDVHLEINPKLDYLALIISDNG
                     KGMNAEFLQQITSPFTTTRTTRKVGLGLPLLMQNAERTGGYLEITSSVGKGTTLRAVF
                     HPNHIDCPPMGDIAGVAAQLICSFPNISFSFSFKTDTSEFSITTLEIAQALEGIPIND
                     TSVFPLVKQVIESSLEELKFPV"
     gene            complement(5874..6614)
                     /locus_tag="PK502_06185"
     CDS             complement(5874..6614)
                     /locus_tag="PK502_06185"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPH00438.1"
                     /translation="MNLYQADLHIHSVLSPCADLEMSPGNIVSMAKNMGLSIIAITDH
                     NSTLHAPLTRRLAENAGILAIYGAEVTTREEVHCVCLFADEDSRADFQNFLEVHIQKV
                     PNKPDILGYQVVVNEKEEIIEEVEYSLHGALNVGINELEAVVHAMNGIFIPAHIDRPK
                     YSLTSQLGFVPPDLNYDALEVSINSSIESICIHNPSLKRKRFIRSSDAHFIEQVGSVT
                     TTFRMENLNFDGLLKAIKGIDGYDIMLP"
     gene            complement(6611..6952)
                     /locus_tag="PK502_06190"
     CDS             complement(6611..6952)
                     /locus_tag="PK502_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HPH00439.1"
                     /translation="MTVLDIVKSLGLKVYTETVGMDREITGGYTSDLLSDVMGNAQAG
                     TVWITLQTHINIVAVASLKDIAAILLVKGLEPEPNTLQKSLEEGIPILGTSKQAFEIS
                     GMLYQLLYSNR"
     gene            complement(6959..8332)
                     /locus_tag="PK502_06195"
     CDS             complement(6959..8332)
                     /locus_tag="PK502_06195"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPH00440.1"
                     /translation="METSTFHHALKIQSDVCIGCAHCMRVCPTEALRVRGGKALLQSN
                     RCIDCGECYRVCPTGAIIVEQDDFNSIFNYKYRVALVPSVLLAQYPEDVSVDMAIHAL
                     LRIGFTKVVIVEDSVDYLITRINAYIINNPSRKPIISSFCPAIVRLVQVRFPWLVGNI
                     MLVKPPIDLTSSFIRRKLTEEGIPEQEVGIFYITPCAAKIAAVKSPEGEDVSEINGVI
                     NMDLIYNRTLRDIRSGGTKRTVEDGAVNISPKSITWALTGGEALSIEAKGLAIDGISN
                     VIEFLEKMETSEVHGVDFLELRACDESCAGGILTVGNSFLSAERLRKQARYIPESKIG
                     FHTQIKISEYSSVLNGKVGLNRLEPRSIVKLDDSMVKAMNKMERVNRYLCYLPGIDCG
                     VCGTPSCQALAEDIVQGEGSLSQCVFLQKIMEQNKLDPKHSFRIMKKIWGENRFIKDC
                     DKPKHKK"
     gene            complement(8351..8770)
                     /locus_tag="PK502_06200"
     CDS             complement(8351..8770)
                     /locus_tag="PK502_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPH00441.1"
                     /translation="MKFEYKVQGGDFVRAGHASSEVKKILKQLNVEPSLVKRVVVALY
                     EAEVNIVAHAYNGMAYVDIDASRVLVRLDDNGPGISDIELAMQPGFSTASSTVREMGF
                     GAGMGLPNIKSNSDEFSISSEVGKGTHLEFVVYLNSK"
     gene            complement(8776..9120)
                     /locus_tag="PK502_06205"
     CDS             complement(8776..9120)
                     /locus_tag="PK502_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00442.1"
                     /translation="MRIGELVKHIDGKVLCCEECLDSEVVYAFASDLMSDVLTVKCEN
                     FLLITGLSNIQAVRTAEMSDIQVIVFVRNKRVSEDMIRLARENSIVLVECSCTMFKVS
                     GILYGLGIKAVY"
     gene            complement(9547..11337)
                     /locus_tag="PK502_06210"
     CDS             complement(9547..11337)
                     /locus_tag="PK502_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPH00443.1"
                     /translation="MAKQINLTIDGFPITVEEGTTILQAAKKLNIHIPTLCYHDDLCV
                     AGNCRVCVVEMKGSKTLPASCAMPAAEGMEIFTNTIKVRTARKHIIDLLLSEHNAKCT
                     TCYKNGNCELQNLASEYKISEPMFIDLVPYKNYQMDAYSPSIIKDDSKCIRCQRCVRT
                     CEELQHVSALGVAYKGANMKISTFFENPMFEVVCTNCGQCVNRCPTGALVERNYVEEV
                     WDAIYNPNKHVVVQTAPAVRVALGEALGMKPGQIVTGKMVTALRRLGFDSVLDTDFSA
                     DLTIMEEGFELLGRLKKALVDKDSTVKIPMATSCSPGWIKFIEHTYPEYLDNLSTCKS
                     PQQMFGALAKTYYAKKRNIDPASIVSVSIMPCTAKKFEADRPEMHSSGYKDVDYVLTT
                     RELAIMIKQAGIEFDSLPETNYDSIMGDSTGAAVIFGATGGVMEAALRTAYEVVTGRE
                     VPFDGLNITPVRGMEGVREAAIKIENTKPEWKFLDGVVLKTVVAHGLTNAKKVMDVVK
                     SGKADWHFIEIMACPGGCIGGGGQPIPTNEEIRKKRAEAIYREDANKKIRKSHENPEV
                     IAIYKEFLDEPNSHKAHELLHTHYKERQRY"
     gene            complement(11355..12518)
                     /locus_tag="PK502_06215"
     CDS             complement(11355..12518)
                     /locus_tag="PK502_06215"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPH00444.1"
                     /translation="MASNFLRRVDLIFDQDCNFKEVLKSAYQHSNDEIINQLLESGLK
                     GRGGAGFPTALKWKFTSEQADPEKYVVCNADEGEPGTFKDREILFKVPLKVFGGMAIC
                     AKVINAKEGFIYLRGEYRFLLSELENQLKLFNEILKDLKIDFKIKIIVGSGAYICGEE
                     SALFESMEGDRGEPRNKPPYPTVSGFRKKPTVINNVETLVYAFMIFRYGARKFKELGT
                     ADSRGSKVFSVSGDTPKPGIYELELGMTVEQFVDEFGDGDTKAVQVGGASGFCVPRKK
                     FKDTIIGFEGVPTGGSMMLFNSSRSMYNVLKNYLDFFSEESCGQCTPCRVGCQQLILG
                     IEAVKKGDRHAAYLDQLQKLAQTMKITAKCGLGQSVANSFFSIVENFKEEMIY"
     gene            complement(12533..12997)
                     /gene="nuoE"
                     /locus_tag="PK502_06220"
     CDS             complement(12533..12997)
                     /gene="nuoE"
                     /locus_tag="PK502_06220"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPH00445.1"
                     /translation="MTETVELVKKLADKHGRSRESLIPILQGVVQHERYISSEAMTEI
                     ARELDISAAKVYGTATFYSFLDTQPRGKYVIRVCKTISCAMKGKNPILQELEDILKIK
                     VGETTQNRKFSLLETNCLGWCHKGPAMLVNEEAYTELTPEKVREIINDYMKK"
BASE COUNT         3658 a         3274 c         2685 g         3936 t
ORIGIN      
        1 ttcagccttg attttctttg gttctttctt gtatcaagac aagaaagaac acttgctggt
       61 ttttctccac aattttgcct tgcttttcgg cttgccggcc gatgctgttt tttaaaacca
      121 tttttaaagt taggggaatc cgtcaggagc cggacaagtt ccgccagcag gcggacaagt
      181 tcttgaattt tgaatcttga atttttttat cctttatcct tgcttccttc ttctcttcca
      241 tcaaggggaa ataggggtct caatttatcc tctacttcgt gtaataatag tggctggttg
      301 ccgttattag ataaaattgt ttcctgccta tgacaaatag ctctacccaa ttagccaaca
      361 atacctctgg cttatcaaaa catgcaaaga aatcagtaaa gcattccctt ccgccaatac
      421 aggagtcttt tgccctaaaa agtcttttgg ctgcaatgga aaaactcaca cccagcattt
      481 ccgaagatac aattaggcat attatgcgga aagcatcaac ctgaattttg gtgtttccct
      541 gggaattttt attgaatatg tgaagtaaca aaaaaagaag gagtgccaat ttaaatggct
      601 ttccccttct tttcaaccaa cctaaaccta aagctatgaa agataattga aatttgaact
      661 agtcttcgta ctccttaaga atgtccttta ccccatcggg ggaaactctt ccgtaaacct
      721 tttcacctat aaggacaaca ggagctagcc cgcatgcccc aacgcaacgc aggcagttca
      781 acgaaaattt tccatcagga gttgtttcgc ccactttgac gtttagtatg cgcttcattt
      841 cgtccagaac cttttcggct ccacgaacat agcaggctgt gccggtacaa acactaatcg
      901 ggtgcttacc cttgggaatc atggtgaaaa acgagtagaa tgtaactact ccgtaaacct
      961 tggcaaccgg aatatttagc tctgcagcaa caacttcctg aacttccgcc ggaaggtagc
     1021 caaatgtgcc ctgagtttga tggagtacgt tgataagctc accgggatta ttcccaaagg
     1081 atttgcagat ttcctttatt ttagccacct gatcgggctt aagttctact ttaatgtgcg
     1141 acatgggttt cttcctccct atttaatata aatatccttt ttacctttat caaagtattg
     1201 ggtatgaagt agatggtgtg atttctcact cataggttta cccaaaaact cctcgtaaag
     1261 ctgtatgatg tagggatttt catgcgattt tcggattgat ttaccggcat cctccctgta
     1321 aattgcggcc atacgagcct tgataattga tgagtcgcca tggtgtagag gttgtccacc
     1381 tccacctatg cagcctccag ggcatgccat aatttcaatg gcatggaaat cagcctgtcc
     1441 tgaacgaact tggtcaagca gcttgcgggc gttacccagc ccatgcgcaa ttccaatctt
     1501 tatcggaagc ccatcaaaat caattgttgc agtccttatg ccatccatac cgcgaagctc
     1561 ggtaaagtca accctgggaa gtgctttctt ggtatgcagc tcataagcgg tacgaacagc
     1621 agcctcaatt acgccaccgg ttgcgccaaa tatcactgcg gctcccgttg attcgcccag
     1681 tggtcgatcg aaatcctcat cgggcagctc cataaagttg atgttgaact gcttgataag
     1741 gttggccagc tcgcgggttg acagcgatag gtcaacgtca gggttgccct catgcttaaa
     1801 ctcatcacgc tggcattcat acttctttgc caggcagggc atcaccgata caaccaccaa
     1861 atcctcacgt ttcttgccaa gtttttcagc aaagtacgat ttggctatag ctccaaacat
     1921 ttgctggggc gattttgcgc ttgagggaac atcgagcata tcggtgtagt tatgctcaaa
     1981 gaagtttacc catgcggggc agcagctggt aagaatagga agcttaacgc tcttatcgcc
     2041 attgaggtgg cgggttaacc tgtcgagaag ctcggttccc tcctccatta tggtcaaatc
     2101 ggccgcaaaa tcagtatcaa aaatatagtt gaacccaatt tttcgaagtg ccgacaccat
     2161 ttttcccgta acaagagttc ctggctccag gccaaactcc tcgccaagag cagctctaac
     2221 tgcaggggct gtttgaacca taacggtttt ggtgggatcg gcaagcgccc ttattacctt
     2281 ggcagtgtgg tcaacctcgg ttaaagcgcc agtagggcaa acagcaacgc attggccgca
     2341 atatgtacag ggcgatttta ccagatcctg ctcaaaagca ggggccacaa cagccataaa
     2401 acccctattc acggcgctta gtgcaccaac tgtttgcacc tggttgcaca tggtctcgca
     2461 cctacggcac atgatgcact tatcaacatc gcggataatt gagggcgaaa aatctttttt
     2521 gtaggttgac atggcggcaa attcctgtcc gggtatttca cgaataccca acctaacaga
     2581 catatcctga aggtcacacc taccactttt aggacatgta aggcaatcct ttggatgatc
     2641 ggaaagtagc agctccataa ccgtccggcg ggcatttatc acccgcgttg tatgggtctt
     2701 aacctccatg ccctccatgc aaacggttga acagctggga gccagattac gacgaccagc
     2761 aatttcaact acgcaaattc tacaacctgc aggtttattc tcaacaccca ggtcatgaag
     2821 atccatgtag caaagcaccg ggatatcgat tcccatgctt tttgcggctt tgtatatcgt
     2881 agtaccttta ggtacttcaa cctgcttgct atctattgta agttttactg tttccattgt
     2941 tcagcctgtt atttaatgct aatagcatcg aatttacatt tttcaataca tgctccacat
     3001 ttaatgcaga tggcagtgtt aatctcgtgt ggtttttttc tctcaccgct tatggcatta
     3061 acgggacagt tgcgtgcgca ggccgtgcaa ccaacacatg cctcaggatc aataacatac
     3121 tgcataagcg atttgcaccg cccggctttg catttcttat caactacgtg tgaaaggtac
     3181 tcgtcgtaaa aattatcaag ggttgaaagt accgggttgg gtgaagtttg acctaagccg
     3241 caaagcgcag tatccttgat aacatttgaa aggttgcgca aacgctcaag atcttcaagc
     3301 tccccgttgc catcagtaat tttaccaagc aactccgaaa gtcgtttgtt tcctacacga
     3361 catggagtgc attttccgca gctctcatca actgtaaagt caaggtaaaa cttggccaca
     3421 gaaaccatgc agtcgtcctc atccataaca atcatcccgc ccgagcccat cattgagcca
     3481 gctgcaacca ggttgtcaaa gtctatggga atatccaggt gcttttccgt aaggcatccg
     3541 cccgaaggac cgcctgtttg aactgcctta aactttttac cgttccttat gccccctcca
     3601 atttcaaata taatttctcg tagggtagtt cccatgggca cctcaattaa ccctacattg
     3661 ttaattttcc ctgctagcgc aaaaaccttt gttccttttg atttttcagt tccaatcgag
     3721 gtgtaccatt cggctccctt caaaatgatt ggagctacgt tggcaaacgt ttccacgttg
     3781 ttaacgttgg taggcttccc aaggtatccg ctttcagcgg ggaatggtgg tttattggtt
     3841 ggctcgcccc taagaccctc catggaatga ataagagcag tttcctcacc gcaaacaaaa
     3901 gcaccggcac catagcgaag ctcaatatca aaattaaagc ctgttcccat gatgtcgttg
     3961 ccgagcaagc cgtactcatg agcctgatca atggcgacct ttagccgttt aatagccaat
     4021 ggatactccg cccgaatgta aatgagccct tttgaagcac caatactata accgcaaatt
     4081 gccattgctt ctagtactga gtgtgggtcg ccctctagga cagaacgatc cataaaagca
     4141 cctgggtcgc cctcgtcggc gttgcaaacc acgtacttct gatcggcttt gttcttgctg
     4201 gcaatttccc actttaaacc ggtaggaaag ccgccaccac cacgtcccct taatcccgat
     4261 ttcttaatga tatcaattgt tttctgcggg tccatttcgg taagcacttt tccaagcgcc
     4321 tggtagccat cacgggcaat gtactcgtca atgttttcgg gattaataaa accacagttt
     4381 cttagggcaa ttctaatttg cttgcggtag aaacccatgt gttttgagtc ggatacatgt
     4441 tcctttttta ccggatcggt gtagagcagg cgggtaacct tccttccctt cagggcgtgc
     4501 tccctaacaa tttcggcagc atcttcaggt ttaacctggg tgtaaaaggt gttatcagga
     4561 agcaccttga caatagggcc tttctcacaa aacccgaagc aaccagtcat tataacctga
     4621 acatcattct ccaggccgtt ggaagcaatc tcagctttaa gcctgtcaac aatcaaatcg
     4681 ctcgacgaag ccctgcagcc cgttccacca caaaccagca ggtgcatttt atactttgcc
     4741 attacttctg tcttttaaga gggttatttt tcaccatcaa tggtttcgta gttggtgggg
     4801 atgatgccat ccaccagctc gccatttttg atgtactttt caattatttc atcagccttt
     4861 ttagtatcaa cgtacccaaa aaccacgggt tcttggccgg gacgcttaac ctctatggtg
     4921 ggttcagcat agcagtaacc catgcatcca gtttgggtca ctacggagtt tatcccccgc
     4981 ttatccagct cctcgagcaa aaagtccata accacttttg ctccagaagc aatgccacat
     5041 gtggccattg ccaccttaat ctgaacgaga ttttcagaat tttccccctt ttcccgtagt
     5101 gagattttcg acttcaggga gtccttcatc attttcagat cagctaatga ttttactttt
     5161 gtcatagcaa tactccttaa acattcattt ttattgggta tatatcgttt tgaacttttc
     5221 ggtgacaaaa ttacatttta aatgttaaca aactaacaac aaattatatg tttaataacc
     5281 taaacgggaa atttgagctc ctccagggag gattctatca cctgcttaac tagcggaaaa
     5341 acactggtat cattaatagg aatgccctcc agtgcttgag ctatttcaag tgtggtaatg
     5401 ctaaactctg aagtatctgt tttaaaactg aagctaaacg aaatgttagg gaacgaacaa
     5461 ataagctgag cagcaacacc cgctatatcg cccataggag ggcaatctat gtggttagga
     5521 tgaaaaacag ctctaagcgt tgttccctta ccaacagatg aggtaatttc caggtaacca
     5581 cccgtgcgct cagcattttg cattaaaagg ggtaatccta atccaacctt gcgggttgtc
     5641 cgggttgttg taaaagggct cgttatctgt tgaagaaact cggcattcat accttttcca
     5701 ttatcggata tgataagtgc aaggtaatca agttttgggt taatttcaag atgaacatca
     5761 attttggttg cctgggcagc aattgaattt tgaactatgt cgagcaggtg caacgataaa
     5821 tccctcatat caaaatcatc taaagtatag gcagcctcat caggctagct ccctcagggg
     5881 agcataatat cgtagccgtc aatcccttta attgccttca acaagccatc gaagttcagg
     5941 ttttccatcc tgaaggtagt ggttacagac ccaacttgtt ctatgaagtg agcatcggaa
     6001 ctccttataa atctttttcg tttaagcgat gggttgtgta tgcagatact ttcaattgaa
     6061 gaatttatgg atacctccag cgcatcatag tttaggtcgg ggggtacaaa acccagctgg
     6121 ctggtcaagc tatacttcgg cctgtcaata tgagccggta tgaaaattcc attcattgca
     6181 tgcacaacag cctcaagttc gttgatgcca acatttaacg ccccatggag tgagtactca
     6241 acttcctcaa taatttcctc cttctcgtta acaactacct ggtatcctag aatatcaggt
     6301 ttattaggta ccttctggat atgtacttcc agaaaatttt gaaaatcagc tctgctatcc
     6361 tcgtcggcaa aaaggcaaac gcaatgaacc tcctcacggg tggttacctc ggcaccgtaa
     6421 atagcgagta ttcctgcatt ttcggccaaa cgacgcgtta atggcgcatg aagcgtggag
     6481 ttgtgatcag taattgcaat aatgctaagc cccatattct ttgccatgga aactatattg
     6541 ccagggctca tttcaagatc ggcacacggg gataaaacgc tgtgaatatg taaatcggcc
     6601 tggtaaaggt tcatcggtta ctgtaaagca gctggtagag cattccggaa atttcaaacg
     6661 cttgtttgga ggtccccaga atgggtattc cctcctctag gcttttctga agtgtgttgg
     6721 gttctggctc aagtcctttt accagtaaaa ttgctgcaat atccttaagt gaagctacag
     6781 ccacaatatt tatgtgcgtt tgaagggtaa tccaaacggt accagcttgt gcattgccca
     6841 ttacatcgct tagaagatcg gaggtgtagc caccggtaat ctccctgtcc atacccaccg
     6901 tttctgtata aacctttaag ccaagagact ttacaatatc tagtacggtc ataaccaatt
     6961 attttttatg tttaggttta tcgcaatcct taataaatct gttttcgccc caaatttttt
     7021 tcattatacg gaaggaatgc ttagggtcca gcttgttctg ttccatgatt ttctgtaaaa
     7081 atacgcattg tgagagcgag ccttctccct gaacaatatc ttcagccaga gcctggcacg
     7141 atggtgtacc gcatactcca cagtcaatgc caggcaggta gcaaagatac cggttgaccc
     7201 tttccatctt gttcatggct ttaaccatgc tgtcatctag cttgacaatg gagcgtggct
     7261 ccagtcggtt taagccaacc ttgccgttca gcacactact gtactctgaa atttttattt
     7321 gagtatgaaa acctatttta gattctggga tatacctggc ctgcttccga agccgttcgg
     7381 cagagaggaa gctgtttcca acagtaagta ttcctccggc acaactttca tcgcatgccc
     7441 taagctccaa gaaatcaacc ccgtgaacct cgctggtttc cattttctct agaaactcaa
     7501 taacattgga aatgccatca atggcaaggc ctttagcttc tatgctaaga gcttcaccac
     7561 ctgtcagtgc ccaagtgatg cttttcggag aaatgttaac ggcaccatct tcaaccgttc
     7621 gcttagtacc cccagatcga atatccctta gtgttcggtt gtaaattaaa tccatgttaa
     7681 taaccccatt tatttcggat acatcctccc cttcggggct tttaactgcg gcaattttgg
     7741 ccgcacatgg agtaatgtaa aaaattccaa cctcctgttc aggaattcct tcctccgtaa
     7801 gcttccttct tatgaatgat gaagtaagat ctattggggg ctttaccaac ataatgtttc
     7861 ctacaagcca ggggaatcgg acctgaacca acctgacaat tgccgggcaa aaagaagaga
     7921 ttatgggctt tcggctaggg ttgtttataa tataagcgtt tatcctggta attaggtaat
     7981 cgacggagtc ttcaacaatt actaccttag taaatcctat ccttagtaga gcgtgaatgg
     8041 ccatatcaac cgaaacatct tcgggatact gggcaagaag cactgatggt acaagagcca
     8101 ccctatactt gtagttgaaa atgctattaa agtcatcctg ttctacaata attgctccgg
     8161 ttggacaaac cctgtagcat tcaccacaat caatacacct gttgctttga agtaaggctt
     8221 ttcctcctct tactctaaga gcctcggttg ggcataccct catacaatgt gcacagccaa
     8281 tgcagacatc ggattgaatt tttaaagcgt ggtgaaatgt agaagtttcc aactctaacc
     8341 tcctctcacg ttatttactg tttaggtaaa ctacaaattc caaatgagtt cctttcccca
     8401 cctcgctgga aatgctaaat tcatcggaat tacttttaat atttggcaac cccattcctg
     8461 ctccaaagcc catctccctt acggttgaag atgcggttga aaaaccgggc tgcatggcca
     8521 gctcaatatc cgaaattcct gggccattat catcaagcct aaccagcacc ctacttgcat
     8581 caatatcaac atatgccatt ccgttatacg catgagctac aatattaacc tctgcctcat
     8641 aaagtgccac caccacccgt ttcaccagtg aaggttctac attcagctgt tttaaaattt
     8701 ttttaacctc acttgatgcg tggccggctc tcacaaaatc accaccctgt actttatatt
     8761 caaatttcat cgttatcagt aaactgcttt gatgcccaag ccgtataaaa ttccagaaac
     8821 cttaaacatg gtgcagctgc actccaccaa aactatgcta ttttcccttg ccaacctaat
     8881 catatcctcg ctcacccgct tgttccgaac aaatacgata acttgaatgt cggacatctc
     8941 ggcggtgcgt acagcttgaa tgttggacaa ccctgtaatc agcaaaaaat tctcacattt
     9001 aacagttagc acatcgctca tcagatccga ggcaaaagca taaactacct cgctgtcgag
     9061 gcactcctca cagcatagca cctttccatc tatatgcttt accagctcac ctatccgcat
     9121 aaaatatttt ttactacaat aattatacgc cacaaatata tttattgtta ttaatataac
     9181 atgtattatt aaacatgtat aatagttgcg ctatttggct aacgattata cccattgatt
     9241 aacggctgat gaaaaatcca tttttactca ttctcctact tttgttgctc atgctgggca
     9301 aattgtacaa gggcgtatga acaaccggtt gctaattgcg agttctgcaa agcctttgtg
     9361 ctggcgatcc atccgctcag ccccgtcgcc tataattgta tgccaaaata tttacaatca
     9421 gaaagctaag aatttatcag agataagcta aatgcaataa agaaggttgt actcgtactg
     9481 tgaaaaaagg aaggctgtcc ttttaagaga cagcctttaa gcatacagcc aggtactcac
     9541 tagtttttag tacctctgcc tttccttgta gtgggtatgc agtagttcat gagccttatg
     9601 gctgttgggc tcatcaagaa attccttata aatagcaatc acctcaggat tttcatgcga
     9661 ctttctgatt ttcttattag catcctcgcg gtaaattgct tcggcacgct ttttacgtat
     9721 ttcttcattg gtgggaatag gctgacctcc acctccgatg caccccccag ggcaagccat
     9781 aatctcaatg aagtgccagt cggcttttcc cgacttaacc acatccatca cctttttggc
     9841 attggtcaat ccgtgagcta caacggtttt aagaaccacg ccatcaagga acttccattc
     9901 gggtttggta ttctcaattt taatggcagc ctcacgaaca ccctccattc ccctaacagg
     9961 tgtaatgttg aggccatcaa atggaacctc tctaccggta actacctcgt aggcggttcg
    10021 tagggcagcc tccataaccc ctccggtagc accaaaaata acagcagcac ctgtggaatc
    10081 tcccataatg ctatcgtagt ttgtttccgg taggctgtca aactctatac ctgcctgctt
    10141 gatcataata gccagctcac gggtggtgag cacatagtcc acatccttat aaccgctgga
    10201 gtgcatttcc ggtcggtcag cctcgaattt cttggccgtg cagggcataa tggaaaccga
    10261 aactatactg gccggatcaa tgttacgttt tttagcgtag taggttttgg ccagagcccc
    10321 aaacatttgt tggggtgatt tacaggtgga aaggttgtca agatactccg gataggtatg
    10381 ttcaataaac ttaatccaac cgggagagca gctcgttgcc atgggaatct tcacagtact
    10441 atccttgtca accaatgcct ttttgagcct gccaagcaac tcgaatccct cctccataat
    10501 ggttaggtcg gctgaaaaat cagtatccaa aactgaatca aaccctagcc ttctaagggc
    10561 ggttaccatt ttgccggtaa cgatttgacc aggtttcatc cccagcgcct ctccaagggc
    10621 aacccttaca gccggtgcag tttgaacaac aacatgcttg ttagggttat aaatggcatc
    10681 ccaaacctcc tccacgtagt tgcgttcaac cagcgctccg gttggacagc ggtttacgca
    10741 ttggccgcag ttggtacaaa ccacctcgaa catgggattt tcgaaaaagg tggaaatttt
    10801 catgttggca cctttatatg caacccccag tgcgctaaca tgctggagtt cttcgcaggt
    10861 acgcacgcaa cgctgacaac ggatgcactt gctgtcatcc ttaattattg atggtgaata
    10921 ggcgtccatc tggtaatttt tgtatggaac cagatcgata aacatgggct cggaaatttt
    10981 gtattccgag gccaggtttt gcagctcgca attaccgttc ttgtagcatg tggtgcactt
    11041 ggcgttgtgc tcggagagca agaggtcaat gatgtgctta cgggcggtgc gaaccttaat
    11101 tgtatttgta aaaatttcca ttccctccgc agcaggcatg gcgcatgaag caggaagtgt
    11161 tttcgatccc ttcatttcaa ccacgcaaac acgacaattt cctgccacgc ataaatcgtc
    11221 atgataacat agcgttggaa tgtggatatt tagtttttta gcagcctgta gaattgtagt
    11281 tccctcttca acagtgattg ggaaaccatc tatggtgagg tttatttgtt tagccatgaa
    11341 tttgaaattt taatttagta aatcatctct tccttaaagt tctccacaat agagaagaac
    11401 gaatttgcca cgctttgccc caaaccgcat tttgcggtta tcttcattgt ttgggcgagt
    11461 ttctgcagct gatcgaggta tgcagcatgg cgatcgccct tcttaaccgc ttcaatacca
    11521 agtataagct gctggcatcc aacgcgacac ggagtgcact gtccacatga ctcctcggaa
    11581 aagaaatcca ggtaattttt aagcacgttg tacatggatc gcgagctgtt aaagagcatc
    11641 atggaacctc cagtaggaac accctcaaat ccgattatgg tatccttgaa cttttttcgg
    11701 ggaacgcaaa accctgaagc gccacccacc tgaaccgctt tggtatcacc atctccaaac
    11761 tcgtcaacaa attgctcgac ggtcatacct agctccaact cataaattcc aggttttgga
    11821 gtatctccag atacagaaaa caccttagac ccgcgtgaat ctgcagttcc cagctccttg
    11881 aatttgcggg cgccatagcg gaaaatcata aaggcataaa ccagcgtttc aacgttgttg
    11941 ataacggtag gttttttccg gaatcccgat acggtagggt atggaggttt gttgcgaggt
    12001 tcgcccctgt cgccttccat cgattcgaaa agagcgcttt cctcaccgca tatataggcg
    12061 ccactaccca ctataatttt tattttaaaa tcaattttca aatctttcaa aatctcgttg
    12121 aacagcttta gctgattttc cagctccgaa agtaaaaagc gatactctcc tctaaggtag
    12181 ataaatcctt ccttggcgtt gataaccttg gcacagatag ccattccgcc aaacacctta
    12241 aggggaacct taaagagaat ctcacggtcc ttaaaggttc caggttcgcc ctcgtcggca
    12301 ttgcaaacca cgtacttttc cggatcagcc tgctcgctgg taaacttcca tttaagggca
    12361 gttgggaaac cggcacctcc ccttccttta agccctgatt caagtagctg gttgataatc
    12421 tcgtcgttgg aatgctggta tgccgatttc aacacctcct taaagttgca atcctgatca
    12481 aatatcaggt caacacgtcg caaaaaatta ctggccatga ttatagtctc ctctactttt
    12541 tcatgtaatc attaatgatt tctctaactt tttcgggcgt tagctcggtg taagcctcct
    12601 cgttaacaag catggccggg cccttatggc accagcccag gcagttggtt tcaagcagcg
    12661 aaaacttacg gttttgcgta gtttcaccta cctttatctt caaaatatcc tccaactcct
    12721 gtaaaattgg gtttttaccc ttcatggcgc atgaaattgt cttgcaaacc ctaattacat
    12781 actttccccg aggctgggtg tcgagaaagc tgtagaaggt ggctgttcca taaactttag
    12841 cagccgaaat gtccagttcc ctagcaattt cggtcatcgc ctcgctagaa atgtaccgtt
    12901 catgctgcac cacaccctgt aatatgggta ttaagctctc ccttgatcgc ccatgcttgt
    12961 cagcaagctt tttaactaac tcaacggttt ccgtcataat ccaaaaattt aatagttttt
    13021 attgttataa cgataacatt gttaaaataa taacactaaa tttcattcac acagtaacat
    13081 ttgacttgcc taaatataaa aactatttag gttccaaaaa actgattttt gtcataaata
    13141 taaccatgat ggataaaaat ttcaggttaa tgattggtgc tctaccataa cttttaatgg
    13201 aattttgaga gataataaac ccggacaaag gttataaccg ttgcgatcta ttactgccct
    13261 acacgaaata ctatctatat gaattactga ctaaagcttt atgcgagtat gcctaacctt
    13321 ttcaacaccc tagcacatag cctctatatt ctgtttcaac aattttaaaa ataatcatgc
    13381 agataattta ttcaatatta aatacttata tatttttaga ttaaataaat gggaacgtga
    13441 tacttaataa ttgtcggata acaaaactat ccttatgcct aaaaaatact ttaatcaatg
    13501 gtgctcaata ataatacttt gttaaaaata ttatactttt gttgaataac ata
//
LOCUS       DAOPHX010000065        13362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_5417_length_13362_cov_6.515142, whole genome shotgun sequence.
ACCESSION   DAOPHX010000065 DAOPHX010000000
VERSION     DAOPHX010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 13362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13362
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5417_length_13362_cov_6.515142"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..2571
                     /locus_tag="PK502_06225"
     CDS             190..2571
                     /locus_tag="PK502_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPH00446.1"
                     /translation="MKHDSTTTVWDKYSCSKGWQFQLTKAAKIVALASALMLFFPIIT
                     HAQNSITGTIKDKATGDPLAGVHVSVGQQVATTNSQGFYVLKNLKPGTHIVTASLVGY
                     KSEQQPAKIVNESITLNFFLEEDLIFLNEVTVSATRTESRIADIPGRIEMITPEKISL
                     ASYQSVDELLTLIPGVHTARSFGLFSYRSSVSMRGVSSKEQGRTLVLLDGIPMNKADG
                     GSVNWNLISTREIERIEVVKGPGSALYGGNAMGGIINVVTQKPTRPLEGSVAGSYGTY
                     NTKGVNAKLAGKLNNGFYWAADGLFRNSDGYITQSIADRLTNPYIVKSTFEEKVLHLR
                     TGYDNGQKLQADVDFTLFDDVRTTGEKVYQPQGNTREYDTYQLRANVKGVAGAVVWNV
                     SAFFLNEDYKRVSEWMKDDYTWYDVLSVRRDYGLLTSANYRAGRHTFTGGVDLRMGSV
                     DASDVYYTSTDRVDNNGKMNFYGFYLQDVVALFDSTVNVTAGLRYDYARFFDGQFQIL
                     SPSAETAFMSQYQFSQNGSESWGALSPRISAQFKPNGNYRIYASYSRGFRPSVLDDLC
                     RSGRVKGGFKVANPNLKPEYLDNFEVGGDYKPFDRLRLAASAYYSTGKDFLYYVSTGD
                     SIDMGFGPRPIMIPSNISDVEIYGMEAEFSATPWRFLTLSGGYAYALSKITGYKPIAA
                     NDFDLTGKYLTDVPKHSFNAGAFLRSHIVNAGVTANYTGRMYINDQNAYDDIVLSNQL
                     PAAFTLDLKISREFFGHVNISLNIQNILDKKIYESSMSVGPGRFMMLEVKANL"
     gene            2626..3690
                     /locus_tag="PK502_06230"
     CDS             2626..3690
                     /locus_tag="PK502_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00447.1"
                     /translation="MKRIILAASALFMASLLPSCGNHPDGISGATKDYDSNGNSFYHR
                     TTETKLKTCDLRVEGEVRKPGEINLKHYFKREVVYKQAVLNDSGQVAFTGAYRYRGYS
                     LFDLLNPFVLEKKNAKEFVPATDVYIIIENNSGDRVVFSWAEIFLGHNMHQVLIATEQ
                     ADVEPYKVKVSYPKDSVWKVVAANDLFAYRELKNPSRIIVKSFDRKYYEINRELKDPF
                     SPTVNLVVDDSLMGVIDTLNAVAPHARYHSVFYGMGMGYHGTPTFEGPLLRPLVENFL
                     AKDGAKWMRTGIACVVGKDGFRNIFSVSELFNRVDQVEPILAIPENPKKSGYYRLYHP
                     SAFYADFSVRNLSEIYLFRE"
     gene            complement(3757..4686)
                     /gene="miaA"
                     /locus_tag="PK502_06235"
     CDS             complement(3757..4686)
                     /gene="miaA"
                     /locus_tag="PK502_06235"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPH00448.1"
                     /translation="MDNCPYDIIAIVGPTASGKTLLAANLASRVNGEIISADSRQVYR
                     GMNLGTGKDYEDYVVNGKTIPYHLVDLVDAGYHYNVYEYQSDFLKVFRSLKARKVLPI
                     LCGGSGLYIEAVLRGYRLLPVPQNATLRGELEAKSNDELAKILASYKTLHNTTDLDTR
                     KRTIRAIEIEEHYLNAPTEIEPFPQLKSAIFGVRYNRATEMERIHQRLLQRLGNGMVD
                     EVRGLMESGLSFDDMAYYGLEYRYIAEHIMGKLTYDEMVSRLNIAIRQFAKRQMTWFR
                     GMERRGLSITWINGELPLEDKLNKIMEAIQNGF"
     gene            4806..5039
                     /locus_tag="PK502_06240"
     CDS             4806..5039
                     /locus_tag="PK502_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00449.1"
                     /translation="MAALRRNNRQSRHEELNRLIWMGIVIAVTFIFVIAVATDMFRII
                     PLKRTNIVMLVLALLIVAVYVYSWIKFYRKRKQ"
     gene            5052..5501
                     /gene="smpB"
                     /locus_tag="PK502_06245"
     CDS             5052..5501
                     /gene="smpB"
                     /locus_tag="PK502_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPH00450.1"
                     /translation="MSNEIVVKNKKATFQFEIVEKFTAGIVLKGTEIKSIRMGRASFV
                     DSYCFFIGDELWVKGLHIAEYAWGTYNNHDPKQDRKLLLTRKELTKLKRRSQEKGLTI
                     IALKLFINERGLAKVDIALARGKQLHDKRDDLREKDVRRELDRARKR"
     gene            5639..6238
                     /locus_tag="PK502_06250"
     CDS             5639..6238
                     /locus_tag="PK502_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPH00451.1"
                     /translation="MGKVKLTVLGISYSQTQSGAYALVLSEEDGNRRIPIIIGGYEAQ
                     AIAIQLEGLTPPRPLTHDLFMNFAKAYHIEILDVNIYKLEEGVFFSKLHCTNGDKEIF
                     VDSRTSDAVALALRFDCPIYTTEEIIEKAGLTLEIENIDEDAAKEDEGEKGNAPVDSF
                     MEQLAKLSVSELKTMLDEAVRNEDYEKASKIRDELKNRS"
     gene            complement(6321..7562)
                     /locus_tag="PK502_06255"
     CDS             complement(6321..7562)
                     /locus_tag="PK502_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HPH00452.1"
                     /translation="MKLKLRLTLMSFLQYAIWGSWLISLGAYLGGGLHFSGFQIGSFF
                     ATMGIASLFMPAIMGIIADRWIPAQKVLGICHILAAAFLIAAAPQTQYGTLYPLVLLS
                     VMFYMPTIALSNSVAYNALSLNGYDTVKAFPPIRVWGTVGFICAMILVDLLGFATTSY
                     QFYLSAGLGLILGIYSFTLPNCEVNKNVEKKSLVDSLGLRAFTLFKERKMAVFFIFAM
                     LLGMSLQITNAYANDYLTNYFGKISEYQGTFGVKHANILISLSQISETLCILLIPFFL
                     SRFGIKKVMLISMFAWVLRFGLLGLGNPGSGVWMLILSMIVYGVAFDFFNISGSLFVE
                     NETDAKIRSSAQGVFMIMTNGFGAFIGSYIAGLVVDATDWPDAWFVFSAYSLVVAIVF
                     AFVFKYKHNPKNSSTVSKAAQ"
     gene            8104..9174
                     /gene="lpxK"
                     /locus_tag="PK502_06260"
     CDS             8104..9174
                     /gene="lpxK"
                     /locus_tag="PK502_06260"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPH00453.1"
                     /translation="MRQIRLLLLPIAWLYWCGVRIRNILFDAGILKGFSPDIPTICVG
                     NLAVGGTGKTPMTAYLSSLLHGNFNVAVLSRGYKRKTKGFNIVSTESTVHEVGDEPLL
                     LKLRQPSAIVAVDEDRMNGMVELFYAYPELDAIILDDAFQHRWIKPGLNLLLTEYENL
                     YTHDFPLPAGRLRDSLSERKRANLIIVTKCPENLTADRCREISAELKPNPTQIIYFTS
                     MEYGMPQPVFSESAGKLKLTDMQPVFAMTGIANPKPFVDYLQKHTALLAAKAFADHHY
                     YSEDEIEAIFTELARSTSQAVVVTTEKDAVRLLKLKLPEEIKKRIYYIPVSVKFLFNS
                     SEEFNNQILSYVRENKSDSSFH"
     gene            9137..9946
                     /locus_tag="PK502_06265"
     CDS             9137..9946
                     /locus_tag="PK502_06265"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPH00454.1"
                     /translation="MLEKINQTVAFIKGKVTIEPEVGIILGTGLGGLVKEIEKQQALD
                     YKDIPNFPISTVEGHTGRLIFGELGGKKVVAMQGRFHFYEGYDMKQVTFPVRVMKFLG
                     IQYLFVSNASGGMNPAFEIGDLMIINDHINLMPNPLLGPNISELGPRFPDMHRAYDEK
                     LIALAKKVAASNGTAIKEGCYVGTTGPTFETPKEYQYFRIIGGDAVGMSTVPEVIVAR
                     HMNIPVFAISIITDLGVPGKIIEVSHEDVQKIGNLSEQKMTRIMKDMLTQL"
     gene            complement(10023..10550)
                     /locus_tag="PK502_06270"
     CDS             complement(10023..10550)
                     /locus_tag="PK502_06270"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF010555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPH00455.1"
                     /translation="MKIFLIGFMASGKTTIGRELASALQYKFIDLDDFIESRHNQTIK
                     QLFEFKGEDYFRKVENEALKEVCDYKDDYVISAGGGTSCFYNNIDFMNKNGITVYLRM
                     EVATLVSRLIESKIDRPLLWGKTGDELANYILRVLDERKKFYNKAQLVVDGDNLNPAE
                     LAQLISAIVKEEKLL"
     gene            10942..13269
                     /locus_tag="PK502_06275"
     CDS             10942..13269
                     /locus_tag="PK502_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPH00456.1"
                     /translation="MKVKMRIGVKMLLFVMSTSVLVMLVIGLFIGFRINKIARDDAVR
                     IVQSEAQKTASQVKSELELDMGFSRALAHALYIYPKFDTLTLDSIFINILKNQVANNP
                     RYLTVWYSIEYSAYRPGYTKNYGRRSVTAYLRDGNIGVDIEHKNVTGDVTTSNYYSSK
                     TCNCEMLLDPYTFVYGGREVLATSLSVPVRVGDRFAGLGGVDISLEKFQETIEKIKPY
                     SNTRVSLIASDGTIIADTNPENARLKFSELFPSEEATYQLSQKIKNGLDFQYLTIQDN
                     REFLNIITPVKVGDSPTYWSVCLSIPLDVVVAEARKAVIYTILVFLFGIILQTLAIWY
                     ISNYITRPIKKTTGVLNNLAEGDIDINSKIYIHTGDELEEMARSTSKLIDGLNHTVQF
                     AREIGQGKLDSEFTLLSSKDRLGEALIDMQKSLLKAREAEVQRKDEENKQNWATRGVA
                     MFGEVLRQHNDNLNELSYLIVKNLVSYTGSIQGGIYLINDNDSDNPFLEMTACYAYDR
                     RKFMEKNILPNEGLVGRCYVEGKTILMTDVPKNYIKITSGLGGENPRCLLIVPLKVND
                     TVLGVIELASFNPYQKYQVDFIEKIAESIAATLSSVRVNIRTAELLERTQQQAEEMKA
                     QEEEMRQNMEELHATQEEMERKRLEQESINTQLAEDRTILNSLMQNLPDCMFQKDVNG
                     RYVRLSNSMLNLLGIDQPDEVIGLTDFDLFPTSLAKLLSKLDSEVLSSRQPITSKPIE
                     LVLKNGVEQDALLTLMPLVEENGDAIGVMGVMKLA"
BASE COUNT         3901 a         3089 c         3033 g         3339 t
ORIGIN      
        1 atgccgatca ggttgcagaa ggatacatgg agctggaaat taaaattcag gagtagttga
       61 gaatggttgc tgctaattaa acggcatcac ctgtataaaa aagggaaggt aaaattattt
      121 ccataggaaa gcatagaaat agaagtaaaa atcaacaaag gtgaaattat aacacaggaa
      181 aaattagtga tgaaacacga tagcactact actgtttggg ataaatacag ctgttcaaag
      241 ggctggcaat ttcaactaac taaggccgct aaaatagtag ctttagcttc ggcattgatg
      301 ctgttcttcc ccatcatcac ccatgcccaa aactccatca cgggaaccat taaggataag
      361 gcaaccggcg atccccttgc aggggttcat gtgtcggttg gacaacaggt tgccaccact
      421 aactcgcaag gtttttatgt attgaaaaac ctaaaacccg gaacccacat agttactgcc
      481 tcactggtag ggtataagtc agaacaacaa cctgccaaga ttgtaaacga atcaatcacc
      541 ctcaactttt ttcttgagga agatttgata tttctcaacg aggtaaccgt ttcggctacc
      601 cgaaccgaaa gccgaatagc agatattcct ggccgtattg agatgataac ccccgagaaa
      661 atatcgcttg cctcctacca gagcgttgac gagttgctga cgcttattcc tggggtgcat
      721 acagcacgct cattcggact tttctcctac cgctcatccg tttcgatgag gggcgtaagc
      781 tctaaggagc agggacgaac ccttgtgctg ctcgatggaa tcccaatgaa taaggccgat
      841 ggtggctctg tgaactggaa cctgatatca acaagagaaa tcgagcgcat agaggtggtt
      901 aaagggccag gttccgccct ttacggtgga aatgccatgg gtggaattat taatgttgtt
      961 acccaaaaac ctacccgtcc attggagggt agcgttgcgg gcagctatgg cacctacaac
     1021 accaaagggg tgaacgctaa actggcaggc aagctgaaca acggattcta ctgggcggcc
     1081 gatgggttat tccgcaacag cgacggctac attacccagt caatagccga caggctcaca
     1141 aacccataca ttgtgaaatc cacctttgag gaaaaggtgc tgcatctgag aaccgggtat
     1201 gataatgggc agaagctgca ggccgatgta gattttacgc tgtttgacga tgttcgcaca
     1261 actggcgaaa aggtttacca gccgcagggc aacacacgtg agtacgacac ctaccagctt
     1321 cgggcaaacg taaagggagt tgccggagcc gttgtgtgga acgtttcggc attctttctg
     1381 aatgaggact acaagcgcgt aagcgaatgg atgaaagatg actacacttg gtacgatgtg
     1441 ctttccgttc gtcgcgatta tggattgctc accagcgcaa actacagagc aggaaggcat
     1501 acgtttaccg gtggagttga cctgagaatg ggcagcgttg atgcctccga tgtttactac
     1561 acctcaaccg atcgggttga caacaacggc aagatgaact tttacggttt ctacctgcaa
     1621 gatgtagtgg ctcttttcga cagcaccgtg aatgtaactg ccggtttgag gtacgactac
     1681 gcacgattct tcgacggcca attccagatt ctttcacctt cagccgaaac agctttcatg
     1741 agccagtacc agttttccca gaacggttca gaaagctggg gggcattaag tccacgaatt
     1801 tctgcccaat tcaaacctaa cggcaactac agaatatacg ccagctacag caggggtttc
     1861 agaccttccg tccttgacga tctatgccgc tcgggacgcg ttaagggtgg ttttaaggta
     1921 gcaaacccca accttaaacc cgaatacctc gacaacttcg aagtgggggg cgattataaa
     1981 ccatttgatc gcttacgctt agccgcaagc gcctactatt ccactggcaa agatttccta
     2041 tactacgttt ccaccggcga cagcattgac atggggtttg gacctcgacc aataatgata
     2101 ccctccaaca tttcggatgt ggaaatatac ggcatggagg cagaattttc ggctactccc
     2161 tggaggttcc tcaccctgtc gggaggctat gcctacgcct tatccaaaat tacaggatat
     2221 aagcctatcg cagccaacga ctttgacctt accggaaagt atcttaccga tgtgccaaaa
     2281 cactcgttca acgccggagc attcctgcgt tcgcatattg taaatgcagg agttaccgcc
     2341 aactacaccg gcagaatgta catcaacgac caaaacgctt atgatgatat agttctgagc
     2401 aaccagcttc cggcagcctt taccctcgac ctgaagatct cccgtgaatt tttcggccat
     2461 gtgaacatct cacttaacat tcagaatatt ctggataaaa aaatttacga aagtagcatg
     2521 tcggttggcc cgggacgttt catgatgctg gaggtaaagg caaacctata gttttatacc
     2581 tatttgggat aaccataatt gtattttatc taacctgata aactgatgaa gagaattata
     2641 cttgctgcaa gtgcgttgtt catggcatca ctactgccat cgtgcggaaa ccatcccgat
     2701 ggcatatcgg gagcaaccaa ggattacgac tcgaacggaa acagcttcta ccaccggaca
     2761 accgaaacca agctaaaaac ctgcgactta cgggtagagg gcgaggttcg taaacccggg
     2821 gaaatcaacc ttaaacacta cttcaagcga gaggtggttt acaagcaggc agtgctgaac
     2881 gattcaggac aagtagcgtt taccggcgca taccgctacc ggggctactc ccttttcgat
     2941 ctgcttaacc cgtttgtcct ggagaaaaaa aacgccaagg aatttgttcc ggcaaccgat
     3001 gtttacatca ttattgaaaa caattcgggc gacagggtgg tattctcatg ggctgaaatt
     3061 ttccttggtc acaacatgca ccaggtgcta attgccaccg aacaggccga cgttgaaccc
     3121 tacaaggtta aagtcagcta cccgaaggac agtgtttgga aggtagtggc agccaacgac
     3181 ttgtttgctt accgcgagtt gaaaaatcct tcaagaataa ttgtgaagtc attcgacagg
     3241 aagtactatg aaatcaacag ggagctgaaa gatccattta gcccaacggt taacttggtg
     3301 gttgacgact ccctgatggg agttatagac accctgaacg cagttgcacc tcatgcccgc
     3361 taccactcag tgttttacgg tatgggcatg ggctaccacg gtacgccaac ctttgaggga
     3421 ccgctgcttc gacccttggt tgaaaatttc ctcgccaaag acggggcaaa atggatgcgg
     3481 acaggcatag cctgcgtggt gggaaaagat ggttttagaa acatatttag cgtaagcgag
     3541 ctgttcaacc gggttgatca ggttgaaccg attctggcca tacccgaaaa tccgaagaaa
     3601 agtggttact accgtcttta ccacccgtca gctttttacg ccgatttctc ggtaagaaac
     3661 ctgtcggaga tttacctgtt ccgggagtga gagggttgct gtgttgcccc tcctgtttgg
     3721 aatgccgttt tatcgggaaa agcatcattc aaacagctag aaaccatttt gaatggcctc
     3781 cattatcttg ttcagcttat cttccaaagg gagttcgcca ttaatccagg taatgcttag
     3841 cccacggcgt tccatgccac gaaaccaggt catttgccgc ttggcaaatt gcctgatagc
     3901 gatgttcaag cggctaacca tttcgtcgta ggttaacttg cccatgatgt gctcggcgat
     3961 gtagcggtac tccaagccgt agtaagccat atcgtcaaaa cttagccccg actccatcaa
     4021 ccccctcacc tcatctacca taccgttccc gagccgttgt agcagccgct gatggattcg
     4081 ctccatttcg gtagcccggt tgtaccttac gccgaatatg gcacttttaa gctgagggaa
     4141 tggttcaatt tcggtaggag cgttcaggta gtgctcctca atttcgatag ccctgatagt
     4201 tcgcttacgg gtgtcgagat cggtggtgtt gtgaagagtt ttgtaggatg caagaatttt
     4261 agccagctcg tcgttcgact tagcctcaag ctccccacgg agggttgcgt tctggggaac
     4321 tggcaaaagc cggtaccccc ttaacacagc ctctatgtaa agcccgcttc ctccacaaag
     4381 tataggcaaa accttgcgcg ctttcaggct acggaatacc ttcagaaaat cggactggta
     4441 ctcataaaca ttgtagtgat agccggcatc aaccaagtca accagatggt agggtatcgt
     4501 tttgccattt accacgtagt cctcatagtc tttaccggtt ccaaggttca tgccgcggta
     4561 aacctgtcgg gagtcggcgc taataatttc tccatttacc cgcgatgcca gattagctgc
     4621 aagcagggtt ttaccactag cagttgggcc tacaatcgca ataatatcgt acgggcaatt
     4681 gtccatttat cttttgggtg ataaagcgta aatttagcta tttttgcgcc ctacaaacat
     4741 tctaacagcc aagtcaccat cctatcccaa tttataattt tatccacctt taaataacca
     4801 gcaccatggc agcgctacga aggaataacc ggcaaagcag gcatgaagag ctaaacaggc
     4861 taatctggat gggaattgta attgccgtta cattcatatt tgttattgct gttgcaacag
     4921 acatgttcag gataattccg cttaaacgaa ccaacattgt aatgctagtg ttggcattgc
     4981 taattgtggc ggtttatgtt tacagctgga taaagtttta cagaaaaagg aagcagtagc
     5041 cacatactcc catgagtaac gaaattgttg ttaaaaacaa gaaggcaacc tttcagttcg
     5101 agattgtcga aaagttcacc gccggaattg tgcttaaagg aacggaaatt aaatccatcc
     5161 gaatgggcag ggccagcttt gtggattcgt actgcttttt cattggtgat gaactatggg
     5221 ttaaggggct tcatatagcg gagtatgcct ggggtaccta caacaaccac gaccccaaac
     5281 aggatagaaa gctgctgtta accagaaagg agctcactaa gctgaaacgg cggtcacagg
     5341 agaaaggact caccattata gccctgaaac tttttatcaa cgaacggggt ttggccaagg
     5401 ttgatattgc tttggcacgg ggaaagcagc tgcacgacaa gcgggacgac ctgagggaaa
     5461 aggacgttcg tcgtgagctc gatcgtgccc gtaaaaggta gccatcatct tttttcttac
     5521 cctgtttctt ctaaaggatt ttcattctca agctaattac cagcgcataa aattcaaatt
     5581 ttgaaaaaat tccattaggt ttgtaaagtg caataaaaaa ggataaccag caaagcaaat
     5641 gggtaaggta aagttaaccg ttttaggtat ttcatacagt caaactcagt ctggtgccta
     5701 cgccttagtc ttgagcgaag aagacgggaa caggagaatt ccgataataa ttggtggcta
     5761 cgaggcgcaa gccattgcaa ttcaactcga ggggttaacc ccaccacgcc cgttgaccca
     5821 cgacttattc atgaattttg ctaaagctta ccatatagaa attctggatg tcaacatcta
     5881 caagctggag gagggtgtgt ttttttcaaa actacactgc accaatggcg ataaggagat
     5941 atttgttgat tccagaacct ccgatgctgt agcgttggca ctaaggtttg attgccccat
     6001 ctacaccacc gaggaaataa ttgaaaaagc cgggcttacc ctcgaaattg agaatattga
     6061 cgaggatgcc gctaaggagg atgagggaga aaagggaaat gcccccgttg acagcttcat
     6121 ggagcaactc gccaagctat ctgtttcgga actaaagact atgctcgacg aagccgttcg
     6181 gaacgaggac tacgaaaagg cttcaaaaat tcgcgacgag ctaaagaacc gctcctgaaa
     6241 aaaataaagt caaacattac ctatgggcat caagcagaag ccccatcctg gcaggatggg
     6301 gcttctggca aaatacttct ctattgtgca gccttactta cggtggacga attctttggg
     6361 ttgtgcttgt acttaaaaac aaacgcaaac acaattgcca ctaccaacga gtaggccgaa
     6421 aatacaaacc aggcatcagg ccagtctgtt gcatcaacaa ccagccctgc aatataggag
     6481 cctataaatg ctccaaaacc attggtcata atcataaaaa ccccctgcgc gctggaacgg
     6541 attttcgcat cggtctcatt ttccacaaaa agtgaccccg aaatgttgaa aaagtcgaat
     6601 gcaacgccat acacaatcat ggaaagaatc agcatccaaa ctcctgaacc tgggttcccc
     6661 aaccccagca gaccaaaacg aagtacccat gcaaacattg atatcagcat aaccttttta
     6721 ataccaaacc ggctaaggaa aaacggtatc agcagaatgc aaagggtttc tgaaatctgc
     6781 gatagcgaga tgagtatgtt ggcatgttta acaccaaatg ttccctggta ctcgctaatt
     6841 ttgccaaagt agttggtgag gtagtcgttg gcgtaggcgt tggttatctg caacgacatg
     6901 cctagcagca tggcaaaaat aaagaatacg gccatcttgc gctccttaaa caaggtaaag
     6961 gcacgcagcc ctaaagagtc aaccagcgat tttttctcta catttttatt cacctcacag
     7021 ttggggagcg taaatgagta aattcctaaa attaacccca gccccgccga aaggtaaaac
     7081 tggtaggagg ttgtggcaaa gcccagcagg tcaaccaaaa tcatagcgca aataaaaccc
     7141 accgttcccc atacccgaat gggaggaaat gcctttaccg tatcgtaccc attgagcgat
     7201 agggcattat aggcaaccga gtttgatagg gctatagtag gcatgtagaa cataacgctt
     7261 agcagtacca gtggatacaa ggtgccgtac tgcgtttggg gagcagcagc aatcagaaag
     7321 gcggctgcaa gaatatggca aatgcccagc accttttggg caggaatcca cctatcggca
     7381 atgatgccca ttattgcagg catgaaaagc gaagcaatac ccatcgttgc aaaaaaactc
     7441 ccaatctgga aacccgaaaa atgtaagcct cctcccaaat atgcaccgag ggaaattaac
     7501 cacgaacccc aaatggcata ctgcagaaag ctcatcagcg ttaaacgcaa ctttaacttc
     7561 ataacttttt atatttagcg ttatcgattt ttttaaagtc ctaaaaatag cattgtttta
     7621 tttataaggt tttcgataag ggtagaaatg ttttctgtta aaagggcata tcacagcata
     7681 aactaaacat atccaataaa tattttatga aatttaccgc atttttccac ccttttgcat
     7741 aacaatgatg gccatattta attttaaccc acaagcacat gctaaagcac cactagctca
     7801 agcccaattt ctaaaaatat cgtcggaatg aatatggcaa cacctcagca gatactcacc
     7861 aaagcaaaca ggctaaagct taaatacccg gtttgctttt ccattgctac catgtaggct
     7921 ggtagctgca gccaatccgc attgaacctg caatatgttt tttttcttct acatcctaaa
     7981 tgtggaagat tttccactaa atttcgggca aagcgctgta gtgttgtcaa taaaatataa
     8041 ttttatccca aatttattgc tggctttgat tgctagccta caataagaaa gttcaacatt
     8101 aacatgcggc aaatcaggct acttttactt cccattgcat ggctttactg gtgcggagta
     8161 agaattcgca acatcctgtt cgatgcaggt attctcaagg gtttcagtcc cgacattcca
     8221 accatttgcg tgggtaacct ggctgtaggg ggaaccggca aaacacccat gacggcctac
     8281 ctttcatccc tactgcatgg aaattttaat gttgcagtgc taagccgggg gtataaaaga
     8341 aagaccaagg gtttcaatat agtaagtacc gaatcaaccg ttcacgaagt aggcgacgag
     8401 cctcttttgc taaaactccg tcaaccgtcg gctatcgtag ccgttgacga ggaccgtatg
     8461 aatggcatgg tggagttgtt ttacgcctat cccgagctgg atgcaattat ccttgacgat
     8521 gcctttcagc accgttggat aaaaccagga ttaaacctgc ttttgaccga atatgagaac
     8581 ctttacaccc acgattttcc ccttccggca ggcaggctac gcgacagcct atcggagcga
     8641 aaacgtgcaa atctcattat tgttaccaaa tgccccgaaa acctcaccgc cgataggtgc
     8701 agggagattt ccgcagaact taagccaaac ccaactcaaa tcatttactt caccagcatg
     8761 gagtacggta tgccccaacc agtatttagc gaaagcgcag ggaaacttaa gctaaccgac
     8821 atgcaaccgg tttttgccat gacgggaatt gccaacccca aacctttcgt tgattacctg
     8881 caaaagcata cagcattgct tgccgccaaa gcatttgccg atcaccatta ctattccgaa
     8941 gatgagatcg aggctatttt tacggagctt gccaggtcaa ccagccaggc tgtagtggtt
     9001 acaactgaaa aagacgcagt tcgcctgctg aagttgaagt tgccggagga aattaaaaaa
     9061 cggatatact atattccggt gagcgtgaag tttctattca acagttcaga agaatttaat
     9121 aaccaaatac ttagttatgt tagagaaaat aaatcagaca gtagctttca ttaaggggaa
     9181 ggttaccatt gaacccgaag ttggaattat tcttggaacc gggcttggcg gcctggtaaa
     9241 ggagatagaa aagcaacagg cattggatta caaggatatt ccaaactttc cgatatcaac
     9301 ggttgaaggg cacaccggac gcctgatttt cggtgagcta ggtggtaaga aagtggtggc
     9361 catgcaggga cgcttccact tctacgaagg ttatgatatg aagcaggtta ccttccctgt
     9421 gagggtgatg aagtttctgg gcatccagta cctttttgta tcgaatgcca gcggtggtat
     9481 gaatcccgcc tttgaaattg gtgacctgat gatcatcaac gaccacatca acctgatgcc
     9541 taatcccctt cttgggccta atataagtga gctaggtcca agattcccgg atatgcatag
     9601 ggcttacgat gagaagctga ttgccctggc aaaaaaggtt gcagcatcaa acggcactgc
     9661 aattaaggaa ggatgctacg ttggaaccac cgggccaacc tttgaaaccc caaaggaata
     9721 ccagtacttt agaattattg gcggtgatgc agttggcatg tcaaccgttc cggaggtaat
     9781 tgtagctcgg cacatgaata taccagtttt tgccatctcg attataaccg acctgggcgt
     9841 tccgggcaaa attatcgagg tcagccatga ggatgttcaa aaaattggca acctctcgga
     9901 gcagaagatg acccgaataa tgaaagatat gcttacacag ctttaacaag agctatgttt
     9961 tatgcgaaag aatcatgggg cggttagagg ggttacccct tctgccccat gctaattttt
    10021 agctacaaca atttctcctc ctttacaatt gccgaaatca actgggcaag ttcagcaggg
    10081 ttaaggttgt ctccatcaac caccagctga gccttgttgt aaaacttttt ccgttcgtcg
    10141 agcaccctga ggatatagtt agccagctca tcaccagttt ttccccaaag taatgggcgg
    10201 tcaatcttcg attcaattaa acgggaaacc agtgtagcca cctccattcg caggtaaacg
    10261 gttataccgt tcttgttcat aaagtcaata ttgttgtaaa agcatgaagt gccgccacca
    10321 gctgagatga catagtcgtc tttgtaatca caaacctcct taagggcttc attttcaacc
    10381 ttccggaagt agtcttctcc tttaaactca aacagctgct tgatggtttg gttatgcctg
    10441 ctttcgataa aatcatctaa atctataaat ttatactgca gtgcgctggc cagctcccga
    10501 ccaatagtcg ttttaccgct tgccataaat ccaattaaaa atattttcat gttagtggtt
    10561 ttttaaatca acctaatcct tcaaaaattg ctgcctaaat ctctataaaa aaatacaaaa
    10621 cagttcaagt tgaaaatata agggttacaa gccgtctatt ttacaagaaa tgtgaaaaaa
    10681 aggttacaaa ttgcatggtg ctaccctccc gcttggcatt gtttacggtt ataacgttta
    10741 ttcgaacctg taattcaagc cttatggaaa cttgcagcta agactacccc ctcgctgagc
    10801 ttgttgtaac ctcaaaaaat ttgattagat tggcaataca atacaaattt ttgcgcacca
    10861 acacctcaac ctaaaaacat ttcgtatttt taagcggata tttagctggt gtgcctttaa
    10921 aaatataaaa cctttccggt tatgaaggtc aagatgcgaa ttggagtaaa aatgttactc
    10981 tttgtcatgt caacctcggt gctggtgatg ctggttattg ggcttttcat tggttttcga
    11041 ataaataaaa ttgcccggga cgatgcggta agaatagttc aatccgaggc tcagaagacc
    11101 gccagccagg taaaatcgga gctggagctg gacatgggct tttccagggc gctggcacat
    11161 gccctgtaca tctaccccaa attcgatacg cttactctcg actccatatt cattaacata
    11221 ctcaaaaatc aggtagccaa caacccacgc tacctcactg tttggtactc tattgagtac
    11281 tccgcctacc gaccgggtta cacaaagaat tacggcaggc gttcggttac cgcctatctt
    11341 agggatggta atattggcgt tgatatcgag cataagaacg ttaccggtga tgttacaaca
    11401 agcaactact actcctctaa aacatgcaac tgtgaaatgc tgctcgaccc ctataccttt
    11461 gtttatgggg gcagggaggt gctggccacc agccttagcg tgccggtaag ggtaggcgat
    11521 cggtttgccg gtttaggagg tgtagatatc tcgttggaga agtttcagga aaccatcgaa
    11581 aaaattaaac cctacagcaa caccagggta agtctgattg ccagtgacgg gaccattatt
    11641 gccgatacga accccgaaaa cgccaggcta aagttcagcg aacttttccc ttccgaagag
    11701 gcaacctacc agctttccca aaaaataaaa aatgggttgg actttcagta cctcacaatt
    11761 caggacaaca gagaattttt aaatattata acccctgtaa aagttggaga ttcgcctacc
    11821 tactggtcgg tttgtctttc cattcccctc gatgtagtgg ttgccgaggc tagaaaagct
    11881 gttatttata caattctggt attccttttt ggaatcattc tgcaaaccct ggccatttgg
    11941 tatatttcaa actacataac ccgcccgata aagaaaacta ccggggtgct gaacaacctt
    12001 gcagaaggcg atattgatat caacagcaaa atatacattc atacgggtga cgagcttgag
    12061 gaaatggcac gttccaccag caagctgatt gatgggctca accatacagt tcagtttgcc
    12121 cgagaaattg gtcagggcaa gcttgatagc gaatttaccc tgcttagcag taaggacagg
    12181 cttggggaag ctcttattga tatgcaaaaa agcctgctta aagccaggga agcagaagtg
    12241 cagaggaagg atgaggaaaa taagcaaaac tgggcaacca ggggtgttgc catgtttggt
    12301 gaggtgcttc ggcagcataa cgataacctg aacgagctgt cctacctcat cgtcaaaaac
    12361 ctggtgagct ataccggttc cattcagggc ggcatatacc tgataaatga caacgactcc
    12421 gataacccgt tcctcgaaat gaccgcctgc tatgcctacg accgtagaaa atttatggaa
    12481 aaaaacattc tgcccaacga gggattggtg ggcaggtgct acgttgaggg aaaaacaatt
    12541 ttgatgaccg atgttcccaa gaactacatt aaaataacct cagggctggg cggcgagaac
    12601 ccccgatgcc tcctaattgt ccccttaaag gtaaacgaca cggtgttggg ggttatagag
    12661 ttggcctcat tcaaccctta tcagaagtac caggttgatt ttatcgaaaa aatagccgaa
    12721 agcatagctg ccacgctgtc gagtgttagg gtgaacatcc gtaccgccga gctgctggaa
    12781 cgtacacaac agcaggcaga ggaaatgaaa gcgcaagagg aggaaatgcg gcaaaacatg
    12841 gaggagctac acgccaccca ggaggagatg gagcgtaaac gacttgagca ggaatccatt
    12901 aatacccagc ttgctgagga tagaacaatc ctcaacagcc tgatgcagaa tttacccgac
    12961 tgcatgttcc aaaaggatgt taacggccgc tacgttaggc taagcaactc catgctcaac
    13021 cttttaggaa tcgaccagcc cgacgaggtt atagggttaa ctgattttga cctgtttcct
    13081 acttcgctgg cgaagctact ttccaagctg gacagcgaag tgctgagctc tcgtcaacct
    13141 attaccagta aacccattga gctggtgctt aagaatggag tagaacagga tgctctactc
    13201 acgcttatgc ccctggttga agagaatgga gatgctattg gagttatggg cgtcatgaaa
    13261 cttgcttaag cggaataaag cggctcgagg aattgagcat caactccagc acctgtaaag
    13321 ctaggtgaac acctcataca caaaaagagg catcaaggaa at
//
LOCUS       DAOPHX010000066        13326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_5436_length_13326_cov_4.727978, whole genome shotgun sequence.
ACCESSION   DAOPHX010000066 DAOPHX010000000
VERSION     DAOPHX010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 13326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13326
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5436_length_13326_cov_4.727978"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1792
                     /locus_tag="PK502_06280"
     CDS             <1..1792
                     /locus_tag="PK502_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HPH00457.1"
                     /translation="ETLFHEKQTIIENCLFGVDINPNSVKICRLRLWIELLKNAYYKE
                     SGELETLPNIDINIKCGNSLISRYALDADIKAALKRSKWSIDSYRIAVMSYRNAQTKD
                     EKRAMERLIDQIKSDFVGEIAANDKRLIKLSKAKGELLTLTTQTTLFDRTKAEKAAWE
                     KQVKALTETVKNLETELEEIRNSKIYENAFEWRFEFPEVLNNEGDFVGFDVVIGNPPW
                     GASLSKEQKDFLKIRFDKIDSSTPNTYAYFIGLSFKISHSFITQILPDSILVKDFEKT
                     RCLCMNGLYNLSWYQNTAIPDTFRPFIDVEHDVCILFIDKINSSEKSKIYTRFYNNSE
                     LIENERISHKTNFILKEYDSTYNILLTTEDICILNKLRGFQTLDNFLQCHEGIHSGNI
                     REKLFLRTKTTGDEKSMFLGAKNGDRIENYISFVSGWYVDYRESIIDKAKGEYASLRD
                     VNIFNKPKIYLTRTGNPFKAFIDEYNYASNNFFSIQFKNYKLNSIDNLLPILGLLNSK
                     FANYYIRRIIAPSLGNTFVETKIIHLLKLPFSPMLFESIELKKIMSDLIAIKTNNRDS
                     DITHLVFKIDQLVYQLYDLTEDEIKIIESC"
     gene            2001..2705
                     /locus_tag="PK502_06285"
     CDS             2001..2705
                     /locus_tag="PK502_06285"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HPH00458.1"
                     /translation="MAKYNQVILQGNEWVKIDNEKYLLSLKERDRQAIKLIKQLYGAK
                     TSNELIRITYIKYPYLAINSTVAKDKLSSDEFDKVLQAKPKSDKTILFTIGYEGLSLE
                     EYLNKLILSDIKVLCDVRRNPLSMKFGFSKSQLQKACQGVGIEYVHIPELGIESDKRQ
                     VLNTQSDYDKLFSLYRSEMLTQTLDYQLKLITLLKEKKRIALTCFEANINQCHRKHLA
                     DAIVNLSDFNYELKHI"
     gene            2707..3534
                     /locus_tag="PK502_06290"
     CDS             2707..3534
                     /locus_tag="PK502_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007481929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00459.1"
                     /translation="MAKTKVLIAVKTYPTLASKYDELVCTAGFREDGTWVRIYPIPFR
                     KLDYDKQYKKYDWVEIDLVRNKSDFRPESFRPYSIDESISIVGHLNTDNNWLLRKKIV
                     LKNVYTNLDILIADAKDKNKYTSLAVFKPTSIHDFIFEPIEREWDKSKLDKLKAKALQ
                     LNLFKNAENPFEVVKKLPYKFSFVFSDDSGKRSTLMIEDWETGQLYWNCLKKHEGNEQ
                     KACADVRQKYFDDFSKTKDLYFYLGTTREFHLIAPNPFIIIGTFHPKKDAQLSLFNI"
     gene            3717..5084
                     /locus_tag="PK502_06295"
     CDS             3717..5084
                     /locus_tag="PK502_06295"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HPH00460.1"
                     /translation="MKKFLAFLIILLCLVQQYSVGVNTPVSCFPPVSVAGSDFYTYFA
                     DRTMRIDYRHSGDDKTESFEMAKIASEGSWAGRTNHLNDPWKLGLYFFEVKDAASGKT
                     IYSQGFCSVFGEWQTTAEAKAGVKTFNETIRLPWPKNEVNVVLQKRDSLNRLVPVWNL
                     TVNPVEYEDLIPTRPSAFEPKVLLDNGDPKFKADIVILGDGYAENEDSLFWKDAQRFS
                     DAFFSVEPFKSRIADFNVRAVLGKSTQSGIRLQRKDFYPDNLLKTSYYAFGIERYVLA
                     SDDWIVKDYASIVPYDFIVILMNSDKYGGGGIFNLYITAAAHSRASEYVMVHEMGHHI
                     AGLADEYYTSDVAYEPANTKVEPWEFNITALINPDSLKWRDLVAPGTPIPTPWNKVEY
                     EQGGGNKVLLSDPAAGKVGAYEGANYLSKGMFRPEVNCIMIAGKEFCQVCKRGINRVL
                     DLYCE"
     gene            5331..6359
                     /locus_tag="PK502_06300"
     CDS             5331..6359
                     /locus_tag="PK502_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPH00461.1"
                     /translation="MDSNVLVSLLVILLPLSFISERITNLVKLFLPTSNFLSIGNVRY
                     RENNPLLEKKRERRIFLISLLSGELVAFSLKADLFTILNSGKFGWDEPASGFSWIIGC
                     LFTGFFLSWGSKFWHDLLGIILEAKNMRRAIIDEKNLDVQLRAFGTQETSSPSEEVAA
                     VTIKPNQPQAAKPEFVQDKPTLQRIAKLHPLLREEAKEIYLQILAQGIAVRFTDTLRT
                     FAEQDELYAKGRTKPGKIVTQAKAGQSFHNYGLALDFCLLLADGREASWDRHTDINLN
                     LRYDWDEVVALFRHYGWEWGGDWVNFKDYPHFQKTFGLSTAQLLKLYNRKEVDGEGYV
                     VIKENEFS"
     gene            6467..7795
                     /locus_tag="PK502_06305"
     CDS             6467..7795
                     /locus_tag="PK502_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00462.1"
                     /translation="MGKRKVFLFIAAALLAIWVVGVVRDFDKLCKKTPNPEYQNFITQ
                     QKQNLQRTVEQLTTGEVIRVDGANLYRSDNCIGSNFEGAEVKLAGIQLYDSIYGSKSV
                     KPYYTYSCNGAILDPDHPLFGLPDIEVLIGPVTYYEPELSGVKALMNFGFSRDPKPHK
                     TFRKALYDSTGKKLQVFEMQLWLTYFKVAISVISNRDKPQITPDDESLNARIYPGKWY
                     CSSKPLISVNDLEQKEEYKNHQYGDITFVLEVNPNASPWYINTGSVHTARPDIAVGAV
                     ICKELVKRPEDENMMHLQTQRGMLTPLYFKPFERTVQPEKIDSIAGQVETEIQDYKNF
                     DIWNKPYYARLYSRNIGSRQKGFFGQKKFDEQVEYTFLLPLLVVGSWDVQLPWDVMPE
                     MQAPVPYYRGFNLKNLFPEWGIKSGGWISAGLLVAIVVLLLLWFLPMLRR"
     gene            complement(7792..8571)
                     /locus_tag="PK502_06310"
     CDS             complement(7792..8571)
                     /locus_tag="PK502_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPH00463.1"
                     /translation="MLTRRNIQRIVSLRQKKHRNELGLFVAEGQRLVSELLKSHLDVA
                     EIYHTGNLEKMFTVDSKIATTLVSENEMNRISALSTPTPVLALVKMPLYNAEPINHAN
                     QLVLALDSIQDPGNMGTILRLADWFGIDTILCSNDTADIFSPKVVQASMGAIARIKVH
                     YCNLPTKLSAYSKNSPIFGTYLEGESIYDLPGVESGCIVMGNEGNGISPEVGSCITQK
                     IHIPDFAGGRARVESLNVAMATAIVLSEFRRKQVALINHET"
     gene            8816..11194
                     /locus_tag="PK502_06315"
     CDS             8816..11194
                     /locus_tag="PK502_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPH00464.1"
                     /translation="MRIFSRIRKEFIAYRLIPVMLGVVFVSGCSITRNVPEDGYLLNK
                     SNIKTDSKEISTRDMEDYLKLKPNKKILGFRFHLRLYNMANPEKNKGVNKWLKNIGEE
                     PVIMDTNLIAEGSRNLLNYLQSKGFYNAKVEDSIYYHKKKANVIYSVYPNVPYRIKSL
                     SYLVEDSAVLELVKKDTANCLIGVGKRFDTDMLQQERTRIEVFLKNNGYYSFSKDYIT
                     FTADTNFRSHSVNLILTIRNPFTIDEKGNKVKSNFKKFKVKRVFIYPNFEPLEYLTYQ
                     QQATLDTVYYKDIQFIFSGDAGVNLAVIYENILIRPGLTYSSEAVKKSQANLSSIRLF
                     KFVNISFKEAKEAEFPISPFDFLDEDSASSDSTEYAYLDCYIQLTQNTLQSYQVELVG
                     TNTTGSLGAEANLSYQHKNLFRGAEVFDFKLRGLVETAQQKINFDNTLELGGSMGLSI
                     PKYIGPFSSRVQVSRSAPKTQVTASYSYQHRPDYTRTIASLLFGYSWRSGSFITHNVN
                     PAEVNAITISRISDDFKAYIDTTSLKYSYISQIVTVSSYGFTFNNQSPQKPGSYTYLR
                     YNFELSGNLLNLAFAAFNQPKNADGSYSIFNTNFSQFVRSDLNYTYNQYVDANNSFAY
                     RIFLGVGYPYGNSKALPFEKRYFTGGANGIRAWQARALGPGSYYQAKERYPNRTADIK
                     LEANLEYRYKLFWKVEGALFIDAGNIWSMPGIDDRKGATFHFDKFYEQLALGSGLGFR
                     MNLGFLILRTDFGYKIFDPAINPGKPFRPWVPLQQKFMWRDITFNFGIGYPF"
     gene            11255..12313
                     /locus_tag="PK502_06320"
     CDS             11255..12313
                     /locus_tag="PK502_06320"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HPH00465.1"
                     /translation="MSYNRIVELLGDKSEFLLNHKCETVKKEQLHLPGPDFIERVVAL
                     SNRSPQVLRNLQEMFNHGRLAGTGYLSILPVDQGIEHSAGASFAPNPIYFDPENIVRL
                     AIEGGANAVASTFGVLASVSRKYAHRIPFIVKLNHNELLTYPNKYDQIMFGSVEEAWN
                     LGAVAVGATIYFGSEESTRQIIEVAQAFERAHELGMVTVLWCYLRNNAFKKDGVDYHV
                     AADLTGQANHLGVTLQADIIKQKAPENNGGFKAIGFGKTSDKVYSQLSSDHPIDLTRY
                     QVVNCYNGRAGLINSGGASSGSSDLAEAVFTAVVNKRAGGMGLISGRKAFQRPMDEGV
                     TILNAIQDVYLESKIDIA"
     gene            12556..>13326
                     /locus_tag="PK502_06325"
     CDS             12556..>13326
                     /locus_tag="PK502_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPH00466.1"
                     /translation="MNKFKSKLKDYFSFTRSERNGTLVLSFLLVVFMLAPAAIRHIKF
                     SRNSPSSTMNTAIDSFFLSARFNPRNEVEKTTFSIAHEEVPRRAEPKQFPFDPNTISL
                     DSLVELGLSVKQAGVILKYREKGGHFDSATDLAKIYVLDSSTLARLKPLVRITAKSHE
                     RQLLNAESGSSSIFPVELNTADTTQLMRIKGIGRSYANRIVTFRNLVGGFYSVNQLAD
                     IYGITPELVASISPQVKVDSLAIRRINLNEVTYEELRSH"
BASE COUNT         3993 a         2756 c         2823 g         3754 t
ORIGIN      
        1 agaaacactt ttccacgaaa aacaaaccat cattgaaaac tgcctttttg gtgtggacat
       61 aaaccccaac tcggttaaga tttgccgttt gcgcctttgg attgagctgc ttaaaaacgc
      121 atactacaag gaatcgggcg agctggaaac gctccccaat attgatatta acattaagtg
      181 cggtaactcg ctcattagcc gttacgccct cgatgccgat attaaagccg cccttaagcg
      241 cagcaagtgg agcatagata gctaccgcat tgccgtgatg tcctaccgca atgcgcaaac
      301 caaggacgag aaaagggcaa tggaacgcct gattgaccaa attaagagtg atttcgtagg
      361 cgaaattgcc gcaaacgata aacgactgat taaacttagc aaggcaaaag gcgaattgct
      421 cacacttacc acccaaacaa cgcttttcga ccgcacaaag gccgaaaagg cagcgtggga
      481 aaaacaggta aaagccctta ccgaaacagt aaagaacctg gaaaccgagc tggaggaaat
      541 ccgcaacagc aagatttacg agaatgcctt tgagtggcgt ttcgagttcc ccgaagtatt
      601 aaataacgag ggcgattttg tggggtttga tgtggtgatt ggaaatccgc catggggagc
      661 ttcattatcg aaagaacaaa aagacttttt gaaaataagg tttgataaaa ttgatagttc
      721 aactccaaac acctatgcct attttatagg tctctctttt aaaattagtc attcttttat
      781 tactcaaatt ttacctgaca gtattcttgt taaagatttt gaaaaaacta gatgtttgtg
      841 catgaatggc ttgtataatt tatcttggta tcaaaacact gcaatcccag atacttttag
      901 accatttata gatgtagaac atgatgtttg tatattgttt attgataaaa taaattcgtc
      961 agaaaaatca aaaatctata caagatttta taacaactct gagttaatag aaaatgagag
     1021 aatttcacat aagacaaact tcattctaaa ggaatatgac agcacatata atattctgtt
     1081 aactactgaa gacatatgta ttctaaacaa acttagagga tttcaaacat tagataactt
     1141 tctccaatgt catgaaggaa ttcattcagg caatataaga gaaaaacttt ttttaagaac
     1201 aaaaactact ggtgatgaaa aatcaatgtt tctgggagca aaaaatggag acagaataga
     1261 aaattacata tcttttgttt ctggttggta tgttgattac agagaaagta ttattgacaa
     1321 agccaaggga gaatacgcat ctttaagaga tgtaaatatt tttaataaac caaaaatata
     1381 tttaacaaga actgggaatc catttaaagc atttattgat gaatacaatt atgcatctaa
     1441 taacttcttt tccattcaat tcaaaaacta caaattgaat agtatagata atcttttgcc
     1501 tatacttggt ttacttaatt caaaatttgc aaactactat attagaagaa tcattgcacc
     1561 atcgctaggc aatacctttg tcgaaacaaa aattattcac cttctcaaat taccttttag
     1621 ccctatgctg tttgagtcta ttgaattaaa aaaaattatg tctgatttaa ttgctattaa
     1681 aacgaataac cgagattcag atatcacgca tttagtattt aaaatcgacc aactcgttta
     1741 ccaactttat gacctaaccg aagacgaaat aaagattata gagagttgct agctgctaat
     1801 aaaaaaacta attttgccgc atgtactacc gccgcaaaat attactagga ttgctcgaag
     1861 cctttgacaa taggctcgaa aagatacagc ttcagaaact gctaatgctt gttacaaagc
     1921 aacagcaaaa gcctgacttc cattttgttc cctacaagta tggctgttat tcctttcagg
     1981 ccaactctga ccttggcaca atggcaaagt acaatcaggt tatactacag ggtaacgagt
     2041 gggttaaaat tgataatgaa aaatacttat taagcctaaa agagcgagat aggcaagcaa
     2101 ttaagttaat caagcagcta tacggtgcaa aaacatccaa tgagctaatt aggataacct
     2161 acataaaata cccatacttg gccattaata gcactgtagc aaaggataaa ttaagttccg
     2221 atgaattcga caaagtttta caagcaaagc caaaatccga caaaacaatt ctattcacta
     2281 tcggttacga gggcttaagc cttgaagagt acctgaacaa attaatacta agcgacatta
     2341 aagtgctttg cgatgttcgg cgtaatccgc taagcatgaa atttggcttt agcaaatcgc
     2401 agttacaaaa agcctgtcaa ggagtaggaa ttgaatatgt ccacattcca gagttaggca
     2461 ttgagtccga taaacgccaa gtactgaata cccaatccga ttacgataag ctcttttcgt
     2521 tgtaccgttc cgaaatgtta acgcaaacgt tggattatca attaaaactg ataactttac
     2581 ttaaagagaa gaaacgtatt gcgctaacct gctttgaggc caacataaat cagtgccacc
     2641 gtaaacactt agccgatgca atagtaaatc tttcagactt taattacgaa ctgaagcata
     2701 tttaaaatgg ctaaaacaaa agtacttatt gctgttaaaa cctaccctac gctagcttcc
     2761 aaatatgatg aattagtatg tacagcaggg tttcgtgaag acggaacttg ggtgcgaatt
     2821 tatcctatcc cttttcgcaa actcgattat gataagcaat acaaaaaata tgattgggtt
     2881 gaaatagatt tagtccgtaa caaatctgat ttcagacccg aaagttttcg cccttactcc
     2941 attgacgaat caattagtat tgttggtcat ttaaatacag ataataactg gttacttcgc
     3001 aaaaagattg tattaaaaaa tgtttatacg aatctcgaca tactaattgc agatgcaaaa
     3061 gataaaaaca agtatacttc cttagcagtt ttcaagccta catcaataca tgattttatt
     3121 tttgaaccca ttgaaaggga gtgggacaaa tcaaaacttg acaaattaaa agcaaaagcg
     3181 cttcagctta atctatttaa aaatgctgag aacccttttg aggtggtaaa aaagttacca
     3241 tataaatttt ccttcgtttt ttcagatgat tcaggaaaaa gaagtacttt gatgattgaa
     3301 gattgggaaa ctggtcaact ttattggaat tgcctaaaaa aacacgaggg taatgagcaa
     3361 aaagcatgcg ccgatgttag acaaaagtat tttgatgatt tctctaaaac caaagattta
     3421 tatttctatt taggaacaac cagagaattc catttaatag ccccaaaccc atttataata
     3481 attggcactt ttcatccaaa aaaggatgct caattgagtt tgtttaacat ttaaggcttc
     3541 atcaatgcaa catttttgat ggagagggaa ggttaaggtg atagctctac cagtatctgg
     3601 gcaggaaaaa gttacttttt tccccttttg tcttgaaaaa gtatttattt tacctccctt
     3661 ctatccataa aatgctgcct taaattaata gaacttagtc attaacattt ctaaaaatga
     3721 aaaaatttct tgccttccta atcattttgc tgtgcttggt gcagcaatat tctgttgggg
     3781 ttaacacgcc tgtaagttgt ttccctcctg ttagtgtggc cgggagtgat ttttacacct
     3841 actttgccga ccgaaccatg cggattgact accgccattc aggcgatgac aaaaccgaaa
     3901 gtttcgaaat ggccaaaatt gcttctgaag gtagctgggc aggcagaacc aaccatctta
     3961 acgacccatg gaagcttgga ctttactttt tcgaggtaaa agatgctgcc agtggcaaaa
     4021 ctatctactc gcagggtttc tgcagcgttt ttggcgagtg gcagacaact gccgaagcca
     4081 aggccggtgt taaaaccttt aacgaaacca ttcggttgcc ctggccaaaa aatgaggtta
     4141 acgtggtgct gcagaaaagg gattcgctta accggcttgt gcctgtttgg aacttaacgg
     4201 ttaatccggt tgaatacgaa gatctgatac ccacaaggcc atctgctttt gaacctaaag
     4261 ttttgctgga taatggcgat ccaaaattta aagccgacat tgtcatcctt ggcgatggct
     4321 atgctgaaaa tgaggactcc cttttttgga aggatgcaca gcggttttcc gacgcatttt
     4381 ttagcgttga accttttaaa tcgagaattg ctgattttaa tgtcagggca gtgcttggaa
     4441 agtcaacgca aagcgggatt aggcttcaac gtaaggattt ttatcccgat aacctgctaa
     4501 aaaccagcta ttatgctttt ggaattgagc gctacgtgct cgcatccgac gattggattg
     4561 ttaaggacta cgcctccatt gttccctacg attttatagt aattctcatg aattctgata
     4621 agtatggtgg tgggggcatt ttcaacctgt atattaccgc cgctgcccac tccagagcat
     4681 ccgaatacgt gatggtgcat gagatggggc accacattgc cggcctagcc gacgagtact
     4741 acaccagcga cgtggcttac gagcctgcca acaccaaggt tgaaccctgg gagtttaata
     4801 taacagccct tataaatccc gacagcctga agtggaggga tctcgtagcc cctggtacac
     4861 ctataccaac tccatggaac aaggtggagt atgagcaggg tggcggtaac aaagtgctgc
     4921 tttctgaccc tgccgccgga aaggtagggg catacgaggg ggcaaactac cttagcaagg
     4981 gaatgttccg tccggaggtc aactgcatca tgattgccgg aaaggaattt tgccaggttt
     5041 gtaaacgagg gattaacagg gttttggacc tttattgtga gtaggcagct gtctgaactg
     5101 ctacaagcct actactataa aaatacccat tcttttttac cagcatggtt tttctgctgt
     5161 atggtcggta attaagggca ttcaatacct attctaccaa aatatacatt aatatttttt
     5221 acctatagat agcagggtag aataacttta tggttttcta aaaaattcag ctgaaaaatt
     5281 tttcattcaa aaaattttgt aacttccatc ggtaaaataa cttatgagaa atggattcca
     5341 acgttctggt ttcgttgttg gtcatcctgc ttccattgag ttttatctcc gagcggataa
     5401 ccaacctggt aaaactattc ctgccaacct ccaacttttt gtcaattggg aatgtacgct
     5461 acagggagaa caacccccta ctggagaaaa agcgtgaacg cagaatattc ctgatcagcc
     5521 ttttatcggg cgaattggtt gccttctcac tcaaggcaga cctttttact attctgaata
     5581 gcggaaaatt tgggtgggac gagcctgcat cgggcttttc gtggattatt ggctgcctgt
     5641 ttaccggctt ctttctgagc tgggggagca aattctggca cgatttgctg ggtattatcc
     5701 tagaggccaa aaacatgcgt agggctatta ttgacgagaa aaaccttgat gtacagctta
     5761 gggcttttgg tacacaagaa actagcagtc catctgagga agtggctgct gttacgatca
     5821 agcctaatca accacaagca gctaaaccag aattcgtgca ggataagcct acacttcagc
     5881 gaattgccaa gctgcatccc ctgcttcgcg aggaggccaa ggagatttat ttgcagatac
     5941 ttgcccaggg cattgccgtt cgcttcaccg atacgctgag gacctttgcc gaacaggatg
     6001 agctgtatgc taagggaaga accaaacccg gaaaaatagt tacccaggcc aaagccggac
     6061 aatcgtttca taactatggg cttgcccttg atttttgcct attgttggct gacggaaggg
     6121 aggcttcgtg ggacaggcac accgatatca acctgaattt aagatacgac tgggacgagg
     6181 tggttgctct tttcaggcat tatggatggg aatggggagg cgactgggtt aactttaagg
     6241 attatcccca ttttcagaaa actttcgggc tatcaaccgc ccagctgctg aagctatata
     6301 accgtaagga ggttgatgga gaggggtatg tcgttattaa agagaatgaa tttagctagc
     6361 tggcgttaat aatttttctt aatgtagaaa cttgcagctc cgattcatac tgttaccgtc
     6421 atacttttta caaaagagct tcaggaaacc ataatatcat agtaaaatgg gcaaaagaaa
     6481 agtatttctt ttcattgctg ctgctctact ggctatttgg gttgtaggag ttgttagaga
     6541 tttcgataag ctttgcaaga aaactcccaa tcccgaatac cagaacttta ttacacagca
     6601 aaagcagaat ttacaaagga cagttgagca gctgacaacc ggtgaggtaa ttagagttga
     6661 tggcgccaac ttgtatcgaa gcgacaactg cattggctct aactttgaag gggctgaggt
     6721 gaagctggct ggcattcagc tatacgacag catatatggc agtaagtctg ttaaacccta
     6781 ctatacttac agctgcaacg gcgccattct cgatcccgat catccccttt tcgggctacc
     6841 tgatatagag gtgcttattg gtccggtaac ctactacgaa ccggaacttt cgggagtgaa
     6901 agcccttatg aactttggct ttagcaggga tcctaaacca cataagacct tccgtaaagc
     6961 tctttacgat agcacaggta agaagctaca ggtttttgag atgcagcttt ggctaaccta
     7021 tttcaaggtt gctatcagcg ttatatccaa ccgcgataaa ccgcagatca ccccagacga
     7081 tgagtcgctg aatgcccgaa tatacccggg gaaatggtac tgtagctcaa aacctttgat
     7141 atcggttaac gatctggagc aaaaggaaga gtacaaaaac catcagtatg gcgatataac
     7201 ctttgtgcta gaggtcaacc ccaacgcttc gccctggtac attaataccg gaagcgttca
     7261 tactgccaga cccgacattg ctgttggggc agttatctgt aaggagctgg ttaagcgtcc
     7321 ggaggatgag aacatgatgc accttcaaac ccagcgggga atgcttacac ccctctactt
     7381 caaacccttt gagcgtaccg ttcaacccga gaaaattgat agcattgccg gccaggttga
     7441 aacggagatt caggactata agaatttcga tatttggaac aaaccgtact acgccagact
     7501 gtactcacga aatatcggct cccgacagaa aggttttttc ggacagaaaa aatttgacga
     7561 acaggtagaa tacacattct tgctgccatt gctcgttgtt ggaagttggg atgttcagct
     7621 cccgtgggat gtaatgcccg aaatgcaagc acctgttccc tattacaggg gttttaactt
     7681 gaaaaatctt tttccggaat ggggtataaa atctggtggc tggataagtg ccggtttact
     7741 tgttgctatc gtcgtactgc tgctgctttg gtttttgcct atgctccgac gttaggtttc
     7801 atgatttatc aaggcaactt gctttcgcct gaactccgaa agcactatgg cggttgccat
     7861 agctacattt aacgattcaa ccctggctct gcctccagca aaatcgggaa tatgaatttt
     7921 ttgggtgatg cagctcccaa cttcggggct tattccgttg ccttcatttc ccattacaat
     7981 acaaccgctc tccacccctg gtaaatcgta aatgctttca ccctcaaggt aggtgccaaa
     8041 aattggactg tttttactgt aagccgacag ttttgttggt aaattgcagt agtgaacctt
     8101 tatgcgggca atagcgccca tacttgcctg aactacctta ggagagaaaa tatctgccgt
     8161 atcgttggag catagaatgg tgtcaatccc aaaccagtcg gccaaacgaa gaatggttcc
     8221 catgtttccc ggatcctgaa tggagtcgag ggcaagtaca agctggtttg catggttaat
     8281 gggctctgcg ttgtacaggg gcatttttac cagagcaaga acaggcgttg gagtgctaag
     8341 tgcgctgata cgattcatct cattttccga taccagtgta gtagcaattt ttgaatctac
     8401 cgtaaacatt ttttcaagat tcccggtatg gtagatttca gctacatcaa gatgagattt
     8461 cagaagttca gaaaccagcc gttgcccctc ggcaacaaat agtcccagct cattgcggtg
     8521 ttttttttgt cgtaacgata cgattcgttg aatatttctc ctggtgagca taggtacgat
     8581 ttttcgctgt aaatatagat ataattttat cccgaaaagc tgtaaagtgt tgtatgtaag
     8641 agtactttat ggagtattcg gctcatgtac tctacgaagt ttattcagca caaaacatta
     8701 accgctggtc aaacaaagtt cgcttgtgct gtaaaattat gctaattgcc atgtattgta
     8761 aaaacgattt tataaatttc cgagccattt atagtcagtt agaggaatgc attttttgag
     8821 gattttcagt aggatacgta aagaatttat agcttacagg cttatacctg taatgctggg
     8881 agttgttttt gtatccggtt gcagcataac acgaaatgtt cccgaggacg ggtatttgct
     8941 gaacaaatcc aatataaaga ccgacagcaa ggagatttca acccgcgaca tggaggatta
     9001 cctaaagtta aagcccaaca agaaaatttt aggttttcgc tttcacttac gcctatacaa
     9061 catggcaaat cctgaaaaga ataagggtgt aaacaagtgg cttaagaata tcggggaaga
     9121 gcccgtaatc atggatacta accttatagc tgagggttcg agaaatctcc taaactacct
     9181 tcaaagcaag ggattctata atgcgaaggt ggaagattca atatactacc ataaaaaaaa
     9241 ggcaaacgta atctactccg tttaccccaa cgttccctac cgcataaaat ccctaagcta
     9301 cctggttgaa gattcagcag ttcttgagtt ggtaaagaaa gacactgcca attgccttat
     9361 aggtgttggg aaacgcttcg ataccgatat gcttcagcag gagagaacga gaatagaagt
     9421 ttttctgaaa aataatggat actacagctt tagcaaggac tatattacgt tcactgctga
     9481 tactaatttc cgtagccaca gcgtaaacct tatcctcacc attcgtaacc cttttacaat
     9541 tgatgaaaag gggaataagg ttaagtcgaa ttttaaaaag tttaaagtta agcgggtgtt
     9601 tatttatcca aattttgagc ccctagaata cttaacgtat caacaacagg caacgcttga
     9661 tacggtttac tacaaggaca tacagtttat tttttcgggt gatgccggag ttaacctggc
     9721 tgttatttac gaaaatattc ttattcgacc cggcttaacc tattccagcg aggcagtcaa
     9781 aaaatcacaa gccaacctta gctccataag gctatttaag tttgtaaaca tttcgtttaa
     9841 agaggctaag gaagccgaat ttcctattag ccctttcgat tttttggatg aggacagtgc
     9901 cagctccgat tccaccgagt atgcctatct cgattgctac attcaactta cccagaatac
     9961 gctgcaaagc taccaggttg aacttgtggg aactaacacc accggttcgc tcggtgccga
    10021 agctaacctt tcctaccagc acaaaaacct attcaggggt gccgaggtgt ttgattttaa
    10081 gcttaggggc ctggttgaaa cagcccagca aaagataaat tttgataaca ccctagaact
    10141 tggcggctcc atggggctga gcatacccaa gtatattggc cctttttctt ccagggttca
    10201 ggttagccgg tcggctccca aaacccaggt aaccgcatcc tacagctacc agcatcgccc
    10261 cgattatact cgaaccattg ccagcttgct gtttggctat agctggagaa gcggaagctt
    10321 tattacccac aacgtcaacc cagccgaggt gaatgccatt accataagcc gtattagtga
    10381 tgattttaag gcctacattg atacaacttc gctcaagtat agctatatca gtcagattgt
    10441 taccgtgtcg agttacggct ttacctttaa caaccagagt ccccagaagc ccggtagcta
    10501 cacctacctt cgttacaact ttgaactttc gggtaacctt cttaaccttg cctttgcagc
    10561 ctttaatcaa cctaaaaatg ccgatggttc ttacagtata ttcaatacaa atttttcaca
    10621 atttgtccgg agcgatttaa actataccta caaccagtat gtagatgcaa ataactcgtt
    10681 tgcgtataga atttttcttg gcgtaggcta cccctatggc aactcaaaag cattaccatt
    10741 cgaaaaacgg tacttcaccg ggggcgcaaa tggaataagg gcgtggcagg caagagcact
    10801 aggacctgga tcatactatc aggcaaaaga acgctaccct aaccgtacgg ccgatatcaa
    10861 gctggaggct aacttagaat acaggtataa gctcttctgg aaagtagagg gtgccttgtt
    10921 catagatgcc ggcaacatct ggtctatgcc cggaattgac gacaggaaag gtgcaacatt
    10981 tcatttcgat aagttttacg aacagctggc tttgggtagc gggttgggat ttaggatgaa
    11041 tctaggtttc ctgattctta gaaccgattt tggctataaa attttcgacc cggcaatcaa
    11101 ccctggtaaa ccatttaggc catgggttcc cctgcagcaa aaatttatgt ggagggatat
    11161 taccttcaac tttggtattg gctatccttt ctaagatttt gttttttacc ctatttttat
    11221 atagttttgt ataaaccatt ataatctcaa tgccatgagt tacaatagaa ttgttgagtt
    11281 gctcggcgat aagtccgaat ttcttctaaa ccacaagtgt gaaacggtga agaaggaaca
    11341 gctgcacttg cccggtcccg attttattga aagggttgtt gcactatcta accggtcacc
    11401 tcaggtttta cggaaccttc aggaaatgtt taaccatgga agacttgctg gcacaggtta
    11461 cctgtccatt ttgccggttg accagggtat tgaacatagc gctggtgcat cttttgcccc
    11521 aaatccaata tattttgatc cggaaaatat tgtaaggctt gctattgagg gtggtgcaaa
    11581 tgcagttgcc tctacttttg gagttttagc atcggtttcc cgaaagtatg cccaccgcat
    11641 ccccttcatt gttaagctta accataacga actgcttacc tatccaaaca agtacgatca
    11701 aatcatgttt ggctcggtcg aagaggcatg gaaccttggt gctgttgccg tgggggcaac
    11761 aatctacttt ggctcggaag aaagcacccg acagattatt gaagtggctc aagcctttga
    11821 gcgtgctcac gagctaggta tggttaccgt cctttggtgc taccttcgca acaatgcgtt
    11881 taaaaaggac ggggtggact accatgttgc tgccgacctt accggccagg caaaccatct
    11941 gggagttaca ctacaggctg acatcataaa gcagaaagca cctgaaaaca atggggggtt
    12001 caaggctatt ggatttggaa aaaccagcga taaggtttac agccagcttt catccgatca
    12061 tcccatcgac ctaactcgtt atcaggttgt taactgctac aatggacgtg ctggccttat
    12121 caactcggga ggtgcttcga gcggttcgag cgatctcgca gaggccgtat ttacagctgt
    12181 agtcaataaa cgggccgggg gtatgggctt aatcagcggc agaaaagctt tccagcgccc
    12241 tatggatgaa ggtgttacaa tactaaatgc tattcaggac gtttaccttg aaagcaagat
    12301 tgatatagcg taaatcaact tcatattttc tgtaaaccca caactttgtt gttgtgggtt
    12361 ttttgctaat acccgagcaa acatttgcat ttattctgct aattttataa aattgtttac
    12421 cagaagctta agcttccgat tttgagaatg catttatccg tgttgattta ttgtaaatat
    12481 tgttttctgg ctgcattact ttaagctggt tctggttggg ttgattgctc ctttatacat
    12541 agccggcagg ttgccatgaa taagtttaaa tcaaaattga aagactactt ttcatttacc
    12601 cggagcgagc gcaacggaac gctagtacta tcatttctgc ttgtggtttt tatgcttgct
    12661 cccgcagcaa tcaggcatat aaagtttagc aggaatagcc cgagtagcac tatgaatacc
    12721 gctattgaca gctttttcct ttcagcccgg tttaacccta gaaatgaggt tgaaaaaaca
    12781 actttttcta ttgcgcacga ggaggttcca cgacgagctg aacccaaaca gtttcctttt
    12841 gaccccaata caatttcact ggatagcctg gtggagctcg ggttatcggt aaagcaggct
    12901 ggggtgattt taaagtaccg tgaaaagggt gggcacttcg attcggccac cgacctcgct
    12961 aagatctacg tgctcgattc ctcaacgctg gccaggctga aaccgcttgt caggattact
    13021 gccaaatcgc atgaacggca actcttgaac gctgaaagtg gatcaagcag tattttccct
    13081 gtggagctga atactgcaga caccacccag ctaatgcgga taaaaggtat aggcagaagc
    13141 tatgccaacc gaattgttac ttttcgaaat ctggttggag gattctacag tgttaaccag
    13201 ctagctgata tttacgggat aacccccgag ctggtagcct caatttcgcc ccaggtaaag
    13261 gttgacagcc tggccatcag aagaataaac cttaacgagg taacctacga ggagttgagg
    13321 agccac
//
LOCUS       DAOPHX010000067        13217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_5492_length_13217_cov_6.128552, whole genome shotgun sequence.
ACCESSION   DAOPHX010000067 DAOPHX010000000
VERSION     DAOPHX010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 13217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13217
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5492_length_13217_cov_6.128552"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PK502_06330"
     CDS             complement(<1..655)
                     /locus_tag="PK502_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPH00467.1"
                     /translation="MKRILVGSTLVFALVLICPFIALSQEINVLPKQKQDDVDRYREL
                     VERYNQADNPQQAAFYLNKIAFIYWENGNVNEAIKYFLETVPLNEKIGNYTDVKAVYS
                     NVALIYADMDRLDLTLEYFNKSLDARRKLNDKGELAAGLIDVAYIHTALNQNEKAVQL
                     LDEALALSKEVNNPRLILNCLRLLSQNYDKMGNTAKANEYAGMFAVYEQQLSTQQLKT
                     "
     gene            1070..1933
                     /locus_tag="PK502_06335"
     CDS             1070..1933
                     /locus_tag="PK502_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00468.1"
                     /translation="MRKQVVAAFCLAGLAMASEKLIAQDFEVAPVKLMFDAEPATSQT
                     KTISVKNHSSRRVSYTVAVADFLPSNTGEKKTLPPNSTKRSCANWLNINPSFFDLNPG
                     EDIQVQVTMLVPGEEYGSTWCMLYIQPTTEQTSWTADKQLSTGITVTGRIGVQVYQSP
                     KSNQFSSVKISNLQEITQPGDKLRRFSASVENLGDKVTMCKIFLLASNMVTAEERQFA
                     AQNIEVFPKMSRNIELELPNDLVAGTYALAAIVDYGPKFPLEGTQIIIEVKGENRSIN
                     PLPPDTTSLSK"
     gene            1946..3103
                     /locus_tag="PK502_06340"
     CDS             1946..3103
                     /locus_tag="PK502_06340"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HPH00469.1"
                     /translation="MPGSIKFILAVCLSFMLAPAAAQKTIVFVEKTGQNGLLKEKYQG
                     VVSDGDTIRQGPYVYYSDNGILIQEGYYNAGKPDSIWLTYFPSGARMSLFRYKNGVKD
                     GEFIFWNQEGSVYQKGTYKNNLLHGEVTTYHRNGNVDTRSVYRNGRLNGLTEVFAEDG
                     KIKLRSEYLNDTLNGVWQTYYESGELNYSGSKSKGKYDNVLLAYHKNGSLQRAARYHD
                     GVLDGEVVSYYENGKPQVTSHFSMGKQTGEFVEYYPNGYVRERGMYKENQKEGMWMSF
                     YPNGQLHGKGNFKQGSLIGEWIFFHDNGNLKQRGSYCNGKACGLWQYFNPQGNLILCG
                     VYVDDLQNDIWTTFYPNGSVAGRILFVNNKPAGIRWVYQQNGSMEKRGGQL"
     gene            complement(3192..5168)
                     /gene="gyrB"
                     /locus_tag="PK502_06345"
     CDS             complement(3192..5168)
                     /gene="gyrB"
                     /locus_tag="PK502_06345"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPH00470.1"
                     /translation="MSSEGLKGEELSSNGYSADSIQVLEGLEAVRMRPSMYIGDTSIR
                     GLHHLVYEVVDNSIDEALAGFCNRIDVTINEDGSITVVDNGRGIPVDYHEKEKKSALE
                     VVLTVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSIKLVAEVMRDGKLYRQEFSMGKP
                     VTSLEVVGESPSTGTIITFMPDSQIFTQSISFNFEIIASRLRELAFLNKGIRLTLTDK
                     RELVENGEAHYRHEEFYSVEGLKEFIKFLDGNRESLTEKIIYIESDKGDVPVEVAMQY
                     NTSFSENVHSYVNDINTVEGGTHLTGFRRALTRTLKKYADDSGMLSKLKFDINGDDFR
                     EGLTAVISVKVAEPQFEGQTKTKLGNPEVSLAVDQAVSEALANYLEENPRDARNIVQK
                     VILAAQARHAARKARELVQRKTVLSGSGLPGKLADCSDKDPLKTELFLVEGDSAGGTA
                     KQGRDRSFQAILPLRGKILNVEKAMQHKIFENEEIRNIFTALGVSIGTEEDSKALNLS
                     GLRYNKVIIMTDADVDGSHISTLIMTFFFRYMTDLINEGHLYIATPPLYLVKKGKEDR
                     YCWNEEERLKAVQELGKGRDSSVHVQRYKGLGEMNAEQLWITTMCPDTRFLRQVTIES
                     AAEADRIFSMLMGDEVPPRREFIEAHAKYANIDA"
     gene            5492..6286
                     /locus_tag="PK502_06350"
     CDS             5492..6286
                     /locus_tag="PK502_06350"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPH00471.1"
                     /translation="MTQYLELLEHILKNGVQKTDRTGTGTISVFGYQMRFDLSRGFPL
                     LTTKKLHLKSIIYELLWFLRGDTNIKYLNQNGVTIWDEWANREGELGPIYGYQWRSWP
                     ASDGRHIDQIAQVVGSITQNPDSRRHLVCSWNVGDIGKMALPPCHVLFQFYVAGGKLS
                     CQLYQRSADVFLGVPFNIASYALLTTMVARVTGLEPGEFIHTLGDAHIYLNHVEQVKL
                     QLTRDPRPLPTMHINPQVKSIFDFSYDDFSLENYNPHPHIKGNIAV"
     gene            6413..6580
                     /locus_tag="PK502_06355"
     CDS             6413..6580
                     /locus_tag="PK502_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPH00472.1"
                     /translation="MAYKISDDCTACGTCIDECPVEAISEGDIYKINPDICTDCGSCA
                     DVCPVEAIHPE"
     gene            6706..7566
                     /locus_tag="PK502_06360"
     CDS             6706..7566
                     /locus_tag="PK502_06360"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPH00473.1"
                     /translation="MQIKKRTAQPFRKPLMLAVLAMSATLSFGQPFGVLTYNIRLDTE
                     QDGVNRWDNRKESMVELVNRYSPAIFGVQEALYHQVCYLDSALKKYTYIGVGRDDGKK
                     AGEFSAIFFDNTRLRVIAHSTFWLSEHPDSVSLGWDAACRRVCTYGLFEDMLSHKKFW
                     VFNTHFDHVGKVARKNSAELIVNRINELNGDNLPVIAMGDFNSNPADEPVKILASTLA
                     DGLTCSQKPLNGPVGTFNGFNPEYLPDERIDYIFVLKVRVLTYSHLTDRRENGLLISD
                     HLPVLAEVEI"
     gene            complement(7665..8756)
                     /locus_tag="PK502_06365"
     CDS             complement(7665..8756)
                     /locus_tag="PK502_06365"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPH00474.1"
                     /translation="MSTPRQLNILVCPLGWGLGHASRVVPIVRLLLESGHRVVVAADA
                     NSLALVKSSCPYLETKFFPSYKVQFKRGSSQLLPMLWVAIRLPFINLREHRKLKKLVL
                     ENHIDLVISDNRYGLWLRGIMSVMVTHQLRVIPPRPFKFLTKASEILIRHWLKRFNHV
                     WIPDNEGAGSIAGVLSSPNGLTNIQYIGILSRFSGIAPASNFKTWEMVVMVSGPEPQR
                     ALLVELAANLAHRYSIGCLVVEGKPQNGSELRVVDGIWYAGHLPDEQLAFVLRSCRYL
                     LVRGGYSTIMDLVTLGISGLLIPTPGQTEQEYLAHYLSVKGLFRSVPQQCLPQLDLKE
                     AERRSIPLHSEYSGSQRLKSCVDGVVFRL"
     gene            complement(8760..9533)
                     /locus_tag="PK502_06370"
     CDS             complement(8760..9533)
                     /locus_tag="PK502_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPH00475.1"
                     /translation="MSTFLFDSIVFGPVRSRRLGASLGMNLLPPDRKVCSFNCVYCEC
                     GWTDSIDSDMLPSRGQVQAALEYKLKKMISGGEGLDVITFAGNGEPTLHPDFSGIIDD
                     TIELRNTYFPNADIAVLSNATMLHRPDVVAALKRVDKNILKLDSAFEATINALNQPLI
                     RFDLKNLVDRLMAFEGKLIIQSLFVRGSINGIPVDNTTPAEISAWLKLVDQIRPSEVM
                     IYTIARDTPANDLKKLSEEELNGIAQKVEMLGIATQVSA"
     gene            complement(9825..10376)
                     /locus_tag="PK502_06375"
     CDS             complement(9825..10376)
                     /locus_tag="PK502_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00476.1"
                     /translation="MFVSGSINLSTSMPKTTTGSVTTEGARYTYFRVAPAVGYFLNDK
                     LAVGAGVSYSLQKQKQNDVANLTSMFGITPFVRYYAVSLGDVNIFAQGNVGIEFGANK
                     TKNDGNTTDGPKYLNFGISVMPAISYSLGEKLAIEAFLGSVYYSFNREKDEIAGETHT
                     LKTNGFGLNFDMSSISLGVIYKF"
     gene            10877..13078
                     /locus_tag="PK502_06380"
     CDS             10877..13078
                     /locus_tag="PK502_06380"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPH00477.1"
                     /translation="MERKIYPVEGMSCASCAMTVEKTIKNQPGVEDAGVNFANSTVWV
                     TFDPKAVKPEDLRDSVRSAGYDLIVDAVDVVKVGEEAKQKYADELKLRFWMSAAFSVP
                     LLVLGMFFMDWKLTPWLSAIMAAPVVFWFGRQFFTNGWKQARHFRANMDTLVALSTSI
                     AYLFSLFNTIYPEYWTSRGLQGHIYYESASIIVAFILLGKWLEEKAKNQTTSSIKKIM
                     GLQPDMAWLVEGESLSEVPLSRIRKGDVVLVKPGSRVPVDGRVVDGQSVVDESTISGE
                     SIPVVKKVGDKAWAGTMNQRGSFKLIAEQVGSESVLGRIIATVESAQNSKAPVQHLVD
                     KVAGIFVPAVMLVALLTLITWLVLDPANGFSHGLISMVTVLAIACPCALGLATPTAII
                     VGVGRAAEKNILIRDAESLETAHQVNYIVLDKTGTLSEGVPSVDRIEWFTHETTYPGI
                     LKFIEQNSGHPLADAIVRHFETDDSSGFVADSYESIAGKGVKTTVKGKKYLVGNPDFL
                     RQEGVMFTDTIESSLSEILNNAGSVVGFAENNLLLCLIRVSDRIKPSSREAIKRLKAR
                     NIEVAMLTGDNRANAEFMARELEIAHYAGQQLPSDKADFIKTLKKQGKVVAMVGDGVN
                     DSEALSVADVGIAMGKGSDIAIDVAQVTILNSDLNMLVTLLDISGKTIRTIRQNLFWA
                     FIYNIIGIPIAAGLLYPFYGITLDPMFAGMAMALSSVSVVTNSLRLRTARI"
BASE COUNT         3662 a         3039 c         3031 g         3485 t
ORIGIN      
        1 cggttttaag ctgttgggtg gatagctgct gctcatacac ggcaaacatt ccggcatact
       61 cgtttgcctt agcggtattc cccatcttat cgtagttctg cgaaagcagc ctcaaacagt
      121 tcagaattaa ccgggggttg ttaacttcct tcgaaagggc caaggcttca tcaagcagct
      181 gcacggcctt ttcgttttga ttcaaggctg tgtggatata tgcaacatct atcaaacctg
      241 cagctagttc gcccttatcg ttaagctttc gccgagcatc caggctcttg ttgaagtact
      301 ccagagtcag gtcgagccgg tccatgtcgg cgtatataag agcaacgttg ctgtaaaccg
      361 cctttacatc ggtatagttg ccaatttttt cgttcagggg aaccgtttcg agaaaatact
      421 ttatagcctc gtttacattt ccgttctccc agtaaataaa ggctatctta ttaaggtaaa
      481 aagcagcctg ctgaggatta tcggcttggt tgtaccgctc aaccagctcc ctatacctgt
      541 caacgtcatc ctgcttttgc ttggggagca cgttaatttc ctgcgaaagc gctataaagg
      601 gacatatcaa aaccagtgca aacaccagag ttgaccccac caaaatcctt ttcaatttat
      661 acccagttga ttttaaatca aacgtacaat attttttcaa tacgatacaa aataaaccta
      721 ctttttttat aagaatggaa attatgatga caagcatgtg tatgtagtct ctaaaggtaa
      781 aatagagagc tatgaagctc caccttacaa ggattaccag ccatgtttta agcaagaggc
      841 tagctacaat caccataatg cagggatacg gttaaatcgc catcgaaaca acccgagcaa
      901 gaaaaataaa ataatatctc aacaccttag tcattgatta atcatcattt acatatcaaa
      961 ttttgcctgc tatcaaataa attcatttac ctttgcagaa ttgattagta gttcttcaaa
     1021 aaagcatgat atttaataat tttgttagct taagcattga ccaacaacca tgagaaaaca
     1081 agttgtagca gcgttttgtt tagccggatt agcgatggca tcagaaaagt tgattgcaca
     1141 ggattttgag gtagccccgg taaaattgat gtttgatgcc gagcctgcta ccagccaaac
     1201 caaaacaatc agcgttaaga accacagcag cagaagggtt tcatacacag ttgcagtggc
     1261 cgattttttg ccatcaaaca cgggtgaaaa aaaaacacta cctcccaact ccaccaagcg
     1321 ctcctgcgcc aactggctta acatcaaccc gtcgttcttc gaccttaatc caggagaaga
     1381 tattcaagta caggtaacca tgctggttcc tggcgaagag tatggctcaa catggtgtat
     1441 gctatacatc cagcctacta cagagcaaac atcatggact gccgacaagc agctaagcac
     1501 gggtatcacc gtaacgggta gaattggtgt ccaggtttac caatcgccta agtcgaacca
     1561 gttcagctcg gtaaaaatta gcaacttaca ggagataacc caaccaggcg ataagctgcg
     1621 ccgattttct gcttcagtcg agaacctggg cgacaaggta acgatgtgta agatttttct
     1681 gctggcttcg aacatggtaa ctgccgaaga gagacagttt gccgctcaga atattgaagt
     1741 tttcccaaag atgagccgta acatagagct ggagcttccc aacgacctgg tggccggaac
     1801 atacgccctg gcagcaattg ttgattacgg cccaaaattc ccccttgagg gaactcaaat
     1861 tataattgag gtgaagggtg aaaatcgaag tattaacccg ttaccccccg acaccacgtc
     1921 gctttcaaaa taaaaaatca ccgaattgcc tggcagcatt aaatttattt tggcagtttg
     1981 cctttccttc atgctggctc cagcagcagc acagaaaact atagtgtttg ttgaaaaaac
     2041 agggcaaaat gggctgctta aagaaaaata ccagggggtt gtaagtgatg gtgataccat
     2101 tcgtcaggga ccttacgtgt actatagcga taacggcata ctaatacagg agggttacta
     2161 caacgccggt aagcctgaca gcatctggtt aacatacttt ccgtcgggag caaggatgag
     2221 ccttttccga tataaaaacg gcgttaagga tggtgagttt atcttttgga accaggaagg
     2281 aagcgtttac cagaagggga cttacaaaaa caacctactt cacggagagg ttaccaccta
     2341 ccataggaat ggcaatgttg acacccggtc ggtgtaccgc aatggcaggc taaacggatt
     2401 aaccgaggta tttgccgaag atggaaaaat aaagcttcgc tcggagtacc taaacgatac
     2461 cctaaacgga gtgtggcaga cctactatga atcgggagag ctaaactata gcggaagcaa
     2521 aagtaaaggt aagtacgaca acgtgctgct ggcttaccat aaaaatggat cgcttcagcg
     2581 cgctgcacga taccacgatg gggttctgga cggcgaggta gtttcgtact atgaaaacgg
     2641 taagcctcag gtaacctcgc acttctcaat gggaaagcaa acaggcgagt ttgttgagta
     2701 ctaccccaac ggctatgtta gggaaagggg aatgtacaaa gagaaccaaa aggagggcat
     2761 gtggatgagc ttttacccta acgggcagct tcatggaaag ggaaatttta aacagggtag
     2821 ccttatcggc gaatggatat tttttcatga taacggaaac ctcaagcaac gtggaagcta
     2881 ctgcaatggt aaggcttgtg gcttatggca gtacttcaac cctcaaggaa atttaatttt
     2941 gtgcggagtt tacgtcgatg atttacaaaa tgacatttgg actacctttt acccaaatgg
     3001 ttcggtagct ggcagaatac tttttgtaaa caacaaacct gctggaataa gatgggttta
     3061 ccagcaaaat ggatcaatgg agaagagagg cggacagctt taggttcttc aactcaaccc
     3121 cacaataaca aataagaggc tgaatgtcag cctcttattt gttatataac gtttatactc
     3181 caacaggcat tttaggcatc aatgttggcg tacttggcat gtgcttcaat aaactcgcgc
     3241 ctaggcggaa cctcatcgcc catcagcatg gagaatatcc tgtcggcctc agctgcactt
     3301 tcgatggtaa cctggcggag gaatcgggta tcgggacaca tggtggtaat ccaaagctgt
     3361 tcggcattca tctcaccaag ccctttgtag cgctgaacgt gaacagagct atcacgtccc
     3421 ttcccgagct cctgaactgc ctttagcctc tcctcctcat tccagcagta gcgatcctcc
     3481 ttaccttttt taaccaggta aagcggggga gttgcaatgt agaggtgtcc ctcgttaata
     3541 agatcggtca tgtagcggaa gaagaaagtc atgataagtg ttgaaatgtg gcttccgtcc
     3601 acgtcggcat cggtcataat gattacttta ttgtaacgaa ggcccgacag gtttaaggcc
     3661 ttgctatcct cttctgtgcc aatacttaca ccgagtgcag taaaaatatt ccgaatttcc
     3721 tcgttctcaa atattttgtg ctgcatggcc ttttcgacgt tcaaaatttt tcccctcaag
     3781 ggcaggatgg cctggaagct cctgtcgcgt ccctgcttgg cagtgccgcc agccgaatca
     3841 ccctctacca gaaaaagttc tgttttcagg ggatccttat cggagcaatc ggctagctta
     3901 cctggaagtc ctgagccgga aagcactgtt tttctttgca ccagttccct tgccttgcgg
     3961 gcagcatgtc gtgcctgtgc ggcaagtatg accttttgaa caatgttgcg ggcgtcgcgg
     4021 gggttttctt ccaggtagtt tgccagcgct tcgcttactg cctgatcaac agccaggcta
     4081 acctcggggt tgccaagctt ggttttggtt tgaccctcaa actggggttc ggccacctta
     4141 acggatataa cagccgttaa tccctcccgg aagtcatcac cattgatgtc gaatttcagc
     4201 ttggaaagca tccctgaatc atcggcatac tttttcagcg tccgggtaag cgctcttcga
     4261 aaaccagtta ggtgtgtgcc cccctcaacg gtattgatat cgtttacgta ggagtggaca
     4321 ttttcggaaa acgaggtgtt gtactgcatg gccacctcaa ccggaacatc ccctttatcg
     4381 gactcgatgt aaatgatttt ctcggtcagg ctctcccggt taccatcgag gaacttgata
     4441 aactccttta acccctcaac cgagtaaaat tcctcgtggc ggtagtgggc ttcgccgttt
     4501 tcaaccaatt ctcgcttgtc agtgagtgtt aggcgaatgc ccttattgag gaatgccaac
     4561 tcgcgtaaac gcgaggcaat aatttcaaag ttgaagctaa tggattgggt gaatatctgc
     4621 gaatcgggca tgaaggtaat gatggtgcct gtgctgggtg actctccaac cacctcgagc
     4681 gaagtaacag gctttcccat tgaaaattcc tgacggtata gctttccatc gcgcataacc
     4741 tcggcaacaa gctttatgga gagggcgtta acacaagaaa ctccaacccc atgcagccct
     4801 cctgatactt tatagctgcc cttgtcaaac tttccgccag catgtagaac ggttagcacc
     4861 acctcaagtg ccgatttctt ctccttttca tggtagtcaa ccggaattcc cctcccatta
     4921 tccactacgg tgattgatcc atcctcgttg atggtaacat caatcctatt gcagaaaccg
     4981 gctagcgctt cgtcaatgga attatcaact acctcgtaaa caaggtggtg taatcccctg
     5041 atgctggtgt cgccaatgta cattgatggt ctcatgcgaa cggcctccag cccctcaaga
     5101 acctgtatgc tatcggcgct ataaccgttg gaactcaact cttctccctt caaaccttcg
     5161 ctgctcatac tacttataaa tatttggaat cagttgcaca ctttagtatt gtaattaata
     5221 agctaatttt taaaagccat caaaagtagt aaaaattttg gttaaacagc acttcaaaaa
     5281 ttactttcca ataatcatat agtgctatta tacagacaca tacgtatttg ttgataaaat
     5341 atttcaaaaa gtatgcccgg gttacagcag ctgtttgttg ttctgttttt aaaagaagaa
     5401 aagcttgaga gaaaaagcta acttcgcagc aaaacctgag gtagcccatt tggcatagtt
     5461 gaattttatt acctcaaata gaacttacac catgacccaa tatcttgaac tactagaaca
     5521 tatattgaag aacggagtgc agaagaccga caggacgggc acaggaacaa tcagtgtttt
     5581 tgggtaccag atgcgctttg atcttagcag agggtttcct ctgctcacca caaaaaaact
     5641 acacctgaag tcgatcattt acgagctgct gtggttttta agaggagata ccaatattaa
     5701 gtatctaaac caaaacggcg taaccatttg ggacgagtgg gccaaccgcg aaggggagtt
     5761 ggggccaata tacggctacc agtggcgctc gtggccagct tcagatggcc ggcatattga
     5821 ccagatagca caagttgtcg gctcaataac ccaaaatccc gattcgcgca ggcatttggt
     5881 ttgctcgtgg aatgtaggcg atataggtaa aatggcgctt cctccttgcc atgtgctatt
     5941 ccaattctac gttgctggcg gtaagctctc ctgccagctt taccagcgca gcgccgatgt
     6001 ttttcttgga gttcccttca acattgcttc gtatgctctc ctaaccacta tggtagcccg
     6061 tgttaccgga ttagaacccg gtgaatttat ccatacgctg ggtgatgccc acatctacct
     6121 caatcatgtt gaacaggtta agctgcagct gacgcgcgac ccgcgcccgt tacccaccat
     6181 gcacattaac ccgcaggtga aatctatttt cgatttcagc tatgatgact ttagcctgga
     6241 gaactacaac ccgcatcccc atatcaaggg gaatatcgcc gtttagtgat actcgactgt
     6301 gaatctcgaa atctctccta caagaattaa ttaaaatttc acatcatatt ttttggtaat
     6361 acattaaaaa gctatttttg tgatgaattc agtttaacta aaacttgcag aaatggcata
     6421 caaaatttcc gacgattgca ccgcttgtgg tacttgcatt gatgaatgtc ctgttgaggc
     6481 aatatccgag ggcgatatct ataagattaa ccccgatatt tgcaccgact gtggttcatg
     6541 tgccgatgtt tgcccggtag aggccattca tcccgagtaa ttacagcagt aattttatca
     6601 aaatccgcaa atcatgcgga ttttgtttta tatatgaata ccccctgatt ggtagcatga
     6661 cgaactgaac aaaccatgct cctttgagct aaaaccgctt caacaatgca aatcaaaaaa
     6721 agaaccgcac aaccttttcg aaaaccttta atgctggcag tacttgcaat gagcgccacg
     6781 ctaagctttg gtcaaccatt cggcgtgcta acctacaaca ttcgcctcga taccgagcag
     6841 gatggggtaa atcgatggga taataggaaa gaatcaatgg tcgagctagt caaccgttac
     6901 agccccgcca tatttggagt tcaggaagcg ctgtaccatc aggtctgcta ccttgatagt
     6961 gccttaaaaa aatacaccta cattggcgtt ggcagagacg atgggaagaa ggccggcgaa
     7021 ttcagcgcta ttttcttcga caacaccagg cttagggtga ttgcccactc caccttctgg
     7081 ctatcggagc accccgatag cgtttcatta ggctgggacg cagcctgcag aagagtttgc
     7141 acatacgggc tttttgagga catgctttcc cataaaaaat tttgggtttt caacacccac
     7201 ttcgaccatg tgggtaaggt tgcccgtaaa aattccgcag agttgatagt caacaggata
     7261 aatgagctga atggcgataa tttgccggta atagcaatgg gtgacttcaa cagcaatccg
     7321 gctgacgaac cggtgaagat attagcctca acgcttgccg atgggttaac ttgctctcaa
     7381 aagccgttga atggaccggt tggtaccttt aacgggttta accctgaata cctacctgat
     7441 gaaaggattg actatatatt tgttctgaaa gtgcgagtgc tcacctacag ccatcttacc
     7501 gaccgaaggg aaaatggtct tttaatctcc gaccatcttc ctgttcttgc tgaggtggaa
     7561 atatgaatac cttcaattta agattttatt gaatttacta gagttgtctt agcctaaatc
     7621 atggcattgt ttcggcctgt tatttttttt tggcaaaagt gattctagag tctgaaaaca
     7681 accccatcaa cgcagctctt taacctttgg ctaccagagt attctgaatg caggggtatg
     7741 ctccgtcgtt ctgcttcttt caagtcgagt tgaggcaggc attgctgcgg aaccgaccgg
     7801 aatagaccct ttaccgacag gtagtgcgcc aggtattcct gctcggtttg gccgggagtg
     7861 ggaataagca atcctgaaat gcctagggta acaagatcca ttatggtgct ataaccaccc
     7921 ctgaccagca ggtacctaca gctacgtaac acgaatgcca gctgctcgtc gggtaaatgg
     7981 ccggcatacc atattccgtc aaccactcta agttcactac cattttgagg tttcccctcc
     8041 accaccaggc aaccaatgga gtaacggtgg gccaggtttg ctgccagctc aaccagaagg
     8101 gcacgctggg gctcgggacc cgaaaccata actaccattt cccaggtttt aaaattggat
     8161 gctggcgcta tccctgaaaa acgtgatagt attccgatgt attgaatgtt ggtcaatccg
     8221 ttagggctgg acagcactcc ggcaatactg ccagcaccct cattatcggg aatccaaaca
     8281 tggttgaagc gtttcaacca atgtcttatt agtatttccg acgctttagt aaggaatttg
     8341 aaaggtctgg gcggaattac cctaagctgg tgggttacca tcaccgacat aattccacgc
     8401 agccacaacc cgtagcggtt gtcggatatc accaggtcaa tgtggttttc caaaaccagc
     8461 tttttcagct ttctatgttc ccttaggttg atgaagggaa gcctgatggc aacccaaagc
     8521 ataggcagaa gctggctgga gccccgctta aattgtacct tgtacgacgg aaaaaatttg
     8581 gtttctaagt aagggcatga gctttttacc agagccagcg aattcgcatc ggctgcaacc
     8641 accacccgat gtccgctttc cagcagtaag cgaacaatag ggacaaccct gcttgcatgg
     8701 ccgagccccc atccaagagg gcatactaaa atgttcagct gccgaggggt gctcattatt
     8761 taggctgaaa cctgtgttgc aatacccaac atctccacct tttgggcaat gccgttgagt
     8821 tcctcctcag atagcttttt gaggtcattg gccggggtat cgcgggcaat ggtgtaaatc
     8881 atcacctcgc taggtcgaat ctgatcaacc agcttgagcc atgccgagat ttcggcaggt
     8941 gtggtgttgt caaccggtat tccgttgatg ctgcccctta cgaataacga ctggattatc
     9001 aatttccctt caaaagccat caagcggtca acaagatttt ttaaatcaaa acggatgagg
     9061 ggttggttga gagcgttgat ggttgcctcg aaggcagaat cgagttttaa aatgttctta
     9121 tccactcttt taagggctgc aaccacatcg ggacgatgca gcatggtggc attagagagt
     9181 acagcaatgt cggcattagg gaagtatgta ttcctcaact caatagtatc gtctataatt
     9241 ccggaaaaat caggatgaag tgtaggctca ccattgccag caaaggtaat tacatcaagg
     9301 ccttcacccc cgctaatcat tttctttagc ttatactcaa gggctgcctg aacctgcccc
     9361 cgtgaaggga gcatatccga atcaatggag tcggtccagc cacactcgca gtacacgcag
     9421 ttgaacgagc aaacttttcg gtcgggcgga agcaggttca ttcccagcga ggcaccaagc
     9481 ctccggctac gcaccgggcc aaacacaatg ctatcgaaca gaaaggttga catagctgct
     9541 gatttttcgc aaagataccc ttttggagca agatggtgtg cccgtgttca acttttggaa
     9601 attatgaaca attcaaagct tgacattcta ttaaatacca atgtgttggg tatgctgcat
     9661 acacgatact aaagcacgaa ccagctgatt catcaacttc tgcataacaa tctataaaaa
     9721 ttatcggcgg caacccccaa ctttaaatcg tttggggttg ccgccagatg aataaaacct
     9781 ccagtgggat taactcctaa aggtggaaat ccccttaatg cttattaaaa cttgtaaata
     9841 acgcctagcg atatgctaga catgtcgaaa ttcaagccaa aaccgttagt ttttaaagtg
     9901 tgagtttctc cggcaatctc atccttttcc ctgttaaaag aatagtacac actacccagg
     9961 aaggcttcaa tagccagctt ttcgcccaag ctgtaggaga tggcaggcat aacgctaata
    10021 ccaaaattta agtatttagg cccatcagta gtattcccat cattcttcgt tttgttggcg
    10081 ccaaactcta ttccgacatt accttgtgca aaaatattca catcacccag gcttacagcg
    10141 tagtacctga caaacggagt aattccaaac atgctggtaa gattggccac atcgttttgc
    10201 ttttgctttt gaaggctata gctaacacct gcacctactg ccaacttgtc gtttaaaaaa
    10261 tacccgactg caggggcaac acgaaaatag gtgtatcgtg ctccctcggt tgtaaccgac
    10321 ccggttgtag tttttggcat tgaggttgaa aggttgatgc taccgctaac aaacatttgc
    10381 gcatttacta cgcctgcgat cgtggttaat gcaattaata caaaaaaact cctgatgatt
    10441 ttcattacga ttcatattta atgttaggcc tactcttagg tttttcggca tacacctata
    10501 attaaactca gcaattgacg tattgttcaa tttcgatatt atgacggtta tagggtttaa
    10561 gccatcccta ccatgttatc gccgatagct acttttgcct agaaaaatta tttcaactta
    10621 agtaaatttg caacagcaaa aattgcgcca actgccatta ggctagaggc tgaatccccc
    10681 tttacgtatc aagtttgctc tgctgattcc tatgccccag agtgttatgc gaattgcttc
    10741 ccacaagtag tatgcatttg gtaagccatt agctggtttg atggaaagaa aatacacggt
    10801 ttaagaataa gtttttatat catgcaaaac gttaaacaca aatggatttg acctgtttag
    10861 taagcaaaaa atggctgtgg aaagaaaaat atacccggta gaaggaatgt cgtgcgccag
    10921 ctgcgccatg accgtggaaa aaaccataaa gaaccaaccg ggggtggagg atgcgggcgt
    10981 taactttgcc aacagcacgg tttgggttac ttttgatcct aaggctgtta aacctgaaga
    11041 tttaagggat tctgttcgtt cggctggcta cgatctcata gttgatgccg ttgacgtggt
    11101 aaaagttggc gaggaagcca agcagaaata cgccgatgag ctaaaactgc gcttctggat
    11161 gtcagcagca ttctcagtgc ctcttctcgt gttgggaatg ttctttatgg attggaagct
    11221 aaccccgtgg ctgtcggcca ttatggctgc tccggtggta ttttggtttg gcaggcaatt
    11281 ctttactaat gggtggaagc aggcccggca tttcagggca aacatggata ccctggttgc
    11341 gctaagcact tcaattgcct acctctttag tctttttaac actatttacc cggagtattg
    11401 gacaagtcga ggccttcagg ggcatatcta ctatgaatcg gcttctataa tagtagcctt
    11461 tatactgcta ggaaagtggc ttgaagagaa agctaagaat caaaccacct catcaataaa
    11521 aaaaatcatg ggcttgcaac ccgatatggc ctggttggtt gagggagaga gcctgagcga
    11581 ggtaccgcta tcccgtatta ggaagggcga tgtagtgctg gtgaagcctg gtagtagagt
    11641 ccctgttgat ggaagagttg tggatggcca aagcgtggtt gatgagagca ccatttcggg
    11701 cgagtcaata cctgttgtca aaaaagtggg tgataaagca tgggccggaa caatgaacca
    11761 acggggcagc tttaagttga tagccgaaca ggttgggtcg gaatcggtgc tgggtagaat
    11821 catcgctaca gttgaaagcg cacagaacag caaagctccg gtgcagcatc tggttgacaa
    11881 ggtggcagga atatttgttc cagcggtaat gctggttgcc ctacttaccc ttattacctg
    11941 gcttgtgcta gatccggcta acggcttctc acatgggcta atctccatgg ttaccgtgtt
    12001 ggccatagct tgtccgtgcg ccttgggtct ggcaacgcca acagccatta ttgttggagt
    12061 tggacgggca gcagaaaaaa acatcctgat tcgggatgcc gaaagcctcg aaacagccca
    12121 ccaggtaaac tacattgttc tcgataaaac agggacgctg agcgaaggcg tgcccagcgt
    12181 ggatagaatc gaatggttta cccatgaaac cacctatccg ggtattttaa aatttattga
    12241 gcagaattcg ggtcatccac tggccgatgc tattgttcgg catttcgaaa cagacgactc
    12301 ctcgggattt gtggccgata gctacgagtc cattgccggt aagggggtaa agacaacagt
    12361 aaagggaaaa aagtatttgg ttggcaatcc agactttctt aggcaggaag gagtgatgtt
    12421 taccgataca attgaaagca gcctttcgga gattttaaac aacgccggat cggttgtagg
    12481 gtttgccgaa aataaccttc ttctgtgcct gattcgggta tcggacagga taaagccctc
    12541 ctccagggaa gccataaaaa gattaaaggc cagaaacata gaggttgcca tgctcacagg
    12601 cgacaaccgt gcaaatgcgg aatttatggc acgggaactc gagattgccc actatgcagg
    12661 gcagcagctt ccgtcggata aggctgattt catcaaaacc cttaagaaac agggaaaagt
    12721 ggttgccatg gtgggtgacg gcgttaacga tagcgaggcg cttagtgtag ctgacgtagg
    12781 aatagccatg ggcaaaggct ctgacattgc cattgacgtg gctcaggtta ctatccttaa
    12841 ctccgattta aacatgctgg taacgctgct cgatatttca ggcaaaacta tcaggacaat
    12901 ccgccagaac cttttctggg catttatata caacatcatt ggtattccaa ttgcagcggg
    12961 acttctttac ccattctacg gaataaccct agacccaatg tttgcaggta tggccatggc
    13021 tctcagctcg gtgtcggttg taacgaacag ccttcggctg agaaccgcca ggatttgaaa
    13081 aaggacaaag tgagtttgta cggctaatga ctgattcaat ataaaaagga taaaggttaa
    13141 aaaacttgtc cgcctaccgg cggaacttgt ccggctcctg acggattcaa gaacttgtcc
    13201 gcctaccggc ggaactt
//
LOCUS       DAOPHX010000068        12964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_5665_length_12964_cov_5.599814, whole genome shotgun sequence.
ACCESSION   DAOPHX010000068 DAOPHX010000000
VERSION     DAOPHX010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 12964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12964
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5665_length_12964_cov_5.599814"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PK502_06385"
     CDS             complement(<1..336)
                     /locus_tag="PK502_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPH00478.1"
                     /translation="MEASEILKRVRKIEIKTRGLSQHIFAGQYHSAFKGKGMAFSEVR
                     EYRYGDDIRSIDWNVTARYNQPYVKVFEEERELTVMLLVDVSGSQQFGTDFLLKRQAL
                     TEVAAVIAFS"
     gene            complement(342..761)
                     /locus_tag="PK502_06390"
     CDS             complement(342..761)
                     /locus_tag="PK502_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00479.1"
                     /translation="MSIFRILPLALLLVNLSIPMQGQNLIGLTKGEILKLVPTSCKGF
                     NFVKDIQNGDKSFIKFENPTDEQTLLFMLNSKEICTSVSRMYNTWLYDKVYKDLCCEY
                     KQVSMDTWIDSKNGNDYEVKLKRGEWFITVTIRPLIK"
     gene            complement(763..1764)
                     /locus_tag="PK502_06395"
     CDS             complement(763..1764)
                     /locus_tag="PK502_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPH00480.1"
                     /translation="MSDTAAVNIKELNERIRLESSFVDIINIEMGKVIVGQKHLVESL
                     LVGLLADGHILLEGVPGLAKTLAITTLSNIIDVKFNRIQFTPDLLPADLIGTLIYSPR
                     SEQFNVKKGPIFANFILADEINRAPAKVQSALLEAMQERQVTIGDQTYNLEEPFLVLA
                     TQNPIEQEGTYPLPEAQVDRFMLKVKISYPKQEEERMIMRENLLQTFPKPERVLKPAD
                     IINARKVVREVYMDEKIEKYILDIVFATRFPADVNLQKFAPMISYGASPRATISLAIA
                     SKAYAFIKRRGYVIPEDVRAVCHDVLRHRIGLTYEAEAESITSEDIITEILNSVEVP"
     gene            2086..2853
                     /gene="truA"
                     /locus_tag="PK502_06400"
     CDS             2086..2853
                     /gene="truA"
                     /locus_tag="PK502_06400"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPH00481.1"
                     /translation="MEQSTIYRYFVELKYRGTRYHGWQVQANANSVQGEVNRALSTLL
                     EAPTETFGAGRTDTGVHARFYVAHFDSTAAGLDADPTLLYHLNRILPADIAITRIIRV
                     RQDANARFDALYRGYEYLICREKSPFLNGLAWMYYGHLNVEWMQLATQQLVGYSDFTS
                     FSKLGSNSKNNTCKIYHASWHQQNDMLIFSIKANRFLRNMVRAIVGTLVDVGRGKITP
                     EEFASIVEARDRSLAGTSAPAEGLYLTEVKYPKEMFL"
     gene            complement(2953..4491)
                     /locus_tag="PK502_06405"
     CDS             complement(2953..4491)
                     /locus_tag="PK502_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HPH00482.1"
                     /translation="MLTKLDLKQLEERGITPQQVEGYMRMFREGFPFLPIERPATVGD
                     GIISLEDDEVDDLVEMFKDFQGSKVKFVPASGAATRMFKHLFEFRDEYPVKGLECFND
                     KGFNSAYSFFQNIKRFPFYDKLYNELWSVGYRLESLLEKKDYLPVVNALLDSNGLNYG
                     SLPKALIHFHRYGEIARTALEEHLVEAAKYARDQHGVANLHFTVSPEHLKGFQELVER
                     VKSFYENHYEVTLNVAYSVQKPTTDTLAVDMENNPFRNPDGSLHFRPGGHGALIENLN
                     ELNEDIIFIKNIDNVTPDHLKAATIRYKKALAGLLVSYKNLVNGYIERLLNEPVSEEQ
                     LALMLEFTTDELCILPPANLNRQNSDAVKKYLMEILDRPIRVCGMVKNQGEPGGGPFW
                     AKNPDGTTSLQIVEQSQVDMGNPRVKEIFGRSTHFNPVDIVCSIRNYKGEKFNLSNFL
                     DPQAGFISIKSKNGKQLKALELPGLWNGAMSRWITFFVEVPIATFTPVKTVNDLLRSE
                     HQPE"
     gene            complement(4544..5311)
                     /locus_tag="PK502_06410"
     CDS             complement(4544..5311)
                     /locus_tag="PK502_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPH00483.1"
                     /translation="MDLNSYSLPQYLTRSMVVQSYEVDFNQRFSITSIFNHLQEIAWE
                     HADKLSYGYNHLREKGWFWALSRVEVEFYRFPVWQEAVTLVTWPRGADGIFALRDFEF
                     YDSNGNKLIAATSSWLVVGVENRRPVRIAEWYLESDFASRSALNRNAAKIPESIVQPA
                     FTEQFEVRAGDIDMNLHVNNVRYIDWAYNTFTISSLRKYFPKKISVNFNSEAKEGDSI
                     LINRKNIPGNSTLININSRLEGEKNYCRIQFDWRNLE"
     gene            complement(5359..5748)
                     /locus_tag="PK502_06415"
     CDS             complement(5359..5748)
                     /locus_tag="PK502_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPH00484.1"
                     /translation="MEFTIPIGAECIVPEVVSSDNIASKYGSGMVEVFATPAMVALME
                     KAAMIAVRPFLPEGYSTVGVEVCVKHTKATPIGMEVWSKAVLKEMDGKRLTFEVKAWD
                     ANGEIGTGTHIRYIIDSRRFMEKIAAR"
     gene            complement(5814..7004)
                     /locus_tag="PK502_06420"
     CDS             complement(5814..7004)
                     /locus_tag="PK502_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPH00485.1"
                     /translation="MHYSVPVSNKIHWIGVNDRRKRLFENLWPLERGVSYNSFLIVDE
                     KTALVDTIDGTAVGNYIERIEQLLNGRPLDYLIVNHMEPDHSGEVKAIIDRFKGVKLV
                     GNVKTFKIVEAYWGITEGLIDVEDGRTLDLGYHKLKFLMTPWVHWPETMMTYDTTDQI
                     LFTGDAFGSFGAFEGGIFDDELDPLYFEDEARRYYSNIVGKYSNMVQKAIAKLEGIPV
                     RTIAPTHGPVWRSNPTIIMDWYNRWSRFEAAKGAVIIFASMYGNTEDIADYLARRLCE
                     NGVRNIRVFDVSKTHISYLISEIWKFNGLVLGSCAYNSQMFPLMEQLTHELLHIGLKS
                     KHLALFGSYSWNGGGVKNLAKFAGESELNLVSEPLEIYGKPTPQKLKPFDTLAETLAH
                     KILE"
     gene            complement(7257..8636)
                     /locus_tag="PK502_06425"
     CDS             complement(7257..8636)
                     /locus_tag="PK502_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPH00486.1"
                     /translation="MRNSKNFTGHQLLMAILLAFILVFSSCKYSAKEEIPFQVQHADW
                     VYNATIYEVNVRQYTPEGTFKAFESHMPRLKELGVEILWFMPIHPIGEVNRKGALGSY
                     YSVKDYYGVNPEFGTLDDFKSVVAQAHQLGMKVIIDMVANHTSHDAVLVKEHPDWYVH
                     DSLGNLVSPFDWTDVAKLDYTKPELRKYMIDMFKYWIKDIDIDGFRCDVAAEVPVDFW
                     NEARAELNRIKPVFMLAEASNVELQKFAFDADYDWELHHTLNALAQGKKNVNDLEQYL
                     LKKYATYPRNTIKMNFITNHDENSWNGTEFERMGDAVKAMAAFTFVIDGMPLIYTGQE
                     VGFNRRLKFFDKDEVSWKDSLNLFPFYKKLVELKKSTPVLAAGDRGADIFRIKSNADS
                     AVFAFTRKNESQALFAIFNLSANQQIATLTGNDYAGTYTNVMTGEKFVFQGGEEISLK
                     PWEFYIYCN"
     gene            8932..10026
                     /locus_tag="PK502_06430"
     CDS             8932..10026
                     /locus_tag="PK502_06430"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPH00487.1"
                     /translation="MNRVLVATGGGDCPGLNAVIRAVVKRASQEPDWEVIGSIQSFDG
                     ILREPTEIRVLDEKAVSGIHVQGGTIIGTTNKGGPFAWPIKNKDGSWGAVDRSDEMIR
                     KLQYLGIDAVISIGGDGSQKISQQLYEKGLNIIGVPKTIDNDLSATDFTFGFQTAVQI
                     ATDAVDKLVTTASSHNRVLILEVMGRFAGWIALHAAIAGGAEVCLIPEIPYDINKVAE
                     KLNSRYNKGKGHAIVVIAEGAKPIGGTLTSEQSDEVGYNNLRLGGVAHSLTNDLKRIG
                     FEADIRETVLGHLQRGGIPVAYDRVLATQFGVKAFEMVLQGKFGEMVAYRHPDIISVP
                     LIDAINRPNYVDPNCDLVRTARGVGISFGD"
     gene            complement(10110..10517)
                     /locus_tag="PK502_06435"
     CDS             complement(10110..10517)
                     /locus_tag="PK502_06435"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPH00488.1"
                     /translation="MEKLYIEPKKDSPEVIFDPKNGNFSITGISHPENVATFFEPIFS
                     WLEAYFVELGRTDSIRMKPINVRLFFKYINSATFKQLVTLMQKFKQFVETGYKVNITW
                     QFEPDDEDMKEAGYELIEISGINTTFSCEVCNN"
     gene            complement(10535..10918)
                     /locus_tag="PK502_06440"
     CDS             complement(10535..10918)
                     /locus_tag="PK502_06440"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPH00489.1"
                     /translation="MVERFFNEPSAVTPLVEFDQQQGIFKIQGKSLPEDVKAFYMPLL
                     QWWDEYRNSPNDTTNLVLDFDYFNTASSKMILLLLSKLKDLSLLGKNVRVTWCFPSHD
                     SEMEEAGEELAEILNIPFDFIPKNS"
     gene            complement(10934..11323)
                     /locus_tag="PK502_06445"
     CDS             complement(10934..11323)
                     /locus_tag="PK502_06445"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPH00490.1"
                     /translation="MDKFYIKGTAETPTVSFDSTGNTFEISGKSLPEDVKEFYHPIIK
                     WLTEYCQSPNDATTLKVRMEYFNTASSKMILELFELLNKLHLSGRNAAIEWYYQEDDE
                     DMMDAGNDYADMLDLPFKIFGYQTLLK"
     gene            complement(11473..12810)
                     /locus_tag="PK502_06450"
     CDS             complement(11473..12810)
                     /locus_tag="PK502_06450"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HPH00491.1"
                     /translation="MQYNGFIFDYARCVGCHACVVACYNENGVQPPVLWRQVVGVNPE
                     KVPLYGFVSLSMACNHCTDAPCLKACPASAYYRDQETGAILHSNKRCIGCKYCTWACP
                     FDAPKYNPLRGVVEKCTMCNERLKSGEIPACTEACPTGALSFGSIETAASIQAPGISA
                     KPFSPRIVTKNEDIVNHIPHIDEQVTGYSPLQHTGMKHFAPSKINPIHELPLAIFTLI
                     CSLMVGWVGSMPWVGIASRGITLFILLGIVGALLSTFHLGKPFRALRSVANVKSSWLS
                     REILLFTLFFSLSALNLIFFNLIVLSVIAVILGLLLLVAVEMVYSVTDKKYKIHVHSA
                     NVVITALTIFSIFNSFERLLVLVLVLKAALYILRHANYSVSSLEFIFSFIRVFLGILI
                     PLGSIFFSNQHISMLMILFILIGELIDRIEFYGEQQVDTPRLLANNFWDKSSV"
BASE COUNT         3814 a         2799 c         2694 g         3657 t
ORIGIN      
        1 agaaaaagca ataacggcag ctacttcagt caaagcttga cgtttaagta aaaaatctgt
       61 tccaaattgc tgcgagccgc taacatcaac cagcaacata acagtaagct cccgctcctc
      121 ttcaaaaact tttacatagg gttgattata gcgcgccgta acgttccagt caatgcttcg
      181 aatatcatcc ccataccggt attccctaac ttcgctgaaa gccataccct tacccttaaa
      241 tgcgctatga tattgacctg caaagatatg ctgggataga ccgcgagtct taatctcaat
      301 ttttcgaacc cgtttcagta tttcagatgc ttccatacag cctattttat caacggtcga
      361 atagtaactg taataaacca ctcaccccgc ttgagcttca cctcgtaatc gtttccgttt
      421 ttactgtcaa tccaggtatc catgcttacc tgtttatact cacaacacaa atctttataa
      481 accttgtcgt agagccatgt attgtacatt cttgagaccg atgtgcatat ttccttgctg
      541 ttgagcatga aaaggagagt ttgctcatcg gtgggattct cgaactttat aaaactttta
      601 tctccattct ggatatcctt aacaaaatta aatcccttac agctggtagg tacaagcttc
      661 agaatttcac ccttggttaa gcctataagg ttttgcccct gcatgggaat gctgagattc
      721 acaagcagta gagccagagg caaaattctg aatatgctca ttctagggta cttcaaccga
      781 attaagaatt tcggtaataa tatcctcaga agtgatgctt tcggcctccg cttcgtaggt
      841 aagcccaatt ctatggcgca gaacatcgtg acacacagct cttacgtctt cgggaataac
      901 gtaaccccga cgtttaataa aagcatatgc cttagaagct atagccaggc taatagttgc
      961 acgtggtgag gctccgtagc taatcatagg agcaaacttc tgcaggttaa catcggcagg
     1021 gaatcgagtg gcgaaaacaa tgtcgagaat gtacttctca attttttcgt ccatgtaaac
     1081 ctcgcgcaca acctttctgg cattgataat atcagcaggt tttaaaactc tttcgggttt
     1141 aggaaaagtt tgaagcaggt tttccctcat aatcattcgc tcctcctcct gtttagggta
     1201 ggatatttta accttaagca taaagcgatc aacctgagcc tcgggcaagg gataagttcc
     1261 ttcctgttca ataggatttt gggtagccaa aaccaggaaa ggttcttcaa ggttataggt
     1321 ttggtcgcct atagtaacct ggcgttcctg cattgcctca agtagagcac tttgaacttt
     1381 ggccggggca cgattaattt catcggctaa gatgaagttg gcgaaaatag gtcctttttt
     1441 tacgttgaac tgttcgctcc ttgggctgta tattaaggta ccaataaggt cggccggaag
     1501 taaatcgggg gtaaactgaa tgcggttaaa cttaacgtcg ataatgtttg acagggttgt
     1561 aatggctaat gtttttgcta atcccggaac accctccaac aggatatgtc catcggctaa
     1621 aaggccaacc agcaaagatt ctacaaggtg tttctggcca actattacct tgcccatttc
     1681 tatattaata atatctacaa atgaactttc cagacggatt cgctcattta gctccttaat
     1741 gttaactgca gctgtatcgc tcatataagt cagtttaatt agtattgtag tggttatgaa
     1801 ttaaaacgaa tgtttgcaaa tatgataata aatgaaggcc aatggtgtta aaaaaagtta
     1861 aatcaaaatg agtgatataa taaaggaagt taatgggttg ccggttgaaa ataagaattt
     1921 taaaatctag catgagctgt tacaggtagt aatgtccaga accaccggtt cgcatctgaa
     1981 tgtccatgca tagccttaaa caagaattgc aaaagccgtt taacccgaat tgtcaagttg
     2041 tgctatattt aaccttgtaa ttgataatgc taaattcgca ttgaaatgga acaatcaact
     2101 atttacaggt atttcgtcga gcttaagtac aggggaacac gctatcatgg ctggcaggta
     2161 caggcaaatg ccaacagtgt tcagggtgaa gttaaccggg ctctttcgac attgctggaa
     2221 gccccaaccg aaacctttgg cgccggccgt accgataccg gtgtgcatgc ccgtttctac
     2281 gtggctcatt ttgattcaac agcagctgga cttgatgctg acccaactct gctttaccat
     2341 ctgaatagaa ttcttcctgc tgatatagcc atcacacgaa taatcagggt aagacaggat
     2401 gcgaatgcac gctttgatgc attatatcgg gggtatgagt acctgatttg tagggaaaaa
     2461 agccctttct taaatgggct tgcgtggatg tactatgggc atttaaacgt ggaatggatg
     2521 cagctggcaa cgcagcagct agttgggtat tccgatttta ccagcttcag taagctgggt
     2581 tctaatagta aaaacaacac ctgcaaaatt tatcatgcca gctggcatca acaaaacgac
     2641 atgctgattt tcagtattaa agccaacagg tttttacgga acatggttcg ggcaattgtt
     2701 ggaacgctgg tagatgttgg gcggggaaaa atcacccctg aagaatttgc atcaattgtt
     2761 gaagcgcggg accgcagctt agcaggaacg tctgcacctg ctgaggggct atacctcaca
     2821 gaagttaagt accccaaaga aatgtttttg tgagcttacg gagaagaatt ctgtaaaaac
     2881 gattaatatt agccaaagca ttccttcaaa taaaaacttc agtcaaaatg atttaggagc
     2941 aactttccca agttattcag gctgatgctc acttcgaagt aaatcgttaa ccgttttaac
     3001 cggggtaaag gtagcaattg gcacctcaac aaagaaggtt atccatcggc tcatggctcc
     3061 attccatagg cctggcagct caagcgcttt aagctgcttg ccgtttttac ttttaatcga
     3121 aataaagcca gcttgaggat caagaaaatt agacaggtta aacttttcgc ctttataatt
     3181 ccttatgctg caaacaatat caaccgggtt gaagtgggtt gacctgccaa aaatctcctt
     3241 tacacgggga ttgcccatgt caacctgcga ctgttcaaca atctgcagcg aggtggttcc
     3301 atcaggattt tttgcccaga acggtccacc gccaggctcg ccttggtttt tcaccatacc
     3361 gcaaacccgg attggccggt caagaatttc cataaggtat tttttaacag catcgctatt
     3421 ttgcctattg agattagcag ggggcaaaat gcaaagctca tcggttgtga actcaagcat
     3481 tagggcgagc tgctcctcgg aaaccggttc gttgagcaat cgttcgatat aaccattcac
     3541 caaattctta tagctgacaa gtaatccagc aagcgccttt ttgtagcgta ttgtagctgc
     3601 tttaagatgg tcgggggtta cattatctat atttttaatg aagatgatat cctcgttcag
     3661 ctcattcaaa ttttcgatga gcgcaccatg cccaccgggg cggaagtgaa gtgaaccatc
     3721 gggattgcga aaggggttat tctccatgtc aacagcaaga gtatcggtag tcggtttttg
     3781 aacggagtag gccacgttaa gggtaacctc gtagtggttt tcgtagaatg acttgacacg
     3841 ttcaaccagc tcctgaaacc cttttagatg ctctggcgaa acggtaaaat gaaggttggc
     3901 tactccatgc tggtctcggg cgtacttggc tgcctcaacc aggtgctcct caagcgctgt
     3961 tcgggcaatt tcgccatagc ggtgaaaatg aatcagcgct tttggtaaag aaccatagtt
     4021 taaaccgttg gaatccaata aggcgttaac tacaggcagg taatcctttt tttcaagtaa
     4081 gctttccagc cggtaaccta cgctccaaag ctcgttataa agcttgtcgt aaaagggaaa
     4141 tcgtttgatg ttttgaaaga agctgtatgc ggagttaaaa cccttatcgt taaagcattc
     4201 tagaccttta acaggatact catcgcgaaa ttcaaaaaga tgtttgaaca tacgggttgc
     4261 cgctcccgag gctggaacaa acttcacctt gctgccctgg aaatccttga acatctcaac
     4321 caaatcgtcc acctcatcat cttcaaggct aataatacca tcgccaacgg ttgcaggcct
     4381 ctcgataggc aaaaagggaa atccctctcg aaacatcctc atgtaccctt ctacctgttg
     4441 aggagtaatc cccctttcct ccagctgctt caaatccaac ttggtgagca taatggttcg
     4501 gtttaccgtt aataaacagc gtcaatttac taacaattca cattcactcc aaatttctcc
     4561 agtcaaattg aattctacag taattttttt caccttccag cctactgttg atatttatca
     4621 aggtggaatt gcctggtata ttttttcgat ttatcaagat ggaatcgccc tcctttgcct
     4681 ctgaattaaa gttaaccgaa attttctttg gaaaatattt tcttagagaa ctgatggtga
     4741 atgtgttata tgcccaatca atgtagcgaa cattgttaac atgcaggttc atgtctatat
     4801 cacctgctct gacttcaaac tgctcggtaa aggcaggctg aacgatagat tcgggaattt
     4861 tggcagcatt acgatttaaa gccgagcggg aggcaaaatc agattcaaga taccattcgg
     4921 caattctcac agggcgacgg ttttcaacac caaccaccaa ccagctgcta gtagcagcga
     4981 ttagcttatt accattcgaa tcatagaact caaaatcacg aagtgcaaaa attccatcgg
     5041 caccacgagg ccaggtaacc agcgtaaccg cctcctgcca tacggggaag cggtaaaact
     5101 ctacctccac tcttgaaagc gcccagaacc aacccttttc tcttaaatga ttataaccat
     5161 agctgagctt atcggcatgc tcccaggcta tctcctgcag atggttaaaa attgaagtaa
     5221 tggagaaacg ctggttaaaa tcaacctcat acgattggac caccatactt cgagtgaggt
     5281 actgtggcaa gctatagctg tttaaatcca tactttaagg agagttgaga tagtttattc
     5341 tacaaaatat tcgcgaggct atctggcggc aattttttcc ataaaccttc tagaatctat
     5401 aatatagcgg atatgagttc cggttccaat ttcgccgttg gcatcccacg ccttcacctc
     5461 aaaggtaagg cgctttccat ccatttcttt caataccgct ttgctccaaa cctccattcc
     5521 aataggagta gcctttgtgt gttttacgca tacctccacc ccaactgtac tataaccctc
     5581 gggtagaaaa ggtcggacgg ctatcatggc tgctttttcc attaaggcta ccatggcagg
     5641 ggtagcaaaa acttcaacca tgcctgagcc gtattttgag gcaatattgt cagaactaac
     5701 cacctctggt acaatgcatt cagctccaat tggaatagta aactccatta tcaggtagtt
     5761 ttaaggttat atgaagtaga agctcatgtt ttaaaaggct gcgaaaaggc atgttactcc
     5821 aaaattttgt gagcaagcgt ttctgccaat gtgtcaaaag gctttaactt ttgaggtgta
     5881 ggttttccgt atatttctag cggttctgac accaagttaa gttctgattc cccggcaaat
     5941 ttagccaggt tcttaacccc acctccattc cagctgtagc tgccaaatag ggccaaatgt
     6001 ttgcttttca gtccaatgtg aagcagttcg tgtgtaagct gttccatgag tggaaacatt
     6061 tgagagttgt aggcacacga tcctaaaaca agaccgttaa acttccatat ctcgctaatc
     6121 aagtagctaa tgtgtgtttt ggagacatcg aagaccctaa tatttctaac accattctca
     6181 caaagcctgc gggccaggta atcggcaata tcctcagtgt tgccatacat tgaggcaaat
     6241 atgataacgg ctcctttagc agcttcaaag cgactccacc tattatacca gtccataata
     6301 atggttgggt ttgaacgcca gacaggccca tgggttggtg caatggttct aacagggatt
     6361 ccttcaagtt tggcaatggc tttttgaacc atattgctgt acttcccaac aatattggag
     6421 tagtagcggc gagcttcatc ctcaaagtaa agagggtcaa gctcatcgtc aaaaattcca
     6481 ccttcaaaag cgccaaaact accaaaggca tcacccgtaa aaagtatttg atcggttgta
     6541 tcgtaggtca tcatggtttc gggccaatgt acccaaggtg tcatcaggaa tttcagctta
     6601 tggtatccca aatcaagagt tctaccatcc tcaacatcaa tcaaaccttc tgtaattccc
     6661 cagtatgctt caacaatttt aaaggtttta acgttcccta ccagcttaac gcccttaaag
     6721 cgatctataa tggctttcac ctcgcccgaa tggtcgggtt ccatgtggtt tacaattaaa
     6781 tagtcgagag ggcgtccgtt tagcagctgc tcaatgcgtt cgatatagtt gcctacggct
     6841 gtaccgtcaa ttgtatcaac tagcgctgtt ttttcgtcaa ctattaaaaa tgagttgtag
     6901 gatacacccc tttcgagagg ccataaattc tcaaacaacc tctttcggcg gtcgttaaca
     6961 ccaatccaat ggattttatt gcttacagga actgaatagt gcataagaaa tagagtttaa
     7021 tatcattctg aagcacaaaa atagcaattt tagctggagg ctctattgat ttgaggacaa
     7081 agttatttta tgttgtaagg gaaagaagat tttagcacgg tgtgggttta tgataaatgg
     7141 gtcattgcag tatatgcacc aaactctaca atatattacg ttcatgttga tgcggcaatt
     7201 tacctaaaga aaaagggcgt tactactaac gccctgaatc acaaatattt gaaattttaa
     7261 ttacaataga tataaaattc ccatggtttt aatgaaatct cctccccgcc ttggaaaaca
     7321 aatttttcac ctgtcattac gttggtgtag gtaccagcgt agtcgttgcc agtaagagtt
     7381 gcgatttgct gattagccga gaggttaaaa atggcaaata gagcttgact ttcattcttc
     7441 cgtgtaaaag cgaatacagc gctgtcggcg ttgcttttga ttcggaatat atcagcaccc
     7501 ctgtccccgg cggctagaac aggcgtagat tttttaagct caaccaactt tttataaaaa
     7561 gggaaaaggt tgagcgagtc cttccagcta acctcatcct tgtcgaaaaa tttgagcctg
     7621 cggttaaatc caacttcctg cccggtgtaa attaatggca tgccatcaat aacaaaagta
     7681 aaggctgcca ttgctttaac ggcatcgccc atcctttcaa attcagttcc attccatgaa
     7741 ttttcatcat ggtttgtaat aaaattcatt ttgatggtgt ttctgggata tgtagcatac
     7801 ttttttaata agtactgctc taaatcgtta acattctttt tgccttgagc taacgcattc
     7861 agggtatggt gcagctccca gtcgtaatcg gcatcaaagg caaatttttg aagctccaca
     7921 tttgaggctt cggccagcat aaatacaggt ttaatccggt tgagctctgc tcgagcttca
     7981 ttccaaaagt caaccgggac ctcagctgct acatcacagc gaaagccgtc aatatcaata
     8041 tccttaatcc agtacttgaa catatcaatc atgtactttc gaagttccgg cttggtgtag
     8101 tcaagcttag caacatctgt ccagtcgaat ggtgaaacaa ggttccccaa gctgtcgtga
     8161 acgtaccaat cgggatgctc cttcaccagc acggcatcgt gggaggtgtg gttggctacc
     8221 atgtcaatta ttaccttcat tcccagctgg tgagcttggg caacaaccga cttaaagtcg
     8281 tcgagggtac caaactcagg gttaactcca tagtagtcct taacggaata gtagctacca
     8341 agcgctcctt tacggtttac ctcgccaatg ggatgaatag gcataaacca tagaatctca
     8401 accccaagtt ctttcagcct gggcatgtgg ctttcaaagg ccttaaaagt accctcggga
     8461 gtgtactgcc taacattaac ctcatagatg gtggcattgt aaacccaatc ggcatgttgt
     8521 acctgaaagg ggatttcttc cttggcgctg tacttgcacg aagagaaaac cagaataaaa
     8581 gcaagcaaaa tagccattag tagctgatgg cctgtaaaat ttttagaatt tctcatagat
     8641 gtaaatttta gtagggttac ttataactta tcattatatt cccaaaaaaa tgaaaaaatg
     8701 aaaaggaaaa ccatcaaatt tcaaacgatt gcaacatggc gtacttaata aatacagtag
     8761 cattaagcct catagcaaag ataacattgt ttttttttat acttttgcaa aagtgggaaa
     8821 aattggtttt ccagcactga aacttatagg tggaaccatg gaagtccatg tttaacctga
     8881 accagattat gagatgttct gtataacaat aagataacta aattttagca gatgaataga
     8941 gttttagtag ccactggagg cggcgattgt cctgggttaa atgcagtgat aagggcggtt
     9001 gtaaagcgag cctctcagga acccgactgg gaggttattg gaagcattca gtcttttgat
     9061 ggaatacttc gtgagccaac agaaataaga gtattggacg aaaaggcagt atcagggatt
     9121 catgttcaag gaggaactat tatcggtaca accaataagg gaggcccatt tgcatggcct
     9181 attaaaaaca aagatggttc ttggggtgct gttgaccgtt ccgatgaaat gattaggaag
     9241 ctgcagtatt taggtattga tgctgtaata agtataggag gtgatggttc gcagaaaata
     9301 tcgcagcaac tgtatgaaaa ggggctaaat attattggtg ttcccaaaac catagataac
     9361 gacctttcgg ccaccgattt caccttcgga tttcaaactg ccgttcaaat tgctacagat
     9421 gcggttgata agctggttac aactgcctcg agtcataaca gggtgcttat tcttgaggtt
     9481 atgggtcgct ttgccggatg gatagccctt catgcagcca tagcaggagg agcagaggtt
     9541 tgccttattc cagagattcc ctatgacatc aataaggttg cagagaagct taacagccgc
     9601 tataataagg ggaaaggtca cgccattgtg gttattgccg aaggggcaaa accaataggc
     9661 ggcactctaa cttcggagca gagcgatgag gtgggataca acaacctacg tcttggtgga
     9721 gtagcccact cgctaactaa cgatttgaaa aggattggct ttgaggcaga tattagggaa
     9781 acagtgcttg gacatcttca aagaggagga atcccagtag catacgatcg tgtactggca
     9841 acccagtttg gggttaaagc ttttgaaatg gtgcttcagg gaaaatttgg tgaaatggta
     9901 gcctaccgtc accccgacat tatttcggta ccccttattg acgctattaa ccgtccaaac
     9961 tacgttgatc ccaactgcga tttggttcgc actgccaggg gtgttggtat tagttttgga
    10021 gattaatttg aattataaca gcaacagtaa aaggaacttt ggctaagttc ctttttttct
    10081 ttatgttcta catgaagaat aaaaattatc tagttgttgc aaacctcgca ggaaaaggta
    10141 gtattaatgc ctgatatttc gatcaactcg taaccagcct ccttcatatc ctcatcgtca
    10201 ggctcaaact gccaggtaat gtttaccttg tatcccgttt caacaaactg tttaaatttc
    10261 tgcataagcg taaccagctg cttgaaggta gcggagttga tgtacttgaa aaataaccta
    10321 acgtttatgg gtttcatccg gatggaatcg gtccttccca attctacaaa ataagcttcg
    10381 agccatgaaa atattggctc aaaaaaagta gccacattct cggggtggga aataccggta
    10441 atggagaaat ttccattttt ggggtcaaag ataacctccg gtgaatcctt tttaggttct
    10501 atatataact tttccatttt aaattagttc caggctagct gttttttgga ataaaatcaa
    10561 aaggaatgtt aagaatttcg gccagctcct cacctgcttc ctccatttca gaatcatgag
    10621 aagggaagca ccaggtaacc cgaacgtttt tgccaagtag ggataaatct ttaagcttgc
    10681 tgagcaggag gagtatcatc tttgatgagg ccgtgttgaa gtagtcaaaa tccagaacca
    10741 ggttagtggt atcatttggg ctatttctgt actcgtccca ccactgaagc aaaggcatgt
    10801 aaaatgcctt cacatcctct ggaagtgatt ttccctgaat tttgaaaatt ccctgctgct
    10861 ggtcaaattc tacaagaggt gtaactgctg aaggctcatt aaaaaaacgt tcaaccataa
    10921 tattttaccc gttctacttc agtaatgttt ggtagccaaa aatcttaaat ggtaaatcga
    10981 gcatatcggc ataatcgttg cctgcatcca tcatgtcctc gtcatcctcc tggtagtacc
    11041 attcgatagc tgcgtttcgc cctgacaaat gtagcttatt gagcagttca aagagctcca
    11101 gtatcatttt ggatgaggcc gtattgaagt actccattcg taccttcaat gttgttgcgt
    11161 cgttaggaga ttgacagtac tcggtaagcc acttaattat cggatggtaa aattccttca
    11221 catcttcagg cagcgattta cctgaaattt caaacgtgtt tcccgttgaa tcaaaactca
    11281 cagtaggcgt ttcagcagta cccttaatat aaaacttatc cattattcta gtatttatca
    11341 ccaacaaaaa aacttatcac tcctgtaaca aatatatgag gaaattttaa agaaatccaa
    11401 cttcaacaaa aaatttaaca ctcaaagtaa taccaaagta atgtaacgta cactttatca
    11461 gcaacatata tactatacgg aagacttatc ccaaaagtta ttggcaagta accgtggagt
    11521 atctacctgc tgctccccat aaaattcaat cctatcaatc agctctccta tcaaaatgaa
    11581 caaaatcatc aacatagaaa tgtgttggtt tgagaaaaat attgagccga ggggaataag
    11641 aattcccaga aatacccgaa tgaagctaaa aataaattct aatgaactta cagaatagtt
    11701 tgcatgtcga agtatgtata aggctgcctt gagaacaagc actaggacaa gtaggcgctc
    11761 aaagctgttg aaaattgaaa agattgtcag agctgtaatc acaacatttg cgctatgaac
    11821 atgaatttta tacttcttat cagtaacgga gtaaaccatc tcaacagcaa ctaaaagaag
    11881 caaccccaaa ataacagcaa tcactgatag aacgatgagg ttaaaaaaaa tcaggtttaa
    11941 tgctgacagc gaaaaaaaga gggtaaaaag caggatttca cggctcaacc aactactctt
    12001 aacattagca acggaacgta atgccctaaa gggcttgcct aggtggaagg tgctcagtag
    12061 agcccctaca attcctaaca gaataaacag ggttatcccc cttgaagcga tgcctaccca
    12121 tggcatcgaa cccacccacc caaccataag cgagcaaatt agcgtaaaga tggctaaagg
    12181 caactcatga atggggttaa ttttagaagg ggcaaaatgc ttcatccctg tatgttgcaa
    12241 ggggctatag cccgtaactt gttcgtcgat atgggggata tggtttacaa tatcctcgtt
    12301 tttagtaaca attctaggag aaaatggttt agcggaaatg ccgggtgcct gaatgctagc
    12361 tgcagtttca atgctgccaa atgagagagc acctgtaggg catgcttcag tacaggccgg
    12421 tatttctcca cttttcaacc tttcgttaca catggtgcac ttttcaacaa cccctcttaa
    12481 gggattatac tttggcgcat caaaggggca agcccatgtg cagtatttac aaccaatgca
    12541 tcttttgtta ctatgaagta tagccccagt ttcctgatcc cgatagtaag cagaagcggg
    12601 gcaggccttc aggcatgggg catcagtaca atggttacat gccattgata agctaacaaa
    12661 tccataaagg ggaacttttt cggggttcac accaaccacc tgcctccaca aaacaggtgg
    12721 ttgaactccg ttttcgttat aacaggctac cacacatgca tggcacccta cacatctggc
    12781 gtaatcgaat ataaaaccgt tgtactgcat tgcttacttc tttaccaggt taacctccac
    12841 cctgttatta tgaaaagcag ccccatgtcc catatcggtt tcgcgaccgg cagaaagcgt
    12901 gttgagtgca gctccctgag atttccacca accattataa atcaccacgc agccctttct
    12961 tacc
//
LOCUS       DAOPHX010000069        12923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_5694_length_12923_cov_5.603746, whole genome shotgun sequence.
ACCESSION   DAOPHX010000069 DAOPHX010000000
VERSION     DAOPHX010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 12923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12923
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5694_length_12923_cov_5.603746"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..904)
                     /locus_tag="PK502_06455"
     CDS             complement(179..904)
                     /locus_tag="PK502_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPH00492.1"
                     /translation="MKAMILAAGYGTRLLPLTQNKPKALIEVGGKPLLEHCIENLINF
                     GFNEIVINTHYFASQVISFLNSKTFDANIFISDETERLLDTGGGIFKARQFLDGNEPF
                     LVHNVDIISNIDLAELYNIHGQADILATLAVNQRDSSRVFLFNNDMQLAGWRNMVTGK
                     VIISNFSPQLTAYAFCGIHVISPRIFSAIKATGVFSIVDIYLNLCKDYRIQGVNLPNN
                     FILDVGNPDSIAIAESFLHSCNH"
     gene            complement(1162..2610)
                     /locus_tag="PK502_06460"
     CDS             complement(1162..2610)
                     /locus_tag="PK502_06460"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPH00493.1"
                     /translation="MDNLIMMMHALEEQHAELAKLFRNTFSVEPLCFEPIPVSGSLRK
                     YYRLQNGNTSAVGTFSPDVDENRTFFKLTDCFTRQSLNVPQVLAISPCMRYYLQSDLG
                     STSLFDIITSPGKFPQHNLNNFLSIAVTSLVDFQVKGFNEISSSGQLQLPLFDRTSMM
                     WDMYYFKYCFLKPSGISFNEQLLESDFEWFAKELLEQKHFLFQYRDFQSRNIMVHNNQ
                     LYFIDYQGGRIGPPLYDIASFLYQARAKLDNDKRFELLSLYLDRLNCLLSIDKEELKA
                     KFPAMAMFRIMQTLGAYGFRGFFEKKTHFIQSIPLAVDNFINLSRSAPGPHTAYITDL
                     LEQYRVLFQPSSCFSPSGQELTVYITSFSFKNGYPEEHPEHGGGFVFDCRCLPNPGRM
                     GRYKNLTGLDFEVIQFLDESDEAQRFIANATALVKDAVENYCQRRFSYLSVAFGCTGG
                     QHRSVYCASKLALAIENTKNVRVVVQHREFPA"
     gene            complement(2603..3280)
                     /locus_tag="PK502_06465"
     CDS             complement(2603..3280)
                     /locus_tag="PK502_06465"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HPH00494.1"
                     /translation="MLGTIVNAVAILVGGVLGLVLKSRLPEKYTAVFFQVIGLFTLVL
                     GFSMSIKTEKPLLMVFSLILGGVSGIILNLQQRIDTWAIALKNRFKLKGDRFAEGMVV
                     AFLLFCMGSLTVLGALEEGTGGEPKLFYVKSLMDGFSALALASTLGVGVIFSILPLLV
                     YQGGLTWIAAATAKSLSNAMVNELSAVGGVLLIALGINILGIKKLEVVNLLPSLVFII
                     LLFYLFG"
     gene            3458..3916
                     /locus_tag="PK502_06470"
     CDS             3458..3916
                     /locus_tag="PK502_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00495.1"
                     /translation="MQLSLMRKDEIIDFIEFNITGDSENYNRCDDESLYLNTNVFNIF
                     ASCFERSNHLFDFISPTLYNSRNFIPLRNELLHNLETLLVIKNIAEFQNYMTGIFLGK
                     DLLKVLMDYGPNFERHWRGYMRKIIQVNRHLMRLIDQCIDEERVLWVIPY"
     gene            complement(3903..5000)
                     /locus_tag="PK502_06475"
     CDS             complement(3903..5000)
                     /locus_tag="PK502_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HPH00496.1"
                     /translation="MKLVADKDIPYLRGVLEPFFEVEYIPGAGISCDVVRDAEVLLVR
                     TRTRCNADLLQHSSVRFIGSATAGFDHIDSDYCSSNGILWRNAPGCNAGGVQQWVGAA
                     IVLWLETTQRLPQSLVLGVVGVGHTGSKVAELGKALGMKVLCCDPPRTRRENLPHFVN
                     LETILSSADIISFHVPLNTTGPDKTFHMLDSDSLQRCKPNSLLINASRGEVVDSHALT
                     AFLNANPFAHAAIDVWENEPNILNELLNRCMVGTPHIAGYSLEGKVAATQMVVDAVSQ
                     HYRLELHPWQPVPNPTLAKVKLNGISSLPEAISASYNIVGDDLRRISSDFEGYRNTYS
                     YRRDFSGFEVATSDPKLKQNLTRVGFTVSRG"
     gene            5164..5607
                     /locus_tag="PK502_06480"
     CDS             5164..5607
                     /locus_tag="PK502_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HPH00497.1"
                     /translation="MILSSLNEFGTYVNLHPLFSRIESALITMDFTHPGERIYIEGDN
                     LYATPSFSKAKAKEEALLEAHDRYIDVQVCLQGEEIMGWRNRSECLLPKEPYNEKDDI
                     TFFSDPPATFLKVPAYHFVIFYPSDCHAPLIGTGLIKKVVFKVKL"
     gene            complement(5756..6076)
                     /locus_tag="PK502_06485"
     CDS             complement(5756..6076)
                     /locus_tag="PK502_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00498.1"
                     /translation="MGKFSSKHVVEEIQGIRCTLVESGINKNRLDFLKSILEANGLKV
                     CFEAIPQAEGQEVLYKIGVTDLTFNPTIAIFGRRLKTPDGTVISPKYWLQETDSIKPY
                     YWLQ"
     gene            complement(6085..7509)
                     /locus_tag="PK502_06490"
     CDS             complement(6085..7509)
                     /locus_tag="PK502_06490"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HPH00499.1"
                     /translation="MLLQYLSLMRQEVSLIFLAVLVLICELTVNQRYKKVVIPITLVL
                     FGVHTILGFVPFDGGKLFGGMFATSHAQALMKSVLNVGVFIILLQSVAWLKKEVNFQK
                     SNEFLILLFSTLTGMYFMISSGDFLMFYIGLEIASIPMAMLAAFDTFRSKSSEAGIKF
                     LLNSALSSGILLFGISLFYAISGTIYFDDLPLYFSPVSPLFALGFIFFISGLAFKMSL
                     VPFHLWTADVYEGSPTIVTSYFSVISKGAAAFITSILLFKVFFNQLYLWKDIIYLLSV
                     LTMTVGNLFALRQTNIKRFLAFSSISQAGFILLGLVNVDSLGVTAIIYFILIYILSNL
                     SAFGVVMAIANVTGKEEIKDYNGLYKTNPLLSLTMMLAMFSLAGIPPTAGFFSKFFLF
                     TDAAKGGYYWLVFIAVLNTIISLYYYLLVVKAMFISPNEQPIERFRSDIYTRTALLIT
                     VVGIFAVGIYSPIFEYIRNIIDKF"
     gene            complement(7525..9024)
                     /locus_tag="PK502_06495"
     CDS             complement(7525..9024)
                     /locus_tag="PK502_06495"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HPH00500.1"
                     /translation="MNLSLFVIIPLLTVLLILFSKSIRQTRLIAAIGMSVQLVLSAIL
                     VVLYVSLRKHGNADQFVFVKDIEWFKSLNFNFTMGVDGISVALIGLTSIVIFAGVFAS
                     WKMEDLHREFFISLLVLAGGVFGFFITIDMFSMFVFYELAVIPMYILIGIWGTGKKEY
                     SAMKLTLMLMGGSALIIVGILGLYFHSGVSGGPLTMNLMNLSKVHFPVEVQMFLFPFL
                     FIGFGVLGALFPLHTWSPDGHASAPTAVSMLHAGVLMKLGSYGVFRIAIYLLPEAARQ
                     MGPFFMVLATIGIVYGAFGAIMQKDLKYINAYSSVSHCGLILFGLLMMNKTAMDGAIL
                     QMISHGIMTALFFALIGMIYGRTHTRLINEMGGLLKVMPFLGAAYVIAGLASLGLPGF
                     SGFVAEMLVFFGSFQHADLFHRILTILAASSIVITAVYILRVIGIFLLGPVKNEHHLL
                     LTDATWWERLSAGTLILFITIIGFYPYFLTNDLIGNSLEPILQKILMFN"
     gene            complement(9037..10965)
                     /gene="nuoL"
                     /locus_tag="PK502_06500"
     CDS             complement(9037..10965)
                     /gene="nuoL"
                     /locus_tag="PK502_06500"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HPH00501.1"
                     /translation="MQMSFQFTILIPLIPLISFLIIGFLYSKMSNKLAGIWGSVSIFS
                     SFLLSLFTAYRYFFEVGRVNGVYPKILGFDFEWLRFSDSLSAHLGTLIDPISVMMLVV
                     VTTVSLMVHIYSIGYMKGDRGFNRFFAFLSLFSFSMLGLVLSTNIFEIYIFWELVGVS
                     SYLLIGFYYEKNSAVAASKKAFIVTRFADLFFLIGILILSFYTKTFDFISLTDPSNIA
                     VMQTGSITFMGLSVTTWALLLIFIGGAGKSAMFPLHIWLPDAMEGPSPVSALIHAATM
                     VVAGVFLVARLFPIYALGSDQALQVIGYVGAFTSLFAAVIAMTQMDFKRVLAFSTISQ
                     IGYMMVGLGVSGYGNEAGLGYMASMFHLFTHAMFKALLFLCAGSVIHVVHTNFINEMG
                     GLRKHMKITHITFLIATLAIAGFPGFSGFFSKDEIITAAYHNNKIIFYTELLVAGITA
                     FYMFRLYFRVFWNQEASYEHKPHEVEKTMTIPLIILAFLSIAVGYIPFGNFISSDSMP
                     LESHINWGVASASIIVSFTGIIIAWVLYKKPSNLPERIVSSIKVLYTWAYHKFYIDEV
                     YLFVTKKIIFNLISRPIAWFDRHVVDGAMNGIAWTTSWVSESIKGLQSGQLQKYALAF
                     VSSTIILSALLIYILLNV"
     gene            complement(10971..11288)
                     /gene="nuoK"
                     /locus_tag="PK502_06505"
     CDS             complement(10971..11288)
                     /gene="nuoK"
                     /locus_tag="PK502_06505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPH00502.1"
                     /translation="MMGGLALQDFLVVSTSMFFIGIFGFLTRRNLITILMSVELILNS
                     VNINFVAFNYFLHPEAMHGIFFAIFVIAVAAAEAAVAIAIIINIYRRFKSINIENVSE
                     MKY"
     gene            complement(11290..11844)
                     /locus_tag="PK502_06510"
     CDS             complement(11290..11844)
                     /locus_tag="PK502_06510"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPH00503.1"
                     /translation="MEIAASKVIFYIIAAAILFFALLSVTSKRILRAATYLFIVLIST
                     AALYFLLNYQFLGAVQIALYAGGIVVMIIFSILLTSHAGQKFEPVSRLKFFASLLAVL
                     VGWALSLWVILSHPFVASSKEAVEVNMDSIGKALLSYDRNGYVLVFEVISILLLAAMI
                     GAIVISKKQPGDGETNPDNSKTSK"
     gene            complement(11847..12323)
                     /locus_tag="PK502_06515"
     CDS             complement(11847..12323)
                     /locus_tag="PK502_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPH00504.1"
                     /translation="MGLKSYLSGLFKGFYSLLVGLRVTNSYFFHPSRIVTQQYPENRK
                     TLKMFERFKGEVILVHNENNEHRCTGCGICEMNCPNGSIEIISKMETGEDGKKRKAID
                     KHIYHLSMCTFCGLCVKSCPSKALAFGNEFEHAVFDRAKLDKVLNKEGSVLMKGVE"
     gene            complement(12335..>12923)
                     /locus_tag="PK502_06520"
     CDS             complement(12335..>12923)
                     /locus_tag="PK502_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPH00505.1"
                     /translation="SGAQIVSYELSVGLSLVTMVILTGTMQLSQIIELQSDGWFIFKG
                     HIPALISLIIMMIASTAEINRGPFDLAEAESELTAGYHTEYSGIKFAFFYLSEYMNMF
                     VVSALVATVFFGGWMPLHIGSWQAFNHIMDFIPPIIWFILKSFALMYVIMLFKWTFPR
                     LRIDQLLKLEWQYLLPISLFNVLLMAFVVYMKWYF"
BASE COUNT         4054 a         2838 c         2819 g         3212 t
ORIGIN      
        1 ggctgcaaat ataccacctc tcctaaatcc atccaaactt ttttacacct tttttttaag
       61 ttttcaacta caattcttta ttacaacatt accttgctga ttaacaatta catcatgtca
      121 ttcaaaatat tcagaaaaac taaaatttct caggtaaaaa gtgcagcagt taatctggtt
      181 aatggttgca tgaatgcagg aaactttcgg ctatcgcaat ggaatcggga tttcccacat
      241 ctaaaataaa gttatttggc aagtttaccc cctgtattct gtaatcctta caaagattta
      301 ggtaaatgtc aactatagaa aatactcctg ttgcctttat agcgctaaat atccggggag
      361 aaataacatg gattccgcaa aacgcatagg cggtaagctg ggggctgaaa tttgaaataa
      421 tgaccttacc tgtaaccata ttcctccaac cggccagctg catatcattg ttgaagagga
      481 acacccttga ggaatcgcgt tggttaacgg ccagggttgc caggatgtcg gcttgcccat
      541 gaatgttgta aagttcagcc agatcaatat tggaaattat atctacatta tgcaccaaaa
      601 aaggctcgtt accatcaagg aattggcgtg ccttgaaaat tccaccaccg gtatcgagca
      661 agcgctcggt ctcatctgaa ataaaaatat tggcatcgaa tgtcttgctg ttaaggaaac
      721 taataacctg tgaggcaaag taatgagtat tgataacaat ttcgttgaag ccaaaattta
      781 tgaggttttc tatacagtgc tccaggagag gcttacctcc tacctcaatc agcgctttag
      841 gtttgttttg ggttaggggt aataggcggg ttccgtaacc tgcagcaaga atcattgctt
      901 tcatactagt tcaaaaatta gagaaaaacc aacttaaaaa acatcatttc gcaaaagctt
      961 agctaggaca aagctagaaa agcagtgaaa atcgacattt aggttttgtt aaagtaagca
     1021 attctattta aaagttgaca agatagaaat ctaaaaagta gaaatttata agtaggcgtt
     1081 aaaaacagaa ttttttttga ctgatttaca ggtgagtatg cgcttgctat gcatgaaata
     1141 aaagacaaaa tgtaacaagc attaagctgg aaactcccta tgctgaacga ctacccttac
     1201 gttcttggtg ttttcgatgg caagggctag cttactggcg cagtacaccg aacgatgttg
     1261 cccaccggtg cagccaaaag caacagacag gtaggagaaa cgtcgttggc aataattctc
     1321 aactgcatcc tttaccaagg cagtagcatt ggcaataaat ctctgagcct catcgctctc
     1381 atcaaggaat tgaattactt cgaaatcaag tcctgtaagg tttttatatc tacccattcg
     1441 accagggtta ggcaaacacc tgcaatcgaa cacaaagcct cctccatgct ccgggtgctc
     1501 ctcaggatag ccatttttaa aagagaagct ggtaatatat actgtaagct cctgacccga
     1561 tggtgaaaag caggatgagg gttgaaataa aactctatac tgttctaaaa ggtcggtaat
     1621 ataagctgta tgaggcccag gagcggagcg agagaggttg ataaagttgt caacagccag
     1681 gggtatgctt tggataaaat gtgttttctt ctcaaaaaag cctctaaaac cataagcacc
     1741 tagagtttgc atgatacgaa acatggccat tgctggaaat ttagccttta attcttcctt
     1801 atctatagag agaaggcaat ttaacctgtc taagtaaagg cttagcagtt cgaaccgttt
     1861 atcattatcc agctttgctc ttgcctggta taaaaaggag gctatatcat aaagtggtgg
     1921 cccgattctc ccaccctgat agtctataaa gtacagctgg ttgttgtgca ccataatatt
     1981 acgcgactgg aaatcacggt actgaaatag aaagtgcttt tgctctagca gctccttggc
     2041 aaaccactca aaatcggatt cgagtagctg ttcgttgaat gaaattccag agggcttgag
     2101 gaagcagtat ttaaagtagt acatatccca catcatcgag gtgcggtcaa acaaggggag
     2161 ttgaagttga ccggaagagc taatctcatt aaaaccttta acttgaaagt caacaaggct
     2221 ggtaaccgca atggatagaa aattgttaag gttgtgctgg ggaaacttac cgggagaagt
     2281 aataatgtcg aaaagggatg tggagcccaa atctgattgc aggtagtagc gcatacaggg
     2341 tgaaatagcc aatacttgag gtacattaag ggattgcctt gtgaagcaat cggtaagctt
     2401 gaaaaaagtg cggttttcgt caacatcagg gctgaaagtt ccaactgctg acgtgttgcc
     2461 gttctgtaag cggtagtatt ttcgaaggga acctgaaaca ggaattggtt cgaagcagag
     2521 aggttcaacg ctaaaggtgt ttctgaagag ttttgccagc tcagcatgct gttcttctaa
     2581 agcgtgcatc atcataatca aattatccaa acaggtaaaa taaaaggata ataaaaacca
     2641 gggaaggtaa aaggttgact acctccagct ttttaattcc aagaatattg attccaagtg
     2701 caataagcag tactccacca actgcagaaa gttcattaac catggcattg gatagagatt
     2761 tggcagtggc tgcagctatc catgtaagac cgccttggta aaccaacaag ggtaatattg
     2821 aaaagataac gccaactccc agggttgatg caagcgctaa ggcagaaaaa ccatccatca
     2881 agcttttaac gtagaaaagt tttggctctc cgccggttcc ctcttcgagt gcacccagaa
     2941 cagtcaaaga gcccatgcag aaaagcagaa aagcaacaac cattccctcg gcaaacctat
     3001 cgcctttgag cttaaacctg tttttaagag ctattgccca tgtatcaatt cgctgctgta
     3061 ggtttaggat tatccccgaa acacctccta gaattaagct gaaaaccatc agcagtggtt
     3121 tctcggtttt aatgctcatg gaaaatccca aaacaagagt aaacaagcct atcacctgaa
     3181 aaaaaactgc ggtatatttt tcaggtagcc gcgacttcag caccaagccc agcactcctc
     3241 ctacgagtat ggccacggca ttaactatag ttccaagcat attatactaa tccttacaaa
     3301 tttcattgct aaaatacaca ttgcctgttg atgtatgggt taatatcgtc caaaaattgt
     3361 ttgaaattca ataaaatatt aattttgaca ttaaatgagc agttgtgaaa atcggaatag
     3421 gcaacggttc tactagtgga gacaaactaa tgtcaaaatg cagctaagct tgatgcgcaa
     3481 ggatgagatc atcgatttca tcgaattcaa cattacagga gactcggaaa actacaacag
     3541 gtgcgacgat gagtcgcttt acctgaatac aaatgttttc aacatatttg ccagttgctt
     3601 cgagcggagc aaccaccttt ttgatttcat atcgcccacc ctttacaaca gccgcaattt
     3661 cataccttta cgaaacgagc tgctacataa cctggaaacc ttgcttgtta taaagaacat
     3721 tgccgagttc caaaactaca tgaccggcat ttttctgggt aaagacttac tgaaggtgct
     3781 aatggactac ggtccaaact ttgagcggca ctggaggggc tacatgcgaa agataatcca
     3841 ggtgaaccgc cacctaatgc ggctaataga ccagtgcatt gacgaggagc gggtactttg
     3901 ggttatcccc tactaacagt aaatcccacc ctcgtcagat tttgctttag cttaggatcg
     3961 ctagtggcta cctcaaaacc gctgaagtcg cgccggtagg agtatgtatt cctgtagcct
     4021 tcaaaatctg aactaattct cctcaaatcg tcgccgacta tattatagct ggcgctaatg
     4081 gcttcgggga gactcgaaat accattaagc ttaaccttag ccagggtggg atttggtacg
     4141 ggctgccatg gatgcagttc tagccgataa tgttggctaa ccgcatcaac caccatttgg
     4201 gtggcagcta ccttaccctc cagggagtac cctgcaatat ggggtgtgcc caccatgcaa
     4261 cgattcagca attcgtttag aatgttcggt tcgttttccc atacatcaat agcagcatgg
     4321 gcaaaggggt tggcatttaa aaaggcagtt aaagcatgag aatccaccac ctcgcccctt
     4381 gaggcattta taagaaggct gtttggcttg catctctgta aggaatcact atcaagcatg
     4441 tggaaggttt tatcgggccc ggtagtattc aacggaacat ggaatgagat gatgtcagcg
     4501 cttgacaaaa tggtttccag gttgacaaaa tggggcaaat tctccctacg agttcgtggc
     4561 gggtcgcagc atagaacttt cattcccagt gctttgccaa gttcagccac cttgcttccg
     4621 gtatggccaa ctcccaccac gcccaaaaca agactttgag gcaacctttg tgtcgtttcc
     4681 aaccataaaa caatggctgc gccaacccac tgctgcacac caccggcatt gcatcccgga
     4741 gcattgcgcc aaagtatccc atttgaactg cagtagtcag aatcaatatg gtcaaatccg
     4801 gctgttgccg aaccgataaa tcttacgctc gaatgttgaa gcaaatctgc attgcaccgt
     4861 gtacgggttc gaacaagcaa cacctcggca tccctaacaa catcgcagct aattccagct
     4921 ccgggaatat actctacttc aaaaaagggc tccaggactc ctctcaggta aggaatatcc
     4981 ttatcggcta ccagcttcat agctccagat tttcattcag caggcaaaga tagctaaatg
     5041 gaatttccgg ttgggccatc aaaattttgc tcatttttag ctgtgataat cacgattgtt
     5101 taccttagct aacaattaca ggaacttttc atctaaacaa aacattgtta attccgacct
     5161 gtcatgattc tatcgagcct aaacgagttt ggcacatacg ttaacctaca tccacttttc
     5221 tccagaattg aaagcgcctt aataacgatg gactttacac atcccggcga gaggatatac
     5281 attgaggggg ataatttgta tgcaacaccc tcgttcagca aggctaaagc aaaggaagaa
     5341 gccttacttg aggctcacga caggtacata gacgtgcagg tttgcctaca gggtgaagag
     5401 attatgggtt ggcgaaaccg ttcggaatgc ttactaccca aagagccata caacgaaaag
     5461 gatgatatta ctttcttttc cgatccacct gcaaccttcc tgaaagtgcc tgcctaccac
     5521 tttgtaattt tttatccgtc cgattgccac gccccactga taggcacagg gttaattaaa
     5581 aaagtagtgt tcaaggtaaa gctataacct caactggaaa atccctgcaa ctggctatcc
     5641 aaaacaggcc aacagaatct aaggataacc atgaatgatg aaagattacc ggataacttg
     5701 caaatccatg aggaatttag gggtttgcag gttaacggtg ctatggaaag tctggttact
     5761 gcaaccagta gtaaggcttt atcgaatccg tttcctgaag ccagtacttt ggagagatta
     5821 ccgtgccatc gggagttttc aaccgacgtc caaaaatggc gatagtagga ttaaatgtta
     5881 agtcggttac gccaatcttg tagagtactt cctgaccctc agcctgggga atggcctcga
     5941 agcaaacctt cagcccattt gcttccagaa tacttttaag aaagtctaac ctattcttgt
     6001 tgatacccga ctcaaccaga gtgcaacgga taccctgaat ttcctcaaca acatgctttg
     6061 agctaaattt acccatagcc gtatttaaaa tttatcaata atgtttcgta tgtattcgaa
     6121 tatcgggcta tagatgccga cagcaaaaat tcccacaacc gtgattagca gcgcagtccg
     6181 ggtataaata tcgctcctga agcgttcaat tggctgttcg ttagggctaa taaacatggc
     6241 cttaaccacc aggaggtagt agtagagcga aatgatggta tttagcacgg caataaaaac
     6301 aagccagtag tagccaccct ttgccgcatc agtaaaaagg aaaaatttgc tgaaaaagcc
     6361 agccgttggc gggatgccgg caagcgagaa catggcaagc atcatggtta gcgatagcaa
     6421 cgggttggtt ttgtataagc cgttgtagtc ctttatttcc tccttccctg taacatttgc
     6481 aatagccatt accacgccaa atgcggatag gttggaaagt atgtagatca aaataaagta
     6541 gataatagct gtaactccaa gtgaatcaac gtttaccaga ccaagaagaa taaagccggc
     6601 ctgggagatt gatgagaatg ccagaaatct tttgatattg gtttgccgga gggcaaacag
     6661 gttaccaacg gtcattgtga gcaccgagag caggtaaatg atatccttcc aaaggtaaag
     6721 ctggttgaaa aaaaccttaa agagcagtat ggaggtgata aatgcagccg cacccttaga
     6781 aataacggag aaatatgagg ttacaatggt gggagagccc tcgtaaacat cggctgtcca
     6841 aagatggaat gggactagcg acatcttaaa agccaagcca gaaatgaaga atatgaatcc
     6901 cagggcaaag aggggtgaaa caggcgaaaa gtacaacggc aggtcatcaa aataaatggt
     6961 tccactgatg gcataaaaca aggaaatccc gaacagcaaa atacccgacg acaaagccga
     7021 gtttaggaga aactttattc ccgcctccga tgatttgctc ctgaaagtat caaaagcagc
     7081 caacatggcc ataggtattg atgcaatttc aagtccaatg tagaacatca ggaaatcgcc
     7141 tgatgaaatc ataaagtaca tcccggtaag ggtggaaaac aacaggatta ggaactcgtt
     7201 gcttttctga aaatttactt ccttcttcag ccatgctact gattggagta gaataataaa
     7261 aacgcctacg ttaagtacgc tcttcatcag ggcttgggca tggctggtgg caaacattcc
     7321 gccgaacagc ttacccccat cgaaaggaac gaatcccaaa atggtgtgca cgccaaataa
     7381 tacgagggtg atggggataa caactttttt atatcgttgg tttacagtta attcacatat
     7441 caatactaac acagctagaa aaatcagcga tacctcctga cgcattaggc taagatattg
     7501 taatagcata atttaggtta tcagttagtt aaacatcaaa attttttgta gaatgggttc
     7561 caagctatta ccaatgaggt cgttggtaag aaaataggga taaaaaccta ttatagtgat
     7621 aaaaagaatc aaggtaccag ccgaaagccg ttcccaccag gttgcatcgg taagcagcag
     7681 gtggtgttcg tttttgacag ggccaagcag gaaaattccg ataaccctta agatgtaaac
     7741 agccgtaatg acaatggagc tagcagcaag aatggtcagg attctatgaa acaaatcggc
     7801 atgctggaac gagccaaaga atacgagcat ttcggcaaca aagccgctaa agccaggaag
     7861 tccaagcgag gcaagacctg caattacata agcggcgcca agaaatggca tcacctttaa
     7921 aagcccaccc atctcgttga tcaacctcgt gtgggtgcgg ccgtatatca tcccgataag
     7981 ggcaaagaag agggcggtca taattccatg ggagatcatc tggaggatag cgccatccat
     8041 ggccgtcttg ttcatcatca gcaatccaaa aaggatgagt ccgcagtggc taacggacga
     8101 gtaagcgtta atgtacttta agtccttttg catgatggcc ccaaaggctc cgtacacgat
     8161 tccgattgtt gccagcacca taaaaaatgg tcccatttgc ctggctgctt cgggtaggag
     8221 ataaattgcg atacgaaaaa cgccgtaact tccgagcttc ataagaactc cggcatgaag
     8281 catagaaaca gcggttggtg ccgaggcatg tccatcgggc gaccatgtat gcaggggaaa
     8341 taatgcaccc aaaacgccaa aaccaataaa gaggaaagga aacaggaaca tctgaacctc
     8401 aacgggaaaa tgcactttgg acaggttcat caggttcatg gttagcgggc cgccgcttac
     8461 acccgaatgg aagtatagac ccaaaatacc cacaatgata agtgcagaac ctcccatgag
     8521 catcagggtg agcttcatgg ccgagtactc cttcttgccg gttccccaaa taccaattaa
     8581 aatgtacatg ggtatcaccg ccaactcgta aaaaacaaac atggagaaca tgtcgatggt
     8641 gatgaagaag ccaaacaccc cacccgcaag cacgagtagc gagatgaaaa actcgcgatg
     8701 caggtcttcc atcttccacg aggcaaacac accggcaaaa atgactattg aggtaaggcc
     8761 gattagggca accgaaatgc cgtcaacccc catggtgaag ttgaaattta agcttttaaa
     8821 ccactcgata tcctttacaa acacaaattg atcggcattc ccatgttttc gcaggctaac
     8881 atacagcaca accagtattg cgctaagcac cagctgaaca gacattccga tagccgctat
     8941 caaccgtgtt tgccttatac tcttagaaaa aagaataagc aaaacggtta gtagcgggat
     9001 gattacaaaa agcgaaaggt tcatattctc accatgttag acgttcaaca aaatgtagat
     9061 aagcaatgcg cttaaaataa tggtgcttga aacgaatgca agagcgtatt tttgaagttg
     9121 tcccgattgc aaacccttaa tactttcgga aacccagctg gtagtccatg ctatgccatt
     9181 cattgccccg tcaaccacat gcctgtcgaa ccaggcaatt ggccttgaaa tgaggttaaa
     9241 aatgattttt ttggttacaa aaagatatac ctcatcaatg taaaacttat ggtaggccca
     9301 ggtgtagagc accttgatgc tgctgacaat tctttcgggc aggttggagg gtttcttgta
     9361 aagcacccat gcaataataa ttccggtaaa gcttacaata atggaagcag atgcaactcc
     9421 ccaatttata tgactttcca ggggcatact gtcagaagaa ataaaattac caaagggtat
     9481 gtagccaaca gcaatggaga gaaaggctag aataatcaag ggaatggtca tggttttctc
     9541 cacttcatgt ggcttatgct catagctagc ttcctggttc cagaaaaccc taaagtacaa
     9601 ccggaacata tagaaggcag taattcctgc caccagcaat tccgtgtaaa aaataatctt
     9661 gttgttatgg tatgcagcag taatgatttc atccttgctg aagaatcctg agaagcccgg
     9721 gaatccggca attgctagag ttgcaattaa aaatgtaata tgggttatct tcatgtgctt
     9781 tcgcagcccg cccatttcat tgataaagtt ggtgtggaca acatgaataa ccgatccggc
     9841 acaaaggaac aaaagcgctt tgaacatggc gtgggtaaac aaatggaaca ttgatgccat
     9901 gtagcccagg cctgcttcgt ttccgtagcc cgaaactccc aatcccacca tcatatagcc
     9961 aatttgggat atggtggaga aagctaaaac gcgcttaaag tccatttgtg tcatggcaat
    10021 aacggctgcg aacagggagg tgaaggcgcc cacgtagcca ataacctgaa gtgcttgatc
    10081 agaacctaaa gcataaatcg ggaaaagccg ggctaccagg aatacacccg caacaaccat
    10141 ggtcgccgca tgaattaggg ccgacaccgg ggatggcccc tccatggcat cgggaagcca
    10201 gatatgaagc ggaaacatgg ccgacttacc tgctcctcca atgaaaataa gcagcaatgc
    10261 ccaagtggtt acgcttagcc ccataaaggt tatacttccg gtttgcatta ccgcaatgtt
    10321 gctggggtcg gttagcgaaa tgaaatcgaa cgtcttggtg taaaacgata ggattagaat
    10381 tcctattaaa aagaagaggt cagcaaatcg ggttacgatg aaggctttct ttgaagccgc
    10441 taccgcggaa ttcttttcat agtaaaatcc aattagaagg taggacgaaa caccaaccag
    10501 ctcccagaaa atgtatatct caaaaatatt ggttgataga actaacccca gcatggaaaa
    10561 gctgaatagc gagaggaaag caaaaaagcg gttaaatccc ctgtcgccct tcatgtagcc
    10621 gatgctgtag atatgaacca tcagcgaaac agtggtaacc accaccagca tcatcaccga
    10681 aatggggtca atcagtgtgc ccaggtgggc cgacagcgag tcgctaaatc ttagccactc
    10741 aaaatcgaac cctaaaattt tagggtacac cccgttgacc ctgccaacct caaaaaaata
    10801 tctataggcc gtaaacaacg acaggaggaa cgatgaaaaa atagaaactg aaccccatat
    10861 cccagccagc ttattggaca ttttactgta tagaaatcct ataatcaaaa agctgataag
    10921 cggaataagc gggattagta tggtaaactg gaagctcatc tgcatagtgg ttagtatttc
    10981 atttcactta cattttcaat attgatggac ttaaatcggc ggtaaatgtt gataataatg
    11041 gcaatggcaa ctgcagcctc agctgctgca acggcaataa caaatatggc aaagaagatt
    11101 ccgtgcatgg cctcggggtg cagaaagtag ttgaaggcca caaagttgat gtttaccgag
    11161 ttaagtataa gctcaacgga cattaaaatg gtaatcaggt tgcgtcgggt aagaaagcca
    11221 aaaattccaa taaagaacat ggatgtgctg acaaccagaa aatcttgtaa agctaaacct
    11281 cccatcatat catttgcttg ttttagagtt atccggattg gtttccccat caccgggttg
    11341 tttttttgaa ataacaatcg ccccaatcat tgcagccagc aggagaatag aaataacctc
    11401 aaataccaaa acatacccgt ttctatcgta gctaagcaag gctttcccga ttgagtccat
    11461 gttcacttca accgcttcct ttgaacttgc cacaaaaggg tggctcagaa ttacccagag
    11521 cgacaaggcc catcctacca aaacggccaa caacgacgca aaaaatttaa gacggcttac
    11581 cggctcgaat ttctgaccgg catggctggt caaaagaatg gagaaaataa tcatcaccac
    11641 aataccaccg gcatataaag caatctgcac ggctcccaga aactggtagt tcagcagaaa
    11701 gtacagagct gctgtggata tcagtacgat aaataggtag gtggcagccc gcagaatccg
    11761 cttgctggta accgagagca gcgcaaaaaa aagtatggcc gcagcaatga tgtagaatat
    11821 tacttttgag gcagctattt ccatggctat tcaactcctt tcattaaaac agaaccttcc
    11881 ttattcagaa ccttgtcgag ctttgccctg tcgaaaaccg catgttcaaa ttcgttgcca
    11941 aaggcaagcg cctttgatgg acagctctta acgcacaagc cgcaaaaggt gcacatgctc
    12001 aggtggtaaa tgtgcttgtc gatcgccttt cgctttttcc catcctcacc cgtttccatt
    12061 ttcgatatga tttcgatgga gccgttgggg cagttcatct cacatattcc gcaaccggtg
    12121 catctatgct cattattctc gttgtgcaca agtataacct cacccttaaa gcgctcgaac
    12181 attttaagag tcttcctgtt ctcggggtac tgctgggtta caattctgct ggggtgaaaa
    12241 aagtaggagt tggtaacccg caggcctact aaaagcgaat agaatccttt gaataatccc
    12301 gagaggtatg attttaatcc cataatttac aagtttaaaa gtaccatttc atgtacacca
    12361 caaacgccat cagcagaacg ttgaacaggc taatcgggag caggtactgc cattcgagct
    12421 taagcagctg atcaattcta agccttggga atgtccactt aaacagcata ataacataca
    12481 ttagggcgaa tgatttcagt ataaaccaga tgatgggtgg tataaagtcc attatatggt
    12541 taaacgcctg ccatgagccg atgtgtagtg gcatccatcc tccaaaaaat accgtggcaa
    12601 ctagcgccga cacaacaaac atgttcatgt attcagagag gtaaaagaag gcaaatttaa
    12661 tgcccgagta ctcggtgtgg taaccggcgg taagctccga ttcggcttca gccaggtcga
    12721 aaggccccct gttgatttcg gcggtgctgg caatcatcat gattattaag gatattaggg
    12781 cgggtatgtg gcccttaaat ataaaccacc catcgctttg cagctcaata atctgtgata
    12841 gctgcattgt tccggtcagg ataaccatgg taaccagcga gagccccacc gatagctcgt
    12901 agctaacaat ttgagccccg ctt
//
LOCUS       DAOPHX010000070        12762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_5793_length_12762_cov_4.814590, whole genome shotgun sequence.
ACCESSION   DAOPHX010000070 DAOPHX010000000
VERSION     DAOPHX010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 12762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12762
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5793_length_12762_cov_4.814590"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>342)
                     /locus_tag="PK502_06525"
     CDS             complement(<1..>342)
                     /locus_tag="PK502_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00506.1"
                     /translation="DCLAFNSDKAKGELLRKSINGNLLLSSEFLTTGINFGWHHGYYS
                     AGVAQKIHATFPDATIIIFIRRQQSLICSAYQQYIKNGGTYGFKRWLYSGEVFSPEHL
                     CFDKLIDYYDKL"
     assembly_gap    343..352
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1176..2501
                     /locus_tag="PK502_06530"
     CDS             1176..2501
                     /locus_tag="PK502_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HPH00507.1"
                     /translation="MGSPEGDLPEAIEIDNNENIYLTGRFQGVCDFGSGLYLTSSGGF
                     DSFISKYNSSGEIQWVTNTAYGTAEDRTNSITLGFDGNLYTTGLSKSSTFYVGDGTIQ
                     DIYTNPDAKTDLFLASYTTSGTYRWSKQIPGNNNSSIFRAIACDEQNNLYVGGALAGT
                     LTIDGIDYTSAGNGDIILMKAHPTDGSAIWVRKGGSTADDQLNSIAVNDNFAYLIGYI
                     QGTGTIDSTATLQSSQFKTTGGNDIFLAKYNLEGRLLWKKTLGSTGADVGYGLNTINN
                     ILVATGYFSNTLNFNLSSITSGGGMDAAFFVFDVESNAVLAKSVSGTLEDRGQGVALD
                     SSYNAYLGGYFMSSTLSVGTTTLTNSASTFKDLFLAKYHNEFAAAFTNKKNVTCNGGS
                     NGELTVTPYFGTPPYTFTWKLNGNPYAETDSAITNLGVGTYEVTVTDYL"
     gene            2571..>5038
                     /locus_tag="PK502_06535"
     CDS             2571..>5038
                     /locus_tag="PK502_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00508.1"
                     /translation="MSCYGKQDGTINITVSGGTPSYTYFWTSSDGLGHVVDAEDQTGL
                     TAGTYSVEVTDHNGCMANGSYTITQPTRINITGEVTNITGSGSNGAVDITVSGGSGTY
                     SSYAWYFNDIPGGTHQDTTGINNGGDYKVVVTDNTACKDSNTFTVLDERIFHGWIVTK
                     TNVSCKGGSDGSATVSWAENTGTVTILWSPDGETTATISGKPAGSYTATLTDDMGTPG
                     DTGDDMTINVDVDLTEPAQPLGGGIVSYPVTCHGGSNGILDLTPSGGTLPYSYSWNTI
                     PAKTTQDINNLPAGNYEVTITDSKGCTTVVPGTVSEPDAISFSFAFTQPTCHGTIDGS
                     LAVSGLTGGTPGYSYLWSNTLTSSSISNIKSGSYWLKVTDSKGCYNTQSTYLDQPAAL
                     TISHVANQPTCPESKNGSIGITVGGGTSPYTYFWTGPDVANPTDEDLVNLGVGTYNVT
                     VTDANGCTKDYQVVLTEANPSPSAILSSSDTNNEICDGDNVVFTASGGSTYEFFLNGI
                     SVQGPGAGYAYNNSTLINNDEVFAEVTSSAGCKALTNTISTIVHTLPTVTANASPNPI
                     CEGDPVTLTGSGATSYLWDKGVTDGVPFYPTTTEIYTVTGTDDNGCTDTDQITVTVNP
                     VPDAQINTTDPTSYCEGETISTLLNASPSDGSYYQWIRNGANISGAYSNTYTASEAGF
                     YSFYVIKNGCMGISDEVEIIVHPLPSVSLADFEPVCADAEPFAITGGNPEGGIYSGTG
                     VTGGTFDPAAAGAGTHSITYFYTDANGCSASASADMVVNGLPVVSLADFDPVCLDAEP
                     FALSGGTPEGGIYSGTGVTGGTFDP"
     assembly_gap    5039..5138
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5139..6255
                     /locus_tag="PK502_06540"
     CDS             <5139..6255
                     /locus_tag="PK502_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPH00509.1"
                     /translation="TGVTGGTFDPAAAGAGTHTITYSYTDANGCSASASEDMVVNGLP
                     TANINTTDPTDWCEGTPINVNLTADVAESYQWLLDGQPITGETGQTLIATQAGTYSVA
                     ATTNGCNSTGNTITITVISTPSVAITTDDPTTWCAGDEVSVQLTAIPSGGDSYRWIKD
                     EVTIAGETQSNYTANEPGNYRVEALFAGGCSSVSEIFSLTENPVPTVSIATDTIQIDT
                     SSTYTFDAGAGFVSYLWSDTSTGQTLLVDGAVTGTGEFTYWVTVTNEFSCSASDTAVV
                     VVSPVGGVPMQISWEISIYPNPSSGEFKLKVSGIEPGTYTLTILNAGGQIVYGSTFSA
                     SDTEITRAINIQTLSKGLYYVRFENTNKGFTRKIILK"
     gene            complement(6311..7429)
                     /locus_tag="PK502_06545"
     CDS             complement(6311..7429)
                     /locus_tag="PK502_06545"
                     /inference="COORDINATES: protein motif:HMM:NF020874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HPH00510.1"
                     /translation="MKIAILGTRGIPNSYGGFERFAEVLSQILVARGHLVYVLSPTFN
                     GKGRSVTRLSSMLFLVNVGVGKHLPHNFQTLHYDLKSLCWANTEEIDVILECGHSFAP
                     WLPLFKKTFRQKIVVNLDGLEHKRSKWSKPAKLFLRFSEYMAVHFVARLVCDNQALVS
                     YYAASYGVKPKVIPYGAFPLGEIPPRDVLAMYGIFEPYYLMVSRLTPENNIEMIARSF
                     VALGENLIIIGRYDTRFGESLYRKFEHFKNIRFLGPNFNQDDLNALRYHSLAYIHGHT
                     VGGTNPSLLEAMACKCFVIAHDNPFNREVLGDEGWYFGSTTELNDRIREFGRLPAMSV
                     LKAKQSNYTRIKNLYSWEAVADSYEELFSSINSQQDQG"
     gene            complement(7664..8890)
                     /locus_tag="PK502_06550"
     CDS             complement(7664..8890)
                     /locus_tag="PK502_06550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPH00511.1"
                     /translation="MLFKAFNRGKDESLYAGYESLLRSIPLFMGVDSEGLGKIAKSLV
                     SKSIKAGEVLFNKGDYDFALYIVVSGQVKAHDGNHTFATFGPRQFFGEYSMLDSLARS
                     TTITALRDTELLRLDQGQFNLLLDKNPLLSRALLKTLVWRLRDYNVIEAELTAKNQEI
                     KRQKEEMDVQRKELVSLNQTKDKFFAIIAHDLKNPFGAVLSLSELLAREFESFEPEKL
                     HMFIQQIYKYSNNTFNLLENLLQWSLLQTGRTVVNLKSLNMAEIINENIDLLKANALQ
                     KGIELAWVNPDDHFVYCDRDMITTVLRNLISNAIKFTPAKGRIEVEVVKDSEYLKVMV
                     RDSGVGISPENQQKLFRIDSNPSTPGTNEEEGTGLGLILCKEFVEKNRGTIAVESTEG
                     KGSTFFFTVPRFENKS"
     gene            9446..11734
                     /locus_tag="PK502_06555"
     CDS             9446..11734
                     /locus_tag="PK502_06555"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPH00512.1"
                     /translation="MKLKLNIRQKILVYILGPSIVVFAGIFTYTTRTSRDIAYNQAIK
                     LTDSYAHQYALNIEGWLNQDLAITRTLAAAFLEYKQMPFEQWQRLIYPMYDRIILTTP
                     HIDAYWDSWEMSNLDPTWDKPYGRYFYIVYKENGVYKTKAELRSLTGDPDTYGAMKKA
                     AKEIINEPYVSALQRGQLMTTISSPLVENGKFIGLIGIDLLLTRFQNLVNGIKPYPNS
                     YAFLLSNKGNFIAHPDSTIYQKNIAERLPELNSRFSILSNVQKGKAFSFTQTGENGQR
                     LYYTFSPINIGNTQTPWSVGIAVPINDILYEANKNYNISIAILILGLILLIAIIFFVS
                     SSITRPIKSITHLLQDLANGKVDSTLAIKHDVHDEIGKMALALSESIKGINDKTEFAR
                     EIGSGNLKAEVSLLSEDDHLGKSLLEMRDNLQKAKEDEEKRKAEDEKKRWTNEGLAKF
                     GDILRQNNNNLSKLSDEIIKNLVWYLNASLGGIFVLNENRETGDKTFDLVAAFAYDRK
                     RILNKSYQFAEGLVGACAAEKDIIMLTEIPQEYIEITSGLGGTNPNFLLLTPLVNEDE
                     VMGVIEIASLRRFEEHEILFVKEIAKSIATTLHSVKVNSLTAELLMKSKEQAEIMAAQ
                     EEEMRQNMEELQATQEEAARKTFELEGLANALSASSLIMEYDPAGVITSINEKYSELL
                     SISREDIINKHYSDIATEAIDKEKLDKLWQEIRSGKTKYRKVVLNIKGKEHTLVETYT
                     PILDTQGRLYKVLKIATDITNL"
     gene            12136..12696
                     /locus_tag="PK502_06560"
     CDS             12136..12696
                     /locus_tag="PK502_06560"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPH00513.1"
                     /translation="MKKTLTVSLNGRSYELEEDAFKMLDEYLKQLESYFAEDAGKQEI
                     LSDIESRIADHFDESSRVRGVVIGADEVRRVIEIMGTPSDIADGEKVPKAEKPHRSYR
                     KLYRNMDDKVLGGVCSGMGCYWNVEPLLFRIIFIISIFWGGLGLLVYAILWIAVPPAL
                     TTTQKLEMRGEEVTAENIGKSFENKK"
BASE COUNT         3835 a         2904 c         2645 g         3268 t
ORIGIN      
        1 gagtttatca taataatcta taagcttatc aaaacacaaa tgttcagggg aaaaaacctc
       61 tccggaataa agccatcttt taaagccata tgttccacca ttcttaatat actgctggta
      121 agcagagcat atcagcgatt gctgacgacg tatgaaaatt attatggttg catctgggaa
      181 ggtggcatga attttttgag ctactccagc cgagtagtaa ccatggtgcc atccaaaatt
      241 aatacctgta gtaagaaact cagaactcaa taaaaggtta ccattaatgc ttttccttaa
      301 taactcacct ttcgctttat ccgaattaaa agcaagacaa tcnnnnnnnn nncaaaaata
      361 ctttgttaac ttccctgaat gactcgaaat ggtaattact aatagaaggg aaataaactt
      421 cctgaaacca tgtagttgcg gtttttggat aaccaatgtg aataattact ggcattattt
      481 tgaccaacat taaatttgaa tagacaaaaa tatcacattt atatatcatt taaagtaact
      541 tgcacgtaaa taaggtcata aatcgtacaa aatcgtacaa aacaaaagat atttcaagat
      601 aaatatatta gtgatttcat tgtatcactg aacaatgtaa atattggtaa ggaatttgct
      661 ataaagtttc agtaaataag atgatttgag ttttatatag cgaacaatcc gaaattttgt
      721 ttacgccttc aataaaatat tctttgatga aaattaattg ttactatttt ttaggtttaa
      781 aatcaacaga atatcaatga attttataat acaattatat ttgtaattaa atcttagcaa
      841 agagaaaata aaacccccaa ataaacttta ttaatatgag aaaagtatat gctctaattt
      901 taactttact ggtagtgcaa gctgtaccag cgcaaagttt tagttgggtt aaacatatta
      961 accaaactcc aaatcctaac cttcccaacg agttagatat ttatgatgta aaatactcag
     1021 caataggtaa catttttatt acaggatctt ataaaagcaa ttttcaaata gacggaggag
     1081 taaactatcc aggctttcca gatagttcat caacaaaagc tgatatattt ttaattaaac
     1141 ttgatagtaa tggtgacata aaatgggtaa aaactatggg aagccctgaa ggagatttac
     1201 cagaggctat cgaaatagat aacaatgaaa atatttattt aaccggaagg tttcagggtg
     1261 tctgtgattt tggtagtggt ctgtacttaa cttcttcggg tggatttgat agtttcattt
     1321 caaaatataa ttcatctgga gaaattcaat gggttactaa tactgcctat gggactgcag
     1381 aagaccgaac taatagcata acccttggtt ttgatggcaa cttatacact acaggattat
     1441 ctaaaagttc tacattttat gttggcgatg gaacaattca ggatatatac accaatcccg
     1501 atgcaaaaac cgatctcttc ctggccagct acaccaccag tggcacctac cgctggtcga
     1561 aacaaattcc aggaaacaat aacagctcca tttttagggc cattgcctgc gatgagcaga
     1621 acaacctgta tgtgggggga gcgcttgctg gcacccttac cattgacggg attgattaca
     1681 cctccgctgg aaatggagac attatcctga tgaaggcaca cccaaccgat ggctctgcca
     1741 tttgggtccg taagggtggc agcacggccg acgaccagct gaacagcatc gccgttaacg
     1801 ataactttgc ctacctcatt ggctatatcc aaggaaccgg aaccattgac agcacagcta
     1861 ccctgcaatc ttcacagttc aaaacaaccg gtggaaatga tatctttctg gccaagtata
     1921 accttgaagg acgacttctt tggaagaaaa cattgggtag caccggagca gacgtaggtt
     1981 acggcctaaa cactattaat aatattctgg tggcaaccgg ctacttctcc aacaccctaa
     2041 actttaacct gagctccatc acctccggag gaggcatgga cgccgctttc tttgtatttg
     2101 acgtggagtc gaatgccgtg ctggctaaat ccgtttccgg aacgctggag gatcgagggc
     2161 aaggtgtggc gctggatagc agctacaacg cctaccttgg aggttacttc atgtcttcta
     2221 ccctttcagt aggaactacc actctcacca actccgcttc aacttttaag gatctgtttc
     2281 tggcaaagta ccataacgag tttgccgcgg cattcaccaa caagaaaaac gtaacctgca
     2341 acggcggtag caatggcgag ctgaccgtta ccccatattt tggcacccca ccttacactt
     2401 ttacctggaa gttaaacgga aacccctacg ccgaaacaga ttcagccata acaaacctgg
     2461 gagttggcac ctatgaggtt actgtaaccg actacctata aaacacctct accctttcgt
     2521 acaccgttac ccagcccgct gctctttcca tcggtcacac ccaaaacaac gtgagctgct
     2581 acgggaagca ggacggaaca ataaatatta ctgttagtgg aggtactcct tcatatacat
     2641 acttctggac atcgtccgat ggactagggc atgtggttga tgcggaggac caaacggggc
     2701 ttactgcagg tacctacagc gtggaggtta ccgaccataa tggctgcatg gctaatggca
     2761 gctacaccat aactcaacca accagaatta acatcacagg agaagtgacc aatattaccg
     2821 gcagcggcag caacggcgcg gtggacataa cggtatcggg tggctctgga acatattcct
     2881 cctacgcttg gtacttcaac gatataccgg gcggcacaca ccaggataca acgggtatta
     2941 acaatggagg ggactacaag gtggttgtaa ccgacaacac tgcctgcaag gactccaaca
     3001 cctttactgt tctggacgaa agaatattcc acggatggat tgtcaccaaa acaaatgtta
     3061 gctgtaaggg gggcagcgat ggctctgcta ccgtttcatg ggcagaaaac accggaacag
     3121 tcaccatcct ctggagcccg gacggtgaaa ccaccgcaac tataagcgga aaaccagcag
     3181 gcagctacac ggcaaccctc accgacgaca tgggaactcc cggtgacaca ggggacgata
     3241 tgaccataaa cgttgacgtt gatctcaccg aaccagccca gcctcttggg ggcggtatag
     3301 tgagctaccc ggtaacctgc catggaggaa gcaacggaat tctcgacctc acgccaagtg
     3361 gcggaactct gccctactca tactcttgga ataccatacc ggctaaaaca actcaggata
     3421 tcaacaacct gccggcaggc aattacgagg ttaccataac cgactctaaa ggttgcacta
     3481 cagtagttcc gggaaccgtt agcgaaccag atgcaatttc gttttcattt gctttcaccc
     3541 aacccacctg tcatggtaca atagatggct cactagccgt cagtggccta accggaggaa
     3601 ctccaggtta cagctacctg tggtccaaca cgcttacatc ctcttcgatt agcaatatca
     3661 aatcgggcag ctactggcta aaggttaccg atagcaaggg atgctacaat acccaaagca
     3721 cctatctcga tcaaccggca gcgctaacga ttagccatgt tgccaatcag ccaacctgcc
     3781 cggaatctaa aaacggtagc attggcatca ctgttggtgg tggtacgtct ccgtacacct
     3841 acttctggac aggtcctgac gtggctaacc ccacagacga ggatctggtt aacctgggtg
     3901 taggcacata caatgttacc gttaccgatg caaatggctg caccaaggac taccaggttg
     3961 tgctaaccga agctaaccca tcaccaagcg ctatactctc cagttccgat acgaataacg
     4021 aaatttgcga tggcgacaat gtggtattta ctgcctccgg tggcagcacc tatgaattct
     4081 tcctcaacgg catatcggtt caaggccctg gtgctggcta cgcatacaac aattcaaccc
     4141 taattaacaa cgatgaggta ttcgccgagg ttacatcgtc tgctggttgc aaggccctaa
     4201 ccaacacgat aagcaccatc gttcatacgc ttcccactgt tacggcaaat gccagcccaa
     4261 atcccatttg tgagggcgat ccggttaccc ttaccggaag cggggcaaca tcgtacctct
     4321 gggataaggg ggtaaccgac ggagttccat tctacccaac cacaaccgaa atctatacgg
     4381 ttacaggaac cgatgacaat ggttgtacgg ataccgacca aataaccgtt actgttaacc
     4441 ccgttcccga tgcccagatt aacaccacgg accccacctc atactgcgaa ggtgaaacca
     4501 tcagtacact tctgaatgca tcaccttccg acggttcata ctaccagtgg atacggaatg
     4561 gtgctaacat ttccggtgca tattctaaca catacaccgc ttccgaagca ggcttctact
     4621 cattctatgt catcaaaaat gggtgcatgg ggatcagcga cgaggttgag attattgttc
     4681 acccccttcc ttcagtttcg ctggccgact ttgaacccgt ttgcgccgat gccgagccct
     4741 tcgccatcac gggcggaaat ccggagggag ggatttactc cggaacaggc gttaccggtg
     4801 gaacctttga ccccgcagca gctggtgccg gaacgcattc catcacctac ttctacaccg
     4861 atgccaacgg ttgctctgca agtgcttccg cggatatggt ggtgaacggt ctgcccgtgg
     4921 tttcgctagc tgattttgac ccggtttgcc tcgatgccga gccctttgcc ctttccggcg
     4981 gaacgccgga gggcgggata tactccggaa cgggcgttac cggaggaacc tttgacccnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa cgggcgttac cggtggaacc
     5161 tttgaccccg cagcagctgg tgccggaacg catactatta cctactccta caccgatgcc
     5221 aatggatgct ctgcaagtgc ttccgaggat atggtggtga acggcctacc aactgccaac
     5281 attaacacaa cagacccaac cgattggtgc gagggtactc ctataaatgt aaatttaacg
     5341 gccgatgttg ccgaatccta ccagtggttg ctagatgggc aacccattac gggcgaaacc
     5401 gggcaaacgc tgattgccac acaggcaggg acttacagcg tagctgctac tactaatggt
     5461 tgtaactcaa ctggtaacac aattaccata accgttatat caacaccctc tgtggccatt
     5521 actactgatg atccgacaac atggtgtgcc ggtgatgagg tttcggttca gcttacagca
     5581 ataccctcgg gtggcgatag ctaccggtgg attaaggacg aggtaaccat tgctggtgaa
     5641 acacagtcca actacacggc aaatgagcca ggcaactata gggttgaagc cttatttgcc
     5701 gggggatgca gctccgtttc ggaaattttc tccctaaccg agaaccccgt tccaacggtt
     5761 agcatagcta ccgataccat tcagattgac acctcatcaa cctacacatt tgatgcgggg
     5821 gcaggattcg tatcatacct ttggagcgat acctctaccg gacaaaccct tctggtagat
     5881 ggagccgtta ccggaacagg tgagttcacc tactgggtaa ccgtaaccaa cgagtttagc
     5941 tgttctgctt ccgacacggc agtcgtggtt gtttcacctg tgggtggcgt tcctatgcaa
     6001 atttcgtggg aaatttcaat ctaccccaac ccgtcatcgg gtgagttcaa gctgaaggtt
     6061 tcaggcattg aacccggaac atacaccctt acaattttaa atgccggtgg tcaaattgtc
     6121 tatggatcaa cctttagcgc aagtgataca gaaatcacac gggcaattaa cattcaaacc
     6181 ctatctaaag gattgtacta cgtaagattt gaaaacacga ataagggatt tacccgaaaa
     6241 ataattctga aataatagct ttacttgaat ttatagataa aaggagggtt catttaccct
     6301 ccttttacat ttaaccttga tcttgctggc tattgattga agaaaatagc tcctcgtagc
     6361 tatcggccac agcttcccag ctgtataggt ttttaatcct agtataattc gactgcttag
     6421 cctttaaaac agacatggca ggaagacggc caaactccct aatcctgtca ttcaactccg
     6481 tggtgctgcc gaaataccac ccttcatcac caagaacctc ccggttaaaa gggttgtcat
     6541 gcgctataac aaaacattta caggccattg cctccagcaa cgaggggtta gtgcctccaa
     6601 ccgtatgccc atgtatatag gccaatgaat ggtaacgcaa ggcattcaaa tcatcctggt
     6661 taaaatttgg acctaaaaaa cgaatattct tgaaatgttc aaattttcta tataaacttt
     6721 caccaaaacg agtgtcgtac ctgccaataa taatcagatt ttcgcctaga gcaacaaaag
     6781 agcgagcaat catctcgatg ttgttttcgg gggtcagcct tgaaaccata aggtagtagg
     6841 gttcaaagat tccgtacatt gccaatacat cccgtggggg aatctctcca agcggaaatg
     6901 caccgtaggg tatgacttta ggcttaacgc cataggaagc ggcgtagtag gacaccaggg
     6961 cttggttatc acaaaccaac cgggctacaa agtgcactgc catatactct gaaaacctaa
     7021 ggaatagctt tgcgggtttg ctccatttag accttttatg ctcaagccca tcgagattta
     7081 caacaatctt ttgtctaaac gtttttttaa aaaggggtag ccagggagca aatgaatggc
     7141 cacattccag aataacatca atttcttcag tatttgccca gcatagcgat tttaggtcgt
     7201 aatgaagggt ttggaagttg tggggaagat gtttccctac acctacatta accaggaaca
     7261 acatggagct tagcctggta acgcttctac cttttccatt aaaagttgga gaaagaacgt
     7321 agacaaggtg tccacgagct accagaattt gagacagcac ctctgcaaaa cgttcaaaac
     7381 cgccatagct gttaggtatt cccctagttc caagtattgc aattttcaca agaaagtgga
     7441 ttacggataa attaaaatcc tatactgccg ccattattct tcctgccaac catattttaa
     7501 gaagagaaag agacccctgt aactataagc tgtcaacaag catcaactaa aacaaatgat
     7561 acaattgttt tagctaacct taaaaccatt tcacaagcca aatcaattgc attgtgcggg
     7621 taatctatct tatagctaaa ggattggggt agcaaaaact tttctatgat ttgttttcaa
     7681 accgcgggac tgtgaagaag aatgtagatc ccttcccttc ggtgctctca acagcgatgg
     7741 ttccacgatt tttttcaaca aattccttac ataaaatgag cccaagcccg gttccttcct
     7801 cctcatttgt tcccggagtt gacggatttg aatcaatcct aaaaagcttt tgctgatttt
     7861 cgggagatat tccaacaccg gaatccctaa ccatcacttt aaggtattcg gaatccttga
     7921 caacctcaac ctctattctc cccttagctg gggtaaactt tatggcattg ctgatgaggt
     7981 tgcgtaaaac cgtggtaatc atatccctat cgcaataaac aaaatgatcg tccggattaa
     8041 cccaagctaa ctctatcccc ttctgcagag cgttggcctt aagcagatct atattttcat
     8101 ttatgatttc agccatatta agcgatttca agttgacaac cgttcggcca gtttgaagca
     8161 gcgaccattg aagcaggttt tcgagaagat taaaggtgtt gttggagtac ttatatatct
     8221 gctgaataaa catgtggagc ttttcgggtt caaaactttc aaactccctt gcaagcagct
     8281 cagaaaggct aagcacagca ccaaagggat tttttaaatc atgggcaatt attgcaaaga
     8341 acttgtcttt agtctgatta agtgaaacaa gctcctttcg ctgcacatcc atctcttcct
     8401 tttgcctttt aatttcctga tttttagctg taagctccgc ttcaataaca ttgtaatcgc
     8461 gtaaccgcca caccagggtc ttgagcaacg ctctcgataa aaggggattc ttgtcaagca
     8521 gcaggttgaa ttgcccctgg tccagcctaa gtaattctgt atccctgagt gcggtaatag
     8581 ttgttgacct ggctaaagaa tcgagcatag agtactcacc aaaaaactgt cggggaccaa
     8641 aagtggcaaa tgtatggttg ccgtcatgag ccttcacctg tccactaaca actatataaa
     8701 gggcaaaatc atagtccccc ttgttgaaaa ggacttcgcc cgcttttatg ctttttgaaa
     8761 ctaaactttt ggctattttg cccaacccct cgctatcaac acccatgaat aggggtattg
     8821 atctgagaag cgattcgtaa cctgcatata acgactcgtc ctttccccta ttaaaagctt
     8881 taaacagcat actgaataat ttatgaacaa atataatgta tattatttat acatagaggt
     8941 ttacaactaa tattttttac catacctgat aaatactggc tcttaaaacc attcataatg
     9001 aatttaccat caaatatcat caataaatat ctaaagagta ttacgtaatt ataacctaat
     9061 atgttgaatt ttattaatat tgtgattgtt tttattgctt ttgaaaaggg gaattgtttt
     9121 gaccggcatc acatgaaaac ctatagaaac agcacggttg ttattatgtt aacttaaaag
     9181 aggaacctga aacctggacc aaaatgtgaa aaaatacttg ggcatacatg ctgcactcga
     9241 ttatggtaga gagctaaaaa caatgtttgc ctatatgtaa tttatttaac cttataatcc
     9301 ttctaagcag gtggtatgac tattagttta caatgttgta ggtagaaaaa ttatatttac
     9361 gaccaaacca gggccgatgg ttgcagcatg ttgccattaa taatattgac atattgctat
     9421 ataacaagaa aataaataga agcagatgaa acttaagcta aacattcgcc aaaaaatact
     9481 ggtttacatt ctaggtccat cgatagtagt atttgctggc atattcacat atactacccg
     9541 gacctcaagg gatattgctt acaaccaggc aatcaagcta accgatagct atgcgcatca
     9601 gtatgccttg aacattgaag gatggcttaa ccaggattta gcgattaccc gtacactggc
     9661 cgcagctttt ctggaataca agcagatgcc gtttgagcaa tggcaacggt tgatataccc
     9721 aatgtacgac agaataatcc taaccactcc gcatattgac gcctactggg atagctggga
     9781 aatgagcaat cttgacccca catgggacaa accctacggc cggtactttt acattgttta
     9841 caaggaaaac ggggtttaca aaaccaaagc agaacttaga agtcttacag gcgaccctga
     9901 tacctatgga gccatgaaga aagcggcaaa agaaattatc aacgaaccct atgtctcggc
     9961 tctacaaaga ggccagctga tgacaaccat ttcgtcgcca cttgttgaaa atggaaaatt
    10021 tatcggacta atcggtattg acttgctgct caccagattt caaaatttgg ttaacggcat
    10081 taaaccctac cccaacagct atgccttcct gctttcaaac aaaggaaact ttatagccca
    10141 ccccgattca acaatttatc aaaaaaatat tgccgaacgc cttccggaac tgaatagtag
    10201 attcagcatt ctttccaacg ttcagaaagg aaaggcgttt agcttcaccc aaaccggtga
    10261 aaatggccaa cgtctttact acacattctc gcctatcaac attggcaata cacaaactcc
    10321 atggtctgtt ggcatagcgg tacctatcaa cgatattctt tacgaagcca acaaaaacta
    10381 caacattagt attgccatcc tgattttagg cttgattctt cttatagcaa ttatattttt
    10441 cgtttcgagc agtattacca gaccaattaa gagcattacc catctgctcc aggatttagc
    10501 taacggtaag gttgatagca ccctggcaat caagcacgac gtccatgacg aaattgggaa
    10561 aatggcgcta gcgttaagcg agagcattaa gggaataaat gacaaaacag aatttgcaag
    10621 agaaatcggt tcgggtaacc tcaaggcaga ggtttcgctt ctaagcgagg atgaccacct
    10681 tggaaaatcc cttttagaga tgcgtgataa cctgcaaaaa gcaaaggagg acgaagaaaa
    10741 acgcaaggct gaagacgaga aaaagaggtg gaccaacgag gggttggcaa aatttggcga
    10801 tatactccgc caaaacaaca acaacctatc caaactaagc gacgaaatta tcaaaaacct
    10861 tgtttggtac ctcaacgctt cgctgggcgg aatatttgtt ctcaacgaga atagggaaac
    10921 cggggataaa acattcgatc tggttgctgc ttttgcatac gaccgcaagc gcattcttaa
    10981 caagtcttac cagtttgccg aagggctagt tggggcctgc gctgccgaaa aggacatcat
    11041 catgttaacc gaaatacccc aggagtatat cgaaataacc tccggacttg gcgggactaa
    11101 cccaaacttc ctgctcctta ctcctttggt taacgaggat gaggttatgg gagttattga
    11161 aattgcctcg cttagaaggt ttgaagagca tgaaattctt tttgtgaaag aaatagcgaa
    11221 gagtattgct accacactac actcggttaa ggtaaactcg ctaacagccg aactccttat
    11281 gaaatccaag gaacaggctg aaatcatggc agctcaggaa gaggagatgc gacagaacat
    11341 ggaggaacta caggccaccc aggaggaagc tgccagaaaa acttttgagc ttgagggttt
    11401 ggccaacgcc ctaagcgctt catccctgat aatggagtac gaccctgcag gagtaatcac
    11461 cagcattaac gaaaagtact ccgagctact aagcatttcc agggaggata taatcaacaa
    11521 acattactcc gacatagcta ccgaggcaat agataaggaa aagctggata aactctggca
    11581 ggagattcgc tccggaaaaa ccaaatatag aaaagtcgta ctcaatataa aaggtaaaga
    11641 gcataccctg gtcgaaacct acacgccaat cctcgacacg cagggtcggc tttacaaggt
    11701 gttaaaaata gcaaccgata taaccaacct atagaaaata aagggctgtt gaaaatgcag
    11761 ccctttccat tttaattcta tgctgattta gccccagcgc agaaaatgct gctgtcaata
    11821 aatttggcat tcaatatcat aaacgatttt gcataacata gctccctcct tgctatagcc
    11881 atcttggcat cctcattctg gcagaagatg acgactcgat agaatgtact tcacttttct
    11941 accaaaaatt attttttgga ccgccttcgg gtagtttaga aataacaggc aatttccaca
    12001 attaggtaac tctggtttct cttatcataa aatatttaaa aaataattta tctatgtatt
    12061 gcatagtagt aattttatta ctatatttgc atcgtattaa taccataatt tacaaggtat
    12121 aaattttgcc atcgtatgaa aaaaactcta acagtaagcc taaacggcag gtcgtacgag
    12181 ctggaggaag atgccttcaa aatgctcgat gagtacctta agcagctgga atcctacttt
    12241 gccgaggatg ccgggaaaca ggaaatatta agcgacatag aatcgcgaat tgctgaccac
    12301 tttgacgaat ccagcagggt tcggggcgtg gttatagggg cagatgaggt gaggcgggta
    12361 attgaaatta tgggaacgcc tagcgatatt gccgatggag aaaaagtgcc gaaagcagaa
    12421 aagccccata ggagctaccg taagctctac cgcaacatgg acgataaggt tctgggcggt
    12481 gtgtgctcgg gcatgggatg ctactggaat gtggagcccc ttctcttccg aatcatcttc
    12541 atcatttcaa tcttctgggg tgggcttggt cttttggtgt acgcaatttt atggatagct
    12601 gtacctccgg ctctaaccac aacccaaaag cttgaaatgc gtggtgagga ggttaccgcc
    12661 gagaatattg gtaaatcgtt tgaaaacaaa aaataaccct acggatatgg aagtcgaaag
    12721 caccgagagt atgcatagct ccagtattcc tttgagcatc tg
//
LOCUS       DAOPHX010000071        12733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_5816_length_12733_cov_5.963480, whole genome shotgun sequence.
ACCESSION   DAOPHX010000071 DAOPHX010000000
VERSION     DAOPHX010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5816_length_12733_cov_5.963480"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(329..2440)
                     /locus_tag="PK502_06565"
     CDS             complement(329..2440)
                     /locus_tag="PK502_06565"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPH00514.1"
                     /translation="MKYSKTILYLCSMTLLISNSGCNKANKPAAPEPLSINVSLSDSE
                     KAAGLLTPEILWKFGRVGEYALSPDGTMLVYAVTFYNLQENKGVTNLYVVSATGGEPL
                     KLTDETGSESAPQWSSDGQTILFLSTRGGSNQLWQVRPDGSELKQISGIDGDINGFSF
                     SPDNTKLVFAVDVKLDQQADDIYPDMPKAKVRIIDNLMYRHWNYWEDGRYSHLFVADF
                     ADGKISNARDINAGEKWDTPMAVDFDMTEVQWSPDGSKLVYASKKLYGREYAVSTNSD
                     IYLYDMVSGATTNLSADNPGYDRYPSFSPNGAYISWWSMKTPGYEADQKKLVLFDTKS
                     NAKTTLTESFDQNVDSYVWDGDSRNIYFASEVKGTAQLYKLNISTDSIYQLTRGAHNY
                     TSCQKGNGVLVAERMSMSMAPELYRIDEQNGSAQQLTFTNKQIYESIKMGEVRERWVK
                     TTDGKEMLVWVILPPNFDETKKYPALLFCQGGPQSTVGQFWSYRWNFQIMAANGYVVV
                     APNRRGVPSFGQEWTRQISGDYSGQNIDDYLSAIDDVKKEPWVDANRLGCVGASYGGY
                     SVFYLASHHQKRFKAFIAHCGMYNLESFYAQTEETFFAHFDLGGAPWEGNNPVARRSY
                     ANSPHKFVQNWDTPIMIITGEHDYRIPYTQSIEAFNAAQLRGIPSKLLFFPEETHFVT
                     KPQNSVIWQREFFAWLDRWLK"
     gene            2678..4300
                     /locus_tag="PK502_06570"
     CDS             2678..4300
                     /locus_tag="PK502_06570"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPH00515.1"
                     /translation="MNRAIPFLALLFFSCQDGNIKRAINSITSQEMGQDIMALSSDSL
                     LGRAPFTVGEERTVAYLQKRMAELKLTPAFEGSYIQSVPMVSITTMVPNTVDFTTPKG
                     ALKLKTDEEICLWSPTIKPNIKISSADLVFAGFGIDAPEYEWNDFAGLNVSGKIIVVL
                     VNDPGFYTHDSLLFTGNAMTYYGRWRYKFEEAERKGALGCLIVHEDAAAGYPWAVAGS
                     KSNTPILYMDSPELNNSRCVLNGWITLDAVRKLMGKCGYSYDSLKQLAVNRGFKPVGL
                     KACLSLTMENRQTKSASKNVAGYIAGSKNPDEFIVYTAHWDHLGIGKAVDGDSIYNGA
                     SDNAAGIAWMLSIAKAFQNSGERPERSVLFLSPTAEESGLFGSTYFVQHPPQEVGRMV
                     ACFNYDVALFLGKFSDVTITGPGHSNLDSLLRLAAKSQNRYVISDPNPEIGMIYRSDQ
                     LPFLKAGIPSMFAKGCIHQTELGKEKTQELINKYWLNTYHKPSDEFDPTRDNLDGLVE
                     DARLFYDMGNRLTSWNQYPKMSRTSEFYKRSR"
     gene            4576..6528
                     /locus_tag="PK502_06575"
     CDS             4576..6528
                     /locus_tag="PK502_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00516.1"
                     /translation="MKLSTLIKNTEDFFSERRLLYIILTGILIIDLTILVFSSGYYGG
                     ADTAGHYLSSHWAFAHPAYLLNHWSKPIFTLLSAPFAAVGFKTFQLFNILFGIATGYL
                     SYLVAKELKMKSPLLAIVICCFTPIFMISLFSGLTEILFAFVAILASYLLIKERYAWG
                     AVVVSFLPLIRYEAVVLLPVYAIFLINKKHYREILLMLTGMVIYSLLGALAGKNILWL
                     FTEFPHQNTVGVYGKGKFFHYILRSPGFFGIPNEIFFVTGLVAGLSLYLREKMEYSKE
                     FLLVVLPFLTYFFAHTVSWWLGIGGSQGQNRYMAAIVPFMAVMATRGLYLFAKMFLIL
                     TKQEWVRVFALIIGFISVFHIPFAVQNYPITPGPTEKTIRQATDWIKQEGLNNVKFHY
                     NDPTVVLQLNATPFDTTECERFVATPANPAKGLENGELLIYDTYFTEMAGGNLNELAS
                     CSDLELLKVFEPEVPVDVYRKPYKIAVFKKIEPNPFVIQKNSSLLKGEGIIFCTIKNF
                     DFDSTTLQTEAKHIKPMGKNGSSCILLKESRDFCLKQNFELDSLKFVHPFELQVSMSI
                     LPTEPEKKLRYVVEATSNNKQIAYKSYDITFTEQNTSDEWVNREFRIAVPEIANQKNV
                     RIKTYIWNNRKGRYLIDDYTISYRIQ"
     gene            6743..7726
                     /gene="fmt"
                     /locus_tag="PK502_06580"
     CDS             6743..7726
                     /gene="fmt"
                     /locus_tag="PK502_06580"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPH00517.1"
                     /translation="MSITPRIVYMGTPDFAVEPLRAIKDNGYNVVAVVTAPDRPAGRG
                     LKTQPTAVKTFALEHSLPVLQPERLKDEHFTRQLEYLKPEIGVVVAFRMLPKSVWSIP
                     RLGTFNLHASLLPQYRGAAPINWAIINGEKTTGVTTFLLDEQLDTGNILLQKEVCIGE
                     DETAGELHDRLMMAGADLVVKTLDMLLSGHQVNTIPQHQLMVAQPPLRTAPKLFRETC
                     RIRWNDSVSNVYNLIRGLSPYPTAWSEMLVSINGKELLLQVKIYSAAKSVEPTSNLQP
                     GSIDTSSSRTLRVACSDGFIALKIIQQAGKKAMPVEEFVKGWHNTKFIKML"
     gene            complement(7721..8683)
                     /locus_tag="PK502_06585"
     CDS             complement(7721..8683)
                     /locus_tag="PK502_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HPH00518.1"
                     /translation="MKFWLVTVKKIAFFFAFTMLLHASAWAQSSMSGSDYIDRYSGIA
                     VRNMKQYGVPASIILAQGMLESNNGNSTLAREANNHFGIKCHKDWTGPTIFHDDDHKN
                     ECFRKYRTPENSFVDHSLFLRGAARYAFLFELEPTDYRGWAYGLKKAGYATNPSYPEL
                     LIKIIEENKLYLYDKGVEVAVASPANGRGKSVDVDSYEIDIYGGRTVYTRNRIKYIVV
                     KEGDSFESLTKELNLMPWQLFKYNDLTSDSTLRVGEELYIQPKRRKAERNHSVHTVEK
                     GETIYQISQMYGVKLNVLYRRNNMHPGDSTENGQVVFLRGRKPL"
     gene            complement(8888..9580)
                     /locus_tag="PK502_06590"
     CDS             complement(8888..9580)
                     /locus_tag="PK502_06590"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPH00519.1"
                     /translation="MDFTPDRILFEDNHYIAVNKLCGDIVQADKTGDLSLLETVKQFI
                     KVRDGKPGNVFLEVTHRIDRPVSGVIVFAKTSKGLSRMNHLFQESQVQKRYWAVIRSK
                     PPKDTDLLEHYLVRDSKSNKSFVYNHEVNGSKVARLRYNLVGKSEKYYFLEIELITGR
                     HHQIRCQLAKIGCPIKGDLKYGFPRSNPDGGIHLHSRLFSFTHPISGEQVVITAPPPK
                     DVLWDEFMLLGI"
     gene            complement(9580..10395)
                     /gene="panB"
                     /locus_tag="PK502_06595"
     CDS             complement(9580..10395)
                     /gene="panB"
                     /locus_tag="PK502_06595"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPH00520.1"
                     /translation="MSTESTAKVVTTHVLNEMKLRGEKIAMLTAYDYSMARILDAAGI
                     DVLLVGDSASNVMAGYQSTLPITLDQMIYHASSVVRGVKRALVVVDLPFGTYQGNSKL
                     ALNSSIRIMKESGADAVKLEGGKEILESVDRILSAGIPVMGHLGLTPQSIHKFGTYVV
                     RAREEEEASKLVDDAHRLQDAGCFSIVLEKIPASLGARVAHELKIPIIGIGAGNGVDG
                     QVLVTHDMLGITQEFSPRFLRRYHNLFAEMLGSFQAYIKDVKSLDFPNEREQY"
     gene            10815..>12733
                     /gene="dnaK"
                     /locus_tag="PK502_06600"
     CDS             10815..>12733
                     /gene="dnaK"
                     /locus_tag="PK502_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPH00521.1"
                     /translation="MSKIIGIDLGTTNSCVAVMEGNEPVVITNSEGKRTTPSVVGFVE
                     NGERKVGDPAKRQAIINPQKTVFSIKRFMGETYDKVANVEVPRVPYKVVRGENNTPRV
                     DIDGRLYTPQEISAMILQKMKKTAEDYLGQEVTEAVITVPAYFSDSQRQATKEAGEIA
                     GLKVRRIINEPTAAALAYGLDKKNKDLKVAVFDLGGGTFDISILELGDGVFEVKSTNG
                     DTHLGGDDFDHVIIDWLAEEFLKDEGIDLRKDPMALQRLKEAAEKAKIELSSTTSTEI
                     NLPYIMPVNGIPKHLVKTLTRAQFEKLTDHLFQKTIEPCRKALADAGLKASEIDEVIL
                     VGGSTRIPYVQQLVENFFGRTPNKSVNPDEVVAVGAAIQGGVLTGEVKDVLLLDVTPL
                     SLGIETLGGVFTKLIEANTTIPTKKSEVFTTAADNQPSVEIHILQGERPLAKDNKTIG
                     RFHLDGLPPAPRGIPQIEVTFDIDANGILHVTAKDKGTGKEQKIRIEASSGLSDDEIK
                     RMREEAKANEAADREAKERIEKLNNADSLIFQTEKQLKEFGDKLPASKKEPIERALSK
                     LKDAHKNQDVSAVEVASNELNAAWQAASEEMYKASQETAGQQANPGAGTSSSSSSSST
                     GSNDAEVTDVDFEEV"
BASE COUNT         3613 a         3022 c         2830 g         3268 t
ORIGIN      
        1 caactcgccc cgcctacggc gtggctcaaa cagtgccgct tgggcattcc cgcgccacgt
       61 tccgcccccg acccatttgc gggtctggct tttatgcctt ttttctaata ccagcaacgt
      121 tcaacgtttc ccgcctttac gagctccgcg gagaataact ttccccagtt ggggctgaag
      181 acgagggtaa aggatagagg agaaaggaaa cttgtccgcc taccggcgga ataatcattt
      241 cctgaaatgt atgccttatg caaaacggca agatggcgct atacgcacca tcttgccgta
      301 agaatcatat ttagtggagg aaaagttttt acttcagcca cctgtcgagc catgcaaaaa
      361 attcccgttg ccaaatcacc gaattctgtg gtttagttac gaagtgggtt tcctcgggga
      421 agaaaagcag cttgcttgga attccgcgaa gctgtgcagc gttaaatgcc tcaatgctct
      481 gggtgtaagg tattcggtaa tcgtgctcac cggttatgat cataatcgga gtatcccagt
      541 tctgaacaaa tttatggggt gagtttgcat agcttcggcg ggcaacgggg ttgttccctt
      601 cccatggagc acccccaagg tcgaagtggg caaaaaacgt ttcctcggtt tgagcgtaaa
      661 acgactcgag gttatacata ccacaatgtg caataaaggc cttaaaacgt ttttggtgat
      721 ggctagccag gtagaatacc gagtaaccac cgtagcttgc gcctacacac cccaaccggt
      781 tggcatcaac ccagggttcc ttcttcacat catcaatagc gctaaggtag tcgtcaatat
      841 tctgtccgct gtagtcgcct gaaatctggc gggtccattc ctgaccaaac gatggaactc
      901 ccctacggtt gggtgcaacc accacataac cgttggcagc cataatttga aagttccacc
      961 ggtagctcca gaattgccca acagtgcttt gggggcctcc ctggcagaaa agcaaagcgg
     1021 ggtacttttt agtttcgtca aaattgggtg gaagtatcac ccaaaccagc atctccttac
     1081 catcggttgt ttttacccaa cgctcgcgaa cctctcccat ttttatgctc tcataaattt
     1141 gcttgttggt aaaggttagc tgctgagcgc taccgttttg ctcatcaatc ctatatagtt
     1201 cgggagccat gctcatactc atccgctcag ccaccagcac gccgttgcct ttctggcagg
     1261 atgtgtagtt gtgagcaccc ctcgttagct ggtagatgct gtccgtggaa atgttaagct
     1321 tatagagttg agcagtaccc ttcacctcgc tggcaaagta gatgtttcta ctgtcgccat
     1381 cccaaacata gctatcaacg ttttggtcga agctctcggt aagggtggtt ttagcgttac
     1441 ttttagtgtc gaataacacc agctttttct gatcggcctc ataacccgga gttttcatgc
     1501 tccaccagct gatgtatgct ccgtttggag aaaagctggg ataacggtca taccctggat
     1561 tatcagcgct aaggttagta gtagcgcctg aaaccatatc gtagagatag atatcggagt
     1621 tggtgcttac agcatattcc ctaccgtaaa gtttcttgga ggcgtaaacc agcttactac
     1681 catcggggct ccactgtact tcggtcatgt caaaatcaac agccataggg gtgtcccact
     1741 tttctccagc atttatatcg cgtgcattcg agattttgcc atcggcaaag tcagcaacaa
     1801 aaaggtggct ataccttccg tcctcccagt aattccaatg gcggtacatc agattgtcaa
     1861 taatcctcac cttagccttc ggcatatcgg ggtaaatatc atcggcctgt tggtcgagct
     1921 taacatcgac ggcaaacacc agctttgtgt tatcgggcga aaaggaaaaa ccatttatat
     1981 cgccatcaat gccagaaatc tgtttcaact ccgaaccatc aggcctcacc tgccatagct
     2041 ggttcgaccc accacgggtt gaaaggaata ggattgtttg gccatcgctg ctccactgtg
     2101 gtgcagactc agaaccggtt tcatcggtta actttagagg ctcaccaccg gtagcagaaa
     2161 caacatacaa gttggtaact cccttgtttt cctgtagatt gtaaaaggta accgcgtaaa
     2221 caagcattgt accgtccgga gaaagggcat actcgcccac ccgcccgaac ttccatagta
     2281 tttcgggagt tagcaatccg gctgcttttt cagagtcgga aagagaaaca ttgatgctca
     2341 acggttccgg agcagctggt ttgttggcct tattgcatcc ggaattggat attaacagcg
     2401 tcatggaaca gagataaaga attgtttttg aatacttcat ggtaaagtgc gttagagttg
     2461 aactttggtt tggactgcta aaattagcaa aagttcacct acagctaaaa ggtgttgtta
     2521 gaaaaaaata ggcacatatt caaacaacct acaaaccaca aggggttaac ccaccagtta
     2581 agttaaacca aaatctactt gtggtatcga aatatccgct acagataaat gctgataaat
     2641 tgattacatt tgctttcgac catataaact taatatcatg aatagagcga ttccgttttt
     2701 agccctactg tttttctcct gccaagatgg caatattaaa agggcgataa actccattac
     2761 cagccaggag atgggccagg acataatggc actatcctcc gatagccttc tgggaagggc
     2821 accattcaca gtcggggaag agcgaacggt ggcctacctg caaaaaagga tggcagagct
     2881 aaagctgacg ccagcattcg agggctcata tattcaaagc gttcctatgg tttcaataac
     2941 caccatggta cccaacactg ttgactttac cactccaaaa ggagcgctga aacttaaaac
     3001 cgatgaggaa atatgtctgt ggagcccaac cataaagccc aatataaaaa taagcagtgc
     3061 cgatttagtt tttgccggct ttggtattga tgcccctgag tatgagtgga acgactttgc
     3121 aggattgaat gtgagcggaa aaataatagt tgtgctggta aacgacccgg gattttacac
     3181 ccacgattcg ctactgttta caggcaacgc catgacctac tacggtcgtt ggcgctataa
     3241 atttgaggaa gccgaaagaa agggagccct aggctgtttg attgttcacg aggatgcggc
     3301 tgcaggctat ccatgggctg ttgccggaag caagagcaac actccaattc tctacatgga
     3361 ttcacccgaa cttaataact ctaggtgtgt gttgaacgga tggattaccc tggatgcagt
     3421 caggaagcta atggggaagt gcggctacag ctacgactcg ctcaagcagc tggcagtaaa
     3481 caggggattc aagcctgtag gcctaaaagc ttgtttaagc ttaaccatgg aaaacaggca
     3541 aacaaagtct gcctctaaaa acgtggctgg ctacatagca ggaagcaaaa atccagatga
     3601 atttatagtt tacactgccc actgggacca ccttggaatt ggcaaggctg ttgacggcga
     3661 ttcgatatac aacggcgcat cggacaatgc tgccggcatt gcatggatgc tctccattgc
     3721 gaaggcgttt cagaattcag gtgaaaggcc cgaacggtcg gtgttatttt tgtcaccaac
     3781 cgccgaggag tctgggcttt ttggttcaac ctactttgtt caacacccac cccaagaggt
     3841 tggacgaatg gttgcctgtt tcaactacga cgtagccttg tttctgggaa aattcagtga
     3901 tgttaccatt accggtcccg ggcattccaa tctcgacagc ttgttgaggc tggcggcaaa
     3961 aagccaaaac cgctatgtga tttccgaccc aaatccggaa attgggatga tttaccgatc
     4021 cgaccagcta ccatttctaa aagcaggtat cccatcaatg tttgcgaaag gttgcattca
     4081 tcaaaccgaa cttggaaaag aaaaaacaca ggaacttatc aacaagtact ggcttaacac
     4141 ctaccataaa ccatctgatg agtttgaccc cacaagggat aatctggatg gattggttga
     4201 agatgcaagg ctgttttacg atatgggaaa ccggctaact agctggaacc aataccccaa
     4261 aatgagtcga acatcagaat tctacaagag aagccgctag ccaaagctaa ccttggcaat
     4321 tgccgctcat ctggtaatct taaaaaaatc ggctcaaagc agaagctgga ttatagtcgt
     4381 aaagttgaac catacaggcc ccattgctga aaaaggaata gttttaatct tgtattaata
     4441 aaatggtaac ctgtttgttg gcaaagaaag tgtagattat gctattttta cgggcgcatt
     4501 gagcataact tcaatcttgc tcaactgcta aaatacatag cataaaaacc ggcaatcaca
     4561 atcaaattaa ccgacatgaa actttcaact cttatcaaaa atactgagga tttcttttca
     4621 gagaggcgat tgctctacat aattcttacc ggcattctga taattgactt aaccattctg
     4681 gtttttagta gtgggtatta cggaggtgcc gatactgccg gacactacct ttcttctcac
     4741 tgggcatttg cacatccggc ctacctgctt aaccactggt caaaaccgat ttttacactt
     4801 ctaagcgccc cgtttgcagc agttgggttc aaaacctttc agctgttcaa catccttttt
     4861 ggcattgcca cgggctactt atcgtacctg gtagccaagg agctgaaaat gaaaagtccc
     4921 ctgctggcca ttgtgatatg ctgctttacc ccaattttca tgataagctt gttttcggga
     4981 ttaaccgaaa ttctttttgc ctttgttgct attcttgcct cgtaccttct aataaaagag
     5041 cggtatgcct ggggtgcagt tgtagtttca tttcttccgc tgattagata tgaggcggta
     5101 gttctacttc cggtttacgc cattttcctg ataaataaga agcactaccg tgaaatattg
     5161 ctaatgctta ccggcatggt tatttacagc ctcctgggag cgcttgccgg caaaaacatt
     5221 ttatggcttt tcactgaatt tccacatcag aataccgtag gggtatatgg aaaaggtaaa
     5281 ttctttcatt atattcttag aagtccaggt ttttttggta ttcctaacga gattttcttt
     5341 gtaacggggc tagtggcagg cctttcactt tacctgcgcg agaagatgga gtactccaag
     5401 gaatttctgc tggtagttct tccctttctt acctactttt ttgcccacac ggtatcatgg
     5461 tggcttggaa tcggtggatc tcagggacaa aacaggtata tggctgcaat tgttcccttc
     5521 atggcagtta tggctactcg tggcctctac ctttttgcca aaatgtttct tattctcacc
     5581 aagcaggagt gggtacgggt ttttgcgcta attatcgggt ttatttcggt tttccacata
     5641 ccttttgccg tgcaaaacta cccaattaca cccgggccta ccgagaaaac aattcgccag
     5701 gctaccgact ggataaagca ggaaggactc aacaacgtta aattccatta caacgacccc
     5761 acggtagtac tccagctgaa tgcaaccccg ttcgatacga cagaatgtga aaggtttgtc
     5821 gcaacacctg ccaaccccgc taagggatta gaaaatggcg aactgctaat ttacgacact
     5881 tacttcaccg aaatggctgg aggcaacctc aacgagcttg ccagctgtag cgatctcgag
     5941 ctgttaaaag tttttgaacc cgaagtacct gttgatgtct atcgaaagcc atacaaaatt
     6001 gctgtattca aaaaaatcga acccaacccc tttgttattc aaaaaaacag ctcactatta
     6061 aagggtgagg gaattatatt ctgcaccatt aaaaattttg atttcgactc aactaccctt
     6121 caaaccgaag ccaagcacat taaacctatg ggaaagaacg gctcaagctg tatactactg
     6181 aaggaatcga gggatttttg ccttaagcaa aactttgaac tcgactccct aaagtttgtt
     6241 catccctttg aactgcaggt ttctatgagt attcttccca cggagccaga aaaaaagttg
     6301 aggtatgttg tagaggctac cagcaacaac aagcaaatag cctacaaaag ttacgacata
     6361 acctttaccg aacaaaacac ttctgatgaa tgggttaaca gggaattccg catagccgtt
     6421 cctgaaatag ctaaccagaa aaacgtcagg ataaaaacct acatttggaa taataggaag
     6481 ggaagatacc tgattgacga ctacacaata agctatagga ttcagtagaa aggatttggc
     6541 tatccttaga aaattttcgc tctcatcaaa gtaaaggaac gaattgataa atcctaaaac
     6601 cgattaggtt tggagtaaaa gcaaagtttt tggagtgccc aaaagattat accatggaaa
     6661 atcttgttca tgctgccact tccaaaaaaa tgttaatata agttagcctt tagtaaacag
     6721 tagtagaaaa gcaaattcca aaatgagcat cactccgcga atagtttata tgggcactcc
     6781 cgattttgca gttgaaccgc ttagagccat taaagacaat ggatacaacg tggttgctgt
     6841 tgttaccgca cccgataggc cggcaggtcg tggactcaaa acacaaccta cagcagtaaa
     6901 gacttttgct ctggaacatt cccttcctgt tcttcaaccc gaaagactga aggatgagca
     6961 tttcaccagg cagctggaat atctgaagcc cgaaattggg gtggttgtag cttttcgtat
     7021 gcttccaaaa tcggtttgga gcattcccag gctgggcact tttaacctcc atgcatcgct
     7081 gcttccgcag tacaggggag cagcgccaat caactgggcc ataataaacg gtgaaaaaac
     7141 tacgggcgta accacctttc tgctcgatga gcagcttgat accggcaaca tcctgctgca
     7201 aaaagaggtt tgcattggtg aagatgaaac agccggcgag ctgcacgata ggctgatgat
     7261 ggctggtgcc gaccttgtag taaaaacact cgatatgctt ttgtccggac atcaagttaa
     7321 taccattcca caacatcagc tgatggttgc gcagccccct ctacgaacag cccctaagct
     7381 attcagggaa acctgccgga taagatggaa cgatagtgta agcaatgttt acaacctcat
     7441 tagaggtttg agcccctacc ctaccgcatg gtctgaaatg ctggtaagca taaacgggaa
     7501 agaattgtta ttgcaggtta aaatatacag cgctgctaaa agcgtcgagc caacaagtaa
     7561 cctgcagccc ggatctattg atacaagtag ctcacgtacc ttaagagttg cctgttcaga
     7621 cggtttcata gcgctaaaaa tcatccagca agcagggaaa aaagctatgc cagttgagga
     7681 gtttgtgaaa ggatggcaca acacaaagtt tataaaaatg ctataaaggt tttcgaccac
     7741 gaaggaatac cacctgcccg ttttcggtgc tgtcacccgg atgcatgttg ttgcgtcggt
     7801 agagaacatt aagcttaaca ccatacatct gcgatatctg gtagatggtc tcccctttct
     7861 caaccgtatg aaccgaatga ttcctctcgg ctttacgccg tttaggctga atgtaaagct
     7921 cctctcccac ccgaagggtc gaatcgctag ttaggtcgtt atacttaaaa agctgccagg
     7981 gcatgaggtt aagctccttc gttagcgatt caaaggaatc accctcctta acaacaatgt
     8041 acttaattct attgcgggta taaaccgttc gtccaccgta gatgtcaatc tcatagctgt
     8101 caacatcaac acttttcccc cttccattgg cgggtgaagc tacggcaacc tcaaccccct
     8161 tatcgtagag gtaaagcttg ttctcctcaa ttattttgat tagcaactcc gggtagcttg
     8221 gatttgttgc gtatccggcc tttttgagac cgtaggccca tcccctgtaa tcggtaggtt
     8281 caagctcgaa aagaaaggca tacctagctg ctccccgaag aaaaaggcta tggtcaacaa
     8341 aagagttctc gggagttcgg tactttcgga agcactcatt tttatggtca tcgtcgtgga
     8401 aaatggttgg gccggtccag tccttatggc actttatccc gaagtgattg ttggcctcgc
     8461 gcgccagggt gctgtttcca ttgttgctct ccagcattcc ctgcgcaaga ataatacttg
     8521 ccggaacgcc atactgcttc atgttcctga cagctatacc ggaatacctg tcaatataat
     8581 ctgaacctga cattgatgac tgcgcccatg cactagcatg gagcagcatg gtaaatgcaa
     8641 aaaagaatgc aatttttttt acggttacca accagaattt caaggtaata accaatttaa
     8701 tcccattatg cttcatttaa aattcctaac aaggtatctt atacatgata aattcgtcca
     8761 ataagctggc taccagcacc tgttatcatg ctaatatata tccggctact agtgttgttt
     8821 aagaattttc ctaagttgtg gcagcaaaga taacgaatat aagccattgc tcgaaatcaa
     8881 cattcagcta tatacccagc aacatgaatt cgtcccatag cacatccttt ggaggtggag
     8941 ctgtaataac aacctgctct cccgatatgg gatgggtaaa ggagaaaagc ctggagtgta
     9001 gatgaattcc accatccggg ttggagcggg gaaaaccata cttcaagtcg cccttaatgg
     9061 gacaaccaat tttggcaagc tgacatcgta tttgatggtg cctgccagta ataagttcaa
     9121 tttccagaaa gtagtacttt tcagacttac caaccaggtt gtagcgcaac cgtgccacct
     9181 ttgagccatt cacctcgtgg ttgtatacaa atgatttatt tgacttggaa tcccttacca
     9241 ggtagtgttc cagtaaatcg gtatccttag gaggtttact ccgtataacc gcccagtagc
     9301 gtttttgcac ctgcgattcc tgaaacaggt ggttcatcct gctcagaccc ttgctggttt
     9361 ttgcaaaaac aatgaccccg ctcacaggcc tgtcaatgcg atgggtaacc tcaagaaaga
     9421 cgtttcccgg cttgccatcc ctaaccttaa taaactgctt tactgtttcc aacaacgaaa
     9481 gatcaccggt tttatcagcc tgcacaatat caccgcaaag cttatttacc gcaatgtagt
     9541 ggttatcctc aaagagaatt ctatcgggtg tgaaatccat cagtactgct cgcgttcgtt
     9601 ggggaagtca agcgacttaa cgtccttaat gtatgcttga aatgaaccaa gcatttcggc
     9661 gaagaggtta tggtacctac gtaggaaccg tggcgagaac tcctgggtta tgcccagcat
     9721 atcatgtgtt accagcacct gtccatccac accattcccg gcaccaatac caataatggg
     9781 tattttcaac tcgtgggcaa ctcttgcacc cagcgaggca ggaatctttt ccaatacaat
     9841 tgaaaagcag cctgcatcct gcagcctgtg agcatcgtca accagcttgc tggcctcctc
     9901 ctcctcgcgc gccctaacca cataggttcc aaacttatga atggactgtg gcgttaggcc
     9961 caagtggccc attacaggaa tgccggctga tagaattcta tccaccgatt caagaatttc
    10021 ctttcctccc tccagcttaa ctgcatcggc accactttcc ttcataatcc gtatggatga
    10081 gttcagcgcc agctttgagt tcccctggta ggttccaaat ggcaaatcaa caaccacaag
    10141 ggcacgctta acaccacgaa ccaccgagct ggcatggtaa atcatctggt ctagcgtaat
    10201 tggaagagtt gactggtagc cggccatcac attagaggca gaatcaccta ccagcaaaac
    10261 atctattccg gcagcgtcaa gaatgcgagc catggaataa tcatacgctg taagcatggc
    10321 tatcttctct cccctcagct tcatctcgtt cagcacatgg gttgtaacca ctttggcagt
    10381 actttccgtt gacatattca gctgtattaa ttatttgatt aggcaaaagt aaaggaataa
    10441 tccaacttat tgaaattttg gctagggcag tagctacagg actatgccaa actgacaaaa
    10501 cggcagcctg acaagagtcg ctcatatacc cataaaaaaa acccgggttg ttgttttgac
    10561 cagataatga gcacatctca tgcaattcat caagattgta caataagtta cacctatcct
    10621 ttcctcttct tttcccttca atggcaattc agctataaac aaccgggctg ccaaaaaagc
    10681 ttactgtttg tggcgataaa tgccaatttt gccaaaaaac attgccacaa atacatggca
    10741 taaacattgc agaaacaggt tatgctaaac atattggtag ttgaagtaaa caataaataa
    10801 ataatcttag aacaatgagc aaaataattg gaattgacct gggaacgacc aactcatgcg
    10861 ttgccgttat ggagggcaac gaaccggttg tcataaccaa tagcgaaggt aagagaacaa
    10921 ccccatcggt tgtaggcttt gttgaaaatg gggagcgcaa ggtaggagac cccgcaaaac
    10981 gacaggctat tatcaaccca caaaaaaccg tattctctat aaagcggttc atgggcgaaa
    11041 cctatgataa ggttgccaac gttgaggttc cacgggttcc ctataaagtt gtccgtggcg
    11101 aaaacaacac gccgcgggtt gatattgatg gtcgcctata cactcctcag gaaatttcgg
    11161 ccatgattct tcaaaagatg aagaaaacgg cagaggacta cctgggtcag gaagttacag
    11221 aagctgtaat cacagttcct gcttacttca gcgactcgca acgtcaggct acaaaggaag
    11281 ccggtgaaat tgccgggctg aaagttcgac gtatcatcaa cgagcctacc gcagcagctt
    11341 tagcctatgg gctcgacaag aaaaacaaag atcttaaggt ggccgttttt gacctgggcg
    11401 gtggcacatt cgatatttca attctagagc tgggcgatgg ggtttttgag gtaaaatcaa
    11461 ccaacggtga cacccacctc ggaggtgatg acttcgacca tgtgattatt gactggctgg
    11521 ccgaggaatt ccttaaggat gagggtatcg atctgcgaaa agaccccatg gcactccaac
    11581 ggctgaagga ggctgcagag aaagccaaga ttgaactttc cagcaccacc tcaaccgaaa
    11641 ttaaccttcc ctacatcatg ccggttaacg gcatccctaa gcacctggtt aaaacactta
    11701 cccgtgcaca atttgagaag ctaaccgacc atttgttcca aaaaactatt gaaccctgtc
    11761 gtaaggcctt ggcagacgcc ggcttaaaag cttctgaaat tgatgaggtt atcctcgttg
    11821 gtggttccac acgtattcct tacgttcaac agcttgtgga aaatttcttt ggacgcactc
    11881 caaacaagag cgtaaacccc gacgaggtgg tggccgttgg cgcagccatt cagggtggtg
    11941 tcctcacggg tgaagttaag gatgtgctcc tgctcgatgt tacaccactt tcgctgggaa
    12001 ttgaaaccct tggaggcgtg ttcaccaagt tgattgaagc aaatacgacc attcctacaa
    12061 agaaatcgga ggtgttcacc acggcagccg acaaccaacc ctcggtagaa attcatatcc
    12121 tgcagggcga gcgtccgttg gccaaagaca acaaaaccat aggaaggttc cacctcgatg
    12181 gcttaccacc cgctccaaga ggcatacctc aaattgaggt aaccttcgat atcgacgcca
    12241 acggaatact gcacgtaacg gcaaaggata agggaaccgg taaggagcag aaaatcagaa
    12301 tcgaggcttc ctccggactc tccgacgacg aaattaagcg tatgcgcgag gaggcaaaag
    12361 caaacgaagc agccgaccgt gaagccaagg agcgtattga aaagctgaac aacgccgaca
    12421 gcctgatttt ccaaaccgaa aagcagctta aggaatttgg cgataagctt cctgcaagca
    12481 agaaagaacc cattgagcga gctctaagca agctaaagga tgcccataag aaccaggatg
    12541 ttagcgccgt tgaagttgca tctaacgagc ttaacgctgc atggcaggct gcctcagagg
    12601 aaatgtataa agcctcacag gaaaccgccg ggcaacaagc taaccctggt gccggaactt
    12661 catcatcatc atcttcctca tcaaccggca gcaacgacgc tgaggtaacc gatgtggatt
    12721 ttgaggaggt aaa
//
LOCUS       DAOPHX010000072        12718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_5826_length_12718_cov_5.621970, whole genome shotgun sequence.
ACCESSION   DAOPHX010000072 DAOPHX010000000
VERSION     DAOPHX010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 12718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12718
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5826_length_12718_cov_5.621970"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PK502_06605"
     CDS             <1..304
                     /locus_tag="PK502_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPH00522.1"
                     /translation="NPTSLRLATGMEFDLHSSLAEFLECFARRYELLSDEQSRFQLDE
                     YYLKKLYRRDLYHGYCDKNGNFKAKIAGVKPTGELVLQLPTGELRVYAFKEVMFLI"
     gene            412..1614
                     /locus_tag="PK502_06610"
     CDS             412..1614
                     /locus_tag="PK502_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPH00523.1"
                     /translation="MKRSVFAACFIVTALFSQLLFGQEPQKESESYVFTPIKELKVTP
                     VKNQNRSSTCWSFSGVAFLESELLRMGKPEVNLSPMFVVRNAYVEKADRFVRFNGTNN
                     FGPGGSFFDVVEMVKKYGIVPMEVYPGLNYGEDNHVHGELDAVTRSFVDEIVKNPNKK
                     LSTGWKPAFIGIVDGYLGQVPASFTYNGKTYTPITFAQSLGINLDDYVCIGSYTHHPF
                     YSKFVLEIPDNWIHGEVYNLPINEFERVLDNAIDNGYTIAWGSDVSEKGFSWRNGVAV
                     VPDINSAENAGSDKDHWVQLSQKEKEAKVYSFEKPLKEMVITQELRQQAFDNYQTTDD
                     HGMLIMGKAKDQNGNIFYKVKNSWGSEGKYNGFFYASKPFVLLKTTDIMVHKNAIPDD
                     IARKLNLK"
     gene            1871..2251
                     /locus_tag="PK502_06615"
     CDS             1871..2251
                     /locus_tag="PK502_06615"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPH00524.1"
                     /translation="MAELSSLYIKGTSKTPQIDFKPGMLQVSGRSIPEDAVEFFQPVI
                     GWLEEYLKSPEPLTRLNLRMEYINSGSNRFIFNLIKMLDDAYLKGHSVLVNWYYEEDD
                     DTIRNLGRDFQALVKVPFKMVEIV"
     gene            2388..2546
                     /locus_tag="PK502_06620"
     CDS             2388..2546
                     /locus_tag="PK502_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPH00525.1"
                     /translation="MYKCRICGYIYNPDEGDPASGIPAGTPFEELPESWHCPICYATI
                     EYFDELPE"
     gene            complement(2586..4040)
                     /locus_tag="PK502_06625"
     CDS             complement(2586..4040)
                     /locus_tag="PK502_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HPH00526.1"
                     /translation="MLSSLEPNNLWKFFEEICKIPRPSKKEKLVIAYLQNFAREHGLK
                     VRTDHAGNVLVEKPAYPGFEKLKPVVLQCHMDMVCEKNSDVAHNFQTDPIVPGIDGEW
                     VKANGTTLGADDGIGIAAQLAILESTEIKHGPLECLFTVDEETGLTGANELEKNFFSG
                     KILINLDSEDEGELFIGCAGGVDTLATFAYDTEKVPTNSTAFRLTVKGLKGGHSGDDI
                     DKGLGNSIKIINRFLWRGVNSYDLRLSMLDGGNLRNAIPREAYAIFTIHEEDRAALRK
                     EFTLIKNDILAELHDVEPDLSITFENADLPPYVVDEPTQYDLLNALYACPHGVISMSR
                     QLKGLVETSTNLASVKFIQDNQILVTTSQRSSVESAKKDISNMVESVFRLANARIQHS
                     DGYPGWTPNTNSEILGITRNSYIRLFGVEPVVRAIHAGLECGLFLEKYPELDMISFGP
                     TIKAAHSPYERLNIPSTMKFWKLLLDVLQNIPSI"
     gene            4282..5121
                     /gene="cysQ"
                     /locus_tag="PK502_06630"
     CDS             4282..5121
                     /gene="cysQ"
                     /locus_tag="PK502_06630"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HPH00527.1"
                     /translation="MIEQPEIINLLVTAINTSVRAGSKIMEVYNSDDFQVNLKSDRTP
                     LTLADRVAHEEIKNTLYKTLIPVLSEEGRSIHYVERKGWEYFWIVDPLDGTKEFIKRN
                     GEFTVNIALMYEGFPILGVIYVPVTECLYYSIHPEGSFKVTGIKPTMEVNLTYSDVCS
                     GAKRLPLSQNHSGIVVVESRSHNSPETLEFISNLDKKYGGITRISCGSSLKMCMVAEG
                     IADIYPRLSLSSEWDTAAGQAIVEGAGFQVISFDSGDRMSYNKEELINPWFIVRNPAR
                     FEV"
     gene            complement(5118..7454)
                     /locus_tag="PK502_06635"
     CDS             complement(5118..7454)
                     /locus_tag="PK502_06635"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPH00528.1"
                     /translation="MKALKRVALPVIVGFLAVLGTAAAPLATDSLARILNQAGQDTAS
                     LNAVFKKLISVDLPNPTQTDSLLNELLSRFKKLNYNDGVNRVLSFRGDLYYQQGNFQQ
                     ALEVEKELASKYGGSKTNMLPLANAYKMIAGCFFNMGIHDSAVVYAEKSISLFKNLND
                     SQNVARSLNLLGGIYWNMGNLTKASEKLYESLRLREQLNDSLGMANAYNNIGLIYDSQ
                     GKLTEAMKMYESALEIYNKLNNSKGIGRVCNNMAIILKNQKKFSESLEMFLKSLEVDK
                     KLNNIDDQGKTLNNIGQLYIEMGNIQAAIGYYQQARQLFLRSGNENGLAATLLNMGKA
                     SYDQKNYAESRTYYLQSLEVAKRINSTEWIRDSYHGLYLLAKQAGRASEALTLFERYK
                     SLDDSLRGLENMNKIDQLRVEFETEKKEREITLLTKDNELKTEKLNRQKTYNLLLLTI
                     TIAVVAFLGLTALAIKRLRADKKILVDKNQEIQAQKEEIESQRDLLEVTNTELNQQKE
                     ELQAQTDQIERQNYIIASANQRMTEGLEYASLIQKSLSSDINSFGKHFSGYSLLYMPK
                     DIVSGDFFWVWDENRDDNFTFAIADCTGHGVAGAFMSLMAINLLRDAVGVNNITEPQK
                     ISTFLYNELIRKKASIFDSNLIIGLDFIVCSYSPKSQVLCYSGNHLNFYLVREGEVNK
                     LKVERNIDRQSATLIFAQSSIKLKKNDRLYFTTDGFADQISETKRKKIGNSDFQNLLL
                     SVGNYPIKQHSELLAEYFSKWKGTYEQVDDVLVLGLEV"
     gene            complement(7611..9056)
                     /gene="dnaA"
                     /locus_tag="PK502_06640"
     CDS             complement(7611..9056)
                     /gene="dnaA"
                     /locus_tag="PK502_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPH00529.1"
                     /translation="MNVLDFKEVWQNCLNIIRDNVPPISFKTWFEPIVPVRLEQNVLT
                     IQVPSPFFYEYLEEQYIDILRKTLRKELGSDAKLEYSVVMEHGGYNNGTPYTVKFPTQ
                     NKTEIKNKPVSIQNLQEPPIKNPFIIPGIKKLHVDPQLNQEYTFANFLEGDCNRLARS
                     AGMAISQKPGNNPFNPFFIYGNSGLGKTHLAQAIGIEVKQKFPEKTVLYVNAAKFQTQ
                     FVDAHIKNNNINDFIHFYQSIDVLILDDVHEFAGKEKTQDAFFHIFNHLHQTGKQLVL
                     TSDQPPAELQGLEQRLLSRFKWGLSADLQTPDFATRVAILKRKVYNDGIVIPEEVIEY
                     IASNITQNIRELEGALISMLAQSTLNGREITIELASEMIEKLVKNPARKDLSIDYIQK
                     VVCEYFGLPVDVLQAKTRKREIVQARQIAMFFSKNLTKSSLSTIGAVIGSKDHATVLH
                     ACKTINNLMETDKRFKAQIEDIEKRIKYSNQ"
     gene            complement(9392..10387)
                     /locus_tag="PK502_06645"
     CDS             complement(9392..10387)
                     /locus_tag="PK502_06645"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPH00530.1"
                     /translation="MKIKLTKEAKIGIFMVACLFAMFWGINFLKGKNVFSSNNTYYVV
                     FTSVDGLKPTNDVLINGFKVGLIEEIEFNDIKSGKFKVNLLISKKYKLPRNTIAKLIS
                     ADLMGGKAIKLEISSDTAYYQPGDTLPGVIEVGLIDQLGHQMTPVKEKAEQLMVQLNN
                     TLLVVEKVFNEENRDQLSQGIANLNASLNNISQISAALDTMLNSPKGSLKTTFSNIES
                     VSSNLKKSNKELTNIIQNVSSVSDSLADINLAQTFTKLETTLNDIQAIIAKVNSGEGT
                     LGNLVSNDSLYTNLQKVTLSLDLLINDIKNNPKRYVSFSLIDLNRSQSSGEKVKE"
     gene            complement(10589..11767)
                     /locus_tag="PK502_06650"
     CDS             complement(10589..11767)
                     /locus_tag="PK502_06650"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPH00531.1"
                     /translation="MEGLRLKHFLPIPLLVVLSLLLAVGEKALSQTSSSYQLTRVVVD
                     AGHGGKDPGTVGRVSKEKDIALSIALKVGSYIEKNFPEVKVIYTRNDDTFIPLDERSR
                     IANEAKADLFISIHCNSAPNRTSFGTETYVMGLHRSADNLDVAMRENAVISYEDDYST
                     RYEGYDPSSSESYIIFSMLQNAFLDQSLSLANLIQTDFRDRASRKDRGVKQAGFLVLW
                     KTSMPSVLVEVGYLSNASEEKYLNSQEGQDYIASSIYRAFKEYKKNVDSYSNQLAQIQ
                     LTKPADSSVEQKGNTQVQPPSSTSQSHDFPIFKVQLMASNKPIPLDSPNFQNIKTVEE
                     LIINDSYKYCVGSLSSYDEALKLCREVRKQFPDAFVIATKNGVPVALQQAIKDNKTKN
                     "
     gene            11925..>12718
                     /locus_tag="PK502_06655"
     CDS             11925..>12718
                     /locus_tag="PK502_06655"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HPH00532.1"
                     /translation="MPLQDTVPATDTIKPAAGKSVLEDPVISNAEDSITYSIDGQTAY
                     LYGNAVVTYQNLELKAKYIEFNMATKQVYACGLPDSTGKVVGRPVFKEGGQTYEMDEI
                     HYNFDTKRAKITGVVTEQSGGFMHSKVTKMLEDQVVNLQDGKYTTCDLDHPHFYIAIT
                     KGKMIPDKKIIAGPAYLVIEDVPLPIGIPFGFFPNTRKRQAGVILPEYGEENIRGIFL
                     RGGGVYFGMGDYMDEKLTGDYYSKGSWGLRSQTTYKVRYRFSGTISL"
BASE COUNT         3215 a         2630 c         2958 g         3915 t
ORIGIN      
        1 caacccaacc tcgctaaggt tagctaccgg gatggagttc gatctgcact cctcgctcgc
       61 agaatttctg gaatgttttg cccggcggta cgaattgctt tccgatgagc agagccgttt
      121 ccagctggac gaatactact taaagaagct ttaccggcga gatttgtacc atgggtactg
      181 cgacaaaaat ggtaacttta aggctaaaat tgctggtgta aagcctacgg gggagctggt
      241 cttgcagctt cctacgggtg agttgagggt atatgccttt aaggaggtta tgtttcttat
      301 ttaaacgtgt tagtaattta ctgtctggct acttttaaac ttttttttaa atccggaatc
      361 tattaaacta gctaagcaat gtttaacatg aacattaaac tgtatgaaac tatgaaaaga
      421 tctgtatttg cagcgtgttt tattgtaacg gcccttttta gccagctgct ttttggccag
      481 gagccacaaa aggaatcgga aagttacgtt tttaccccaa ttaaggagct taaggttaca
      541 ccggttaaga accagaatcg ctcaagtacc tgctggagct tttcaggtgt tgcctttctg
      601 gaatcggagc tacttcgcat gggtaaaccc gaagttaacc tttcgcccat gtttgtagtg
      661 agaaatgctt acgttgaaaa ggctgatcgc tttgtcaggt tcaacggcac caataatttc
      721 ggacctggag gttcgttttt cgacgtggtt gagatggtaa aaaagtatgg tattgttccc
      781 atggaggttt atcctggctt gaactacgga gaggataacc atgttcatgg tgagcttgat
      841 gcggttaccc gttcttttgt tgatgagata gttaagaatc ccaacaaaaa actttccacc
      901 ggttggaaac ccgcattcat tggcatagtt gatggctacc ttgggcaggt tcccgcatcg
      961 tttacctaca atgggaaaac ttacacaccc attacttttg cacaatcgct tgggattaac
     1021 ctcgatgact acgtatgtat tggttcgtac acgcaccacc cgttctacag caagtttgtt
     1081 ctggaaattc ctgacaactg gattcatggc gaggtttata atttgcccat taacgaattt
     1141 gaaagggtgt tagataacgc aattgataat ggttacacca ttgcatgggg tagcgacgtg
     1201 agcgaaaagg gttttagctg gcgtaatggc gtggcagtgg ttcccgatat aaacagcgct
     1261 gaaaatgcgg gttccgataa ggatcattgg gttcaactct cccaaaagga aaaggaggct
     1321 aaggtttact cctttgagaa acctctaaag gagatggtta ttacccagga gctgcgtcag
     1381 caggcctttg ataactatca aacaaccgat gatcatggta tgctgattat gggcaaggct
     1441 aaggaccaga acggcaacat cttttacaag gtgaagaata gttggggatc agagggtaag
     1501 tataacggtt ttttctatgc ttctaaaccc tttgtgctgc ttaaaactac cgatattatg
     1561 gttcataaaa atgccattcc cgatgatatt gcccgcaagt taaacctcaa gtagcccttt
     1621 agttgtgggc aaattctttt gtctattctt gatgaagcca gatagtatgc attacctggc
     1681 ttcatcacta ttatgttttc gttaccttag gtttaggaaa gacagctttt ccctgaaaac
     1741 tgttcactct gtttaatgtt ggcgattatg ccggatgatg ggttaatccg atgacgtttt
     1801 tttaccaaaa atttttaaag tgggcatatt tttagctaaa tttacctcaa catataaatg
     1861 ctaaactgcc atggccgaat taagcagcct ttatattaag ggtacatcca aaacccctca
     1921 gattgatttt aagcctggca tgctacaggt ttcgggaagg tctataccag aagatgccgt
     1981 tgaatttttt caaccggtga ttgggtggct ggaggagtac ctgaaaagtc ccgaaccact
     2041 taccaggctt aacctgagaa tggagtacat aaacagcggc tcgaatcgct tcatttttaa
     2101 ccttatcaag atgctcgatg acgcctattt aaaaggccat agcgttttgg tcaactggta
     2161 ctatgaggag gacgacgata ccattaggaa tcttggccgc gattttcaag ctctagttaa
     2221 ggttccgttt aaaatggtcg aaattgttta ggtatggcct ccatttaacg tgttaatgaa
     2281 aactcctttg agaaaatttt gaatgctggt atgcaagctt atttcagtaa ggattagggg
     2341 tttactgtat ttgccacaca actttaattc ataaataccc tgcgttgatg tataaatgta
     2401 gaatttgcgg gtacatctat aatccggacg aaggcgaccc tgcctcgggc atacccgccg
     2461 gaactccttt tgaagagctg cctgaaagtt ggcattgccc tatttgctat gcaacaatag
     2521 agtattttga tgagcttccc gagtagctcc cgttgaagta ccgagttaca tagcgggtag
     2581 gctacctaaa tactcgggat attttgaagc acatccagca gaagcttcca gaacttcatg
     2641 gttgaaggaa tgttaagcct ttcgtaggga gagtgtgccg ctttaatggt tgggccaaac
     2701 gaaatcatat ccagctcggg atatttctca agaaataagc cgcattccag accggcatgg
     2761 attgccctta caaccggttc tactccaaaa agcctgatgt acgagttacg ggtaatgccc
     2821 agtatttcag agttggtgtt gggagtccat cctgggtagc catcggaatg ctgaattcgg
     2881 gcattggcca gccggaaaac actttcaacc atgttggaaa tatccttttt ggccgactcc
     2941 actgagctgc gctggcttgt ggttacaagt atctggttgt cctgtataaa tttcaccgaa
     3001 gccaggttgg tagatgtttc aaccaaccct ttaagctgcc tactcatcga aataacccca
     3061 tgtggacagg catacagcgc gtttagcagg tcgtattggg tgggctcatc aaccacatag
     3121 ggaggtaggt cggcgttttc aaaggttatg gataaatcgg gttcaacatc atggagctcg
     3181 gctagaatgt catttttgat gagtgtaaat tcttttcgta gggcagccct atcctcctcg
     3241 tgtatggtaa aaattgcata ggcctcacgg ggaatggcat ttctaaggtt tccaccatct
     3301 agcatggata gccgtaggtc gtagctgtta accccccgcc agagaaaacg gttgataatc
     3361 ttaatagagt tgcccaagcc cttgtcaatg tcgtcacctg aatgccctcc ctttagcccc
     3421 ttcacggtta gcctgaaagc ggtagagttg gtgggtactt tttcagtgtc gtaggcaaag
     3481 gtagccagag tatcaactcc accggcacac ccaataaata gttctccctc atcctccgag
     3541 tcgaggttaa tcagaatttt accgctaaaa aaattctttt cgagctcgtt ggctcctgtc
     3601 aaacctgttt cctcgtcaac agtaaaaagg cattccagcg gtccatgctt aatttcggtt
     3661 gattccagta tggcaagctg ggcggctatc cctattccgt cgtcggcgcc caaggtggtt
     3721 ccattggcct taacccattc tccgtcaatg cctggcacaa ttgggtcggt ttgaaagtta
     3781 tgcgccacat cgctattttt ttcgcaaacc atgtccatgt ggcactgcag cactacgggc
     3841 ttgagttttt caaatccggg ataggcaggt ttttctacta gcacgtttcc ggcatggtcg
     3901 gttcttactt taaggccatg ctccctggca aaattttgaa ggtaggcaat gaccagcttt
     3961 tccttttttg atgggcgggg aattttacag atttcctcaa agaatttcca aaggttgttg
     4021 ggttcaagcg agcttaacat ggtgatatgg gttaaaagtt aggtattgtt atgtcttaaa
     4081 attttgggta aacataacaa tttttccaat tttgaggaac cttagcaaac atattgtaaa
     4141 acatgaattg gtaagcggtg gaaggaagtt tctttatgct tattttttaa tgaataaaaa
     4201 tgttaaatgg ggttggtggg gaagggtata ttttttatta ttcaaggaaa gtgagctacc
     4261 tttgcagcaa gcaactaggt catgattgaa cagccagaga tcataaacct gctggttacg
     4321 gctataaata ccagcgtaag agcaggttca aaaatcatgg aggtttataa ctccgatgat
     4381 ttccaggtta acctcaagtc tgatagaaca cccctcacct tggccgacag ggtggctcac
     4441 gaagaaataa agaatacgtt gtacaaaact ttaataccgg ttcttagcga ggagggtaga
     4501 agcattcact atgtggagcg aaagggttgg gagtactttt ggattgtaga tcctctggat
     4561 gggacaaagg agtttatcaa gcgcaacgga gagtttactg taaatattgc cttaatgtat
     4621 gaggggtttc ctatcctggg cgttatctat gttcctgtta ccgagtgcct ttactattcc
     4681 attcaccccg agggttcgtt taaggttaca ggtatcaagc caacaatgga ggtgaattta
     4741 acctactctg atgtgtgctc cggggccaag cgcctaccac taagccaaaa tcatagcggt
     4801 attgttgtgg ttgaaagccg gtcgcacaac tcgcccgaaa cgctggaatt catttcgaac
     4861 ctcgataaaa agtacggtgg cattacccgt atttcgtgtg ggtcttctct taaaatgtgt
     4921 atggtagccg aagggatagc cgatatttat ccgcgtttat cgctttcctc tgagtgggat
     4981 actgctgctg gtcaggctat agttgagggt gctgggtttc aagtaatttc gttcgattcg
     5041 ggagatagaa tgtcctacaa taaagaagag ctgataaatc cctggtttat cgttcgtaat
     5101 ccagcccgct ttgaggttta aacctctaac cccagaacta atacgtcgtc aacctgttcg
     5161 taggtaccct tccattttga gaagtactca gcgagtaact cagaatgctg ttttatggga
     5221 tagttaccta ctgagagtag aagattctgg aaatcggaat ttccaatttt tttccttttg
     5281 gtttcgctta tctggtcggc aaagccatcg gtagtgaagt aaagcctgtc attctttttg
     5341 agcttaatgg atgactgggc aaaaatcagg gtggccgatt gtctgtcaat atttctctca
     5401 acctttagct tgttaacctc cccctctcgc accaggtaga agttaaggtg atttcctgag
     5461 tagcagagta cttggctttt aggggagtag ctgcaaacaa taaagtcgag tccgatgatc
     5521 aggttggagt caaaaataga ggcttttttt ctgattagct cgttgtaaag gaaggtagag
     5581 attttctgtg gctccgtaat gttgttcacc ccaacggcat ccctaagcag atttatagcc
     5641 atcaggctca tgaaggcacc ggctacacca tgtccggtgc aatcggcaat ggcaaaagta
     5701 aaattatcat cacggttttc atcccacacc cagaaaaagt ctccgcttac aatgtccttg
     5761 ggcatgtaga gcagggagta tccactgaaa tgcttaccaa acgaattaat gtcagaggat
     5821 agggatttct gtattagcga ggcgtactcc aaaccttctg tcatcctttg gtttgccgat
     5881 gctatgatgt agttttgcct ctctatttgg tcggtttggg cttgtagctc ttccttctgc
     5941 tggtttagct ctgtgttggt aacctcaagc aggtcgcgtt gagattctat ttcctccttc
     6001 tgagcttgta tttcctggtt tttatctact aatatttttt tatcggctct taaacgtttt
     6061 atggctaaag cggttagccc aagaaaagct actacggcaa ttgtgatggt gagtaaaagg
     6121 aggttgtatg ttttctggcg gtttagcttt tcagttttaa gctcattatc cttggttagc
     6181 aaggtaattt cacgttcttt cttctcagtt tcaaattcaa ctcggagttg gtctatctta
     6241 ttcatatttt ccagcccgcg cagggagtca tcaagcgatt tgtaacgttc aaataaagta
     6301 agggcttccg atgcacgtcc agcttgcttg gcaagcaggt aaagcccatg ataggagtct
     6361 ctaatccatt cggttgaatt tattcgtttg gcaacttcca ggctttgcag gtagtaggtt
     6421 cgtgattcag cgtaattttt ctgatcatag gaagctttcc ccatgtttag gagcgttgca
     6481 gccaacccgt tttcatttcc gctcctaagg aatagctgcc tggcttgctg gtagtatcca
     6541 attgccgcct gaatgttgcc catttcaatg taaagctgac ctatattgtt gagggttttg
     6601 ccctggtcgt caatgttgtt caacttttta tccacctcaa gggattttag gaacatctca
     6661 aggctctcgc taaatttttt ctggtttttt aggatgatgg ccatgttgtt gcatacccta
     6721 ccaataccct tgctgttatt gagtttattg taaatttcca aagcactctc atacattttc
     6781 attgcctcgg tgagcttccc ttgtgagtcg tagattaggc caatgttgtt gtatgcatta
     6841 gccataccca gcgagtcgtt gagttgttcg cgcagccgaa gcgattcgta tagtttttcg
     6901 gaagctttgg tgaggtttcc catgttccag taaatacctc caagaagatt gaggcttcgt
     6961 gcaacatttt ggctatcgtt cagatttttg aaaagtgaaa tagatttctc ggcatacaca
     7021 acggcgctat cgtgaatacc catgttaaaa aaacacccgg caatcatctt gtaggcgttg
     7081 gccagcggca gcatgttagt ctttgaccca ccgtacttgc tggccagctc tttttcaact
     7141 tcaagtgcct gctgaaagtt accctgctgg tagtatagat ctcccctgaa agatagcacc
     7201 cggtttacgc catcgttgta gttaagcttc ttaaacctgc taagtaattc gtttaagagg
     7261 gaatcggttt gagtagggtt tggcaaatca acggaaatca gcttcttaaa aacggcattc
     7321 aatgaagcag tatcttgtcc ggcctggttt aaaattcggg ctaaactatc ggtagccaat
     7381 ggagcagctg cagtgcccaa cacagccaga aatcctacaa ttacaggtag agcaaccctt
     7441 tttaatgctt tcatggcaag ctgaaatcag ctttaaaatt aacttaagct tttctcccgg
     7501 ctggtaaacc ctacaacaat ttacaaagat agccttattt ggattaaaat gcagctgggt
     7561 gaattttaat caagccgatg tagtataggg ctgcttgtta ggttgtttct ttactggttg
     7621 ctatacttta tgcgtttctc aatatcctca atctgggctt tgaatctttt gtcggtctcc
     7681 attaggttgt taattgtttt gcaggcatgg agaactgttg catgatcttt actgccgatc
     7741 actgcaccta ttgttgagag ggaggatttg gtgaggtttt tggaaaaaaa catggcaatc
     7801 tggcgagcct gcacaatttc tcgttttcgg gtttttgcct ggagcacatc tactggtaaa
     7861 ccaaagtact cgcacacaac cttttgtatg tagtctatgg acaaatcctt tctggcaggg
     7921 tttttaacca gtttttctat catttctgat gccagctcaa tggtaatttc cctgccgttg
     7981 agggttgact gtgcaagcat ggagatgagc gctccctcca gctcgcggat attctgggtt
     8041 atattggagg ctatgtattc aattacctct tcagggataa caatgccgtc gttataaacc
     8101 ttacgcttaa ggattgctac acgggtagcg aagtcgggtg tttgcaggtc ggccgagagt
     8161 ccccatttaa aacgggagag caggcgctgc tcaagcccct gtagctcagc ggggggttgg
     8221 tcggaggtga gtactagctg cttaccggtc tggtgcaggt ggttgaagat gtggaaaaag
     8281 gcatcctggg ttttttcctt tccggcaaat tcatgcacat cgtccagaat gagcacgtca
     8341 atactctggt aaaagtggat aaagtcgtta atgttgttat tttttatgtg ggcatctaca
     8401 aattgggttt gaaatttagc tgcgttaacg taaaggacgg ttttctctgg aaacttttgc
     8461 tttacttcaa tgccaatagc ctgtgccagg tgggttttac ccagtcccga gttgccgtat
     8521 atgaaaaaag ggttgaaggg gttattgcct ggtttttggg aaattgccat accggccgaa
     8581 cgtgccagcc ggttacaatc tccttcaagg aaatttgcaa aggtgtattc ctggttgagc
     8641 tgggggtcaa catggagttt ttttataccg gggattataa aaggattttt tattggcggt
     8701 tcttgtaggt tctgtatgga taccggttta ttcttaattt cggttttatt ctgagtgggg
     8761 aactttaccg tatagggggt gccgttgttg tacccgccat gctccatcac tacgctgtac
     8821 tccaattttg catccgatcc caactctttt ctcagtgttt tccggagaat gtctatgtac
     8881 tgctcttcca aatactcata aaagaatggg ctgggaacct gtatggtgag tacattttgc
     8941 tccagcctta ctggaactat tggctcaaac catgttttga agcttatcgg aggaacgttg
     9001 tctcttataa tattcaggca gttctgccat acctctttaa aatctaaaac attcattacc
     9061 atagctaaaa aaccttatga ttttttttta ctcttttcta tttcctcaac aaaattgtaa
     9121 tgaatttttt taaaaaaaaa ggtggttagg gcttgaattt ccctttaaca ttacaacagg
     9181 ttagctatca tggtaataat tttttttcat tcgtaaaaat tttttaatta gctaattaac
     9241 aattagatat catgttgata acgtaaaaat ttgaatgtta actatttgtt aatacaatat
     9301 taagcttgtt gtttacccag ctgcactgta actattcaac cctgtgcatg ttggggttaa
     9361 catgcagata ttaagatgtt tacgtttcag gctattcttt aaccttctcg ccggaggatt
     9421 gcgacctgtt tagatcgatt aatgagaagc taacatacct tttggggttg ttcttgatgt
     9481 cgttaataag caggtcgagg ctcaaagtta ccttttgcag gttggtgtaa aggctgtcgt
     9541 tgctgacaag gttgccaagg gtgccctctc cggagtttac tttagctatt atagcctgga
     9601 tgtcattcag ggtggtttcc agctttgtga aggtttgtgc caggttgatg tcggccaatg
     9661 agtcgctcac cgacgacacg ttttgaataa tgttggtaag ctccttgttg cttttcttga
     9721 ggttactgct aaccgattcg atgttgctaa aggtggtttt gagcgagccc ttaggggagt
     9781 tcagcatggt gtcgagtgct gcgcttatct ggctaatgtt gttgaggctg gcattcaggt
     9841 tagctatacc ctgtgatagc tggtcgcgat tctcctcgtt gaaaactttt tcaactacca
     9901 gcagcgtatt gttcagctga accatgagct gctcagcttt ttctttaact ggggtcatct
     9961 ggtggcccag ctggtcaatc aggcctacct caatgactcc aggtagggta tcgccgggtt
    10021 ggtagtaggc cgtgtcggac gaaatttcca gcttaatagc cttaccgccc attaaatcgg
    10081 cgctgattag cttggcaatg gtgtttcgag gcaacttgta cttcttgcta atcaaaaggt
    10141 taaccttaaa cttccccgat ttgatgtcgt tgaactcaat ttcttctatc aggccaacct
    10201 taaacccgtt gatgagaaca tcattggtgg gttttagccc atcaaccgat gtaaaaacta
    10261 catagtatgt attgttggag ctgaacacgt ttttcccctt taaaaagttg ataccccaaa
    10321 acatggcaaa caagcaggcc accatgaata tccctatttt tgcttctttg gtaagtttta
    10381 ttttcatttc taaagtagta ttaacattta actagctggc agatggcaaa gatacacaac
    10441 cacatcaatc tggggtaatt ttctcttcac gttgcccgcg gctatttaaa atatttattg
    10501 ctattgtttg taatggtgat tagtgttagc tggttctgta cgtgtagtca gtttatacct
    10561 gaataagggt aagtaaaatt tccacctatc aatttttggt tttgttatcc ttaatggctt
    10621 gctgtagcgc tacaggaact ccattttttg tggcaattac aaatgcatcg ggaaattgct
    10681 tacgcacttc cctacagagt ttaagggctt cgtcgtagga gctcaggctg cctacgcagt
    10741 acttatagga atcgtttatg ataagctctt caactgtttt aatattctga aaattaggtg
    10801 aatcgagggg gattggcttg tttgaggcca ttagttgcac cttaaaaatt gggaaatcat
    10861 ggctttggct tgtgctggat gggggttgaa cctgggtgtt gcctttctgt tcaacgctgc
    10921 tgtcggctgg cttagtgagc tgtatttgtg ccagctggtt gctgtagcta tccacgtttt
    10981 tcttgtattc cttaaaggct cggtaaattg aggaggcaat gtagtcctga ccctcctggc
    11041 tattcaggta cttctcttcg ctggcgttgc tgaggtaacc cacttcaacc aggacgcttg
    11101 gcatagaggt tttccatagc acaaggaacc cggcctgctt tacccctctg tcctttcggc
    11161 tagccctgtc tctaaagtca gtttgaatca ggttggcaag cgagaggctt tgatcaagaa
    11221 aggcattctg aagcatggag aagattatgt acgactcgga agaacttggg tcgtaaccct
    11281 cataccttgt cgagtagtca tcttcatacg aaattacagc gttttcacgc atggcaacgt
    11341 ccaggttgtc ggccgatcgg tgcaacccca tcacgtatgt ttcggtgcca aaagaagttc
    11401 tgtttggggc ggaattacaa tgaatggaga taaagaggtc ggcttttgct tcattggcaa
    11461 ttctactcct ctcgtctaac ggtataaagg tgtcgtcgtt tctggtgtag attaccttca
    11521 cctcagggaa gttcttttca atgtagcttc ctacctttag agcaatgcta agggcaatgt
    11581 ccttttcctt gcttacccgc ccaactgttc ctgggtcctt acctccatgc cccgcatcaa
    11641 ctaccaccct ggtgagctga taggagctgc tggtttggct caaagctttt tctccaaccg
    11701 ccagcaagag ggatagaacc accagcaagg gtattggtaa aaaatgtttt aagcgcagcc
    11761 cctccattat tccctaaaaa ttttttagta gttttgagct caattttgtt acaaaaatac
    11821 gactaaaatt gaaattcagg gtaacaacgc tttttattgt tttatatagc tcattagggt
    11881 ttgctaggca ggccatacct ttttcgccca attttgtatt gcaggtgcca cttcaggata
    11941 cggtgcccgc taccgataca attaaacctg cagctggcaa aagcgtgctg gaagaccctg
    12001 tgataagtaa tgctgaggat tctattactt actctattga cgggcaaacg gcctacctct
    12061 atggcaacgc tgtggtaacc taccaaaatc tggagctaaa ggcaaagtac atcgagttta
    12121 acatggcaac caagcaggtt tacgcctgcg gtttgcccga tagcacgggg aaagtggttg
    12181 gtcgtccggt gtttaaagag ggtggccaaa cctacgagat ggatgaaata cactataact
    12241 tcgatactaa gcgggctaag attactggcg tggttaccga gcagtctggt ggtttcatgc
    12301 acagcaaggt aaccaagatg cttgaagacc aggtagtaaa cctgcaggat ggtaagtaca
    12361 ccacctgtga tttggatcat ccacattttt acatagccat aaccaaggga aaaatgattc
    12421 ccgataaaaa aattattgcc gggcctgcat acctggttat cgaggatgta ccgctaccaa
    12481 ttggcattcc ctttgggttc tttcccaaca cccgaaaacg gcaagcaggg gtaattctcc
    12541 cagagtatgg cgaggaaaat atcaggggaa ttttcctgag gggtgggggt gtttactttg
    12601 gcatgggcga ctacatggac gagaagctta cgggcgatta ctactcaaag ggctcatggg
    12661 ggttacgcag ccaaaccacc tacaaggttc gctaccggtt ttccggcact ataagcct
//
LOCUS       DAOPHX010000073        12623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_5899_length_12623_cov_6.060869, whole genome shotgun sequence.
ACCESSION   DAOPHX010000073 DAOPHX010000000
VERSION     DAOPHX010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 12623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12623
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5899_length_12623_cov_6.060869"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1892
                     /gene="ggt"
                     /locus_tag="PK502_06660"
     CDS             219..1892
                     /gene="ggt"
                     /locus_tag="PK502_06660"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPH00533.1"
                     /translation="MLLLLALVYFTGSGQDRMTGLNFATRSEVIASHGMACTSQPLAT
                     QVAIDILKKGGTAVDAAIAANAMLGLVEPTGSGIGGDLFAIVWDAKTQKLYGLNASGR
                     SPKSLSLEYFKEHGIKHIPAIGPLPVSVPGCVDGWFELHSRFGRLPMKVILEPTIGYA
                     REGFPVSELIAYYWKGNAERLKQFEGFADVYLPNGKAPAKGEIFKNPYLANTLEMIAK
                     NGRDEFYRGGIARTIDDYMKRNGGFLSYNDLASHRSEWVEPVSTSYRGYDVWELPPNG
                     QGIATLEMLNILENFDIASMGFGSADYMHLFVEAKKLAFEDRARYYADPDFTDVSYMS
                     LLSKDYAKNRAALIDMNRAARSYDAGELQTGNTVYLTVADSEGNMVSLIQSNYRGMGS
                     GMTPTGLGFVLQDRGELFSLEEGHPNVFQPGKRPFHTIIPGFITKSGRPWVSFGVMGG
                     GMQPQGHVQVIVNLIDFGMNLQEAGDAPRIQHEGSSEPTGERMNDGGNVFLESGFDYQ
                     EIRKLLGKGHRISYSLDGYGGYQAIMYDERNRVYVGASESRKDGQAAGY"
     gene            complement(1941..3143)
                     /locus_tag="PK502_06665"
     CDS             complement(1941..3143)
                     /locus_tag="PK502_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPH00534.1"
                     /translation="MLRKSVYALALGGLLLTACQKEENFSDETNPLSTKQELKNGDII
                     PGKFIVVFTQDTFSTRVDKVQQVSYEESHAKALAEASTFLSVYAVPSGKIDQVYGASI
                     KGFSAELNELQVNAIYRDSRVAYIEPDRWIVLDYKVESISDPTAKGQTTPWGITRVGG
                     ATNYTGTNVAWIIDTGIDLDHPDLNVDVSRSKTFVPRGRTPDDDNGHGSHVAGTVAAK
                     NNTIGVVGVAAGAPVVAVKVLDRSGSGAYSTIISGVNYVASAGKSGDVANMSLGGPTY
                     QALDDAVLAASNKGIKFSLAAGNESANANLSSPARVNGSNIFTISAMDNADKFAYFSN
                     YGNPPIDYCAPGVSIYSTYKSGKYATLSGTSMASPHACGVTLLGSPRTDGYVLNDPDG
                     NPDPIIHK"
     gene            3414..3914
                     /locus_tag="PK502_06670"
     CDS             3414..3914
                     /locus_tag="PK502_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HPH00535.1"
                     /translation="MVELVKGDITLMDVDAIVNAANESLLGGGGVDGAIHRAAGPQLL
                     AECRTLGGCPTGEARITRGYNLKARYIIHTVGPVWHGGSRGEAKLLASAYWNSLLLAA
                     DYSLKSIAFPNISTGVYGYPKREAAEIATRTVNEFKQQNLHPERVVFVCFDDDSYQIY
                     KEMLKR"
     gene            complement(3911..5293)
                     /locus_tag="PK502_06675"
     CDS             complement(3911..5293)
                     /locus_tag="PK502_06675"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPH00536.1"
                     /translation="MKEIRILVLDDEKVFRDEIKEFLENDGFTVLLSDRPSAAFEMLT
                     QQEVDIMILDIKLPEMDGFEVLRRVKEEYPDVEVIMITGHGDMEAVIQAMRLGAVEFF
                     PKPFRLLDMRAAIQRTKRYITISNQLKEINQTYSLISKELIESVGNEILGTSPAISHV
                     VNLMGRVAKTENTSVLITGESGAGKELVARGIHYLSNRKDSYFYAVNCSAIPETLFES
                     EFFGHKKGSYTGANEDKAGWFEVANGGTLFLDEIVEMLPAMQSKLLRVLEERKVRRIG
                     SSVDIPVDVRIIAATNQNVFTQLETGKLRSDLYYRLNTFEIHIPPLRERREDIPVLLD
                     YYVNFFARKLGKRISEIDPSVQRVMMGYNFPGNIRELRNMVERAIILCDGSKLSLKNF
                     PGLTSQTGEAGTQLPGIGDDVFDLDLIEKFVIMKALERTGYKKTEAAQLLNITRQSLD
                     RRLEKYSINI"
     gene            complement(5300..7543)
                     /locus_tag="PK502_06680"
     CDS             complement(5300..7543)
                     /locus_tag="PK502_06680"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPH00537.1"
                     /translation="MSRFGKHTTIVLAFWLFMQFPTLQAQVSPDSSLTSKSEEKVLAN
                     LISEGRDNVVKNPDKALDNGLQALVLAKKLGDTRFEIEALLILAESNFTLGQYENSSR
                     YYNRALNFYDQLGNSDAKYKILIGLGRVYDAEKNPELAISTLERGLASDENINNINLY
                     IDLLELLGELYQKQGNYRKSAEMYSKVLAIVDNPMGRKVLLKEKIKSDAFFKLGQVKK
                     NSGELVESLSNFKQAAEISINNFDSLSYSNALQEIALTYYLLQKPDSANFYFTASYAI
                     SNKISDTVGMIQGLQGIADIYLDSGNPTQALYYYNQQLTVAQKLKNIPAITESLVKIS
                     RCYLSSGDISNASKYLNRALAIARQNNLTQSVADVYQYLAILNEGQGRYRDALEFYKM
                     WVETRDSIYFEETGQKLAKLKILYEISQKEKENELLRQNSEIQNLQLNKTRYQTRILI
                     LIIIIFITLIVLLIILFRSKQKEIAKRLETEQRITDLNRELERRMIQEIKKQEKQQLL
                     LAQKSKLESLGTLAAGIAHEINQPLGGISMGLDNILLKVQDKSYNDEHMKEKIASLFE
                     NVERIKKIIDHIRYFSRAQKPITFTQININDVVNSSLFMVSTQYENHGVKMEVTLDEN
                     IGSITADKYKLEQVILNLLSNAKYAVDEKGKKHANHQYTKLIEIKTWQDNNQVYLSVR
                     DNGMGIPDKIIDKIFDPFFTTKSEERGTGLGLSVSYAFIKDLLGDIKVESVEGEYTRF
                     EISLPKM"
     gene            7898..8758
                     /locus_tag="PK502_06685"
     CDS             7898..8758
                     /locus_tag="PK502_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HPH00538.1"
                     /translation="MVKTIRNYLSLVKFSHTVFALPFAVIGFFLAYRQTPQNVDVWLF
                     VLVVLCMVFARNAAMSFNRLVDRRFDAINPRTQNREIPRGIITSRATAVFVTVNVLLF
                     IATTYFINRTVFFLSPIALAIVLLYSYTKRFTMFCHFVLGLGLALAPIGAYLSVTPRF
                     ELLPLLYSVIVLFWVSGFDIMYALQDEDFDKRENLKSLPAALGVKKSLLVANLLHFVA
                     GAVVVLIGILYHSDVWYWVGAALFIALLIYQHLIVKPTDISRVNMAFATTNGLASIIY
                     ATFNVISIFF"
     gene            8819..9271
                     /locus_tag="PK502_06690"
     CDS             8819..9271
                     /locus_tag="PK502_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00539.1"
                     /translation="MMKKVFLLAATAASILACSCGTKSSSDNQQAKADSTAASIVEVS
                     IDSLISNPQAFANQTVKFTAKVDHVCMHSGQKLTLMGSDSGSTFKVMATDAVPQFDQN
                     LNGTTVEVVGVVQGAAPEDVESCSEEEGGQQKAKKLDYAVFCQKVRAL"
     gene            9388..9930
                     /locus_tag="PK502_06695"
     CDS             9388..9930
                     /locus_tag="PK502_06695"
                     /inference="COORDINATES: protein motif:HMM:NF027675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HPH00540.1"
                     /translation="MRKLLRVLHRDFGYFFTGMTLIYCVTGILLNHTRDWNPYYSIDI
                     RKVELNLPLDTSLINKELLTSKLEPIGVQGLRSFYYPKPGTVKLFVSDGTVTSDLGSG
                     SALVERSYRRPVLYQLNMLHKNLPKSWWTWFSDIFAIALMAITISGLFLVKGKNGITH
                     RGVILIIAGLALPLVAIILL"
     gene            10189..11565
                     /locus_tag="PK502_06700"
     CDS             10189..11565
                     /locus_tag="PK502_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HPH00541.1"
                     /translation="MELPFAESYKIKMVEPIHRSTRSERERWIKEAKYNMFNLKSEQV
                     FIDLLTDSGTGAMSDRQWSALMMGDESYAGASSYYNLKSAIQNILGFEHFLPTHQGRA
                     AENVLFSVMVKEGDYIPGNSQFDTTKGHIEFRKAEAVDCTIDEAENTEIYHPFKGNVD
                     PQKLDDFIRTHADRVPFVIITITNNSAGGQPVSMQNLKDARKICDKYGKPLVIDSARF
                     AENAYFIKVREKGYADKTIKEIVREMFSYADAMTMSSKKDAIVNIGGFIAMRSKELWQ
                     QCSTFNIMFEGFVTYGGMSGRDMNALAVGLDEGTEFDYLETRIKQVEYLGNRLKDFGI
                     PFQFPAGGHAIFVDANKVLANVPREQFRAQTLAVELYLEAGIRGVEIGALMSDRDPVT
                     REVRMPKLELLRLAIPRRVYTNNHIDVVAVALKNVFDRRMDIKNGLQITWEAPIMRHF
                     TVQLDRAR"
     gene            11676..>12623
                     /locus_tag="PK502_06705"
     CDS             11676..>12623
                     /locus_tag="PK502_06705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPH00542.1"
                     /translation="MRDFTTGSETQHILRFATPMLIGDIFQQLYNVVDSIIVGRFIGD
                     KALAAVGASFPVIFVIIALVLGIGIGSTVVISQYFGARNFDMVKRAANTIYIFLLISG
                     VVVTAIGLMASGAIFRMLHLPEELMKPARTYLNIFMLGMVVMFAFNAIASILRGIGDS
                     KTPLYFLLISTLTNVGLVLLFVVKFRWGIAGAAWATVISQVVAFIIALIYLNHSQHML
                     SINLRTMKFDRQIFRQIVKIGLPTGIQQTMVALGSMAMMGIVNSFGTTVIAAFTVASR
                     LDALIATPIMDFAAALSGFVGQNIGANKIDRVKRGLRSTL"
BASE COUNT         3141 a         2773 c         3005 g         3704 t
ORIGIN      
        1 atccagggcg tgttttagca acaagtccag cccatcctct ttctcgctga tttctaccag
       61 aatttcgtta atttgcccct gtgtaaagtt taagtttttc attcttttaa gttttatggt
      121 ttgaaacttc aaatttatga aagacttgct ttacacactt tttgggacaa tatcactagt
      181 acatacaaat tgcgagtatt atgttgaaaa ggggtaaaat gttgctgtta ctggcgcttg
      241 tttattttac cggcagtggc caggatagga tgacagggct taactttgcc acccgttcgg
      301 aggtgattgc gagtcacggt atggcctgta ccagccaacc gttggcaact caggtggcca
      361 tagatattct gaaaaagggc ggaactgctg tggatgctgc catagcagct aatgccatgc
      421 ttggtctggt tgaaccaacc ggttcgggca ttgggggtga ccttttcgcc attgtatggg
      481 atgccaaaac gcaaaaactc tatgggttaa atgccagcgg ccgttcgccc aagtcgctat
      541 cacttgagta tttcaaggag catggaataa agcatatccc tgccattggc ccgctgccgg
      601 tatcggtgcc tggttgcgtg gatggctggt ttgagctgca cagtagattt ggcaggctgc
      661 ccatgaaggt aatccttgaa ccaacaattg gctatgcccg ggagggattt cctgtatcag
      721 agctaattgc ctactactgg aagggtaatg ccgaaaggct taagcagttc gaaggttttg
      781 ctgatgttta cctgcccaac gggaaagcgc ctgcaaaggg ggaaatattc aagaatccct
      841 acctggcaaa taccttggaa atgattgcaa agaatggtcg tgacgaattc taccgtggcg
      901 gcattgcccg gaccattgat gattacatga agaggaatgg tggatttttg tcctacaacg
      961 atttagcctc acaccgctcg gaatgggttg aacccgtttc aacctcatac cggggctacg
     1021 atgtttggga gcttccccca aacggccagg gcattgcaac gctcgagatg ctaaacatcc
     1081 tcgaaaattt cgatattgcc tcaatgggct ttggctcggc tgactacatg cacctttttg
     1141 ttgaagcaaa gaaactcgcc ttcgaggata gggcacgtta ctatgccgat cctgatttta
     1201 ctgacgtttc atatatgagt ctgctttcga aggactacgc aaaaaatcgt gctgccctta
     1261 ttgatatgaa ccgtgcagca agaagttacg atgcgggtga gttgcaaaca ggcaacaccg
     1321 tttacctcac cgttgccgac agcgagggaa acatggtttc gctcattcag agcaattaca
     1381 ggggaatggg ttcgggaatg actcctacag gtctgggttt tgtgctgcag gacaggggtg
     1441 agctgttctc gcttgaggaa ggacatccca atgttttcca acccgggaaa aggccatttc
     1501 acaccattat tcctggtttt attacaaaga gtggaaggcc atgggtaagc tttggggtta
     1561 tgggtggcgg aatgcaaccc caggggcatg ttcaagttat tgttaacctt atcgactttg
     1621 gtatgaactt acaggaggca ggcgatgcac cgcgaatcca gcacgagggt agctccgagc
     1681 caacgggtga gaggatgaac gatggcggta acgtttttct ggaatctggc tttgactatc
     1741 aggaaatccg taaacttctc ggcaaaggac acaggatttc atacagcctt gacggttacg
     1801 gcggatacca ggctataatg tacgatgaac ggaatagggt ttacgttggg gcttcggagt
     1861 cgagaaaaga tgggcaagca gcaggatact aatcaaaaaa gaggcttaaa gcctcttttt
     1921 tgattagtga cgcaatataa ctacttgtga atgataggat cggggttgcc atcgggatcg
     1981 ttgagcacgt atccatcggt tcggggtgag ccaagcaggg ttactccaca agcatgaggt
     2041 gaagccatag aagtgccgct aagggttgcg tatttacccg acttgtatgt cgagtagatg
     2101 cttactccgg gagcacagta gtctataggc ggattcccat agttggagaa gtatgcaaat
     2161 ttatcggcat tatccattgc tgagatggtg aagatgttag acccattcac ccgtgcagga
     2221 gagctaaggt tggcattagc actttcatta ccagcggcta acgaaaactt aatccccttg
     2281 ttagatgctg ccagcacagc atcgtcgagt gcctgatagg tggggcctcc taggctcatg
     2341 ttggctacat cacccgattt gccagcagag gcaacatagt taacaccgga aataatggtg
     2401 gagtaagctc cgcttccact acggtcgaga acctttacag ccactacagg cgctcctgca
     2461 gcaaccccaa caacgccaat ggtgttattt tttgcagcaa ctgtccctgc aacatgcgag
     2521 ccatgcccgt tatcgtcatc gggagttctt ccacggggta cgaaagtctt gctgcgagaa
     2581 acatccacat ttaaatcggg atggtctaaa tcaatacctg tatcgattat ccatgccacg
     2641 ttggttcctg tatagttggt agcgcctcca acacgggtta taccccatgg agtggtttgg
     2701 cccttcgctg tgggatcgga gattgactca accttgtagt ccagcactat ccagcgatca
     2761 ggttctatat aggcaactct cgaatcccta taaatcgcat ttacctgtag ctcgttgagt
     2821 tcggcggaaa aaccttttat ggaagctcca tacacctggt caatcttacc ggatggtaca
     2881 gcataaacac tcaaaaaggt tgatgcttca gccagcgctt tagcatggct ttcttcataa
     2941 cttacctgtt gcaccttgtc aacccttgtg ctgaatgtat cctgtgtaaa taccacaata
     3001 aatttccccg ggatgatatc gccattcttc agctcctgct tcgttgagag cgggttggtt
     3061 tcatccgaaa aattttcttc cttttggcag gcagtcagta gcaacccgcc aagcgccaga
     3121 gcataaacag attttcttaa catggttaaa tggtttaggt tagtattcag gtgcaaatta
     3181 tggctatatt acataaaagt caagtgctca gaaagtttta actatttttt tcggcatttg
     3241 acggatttaa atttcaaata atacgcagcc aatcacatag ctttaaacct ttaacgccta
     3301 ccggctcacg gctttcaact caattttttt tagcacaccc gggttacctg taagaacaat
     3361 ttcactatct tttaaccatc taaaatccat tttacttaaa acgataacag gttatggtag
     3421 agctggttaa aggagacatt acgttaatgg atgtggatgc gatagtaaat gccgccaacg
     3481 aatcgttgct tggcggtgga ggagttgatg gcgctatcca tcgtgctgcg ggaccgcagc
     3541 tacttgcaga atgccgaacg ttgggtggat gccctactgg tgaggcacgc atcactagag
     3601 gctacaatct taaagctagg tacataattc atactgtagg ccctgtttgg catggcggat
     3661 cgcggggtga ggctaagctt ctggcctcag cctattggaa cagcttactg cttgcagctg
     3721 attactcgct gaaaagtatt gcatttccta acattagcac cggtgtttac ggttatccca
     3781 aaagggaggc cgccgaaata gccactagaa ccgtaaacga attcaaacag caaaacctac
     3841 atcccgagag ggttgttttt gtctgctttg atgacgatag ctatcagata tacaaggaga
     3901 tgcttaagcg ttaaatgttg atggagtatt tctccagcct tcggtcgagc gattgacggg
     3961 tgatgttgag cagctgtgca gcctctgttt tcttgtagcc tgtgcgttcc agggccttca
     4021 taatcacaaa tttttcaatg aggtccaggt cgaatacgtc atcgcctatg ccgggtagtt
     4081 gggtgcccgc ttctccggtt tggcttgtta agcccggaaa atttttcagg ctaagcttgc
     4141 taccatcgca aagaataatg gcacgctcaa ccatgttgcg cagctccctg atgtttccgg
     4201 ggaaattgta gcccatcatt accctttgca ccgatgggtc tatttcggaa atccttttgc
     4261 ccagcttacg ggcaaaaaaa tttacatagt agtccagaag tactggaata tcctccctac
     4321 gttccctcag aggtggaatg tggatttcga atgtatttaa ccggtagtag agatcgctac
     4381 ggagcttgcc ggtttcgagc tgtgtgaaaa cgttctggtt ggtggcagca attatgcgca
     4441 cgtcaacagg aatatcaacg cttgagccaa ttctgcgaac cttacgttct tccagcaccc
     4501 tgagtagctt gctctgcatg gcaggaagca tttcaactat ttcgtccaga aagagggtgc
     4561 ccccgttggc tacctcgaac caaccggctt tgtcctcgtt ggcacctgtg taggaacctt
     4621 ttttatgccc aaaaaattca ctttcaaaaa gcgtttcggg aatggccgag cagtttactg
     4681 cgtagaagta tgagtccttt ctattgctga gataatgaat tccacgggca accagttctt
     4741 tcccagctcc gctttcaccg gtaatcagca ctgaggtgtt ttctgttttg gccaccctac
     4801 ccataaggtt aacaacatgg cttatggcgg gtgatgttcc cagaatctcg ttacccacac
     4861 tttcaatcag ttccttcgaa ataagcgagt aggtctggtt gatttcctta agctggttgc
     4921 tgatggtaat gtagcgcttg gttcgctgga tggccgctcg catgtcgagt aatcggaaag
     4981 gtttcgggaa aaactctact gcacccagcc gcatggcctg tattacagcc tccatgtcgc
     5041 catgtccggt aatcataatt acttcaacgt cggggtattc ctccttaaca cgccggagga
     5101 cctcaaagcc atccatttcg ggaagtttta tatccaggat catgatgtca acttcctgct
     5161 gggtgagcat ctcgaatgct gctgatggcc tatcggagag caataccgtg aaaccatcgt
     5221 tttcgaggaa ttcctttatt tcatcccgaa aaaccttttc gtcgtccagc actaagattc
     5281 ttatttcctt catgcctatc tacattttgg gaagtgagat ttcaaagcgg gtatattcac
     5341 cctcaacgct ttcaacttta atgtcaccta gtaaatcctt aatgaatgca taggataccg
     5401 aaagcccaag cccggttccc ctttcctcgc ttttggttgt aaaaaatggg tcgaatatct
     5461 tgtcgatgat tttgtcggga atgcccatgc cgttatcccg aacgcttagg taaacctgat
     5521 tgttatcctg ccatgtttta atctcaatca gttttgtgta ctggtggttg gcgtgttttt
     5581 tccctttttc gtcaacagca tacttggcgt tggaaagtag gttaaggata acctgctcca
     5641 gcttgtactt gtcggcggta atgctaccga tgttttcgtc aagggtaact tccattttta
     5701 ccccgtggtt ctcgtactgg gtagatacca tgaaaaggga gctgttaacc acatcgttta
     5761 tgttgatttg tgtgaaggta ataggctttt gagctcgtga aaaatagcga atatggtcaa
     5821 taattttttt aatacgctcc acattttcaa aaagtgaggc tattttttcc ttcatgtgct
     5881 cgtcgttgta gcttttatcc tgcaccttca gcaggatgtt gtcgagcccc attgaaatgc
     5941 cccccagggg ttggtttatt tcatgggcaa tgcccgctgc aagcgtacca agggactcaa
     6001 gtttcgactt ttgggcaagc agtagctgtt gtttttcctg tttttttatc tcctgaatca
     6061 tcctgcgctc aagctcgcgg ttcaaatcgg tgatgcgctg ttccgtttca aggcgtttgg
     6121 caatttcctt ctgcttagac cgaaagagta tgattaaaag aacaattagg gtgatgaaaa
     6181 tgatgatgat gagaatcagt attcgcgttt ggtacctggt tttgttaagc tgcaggttct
     6241 gaatctcgga attctgcctc agcagctcat tctccttctc tttctgggaa atctcgtaga
     6301 ggattttaag tttagccagc ttctgtccag tttcctcgaa gtagatggag tcgcgtgttt
     6361 caacccacat tttatagaac tcgagggcat ccctatacct gccctgaccc tcgtttagga
     6421 tggccaggta ctggtaaacg tcggctaccg actgggtgag gttgttttgc cgggcaattg
     6481 ccaaggctct atttaggtat ttagaagcgt tggaaatatc gccgctgctg aggtagcatc
     6541 tcgaaatttt taccagggat tcggtgatag cgggaatatt ttttaacttt tgtgccacgg
     6601 tgagttgctg gttgtagtag tagagggctt gagtaggatt tcctgaatcg aggtaaatat
     6661 cagcaattcc ctgaaggcct tgaatcatgc ctaccgtgtc ggaaattttg ttgctgatgg
     6721 cgtatgaggc tgtaaagtaa aagttggcgc tatcgggttt ttgtagaagg tagtaggtga
     6781 gcgctatttc ctgaagtgca ttggagtagc taagtgaatc aaaattattt atagaaattt
     6841 ctgcagcttg tttaaagttg cttaggcttt ctaccagttc ccccgaattc ttttttacct
     6901 gtcccagctt aaagaaggcg tcagatttga ttttttcctt caaaagcacc tttcggccca
     6961 tcgggttgtc aactattgcc agcaccttgc tgtacatttc agctgatttt ctatagttgc
     7021 cttgtttctg gtaaagttca cccagcagtt ctagcaggtc gatatacagg tttatgttgt
     7081 tgatgttttc gtcagaggca agcccccttt caagggtgga aatggccagc tcgggatttt
     7141 tctcagcatc atacaccctc ccaagcccaa tgagtatctt gtactttgca tcggagttgc
     7201 caagttggtc ataaaagttg agagccctat tatagtagcg tgatgaattt tcatactggc
     7261 caagggtaaa gttcgactcg gctaaaatca ggagagcttc gatttcaaat cgggtatcgc
     7321 ccagcttttt tgccagcact agagcctgta gcccattgtc caatgcctta tctggatttt
     7381 tgacaacatt gtcccttccc tcggatatca ggttggccag aactttttcc tccgatttgg
     7441 aggtaaggct cgaatccggg ctgacctggg cttgaagagt ggggaattgc ataaaaagcc
     7501 agaaggccaa aacaatcgtc gtatgcttcc cgaaccggct catagggtgg ttattgcagc
     7561 actaaagata cccaaattta aaaaaatggc tacttttgga tcgcattttt tgcctgaggg
     7621 acgaattcat cggagtacta tcggtctatt gctaatgttt ttgatcttcc actgttggtg
     7681 aagttttact gtttttttat tcattctgtt gagttatgtg cattacgcac agtaggttta
     7741 aagtgaggca accagaaacg gcttgtttat gtgagcatgc ttcatgtgtt caacggcgtt
     7801 aatacgtttt ttaagaaatg agtaggagta gattgtagga tatttactat ttaaagtgtt
     7861 taatatttgg tgctgatgag tacaattctt tctgtctatg gtaaaaacaa ttaggaatta
     7921 tctttcgctg gttaagttta gccacactgt atttgcattg ccgtttgcag taattggatt
     7981 ttttttggcc tatcggcaaa cccctcagaa tgttgatgtt tggttgtttg tgctggtggt
     8041 gctctgtatg gtttttgccc gtaatgccgc catgagcttt aaccggctag tcgaccgtcg
     8101 tttcgacgcc attaatccaa ggacgcaaaa cagagaaatt cctcgcggca taattacctc
     8161 cagggctaca gccgtgtttg ttacggtgaa tgtgttgctc ttcattgcaa ccacctactt
     8221 catcaaccgc acggtgtttt tcctttcgcc aatagctttg gcaatcgtgc ttctgtacag
     8281 ctacaccaag cgatttacca tgttttgcca ttttgtgctt ggtcttggat tggccctggc
     8341 gcctataggt gcctacttgt cagttacccc caggttcgag ctgttgccgt tactttactc
     8401 ggttatcgtg ctattctggg tatccggttt tgatatcatg tatgccctgc aggatgaaga
     8461 ttttgataag cgggaaaatc taaagtcgtt accggcagca ctgggcgtta aaaaatctct
     8521 tttggtggcc aaccttctac attttgttgc aggagctgtc gtagttctca ttggtatcct
     8581 atatcactcc gatgtatggt actgggtggg tgcagccctt tttatcgccc tgctgatata
     8641 ccagcatcta atagttaaac ctaccgatat atcgagggtt aacatggctt tcgctaccac
     8701 aaacggtctg gcaagcatta tttatgctac gtttaatgtt atcagtattt ttttctaact
     8761 tgcacaagct agttttaaag gacattttca actaaatttt actcaaaatc acagaagtat
     8821 gatgaaaaaa gtatttttac tcgcagctac agcagcaagt attcttgcct gctcatgcgg
     8881 aacaaagagc agttctgaca accagcaggc aaaagccgat tcaacagcag cgtcaattgt
     8941 tgaggttagc attgattctc tcatctcaaa cccacaggca tttgccaacc aaaccgtaaa
     9001 gtttactgcc aaggttgatc atgtttgcat gcatagcggc caaaagctta cgctcatggg
     9061 cagcgattcg ggcagcacct tcaaggtaat ggcaaccgat gccgttcccc agttcgatca
     9121 gaatctcaac ggaaccactg ttgaggttgt tggcgtggtg cagggtgcag cacctgaaga
     9181 tgtggaatca tgcagcgaag aggaaggtgg acagcagaaa gcaaaaaaac tcgactatgc
     9241 agttttctgc caaaaagtaa gggccctcta gcattctgcc ttaattaaac tatactccgg
     9301 tggcaatcct tcacgcctta tggtggagga ggatacctgc cggttttttt ataccaggga
     9361 aaatatataa gttaatcgtt catgaacatg cgcaagctgc tcagggtgct gcaccgtgat
     9421 tttggttact ttttcacggg aatgactctt atatactgtg taaccggaat tttgctcaac
     9481 catacccgcg actggaaccc ctactactcc attgatattc gtaaggtgga gttgaatctc
     9541 ccactcgaca catcattaat taacaaggag ttgctgactt ctaagttgga acctattggt
     9601 gtacaggggc taaggtcgtt ctactaccct aaacccggaa cagtcaagct atttgtttcc
     9661 gatggaacgg ttacttcaga tctagggagc ggtagcgctc tggtggaaag aagctaccgc
     9721 aggcctgttc tctaccagct taacatgctg cataaaaatc tacccaaatc atggtggact
     9781 tggttcagcg atatttttgc catagccctg atggcaatta ctatatcggg gcttttcctg
     9841 gtaaaaggga agaatggcat tacccatcgt ggtgttattc tcattattgc tggcttagca
     9901 cttccccttg tggccataat tctcctctaa acatttttag ctgggttgat aggaatattt
     9961 agctaatgtt gactaaattt gcttctacaa gtaagcatca tatcggcatt cagcataatg
    10021 aattattgcg accttcattt taaattttct gccaagtacg tattcctgaa catcaagaac
    10081 agggataact ttttctaatg ccgtcatttg catagccatc ccccggctat gcagcactgt
    10141 ttaagttatc cctaagtttt aattttttta cgctaaaaaa ttatcgatat ggaactacca
    10201 tttgcagaat cgtacaaaat caagatggtt gagcctatac accggagcac ccgaagcgaa
    10261 agggagcgtt ggattaagga ggccaagtac aacatgttta acctgaagag cgagcaggtt
    10321 tttattgatt tgctcaccga ttccggaacc ggtgccatga gcgacaggca gtggagtgcg
    10381 ctcatgatgg gtgacgagag ctatgcggga gcttcctcat actacaacct taaaagcgcc
    10441 atccagaata ttctgggatt tgagcacttt ttgccaaccc atcaggggag agctgccgag
    10501 aatgtccttt tctccgtgat ggtgaaagag ggcgactaca ttcctggcaa ctcgcagttt
    10561 gataccacca aggggcatat tgaatttaga aaagccgagg cggttgactg caccattgat
    10621 gaggcagaga atacagagat ttaccaccct tttaaaggaa atgttgaccc ccagaaactt
    10681 gatgacttta tccgtaccca cgccgacagg gttccatttg tcattatcac cattaccaat
    10741 aactcagcag gaggacagcc agtatcaatg caaaacctga aggatgcccg aaaaatttgc
    10801 gataagtacg gtaagccgct ggtgattgat tcggccagat ttgctgagaa tgcctacttt
    10861 atcaaggttc gtgaaaaggg ttatgccgat aaaaccatta aggagattgt gcgcgaaatg
    10921 ttctcgtatg ccgatgctat gaccatgagc agcaagaagg atgccattgt taatataggc
    10981 ggatttattg caatgcgcag caaggagctt tggcagcaat gctcaacctt caacattatg
    11041 ttcgagggat ttgttaccta cgggggcatg tcggggcgtg atatgaatgc cctggcggtt
    11101 ggtcttgatg aaggaacgga gttcgactac ctcgaaacac gcattaaaca ggtggagtac
    11161 cttggtaaca ggctgaagga ttttggtatt cctttccagt tccctgctgg tggacatgcc
    11221 atttttgttg atgccaacaa ggttctggct aatgttccca gggagcagtt tcgtgcccaa
    11281 acgctggctg ttgagctcta cctcgaggca ggcattcggg gtgttgagat tggtgccctg
    11341 atgtcggata gagacccggt tacccgcgag gtacgaatgc ccaagctaga acttctcagg
    11401 ttggctatcc cccgcagggt ttacaccaat aaccatatag acgtggtggc cgttgccctt
    11461 aaaaatgtat tcgatcgtag aatggacatt aaaaatgggc tacaaattac atgggaagcc
    11521 ccaattatgc gccattttac cgttcagctc gatagagcac gataatcttt ttcaactctt
    11581 ttgccattgg cttgttctgt agtggatcag gccaatggtt ttttatttat catttacccc
    11641 aaaaatagcc tacttttgca attcatttct atgcaatgag ggattttaca accggtagcg
    11701 aaacccaaca tattttaagg tttgcaacac ccatgctgat aggcgatatt tttcagcagc
    11761 tctacaacgt ggttgatagc attattgttg gtcgctttat tggcgataag gctttggcgg
    11821 ctgttggagc atcgtttccg gtaatctttg ttattattgc ccttgtgctt ggcattggca
    11881 taggttctac ggttgtcatc tcgcagtact ttggtgcgcg taacttcgat atggtgaagc
    11941 gtgcagctaa caccatctat atttttcttc tgatttcggg cgtggtggtt accgccattg
    12001 ggctgatggc aagcggagcc atttttagga tgcttcacct gcctgaagaa ctcatgaagc
    12061 cagctaggac ctacctcaac atttttatgc tgggcatggt ggttatgttt gccttcaacg
    12121 ctatagccag cattcttagg ggaattggcg attctaaaac tcctttgtat tttcttctta
    12181 tttcaacgct taccaatgtt ggattggtgc tactttttgt ggtaaaattt cgatggggca
    12241 tagccggtgc tgcatgggca actgtcattt ctcaggttgt ggcgtttatt atagccttaa
    12301 tctacttaaa ccacagccag cacatgctta gcataaacct acgaaccatg aagttcgacc
    12361 gccaaatttt caggcagatt gttaaaattg ggttacccac cggaatacag caaactatgg
    12421 tagcccttgg ctcaatggcg atgatgggaa ttgttaattc ttttggaacc accgttattg
    12481 cggcttttac tgttgctagc cgtttggatg cactaattgc aacccctata atggattttg
    12541 ccgccgctct ttcgggattt gtggggcaga acattggtgc caacaagatt gacagggtaa
    12601 aaagggggct acggtcaacc ctg
//
LOCUS       DAOPHX010000074        12564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_5932_length_12564_cov_5.483652, whole genome shotgun sequence.
ACCESSION   DAOPHX010000074 DAOPHX010000000
VERSION     DAOPHX010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 12564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12564
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5932_length_12564_cov_5.483652"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(618..1517)
                     /locus_tag="PK502_06710"
     CDS             complement(618..1517)
                     /locus_tag="PK502_06710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPH00543.1"
                     /translation="MDKRNGTETSAFGTKGRINHDSSKFYSSKLYSEINNNFIGDKAE
                     NQIPNEYENRIILGSAENMKELPNNSVHLMITSPPYNVSKEYDSDLSLSEYLQLLQNS
                     FKETYRVLVYGGRACINIANIGRKPYIPLSDYISRMMIDIGFNMRGEIIWNKASSSSP
                     STAWGSWQSAANPTLRDVHEYILIFSKGDYKREKGDKTDTISKEQFLEWTKSIWTMNA
                     ESAKKIGHPAPFPEELPYRLIQLFSFTDDVVLDPFIGSGTTAISALKSKRRYVGYEIS
                     EEYVNLAERRISPIKQQSVINFR"
     gene            complement(1520..2380)
                     /locus_tag="PK502_06715"
     CDS             complement(1520..2380)
                     /locus_tag="PK502_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00544.1"
                     /translation="MILWNNDIEIQFFEEALKNFASPEKLFYKLNTGYFAYIPKGTEN
                     EGHTLQSRNSLIGQFTEKWCKSILEPIAGKFGLYAINGVVCEEIGLSRQSSADLALCT
                     SNKIIQRPENIKLIFEIKMSIVSNYKYTEPNRIEFISDYKHHKGNPSLLRSDSMLKAI
                     GKSISIRVSGNSSCKIPIVTIGNSPISKNYVDKIDFLKSSGVIQGFWSLNPNPCNSEF
                     IKETPQKGFQTFKNQKMITESCKELLESDMLYFSSMVSMPKLGEIIRLANKEKTDAAK
                     AEKFLTLIRG"
     gene            2545..2967
                     /locus_tag="PK502_06720"
     CDS             2545..2967
                     /locus_tag="PK502_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPH00545.1"
                     /translation="MEKVAIQAEWLEGLAFETEVNGHKLIVDADENAGGSNRGPRPKP
                     LMLVALAGCTGMDVISILKKMRVDVKRFSVGVEAMQTDEHPRHYVGMNIIYEFWGKDL
                     PPESINKAINLSQEKYCGVSASYRKAMPITFEVKIHEE"
     gene            2981..3190
                     /locus_tag="PK502_06725"
     CDS             2981..3190
                     /locus_tag="PK502_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00546.1"
                     /translation="MNITGNTLIEDLVEQLPESVSFLREKGIICIVCGEAVWGTLAEV
                     AQQKGLSDEAIEATIEELNQLKKSK"
     gene            3552..4571
                     /gene="asnA"
                     /locus_tag="PK502_06730"
     CDS             3552..4571
                     /gene="asnA"
                     /locus_tag="PK502_06730"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HPH00547.1"
                     /translation="METPAKLYQNVLDARQTEDAIALIHDFFPGKLAAVLNLRRVTAP
                     LIVPSGMGINDDLNGVEQPVRFSAKSLGNREVEVVQSLAKWKRITLARLKVEPGKGIY
                     TNMNALRPDETLDATHSIYVDQWDWEKCIADAQRNLEYFRRTVEQIYSAMKDLESHIF
                     EHFPQLAPVLPDKIHFIHTSELEARFPNLSHRQREYESCRELGAVCIEGIGYPLSNGH
                     PADGRAPDYDDWSTLRGDGKRGLNGDILVWHPTLQNSLELSSMGIRVNSEALKLQLAM
                     QDCDNRRGLWYHRQLLEDKLPQSIGGGIGQSRLCMFLLRKSHIRDVQASVWPDECCAS
                     NNAMM"
     gene            4771..5556
                     /gene="truA"
                     /locus_tag="PK502_06735"
     CDS             4771..5556
                     /gene="truA"
                     /locus_tag="PK502_06735"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPH00548.1"
                     /translation="MPRFKLTIEYDGSHYRGWQFQREMPTVMGKLMDAIKEVYKVHEY
                     ELYGSGRTDGGVHALAQIAHLDIESDLPPHMAKSRLNEELPSSINILNIEKVSPRFHA
                     RYSAIARSYVYHISKRRTAFGKDYTWWVKDELNVKDMADATTLFTGMKDFRSFGMAEK
                     DDSSTLVKLQTVKLFEVDDSILVHIVGSHFLWKMVRRVVGTLVEVGRGSITGDDIQRF
                     FSEYSEQPARFTAPPSGLYLERVYYPGDKIADEPEWLMNISHH"
     gene            complement(5720..7261)
                     /locus_tag="PK502_06740"
     CDS             complement(5720..7261)
                     /locus_tag="PK502_06740"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPH00549.1"
                     /translation="MRSLEKLYSELEARNQAAEMAGGQKRIDKQHEGGKKTARERIVE
                     LLDPGTFTEIDKFVTHRTVEFGMADKHIPGDGIVSGYGKIDGRRVYVYAYDFTVFGGT
                     LSRANADKIIKLQQLALKMGVPVIGLNDSGGARIQEGVQSLAGYAEIFYNNVRSSGVI
                     PQITCIMGPCAGGAVYSPALTDFIVMVKDTSYMFVTGPDVIKAVTHEDVTKDELGGAV
                     THNSKSGVAHFIADNDEQALMLVRELMSFLPINNMEEPPIVPTTDDSTREDELLQTLI
                     PADPNKPYDIKDLILSVVDDRNFLEVQALFAKNIVVGFARMAGHTVGIVANQPAHLAG
                     VLDIDASVKAARFVRFCDSFNIPLVTFVDVPGFLPGVNQESNGIIKHGAKLLYAYSEA
                     TVPKIAVITRKAYGGAYCVMSSKQVGADVNFAYPMAEIAVMGPDGAVNILYRDRIDNE
                     EVRLQLIEEYRETFANPYRAAELGYVDEVIYPKQTRYKLIQALEMTRSKSQPTPPRKH
                     GNIAL"
     gene            complement(7342..7851)
                     /locus_tag="PK502_06745"
     CDS             complement(7342..7851)
                     /locus_tag="PK502_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HPH00550.1"
                     /translation="MGLEVKIGDRVADVKLVKREGQQVTIEVDGKLYEVDIAQVENGV
                     YSILHNGKSYNIEIIPGDGPKSYFVNTFYNSHEVEIIDAHSRYMMSRNLSSENAADRV
                     ISTPMPGRVVKIPVTEGEQVSKGATVIVISAMKMESEYKSPVDGTIRKIHVAEGDNIA
                     ANQPLIEIE"
     gene            complement(7862..9376)
                     /gene="accC"
                     /locus_tag="PK502_06750"
     CDS             complement(7862..9376)
                     /gene="accC"
                     /locus_tag="PK502_06750"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HPH00551.1"
                     /translation="MIKKVLIANRGEIAVRVIRTCREMGILTVAVYSDADRKALHVRY
                     ADEAYYIGPSPSRESYLNGDRIIEVALRSGANAVHPGYGFLSENADFARKCAEAGLIF
                     IGPDPWAMTQMGDKITARQTMIKAGVPVVPGTQQKVDDIQAVRRIVDEIGLPIIIKAS
                     AGGGGKGMRLVRQPSELVSALKMAQSEALSSFGDDTVYIEKYVEAPHHIEFQILADKF
                     GNTVHLFERECSIQRRHQKVVEETPSPFMTPELREKMGQAAIAAAKAVNYTGAGTIEF
                     LVDAERNFYFLEMNTRLQVEHPITERVTGVDLVKEQIRIASGMPLSFSQNQIHQHGHA
                     IECRVSAEDPFNNFMPSPGLVRHISEPNGLGIRTDGYVYEGYEIPIHYDPMISKLISW
                     GRTREEAVDRMKRALFEYKITGVKTSLRFLSRIMENKDFRNGVYNTHFVPDHFDELIG
                     GEATEKPIYEDMAVIAAYFDFTSKMERVAMNDFSIQHKPDSWKVYGINRNLMRI"
     gene            complement(9752..10480)
                     /locus_tag="PK502_06755"
     CDS             complement(9752..10480)
                     /locus_tag="PK502_06755"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HPH00552.1"
                     /translation="MFYKKFTYLNLLLLFILGCSTNEIIDPGDVETRRVEISKSYRSI
                     EVENTFEITLVQDSLSYALVTCGEKLQQDVSIMVKDSILFLKNNLNLHFLKGYDKVKV
                     ELHLNEIPITNIRKPSKIQTAGVFRTPTFYLVDWNSFTDCNVNLEVDNLILSISGSNF
                     GSYHVEGKAISASLFCEGTALFDLANMSIDSCCVSHDGLNDVFLHVNRYLSATFTSKG
                     NVYYSGEPICLVSGDGSGRLIKVN"
     gene            complement(10521..11657)
                     /locus_tag="PK502_06760"
     CDS             complement(10521..11657)
                     /locus_tag="PK502_06760"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HPH00553.1"
                     /translation="MGWFGKGLRLKVAFSAMGLLAVITGADAQDAPIRDRLFFGVEPR
                     WGFVMPHHDYMAYFLESNVNSFQLNVGLNTTGYKDWHKSFNYPQLGGGFFYSGLGNDR
                     IYGKMLAAYVFIDRQFLPRKNRFNIGNRAAFGMSYLTKQFDLHSNPYNMVIGTPFNVF
                     FQYDLTLYYRLNSNLNLRWALGFSHASNGNLREPNKGFNLITSGFGLQYSISDDRLLV
                     TQAPGYHSDSTRTYVSIGVLGSAKSVSRENTNLYPVYGMSVEWLRRLSKTSLAGVELA
                     AYKDKAIKYTYQAKDDSTYRSSDSYAVTLNPAYIMQLGRTMFVFQPGIYLKRGYKPDG
                     YITNKVGMRFQLSQNLYASWAIKAHWAAKADFVEFGIRYRIIAD"
     gene            complement(11669..12283)
                     /locus_tag="PK502_06765"
     CDS             complement(11669..12283)
                     /locus_tag="PK502_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPH00554.1"
                     /translation="MKKPGKLCVILIISACPAVVFSQGFKAGFTLGIAATQVDGDTYG
                     GFDKAGPVAGVWVGRNLGNSWLGMMELRYAQKGSYAKTTVNEFNGYYRMRLHYFELPV
                     MGGYRFVNGFQTLGGISLGYLAKAEEMTELGPFPEEDISAFNQFELAMLCGVEYNYSE
                     HWAFGAFFSYSIFPIRPYKGNITYRMNRGQYNQVVELLVRYKIQ"
BASE COUNT         3476 a         2971 c         2734 g         3383 t
ORIGIN      
        1 atgagtccta gaaggttatc acctaacaac gaataaaagg cttcgctacg ctttgcctat
       61 attgccttcg gcgacttctt ttatccgtaa tccgttgtgc ggtcatttca taacgaccac
      121 gtcttcaagg attatgtgaa taaaaacact gcaccctatg gtttcaaatc cgtggtataa
      181 gttagatttc ccccgctacc ccgcgagccc ctcgctcggc gtagttttgt gcattaggat
      241 ttgcgcgtag gggaactatg tcgtggctaa aggacagctt acaactgtct tgttgtaagg
      301 caaagtttgt ttttcgttgg tagtagtttt cggtctggag accggaactt tagcaagtat
      361 ttgacacggt caggaacctc cacttcagta ctacgaagta acagacttcg ccgagcgagg
      421 gccagggatt catgctaagc atggggctag ccaaaatgta cgttgatgcg ctaacgggaa
      481 aacctgttcc cgactacttt ggccgcttaa gcctcgaagg cgacggcctg cccgagaaag
      541 cattcaagta aagctggata ctccacttca ctcaaaagcc ctgccaacct ttgctggcag
      601 ggttttttcc ataaaaccta cctgaaattt ataaccgatt gctgcttaat aggggaaatt
      661 ctcctttcgg ccagattcac atattcttcc gatatttcat acccaacata tctccttttc
      721 gattttaagg cgctaatagc ggtagtacca ctcccgataa atgggtccaa aacaacatca
      781 tcggtgaaag aaaatagctg gattaatcta tagggtaatt cttcaggaaa tggggcaggg
      841 tgtcctattt tctttgcgct ttcagcattc attgtccaaa ttgactttgt ccactccagg
      901 aactgctcct tggatatggt gtccgtcttg tccccttttt ctcttttgta gtctcccttt
      961 gaaaaaatta atatgtattc gtgtacatct cttaatgtag gattggcggc gctctgccaa
     1021 ctcccccatg ctgtagaggg gctagaactt gaagctttgt tccatattat ttcgccgcgc
     1081 atgttaaatc ctatatcaat catcattctc gaaatgtagt cagagagagg aatatatggc
     1141 ttacgcccaa tatttgcaat atttatacat gctctaccac catataccag cacccgatag
     1201 gtttccttaa atgaattttg caaaagttgt aagtattcgc ttaacgataa atcgctatcg
     1261 tattcctttg aaacattata gggaggcgag gttatcatta aatgaaccga attattaggc
     1321 agttccttca tattttcagc tgaacccagt ataatccggt tttcgtattc gttggggatt
     1381 tgattttcag ccttatcacc tataaaatta ttatttatct cggagtatag cttcgagcta
     1441 tagaactttg aactgtcatg attaattcta cccttagttc caaaggcgct ggtttctgtc
     1501 ccatttcttt tatccatagc tatccccgga ttaatgttaa aaatttttcg gctttcgcag
     1561 catctgtctt ctctttgtta gccaatctaa taatttcacc aagttttggc atagaaacca
     1621 ttgatgaaaa atagagcata tcgctttcta acaattcctt acaactttct gtaatcattt
     1681 tctggttttt gaatgtttga aagccttttt gcggagtttc tttgataaat tcggaattgc
     1741 agggattagg gtttagcgac caaaaacctt gaataacacc tgaagatttt aaaaaatcaa
     1801 ttttatcaac ataatttttg ctaattgggc tattgccaat agtaacaatg ggaattttac
     1861 acgaggaatt tcctgaaacc cgaatgctaa tagattttcc aatagctttg agcatagaat
     1921 cggaacggag caatgaagga ttacccttat gatgcttgta gtcgctaata aattcaattc
     1981 tgttcggctc agtatattta tagtttgaaa cgatactcat cttaatttca aaaataagct
     2041 tgatgttttc gggacgttga atgattttat tcgaagtgca aagcgccaga tcggcagatg
     2101 attgcctcga taggccaatt tcttcacata caacgccatt aatggcgtac agtccaaatt
     2161 taccagctat gggttcaagg atagatttac accatttctc ggtaaactgc ccaatcagcg
     2221 aattcctact ttgcagggtg tgcccctcat tctccgtacc tttaggaata tatgcaaaat
     2281 aacctgtatt caacttgtaa aataactttt ccggagaggc aaaattcttc agtgcttcct
     2341 caaaaaattg aatttcaata tcgttattcc acagaatcat tcgcaacaca tttttacaaa
     2401 tttagcaatt atcaagcata tagaagttga ttacatgatt tcttttagta ccccgctggt
     2461 gctttaccct ttcatacttg atctgtaaaa atttttttga actttacccc aaaaattcgg
     2521 ttcttacata tcaaaactaa cactatggag aaagtagcta ttcaggccga atggttggaa
     2581 ggcttggcct ttgaaacaga ggtaaacggc cacaagctga ttgttgatgc cgacgaaaat
     2641 gccgggggaa gcaaccgcgg cccccgccct aaaccgctta tgctggttgc cctggccggc
     2701 tgcacgggaa tggatgtaat ttctatcctt aagaaaatga gggttgacgt aaaacggttc
     2761 agcgttggcg ttgaggccat gcaaaccgat gagcacccca ggcactatgt tgggatgaac
     2821 ataatctacg agttctgggg aaaggatctt ccgcccgaaa gcatcaacaa ggccatcaac
     2881 ctgtcgcagg aaaagtactg tggggttagc gcctcatacc gcaaggctat gccaattact
     2941 ttcgaggtaa agattcacga agagtaacag gagaataagg atgaacataa ccggcaatac
     3001 cctgattgag gacctggtgg agcagctacc cgaatcggtt tcgttcctta gggaaaaagg
     3061 aattatctgc atagtttgcg gagaggctgt atggggaaca cttgctgagg tagcccaaca
     3121 aaagggactt tccgacgagg ctattgaagc aactatcgag gagctaaacc agctaaaaaa
     3181 atcaaagtaa attttgagaa tttaaaggaa aatcctacct tgcccttgaa aatttggatg
     3241 aaatgattca catattccgc aataattgca attacaattt caacaataat ggctggttgt
     3301 tgaaagggga ttgctattgc tgaaataaac attgattacc cgcaaaaggc tttcccaaaa
     3361 agggagagcc ttttttgatt gcagtaaccc aactagaagt aacgccatgc aaaccccgct
     3421 actaaacgga aataataata accgcccacc tccctccggg tcaggatcat gctgtatttg
     3481 cccttcccgg aaaaaccgtg tgaagggttt tctttttatg caagttcctc aacctaaaaa
     3541 aaataaatta catggaaaca ccagctaaat tataccaaaa cgttctggat gcccgccaga
     3601 cagaagatgc aatcgccctc atccacgatt tctttcccgg gaaattggca gcagtgctaa
     3661 acctacggcg tgttaccgca ccgctgatag taccctcggg tatgggtata aacgacgacc
     3721 tcaacggggt tgaacaacct gtcaggttca gcgccaaatc gctgggcaac agggaggttg
     3781 aagttgtgca gtcgctggcc aagtggaaaa ggataaccct tgccagactt aaagtagagc
     3841 caggaaaggg tatctacacc aacatgaatg cactccgacc tgacgaaacg ctcgatgcca
     3901 cacattcaat ttacgttgac cagtgggatt gggaaaaatg tatagctgat gctcaacgca
     3961 acctggagta cttcagaaga acggttgaac aaatttactc cgccatgaaa gatttggaat
     4021 ctcatatctt tgagcacttt ccccaactag cgccagtttt gcccgataaa atacacttca
     4081 tccacaccag cgagctggag gcaaggtttc caaacctttc gcaccgacaa cgcgagtatg
     4141 aaagctgcag ggaacttgga gctgtatgta tcgagggaat tggctatccc ctatcgaatg
     4201 gccatcctgc agatggtcgg gcacccgact acgacgactg gtcaaccctc cgaggtgatg
     4261 gtaaaagagg cctcaacggc gatatcctgg tttggcatcc cacccttcaa aattcactgg
     4321 agctctcgtc tatgggaatt agggttaaca gcgaggcgct taagctgcag ctggcgatgc
     4381 aggattgcga taatcgtcgc gggctttggt accaccgcca actactcgag gacaagctgc
     4441 cacaaagcat tgggggagga ataggccaat cgcggctttg catgtttttg ctacgaaaat
     4501 cacacatacg ggatgttcag gcctccgttt ggcccgacga atgctgtgct agtaacaacg
     4561 cgatgatgta gaaaccccat attaccttaa aggtgtgaac cggtaggtat tggaatacta
     4621 ctgaaaatag cctcaaatat attataatca acttgctgaa aaccaccctg catggctggc
     4681 agtaacctat tggcagctgc acatggctca cctttagcac cctaaacttc tacagctggg
     4741 aataaagctg tatatttgta acaaaaaatt atgccccggt tcaagctgac aattgagtat
     4801 gatgggtccc attaccgtgg atggcagttt caacgcgaga tgcctacggt tatgggtaag
     4861 ctgatggatg ccattaagga ggtttacaag gtacacgaat acgagctgta cggttcggga
     4921 cggactgatg gaggcgttca cgctctggct cagatagcac acctggatat tgaatctgat
     4981 cttccgcctc acatggcaaa gagcaggctc aatgaggagc tgccctccag catcaacata
     5041 cttaacatcg aaaaggttag cccccgtttc cacgcccgtt acagcgctat tgcccgtagc
     5101 tatgtttatc acatatctaa aagacgtact gcttttggta aggactacac ctggtgggta
     5161 aaggatgagc tcaacgtaaa ggatatggcc gatgccacaa cccttttcac ggggatgaag
     5221 gacttccgat catttggaat ggcagaaaag gacgacagct caaccctggt taagctgcaa
     5281 acggtcaaat tatttgaggt tgacgattcc atactggtgc atatagttgg ctcgcacttc
     5341 ctttggaaaa tggttcgtcg agttgtgggg acactggttg aggttggaag gggaagcatc
     5401 accggtgatg atattcagcg atttttttct gagtactccg aacaacctgc ccggttcacc
     5461 gcacctccat cgggactcta cctggaaagg gtttactacc cgggcgataa aattgccgat
     5521 gagcccgaat ggcttatgaa tattagtcac cactagcctt tactgtgctg aaaaaaagta
     5581 aacgcctgac ttaacaagtt caggcgatta ttttttaaaa gagaagtggc tacgttactc
     5641 ttgtaaaaag atgaacgctc gcatctcaac atttaaagat tcacatataa agcccttatt
     5701 aagaatggga taagccttcc taaagagcaa tatttccatg cttgcgtggc ggagtaggct
     5761 gactcttact gcgggtcatc tccagtgcct gtatcagctt gtaccgggtt tgcttaggat
     5821 agataacctc gtcaacatat cccagctcag ctgccctgta ggggttggca aatgtttcac
     5881 ggtactcctc aatcagctgt aggcgaacct cctcgttatc tatccggtca cggtagagaa
     5941 tattaacagc cccatccggc cccataacgg caatttcggc catggggtat gcaaagttaa
     6001 catcagcacc aacctgcttg ctcgacatca cgcagtaagc accaccataa gccttacggg
     6061 taataacggc tattttggga acagttgcct cggagtaggc atagagcaac ttcgctccat
     6121 gcttgataat gccgttagac tcctggttca ctcccggtag aaatccgggg acatccacaa
     6181 aggtaaccag cggaatgttg aaggaatcac agaagcggac aaatcgggca gctttaaccg
     6241 aagcatcaat atcgagaacc cccgccaggt gggcaggctg gttggctaca atgcccacgg
     6301 tgtgtccggc catccgggca aaacccacca caatattctt tgcaaaaaga gcctgaacct
     6361 ccaggaagtt tctatcgtcc actaccgaca ggatcaaatc ctttatatcg tagggtttat
     6421 tcgggtcggc gggaatcagg gtttgcagta gctcatcctc gcgggtagaa tcgtcggttg
     6481 ttggcacaat tggaggctcc tccatgttat ttatgggcag aaaactcatc agctcccgca
     6541 caagcattag ggcctgttcg tcgttgtcgg caataaagtg ggccaccccg cttttggagt
     6601 tgtgggtaac cgcaccgccc agttcgtcct tggtaacatc ctcgtgggtt acagctttaa
     6661 tgacgtcggg gccggttaca aacatgtagc tggtgtcctt taccatcacg atgaaatcgg
     6721 ttagagcggg tgagtaaaca gctccgcctg cacacggccc catgatgcag gttatttgag
     6781 gaattacccc ggagctcctc acgttattgt agaatatttc agcatatccc gcaagacttt
     6841 gcacgccttc ctgtatgcgg gcgcctccgg aatcgttgag cccaatgacc ggaacaccca
     6901 tttttaaggc cagctgctgg agcttgataa tcttgtcggc attggcacgg ctcagcgttc
     6961 ctccaaatac cgtgaagtcg taggcataaa cgtaaacccg tcgcccgtcg attttaccgt
     7021 atccggatac gattccatca ccgggtatat gcttatcggc catgccgaat tctactgtcc
     7081 gatgggttac aaacttgtct atttcggtga acgttcccgg atcaagcagc tcaacaatac
     7141 gctcgcgggc ggttttcttt ccaccctcgt gctgcttatc aatacgtttt tgaccaccgg
     7201 ccatttcagc cgcctggtta cgagcctcca gctcgctgta aagtttttct aaagacctca
     7261 taaaaagatg gttaaatttg gcaggtagta gcttgaccta ccgcaaaatg aatcataaag
     7321 aataagtagc ccttaataga gctactcaat ctcgataagg ggttgatttg cagcgatatt
     7381 atcaccctcg gcaacatgaa tctttcggat agttccatcc acggggctct tatactcgct
     7441 ctccattttc atggccgaaa ttacgattac cgttgctcct tttgacacct gctcgccttc
     7501 ggttacaggg attttaacca ccctaccggg cattggtgtt gaaataaccc tatctgctgc
     7561 attttcagac gataggtttc ggctcatcat gtaacggctg tgggcatcta taatctcaac
     7621 ctcatgcgag ttgtagaagg tattcacaaa gtaactttta ggaccatcgc cagggataat
     7681 ttcaatgttg tacgatttgc cgttgtgcaa aatggagtaa actccgtttt ccacctgggc
     7741 aatatccacc tcgtagagtt tcccgtcaac ctcaatggta acctgctggc cttcccgttt
     7801 aacaagcttt acgtcggcaa cccgatcgcc aatttttacc tcaagtccca tcctctgaaa
     7861 gttatatgcg catcaagttt ctattaatac cgtaaacctt ccagctgtcg ggcttatgct
     7921 gaatggaaaa gtcgttcatg gcaacacgct ccatcttgga ggtaaaatcg aagtaggcag
     7981 caataacggc catatcctca tatatgggtt tttctgtagc ttcaccgcca atcaactcgt
     8041 caaaatgatc gggtacaaag tgggtgttgt acaccccatt gcggaaatcc ttattttcca
     8101 ttattctgga aaggaagcga agcgaggtct ttacaccggt aatcttatac tcaaaaagtg
     8161 cccgcttcat gcggtcaacc gcctcctcgc gagttctacc ccacgaaatc agcttgctaa
     8221 tcataggatc atagtgaatg gggatctcgt atccctcgta aacataacca tcggttctaa
     8281 ttcccagccc gttgggttca cttatatgcc gcactagtcc gggtgatggc atgaagttat
     8341 tgaagggatc ttcagcagaa acccgacatt caatcgcatg gccatgctga tggatttggt
     8401 tctggctaaa cgaaagcggc atacccgatg caattcggat ttgctccttt accaggtcaa
     8461 cccccgtaac cctttcggtg atgggatgtt caacctgcaa acgggtattc atctccagga
     8521 agtaaaagtt gcgttcggca tcaaccagga actcaatagt ccctgcgccc gtataattca
     8581 cggctttggc tgctgctata gcggcctgcc ccattttctc acgcagctca ggagtcataa
     8641 aaggcgatgg ggtttcctcc acaaccttct ggtgcctgcg ctggatggaa cactcccttt
     8701 cgaacagatg aaccgtattg ccaaacttat cagctagtat ttgaaactca atatggtggg
     8761 gtgcttcaac gtacttctct atgtaaaccg tatcgtcgcc aaacgaggaa agcgcctccg
     8821 attgtgccat tttcagagcc gacaccagct cggacggttg gcgtacaagc ctcattccct
     8881 taccacctcc tccggcagaa gccttaatga tgatgggcag cccaatttca tcaacaatac
     8941 gccgaactgc ctgaatgtcg tcaacctttt gctgggttcc gggaacaacg ggaacgccgg
     9001 cttttatcat ggtttggcgg gcggtaattt tatctcccat ttgggtcatg gcccagggat
     9061 cggggccaat aaaaataaga ccggcttctg cgcacttacg tgcaaagtct gcattctcgg
     9121 acaaaaaacc atagcccgga tgaacggcat tggcgccgct acgtagcgcc acttcaataa
     9181 tcctgtcacc attcaggtag ctttctcttg agggcgaagg tcctatgtag taggcctcgt
     9241 cggcgtagcg aacatgcaag gctttacggt cggcatcgga gtaaacggcc acagtaagaa
     9301 tgcccatttc ccggcatgtt cttataaccc ggacggcaat ttcgccccgg ttagcaatta
     9361 gcactttttt aatcataagt cccagatttg aatccgctgc aaatgtaaac acttttatat
     9421 gttatataac atattttatt aattttttat cgaaaaaaat gctataattt ttttaaaatt
     9481 ttaacgcaat cgttttaaat ataaggaaag atggccattg cagggataaa aaaattataa
     9541 acatggtgcc aggatacgag aaaatcatca atcaaagaaa tttttctcca tataatgtta
     9601 tctatttaat tactaactgt ttaaaatata ataatttacc agaagcatca aattatgttt
     9661 agtttttatt gaattataat gaggtagaaa cagtcatgct taaattacct ggggaaaatc
     9721 gtagccatga gcaagaacat ccaccaatgt gctagtttac cttaatcagc ctgccacttc
     9781 catctcctga caccaaacaa attggctcgc ccgaatagta cacgtttcct ttcgacgtaa
     9841 aggttgcgct taaataccta ttaacatgta ggaaaacatc attcaacccg tcatgcgaaa
     9901 cacagcaaga gtctatgctc atgttcgcca ggtcgaacag ggctgttccc tcgcagaaaa
     9961 gcgaggcgct aatggctttc ccctccacat ggtagctgcc aaagttagaa cccgaaatac
    10021 tcagtatgag gttgtccacc tcaaggttga cgttgcaatc ggtaaaacta ttccagtcaa
    10081 caaggtagaa cgttggtgta cgaaaaacac cagccgtttg aattttgctg ggttttcgaa
    10141 tgttagtaat aggaatttcg ttgaggtgaa gttcaacttt aaccttatcg tatcctttaa
    10201 ggaagtgcag gttaagatta ttcttcagga aaagaatgct atcctttacc ataatgctca
    10261 catcctgctg aagtttttcg ccacaggtta ccagagcata ggaaagcgaa tcctgaacaa
    10321 gggttatttc aaaggtattc tccacttcaa tggacctgta gcttttcgaa atttcaacac
    10381 gccgggtttc aacatcgccc gggtcaataa tctcattcgt actgcaaccc aaaatgaaaa
    10441 ggaggagcag atttaagtag gtaaactttt tgtaaaacat gataattaac ctttatatta
    10501 aacaattgcc tgcagaatga ttaatcggca atgattctgt accgaattcc aaactcaaca
    10561 aaatcggctt tggcagccca atgggcttta attgcccacg aagcgtatag gttttgggag
    10621 agctgaaagc gcattccaac cttgttggta atgtacccgt cgggtttgta gcctcgtttc
    10681 aggtaaattc cgggctggaa aacaaacata gttcttccca gctgcataat gtaggcggga
    10741 ttgagagtaa ctgcatagga atcggatgac ctgtatgtgc tatcatcctt agcttgatag
    10801 gtgtacttaa ttgctttatc cttataggca gcgagctcaa ctccagccag agaggttttg
    10861 ctcaaacgtc ttagccattc aacggacatg ccgtaaacag ggtagaggtt ggtattttca
    10921 cgagaaacag atttggcact tcccagaact ccaatgctaa cgtatgttcg ggttgaatcg
    10981 gaatggtagc cgggggcctg tgttactagt aacctatcgt ctgatatgga gtactgcagt
    11041 ccaaaaccgc tggtaatcag gttaaaaccc ttgttgggtt ccctcaggtt gccattggag
    11101 gcatggctga agcccagtgc ccaacgtaga ttcaggttgg agtttaggcg gtagtaaagg
    11161 gttaaatcgt actgaaagaa aacgttgaaa ggagttccaa taaccatgtt gtaggggttg
    11221 gaatgcaaat caaactgctt ggtgaggtag ctcatcccaa atgcagcccg gttgccaata
    11281 ttaaacctat ttttccgggg caaaaactgt cgatcgataa aaacgtaggc tgcaagcatt
    11341 ttaccataga tacgatcgtt gccaagcccg gagtagaaga acccgccacc cagctgcggg
    11401 tagttgaaac tcttatgcca gtccttatat cccgtagtgt ttagcccaac attcagctga
    11461 aagctgttca cattgctctc aagaaagtag gccatgtagt cgtgatgggg catcacgaat
    11521 ccccatcgcg gttcaactcc gaaaaaaagc ctatccctaa taggagcatc ctgcgcatct
    11581 gctccagtga taacagccag caatcccatc gccgaaaatg cgacttttag ccttaaacct
    11641 ttaccaaacc aacccatagc acaatgattt actgtatttt atatcttacc aataattcta
    11701 cgacctggtt gtactgcccc ctgttcatcc tgtaggtaat atttccctta tagggacgaa
    11761 tgggaaagat agagtagctg aagaatgccc cgaaagccca atgttcgcta tagttatact
    11821 ccacaccgca cagcatggcc agttcaaact gattaaatgc cgagatatcc tcctcgggaa
    11881 aggggccgag ctcagtcatt tcctccgcct ttgctaggta tcccaggcta attccaccaa
    11941 gcgtctgaaa gccattcaca aagcgatacc cccccataac aggtagctca aagtagtgaa
    12001 gcctcatccg gtagtaacca ttgaactcat taactgttgt tttggcatag cttccctttt
    12061 gagcatagcg taattccatc atgcccaacc agctgttacc aaggtttcgg cccacccaca
    12121 ctccggccac cgggccagcc ttatcaaaac cgccgtaggt gtcgccgtca acctgggttg
    12181 cagctatacc aagagtgaaa cctgccttaa acccttgtga aaacaccaca gctgggcaag
    12241 ccgaaataat cagtataacg caaagttttc ctggtttttt caattcttaa taccttttta
    12301 ttaataccac caatgcacga ctacccatag cagctggaaa aaactgattt ccaacaggag
    12361 gcataataag acccaggctt tccgccaatg caactcctcc tgaatgaaat acataaaaat
    12421 ttgaaaagga aaacaaaagt agtaaatctt ccgcttttag acgatagcta agaaaccaaa
    12481 tgttttcgta tgaggggatt actcccccgc cctctcacac cacccggctt ccgcctgcgg
    12541 cgggcacggc ggttcctttt aacg
//
LOCUS       DAOPHX010000075        12461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_6007_length_12461_cov_4.803482, whole genome shotgun sequence.
ACCESSION   DAOPHX010000075 DAOPHX010000000
VERSION     DAOPHX010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 12461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12461
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6007_length_12461_cov_4.803482"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="PK502_06770"
     CDS             <1..849
                     /locus_tag="PK502_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00555.1"
                     /translation="LGRFLSPDSYVQAPDFTQSFNRYSYCLNNPLIYTDSDGEFFLGT
                     IFTFVGDFLRTTLFHGGLSFNKNVRREAWKDFDPSASWSATNKAWKIDIGGFKTDPHR
                     TIVGRGLQFLSRWTWELPQTVLGKGYSHIRNMTGNVDDVSYYGGATLVNKNNNSRERW
                     GLTLGPYINSKNVRADPYRDPLFRHEYGHTLQSRLVGPLYLTHVGLPSLFGSGLDNLG
                     LSDHNREWYETQANRMAERYFSNHDPGALTALPWDDNRYPREYNPNWYWTFAHPPLPF
                     MWWLFF"
     gene            868..1263
                     /locus_tag="PK502_06775"
     CDS             868..1263
                     /locus_tag="PK502_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00556.1"
                     /translation="MKMNILIILSIMLCGASCEKLVDHVYSIKIQNNTNDTLLFYESY
                     NYPDSSIVQNKPILTRVYPKDYSYLDSKKDWDEVLVSPKDTISIFILSKGTVDKYSWE
                     AIRADYKILKRYDLSIQDLENNQWTITYP"
     gene            complement(1551..2495)
                     /locus_tag="PK502_06780"
     CDS             complement(1551..2495)
                     /locus_tag="PK502_06780"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HPH00557.1"
                     /translation="MKTTQYLIAFGLQLLLLGCGDKANREEVIVTNAEEQAYFAAETS
                     PAESKSVIQESSSDFSGSSNVSAKIIRHGNINIEVKDYLVARQQIDSLANLYEAAVIS
                     ESFQENETQRGNSISIRVPAEKFDLLLGELAKIAHRVDYQQISTEDVTTEYIDVNTRL
                     QNKMKVERNYQRLLSQATSVDDILKIENQLGAVRAEIESLQGRMNYINHQVSKSLITV
                     YVYQKVEYRYRPDEMPSFGERIKQAINAGWKGLVWLVVFIIKIWPIWLAALVVFVLVN
                     RLRVIGRRRKAQKKEQRRLEKKLRKYRNDKPSDTNPQQ"
     gene            complement(2664..2906)
                     /locus_tag="PK502_06785"
     CDS             complement(2664..2906)
                     /locus_tag="PK502_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HPH00558.1"
                     /translation="MEVNFELEGEYIPLDGLLKRCSLASSGAEAHLLVDDGKVKLNGK
                     TESRRRAKVHPGDTVQACGHTIHISASNSNRFDEIS"
     gene            complement(3035..4279)
                     /locus_tag="PK502_06790"
     CDS             complement(3035..4279)
                     /locus_tag="PK502_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00559.1"
                     /translation="MKQQIIENINNPENLERLYRENRQEFSESFAEIADGYNSELVGF
                     WKVRLANENTTRSKGLLWVDLSVALAIALITGLLVKLPDFFEGIVDETFYIRNLAIIV
                     FNGIIVYTLWQNRISGWKRLLAYGMTIVALIIYSNLLPHRDSDSINLVFIHLPLLLWC
                     LFGLAFVSFDYRSIPRRVEFIRFNGELAVMTGLILIAGVLFSVVTVGLFSAINMNIEE
                     VFMKNAAIFGGVAAPIISYCLIKIYPSITRKVAPVIARIFSPLVLITLAVYLVSMIFS
                     PKSIMEDRNALMIFNVMLLAVVAIIVFSISELDKSRKKDRNVLVLLLLAALAIVTNII
                     ALVAITARVSHGLTPNRTVVLASNILILINLVLLARDLFLSYFRDKETERVEQTMAGY
                     LNIYFYWTLVVIFILPFAFGFR"
     gene            complement(4759..5049)
                     /locus_tag="PK502_06795"
     CDS             complement(4759..5049)
                     /locus_tag="PK502_06795"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPH00560.1"
                     /translation="MSNHNTKSLGEAIREFITAMKWDEKINETSIIGDWEKIVGRIVQ
                     MHTTKIKLKDGQLTIQVDSSALRHELHMRKQQLIKRINDFYHASIVSDIVLR"
     gene            complement(5054..6163)
                     /gene="recF"
                     /locus_tag="PK502_06800"
     CDS             complement(5054..6163)
                     /gene="recF"
                     /locus_tag="PK502_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130439.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HPH00561.1"
                     /translation="MHLKHLSAINFKSFRQVELELNPKINCFIGNNGQGKTNLLDAIY
                     YLSMCKSAFNSSDRQVLRHGEQFFVIEGSYQRVSSNEDIYCCFKLDEGKIFRRNGKTY
                     DRLADHIGFIPIVMVSPTDSALIDESGEERRKYMNGVISQYDRVYLDEVMRYNRLQAQ
                     RNSILKNHPHVSFSYDLLEAIDDQLVRYAQTIHAKRSSFVEQLIPIFMDYYNRVSGGE
                     ERVSIAYRSHLNDGDFKTMLRNNIQRDRTLQFTSIGIHRDDMVLSIDGYPIRREGSQG
                     QQKTFLIALKLAQFEFLSRVSGIKPLLLLDDIFDKLDMDRVGFLINQVAHDGFGQIFI
                     TDTSKNRLDMILNRLNSGFRLFSIENSEVSIIAST"
     gene            6289..6981
                     /locus_tag="PK502_06805"
     CDS             6289..6981
                     /locus_tag="PK502_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPH00562.1"
                     /translation="MAKNNKNAQPDTVESIDNALSQTERFIEQNQKSLTIIIVAILVI
                     ISLYFGYKRFYLVPKEDEARSQIFQAEYYFEKDSFKLALNGNENDLGFVDIADNYSMT
                     KTGNLACYYAGICYRQLGDFNKAIEYLKKFDAGDIMVTPIAYGAIGDCYVELKQLDEG
                     VKFYIKAAEYSDNDFTSPFFYKKAGLIYEVMNNNGDALKIYQTIKDKYPKSNEARDIE
                     KDIARNKVILQK"
     gene            7093..7596
                     /gene="ribH"
                     /locus_tag="PK502_06810"
     CDS             7093..7596
                     /gene="ribH"
                     /locus_tag="PK502_06810"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPH00563.1"
                     /translation="MATKLKNLSAYDPAEIPSAKKMKFGIVVSDWNEEVTKALLEGAY
                     QTLIGNGVKEDNILVKHVPGAFELTLGAQFMAEYADLDGVICLGCVIQGETRHFDFIC
                     QSVTQGITQLNMDYNIPFIFGLLTTENLEQALERAGGKHGNKGVEAAITAIKMAHLQQ
                     EMEQLEG"
     gene            7994..8350
                     /locus_tag="PK502_06815"
     CDS             7994..8350
                     /locus_tag="PK502_06815"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HPH00564.1"
                     /translation="MEHINYTSENERTFAMLCHLSALAGCIIPLGHILGPLIVWLIKK
                     DEFPEVDRQGKAALNFQLSVTIYGLIAGVLVILLVGFVLLAILAVFTLVLVIVASVKT
                     ANAEKFEYPLAITFLR"
     gene            8485..8766
                     /locus_tag="PK502_06820"
     CDS             8485..8766
                     /locus_tag="PK502_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HPH00565.1"
                     /translation="MFRTNEYFDGNVKSIAFETPDGRATLGVMAKGEYEFSTSTIEEM
                     TVVSGEMSVLLPGETSWKKYVEFETFIVPKDSRFRVRVDGDTAYRCYYR"
     gene            8845..9225
                     /locus_tag="PK502_06825"
     CDS             8845..9225
                     /locus_tag="PK502_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPH00566.1"
                     /translation="MAGSKGSKYYDVYLDYSIFLKRRGSNELVLSQEQIELLLLVDEL
                     QSITASARQMCISYRKAWELVRQSEQNLGFTIISKSRGGKNGGSSVLTPDGKLLANAY
                     RQLQADFNSSVKRVVKEFFQTINR"
     gene            9231..10184
                     /locus_tag="PK502_06830"
     CDS             9231..10184
                     /locus_tag="PK502_06830"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPH00567.1"
                     /translation="MRKIKFLLLLIAPLVVLQCHSGRSKQKELIVFHAGSLSLPMKAA
                     ADSFMKLHPDVRILLEAAGSMECARKISELHKPCDLFVSSDYKVVEMLIDQGFATWCI
                     PFASNEMVIAYHDKSRLADRINGSSWPSVLLSKEVAFGRSDENSDPCGYRTIMTIRLA
                     EDLYHRPGLADSLLAKDVKHIRPKEVDLIALLEANVIDYLFIYKSVAQQHGLNYLELP
                     AAINLSSKEYESVYSRVSVEVVGSTPDARVALTGEPMVYGLTIPRNAPNFDIAVQFTA
                     FFLSENSGMRILSAMGQTPIIPTSTKTYNYIPQALRAFATP"
     gene            complement(10248..11954)
                     /locus_tag="PK502_06835"
     CDS             complement(10248..11954)
                     /locus_tag="PK502_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HPH00568.1"
                     /translation="MALNPRKILIRDVTLRDGQQSLFATRMTQKQVEQVLPYFKEAGF
                     YAMEVWGGAVPDSIMRYLNEDPWYRLESIKKVIGDSTKLTALSRGRNLFGYNPYPEEV
                     IEGFNRIAVNSGISIMRIFDALNDTNNIRSTVKYVKENGGLADCTVCYTVDPKFSKKD
                     RVKAVINRKKLPGKIFTEVYYLSKAKELEAMGADMITIKDMAGLVTPDRAASIISTLK
                     KEIKVPINFHTHCTPGYGLAATLAAIVNGVDIVDTAIWNFAGGPAAPAFELIQIFCQK
                     LGIETGINLNAAATINTELKSIRTELKSFDTYPLPHEFDITHPNLPLQVDSLFEQAIQ
                     QALSAQYDELTKTCQAIESYFGFPAPDETVKHAEIPGGMYTNMLAQLKQLKLEKLLPR
                     VLEIIPTVRLDAGCPPLVTPTSQIVGAQAVNCIIDENKGLPFYTTKSTQFVNLVKGSY
                     GKTPLPIDPQFRLKITGSAEEVPFNTASYKKQPNLSFPEYGDGVKLAMNEKEELLLEL
                     FPNVTEKYLRDKRVAHFEELKRKKQEEERRRIEEEKQKYNSMTEQERWQRLQTGLISY
                     YF"
     gene            complement(12179..>12461)
                     /locus_tag="PK502_06840"
     CDS             complement(12179..>12461)
                     /locus_tag="PK502_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPH00569.1"
                     /translation="MKFMMTWLMPVMMMFWFNNYSAALSYYYLLTNLISMLQTVLMRR
                     FVDDEALLAKLHENAKKPVTKSKWQMRMEEVAKRQQELQKRQDAVRKKR"
BASE COUNT         3253 a         2668 c         2867 g         3673 t
ORIGIN      
        1 ctgggccgtt tcctcagccc cgacagctac gtgcaggccc cggacttcac ccagagcttc
       61 aaccgctaca gctactgcct gaataacccg ttaatctata cagattccga cggggagttc
      121 ttcttaggta caatctttac ttttgttggg gattttctta gaacgactct tttccatggc
      181 ggtctttcat ttaataaaaa cgtaagaagg gaggcttgga aggactttga tccttctgct
      241 agttggtctg ctactaataa agcgtggaaa atagatatag gaggatttaa aacagatccg
      301 catcgcacta ttgtaggcag aggtcttcag tttctttcaa gatggacatg ggaattgcct
      361 caaacagttc ttggaaaggg atactcgcac attagaaata tgacaggcaa tgttgatgat
      421 gtgagttatt acggaggggc taccttggtc aataaaaata ataattcaag ggaaagatgg
      481 ggacttacat taggccctta tataaattct aagaatgtta gggcagaccc atatagagat
      541 cctttgtttc gtcatgaata tggccataca cttcaaagcc gtttagttgg cccattatat
      601 ttaactcatg taggattacc aagccttttt ggttccggtt tagataattt agggcttagc
      661 gaccataaca gggagtggta cgaaacgcag gcaaaccgga tggctgaacg ctattttagt
      721 aaccatgacc ccggagcact aactgcttta ccttgggatg ataacaggta tccaagagaa
      781 tataatccta attggtactg gacatttgca catccaccct taccatttat gtggtggtta
      841 tttttctaat caattaacat taaaattatg aagatgaata ttttaatcat actttcgata
      901 atgctttgtg gtgccagttg tgaaaaattg gttgaccatg tttatagtat taaaattcaa
      961 aataatacta atgataccct gcttttttat gaaagctata attacccaga ctcttcaatt
     1021 gttcaaaaca agcctatatt aacaagggtt tatcctaaag attatagtta tttagatagt
     1081 aaaaaagact gggatgaagt cttggtctct ccaaaagata ctatcagcat atttatttta
     1141 agcaagggta cggtagacaa gtatagctgg gaagcaatta gggctgatta taaaatttta
     1201 aaacggtatg acttgagcat acaagattta gaaaataacc aatggacgat aacgtatcca
     1261 taacaaaaaa gcctcggaaa accggggctt tttgcttata gtgcagcccc gtttttctaa
     1321 aagaaatcct taaacccctt acgggtaggc atcttttcga tcagccggga aatggtttgc
     1381 ctgtcttccc cgttaggctg gcggacagga tgctgcacct gcccggggta tgcaggccgc
     1441 cggccctcac ctttgcccgg tgtagtttta cgcccatatt aatgggtttt tatattcaaa
     1501 attttatggt attggcctct gcaccttaag cagctcctgc ttcaacgggc ttactgctgt
     1561 ggatttgtgt cggaaggctt atcgttccta tattttctaa gttttttctc cagcctcctt
     1621 tgctccttct tttgggcctt gcgtcttctc cctattacac gtaggcggtt aactagcacg
     1681 aataccacca gggcagccag ccaaattggc caaattttga taatgaaaac aaccagccaa
     1741 accaaaccct tccagcctgc attaatggct tgctttattc tttcaccaaa gctgggcatc
     1801 tcgtcgggcc tatacctgta ctcaactttt tggtaaacgt aaaccgttat caggcttttg
     1861 ctaacctggt ggtttatgta gttcatacga ccctgaagcg attcgatttc agcccttact
     1921 gcacctagct gattttcaat ttttagaatg tcgtcaacac tggtagcctg ggacagtagt
     1981 ctttgatagt tcctttccac cttcatcttg ttttgcaggc gggtattcac atcaatgtac
     2041 tcggtggtta catcttcagt cgaaatttgc tggtaatcaa ccctgtgggc aatttttgcc
     2101 agctctccca gaagtaggtc aaacttttcg gccggtaccc taatgcttat gctgttgccc
     2161 ctttgcgttt cgttttcctg aaagctttcc gaaataacag ctgcttcgta caggttggca
     2221 agggagtcta tttgctgtct ggcaaccagg tagtccttga cttcaatgtt gatgttgccg
     2281 tggcggataa tcttcgcaga aacattcgag gaacctgaaa aatcggaaga actctcctgt
     2341 atgactgatt tactttcagc tggcgaagtt tcagcagcaa agtatgcctg ttcttcagca
     2401 ttggtgacta taacttcctc cctgttagct ttgtccccgc aacctagtag taggagctgt
     2461 agcccgaagg cgatgagata ttgggttgtt ttcattctgg taagaattat taatcctaat
     2521 aatttcgatt tatgtcaggc aatttttttg tattggttcg tcggcaaata ccgataatac
     2581 ctccggtagc catgcgaaaa ggtttgcacc tgcttgtagt aaacatctgc tgttgttatg
     2641 ttggcagctg ttgtatgaag atgtcagctt atttcatcaa aacggttgct attgctggcc
     2701 gaaatatgaa tggtatgacc acatgcctgt acggtatcgc ccgggtggac tttagccctc
     2761 ctgcgcgact cggttttccc gttaagcttt acctttccat catcaaccag caggtgagcc
     2821 tccgctcccg aagaagccag gctgcatcgc tttaacagcc cgtctaacgg tatgtactca
     2881 ccttccaact caaagttaac ttccataacc tatacctgca tatttagtaa atataacatg
     2941 ttttcccgga tagctgagcg gaattttatg cgttcatgca ttacgggcta attacccggt
     3001 ttcgtaatgg gatgcgtggg tttagcctta ccggctaccg aaacccaaag gcgaagggca
     3061 gtatgaatat caccacaagc gtccagtaaa agtaaatatt caggtaaccg gccatggttt
     3121 gctcaaccct ttcggtttct ttgtcccgga agtatgatag gaagaggtca cgtgccaaca
     3181 gcactaggtt tatcagtatc aggatgttgg atgccagcac aaccgttctg tttggagtta
     3241 aaccgtgtga aactcttgct gttatggcaa ccagcgcaat tatatttgta acaatagcta
     3301 gtgctgcaag taggaggagc accagcacgt ttctgtcctt cttcctcgat ttatcgagtt
     3361 cggagatgga gaacacgatg attgccacta ctgccagcag catcacgttg aaaatcatta
     3421 gggcgtttct atcctccatg atgcttttgg gtgaaaagat catcgaaacc aggtaaacag
     3481 ccagcgtgat gagcactaac ggggagaata tccgggctat aaccggcgcc acctttcggg
     3541 tgatgctggg gtatattttt atgaggcagt aggagataat tggtgccgca actccaccaa
     3601 atatggcagc atttttcatg aatacctctt caatattcat gttgatggcc gaaaacaacc
     3661 ccacggtaac taccgaaaac agcacaccgg caatcaggat taacccggtc ataaccgcca
     3721 gctcgccgtt aaaacgaatg aactcaacac gccttggtat gctccggtag tcaaatgaaa
     3781 cgaaagctaa cccgaacagg caccaaagca gcagtggcag gtggatgaaa accaggttaa
     3841 tggaatcgct atccctgtgg ggcaacaggt tggagtatat aatcagcgca acaattgtca
     3901 taccgtaggc taaaaggcgc ttccatccgg aaatcctgtt ctgccagagg gtgtaaacaa
     3961 taatgccatt aaagacaata atggccaaat ttctgatgta aaaggtttca tcaacaatac
     4021 cctcaaaaaa atcgggcagc ttgactagca gtcctgttat cagggcaatg gctaatgcta
     4081 ctgacaggtc aacccacagc aaacccttac tccgggtggt gttttcattg gccagtctta
     4141 ccttccagaa accaacaagt tcggaattgt agccgtccgc tatttcggca aatgattcgc
     4201 tgaattcctg cctgttctcg cggtaaagcc gctccaggtt ttctggattg ttgatatttt
     4261 ctattatttg ctgcttcatt ttttaggtat gttggtgttt tatttgcagt ttaaagccct
     4321 tgtaccgggg caagtttaaa tatatgtggc atgaaaacca tcagggcaaa tctacaaatc
     4381 gtcattcggg tgaaatttgg ccatttaatg gttatttcta ctttttaaaa cggtcaacca
     4441 ggctgtttta tttttttaat gcacctgtct ccagcaaatt aattgttagc aaccggaaaa
     4501 atgaattttt ttgctctata aaggtagctg gtacattgca aaaatgtaca caaaggattg
     4561 cgtttataac attctttagc agagcattac cgaattaagt ggcatttatt tttaccggta
     4621 aaggaagcag ctgcaaaagt tctgaggttt atttagaacg gcttttgtaa cgatgatgtt
     4681 ggctggcttg ttgtaatggt attgtctatc caaagcttca ttctggtaag tggttacaac
     4741 aaatgtttat agcaagggct acctgagcac gatatcactt acaattgagg catggtaaaa
     4801 gtcgttaatt cttttgatga gctgctgttt acgcatgtgg agttcatggc ggagcgccga
     4861 ggagtcaacc tggatggtga gctgtccgtc ttttagcttg attttggtag tgtgcatctg
     4921 cacaattcgc ccaacaattt tttcccagtc gccaatgatg gaggtttcgt ttattttctc
     4981 gtcccacttc atggcggtaa taaactccct gatggcttcg cccaacgact tggtgttatg
     5041 gttgctcatg gttttatgtt gaggcgatta tggatacttc tgagttttcg atactaaaaa
     5101 gccggaatcc ggagttgagc ctgtttagta tcatatccag ccggttcttg ctggtatcgg
     5161 tgatgaaaat ctggccaaat ccgtcatggg caacctgatt aatcagaaag ccaaccctgt
     5221 ccatatcgag cttatcgaag atgtcatcga gcagcagcag gggtttaatt ccggagactc
     5281 tcgacaggaa ttcgaattgt gctagcttaa gggcaatgag gaaggttttt tgctggccct
     5341 gtgaaccctc gcggcgaatg gggtacccgt caatgctaag caccatgtcg tcgcggtgta
     5401 tgcctatgga ggtaaactgc aaagttctat ccctttgaat gttgttgcga agcatggttt
     5461 taaagtcgcc atcgttgagg tgcgacctgt aggctatgga caccctttct tctccgccgg
     5521 ataccctgtt gtagtagtcc atgaaaatgg ggatgagctg ctcaacaaag ctgctgcgct
     5581 tggcatgaat ggtttgggca taccggacaa gctggtcgtc gatagcctca agcaggtcgt
     5641 agctaaagct tacatgcggg tggttcttca ggatgctgtt gcgctgggct tgtagtcggt
     5701 tgtagcgcat cacctcgtcg aggtaaaccc tgtcgtactg cgaaataaca ccattcatat
     5761 acttacgtcg ctcctcacct gactcgtcaa taagcgccga atcggtgggg gagaccataa
     5821 cgattggaat aaagccaata tgatcggcca gccggtcgta ggttttcccg tttcgtcgga
     5881 aaatcttacc ttcatcgagt ttgaaacagc agtaaatatc ctcgttgctc gacacccttt
     5941 ggtagcttcc ctcaattaca aaaaactgtt ctccgtgcct gagcacctgc ctgtcgctcg
     6001 agttaaaggc gcttttgcac atcgaaaggt agtaaatggc atccaacagg tttgtttttc
     6061 cctgtccgtt gttgccaata aagcagttga tcttggggtt aagttccagc tcaacctgtc
     6121 tgaacgattt gaagttaatg gccgatagat gtttaaggtg catggcaggt ggagactttt
     6181 gctatgctac aaaggtaatt atttgtaaaa tgttggcgca aagttttcag atattccaaa
     6241 atccattact tttgcgtgga cttaaatcat tactaacccg ttttaacaat ggcaaagaat
     6301 aacaaaaatg ctcaacccga cacggttgag tctattgata acgctttaag ccaaactgaa
     6361 aggtttattg agcagaatca gaagagttta accatcataa tagtggctat actcgtcatc
     6421 atcagcctgt actttgggta caagcgcttt tacctggtac ctaaggagga tgaggctcgt
     6481 tcacagatat ttcaggctga atactacttt gagaaggact cgttcaagct cgcccttaac
     6541 ggcaacgaga acgacttggg atttgttgat attgccgata actacagcat gaccaaaacg
     6601 ggaaatctgg cctgctacta cgccggcatt tgctaccgtc agcttggcga cttcaacaag
     6661 gccatcgagt accttaaaaa gtttgatgcg ggtgatatta tggttacgcc aattgcctat
     6721 ggcgctattg gcgactgcta tgtggagctt aagcagcttg atgagggggt aaagttctac
     6781 atcaaggcag ccgagtacag cgataacgat ttcacctcac cctttttcta caaaaaggca
     6841 ggcttaattt atgaggtgat gaacaataac ggcgatgccc ttaagattta ccagaccatt
     6901 aaggataagt atcccaagag caacgaggca agagatattg agaaggatat tgcccgaaat
     6961 aaggtaatac ttcaaaaata gccatgatga ggggtgctgc ttgtaggcac ccctttctat
     7021 ttattatttc cctttatgtt gacttgttgg aaatcatagc ttaactttgg taaaattaat
     7081 agctgcagta acatggcaac gaagcttaaa aatctatccg cctacgatcc cgctgaaatt
     7141 ccttcggcaa aaaaaatgaa attcggaatt gtggtatccg actggaatga ggaggttacc
     7201 aaggcgctgc ttgagggtgc ataccaaaca ctcattggta atggcgtcaa ggaggacaac
     7261 atactggtta agcatgttcc gggagctttt gagctgaccc ttggggcaca gttcatggcc
     7321 gaatatgccg acctcgatgg ggtgatttgt ctgggttgcg ttattcaggg cgaaacccgc
     7381 cacttcgatt tcatctgcca gagcgttacc cagggaatta cccagctgaa tatggattat
     7441 aacattccct tcatcttcgg cttgttaacc accgaaaatc tcgaacaggc tttagagcga
     7501 gccggcggaa aacatggcaa caagggcgtt gaggctgcaa tcaccgcaat taaaatggca
     7561 cacctgcagc aggagatgga gcagctggaa ggataagcac aaccaccccg gcgggatttc
     7621 ttgccagtat aggatttgcc attttacttg caacaaggtt ttaccttgtt ccaaccaccc
     7681 aaggttttaa gatttttagt tgactataag ctgcgatttg gcattctgca cttgtttacg
     7741 ctccgatgaa tttgggaggg ctcaccgtag gggtaataat atttgtttgg acttgccgaa
     7801 ctagcaaata atgcccggtt attaatccga ttgtttacaa gccttactac atgggtattg
     7861 caggctaact tcattctcgc atggggtaat ctccacctct tattttttgt tttttgctaa
     7921 aatttctttc tatcctgcag caagattgct taattcgtag tcttatattt gtaatctacc
     7981 aactggaatt tgtatggagc atatcaacta cacatcggag aatgagcgaa cttttgccat
     8041 gctttgccat ctttcggcgt tggctggctg tatcattcca ttagggcaca tccttggccc
     8101 gctgatagtt tggcttatta aaaaggatga atttcctgaa gttgataggc agggaaaggc
     8161 tgcgcttaac tttcaacttt ctgtaaccat ctatgggctc attgcagggg tgttggttat
     8221 tctgctggta ggctttgttc ttctggcaat tctggctgtg tttaccctgg ttttagttat
     8281 tgttgcttcg gtaaaaacag ccaatgctga aaaatttgaa taccccttag ccataacctt
     8341 tcttcgctag cccgaatcac ctcgtgcttt atagctgtgt ttggtgggct ggtaggtgtc
     8401 agaactctac aacttaaaaa acttttagtt tagggtatat taccaaaatc aattttggta
     8461 tatatttgag gaaaacataa ggctatgttc aggacaaacg agtactttga tggtaatgta
     8521 aagtcaattg cctttgaaac tcccgatggt agggctactc tgggtgttat ggcaaaaggc
     8581 gagtatgaat tcagcacatc tacaatcgag gaaatgacgg ttgtttcggg tgaaatgtcg
     8641 gttttgcttc ccggtgaaac cagctggaaa aaatatgtag agtttgaaac atttattgtt
     8701 cctaaagatt cccggtttag ggttagagtg gatggcgata ctgcttaccg ttgctattac
     8761 aggtaattta atcggctact cctttttcag tcagtttctt aaaccctggt ttttgaaatt
     8821 ggttagtaaa ctcacccgaa tattatggcc ggttcaaagg gctcaaaata ctatgatgtt
     8881 tacctcgact acagtatttt tttaaagcgt aggggtagca acgagcttgt gctatcgcag
     8941 gagcaaatag agctgctgtt gttggttgat gagcttcaat ccattactgc ttcggctagg
     9001 cagatgtgta taagctaccg taaagcgtgg gagcttgtga ggcagtcgga gcaaaacctc
     9061 ggttttacaa tcataagcaa atcaaggggt ggcaagaatg gcggcagctc tgttctaaca
     9121 cccgatggca agcttctggc caatgcatac aggcagctgc aagccgattt caactcctcg
     9181 gtgaagcggg tggttaagga attctttcag acaattaacc ggtagcaacc atgcgaaaaa
     9241 ttaaattttt actactgcta attgctccgt tggtggtgtt gcagtgccat tcaggtagaa
     9301 gtaagcaaaa agagctaatc gtttttcacg cgggaagcct ctcgcttccc atgaaggcgg
     9361 ctgccgacag ctttatgaag ttacatcccg atgtcagaat cctgcttgag gcagccggta
     9421 gcatggagtg tgccagaaag atttcggagc ttcataaacc ctgcgacctg tttgtatcat
     9481 ccgactataa ggtcgtcgaa atgcttattg accagggttt tgctacatgg tgcatcccct
     9541 tcgccagcaa cgaaatggtt attgcctacc atgataagtc caggcttgcc gaccgtatta
     9601 acggttccag ctggccatcc gtgcttttga gcaaagaggt agcctttgga agaagcgatg
     9661 agaactccga tccatgcggt tatcgaacaa ttatgactat tagacttgcc gaggatttat
     9721 accatcggcc aggcctggcc gattcgcttc ttgccaagga cgtgaagcat atcaggccta
     9781 aggaggttga cctgattgcg cttcttgaag ccaacgtaat tgactatttg tttatttata
     9841 agtcggtggc gcagcaacat ggactaaact atcttgaact ccccgctgca attaaccttt
     9901 ccagtaagga gtacgaatcg gtttactcca gggtttcggt agaggtggtt ggtagcacac
     9961 ctgatgcaag ggtagcttta accggtgagc ctatggtata tgggttaaca attccccgta
    10021 atgcaccaaa ttttgatatt gctgtgcagt ttacagcgtt ttttctaagc gaaaatagtg
    10081 gtatgcgcat tctttctgcg atggggcaaa ccccgataat ccctacatca accaaaacct
    10141 ataactacat accacaagct ttacgggcat ttgcaacccc gtagtacaaa ttcaactcat
    10201 caaggctgtt tgtatagctc cttcggcctc actagaacag tgcaatttta gaagtagtag
    10261 cttattaatc cggtctgtag ccgctgccac cgctcctgct ccgtcatgga gttgtatttc
    10321 tgcttttcct cctcaattcg ccgacgttct tcctcctgct ttttacgttt tagctcctca
    10381 aagtgagcca cacgtttatc cctaagatat ttttcggtaa cgttggggaa aagctccagc
    10441 aacagttctt ccttttcgtt catggccagc ttaaccccat cgccatactc gggaaatgaa
    10501 aggttgggtt gcttcttgta gctggcagtg ttaaaaggca cctcttcggc agagccggta
    10561 attttcagac gaaattgggg gtcaatggga aggggagttt tgccgtagga gcctttgacc
    10621 aggttgacaa attgggttga ttttgtggtg tagaaaggaa gtcccttgtt ttcgtcgatt
    10681 atgcagttaa cggcctgcgc tccaactatt tgactcgtag gggtaacaag tggagggcaa
    10741 ccagcatcca aacggacagt tggaataatt tcgagcacgc gtggaagaag cttttccaac
    10801 tttagctgtt taagctgagc cagcatgttg gtgtacattc ctccaggaat ttcggcatgc
    10861 ttaacggttt cgtcgggagc ggggaagcca aagtaactct caattgcctg gcaggttttg
    10921 gttagctcgt cgtattgggc cgataaggct tgttgtatgg cttgttcaaa aaggctgtca
    10981 acctgtaaag ggaggttagg gtgggtgatg tcaaattcgt ggggtagggg gtaggtatcg
    11041 aagcttttca gctcggtgcg gatgcttttt agctcggtgt tgatggttgc tgctgcgtta
    11101 aggttaatgc ccgtttcaat tcccaacttt tggcagaaaa tttgaatcag ctcgaatgca
    11161 ggagcagcag ggccgccggc aaagttccaa attgcggtgt caacaatatc aacaccattg
    11221 acaatggcag caagggttgc agccaatcca tatccaggtg tacagtgggt gtggaagtta
    11281 ataggaacct tgatttcttt tttaagcgtc gaaattatgc tggcggccct atcgggggta
    11341 accagtccgg ccatatcctt aatggtaatc atatcggcgc ccatggcttc cagctccttt
    11401 gccttcgata agtagtaaac ttcagtaaaa attttacctg gaagtttttt cctgttgatt
    11461 acggccttaa ctctatcctt tttggaaaat ttggggtcaa cggtatagca aactgtgcag
    11521 tcggccaaac cgccattctc cttaacatac tttacggtgc tacgaatatt gttggtgtca
    11581 ttcagggcat caaatatcct cataatggat atgcctgagt ttacagcaat tctgttgaat
    11641 ccctctataa cctcctcagg gtaggggttg tagccgaaaa gatttcgacc acgtgacagg
    11701 gctgtcagct tagttgagtc gccaattacc tttttaatac tttctagccg gtaccaggga
    11761 tcttcgttca aatacctcat tatggaatcg ggaacagcgc cgccccaaac ctccattgcg
    11821 tagaatccgg cttccttgaa gtaaggtaaa acctgttcaa cctgtttttg ggtcattcgt
    11881 gttgcaaaca gcgattgctg cccatcgcgc agggttacat ccctgatgag tatttttctg
    11941 gggttgagtg ccatggtgta gttgttgtat tgaagttgac ttgctagttg ctgatgcgct
    12001 aacgcatact ttattgtttg ttacggcaaa gaaactaata agttatctga aacccgcata
    12061 aatatttgaa gttctacaac atagctcttt aacatattta tataacataa aaaaaggggc
    12121 taaactggcc cctttatact agtagctata taactagttt gtattcagca tgttgaaatt
    12181 atcgcttttt ccgtacagca tcctgacgct tttgtagctc ctgctgacgc ttagccactt
    12241 cctccatcct catttgccat tttgatttgg ttacgggttt tttggcattt tcatgcagct
    12301 tggccagcaa ggcttcgtca tcaacaaaac gtctcataag cacagtttga agcatggata
    12361 taaggttggt aagcaggtag tagtagctaa gggctgcgga gtagttgttg aaccaaaaca
    12421 tcatcataac aggcatcaac caggtcatca taaacttcat t
//
LOCUS       DAOPHX010000076        12444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_6018_length_12444_cov_7.041327, whole genome shotgun sequence.
ACCESSION   DAOPHX010000076 DAOPHX010000000
VERSION     DAOPHX010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 12444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12444
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6018_length_12444_cov_7.041327"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            371..778
                     /locus_tag="PK502_06845"
     CDS             371..778
                     /locus_tag="PK502_06845"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaC family regulatory phosphoprotein"
                     /protein_id="HPH00570.1"
                     /translation="MISKEGLKGLVDLHIQATTETPEVICSKSTGKISMVGNLIPSDP
                     ESFFAPITRWFLLYSTEFEPKQITVDLYLTFINGCSEHHLVSFLKKVEELYSRGVEVR
                     INCHYESDDFDMRDWGRDLRLVLKIPVDMVEIN"
     gene            complement(803..2305)
                     /gene="hutH"
                     /locus_tag="PK502_06850"
     CDS             complement(803..2305)
                     /gene="hutH"
                     /locus_tag="PK502_06850"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HPH00571.1"
                     /translation="MTRIHRISPQPLTWKVLEDITTGDFRLSLSDESKVLIEKCRRYL
                     DVKMEESSVPIYGITTGFGSLCNKTISRNELSQLQKNLVMSHACSTGAEVSSEIVRLM
                     LFLKAHALSLGNSGVQVATVERIIDFYNNDVLPVVYDRGSLGASGDLAPLANLFLPLL
                     GMGDVRYKGKVVEALDVLKEFGWQPIELKSKEGLALLNGTQFMSAHGVSAMLKAFRLI
                     NLSDLIGAISLEAFDGRVEPFHENIQQARPHRGQVETARHIRAILEGSELINRPKKHV
                     QDPYSFRCIPQVHGASRDAIAYVASVILTEVNSVTDNPTIWPDEDLVISGGNFHGQPL
                     ALAYDFLAIALAELGSISERRTAQLILGLRGLPEFLVANPGLNSGLMIPQYAAAAMVS
                     QNKQLCTPASVDSIVSSNGQEDHVSMGANAATKLLRVVDNVERILAIELLNAAQAMEF
                     RRPAKSSPAIESILASYRRQVKFFGEDRIMFKEIDKTLEFIRNIKPGEWL"
     gene            complement(2310..3686)
                     /gene="hisS"
                     /locus_tag="PK502_06855"
     CDS             complement(2310..3686)
                     /gene="hisS"
                     /locus_tag="PK502_06855"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPH00572.1"
                     /translation="MASSKPSIPKGTRDFNPEQMVRRNYIFDTIRSVFSIYGYQPIET
                     PAMENLSTLLGKYGEEGDKLLFKILNSGDFLERVDPSTARPGNSNAVSLAICEKGLRY
                     DLTVPFARFVVQYQNDIVFPFKRYQIQPVWRADRPQKGRYREFYQCDVDVIGSTSLLN
                     ELELVLIINEVFQRLGVRVVVKLNNRKILSGIAEVIGEAERLTDITVAIDKIDKIGID
                     GVNSELRERGVSEVAIEKLQPVILLKGNNTEKLGLLKNTLAPSEVGMRGIGEMEELLE
                     LIRQSGELLSEVELDLSLARGLNYYTGTIFEVKAADVEIGSICGGGRYDDLAGIFGMK
                     NLSGVGVSFGADRIFDVMLQLNLFPDSLLSSTQVMFTNFGKSEAAYCLKALRQLREQG
                     VSAEIYPDPAKMKKQLDYANRRRIKLVVIAGESEMQQNQLSVKNMETGEQATISAEDV
                     VSYIKENI"
     gene            4080..4811
                     /locus_tag="PK502_06860"
     CDS             4080..4811
                     /locus_tag="PK502_06860"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPH00573.1"
                     /translation="MNGQLKSLLRKALNRLHLDLTKNLRYDRLSKRIISLHLKPDSTC
                     IDVGAHRGEILDLFLQFAPKGHHLAFEPIPSLYNALEKKYSGRCRVYPFVLGDRIGKT
                     PFQLVRNAPAYSGILKRSYSIRHPEVEEIEVEMATLDSVVNEEENIDFMKIDVEGAEL
                     QVLLGARSTLCRCRPNIIFECGLGASDYYGTNPDDLYDFLTGIVGLKISTLKGWLTDS
                     GNPLTRDEFRRHFERNSEYYFFAHP"
     gene            5028..5513
                     /locus_tag="PK502_06865"
     CDS             5028..5513
                     /locus_tag="PK502_06865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPH00574.1"
                     /translation="MTVEDFNSCVDLYSDGIYRFALKMLGDTMLAEDNVQDSFERLWV
                     KRGEIDRSKAKSYLFTTSYHASVDAIRRDRHKQELENAQPVLEIGYQPNFDVKEIINH
                     AVDQLPTVQKSVLLLRDYEGYSYGEIAGITGLNESQVKVYIYRARVFLKEKLVSIENL
                     I"
     gene            5519..6538
                     /locus_tag="PK502_06870"
     CDS             5519..6538
                     /locus_tag="PK502_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00575.1"
                     /translation="MSINLNTYEEYLLDYIDGKLCPEVEAELVKFLRLHPDIASELDG
                     LNQLTIPKPNLSFSGKQRLRRTVEQDLFSCNEMDYLCIAELEGDISEVEKARLEELKG
                     QHPEVERFRNLYAHTLLLSENSHTFPNKASLKRHSIISKRRIMLTAAAAITAAIVGLH
                     TIHKLSLPDDLQPQNQMAKSAIIESQPREYRPTSTTTYPQTQPKPESRVAVSLAYKTS
                     TSTISMDNEELQPMRTNVAELKQIASKEPIIEPIASITPKPFPITGKVQGVAFREKEL
                     APNVAPSTAKVYTLADIAGLSLKRLAGLVGISYNVKTDSTNTSKHIEVDSRLIAISTI
                     IRKNN"
     gene            6611..7693
                     /locus_tag="PK502_06875"
     CDS             6611..7693
                     /locus_tag="PK502_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPH00576.1"
                     /translation="MKQLVILASSLLSFFTLTAQNEKETMLRSLEIADTLKVSDTLST
                     NHRKPNMVVNVEKTIKVADGDTVEETTSDTLVVDSNTGDNNVAYQGDTIIVDRNQGNT
                     KSDSTIIRLGEKTIVIVEGKDKTTVEIPEGLNKDFRNSFRRSNRLKFKGHWAGIEMGV
                     NGFMDADHSTSMSNELEWLDLKQSRSWNVNLNFLQYSMGFGTSWIGLVTGMGLEFNDY
                     HFNNPLTLKVVNGVTVADSSYLLAGYKVDKTKLSTVNLTLPLLVEFQIPTSESDRHKI
                     YISAGVIGGLRIGTHTKVVFDDGDRHKDKGRSDFNIATFRYGVTARAGYRSLRLFANY
                     YPTQLFEKNKGPEIYPFAVGLVLIPF"
     gene            complement(8046..9380)
                     /locus_tag="PK502_06880"
     CDS             complement(8046..9380)
                     /locus_tag="PK502_06880"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPH00577.1"
                     /translation="MSRILILGAGLSSTNLIEYLLGNAPAYGWTIRIGDLSLEAAAGK
                     LGGNPNGQAFKFDIFNAEQLSAEVAWADVAISMLPAFMHPVVAKECVRQGKHLLTASY
                     VSDAMKSLHDEARQKGVALMNELGVDPGIDHMSAMRMIDGIRNKGGKITGFISNTGGL
                     IAPECDNNPWNYKFTWNPRNVVLAGQGVTHFLENGEHKYIPYHRLYSNIRTYNIPPYG
                     EFEMYPNRDSLKYREIYGINDIQTIIRGTLRRSGYSKAWDVFVQLGMTDDTYKMEGSE
                     MLTKREFLNSFLPYHPTETVEEKLQRVIPAAQDKIIFNKLKWLGIFDNELIGMANASP
                     AQLLQRIMEEKLGLEPNDKDILIMQHIFDYELDGKKFQKKSTLTLVGEDTVHTAMAKT
                     VGTPLAIAAKMLMTGQVNDRGVVMPIKPHLYNPILNELEEYGVKFVEEERQL"
     gene            9750..10001
                     /locus_tag="PK502_06885"
     CDS             9750..10001
                     /locus_tag="PK502_06885"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPH00578.1"
                     /translation="MLTLSIGFGELVVILLVTLLVVGPREVAKVARKLGKLYRRMDNA
                     MSDLRSYVENPSSPGDNSDLVDTSEKRNDSDNANKTPAG"
     gene            10069..10722
                     /locus_tag="PK502_06890"
     CDS             10069..10722
                     /locus_tag="PK502_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPH00579.1"
                     /translation="MIKVENITKSYGALMVLKGITLEIPEKKVVAIVGPSGAGKTTLL
                     QIMGTLGRADSGQVLINGENVAQLNDRQLAKFRNTHIGFVFQFHHLLPEFTALENVCM
                     PALIAGKGMATATKKSTELLKMLGLSDRLDHKPSELSGGEQQRVAVARALVNNPTVIL
                     ADEPSGNLDSRNKEELHKLFFMLRDQLGQTFVVVTHDKDLAQMADLQVGMNDGLLCP"
     gene            10719..11267
                     /locus_tag="PK502_06895"
     CDS             10719..11267
                     /locus_tag="PK502_06895"
                     /inference="COORDINATES: protein motif:HMM:NF021210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2400 family protein"
                     /protein_id="HPH00580.1"
                     /translation="MKLPQNRAELTQLLNNRYHRYAGASFLSDDPILIPHRFTKTEDI
                     EISALLTATIAWGTRKSIIGSMNRLMAIMDNSPFEFVINFSENDEKRLNGFAHRTFNS
                     SDCIAFIRSLQRIYADGGGLKAIFTNGFRQTRTAKQALEAFRLRLIQENFSGHAMKHV
                     PNVESGSAAKRLNMLRRVIKVA"
     gene            11315..12094
                     /locus_tag="PK502_06900"
     CDS             11315..12094
                     /locus_tag="PK502_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HPH00581.1"
                     /translation="MGQDLKELKDFLDEMVTQYNNPTFIGLDPIQIPHSYTIKEDIEI
                     SGFLAAIISWGNRTSIVKNAKRMLAYMGDSPYDFILNHREQHLNQMEGFVHRTFNSVD
                     LLTFIEGLKYIYKDKNGLEGIFVKYQANDSLQPAIHEFKKEFFNIPHLERTRKHLPDP
                     MTGSAAKKINMYLRWMIRQDNAGVDFGIWKNISPSKLSCPLDTHSGNVARKLGLLGRK
                     QNDAKAVQELDGVLRQLDCHDPVKYDFALFGLGVSNDLKII"
BASE COUNT         3513 a         2994 c         2708 g         3229 t
ORIGIN      
        1 tataagctga agcttacgcc gtcagaaacc gctacttggt tacgactaag tcaaagactt
       61 agccgagcaa gtaaaccccc aaacttcaaa ggaacaggtg ggcatagagg atggctcatg
      121 aaagaaagat tccgaaaggg ctttagacga aaaccttcca gcacaccgcc tatattcata
      181 tttacaggtt agaaaagggg gtagccgctc aaaacaacct tcatgtagaa gaaaaggttg
      241 taataactat tggtatccag aaacaccaac aagggtcagg cgtaaaaaaa atcagcatca
      301 ctttttcatt tttttatccg gccattgttt gaattttttt tacattagct ccgtttatat
      361 cttcatcagc atgatttcga aagagggttt aaaagggttg gttgatctac atattcaggc
      421 aacaacggaa actccggaag taatttgtag caaaagcaca ggaaaaattt cgatggttgg
      481 gaatttaata cccagcgatc ccgagtcgtt ctttgctcct atcaccaggt ggtttctttt
      541 atactctacg gaatttgaac ccaagcaaat tactgttgac ctatacctca catttatcaa
      601 cggttgctcg gagcaccatc tggtaagttt cttaaaaaaa gttgaggagc tatacagcag
      661 gggtgttgaa gtaagaataa actgccacta cgaaagcgac gacttcgaca tgcgcgattg
      721 gggccgcgac ctacgactag tcctcaaaat tccggttgat atggttgaga taaactagca
      781 gttttcctct accgcacatc ttttatagcc attccccggg tttaatattt ctaataaact
      841 caagagtttt atcaatttcc ttgaacataa ttctatcctc accaaaaaac ttaacctgcc
      901 ggcggtagct tgccagtatg ctttcgatag ccggcgagga tttggctgga cggcgaaact
      961 ccattgcctg ggctgcgttc aggagttcaa tggccagaat gcgttcaaca ttatctacca
     1021 cgcgcagcag cttggttgca gcgttagccc ccatgctcac gtggtcctcc tggccgttgg
     1081 atgaaacgat ggaatcaacc gacgcaggtg tacaaagctg cttattctgg cttaccatgg
     1141 cggcagcagc gtactggggg atcattaaac cggaatttag cccggggtta gccaccagaa
     1201 attcaggtag cccccttaac cctaaaatga gctgagctgt gcggcgttcg gaaatgcttc
     1261 ccagctcggc aagggcaata gccagaaaat cgtaggccag ggctagcggt tgcccgtgga
     1321 agttgccccc cgaaatgacc aaatcctcat cgggccaaat ggtggggtta tcggtaacgg
     1381 agttaacctc ggtgagaatt acgctggcaa catacgcaat ggcatccctt gatgccccat
     1441 gaacctgagg gatacagcgg aatgagtagg gatcctgaac atgctttttg gggcggttaa
     1501 tcagctcgct accctccaga attgccctaa tgtgcctggc ggtttcaacc tgtccccgat
     1561 gtggcctggc ctgttgaata ttctcgtgga acggctctac ccgtccatcg aaagcttcta
     1621 gggagattgc gccaatcagg tcggaaaggt ttatcaacct aaatgccttg agcattgcgg
     1681 aaacaccatg ggcgctcata aactgggttc cattcagcaa agccaacccc tccttcgact
     1741 tcagctcaat gggctgccac ccaaactcct ttaacacatc cagagcctct acaacctttc
     1801 ccttatacct tacatcaccc atgccaagca gaggcaaaaa aagattggcc agcggagcca
     1861 agtcgccgga agctccaagc gagccacgat cgtaaacaac aggcagtacg tcgttattgt
     1921 agaaatcaat gatacgctcc accgttgcaa cctgaacacc cgagtttccc aacgacaggg
     1981 catgtgcttt aaggaaaagc attagtctaa caatttccga acttacctct gcgcccgttg
     2041 aacaggcatg ggacatgacc aaatttttct ggagctgcga cagctcattt cgggatattg
     2101 ttttgttgca aagcgagccg aacccagtgg tgatgccgta aatgggaacg ctgctttcct
     2161 ccatcttgac atccaggtaa cggcggcact tttcaatcag aacttttgac tcatccgaaa
     2221 gcgatagcct gaaatccccg gtggtaatat cctctaaaac cttccatgtg aggggttggg
     2281 gtgaaatcct atgaattctg gtcatggctt taaatatttt ctttgatata gcttacaaca
     2341 tcttcagcag aaatagtagc ctgctctcct gtttccatat tcttcactga tagctggttt
     2401 tgctgcatct cgctctcgcc ggcaatgaca accagcttaa tcctacgccg gtttgcatag
     2461 tcgagctgct ttttcatctt ggcagggtcg ggataaattt ctgcacttac accctgctcc
     2521 ctaagctgcc gaagagcttt aaggcagtat gcagcctcgc ttttcccgaa attggtaaac
     2581 attacctgcg tggaggatag caggctatcg ggaaagaggt tgagctgcag cattacgtcg
     2641 aatatgcggt cggcaccgaa cgacactcca accccgctta ggtttttcat tccaaaaata
     2701 ccggcaagat cgtcgtagcg accaccgcca caaatactgc caatttcgac atcggcagcc
     2761 tttacctcga atatggttcc ggtgtagtag tttagcccac gtgccagcga gaggtccagc
     2821 tcaacttccg ataaaagttc accgctctgt cgaatcagct caagcagctc ttccatctcg
     2881 ccaattcccc tcatccccac ctcggaaggt gctagtgtat ttttcaacag cccaagtttt
     2941 tcggtgttgt tgcccttcag taaaataact ggctgcaact tttctatggc gacttcggat
     3001 acaccccgtt ccctgagctc gctgttcacc ccatcaatac caatcttgtc aatcttatca
     3061 atggccaccg tgatatcggt taacctttcg gcttcaccaa taacctcggc aataccgctt
     3121 agtattttgc ggttgttaag cttcacaacc acgcgaacac ctaaccgttg aaacacctcg
     3181 ttgatgatga gaaccagctc cagctcgttg agaagcgagg tggatccaat tacatccaca
     3241 tcgcactgat agaactccct gtagcgtcct ttttgcggtc tgtcggccct ccaaacaggc
     3301 tgtatctggt agcgcttaaa gggaaacaca atatcgtttt gatactgaac tacaaagcgg
     3361 gcaaagggaa ctgttaggtc gtagcgtagt cccttttcgc aaatggcaag cgaaacggca
     3421 ttggagttgc caggtctggc tgtgctgggg tcaacccttt caagaaaatc gcccgagtta
     3481 agaatcttaa aaagtagctt atcgccctcc tcgccatact tccccagcag ggtggagagg
     3541 ttctccatgg caggcgtttc gatgggttgg tagccgtaaa tactgaacac cgaacggatg
     3601 gtgtcgaaaa tgtagttccg ccggaccatc tgttccggat taaaatctct ggtccccttt
     3661 ggaattgaag gcttgctgct tgccattgcc atctagtgtt tttcaagttt taatgatcag
     3721 taaccgggtt aatgctccaa cgttagctac ccgctgtcac ttaaagcgct tacctgttaa
     3781 tacccaagct gaaatttttc ggcaattggc gtatccaggt attggatatt tagtaagcga
     3841 ctgcaaacct actatttttt tgcaacccgt tgaaggggtt gtccgacaat tttagcatat
     3901 agcatgttac gccgtattta gctggttggc cacggctgtg cggctaccat aaccttaagg
     3961 ttgataggta atcatataaa ttatcggcaa attcttccta cttttacacc tcttgccctt
     4021 gctccacaac tattcctaat tttgaaaacc tgcttaaatc aacctcatgc agtttggata
     4081 tgaacggcca gcttaaaagc ctgcttagga aagccctgaa ccggcttcac ctcgatctca
     4141 caaaaaattt gaggtacgac aggcttagta aaaggattat ctccttacac ctaaagcccg
     4201 actccacctg cattgacgtg ggtgcccata ggggagaaat tcttgacctg ttcctacaat
     4261 ttgccccaaa aggccatcac ctggcctttg agcctattcc ttcgctctac aatgccctcg
     4321 aaaaaaaata ctcgggaagg tgccgggtat atccgtttgt gctgggagac aggataggga
     4381 aaaccccctt ccagctggtt agaaatgcgc ctgcctacag cggaatttta aaacgaagct
     4441 acagcatacg acatcccgag gttgaggaga ttgaggttga aatggccacg ctggactccg
     4501 ttgtcaatga ggaggaaaat atagatttta tgaaaatcga tgtcgagggt gctgaactcc
     4561 aagtgctact tggtgcccgt agcactcttt gccgttgcag gccgaatatt atttttgaat
     4621 gcgggctggg tgcaagcgac tactacggga ctaaccccga tgatctttac gatttcctaa
     4681 ccggcatcgt aggtctgaaa atttcaaccc ttaagggttg gcttaccgat agcggaaacc
     4741 cgctaaccag ggatgagttc cgccgccatt ttgagcgtaa ttcagagtac tacttttttg
     4801 ctcaccctta gaatagcttt aaatattaat ttttagagta tcataaccta cccgtaaaga
     4861 agatttcaaa atgttgaaag tcgcaaaatc atcatttaac ccgaatttaa agggcgataa
     4921 attgcggtat aacttttgaa caacttgtcc tgctccattt aaaggatacc ttgagaagtg
     4981 taacttttaa gcaactccat cgtcttacat gaaatttaca gccatagatg accgttgagg
     5041 attttaacag ctgcgtagat ttatactccg acggaatata tcgctttgca ttgaaaatgc
     5101 ttggcgacac catgctggca gaggataacg tgcaggattc tttcgagagg ctatgggtta
     5161 agcgtggtga aattgatagg tcaaaagcaa agtcatacct tttcaccact tcgtaccatg
     5221 cctcggttga cgccatcagg agggataggc acaagcagga gctagaaaat gcgcagccag
     5281 tattagaaat tggctatcaa cccaattttg acgtaaagga aattatcaac catgcggtgg
     5341 accagcttcc aacggttcaa aaatccgtgc tgctgctccg cgactacgag gggtactcct
     5401 acggtgaaat tgcagggata acaggactga acgaatcgca ggttaaggtt tacatctaca
     5461 gagccagagt tttcctaaag gaaaaactcg taagtattga aaatttaatt taactccgat
     5521 gagcatcaac ctaaatacat acgaggagta cctgctcgac tacatcgatg gcaagctttg
     5581 cccggaggtt gaggctgaac tagtaaagtt tcttcgctta catcccgaca ttgcttcaga
     5641 acttgatgga ttaaaccagc taaccattcc aaaaccaaac ctttcctttt cagggaaaca
     5701 acggcttagg agaaccgttg aacaggatct cttctcatgc aacgagatgg actacctttg
     5761 catcgccgaa ctggagggtg atatttccga ggttgaaaaa gcaaggctcg aagagctgaa
     5821 agggcagcat cccgaggttg aacggttccg caacctttat gcccataccc ttttactttc
     5881 cgagaatagc catacttttc caaacaaagc ttcgctaaaa aggcatagta tcatttcaaa
     5941 acgaaggatt atgctaacag cggcagcagc gattactgca gccattgttg gccttcatac
     6001 catccacaag ctaagccttc ccgacgattt gcaaccccaa aatcaaatgg ccaaaagcgc
     6061 aatcattgaa tcgcagccaa gggagtacag gccaacctcc accaccactt acccacaaac
     6121 ccagccaaaa ccggagagcc gggtagcagt gtcgctggca tataaaacct ctacatcaac
     6181 aataagcatg gacaacgaag agcttcagcc catgagaaca aatgtggcag agctaaagca
     6241 aatagcctcc aaagagccga taattgagcc aattgctagt attaccccta aaccctttcc
     6301 aataactggc aaggtacaag gtgttgcttt tcgggaaaaa gaattagcac ctaatgtagc
     6361 cccatcaact gccaaagttt acacgctggc cgatattgct ggcctgagtt taaaacgtct
     6421 tgcagggttg gttggcatca gctataacgt aaaaaccgac agcaccaaca cctccaagca
     6481 tattgaggtt gactccaggc taatagccat ctccaccata attcgaaaaa ataactagcg
     6541 gtgctgtaac aaaacgacct atcctaccgt cctacagaaa attttaatca accttctaac
     6601 ttcaactaaa atgaaacagc tagtaattct tgcatcatcg ctgctgagct ttttcaccct
     6661 aaccgcacaa aacgaaaagg aaaccatgct ccgcagcctc gaaatcgctg acactttaaa
     6721 agtctctgat accctcagca caaatcatag gaaacccaac atggtggtta atgtggagaa
     6781 aaccattaaa gtagctgatg gcgatacggt tgaggagact acctccgaca cacttgttgt
     6841 tgatagcaat accggggaca ataatgttgc ttaccaaggc gacacgataa tagtggacag
     6901 aaaccaaggc aacacgaaat cggactctac cataatcagg cttggcgaaa aaaccatcgt
     6961 gattgttgag ggtaaggata aaactaccgt tgaaatcccc gagggtctga acaaggattt
     7021 taggaactcg ttcaggcgta gcaaccgact aaaatttaag ggtcattggg ctggaattga
     7081 aatgggtgta aatggcttta tggatgccga ccactccacc tcgatgagca acgagcttga
     7141 atggctcgac ctgaagcaaa gccgttcgtg gaacgtaaac ctaaattttc tacagtacag
     7201 catgggtttt ggaaccagct ggattggatt ggtaaccggt atggggcttg aatttaacga
     7261 ctaccatttt aataacccct tgactctaaa ggttgtaaac ggagtaacgg ttgccgacag
     7321 ctcctacctg cttgccggat acaaggttga taaaacaaaa ctaagcacag ttaacctgac
     7381 ccttcctttg ctagtagagt ttcagatacc cactagcgaa tccgatcgcc acaaaattta
     7441 tatttcggcg ggagtaattg gtgggttgag gattggcacc cacaccaagg tagttttcga
     7501 cgatggcgat cgccataagg ataaggggcg tagcgacttc aacattgcaa ccttccgtta
     7561 cggcgttact gccagagcag gatacaggag tttacggctt tttgccaact actacccaac
     7621 ccaacttttt gaaaagaaca aaggcccgga aatttacccg tttgcggtag gactggtgct
     7681 tataccattc tagtgtaagc tgatagaata tagaatgccc atcgtaatac caaacgtttc
     7741 ggacagcgta taaaggttgg gctaaagtgg ctgcggccgg ggctttgccc cggccgcttt
     7801 ccttttacca cctcaaactc ttcctccctt ttcccattac atcttactta caggaaaagt
     7861 aaggctgaaa aaatacagca gatgcttctg tttttccatt ccagcattta atccggtaaa
     7921 cgtcaacttc atgattaggt tgattgggtt gaataaactg tctctttcaa taaggcatta
     7981 tcatgaacag gctaaatgca gctccttgca gatgcgtaag aattcaacta acctcccctt
     8041 ctaacttaaa gctgccgctc ctcctccacg aactttaccc cgtactcctc aagctcattc
     8101 agaatggggt tgtagaggtg gggtttaatg ggcatcacca cacccctgtc attcacctgc
     8161 ccggtcatta gcattttggc agctatggcc aggggtgtgc ccaccgtttt ggccatggcg
     8221 gtatgaacgg tatcctcgcc aacgagcgta agagttgatt tcttctgaaa cttcttcccg
     8281 tcaagctcat agtcgaatat atgctgcatg atgaggatat ccttatcgtt gggttcaaga
     8341 ccaagttttt cctccataat tctctgcagc agctgggcgg ggcttgcgtt ggccatgcca
     8401 ataagctcgt tgtcaaaaat gcccagccat tttagcttgt taaaaataat cttatcctga
     8461 gcagcaggaa tcacccgctg cagtttctcc tcaacggttt cggtaggatg atagggtaga
     8521 aaactgttga gaaactccct tttggtcaac atctcgctgc cctccatctt gtaggtatcg
     8581 tcggtcattc ccagctggac aaacacatcc caggctttgc tgtagcccga ccggcgtagc
     8641 gttccgcgta taatggtttg aatatcgtta ataccgtata tttccctgta cttaagagag
     8701 tcgcgatttg ggtacatttc aaactcgcca taggggggaa tgttgtaggt gcgaatgttg
     8761 gagtaaagcc tgtggtatgg aatgtactta tgctctccat tctcaaggaa atgcgtaacc
     8821 ccttgtcctg caagcactac gttgcgtggg ttccaggtaa acttgtagtt ccatgggttg
     8881 ttgtcgcact caggagcaat caaccctccg gtgttgctga tgaatccggt aattttccct
     8941 cccttgttcc taataccgtc aatcatcctc atggccgaca tgtggtcaat tccggggtca
     9001 acccccagct cgttcatcaa ggcaacccct ttttgtctgg cctcatcgtg gagcgatttc
     9061 atggcatcgg aaacgtacga tgcggttagc aggtgcttcc cctgtcggac gcactcctta
     9121 gccaccactg ggtgcatgaa tgcaggcagc atggatattg ccacatcggc ccaggccacc
     9181 tcagcgctga gctgttcagc attaaaaata tcgaacttga aggcctgtcc atttggattt
     9241 cccccaagct taccggcggc tgcttccaac gaaagatcac caatacgaat tgtccagcca
     9301 taggctggcg cattccccaa taggtactca ataaggttgg tggacgaaag cccggcaccc
     9361 agtatcaaaa ttctactcat ctgcatggct tgtatgttgt tttagttact atttactaaa
     9421 ccatttatca cctagcattc aaacgatcaa gctacagacc taaccggtcc gctgcccttg
     9481 tatgcaaacc ggctgatgct aaaatagtaa aaccagcccc gcttgctttc agctttaaag
     9541 tttaccgcta aatatcctgc caatctaaaa cattttttgg gaatatttga attatttttt
     9601 taaaattcac accgtttgtt aataacaaga tttcctcaac tttatacatg aatagattac
     9661 tgtataatca tttcagtcag taagaaatta ccatagagca aggtttttta catgttctaa
     9721 atcgctatct ttgaccaaat ggtgccgaaa tgcttaccct gtcgattggt tttggcgagc
     9781 ttgtggttat cctgctggtt accctgctgg tggttggccc tcgtgaggta gcaaaggttg
     9841 ccaggaagct gggaaaactt taccgaagga tggataacgc catgtccgat ttacgcagct
     9901 acgtggaaaa tccctccagc ccgggtgata acagcgattt ggttgataca agtgaaaaaa
     9961 ggaacgattc ggataatgca aacaaaacac cggcaggata agtgccacca caacgttact
    10021 ttgggctaaa ttggtacatt tgcgcaggca aaaatctaat ggatagcaat gataaaggtc
    10081 gaaaatatta ccaagtccta cggtgcactg atggttctaa aaggtatcac ccttgaaata
    10141 cctgagaaaa aagttgtagc aattgttggc cctagtggcg ccggtaaaac aaccctacta
    10201 caaataatgg gtaccctggg cagagctgat tccggacaag tgcttattaa tggcgaaaat
    10261 gtagcacagc tgaacgaccg ccagctggcc aaatttagga atacccatat tggttttgtt
    10321 ttccagttcc accatctttt acccgaattc actgccttag aaaacgtttg tatgcccgcc
    10381 ctgattgcag gaaaaggtat ggcaacggcc acaaaaaagt ccaccgagct gctcaagatg
    10441 cttggcctat cggacaggct cgaccataag ccctcggagc tgtcgggcgg cgagcagcaa
    10501 cgcgtggccg tggccagagc actggttaac aatcctacgg taattctggc cgatgaaccc
    10561 tccggtaacc tggactcccg aaataaggag gagctgcaca aacttttctt catgcttcgc
    10621 gaccagctgg gccaaacctt tgtggtggtt actcacgata aggatcttgc ccaaatggct
    10681 gacctgcagg ttggcatgaa cgacggactg ctttgcccat gaaacttcca caaaaccgtg
    10741 cagagctgac acaactactc aacaaccgct atcatcgcta tgccggagca tccttccttt
    10801 ccgacgaccc tatacttata ccccaccgtt ttactaaaac cgaggacatt gagatatctg
    10861 ccctcctcac cgccaccatc gcatggggca cccgtaaaag cataataggc agcatgaaca
    10921 ggcttatggc cataatggac aacagcccgt tcgagttcgt gataaatttc tcagaaaacg
    10981 atgagaaacg gctaaacggc tttgcccatc gaaccttcaa ctcatccgac tgcattgcgt
    11041 ttattagaag tttgcagcgc atctacgccg atggtggagg cttaaaagcc atctttacca
    11101 atggcttccg gcaaacaaga acagccaagc aagcgttgga ggcattccgg ctccggttaa
    11161 ttcaggaaaa ttttagcggt catgccatga agcatgttcc aaacgtggag tcgggttccg
    11221 cagccaaacg cctcaacatg ttgagacggg taattaaggt agcgtaggaa acccttgtca
    11281 ttattagtat ttttacctgt gtaaagatat atgtatggga caagacttga aagaactgaa
    11341 ggattttctt gatgaaatgg ttacacaata taacaatccg acatttattg gacttgaccc
    11401 aatacaaata ccgcattcat acaccataaa ggaagacatc gaaatatcag gatttcttgc
    11461 agcaataata tcatggggaa atcggacttc catcgtgaaa aatgcaaaac ggatgctggc
    11521 gtacatggga gattcaccat atgacttcat tctcaaccac agagagcaac atctgaatca
    11581 gatggaaggt tttgtgcatc gtacatttaa ttcagttgac ctgttgacat tcattgaagg
    11641 tctgaagtac atatataagg ataaaaatgg actggaaggg atatttgtga aatatcaggc
    11701 taatgattca ctgcaacctg caatacatga atttaaaaaa gaattcttta acatacctca
    11761 tcttgagaga acccgaaaac acctgcctga cccgatgact ggttctgctg cgaaaaaaat
    11821 aaatatgtac ctcagatgga tgattcgcca agataatgca ggtgtagatt ttggcatttg
    11881 gaaaaatata tcaccttcaa aattatcatg tccacttgat acccattcag gaaacgttgc
    11941 ccgcaaactt ggtttgcttg gtcgaaaaca gaatgatgca aaagcagtac aggaattgga
    12001 tggagttctg cgacaacttg actgtcatga ccctgtgaaa tacgatttcg cattgttcgg
    12061 attaggtgtt tcaaatgact tgaaaatcat ctaatacact cttttacaaa aaatgagtta
    12121 tttcaaggaa cttaatagga aaaggtttag aagcaaaaaa tatggaaaaa ttaatacttg
    12181 aaacattcta ctttatatac ccagcaatac ttttatagtt cacataaaaa ttttcaaaaa
    12241 aatttcaacc atcaaatatt caacgtttta aaaatttcat ctggaaattt ttttgacatc
    12301 aaatttttgc tttgcaatta atcgtattgg atttcgaaat tcttgatgta gcacccacat
    12361 ccatagagca ccccccaccc agcagggggt caaaaagaag gtatttcccc cccccttctc
    12421 ccaacaaatg attcccattt tcga
//
LOCUS       DAOPHX010000077        12133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_6243_length_12133_cov_6.224458, whole genome shotgun sequence.
ACCESSION   DAOPHX010000077 DAOPHX010000000
VERSION     DAOPHX010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 12133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12133
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6243_length_12133_cov_6.224458"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..1799)
                     /locus_tag="PK502_06905"
     CDS             complement(189..1799)
                     /locus_tag="PK502_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HPH00582.1"
                     /translation="MKQPKKKREFPHTYVIIFTLIVVAALLTWFIPGGEFTREVKSIN
                     GIDREVIVPGSFHQVDNSPQTWQVFTSIFQGMRRTCDIIFYILIIGGSFWMLNESKAL
                     DVAILSFLNFTRRIERFKPLKAIGVNNLVIALIMICFSFFGAVIGMSEETIAFVVIFV
                     PLAISMGYDSIVGICLCFLGAGLGFASALLNPFTIGIAQGLSGIPLFSGIEYRFIIWL
                     VINTIGISYVLWYANRIRKNPKLSPVYEIDEHWRHKASEEGAIVKTKAGKIAWVVFAV
                     VAIVLAIFAYLYPTSQLVVGQNVISVPAIPIATVVWVILGLLALRHSVQLFIVNILMF
                     TILFLIVGVMGYGWYIKEIASLFLVMGILASAAFGKNANQLTRSFIDGARDIMSAALV
                     VGLASGIVVVLEQGKVIDSLLNYSANAMQGYGKVTSMAIMYIFYNLLNLIIASGSAKA
                     ALTIPLMAQFSDLIGISRQATVTVYQLGGGFTNLITPTSGVMVGVLSIARIPYNKWFR
                     WFLPLMFILIVAGFLLLLPTLFLPLSGF"
     gene            2039..2875
                     /locus_tag="PK502_06910"
     CDS             2039..2875
                     /locus_tag="PK502_06910"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPH00583.1"
                     /translation="MKDRQLDIAVISDLHLATYACKPKKILKYLKSIKPQMLVLNGDI
                     IDSWRFSRNYFPKSHLKVVRQIIKMMEKGVKVYYITGNHDEFLRKFSPVEAGNLKIVD
                     RLVLDQDGGKTLILHGDIFDHSIYMTKWLAKLGAAGKGMLSLIDAFINGLLGIFGRKD
                     FILYKTINQKLNRERSTLIGYEKSMLKSCAEQGYQTVICGHTHFPKDRELLINNKRVH
                     YLNCGDWVEHFTAAEYYNGSWHLYVQNETGDELLYDEPEIPAGRQLYQKVKLELAFSN
                     LG"
     gene            complement(2949..3022)
                     /locus_tag="PK502_06915"
     tRNA            complement(2949..3022)
                     /locus_tag="PK502_06915"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2986..2988),aa:Pro,seq:ggg)
     gene            3351..5834
                     /gene="priA"
                     /locus_tag="PK502_06920"
     CDS             3351..5834
                     /gene="priA"
                     /locus_tag="PK502_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPH00584.1"
                     /translation="MAQAGDCFVDVVLPLALPRLYTYSIPDEMIGRVEQGMRVVVSFG
                     RKKLYAAVVFRIHSDAPNGYQTKDIIQLIDEQPVVLPGQLKFWSWVADYYMCYLGEVM
                     KAALPSGLKMESESFVSQANVEDINFSDSERIILHLLDGKKKRRIQDIATMAGFKNPM
                     GAIKSLLEKNAIFISEEIEEAYRPKTEKYLSISPKYCSNDAINELLNSLGRAPAQQRL
                     LLGMLSLTDNFSELSDVCVPKRKVLEKVDVSAAVVVSLVAKGVICEVEKAVSRVNDAD
                     NQLGKPVELSSAQLEALNRVKESFATHHVTLLHGVTSSGKTEVYIHLIGEALAEGKQV
                     LYLLPEIALTTQIISRLRRIFGDRVGVYHSKFSDSQRVEVYNGVLRAESTPGKPAYSV
                     ILGVRSSVFLPFRNLGLVIVDEEHENTYKQFNPAPRYQARDAAIMLAGFHGAKVLLGT
                     ATPSVETYFNARTGKYGLVELADRYLNMELPQVEVVDVREARKRKLMRSHFSEKLIEE
                     IGLSLERNEQVILFQNRRGFAPFIECHECGWVPHCENCDVTLTYHKSSDLLVCHYCGY
                     SVKPTHECMACGSTNLQPQGFGTEKVEDELQIFFPEASIERIDLDSTRSRTAYEGIIA
                     RFETGQTDILIGTQMVTKGLDFDRVSLVGVLNADNMLNFPDFRASERSFQLMAQVSGR
                     AGRKNRRGLVIIQTSQPNHPVIKQVKENDYMAFYATVVGERQKFLYPPFCRLISISIK
                     HKDQNVARQASEVMANGLKHSLGNRVLGPEAPMVGKIQGYYIQSILVKAGGQQSLSRV
                     KELIAYMANSLRVNGEYRSLVVAVDVDPQ"
     gene            complement(5849..6556)
                     /locus_tag="PK502_06925"
     CDS             complement(5849..6556)
                     /locus_tag="PK502_06925"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HPH00585.1"
                     /translation="MEKFKSDLSGQEFPISERVSGKSIRQPIMELIQKDFPQFSAEEF
                     LSLTELNDYREKYITEYLVRQTGELTELEQKVIDSISDRATLSDKLVEDEAQRTFGQK
                     IADKVAAFGGSWTFIIAFLGFILIWICLNVFWLANRNFDPFPFILLNLILSCIAAIQA
                     PVIMMSQNRQESKDRERAKKDYMINLKAELEIRSLHEKIDHLILRQQQEIVEMQKVQT
                     DMLSDIAKEVVSSSERE"
     gene            complement(6642..7865)
                     /gene="hydF"
                     /locus_tag="PK502_06930"
     CDS             complement(6642..7865)
                     /gene="hydF"
                     /locus_tag="PK502_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HPH00586.1"
                     /translation="MQRGKESKPHIGIYGRRNNGKSSLINLLAGQNVAIVSDFAGTTT
                     DPVKKSFEITGFGPVILVDTAGIDDSGELGEKRVGKTLETINQVDLAILVVAGNTWGD
                     YEERLIEEFSTSDTPFLVIHNKSDLTPASEEFKRVVGSKPHRGFAEISCLWPNNVDTL
                     VELIKGAIPESSYQVPTLLGDLISYGDVVLLITPIDVEAPAGRLILPQVQAIRDVLDN
                     DAVAIVLKEREVDDFLRKTRIKPALAVTDSQIFSKADASIPRDIPLTSFSILLARMKG
                     DFASYLKGTPKISQLKNGDRVLLLESCSHHVSCDDIGRTKIPRWLSNFTGKQLEYDVV
                     AGLSSLPRSITDYALVIQCGGCMITRKQLINRIRPAVEAGIPVTNYGMAIAYVQGIYQ
                     RAIAPFAPASPTSYL"
     gene            8050..8973
                     /locus_tag="PK502_06935"
     CDS             8050..8973
                     /locus_tag="PK502_06935"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HPH00587.1"
                     /translation="MNKLTTTIIMVSFMITAFSGESKSQVGGMLKDRLKKAAVKSVLS
                     NQDEGQSDTISEKQQNQQAENPTETYMQAKMMGLMGKGNVKYDLSYTFTSSMKMDFEV
                     LDSAQNEPFTGTYTLYFEKDGKNFAMEVETAGKKDEPASKSLFIYDFRNLALLILSEQ
                     EGQNSGMAMAIPPDSSKVEPSQVAPTTDTELPGNEVSTGDVTFRATGRTKSILGYSCK
                     EYLMENPEGKVELWVTNDVRYDYSDAYNRMQGFQMMSNVNSSATNGTVLEMHFKDSGS
                     PAHSDFYVREINKDKSQTFNLAGYTIMSFGQ"
     gene            9822..10358
                     /locus_tag="PK502_06940"
     CDS             9822..10358
                     /locus_tag="PK502_06940"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HPH00588.1"
                     /translation="MFAGGKSSRMGKRLLPVNGKPLIQHIAEQLVDFLDEAIIGANDT
                     EKYGFLNLRVVPDMEMEKGPLMGIYSCLLASDSDINFITACDIPEMNIELVSRMANLA
                     LDYEIVMPVNGSSRFEPLFAVYRKSVAAKAKLLLDGNRQRIVELLDCTEYATVDFTGN
                     GWYQNLNCASDYFEYVGR"
     gene            complement(10443..>12133)
                     /locus_tag="PK502_06945"
     CDS             complement(10443..>12133)
                     /locus_tag="PK502_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00589.1"
                     /translation="QVDGRCETLSLSYRGDELYLFRDDNHDGNIYVSHLENGRWTPIR
                     KLNPNINSEYYETHACLSPDGKDLYLSSNRKGGFGDLDIYVSHRESGDNWEVPQNLGS
                     TINTKLNENTPFLTSDGLSLYFSSEGHSNMGGYDIFVSQLEADGRWTAPINLGYPINT
                     TDDDLFFFPIDDGSSALMAMFDPEGMGSQDIYELKLFVPRFMRGIVSTTEVSDRISDK
                     TFNRLVVDTISFRGKALVDLTSSDIPQIIDPKLKSKLVFDGRKFDISQKISYTQTRLP
                     KAEQGENIGFPLTAARARPNLSQPFKTFADTGMGISKLIGDTNKLISGYTKPDRIRGR
                     SSQLTPDGYAGTNVETYSYLMETLLLLTPPNLQSVLSPILKNNWQFPDNTLSESILNF
                     SSAFKTYEGRDAMVRALANLSDVISDKTSGNTSQRRQTIASDSEYGSFTYFYNQLINL
                     SSPNLAKQLAAITVGHPKINTFSALLAAYKKANPKGYRENLPELLRVFSRISISAYNA
                     LPDEDKINLYSDITQDVTSGKRNVWYFYLLLFLVVALAGYLLFRYLKLRKESQS"
BASE COUNT         3216 a         2665 c         2998 g         3254 t
ORIGIN      
        1 taatttggaa ccctgacgat tacaagtaag cgttgttcag gatatgaaca tcaagcggaa
       61 cttttaaaca gttggtgcaa ccaaatattg ctccggggat tttggatttt acggtgctgg
      121 tataggcgtt cctgcagcgc gatatgtcat tttccagagt ttcctaccga ttaagtcaaa
      181 ggttggcatt aaaacccgct cagggggagg aaaagcgtag gcagcagcag caggaacccg
      241 gctacaatca ggatgaacat tagcggaagg aaccacctga accacttgtt gtagggtatt
      301 cgggcaatgc tcagcacgcc aaccattacc cccgatgtgg gggtgataag gttggtaaaa
      361 ccgccgccca gctggtaaac cgttaccgtg gcctgtctgc ttatgccgat gaggtcggag
      421 aattgtgcca tcagcgggat ggtgagggca gccttagccg atccggaggc aataatcagg
      481 ttgagcaggt tgtagaagat gtacatgatg gccatggagg ttaccttccc gtaaccctgc
      541 atggcgttgg ccgagtagtt cagcagcgaa tctataacct ttccttgctc cagcaccacc
      601 acaatcccac tggccagccc aacaaccagc gcagccgaca tgatatccct tgccccatca
      661 atgaacgagc gtgtaagctg gttggcgttc ttaccaaatg cagctgaggc cagaataccc
      721 atgaccagga atagcgatgc tatttccttg atgtaccatc catagcccat aacgccaaca
      781 atgaggaaca ggatggtgaa catgaggatg tttacaatga acagctgcac ggagtgcctt
      841 aatgccagaa gccctagtat gacccacact acggtggcta tgggaatagc cggaaccgaa
      901 atgacatttt ggccgactac cagctggctg gtggggtaga ggtaggcgaa aatggccagc
      961 acaatggcta ccacggcaaa gacaacccat gcgattttgc cggctttggt tttaacgatt
     1021 gccccctctt ctgaagcctt gtggcgccag tgctcgtcaa tctcgtaaac tggagatagc
     1081 ttggggttct tcctgatgcg gttggcatac catagcacgt agctaatacc tatggtgttg
     1141 atcaccagcc agattatgaa tcggtactca atccccgaaa acagcggtat tcccgaaagt
     1201 ccctgggcaa ttcctatggt aaaggggttt agcagtgcgc tggcaaaacc cagacccgca
     1261 cccagaaagc acaggcatat tccaacaatg gagtcgtagc ccatgcttat ggccagcggc
     1321 acaaaaatta ccacaaaggc gatggtctcc tcgctcatcc ctattaccgc cccaaaaaag
     1381 ctgaagcaga tcatgatgag cgctatcacc aggttattca ccccgattgc cttcagtggc
     1441 ttgaacctct ctattcgccg ggtaaagttc agaaacgaca ggatggccac atccaacgct
     1501 ttactctcgt ttagcatcca aaacgaaccg ccgattatca gaatgtagaa gataatgtcg
     1561 caggtacgcc tcatgccctg aaaaatggag gtgaacacct gccatgtttg cgggctgttg
     1621 tcaacctggt gaaaggagcc gggaacaatc acttcgcgat caatcccgtt gatactcttc
     1681 acctcccggg tgaactcccc tccggggata aaccaggtga gaagggctgc cacaactata
     1741 agcgtgaaga tgatgacgta ggtatgcgga aactcgcgtt tcttcttagg ttgtttcatt
     1801 gcgcctattt ttgaatggct aaaatagttt tttttcatat tcatctatcg tcggtggtgg
     1861 caaccatcca tttttattct ttttataatg gcaaaatgcg ccctgcatgg ttaatgctac
     1921 cctatagtaa ttgtgcggtg taaagtggta acaaaggatt attcaaatcc taacgtttta
     1981 gtaacatgta ttttacaact aaactgctcc tttgcataaa atttatacct gtagctaaat
     2041 gaaggataga cagttggaca tagccgtgat ttccgatctg cacctggcta cctatgcctg
     2101 caaaccaaaa aaaattctga agtaccttaa atctattaag ccgcagatgc ttgtcctcaa
     2161 cggtgatatt attgattcgt ggcgcttcag cagaaattac tttcccaaaa gccacctgaa
     2221 ggttgtaagg cagattatca agatgatgga gaagggggtt aaggtttact acattacggg
     2281 gaaccacgat gagttcctta ggaagtttag ccctgttgaa gcaggaaatc tcaaaattgt
     2341 tgatcggctt gttttggatc aggatggagg gaaaacccta atcctccatg gcgatatttt
     2401 cgaccatagc atatacatga caaaatggtt ggcaaaactt ggtgctgctg ggaaagggat
     2461 gctatcgttg attgatgctt ttataaatgg gcttcttgga atattcggga gaaaggactt
     2521 cattttgtac aaaacgataa accaaaagct aaacagggag agaagtaccc tcataggcta
     2581 tgaaaagtct atgctaaaat catgtgctga gcaaggctac caaacggtaa tttgcgggca
     2641 tacccacttt cctaaggata gggagctgct tataaacaac aagagggttc actacctgaa
     2701 ctgtggcgat tgggtggagc acttcactgc agccgagtac tacaatggtt cctggcatct
     2761 ttacgttcaa aacgaaaccg gggatgagct gctatatgat gagccggaaa taccagcagg
     2821 tcgccagctg taccagaagg ttaaactcga gttggcattt tctaacctgg ggtagctcgc
     2881 cgggtaaatt ttaaatagcc gggtattgaa attaaaaagg caaccaccgg ttgcctttta
     2941 ctgttttgtc ggggtgatcc gactcgaacg gacgaccact tgcaccccat gcaagtgcgc
     3001 tagccaactg cgccacaccc cgaagcaaaa tttcgccctg cttcataacc gaaaatggtt
     3061 gaaaaaatta gccatctctc gaatttctga gcaaagcgtt gcaaaaatac acccttttta
     3121 gtttgtttca aaaattttta ttcaatcctg caaagatttt cataccttgc ccgtatgttt
     3181 tactttagga agccagctct cccggctagg cgaagggctt gtgtaacaaa cccagctgca
     3241 ccttgcagat ggtttctact tgcatatttt cctttacaca cgagttttta atgatgcagg
     3301 gctcattgtt tttttttaca tcaggtaatg gctaattgga gtggcaggta atggctcagg
     3361 caggagattg ctttgttgat gttgtgcttc cactagcgct tccaaggctc tacacctact
     3421 ccattcccga tgaaatgata ggtagggttg aacagggaat gcgggttgtg gtttcgtttg
     3481 gacgaaagaa actttacgct gcggttgtgt tcaggattca tagtgatgcc cccaatgggt
     3541 atcaaaccaa ggacatcatc cagcttattg acgagcagcc ggttgtacta cccggccagc
     3601 tgaagttttg gagttgggtg gccgactact acatgtgcta tttgggtgag gtgatgaagg
     3661 ctgcacttcc ctccggcctg aagatggaga gcgaaagctt tgtgagccaa gcgaatgttg
     3721 aagatattaa cttttcggat agcgaacgga ttatcctgca cctgcttgac ggtaagaaga
     3781 agcgtagaat acaggatatt gccaccatgg caggttttaa aaatcctatg ggggcgatta
     3841 agtcgctgtt ggagaaaaat gcaatcttca tctccgagga gattgaggag gcgtacagac
     3901 caaaaacgga gaagtacttg tcaatttcac caaaatattg tagcaacgat gctatcaacg
     3961 agttgcttaa tagccttggc agggctccgg ctcagcagag gttgctgctt gggatgcttt
     4021 cgttgaccga taatttttcc gaactgtccg atgtttgtgt gccgaaacgc aaagtcctcg
     4081 aaaaagttga tgtctcagca gctgttgttg ttagtcttgt tgccaaagga gtaatttgtg
     4141 aggttgaaaa ggctgtttcg cgggttaacg atgccgacaa ccagctggga aagccggtgg
     4201 agttgagcag tgctcagctt gaagctttga atagagttaa ggaatcgttt gccacccacc
     4261 atgtaaccct gcttcatggt gtaacctcta gcggaaaaac tgaggtgtac attcatttaa
     4321 ttggcgaagc gcttgcagaa ggtaaacagg tgctgtacct gctacccgag attgcactta
     4381 ccacacagat aatcagcagg ttgagaagaa tatttggcga tagggtaggg gtgtaccact
     4441 ccaagttcag cgattcgcag cgggttgagg tgtacaacgg agtgctgcgt gccgaaagta
     4501 ctccgggaaa acctgcctac agcgtgattc ttggtgttcg ctcatcggtt ttcttaccct
     4561 tcaggaatct tgggttggtg attgttgacg aggagcatga aaacacctat aagcagttta
     4621 accccgctcc gcgataccag gcccgtgatg ccgccattat gctggccggg tttcatggcg
     4681 caaaggtact gctcgggaca gccacaccat cggttgaaac ctactttaac gcccgaacgg
     4741 gtaagtatgg tttggtggag cttgctgacc gctacctaaa catggagctg ccccaagtgg
     4801 aggtggttga tgtacgggag gcccgaaagc gcaagctgat gcgctcgcac ttctccgaaa
     4861 agcttattga ggaaattggg ctatcccttg aacgcaatga gcaggtcatt ctgtttcaga
     4921 acaggcgagg ctttgcgcca tttatcgagt gtcatgagtg cggctgggtt ccccactgcg
     4981 agaactgcga cgtaacccta acctaccata agtcgtcgga cctgctggtg tgccactact
     5041 gcggctactc tgttaaacct acccatgagt gcatggcatg tggtagcacc aacctgcaac
     5101 cgcagggatt tggaaccgag aaggtggagg atgagctgca aatatttttc cccgaagctt
     5161 caattgaacg catagatctt gactcaaccc gttcacgaac tgcctacgag ggaattattg
     5221 cccggtttga gacggggcaa accgatatac tcatcggaac acaaatggtt acaaagggtt
     5281 tggatttcga cagggttagc ctggtgggtg tactaaatgc cgacaacatg cttaattttc
     5341 ccgatttcag ggcatccgaa aggagttttc agctgatggc gcaggtaagt ggacgtgccg
     5401 ggcgtaagaa ccgacggggc ttggtaatca ttcaaacatc gcaacctaac catccggtga
     5461 ttaagcaggt gaaggaaaac gactacatgg ctttttacgc cactgtagtt ggggagcgac
     5521 agaagttcct ctacccacca ttttgccggt taatttccat ttcgattaaa cataaggacc
     5581 aaaatgttgc ccgacaggct tccgaagtaa tggcaaatgg gttgaagcat agcttgggta
     5641 acagggtgct tgggccagaa gccccgatgg ttggaaaaat tcagggttat tacattcaaa
     5701 gcattctggt taaggctggc gggcagcaat ccctttcgag ggttaaggag ctaattgcct
     5761 acatggccaa cagcctgagg gtaaatggcg agtaccgttc gttggttgtt gcggttgatg
     5821 ttgatcccca gtagggaggg ctatctcttt actccctttc cgagctactt accacctctt
     5881 tggcaatgtc gctgagcata tcagtttgaa ccttctgcat ctcaactatt tcttgctgct
     5941 ggcgtaaaat caggtgatca attttttcgt gaagcgacct gatttccagc tcagccttaa
     6001 ggtttatcat gtagtccttt ttagcccttt cccgatcctt agactcctgg cggttttggc
     6061 tcatcataat aacaggtgcc tgaatggcag caatgcacga tagaataagg ttgagcagga
     6121 tgaatggaaa agggtcgaag ttccggttgg ccagccagaa aacattcagg caaatccaga
     6181 ttaaaataaa acccagaaaa gcaataatga aggtccagct accaccgaat gcggcaacct
     6241 tgtcggcaat tttctggccg aaagtacgtt gagcttcatc ctcaaccagc ttatccgaaa
     6301 gggttgcccg gtcggatatg gagtctatca ctttttgctc aagctcggtg agctcgccgg
     6361 tttgcctcac caggtattcg gtgatgtact tctcgcggta atcgttaagc tctgttagcg
     6421 ataggaactc ttcggctgaa aactgaggga aatccttttg aatcagctcc atgatcggct
     6481 ggcggattga tttacctgaa acccgttcag aaattggaaa ttcctgaccc gataggtcac
     6541 ttttgaattt ttccataacc tttcattaat ttgttcgtac ctgaaggaga tttgaagttt
     6601 cggactgcta tattaagctg gggtaaaacg ggtaaactca actataggta gctggttgga
     6661 ctagccggag caaagggcgc aatggcacgc tggtatattc cctgaacata ggcaatggcc
     6721 ataccgtagt tggtaactgg tattcccgct tcaacggcag gtcttatcct gttgataagc
     6781 tgcttgcggg taatcatgca gccgccacac tgaataacca gtgcgtaatc ggtgatgctg
     6841 cggggtaggg agctaagccc tgccaccacg tcgtactcca gctgtttacc ggtgaagttg
     6901 gaaagccacc tggggatttt tgtacggcca atatcgtcgc aggaaacgtg gtggctacag
     6961 ctttcgagaa gcaacaccct gtcgccattc tttagctgcg aaatttttgg ggttcccttc
     7021 aggtagctgg caaaatcacc cttcatcctt gcaagcagta tgctgaagct ggtaagggga
     7081 atatccctcg ggatggaggc atcggccttg gagaaaatct ggctatcggt aacagccagc
     7141 gcaggtttga tacgggtttt tctgaggaag tcgtcaacct cacgctcctt cagcactatg
     7201 gccaccgcat cgttatcaag cacatccctg atggcctgaa cctgtggtag tataagcctt
     7261 ccggctggag cctcaacgtc aatcggagtg ataaggagca ccacgtcgcc gtaggatatt
     7321 aaatcaccca aaagggttgg aacctgatac gacgattcgg gaatagcccc ctttatcaac
     7381 tcaaccaacg tatccacgtt gttaggccaa aggcagctta tttccgcaaa gcccctgtgg
     7441 ggtttggaac ccacaacccg tttgaactcc tcactggcag gggttaaatc ggatttattg
     7501 tggatgacca aaaagggggt atcgctggtt gaaaactctt cgatcagcct ttcctcataa
     7561 tcaccccagg tgttacctgc cacaaccaga atagccaaat ctacctggtt gattgtttcc
     7621 aatgttttac caacccgttt ttcccccagc tcgccgctat cgtcaattcc ggcggtatca
     7681 accagaataa ccggtccaaa gcccgtaatc tcaaacgatt tcttcacagg gtcggttgtg
     7741 gttccggcaa aatcggatac tattgctaca ttctgacctg caagcaggtt gataagcgag
     7801 ctttttccgt tgttgcgtct tccataaatg ccaatgtgtg gctttgactc ttttcctctc
     7861 tgcatgggct ggaatgtttg ttggcaaatt taggcattcg tcgactattt attcgttcct
     7921 tgttaaacat tatgcacaag cttcatgtcg gtatcaagaa tcttaatatc aacattaaag
     7981 aatgaaaata atttgcattt aacaaaacta tcggctaaat tgcatgtaat tccttataaa
     8041 taacatacca tgaataagtt aactacaacc attataatgg tttcatttat gataaccgct
     8101 tttagcgggg aatcgaagtc gcaggtgggg ggaatgctta aggataggct taaaaaggct
     8161 gctgtaaaga gtgtgctatc caatcaggat gagggtcaaa gcgataccat tagtgaaaaa
     8221 cagcaaaacc agcaggccga aaacccaacc gaaacctaca tgcaggcaaa gatgatgggg
     8281 ttgatgggaa agggaaacgt gaagtacgat ttatcctaca ccttcacctc gagcatgaag
     8341 atggattttg aggtgctaga ttcagcgcag aatgagcctt ttacaggaac ctacaccctt
     8401 tactttgaaa aggatggcaa aaattttgcc atggaggttg agactgccgg caaaaaggat
     8461 gagccagcct caaaatcgct attcatctat gatttccgca atttggccct gctgattctt
     8521 agcgagcagg aagggcaaaa tagcggcatg gccatggcta ttccacccga ttcttctaag
     8581 gtagaacctt ctcaggtggc gcctaccacg gacactgagc tacctggcaa cgaagtttca
     8641 actggtgatg taacctttag ggcaaccgga cgaaccaaaa gcatccttgg ctacagctgt
     8701 aaggagtacc ttatggagaa ccccgagggt aaggttgaac tttgggtaac aaacgatgtg
     8761 cgctatgact actccgatgc ctataaccgg atgcagggat tccaaatgat gtcgaacgtg
     8821 aactcatcag caaccaatgg aacagtgctg gaaatgcact tcaaggattc tggttcacct
     8881 gcgcattccg acttttacgt cagggagata aacaaggaca agagccaaac atttaacctg
     8941 gccggctaca ccattatgag ctttgggcag taggctggtt gaggatttgg aggcacttgc
     9001 cgcattactt caaatgattg cggcatttgc catttacggc agtttattgc agggggagcg
     9061 ctgtaggaat atggagctag atgaatatct aacctgacgc aatggtcaag gttgtcattt
     9121 ctaccgttta gttgttatta ccggttggta atgctagaaa ggttttccag cttagctatc
     9181 caaccattaa aatggctgca tgtttttttt agaaagtcaa tccctatttt cccaacaatt
     9241 actgccccaa cgcttacttt atcgtattcc ggaataagtt ttattattct tttcgaaggt
     9301 gctgtttcgg ggttatcatt aattagctct ggattcacct tttcagcgag aagtttctgt
     9361 aagttactta tttctttgga gcgctcaaaa tattcaattt ttaattcttc aggttttgat
     9421 agtatgagtg tttcaaattc ctatagctga aggtagggta taaatctatt atcttgaata
     9481 tcatctgcaa atgctttttc gaggaattcg acccgttcat aggcatcatt aatttttttt
     9541 gactcattat attttggaaa atcttttggt tggcgcacga ttctattcct cagaatgttt
     9601 cggtagtggt ggaattaaat tcgattcgaa aagtccttga cacgggtctg ttcattgtaa
     9661 taagaaattt aggagaaaca aggggtaaaa aagggctgct caaaagtaat tagcctgccg
     9721 ataaaattat tggctttcaa aataactcct gggtttttga gccagggcgt atatttgtta
     9781 aagacggaac atagcatatt cgtcaaaaag caaccgcaat aatttttgcc ggaggcaaaa
     9841 gttcccgaat gggtaagagg ttgctccctg taaacggaaa acctctaatt cagcacatcg
     9901 cagagcagct ggttgatttt ctcgacgagg caattatcgg ggcaaacgat acggaaaagt
     9961 atggcttcct aaatctgagg gttgttcctg atatggaaat ggagaaaggc cctttaatgg
    10021 gaatttactc ctgtttgctg gcttcggaca gcgacataaa ctttattaca gcctgcgata
    10081 tcccggaaat gaatattgag ctagtaagta gaatggccaa cttggcgtta gattacgaga
    10141 tagtaatgcc tgtgaatggt tcatcacggt tcgaacccct ttttgccgta tatcgtaaaa
    10201 gcgttgctgc taaggccaaa ttgcttttgg atggaaacag gcaaaggatt gttgagcttc
    10261 ttgattgtac tgaatacgct actgttgatt ttactggcaa tggatggtat caaaacctaa
    10321 actgcgctag cgattatttt gagtacgtag gtagataatt ttttctggag gtggttgcgg
    10381 gcaacaccag ctatacagga gtatcaaaaa gttccaacca gggggaagaa ctccaatgcc
    10441 tcctacgatt gcgattcttt ccttagcttc aggtacctaa atagcaagta accagccaat
    10501 gccacaacca gaaatagcaa caggtagaag taccaaacat ttctctttcc tgaggttaca
    10561 tcctgtgtga tatcgctgta caggttgatt ttatcctcat caggcagtgc attgtaagcg
    10621 ctgattgaaa ttcgtgaaaa aactctaagc agctcgggga ggttctccct atagcctttt
    10681 gggttggctt ttttataggc ggcaagcagg gcagaaaagg tgttgatttt tggatggcca
    10741 acagtaatgg cagctagctg tttggccaga tttggggagg aaaggttgat gagctgattg
    10801 taaaagtagg tgaatgagcc gtactcggag tcggaagcaa ttgtttgtct tctttgggag
    10861 gtgttgccgg aggttttgtc agaaataaca tcgctgaggt tggccagtgc tctcaccatg
    10921 gcatctctcc cctcgtaggt tttaaatgcg ctggaaaaat ttaaaatgct ctcgctaagg
    10981 gtgttatccg ggaactgcca gttgtttttg agtatagggc ttagaacaga ctgcaaattt
    11041 ggaggagtta gtagtagcag cgtttccatt aagtagctgt aggtttcgac gttagtgccg
    11101 gcgtacccat caggggtaag ctggctactc ctacccctta tacggtcagg cttcgtgtag
    11161 ccagaaatca gtttatttgt gtcacctatc agcttagaaa tgcccatgcc cgtatcggca
    11221 aaggtcttaa agggttggct aaggtttggt cttgccctag cagcagttag cggaaaaccg
    11281 atattttctc cttgttccgc cttgggtaag cgtgtctggg tgtagctaat tttctggcta
    11341 atgtcgaatt tcctaccgtc gaagacaagc ttgcttttaa gtttggggtc aataatttgg
    11401 ggtatatcgg agctggtgag gtcaacaaga gctttacctc taaagctaat ggtgtcaacc
    11461 accaaccggt taaacgtttt atcggagatt ctgtcggata cctcggtagt tgacactatg
    11521 cctcgcatga atctgggaac aaacagtttc agctcgtaaa tatcctgaga gcccatcccc
    11581 tcggggtcaa acatggccat taatgcgctg gagccatcat caatcgggaa gaagaaaaga
    11641 tcatcatcag tagtattgat agggtatccc agattaatag gggctgtcca gcggccatca
    11701 gcttccagct gcgaaacaaa gatatcgtag ccccccatgt tactatggcc ctcggagctg
    11761 aagtacaggg ataatccatc ggaggtgagg aatggagtgt tttcgttaag tttggtattg
    11821 attgttgacc caaggttttg ggggacttcc caattatctc cagattcccg gtggctgacg
    11881 taaatatcca gatcgccgaa tccacccttt cggttgctgc tgaggtatag gtccttgcca
    11941 tcggggctaa gacaggcatg tgtttcgtaa tattcggagt tgatgtttgg gttgagcttt
    12001 ctaattggtg tccatctgcc attttccaga tgggaaacat aaatgtttcc atcatgatta
    12061 tcgtccctga agaggtagag ctcatcgcct ctgtacgata gcgaaagggt ttcgcatcga
    12121 ccgtcaacct gca
//
LOCUS       DAOPHX010000078        11939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_6397_length_11939_cov_4.822787, whole genome shotgun sequence.
ACCESSION   DAOPHX010000078 DAOPHX010000000
VERSION     DAOPHX010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 11939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11939
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6397_length_11939_cov_4.822787"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..717)
                     /locus_tag="PK502_06950"
     CDS             complement(178..717)
                     /locus_tag="PK502_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00590.1"
                     /translation="MSSTSEREALVKAFEKRLSEASDEAIIGMLEEIRESGESYMIPS
                     LVNLLFATRGEALKQAVMNFMVDVKIQAAAEYFVSAIQNNPSSPDLYRLVSVCWQSRL
                     DFSSYIDLFLTLLCNADLQTSIEAFSVIENSLDAKSDEEVKELTSRARSMVEHAAEPN
                     KPLVAQLVKLLENFQRDEW"
     gene            complement(1053..1877)
                     /locus_tag="PK502_06955"
     CDS             complement(1053..1877)
                     /locus_tag="PK502_06955"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPH00591.1"
                     /translation="MSIRLVATDLDGTLMRSNGEFSVEDIGTLCRLGKLGVVRVIATG
                     RSPYSASRVLSPDFPIDYLVFSSGAGILKWDTKTLVHTNEMPAALVQTIISWLCFHGI
                     DFMVHNPIPLNHYFQYFTAGNSNPDFERRLNLYKEFATPLIQGVPYPNPASQVVAILP
                     NDEGMFNSLRSGVEGVKIIRATSPIDGSSIWMEIFPETVSKASGIRWICQNLEGVTPD
                     QIVVVGNDYNDSDMLQLTAHSFVVENAPELLKKSYTVVPSNDSSGFSYAINKVLGM"
     gene            2024..2284
                     /locus_tag="PK502_06960"
     CDS             2024..2284
                     /locus_tag="PK502_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPH00592.1"
                     /translation="MTKIITFNELRRVKDNLPAGSMQVIADRLNINVETVRNYFGGTN
                     YKDGAAVGFHVEQGPDGGLVTLDDTTILDIAYEILGNPRELV"
     gene            2368..3219
                     /locus_tag="PK502_06965"
     CDS             2368..3219
                     /locus_tag="PK502_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPH00593.1"
                     /translation="MDFDFNVQKIEEAYRHELLSYLNQLFTGVNLPSHDISHHERVWR
                     YCRSLLLEINRFGLDVPADLVENAIVACYFHDTGLTINLGESHGALGAEICSRYLQQK
                     PNFTSFRNKEILTAIEFHDDKSIRTEENGDALSMLNLTRLVSTADDLDAFGTIGVFRY
                     IEIYLKRAVAANELPGRVLTNLQNRYSNFKSAYALLEKFVDRQECRYYQTFNFFTRLA
                     TEVTLGVGSANGPYGVYRVIKNNLVEKGQSIEDVIDYVNENPISEYAQSFFNVLKVEL
                     NINSTVS"
     gene            3223..4503
                     /locus_tag="PK502_06970"
     CDS             3223..4503
                     /locus_tag="PK502_06970"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HPH00594.1"
                     /translation="MQPVQKFDVVVIGGGPAGLLAGGRAGQLGMKVAILEKMEKPARK
                     LRITGKGRCNITNLKSYDDFVGEIVPNSHFLAKAFGNFFSSDIINLLESNGVPTVTER
                     GQRVFPASGKAWDVAEALVGWAKRCGAQLFNHYEVESLLVSGSAITGLKVRVNKLSES
                     IVFSCKAIVVATGGKSYPATGSTGDGYRFAQQVGHTLTDLRPALTGIETHPAFSFTGR
                     LTLKNVQTTLWVDGAVAGEEFGEVELADYGLSGPAILKLSLKSVDAFRSNRKVRITID
                     LKPALTEEKLEARIIRDTSRSGYFSLEMLLRGLLPPPLVPVYLRLMNLKPKLQAALLN
                     EAERKKLVGHLKNLSFEMTGYRGWNEAIVTAGGVSLNEVDDSTMASKLVQGLFLAGEV
                     LDLTGNTGGYNLQIAFSTGWLAGQSAAAFVQKNA"
     gene            complement(4573..5184)
                     /locus_tag="PK502_06975"
     CDS             complement(4573..5184)
                     /locus_tag="PK502_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPH00595.1"
                     /translation="MKIICIGRNYMEHAHEMNSEVPEKPVFFMKPDTSLLRNNQPFFY
                     PDFTQELSYEVEVVLRVNRLGRNINRRFAHRYYDAATLGIDFTARDLQRECKQKGLPW
                     EVAKAFDYSAPVGDFIPKKELADINHLPFRLEINDRLVQAGNTSEMIFSFDELIEHVS
                     KFVTFRTGDLIFTGTPSGVGQVKIGDRLKAYIEDRLILDFFVR"
     gene            complement(5571..7211)
                     /gene="pruA"
                     /locus_tag="PK502_06980"
     CDS             complement(5571..7211)
                     /gene="pruA"
                     /locus_tag="PK502_06980"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HPH00596.1"
                     /translation="MNNAIFKYDFPKNEPILGYLPGSPERLALEAEIKKLSSEVMDIP
                     LIIGGKEVRTGKTGKVVMPHDHQHVLANYHIAGEEEVKMAIEAALKAHEYWGTVSWVE
                     RLSITVKAAELLSTKYRALINAATMLGQDKNVYQAEIDSACEAIDFLRFNAYYISEVY
                     NHQPSSDKSSINRLEYRPLEGFVFAVTPFNFTAIASNLNMSPVLMGNTVVWKPATTAI
                     LSNYILMKVYKEAGLPDGVINFIPGKGSAIGNIVLNHPMLAGIHFTGSTATFNQFWKT
                     VSNNIGHYRSYPKLIGETGGKDFMFLHSSANINEAVTAAVRGAFEFQGQKCSAASRAY
                     IPQSLWPEFKSKMTATLKEIKQGDPTDYETFINAVIDEASFDNIMQYINQAKTSDKAE
                     IIWGGKGDKSRGYFVEPTVILTTDPHFVTMEEEIFGPVLTIYVYPDNKFNETLEICNS
                     TSPYALTGAIFATDRMALNHACRVLRYAAGNLYFNDKPTGAVVGQQPFGGARASGTND
                     KAGGPLNLIRWTSPRTIKENLIPPVNYKYPYMTNGDSK"
     gene            7602..9149
                     /locus_tag="PK502_06985"
     CDS             7602..9149
                     /locus_tag="PK502_06985"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPH00597.1"
                     /translation="MHEKDSKVEELKKRKEQILQGGGEKAVAKQIAMGKLTARERIIA
                     LLDEDSFHEYDMFVEHEARDFGMEGKVLYGDGVIIGTGTIYGAPVAVFAQDFTVAGGS
                     LGSMHARKITKIMDYALRMRIPLIGINDSGGARIQEGVNSLAGYGEIFWRNTLNSGII
                     PQISVILGPCAGGAVYSPALTDFVFVVDNISKMFITGPDVIKSVLGEEISQEDLGGAK
                     VHAQITGNAHFYAESEYECFEQIKKLITFIPWNNTRKARRFEPKDPKACYNIEEIVPG
                     DPKQPYDVRDVIRAIVDDSNFFEIQEMWAGNIVIGFGRMNGETVGFVANQPLVMAGVL
                     DVDSSDKAARFIRYCDAFSIPLCTLVDLPGYLPGIDQEHAGVIRHGAKLLYAYSEATV
                     PKLTVILRKAYGGGYIAMSSRHLRADFVFAWPGAEIAVMGPEGAANIIFRKEISEAAN
                     PEEMRRQKVEEYKEKFANPYVAAAKGYIDTVIEPAETRKLLLHALEVASTKVSDRPFK
                     KHGIPPF"
     gene            9161..9520
                     /locus_tag="PK502_06990"
     CDS             9161..9520
                     /locus_tag="PK502_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HPH00598.1"
                     /translation="MTKIDINGNTQDTPKLNDSQLNTLAVEDGEYKTLFTPKFSGYKK
                     WEQHNEKLIRAFIPGTIVEVFVKPGQPVKQGDMLLILDAMKMNNQVKSPINATVKEVN
                     VKVGDKVSKGVDLVVLE"
     gene            complement(9574..10395)
                     /gene="ispE"
                     /locus_tag="PK502_06995"
     CDS             complement(9574..10395)
                     /gene="ispE"
                     /locus_tag="PK502_06995"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPH00599.1"
                     /translation="MIVFPNAKINIGLNIVDRRSDGFHNISTFFYPVKGLCDMLEVII
                     AEDQDLPLTFSQTGVETNCPANENLCVRAVGLMSKRLALPPIAMHLHKVIPTGAGLGG
                     GSADGSFTLVAINELLGNPLGKKELMELSLQLGSDCPFFIENQPCLAEGRGEKIRPLK
                     IDLSGYFLLVVKTDIQVETGKAYSETKPAPWGVSLVNLIKDDINTWRLTIKNDFEEII
                     FTRYPKIGKIKKRLYGMGAIYAGLSGSGSAVYGIFDREPKDWVKREGYFIRCVRL"
     gene            10813..11475
                     /locus_tag="PK502_07000"
     CDS             10813..11475
                     /locus_tag="PK502_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPH00600.1"
                     /translation="MTKRLVHVMAFLMLTSMGVNAQKFSFGVFVDPQLTWFNSDTKRY
                     DPNGPVAGFNIGFSGEKYFAQRYALSSGLSLNTLGGNIRFMQDTFTLKTNDGEYTVTP
                     QTNVKFRAQYLTLPVSLKFRSTQIGYTSFYANVGFKGSIRLKGFAWYSPQNVDREVAN
                     DHFAWGFVSYFIGLGMEYSLGGESALQCGLSFSDGLTGMLDTSNGKITSQSLTLRLGI
                     IF"
     gene            11514..>11939
                     /locus_tag="PK502_07005"
     CDS             11514..>11939
                     /locus_tag="PK502_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPH00601.1"
                     /translation="MRIALAQLNYTINHFDLNRGKIVDAIERAKSEGADLVVFSELAV
                     CGYPPRDLLEQEEFIQKCYSSINAIASCCSGITAIVGAPSLNPNPKGKMLYNSAFVLQ
                     NGVVHSIHGKTLLPDYDIFDEYRYFEPADDGYDLVEVAGK"
BASE COUNT         3178 a         2570 c         2791 g         3400 t
ORIGIN      
        1 atattaattg aaggatatta gaaaagtacc gttcccaaca gcgtgtttat tttatttggg
       61 ctttaattag tttgcaaaat ttatcgcttt tgggtcactt tttatcgaat agatagagca
      121 ttgttccaat cccccaaaca aaacaaatac gcgaccgata tagtcaacct ataattgcta
      181 ccactcatca cgctgaaaat tttccaacag cttaaccagc tgggcaacca atggtttatt
      241 gggttctgct gcgtgttcaa ccattgacct tgctctgctg gttagctcct ttacctcttc
      301 atccgacttg gcatcaagtg agttttcgat tacagagaag gcttcgatgc tggtctgcag
      361 atcagcgtta cacagtaagg taagaaatag gtcaatataa gagctgaaat caagccggct
      421 ttgccagcac accgagacca gcctgtacag gtcgggtgaa gaggggttat tttgaatggc
      481 gctcacaaag tactcggcag cagcctgaat tttgacatca accatgaaat tcattacagc
      541 ctgtttaagc gcttcacccc tggtggcaaa aagcaggttg accagagagg gaatcatgta
      601 gctctcacca gattctctta tttcttcgag catgccaatt atagcctcgt ccgaagcctc
      661 agatagcctt ttttcaaaag cctttactag tgcttctctc tcactggtgc tactcatagc
      721 tgttctttta attgcaaaat taacaatgtt ttaccttttt tatccttaga ttttcatgac
      781 ttttttaaaa ccccgaagga gcgaaacata ctaaccctag gcaacgcccg gggtttaata
      841 aaaaccgaaa atatctgcga tgcaagcagt atctccccaa gagccgttta gttcctttgc
      901 atcgtaatag gaaaaaattt ccttcgaggc atgagtagat cagaggtatt tttcttcctt
      961 ttacccttca actaattcag ggcatgcttt tagcttcttc ccggtttacg tcgtgcctct
     1021 cgatgttttt gtgatattga aatggttcgt agttacatgc ccaacacctt gttaatggca
     1081 tagctaaacc ctgaactatc gtttgatgga acaaccgtat aggatttttt tagcagttca
     1141 ggggcgtttt ccacaacaaa gctgtgcgct gtaagctgga gcatgtcgga atcgttgtag
     1201 tcatttccca ccacaacaat ttgatcggga gtaacacctt cgaggttctg acaaatccac
     1261 ctaattccgc tagccttgga aacggtttca gggaaaatct ccatccagat tgaggagcca
     1321 tcaattggtg aagtagcccg tataatcttc accccttcaa ctcctgaacg caagctgttg
     1381 aacatgcctt cgtcattggg tagtatggct acaacctggg atgccggatt ggggtacgga
     1441 actccctgaa ttagaggcgt ggcgaattcc ttgtaaaggt tcaagcgtct ttcaaaatcg
     1501 gggttgctgt tgcccgcggt gaagtactgg aagtagtggt tgagcggaat ggggttatgc
     1561 accataaaat caatgccatg aaaacaaagc catgaaataa tggtctgaac tagagccgcc
     1621 ggcatttcat tggtatgcac aagggtcttt gtgtcccatt ttaaaattcc agctcctgat
     1681 gagaaaacca ggtagtctat tgggaaatca ggcgaaagaa ccctggatgc ggagtatggc
     1741 gaacggccgg tggcaattac ccttacaaca cccagcttac ctaatcggca tagtgtgcca
     1801 atatcttcaa cgctaaattc accattgctc cgcatgaggg tcccgtccaa gtcggtagcc
     1861 actaatctta tactcatgag ttttattttt tcccaaatat aggacgatta gcggtttttt
     1921 gcagtctggc taaattcaaa ataaaatgtg ttaaataatt tttattttag gattaattgc
     1981 atatatttaa aacgttaaac tcattatcta aaccgctaga attatgacaa aaataatcac
     2041 atttaatgag ctcaggaggg ttaaggataa ccttccagcc ggcagcatgc aggttattgc
     2101 ggacaggttg aacattaatg ttgaaactgt ccgcaattat tttggcggca ccaactacaa
     2161 ggatggagca gcagttggat ttcacgttga gcagggcccc gatggaggac tcgttactct
     2221 tgatgatacc acaatactcg acatagctta cgaaattctt ggtaacccga gggagttggt
     2281 gtaaacaaca ttctttttgc ttcgttgtaa aaacaggata cctttggcga tatcctgttt
     2341 ttttatacat ctggaattct tacccaaatg gattttgatt ttaacgttca gaaaattgag
     2401 gaggcttacc gccacgagct gttatcctat cttaaccagc tatttacggg ggtaaattta
     2461 ccatcgcatg acatatcgca ccatgagagg gtttggcgct actgcaggag tttgcttctg
     2521 gaaataaaca ggttcggtct tgatgtccct gctgatctgg tcgaaaatgc aatagttgcc
     2581 tgctattttc atgatacggg tttaaccatc aatttggggg agagccacgg tgcacttggc
     2641 gctgagattt gtagcagata tttacagcaa aaacccaatt ttacctcatt tagaaataaa
     2701 gaaatcctaa cggcaatcga gtttcacgac gataagtcaa ttaggacaga ggaaaatggc
     2761 gacgcccttt caatgctcaa tcttacgcgc ctagtatcca ctgccgatga tctggatgct
     2821 tttgggacta ttggcgtttt caggtatatt gaaatctacc ttaaaagagc cgtggccgct
     2881 aatgagctgc ccggcagggt gttaacgaac ttgcaaaatc ggtattccaa ctttaaaagc
     2941 gcctacgctt tgctcgaaaa attcgttgat aggcaggagt gcaggtacta tcaaaccttt
     3001 aactttttta cccgtttagc taccgaggta accctaggtg ttggtagcgc taatggcccg
     3061 tatggagtat atagggttat caaaaacaac cttgttgaaa agggccaaag tattgaggat
     3121 gttattgatt atgtaaacga aaatcctatt tcggagtatg ctcaaagctt tttcaacgtg
     3181 ctcaaggttg agctcaatat aaattcaacc gtaagttaga caatgcaacc tgttcaaaaa
     3241 tttgatgtag tagtaattgg tggaggccct gccggtctcc tggcgggggg cagggccggc
     3301 caactcggca tgaaggtggc tattctcgag aaaatggaaa agcccgcccg taagctgaga
     3361 ataaccggaa aggggcgatg caatataacc aaccttaaaa gctatgacga ttttgttggg
     3421 gaaattgtcc ccaactcgca ctttctggca aaggcttttg gaaatttttt cagtagcgat
     3481 atcattaacc tgctggaatc gaacggtgtg ccaaccgtta ccgagcgcgg ccaacgtgtt
     3541 tttcctgcct cgggaaaagc atgggatgtt gcagaagccc tggttggctg ggccaaaagg
     3601 tgtggagcac agctgtttaa ccactatgag gttgaaagcc ttttagtaag cggtagcgca
     3661 ataaccgggc ttaaggtaag ggttaataag ctctctgagt ctatcgtatt ctcatgtaaa
     3721 gcaatagttg tagctaccgg aggtaaatca tatccggcaa cgggatcaac tggcgatggc
     3781 tacaggtttg cccaacaggt tggacatacc ctaaccgact tgaggccggc gcttaccggc
     3841 attgaaaccc atcctgcttt ttcgtttaca ggaagactta ctctgaagaa tgtgcaaaca
     3901 actctttggg ttgacggggc cgtggctggt gaagaatttg gtgaagttga acttgccgat
     3961 tacggcttaa gtggccctgc cattttaaaa ctaagtctga aatcagttga tgcattccgg
     4021 tcgaatcgaa aggtaaggat taccattgat ctaaaacctg ccctgaccga ggagaagctg
     4081 gaggcaagaa tcattaggga tacctccagg tcgggatact tcagccttga aatgctgctt
     4141 aggggtttgc tacctccacc cctggtgcct gtttatttaa gattgatgaa ccttaagccg
     4201 aaactacaag cagcattact aaacgaagcc gagaggaaaa agctggttgg gcatcttaaa
     4261 aatttgtcat ttgaaatgac cgggtacaga ggttggaacg aagctatcgt tactgcaggt
     4321 ggagtttcac taaatgaggt tgacgatagc acaatggcct cgaaactagt ccaaggcctt
     4381 tttcttgcgg gtgaggttct tgacttaacc ggaaacaccg gcggttacaa cctccagata
     4441 gccttttcaa cgggctggtt ggctgggcaa tcggccgctg cttttgtcca aaaaaatgcc
     4501 tgaatgaagt ttatcgcagg actacagctt agcatgtgat taaagcaacg taacccctcc
     4561 tttggacaat gcctacctga caaagaagtc gagtatcagc ctgtcctcga tataggcttt
     4621 tagcctgtca ccaatcttta cttgccctac tccagaagga gtgccggtga aaattaaatc
     4681 gccagttcgg aaggttacaa atttcgaaac atgctctata agctcatcaa aagagaaaat
     4741 catttctgat gtgtttcctg cctgcactag gcggtcgttt atctcaagtc tgaacggtag
     4801 atggttaata tcggcaagtt cctttttggg tatgaaatca ccaaccgggg cggagtagtc
     4861 aaaagctttg gcaacctccc acggaagccc tttttgttta cactcgcgct gaaggtcgcg
     4921 agccgtgaaa tcgattccaa gggtggctgc atcgtagtac ctatgtgcaa aacgcctgtt
     4981 gatgttacgg cccagccggt ttaccctaag caccacttca acctcgtagc ttagctcctg
     5041 ggtaaaatca ggataaaaaa atggctggtt atttcgcaga agactggtgt cgggtttcat
     5101 gaaaaatacc ggtttctcag gaacctcgga gttcatctca tgggcatgct ccatatagtt
     5161 ccgaccaatg caaataattt tcatcagtat taagtgtgta tgtagttatt aatcagatcg
     5221 ataagtttat gcccctcttt tattccttaa taatgaagag ctatcttaca ctactgactt
     5281 catgctaaat ttttcatgct ctgctggctt tcctcttagt ggaagtagag taaaagccat
     5341 attgattaat tcctccttat cgtcaagatt gcacctcgaa aaatgcaaca atttttactt
     5401 acttcaaagg gtagccaaaa attgtatttg attgaagcct aagctggagt tgagggattt
     5461 tatttagtgg gcaaatttcg gagtaaaaag ctacacttac cctttactta aaaaatgaaa
     5521 gagccacaat gttaccgtgg ctctttccct cttacagcta tgaatgaaaa ctacttacta
     5581 tccccgttag tcatataggg atatttgtag ttaaccggag gtattaggtt ttccttaatg
     5641 gtacgtgggc tagtccaccg aatcaggttg agaggacctc ctgccttgtc gttagttccg
     5701 ctggctcttg ctccgccaaa tggttgttgg cctaccacag cacccgttgg cttatcgttg
     5761 aaatataggt ttcctgcggc ataccttagc accctgcagg catggttgag cgccattctg
     5821 tcggtagcaa aaattgctcc ggttaatgcg tagggcgatg tgctgttgca gatttcaaga
     5881 gtctcgttga atttattatc ggggtaaacg tagatggtga gcacggggcc aaagatttcc
     5941 tcctccatgg ttacaaaatg cgggtcggtt gttaggatta ccgtaggctc aacaaaatag
     6001 cctctcgatt tgtcgccttt tccaccccag atgatttcgg ccttgtcaga agttttagcc
     6061 tggttgatgt actgcatgat gttgtcaaac gaagcttcat caatcaccgc atttataaac
     6121 gtctcataat cggtggggtc gccctgctta atctctttca aagtggctgt catcttgctc
     6181 ttaaattccg gccatagcga ttggggtatg taggctctgg aagcggcaga gcatttttgt
     6241 ccctgaaatt caaacgctcc tcttacagca gcagtaaccg cttcgttgat gtttgcgctt
     6301 gaatggagga acataaagtc ttttccgccg gtttctccaa taagcttagg gtaggagcgg
     6361 tagtggccta tgttgttgct tacggttttc caaaactggt taaaggtagc agttgacccg
     6421 gtgaaatgaa ttccggccag catggggtgg ttaagcacta tattgccaat tgctgagccc
     6481 ttaccgggaa taaagttgat gacaccatcc ggaagtccgg cttctttgta aaccttcatc
     6541 aggatgtagt tggagagtat ggctgttgtg gcgggtttcc atacaacggt gttgcccatc
     6601 agcacaggcg acatgttcag attggaggct attgcggtga agttgaacgg ggttacggca
     6661 aatacgaagc cctccaatgg tctgtattcc aacctgttga tgctactttt atccgatgag
     6721 ggctggtggt tgtaaacctc ggaaatgtag taggcattaa acctcagaaa atcaatagct
     6781 tcacatgctg agtcaatttc tgcctggtac acgtttttat cctgcccaag cattgttgca
     6841 gcgttgatga gcgctctgta cttggttgaa agcagctcgg ctgcttttac ggttatgctc
     6901 aacctctcaa cccaagaaac cgttccccag tactcgtgag cttttagagc agcctcaatg
     6961 gccatcttaa cctcttcctc gccggcaata tggtagttgg ccagtacatg ctgatggtcg
     7021 tgtggcatta ccacctttcc ggtttttccc gtgcgaacct ccttgccccc aattatcagg
     7081 ggtatatcca ttacctccga tgacagcttt tttatttctg cttcaagtgc cagcctctct
     7141 ggcgaaccag gtaggtaacc caggatgggt tcatttttcg gaaaatcgta cttaaaaata
     7201 gcgttgttca tagctttagt tagttatttt attcgttatg caaatgtatt catatcgaac
     7261 taaaaaacaa atgataatta tcaggaataa gttgaaaagt aggttaagac caatttttct
     7321 gaggcagttg tttttactta aaactcgcca aaaacttaac aagaatatat cccttatgtt
     7381 cacccgtact attgcatatt tcggttacgc taatgtgctt gggcggtaac tgaatagtca
     7441 agtcatcaat gtaacatcaa taaaatcagc atgttaatac tcttacaggc taaaaaagaa
     7501 gtcaatattc ttttatatat ggccgctatt tagtaacctt gcactttcga tgtatgatga
     7561 ggattacagg tggacaattt actaaccaaa caaatcaagc tatgcatgaa aaagatagca
     7621 aagtagagga gctaaaaaaa cgtaaagagc aaatcctgca gggtggcggc gaaaaggctg
     7681 tagctaagca aatagctatg gggaagctta ctgcccggga gcgaatcatt gccctgcttg
     7741 acgaggactc gtttcacgag tatgacatgt ttgtagagca cgaagcccgc gattttggta
     7801 tggagggtaa ggttctgtat ggcgatgggg ttataattgg tacgggaacc atttatggag
     7861 caccggtggc tgtttttgcg caggatttta ccgttgccgg aggttcgtta ggctcaatgc
     7921 atgcccgtaa aatcaccaag ataatggact atgcgctccg aatgaggatt ccccttattg
     7981 gaatcaacga ttccggaggt gctcgcatac aggaaggggt taattcactg gccggatacg
     8041 gggaaatttt ttggaggaat actcttaaca gcggaattat tccccagata tcggtcatac
     8101 tcggcccatg tgccggcggt gcagtttact caccggcgct aaccgacttt gtgtttgtgg
     8161 tggataatat ttccaagatg tttatcaccg gccctgatgt cattaagtcg gtgctgggtg
     8221 aagaaatatc gcaggaggat ttgggcggcg caaaggttca tgcccagatt acgggtaatg
     8281 cccattttta tgccgaaagc gagtatgaat gcttcgagca gattaaaaag cttatcacct
     8341 tcattccatg gaacaacacc cgcaaagctc ggcgctttga gcctaaggat cctaaagcgt
     8401 gctacaatat tgaggagatt gtccctggcg atccaaagca accctacgat gtgcgcgacg
     8461 tgattcgggc tattgttgac gattccaact tcttcgagat acaggagatg tgggcgggta
     8521 acattgtaat tggttttggg cgaatgaatg gcgaaacagt gggctttgtt gccaaccagc
     8581 cgctggttat ggccggtgtg cttgatgtgg acagctccga taaggctgcc cggtttattc
     8641 gttactgcga tgcttttagc ataccgcttt gcaccctggt tgaccttcct ggttacctcc
     8701 ccgggattga ccaggagcat gcaggggtta ttaggcatgg cgccaagttg ctttacgcct
     8761 acagcgaggc tacagttccc aagctgacgg ttattttacg taaagcgtac ggcggaggat
     8821 acattgccat gagttcacgc cacctgcgtg cagattttgt atttgcatgg ccgggtgctg
     8881 aaattgccgt tatgggcccc gaaggcgcag ccaacatcat tttccgcaag gagatttcgg
     8941 aagccgccaa ccccgaagaa atgcgacgcc agaaggttga ggagtacaag gaaaaatttg
     9001 ccaaccccta cgtggctgct gctaagggtt acattgatac ggtgattgaa cccgctgaaa
     9061 cccgcaagct tttgctccat gctcttgagg tggccagcac caaggtttcc gatagaccat
     9121 ttaaaaaaca cggaattcct ccattttaaa aagttggaat atgacgaaaa ttgatattaa
     9181 tggtaatact caagatacgc ctaaattgaa cgattcgcag ctcaatactt tggcggttga
     9241 ggatggtgag tacaaaacct tattcacccc gaagttttcg ggctacaaga agtgggagca
     9301 gcataatgag aagcttatcc gggcttttat accgggtact attgttgagg ttttcgtaaa
     9361 acccgggcag cctgttaagc agggtgatat gctgcttatt ctggatgcca tgaaaatgaa
     9421 caaccaggtt aaatcgccca tcaacgccac cgttaaagag gtgaatgtta aggttggcga
     9481 taaggtaagc aagggtgttg atctagttgt tcttgagtag ctacacttgt gaagcatgct
     9541 atatgaaaag ggcgccacag cgcccttttt tatttatagc cttacacaac ggatgaagta
     9601 gccttctctt tttacccaat cttttggctc tctgtcaaaa atgccgtaca ctgccgatcc
     9661 acttcccgat aaccccgcat aaattgcacc cataccgtac agcctttttt ttattttccc
     9721 tattttgggg tagcgggtga aaataatttc ctcaaaatcg tttttaatgg tcaaccgcca
     9781 ggtgtttata tcgtccttta taaggtttac gaggctcacg ccccatgggg caggtttagt
     9841 ttcggagtac gcttttccgg tttcaacttg aatgtcggtc ttcacaacca gcaggaagta
     9901 tccggagagg tcaattttca agggtctaat tttttcgcca cgcccttcgg caaggcaggg
     9961 ttgattttcg ataaagaaag ggcaatcgct gccaagctgc agggatagct ccatcaattc
    10021 ctttttccct aaaggattgc caagcagctc gttaatggca acaagcgtaa aggagccatc
    10081 ggccgaacca ccccccagac cggctccggt ggggataacc ttgtgcaggt gcatggcaat
    10141 tgggggtaaa gccagtcgtt tactcattag tccaaccgcc cttacgcaaa ggttctcgtt
    10201 ggcagggcag tttgtttcaa ctcctgtttg gctgaaggtt aagggcaaat cctgatcttc
    10261 ggcaattatg acctccagca tatcgcataa accctttacc ggatagaaaa aggtagaaat
    10321 attatggaaa ccatcgctac gtcggtcaac aatgttgagt ccgatgttga ttttagcatt
    10381 ggggaagact atcattatcc atttttacgg caaatttagc aaatgttcat ttcgacacac
    10441 atcgtaaatt tgcaaccttg gctaccctta acctttggtt aaacttgaaa tggtttgaat
    10501 gatattgttg taggaatgca gaagtaagca tgttaatgcg caatgcagct gaaaatggtt
    10561 ccttttcttc tatatcacta gaacagaaaa gtggaacatg gattacactg atactactga
    10621 caaacacggg taaaaaatct gtgcaaatat gtctcttctg ctttgttgga gtgtcagtaa
    10681 aagtttttcc tgcgtgtgag ggttaattga aaattatcat gtacgtttgt atcccattct
    10741 tttgtacccg taagagttac ttcaaaatga tttatgctag actactattc taataattaa
    10801 ttttttagga tgatgacgaa gaggttagtg catgtgatgg catttttaat gcttacaagc
    10861 atgggggtta atgcccaaaa gttcagcttt ggtgtttttg ttgacccaca gcttacttgg
    10921 ttcaactccg ataccaagcg ctacgatccc aatgggccag tagcagggtt caacattggc
    10981 ttttcgggcg agaagtactt tgcccagcgg tatgccctat catctggact atcgttgaat
    11041 actctgggtg gaaacatcag gtttatgcag gataccttca ccctgaaaac caatgatggc
    11101 gaatacaccg ttactcctca aacaaatgtt aagtttaggg ctcagtacct taccctgccg
    11161 gtgagcttaa agtttcgatc tacccaaatt ggctacacca gcttttatgc aaatgttggg
    11221 tttaagggca gcatcaggct aaaggggttt gcatggtaca gcccacagaa tgttgacagg
    11281 gaggtggcaa acgaccattt tgcttggggt tttgtttcct actttattgg gctgggtatg
    11341 gagtactctc taggtggcga aagcgctctt cagtgtggcc tatcgttttc cgacggtcta
    11401 accggaatgc ttgatacatc taatggtaaa attacttccc agtcgcttac tttgcgcctt
    11461 ggaataattt tttaaacggt tttaggcttg tcgggccaaa tgaattttgc aaaatgagaa
    11521 ttgcgcttgc ccagctcaac tacaccatca accattttga cctgaacagg gggaaaattg
    11581 ttgacgctat cgaaagggct aaatcagaag gagcagacct ggtggttttt tccgaactag
    11641 ctgtatgtgg ctaccctccg cgcgacttgc tggaacagga ggagtttatt cagaagtgct
    11701 acagctctat taacgccatt gcatcctgct gtagcggtat aactgccatt gttggcgctc
    11761 ctagccttaa cccaaatcct aaggggaaga tgctctacaa ctctgctttt gtgttgcaga
    11821 atggcgtagt tcacagcata catggcaaaa cgcttttacc cgattacgac atatttgacg
    11881 agtacaggta ctttgagcct gcagacgatg gctacgatct ggttgaggtt gccggaaaa
//
LOCUS       DAOPHX010000079        11921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_6408_length_11921_cov_4.438733, whole genome shotgun sequence.
ACCESSION   DAOPHX010000079 DAOPHX010000000
VERSION     DAOPHX010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 11921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11921
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6408_length_11921_cov_4.438733"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /locus_tag="PK502_07010"
     CDS             <1..1376
                     /locus_tag="PK502_07010"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPH00602.1"
                     /translation="YRVDRESQFNIYKDYSYSSSVIYKTRLKSGNRYITTVYAFVSPD
                     FDGENVRLSAGGNYMGYRACYYDMERKGQWQKLTINSYGEEGAEYYANLYFEYRKSTD
                     FSQLKGHVVFAYPTYSVIKDNPQNPASYTASDFAREYPLTGENSTIVPDGTAGCRFDA
                     TTQGRTWRSQFHSTTDFQQARVVKGDSVFASVYCFVSPDFNGNDVRLELRGPVKGQTL
                     SRFNPNERGRWVKLEAKAIADKEGIVSGIFLFMQNNVKDFSALKGYVTFAYPQLYVKP
                     KVGLTEVISDYKINLAGFAGLGLQLGTSVDSTGSDGEFKFSMADDRFAGPRVDRWRYA
                     IYLYRHEYTPLQKIFGGGFGYTRKFATQFFPDDQQRDYDYPHNPFLSILLYSGMLGLL
                     ISVWFIYQVFRLYWSHRKVLWPFGLAFLASFFYAFFSSNNFFEPAIFGVLAFIPFYYK
                     YLSVVKD"
     gene            1491..3554
                     /locus_tag="PK502_07015"
     CDS             1491..3554
                     /locus_tag="PK502_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00603.1"
                     /translation="MSKVRENLLVVVLVLFIARMGLPQYLKYILFPAVGLYGVWALWQ
                     FVEDRRWQGFRLRNTLIFTPLLVSMLIYFIMLVFTPNPQINLLRDAFNVLVFFSFVIA
                     LYLISYTPSGYQKVLNQVALYTFIISSLFAFLGVLKLVLQLYGITFEFLEVEMLGYPL
                     GTSLSVDNNSFAILCLLGLVLAIPYTTRKLKIRYSLLLQLGLTLIVVSAFFSTSRRGL
                     IIALLCLLICGLTWLVSIPFRSERLKNLRVNTSFFLLLSIMVIGNFYWFVNHMSPIER
                     YRFLYSHHFEKFEAIHFINRMAVQEQLISNGNTEYSDVEWKLWGTEFDPRYPYTGWAE
                     NNFKLVGEIKGKGAELVPEGAEAALVDSSVQGSTWGGHAYYYSILFEAKGEAGSWYMA
                     SVYCYVSPDFSGDNVEIGVEHAISSTTEKAIYDLQASGSWQKLEVTFQADTAAYKVGL
                     LMRVNNALNIKGLKGCMEFAYPELYKIQTHREELLASKVENKVDQQQQNMQNVNPMEE
                     EVVENPEDRQSDWFSSDARVLHNFLSLADSSGRFRHSMSNNFFSGPRIDRWRYALYIY
                     WHEYSLVQKIFGGGFGYTRKFTDMFRDQWRVTEYDYPHSPFLSVMLYSGIFGLIFYIW
                     LLLGAVKYYWTYRRDYWPFGLAFVVAFFFAFFSSNNPFEPAVLAVFTTIPYFAHYFYL
                     VEKHG"
     gene            3547..4602
                     /gene="gmd"
                     /locus_tag="PK502_07020"
     CDS             3547..4602
                     /gene="gmd"
                     /locus_tag="PK502_07020"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005469633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPH00604.1"
                     /translation="MADKLKKALITGITGQDGSYLAELLLGKGYEVHGVIRRASTFNT
                     QRIDHLYSDRHNKDVSMFLHYGDMTDTSSLNRLLEKVAPDEIYNLAAQSHVKVSFEVP
                     EYTAEVDGIGTLRFLDAIKDVGLRTRFYQASTSELYGLVREVPQNETTPFYPRSPYGA
                     AKLYAYWITVNYREAYGIFASNGILFNHESPRRGETFVTRKITLAATRIKAGLQDKLY
                     LGNLDARRDWGYAPEFVEGMWRILQHDTPGDFVLATGETHSVREFVELAFKLIGMDVE
                     WKGVGINETGVDKNTGKVVVAIDPDYYRPTEVDLLVGDASKVDKLLGWKPKVQFEELV
                     EIMVKADWDRLSINGRV"
     gene            4937..6004
                     /locus_tag="PK502_07025"
     CDS             4937..6004
                     /locus_tag="PK502_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HPH00605.1"
                     /translation="MDSNSKIYIAGHRGLVGSALERKLRQKGYYNIVVRTHAELDLLC
                     QQDVFNFFASEKPEYVFMAAARVGGIMANNTYRAQFIYENIQIQNNVIHAAYLNGVKK
                     LLFLGSSCIYPKDSPQPIKEEYLLTSPLEYTNEPYAIAKIAGIKMCESYNLQYGTNFI
                     SAMPTNLYGPGDSYDLAKSHVLPALIRKMYLGKCLSENRWEDLRHDLNKWPVDGVDGE
                     SSKNEIFAVLGKYGISETSITFWGSGKPMREFLHSDDMADASVFLMENVDFDALKGHG
                     EIRNTHINIGTGKDISITNLAGMVKEIAGYHGSIRWNTSMPDGTYRKMLDVGKLHSLG
                     WHHSIELEKGIGQAYTDYANS"
     gene            6053..6931
                     /locus_tag="PK502_07030"
     CDS             6053..6931
                     /locus_tag="PK502_07030"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPH00606.1"
                     /translation="MPRILIIGKNSFIGKNYREYSKNSSIDEVDAKQYKPEELNFEGY
                     DVVLHLAAIVHQSGNIGLEEYTRVNTHFPVALAEQAKMAGVGQFIFMSTSKVYGKYNP
                     AMGSWTEDSPCSPANYYGISKLRAELELLKLNDISFQVSIIRTPMVYGRGVKANMLSL
                     IKLVDGLPLLPFKRVNNRRSLTFAGNLAAYIDRIIELKTQGVFIAMDAYSPSTEELIV
                     SIAAALGKNTRLFSPGRLALNLGKHFFRGYYERLYLDEVLDNTKTLELLSFTPPYSLE
                     EGMADTISSYLQLKEK"
     gene            6934..7932
                     /locus_tag="PK502_07035"
     CDS             6934..7932
                     /locus_tag="PK502_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPH00607.1"
                     /translation="MTIVIVILLLACIVSFTLAAFIRRMAIRLRLMNESNARSLHSGS
                     IPIGGGLAIIAVWYIGTAVFFWLGFMDKSLFYALLCGLVLAGVSLLDDIIQVKPIIRL
                     IVHFGVATASLYLLGGLRKPVTPGIDILSIPAVINPLAVVGMVWFINLYNFMDGADGF
                     ASVEAISIALVLFAFTGSMELLLLVSAVLGFLYWNWPKAKIFMGDVGSTQLGFILVVL
                     GINYHNTLDFSIFNWLMISSPFWFDATLTLFRRWRNKEKLSEAHRKHTYQRFIQAGYS
                     HKELDLALIFFNGTIFLMILVYREFEIMKIPITLFTLGILYYFTRRVDRLFPFAKD"
     gene            8059..9333
                     /locus_tag="PK502_07040"
     CDS             8059..9333
                     /locus_tag="PK502_07040"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPH00608.1"
                     /translation="MKNRFLAIFRKSEILQNASILVSGTILAQAIPILLQPFLRRYFS
                     PHDFGVYSVYSSLIGILLVISSFRYEQAIVLPKRNRDASVLVTSSILFSLVFSFLLFI
                     AIILFRKGIGEALNLDDSDGFILYLIPIGVFLLSVFQVFNFWLIRQKGFMKISVNKLV
                     RRIVEGGSQSAAALTHYPMGLVLGDVAGQVGNAVTSAYQSVRCGYSPRHFSIVRSKYL
                     LDSYSDFPRYSLIPALCSTIGFLLPVVLINRYYLPEQAGFFDLAKLILSVPMALVATS
                     ISSVVLQKVSESYRLGKSFVGELKPLIAFVFIASVVEVLLIVFAGKGIFRVFFGKVWE
                     TSGIISKWLVWPFALNFITSSFTSIFVALNRIRWQSLWQVAYFLLIISLPLFRNFTFY
                     GFIRVYASLEILANLAMVTLLTIVVIKYEKKF"
     gene            9339..10433
                     /locus_tag="PK502_07045"
     CDS             9339..10433
                     /locus_tag="PK502_07045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPH00609.1"
                     /translation="MMKRVLFIEPIVAHYRRDTYRLIFSDSRFEFALLAGDSYLDIKP
                     EKVEKTIISTYRKFTLLGEEFYYLRNSLHHVRHFNPDIVVCSGVDFHHLHTLLILLWV
                     KFHPKKKFIWWSHAGLGHQGWWGQMLRKWFYSLSDHILAYSSKGKENLRCIEIPESKV
                     TVVRNSLNQEDYGFDVVRNLTDNESVQLLFSGRISRQKGLDVLIRAVSILNKNGYSLT
                     CKLVGSGDDYEYRELARSLGIGNLVDFVGPLYGKANEEIFSSSGIFVFPAGIGLSVVH
                     ALSYGLPVITTNNLISHGPELELIRQGVNGDFFEDGNPYDLAAVVEKWIHKVQRYSIE
                     IASACHNSVAELEYTPIKVSEKILKVLSSF"
     gene            10444..11232
                     /locus_tag="PK502_07050"
     CDS             10444..11232
                     /locus_tag="PK502_07050"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPH00610.1"
                     /translation="MHPELLKIRTFGRNSLLNFLSRYSEPKPGIHILNSHFLSLDGKA
                     SPEIFEELLSGMEDRGVRFISFEEAVKRITAYNLPVDECLVAFSWDDGFEECFTRIKP
                     VLDRRSLKAAFFINPGFIDGDEAYRYHFTHEVVMVDKPPMSWEQLRILSSEGHVVGAH
                     TLDHLSLDSDNTELLHRQIAGSKIRIEDKINARVEHFAFPFGRLKLLSPLGLQLALSN
                     FKYVYSQSNYRHYFSFDGKVINRRHFECDWPLEHVIYFLKSKKF"
     gene            11229..>11921
                     /locus_tag="PK502_07055"
     CDS             11229..>11921
                     /locus_tag="PK502_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00611.1"
                     /translation="MRAKVDVLVVGDFPPRTFTGISMVNALVKGMLEAKGKIVVAIDE
                     SAWRLKGLGRIFHYFSRYVDVARPLVSCRVEILYTNIPLSRFGLLRLILLCFFARIIS
                     WKTTFYGHIHRGDIRSFFTSSHVNRFLLKLSLSFFNRIIVLSKQFADEVKAINSNQVF
                     ILPNTSLLEGYRKGRQYQYNRSFLCVSNIIRSKGIGELVEAFSSPELTDFKLAIVGNI
                     SENDFYRELVDVA"
BASE COUNT         3155 a         2367 c         2775 g         3624 t
ORIGIN      
        1 ggtatagagt tgacagggag agccagttta atatttacaa agactactcg tattcctcgt
       61 ctgttatcta taaaaccagg cttaaaagtg gtaaccgcta tattactacg gtttacgctt
      121 ttgtttcgcc tgattttgac ggagagaatg tcaggctgag cgccggggga aactacatgg
      181 gctacagggc atgctactac gatatggaga gaaagggaca atggcagaag ctcaccataa
      241 acagctacgg cgaggaggga gcggagtact atgcaaactt atactttgag tataggaaga
      301 gtactgattt ttcgcagctg aagggacatg tagttttcgc ttatcctacc tactcagtga
      361 ttaaggataa cccccaaaat cctgccagct atacagccag cgattttgca agggagtacc
      421 ctttaaccgg tgaaaattca acaatagttc cggatggcac tgccggctgc cgctttgacg
      481 ccactacgca gggaagaact tggcgcagcc aatttcactc caccacagat tttcagcagg
      541 ccagggtggt aaaaggcgat tcggtatttg cttcagttta ctgcttcgta tcgcccgatt
      601 tcaacggtaa cgatgtgcgg ctggagctac gtggcccggt taagggacaa acgcttagcc
      661 gttttaaccc caatgagcgt ggtagatggg taaagttaga ggctaaggct attgcagata
      721 aagagggaat cgtttcgggt attttccttt ttatgcagaa caatgtgaag gatttcagtg
      781 ccctaaaggg gtacgttacc tttgcctacc cacaacttta cgtaaaacct aaggttggac
      841 ttaccgaggt gatttcagac tataaaatta atttggcggg ttttgccggg ctgggtttgc
      901 agctggggac ttctgttgat tctacaggct ccgatggcga atttaaattt tcgatggcgg
      961 acgacaggtt tgccggtcca agagttgacc ggtggcggta tgccatttac ctatacaggc
     1021 atgagtacac cccattacag aaaatatttg gaggcggctt tggctacacc agaaagtttg
     1081 caacgcagtt tttccctgat gaccagcagc gtgactacga ttaccctcat aatccatttt
     1141 tatcaatact cctctactcc ggaatgctgg gtttgctgat tagcgtctgg tttatttatc
     1201 aggtatttcg cctgtactgg agccatcgaa aggtgctttg gccgtttggg ctagccttcc
     1261 tggcctcgtt cttctatgct tttttcagca gtaataattt ttttgaaccc gcaatatttg
     1321 gtgttttggc atttatccca ttttattaca aatacttatc tgttgtgaag gattaacaag
     1381 tttaaatagg tgttaggtgc gctttatgct tctaatagtc tatatataag gcgaataaca
     1441 tagaaaatta gatgaatgta gatggcggaa tagtagtaac aatttaattc atgtcgaaag
     1501 taagggaaaa tctattggtc gtggtgctgg tgctttttat agccaggatg ggattaccgc
     1561 aatatctgaa gtacatcctt ttcccagcag ttggtctgta tggcgtttgg gcgctatggc
     1621 aattcgttga ggacagaaga tggcagggtt ttaggctaag aaacaccctg atttttaccc
     1681 cacttttagt ttccatgctg atatacttta ttatgctggt gtttactcct aatccgcaaa
     1741 tcaacctttt acgggatgct ttcaacgtgt tggtattttt ttcttttgtt atagcgctat
     1801 acctaattag ctacacccca tcggggtatc aaaaggtttt gaatcaagta gcactctata
     1861 cctttatcat atcttccctt ttcgccttcc tgggagtttt gaagctggtt ttgcagctct
     1921 acggaataac atttgagttt ctagaggttg aaatgctggg ttatccttta ggaacatcgc
     1981 taagtgttga taacaactcc tttgcaatac tttgcttact gggactggtt ctcgctatac
     2041 catatactac cagaaaactc aaaattcgat atagcctatt actccagctg gggttaacat
     2101 tgattgttgt aagtgcattt ttttctacct ctcgaagagg actgattatc gcattgctct
     2161 gcttgctgat ttgcggcttg acgtggttgg tgtccatccc atttaggagt gagaggttga
     2221 aaaaccttag ggttaacaca tccttttttt tgcttctatc cataatggta attggcaact
     2281 tttactggtt tgtcaatcat atgtccccta ttgagagata caggtttcta tacagtcacc
     2341 attttgaaaa attcgaggca atacatttta ttaataggat ggccgttcag gagcagctca
     2401 tctcgaatgg taacacggag tactccgatg ttgagtggaa gctttgggga accgaattcg
     2461 accctcgtta cccctataca ggctgggcgg agaataactt taagctggtg ggtgaaatta
     2521 agggcaaagg ggcagaactg gttccagaag gtgccgaggc tgctttggtt gacagcagcg
     2581 tacagggttc aacttggggt ggacatgcct attactactc catacttttc gaagctaagg
     2641 gcgaagcagg ctcctggtat atggcatcgg tttactgtta tgtttctcca gactttagtg
     2701 gtgataatgt ggaaattgga gtggaacatg ccatatcctc aaccaccgaa aaagctattt
     2761 atgacttgca ggccagtggt agctggcaga agctggaggt aacttttcag gccgatactg
     2821 ccgcatataa ggttggcttg cttatgagag taaacaatgc gcttaatatc aaggggctga
     2881 agggatgcat ggaatttgcc tatcctgagt tatataaaat ccaaacccat cgggaagagc
     2941 tattggcctc caaagttgaa aataaagtag atcagcagca gcaaaacatg caaaatgtca
     3001 accccatgga ggaagaggtt gtcgaaaatc cagaggatag gcagagtgat tggtttagta
     3061 gcgatgcaag ggtgctacac aattttttga gcttagctga ttcgtcggga cggtttaggc
     3121 actccatgag caataacttc ttttctggtc ctcgaattga tagatggagg tatgccttgt
     3181 acatctattg gcacgagtac tcactcgttc agaaaatttt tggaggggga tttggctata
     3241 cccgaaaatt tactgatatg tttcgcgacc agtggcgggt taccgagtat gattacccac
     3301 acagcccctt tctttctgta atgctctact caggaatttt cggtcttatt ttctatattt
     3361 ggcttttact tggtgctgtt aaatactact ggacttaccg cagggattat tggccgtttg
     3421 gactggcatt tgttgtagcc ttcttttttg catttttcag ctcaaataat ccattcgaac
     3481 ctgctgtatt ggctgtattt acaactattc cctactttgc acattacttt tacttggttg
     3541 aaaaacatgg ctgacaaatt aaaaaaagca ttgataaccg gaataaccgg acaagatgga
     3601 tcgtatctgg ccgagttgct tttaggtaaa ggatatgagg ttcatggcgt tatacgccgc
     3661 gcctcaactt ttaacaccca acgaattgac catctataca gcgacaggca taacaaggat
     3721 gtcagcatgt tcctacacta cggtgatatg acggacacca gcagcctgaa taggctgctt
     3781 gaaaaggttg ctccagacga gatttacaat cttgcagcgc aaagccacgt gaaggtttcg
     3841 tttgaggtgc cggaatatac cgccgaggtg gatggaattg gcacgttgcg ttttctagat
     3901 gcgattaagg atgttggcct cagaacgcgc ttttaccagg cttccaccag cgaactatac
     3961 ggtttggtga gggaggtacc tcagaatgag actactccgt tttatccccg cagcccctat
     4021 ggtgctgcta aactttatgc ttactggatt acggtaaact acagggaggc ctacgggatt
     4081 tttgcaagca atggtatact gttcaaccac gagtcgcccc gtagggggga aacttttgtt
     4141 acccggaaaa taaccttggc cgctactcgt ataaaggcag gcttacagga taaactatac
     4201 ctagggaacc tcgatgcccg ccgcgattgg ggatacgcac cagaatttgt ggagggaatg
     4261 tggcgcatcc tgcaacacga tacgcccggt gattttgttc tggcaaccgg cgaaacccac
     4321 tcggttaggg agtttgttga actagcattc aaattgatag gaatggacgt tgaatggaag
     4381 ggggttggaa taaatgagac aggcgtggat aaaaatacag gaaaggtggt tgtagctatt
     4441 gaccccgatt actaccgccc caccgaagtg gatttgcttg ttggtgatgc ctctaaagtt
     4501 gataaattat tgggatggaa gccaaaagtt cagttcgagg agctggtgga aattatggtt
     4561 aaggccgatt gggatagact tagcataaat ggaagagttt agccagtgag tattgaattg
     4621 tgagctgtag cagttaaccg agaagatgca agttaaacat gtctaatacc tgtcatgaac
     4681 ctacagaaag gaggaatccg ccacgaggcg gacaagttta tcttatcctt tacccttgtc
     4741 ttcagcccca acggggcaca ataatacaag ggttgcaagt tccttaaata gtgaaatctt
     4801 cattaattaa tgaactgaag gggtggaata gattcgtgaa ccgtggaatg tgaatcctaa
     4861 catttttacc ctttacactt actttttttc tttttttctc ctataaccct atttcaatct
     4921 ttttagcaca aaaaaaatgg actccaactc aaaaatttac attgccggcc atagggggct
     4981 ggtcggtagc gctctcgagc gcaaacttcg gcaaaagggc tactacaata ttgtggtacg
     5041 cacccatgct gaattagacc ttctttgcca acaagatgta tttaattttt ttgcctctga
     5101 gaaacctgag tatgttttta tggcggctgc aagggttgga ggtataatgg ccaataacac
     5161 ctatagagcg cagttcattt atgagaatat ccagattcaa aataatgtaa tacacgcagc
     5221 ctatcttaac ggcgttaaaa aattgctgtt tcttggcagc tcgtgcattt atcctaagga
     5281 ttccccccag cccataaagg aggagtatct tcttacctcg ccgttggagt atacaaatga
     5341 gccctatgcc attgcaaaaa ttgccggaat taagatgtgt gagtcgtaca acctgcaata
     5401 cggaactaat ttcatatcag ccatgccaac caacctgtat ggtcccggcg atagctatga
     5461 tttggccaaa agccatgtgc ttccggcgct tattcgcaag atgtatcttg gcaaatgttt
     5521 gtccgagaac cgctgggaag acctgaggca cgatcttaac aagtggccgg ttgacggggt
     5581 tgatggagaa tcgtcaaaaa atgagatatt tgctgtgcta ggcaaatacg gtatttccga
     5641 aacctctatt accttttggg gcagcggtaa gcccatgcgc gaattccttc actctgacga
     5701 tatggccgat gcctcggtgt ttttaatgga aaacgttgat tttgatgccc tgaaaggcca
     5761 cggtgaaata cggaataccc atattaacat tggaacgggt aaggatatat ctataaccaa
     5821 cctggcaggg atggttaagg agatagctgg ttatcacgga agcattaggt ggaatacctc
     5881 tatgcctgat ggaacatacc gtaagatgct ggatgtaggt aagctgcatt cactggggtg
     5941 gcaccattct attgaactgg aaaagggaat cggacaggcc tatacggatt atgccaatag
     6001 ctaaacgaca atgggcaacc aattactcag gatatattaa tcattaccca ctatgccacg
     6061 aattctgatt attggtaaga acagttttat tggaaaaaat tatagggagt acagcaagaa
     6121 ctcctcaata gatgaggttg atgctaaaca gtacaaaccc gaagagttga atttcgaagg
     6181 gtatgatgtg gttctccacc tagcagcaat agtgcaccag tcaggaaaca ttgggttgga
     6241 ggagtacaca agggtgaata cacactttcc agttgccctg gctgagcaag ccaaaatggc
     6301 tggtgttggg cagtttattt ttatgagtac ttctaaggtt tacggcaagt acaacccagc
     6361 tatgggaagt tggacagagg attcaccctg tagtccagct aattactacg ggatcagcaa
     6421 acttcgggct gaactggaat tattgaagct gaatgatata agtttccaag tttcaataat
     6481 tcgaacaccc atggtttatg gcaggggtgt aaaggccaac atgcttagcc tgattaagct
     6541 ggtggatggg cttccgcttt taccctttaa aagggttaat aaccggcgaa gcctgacctt
     6601 tgccggtaat ttggcggcct acattgatcg gattatagaa cttaaaactc agggtgtgtt
     6661 cattgcaatg gatgcctatt cccccagcac tgaggagttg atagtatcaa ttgctgccgc
     6721 acttgggaaa aataccaggc ttttttctcc ggggagatta gcactaaacc tcggtaaaca
     6781 tttttttagg ggatattacg aacgattata tttagatgaa gtgctagaca acaccaaaac
     6841 actcgaattg ctttccttta ctccccctta ttcccttgaa gagggtatgg cagatacaat
     6901 aagttcttat ctgcaactta aagaaaaata gttatgacca tagtaattgt tattttattg
     6961 ctggcctgca ttgtttcttt tacactggct gcttttatca ggcgtatggc cattcggtta
     7021 agacttatga acgagtcgaa tgctcgaagc ttacactcag gcagtattcc tattggtggg
     7081 gggcttgcaa ttattgctgt ttggtatatt ggaacggcag tttttttctg gcttggcttt
     7141 atggataaaa gcctttttta tgccttgctt tgtgggcttg tacttgccgg cgtgagcctg
     7201 cttgatgata taattcaggt aaagcccata attagactga tagttcattt tggtgtggct
     7261 actgcctctt tatacctgct gggaggttta cgcaaaccgg taacccccgg tattgatata
     7321 cttagcattc ccgcagtaat caatccgctg gccgttgtgg gaatggtctg gtttatcaac
     7381 ctgtacaact tcatggatgg cgctgacggg tttgcctcgg ttgaggcaat ttctatcgcc
     7441 ctagttcttt tcgctttcac tggaagcatg gaacttttac ttctagtttc ggctgtgctg
     7501 ggatttttgt attggaattg gccaaaagcc aaaattttta tgggcgatgt gggaagtacc
     7561 cagctgggtt ttattctggt tgtgctgggg ataaactatc acaacacgct tgatttctca
     7621 atttttaact ggttgatgat ttcgtcacca ttctggtttg atgcaaccct tacacttttt
     7681 cgacgttggc gaaataagga aaagttgagc gaggctcatc gtaaacacac ttaccaacga
     7741 tttattcagg caggctattc acataaggag ctggatttag cccttatatt ttttaatggc
     7801 acaatctttc ttatgattct agtatatagg gaatttgaaa ttatgaaaat tccaattacc
     7861 ctattcaccc ttggcatttt atactacttt acccgcaggg ttgaccgtct tttccctttt
     7921 gcaaaggatt aataccgaaa tccccatttc tgagaaacac aaattcaaaa gtaaaaaaag
     7981 cacataccac atgtcgggtt tacatcaagc cgttaagatt gttaatttga tgaacactta
     8041 gattaaaaac gaatttcggt gaaaaaccgc tttttagcta tttttagaaa atctgaaatc
     8101 cttcagaatg cttcaatact ggtaagcggt accattttgg ctcaggccat acccatactg
     8161 ctgcaaccat ttcttcgtcg ctacttctca ccccacgatt ttggggttta ctcggtttat
     8221 agcagtttaa ttggaatttt gcttgtaatt tccagcttcc gttacgaaca ggctattgtg
     8281 ctacctaaaa gaaataggga tgcctcagta ctcgttacat catcaattct ttttagttta
     8341 gtttttagct ttctgctgtt cattgctatt atcctatttc gaaaggggat aggagaggcg
     8401 cttaacctag acgattctga tggatttatt ctttacctaa ttcctattgg ggtattcctt
     8461 ttgagtgttt ttcaggtgtt taacttctgg cttattcgcc agaaaggatt tatgaagatt
     8521 tcggttaata agctggttcg tagaattgtg gagggtggct cacagtcagc agcagccctt
     8581 actcattacc ccatgggtct tgttttagga gacgttgccg gtcaagttgg aaatgcagtt
     8641 acttcagcct accaatcggt aaggtgtggg tattctccaa ggcatttcag catagttcga
     8701 tctaaatatt tactcgatag ctattccgat tttccccgtt acagccttat tcctgcccta
     8761 tgcagcacta ttggattcct attgccggta gttctgatta acaggtacta cttgcccgaa
     8821 caggcaggtt tttttgatct ggctaaactt atcctatcgg ttccaatggc actagttgcc
     8881 acctcaattt cgagcgtagt gctacaaaaa gtttcggaaa gctatcgtct ggggaaatcc
     8941 tttgtgggag aacttaaacc actgatagct tttgttttta tagcttcagt agttgaggta
     9001 ctgctgattg tatttgcggg taagggcatt tttagggtgt tttttgggaa agtgtgggaa
     9061 acatcgggaa ttatttcgaa atggctggtt tggccttttg ccctgaactt tattacttca
     9121 tcatttacct ctatttttgt ggcgcttaac agaatcaggt ggcaaagctt gtggcaagtt
     9181 gcctacttcc tgctgattat ttcacttccg ctattcagaa atttcacgtt ttacggcttt
     9241 attagggttt atgctagttt ggaaatactt gccaatctgg caatggttac gctgctgact
     9301 attgtcgtta ttaaatacga gaaaaaattt tgaatgacat gatgaagcgt gtgcttttca
     9361 tagagccgat tgttgcccac taccgaaggg atacctaccg gttgatcttt agtgatagcc
     9421 gcttcgaatt tgcattgctg gcaggtgatt catacctgga tattaaacct gaaaaggttg
     9481 aaaaaaccat catatcaaca tataggaagt tcacgctact cggtgaggaa ttctactacc
     9541 tacgtaactc cctgcatcat gtgcgacact tcaacccgga tattgtggtt tgctccggag
     9601 tcgatttcca tcatctgcat accttactca ttcttctctg ggtaaaattt caccccaaaa
     9661 agaaatttat ctggtggtcg cacgccggat tggggcatca gggctggtgg ggacaaatgc
     9721 tacgaaaatg gttctactcc ttatctgatc atattcttgc ttatagcagc aaagggaagg
     9781 aaaaccttag gtgtatagaa atccctgaaa gtaaggttac cgtagttaga aactcgctaa
     9841 accaagaaga ttacgggttt gatgtggtta ggaatcttac agataatgag agcgtacagc
     9901 tgcttttttc gggaagaata tcgaggcaaa agggattaga cgtgctgatc agggcggtta
     9961 gcatacttaa taaaaatgga tattctttaa cttgtaagct agttggtagt ggtgatgatt
    10021 atgaatacag agaattagca aggagcttgg gcattggcaa cctagttgat tttgttgggc
    10081 ccctttatgg taaagccaat gaggaaattt tttcaagttc cggtattttt gttttccccg
    10141 ccggtattgg gctttcggtg gtgcatgctt tgtcctatgg cttaccggtt atcacaacta
    10201 ataatctaat atcacatggt cccgaattgg agctcattag gcagggtgtt aatggtgact
    10261 tttttgagga tggaaacccc tatgacctag cggctgttgt tgaaaaatgg attcacaagg
    10321 ttcagcgtta ttccattgaa attgcctctg cttgtcataa tagtgtggct gaacttgaat
    10381 atacgccgat aaaagtttct gaaaaaatat taaaagtctt atcctctttt tgattggagt
    10441 aaaatgcacc ctgaacttct taaaataaga acctttggac gaaattcact cctgaacttc
    10501 ttgtcgcgtt actcagagcc caaacctggc atccatatac tcaacagtca cttcttaagc
    10561 cttgatggca aagcttcgcc agaaattttc gaggagctac tttcgggtat ggaggatagg
    10621 ggtgtgaggt tcattagctt tgaggaggcg gttaaaagaa tcacagctta taatcttcct
    10681 gtggatgaat gcctggtggc cttctcctgg gatgatggct ttgaggagtg ctttacccga
    10741 attaagccgg ttttagatag gcgcagcctt aaagccgctt tttttattaa cccgggtttc
    10801 attgacggcg atgaagccta ccgctaccac tttacccatg aggtggttat ggtagataag
    10861 ccacccatga gctgggaaca gcttagaata ctaagttcag agggccatgt ggttggcgcc
    10921 cacacgctcg accatctatc gcttgattct gataataccg agttgctcca taggcagata
    10981 gctggctcaa aaattaggat agaggataaa atcaatgcta gggttgagca ttttgccttc
    11041 cccttcggaa ggctgaagct gctaagcccc ttaggacttc agcttgcact ctccaatttt
    11101 aagtatgttt acagccagag caactaccgt cattacttct ctttcgacgg aaaggttatt
    11161 aaccgtcgac acttcgagtg cgattggcct ttggagcatg ttatttactt tctaaaatct
    11221 aaaaagtttt gagggcaaag gttgacgtgc ttgtggtggg agattttcca cccagaactt
    11281 ttaccggcat tagcatggtt aatgccttag tgaagggaat gttggaggct aaagggaaaa
    11341 tagttgttgc tattgacgaa tcggcatgga ggttaaaagg gttaggtcgc atctttcatt
    11401 acttttcaag gtatgttgat gttgcccggc cgctggttag ctgtcgggtc gaaatccttt
    11461 acactaacat tcctctatca aggtttggat tactccgatt gattttgctt tgcttttttg
    11521 ctcgcataat ttcatggaaa acgacttttt acgggcatat ccacaggggg gatatacgtt
    11581 cattttttac atcatcccac gtaaacaggt ttcttttgaa gctatcattg agttttttta
    11641 acaggattat tgttcttagt aagcaatttg ccgatgaagt taaagcaatc aacagcaacc
    11701 aggtctttat tttgcccaat acctcgttac tcgaaggata tcgcaaaggc aggcaatatc
    11761 aatataatag aagtttcctt tgtgtctcca acattattag gagcaagggt ataggcgagc
    11821 tggttgaagc cttctcaagc ccggaactta ctgactttaa gttagctatt gttggaaata
    11881 tctccgagaa tgatttttac agggagctgg ttgatgtggc a
//
LOCUS       DAOPHX010000080        11864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_6449_length_11864_cov_6.734525, whole genome shotgun sequence.
ACCESSION   DAOPHX010000080 DAOPHX010000000
VERSION     DAOPHX010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 11864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11864
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6449_length_11864_cov_6.734525"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PK502_07060"
     CDS             complement(<1..891)
                     /locus_tag="PK502_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00612.1"
                     /translation="MGKTKFKNLFLAAVLLGGMDAFYNCDDDDNETLSRNEAEEMLTK
                     TDRGVVSITGEAMSSYGIKVMMWLNSLEMPEIPDKSVRVIPKLMFKSYEQQDRVLATI
                     LESSISPELAKAFDFDEAKNETNFEINDRVIRFSNDKRKVANPTDTIVSGKHKIVFIN
                     KDSFIPFAKQNNSFLFEPLTPKIELAYKGQVNSRKRYLAVAFDDFRVSDFSWLIPLFN
                     KYGFKTTFNRVNYGIPTDADFNKYSAVINGGHEIGDHTITHQMYPYYSPLFNGQNPVS
                     LDGTQQPFPTNADLRVNRGDG"
     gene            1063..1467
                     /locus_tag="PK502_07065"
     CDS             1063..1467
                     /locus_tag="PK502_07065"
                     /inference="COORDINATES: protein motif:HMM:NF025304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPH00613.1"
                     /translation="MVHLTQEQRYTISQMLGNGYKKIEIARVVGVDKSTVTRKIKRNA
                     DGRNGVYRYGLARHKAEDRRRTKPHAVVLTPEIVAHIERRLSHSMAARADFRMSEAGG
                     RWLACPPLPCLTKELHLQLKSTFHKIVKVRKN"
     gene            1604..2470
                     /gene="panC"
                     /locus_tag="PK502_07070"
     CDS             1604..2470
                     /gene="panC"
                     /locus_tag="PK502_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPH00614.1"
                     /translation="MKTIHTLKTCSEVEQSLWSTRENGKSIGFVPTMGALHGGHISLV
                     ERAASENDVVVVSIFVNPTQFNNPNDLVSYPRTPEKDLELLEKTGVDYVFMPSETEIY
                     PQPDTRTFNFGQLDKVMEGAHRPGHFNGVAQVVSRLFDIVKPNRAYFGQKDYQQLVII
                     RQLVRMLGLSIDIIACPIVREADGLAMSSRNLLLSPECRASAPLIHRTLVEACNQIEQ
                     MNVKDMIGWVEARVNQDKNLRVEYFEISNAETLEPVSSWTDAENIIGCIAVWAGSVRL
                     IDNIFLKHSTKG"
     gene            2473..2823
                     /locus_tag="PK502_07075"
     CDS             2473..2823
                     /locus_tag="PK502_07075"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HPH00615.1"
                     /translation="MLIEVVKSKIHRVTITEANLNYIGSITIDEDLMDAANLIENEKV
                     QVVNVNNGERLDTYIIKGERGSGQVCLNGPAARKCVTGDVVIIMSYATMDFEEAKHFS
                     PTVVFPDTATNKLV"
     gene            2841..3860
                     /locus_tag="PK502_07080"
     CDS             2841..3860
                     /locus_tag="PK502_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPH00616.1"
                     /translation="MKVFPSRRIVNFFIFFLLSILLLYLAFRKVDFSHVAQGFRNVSY
                     LWIFLSLLVGLASHALRAVRWSILVEPLGKKPSFLNMFSAVMVGYIANLALPRVGEVA
                     KCGSVSRTDGIRFESLVGTVVVERAMDLVMLLLATITVVALKFDFFGKFIISRIFHPL
                     RDRALQLNGYQLAAVLVVVVAFILFVRLILRSEIFGSKISLKIRSIWNGTVDGVKSIA
                     HTNKLGQFLVLSVVMWLCYWVMTWLLLYSTPITANLTVWDGLFIMVIGSYGMVVPVQG
                     GFGAYHIITAIALGIFGITYDDGLLFAIISHESQTFLVILGGILALIYVYIIQRPDKP
                     KPQSL"
     gene            4102..4578
                     /locus_tag="PK502_07085"
     CDS             4102..4578
                     /locus_tag="PK502_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HPH00617.1"
                     /translation="MNSEVIEKKILEDVELDRMLAYWKFRDRSVVALYGSFDVLHPGT
                     FELITYAAEHGHELVVGIKPDGEMKKEKGDKFPVFGQNKRALVVASNQLVSAVYICQN
                     GIEDFLRRVKPSLAVCCHHASSTDKDKLEVVKEWGGKYKIFTQSTTSVEDIITKLC"
     gene            4777..6174
                     /gene="radA"
                     /locus_tag="PK502_07090"
     CDS             4777..6174
                     /gene="radA"
                     /locus_tag="PK502_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPH00618.1"
                     /translation="MAKTKSVYVCQNCGAESVKWLGRCPSCGEWNTYVEERITKETSS
                     RPGLVNVSRNAIPTPIREIDSDNELRVDTKIGELNRILGGGIVPGSVILLGGEPGIGK
                     STLALQLALGLKSQKVLYISGEESARQVKLRADRINHDSQDCLILNETLLENILTQTE
                     RVCPELVVIDSIQTLYTDTIESSPGSVSQVRETAAALLRYAKTSGTPVILIGHITKDG
                     SIAGPKVLEHIVDVVLQFEGDQNYLYRILRSAKNRFGSTSEIGIFEMQSGGLVEVTNP
                     SEILLSHREEMLSGIAVAATIDGARPFLIETQALVSTAVYGTPQRSTTGFDPRRLNML
                     LAVLERRAGFRLANKDVFLNIAGGLRVNDPAIDLAIISAILSSTFDVAIAGSTCFAAE
                     VGLSGEIRPVSRLDQRIAEAAKLGMSRAFVSKYNLKKSDVHPKGIEVIPLGRIEELVK
                     ALFGRSSGRQQQADS"
     gene            6201..7472
                     /locus_tag="PK502_07095"
     CDS             6201..7472
                     /locus_tag="PK502_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00619.1"
                     /translation="MRIAIIGFGAAAIGIMERLKESQQEIHVFEKSADIYSSSISGIR
                     ADGKLFVSSEMGGDITIDTALQRKLVDYYINFTEKKNVETGTSFGSSRFYKVFYDRGF
                     LPVTSEFFHIGTDQLKEVLFNIYTHLSGVKNIHFHFNSTVSSIKIEKEGVLLNGTEHF
                     DMAVVTVGRSGHKLINRLIEDYPYLVKNNTKVDLGIRFELPNHIVEELNHEMYEFKVK
                     YKSRTGYMVRTFCNNPSGFVVTENYDDFTTVNGHAKLHEKSSNTNFAILASLILTEPF
                     NDPIGYGSNIAKLSNLLAGEGKVILQTYSHFKESKRTKNLYRVSPTLAADKFILGDIN
                     LAYPRRIIESIIDFIENLNTVIPGVANNDNLVYAPEIKFYSNRLNNLANPFIKFAGDC
                     SGATRSIIYATAHGYMMGEAILNNNFSTLES"
     gene            7472..9082
                     /locus_tag="PK502_07100"
     CDS             7472..9082
                     /locus_tag="PK502_07100"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPH00620.1"
                     /translation="MLKVDNLSVAFGQSMAVNGINFSLEGNQILGIVGESGSGKSVTS
                     LSIMGLLPKNARITNGSIWFEPPGCIPVNLVGINPEQHRQLRGKSIAMIFQEPHTCLN
                     PSMRCGVQLVEALELHAGLKGKPAVKQCLSLLSEMQLPDPETISRSYPHQLSGGQKQR
                     VMIAMALAGKPKLLIADEPTTALDVTVQKGILELLLSLRNRYGMGIIFISHDLSVVEQ
                     VADRVLVMRNGMMVEEGKAEDILQKPQHPYTRELIGFRREIYSPGNSSKTRNEGGGVV
                     FSVNSLTVRYEKSNRLSAGKAKNQIRAVSNVSFSIHEGETLGLVGESGSGKSTIGRTL
                     LRLIGNYSGQIAYKGKPLNGFTTQQLKEFRREVQLIFQDPYSSLNPRLTIGQALVEPV
                     VVHGLAGKNEAKSRVLELLDRVKLPADSFYRFPHEFSGGQRQRLVIARALTLQPKVLI
                     CDEIISALDIAIQVQILNLLEELKRDFNLTYLFISHDLGVVRHISNRVIVLKEGTIDE
                     EGPAEAIFAQPKSTYTKTLIAAMPQLLA"
     gene            complement(9135..11480)
                     /locus_tag="PK502_07105"
     CDS             complement(9135..11480)
                     /locus_tag="PK502_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPH00621.1"
                     /translation="MNWKNPEVIRKFKWWFWGIISFLLLFFLVIFILINLEFFGPMPT
                     FEELENPKSNIASEIISEDNVVLGNFYVQNRTFVGYDEISPNVIHALIATEDNRFYSH
                     SGIDAKGLGRVMVKTILFGKKTAGGGSTISQQLAKNLFPRDTSGRKNALVRTTKLVLS
                     KFKEWVTAVKLERNYTKEEILAMYLNVVEFGSNSYGIKSAAKTFFNTTPDSLHVEQAA
                     LLVGLVNAPTRYSPLRNPDKSLQRRNLVLQQMAKYGYIDRNQLDSLVAIPITLNYMEQ
                     DHNAGLATYFREMLRQFMTARKPERRFYYSQDMYSQDSLLWETNALFGWCSKNLKPDG
                     TPHNIYRDGLKIYTTINSKMQEYAEKSLTDHLKFELQPQLDREIKSRGGVLFSSQITR
                     EQANQIMYSAMRQTERYRLLRNAGASNESIIENFNTPVPMRVFSWRGERDTVLSPLDS
                     IKYYKKFLRSSFMAINPHDGHVKAYVGGPDFRHFKYDMVRQGKRQVGSTIKPFLYTLA
                     MQEGYSPCMEVPNVPQTFVVGDTTWNPKNSGSTKYDGQMVTLKWGLANSINNISAWVM
                     KQFTPQIVADMINQLGIKTHVDPVPSIFLGTIDFSLFEMVGAYGTFANKGIFIDPIFV
                     TRIEDRNGNVLATFNTVKTEAISEQTAYLMINLLEAVVNQGTGIRIRYKYQLPGKIAG
                     KTGTSQNHSDGWFMGVTPNLVAGVWTGAEDRAVHFDNISMGQGASMALPIFATFLQKV
                     YANPSLGVSPNDDWDKPILKQNVNLDCNKQFDNEINEIELY"
     gene            11662..11844
                     /locus_tag="PK502_07110"
                     /pseudo
     CDS             11662..11844
                     /locus_tag="PK502_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         3227 a         2726 c         2827 g         3084 t
ORIGIN      
        1 cccgtcccct ctgtttactc ttaaatctgc attggttgga aatggttgtt gtgttccatc
       61 taatgaaacc ggattttgtc cattaaataa aggcgaataa tacggataca tttgatgtgt
      121 aattgtatgg tctcctattt catgccctcc atttataacc gctgaatatt tattgaaatc
      181 cgcatccgtg ggaatcccgt aattaacccg attgaatgtt gttttaaaac cgtatttgtt
      241 gaataatgga attaaccagc taaaatcact aacccgaaaa tcatcaaagg cgacggctaa
      301 atatcttttg cggctgttta cttgtccttt atatgccaat tcaatttttg gggttaatgg
      361 ctcaaataaa aatgaattat tttgttttgc aaatggtata aatgaatctt tattaataaa
      421 aacaatttta tgttttcctg aaacaattgt atcagttggg tttgctactt ttcttttgtc
      481 atttgaaaaa cgaataaccc tgtcattaat ttcaaaattt gtttcgtttt tcgcttcgtc
      541 aaaatcaaag gctttagcca gctcaggaga aatgctagac tctaaaattg tggctaaaac
      601 tcggtcctgc tgctcatatg atttaaacat aagcttgggg attactctaa ctgacttatc
      661 tggtatttct ggcatttcca aggagttaag ccacatcatc accttaatgc cataactact
      721 catagcttcg ccggtaatgg agacaactcc cctgtcggtt ttggtaagca tttcttccgc
      781 ctcatttctg gaaagcgttt cattatcatc gtcatcacag ttgtaaaaag catccatacc
      841 gcctagcagc acagcagcta aaaatagatt tttaaacttt gtttttccca tgttccttaa
      901 attttcatta caaatgcaac tttttggggc gttagcttaa ggggcgaacg aaaaaactaa
      961 cctttcaaga acgagagtga aagggctagt ctctccctcg tcgttcctga cggttaggtg
     1021 gctatccttg ccccctaaat ctccccagaa ataagcggca gggtggtaca cttaacgcaa
     1081 gaacaaaggt acactatttc gcagatgttg ggaaacgggt acaaaaaaat tgagatagcc
     1141 agggtcgttg gggttgacaa gagcaccgtt acccggaaaa ttaaacgtaa cgccgacggg
     1201 cgcaatggcg tttaccggta tgggttagcc cggcacaagg ccgaggatcg caggcggaca
     1261 aagccacatg cggtggtgct cacccccgaa atagtagccc acattgagcg gcggcttagc
     1321 cattcgatgg ctgcccgagc agacttcaga atgagcgaag ctggagggcg ttggcttgca
     1381 tgtccaccac taccatgttt aacaaaagag ttgcatttgc agcttaaatc taccttccat
     1441 aaaattgtta aggtacgaaa aaattgaaaa aggaatattc ttttctgttg atttccaaca
     1501 agttgttttg cttcttttaa aggaaagatg tgccggaata gtttggagaa gcaaaaaaaa
     1561 tggcaaactt tgctgccgga attcaccaaa gttgaccaat caaatgaaaa caatccatac
     1621 gctaaagacc tgttctgaag ttgagcagag cctttggagc accagggaaa atggcaaatc
     1681 aatcgggttt gtaccaacca tgggggcgct tcatggggga cacatatcgc tggtggaacg
     1741 cgcagcctcc gaaaatgatg tggtggtggt aagcattttc gtcaacccca cgcagttcaa
     1801 caatccaaac gacctggtaa gctacccgcg aactccagaa aaggatttag aactacttga
     1861 gaaaacgggg gttgattacg tgtttatgcc ctccgagact gaaatctacc cccaacccga
     1921 tacccgcacg tttaacttcg gacaactcga taaggtgatg gagggcgctc acaggcctgg
     1981 ccacttcaat ggagttgcac aggtggttag caggcttttc gatattgtga agccaaaccg
     2041 ggcttacttt gggcaaaagg attaccagca gctggtcatc atcaggcagc tggttagaat
     2101 gctgggactc agcattgaca ttattgcatg ccccatagtc cgtgaagccg acggcttggc
     2161 catgagcagt cgtaaccttc ttctgtcacc agaatgcagg gctagcgcac cgttaattca
     2221 cagaaccctt gttgaggcct gtaaccaaat tgagcaaatg aacgttaaag atatgattgg
     2281 gtgggtagaa gcacgggtaa accaggataa aaaccttagg gtggagtact ttgagatttc
     2341 aaatgccgaa acactggagc ccgtaagcag ctggactgac gctgagaaca ttattggatg
     2401 tattgctgtt tgggcgggta gcgttaggct gatagataac atttttctca aacactcaac
     2461 aaaggggtag ctatgttgat agaagtagta aaatcgaaaa ttcaccgggt aaccataacc
     2521 gaggcaaacc tgaactatat tgggagcata accattgatg aggacttaat ggatgctgca
     2581 aacctgatcg aaaacgaaaa ggtacaggtg gtaaacgtga acaacgggga gcggctcgac
     2641 acgtatatta ttaaaggcga aaggggatcg gggcaggtgt gcctgaacgg ccctgcggcc
     2701 cgcaagtgcg ttaccggcga tgtggttatc attatgtcgt acgccaccat ggattttgag
     2761 gaagccaagc acttttctcc taccgttgtg tttcccgata ctgcaactaa taagcttgtt
     2821 taacacttaa cccgttcgcc ttgaaagttt tcccttcccg tcgaatcgtt aactttttca
     2881 tcttctttct actctccata ttgctgctct accttgcctt ccgcaaggtt gacttttccc
     2941 atgttgcaca gggatttaga aacgtaagct acttatggat tttcctttcg ctactggttg
     3001 gattggcatc ccatgcgcta cgggctgtcc gttggagcat tcttgttgaa cctcttggga
     3061 aaaaaccttc attcctgaac atgtttagcg ccgtgatggt ggggtatatt gcaaacctag
     3121 cactcccacg ggtaggggaa gtagcaaaat gcggaagtgt tagcagaacc gatggtattc
     3181 gctttgaatc gttggttgga acggtggtgg tggaacgcgc aatggacctg gtaatgctgc
     3241 tgctagcaac gattacggtg gtagcgttga aatttgattt ttttggaaag tttatcattt
     3301 cacgaatttt tcacccgtta agggatcggg cgctccagct caacggttac cagctggctg
     3361 cagttctggt tgttgtggtt gccttcatac ttttcgtcag gctaattcta cggagtgaaa
     3421 tttttggcag taaaatcagc ctcaaaattc gatccatctg gaacggcacc gttgatggag
     3481 ttaaatcaat agcccacacc aacaagctgg gccaattcct tgtactttcg gtagtcatgt
     3541 ggctttgcta ctgggtaatg acatggttgc tgctttactc aacccccata actgcaaatc
     3601 ttaccgtatg ggacggtcta ttcataatgg ttataggctc gtatggcatg gtagttccag
     3661 tacagggagg atttggagca taccatatca ttaccgccat tgcccttggt atctttggaa
     3721 ttacctacga cgacgggttg ctgtttgcca ttatttcaca cgaatcacaa acctttcttg
     3781 ttatactggg cggcatcctg gccttaatct acgtttacat aattcaaagg ccggacaaac
     3841 caaaacccca atccctgtaa agtcattagt aggcaagaag cttatagcaa cacgatgcca
     3901 ggttgactat tagcattact ttacctttac tggcgatgct aaactttttt gaaaatccaa
     3961 ttttttagag aatcctgctc cccctgacca tagcagattt taatatacct aaaactaacg
     4021 tcatcatgct aaaatttgct atgtttgtac aaatacaaca ttggctaaga gtgaatcata
     4081 cacagctaaa accataccca gatgaacagc gaagtgatag aaaagaaaat ccttgaggat
     4141 gtggagcttg accgtatgct tgcctactgg aagtttagag atagaagcgt ggtggccctt
     4201 tacgggagtt ttgacgtgct tcatcccgga acatttgagc ttattaccta tgctgccgag
     4261 cacgggcatg agctggttgt tggcattaaa cccgatgggg agatgaaaaa ggaaaaaggg
     4321 gataagttcc ctgttttcgg gcagaacaaa agagcgttgg tagtagcctc caaccagctg
     4381 gtttctgcag tttatatttg ccaaaacggc attgaagatt ttctccgaag ggtaaaacct
     4441 tcgcttgcag tatgttgcca tcatgcatcc tccaccgata aggataaact tgaagtagta
     4501 aaggagtggg gtggtaagta caaaattttc acacaaagca ccaccagcgt tgaggatatc
     4561 atcaccaagc tatgctagat tgtaaacgtg gcatagcacc cattcatggt aagccagctt
     4621 ttgcgtggcg gatggggaat tggtatttaa gatggatatt ggacttatag gtgcatgata
     4681 tagctatcat taaatctact ttttaggcgc tagctcttca acattgttaa caattaattg
     4741 cttgcgagat aacgtgcgct ataagctagg cgagtaatgg caaagacaaa aagtgtttac
     4801 gtttgtcaga actgcggggc agaatcggtg aagtggcttg gccgctgccc ctcatgtgga
     4861 gagtggaata cctatgtgga ggaaagaatt accaaggaaa cctccagcag gccagggctg
     4921 gtaaacgtat cacggaacgc tatccctact cccatcaggg aaattgattc ggacaacgag
     4981 ttacgggttg acaccaaaat tggagagcta aaccgcatac tgggcggggg aattgtaccc
     5041 ggttcggtta ttctgctggg cggggagcct ggtattggaa aatcaacact cgcccttcag
     5101 ctggcactag gcctaaaaag tcaaaaagtg ctctacatat cgggggagga aagcgcccgc
     5161 caggttaagc tacgcgccga ccgtattaat cacgacagcc aggactgcct tatactcaac
     5221 gaaacgctgc tggaaaatat actcacccaa accgaaaggg tatgccccga gttggtggta
     5281 atagactcca ttcaaacact ctacaccgat accattgaat cgtcgccagg cagtgtttcg
     5341 caggtgcgtg aaacggcggc tgccctgctc agatatgcaa aaacatcggg gactccggtt
     5401 atccttatag gccacatcac caaggatggg agcattgccg gccctaaggt actggagcat
     5461 attgtggatg tggtgctgca gtttgaaggc gaccagaact acctttaccg cattctccgc
     5521 tcggccaaaa atcgttttgg gtcaacctcc gaaattggaa tattcgaaat gcagagtggt
     5581 ggacttgttg aagttaccaa cccatcggaa attttgctgt cgcaccgcga ggaaatgctt
     5641 agcggcattg cggttgcagc taccatcgac ggggcaaggc ctttcctaat tgaaacccag
     5701 gccctggtta gcacggcagt ttacggaacc ccccaacgtt caaccacagg tttcgacccc
     5761 cgcaggttga acatgctgct ggctgtgctg gagcgccggg caggtttcag gcttgctaac
     5821 aaggatgttt ttctgaacat tgccggtgga cttagggtta acgatcctgc catagacctg
     5881 gccatcatat cggccattct ctcctccacc ttcgatgtgg caattgccgg ttcaacctgt
     5941 tttgccgctg aggtaggcct gtcaggcgag ataaggccgg ttagccgtct cgaccagaga
     6001 attgccgagg ctgccaagct gggtatgagc agggcttttg tatcaaagta taaccttaag
     6061 aaatccgatg tccaccccaa gggaatagag gttattcctt taggacgaat tgaagagctg
     6121 gttaaggctc tttttggtcg atcgtcaggc cgtcagcagc aggctgatag ctaaacatgt
     6181 tgaaattaaa tttttaagcc atgaggatag ccattatcgg gtttggtgct gctgccatcg
     6241 gcattatgga aaggctgaag gagagccagc aagaaatcca cgtgtttgag aaaagcgccg
     6301 atatctactc atctagcatt tcgggcatca gggcagatgg aaagcttttc gtctcatccg
     6361 aaatgggagg cgacataacc attgacaccg cccttcagcg taagctggtt gattactaca
     6421 tcaactttac cgagaaaaaa aatgttgaaa ccggtacttc atttggctcg tcaagattct
     6481 ataaagtatt ctacgacagg ggatttttgc ccgttacctc ggagtttttc catattggca
     6541 ccgaccagct caaggaggtg ctattcaaca tttacaccca tcttagcggg gtaaagaata
     6601 tccacttcca cttcaacagc acggtaagca gcattaaaat tgaaaaggag ggcgtactcc
     6661 tgaacggaac cgaacacttc gacatggcgg ttgtaacggt gggccgaagc ggtcacaagc
     6721 tcattaacag gctgattgag gattacccct acctggtaaa gaacaacacc aaggttgatt
     6781 tgggcatacg ttttgagctg cccaaccaca ttgttgagga gctaaatcac gagatgtacg
     6841 agtttaaggt gaagtacaag agccgaacag gctacatggt aagaactttt tgcaacaatc
     6901 cctcggggtt tgtggtaacc gaaaactacg acgatttcac aactgtaaat ggccatgcca
     6961 aactgcacga gaagagcagc aacaccaact tcgccattct cgcctcgctc atcctaaccg
     7021 aaccattcaa cgaccccata ggctacggat cgaacattgc caaactctcg aacctgcttg
     7081 ccggcgaagg taaggtgata ctgcaaacct acagccattt taaggagtcg aaacgcacaa
     7141 aaaacctcta cagggtgtct cctacgctgg cagcagataa gtttatcctg ggcgacatca
     7201 acctggccta cccccgacgg ataattgaga gcattattga cttcatagaa aatctcaata
     7261 cggtcattcc gggagtagcc aacaacgata acctggtgta tgcgcccgaa attaaatttt
     7321 actccaaccg gcttaacaac ctggctaacc catttataaa gtttgccggt gactgctcgg
     7381 gcgctacccg atccattatt tacgccactg ctcatggata catgatgggt gaggccattc
     7441 tgaacaacaa cttctcaacc ctcgaatctt aatgctgaag gttgacaacc tgagcgttgc
     7501 ctttgggcaa agcatggccg tgaacggtat caacttctcc ctggagggca accagattct
     7561 tggaattgtt ggggaatcgg gttcgggcaa atcggttacc tcgctctcca tcatggggct
     7621 gctgcccaaa aatgcccgca ttacaaacgg aagcatttgg tttgagccac ccggctgcat
     7681 tcctgtaaac ctggttggaa ttaatcccga acagcaccga cagctaaggg gcaagtcgat
     7741 tgccatgata tttcaggagc cccacacctg cctcaatccc tccatgcgct gtggggttca
     7801 gctagtggag gcccttgaac ttcatgccgg tttgaagggg aaaccggccg tgaagcaatg
     7861 cctttcgctg ctctcggaaa tgcagctacc cgatcccgaa acaatttctc gaagctaccc
     7921 ccaccagctt agtggaggac aaaagcaaag ggtgatgatt gccatggccc tggcaggcaa
     7981 acctaaacta cttatagccg acgagccaac aaccgctctc gacgttaccg ttcaaaaggg
     8041 aatccttgaa cttttactaa gcctgcgcaa ccggtatggc atgggtataa ttttcatctc
     8101 gcacgattta agcgtggttg aacaggttgc cgacagggtt ctggtaatga gaaacggcat
     8161 gatggttgag gaaggaaaag ctgaggacat cctgcagaag ccacaacatc cctacaccag
     8221 ggagctgatt ggtttccgta gagaaattta ctctcccgga aattcttcta aaacgaggaa
     8281 tgaaggaggt ggcgtagtat tctcggttaa ctcgctaact gttcgatacg aaaagagtaa
     8341 caggctatcg gcaggtaagg cgaaaaacca gattagggcg gtaagcaatg tgagcttctc
     8401 cattcatgaa ggcgaaacat tggggctggt gggcgagtcg gggtcgggta aaagcaccat
     8461 tggccgaaca cttcttaggc taattggaaa ctactccggc caaatagctt acaagggtaa
     8521 accacttaac ggcttcacca cacagcagct aaaggaattt cgccgggagg ttcagctgat
     8581 ttttcaggat ccctactcct cgttaaaccc acgcttaaca attggacaag ctcttgtaga
     8641 acctgttgta gtccatgggc tggctggcaa gaacgaagca aaatcacgcg tgctggagtt
     8701 gctggacagg gtgaagctac ctgctgatag cttctaccgt tttccacacg aattctcagg
     8761 tggtcagaga caacggctgg taattgcccg cgcgcttacc cttcaaccaa aggtgttgat
     8821 ttgcgatgag attatttccg cccttgacat tgccatacag gttcaaattc tgaacctcct
     8881 cgaagagcta aaacgggact tcaacctaac ctaccttttc atctcgcacg atttgggggt
     8941 agttaggcat atcagcaacc gggtgatagt cctaaaggag ggaacaattg acgaggaagg
     9001 ccctgctgag gcgatttttg cacagcccaa aagcacctac acaaaaacgc tcattgcggc
     9061 catgccgcag ctactagcct gatgtaaccc gaaacgatca agcccaaacc tgctaccatg
     9121 cacgaagcaa aaggctagta cagctcaatt tcgttaatct cattatcaaa ctgcttattg
     9181 cagtcgaggt taacgttttg ctttaggatg ggtttatccc aatcatcgtt gggcgaaact
     9241 ccaagcgatg gatttgcata taccttctgc aaaaaagtag caaaaattgg caaagccatg
     9301 ctggcgccct gccccatgga aatattgtca aaatgaacag ccctgtcctc ggcgccggtc
     9361 caaacgccag caaccaggtt gggagtaaca cccatgaacc atccgtcgga gtgattttgg
     9421 gatgtccccg ttttcccggc gatttttccg ggcagctggt acttgtagcg tatgcgaata
     9481 ccggtgccct ggttaacaac agcttcgagc aggttgatca tcaggtaggc tgtttgctcg
     9541 cttatagcct cggttttcac cgtgttaaag gtagccagca catttccatt cctatcctcg
     9601 atgcgggtta caaaaattgg atcgataaaa atgcccttgt tggcaaaagt gccgtaggcc
     9661 cctaccatct cgaaaaggct aaagtcaatg gtgccaagaa aaattgaagg aaccggatca
     9721 acgtgagttt taatacccag ctggttaatc atgtctgcaa ctatttgcgg ggtaaactgc
     9781 ttcataaccc aggccgaaat gttgttgatg gagttggcca aaccccactt aagggttacc
     9841 atctgcccgt cgtacttagt actgccggaa tttttggggt tccatgttgt atcgcctacc
     9901 acaaaagttt ggggaacatt tggaacctcc atgcatgggg aatacccctc ctgcatggca
     9961 agtgtgtaaa gaaaaggctt aatggtggag ccaacctgtc ttttcccctg cctaaccatg
    10021 tcgtacttaa agtgtcgaaa atcagggcca cccacgtatg ccttcacatg accatcgtgg
    10081 gggttaatgg ccataaaact cgaccgaaga aactttttgt agtacttgat ggagtcgagg
    10141 ggtgataaaa ccgtatcgcg ctcaccccgc catgagaaaa ccctcatcgg aacgggggtg
    10201 ttgaagtttt cgataatact ctcgtttgaa gctccggcat tacgaagcag cctataacgc
    10261 tcggtctgcc tcatggctga gtacataatt tggttggcct gctcacgggt aatctgtgag
    10321 gaaaacagaa ccccacccct actttttatt tcacgatcga gctggggttg cagctcaaac
    10381 ttcaggtggt cggtaagcga cttttctgcg tactcctgca ttttggagtt gatggtggtg
    10441 taaattttaa ggccatcacg gtagatgttg tggggtgttc catcgggctt aaggtttttg
    10501 gagcaccatc cgaaaagggc attggtttcc caaagtaggg aatcctggct gtacatatcc
    10561 tgagagtagt aaaatcgcct ttcgggttta cgggcggtca taaactgcct gagcatttcc
    10621 ctgaagtacg tcgccaaacc cgcattgtgg tcctgctcca tgtagttcaa ggtgattgga
    10681 attgcaacca gggaatcgag ctggttgcga tcaatgtacc catactttgc catctgctgc
    10741 agcaccaggt ttcgtcgctg taacgactta tcgggattcc gcagggggct ataccgggtt
    10801 ggagcattta ccaatcccac cagcagggct gcttgctcaa catgaagaga atcaggagtg
    10861 gtgttgaaaa atgttttagc tgccgattta atcccatagg agttgctgcc aaactcaacc
    10921 acgttcaggt acatggccag aatttcctcc ttggtgtagt ttcgctccag cttaacggcc
    10981 gtaacccact ccttaaactt cgacaatacc agcttggtgg ttcttaccag cgcatttttt
    11041 cgccccgagg tgtcgcgggg gaacaagttt ttggcaagct gctggctaat agtgcttccc
    11101 cctccggcgg tctttttccc aaatagaatg gttttaacca tcacccttcc caaacccttt
    11161 gcatctattc cgctatggga gtagaaacgg ttatcctcgg ttgcaatcag cgcatggatt
    11221 acattaggtg atatttcgtc gtagccgaca aacgtcctgt tttgaacgta gaaattaccc
    11281 agcaccacgt tatcctcgga tatgatttcc gaggcaatgt tgcttttcgg attctccagc
    11341 tcctcgaatg tgggcatggg accaaaaaat tcgaggttga tgagtatgaa aatcaccagg
    11401 aaaaaaagta gcaggaatga gataattccc caaaaccacc atttaaactt tcggattacc
    11461 tccggatttt tccaattcat gggcttaaaa atttagcatc acgcgctccc tataaacata
    11521 aactttaacc attcgcccct taggtcaact ttcctttgtg ccagcaagtg aaaacgtgca
    11581 acgttcacct ggtagtaaaa cgaacaaagt taaaaactat tttggacata gctcacaacc
    11641 aggcaaccgg tggtaaggat tgataggctg atttcgaaaa caagagtgga gcatgttttc
    11701 ggttttatgg aacaaagtat gaatggtata acggtgcgtt ctgttggtat tgtcatagcg
    11761 acaggcatta tcgggctaat aaatttgaca tataatttat tccgatacga acaagttgtt
    11821 cggttaaata ttttacaaag ttaagtagtt gatagataga taga
//
LOCUS       DAOPHX010000081        11638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_6629_length_11638_cov_5.855133, whole genome shotgun sequence.
ACCESSION   DAOPHX010000081 DAOPHX010000000
VERSION     DAOPHX010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 11638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11638
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6629_length_11638_cov_5.855133"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..522)
                     /locus_tag="PK502_07115"
     CDS             complement(223..522)
                     /locus_tag="PK502_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00622.1"
                     /translation="MKRVELDFLFILPFIPVLLPAQVTTDDYMRAEKFLQANLGKHIY
                     NSWVEPSWQKDGCTFVYSVNTRKGKEFFRVDAKVGVKIPAFNHAKLASLSPQANR"
     gene            complement(572..2935)
                     /locus_tag="PK502_07120"
     CDS             complement(572..2935)
                     /locus_tag="PK502_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HPH00623.1"
                     /translation="MNQRIKTLREQSLKAVNTLSAERALLITKYYSSQQVEGCPTPIL
                     RAQAFDYLMSHKSICINPGELIVGERGPAPKAAPTYPEVCLHSLDDLQLISTRPKVSF
                     RVDEETFRVYRDTIIPFWRGKTLRDMIFSSVSEEWRKAYDAGVFTEFQEQRAPGHTVA
                     GKKVFQKGMLDLIDDVDASLNKLTKDNNGCTKAKIEELEAMRIAANAIIKFANRYADE
                     LERLAKIEVDANRRVELGEMAAICRRVPAHAPQTFHEALQHYWFIHLGVITELNPWDS
                     FNPGRLDQHLYPFYLNDIKEGKLTHEGAVELLQAFWVKFNNHPSPPKMGVTAEESNTY
                     TDFTLVNLGGVKEDGSDAVNELTYTILDVIEEMRILQPSSMVQVSKKNPDRFVKRAVK
                     IVKTGFGQPSFFNSDAIVQELLSQGKSLTDARNGGASGCVETGAFGTEAYILTGYFNL
                     NKVLELTLNNGVDPLTGKEIGIRTGNAASFKTFNQLYEAFERQLGYFSDVKLEGNLVI
                     ERIWAEEMPVPFLSLLIDDCIANATDYNAGGARYNTSYVQGVGLGSITDNLSALSRLV
                     FDEEALSMDELFKAMNANFEGYDDLRYRLIYDVPKWGNNNDYADKLAVRVFNSFYKAI
                     TGRKTFRGGVFRINMLPTTSHVYFGSKIGAMPDGRLAHEPLSEGISPVQGADRNGPTA
                     VILSAAKIDQIKTGGTLLNQKFSPSFFTTDESLDKMVSLIRSYFKLDGHHIQFNVVSA
                     DTLRDAQKHPEKYQDLIVRVAGYSDYFNDLGEALQNEIIRRTEHEAV"
     gene            complement(2977..3780)
                     /locus_tag="PK502_07125"
     CDS             complement(2977..3780)
                     /locus_tag="PK502_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPH00624.1"
                     /translation="MIATKGLVFDIRHFSVHDGPGIRTAVFLKGCPLRCMWCHNPESQ
                     SLLPENMKRSRKVGEKSFDFDEMVGREMTPNEVVAELQKDIPIYDESGGGATFSGGEP
                     MIQFDFLYQVLEECRRMEIKTAIDTCGHAPLEQFRAVEPLTNLFLYDLKLIDSTQHKE
                     FTGVDNSLILQNLEMLLSRKARIHIRIPLIPGITDTDENLLGIKDFLLSYSGAERIDL
                     LPYHDIARSKYVRLGRDNPFKLSGAYDSQKLSRIQDLFIDLNIPVTVGG"
     gene            complement(4209..4418)
                     /locus_tag="PK502_07130"
     CDS             complement(4209..4418)
                     /locus_tag="PK502_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00625.1"
                     /translation="MGLDIKLPIGLMFTIFGIILTVYGIFTNSDPQMYQKSFLVNINL
                     WSGIGMLIFGIFMLALTKKKKSEKK"
     gene            complement(4439..6169)
                     /locus_tag="PK502_07135"
     CDS             complement(4439..6169)
                     /locus_tag="PK502_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPH00626.1"
                     /translation="MEMSFDLKWIDYLVMIIYFVFVLGIGWALKKFMKDANAFLEAGR
                     SMPAWVAGLAFISTNLGALEVMGMTGSGAKYGMLTTHFYWVGAIPAMVFLAMFMMPFY
                     YGSKARSVPEYLKLRYDEKTRGLNAILFALMTVLSSGISMYALAILTEKVLGWNFHVS
                     LLLSAAIVLGYTYLGGLSSAIYNEVLQFFIIVIGLLPLVFLSLHQVGGWEGLQSQLAP
                     VAASHGYEPDAYTTMWRHSGTAGGNPLGVEWYGILLGLGFVLSFGYWCTNFLIVQRAM
                     AAESPRAARNTPLIGAIPKMFIPFLIIVPGMVALALMNNPAAQFTIPTNEAGQPIYDY
                     TIISLLKYYLPSGALGLGITALLASFMSGMAGNISAFNTVWTYDIYQSYIRPATANKE
                     ADDKHYLKVGRLATIFGVLVSIAAAYLASMFGNIMDFLQTIFSMINAPLFAVIFLGMF
                     WKRSTGHAAFTGLLAGFLIALLHHGLTVPAGATTLVKGGWIATLHVYPVEMAQNFWTA
                     IFAFTTSALVTVVISLLTRRTKSNDELVGLVYSLTPRMKEDHVVWYKRSSTLAVVVLL
                     IATILTILFW"
     gene            complement(6829..8982)
                     /locus_tag="PK502_07140"
     CDS             complement(6829..8982)
                     /locus_tag="PK502_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPH00627.1"
                     /translation="MEIQEMAAQHVLLKSWSYQWKPLFRGYTDKTLYINVGNNEVMEK
                     PVPPEVKEKFIGGKGYDLRLLWDATKPTTKWNDPENEIVISSGPIGGITQYSGTGKSI
                     VCTISPQTDIVIDSNVGGFFGPFLKFSGFDAIEIQGKAKRDVIIYIDGITHKVELFEA
                     PAEPIDSHVLGEVLSEMFANDEADKKNIGIVSTGVAAEHSLFGMLNFTFYDPKRKCIR
                     LKQAGRGGIGTVFRDKKIKAIVCKIPGVKGNLNNVADLEAIQERGRRFNKEIRELDDK
                     QCQMRQKGTAHLVEIMNDYDLLPTHNFQYGSHKEAVKIHSEVWKSYFTQGVPDGCWIG
                     CNMACAKGVDKFKLKTGPYKGQEVIVDGPEYETAAGLGSNGGMFDPEYVIEANFYCDT
                     YGICTITWGTTLAFILECYQRGVLNKERTGGLDLTWGNTSDALELLHQLARGEGFGKI
                     AGLGVHKLKEYFIREFGADPQLMQDIGMENKGLEYSQYVSKESLAQQGGYAMTNKGPQ
                     HDEAWLIFMDMVNNQIPTFEAKAEALHYFPMFRTWFGLQGLCKLPWNDVEPANNAETD
                     EPAKVPEHVDNYVTIYNAVTGSKIDKHEIIRQSERVYNFQRIFNIRRGYGLRKNDAQP
                     YRAAGPVTREEYLSREERYDKQLKELVGVDPAKMTLEEKMAALRKYRENRYEQLLDAV
                     YKRRGWNKNGVPTIAHLKEIGMDLPELIEVVTPLQ"
     gene            complement(9161..10195)
                     /locus_tag="PK502_07145"
     CDS             complement(9161..10195)
                     /locus_tag="PK502_07145"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HPH00628.1"
                     /translation="MNLRICPWGNIHGKEVYLYTATTRNGMKCTISNYGGLIKSIALI
                     SEDNKELEVAYGFDSIESYREADYHPGGIIGRYANRIAGSEFTIDGHNHQLSCNEGSN
                     QLHGGINGFDRKIWVPEIVEARDGVCTIQLFLHSPDGDEGFPGNLDVWLTYSITEDNQ
                     IAISYKAATDKPTHVNLTTHGYFNLSGFRKNVLDHLLHLSADHYLPVDECLIPTGELR
                     NVSHTPFDFRTTKSIGADINRCGGFYDHCFALNNPTLDKPAAVLRNPDNGVCLKLFTT
                     QPGLQLYTPINRPNVSNPAIPFPESGNWAVCLEPQHFPNTPNMPDFPTTLLMPNGEYS
                     QTTIISFEQE"
     gene            complement(10229..10546)
                     /locus_tag="PK502_07150"
     CDS             complement(10229..10546)
                     /locus_tag="PK502_07150"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPH00629.1"
                     /translation="MSVESRKFWRWFLSKLKNKFFLTAVIFLLWMLIFDSSNWISMWE
                     TKRNIQRLENERDYYIQKIQDDRRKINELQTNNENLEKFAREQYLMKKPNEEIFIVDE
                     EDL"
     gene            complement(10550..10759)
                     /gene="thiS"
                     /locus_tag="PK502_07155"
     CDS             complement(10550..10759)
                     /gene="thiS"
                     /locus_tag="PK502_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HPH00630.1"
                     /translation="MDIILNGIPEHIDTTKVNITVDELLRFKKFSFKMLVVRINGELV
                     RKEQYSTATITNGDNVQVIHLISGG"
     gene            complement(10930..11538)
                     /gene="thiF"
                     /locus_tag="PK502_07160"
     CDS             complement(10930..11538)
                     /gene="thiF"
                     /locus_tag="PK502_07160"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HPH00631.1"
                     /translation="MPSAREVKEALRGYSVGIAGVGGLGSNVAMLLTRVGVGHLVIAD
                     YDTVTEDNLSRQYFFWHQVGWSKIRALRENLLAINPMVDVRGHELKVDEDSALGVFDG
                     VDVLVEAFDDARMKKVLIEGFQLNRPDVPIVAGSGIAGWGKSNDIRVVQYGNIYLCGD
                     GVSEVSDDLPPLCPRVSICASMQANVVVSILLGRFWGGEPQP"
BASE COUNT         3006 a         2853 c         2564 g         3215 t
ORIGIN      
        1 aggttttcca ttggggggaa atatacgcaa ttgtaccctc gctgccgcac ctctcccgtc
       61 gtgtggcaac cccatcccta gttctctgcc gcaacaacta cccccttatc cttaaattta
      121 tagcgcataa tctcgctggt attgttttac aacttgctac cactcacttt ccatttcttt
      181 tcttaactgc cacacggttg gcttcgccga gttatccgcc agctagcggt tcgcttgggg
      241 tgatagcgac gctagcttgg catggttgaa tgccggaatc tttaccccga cttttgcatc
      301 aaccctaaaa aactccttcc ctttccgggt gttcacgcta tacacaaaag tgcagccatc
      361 cttctgccac gacggttcca cccagctgtt gtagatatgt tttccgaggt ttgcctgaag
      421 aaacttctca gcccgcatgt agtcgtccgt ggttacctgt gccggtaata gtaccggaat
      481 gaatggcagt atgaataaga aatcaagttc tactcttttc atgatttaca gtgtttattg
      541 attttggcat agcccatcaa cacgtttgcc gttaaacggc ctcatgctcg gttcggcgga
      601 taatttcatt ctgaagggct tcgcccaaat cgttgaagta gtcggagtaa ccggcaaccc
      661 ttacaattag atcctgatac ttttcggggt gcttttgagc gtcgcgcagc gtatcggcgc
      721 taaccacgtt aaactgaatg tggtgaccgt cgagtttaaa gtagctccgt atgagcgata
      781 ccatcttgtc gaggctttcg tcggtagtga aaaacgatgg cgaaaacttt tggttaagca
      841 gcgttcctcc ggttttgatt tggtcaatct tagctgccga aagaataaca gcagttggtc
      901 cgtttctgtc tgctccctgc acggggctaa ttccctcgct gagaggttca tgagccaagc
      961 gtccatcggg cattgcccca attttgctgc caaagtaaac atggctggtg gtagggagca
     1021 tgtttattcg gaaaaccccg cccctgaaag ttttccgtcc ggtaatggcc ttgtagaagc
     1081 tgttgaaaac cctaacggcc aacttgtcgg cataatcgtt gttgttgccc catttgggca
     1141 catcgtaaat cagtctgtag cgaaggtcgt cgtagccctc aaagttggca ttcatggcct
     1201 taaacagctc atccatagat aaggcttcct catcgaacac cagcctgctc agagccgaca
     1261 ggttatcggt tatgctaccc agcccaaccc cctgcacgta gctggtgtta taccttgccc
     1321 caccggcatt atagtcggtt gcgttggcaa tacagtcgtc tattaggagc gagaggaatg
     1381 gaacaggcat ttcctctgcc caaatacgct caatgacaag gttcccctca agcttcacat
     1441 cggaaaagta accgagctgc ctttcaaaag cctcgtagag ctgattgaaa gttttgaatg
     1501 aagcggcatt gccggttctt attccgattt ccttacctgt aagcgggtca acaccattgt
     1561 tcagggttag ctcgagcacc ttgttcaggt tgaagtagcc tgttagaatg taggcttcgg
     1621 ttccaaaggc tcctgtttca acacaaccgc ttgctccgcc gtttcgagcg tcggtaagcg
     1681 atttgccctg gcttagcagt tcctgcacaa tggcatcgga gttaaaaaac gatggctgtc
     1741 caaaaccggt cttaactatt ttgactgcac gcttcacaaa cctgtcgggg tttttcttgc
     1801 taacctgcac catgctgctg ggttgtagta ttcgcatctc ctcaataaca tcaaggatgg
     1861 tgtaggtgag ctcgttaacc gcatcggagc cgtcctcctt aacgccacca aggttaacca
     1921 gggtaaaatc ggtgtaggtg ttgctttcct cagctgttac acccatcttg ggcggtgaag
     1981 gatggttgtt gaacttcacc cagaaggctt gcagcagctc aacagcacct tcatgggtaa
     2041 gcttgccctc tttgatatcg ttcaggtaga aagggtagag atgctggtca aggcgtccgg
     2101 ggttgaagga gtcccaagga tttaactcgg taattacacc aagatgaata aaccagtagt
     2161 gctgcagcgc ctcgtgaaat gtttgagggg catgggcagg cacccttcgg caaatggcgg
     2221 ccatttcacc aagctctact cgcctgttgg catcaacttc gatttttgcc aacctttcca
     2281 gctcatcggc ataacgattt gcaaatttta tgatagcatt agcagcaatc cgcatggctt
     2341 ccagttcttc tatctttgcc tttgtgcagc cattgttatc tttggtaagc ttgtttagtg
     2401 aagcatccac atcgtcaatt aaatcgagca ttcccttttg gaaaactttt ttaccggcaa
     2461 cagtgtggcc gggagctcgc tgttcctgaa actcggtgaa aacgcctgca tcgtatgcct
     2521 tacgccattc ttcactaact gacgaaaaaa tcatatcgcg cagggttttt cccctccaga
     2581 aagggattat ggtatcacgg taaaccctga aagtttcctc gtcaaccctg aacgatacct
     2641 ttggacgggt gcttatcagc tgcaaatcgt ctagcgaatg caggcatacc tcggggtagg
     2701 taggggcagc cttaggtgcc ggtccccgct ccccaacaat aagctcaccg gggttaatgc
     2761 aaatggactt atggctcatc aggtaatcaa atgcttgggc tcttaaaata ggggtggggc
     2821 agccctccac ctgctgcgaa gagtagtatt ttgttatcag caaagctcgt tcggccgaca
     2881 gggtattcac cgctttaagg ctttgctccc ttagtgtctt tattctttgg ttcataattg
     2941 taacatttaa ctatttaaaa taagtcaatg gacaaactag cctccaacag taaccgggat
     3001 gtttaaatcg atgaacaaat cctgtatccg gcttagcttt tgcgaatcgt aggcgcccga
     3061 aagcttaaat gggttatccc tgccaagcct aacgtacttg ctgcgcgcaa tgtcgtggta
     3121 gggtagcagg tcaatacgct ccgcaccgct ataggagagg aggaagtctt taatacccaa
     3181 caggttttcg tcggtatcgg taatgccagg aattagggga atacgaatat gaattctggc
     3241 cttcctggat aaaagcattt cgagattttg taaaattagc gaattgtcaa ctcctgtaaa
     3301 ttccttatgc tgggttgaat caataagctt taggtcgtag aggaacaggt tggtcaacgg
     3361 ttcaaccgct ctgaattgtt ccaatggagc atgtccacag gtatcaatcg ctgttttaat
     3421 ttccatcctg cggcactctt ccaaaacctg gtagagaaaa tcgaactgaa tcattggctc
     3481 gcctcccgaa aaagtagctc ctccaccaga ttcatcataa ataggaatat ctttttgcag
     3541 ctcggcaaca acctcattgg gggtcatctc gcgtcccacc atttcgtcga agtcaaagct
     3601 cttttcaccc accttacggc ttcgcttcat gttctcgggt agtaggctct ggctctcggg
     3661 gttgtggcac cacatgcagc ggagcggaca acccttaagg aatacggctg ttcttatgcc
     3721 cggaccgtcg tgtaccgaaa aatggcgaat gtcgaacacc aaccctttag tagcgatcat
     3781 gtcaaaatat tatgttagaa ttgtgatact ataaagaaca atacagagga taacactatc
     3841 ctttagggag aatgaatatg ggattagaaa attttcaaca aatccagcag gcaaagaggc
     3901 ctttgccatt attatggtag agggtggagg tattggattt gttgtacatg ataccgcaac
     3961 ttctaaacct atttttacaa aaatacgcta tttcaggata cctgtcaatg atatttgtca
     4021 tttattaaac atttagcttg aagcaattta ccccaccggt taatggttgc caatacaaat
     4081 tttagggcat aagtaagcta ccttagtaat gccaggcatg ttatggtagc ttattaaggt
     4141 aagacccaaa aaaagaagac cgctggtgct agaccaacag cctcctttga attagctaaa
     4201 gatgtccgct actttttttc ggattttttc tttttagtga gcgccagcat gaaaatgcca
     4261 aaaattagca ttccaatgcc cgaccatagg ttgatattta ccagaaagga tttttggtac
     4321 atctggggat cagaattcgt gaatattcca tacacggtaa gaattattcc aaaaatggtg
     4381 aacatcagcc caataggaag ttttatatct agtcccatga cacttattct ttaaatgatt
     4441 accagaacaa aatagtaaga atagttgcaa tcagcaaaac tacaacggca agagttgacg
     4501 agcgtttgta ccataccacg tgatcctcct tcattcttgg agtgagggag taaactagtc
     4561 ctaccagctc atcgttcgat ttggttcttc tggttagaag cgaaattaca acggtaacca
     4621 gagcgctagt ggtaaaggca aaaatagcag tccagaaatt ctgagccatt tctaccggat
     4681 agacgtgtaa tgtagctatc cagccacctt taaccagggt tgttgcaccg gcaggcacgg
     4741 ttaatccatg atgcagcagg gctatcagaa aacctgcaag caaccccgtg aaggcagcat
     4801 ggccagttga cctcttccag aacataccca agaagataac cgcaaagagc ggagcgttaa
     4861 tcatggagaa gatggtttga agaaaatcca tgatgtttcc aaacatgctg gccaggtagg
     4921 ctgctgctat gcttaccagt accccaaaaa tggtggctaa acgaccaact ttaaggtagt
     4981 gcttgtcgtc agcttccttg ttagcggtgg ctggacggat atagctttgg taaatatcgt
     5041 atgtccaaac cgtgttgaag gcggaaatat ttcctgccat tcccgacata aatgaagcca
     5101 gcagcgctgt aattccaagt cctaatgccc ctgagggtag gtagtatttc aacagcgaga
     5161 taatggtgta atcgtaaatt ggctgacctg cctcgtttgt aggaatggta aactgagcag
     5221 cagggttatt cataagcgcc agcgcaacca tgccgggaac gataatcagg aatggtataa
     5281 acattttagg gatggcacct ataaggggag tatttcttgc tgccctgggc gattcggcag
     5341 ccatggcccg ttgaacaatt aggaagttgg tgcaccagta cccaaacgaa agcacaaaac
     5401 ccaaccctag cagtattcca taccactcga ccccaagggg gtttcccccg gctgtccccg
     5461 agtgcctcca catggtggtg taggcatcag gctcatagcc atgactcgct gctacaggtg
     5521 ccagctggga ttgcaaaccc tcccaacctc ctacctggtg caagcttaaa aatactagcg
     5581 gtagcagacc aatcacaatg ataaaaaact gcagcacctc gttgtagatg gctgacgaca
     5641 aacctcccag ataggtgtag cccagcacaa tggctgccga caaaagcagg cttacatgga
     5701 aattccaccc caacactttt tcggttaaaa ttgccagcgc atacatggat atccccgagg
     5761 atagcacggt cataagggca aacagtatgg cattcaaccc ccttgttttt tcatcgtagc
     5821 gcagcttaag gtattccgga accgaacgcg cctttgaacc gtagtaaaaa ggcatcatga
     5881 acatcgccag aaataccatg gccggaatgg ccccaaccca gtaaaaatgg gtggtgagca
     5941 ttccatactt tgcgccgcta ccggtcatcc ccataacctc caatgccccg aggttggtag
     6001 atatgaatgc caaaccggct acccatgccg gcatagagcg tccggcctca agaaaggcgt
     6061 tggcatcctt cataaacttt tttaatgccc atcctatccc aaggacaaac acaaaataga
     6121 ttatcattac cagataatct atccacttaa gatcaaaact catctccatc gtttcagcag
     6181 tttttgttga acgtcgtgtt acgtactatt tatacctgat tatataagct attcatttct
     6241 tgtattttcc ctcgggtaaa aaaaccacat ccgattaact aaaaaacaga aagttaagcc
     6301 aatgcagacg gctatcgcta aatttttaaa tcaaaatcat tacgtcaagt acaaattaag
     6361 ctgtgtttgc ctgtcaaatt tagaggaaat ttaaacaaaa tttaacctga agtataattg
     6421 atgagctgcc ttggcaaggc attttaaaca gcaatagctg tgtaaacatt acacgcccat
     6481 tctctatcat gatgaaatcc tgatatttgg acacttcccg gagttgatgc aaggtaatgc
     6541 cagcagcgca gcccatccct cggggtgtag cccttgccag aaggtgaaaa tcctcatcat
     6601 agtcttcagt ttttagctga ttaatggctt attaaactca taagcagaga tatgacagat
     6661 tagctggagt atggaacaac attttttcaa aaattgatat tctatctggt tgtattttaa
     6721 agtgttaaca gtagtatggc aaacgtacgg gtaataaaaa tggggttaac ccgtaggcta
     6781 accccaaaac caactcacgc taacaacttg taatgtttac actgttgact actgcaaggg
     6841 agtaaccacc tcaatcaatt cgggcagatc catgcctatt tccttaagat gggctatagt
     6901 gggaacaccg tttttattcc atcccctacg tttgtaaacg gcatccagta gttgttcgta
     6961 gcgattttcg cggtactttc taagtgcggc cattttttct tcaagagtca tttttgcggg
     7021 gtcaacgcca accagctctt tgagttgctt gtcgtatcgc tcctcacgac tcaggtactc
     7081 ttcccgggta accggaccgg cagccctgta gggttgggcg tcgtttttac gaaggccgta
     7141 gccccgacgg atgttgaata tacgttgaaa gttgtacacc cgttcgctct gccttattat
     7201 ctcatgctta tcaattttac tcccggttac ggcattgtag atggtaacat agttgtcaac
     7261 atgctctgga accttagcag gttcatccgt ttcggcatta ttggcaggtt caacgtcatt
     7321 ccacgggagc ttgcatagcc cctgcaatcc aaaccaggtt ctgaacatcg ggaagtagtg
     7381 gagggcctcg gcctttgcct caaatgtggg gatttggttg ttcaccatat ccataaaaat
     7441 cagccaggct tcatcgtgct ggggtccctt atttgtcatt gcgtagccgc cctgctgggc
     7501 tagcgactcc ttcgaaacat actggctata ctccaacccc ttgttctcca taccaatatc
     7561 ctgcatcagc tgaggatcgg cgccaaactc cctgataaag tactccttta gcttatgaac
     7621 tcccaagcca gcaattttgc caaaaccctc accgcgggct agctggtgaa gaagttccag
     7681 cgcatcgctg gtgtttcccc atgttaaatc gagtcctcct gtacgctcct tattaagaac
     7741 ccctcgctgg tagcattcca gaataaaggc cagcgtggtg ccccaggtaa tagtgcaaat
     7801 gccataggtg tcgcagtaaa agtttgcctc aatcacgtac tccggatcaa acattccgcc
     7861 atttgatccc aagccagctg ctgtttcgta ttcaggcccg tcaacaatta cctcctgtcc
     7921 tttatagggt ccggttttta gcttgaactt atccacgccc ttggcacatg ccatgttgca
     7981 gccaatccag cacccatcgg gaacaccctg ggtaaagtag ctcttccaaa cctccgagtg
     8041 aattttaacg gcttccttat gagaaccgta ctggaagtta tgggtgggta gcaggtcgta
     8101 gtcgttcatg atttctacca ggtgggctgt tcccttttgg cgcatctggc actgcttatc
     8161 gtccagctcg cgtatttcct tgttgaaccg cctaccgcgt tcctgaattg cctccaaatc
     8221 ggccacatta ttaagatttc cctttacccc gggaatctta catactatag cctttatttt
     8281 cttatcgcga aataccgtgc caattcctcc ccttccggct tgcttaagcc tgatacattt
     8341 ccgctttggg tcgtaaaagg taaagttcag cattccgaaa aggctatgtt ctgcagcaac
     8401 tccggttgac acaatgccta tgttcttctt gtcggcctca tcgttggcaa acatttcgct
     8461 tagcacctct cccaacacat ggctgtctat gggttcggca ggcgcctcaa aaagttcaac
     8521 cttatgggtt atcccatcaa tgtaaattat cacatcacgc tttgctttac cctgaatctc
     8581 aatagcatca aatcccgaaa atttaaggaa tgggccaaag aaacctccca cgttggagtc
     8641 aatcacaata tcggtttggg gtgaaatggt gcatacaatg gattttcccg ttccggagta
     8701 ctgggtaatt ccaccaattg gacccgagga tataacaatt tcgttttcgg gatcattcca
     8761 ctttgtggtt ggcttagtgg catcccaaag caagcgcagg tcgtagcctt taccaccaat
     8821 aaacttttcc ttcacctccg ggggaacagg tttttccatc acctcattgt tgcccacgtt
     8881 tatgtaaagt gttttatcgg tgtaacccct gaaaaggggt ttccactggt aactccagct
     8941 cttcagaagc acatgctgtg cagccatttc ctgaatttcc atactcatcg tttttagtct
     9001 tgcttgctac tactaaaatt cttcaggtaa aaatacaggc aaatttaccg ataaaaaatg
     9061 attattgtca atatgaaaat atttgcatat tgagcaacga tgggactttc aaaagtttta
     9121 caaggtagaa aaccttctgg ttgcaacagc tcaaaatgga ctattcctgt tcaaaagaaa
     9181 ttatggttgt ttgcgaatac tctccattgg gcataagtag tgttgttggg aaatcgggca
     9241 tattaggggt gtttggaaaa tgctggggct caaggcagac tgcccagttt ccactttcag
     9301 ggaatggtat agccgggttg gatacatttg gcctatttat gggtgtatag agctgtaggc
     9361 ccggctgcgt ggtaaagagc ttaaggcaaa ctccattgtc ggggttgcga agcactgcgg
     9421 ccggtttgtc aagcgtgggg ttgttgagcg caaagcaatg atcataaaag ccaccgcagc
     9481 ggtttatgtc ggctccaatg gatttagtcg tcctaaaatc aaagggggtg tgcgaaacat
     9541 tgcgaagctc accggtaggg atgaggcact cgtcaacggg taagtaatgg tcggcactca
     9601 gatggagtaa atggtcaaga acgttctttc taaagccact taggttaaaa tacccatggg
     9661 ttgtcaggtt cacatgcgtg ggcttatcgg tagctgcctt atagcttatg gcaatttgat
     9721 tatcctcggt gatgctgtag gttagccaaa catcgagatt gccgggaaac ccttcatctc
     9781 cgtcggggct gtgcaggaat agctgaatgg tgcaaacgcc gtccctggcc tctactatct
     9841 caggcaccca tatcttccgg tcgaatccgt taattccgcc atgaagctga ttggaaccct
     9901 cgttgcacga aagctggtgg ttgtgcccat ctattgtaaa ttcactacct gcaatcctgt
     9961 tggcgtaccg tccaataatt ccgcccggat ggtagtcggc ctccctgtac gattcaatac
    10021 tgtcgaaccc gtaagccacc tctagctcct tgttgtcctc gcttattagc gctatggact
    10081 taatgagccc gccatagttc gaaatggtac acttcatgcc attgcgcgta gtagcagtgt
    10141 ataaatacac ctccttccca tgaatattgc cccatggaca aatgcgtaaa ttcataataa
    10201 aaagtgatta ggtttgaaag tttaagctct aaagatcttc ctcgtcaacg ataaaaattt
    10261 cctcgttggg ctttttcatc aggtactgct cgcgggcaaa tttttcaagg ttttcgttgt
    10321 tggtttgcag ctcgtttatt tttctcctgt cgtcttgaat tttctgaatg tagtaatccc
    10381 tttcattttc caacctttga atattccgct ttgtctccca catcgaaatc cagttgctgc
    10441 tgtcgaaaat cagcatccat aatagaaaaa taacagcggt aagaaaaaac ttatttttaa
    10501 gttttgacaa aaaccatcgc cagaactttc tgctttctac cgacatagct taacctccgc
    10561 ttatcaaatg tatcacctga acattatcgc cgttggtaat agttgcggtg gagtattgtt
    10621 ccttccgcac cagctcaccg ttaatgcgca ccaccagcat tttaaagctg aattttttaa
    10681 agcgaagcag ctcatcaaca gtaatgttaa ccttggtagt gtcaatatgc tcgggtatcc
    10741 cgtttaaaat tatatccatt tcctttgttt gggcgtgccc ccttcggggt cgggctgctt
    10801 cgggcttcgg caatcctcat tttggccagg gccaaaatga ggcagtgctg ctacgcaccc
    10861 ttcgcatccc tcacgcaaaa ttagtaaact ttacatgcaa ataagcaatt tcgtttcctg
    10921 ttaggtaaac tatggctgcg gttcaccccc ccagaatcgt ccgagcagaa tgcttaccac
    10981 cacgttagcc tgcatggatg cacaaatgct gacccgcgga cacagcgggg gaagatcgtc
    11041 cgaaacctcc gataccccat caccgcacag gtaaatattt ccgtattgaa ccacccgaat
    11101 atcgttcgac tttccccatc ccgcaattcc actgcccgcc actatgggca catcaggcct
    11161 gttaagctga aaaccttcaa tcagcacttt cttcatccgg gcatcgtcaa atgcctcaac
    11221 cagcacatca acaccatcaa acacgccaag cgcagagtcc tcgtccacct ttaattcatg
    11281 cccgcgaaca tctaccatcg gattaatggc aagcaggttt tccctcaagg cgcggatctt
    11341 gctccagcca acctggtgcc aaaaaaagta ctgtcggctt aggttgtctt ctgttaccgt
    11401 gtcgtagtcg gcaataacca aatgaccaac cccaacgcgg gtgagcagca tggccacgtt
    11461 gcttcccaat ccgcccacac cggctattcc aacagaatag cctcttaatg cttcttttac
    11521 ctctctggca gatggcatta gatttttact ttgagcaaaa ttacgtcatt ttagatttca
    11581 ttcacatcgg gttggtttaa tttatctggt gctgaacagt tggcggtatg tgcagttg
//
LOCUS       DAOPHX010000082        11618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_6643_length_11618_cov_6.016345, whole genome shotgun sequence.
ACCESSION   DAOPHX010000082 DAOPHX010000000
VERSION     DAOPHX010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 11618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11618
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6643_length_11618_cov_6.016345"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2031
                     /gene="pnp"
                     /locus_tag="PK502_07165"
     CDS             <1..2031
                     /gene="pnp"
                     /locus_tag="PK502_07165"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPH00632.1"
                     /translation="LTMGKTMLLATVVSAQEAKEDADFMPLSVDYKEKFAASGRFPGG
                     FFKREAKPSDYEVLIARLVDRALRPLFPDDYHAETFVTINLISAEKEIMPDALAGLAA
                     SAALAVSDVPFHGPISEVRVARVNGKLLINPSFQQLEEADIDMIVGATYDNILMVEGE
                     LKEVSEDEMLEAIKFAHEAIKPQCLAQMELAKEIGVVKRTYCHEVNDEQLKASVKEFC
                     YDKVYSIAKSMLPKQERTAAFEAIKQSFMETIPEEERESKEVMVNRYYHAVEKEAMRN
                     MILNEGVRLDGRKTNEIRPIWCEVDYLPMAHGSAIFTRGETQSLTTVTLGTKLDEKII
                     DEVLLQGTEKFTLHYNFPPFSTGEAKASRGVSRREIGHGNLAHRALKNMIPQGNENPY
                     AIRVVSDILESNGSSSMATVCAGTLALMDAGIQIKKPVSGIAMGLITEQGSERYAILS
                     DILGDEDHLGDMDFKVAGTRDGITATQMDIKVEGLSYEVLSKALKQAHEGRMHIMNIM
                     LNTIPAPRADFKPHAPRIEQFEIPRDLIGAVIGPGGKVIQEIQRETSTTINIEEANNH
                     GIVSVFAENAENRDAAVKWIKSIVAVPEVGEVYRGKVKSIMPFGAFVEILPGKDGLLH
                     ISEIEWKRLEKVEDVLKEGDEIDVKLIDVDKKTGKLKLSRKVLLEKPATPKK"
     assembly_gap    2129..2228
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2229..2527)
                     /locus_tag="PK502_07170"
     CDS             complement(<2229..2527)
                     /locus_tag="PK502_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00633.1"
                     /translation="MKTSILFYPSTASLKNKYGGAVLLADYPVKQNLNSLKTISWGWG
                     HYQCELLIHLFFESANKLRKARMPWLYTLFGTVSTLRGKIGLQEVKFVKKTCQKFA"
     gene            complement(2478..3164)
                     /locus_tag="PK502_07175"
     CDS             complement(2478..3164)
                     /locus_tag="PK502_07175"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HPH00634.1"
                     /translation="MANRIMDPIERLMGLRYKSHPWHGVDVGPEVPDVVTAFIEMVTT
                     DTVKYEVDKMSGYLRIDRPQKYSSVLPALYGFIPQTLSDESVAQIARGKTDRKDLSGD
                     GDPLDICVLTEKNIVHGNLLVHAIPIGGFRMIDGNQADDKIIAVLKDDAVYGHIQSID
                     QCPEAVITRLRHYFLTYKDLPGTVRDCEITHVYGVEECHQVINASLDDYRKKFENLDI
                     VLSQYGITKK"
     gene            complement(3472..5637)
                     /locus_tag="PK502_07180"
     CDS             complement(3472..5637)
                     /locus_tag="PK502_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPH00635.1"
                     /translation="MTKKSLIFFLYFWVTLIPVKADEGMWLPLLIGKNTSNMQALGCK
                     LTSEEIYSVNHSSLKDAVVQFGGGCTAEIISPDGLIITNHHCGFSSIQSHSSVQNDFL
                     TNGFWAMSRSEELPNPGLSVRFLVRMEDVTGSVLQGVSPNTPEAERSRIVSEHIKSIE
                     SAAMAAEPGYLAQVKPFYYGNDYYLLIYEVFNDVRLVGAPPEAIGRFGGDTDNWVWPR
                     HTGDFSLFRIYADKNNKPAPYSPDNVPYKPKKFLKISTKGVNEGDFTMIYGFPGRTQQ
                     FITSDAVSLLVNQSNPHKIHLRDIRLSIFNAYMSGNDTIRIQYASKHAGVANAWKKWI
                     GETVGLQRLNAVQWKLEQEQMFTAWANSNPELSREYAGLLPKFKRLYGELAPLSLVAD
                     YRNEAVYAVELLGFASRFKDVLAEFSDSTLDTVKVASLKNRLSSYASTFYKNFSSRVD
                     MEVFAAMMYQYHQQIGGDYQPQFLTQVNNQQQDSWMQLAYNIYSKSIFTDSLMVLNLI
                     NDMDRSNAQIIASDSIYKIYAQFENIYQSKVDTRYREIQDSIDILYRTYVKGLREMQP
                     DKMFFPDANSTLRVAYGKVAGFSPRDGVNYLYYTTIDGIAQKSLQSDVPDYKPPVKLM
                     ELYRNKDFGRYAVNGTVPVAFIATNHTTGGNSGSPVLDSNGNLVGVNFDRCWESTMSD
                     VMFDPNYCRNIALDIRYVLFIIDKFAGAQHLISEMQLDG"
     gene            complement(5642..6244)
                     /locus_tag="PK502_07185"
     CDS             complement(5642..6244)
                     /locus_tag="PK502_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HPH00636.1"
                     /translation="MLLQELLTGYKLVLASGSPRRKDLLGGLNIPFEVWPTEHQDESF
                     PSGLDTEIIPQYLAKKKSLAFTGKLDNSTILITSDTIVACNGMVLGKPADMVEASNML
                     KLLSGNMHTVVTGVCISSSSRSATFQSSTNVYFRNLSAEEIDYYVQHYNPLDKAGAYG
                     VQEWIGFVGVERIEGSYFNVMGLPVQRLYIELVSFLQNNS"
     gene            complement(6254..7210)
                     /locus_tag="PK502_07190"
     CDS             complement(6254..7210)
                     /locus_tag="PK502_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HPH00637.1"
                     /translation="MKFFQLIRYKNLLIIIATMILMRYFIIEPILLSYGLALQMPTGV
                     FIMLVMATVLIAAGGYVINDYFDTRTDLVNKPGEVIVGQEVTRREAIAMHMVLSTLGI
                     LLGLVVSFKVGRPVFSLLFFLTAGILWFYSTLYKRQLIVGNLVVSVLTGLIPLIPLVF
                     ELPLLGNFWEVIAVYQLDLHQLVYWVGGYATMAFLLTFVREIIKDIEDFEGDFTYGRN
                     TIPVRYGIKTAKVTAASMLGITMLALVYLFAAYMRQLSTGEFDFVTFSYFFLFIVVPC
                     FVLLVMILLAQEKKDYHRASGFCKLIMLFGLLYAFIFRWFIV"
     gene            complement(7304..7831)
                     /locus_tag="PK502_07195"
     CDS             complement(7304..7831)
                     /locus_tag="PK502_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HPH00638.1"
                     /translation="MRKNFKEMLALVKAFVFDVDGVFTDGTIIIHPSGELLRTSNTRD
                     GYAVGVAVGRGFPVAIISGGKSDAVGVRFRGLGVQDIYLGVADKMIALEEFRKKYDLE
                     LANLLYMGDDLPDLEVMKLVGVPTCPHDAAYEVKQVAVYISSFNGGRGCVRDVIEQVL
                     RLNGKWGESSDMYMQ"
     gene            complement(7837..8640)
                     /locus_tag="PK502_07200"
     CDS             complement(7837..8640)
                     /locus_tag="PK502_07200"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HPH00639.1"
                     /translation="MTKADSFNRDVVFIGAGNLATSLSVAFFNAGWRVVQVYSRTYQS
                     AATLANRLNAEPTTTLNSLIPDAQLYVVALPDSVIQTVAEQLPSLKGFLVHTSGSTSI
                     EVLSVADVLGYGVFYPLQTFSKARLVEFKNVTVCIEGNSQATLNALSSIAQAITSSVV
                     ALDSEKRRWLHLAAVFASNFTNHMLAAAYRIAEQNGVDFKIFEPLVRETVSKAFLANP
                     KDFQTGPAVRFDQQTMEQHLSMLGFSDLELQNIYKELSLSIQNFAQSKK"
     gene            9137..9886
                     /locus_tag="PK502_07205"
     CDS             9137..9886
                     /locus_tag="PK502_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPH00640.1"
                     /translation="MKILVCISNVPDTTTKVKFTEGDRKLDTNGIQWVINPWDELSLT
                     RALELKDDGNTGVKSVSVAHVGLSSSEPTIRKALAIGADDAIRVNADSSDAYFVAAQL
                     AEVVRKEQFDLILCGIESSDYNGSSVGGMLAEFLGIPSISAVSNLKIENGQVVITREV
                     DGGKEVVTTPTPLVAVVQKGIAKEPRIAAMRGIMTARTKPIKLYEPVPVEIMTEYVKY
                     ELPKPRAACKFVDAENPKQLIELLQTETKLI"
     gene            9912..10877
                     /locus_tag="PK502_07210"
     CDS             9912..10877
                     /locus_tag="PK502_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPH00641.1"
                     /translation="MSVLVYTENWDGKFKKLSFELVSYATRVAEMLNTSCIALSIGNV
                     DDAELKKLGSYGAQKILSVNSDNLKVFDTQNYTEAIAQVAKNNDAKVVVISNNNVGKA
                     VAPRLSVKLKAALAAGVNGLPLSTNPFTVRKRAFSGGAIAHVVLKTDVKIVTLPQNSV
                     DVTETPVNAEVEKISISLPEPKTVVKDVQKQAGKLLLTDAEIVVSGGRGMKSPDNWGP
                     LVELAELLGGATACSRPVSDEGWRPSEEHTGQTGKIIAPNLYIAVGISGATQHIAGVS
                     GSKYIVAINNDKSAPIFEVAQYGIVGDAQKVLPKLVEAVREIKGK"
     gene            11163..>11618
                     /locus_tag="PK502_07215"
     CDS             11163..>11618
                     /locus_tag="PK502_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00642.1"
                     /translation="MTKQVVFALALLVTLVAFGFTITRIIRFFSLTRKGFPVKNIGKR
                     IGIMLKVAFGQTKIFRRPIIGFLHAMVFWGFCLIIFGSIEMVIDGLFGLEKSLSVLGW
                     FHDFIMAAGDIFAPIIAIAIVVFLIRRVFLHIKRFEGIEMKKISHLDANI"
BASE COUNT         3388 a         2630 c         2574 g         2926 t
ORIGIN      
        1 ctaacaatgg gtaaaaccat gcttctcgct acagttgtaa gcgctcagga agcaaaagaa
       61 gatgcagatt tcatgccgct aagcgtggac tacaaggaaa aatttgcagc cagcggacgt
      121 tttccaggtg gtttctttaa acgtgaggca aaaccttccg attacgaggt gcttattgcc
      181 cgtctggttg accgtgccct tcggccgtta ttccctgacg actaccatgc cgaaaccttc
      241 gttaccataa acctaatttc ggccgaaaag gaaattatgc ccgacgcact cgccggcttg
      301 gcagcatccg ctgctcttgc cgtatcggat gttccatttc atggtcccat atccgaagtt
      361 cgcgtggcca gagtgaacgg caagctacta atcaacccat cctttcaaca gcttgaggag
      421 gcagacatcg acatgattgt aggggcaacc tacgacaaca tcctcatggt tgaaggggag
      481 ctaaaggagg tttcggagga tgaaatgctc gaggcaataa agtttgccca tgaagccatc
      541 aaaccccagt gcttggctca aatggagctg gccaaggaga taggcgtggt taaacgtacg
      601 tattgccacg aggtaaacga tgagcagcta aaagctagtg taaaagagtt ttgctacgat
      661 aaagtttaca gcattgccaa atccatgctg ccaaagcagg agcgaacagc tgcctttgaa
      721 gccatcaagc agtcgttcat ggaaactatt ccggaagagg aacgggaatc gaaggaggta
      781 atggttaacc gctactacca tgcagttgaa aaggaggcca tgcgcaacat gatactcaac
      841 gagggcgttc gccttgatgg tcgaaaaacc aacgaaattc gacccatctg gtgcgaagtt
      901 gactaccttc ccatggctca cgggtcggct atattcaccc gtggtgaaac ccagtcgtta
      961 accacagtta cgttaggaac aaagcttgac gagaagataa tagacgaggt gctgcttcag
     1021 ggtacggaaa aatttactct tcactacaac tttccaccct tttccaccgg tgaggcaaaa
     1081 gcctccagag gggttagccg tcgagaaatc gggcacggaa atcttgccca ccgggcatta
     1141 aaaaacatga ttcctcaagg aaacgaaaat ccttatgcaa taagagttgt aagcgacatt
     1201 ctggagtcca atgggtcatc atccatggca acagtatgtg caggcacgct ggcgctaatg
     1261 gatgcgggca tacagattaa aaaaccggtt tcgggcattg ccatggggct catcaccgag
     1321 caggggtcag agcgttacgc catcctttcg gatattcttg gcgacgagga tcacctgggc
     1381 gatatggact ttaaggttgc cggcacgcgt gatgggataa ccgccaccca aatggatatt
     1441 aaggtggaag gactttccta cgaagtgctt tcaaaagctc ttaagcaggc tcatgaaggc
     1501 cgcatgcaca ttatgaatat catgctaaac accattccgg ctccccgtgc cgacttcaaa
     1561 ccccatgcgc cccgaataga gcaatttgaa attcccaggg acctgattgg tgccgttatt
     1621 ggtcccggag gtaaagtgat tcaggaaata cagcgtgaaa cctccaccac catcaacatt
     1681 gaggaggcta acaaccacgg aattgtaagc gtatttgccg aaaatgccga aaatcgcgat
     1741 gctgcagtta agtggattaa gagcatagtt gcggttcctg aagtaggcga ggtttacagg
     1801 gggaaggtta aatccattat gccttttggg gcgtttgtgg aaatattacc cggaaaagac
     1861 ggacttctac acatctcgga aattgaatgg aagcgcctgg aaaaggtgga ggatgttctg
     1921 aaggaaggcg atgagattga tgttaagctg attgacgttg ataaaaaaac cggcaagctt
     1981 aaactctcac gcaaggtgct gctcgaaaaa cccgccacac ctaaaaagta agtgattcaa
     2041 agtaaatgac tcatagtatt aaaaagcctg ccagcaaact tctggcaggt tttttttacg
     2101 aattttacct cctgcaatcc gattttccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnngc aaacttctgg caggtttttt ttacgaattt tacctcctgc aatccgattt
     2281 tcccccttaa agttgatact gtcccaaaaa gtgtgtaaag ccagggcatc ctggcttttc
     2341 ttaacttgtt agcactttcg aaaaataggt ggatgagtaa ttcacattga taatgacccc
     2401 atccccagga aattgtttta agtgaattca aattttgctt cacagggtaa tcggcaagta
     2461 aaacagcacc tccgtattta ttttttagtg atgccgtact gggatagaac aatatcgagg
     2521 ttttcaaatt tttttcggta gtcgtccagt gatgcgttaa taacctgatg gcactcctcc
     2581 actccgtaaa catgggttat ctcacaatcg cgtactgtgc ctggcaaatc cttgtaggta
     2641 aggaagtaat gccttaatct ggtgataacc gcttcgggac actggtctat actttgtata
     2701 tgcccataaa cggcatcgtc cttaagaact gcaattattt tgtcatcagc ctgattgcca
     2761 tcaatcatcc taaagccacc aattgggatt gcgtgaacca gcaggttacc gtggactatg
     2821 tttttttctg taagaacaca aatatccagc ggatcaccat cgcccgataa atctttgcgg
     2881 tcggttttcc cgcgtgctat ttgggctaca ctttcatccg atagggtttg gggtataaac
     2941 ccatagagcg caggcagcac gctggagtac ttctgaggac gatcaatcct aaggtagccc
     3001 gacatcttgt caacctcgta cttaacggta tcggtagtaa ccatttcgat aaaggcagta
     3061 accacatcag gaacttcagg accaacatca acaccatgcc aggggtgcga tttatacctt
     3121 aaacccatca acctctcaat gggatccata attctatttg ccatacaatg cttttttcag
     3181 gataaagttt taaaaacagc ttttacgctc caacacctca acaccatata catacaattt
     3241 ctcaaaagaa atgctttaaa ccttacatgt agtaaaattc ccataagcta tcttaatatt
     3301 ccttcgtcca gggacaggga cgctttagtg atttggtata attcaggcaa aggcactcat
     3361 cggttagcct gaacccgtta aacgtgtaag ccttttcacc tatgatgaaa ttagattata
     3421 ctctaagata gaacaacttg taattcaaaa aatttctgat agctttagga gctagccatc
     3481 aagctgcatc tcgctaatca ggtgttgtgc tccggcaaat ttatcaataa taaacagcac
     3541 gtagcgaata tccagggcaa tatttcggca gtagttgggg tcgaacatca catcgctcat
     3601 ggtactttcc cagcatcggt caaagttaac ccccacgagg tttccatttg aatcgagcac
     3661 aggacttccg gagtttccac ctgttgtatg gtttgttgca ataaaggcaa caggaactgt
     3721 cccattcacc gcatacctac caaagtcctt attgcgatat agctccatca gcttgacagg
     3781 aggtttgtag tcggggacat ccgactgcag tgatttctgg gcaataccgt caattgtggt
     3841 gtaatacaag taatttaccc cgtcgcgagg cgagaatccg gcaactttcc cataagccac
     3901 cctaagggta gagttggcat cagggaaaaa cattttatcg ggctgcatct ccctgagtcc
     3961 cttaacgtag gttcggtata gaatatctat gctgtcctga atttcacgat agcgagtatc
     4021 aaccttagac tggtatatgt tctcaaactg agcgtatatc ttgtaaatgc tgtcggatgc
     4081 aataatttga gcgttgcttc tatccatgtc atttatcaag ttcagcacca tcaggctgtc
     4141 ggtaaaaatt gacttactgt aaatgttgta agccagctgc atccagctat cctgttgctg
     4201 gttgttgact tgagttaaaa attgtggctg gtaatcgcca ccgatttgct ggtggtattg
     4261 gtacatcatg gctgcaaata cctccatatc tacccgcgag ctaaaatttt tgtaaaatgt
     4321 agaagcatag gaagacagcc tgtttttaag cgatgccacc ttaaccgtgt cgagcgtgct
     4381 gtccgaaaat tcagctaaaa catctttaaa acgtgaagca aatccgagca actcaacagc
     4441 atataccgcc tcattcctgt agtcggctac gagcgaaagg ggtgccaact ccccgtagag
     4501 ccttttaaac ttagggagta agccggcata ctccctggaa agttcaggat ttgaatttgc
     4561 ccatgcggta aacatttgct cctgctcaag cttccactga acagcattca ggcgctgcaa
     4621 cccaacagtt tcgccaatcc atttcttcca tgcattggca acccctgcat gtttgctggc
     4681 atattggatt cggatggtat cgttgccaga catgtaagca ttgaaaattg aaagacgtat
     4741 atccctcagg tgaattttgt ggggattgct ctggttaacc agcaggctta cagcatctga
     4801 tgtaataaac tgttgagttc tacccgggaa accgtaaatc atggtaaaat caccctcatt
     4861 aacacccttg gttgaaattt tcagaaattt cttaggttta taaggaacat tatcgggtga
     4921 gtagggtgcc ggcttgttgt ttttatcggc gtagattcta aacagggaaa aatcacccgt
     4981 atggcggggc catacccagt tatcggtatc gcctccaaaa cggcctatgg cttcgggtgg
     5041 tgcgcctacc aaccttacat cgttaaagac ttcataaatc agaaggtagt agtcgtttcc
     5101 atagtaaaat ggcttgacct gagctaagta tcctggttcg gccgccatag cagcagactc
     5161 gatagacttt atgtgttcag aaacaatcct gctccgttca gcttcaggcg tattgggcga
     5221 aacaccttgc agcaccgaac cggtaacatc ctccattcga accaggaaac ggacggataa
     5281 tccggggttt ggcagctctt cggagcgaga catggcccaa aaaccattcg taagaaaatc
     5341 gttttgtacc gatgagtgcg attgaatgga agaaaatccg caatgatgat tggtaataat
     5401 taatccatcg ggtgaaataa tttcggcagt gcagccaccg ccaaactgca ccacggcatc
     5461 cttaaggctg gagtggttaa cgctgtagat ttcttcggag gttagcttgc accccagggc
     5521 ctgcatgttg gaagtatttt ttccaatcag caggggaagc cacataccct catcggcctt
     5581 aaccggaata agggttaccc aaaaatacag gaaaaaaatt aatgattttt tagtcatagt
     5641 tttagctatt attttgtaaa aaactaacca gctcaatata taggcgctgt acgggcaatc
     5701 ccattacgtt aaagtaggag ccttctattc tttcgacacc tacaaaccca atccactcct
     5761 gtactccata ggctccagcc ttatcaagtg ggttatagtg ctgtacgtag tagtctatct
     5821 cttcggcaga aagatttcga aagtaaacat tcgtggagga ttgaaaagta gccgacctac
     5881 tactggagct aatgcagact ccagtaacaa cggtatgcat gttgcccgaa aggagcttca
     5941 acatgttact agcctccacc atatcagcag gtttacccag caccattccg ttacatgcta
     6001 ctattgtgtc ggaggttatc agtatagttg aattatcgag tttgccagta aaggctaacg
     6061 attttttttt agctaggtac tggggtatta tttcagtgtc caaacctgaa gggaaagact
     6121 catcctgatg ttcggtaggc catacctcaa atgggatatt caatccccca agcaaatctt
     6181 ttcgtctggg agagcccgag gcaagcacta gcttgtatcc tgtcaaaagt tcttgtaaaa
     6241 gcatcaataa aagttaaacg ataaaccaac ggaaaataaa tgcgtagaga aggccaaata
     6301 gcatgataag cttgcaaaag ccacttgccc tgtggtagtc cttcttttcc tgcgccagaa
     6361 gtatcatgac cagcagcaca aagcatggaa caactatgaa taaaaagaag taggaaaaag
     6421 taacaaaatc gaattcgccg gtgctaagct gacgcatgta agcggcaaaa aggtaaacca
     6481 gagcaagcat ggtgattcca agcatggaag ctgctgtaac cttggctgtt ttgataccat
     6541 atcggacagg aatggtgtta cgaccatagg taaaatcccc ttcaaagtcc tcgatgtcct
     6601 ttataatctc acgaacaaat gtgagtagga aagccattgt ggcataacca ccaacccagt
     6661 aaacaagctg gtgcaggtcg agctggtaaa cggcaataac ctcccagaag tttcccaaca
     6721 agggaagctc aaacaccagg ggaatgagag gaattaatcc tgttaaaacc gaaacaacaa
     6781 gattcccgac aatcagctgg cgcttgtaca gagtggagta gaaccatagt attccggcag
     6841 tgaggaaaaa gagaagcgaa aaaacaggac gacccacctt gaatgatact acaagcccca
     6901 gcaaaatgcc gagtgtgctt agcaccatgt gcatggcaat tgcctcccga cgggtaacct
     6961 cctgtccaac aataacttct ccaggcttgt taaccaaatc ggtacgggta tcaaaataat
     7021 catttataac atatcctccg gcagcaataa gcaccgtagc catgacaagc ataataaaaa
     7081 cgccggttgg catctgaagc gccaacccat agcttagcaa aattggttca ataataaagt
     7141 acctcatcag tatcattgtg gcgataatga taagcaggtt cttataccga attagttgaa
     7201 aaaacttcat acgtcaagtt aaaatagctg gtgtaatgta aaagtagcct attttgttgt
     7261 tttattccac ttgattttca gcatacctat aaaagcagta gggttactgc atgtacatgt
     7321 cggaactttc gccccactta ccattgagcc ttagcacctg ctcaataaca tctcgaacgc
     7381 atcccctgcc tccgttaaat gaggagatat aaacagcaac ctgcttaacc tcgtaggcgg
     7441 catcatgggg acaagttgga acgcccacca gcttcattac ctcaagatca ggtaggtcgt
     7501 cgcccatgta aagaaggtta gccagctcca ggtcatactt tttacgaaat tcttcaagag
     7561 caatcatttt gtcggccacc ccaaggtaaa tatcctgaac accaagcccc ctgaaacgaa
     7621 ccccaacggc atcggatttt ccgccggaaa taatagccac aggaaaaccc cttccaaccg
     7681 ctaccccaac agcatagcca tcgcgggtat ttgaagttct cagcagctca cccgaagggt
     7741 gaataattat tgttccatcg gtaaaaaccc catcaacatc gaaaacaaaa gcctttacca
     7801 gggccaacat ctccttaaaa tttttacgca tgggcattac tttttacttt gggcaaaatt
     7861 ctgaatgctc aaagatagct ccttgtaaat attctgcaac tccaagtcag aaaatccaag
     7921 catggagaga tgctgctcca tggtttgctg gtcgaaccgt acagcaggcc ccgtttgaaa
     7981 atcctttgga ttggcaagaa aagcttttga aacagtctcc ctaactagcg gctcaaaaat
     8041 cttaaaatca actccattct gctcggcaat cctgtatgct gcagccagca tgtggttggt
     8101 aaaatttgag gcaaaaacag cagcaagatg caaccacctg cgtttctccg aatcgagagc
     8161 tacaaccgat gatgtaatag cttgggctat actgcttaag gcgtttaggg ttgcctgact
     8221 atttccctca atgcaaaccg ttacattttt aaactcaacc agcctggcct ttgaaaatgt
     8281 ttgcagcgga taaaataccc cgtatcccag aacatcagca accgaaagca cttcaattga
     8341 tgtactccca ctcgtatgta ccaggaaacc tttaagagac ggaagctgtt cggctacggt
     8401 ttgaattacc gagtcgggaa gggcaaccac gtatagctgg gcatcgggaa ttagcgaatt
     8461 gagggtggta gttggctctg cgttgagccg gttggcaagg gtggcagcgc tttggtaggt
     8521 tcgggagtaa acctgaacca cacgccaacc ggcattaaaa aaggcaacag aaagtgaagt
     8581 ggccagattg ccagcaccaa taaaaacaac atccctattg aaggaatcag ccttggtcat
     8641 caccatatat tttaaagcat aaagatagct aataaaataa ggttactgga gaaaggacat
     8701 tcaagaatta aggcaaggga taaagaaaat cgaagcctga gtggcttttg acgaaaactt
     8761 gtctcattca gaggagttta ccctgcgaca ggctcagcgt ccgggcaggt ccggttctta
     8821 actgaacttg cctggctttt agcaaacagg cgttgggatc tgacggattc atggttcata
     8881 tagctcaacc tctttcctta gcttcttcac tctcccggtt cacagctacg tcattggcca
     8941 tcgcttctct acctgctcaa ctccaaaaaa gcgccataac ctactcaact tcagtgaatt
     9001 ttgacgactg tgggtataaa ccccacccaa caaatttttc ccattaataa ctttgattat
     9061 ttttgcaatt ctaatcaaga aaccttcaag tgtaaagtag taaaccaata cattttaaaa
     9121 cttatactat agtctgatga aaattttagt gtgtatcagc aacgtacccg acaccaccac
     9181 caaggtgaag tttacagagg gagaccgcaa gctggatacc aacggtattc agtgggttat
     9241 taacccatgg gatgaacttt cgctcaccag agcgttggag ttgaaggatg atggtaacac
     9301 cggggtgaaa agcgtatccg tagcccacgt aggtttatcc tcatcggaac caaccatcag
     9361 aaaggcgtta gctataggag ccgacgatgc catcagggtg aatgccgact cgtccgatgc
     9421 ctactttgtg gctgctcagc ttgccgaggt ggtaaggaag gaacagtttg accttattct
     9481 atgtggaatt gagtcgagcg attacaatgg atcttcggtt ggaggtatgc ttgccgaatt
     9541 tttaggaata ccctccatat cggcagtttc aaacctcaag atagaaaacg gacaagttgt
     9601 tatcacccgc gaggttgatg gcggaaaaga agtggttacc acccccaccc cattagttgc
     9661 cgttgttcaa aagggaatag ctaaggaacc aagaattgcc gcaatgcgcg gtattatgac
     9721 agcacgcact aagccaataa aactttacga gcctgttcct gtagaaatca tgaccgaata
     9781 tgtcaagtac gagctaccca aaccacgcgc agcttgtaag tttgttgatg ctgaaaatcc
     9841 aaaacagctc attgagctgc ttcaaaccga aaccaagtta atttagcctt cacccattaa
     9901 acgattacga tatgtcagta ctagtatata cagaaaattg ggacggaaaa ttcaaaaagc
     9961 taagctttga gctggtatca tatgcaacaa gggttgccga aatgctgaat accagctgca
    10021 ttgccctttc gattggaaat gttgatgatg ccgagttgaa aaagctgggt agctatggcg
    10081 cccaaaagat attgagcgta aacagtgaca acctgaaggt attcgacaca caaaactaca
    10141 ccgaagctat agcccaggtt gcaaaaaaca acgatgcaaa agtagttgta atttctaaca
    10201 ataatgttgg caaagccgtt gcgcctagat tatccgtaaa gttaaaagct gcgctagcag
    10261 caggtgtaaa tggcctaccg ctatcaacca atccctttac cgttcgtaag agagcttttt
    10321 cgggtggtgc cattgcccac gttgtgttaa aaaccgacgt aaaaatagtt accctacccc
    10381 aaaactctgt tgatgtaacc gaaactccgg ttaacgctga agtggaaaaa attagcatca
    10441 gcctacccga acctaaaacc gtggtaaagg atgtacagaa gcaagcaggc aaactgctgc
    10501 tcactgatgc cgagattgta gtttcgggcg gacgcggaat gaaatcgccc gataactggg
    10561 gaccactggt ggagctggcc gaactgcttg gaggtgctac cgcatgctcg cgtccggttt
    10621 cagatgaagg ttggcgtccc agcgaggagc acaccgggca aaccggaaaa ataatcgccc
    10681 caaaccttta cattgcagtt ggtatctcgg gagcaaccca gcatattgcc ggtgtgagcg
    10741 gttcaaagta tattgtagct attaataacg ataagagtgc tccaatattt gaggtagccc
    10801 agtacggcat tgtaggcgac gcccaaaagg ttcttcccaa actggtagaa gctgttaggg
    10861 aaattaaagg aaaatagcca atgccgatgt ttggcagctc caatattggt gttttggctc
    10921 cacgagcaga aaaaattgct gcctaaaaaa acaacatgca ggagataaac aagcagtgct
    10981 cctgcatgta aggttatgtt taatgaatag attcaggtga ttaaatccac actacgcagc
    11041 aggaggatgt ccggttgcac gaagttccat aaaatagccc atgatatgaa cataggcggc
    11101 atacaccatt gcattacctg caaacccgac gaaacaatag aataataaaa aactgaagct
    11161 ccatgaccaa acaagttgtt tttgcattag cattgctggt aacgctggtt gcttttggtt
    11221 ttaccataac cagaatcatc cggtttttct cgttaacccg taaggggttt ccggttaaaa
    11281 atattggaaa acgtattggg ataatgctca aggtggcatt tggccaaacc aaaatttttc
    11341 gtcggcccat cattgggttt ctacacgcca tggttttctg gggattctgc ttaatcatct
    11401 ttggaagcat cgaaatggtc attgacgggc tgttcgggct tgaaaagtcg ctgagcgttt
    11461 taggttggtt tcacgacttc atcatggctg ccggcgatat ctttgcacct atcatagcca
    11521 ttgccattgt ggttttttta atacgcaggg tattcctgca tattaaacgc ttcgagggca
    11581 tcgagatgaa gaaaatttcg cacttagatg ccaacatc
//
LOCUS       DAOPHX010000083        11508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_6742_length_11508_cov_5.068716, whole genome shotgun sequence.
ACCESSION   DAOPHX010000083 DAOPHX010000000
VERSION     DAOPHX010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11508
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6742_length_11508_cov_5.068716"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..3220
                     /locus_tag="PK502_07220"
     CDS             128..3220
                     /locus_tag="PK502_07220"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HPH00643.1"
                     /translation="MGSNQQANTMSSYDTTQYKKFNNITGWAIFAIALVVYLCTIEPT
                     ASFWDCGEFIASGYKLEVGHPPGAPFFMLLVRFFTMFAPSAQLVPVFANAMSALASAF
                     TILFLFWSITHLARKIIGSESGSFSASQLILIFSAGAVGALTYTFSDTFWFSAVEGEV
                     YATSSLFTAIVFWAILKWEEAADEPYANRWIVLIAYLMGLSIGVHLLNLLAIPAIVLV
                     YYYKNYTYSHKGFFTALLLSAAILLIVMYGIIQGAVAIASGFELFLVNGLGLPLMSGV
                     VFFILTLIVLLVLGLRYTYRRRKYLLNTVLLGFTMILMGYSSYAVIVIRSSANPPMDQ
                     NSPDNMFSLKYYLNREQYGDRPLLYGQSFSAQPVDRTDGRLTYAPENGRYVVVRKQSD
                     YIYDPRFEMPFPRMYSSDPQHISAYKSWTNFKGRPVNVNTQDGKQQTIYTPTFGENLK
                     FFFSYQLGFMYFRYFMWNFSGRQNDIQGHGDVMKGNWICGIPFIDNARLGSQELLPDT
                     YKQNKGRNRYFMLPLILGLSGLIFQYRRRMKDFWVVMTLFVMTGIAIVVYLNQTPYQP
                     RERDYAYAGSFYAFSIWVGLGVMALYELTRRIKDGSATALATGAACTLMAPTLMAFQN
                     WDDHDRSGSYIPADFGYNMLVGCKPNSILFTYGDNDTFPLWYNQEVEGVRTDVRVANL
                     SYLRGDWYIEQMQRKVYESDPLPLRMTRKQYFSGKRDVVLVFDRIKEPINLNMAVNFM
                     LSDDPAAELKSPFDPGERINYIPSSNLFLPVDRQQVIKTQTVLPSYYDRIVDTMRWKL
                     NKNMVMKDGQVILDLLGTNSWVRPIYFGTTVNSDTYHGLNKYFHLEGLMYRILPVEAG
                     EQWGVGSVNTSEMYGNLMGKYRYRGLNNPKVYIDENKFRIVSNYRNLYARLANALVDE
                     GKTDSALVVLNHCMEVIPASTVPLNYYVLPIIEGYYRAGDKTSAIRISRLLMDQSVKE
                     LDFILTQLNDRQQQNLFNSVQLDMAILQDLVRMAQSYEKGNHYEELNRTFNRFLSMLQ
                     "
     gene            3230..3865
                     /locus_tag="PK502_07225"
     CDS             3230..3865
                     /locus_tag="PK502_07225"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPH00644.1"
                     /translation="MNLSPPRFFQRFFKNIIWNFPDEKEGVFLTFDDGPTQGVTPWVL
                     DQLARYNAKATFFCLAKNVEMNPDIFERVVREGHAIGNHSYSHIKGWRTDTTQYIQDV
                     IYANSFLHTNLYRPPYGRITARQFRLITYQYKIVLWDVLSMDYSRRVSPKKVTKFVID
                     NVKPGSIVVFHDSLKAERNLRYALPRVLDAITATGMVCKPIVIPRKNIRES"
     gene            3820..4989
                     /locus_tag="PK502_07230"
     CDS             3820..4989
                     /locus_tag="PK502_07230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPH00645.1"
                     /translation="MQANSYSKEKYPGELKKKGILFIVQLPPPVHGASMMNSMVYSRL
                     LGDLRYRCRLVRLNFARNLNDLQKFRFRKLGRSFTVFLEICWKALTFQPSIIYFSMVP
                     LGTTLLRDSAYLLAVRLLLPKSRKIIHLHRPGLYEFNQKYRLSWFYRFIFKGCEIIHL
                     TESLVEHEITPLQLTNVKVYAVPNSIEVDAAQHDAGVARMRYNILFLSNLFPHKGFIP
                     LVKAHALLKNNFPGLTLTIAGEIPNSDVEGRLRETIRELCLGDSVKVLGKVSGEQKSL
                     LYRQAGIFVLPSEQEYFPLVILEAMAARTPVITSGRHNLESLFTDMHDLLFVDDLSPE
                     GIAKTIQKLLDNPALASSIGENGYHRFCELQVQSQRMMEDLFNGCADYALLPTRP"
     gene            complement(4946..6154)
                     /locus_tag="PK502_07235"
     CDS             complement(4946..6154)
                     /locus_tag="PK502_07235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPH00646.1"
                     /translation="MVRRIKKLPITIFTSETAPAFAGDGLNALLLAESLIKKGYSAQL
                     ICLNPNKLLPKSEVINGVTIRRIAYHYKNKRGRLWLRVNMLLYILGAARKQGYWLIYG
                     AMPANRLLVLTGWILGKKVFFRSTLYSFDDASTLISSKKNPVKAIYRFIYGRVAGYHA
                     LNSVFAEAWRKTYNEIIPIFVSPQGTTIPYPAPNIQNRKNARSELGLPPDPIIVLMVG
                     HLITRKGFPDIFEWLAKANCSFLLLHVGTIHAPESNIISIRNNEMRQNIVAGQNLLGE
                     RVKFMECSQRPSLYYYAADILLLASAAEGYPPNTLNEAMAAGLPILSNRIAGSTDYIS
                     DNHNGFLYTGFDEFISRFSQLVSNQDIRQQLGNNARLYAEKYLDVNPIAKNLLKFMDE
                     SAARHSRHSH"
     gene            complement(6418..9477)
                     /locus_tag="PK502_07240"
     CDS             complement(6418..9477)
                     /locus_tag="PK502_07240"
                     /inference="COORDINATES: protein motif:HMM:NF016345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic secretory protein-like protein"
                     /protein_id="HPH00647.1"
                     /translation="MKRIVLRAVGIALLPLIWGSSNFVNAQYFGRNKPIYKNFKFKVY
                     QTPNFEIYHYLNNDSLLNHMAMSAEKWYDRHYQVFRDSIVKRNPILIYDNHADFQQTT
                     AISSTVGVGTGGVTEALKNRVIFPLTETWGQTNHVLGHEMVHAFQYNSLIHGDSTSLN
                     SVRNLPLWMVEGMAEYLSLGTYDYQTAMWMRDAVLNDNFPTLEDMSFKPNEYFPYRWG
                     HAFWSFIGKTFGDSLINPIFKETAKRGYDMALRRYIGLTEKSFSTLWKSVYYDYYKRI
                     LPDSSDNPSGNRILFERNAGEVNISPAVSPDGRYVAFYSEKDLFSIDLFVAHAISGKI
                     IRRLSRIVHNNEIDALNFIESAGTWSPDSRQFAFVAFSKGRNKLLVIDVYDDKLTSEM
                     YIEGVPSFNYPTWSPDGKSIVVSGLVNGVNDLYSMDIQTGKVTRLTNDPWCNIHPSWS
                     PDGSAIVYVTDKPSEIQAANKSQQVGYNLATLDVRTNNIKVYEVFPGADNLNPMFSGN
                     GHTIYFLSNSDGYRNLFKYDLNTGETYRLTRILTGISGMTPFAPAMSVAHENDEICYS
                     HFYNSKYSIYTADTTDFNPVKVDPYHVDFSASILPPLNRATEGLVDQNLTSNDLPEHL
                     VDSFKTVKFRPQFKLDYISNIGMGISTSPYGTGMAGGVEMLFSDITGAKMLYTGLSLN
                     GEVYDFGGQVSYINQRKYITWGVTLSHIPYSFGAYGYDTLHRVDNGNPIIIEDFQVLQ
                     YRMFETAGGGMAYFPLSQTQRFELTASVARYYYRVDIYSNYYVDGFYIGADRHKGDAP
                     PGFWMQQTNLAYVYDNSDFGIASPMRGNRVRLQVDKTLGDYNYWGSLVDLRKYIFAKP
                     FSFAFRGMYYGRYGTSIENSVLYPLYIGYPWYVRGYDSNVFIDYGQSGTTSIDQLTGD
                     QMVVTNFEVRVPFTGPERLTLIKSGAFYTELALFADAGIAWSKGYPPSLKWKPTSLND
                     RTPFVSAGLSIRINLFGMMVLEPYYAIPFQLGGFKSANWGINFLPGW"
     gene            9742..10023
                     /locus_tag="PK502_07245"
     CDS             9742..10023
                     /locus_tag="PK502_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00648.1"
                     /translation="MSKTINLNVMDEKLTILGIKIEDRMKEAGEVQKLLSRYGCSIKT
                     RLGLHEVAGDYCSTSGLIILELAGPAEDQRKLHDELQKIQGVLVRKMEF"
     gene            10188..11021
                     /gene="surE"
                     /locus_tag="PK502_07250"
     CDS             10188..11021
                     /gene="surE"
                     /locus_tag="PK502_07250"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPH00649.1"
                     /translation="MQVEKGQDTNRVILVTNDDGYKSSGMEALIEMVKPLGRVVVVAP
                     EEGNSGMSHAITIKVPLRMKHRNRDDNVELYTVNGTPVDCVKLAMNELFAKIPPDLMV
                     SGINHGSNASISVVYSGTIAAAIEGCLYGIPSVGFSILDYGEYPDFRASILYGREILK
                     NVMEKGISPECCLNVNIPVLPPQEIKGVKVARQNRGSWREEFEKRTDPRGVNYYWLTG
                     YFHNDEPEATDTDEYFLSKGYITVVPVNVDFTDYREMKKLQGWENDLYNAVKNELSQA
                     K"
     gene            10999..11295
                     /locus_tag="PK502_07255"
     CDS             10999..11295
                     /locus_tag="PK502_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00650.1"
                     /translation="MNSAKLNRQRVGYLFGILLPLLVLVVIFFTMYTGKSFWAFLQIM
                     WIQGNLSQVLSLAVIPNLAIFFIFIWTKKMKAAQGVLGATFILAFLIIILRFAR"
     gene            11306..>11508
                     /locus_tag="PK502_07260"
     CDS             11306..>11508
                     /locus_tag="PK502_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPH00651.1"
                     /translation="MRYFIVAGEASGDLHASNLMRGIKAKDSSAEFRFFGGDLMKAQG
                     GTLVKHYRDMAFMGFLEVLLNLRT"
BASE COUNT         2944 a         2504 c         2842 g         3218 t
ORIGIN      
        1 tgtgcgtaac aatggtaata aaatatccgc caggttggga ataatcgtat cctggtaatc
       61 gaatagattc atgaccatta gttggctgta tgtttttatt ggttaatcaa tgccaaaaat
      121 tttacctttg gggtcaaatc aacaggcaaa caccatgagc agctacgaca ctacccaata
      181 caaaaaattc aacaatatta caggctgggc aatttttgcc atagcgcttg tggtttacct
      241 ctgtaccata gagcccacag ccagtttctg ggattgcggg gagttcatag ccagcggtta
      301 caagctggag gtaggacacc cccccggtgc acccttcttc atgcttttag tgcgcttttt
      361 taccatgttt gcaccttcgg cacagctggt tcctgtattt gccaacgcca tgtcggcgct
      421 agcaagcgct tttaccattc tattcctatt ttggagcatt acccatttgg caagaaaaat
      481 tattggttcc gaaagtggaa gttttagtgc cagccagctg attcttattt tttcggcagg
      541 agcagtagga gcgctgacct ataccttttc cgataccttt tggttttcgg ctgttgaggg
      601 ggaggtttac gccacctcat cgttgtttac cgccattgta ttttgggcta ttctaaagtg
      661 ggaggaggct gccgatgagc cctacgccaa ccgatggatt gttctgatag cctatcttat
      721 gggtttgtct attggcgtgc atctccttaa cctgctcgca attcctgcaa ttgtgctggt
      781 ttactactac aagaactaca cctactcgca taaaggtttt tttacagcgc tgctgctatc
      841 ggcggctatt ctgctcattg taatgtatgg catcattcag ggggcggtgg ccatcgcttc
      901 aggatttgaa cttttccttg ttaatgggct gggactaccc ctgatgtcgg gagttgtttt
      961 cttcattcta accttgattg ttctgctggt gcttgggcta cgctacacct accgccgaag
     1021 gaagtacctg ctgaatactg ttttgctagg tttcaccatg attcttatgg gttacagctc
     1081 ttatgcggtt attgtaatcc ggtcatcggc taaccctcca atggaccaga atagtccgga
     1141 taacatgttc tcgctaaagt actacctcaa ccgcgaacag tacggcgata ggccgcttct
     1201 atacggtcaa tcgttcagcg cccaaccggt tgatcgcacc gatgggaggt taacctatgc
     1261 ccccgagaat gggcggtacg ttgttgtcag gaagcaatcc gactacattt acgatccccg
     1321 ttttgagatg cctttcccgc gcatgtacag cagcgatccg cagcacatca gcgcctataa
     1381 aagttggacc aactttaagg gtaggccggt taatgttaac acccaggatg gtaagcagca
     1441 aaccatctat actcctactt ttggcgagaa tctgaaattt ttcttcagct accagctggg
     1501 tttcatgtac ttccggtact tcatgtggaa cttttccgga cggcaaaatg acattcaggg
     1561 ccatggcgac gtgatgaagg gcaactggat ttgcgggatt ccttttattg ataacgccag
     1621 acttggttcg caggagttac tccccgacac ctacaagcag aacaagggac ggaacaggta
     1681 tttcatgctt cctctcattc tcggattatc agggttgata ttccagtaca ggcgtaggat
     1741 gaaggatttc tgggtggtga tgacgttgtt tgtaatgacc ggcatagcta ttgtggttta
     1801 cctaaaccaa accccatacc agcctcgaga aagagattat gcctatgctg gtagctttta
     1861 cgctttcagc atttgggttg ggttaggtgt gatggcccta tacgagctta ctcgtagaat
     1921 taaggatggt tcagccaccg ctttagccac gggcgctgct tgcaccctga tggcgcctac
     1981 cctgatggcc ttccagaact gggacgatca cgaccgatcg ggaagctata ttcctgccga
     2041 ttttggatac aacatgctgg ttggctgcaa gccaaactcc atcctgttta cctatggcga
     2101 taacgatacc tttcccttgt ggtataacca ggaggtggag ggcgtacgaa ccgatgtgag
     2161 ggttgccaac ctcagctacc ttcggggtga ctggtatatt gagcagatgc agcgcaaagt
     2221 ttatgagagc gatccccttc cgctgagaat gaccaggaaa cagtacttct ccgggaaacg
     2281 agatgtggtg ctggttttcg acagaattaa ggagccaatc aacctgaaca tggcggtaaa
     2341 cttcatgctt agcgacgatc ccgctgctga gctgaaatcg ccattcgatc cgggcgagag
     2401 gattaactat attccctcca gcaacctgtt tttgccggtt gacaggcagc aggttataaa
     2461 aacccaaacc gttttgccct catactacga cagaattgtt gataccatgc ggtggaagct
     2521 gaataaaaat atggtgatga aggacgggca ggtaattctc gacctgctgg gaactaatag
     2581 ctgggtgcgc ccaatttact ttggaactac ggtgaatagc gatacatacc acgggcttaa
     2641 taagtatttc caccttgaag gattgatgta caggatttta cctgttgagg caggagaaca
     2701 gtggggtgtg ggcagcgtaa acacctccga aatgtacggc aacctgatgg gtaagtacag
     2761 gtatagaggg ctgaacaacc cgaaagttta cattgatgag aacaaattcc gaattgtctc
     2821 caactatagg aatctttatg cccgcctggc caacgcattg gtggatgagg gcaaaaccga
     2881 ctcggctttg gttgtgctaa atcattgcat ggaggttatt ccggcaagca cggtgccgtt
     2941 gaactactac gtgctgccaa ttatcgaggg ttattacagg gcaggagata aaacttcggc
     3001 cattagaata tccaggttgc taatggacca atcggtaaaa gagcttgatt tcatacttac
     3061 ccagcttaat gataggcagc aacagaacct cttcaacagt gtgcagctgg atatggccat
     3121 actgcaggac ttggttcgca tggcccagag ttacgagaag ggtaatcatt acgaagagct
     3181 gaatagaaca tttaaccgtt ttctgtcgat gttacagtaa atcaacatga ttaaccttag
     3241 tcccccccgt ttctttcagc gtttttttaa aaatattatt tggaattttc ctgatgaaaa
     3301 ggagggggtc tttttaacat ttgatgatgg gcctactcaa ggggttaccc cctgggtact
     3361 cgaccagctt gcccgctaca acgctaaggc tacatttttt tgtctggcta aaaacgtgga
     3421 gatgaatccc gatatctttg aacgagttgt tcgtgagggg catgctattg ggaatcactc
     3481 ctatagccat attaagggtt ggagaaccga tacaacccag tacattcagg atgtcattta
     3541 cgccaacagc ttcctgcata ccaacctcta ccggccgcct tacggcagaa ttactgcacg
     3601 gcagtttaga cttatcacct atcagtacaa aatagtgctt tgggatgtgc tcagcatgga
     3661 ctacagccgg agggtttcgc caaaaaaggt tacaaaattt gtcatagaca atgtgaagcc
     3721 aggttcaata gtggtttttc acgattctct taaggctgaa aggaatctga ggtatgcctt
     3781 gccacgggtt ttggatgcca ttactgcaac cgggatggta tgcaagccaa tagttattcc
     3841 aaggaaaaat atccgggaga gttgaaaaaa aagggaatac tattcatcgt tcagctgcca
     3901 ccacctgttc acggagcaag catgatgaac agcatggtgt acagcaggtt gttgggtgac
     3961 ctaaggtatc gctgtaggtt ggtcaggtta aattttgcta gaaatctgaa cgatctgcag
     4021 aagtttcggt ttcggaagtt aggtcgttct tttactgttt ttctggagat atgctggaag
     4081 gcattaacct ttcaaccttc cattatatac ttctcgatgg ttccgctcgg aactacactt
     4141 cttcgcgata gcgcatactt gctggctgtt aggcttttac ttcctaagtc gagaaaaatt
     4201 attcaccttc accgtcccgg actctacgag tttaatcaga aatacaggct ttcatggttc
     4261 tataggttta tatttaaagg atgtgaaata atacacctta ccgaatcact ggtagaacat
     4321 gagattacgc cacttcaact aacaaatgta aaggtgtatg ctgtgcccaa ctccattgag
     4381 gttgatgcag cacagcatga tgccggcgtt gccaggatga ggtataatat cctgtttctc
     4441 tcaaatcttt ttcctcataa gggttttatt ccgctggtga aggcccatgc cttgttgaag
     4501 aacaattttc cggggctgac gcttactatt gccggagaga ttcccaattc cgacgttgag
     4561 ggtagattgc gggaaacaat ccgtgagctt tgccttggcg atagcgtgaa agttctggga
     4621 aaggtttccg gtgagcaaaa atcattactt taccgtcagg caggcatttt tgtgctgccc
     4681 tccgaacagg agtatttccc gctggtaatt cttgaggcta tggctgcccg aaccccagtg
     4741 attacaagtg gtaggcataa cctggaatcg ctgtttaccg atatgcacga cctgctgttt
     4801 gttgatgatc tttcacccga aggcattgct aaaacaattc aaaagctgtt ggacaaccca
     4861 gcacttgcca gtagtattgg cgaaaatggt taccaccgtt tttgtgagct ccaagtgcag
     4921 agccaaagaa tgatggaaga tttattcaat ggctgtgccg actatgcctt gctgccgact
     4981 cgtccatgaa tttaagtagg tttttggcta tgggattgac atcaagatac ttttcggcat
     5041 acagccgggc gttattgccg agctgctggc gaatatcctg attggagacc agctggctga
     5101 acctggaaat gaattcatca aaccccgtgt acagaaaacc attgtggtta tcggaaatgt
     5161 agtcggttga gcctgcaatc ctgtttgaga gaattggcaa tcctgctgcc atggcttcgt
     5221 ttaacgtatt tggggggtag ccttctgcag ccgaggctag aagtaatata tccgcagcat
     5281 agtagtagag tgaggggcgc tggctgcatt ccataaactt aaccctttca cccagcaggt
     5341 tttgccctgc tactatgttc tgcctcattt cgttgttgcg gattgagata atgttcgact
     5401 ccggggcatg aatggttccc acgtgtagga gcagaaagct acaattcgcc tttgccaacc
     5461 attcgaatat gtcgggaaaa ccctttcggg ttatcaggtg tccgaccatt agtacaatta
     5521 tcggatcagg gggtaagccc agctcactcc gggcattctt tctgttttga atgtttggtg
     5581 caggatatgg tatggtggtt ccctggggcg atacaaaaat cggaatgatt tcgttgtagg
     5641 tttttctcca tgcctctgca aagacgctgt ttagcgcatg gtatcctgct accctaccat
     5701 aaatgaacct gtagatggct ttgacaggat tttttttgct cgaaattagc gtggaggcat
     5761 cgtcaaaaga gtagagggtt gaccggaaaa aaaccttttt cccaagtatc caaccggtga
     5821 gtactagcag acggtttgcc ggcattgctc cgtatataag ccagtagcct tgctttctgg
     5881 cagcaccaag aatgtaaaga agcatgttca ctcttagcca taatctacct cttttgtttt
     5941 tgtaatggta ggctattctt ctaatggtaa ccccattgat aacctctgat ttaggtagaa
     6001 gcttgttagg gtttaggcag attagctgcg cactgtaccc tttttttatc agggattcag
     6061 ccagaagtag ggcattcagc ccatcaccgg caaatgcggg tgcagtttcg gaggtaaaga
     6121 tggtaatggg caacttcttt attcttcgca ccattgctag aggctggtgt taaggtagat
     6181 tttttggttt gaaaaaagtg taggcaataa aagtggatac cttacttgtt gaatgcgtta
     6241 aatatttatt ctaatacaac aattttttca tactatcagt atcattcgtg tcctatcgaa
     6301 tttaatggta cacggaaaac acagattaaa ctgatttgaa ctgattaatc tgtgattttc
     6361 cgtcgcatcc gcgtcatccg agtcctactt ttttatttat gagaaaaaaa gaatcggcta
     6421 ccatcccggc aggaagttga taccccagtt ggccgatttg aatcccccca gctgaaatgg
     6481 aatggcgtag tagggctcaa gcaccatcat cccaaacagg tttattcgta tgcttaggcc
     6541 ggcgctaaca aagggagtgc ggtcgttgag ggaggtaggc ttccacttta ggctgggtgg
     6601 gtaccctttg ctccacgcta tgccggcatc ggcaaatagg gcaagttcgg tataaaatgc
     6661 cccggattta ataagggtta acctttcggg accggtaaac ggcactctta cctcgaaatt
     6721 tgtaaccacc atttggtcgc cggttagctg gtcaatgctg gtggttcccg actgcccgta
     6781 gtcaataaaa acattgctgt cgtaacccct cacgtaccat gggtagccaa tgtaaagcgg
     6841 gtatagaacg ctgttttcaa tagatgttcc gtatcttccg tagtacatcc ctctgaatgc
     6901 aaagctgaag ggtttggcaa atatgtattt cctcaggtca accaggctac cccaatagtt
     6961 gtaatcgccc agggttttgt caacctgaag cctgacacgg tttcccctca tgggcgaggc
     7021 tatgccaaaa tcggagttgt cgtaaacgta agccaggttg gtttgttgca tccagaaacc
     7081 cggaggagca tcgcccttgt gcctgtcggc tcctatgtag aagccatcaa catagtagtt
     7141 tgagtagatg tcaaccctgt agtagtaacg tgctaccgaa gcagtgagtt caaatctttg
     7201 agtttgcgaa agcgggaagt aggccatacc tcctcctgcc gtttcaaaca tgcggtactg
     7261 taacacctga aaatcctcta tgattatcgg gttaccatta tcaactcgat gcagggtgtc
     7321 gtagccataa gctccaaagg agtaggggat atgcgaaagg gtaacacccc aggttatgta
     7381 ttttctttgg ttgatgtatg acacctgtcc accgaagtca taaacctcgc catttaacga
     7441 taggccggtg tagagcatct tggcgccggt gatatcgctg aagagcattt ccacgccacc
     7501 cgccattcct gttccatagg gcgaagtgct aatgcccatt ccaatgttgg agatgtagtc
     7561 gagcttgaac tggggtctaa actttaccgt tttaaatgag tcaaccaggt gttcaggaag
     7621 atcattgctt gttaggtttt ggtcaacaag cccttcggtg gcccggttaa gtggtggtag
     7681 aattgaggcg ctgaagtcaa catggtacgg atcaaccttc accgggttaa agtcggtggt
     7741 atcggcggtg tagatggagt acttgctgtt gtaaaaatgg gagtagcaga tttcatcgtt
     7801 ttcgtgggcc acgctcatgg caggtgcaaa gggggtcatc cccgatattc cagttaggat
     7861 tctcgtcagg cggtaggttt cgcccgtatt gaggtcatac ttgaagaggt tgcggtagcc
     7921 gtcgctgttg gagaggaagt agatggtgtg gccgttaccc gagaacatgg gatttaggtt
     7981 gtcggctcct ggaaatacct cgtaaacctt aatgttgtta gttctcacat caagtgttgc
     8041 caggttgtag cccacctgct ggcttttgtt ggctgcctga atttctgagg gtttatcggt
     8101 aacataaaca atggctgagc catcgggcga ccacgatggg tgtatgttgc accaagggtc
     8161 gttggtcaac cgggttacct ttccggtttg aatatccatg ctgtagaggt cgttaacgcc
     8221 gtttaccagc cccgagacca ctatagactt tccgtctgga gaccaagttg ggtagttgaa
     8281 cgatgggaca ccttcgatgt acatctcgct ggtgagctta tcgtcgtaaa cgtcaatgac
     8341 cagcaacttg tttctgccct tgctaaaggc cacaaaggca aactgccggc tatcgggcga
     8401 ccatgttcct gccgattcaa tgaagttgag ggcatcaatc tcgttgttgt ggactatgcg
     8461 cgacaggcgg cggattattt ttccgcttat ggcatgggct acgaataggt caatcgaaaa
     8521 caaatctttt tcggagtaaa aagctacata tcggccatcg gggctaacag ctggcgaaat
     8581 gttgacttct ccggcattcc tttcgaatag aatgcggttg cccgacgggt tgtcggagct
     8641 gtcggggaga attctcttgt agtagtcgta gtaaaccgat ttccacaggg tagagaagga
     8701 tttttcggtg agcccgatgt accttcgaag ggccatatca tagccccttt tggcagtttc
     8761 cttgaaaatc gggttgatga ggctgtcgcc aaaggttttt ccaatgaacg accaaaacgc
     8821 atgaccccaa cggtatggaa aatactcgtt gggtttgaac gacatatcct caagggtggg
     8881 aaagttgtcg ttgagcactg catcgcgcat ccacatggct gtttggtagt cataggttcc
     8941 cagcgaaagg tattcggcca tgccttcaac catccatagc gggagattac gcaccgagtt
     9001 tagcgaggtt gaatcaccgt gtatgaggga gttatactga aaagcatgaa ccatttcgtg
     9061 gccaagtacg tggttggttt gcccccaagt ttcggtgagg ggaaaaatca cccggttttt
     9121 aagggcctcg gttaccccgc cggtaccaac accaactgta ctcgaaatgg ctgtagtttg
     9181 ctgaaaatca gcatggttgt cgtagattaa aatgggattt cgttttacga tggaatccct
     9241 gaaaacctgg tagtgcctat cataccattt ctcagctgac atggccatgt ggttgagcag
     9301 gctatcgttg ttaaggtaat ggtatatttc aaagttgggc gtttggtaaa ccttaaactt
     9361 gaaattcttg tatattggct tgttccggcc aaaatactga gcattaacaa agttcgatga
     9421 tccccagatt agaggcaaga gagcaattcc aacagctcgt agtactatcc ttttcatgcg
     9481 attatccatt tttctgtccg catcatagct tgtaaccagt atcgcaaaaa aatgttgttt
     9541 tcaacaattt gaaatacagt atcccaaacc ggttaaaaat taaacctaca gtagccaaat
     9601 ataagaacct gtaagctgtg aaaacatgca aatttcaatt ctcgaaaaat agctgtttct
     9661 tatcaaaaat cacaccagat cagctttttt tgtatgaatt agacggggat attttttagc
     9721 atgggaatat tttgtaaatt tatgagcaaa accattaacc ttaacgttat ggatgagaag
     9781 ctaactattt tgggaattaa aattgaggac aggatgaagg aggccggcga agttcagaaa
     9841 ttactttcgc gttacggttg ctccatcaaa acccggctcg gattgcatga agttgccggt
     9901 gattactgca gcacttcggg tttaattatt ttggagctgg ccggaccggc tgaagatcag
     9961 agaaaactcc acgacgagct acagaaaatt caaggagtgc ttgtccgcaa aatggaattc
    10021 tgatttcagc tgtaaaacac tttgctttag gttgagttga aacgaatctg ctttcgtttc
    10081 aactctttta tattcctgcc ccattatggg gagcattaag aataactgaa tctgaagtgg
    10141 ttaaaaaata gtatctttgc aggctaatat tttgagcgaa taagataatg caagtagaga
    10201 aagggcagga tacaaatcgg gtaatactgg taaccaacga cgatgggtac aaatcgagcg
    10261 gaatggaagc acttatcgag atggttaaac cgctgggaag ggtagtagtg gtggctcccg
    10321 aggagggtaa ctcgggaatg tcgcatgcta tcaccattaa ggttccgttg aggatgaagc
    10381 atcgcaaccg cgacgataat gttgagcttt acaccgtaaa cggcacgccg gttgactgcg
    10441 tgaagcttgc catgaacgag ctgtttgcaa aaatacctcc cgatttgatg gtttcaggca
    10501 ttaaccatgg ttcaaatgcc tccattagcg tggtttacag tggaacaatt gctgcggcca
    10561 ttgagggttg cctttacggc ataccatccg tgggtttttc aattcttgac tacggggaat
    10621 accccgattt tagggcatca attctatacg gacgtgaaat tctaaagaat gtaatggaga
    10681 agggtatttc gccggaatgc tgcctgaatg ttaacatacc cgtgcttcct ccccaggaga
    10741 taaaaggggt aaaagtagcc cggcagaata ggggcagctg gagggaggag tttgagaaac
    10801 gcaccgaccc caggggagtt aactactact ggctcactgg ctactttcac aacgatgagc
    10861 ccgaggcaac cgataccgac gagtacttcc tttctaaggg ttacattact gttgtgcctg
    10921 ttaacgtcga ttttacagat taccgggaga tgaaaaagct tcaagggtgg gagaatgacc
    10981 tttacaatgc cgtaaaaaat gaactcagcc aagctaaata gacaacgggt aggttacctc
    11041 ttcgggattt tgctacccct gctggtgctg gtggtgattt tctttactat gtacaccggt
    11101 aaaagcttct gggcttttct ccagattatg tggattcaag gcaacctatc gcaggtgctg
    11161 tcgcttgctg ttattcccaa cctggctatt ttcttcatat tcatttggac aaaaaaaatg
    11221 aaggctgccc agggtgtgct tggggcaaca tttattcttg catttctcat cattatttta
    11281 cgttttgcac gataaaacca gctaaatgcg ctactttata gttgcaggag aagcatcggg
    11341 cgatttgcac gcctccaacc tgatgcgggg aattaaggcc aaagattctt cggccgaatt
    11401 cagattcttt ggtggcgatt tgatgaaggc tcagggagga acactggtaa agcattaccg
    11461 cgatatggcc ttcatgggct tcctggaggt actgctgaat cttcgcac
//
LOCUS       DAOPHX010000084        11283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_6959_length_11283_cov_6.245458, whole genome shotgun sequence.
ACCESSION   DAOPHX010000084 DAOPHX010000000
VERSION     DAOPHX010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 11283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11283
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6959_length_11283_cov_6.245458"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            642..905
                     /locus_tag="PK502_07265"
     CDS             642..905
                     /locus_tag="PK502_07265"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPH00652.1"
                     /translation="MEKPVLIEMFYTLSCPNCRTLKRMLDEILPTFGNKFEFKRTMAN
                     SPVGMIRTMKLGIGAVPTLLINNEIVFRQVPTKEELINKLNQY"
     gene            924..1157
                     /locus_tag="PK502_07270"
     CDS             924..1157
                     /locus_tag="PK502_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00653.1"
                     /translation="MEAQKKLRCPLGIPGGILVALIGLVGIVLNIINFDWFELLQSIA
                     LLLLGLPFVRVTMMVHLGNDRLDALEDKVNGKK"
     gene            1180..1566
                     /locus_tag="PK502_07275"
     CDS             1180..1566
                     /locus_tag="PK502_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPH00654.1"
                     /translation="MKKIIYWTSTVLISLMMLSSAWGYFTNPDMKGAFVHLGFPDYFR
                     IELGTAKFLGVLALLIPMIPARIKEFAYFGFTITFISAIIAHLSSGDPIMAVVFPLVA
                     QVLLTVSYIYFILISDKKMTMQEAES"
     gene            1654..2211
                     /locus_tag="PK502_07280"
     CDS             1654..2211
                     /locus_tag="PK502_07280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPH00655.1"
                     /translation="MIKVGIIIGSTRPGRNAEAVAKWVYEHARRRTDAEFELVDIKDF
                     NLPVLDEPVPASMHQYMKDHTKVWSDKISSFDAFIFVTPEYNHATTGALKNAIDYLYV
                     EWNNKAAGFVSYGSTGGVRAVEQLRLIMGELQVADVRTQVALSLFTDFENFSVFKPAA
                     LHEDNLKAMLDQLVAWGGALKTIRK"
     gene            2216..3034
                     /locus_tag="PK502_07285"
     CDS             2216..3034
                     /locus_tag="PK502_07285"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HPH00656.1"
                     /translation="MNGSDKLPVIFIPHGGGPWHIIPDAMGDPVGYGNLRGYLKELGN
                     SYRSRIRSILMVSAHWEETFPTIHFGESPNLYYDYYGFPEYTYHLSWPAPGNPNLALR
                     VEQLLNNKGFNTRREMIRGYDHGTFVPMMVAFPEAQLPVVQLSLVRGLDPSTHIAVGQ
                     ALEPLRSEGVLIIGSGMSYHNMQGFMSGSPEVESTSKKFDNWLAAAVEVKNPEQRIQT
                     LVKWAEAPGAFESHPRSEHLVPLFVVAGAAGSDAGKRSYNGKLMSATISGFTFG"
     gene            3104..3553
                     /locus_tag="PK502_07290"
     CDS             3104..3553
                     /locus_tag="PK502_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPH00657.1"
                     /translation="MELESEVKGHFRNEYHKGLINLIYTVNLLSYEFLQSLKNHGVTE
                     QQYNVLRILRGFRSQAPVSLGFIKERMLDRNSDVSRIIDKLQRRGLVSRDVSSCDRRQ
                     KAIQITDKGLNLLSSMLDCEKKVDLLLNNLTPDELRQLNQLLDKIRG"
     gene            3865..6453
                     /gene="clpB"
                     /locus_tag="PK502_07295"
     CDS             3865..6453
                     /gene="clpB"
                     /locus_tag="PK502_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPH00658.1"
                     /translation="MNLNNFTIKSQEAIQKAIEIAQGYNHQAVEPAHLLKAILSESEN
                     ITGYLLRKLGVNPATFDEVLDRMLDSMPKVSGGEQYLSSSTSKVLQRALEFSKSMGDQ
                     FVSVEHILLGLIVAGEQVGQMMKDAGMTEKEMKLAIEELRKGSKVNSQTAEDTYNALN
                     RFAINLNEMARSGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGVGKTAIAEGIA
                     HRIVSGDVPENLKTKQIFSLDMGALVAGAKYKGEFEERLKSVVNEVISANGEIILFID
                     EIHTLVGAGKGEGAMDAANILKPALARGELKAIGATTFEEYQKYFEKDRALERRFQSV
                     VVDEPLPADAISILRGLKERYENHHHVRIKDEAIIAAVELSHRYITSRYLPDKAIDLI
                     DEAASKLRLEMNSVPEDIDEIERRIKQLEIEREAIKREGDKAKLEDLSREIAELNEER
                     TKLRAKWQNEKSIIDRIQKNKQEIENLRLEAEEAERRGDYGRVAEIRYGRIKQAESEI
                     EQMKVELKGVQADFALIKEEVDAEDIAEVVSRWTGIPVSRMLQSEREKLLHLEEELHK
                     RVVGQDEAIAAVADAVRRSRAGLQDSKRPIGSFIFMGTTGVGKTELAKALAEFMFNDE
                     NLMTRIDMSEYQERHSVSRLVGAPPGYVGYEEGGQLTEAVRHKPYSVVLLDEIEKAHP
                     DVFNILLQLLDDGRLTDNKGRTVDFRNTIIIMTSNIGSHIIQENYASLTRSNANEVFE
                     RTRQQVFDLLKESIRPEFINRVDEIIMFTPLSHHEIKQIVMLQFNRIKDMLAQNGVKL
                     EISEAAVEWITNAGFDPVYGARPIKRVMQKYILNELSKQILAGSIDRDKTITVDYFGE
                     DVLVFR"
     gene            complement(6634..7902)
                     /locus_tag="PK502_07300"
     CDS             complement(6634..7902)
                     /locus_tag="PK502_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005805768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HPH00659.1"
                     /translation="MFALADCNNFYASCERVFDPSLNGKPVVVLSNNDGCIIARSNEA
                     KALGLKMGEPAFKIKDIIEKNRVAVFSSNYTLYGDMSQRVMNTLSGFTPEIEIYSIDE
                     AFLSLHGFTHFNLLEYGHKIRSVTTQNTGIPISIGIARTKTLAKVANHFAKKQPNYGG
                     VCLLGSEAERVEALKHFEIGEVWGIGRQYNKLLNRYGVSTAYDFTKLPTEWVRRHMSV
                     VGLRTQKELMGIPCLDLEHTAPPKKSICTSRSFGQMQTELGYLEEAVASFASSCAHKL
                     RKQQSVATLVMVFAHTNFFRDDLPQYSASKIATLPVATNSSMEIVHYALQALRSLFKP
                     GYRYKKAGVIVSGIQNSSGVQTALFDTVNRNKHSLAMAAMDIINDRFGPSTIKLAAQG
                     TGRKWRLRQEKLSPFYTTRWDQLITVNAER"
     gene            complement(7904..8344)
                     /gene="umuD"
                     /locus_tag="PK502_07305"
     CDS             complement(7904..8344)
                     /gene="umuD"
                     /locus_tag="PK502_07305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HPH00660.1"
                     /translation="MKLVKSTQTIDFYTPDLNAELTLPYADGIRAGFPSPAQDYLDLA
                     FDLNRELVKNPSSTFYGRVRGNSMVDAGINDGDILVIDKSIPPADGRRAVCFIDGEFT
                     LKTLKVEKDILWLLPANPKYKPIKVTPESNFTVWGIVTYVIHKV"
     gene            complement(8354..8722)
                     /locus_tag="PK502_07310"
     CDS             complement(8354..8722)
                     /locus_tag="PK502_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00661.1"
                     /translation="MEIEKIVETIISNAKGLTNDELTKCRSEYDPIYLNDRITHHRKI
                     SNRQNITLFLLVIFAILAFVVVVFGAKVESGTMSWIRGVVTGYFVAIIFLMPKTFKNH
                     SSIASTLSLIKTLREEQKKM"
     gene            9011..9976
                     /locus_tag="PK502_07315"
     CDS             9011..9976
                     /locus_tag="PK502_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HPH00662.1"
                     /translation="MKIAVAFRTDPDQRKYLVDELGGETELIFLNDLAEDKKMEVLSE
                     VEIILSWNPEKEGITNSKNLGSNLKFIQLMSAGYDHVNPDDFPSNVKIAANQGAYAEP
                     MAEHALAMILALSKRLPLYHQHLVKGEFNQLKSPTKSLRGSVLGIIGFGSIGKATARL
                     AKPFGVKVMALNTSGKTDEEVEYIGTLNDMEHVLRNADSLLISCALNEQTEGLINRQA
                     LELMKPDAVLVNVARGPIVVEKDLYEHLKGHPNFYAGIDAWWIEPFKYGRFELHYPFF
                     ELPNIIGSPHNSAMVENSMIIGTEKAVANLRRFINNEAVRGLINR"
     gene            9999..10733
                     /locus_tag="PK502_07320"
     CDS             9999..10733
                     /locus_tag="PK502_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HPH00663.1"
                     /translation="METHSTTSNNIKVEIWSDVVCPFCYIGKHRFESALEKFQHSNIV
                     EVEWKSFQLDPYAVTNPNLSVMDNLAREKGLSAAQVQESVGYIADMAKGVGLDFHFEK
                     AIVANSFKAHRILHLAKTHGKQNELEEKLFAAYFTQGMNIDDDDVLLNLATSVGLSGD
                     EVRKVLGSDAYSDEVKHDIAHARQLYVRGVPYFLFDEKYAVSGAQEVDFFLQTLNKAY
                     ADHKGPEVIEIPGNNPGVCGPDGCSI"
     gene            10753..11229
                     /locus_tag="PK502_07325"
     CDS             10753..11229
                     /locus_tag="PK502_07325"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPH00664.1"
                     /translation="MDKPRSFEIKSQSKGIQLPDDVIPFDLKHVSDSFVSGRQQRYIE
                     IQYFRGSKPENFYARVLFRKEAQGAPDIVHGGAIAAVFDETMGAICLVLGQPAVTATL
                     TVDYRIPVPVEKEFLVEARLEKTERRKFFITARMLDEQQELFAESKAIYVKTKNDG"
BASE COUNT         3056 a         2390 c         2843 g         2994 t
ORIGIN      
        1 tgttttaagc cccgaagggg cggaataagt agcaaggggc gcagcccctt gtatgaagcg
       61 aacgattttt acaagccctg aaggggcgga ataggtttga aacgaagagc ctgaagagga
      121 tgtgttggcg atgcaagtag tggcttttcc aaagagctgt tcagaaccgt tacatcgcct
      181 tcacaaaatt gttgcaaaga gtcatctaag tgttctttct tgtcttgata caagaaagaa
      241 ccaaagaaaa tcatgtccga aaagccagac ccatttgcgg gtcaggcttt tatgccattt
      301 tactaatacc agcaatgttc aacgattccc ggctttacga gcactgcgga gtataccctt
      361 ctatggtcag agttattcag aataagctgg agtatgagga ggttagatga agcaagatga
      421 agctataggg ggtgaagata gccatttaac cgtcaggaac gacgagggaa agcttaacac
      481 tttctctctc gttcttgaaa aattagtttt ttttcgtttg ccccttaagc taacgcccta
      541 aaaagttgca tttgcaatca aaatctaagc aaaaaaatta atggatgttg caacaaataa
      601 ctacttggct tgttactatg acaaaaattt aaatgaaaac aatggaaaaa cccgttttaa
      661 ttgaaatgtt ttacacgctc tcttgtccca actgtcgcac actaaagcga atgctggatg
      721 aaattctccc aacttttggg aataaatttg agtttaagcg caccatggcc aactccccgg
      781 ttggtatgat tcgaaccatg aagcttggta taggtgctgt tccaacattg cttatcaaca
      841 acgagattgt ttttaggcag gttccaacaa aagaggagct tataaataaa ctaaatcaat
      901 actaacctta aattttaatt acaatggaag cacaaaaaaa acttcgctgc ccactaggta
      961 ttcccggcgg aatattagta gccctaatcg gattggtggg aattgttcta aacattatca
     1021 acttcgactg gtttgagctg ctacagtcaa tcgccttact gctactcggc ttaccttttg
     1081 ttagggtaac catgatggtt catttgggca acgataggct tgatgcactt gaggataaag
     1141 taaatggtaa gaaataatca aggaagaaat attatgaggg tgaagaaaat tatttactgg
     1201 acaagcaccg tgctcatatc gttgatgatg ctgtcgagtg catggggata ttttactaat
     1261 ccggatatga agggagcgtt tgtccacctt ggatttccgg actacttcag aatagagttg
     1321 ggaacagcaa aatttctggg agttttagcc ctgctgattc ctatgattcc tgctcgaatt
     1381 aaggaatttg cctactttgg gtttaccatt acctttattt cggccataat tgcgcatttg
     1441 tcgagtggtg atccaattat ggcagttgtt tttccgctgg ttgcccaggt actgcttacc
     1501 gtatcctaca tctacttcat cctgatttct gataaaaaga tgacaatgca ggaagccgag
     1561 agctaagcaa ataatgatat gttgccctgg ttatctgggc aatagtgaat acagaacggt
     1621 ttaattttaa taacactaaa caatcttgaa gtaatgataa aagttggaat tattattgga
     1681 agcacaagac ctggcaggaa tgccgaggct gttgccaagt gggtttacga acatgcccgc
     1741 aggagaaccg acgctgagtt tgagctggtt gatattaagg acttcaacct tcctgttctc
     1801 gatgagcctg ttcctgcctc catgcaccag tacatgaagg atcacactaa ggtgtggtca
     1861 gataaaatca gctcgtttga cgcttttata tttgtaaccc cggagtataa ccatgccaca
     1921 accggagcgc taaagaatgc aattgactac ctctacgttg agtggaataa taaggcggca
     1981 ggttttgtca gctatggttc aaccggcggg gttcgggctg ttgagcagct tcgtctcatc
     2041 atgggtgaac ttcaggtggc cgacgtgcgt acccaggtgg ccctgtcgct ttttaccgat
     2101 tttgaaaact tcagcgtttt caaaccggct gcacttcatg aggataacct caaagccatg
     2161 cttgatcagc tggtggcttg gggcggtgct cttaaaacca ttagaaagta ggattatgaa
     2221 tggaagcgat aaacttccgg tgatattcat tccacacggg ggaggtccat ggcatataat
     2281 acccgatgcc atgggcgatc ccgttggcta cggaaacctt agaggctacc ttaaagaatt
     2341 ggggaattca tacaggagca ggattcgatc cattctgatg gtttctgctc actgggaaga
     2401 aacgtttcca accatacatt ttggcgaatc gccaaatcta tactatgatt attacggttt
     2461 cccagaatac acctaccacc ttagctggcc tgcaccgggt aatcctaacc tggcactgag
     2521 ggttgagcag cttcttaaca acaaggggtt taacacccga cgagagatga tcaggggata
     2581 cgaccatggt acatttgtac ccatgatggt tgcatttcct gaagctcaac ttcctgttgt
     2641 tcagctttcg ttggtccgtg ggcttgaccc ttccacccac attgcggttg gacaagccct
     2701 tgagccactc cgttctgaag gagtgcttat catcggctcg ggcatgagct accacaacat
     2761 gcagggcttc atgtcgggct ctccggaggt agaatccacc tctaaaaagt ttgacaattg
     2821 gctggccgca gccgttgagg ttaaaaaccc tgaacaacga attcaaaccc ttgtaaaatg
     2881 ggctgaagcg cctggcgctt ttgagagcca tcccagaagt gagcacctgg taccactttt
     2941 tgttgttgcc ggtgcagcgg gtagtgatgc aggtaaaaga agctacaacg gaaagctgat
     3001 gagcgccacc atttcgggct ttacatttgg ttaataatcc ttgggcaagc ataagactaa
     3061 attgtggcgt cttttttttc agaaacttaa tcctctttaa aacatggagc tggaaagcga
     3121 agtaaagggc cattttagga acgaatacca taagggactt ataaatttga tttatacggt
     3181 gaacctgctg agctacgagt ttctccagtc gcttaaaaac catggagtta ccgagcagca
     3241 gtacaacgtg cttagaattt tacgtggctt tagaagccag gcacccgtat cgcttggatt
     3301 tattaaggaa cgaatgctcg ataggaattc tgatgttagc cggattattg acaaacttca
     3361 aaggcggggc ctggtatcca gagatgtcag cagttgcgac agacgccaga aagccattca
     3421 gattactgat aagggtttaa acttgcttag cagcatgctc gactgtgaaa aaaaggttga
     3481 cctgttacta aataatctca ctcccgacga attgaggcag cttaatcagc tcctcgacaa
     3541 aattaggggc tgacctgccc taaatcatac acttcccgac tgtttttctt attctgtatc
     3601 agaaaacttt ttgaacgcca tccatactgg ctgttcatta tactggaacc gtttaaaccg
     3661 taaaatcccg aagtgggctt acggttgttt tttattataa tgtgcttgaa tatagtgcat
     3721 tacacaaaat tagggatatt cctgatttct ccgcactgtc ataatttcct atttgcgctt
     3781 tgtgcttatg tcaacatggc attatttggg ttatggtaga tgttttgctg gcttatggtg
     3841 ataaatttaa ggaggaacta tagtatgaac ttgaataatt ttaccataaa atcgcaggag
     3901 gccattcaga aggccattga gattgcgcag gggtacaacc atcaagctgt agaacctgcg
     3961 cacttgctaa aggctatttt gagtgagtcc gaaaacatta ccgggtacct tcttcgaaag
     4021 ctgggtgtaa accctgcaac atttgatgag gtgctggacc ggatgcttga ttccatgcca
     4081 aaggtttcgg gtggcgagca gtacctaagc tcgtcaacca gcaaggtgct gcaaagggct
     4141 ctggaattca gcaagtccat gggcgaccag ttcgtgtccg tggagcatat cctgttgggg
     4201 ttaattgtgg ccggtgaaca ggttggacag atgatgaagg atgccggaat gaccgaaaag
     4261 gagatgaagc tggctattga ggagttgaga aaaggatcaa aggtcaattc acaaacagcc
     4321 gaggacacct ataatgcgct taacagattt gccattaacc tcaacgagat ggcgcgaagc
     4381 gggaagctcg atccggtgat tggtcgtgat gaggagataa ggcgcgtgct gcagattctc
     4441 tcgcgtcgta ccaagaacaa tcctattctg attggtgaac caggcgttgg gaaaactgcc
     4501 attgccgagg gaattgccca ccgtattgtt agcggcgacg tgcccgaaaa cctcaagacc
     4561 aagcaaattt tttcgctcga catgggggcg ctggtggccg gcgctaaata caagggcgag
     4621 ttcgaggagc ggctaaaatc ggtggtaaac gaggtgattt cggctaatgg agaaattatc
     4681 ctgtttattg atgagataca caccctggta ggtgctggta aaggtgaggg tgctatggat
     4741 gccgccaata tccttaaacc ggcattggca aggggcgagt tgaaagctat tggagccacc
     4801 acttttgagg agtaccaaaa gtattttgaa aaggacaggg cgcttgagcg tcgtttccag
     4861 agcgtagtgg ttgacgaacc ccttcctgcc gacgctatct caattctgag agggctaaag
     4921 gagcggtacg agaatcatca ccatgtgaga attaaggacg aggccattat tgcagccgtt
     4981 gagctgtcgc atcggtacat caccagccgc tatttgcccg ataaggccat agacctgatc
     5041 gacgaggccg cctcaaagct gaggttggaa atgaattctg ttccggagga tatagacgag
     5101 atagaacgtc gcattaagca gttggagatt gaacgtgagg caataaaacg tgagggcgac
     5161 aaggccaaac ttgaggactt atcacgcgaa attgccgagc taaacgagga acgcacgaag
     5221 ctaagagcca agtggcaaaa cgagaaaagc ataattgaca ggattcagaa aaacaagcag
     5281 gagattgaga accttcggct cgaggctgag gaggctgaac gtaggggcga ttacggaagg
     5341 gtggctgaaa tccgttatgg cagaataaag caggccgaat cggaaattga gcagatgaag
     5401 gtggagctga agggcgttca ggccgacttt gccctgataa aagaggaggt tgatgcggag
     5461 gacattgccg aggtggtttc gcggtggacg ggcattccgg taagccgcat gctgcaaagt
     5521 gagagggaaa aactacttca cctggaggag gagctgcata agcgagttgt tggtcaggat
     5581 gaggcaattg cggctgttgc cgatgcagtt aggcggagcc gcgcagggct gcaggattcc
     5641 aagcgaccta tcggatcgtt catcttcatg ggaactacag gcgttggaaa aaccgagctg
     5701 gccaaggctc tggccgagtt catgtttaat gacgaaaatt tgatgacccg tattgacatg
     5761 agcgaatacc aggaaaggca ctccgtatca aggctggttg gtgcacctcc cggatatgtt
     5821 ggctacgagg aaggtggaca gcttaccgag gcggttcggc ataagcccta ctcggtagtc
     5881 ttgcttgatg aaattgagaa ggctcatcct gatgtattca acatactgct tcagctgctc
     5941 gacgatggcc ggttgactga taataaggga cgaactgttg acttcaggaa caccatcatc
     6001 atcatgacgt caaacatcgg ttcgcacatc atccaggaaa actacgcttc gcttacacga
     6061 agcaacgcca acgaggtgtt tgagcgaacc cgtcagcagg tgttcgacct gctaaaggag
     6121 tccattcgtc cggaattcat caacagggtt gacgagatta tcatgttcac cccgcttagc
     6181 catcacgaga ttaaacagat cgttatgctg cagtttaacc gtataaaaga tatgctggct
     6241 cagaacggtg ttaaacttga gatctccgag gcagctgtgg agtggataac caatgccggt
     6301 ttcgacccgg tgtacggcgc tcgtcctata aaaagggtga tgcagaagta catccttaac
     6361 gagctttcta agcaaattct tgctggcagc attgacaggg ataagaccat tacggttgat
     6421 tactttggtg aggatgtgct ggtgttcaga tagtttttag gctagtttca tatttagcaa
     6481 tacctatagc cggaataacc ctcagggcaa ttccggcttt ttggtatggc acatgcgtta
     6541 agggaaaatg gcctgttacc atttcctttc caaaatttgc tgttgaggtt ttaccgtgtt
     6601 cgtccttacg cttcatggtc aggttctact tcttcacctt tcagcattaa ccgtgataag
     6661 ctggtcccag cgtgtggtgt agaagggcga tagtttttcc tgtcttaggc gccattttcg
     6721 tccggttcct tgggctgcaa gtttaattgt gctggggcca aaacgatcgt taataatatc
     6781 catggcagcc atagctaggc tatgcttatt gcggttgacg gtgtcaaaca gggcggtttg
     6841 caccccgcta ctgttctgga ttccactaac aattacccct gcctttttat accggtaccc
     6901 tggtttgaat aggctacgaa gcgcctgcag ggcgtagtgg acaatttcca tgctgctgtt
     6961 ggttgctacc ggtagggtag ctattttgct ggcggagtac tggggtaaat catcgcggaa
     7021 aaagttggtg tgggcaaaaa ccattaccag cgtggcaacc gattgctgct tacgtagctt
     7081 atgcgcgcag ctgctggcaa atgaggcaac ggcctcctcg aggtaaccca gttcggtttg
     7141 catctgaccg aatgatcgtg aggtgcaaat ggactttttg ggaggggcag tgtgttcgag
     7201 atccaagcac ggaataccca tcagctcctt ttgggttctt agtccaacaa ccgacatgtg
     7261 gcgccttacc cattcggttg ggagtttggt aaagtcgtag gctgtactaa caccgtaccg
     7321 gttaagaagc ttgttatact gccggccaat gccccaaacc tccccaattt caaaatgctt
     7381 caatgcttca acccgttcag cctcgcttcc cagcaggcat acgccgccgt agttaggctg
     7441 ctttttggca aagtggttcg ccacctttgc cagggttttt gtgcgggcaa tgccaatact
     7501 tatcgggata ccggtgtttt gagtggtaac gctacgtatt ttatggccat actccaacag
     7561 gttgaaatgg gtaaatccgt gcaggctcag gaaagcttca tcaatggagt aaatttcgat
     7621 ttccggggta aagccggaca gcgtattcat caccctttgg ctcatatcgc cgtagagcgt
     7681 gtagtttgat gagaaaacgg ctaccctgtt tttctcaatg atatctttga ttttaaaggc
     7741 aggctcaccc atttttaatc ccagggcttt tgcctcgtta cttcgggcaa ttatgcagcc
     7801 atcgttgttg gagagtacca ctacaggctt gccgttaagc gaagggtcga aaaccctttc
     7861 gcatgaggcg taaaagttgt tgcagtcggc cagagcaaac atactaaacc ttatggatta
     7921 cataggttac aataccccaa accgtgaagt tgctctcggg cgtaacctta atgggtttgt
     7981 acttagggtt agccggcagt aaccaaagta tatccttttc aaccttaagg gttttgaggg
     8041 taaactcacc atcaatgaag caaaccgccc ttcggccatc ggctggcgga atagatttat
     8101 caataaccag aatgtctcca tcgttgattc ctgcatcaac catcgagttt ccacgcaccc
     8161 tgccgtaaaa tgtggagctt gggtttttaa ccagctcccg attcaggtca aaggctaagt
     8221 cgagataatc ctgtgcgggc gaggggaatc ctgccctaat gccatctgca tacggtagcg
     8281 ttagctcggc gtttaagtcg ggtgtgtaaa aatcaatggt ttgtgttgat tttaccagct
     8341 tcatgggttt atgctacatc ttcttttgtt cctcacggag cgttttgata aggcttagcg
     8401 tgctggcgat tgaggagtgg tttttgaaag ttttgggcat caggaatata atggccacga
     8461 agtaaccggt tacaacaccc ctaatccagc tcattgttcc cgattctact tttgccccaa
     8521 aaacaacaac cacaaaggca agtatggcaa aaattaccag caggaaaagg gtgatatttt
     8581 gccggttcga aattttgcga tggtgggtta tcctgtcatt caggtagatt gggtcatatt
     8641 ccgatctaca tttagttagc tcatcatttg ttagaccttt cgcattcgaa attatggtct
     8701 ccacaatctt ttctatttcc atagcagctt tagttttgat taacaaattt actcctattt
     8761 ttggtatgcc gaattatttc tggcgaacaa taacaaacct gtaatgttta caagtcattg
     8821 gtggtagaaa ggatggaact ccatggaagc ttcctgcaag gaatttcttt acgattatat
     8881 tgaaggcgtt ctaacctaag tagtaatgct gtttacaggt gttattatga gcgtatgtaa
     8941 tgtataaatg tgattttcaa ggccaagttt gtaaagcatt aattgttagt atagtaaata
     9001 ttttgtaagg atgaaaattg cagttgcctt tagaactgat cccgaccaaa ggaagtacct
     9061 ggttgatgag ctcggtgggg aaacggagct aatttttctg aatgacttag cggaggataa
     9121 aaaaatggaa gttctttcgg aagtcgaaat tattttatcg tggaatcctg aaaaagaggg
     9181 aatcactaat agtaagaatt taggtagcaa cctgaaattt attcagctga tgtcggcggg
     9241 ctacgaccat gtaaaccctg acgactttcc ctccaacgtt aaaattgctg ccaaccaggg
     9301 tgcctatgcc gagccaatgg ctgagcatgc attggccatg atactggcat taagcaagcg
     9361 cttgccgtta taccatcagc acctggttaa gggtgagttc aaccagctaa aaagccctac
     9421 taaatcgctt agaggctcgg tgctggggat tatcggtttt ggctcaattg gcaaggctac
     9481 cgcaaggttg gctaaaccat ttggtgtaaa agtaatggcc ttaaacacct cggggaaaac
     9541 cgacgaggag gtggagtaca ttgggacgct gaatgacatg gagcatgttc tcaggaatgc
     9601 cgatagcctg ctaatttcat gcgcacttaa cgagcaaacc gaagggttga ttaaccgtca
     9661 ggcacttgag ctgatgaaac ccgatgccgt gctcgtaaat gttgcccgcg gcccaatagt
     9721 tgttgagaag gacctatacg agcacctgaa aggccatcca aatttttacg caggtatcga
     9781 cgcctggtgg attgagccct ttaagtatgg taggtttgag ctgcactatc cgttttttga
     9841 acttccaaat attattggtt caccccataa ctcggccatg gtggaaaatt ccatgattat
     9901 tggaaccgaa aaagccgtgg ccaacctccg gaggtttatt aacaacgagg ctgttagagg
     9961 acttatcaac aggtagagta ttaaccataa ataaaaaaat ggaaacacat agcactacta
    10021 gtaataacat caaggttgaa atttggagcg atgtggtttg ccccttctgc tacattggca
    10081 agcataggtt tgaaagcgcc cttgagaagt ttcagcattc taacatcgta gaggttgaat
    10141 ggaagagctt ccagctcgac ccgtatgcgg ttactaaccc taaccttagc gttatggaca
    10201 acctggcccg ggagaagggt ctgtcggcag cacaggttca ggagtcggtt ggctacatcg
    10261 ccgatatggc gaagggcgtt gggctcgact ttcattttga aaaagcgatt gttgctaact
    10321 cctttaaggc gcacagaatt ctacatctgg ccaaaaccca cggaaagcaa aacgagctgg
    10381 aggaaaagct gtttgcagcc tacttcaccc aaggtatgaa tattgatgac gacgatgttt
    10441 tgctgaacct ggccacatcg gtggggctaa gtggagatga ggtgcgcaag gtgctgggta
    10501 gcgatgcata ctccgatgag gtgaagcacg atattgccca tgcacgtcag ctttacgtta
    10561 ggggcgtgcc ctactttttg tttgatgaga agtatgccgt ttctggggct caggaagtgg
    10621 atttttttct tcaaacgctc aacaaggctt atgccgacca taagggtccg gaggtgattg
    10681 aaattccagg caacaacccc ggggtatgtg gtcctgatgg gtgcagtatt taaataaatt
    10741 aggcagtgaa ccatggacaa acctagaagt tttgaaatca aaagccagtc aaagggaatc
    10801 cagctgccgg atgacgtgat tccttttgat ttgaaacacg tttcggattc gtttgtttca
    10861 ggaaggcagc agcgctacat cgaaatccag tactttcggg gtagcaaacc cgaaaacttt
    10921 tacgccaggg tactcttccg caaggaagct cagggtgcgc ccgacattgt tcatggtggc
    10981 gccatagccg cagtgttcga tgaaaccatg ggagccatat gcctggtgct gggccagcca
    11041 gctgttaccg caaccttgac ggtagattac cgaatacccg tacccgttga aaaggaattt
    11101 ttagttgagg cacggcttga aaagaccgaa aggcggaagt tctttattac agccaggatg
    11161 ctcgatgagc aacaagaact tttcgccgaa tcaaaggcta tctacgtgaa aacaaaaaac
    11221 gacggatgac ccgtcaaaat tttctgttgc atttccgtcg gtttaagata ggccgctata
    11281 atc
//
LOCUS       DAOPHX010000085        11237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_7000_length_11237_cov_5.051780, whole genome shotgun sequence.
ACCESSION   DAOPHX010000085 DAOPHX010000000
VERSION     DAOPHX010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 11237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11237
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7000_length_11237_cov_5.051780"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PK502_07330"
     CDS             complement(<1..546)
                     /locus_tag="PK502_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00665.1"
                     /translation="MKIAEKILTFFTLKIERKTARDSADRLVQLSIVTIPFLLYLVVF
                     CTLLLFRHQVASGIIAGVAAISILLIIVLAYSKKRVNSFGLVALAISGIALLAILIVG
                     KLDAALMIGVAMFPMLALSTTTPNRAHIISFIFWILYSCILITGPKFIGESVSSTGYY
                     RLLSIIFYPLLQLVASLLYLFK"
     gene            complement(925..1539)
                     /locus_tag="PK502_07335"
     CDS             complement(925..1539)
                     /locus_tag="PK502_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPH00666.1"
                     /translation="MGLAASIPLGPVGVVCVQRTINKGRISGFFSGLGAATTDTIFAA
                     IGGFSLTFIIGFIEAKQQIIELAGGIIIILLGLRTFYTNPVTQLKRHKKKPNTLVEDF
                     ISMFLLTGANPFTIFLFIALFAALGIVTKNQNLLFTATALVGVFLGASLWWFTLSSLV
                     NLFRHKFRLKQLWWMNKITGSIIFILGIIAIVGVVNKAWFGQLP"
     gene            1804..3429
                     /gene="lnt"
                     /locus_tag="PK502_07340"
     CDS             1804..3429
                     /gene="lnt"
                     /locus_tag="PK502_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPH00667.1"
                     /translation="MRKFYNPNFCRVFFSGILLALPWYEGLPSYSIFFGFVPLLLAYD
                     KLNEKGAGFWKLMLYAFIAFFTWNALSTWWIYKATMLGAILAVLITTMLMTLVFAGYH
                     FVRKYAGKVAGIFGFITLWIAYERFFHDSEVAWPWLSLGNSLGNHPKVIQWYEFTGMM
                     GGSLWILVINFLILGLVHAIRGKQIRRAGWFSIAALLTVILPLWISISMYNRYHEVQN
                     PVRVVIVQPSIDPYNEKFSGLTNEQQLDIMFSLAKQAADSTTDYVVCPETAIDDRIWE
                     HELHVNSSILRIKQFVDSLPNVKWVTGAITLKLFSSSENLPPTARELPYREGYFYDRY
                     NSAIQVDTAFQLPIYHKSKLVVGVEMMPYPKQLKFLEKLAIDLGGISGSLGTQPDRGV
                     LWSPDRRFGVGPIICYESDFGEFVSGYVKNGANLLFVITNDGWWGDTPGYKQHLSFSR
                     IRAIETRRSVARSANTGISAIINQRGDLVRTLGWWERGTISGTINANSKQTWYVREGD
                     YIGRLSAFMAVLTLAAALAAYLMKNNGRSVKRS"
     gene            complement(3634..5658)
                     /locus_tag="PK502_07345"
     CDS             complement(3634..5658)
                     /locus_tag="PK502_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPH00668.1"
                     /translation="MKRNIIQRGITMVLLTAGSISAFGQVEDPVQWSISSKKIDAKKY
                     ELIITATIEQGWHLYSTKLPEGGPLPTSFNFADSKDYKLVEGVKELTKPKTEHDDIFN
                     LDLSFFEGEAQFGQIVDLAGDEASINLTVEYQACYADKCIYLEKDLSHKLGKNGSVVL
                     AGEEVQPEKTTTATLAPTNDESAKKSSSSLWVFFFVSFAAGLAGLLTPCVFPMIPMTV
                     SFFMGKQGNRMNAILNAIVFGVSIIVIYTSLGLLVSLFNLGPDFTTTLTTHWITNLIF
                     FLLFIAFAASFFGLFEIVLPGSVANKTDSKADKGGFAGSFFMALTTVIVSLSCVGPIV
                     GALLVEAASGLGAKPIVGMFGFSLAFSIPFTLFAIFPSWLNSLPRSGGWMNSVKVVLG
                     FIVLAFSLKFLLTIDQSYNLNFLSRDIYLAIWIAIFTLLGLYLLGKIKFKFDSDLQHV
                     GVLRLFLAIASFTFVVYLIPGMFGAPLKAISGLIPPQSSQKFSLNQNSQAVMPTVNDN
                     TNLSLCDQPKYADIFKLPYNLKGYFDYNQALECARKLGKPIFVDFVGHTCANCKDMEA
                     RVWSDPRVLERLNSSYIIVALYVDDRTELPESEWVTSTRDGKVKKTIGKKNSDFQATR
                     FNVNGQPYYVLLGTNEQMLTEPMGYNTNVDEFVAFLDKGVEEFNKSTTVK"
     gene            6025..7539
                     /locus_tag="PK502_07350"
     CDS             6025..7539
                     /locus_tag="PK502_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPH00669.1"
                     /translation="MKLFTTKQIRELDAYTIKHEPISSYELMERAANALYSWFAQNLS
                     TLQPIVVFCGPGNNGGDGLALARLLYTSGYSVEVYYLTASAHSSDFQLNLERLKAINF
                     LPVEIFSNSGFPHIGENQVVVDAIFGSGLSKPIEGLAAQLVSHINTSGSRVVAVDIPS
                     GLFGEENPFPNSNPVVRAADCLTLQFPKLSFFFAENSSFVGRWHVLPIGLHPEAIAGA
                     STSWHYTNMETIRGMVKPRERFAHKGIFGHCLVVAGSHGMIGAAVMAAMSAARAGAGL
                     VTLHVPACGYEIAQTLAPNIMVVCDSNEHHLSAVDDFQRYNAVCIGPGRGQHPETADA
                     LGKILAEINVPLILDADALNLVAANPKLLGAIPAGTIITPHPGEFDRLFGVSTCGWKR
                     LERAREVAQRYQQVIILKGAYSQVVLPDGRVFFNSTGNPGMATGGSGDVLSGIVVGFL
                     AQGFSIADAALLATFVHGLAGDIAASDLGENALVATDMISYISNALLEIEISNL"
     gene            7559..8656
                     /locus_tag="PK502_07355"
     CDS             7559..8656
                     /locus_tag="PK502_07355"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HPH00670.1"
                     /translation="MRFSSMMVGLFLGLTMLSCSNKMATVSPVRSAPPVKDYSFIYTL
                     PRTVILIKVDACRSVFYPGPYAAYAWKYLGISDVPTLKSEQWTITDITIKGISESDPS
                     MLFAVFTDGHVPDALVQMTEAGLVIPENRAVIVSMMSKDLRRIENQAIPFSDLSVEPF
                     IGEEKNILYTKVQRDSSFVRVPIQKSMIVEKNADEKAGEAADLIFNLRKRRLEFLTTD
                     VDQPLEGNAVHVILDEIYRLENEYLSLFLGKTAVDTISKTYLFTPSGENNEKAIAFRF
                     SPTKGLLAQNDLSGSPMLVEVEPEPVPDSSEPLLATIGRLAEKQKLAQIFYRIPVVST
                     VKISDGKSEIASRRIPIYQLGPLVKIPAEFY"
     gene            complement(8673..9212)
                     /locus_tag="PK502_07360"
     CDS             complement(8673..9212)
                     /locus_tag="PK502_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HPH00671.1"
                     /translation="MRRFALLLAQLTLTAGVTIAQESSKVKWYTLEEALKLNTSEPRK
                     IFIDVYTDWCGWCKKMDSNTFNNPLIASYLTKDFYAVKFNAEGKNEITYKGQVYKNQG
                     EGQRSTHDFAIALLQGKLAYPSIAFMDGNGNLITFLSGYLTPSQIEPIMVYIMSDAYK
                     TQPWEQFASKFVSKLTSGE"
     gene            9579..11042
                     /locus_tag="PK502_07365"
     CDS             9579..11042
                     /locus_tag="PK502_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HPH00672.1"
                     /translation="MNKDIFKLEPQRLWKHFYSLTQIPRPSKKEKAAVDFMINFGRNL
                     GLETITDEVGNVIIRKPATPGMENRKTVILQGHLDMVPQKNSDKKHDFEKDPIEAYVD
                     GEWVTANGTTLGADNGIGVAAAMAVLEAKDIAHGPVEALFTIDEETGMTGAFGIKPGL
                     LKGDILLNLDSEDEGELYVGCAGGLDANIRFSFTPQPVPSDMVAYKLAVLGLKGGHSG
                     LEINLGRGNSIKLLFRFLYRACKTLGVRIGTIDGGSLRNAIPRESFAVVTVPSKNATN
                     FEKLVREYEQIYKNEFSLTEPNLSFTCKTDSLPKFLVDENSQSRAIRAFFSCPNGVVR
                     MSDAVPGLVETSNNLARVYTEGNQIVGQCLMRSSVDSAKEALADKMAAIFELADAAIL
                     FEGGYPGWKPNPNSPILTVMQQVYKNKFGTTPEVKAIHAGLECGLLGGVYPNLDMISF
                     GPTIRHPHSPDEKVNIATVGKFWEFLVEVLKNVPLKG"
BASE COUNT         3081 a         2426 c         2486 g         3244 t
ORIGIN      
        1 cttgaaaagg taaagcagcg aggcaactag ctgcagcagc gggtaaaata tgatggaaag
       61 taaacggtag tagcccgttg aactgacact ttcaccaata aatttagggc cggtaatgag
      121 aatgcacgag tatagaatcc agaaaataaa ggaaataata tgtgctctgt tgggggtagt
      181 tgttgataag gccagcatgg ggaacatggc taccccaatc attaatgcag catcgagttt
      241 accgacaatc aaaatggcca gtagggctat acctgaaata gctaacgcaa ctaatccgaa
      301 tgagttaacc cgcttcttgc tataagccaa aacgatgatg agtaatatgc ttattgcagc
      361 aacgcctgca attatccccg atgccacttg atgtctaaaa agcagcaatg tgcaaaagac
      421 aacaagatag agtaggaagg gtatggttac aatgcttagt tgaaccagtc tatcggctga
      481 atcccttgca gttttccttt caatttttaa cgtgaaaaag gttaagattt tttcagctat
      541 tttcattgct ttaagaaaat tgagtaaaga ttgctaccaa aggtagttta aaaaatgtaa
      601 aagcaataat cggcacgagc agaattttac ctgtatcttt tatgcttccc tcgcggtgct
      661 gccgcagaac aaccctaata gaaaaatgta aagctgctta ttcaacataa tgaggcctaa
      721 tctttaatga cttggaatac tcaggtaaag gtaggtaacg cctataatat acaaacccga
      781 tggtttgcgc tttacaatcc atgccgttga aaacaaaacg cactattggt gattgcctga
      841 ttggcttcac aaaatccccg tgcatctggt aataaaagat tgtattaacg gatgaaagat
      901 ttggatggcc agggaaaggc agcatcaggg aagttgtcca aaccatgcct tattaaccac
      961 tccaacgata gcaattatgc caagaatgaa aataattgaa ccggttatct tgttcatcca
     1021 ccagagctgc ttgagccgga acttatggcg gaaaaggttt accaggctgc taagggtaaa
     1081 ccaccacaag cttgccccta aaaaaacgcc aaccagtgcg gtagccgtaa aaagaaggtt
     1141 ttgattcttt gtaactattc ccagggcagc aaacagggca ataaaaagaa aaatggtaaa
     1201 tgggttggct ccggtaagca agaacatgga gataaaatcc tcaacaaggg tgttgggctt
     1261 ctttttgtgt cgcttaagct gggtaaccgg attagtgtag aatgttctta acccgagtag
     1321 gataataata atcccccccg caagttcaat gatttgctgc ttggcttcaa taaatccaat
     1381 aataaaggtc aagctgaacc cgccaattgc tgcaaatata gtatccgtgg ttgcagcacc
     1441 taaacccgag aaaaatccgc ttatccttcc tttgttaatt gtacgctgaa cgcataccac
     1501 accaacaggt ccgagcggaa ttgaagcggc aagccccatt atgattcctt ttagaagcag
     1561 gctaaaaatc attagatgtc ccttcaatat tacaactaca tacaagttgg caaatataat
     1621 aaatatagtc taggttaggc ttgaggctaa agctgattaa ccaaaattat gactttattc
     1681 attgaccaaa aattcattgg gctcttttga ggctttaatt attaaatggt cattttcact
     1741 agtgaagaca ggatatacaa ataatcttat ctttacaccg aactaaaatt tttatttgta
     1801 tcaatgagaa aattctataa cccgaatttt tgcagggtgt tttttagtgg gattttgcta
     1861 gctctgccgt ggtatgaagg tctccctagc tactcgattt tttttggttt tgtccccctg
     1921 ctgctggctt acgacaagct aaatgaaaaa ggtgcaggtt tctggaagtt gatgctttat
     1981 gcatttatcg ccttttttac atggaatgca ttgtctacat ggtggattta caaagctacc
     2041 atgttgggtg ccattcttgc ggtgctaatt accaccatgc ttatgaccct tgtctttgcc
     2101 ggctaccatt ttgtccgtaa gtatgctggc aaagttgccg gaatatttgg ctttataaca
     2161 ctttggatag cctacgagcg tttttttcat gactctgaag ttgcctggcc ctggctatcg
     2221 ttgggaaata gccttggaaa ccatccaaag gtaattcagt ggtatgagtt tacaggtatg
     2281 atggggggct cgctttggat tctggtgatc aattttctta tactggggtt ggttcatgcc
     2341 ataaggggga agcaaattcg tcgtgctgga tggttttcta tagcagcatt actaaccgtt
     2401 attcttccac tgtggatatc catttcgatg tataacaggt atcatgaggt tcagaaccct
     2461 gtccgggtgg ttattgttca gcccagcatc gatccctaca acgaaaagtt tagcggctta
     2521 accaacgagc agcaactcga tattatgttc agcctggcaa aacaggctgc tgacagcact
     2581 accgattacg tggtgtgtcc cgaaacggcc attgatgata ggatttggga gcatgagcta
     2641 catgttaact ccagcattct tcgaataaag caatttgtcg atagccttcc aaacgtaaaa
     2701 tgggttacag gggcaataac gcttaaacta ttcagctctt ctgaaaacct gcctccaact
     2761 gcacgtgaat taccatacag ggagggctat ttttacgatc gctacaattc agccattcag
     2821 gtcgatactg cttttcaact ccccatttac cacaaatcga agctggttgt gggcgtggag
     2881 atgatgcctt accccaagca gctcaagttt ctggagaagc ttgctattga cctgggtgga
     2941 atatctggca gcttgggtac tcaacccgac aggggtgttt tatggtcgcc cgacaggcgt
     3001 tttggtgttg gccccattat ctgctacgag tccgattttg gcgagttcgt gtcgggctac
     3061 gtaaaaaatg gagcaaacct cctgttcgtt atcaccaacg atggttggtg gggtgatacc
     3121 ccagggtaca agcagcacct ttctttttcc aggattagag ctatcgagac gcgccgttca
     3181 gttgcccgtt cggccaacac agggatttct gccattatca accaacgggg tgatttagtc
     3241 cgtacgctgg gatggtggga gcgtggcact atttcaggaa caatcaacgc aaatagtaag
     3301 caaacatggt atgtaagaga aggcgactac atcggtaggc tgtctgcatt catggcagta
     3361 cttacccttg ctgcagccct tgcagcttac ctaatgaaga ataatggcag gagtgttaaa
     3421 cggtcataat cctctaattc tatttcaagc aatagtattt gtattgctgc acttctagta
     3481 ttgttcgctt tatgcagttt aataacttaa tgcttgcaca gcctgcattt tttgtttcta
     3541 aatacacacc acttggcttc ctgtttggtg cggatgcgaa attcagaatt tatctcgggt
     3601 tttaagatct ctattttggc aatgaggcta gtgctatttt acggttgtag atttattaaa
     3661 ttcctctacc cctttgtcta ggaatgcaac aaattcatca acgttagtat tgtatcccat
     3721 aggttcggta agcatctgct cgttggttcc cagcaatacg tagtatggct gtccgttaac
     3781 gttgaaccgt gttgcctgaa aatcggaatt cttttttcca atggtttttt tcaccttgcc
     3841 atcacgggtt gaggtaaccc attcactttc gggaagttcg gttctatcat caacatagag
     3901 ggcaacaata atgtagctgc tgtttaagcg ctccagcacc cgagggtcgc tccacaccct
     3961 ggcttccata tccttacagt tagcacaggt gtggccaaca aaatcaacaa aaattggctt
     4021 gcccaacttc ctggcgcact ccagcgcctg attgtaatcg aaatacccct ttaagttgta
     4081 gggaagctta aaaatatctg catactttgg ttgatcgcaa agcgacaagt tggtgttgtc
     4141 gtttacagtg ggcatcacag cctggctatt ctggttaaga gagaatttct ggctgctttg
     4201 tggaggtata agaccggaaa ttgcttttag aggtgcacca aacatgcccg ggataaggta
     4261 aactacaaag gtaaagctgg caatagccaa aaataacctt aacacgccaa catgttgaag
     4321 gtcggaatcg aacttgaatt ttatttttcc tagtaggtaa agtccaagca gggtgaatat
     4381 ggctatccat attgccaggt atatgtcacg acttaaaaag ttaaggttgt acgattggtc
     4441 gatggtgagt agaaacttta aggaaaaagc cagcacaatg aaacccaaca ccaccttcac
     4501 ggagttcatc catccacccg agcggggtag cgaatttagc cacgatggga aaatggcaaa
     4561 tagtgtaaag ggaattgaga aggccaacga aaatccaaac attccaacaa taggtttagc
     4621 acctagccca gaggctgcct caacaagaag cgctcctaca attgggccaa cacatgacag
     4681 gctgactata accgttgtga gtgccatgaa aaacgagccc gcaaatccac ccttatcggc
     4741 cttactatcg gttttgttgg ctacgcttcc gggtagaaca atttcaaata gcccaaagaa
     4801 ggatgccgca aatgcaatga aaagtagaaa gaatatcagg ttggtaatcc agtgagtggt
     4861 gagcgtggtg gtgaagtcgg gtcccaggtt gaacagcgat accagtaaac ccaacgaggt
     4921 gtatatcaca atgatggaca cgccgaaaac tatggcgttt agtatagcat tcattctgtt
     4981 gccctgcttt cccataaaga aggaaacagt catgggtatc atcgggaaca cgcagggtgt
     5041 tagcaatccg gctaggcctg cagcaaacga tacaaagaaa aatacccata gggagctgct
     5101 tgattttttt gcactttcat catttgttgg tgcaagggtt gcagttgttg tcttttcggg
     5161 ttgaacctct tcaccggcaa gtactacaga accattcttt cctagcttgt ggcttaaatc
     5221 cttttcaagg tatatgcact tgtcggcgta gcaagcctga tattcaaccg taaggttaat
     5281 gcttgcctca tcacctgcaa ggtcaactat ttgaccaaac tgcgcctccc cctcgaagaa
     5341 cgaaaggtca aggttgaaaa tgtcgtcatg ctccgttttg ggtttggtta gctcttttac
     5401 cccttcaacc agcttgtagt ccttgctgtc ggcaaagtta aagctggtgg gcagaggccc
     5461 gccctcgggc agctttgttg agtacaaatg ccagccttgc tctatcgtgg ctgttataat
     5521 aagttcgtat tttttggcat caattttttt tgacgagatg ctccactgaa ccgggtcttc
     5581 tacctgtcca aatgcactaa tgctgcccgc ggtgagcaac accatggtta ttcccctctg
     5641 gataatgttt cgtttcattt gccacgtttt tggttacatg ttgaattgtg agaaaaaata
     5701 cttaagctac cttgtcgagg ttagtgaatt ttatctctat cttgctcatc acttaccggc
     5761 ttttctacaa aaatccataa ttcgtttgtg ggttacgggt taacaacttc gggccataga
     5821 aagttgtaat cattttattc gctggtgcac cagcaaaatt aaattttccc tcgtagtttt
     5881 tgttgcggca aatatataga ttttgaatcg gcttactttt tgatttttgt catttaagag
     5941 cttttgttaa gaaaaaatcg ccacattttc tacttttgta gtaccccatt gagccaaaaa
     6001 aagtcaacca gggtagcttt cagcatgaag ttgtttacaa caaaacagat tcgcgaactc
     6061 gatgcctaca ccattaagca tgagccaata tcctcatatg aattgatgga gcgtgctgca
     6121 aatgcacttt acagctggtt tgcacagaat ctgtcaaccc tgcaaccaat tgtagtgttt
     6181 tgtggcccag gcaacaatgg cggcgacggg ttagccctag ccaggttact ttacacttcg
     6241 ggctacagcg tcgaagttta ttacctaaca gcttctgcac actcttccga ttttcagctc
     6301 aatctggaga ggctgaaagc gatcaatttt ttaccggttg aaattttttc caactccggt
     6361 ttccctcata ttggtgagaa tcaagttgtt gttgacgcaa tttttggttc tggtttaagc
     6421 aaaccgattg aaggattggc agcccaactt gttagtcata tcaacacctc cggatcaaga
     6481 gttgttgccg ttgacattcc atctggtctg tttggtgagg agaatccgtt ccccaattct
     6541 aatcccgttg taagggcagc tgattgcctt acccttcagt ttccaaaact ttcctttttt
     6601 tttgccgaga actcttcttt cgtgggcaga tggcatgttc ttcccatcgg attgcatccc
     6661 gaagccatag ctggcgcatc aacctcctgg cactatacaa atatggagac catcagggga
     6721 atggtcaaac cccgggaacg ttttgcacat aagggtatat ttgggcattg ccttgtggtt
     6781 gccggaagtc atggaatgat aggtgctgcc gttatggctg ctatgtcggc tgccagggca
     6841 ggtgccggac tggttacgct tcatgtcccc gcctgtggat acgaaattgc acagacactt
     6901 gccccaaaca ttatggtggt atgcgatagc aatgagcacc acttatcagc agttgatgat
     6961 tttcaaaggt ataatgcggt ttgtattggc ccaggacgag ggcaacaccc cgaaactgcc
     7021 gatgctctcg gtaaaatcct agcagagatc aacgttccac ttattcttga tgccgatgcc
     7081 ctgaacctgg ttgcagctaa cccgaagcta ctcggggcaa tccctgctgg caccatcatt
     7141 acaccgcatc ctggtgagtt cgacaggctg tttggcgttt ccacttgtgg ctggaaacgt
     7201 ttggagcgtg ccagggaggt tgctcagcgc tatcagcagg taattattct aaaaggtgct
     7261 tactcccagg tagttttgcc cgatgggcgt gtctttttta attctaccgg aaatccgggc
     7321 atggctacag ggggaagtgg tgatgttctc tcgggtatcg ttgtaggttt tttagcccaa
     7381 ggtttttcta tagccgatgc ggcgctactt gctacctttg tacatggttt ggccggcgat
     7441 atcgctgcca gcgacctagg tgagaatgcc cttgttgcaa ctgatatgat tagctacatt
     7501 tcaaatgcac tacttgaaat tgaaatctcg aatctgtaaa tttagccaaa ctatgatcat
     7561 gcgcttttca tcaatgatgg ttggtctttt cttaggcctg actatgctct cgtgcagcaa
     7621 caaaatggca accgtttcgc ctgttaggtc tgctccacca gtaaaggatt acagcttcat
     7681 atacacctta ccccgaactg ttattttgat taaggttgat gcctgtcgta gcgtgttcta
     7741 tcctggtccc tatgctgcgt atgcctggaa atacttagga atttccgatg tgccaactct
     7801 aaaaagcgaa cagtggacca ttaccgatat cactattaaa ggtataagtg agtccgatcc
     7861 atccatgctt tttgccgtat tcaccgatgg tcatgtgcct gatgctctgg ttcagatgac
     7921 tgaggctggc ttggtaattc ccgaaaatag ggctgttatt gtgtccatga tgagtaaaga
     7981 tttaaggaga atagaaaatc aggctatacc tttctccgac ctatcggttg aaccctttat
     8041 tggggaggag aaaaatattt tatacaccaa agtgcagcgc gatagtagct ttgtaagggt
     8101 tcctattcaa aaaagcatga ttgttgagaa gaatgctgat gaaaaggctg gcgaggcagc
     8161 cgacctcatt ttcaacctac gcaagcgaag gctggagttc cttactactg atgttgatca
     8221 gcccttggag ggcaatgctg ttcatgtaat tctcgatgaa atttataggc tcgaaaacga
     8281 atacctttcg ttgttcttag gtaaaacagc tgttgatacg atttccaaaa cctatctttt
     8341 taccccctca ggagaaaata atgaaaaggc tatcgccttt cgattttccc ctacaaaagg
     8401 gctgcttgcc caaaatgact tgtcgggtag tcccatgctt gttgaggttg aacccgaacc
     8461 ggttcccgat tcctccgaac ctcttttggc aaccatcggt cgtcttgccg aaaagcaaaa
     8521 gctggcacaa attttttacc gtattccggt tgtctcaacc gtaaaaattt ccgacggtaa
     8581 gtcggaaatt gcttcccgcc gcatacctat ttaccagcta ggacctttgg ttaaaattcc
     8641 tgcagagttt tattaatcca ggtaagctaa acttattcac cgctcgttag cttgcttaca
     8701 aactttgagg caaactgttc ccatggttgg gtcttgtagg catccgacat gatgtaaacc
     8761 ataataggct caatttgcga tggggtaagg tatcccgaaa ggaaggttat taagtttcca
     8821 ttcccatcca taaaagcaat tgaagggtag gcaagcttgc cctgcagtag ggcaatagca
     8881 aaatcgtggg ttgaccgttg cccctctcct tggtttttat atacttgccc cttgtaggta
     8941 atttcattct ttccctcagc attgaactta acggcataaa aatccttggt taggtagctt
     9001 gcaatcagcg ggttgttaaa ggtattgctg tccattttct tacaccagcc gcaccagtca
     9061 gtgtaaacat cgataaatat tttacgcggc tcgctggtgt tcagctttaa ggcttcttca
     9121 agtgtgtacc acttaacctt gctactctcc tgtgctatgg taactccagc tgttaatgtt
     9181 agctgggcta acagtaatgc aaatcttctc atacatttca aatgtttacc acacaaaggt
     9241 aagtaaattg atgactatgg aatttgccct aaggctaatt tttcgtaaaa tcggcacatc
     9301 aaaagcccaa aatataggtg ttcctcccgt ttttttaacg atgcacaaaa gttaaggggt
     9361 aaagtacctt gtaaccagaa acctaaaaat tcccctgatt aaatttttta cctacctcaa
     9421 atcataaggc taaacaaaaa aatgcattct tccgtttggc tttatttccg attttttccc
     9481 aattcatttg cacctggagc ataatggtat tttcgggata ttaaaattct tgctatcttt
     9541 aaaaattcaa aaaacatgtt tacctaacgt atattgctat gaataaggat atttttaaac
     9601 tcgagcctca aaggctatgg aagcactttt acagcttgac acaaatcccc cgcccatcca
     9661 aaaaggaaaa ggctgctgtg gattttatga ttaatttcgg gagaaacctc ggactcgaaa
     9721 caatcaccga tgaggttgga aatgttatca tacgcaagcc tgccactccg ggtatggaga
     9781 atcggaagac tgtgattttg caagggcacc tcgatatggt tccccagaag aacagcgata
     9841 aaaagcacga ttttgagaaa gacccaattg aggcttatgt tgatggagaa tgggttaccg
     9901 caaacggtac aactcttggc gccgataatg gcattggtgt tgctgcagca atggcggtgc
     9961 ttgaagcaaa agacatagct catggccctg ttgaggctct tttcactatt gatgaggaaa
    10021 ctggcatgac cggtgccttt ggcattaaac ccggacttct taagggtgat attcttttga
    10081 atttagattc agaagatgag ggagaactct acgttggttg tgccggtggt cttgatgcaa
    10141 atatccggtt tagctttact cctcagcctg taccttccga tatggttgcc tacaagcttg
    10201 ccgttcttgg acttaagggt ggccattcag ggcttgaaat caaccttggt aggggtaatt
    10261 ccataaagct actattccga ttcctttacc gtgcctgcaa aacactaggc gttcgcattg
    10321 gcactattga tggtggaagc cttagaaacg ctatacctcg tgaatcattc gctgttgtta
    10381 cagttccaag taagaatgca accaattttg aaaagttggt tcgcgagtac gaacaaatct
    10441 acaaaaacga attttcccta acggagccca acctctcatt tacctgtaaa accgattctt
    10501 tacccaaatt cttagttgat gaaaactccc aatcacgggc tattcgcgca tttttcagct
    10561 gccctaatgg tgtggttcgc atgagcgatg ctgttcccgg acttgttgaa acttcgaaca
    10621 acctggctag agtttatacc gaaggaaacc agattgttgg acaatgctta atgcgtagct
    10681 ctgttgactc ggcaaaggag gcattagctg ataaaatggc tgcaatcttt gagttagccg
    10741 atgcagctat tttattcgaa ggcggctacc ccgggtggaa acctaatcca aattccccaa
    10801 tccttactgt aatgcagcaa gtttataaga ataaattcgg aaccactcct gaggtaaagg
    10861 caatccatgc tggtctggaa tgcggcttgc taggtggggt ttatcctaac ctggacatga
    10921 tatcgtttgg accaacaatt cgccaccccc attcacccga cgaaaaggtg aatatcgcta
    10981 cagttggaaa gttctgggaa tttctagttg aagttcttaa aaatgttcct ttaaagggtt
    11041 aaatcttcct aaattcaagt aattaatgca actttttaag ggagtcaggg aagtaaaaaa
    11101 acaaactttt tcaagaaaga ggagaaaaaa gcagcttttc cccccttgtc ctgatggagt
    11161 aaatgcctta ctttgccctc tcccctcaaa aagttacaaa gatgaagcac ttaaccgtag
    11221 agcaaaggta caccatt
//
LOCUS       DAOPHX010000086        11028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_7209_length_11028_cov_5.949512, whole genome shotgun sequence.
ACCESSION   DAOPHX010000086 DAOPHX010000000
VERSION     DAOPHX010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 11028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11028
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7209_length_11028_cov_5.949512"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1202)
                     /locus_tag="PK502_07370"
     CDS             complement(177..1202)
                     /locus_tag="PK502_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPH00673.1"
                     /translation="MQPAEVIGIKNLVVGYGEGAMSDFTYPPINANAYKGEMVALVGR
                     NGAGKSTLLRTISRLQPMMNGEITVNGATLESIGRSEFSQFISFIPAEPVRLPNTTVS
                     DFITLARYPYHGWFDGLSHTDTTMVGKALKAVNLFDYRNRFLDRLSDGERQRVMVAFA
                     IAQDTPVILMDEPTAFLDLPSKFEIVRLLREQTLLGKTVIISTHDLQTAFGFVDTIWL
                     MLREGIRIGSPEDLVLTGAIDSLMDGTLVKFDMSSGQFKYTNSKTLSAYVSGSKRSLV
                     KWTAHALERLGYHVSSIVLDTTSLQVEVMETEGKIQWCLENHKHKPTFDSLRNLCLFI
                     KLEMNAG"
     gene            complement(1189..2256)
                     /locus_tag="PK502_07375"
     CDS             complement(1189..2256)
                     /locus_tag="PK502_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPH00674.1"
                     /translation="MERSSKNYGKSKERKPTGKVGMMAVLLAVLVFLLVFIDLLLGSV
                     RIPMSDVLKFIFTGSAGSEVSQNILFNFRLPRVLAAVLSGSALAVSGLLMQTIFRNPL
                     ADPSVLGISAGASLGVALVVMGSSALGLSFLGNQFSMVLAALAGSMLVLMLVMAVSVR
                     VRNVIVILILGMMFGFAASAVTSILQYFSSEVSLKSFVIWTMGSLSSISGNQLWIFAL
                     LVFLGLVLAMLVARPLNVLGVGENYAQLLGYNVKAIRMVVFISTSLLAGSTTAFVGPV
                     VFVGIAVPHVVRMAVKTSNHHVLLPTTLLAGAAFMLLIDTIAQLPGAGIVLPVNTVAA
                     LMGIPVVIYVIFKGSHFHAAS"
     gene            complement(2309..3451)
                     /locus_tag="PK502_07380"
     CDS             complement(2309..3451)
                     /locus_tag="PK502_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPH00675.1"
                     /translation="MKAIPIYLSLLLALFSCGNRAESRGGSILEMNANYPPKYAEGFT
                     IFNDSTGTKIRVFNPWQGASNVSLEYKLVPRGDISKFSSNPGAIPVPLERVVCMSSSH
                     LAFIEALGMGQRIVGVSGAGFAFSSALRAMVDEGKIFEVGYDQGINYEQIARLNPDVI
                     LCYGIGSESMAYLKKFSEMGMRIMFIGDYLESDPLGKAEWIKVLGCLFNCYGKADSIF
                     SSIESSYLKTKKQVERCSSKPTIFMNMPFRGVWYFPGNDSYMVKLVHDAGGSYVFENL
                     EGSRSFPLGIEKAFEAGMGSDIWLNPGSASSLTDITSFDTRLAQLPPVVSGKVYNNNL
                     RMSRGGGNDFWESGTIHPELILHDLISIFHPEIANDSLLVYYQKLE"
     gene            3665..5890
                     /locus_tag="PK502_07385"
     CDS             3665..5890
                     /locus_tag="PK502_07385"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPH00676.1"
                     /translation="MTRRKYIINWLIVVFDIWLITNSFPIYATSTANPDDSRLQQFSF
                     NDTIARIDLRISEFEKNGNRAELAKALTEKAGIYFRVARYDDALMTANQALLVANEEG
                     REQLLEPIYHLLSLIHIENELVFEAAEYLYAGYQKSLDMKDTANIGWYLVNLSEVEEY
                     LGRVSNAMEINLTAIDFFKNTSDSLSLAKIYRAQGVAHAELKNFLTAEDYFKKAFRIF
                     NSCGDSLNMGLVLMDQASLNLKELNLYEAQSLIGQAMKLLEKQSQKFYLRSRCIYGEL
                     LVQRRSFTEAIEMLKETLMIQLSINDLYGYTATLSALGRAYLGSGNRQMAIDSYTRCA
                     KMADKANLINFKRDSYRNLASILGSEGNSSKAYYYLSSYVTLTDSLFNLQKISEANRL
                     ENQAMLRMKEKEIAQQKEQIILNSSQLRQESVKQKFLYIIIILSFGVTAAVVYSYRQK
                     KTANALLTEQNNRIEKQRDMLEFRSREIMDSLNYAQRIQRAILHSSLQPEDFFPDSFL
                     IFIPKEVVSGDFYWFKAVDNLKLFAIADCTGHGAPGAFMSIIGMFGLNQIITEYGQTH
                     PGDVLNQLNELFYHSFEQREGAEIFDGMDIGFCEYNTTTKELKFAGANIYLYILRKSS
                     LPAPSSITLHKTTDYTLYQVKCNRQSIGYMAEKIEFSTHSITLMADDTIYLFTDGFVD
                     QFGGPQGKKFRYNELRALLCEMATIPMSEQKSRLLEIFTEWKGGYVQVDDVTMLGVRV
                     T"
     gene            complement(6014..6598)
                     /locus_tag="PK502_07390"
     CDS             complement(6014..6598)
                     /locus_tag="PK502_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPH00677.1"
                     /translation="MTDEVRDMIVESAATFFNRFGFKHTTMENIAESIHKAKGLIYYY
                     FKSKEELFKEVLWNDIRRIKAHLMAIANSEDDTHITLKKFVHARLELLEGATSYHEVI
                     KAQIYHKSHVFLDDIKNDFEDFEKSQLSSIIRRGEQRGDFKVESIPSTVNGILTVLGS
                     LDFSLFLIKKRVELDTINDEISTLIVNSVRREIA"
     gene            complement(6635..7762)
                     /locus_tag="PK502_07395"
     CDS             complement(6635..7762)
                     /locus_tag="PK502_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPH00678.1"
                     /translation="MKVTIKEVTDKRLLKKFIKFPNKLYKGNRYYVPQFIASEFSTLS
                     KEKNPAFEFCEARYWLAYNEDNAIVGRIAGIINNRYNQKTATKYVRFGWLDFVEDADV
                     LRALFDTVENWAKANGAQYLHGPLGFSEFDASGILIEGFNELPTAYGKYNYPYYQSMV
                     EQLGFEKEVDWVEYNIKVPDSVPEKYSKMADIIEERNQLHRLKFSSKRELLAYTDEIF
                     QLLNREYSSIHGFAELTQGQIDDLKKQFVPLIQLKFISVVKNAQGEVVGFGICLPSLS
                     KAIQKSKGRMFPFGFIYLIKALKYNDTLDTLLIAIHGDYKNKGVNALIFNDIGKAIIE
                     SGITNIETTRELEDNFMVQNLWNKLEYRQHKRARCYIKRLS"
     gene            complement(7801..8598)
                     /locus_tag="PK502_07400"
     CDS             complement(7801..8598)
                     /locus_tag="PK502_07400"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPH00679.1"
                     /translation="MKVVLITGVSSGFGRAIVLRLANKEFKVYGTVRRKVDPIPGVSY
                     LNMDVTSRESVEKAVGEVYGKEGRIDVLISNAGMGIAGPLEFTSIDDVQHQMDVNFLG
                     MVRVVQAVLPIMRNQKGGRIICISSIAGLIGLPFQGVYSASKFAIEGYCLALRTEVRP
                     HNVEVVLVNPGDFSTSFTRNRSVVDSSEAKEAYPNYIKTMKVVEQEENRGLSPEFLAK
                     KIEKIINRKKCPTRCVVATPVQRASVFIKRILPEGLFYRIIQKYYMS"
     gene            complement(8610..9818)
                     /locus_tag="PK502_07405"
     CDS             complement(8610..9818)
                     /locus_tag="PK502_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HPH00680.1"
                     /translation="MGKLQERYSQYTLPQQIKAEGIYPYFRAIESEQSTVVTMKGKQV
                     LMFGSNSYLGLANHPRIKEAAKKAIDKYGTSCSGSRFLNGTLDIHLELEEKLAKLVGK
                     ESAICYSTGFQVNLGVVSALCSRGDYLLLDCLDHASIIEGSRLSFSKILKFDHNDMSS
                     LEAKLKLCEPDRIKLIVVDGIFSMEGDIANLPVIVQLAEKYDASIMVDDAHSLGVIGD
                     GGRGTASHVNLTPKVDLIMGTFSKSFASLGGFIAGDESVINYLRHNSRSLIFSASMTP
                     ASVASVSTAIDIMLEEPERMKHLWDLTRYAHEAFKSHGIYIGKSETPIIPLYVRDDIK
                     TLQLTKMLLDEGIFINPVITPAVPKEDSMIRFSLMATHTFEQLDIAIDKIVGSFKKLG
                     LLTTSTVKNP"
     gene            complement(9830..10291)
                     /locus_tag="PK502_07410"
     CDS             complement(9830..10291)
                     /locus_tag="PK502_07410"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPH00681.1"
                     /translation="MPKYFKFIASRLIGTIVDTLVLWLLTSFIFSSYVGAYIIAPTIS
                     FEFAVFSNFLFSYFWIWSKRVAVKNNRSFFSHFLSFNISSFLGFIVKMGFLLLFSKLL
                     KWSVVYCNFLALLISGVVNFGLSELVVFKKSPVVDSVDEKILVRRNKSGKF"
     gene            complement(10298..11002)
                     /locus_tag="PK502_07415"
     CDS             complement(10298..11002)
                     /locus_tag="PK502_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPH00682.1"
                     /translation="MNGKTEVRIQTSILNKMEKRILVAMANRMPSWITSDNLTVVGVI
                     GALTTGVGYILSSWNLNFLWLASFGLILNWFGDSLDGTLARVRNAQRPIYGFYIDHNI
                     DGLTITIMCIGAGLSPMIKFSTAMLILVGYLLLSIFTYINAYLKGEFRISYGNLGPTE
                     FRLIVIIINTMFIYFPFKDMYLHIMKYEVGVYDAIGFGIAILLFFIFLISFFVFLRKY
                     KQVDPPRWVDNHNNSN"
BASE COUNT         3137 a         2649 c         2328 g         2914 t
ORIGIN      
        1 tatatttaac cgaagaactt cttcgtattg gaataaatta tatgtcccat tgctgtcgca
       61 cggttgcctt cgccgagctc cccgccgggc ggcgggtcgg ttcgtgctgt aacgagaagt
      121 taactttttg tttattcggt tcccggttca cttgtggcat ttccctgctc cacgccctac
      181 ccagcattca tttccagctt tataaacaag caaaggtttc ttagggaatc gaaggtgggc
      241 ttatgtttat ggttttctaa acaccactga attttccctt cggtttccat cacctcaacc
      301 tgcagggaag ttgtatctaa aacaatactt gaaacatggt agccaagtcg ttccaacgca
      361 tgggcagtcc attttaccaa gcttcgttta cttcccgaga cgtaagcgct aagggttttg
      421 gagttggtgt acttaaactg tccggagctc atgtcgaact taaccagggt tccatccatc
      481 aggctgtcta tagcaccggt tagcactaag tcctcaggcg aaccaattct aatgccctcg
      541 cgaagcataa gccagattgt gtcaacaaag ccaaaggcag tttgaagatc gtgggttgag
      601 atgatgaccg ttttccccag cagggtttgc tcgcggagta accttacaat ttcaaacttg
      661 ctcggcaaat cgaggaatgc cgttggctcg tccatcagga ttacaggtgt atcctgggca
      721 atggcaaagg caaccatcac ccgctgcctt tcgccatcac tcagcctatc gaggaagcga
      781 ttccggtagt caaacaggtt tacggcctta agggctttgc ccaccatagt cgtgtcggtg
      841 tggctgagcc catcaaacca gccatggtag gggtaacggg caagtgtaat aaaatcggag
      901 accgtggtat taggcaaccg aacaggttct gccggaataa agctaataaa ctgtgaaaat
      961 tcagagcgtc caatggattc caaagtagcg ccatttacgg taatttcgcc attcatcatg
     1021 ggttgaagcc gtgaaatggt gcgcagcagg gtgctttttc ccgctccgtt ccgtcccacc
     1081 agcgccacca tttctccctt ataagcgttg gcattgatag gtggataggt aaaatcggac
     1141 atagccccct caccataccc aactaccagg tttttaattc ctattacctc agctggctgc
     1201 atggaagtgg ctacctttaa agattacgta aattacaaca ggaatgccca ttaacgctgc
     1261 cacggtattc actggaagca caattccggc gccgggcagc tgggcaatag tatcaatcag
     1321 gagcataaat gcagcacctg ctaatagcgt ggtgggtaaa agcacatgat ggttggaagt
     1381 tttaacggcc attcgcacca catgaggaac agcaatacca acaaaaacaa ccggaccgac
     1441 aaatgcagtg gttgagccgg ccagcaggct ggttgagata aaaactacca ttctgatagc
     1501 tttaacgtta tagcccagca gctgggcgta attttctcca acgcccagca cgttcaatgg
     1561 tcgggcaacc agcatggcca gcacaagccc aaggaaaacc agcagggcga aaatccatag
     1621 ctggtttccg ctaatgctac ttaggctgcc catagtccaa ataacaaacg atttgaggga
     1681 tacctcgctg ctaaaatact gtaaaatgct ggttactgct gaggcagcaa agccaaacat
     1741 cattcccaaa atcagaataa caataacgtt gcgcaccctc accgaaacgg ccattaccag
     1801 catgagcaca agcattgaac ctgccaatgc ggccagcacc atactaaatt gatttcccaa
     1861 aaatgaaagc cctaacgccg atgaacccat caccaccaaa gcaaccccca agctggcacc
     1921 agcactaatt cccagcaccg aagggtcggc caagggattt cgaaatatgg tttgcatcag
     1981 caagccgctt accgccagag ccgaacccga cagcacagct gccagaactc tgggcaacct
     2041 gaagttaaac agtatgtttt ggcttacctc gctgcctgcg cttccggtaa atataaactt
     2101 tagcacgtca ctcatgggaa ttctaaccga ccccaacagt aaatcaataa aaaccagtaa
     2161 aaaaaccagc acagcaagca aaactgccat catgcctacc ttccctgtag gctttctttc
     2221 cttactttta ccgtagtttt tactgctccg ctccatcact attactcagc ttacgccaaa
     2281 atcaatggtt atctcgttag atgttaagct actccaactt ttggtagtaa accaaaagtg
     2341 aatcgttcgc aatttcgggg tggaaaatgg aaataaggtc gtgcaggatc aactcgggat
     2401 gaattgttcc cgattcccaa aagtcgttac cccctccccg gctcatccgg aggttattgt
     2461 tatacacctt gccagaaact actggtggca gctgggctaa gcgcgtatca aacgaggtga
     2521 tatccgtcaa gctgcttgct gaacccgggt ttagccagat atcactgccc attccggcct
     2581 caaatgcttt ttctattccc agtgggaagc ttcggctacc ctccaggttc tcaaaaacat
     2641 agctgcctcc ggcatcatgc accagcttca ccatgtagct gtcattaccc gggaaatacc
     2701 acacaccccg aaagggcatg ttcataaaaa tggttggttt tgagctgcaa cgctcaacct
     2761 gctttttggt tttcaggtag ctactttcaa tgctactaaa aatcgaatcg gctttgccgt
     2821 agcaattaaa aagacagcct aaaaccttaa tccattcagc cttaccaagc ggatcggact
     2881 ccaggtagtc gcctataaac ataatgcgca tccccatttc gctaaacttt ttaaggtatg
     2941 ccattgattc ggaaccaatt ccgtagcaaa gaattacatc tggattcaac cgggcaatct
     3001 gctcgtagtt tatcccctga tcatagccaa cctcgaatat ttttccctca tcaaccattg
     3061 ccctcagcgc ggagctgaaa gcaaaccccg caccggaaac tcccacaatt cgctgtccca
     3121 ttcccaacgc ctcaataaat gccaaatggc tggacgacat gcaaacaaca cgttccagcg
     3181 gaaccggaat tgcaccaggg ttggagctaa atttactaat atcccccctt ggaaccagct
     3241 tatactccag gctaacattg ctggcacctt gccaggggtt gaaaacccgt attttagtgc
     3301 cggtgctatc gttgaaaata gtaaaaccct cggcatactt tggcggataa ttggcgttca
     3361 tctccaagat tgagccacct ctgctctccg cacgattgcc acatgaaaac agggcgagca
     3421 acagggatag gtatatgggt atggccttca ttttttccgc taaagtagct gaatatgccc
     3481 atatccacca accggttgtg aaaaattaca tccagaaagt aggcagcagc tcaaaaaagc
     3541 tgaatgataa gctaaagcgc tgccatcatg ttgaaatggc acaaaaaaga attctgggta
     3601 agagtaagaa tattcctgat aagaagctac cttcacacat tgaaaatttg atttcaaatt
     3661 taggatgact aggcggaaat acataataaa ttggcttata gttgttttcg atatatggtt
     3721 gattaccaac tcatttccca tttatgctac gtcaaccgcc aaccctgacg atagccgatt
     3781 gcaacagttt tcttttaatg atactatcgc caggattgat ttgcggataa gcgaattcga
     3841 aaaaaacggt aacagggccg aacttgccaa ggcactaacg gaaaaggcgg gcatctattt
     3901 tagagtagcc cgctatgacg atgcactaat gacggccaac caggccttgc ttgtggccaa
     3961 cgaggaagga cgtgaacaac tccttgaacc aatataccat ctgttatccc ttatccacat
     4021 tgaaaatgag ctggtgtttg aagcagccga atatctttat gctggctacc agaaaagcct
     4081 ggacatgaag gataccgcaa atattggctg gtacttggtt aacctaagtg aagttgagga
     4141 gtacctgggc cgggtttcta acgctatgga gattaacctg actgcaattg atttctttaa
     4201 aaacaccagt gactcgctaa gcctggccaa aatataccgt gctcaaggag tagcacatgc
     4261 cgagctgaaa aacttcctaa ccgccgagga ttactttaaa aaggcctttc gaatattcaa
     4321 cagctgtggc gattcgctta acatggggtt ggttctgatg gatcaggcaa gcctcaacct
     4381 caaagagcta aacctttacg aggctcaaag ccttattggg caggcaatga agctgcttga
     4441 aaagcaaagt caaaaattct acctccgcag caggtgcatc tacggcgagc tgcttgttca
     4501 acgacggagc ttcaccgaag ccattgagat gctgaaggag actctcatga ttcagctctc
     4561 aataaacgac ctttacgggt acaccgccac cctatcggca ctcggaagag catacctggg
     4621 ttcgggtaat cgccaaatgg ctattgacag ctatacccgc tgcgctaaga tggccgacaa
     4681 ggccaacctc attaacttta aaagagactc ataccgcaac cttgcgtcca tacttggctc
     4741 cgaggggaac agcagtaagg catactacta cctatcgagc tacgtaaccc taaccgatag
     4801 ccttttcaac ctacaaaaga taagcgaagc aaaccggttg gagaaccaag cgatgctgag
     4861 aatgaaggag aaggagattg cccagcagaa ggagcaaatc attttgaata gctcgcagct
     4921 aaggcaggaa agcgtgaagc aaaaatttct ttacatcatc attatccttt cgtttggagt
     4981 aacagctgcc gttgtttaca gctatcgaca aaaaaagact gcaaatgccc tgttaaccga
     5041 gcaaaacaac aggattgaaa agcaaaggga tatgctggaa ttcaggtcac gcgaaattat
     5101 ggatagcctc aactatgccc agcgtattca gcgggctata ctccattcat cgcttcaacc
     5161 cgaagatttt tttcccgatt cgttcctgat ttttattcca aaggaggtgg ttagtggcga
     5221 tttttactgg tttaaagcgg ttgataactt gaagctgttt gccattgcag actgtactgg
     5281 acatggagcg ccaggggcat ttatgagtat tattgggatg tttgggctca accagattat
     5341 taccgagtac gggcaaacac atccgggaga cgtgctcaac cagctaaacg agctctttta
     5401 ccactcgttt gaacagcgtg agggtgctga aatctttgac ggcatggaca ttggtttttg
     5461 tgagtacaac acaaccacca aggagctaaa atttgccgga gcaaatattt acctctacat
     5521 cctccgcaaa tcaagcttgc ccgccccttc gtcaatcacc cttcataaaa ctaccgatta
     5581 taccctttac caggttaagt gcaaccgcca gtcgattggt tacatggctg aaaagataga
     5641 attttccacc catagcatta cgttaatggc cgatgacacc atctacctgt tcaccgatgg
     5701 ctttgttgac cagtttggtg gccctcaggg taaaaaattc aggtacaatg agctccgggc
     5761 acttctctgc gaaatggcta ccatacccat gagcgaacaa aagagcaggt tactggagat
     5821 tttcaccgaa tggaagggtg gctacgtgca ggtggatgat gttaccatgc tgggcgttag
     5881 agtaacctaa aaaaggagta cggagattct cgcggatgga gggaagttat agctttaaca
     5941 ttaaattttc tcacggatca agtctcacat cccacagctc aaagctgttc acaacttttt
     6001 tcttgttttt acattaggca atttctcttc taacgctgtt gacaatcaag gtggaaattt
     6061 cgtcatttat ggtatctaac tccacacgct tttttataag aaacaatgaa aagtcgaggc
     6121 tgcctaagac tgtcaaaata ccattgactg ttgaagggat gctttcaact ttaaagtcgc
     6181 cgcgctgctc tcccctacga ataatacttg atagctgact tttctcaaaa tcctcaaagt
     6241 cattttttat atcatctaaa aaaacgtggc ttttatggta aatttgtgcc ttaataacct
     6301 catgatagct ggtagccccc tcaagcaact ctaaacgtgc atgtacaaac ttttttagtg
     6361 taatgtgagt gtcatcctcg gagttggcta ttgccatgag atgcgcttta atgcgcctga
     6421 tatcattcca taaaacctcc ttaaacagct cctccttact tttgaaatag tagtatatca
     6481 agcctttagc cttatgaatg ctttcggcaa tgttttccat ggtggtatgt ttaaaaccaa
     6541 atcggttaaa aaaggtggca gccgattcca caatcatatc ccttacctcg tctgtcatag
     6601 ctattgaaat ccttattggt tatacaaaaa gctgttatga aagccttttt atgtagcatc
     6661 ttgccctctt gtgctgacgg tactcaagct tgttccataa attttgaacc atgaagttat
     6721 cctccagctc gcgggtggtt tcaatgtttg ttatgccgct ttcaataata gcctttccaa
     6781 tgtcgttgaa tataagcgca ttaacgccct tattcttgta atcgccatgt atggctatta
     6841 acagggtatc aagtgtgtcg ttatacttca gtgcttttat gaggtagata aacccgaaag
     6901 ggaacattcg tccttttgat ttctgaatgg cttttgatag ggatgggaga caaatcccaa
     6961 aacctaccac ctccccctgt gcgtttttga ccaccgaaat gaattttagc tggatcagcg
     7021 ggacaaactg cttcttaagg tcatctatct gaccttgagt tagctcggca aagccgtgga
     7081 tgctggagta ctccctgtta agaagttgga atatctcatc ggtataggca agcagctctc
     7141 tcttactcga aaatttgagg cgatgtagct ggttacgctc ttcaatgata tcggccattt
     7201 tggagtactt ttctggaacg gagtcgggca ccttgatgtt gtactccacc cagtcaacct
     7261 ccttctcaaa tcccagctgc tcaaccatgg actggtagta agggtagtta tacttaccat
     7321 aggcagttgg cagctcgttg aacccctcaa ttagaattcc agaagcatca aattctgaga
     7381 atcctagagg gccatgaagg tattgagctc cattcgcctt ggcccaattc tcaaccgtat
     7441 cgaataacgc cctaagcaca tcggcatctt caacaaaatc gagccacccg aatcgtacgt
     7501 attttgttgc cgttttttgg ttatacctat tgtttatgat tccggcaata cgacctacaa
     7561 tggcgttgtc ctcgttatag gccagccagt acctggcttc acaaaattca aaagctggat
     7621 ttttttcttt tgataatgtg ctaaactcgc tggcaatgaa ttggggtacg tagtacctgt
     7681 tgcctttata gagcttatta ggaaacttaa tgaatttctt taacaacctc ttgtctgtaa
     7741 cctccttaat agtaactttc attcaattca ggaattagta ggtaagtaat agccgcacct
     7801 tcaggacata tagtactttt gaataattcg gtaaaacaaa ccctcgggta gtattctttt
     7861 gataaatacg gatgccctct gaaccggtgt agccacaacg caacgggtag gacatttctt
     7921 cctatttata attttctcaa tttttttggc aagaaactcc ggcgaaagcc ctctgttctc
     7981 ctcctgctca actaccttca ttgttttgat ataattgggg taggcttcct ttgcctctga
     8041 tgaatcaaca acgctcctgt tgcgggtaaa gctggtggag aaatcgcccg ggtttacaag
     8101 tacaacctca acgttgtgag gccttacctc cgttcgcagg gcaagacaat acccctctat
     8161 ggcaaatttg ctggccgagt aaacgccctg aaaaggtagc ccaattaaac ccgcaataga
     8221 gctaatgcag attattctac cacctttttg attacgcata attggtagaa cagcctgaac
     8281 tacccttacc attcccaaaa agttcacatc catttgatgt tgcacatcat caatacttgt
     8341 aaactcaagt gggccggcta tacccattcc cgcattgcta atcagcacgt ctatacgccc
     8401 ctcctttcca taaacctccc caacagcctt ttcaaccgat tcccggctcg ttacgtccat
     8461 gttgaggtag ctaacacctg ggattggatc gaccttccgg cgcacggtgc cataaacctt
     8521 aaattcctta ttggcaagcc gcagtacaat agcgcgacca aatccagagg aaacacccgt
     8581 tatgagaaca accttcattt gcttgtaggc tagggatttt tcacggttga ggtggtaagc
     8641 aacccgagct tcttaaatga acccacaatt ttatctatgg caatgtccag ctgttcgaag
     8701 gtatgggtag ccatgagtga gaagcgtatc atggagtctt ccttcggaac agcaggtgta
     8761 ataacagggt tgatgaatat tccctcatct aaaagcattt tagtaagctg aagggtttta
     8821 atatcatcgc gaacgtaaag aggaataatg ggggtttcgc ttttgccaat gtaaatgcca
     8881 tgactcttaa aagcctcatg ggcatatcgg gttaaatccc aaagatgctt cattcgttcc
     8941 ggttcttcga gcattatatc aatggcagtg ctaacgctgg ccaccgaggc aggagtcatg
     9001 ctggcgctaa aaatcagcga gcgggagtta tgcctaaggt agttgataac cgactcgtct
     9061 ccggctataa aacctcctag tgaggcaaag gattttgaaa aagttcccat aatcaggtcc
     9121 accttagggg taaggttgac gtgcgaggcg gttcccctgc ccccatcccc aattacaccc
     9181 agcgaatggg catcgtcaac cattatgctt gcatcgtact tctcggctag ctgaacaatg
     9241 acgggcaagt tggctatatc gccctccatg gagaatattc cgtcaaccac aataagctta
     9301 atccggtcgg gctcacaaag cttaagcttg gcttccagcg agctcatatc attatggtcg
     9361 aacttcagaa ttttggaaaa agacagccgg cttccttcta taatagaggc atggtcgagg
     9421 caatcgagca ggaggtagtc accacggcta caaagagccg aaacaactcc cagattaacc
     9481 tggaagcctg tgctgtaaca gattgccgat tccttaccaa ccagcttagc cagcttttcc
     9541 tcgagctcaa ggtgaatgtc gagcgttcca ttcaggaatc ttgaacccga acagctggtg
     9601 ccgtacttgt ctatcgcctt ctttgccgcc tccttaatcc ggggatggtt ggccaaaccc
     9661 agatagctgt tagagccaaa cattaaaacc tgcttgccct tcatggtaac cacggtgctt
     9721 tgctcggatt caatcgccct aaagtaaggg taaattccct cagcctttat ttgctggggc
     9781 agggtgtact gcgagtaacg ttcttgaagt tttcccatat ccattcaagt taaaacttac
     9841 cagatttatt tctacgaacc aatatctttt cgtccaccga gtccactacg ggggactttt
     9901 taaatacaac cagctccgaa agtccgaagt tgacaactcc ggaaatgagc agtgctaaaa
     9961 aattacagta aaccacactc cacttcaaca gcttgctaaa tagtagcaaa aatcccattt
    10021 taacaataaa tcccagaaaa gaagatatgt tgaatgataa aaagtgacta aaaaaagatc
    10081 tgttgttttt tacagctacg cgcttactcc atatccaaaa gtatgaaaat aaaaaatttg
    10141 aaaaaaccgc aaattcgaat gagattgtag gtgctatgat gtatgctcca acataggagg
    10201 aaaagataaa gctcgtgagc agccacagca ctagcgtatc gacaattgtc ccaattagcc
    10261 ggctggcaat aaatttgaag tacttgggca tcatgggcta attcgaattg ttatggttat
    10321 cgacccaacg gggagggtca acctgtttgt acttccttaa aaagacaaag aagctgataa
    10381 gaaaaataaa gaaaagcaga atggcaattc caaacccaat ggcatcgtaa actccaacct
    10441 catacttcat aatgtgaagg tacatatctt taaatggaaa gtatataaac attgtgttga
    10501 taatgattac gataagcctg aattcggtgg gtcccaggtt gccgtatgat attctaaact
    10561 caccttttag gtaggcatta atataggtga aaattgacag aagcaggtaa ccaaccagta
    10621 ttagcatggc ggtagaaaac tttatcatgg gcgaaaggcc ggcgccaata cacataatgg
    10681 taatggtgag cccatcaatg ttatgatcta tataaaatcc gtaaattggc ctttgagcat
    10741 ttcttaccct tgccaatgtt ccatcaaggc tatcgccaaa ccaatttaga ataagcccaa
    10801 acgaggcaag ccataaaaaa ttaaggttcc agctggaaag tatgtatcct accccggtcg
    10861 ttaatgcgcc gatcacaccc actacggtga ggttatcaga ggttatccag ctgggcattc
    10921 gattggccat cgccaccaaa atcctttttt ccatcttatt cagaatggaa gtctgtattc
    10981 ttacttcggt tttaccattc attttaacat atttttatta caatttcc
//
LOCUS       DAOPHX010000087        10994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_7250_length_10994_cov_6.299205, whole genome shotgun sequence.
ACCESSION   DAOPHX010000087 DAOPHX010000000
VERSION     DAOPHX010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 10994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10994
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7250_length_10994_cov_6.299205"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..984)
                     /locus_tag="PK502_07420"
     CDS             complement(34..984)
                     /locus_tag="PK502_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329363.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPH00683.1"
                     /translation="MSDVYPWGDNRRFNTYSGYFKREFGGRVQKLAIDAGFSCPNRDG
                     TVATGGCTYCNNEAFNPSYCHPTKSITQQIEEGIEFHKVRYRRAKRFLAYFQAYSNTH
                     APIEHLRMLYEEALAFPGVIGLVIGTRPDCVDEEKLNYIGQLAAKHYVTLEYGVESCY
                     NRTLEAINRGHTFEQSVWAIEETHRRGIKVGAHIILGLPGETRNEMLAEASILSSLPI
                     HTVKFHQLQVIRGTVMAKRYELDPGAFQLFGMEEYLNFMVDVVERLNPNMVVERITGE
                     APPRFLATNGWGSYRTDQLMVMFEKLLEQRNTWQGRLYKG"
     gene            1430..3397
                     /gene="rpsA"
                     /locus_tag="PK502_07425"
     CDS             1430..3397
                     /gene="rpsA"
                     /locus_tag="PK502_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPH00684.1"
                     /translation="MAKEATELEKPTPVAGENVNTVNLENFDWDLYAREGAYTPEQRK
                     ELEDIYDKTLSTVAENEVVEGTVVAITKREVFVNVGYKSEGVLSISEFRYNPDLKVGD
                     KIEVYVESSEDKRGQLVLSHKNARVLRSWDRVNAALEKDEIIKGYIKCRTKGGMIVDV
                     FGIEAFLPGSQIDVKPIRDYDIYVGKTMEFKVVKINQEYKNVVVSHKALIEAELEQQK
                     KEIISKLEKGQVLEGTVKNITSYGVFIDLGGVDGLIHITDLSWGRVNHPEEVVQLDQK
                     LNVVILDFDDAKKRIALGLKQLTPHPWDSLDTNLKVGDVVKGKVVVIADYGAFVEIAP
                     GVEGLIHVSEMSWSQHLRSAQEFLKVGDIVEAQILTLDREERKMSLGMKQLKPDPWDK
                     IDEKYPLGSKHTAKVRNFTSFGIFVEIEEGVDGLIHISDLSWTKKIKHPAEFTSVGAE
                     IEVVVLEIDKENRRLSLGHKQLEENPWDVFETIFTVDSIHEGTVIDVFDKGAVVALPY
                     GVEGFVTPKHLVKEDGSSAKVDEKLPFRVLEFNKAAKRIILSHSRTYEEDKKAEEKAD
                     KKSKTSSTQKAVKKLNANIEKTTLGDISELAALKTELEENSKKEAKAKKETKPKKETK
                     TAKAAASEDENVKVDEPKADAEAKDEPKVDE"
     gene            3430..3795
                     /locus_tag="PK502_07430"
     CDS             3430..3795
                     /locus_tag="PK502_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPH00685.1"
                     /translation="MEFKIEKFDKFTLVEVLEEKLDTNIAPGLKSELVLISGNGERNL
                     ILDLSTCRYCDSSGLSAILVANRLCKNAGGTFVLTGLTDAVERLITISQLDTVLNIAY
                     SMEEAVQIIENAEMESSKK"
     gene            3792..4718
                     /locus_tag="PK502_07435"
     CDS             3792..4718
                     /locus_tag="PK502_07435"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HPH00686.1"
                     /translation="MTFSVTILGSSSAIPTPTRFPSAHVLNVHERLFLVDCAEGTQMQ
                     LRRFHIRMNRINHIFITHLHGDHVLGLFGLISTFNLLGRKQPLHLFAPKELESVLNFN
                     VGVFTDKLSFPVIFHPVDPNMSQLVFEDRHVSVTSLPLRHKVPTVGYLFKETPSLPNI
                     RKDCIQQYSLGLADIVKIKNGADYIAPDGKIIPNAELTFHARLPRSFAYCSDTLYSER
                     LVGLIRGVDLLYHEATFLDADVDLARQTGHSTALQAATAAVGAHVGKLVIGHFSSRYK
                     NISQHLQEARNIFPETYAAEDGQVYAIPEKFG"
     gene            4822..6351
                     /gene="guaA"
                     /locus_tag="PK502_07440"
     CDS             4822..6351
                     /gene="guaA"
                     /locus_tag="PK502_07440"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPH00687.1"
                     /translation="MYEKIVILDFGSQYTQLIARRIRELNVYCEIYPYNQIPVLDGTI
                     KGVILSGSPFSVNDNASPKPDLSGIRGKLPILGICYGAQLLAASSGGQVMPSKSREYG
                     RATLEVMDVSSSLFKDFPIQSQVWMSHGDTITALPRNFLITASTTDIQVGAFQVTNEQ
                     TYGIQFHPEVYHTTEGITLLRNFVVDICGCSQTWTPASFIEETTRNLRETLGSDKVVL
                     GLSGGVDSSVAALLLHRAIGKNLTCIFVDNGLLRKNEYQQVLDSFGSMGLNIVGVDAS
                     QQFYNELKGVTDPESKRKIIGRNFIEVFEREAQKIQGVSWLAQGTIYPDVIESVSVNG
                     PSAKIKSHHNVGGLPNQMNLKVVEPLRLLFKDEVRRVGKELGLSAAILNRHPFPGPGL
                     GIRVIGEVTPQRVKLLQEADNIYIKALRESELYDKIWQAGAILLPVQSVGVMGDERTY
                     ENVVALRAVTSTDGMTADWYPFPHNFLSRVSNEIINKVRGINRVVYDISSKPPATIEW
                     E"
     gene            6416..8011
                     /locus_tag="PK502_07445"
     CDS             6416..8011
                     /locus_tag="PK502_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPH00688.1"
                     /translation="MTKKLLYLCAFALIMPFGALSQNSSSFGAYKFVRFIQYLSGDYV
                     DTINVNKLVDNAIVEILSQLDPHSIYISKEDVQAMNEPLEGNFEGIGVEFNILNDTLM
                     VVNPISGGPSERVGILAGDRIIAIDGKSITNINLKITDVHKLLRGPKGTKVTVLIHRK
                     SLSTPVEFTITRDRIPIYSIDASYMAEQGIGYIKINRFAATTEQEFVEAMNKLNQQKM
                     NDLIIDLRNNGGGLLNSAYDLANYFFDPGKLIVYTEGRNSPRTNYFSTSKNKVFRGRV
                     VVLIDEGSASASEILAGAFQDWDRGIIVGRRSFGKGLVQNQLPLPDGSMIRLTVARYF
                     TPTGRAIQRQYKFGDLKDYYSDMTQRYDRGELFNKDSIHFADSLTYKTLIRHKTVYGG
                     GGIMPDVFIPLDTNYYSDYYGKLVRSGVINQFSLTWVDTHRNELLKEYPKFDKFKKNF
                     QVTEKMISELVALAESSGIPSDEKGLAQSANELKIQLKGLVAQSIFSTSHYFEVTNET
                     NETYQKALEIFHSWNDYKHLVQE"
     gene            8082..9659
                     /locus_tag="PK502_07450"
     CDS             8082..9659
                     /locus_tag="PK502_07450"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPH00689.1"
                     /translation="MEALDEFKELGLSEKALAAIKAKGFEQPSPIQKITIPVLLKETT
                     DVIAQAQTGTGKTAAFGLPIIEMFEGQPAGIKALILVPTRELALQVCDEIISLAAKRI
                     EVVAVYGGQSISEQQRRIRRGVDVVVGTPGRILDLIARKDLNFQNLKIFVLDEADEML
                     NMGFIEDIEAIMEHTPKEKRTLLFSATMPDRIVKLAKKYMKSAVKLEVDKAQPTTGLT
                     DQIYFEVRDSDKFDALTRIIDIEPEFYGLIFCRTRNDVDSLTSKLIERGYAAEGLHGE
                     ITQSQREKTLLKFRRRLLNILVATDVAARGIDIIDLTHVINYNMPQDADSYIHRIGRT
                     GRAGKQGTAITFISHDEYRKLNYVQKATKSNIRKEILPAAEDIVAVKKEKIKDELHEI
                     IKQGSYNDYLGMAEDILETNSPEVALAALLRLAYKSQLDVSNYSEARTFRVDTKGTTR
                     LFIGLGRNDGMTPKKISSFIRQEVQIPDNKIDDIVVRDDFSFITVPFVDAEKILKAFA
                     NQETDGKPLITKAKPKK"
     gene            9674..10864
                     /locus_tag="PK502_07455"
     CDS             9674..10864
                     /locus_tag="PK502_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HPH00690.1"
                     /translation="MVEKFEMIAKTLQGLEGVLADELTELGAEDVKIENRAVSFRGTT
                     ETLYKANFWLRTAIRVLKPVYSFSARTIEEFYGNARKFDWTSLMDLSQKFAVDSVVNS
                     EVFPHSRYIALKFKDAVVDQFRDRYGKRPFVDTKNPQIQFHVHISGDSCTILLDSSGE
                     SLHRRGYRTAQDIAPINEVLAAGLIKLSGWNMDSTFIDPMCGSGTIVIEAALMAYGIA
                     PGVFRRQFGFENWLDFDEKLLQSIYNDDSMEREFTHPIIGRDISRQTVESALLNVKHA
                     GLQKKVDIEVGSIFDFQPPADSQGIVVTNPPYGERLRREQIETFYKQLGDCFKQKYEG
                     YNVWMISSNMDALKNFGLRPSKRYTLYNGALECKFQRYSMFAGSVKAKYQGKGERIKS
                     KDRE"
BASE COUNT         3030 a         2472 c         2595 g         2897 t
ORIGIN      
        1 ttttgaatct tgaatttttt tatcctttac cctttatcct ttatacaacc ttccctgcca
       61 ggtattcctc tgttcgagta gcttctcgaa catgaccata agctgatcgg ttctgtagct
      121 accccaacca ttagtggcca ggaatcgggg cggagcttct cccgttatcc gctccaccac
      181 catgttgggg tttaaccttt ctaccacgtc aaccataaag ttgaggtact cctccatccc
      241 aaaaagttgg aatgccccag gatctaattc ataccgtttt gccatgactg tccccctgat
      301 tacctgaagt tgatggaact tcacggtgtg aataggaagg gaggatagaa tgctggcttc
      361 agccagcatt tcgttgcgcg tctcgcctgg tagcccaaga attatatgag caccaacctt
      421 tattccccgc ctatgcgttt cctcgatagc ccaaaccgat tgctcgaagg tatgccccct
      481 gttaatcgcc tcaagcgttc ggttgtagca cgattccaca ccgtactcaa gagttacgta
      541 atgcttagct gcaagctgtc cgatgtagtt gagtttttct tcatcaacac aatcggggcg
      601 tgttccaata accagcccta taaccccggg aaaggccagc gcttcttcgt aaagcatgcg
      661 aaggtgctct attggtgcat gggtattgga gtaggcttga aagtatgcca gaaatctttt
      721 tgcccgacga tagcgaacct tatgaaactc aatcccttcc tcaatttgct gggtaattga
      781 ttttgtgggg tggcagtagc tggggttaaa cgcctcatta ttacagtagg tgcatccacc
      841 ggttgcaact gtaccgtcac ggttcggaca cgaaaaaccg gcatcaatgg ccaacttttg
      901 aactctacca ccaaactccc gtttaaagta acccgagtag gtgttgaatc ggcggttatc
      961 gccccatggg taaacgtcgc tcatctacgt gcacgcttgt ttttttggtg taagtgtact
     1021 ggtagagcct ttctgatatt ggcctatagc aaaatcagct atctcagatg gctgtttatg
     1081 atgtgcaaag gtagcgattt caatgaactg tatattcgta aaagttggta ttgcattttt
     1141 gcttgggtag ctataacttt tacttcttga tcttgttgag cacttcaaaa ttctgatttg
     1201 tggttctttg ttggcaatat ctggctacct atgcaggtaa tttgtttttt agctgttaaa
     1261 ataatttttt atctttatga atttcagcta tattgcagat actttttaga aaaaaatggt
     1321 attttttcct ataaatgtta aagttatata tttttgcagt ttgattaatt cccacacata
     1381 atgtctaact aattaattgt aaatcgttta aatgacaaat caggaagaaa tggccaaaga
     1441 ggctacagaa ttagaaaaac ctacccctgt ggcaggagag aatgttaaca ctgtgaattt
     1501 agaaaatttt gactgggatt tgtacgccag ggaaggggcc tacacccccg agcaacgaaa
     1561 ggagctggag gatatttatg acaaaactct atcaacagtt gctgaaaatg aggtagttga
     1621 gggaacagtc gtagcaatta ccaagcgtga ggtatttgtt aatgtcggct acaagtcgga
     1681 gggagtgctg agtatcagcg agtttcgtta taatcccgat ttaaaggtag gcgataagat
     1741 tgaagtttat gttgaaagtt cagaggacaa acgcggacag ctggttcttt cgcataaaaa
     1801 tgcccgtgtt ctacgctcat gggatcgtgt taatgcagcc ctggagaagg atgagataat
     1861 taagggatat ataaaatgca gaactaaggg cggtatgata gtggatgttt tcggtattga
     1921 ggcattcctg cccggctctc aaattgatgt gaagccaata cgcgactacg acatctacgt
     1981 gggaaaaacc atggagttca aggttgttaa aatcaaccag gagtataaaa atgttgtggt
     2041 gtcgcataag gcgcttatcg aggccgaact cgaacagcaa aagaaggaaa tcatctccaa
     2101 actcgaaaaa ggtcaggtgc ttgagggcac cgttaaaaat attaccagct acggcgtatt
     2161 cattgatttg ggaggcgttg atgggctcat ccatattacc gacctttcct ggggccgggt
     2221 caaccacccc gaggaggttg ttcagctcga ccaaaaactt aacgtggtaa tcctcgattt
     2281 tgatgatgcc aaaaagcgta ttgcccttgg gctgaagcag cttacacccc atccttggga
     2341 ttcgctcgat actaacctca aggttggcga tgtggttaaa ggtaaggttg tggttattgc
     2401 cgattacggg gcttttgttg aaattgcccc cggcgttgaa ggccttattc atgtttccga
     2461 aatgtcgtgg agccagcacc tgcgtagcgc tcaggagttt ctcaaggtgg gcgatatagt
     2521 tgaagctcaa atactcaccc tcgatcgtga ggagcgcaag atgtcgctgg gaatgaagca
     2581 gcttaaaccc gacccatggg ataagattga cgaaaagtat cctttgggtt ctaagcatac
     2641 cgcaaaggtt aggaatttta cctcctttgg gatttttgta gaaattgagg aaggggttga
     2701 tgggcttatc catatctccg acctaagctg gacaaagaaa attaaacacc ctgccgagtt
     2761 tacctcggtt ggtgcagaaa tagaggtagt tgttcttgaa attgacaagg aaaaccgtcg
     2821 cctcagcttg ggccacaagc agctggagga aaacccatgg gatgttttcg aaaccatttt
     2881 caccgttgac tccatccatg agggcaccgt catcgatgta ttcgacaagg gcgccgttgt
     2941 ggccttaccc tatggggttg aaggatttgt tacccctaag catctggtta aggaagacgg
     3001 ctcctcagct aaagttgacg agaagcttcc cttcagggtg ctcgaattca acaaggcagc
     3061 taaacgcata attctctccc atagtcgaac ctatgaggag gataagaaag cggaggaaaa
     3121 ggcggataaa aaatctaaaa cctcttccac acaaaaagct gttaaaaagt tgaatgctaa
     3181 tatagaaaaa actaccttag gggatatttc cgagctggca gctttaaaga ccgaacttga
     3241 ggaaaacagc aaaaaggaag caaaggccaa gaaggagacc aagcctaaaa aggagaccaa
     3301 gacagctaaa gcagctgcga gtgaggatga aaacgtaaaa gtggacgaac ccaaggccga
     3361 tgccgaagca aaggacgaac ccaaagttga tgagtagcgg caaagtataa ccatttaatc
     3421 ctgaaaacga tggaatttaa aattgagaaa tttgataagt ttacgctggt tgaggtgttg
     3481 gaggaaaaac ttgacaccaa catcgcccca gggttaaaat cagagttggt tttaatatcg
     3541 gggaatggtg agcgtaatct gatactcgac ctgagtacct gtcgttactg cgattcatca
     3601 ggattaagtg ccatacttgt tgctaacagg ctttgcaaga atgcgggtgg cacctttgtg
     3661 ctcaccggtt tgactgatgc ggttgaaagg ctaattacca tatcccaact cgatacggtg
     3721 ctcaacattg cctactccat ggaggaagcc gtgcagatta tagagaatgc agaaatggag
     3781 agcagcaaga agtgaccttt tctgtaacca tcttgggaag tagctcggct atccctacac
     3841 caacccgatt tccatccgct catgtgctca acgtgcatga gcggttgttt ttagttgatt
     3901 gcgccgaggg cacacagatg cagctccgcc gctttcatat ccggatgaat cgtattaacc
     3961 atattttcat cacccacctt catggcgatc acgttctggg ccttttcggc ctgatttcaa
     4021 cctttaacct attaggtcgg aagcagcccc tgcatctgtt tgcccctaag gagctggaaa
     4081 gcgtgctaaa cttcaacgtg ggtgttttta ccgataaact ctcgttccct gttattttcc
     4141 atccggttga ccccaacatg tcgcagctgg tttttgagga taggcatgtc agcgttacct
     4201 cgctacccct taggcataaa gttcctaccg tgggttatct ttttaaggaa accccctcgc
     4261 tacccaacat acgaaaggac tgcattcagc aatactcact gggcttggcc gatattgtaa
     4321 aaattaaaaa cggcgctgac tatatcgctc ctgatggcaa aatcattcca aatgcggagt
     4381 tgaccttcca tgcccggttg ccccgctcat ttgcatactg ttccgatacc ctctacagcg
     4441 agagactggt tgggctgatt agaggtgtgg atttacttta ccatgaggct acctttctgg
     4501 atgcagatgt agatctggcc cgtcaaacgg gacactccac tgccttgcaa gctgcaacag
     4561 ctgccgttgg ggcgcacgtt gggaagctgg tcattggaca tttttcatcc cgttacaaga
     4621 acatttcgca acacctgcag gaggcaagaa atattttccc tgaaacctat gctgccgaag
     4681 atggccaagt atatgccatt cccgaaaaat tcggctaacc ggatgcttgt tacaacagaa
     4741 ttttaacccc aactaccata ttcctagttc atttaaggtt tattttatac ctttgcaaat
     4801 tctgaaaata gtcatttttc aatgtacgaa aaaatcgtta tactcgattt cggctcacag
     4861 tacacgcagc taatcgcccg ccgaataaga gagttaaatg tttactgtga aatatacccc
     4921 tacaaccaaa tcccggtgtt ggatggcaca attaaaggag taatcctttc gggaagccca
     4981 ttttccgtga atgataacgc ctctcctaaa cccgatttga gcggaattag ggggaaattg
     5041 ccaattctcg gaatttgcta tggagcccag ctacttgccg ccagcagcgg gggccaggtg
     5101 atgccttcta aaagtaggga gtatggacgt gctacacttg aagtaatgga tgtttcatct
     5161 agcttattca aagatttccc catacagtcg caggtttgga tgtcgcatgg cgatactatc
     5221 accgcgctcc cccgtaactt tttgattact gccagcacca cggatattca ggttggcgca
     5281 tttcaggtaa caaacgagca aacctatggt attcagttcc atcctgaggt ttaccatact
     5341 acagagggaa ttaccctact ccgaaatttt gtggtggaca tttgcggttg cagccaaaca
     5401 tggacacctg catcgtttat tgaggaaact accagaaatc ttcgggaaac tctgggcagc
     5461 gataaggtag tattaggact atcgggtggg gttgactctt cagttgctgc cctgcttttg
     5521 cacagggcaa ttgggaaaaa tctaacctgc atttttgttg acaacgggct tcttcgtaaa
     5581 aatgagtacc agcaggtgct cgactctttt ggcagcatgg ggcttaacat agttggagtt
     5641 gatgccagcc agcagttcta caacgagcta aaaggtgtta ccgatcccga atccaaacgt
     5701 aagattattg gccgtaactt tattgaggtc tttgagcgtg aagctcaaaa aattcaaggg
     5761 gtcagctggc tcgctcaggg aactatctac cccgatgtaa tagagtcggt ctcggttaat
     5821 ggcccttcgg ccaaaataaa atcgcaccac aacgtgggcg ggttgccaaa ccagatgaac
     5881 cttaaggttg ttgagcccct tagacttctt ttcaaggacg aagttcgtag ggttggtaag
     5941 gagttggggc tttcggctgc aatacttaac aggcatccct tcccgggccc aggactgggc
     6001 attagggtga taggcgaggt taccccccaa agagttaagc tgcttcagga ggcggacaac
     6061 atatacatca aggccctgag ggagtcggaa ctatacgata agatatggca ggcaggtgcc
     6121 atactacttc cggtgcagtc ggttggagtt atgggcgacg aacgtaccta cgagaatgtg
     6181 gttgccctta gagctgttac ttcaaccgac ggaatgaccg ccgattggta cccatttccc
     6241 cataattttc tgtccagggt atctaacgaa atcattaaca aggtgagagg gataaacagg
     6301 gtggtttacg atatcagctc caagcctcct gccacaattg aatgggagta ggcttactct
     6361 atgaactttt accatttgtt tcgggtttta ctacatgtag aaattttcct ttaccatgac
     6421 taaaaaacta ctctatctat gtgcttttgc cctgataatg ccttttgggg cactatcgca
     6481 gaactcttca agctttggag cctacaagtt tgttcggttt attcagtacc tatcgggcga
     6541 ttacgttgat actatcaacg ttaacaagtt ggttgacaat gccattgttg aaatactctc
     6601 acagctcgat ccgcactcca tctatatttc aaaagaggat gttcaggcta tgaacgagcc
     6661 ccttgaggga aatttcgagg ggattggtgt ggagttcaac attctgaacg atacccttat
     6721 ggtggttaat cccatttcgg gcggaccatc tgagagggtt ggcatactag ccggcgacag
     6781 aataattgcc attgatggca agtctataac caatatcaac ctgaaaatca ccgatgtgca
     6841 taagttgctg cgaggcccaa agggaaccaa ggtaaccgtg ctcattcatc gtaagagcct
     6901 tagtacacct gtggagttca ccatcacccg cgaccgtatt ccaatctata gcatcgatgc
     6961 ctcctacatg gccgagcagg gtatcggcta catcaagatt aaccgatttg ccgctaccac
     7021 cgagcaggag tttgttgagg ctatgaacaa gttgaaccag caaaaaatga acgacctgat
     7081 catagacctg agaaacaacg gtggaggact gcttaactct gcttacgacc tggccaacta
     7141 ctttttcgac cccggcaagc tgattgttta caccgagggt cgtaatagcc caagaaccaa
     7201 ctatttttcc acttctaaaa ataaggtatt caggggaagg gtggtagtgc tcattgacga
     7261 gggatcggcc tcggcaagcg agattcttgc cggagcattt caggactggg ataggggaat
     7321 tattgttgga aggcgatcgt ttggtaaggg gttggtgcaa aatcagctgc ccttgcccga
     7381 tggttccatg attcgactca ccgttgcacg ttacttcacc ccaaccggta gagccattca
     7441 acgccagtat aaatttggtg atctgaagga ctactattcc gatatgactc agcgttacga
     7501 caggggcgaa ctcttcaaca aggatagcat tcattttgcc gattcgctta cctataaaac
     7561 cctcatcagg cataaaacgg tttatggtgg cggaggcatc atgcccgacg tgtttattcc
     7621 gctcgacacc aactactatt ccgactacta cggtaagctg gttcgttcgg gagttatcaa
     7681 ccaattttcc ttaacctggg tagataccca caggaatgag cttttaaaag aataccccaa
     7741 gttcgacaag tttaaaaaga attttcaggt aactgaaaag atgatctccg agctggtggc
     7801 tttagccgag agtagcggaa ttcccagcga tgagaaaggt ttagcccagt cggccaatga
     7861 acttaagata cagctcaagg ggctggttgc ccagtcaatt ttctctacca gccactactt
     7921 tgaggtaacc aatgaaacca atgaaactta tcaaaaagca ttggaaatat ttcatagctg
     7981 gaacgactac aagcatttag tacaggaata acataaattt aattaatttt actaataatt
     8041 taactggact ctatgaatga gtccaactta tcattacatt aatggaagca cttgatgaat
     8101 ttaaagagct cgggttatcc gaaaaggcat tggcagcaat aaaagctaag ggtttcgagc
     8161 agccttcgcc aattcaaaaa attaccatac cggtactttt aaaggagaca accgatgtta
     8221 tagcccaggc tcaaacagga acaggaaaaa cagcggcatt tggactaccc attatagaaa
     8281 tgttcgaggg ccagcctgca ggcataaaag cccttatttt ggtaccaacc cgggagttag
     8341 cccttcaggt ttgcgacgaa ataatttcgc tggctgccaa acgtatagag gtggttgcag
     8401 tttacggcgg ccaatcaatc tctgagcagc agcgcaggat tagaagaggt gttgatgtgg
     8461 ttgtgggaac cccgggtaga attcttgatt taattgcccg caaggacctt aactttcaga
     8521 acctgaaaat tttcgttctc gacgaggccg acgaaatgct caacatgggt tttatagaag
     8581 atattgaagc catcatggag catacgccaa aggaaaaacg caccctgctt ttctcggcca
     8641 ccatgcccga tagaattgtt aagctggcca agaagtacat gaagtcggct gttaagctcg
     8701 aggtggacaa ggcacagcca actactggcc ttaccgatca aatttacttc gaggttagag
     8761 attccgataa gtttgatgcg ctaacccgca tcatagacat cgaacctgaa ttctacggcc
     8821 tgatattctg ccgtacccgt aacgatgttg actcgctcac ctccaagctt attgagcgtg
     8881 gctatgccgc cgaggggttg catggcgaga tcacccaaag ccagcgtgaa aaaacccttt
     8941 taaaattcag gagacgtttg ctgaacatcc tggtggcaac cgatgtggct gccagaggaa
     9001 ttgacattat cgaccttacc cacgttatca actacaacat gcctcaggat gccgactcct
     9061 atattcacag gattggacga accggaagag ctggcaagca gggcacggcc attaccttta
     9121 tttctcacga tgagtaccgc aagttgaact atgttcaaaa ggccaccaaa tcgaatatcc
     9181 gtaaggaaat tctacctgct gccgaggata tcgttgccgt taaaaaggaa aaaattaagg
     9241 acgaactcca cgaaattatc aagcagggct catataacga ctaccttgga atggccgaag
     9301 acatccttga aactaatagc cccgaggtgg cgttggctgc cctgttgcgc ctagcctaca
     9361 aatcgcaact cgatgtctcc aactactccg aggctcgtac ttttagggtg gatactaaag
     9421 gcacaacccg cttgtttatt ggacttggtc gtaatgatgg catgacccca aaaaagattt
     9481 cttccttcat caggcaggag gttcaaatcc ccgataataa gattgacgac attgttgttc
     9541 gcgatgattt ttcctttatt accgtgccct ttgtggatgc cgaaaaaatt ctgaaggcct
     9601 ttgccaatca ggaaacagat ggtaaacccc ttataacaaa agccaaacca aaaaaataag
     9661 cgagtaggcc tttatggtgg aaaagtttga aatgattgcc aaaaccctcc aggggctgga
     9721 gggtgttctt gccgatgaac tcaccgaact tggtgctgag gatgtgaaga tcgaaaatag
     9781 ggccgtgagc ttccggggaa caacggaaac cctttacaag gctaacttct ggttacgaac
     9841 agccattcgg gtgcttaagc ctgtttactc atttagcgcc cgaaccattg aggagttcta
     9901 cggaaatgcc cgtaagttcg actggacttc actgatggac ttatcgcaaa aatttgcagt
     9961 tgacagcgtg gtcaactctg aggttttccc acattcacgt tacattgccc ttaaatttaa
    10021 agatgctgtg gttgatcagt tccgcgatcg ctacggaaaa cgtccgtttg ttgataccaa
    10081 aaatccccaa atacagttcc acgtccacat ttcgggcgat agctgcacca tcctgctcga
    10141 ttcctcaggg gaatcgttgc accgtcgcgg ctaccgaact gctcaggata ttgcccctat
    10201 caacgaggtg ctggcagcag ggctaatcaa actttcgggt tggaatatgg attcaacctt
    10261 tattgaccca atgtgcggct ctggaactat tgttattgag gcagccctta tggcgtatgg
    10321 aattgcaccc ggtgtttttc gccggcagtt tggttttgaa aactggctcg attttgatga
    10381 gaaacttctc cagtcaatct ataacgacga tagcatggag cgggagttta cacatccaat
    10441 tattggacgt gatatctcgc gccagaccgt tgaatcggcg ttgctcaacg ttaagcatgc
    10501 aggtttgcag aaaaaagttg acattgaagt aggctccatc ttcgactttc agcctcctgc
    10561 cgattctcag ggcattgttg ttaccaatcc accttacggc gaaaggcttc gacgtgagca
    10621 aatcgaaact ttctacaagc agctgggcga ctgctttaaa cagaaatatg aggggtacaa
    10681 cgtttggatg atcagctcga acatggatgc cctgaaaaat tttggtttgc gaccatcaaa
    10741 acgctacacc ctctataacg gcgctcttga atgtaagttc caacgctact ccatgtttgc
    10801 cggtagtgta aaggcgaagt accagggtaa aggggaaagg ataaagagta aggatagaga
    10861 ataagataaa cttgtccgcc taccggcgga acttgtccgg ctcccgacgg attcaccctt
    10921 cgacaggtgt tacagtgagt ttgtcgaact gctcagggtt cagacttgtc cacctactgg
    10981 cggatttcgg aact
//
LOCUS       DAOPHX010000088        10967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_7279_length_10967_cov_5.215543, whole genome shotgun sequence.
ACCESSION   DAOPHX010000088 DAOPHX010000000
VERSION     DAOPHX010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10967
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7279_length_10967_cov_5.215543"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2361
                     /locus_tag="PK502_07460"
     CDS             <1..2361
                     /locus_tag="PK502_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPH00691.1"
                     /translation="PEVFYTSTIHGDEVTGFVLMLRLADYLLSQYGSNSRITNLLNGT
                     AIYINPNANPDGTYYSGDSTVYGARRDNQNNVDLNRNFPDPRAGDHPNGNSWQPETQA
                     MMNFANEHHFILSANFHGGAEVANYPWDTWTSAENTHADNSWYYTISRAYADSVHAHG
                     PAGYFTDENNGVTNGGDWYVITGGRQDYMNYWHHCREITLEISSTKLLSSDELPAYWG
                     YNRESLIGYLEHALTGFYGTVTNPDGQPLDATITIPGHDRDSSHVVTNPEHGSYYRMI
                     APGSYEVLYQSDGYISQIHTVSVPSYSSRIMKDVVLEPAQQVNLSGLATDSLTGNPIE
                     GAKVELVGSSYTPVYTNAQGIYVFSAIYEGYYQVRASKAGYIPSTQELNLTVSSNTLN
                     FILAPSEAESFELQLPSTLTFTGGSWSRTNQTAYTGSYSLRSASIGHSSSTAMTATQN
                     FNGPGEVAFARKVSSEQGYDFLRFYIDGTKKGEWSGEQSWGEESFSITSGTHTLKWEY
                     KKDEMVIGGSDCAWVDDIILSPTLQNATFTVLVNGNEYEGLTVNLDGMTANTGSDGKV
                     NFVNVPTGLAKPVSVQYMGNTLADSLVNVVWTGQNSYDMDISAYFEVTFHVTSEGNPV
                     EEATVTLNDLQLQTNSQGIATFTGIPFAPNIHVDISRSGYNTYEGDISIASDSTYTIS
                     LNPNASEPIIGNTQLLRIFPNPFTDRVNIEIETSEYSQVQLEVYSILGCRMDNVFNGM
                     LTPGIHNFTWKYDNMPKTQSSGNIFMVWVRVNDTIIVRKIVQIQQK"
     gene            2435..4090
                     /locus_tag="PK502_07465"
     CDS             2435..4090
                     /locus_tag="PK502_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPH00692.1"
                     /translation="MHLKRLSLLILFTAGLRLSLWAQPNRQIPSEKPRLIVEIVASQM
                     RFDYLTRYWDKFSDGGFKMLVNDGASCKNARYSYLLTQAYPGIATISTGANPSIHGII
                     SDSWFSRVNGEVVDAVADEKTTTTGGSFASGRASAKNLVTSTLGDEICLNNPQSKVVS
                     IALDAGSAILMAGHSASGIYWLDTEKGGWVSSSYFSDSLPAWVDTLNAKSLAKLYAEK
                     EWKAINPIQAYEETDTLTARTPEGKKRVSDKIKLLIDGIIGKSEPATSYKKLLETPFG
                     NSLTKDMAIASILGESLGSDENTDLLCITFSANRYITRKFGPHSIEMEDTYLRLDREL
                     AHFLQFLATQVGKENVLVVLTSDQGVSSTPEFLEKSKIPGGYFDPKKAMVLLGSYLNA
                     LYGQGSWITGYHEKQIYLNRRLIEDSNINLAEMQQKVADFMLEFSGVANSATAHTLQS
                     ANFANGIMVKFQNSYNQRRSGDVIINLEPGWVERNGSITSGNSAYDYDTHVPLIWYGW
                     KVKRRVITSPVNIYDIAPTLSSLLGITWPNGSMGSPVRELVEP"
     gene            complement(4114..4827)
                     /locus_tag="PK502_07470"
     CDS             complement(4114..4827)
                     /locus_tag="PK502_07470"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HPH00693.1"
                     /translation="MTNIYSSLFSSGKSRQIALLLDPDKGNELSLSKTLELANQTRVS
                     MILVGGSLISGDINSFVLRVKEHTALPVILFPGSAFQFTSHADAILLLSLISGRNPEL
                     LIGSHVQVAMRIKQSGMEVIPTGYILVDGGAVTSVQYMSNTMPIPSDKPDIVVATALA
                     GQQLGLKLIYLEAGSGAKNSVPAELVRQVRSQISIPLIVGGGLRSARQVDAVFAAGAD
                     MVVVGNSVENNPAILEELG"
     gene            complement(4839..5450)
                     /locus_tag="PK502_07475"
     CDS             complement(4839..5450)
                     /locus_tag="PK502_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HPH00694.1"
                     /translation="MPIIKEILNEGNRIVVWQFHDGEFESPALQHESISDAVAHPKRI
                     LERQAVLAILKYMGFANGYSYTPESRPFIPGSNQHISISHSGSVAVVAVNKSTPVGVD
                     VEQLDRPFDRVAKKFLSKEEHLVVNVHDKSELALIWCVKEATYKLPWAKPCSLADDMV
                     VTLDRKQMESGWCRVKVRNCGSVAELKANFTFNGGYCLAWVNR"
     gene            complement(5466..6044)
                     /gene="gldD"
                     /locus_tag="PK502_07480"
     CDS             complement(5466..6044)
                     /gene="gldD"
                     /locus_tag="PK502_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HPH00695.1"
                     /translation="MSNRTPFSLLAILFLSVMLFGCADPPVPKPRGYFRIDFPAKSYR
                     LFDSIYPFRFMYPTYSVIIPKESADSGVWLNVDFTGYKATLHLSYKNVHGDISKMIED
                     ARSLAYKHTVKADAIDEQFFSNQQEKVYGVLYDIRGNSASSVQFFVTDSTQHYLRGAL
                     YFRCVPNKDSLAPAVDFFKKDVVHLLETLSWK"
     gene            complement(6022..7329)
                     /gene="gldE"
                     /locus_tag="PK502_07485"
     CDS             complement(6022..7329)
                     /gene="gldE"
                     /locus_tag="PK502_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HPH00696.1"
                     /translation="MDIAFHPITVGTVVGLLVLILLLIISGLMSGSESAYFSLSPADL
                     KKLESKSHQRAQSFVLINLNESERLLATILIVDNLVNVGIVILSTYLTNSLIDFSNAP
                     VAGFIFQVIVITFILLLFGEVMPKILATNRALKFALFMAYPLHLLVRLFRPLSSLLLS
                     STSRIGKRASTHNQISMDELSNALEITSDSIAEEKQILQGIVTFGSTIVSEIMKPRID
                     VVAVEYDTSFNHLKNVVVDSGYSRIPVYSETFDSIRGILYVKDLIPYIDREDDFHWQN
                     LIRTPYFVPESKRINDLLSEFQLKRIHMAIVVDEYGGTCGIITLEDILEEIVGEISDE
                     SDDETPLYTVVDSSTYIFEGKILLNDFCKVIGCSDDVFDDVRGEAETLAGLILEITGQ
                     FPSRNDVVTCKQFEFTVDMVDSRRIKRVKVKVHDREDEQSNSI"
     gene            complement(7493..7930)
                     /gene="ssb"
                     /locus_tag="PK502_07490"
     CDS             complement(7493..7930)
                     /gene="ssb"
                     /locus_tag="PK502_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPH00697.1"
                     /translation="MSVNKVILVGNVGKDPDVRHLEGGVTVARFPLATNESYTDKSGK
                     KVTQTEWHNIVVWRGLADVAEKYVRSGKLLYVEGRIRTSSYDDKEGNKRYSTEIVCDN
                     FRFLGPNVNQQEHSTPYPEQVSDAKSNLSDVENIPSPDDDLPF"
     gene            complement(7994..9067)
                     /gene="mutY"
                     /locus_tag="PK502_07495"
     CDS             complement(7994..9067)
                     /gene="mutY"
                     /locus_tag="PK502_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPH00698.1"
                     /translation="MDFSALLLEWFSVHHRDLPWRNTSDPYHIWVSEVILQQTRVNQG
                     TEYYQRFIHIFPDIPALAEAPLDAVMKAWQGLGYYTRARNLHAGAKQVMSEFGGKLPT
                     SYSELLRIAGLGPYSAAAVASFAFNEAVPAVDGNVYRILARVFGVFTPVNSSQARREF
                     FNLAGELIDRNRPAEFNQAIIDLGALVCTPRRPSCSACPFSDYCYAYLNNLTGLLPLK
                     NRKNPPRSRYFTYLMINFNGTTFIRKREGNDIWHSLYEFPLIETPEQLDTTQLVETEA
                     WKELMGDSSAEIIHVSDPIKHQLSHITLHARFIIVQMRNPTYALRTGYRNISIGNLQE
                     LSVPRLIHSYMASEPAERYFTGK"
     gene            9203..9490
                     /locus_tag="PK502_07500"
     CDS             9203..9490
                     /locus_tag="PK502_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPH00699.1"
                     /translation="MTKADIVNEISKTTGIEKVEAQKVVEAFMEIVKDSLAKEKNVYL
                     RGFGSFIVKKRAQKTARNISKNTTIIIPEHYIPSFKPAKTFVNKVKNHVKK"
     gene            9727..>10967
                     /locus_tag="PK502_07505"
     CDS             9727..>10967
                     /locus_tag="PK502_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPH00700.1"
                     /translation="MNTELVIDVQEAEIAIALLENKQLVELNKEKGNVQFSVGDIYLG
                     KVKKIMPGLNAAFVDVGYEKDAFLHYLDLGPQFKTLQQYVQAHYARRTKTIPVPKFKL
                     EPDIDKNGKITDVLSSGQAVLVQIAKEPISTKGPRLTSEISIAGRNLVLIPFSDKVSI
                     SQKISSNEERKRLKRLMESIIPKNYGVIVRTAAEGKKVAVLDAELRSLIKKWETATEK
                     LKTVSFPGQLASELKRTSAILRDMLNGTFNSIHVNDEIVFNDIHEYIETIAPEKEKIV
                     KLYTGNVPIFDQFGIEKQIKSLFGKTVSIKSGAYLIIEHTEALHVIDVNSGNRSKSGN
                     DQETNALDVNLAAADEIVRQLRLRDMGGIIVVDFIDMHQNENRNMLFERMKELMSKDR
                     AKHNILPLSKFGLMQITRQRVR"
BASE COUNT         3181 a         2632 c         2525 g         2629 t
ORIGIN      
        1 cccgaagtgt tctacaccag caccatacac ggcgatgagg taacgggatt tgtgctgatg
       61 ctcaggctgg ccgactacct gctcagccag tacgggagca actcccggat aaccaacctg
      121 ttgaatggca cggccatata catcaatcct aacgccaacc ccgatggaac ctactacagt
      181 ggagatagca ccgtttacgg tgcacgacgc gacaaccaaa acaacgttga cctgaaccgt
      241 aactttcccg acccccgcgc aggcgaccac cccaatggca actcatggca gcccgaaacg
      301 caggctatga tgaactttgc caatgagcac catttcatcc tatcggcaaa cttccacggc
      361 ggagccgagg tggccaacta cccgtgggac acatggacat cggccgagaa tactcatgcc
      421 gacaacagct ggtactacac catcagccgg gcctacgccg attcagtcca tgctcacggc
      481 cctgcaggct attttacgga tgaaaacaac ggggttacca acgggggtga ctggtacgtg
      541 ataaccggcg gacggcagga ctacatgaac tactggcacc actgccggga gattaccctg
      601 gagatttcca gcaccaagct gctcagctcc gatgagctgc cggcctactg gggctacaat
      661 agggaatccc ttatcggcta ccttgagcat gcccttaccg gtttctacgg cacggtaacc
      721 aatccggatg gacaacccct cgatgccacc atcacaattc ccggccacga cagggatagc
      781 tcgcatgtgg ttacaaaccc tgagcatgga agctactacc ggatgattgc cccaggaagc
      841 tacgaggttc tttaccaatc ggatggctat attagccaaa tccacactgt aagcgttccc
      901 agctattcaa gcagaattat gaaggatgtt gtgcttgagc cagctcagca ggttaacctt
      961 tcaggattag ctaccgacag cttaaccgga aaccccatcg aaggggccaa ggttgagctg
     1021 gtgggttcct cctacacacc ggtttacaca aatgctcagg gcatttatgt cttctctgcc
     1081 atatacgaag gctactacca ggtgagggca tccaaagctg gctacatacc ttcaactcaa
     1141 gaattgaacc tgaccgtctc cagtaacacc ctgaatttca ttctagcccc ctctgaggca
     1201 gaaagctttg agctgcagct accctccacg ctaaccttta ccggcggcag ctggagccgt
     1261 accaaccaaa cagcctacac gggcagctat agcctacgct cggccagcat cggccacagt
     1321 agcagtacag ccatgacagc tacccagaac ttcaacgggc ctggggaggt agcctttgcc
     1381 cggaaggtct cctccgaaca gggatacgat tttttgagat tttacattga tggtaccaaa
     1441 aaaggagaat ggagcggtga acagagctgg ggtgaggagt ccttcagcat cacctccgga
     1501 acccacaccc tcaaatggga gtacaaaaag gatgaaatgg ttataggtgg aagcgattgc
     1561 gcatgggttg acgacatcat cctctcccct accctgcaaa atgccacctt taccgtgctg
     1621 gtcaatggaa atgaatacga gggtttaacc gtcaaccttg atggtatgac ggcaaacacc
     1681 ggaagcgatg gcaaggttaa cttcgtcaac gtgcccacag gtttggcaaa accggtgagt
     1741 gtacaatata tgggaaacac cctggccgac agcctggtga atgtggtttg gacaggccaa
     1801 aatagctacg acatggacat ttcggcatac tttgaggtaa catttcatgt aacctcggag
     1861 ggaaaccctg tggaagaggc aaccgttacc ctgaacgacc tgcaactaca aaccaacagc
     1921 cagggaatag caacctttac cggcattcct tttgcaccaa acatccatgt tgacatatct
     1981 cgaagcgggt acaacaccta tgaaggtgat ataagcatag caagcgactc tacctacacc
     2041 atctctctta acccaaatgc ttcagaacct attataggta atacccaatt gttaagaata
     2101 tttcctaacc cctttaccga tagggtaaac atagaaattg aaacttctga atactcccag
     2161 gttcagctgg aagtgtatag tatccttggc tgcagaatgg acaatgtatt caacggtatg
     2221 ctaacgccag gcattcacaa cttcacgtgg aaatatgaca atatgcctaa aacacagtca
     2281 agtggaaata tctttatggt ttgggtaaga gtaaatgaca caataattgt tagaaaaata
     2341 gttcagattc aacaaaaata agttgctttg taccggcatt cccaagcaac ttggggaaaa
     2401 aaccattacc aattttttag aagttccata aaccatgcat ttaaagagac ttagcttgtt
     2461 aatcctgttc actgcaggac ttaggttatc cttatgggca cagcccaacc gtcaaattcc
     2521 atcagaaaaa ccacgtttga ttgtcgaaat tgtagctagc cagatgcggt tcgactacct
     2581 gacaaggtac tgggataagt tttccgatgg tggatttaaa atgctagtta acgatggtgc
     2641 cagctgcaaa aatgcaaggt acagctacct tttaacccaa gcctacccag gaattgcaac
     2701 aatctccact ggcgccaacc catcaattca tggcataata tccgacagct ggttctcgag
     2761 agttaacggc gaagtggttg atgctgttgc cgacgaaaaa acaaccacta cgggtggcag
     2821 cttcgccagc ggtagggcct cggcaaaaaa tcttgttacc agcactctgg gtgatgaaat
     2881 ttgcctaaac aacccccaat cgaaggtggt gagcattgct cttgatgccg gttcggccat
     2941 actcatggcc ggacatagcg cgagtgggat ttactggctc gacaccgaaa agggtggatg
     3001 ggtttccagc agctacttta gcgacagcct acccgcatgg gtggataccc ttaacgctaa
     3061 atcgcttgca aagctttatg ccgaaaagga gtggaaagcg ataaacccca tacaggctta
     3121 tgaagaaacc gatacactca ccgccaggac tccagaggga aagaaaaggg tatcggataa
     3181 aattaagcta ctgattgacg gcataatagg aaaatcggaa cctgctacca gctacaaaaa
     3241 gctgcttgaa accccatttg gtaactcgct taccaaggat atggctatag cctccatctt
     3301 aggtgaaagc cttggtagcg atgaaaacac cgacttactc tgcatcacct ttagcgccaa
     3361 ccggtacata acccgaaagt ttggcccaca ttccattgaa atggaggata cctacctgag
     3421 gcttgaccga gaactggcac acttcctcca attcctggca acccaggtcg gaaaggagaa
     3481 tgtgctggtg gtgctcacct ccgaccaagg ggtttcgtca actccggaat ttctggaaaa
     3541 gtcaaagatt ccaggtggat acttcgatcc taaaaaagcc atggtgctgc tgggtagcta
     3601 cctgaatgcc ctatacggac agggcagctg gataacaggc taccacgaaa aacagattta
     3661 cctcaaccga aggttgatag aggattccaa cataaatctt gccgaaatgc agcaaaaggt
     3721 tgccgatttt atgcttgagt tctccggtgt ggccaacagt gcaaccgctc ataccctcca
     3781 atcagcaaat tttgcaaacg ggataatggt gaaatttcag aatagctaca accaacgccg
     3841 ttcgggtgat gttattatta acctggagcc gggatgggtt gaacggaatg ggtccattac
     3901 ctccggcaac tcggcctacg actacgatac ccatgtaccc ctaatctggt atggctggaa
     3961 ggttaaacgt cgcgttatta cgtcaccggt aaacatttat gacatagctc ctaccctttc
     4021 gagcctgctg gggataacct ggcctaacgg ttcgatgggt tcccctgtaa gagagctggt
     4081 tgaaccctaa gttgtgccct tcagtaagca ttgctagccc aactcttcga gtatggcagg
     4141 gttgttttca acgctgttgc caactactac catgtcggca ccggctgcaa aaaccgcgtc
     4201 aacctgcctg gcgctgcgaa ggccccctcc aacgattaga ggaatggaaa tttggcttcg
     4261 cacctgcctt acaagctcag ccggaacgga gtttttagcc ccactacccg cctcaaggta
     4321 aattagcttt agtccaagtt gctgtccggc taaagccgtt gctacaacaa tatcgggctt
     4381 atcggatgga ataggcatag tgttgctcat gtactgcacc gaggtaacag cgccaccatc
     4441 aaccagtatg tacccggtgg gtataacctc cattccgctc tgcttaatcc tcattgccac
     4501 ctgcacgtgg ctacctataa gcagctcggg gttacgtccc gaaataaggg aaagtaaaag
     4561 aatggcatca gcatgggagg taaactgaaa agcactaccc gggaataaaa ttacaggaag
     4621 ggctgtatgc tctttcaccc gtagcacaaa actgttaata tcgccactta tcaggctacc
     4681 tccaaccaaa atcatggaaa ctcgggtttg gttggccaac tccagagtct tgctgaggct
     4741 taattcgttt cccttgtcgg gatcgagcag cagggctatc tgccttgatt ttccgctact
     4801 gaataatgag ctgtaaatgt ttgtcataca ccaaaatgct accggttaac ccatgccagg
     4861 caatagccac cattaaaggt aaaattagct tttaactcgg ctacgctacc gcaattcctc
     4921 actttgaccc tgcaccagcc actttccatc tgtttcctat cgagcgtaac caccatgtca
     4981 tcagccaggc tgcaaggttt agcccatgga agcttgtagg tagcctcctt tacgcaccaa
     5041 atcagcgcca actcactttt atcgtgaacg ttaactacaa gatgctcctc tttggagagg
     5101 aatttttttg caaccctatc gaatggtctg tccagttgct ccacgtcaac cccaaccggc
     5161 gttgatttat ttaccgccac caccgccaca ctgccggaat ggcttatgga gatatgctgg
     5221 ttggaaccgg gaataaaagg acgcgattcg ggggtgtagg agtagccatt ggcaaatccc
     5281 atgtatttta gtatagccag aacagcttgt cgctcaagaa tacgttttgg gtgagcaacg
     5341 gcatcgctta tgctttcatg ctgtagcgca ggagactcga actctccgtc gtggaactgc
     5401 caaaccacaa ttcggttacc ctcgtttaga atctccttaa taattggcat atacttaaat
     5461 ttgagctact tccatgaaag ggtttcaagc aggtgaacta catccttctt aaagaaatca
     5521 acagctggag caagagaatc cttgtttgga acgcacctaa agtaaagggc accacgcagg
     5581 taatgctggg tggagtcggt tacaaagaat tgaacggagc tggcagagtt gccgcgtata
     5641 tcgtatagaa ctccgtaaac cttttcctgc tgatttgaaa aaaactgctc atcaatagca
     5701 tcagccttta cggtgtgctt gtaggccagg gagcgggcat cctcaatcat tttagaaata
     5761 tcgccatgca cgtttttgta gctcaggtgg agtgtagcct tataccctgt aaagtcaaca
     5821 ttaagccata caccagaatc agcgctttcc ttaggaataa tcacagaata ggtagggtac
     5881 atgaagcgaa aagggtaaat agaatcgaag agccggtagc tttttgccgg gaaatcaatc
     5941 cgaaaatagc cccttggttt cggaaccgga ggatcggcac aaccgaatag cataacagaa
     6001 agaaacagga ttgccagcag gctaaatgga gttcgattgc tcatcttctc tatcatgcac
     6061 tttaacctta acacgtttta ttctccggct atcaaccata tccacggtaa actcaaactg
     6121 tttgcaggtt accacatcgt ttcgggaagg aaattgtccg gtaatttcaa gaatgagccc
     6181 tgcaagagtc tcggcctcgc cccttacgtc atcaaacaca tcgtcgctgc acccaataac
     6241 cttacagaag tcgttgagca gaattttgcc ctcaaaaata tatgttgagc tgtcaactac
     6301 cgtgtaaagc ggagtttcgt cgtcggattc atcagaaatt tcgcctacaa tctcctcaag
     6361 aatatcctca agggtgatta ttccgcatgt tccaccatac tcgtcaacca caatggccat
     6421 gtggatacgt ttcagctgaa actccgatag caagtcgttg attcttttac tctcggggac
     6481 gaagtaggga gttctaatca ggttttgcca gtgaaagtcg tcctcacggt caatgtaggg
     6541 gatcaggtct ttaacataga gtatgccacg aatgctatcg aaggtttcgg agtaaacagg
     6601 gatgcggctg tagcccgaat ccaccactac gtttttaagg tggttgaacg aagtgtcgta
     6661 ttccaccgcc actacgtcaa ttcggggctt cataatctcg ctcacaatgg tgcttccaaa
     6721 cgttacgata ccctgaagga tttgtttctc ctcggcaatg ctatcggatg ttatctccag
     6781 ggcgttgctt agctcatcca tggaaatctg gttatgggtg ctcgcacgtt tcccaattct
     6841 agaggttgaa gatagcagta gggagctaag gggcctaaac agcctaacaa gcagatgaag
     6901 gggataggcc ataaacaggg caaacttaag cgcacggttt gtagcaagta ttttaggcat
     6961 aacctcgccg aaaagcagca gaataaaggt gatgacaata acctgaaaaa tgaaaccggc
     7021 aacgggagca ttcgaaaaat caattaaaga attagtgagg taggtggaaa ggatgacaat
     7081 tccaacattc actaggttat caacaatcag aattgtagct agcaacctct cggattcgtt
     7141 aaggttgatc agcacgaaac tctgagccct ttggtgcgat ttgctttcca actttttcaa
     7201 atcggccggg gagagtgaaa agtaggccga ctcgcttccg ctcatcaggc cggaaatgat
     7261 aagcaaaaga atcaacacaa gcaggccgac taccgttcca acagtaatag gatgaaaggc
     7321 aatatccatt aatccaactc cttttaatat tcgccattct gcgatggctt cgacttgtaa
     7381 aattactaaa atattcagag attacgttaa tttagcatac agcagccatt taccaacaaa
     7441 aaaccgatag tttggttagg ctatcggttt ttggggcagg gataataagc agctagaatg
     7501 gcaggtcgtc atcgggcgaa ggaatgttct ccacgtcgga gaggttggat ttggcatcag
     7561 acacttgctc agggtaaggg gtggaatgtt cctgctggtt cacattgggg ccaaggaatc
     7621 gaaagttgtc gcaaacaatc tctgtgctgt atcgcttatt tccctccttg tcgtcgtagg
     7681 atgaggttct aatgcgacct tcaacataca acagctttcc agacctgaca tatttttcag
     7741 caacatcggc caaacctctc cataccacaa tgttatgcca ttcggtttgg gtaacttttt
     7801 tcccggactt gtcggtatag ctttcgttgg tagccagtgg gaaacgggct acagttactc
     7861 ctccttcgag gtggcgtaca tcggggtctt tgcccacgtt gccaactaga attaccttgt
     7921 taactgacat tgttttagat ttttggttat ttagaccaag gtagggtaaa aaaatataca
     7981 aagcaaataa atttcacttt cccgtaaagt agcgttcagc aggttcagaa gccatgtatg
     8041 aatgaattaa acgcggtacc gacaactctt gtaggtttcc aatgctaatg tttcgatatc
     8101 cggttcgtaa ggcataggtg ggatttctca tttgcacgat aataaaacgg gcatgcaggg
     8161 ttatatggct aagctgatgc ttgattgggt cggacacatg aataatctcg gctgatgaat
     8221 cgcccattag ctctttccac gcttcggttt caaccagctg agtggtatca agttgttctg
     8281 gagtttcaat gagcggaaat tcatacaggg agtgccatat atcgttcccc tcccgcttgc
     8341 ggataaaggt tgttccgtta aagttaatca taaggtaggt gaagtatcgt gatcggggtg
     8401 gattcttcct gtttttaagg gggagcaacc cagtcaggtt gtttaaatag gcatagcagt
     8461 agtcggaaaa agggcaggct gaacacgaag gcctgcgcgg tgtgcaaacc aatgcaccca
     8521 ggtcaataat agcctggtta aattcagcag ggcgattcct gtcaataagc tcaccagcaa
     8581 ggttaaaaaa ctcccttcgg gcctgtgagg agttaaccgg agtaaacaca ccaaacacac
     8641 gagccagaat tctgtaaaca tttccatcca cagcaggcac agcctcgttg aaggcaaacg
     8701 aagccactgc agcggctgag tatggtccga gtccggcaat acgcagaagt tcggagtagc
     8761 tggtgggcag cttcccacca aattcggaca ttacctgttt ggcacctgca tgcaggttgc
     8821 gggcacgggt gtagtatccc agcccttgcc atgccttcat aacagcatca aggggcgctt
     8881 cggccaatgc tggtatatcg gggaaaatat gaataaacct ttgatagtat tctgttccct
     8941 gatttaccct tgtttgctga agtataacct ccgaaaccca aatatggtaa ggatcggacg
     9001 tattcctcca gggtaaatca cgatggtgta cactaaacca ttccaggagc aatgcactaa
     9061 aatccataca atcagcactt agcaaaacaa attcttgtca aaattaatac ttgccgtttc
     9121 aattatcgag ataaaaggtt tatatttgca gttcgaaatc atccactatg tgtaacatat
     9181 tgataattaa atacttttag aaatgacaaa ggcagatatt gtaaatgaaa tttccaagac
     9241 taccgggata gaaaaggtag aagcacagaa ggttgtagaa gccttcatgg aaatagtgaa
     9301 ggactccctg gcaaaggaaa aaaatgttta cctcagaggg ttcggcagct ttattgttaa
     9361 aaagcgcgca caaaaaaccg cacgtaacat ctcaaaaaac accaccataa ttattcccga
     9421 acactacatt ccatcgttta aacccgccaa gacctttgta aataaggtga aaaatcacgt
     9481 taagaaatag gcaaccataa atactaacca aaaaaatttc acgtcatgcc aagcggaaaa
     9541 aaaagaaaaa ggcataaaat ggctacgcat aaacgcaaaa aacgtttacg caagaatcgt
     9601 cataagaaaa agaagtagca accctggttt agtaggaatc aggaaattat gcaaacaacc
     9661 taaagttcct gcatggcgct ttaggttgct ctatttgttg tatatacctt acctaaatta
     9721 tttaaagtga acaccgaact agtaattgat gtacaggaag ctgaaattgc cattgccctg
     9781 ctagaaaaca agcagctggt agagctcaac aaggagaaag gcaacgttca gttttcggtt
     9841 ggcgacatct atctcggtaa ggtaaaaaaa atcatgcccg gattgaatgc cgcattcgta
     9901 gatgtaggct acgaaaagga tgcattcctg cactacctcg acctgggtcc gcagtttaaa
     9961 acccttcagc agtatgttca agcccactac gcccgaagga ctaaaacgat acccgtaccc
    10021 aaatttaagc ttgaaccgga tatagataag aacgggaaaa ttaccgatgt actttcatcg
    10081 ggtcaggctg tgcttgtaca gatagccaag gaacccatct ccaccaaggg cccccggttg
    10141 acctccgaaa tatccatcgc cggtcggaac ctcgtgctga ttccattttc ggataaagta
    10201 tccatttctc agaagatttc ctcaaacgag gagcgcaaaa ggctaaagcg gctgatggaa
    10261 agcattatcc ccaaaaacta cggggtgatt gtgcgcactg ctgccgaggg caagaaggta
    10321 gcagttctcg atgccgagct tcgcagcctt atcaagaagt gggaaaccgc aaccgaaaag
    10381 ttaaaaacag tatccttccc cggccagctg gccagcgagc taaagcgaac ctcggccatc
    10441 cttcgggata tgctgaacgg aacgttcaac agcattcatg tgaacgacga gattgtgttc
    10501 aacgatatac atgagtatat tgaaaccatt gcccctgaaa aggagaaaat tgtcaaactt
    10561 tacacgggaa atgttcccat atttgaccag tttgggattg aaaagcagat aaaatcgcta
    10621 ttcggcaaaa ctgtttcaat aaaaagcggg gcatacctca tcatagagca caccgaggct
    10681 ttacatgtaa ttgatgttaa cagcggaaat cgttcaaaat ctggtaacga tcaggaaacc
    10741 aacgcactgg atgtaaacct ggcagcggcc gatgagattg tccgtcagct caggcttcgc
    10801 gatatgggcg gcatcattgt ggttgatttt atagatatgc atcagaacga gaaccgcaac
    10861 atgctgttcg aacggatgaa ggagctgatg tcaaaagaca gggctaagca taacatatta
    10921 ccgttgagca agtttgggct aatgcagata acccgccagc gagttcg
//
LOCUS       DAOPHX010000089        10915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_7327_length_10915_cov_5.867403, whole genome shotgun sequence.
ACCESSION   DAOPHX010000089 DAOPHX010000000
VERSION     DAOPHX010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10915
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7327_length_10915_cov_5.867403"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PK502_07510"
     CDS             <1..938
                     /locus_tag="PK502_07510"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPH00701.1"
                     /translation="LNLRTISGNMRLCKDEILAFKPDVLILVDYPGFNLRIARFAKKN
                     EIRVFYYIAPKVWAWNESRVKVLKKYVDTLFIIFPFEQEYFARHGINAVYLGNPTPDA
                     IEQKIGGKVVTFNQFVEDNKLSQKPIIALLAGSRKQEIRYNLPVMLRMVEKFPDYQLV
                     IAGAPSLSEDVYRPYVAGKNVAIVFDQTYQLLSCSTLALVTSGTATLETALLNVPQVV
                     CYRGNILSMLVAWVVIRVKYISLVNLNMQAEVVKELKQFDLTPENLYAEASKLLPGSP
                     EREKMLTSYAQLQKQMGGSGASMRVGDKIVELISR"
     gene            1161..2405
                     /locus_tag="PK502_07515"
     CDS             1161..2405
                     /locus_tag="PK502_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPH00702.1"
                     /translation="MQKLTFTFIVSLILTLGAAGQKISISLFNNLKLSTILITPTSGS
                     YRLILGDNEVELKVNQILYISRSGDSLMVRDANQNMGTWKRVTVVGQTGEDIIRVKPI
                     VPASPARLYDDNLSFYVDLDRIFAINLIDQEKYVAAVVEAEGGLNREPEFYKVQALIV
                     RTYTFAHLNKHLAEGFNLCDEVHCQSYLGRSEKNSDILDAVLDTKDMVIVDAERNPIT
                     AAFHANCGGQTANSEDVWVKPLPYLVSVSDRYCLGSNSSTWERSIPIGEWKKFLSQNG
                     VDTLKIKKNSDLDFQAKTRPKYYAVMGVEIPTTDIRKHFRLRSAWFSVSAGKSEIRLS
                     GRGYGHGVGLCQDGAINMAQKGWTYDQIVQYYYKGVSIVKLTELDMDRIRVDTTLNNM
                     VEPNLPPPGMEFPQRPPDRGTR"
     gene            2441..3172
                     /gene="gldF"
                     /locus_tag="PK502_07520"
     CDS             2441..3172
                     /gene="gldF"
                     /locus_tag="PK502_07520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HPH00703.1"
                     /translation="MFAIFTRELQNFFSSLSGYIAAMVFLVVTGLILWVFPGNLNILD
                     GGYASLNGYFSIAPWVLLLLVPAATMRTFSDEKRFGTLELILTRPISDGRIVLGKFFA
                     TLVLVLIVLVPSLVYYLSVYLLSSPIGNIDAGGTWGSFIGLFLLASTYASVGIFTSSL
                     TDNAIVSFLLSVLFCLFLFSGLSALASLPLFSGVKPLLMFLSISEHYASISRGVLDSR
                     DLVYFISMIFLFIIFTRTVLESRKW"
     gene            3197..4909
                     /gene="gldG"
                     /locus_tag="PK502_07525"
     CDS             3197..4909
                     /gene="gldG"
                     /locus_tag="PK502_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HPH00704.1"
                     /translation="MELKKSYKVKSLLRLLVSVVAVAALYALSSVLHFRIDLTSDKLY
                     TLQPITREILKNLNGEVSMKIYLDGDLPTGFIRMKRMLTETLDEFNVASRGKVRYELV
                     NPYSAKNSREQDRIFAGLYDKGLAPTSVQQRNRQGGSSQTIVFPGLLMSYRGKEMAVN
                     LLKNNPMLSAEENLNLSIQNFEFALVDALVKLSTDSLPRIAFIQGHGELDEFETGDIE
                     KSLSEYFRVDRVILNGDMEALKPYSVAIVAGPTKPFPEEDKLVVDQFIMRGGKVLWFV
                     DPVAVSLDSLSTGASTLAFVAQTNLDDMLFRYGVRLNPFIVQDLQCAVIPVNIAPSGQ
                     ASKFAPAPWPYFPLLIPPSNHPVTRNLNPILTRFVSPVDTVGENPLVKKHYLLYTSAT
                     SRILQVPLLINLSEIEQTPNEQHFINPNVPVAAELEGYFTSVFTNRPLKNYNHGEPFE
                     FRAKSSFTRMVVVADADMVRNDVQHRPNGAYVLPLGYDRYTQQTYGNKELVMNMVKYL
                     NDDNGIVNLRSRDFKLRLLDRSKASQSRLAWQVVNTVLPSLVLVLGGLIYFFIRRNHY
                     STPK"
     gene            5200..6330
                     /gene="dnaN"
                     /locus_tag="PK502_07530"
     CDS             5200..6330
                     /gene="dnaN"
                     /locus_tag="PK502_07530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPH00705.1"
                     /translation="MKFVVSSTELLSHLSVVSRVISSKNTLPILDNFLFNLEGNELEI
                     TASDLETTLVTRFSLENVSDEGTVAIPYKILIDTLKEFSEQPLTFDINPGTQNITINS
                     ENGQFHIVGQPADDFPQRAMLKPEDSHSVKLSAEILLNGINKTIFATADDEMRPMMNG
                     IFTELNEQGITFVSSDAHRLVRYRRKDVQVEADASFILPKKPAQLLKNMLVKETGFVN
                     VQFDDKNAVFTMPGYTMVCRLVEGNYPNYNAVIPVDNPNRMIVDRVDLYNSVRRVSIY
                     SNAASNMVKLSLTGNQCTVSAEDLDFSVSAFERLSCEYEGTEMEIGFKSIFLSEILAN
                     ISSTEVVLSFSEPSLASLVFPHINDNPDEDILMLLMPMMIGD"
     gene            6506..7273
                     /locus_tag="PK502_07535"
     CDS             6506..7273
                     /locus_tag="PK502_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPH00706.1"
                     /translation="MKLNIKNPLAVIDLETTGIDIVHDRVVEIAVLKILPDGSENMRV
                     RRVNPGMPIPPEATAIHGITDEDVRNEPTFKEIARSLANHLEGCDFAGFNSNKFDLPL
                     LAEEFLRAGVDIDLKRRKFIDVQTIFHKMEKRTLGAAYKFYMDKELTDAHTAGADTMA
                     TYEILMAQLDRYPELKNDVNYLSEFSSFNRNVDFAGRIILDDRGVEVFNFGKHKGLPV
                     VEVLKKEPSYYSWMMNGDFPLYTKRVLTAIYLKMKQQ"
     gene            complement(7296..8243)
                     /locus_tag="PK502_07540"
     CDS             complement(7296..8243)
                     /locus_tag="PK502_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HPH00707.1"
                     /translation="MERLTEVEKEKILFIINPISGIRPKGNVEKNILKYLDNVKYRAE
                     FHVTTCKGDATAAVQAKLAEGYTKFVAVGGDGTVNEVASAIVGTEATLGIIPTGSGNG
                     LARHLRIPLNLKKAMAIINCGAADRIDYGMVNKIPFFCTCGVGFDAQIGYRFASNGKR
                     GFFNYIKIVISDFLRYKSKKYKLKIDGDGKFKIRAFLITVANSSQYGNNAYIAPLADI
                     RDGKLNLSIISPFKIFEAPVIGIRLFTKRIDKSTMEHSGAISEVVIKRKHKDVVHYDG
                     EPIIMGKKLKISVVPRGLRVIVPATKERIKHPFASRHEP"
     gene            8408..10552
                     /locus_tag="PK502_07545"
     CDS             8408..10552
                     /locus_tag="PK502_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPH00708.1"
                     /translation="MSTTDSSRRVELISNRMGIKPRQVFNVIHLLEEGATIPFISRYR
                     KEATGNLDEVQIAEINSLSAKLLDLDRRKETILTTIEEQGKLTDSLRERIAGCYELSE
                     LEDIYLPYKPKRRTRATIAREKGLEPLALLVFNQVEKDILTKAQTFISDKVANVEEAL
                     QGARDIAAEMLSEDERVRNTVRRNFKQKAIIRSRVVKGKEAEGIKYSDYFEFEEPVRR
                     CASHRFLAIKRGEDEGFLRVSITPPADGVLDELRKSLLKSTGETAKQLELALADSYKR
                     LIEPSIENEFTAELKEKADAEAIRVFAENLRQLLLSPPLGQKNVLAIDPGYRTGCKMV
                     CLDAQGNLIHNETIYPHQPRNEQGKAMSKISSLVETFKIDAIAIGNGTAGRETESLVK
                     RIHFNRDVKVFVVSEDGASVYSASPVAREEFPDYDVTVRGAVSIGRRLMDPLAELVKI
                     DPKSIGVGQYQHDVDQSKLKEGLDRVVESCVNHVGVNLNTASMHLLTYVSGLGPQLAK
                     NIVDYRKQNGPFRSRTELKNVPRLGGKAFEQCAGFLRITEAQNPLDNSAVHPESYHIV
                     EQMAGDLECTVKDLIGNDQLRSRIRVEKYVSEKTGLPTLNDIMLELAKPGRDPRSTIK
                     VFEFAKDIFKIDDLTPGMVVPGIVTNITNFGAFVDIGVKQDGLIHISQLAGRFVSNPL
                     DVVKLHQHVRVRVLEVDIPRKRIQLSLKDVEQ"
BASE COUNT         2885 a         2437 c         2540 g         3053 t
ORIGIN      
        1 tgctgaatct tcgcactatt tccggcaaca tgaggctttg taaggatgag attcttgcct
       61 ttaagcccga tgtgcttata ctggttgatt accccgggtt taaccttcgg atagcacgct
      121 ttgcaaaaaa gaacgaaata cgggtattct actacattgc ccccaaggtg tgggcatgga
      181 acgaatcgcg cgttaaagtt ctaaaaaaat acgtagatac gctcttcatt atttttcctt
      241 ttgagcagga gtattttgcc agacacggaa taaatgcagt ttacctgggc aacccaacac
      301 ccgatgccat tgagcagaaa attggcggta aggttgtaac cttcaaccag tttgttgagg
      361 ataataagct atctcaaaag cccataattg ccctacttgc cggaagtcgt aagcaggaaa
      421 taaggtataa cctaccggtt atgctcagaa tggtggagaa gtttcccgac taccagcttg
      481 tgatagccgg agcaccctcg ttaagcgagg atgtatatcg gccctacgtt gcaggaaaaa
      541 acgtagccat tgttttcgac cagacctacc agctgttaag ctgctctacc ctggcgctgg
      601 ttacttcggg tactgcaacc ctcgaaaccg ctctgctcaa cgtgcctcag gtggtttgct
      661 accggggcaa cattctttcg atgcttgtag cctgggtggt tattcgggta aagtacattt
      721 cgttggttaa cctgaacatg caggctgagg tagtaaagga gcttaagcag ttcgatttaa
      781 ctcccgaaaa tctttacgcc gaagctagca agctgctgcc gggtagcccc gaaagggaaa
      841 aaatgctgac cagctatgct cagcttcaaa agcaaatggg gggtagtggc gcttccatgc
      901 gggttggtga caagatagtt gagcttattt cacgctgatt ttattgctca tcttgttcat
      961 tgatagcaaa tctcccccct acagcaattc tttaacagaa tttagttgtt gccagcgcgc
     1021 tgtttttgta agcaggtgaa tcaatatttt acgttttacc tatattgcac cccaatttgg
     1081 gaaatgaaat agtacatgct acttaaagcc ttaggttgca ttaacagata cggatgtaag
     1141 tcaaatttca acagtatttt atgcaaaagc taacttttac gtttatagta tcgttgatcc
     1201 ttacgttggg tgctgcagga caaaagatta gcattagcct gttcaacaac cttaagctat
     1261 caaccatttt gattacgcca accagcggca gctaccgctt aattctgggt gataatgagg
     1321 ttgaactaaa ggtgaaccaa atactttaca ttagtcgttc tggagattcc ctcatggtaa
     1381 gggatgctaa ccaaaacatg ggaacatgga agagggttac cgtggttggc caaaccggtg
     1441 aggacattat tcgagtaaaa ccaattgtac ctgcaagtcc ggctcgtttg tacgacgata
     1501 acctgagttt ctatgttgac ctcgaccgta tttttgccat caacctaatt gaccaggaaa
     1561 agtatgtggc agctgttgta gaagctgaag gaggacttaa tcgggaaccg gagttttata
     1621 aggttcaggc gttaattgtt cgaacttaca cctttgccca cctaaataaa cacctggccg
     1681 agggttttaa cctatgcgat gaagttcact gccagtcata cctaggtcga agcgagaaga
     1741 atagtgatat tctggatgcc gtacttgata ctaaggatat ggtaattgtt gacgccgaaa
     1801 ggaatccaat tactgctgca ttccatgcta attgtggagg acaaactgcc aactccgagg
     1861 acgtatgggt aaaacccttg ccatacctgg tttctgtgtc cgacaggtac tgcctgggtt
     1921 cgaatagctc aacatgggag cgcagcattc ccatcgggga gtggaagaag ttcctttcac
     1981 aaaatggggt ggacaccctt aaaattaaaa agaattccga tttggatttt caggccaaaa
     2041 cccgaccaaa atactatgct gtcatggggg ttgaaattcc taccactgac attcgtaagc
     2101 atttcaggtt gcgttcggca tggttttcgg tttcagctgg taagagcgag attcgccttt
     2161 cgggtcgtgg ctacgggcat ggggttggcc tttgccagga tggtgctata aatatggctc
     2221 aaaagggttg gacttacgat cagattgtcc aatactacta taagggtgtt agcattgtaa
     2281 agcttacgga gcttgatatg gataggataa gggttgacac aacccttaac aatatggtgg
     2341 agcccaacct accaccccct ggaatggaat ttccacaacg acctcccgac aggggaacca
     2401 ggtagtgttt gattaaattt tataaaaaaa ccaaactacc atgtttgcta tttttaccag
     2461 ggaacttcag aactttttct cctcgctgtc gggctacatt gccgcaatgg tttttttggt
     2521 ggttacaggc ttaatccttt gggtgtttcc gggcaaccta aacattcttg atggtggtta
     2581 tgcctcgcta aacggatatt tttccatagc tccctgggta ctactgctgc ttgttccggc
     2641 tgctaccatg cgaactttct ccgatgagaa gcgttttgga actttggaat taattctcac
     2701 ccgtccaatt tccgacggcc ggattgtttt aggtaaattc tttgccacgc tggtgctggt
     2761 cctgattgtt ctggttccat cgctggttta ctacctttcg gtttacctgc tttcaagccc
     2821 tattggaaac attgatgccg gtggaacctg gggatcattc atcggactct ttcttctggc
     2881 ctcaacctac gcttcggttg gtatttttac ctcaagcctc accgacaatg ccattgtttc
     2941 attcctgttg tccgtgctat tttgcctttt cctattttcg ggcttaagcg ctcttgcttc
     3001 gctgcctctt ttttcaggag taaaaccttt gcttatgttc cttagcatca gcgagcatta
     3061 tgcttctatt agccgaggag tactcgatag tagggatttg gtgtacttca tctcgatgat
     3121 ttttctattt atcattttta cccgtaccgt tttggaatcg agaaaatggt aggggaatgc
     3181 aaaatggatt gctaaaatgg agttgaagaa aagttataag gtaaaaagtt tgctgcggtt
     3241 actggtatcg gttgtcgctg ttgcagctct ttatgcttta tcaagcgttt tgcatttccg
     3301 catcgattta acctccgaca agctttacac ccttcagcct atcaccaggg aaattctcaa
     3361 aaatttgaat ggtgaggttt ccatgaaaat ttatctcgat ggggatttac caaccggctt
     3421 tatccggatg aagaggatgt tgaccgaaac gttggatgag ttcaatgttg cctccagggg
     3481 taaggttcgc tacgagctgg ttaaccctta cagtgcaaaa aactccaggg agcaggatag
     3541 aattttcgcc ggtctgtacg ataaagggct ggcaccaacc agcgttcagc aacgaaacag
     3601 gcagggggga agcagccaaa cgattgtatt ccccggattg cttatgagct acaggggtaa
     3661 ggagatggcc gttaacctgt tgaagaacaa ccctatgctt tcggctgagg agaatttaaa
     3721 cctttctatt cagaattttg aatttgctct ggttgatgcc ctcgtgaagc tttcaactga
     3781 tagccttccg cgaatagcat ttattcaggg gcacggagag ctagatgagt ttgaaacagg
     3841 cgatattgaa aaatcgctat cggagtactt tagggttgat agggtgattc taaatggcga
     3901 tatggaagcg cttaagccct acagcgtagc tattgttgcc ggacccacga agccttttcc
     3961 cgaagaagat aagcttgtgg ttgaccagtt catcatgcgg ggtggaaaag tgctatggtt
     4021 tgttgacccg gttgctgtta gcctggacag cctttcaaca ggtgcatcaa ccctggcctt
     4081 tgttgcccaa accaacctcg atgatatgct gtttcgttat ggcgtgaggt taaacccctt
     4141 catcgttcag gatttgcagt gtgctgtaat tcctgtgaac attgctccat ccggtcaggc
     4201 ttccaagttt gcccctgcgc catggcccta ctttccgcta ctaattccac cgtcaaacca
     4261 cccggttacg cggaatctga accccatact cacccgattt gtaagtcccg tagatacggt
     4321 tggagagaat cctttggtaa aaaaacacta cctgctctac acctctgcta caagccgaat
     4381 tctccaggtt ccccttttaa tcaacctttc agagattgag cagacaccaa acgagcagca
     4441 ttttataaac cctaacgttc ccgtagccgc cgagcttgaa ggatacttta cctcggtttt
     4501 taccaaccgt ccgttgaaga attacaacca tggtgaacct tttgagtttc gtgctaaaag
     4561 ctcgtttacc cgaatggtgg tagttgccga tgccgacatg gttagaaacg atgtgcagca
     4621 ccgccctaat ggagcctatg tgcttccgtt gggatacgac cgttacaccc agcaaacata
     4681 cggaaacaag gagctggtga tgaacatggt aaagtacctg aatgatgata atgggatagt
     4741 taaccttcgc tcacgcgact tcaagctcag gctgcttgac aggagcaagg cttcccaatc
     4801 gcgcttggca tggcaagttg ttaacaccgt tttgccatcg ctagtgctgg tgctaggtgg
     4861 cctaatctat ttttttatcc gaagaaatca ttattctacc ccaaagtaat gccaacccta
     4921 taacgtagtt agggcgcggc taatatccgt ccctacttct aaaatgtggc aaaccattag
     4981 aataagccac atttcaagaa aaaatattaa ataggtaggc tttttaaact ttaaacggac
     5041 aaaaattcgt aatattgaac ctgaaaattt aagtagatca ttctggtaat cagtaatttt
     5101 taaattttgg cgcctaactt ctttaagtat ggcaagggtg tttttactct caaaagataa
     5161 gtaaaagtag cattgataat aacttaaaac ttacgaagaa tgaaatttgt agtatcaagc
     5221 actgagctat taagtcacct ttcggtggta agcagggtga ttagcagcaa gaatacgcta
     5281 ccgattctgg acaactttct gttcaacctt gaggggaacg agttggaaat tactgcgtcc
     5341 gatttggaaa ccacgctggt aacccgcttt tccctcgaaa atgtttccga tgagggcact
     5401 gtggctattc cctacaaaat tctgatagat acgctaaaag agttttccga acaaccgttg
     5461 acttttgata taaatcccgg tacccaaaac atcaccatta actctgaaaa cggtcaattt
     5521 cacattgttg gacagcctgc cgatgatttt cctcaaaggg caatgctgaa acctgaagat
     5581 tcgcatagcg ttaagctttc tgccgaaatt ctactcaatg gcattaacaa aaccatattt
     5641 gccactgccg atgacgaaat gcggcctatg atgaacggca tatttaccga gctcaacgaa
     5701 cagggaataa ccttcgtatc atccgatgca catcgcttgg ttcgctaccg cagaaaggac
     5761 gttcaggttg aagcggatgc ctcgttcatt ctacccaaga aacctgccca gctactcaag
     5821 aatatgctgg taaaggaaac cggttttgtt aacgtgcagt tcgatgataa aaatgcggtg
     5881 tttaccatgc ctggctacac catggtttgc cgcctggtcg aaggtaacta tcccaactat
     5941 aatgccgtta tccccgttga caaccctaat agaatgatag ttgacagggt tgacctctac
     6001 aactcggttc gccgggtctc catctactcc aacgctgcca gcaacatggt taaacttagc
     6061 ctcaccggaa accagtgcac cgtttcggcc gaagacctcg atttttctgt ttcggcattc
     6121 gaaaggctaa gctgtgagta cgagggaacc gaaatggaaa ttggatttaa gtcaattttc
     6181 ctatccgaaa tattggccaa catttcctct accgaggtgg ttctttcttt ctcagaacca
     6241 tctcttgcct cacttgtttt tccccacatc aacgataatc ccgacgagga tatattgatg
     6301 ctgcttatgc caatgatgat tggcgattaa gctagagctt accaggcacc ccggagcttt
     6361 taattccggg gaattttttc gggttgcatg gtaggcttgt cgcatattcg acccacttaa
     6421 gggtttgatt tacctttaat gcaaggttaa ccatttatat ttgattaaga ttagcgttta
     6481 ttgcaccatt tcaacagcta ttgccatgaa gcttaatata aagaatccgc tcgccgttat
     6541 tgatttggaa acaacaggaa ttgatatagt gcacgacagg gtggttgaga ttgcagttct
     6601 taaaatttta cccgatggct cggagaatat gagggttcgt cgggtgaacc ccggaatgcc
     6661 tattccgccc gaggctactg ccattcatgg cattaccgat gaagatgtaa ggaatgaacc
     6721 aacatttaag gaaatagctc gctcgctggc taaccatctg gagggttgcg attttgcagg
     6781 attcaactct aacaagttcg atcttccact gctggccgag gagttcctgc gtgctggtgt
     6841 tgatatcgac ctcaagcgtc gaaagtttat tgatgtgcaa accattttcc acaagatgga
     6901 aaagcgcacc ctgggcgcag cctataagtt ttacatggat aaggagttaa ctgatgccca
     6961 taccgccgga gccgacacca tggctaccta cgaaatactc atggctcagc ttgaccgtta
     7021 ccccgagctt aaaaacgacg tgaactacct gtccgagttc agctcgttta accgcaatgt
     7081 tgatttcgcc ggccgaatca ttttggatga cagaggagtt gaagtattca actttggtaa
     7141 gcacaaaggg ttgcctgttg ttgaggtgtt aaaaaaggag ccaagctact actcgtggat
     7201 gatgaacggc gatttccccc tttacactaa aagggtactt acggccattt acctgaagat
     7261 gaagcagcag tgatagtgta aagggctaat atcccttagg gttcatggcg tgaggcgaat
     7321 ggatgtttaa tacgttcttt agttgcagga actattaccc ttagcccacg aggtactacg
     7381 cttattttca acttcttgcc cattattatt ggctcaccat cgtaatgcac cacatcctta
     7441 tgcttacgct taattaccac ctctgaaatg gcaccgctgt gctccatagt acttttatct
     7501 atccgctttg taaagagcct tatgccgata accggagcct caaaaatctt gaaaggggag
     7561 atgatgctta ggtttagctt cccgtctctt atgtctgcca agggtgcaat gtaggcatta
     7621 tttccgtact gtgatgagtt ggcaacagtg ataaggaatg ccctaatctt aaacttccca
     7681 tcaccatcaa tctttagctt atactttttc gatttgtagc gtagaaaatc ggagataaca
     7741 atttttatgt agttgaagaa tccgcgtttg ccattactgg cgaacctgta gcctatttga
     7801 gcatcaaacc caaccccgca ggtgcagaag aatggaattt tattcaccat cccgtagtca
     7861 atcctatcag ctgctccgca gttgatgata gccattgctt ttttcaagtt caacgggatt
     7921 ctcaaatgac gggcaagccc gtttccagaa cccgttggaa ttatgcccag tgtagcctcg
     7981 gtgccaacaa ttgcgctggc tacctcgttc actgttccgt cgcccccaac agcaacgaat
     8041 ttcgtatatc cttcggcaag tttagcttga acagccgccg ttgcgtctcc tttgcatgtg
     8101 gtaacatgaa actcagcccg gtattttaca ttgtcgagat acttgagtat attcttttcc
     8161 acgttgccct tgggccttat gccggaaatc ggattgatga taaataaaat tttttccttt
     8221 tctacctccg ttaaacgctc catgggtttg ttgtttgctg ggttgttgcg gttggtgcaa
     8281 atgtaagttc aaaagaggaa tagtgcaaca gatgatgatt ttgcatggtt tcattggctt
     8341 catttttatt gctgctcttg cctcacacaa ataattttac tttttttgtc ggctaattag
     8401 ttttcctatg agcacaaccg atagcagcag gcgagtagag ctgatttcta acaggatggg
     8461 tataaagccc aggcaggttt ttaacgttat ccacctgctg gaggagggcg caaccattcc
     8521 gtttattagc cgctaccgta aggaagcgac aggaaacctc gacgaagttc aaattgccga
     8581 aattaactcc ctctctgcta agcttctcga tctcgacagg cgtaaggaaa caatcctgac
     8641 caccattgag gagcaaggaa aattgaccga cagcctcagg gagcgtattg ctggatgtta
     8701 cgagttgtct gagcttgagg acatctatct gccctataaa cctaaacgca gaacccgtgc
     8761 aactattgcc agggagaagg gtctggaacc gcttgccttg ctggtgttta atcaggttga
     8821 aaaggatatt ttaacaaaag cccaaacctt tatatccgat aaggttgcca atgttgagga
     8881 ggcgttacag ggtgcgcgcg atatagctgc cgaaatgcta agcgaggatg aaagagttag
     8941 aaatactgta cgccgcaatt ttaagcagaa agccattatt cgttcaagag ttgtcaaggg
     9001 gaaggaagcc gaaggcataa aatattccga ttactttgag tttgaagagc ctgtccgacg
     9061 ctgcgcttct cacaggtttt tggctattaa acgaggcgaa gatgagggtt ttctccgggt
     9121 ttccataacc cctcctgccg atggcgttct tgatgagcta cggaaatcgc tgctgaagtc
     9181 aaccggtgaa acagccaagc agctcgaact tgcactagcc gattcctata aaaggttgat
     9241 agaaccatca attgaaaatg agtttactgc cgagctaaag gaaaaggcgg atgctgaggc
     9301 aatacgagta tttgccgaaa accttcgcca gctactgctt tcccctccgt tgggacagaa
     9361 aaatgtgctg gctattgatc cgggctaccg tacgggctgc aagatggttt gccttgatgc
     9421 tcagggaaac cttatccata acgaaaccat ttatccacat caaccccgca acgagcaggg
     9481 aaaggctatg tcaaaaattt caagccttgt tgaaacattc aaaattgatg caatagccat
     9541 tggtaacgga accgccggtc gcgaaacaga gtcgcttgta aagcgtattc atttcaaccg
     9601 agatgttaag gtctttgtgg tcagcgagga tggcgcatct gtttactccg cttctccagt
     9661 tgcccgtgag gaatttcccg actatgatgt aactgttcgg ggtgcagttt ccattggtcg
     9721 taggctgatg gacccgcttg ccgagctggt taaaatcgat ccaaaatcaa ttggggtagg
     9781 gcagtaccag cacgatgttg accagtcaaa gcttaaagag gggcttgaca gggttgttga
     9841 gagttgtgtt aaccatgtag gggttaacct gaatactgcc agcatgcatt tgctaaccta
     9901 tgtctctggc ttaggacccc agctggccaa aaacattgtt gattacagga agcagaatgg
     9961 cccattcaga agccgaacgg agctgaagaa tgttccccgg cttggtggaa aggcatttga
    10021 acaatgcgca ggctttttga gaattaccga agcccaaaac ccacttgaca acagcgccgt
    10081 tcatcccgag agctaccata ttgttgagca aatggccggt gatttggagt gcacggtgaa
    10141 agacttaatt ggcaacgacc agctaagatc tcgaattagg gttgagaagt atgtttcaga
    10201 gaaaacgggg cttcctaccc tgaacgacat aatgctggaa ctcgcaaaac ccggtcgcga
    10261 ccctcgtagc actattaaag tatttgagtt tgccaaggac atttttaaga ttgacgacct
    10321 aacccccggc atggtggttc cgggaattgt taccaacatt accaattttg gtgcttttgt
    10381 tgatataggt gttaagcagg atggacttat ccacatctca cagctggccg gtaggtttgt
    10441 ttcaaacccc ctcgacgtgg tgaagctgca tcagcatgtg cgggtaaggg tgcttgaggt
    10501 tgatattccc cgaaaaagaa ttcagctctc gctgaaggat gttgagcaat agtctattac
    10561 ggaggcggat agaattgaac cctgagcagt tagacaggtg ttatggtgag tagttcgact
    10621 ggctcactat aacaccagcc gaactactca ctgtaacacc agtcgaagga gcctgccctg
    10681 agtttgtcga agggtgaatc cttctaaaac cggacaaatc cactttatac tttcctcttt
    10741 ctttagcccc aacggggcgg aataggtagc aaggggcgca gccccttgtg gcgtggcggt
    10801 atgatatatt aagccctgaa ggggcggaat atttaaattt gtttttcccg ttgcgatgca
    10861 aagggtcaaa attatgcttt ggagttaaac ccaaggcttt gccctgggct aggct
//
LOCUS       DAOPHX010000090        10869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_7384_length_10869_cov_5.652857, whole genome shotgun sequence.
ACCESSION   DAOPHX010000090 DAOPHX010000000
VERSION     DAOPHX010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 10869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10869
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7384_length_10869_cov_5.652857"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..7357
                     /locus_tag="PK502_07550"
     CDS             323..7357
                     /locus_tag="PK502_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPH00709.1"
                     /translation="MNNLKRIFLAGLTLVGSAASGQIYFTEGFENGTRPSGWSEIYVS
                     GSQVWLYRNGGHSPNDPSWSIPASQEDITRNPSSAHSGTYNAIFFKQSSAHEQTKLVT
                     KAIDLSEGIKPELSFWLCQVPWTFGGSTNNDELRVYYKNTFGGAWTLLAEYTDPIFDW
                     TEYKINLPNPSSTYYIAFEGKINWGFGTCIDDIVIEEKGYQERYVSELTVSQADFSFI
                     PSGSPNEPILKVGVKVFGNMGSATLNTLTAKSLNTSDNDIEANGVKLYFTPTGTFSAD
                     NLLVEGKNFTSGQVTFNNINFNLPNGQSYLWLTYDVKQNAVHGNIMDARLDAGSIQVS
                     DSLYPKVNESPEGNRVIYETIYRQDFEGTHQWLLSGEFQVDVPQGKGGAYIGFPDPTT
                     AYSGSKVLGTDLTGLGATQGDYENYVTEATTYKAISPIIDALFFKDLKISYRRWLNIE
                     VWDRASVEVSKDNGTSWSSIWHNNNYFSDNLWLKTAHDVPPTISRSNQLKFNFKLGPT
                     DGATTYSGWNVDDFIVTGDYITKDVGVVEWVYPLSGCGHSASDSVTVKIANLGALASP
                     TPIPVQYSFNNGTTWTTNYYTGSIPAGDTVTFTFSTRVNLSTPGIKYVKARTRLNGDE
                     DASNDELSAQVYIVPTYLLPYTENFESNDGYWRSYGPSLWQWGSISKPAMSGGSKSWA
                     TSLSQNYGTALTGAPDTLFEDNFEIPDGWTLTGEFERNTIIMGWDTIPTFAYSGIYCL
                     GTDLSRQGAHKGMYEPNSSYEALSPAVDISSYANLQLAYYRWFKVMEGDTAKVMASNN
                     GLTWTTIWNSAGQEIADENWTEEIIDIPASLVNGSSLTIKFVLNANGDENIAEGINID
                     DFRIIGNHFSTGMAYLQSPCFNFTGISIPVMDMSIFNRTEAGVDGTTLYYSTDKGETW
                     TPVDNNDTLDDYWNFYTDSLVSSIGLDGWNGLGSGWENTRHLLPAALANNSSVIFRLG
                     FKADKTNNDFGGTAIDNFKLLQAPFDVGVESILSPSTTCDLSQNQSIQVRLKNFGIQD
                     MEAGDTIIVGIDIDHPMVPDSFTDTLVLSSTLAVNSHLDYTISKTFNMSVSGDYQVTA
                     FTKIENNPVFYHPEPNDTASTLITVQKPYVELGNDIYSYNPENIVLDATNPDPTVTYK
                     WYKSPDLTTVIGTNPTYAIADHDGGKYLVTLTNSIPCSSTDSVTVNRIIRDVGISTLV
                     SPTSSCELSDETPLSVYVKNYGSDTLMSSDDEIYLHYTFNGNPQVDTTWTITQNLLPG
                     DSLLFTFNQTFDMQAVGSYPLHVWANTTLDENAANDGPNTTIDVWGYPTFSLYQTMGL
                     PKDSVSVTLTSYTLDAGPTWDGYLWHNDSSTLQTYDMIETGWGVVTVQDNHGCPATDS
                     VYVDLQFSDVSVDEVVSPLTSCEIPGTTYPHIKIRNSGTNPISAGTDITLKYRLNGDL
                     KESSILTLPSSWNANEVMDVEFSVGVDISAPGTYSLDFIATTTTDDKPENDSIRHEIT
                     VFGTPSLELGDSVFTRNTSYNLDAGSGHESYSWSTGETTQQISVTQTNTYWVEVVDDG
                     ICSVRDTVVVTFLRHDYSITEIVNPVTSCSTPIPQPVSVKLTNTGNDTLKSGQSIILG
                     YQIDDEYFAYENHTLLSNLNPGQFLLYTFADPVDLSAPANLQVLAYSYFPDDANASND
                     TLLVSIEIMESPTIDLGEDRVDRTGSITLDGGEGADYSYLWQDNSTNRFLIVENTGDY
                     WVTTTAPNGCFDQDTVYLTMLKPDYRVSAILSPSSACSLSNAESIQVEIENLGTDTIL
                     VGQNIFVSYEVNSILQQTQSITMTSKFKPGDKVYHTFSKTYNMANPGSYIIKAYTSFS
                     GDLNPFNNATTAPITNYGPPQVNLGDDLAFCDGSSAILDAGPGMASYLWNTGAISQTI
                     NVSTPGEYSVEVTDSHGCTNSDNIHITVNGLPTVTHLPLDPVCLDAEAFTLTGGNPAG
                     GSYTGSGVSGGTTFTPSGAGVGTHTLTYLYTDGNGCSNSTNVGITVNALPVVNLGSNQ
                     SIEEPLTLDAGAGFAAYQWQDGSTSQTYTVEATGLYSVTVTDGNGCQAYDEVYITFLE
                     TLDVIVSDLISPTDKCYDEVDEPVTVELTNRGTKTFTTGEQLNVSYQVGSAQPVTETL
                     TFSGSFAQNDKLQFTFSNNLALSTGNHTFKCYTSIAGEDGEIISYNVMVYNLPNLNLG
                     ADTIRHSLPYVLQAGVEAQTYLWSTGATGTASITVSQWGKYWLTITDSHGCVASDTTV
                     IWWPIGVEELPGTNDKVYIFPNPADKKLNIRIDGENEATFTIDLLNHQGIRVNSAKLQ
                     VPSELSTTFDVEGLAPGIYFLHVYSSKNSTVFKVVVKR"
     gene            7713..>10869
                     /locus_tag="PK502_07555"
     CDS             7713..>10869
                     /locus_tag="PK502_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPH00710.1"
                     /translation="MYKIQTHPILEVPKTESITFLFNGNEVKGAKGFTIAAALHQAGY
                     PVHSHSLENRERSLECGIGKCGACEMLVDGQIKRICITPVDGVKEVSEIPKDYAPGVK
                     PADKLHSTRVYKTTVAIIGAGPAGLAARELLLQHGIENIVIDNNATIGGQFNMQTHQF
                     FFFEKEKKFGGMRGFEIAKTLAGDNAEGIFLNSTVWDILENKRIAVKNIRTDEIYFVE
                     ADHLVVATGAVPFMPTFENDDLPGVYTAAVVQKMMNQEFTLLGKNVLTVGAGNIGYLT
                     SYQLMQAGAKVKAIIEAMPNEGGFPVQANRVRRLGIPIMLGKTLVKAVPNESHTGIVG
                     AIIADAVNFKPVPGTEQLLEGIDAINICTGLVSDDQLIIKGREVFGRNCHGAGDAIRI
                     GEGTSAVLRGKQVAYEILQELDVRFNYDNFLDISKEYIDSQQHPVRVIDKPFIPDEER
                     RRSKPFVQIDCLYGFACNPCTFACPQGAITKSSTSAVPQIDYTKCTGCFDCVYQCPGL
                     AIFGYSYTKDWLFLPIEYEAQEGAEVFLVDNNGKKLGEGIIEKILRKPNKTNVARVKS
                     LSLHGDDLLNARGFIVKSSYPQPLDTKPVSHKSEEKIYVCHCDDVTLDEVLETIGDRK
                     FISADEIKHTTRLGMGACRGKRCIKRLKTTLAPLGIQIVGDATPRGPLSNQLTLGELY
                     PKDVHETYITGVNGKTVRVEKVSALVAGGGIGGSALFRYMAEAGLKPILLNFGRGSSW
                     RNIAGGRTAFSLPEIADIAKVNHEIFRELHGISSIDYRPINYVNFAHDDSTLKALEVS
                     MAWSDAYMVEPKDFSKEISPYMNPSLATYKAAQITRGCWQATPGKTVDLLRKIGIRNG
                     GTVKEDCELISVYRDGAGYVALVRDHDHSYIEFHADSFVNALGPSGEKFNRQLGIYTG
                     LYPVKHQAFITRRLPWLGINNAPLGMLIDRRNYKGFVAVYGQQLAETGQIIGCASPAV
                     DAREADKNLKVNSQEFLEIASEVFSSWIPMLGTVGFQAVWAGYYTEPRMFIDPKHGLF
                     TGLRGQGFMLSMGLAKMYVDAL"
BASE COUNT         2967 a         2803 c         2524 g         2575 t
ORIGIN      
        1 atacaagggg ctgcgcccct tgctacttat tccgcccctt cggggcttaa aacactagac
       61 aagttccgcc ggtagtcgag caagttccga aatccgctgg taggcggaca agttctggaa
      121 tctaaaatcc gccagtagtc ggaaaaattc ttgaatcctg cttgattttt tttatccttt
      181 atcctttacc ctttaacctt tttggatatt ttatcgtaaa ataatattaa tttgcattag
      241 taaactacct cttaccctaa cttaataatc atctattaag ctgataaata gatacgctaa
      301 aaccataaga aaatataccc taatgaataa cctaaaaaga atatttctgg cgggcttaac
      361 tcttgtaggt tcggctgcca gcggacagat atattttacc gagggatttg aaaacggtac
      421 caggccttct ggttggagcg agatttacgt ttcgggaagt caggtatggc tgtaccgcaa
      481 tgggggtcac agccctaacg atcccagctg gagcattcca gcctcccagg aggacatcac
      541 ccgcaaccct tcttcggccc actcgggtac ctacaacgcc atattcttca aacaaagttc
      601 tgctcacgag cagaccaagc tggttactaa ggcaatagac ctaagcgagg ggataaaacc
      661 agaactcagc ttctggcttt gccaagttcc ttggactttt gggggtagca ccaacaacga
      721 tgagctgagg gtgtactaca agaatacgtt tggtggagcc tggacattgc tggccgaata
      781 caccgatccc attttcgatt ggacagagta taagattaac cttccaaacc cctcgtcaac
      841 ctactacata gcctttgagg gtaaaatcaa ctggggcttt ggaacctgta ttgacgacat
      901 tgttattgag gaaaaaggct accaggaaag gtatgtgagc gagcttactg ttagccaggc
      961 cgatttttca ttcattccaa gcggttcccc caacgagccc atcttaaagg ttggggttaa
     1021 ggtttttggt aatatgggta gcgcaacact caacacccta acggcaaagt cgctaaacac
     1081 cagcgataac gatattgagg ccaatggagt aaaactctac ttcaccccaa ccggaacatt
     1141 tagcgctgac aacttgctgg ttgaggggaa aaattttacc tcaggccagg ttactttcaa
     1201 caatatcaac ttcaacctac ccaacgggca gtcgtaccta tggctaacct acgatgtgaa
     1261 gcagaatgct gttcatggga atataatgga tgccaggctg gacgccggaa gcatacaggt
     1321 tagcgactcg ctttacccaa aggtgaacga atcgcccgaa ggtaaccggg tgatatacga
     1381 aacaatttac cgtcaggact ttgaagggac acaccagtgg ttgctgagcg gagagtttca
     1441 ggtggatgtt ccccaaggaa agggaggagc atatattggt tttccagacc caaccacagc
     1501 ctacagcgga agcaaggtgt tgggaacgga ccttaccggt cttggcgcta ctcaaggcga
     1561 ctatgagaac tacgttaccg aggcaacaac ctacaaggct atttcaccaa taattgatgc
     1621 cctgttcttc aaggatttaa agatctcata ccgccgctgg ctgaacatag aggtttggga
     1681 tagggcatcg gttgaggtgt cgaaagataa tggaaccagc tggagcagca tctggcataa
     1741 taacaactac ttttccgata acctttggct caaaactgcc cacgatgtac ccccgaccat
     1801 atccaggtcg aaccagctga agttcaactt taagcttggc cccaccgacg gtgcaactac
     1861 ctactcgggt tggaatgtgg atgatttcat cgttacaggc gactatatta ccaaggatgt
     1921 tggggtggtg gagtgggttt accccctttc gggctgcggt cattccgcct cggacagcgt
     1981 tacagttaaa attgccaatc ttggcgcttt agcttcgcca acaccaatac ccgtgcagta
     2041 ctccttcaac aacggtacta catggacaac caactactac accggtagca ttccggcagg
     2101 cgataccgtt acttttacct tcagcacccg ggttaatctt tctacccccg ggattaaata
     2161 cgtaaaagcc cggaccaggc ttaacggcga tgaggatgcc tccaacgatg aactttctgc
     2221 tcaggtttac attgtaccca cctatctcct accttacacc gaaaactttg agagcaacga
     2281 cggctactgg cgttcgtatg gaccctcctt atggcagtgg ggcagcattt ccaaaccggc
     2341 tatgagcggc ggttcaaaat cgtgggcaac cagcctttct caaaactacg ggacagcgct
     2401 caccggtgca cccgacacgc tatttgagga caacttcgaa atccccgacg gctggacgct
     2461 gacgggcgag ttcgaacgga acaccataat catgggttgg gacaccattc caacctttgc
     2521 ttatagcggc atatactgcc tcggaaccga ccttagcaga cagggtgccc acaagggcat
     2581 gtatgaaccc aattccagct acgaggcatt gagcccggct gttgacatca gcagctacgc
     2641 caacctgcag ctggcctact atcgttggtt taaagtaatg gagggcgata cggccaaagt
     2701 tatggccagc aacaacggcc ttacctggac caccatctgg aacagcgccg gacaggaaat
     2761 agccgacgaa aattggaccg aagaaattat tgatataccc gcctcgcttg ttaatggcag
     2821 cagccttacc atcaaattcg tcctcaatgc aaatggcgat gagaatatag cggaggggat
     2881 aaatattgat gactttagaa ttatcggcaa ccatttttca accggaatgg cctacctgca
     2941 aagcccatgc ttcaacttta ccggtatctc catacccgtg atggacatga gtatttttaa
     3001 ccgcactgaa gccggagttg acggcaccac actttactac tccaccgata agggcgaaac
     3061 gtggactcct gttgacaaca acgataccct ggatgattac tggaatttct acaccgatag
     3121 cctggtttca tccatcggcc ttgatggatg gaacgggttg ggaagtggtt gggagaacac
     3181 ccgccacctg cttcctgctg ctctggctaa taatagtagc gtaattttca ggttaggctt
     3241 caaagccgat aaaaccaata acgattttgg aggaactgcc attgataact ttaaactact
     3301 ccaggcacca tttgatgtgg gtgtagaaag catattaagc ccttccacca cctgcgattt
     3361 aagccagaac caatccattc aggttaggtt gaaaaacttc ggcattcagg acatggaggc
     3421 tggcgatacc ataatcgtgg gcattgatat tgaccatccc atggttcccg actccttcac
     3481 cgacaccctg gtgctgagtt ccacgctggc tgtgaacagc cacctggact acaccatttc
     3541 caaaaccttc aacatgtcgg ttagcggcga ttaccaggtt acagctttta ccaagattga
     3601 aaacaaccca gtattctacc acccggagcc caacgatact gcttctactt taattaccgt
     3661 gcagaaaccc tatgttgagc tgggcaacga tatttactcc tacaaccccg aaaacattgt
     3721 gctggatgcc accaaccccg accccaccgt aacctataaa tggtacaaat cgccggacct
     3781 gactacagtt attggtacca atccaaccta cgccatcgcc gaccatgatg gaggaaaata
     3841 ccttgttacg cttacaaatt ctattccctg ctcctcaacc gactcggtta ccgttaaccg
     3901 tatcatacgc gatgtgggga tttcaaccct ggttagccca actagctctt gtgagctttc
     3961 cgatgaaacg cccctgagcg tttacgtaaa gaattacgga tccgatacgc tcatgagtag
     4021 cgacgacgaa atataccttc attacacttt taacggcaac ccccaggtgg ataccacctg
     4081 gaccattacc caaaacctgc ttcccggcga ttcgctacta ttcaccttca accaaacttt
     4141 cgatatgcag gctgttggtt cttacccgtt acatgtttgg gcaaacacca cactagatga
     4201 aaatgccgct aacgatgggc ctaacaccac catagacgta tggggctacc caacctttag
     4261 cctttaccag accatgggtc tgccaaaaga ttcggtcagc gtaaccctaa cctcctacac
     4321 ccttgacgcc ggtccaacat gggatggcta cctgtggcat aacgacagct caaccctcca
     4381 aacctacgac atgatcgaaa cgggatgggg tgtagtgacg gtgcaggaca accacggctg
     4441 ccccgctacc gattccgttt acgttgacct gcaattctcc gatgtttcag tggatgaggt
     4501 ggtttcacca ttaacctcct gcgaaattcc cggtaccacc tatccccata taaaaataag
     4561 gaattccggc accaacccga taagcgcagg aaccgacatt acgctgaagt acaggctcaa
     4621 tggcgatttg aaggaaagca gcattttaac acttccctca agttggaatg caaacgaggt
     4681 aatggatgtg gagttctcgg ttggcgttga catatcggct ccgggaacct actccctcga
     4741 ctttatagca accaccacca ccgacgataa accagaaaac gacagcataa ggcatgagat
     4801 aacggtgttc ggaaccccat cactggagct aggcgattct gtttttaccc gaaacaccag
     4861 ctacaacctt gatgccgggt cgggccatga aagctatagc tggtctaccg gcgaaaccac
     4921 ccagcaaata tccgttacac agaccaacac ctactgggta gaggtggttg atgacggaat
     4981 atgctctgta agagacaccg tggtggttac cttcctccgg cacgattact ccataaccga
     5041 aatagttaac cccgttacat cgtgcagtac gcctattcct caacctgtgt cggttaagct
     5101 taccaacacc ggtaacgata cccttaaatc cggacagagc ataattcttg gttatcagat
     5161 tgacgacgaa tactttgcat atgaaaacca tacactcttg agcaacctta acccagggca
     5221 gttcctgctc tacacctttg ccgaccccgt tgacctgtcg gctcctgcaa acctgcaggt
     5281 gcttgcctac agctacttcc ccgatgacgc caatgcctcc aacgataccc ttttggtttc
     5341 tatcgagatc atggaatcgc ctacaattga tttgggcgaa gacagggttg accgaaccgg
     5401 gagtattacg ctcgacggag gtgagggcgc tgattactcc tacctgtggc aggataactc
     5461 taccaatagg ttccttattg ttgaaaatac cggtgattac tgggttacta ccactgcgcc
     5521 caacggctgc ttcgatcagg ataccgttta cctcaccatg ctgaaacccg actacagggt
     5581 ttcggctata ctttctccct cgtccgcctg ctccctatcc aacgctgaaa gcattcaggt
     5641 tgaaattgaa aatcttggta cagacacgat acttgttggg caaaacatat ttgtttccta
     5701 cgaggtgaat agcatcctgc agcaaaccca gagcataacc atgacttcta agtttaagcc
     5761 cggcgacaaa gtttaccaca ccttttccaa gacttacaac atggcaaatc caggaagtta
     5821 catcattaag gcctacacct cattttcggg cgaccttaac ccctttaaca acgccacaac
     5881 tgcacctata accaactacg ggcccccaca ggttaatctg ggagatgatt tggcattttg
     5941 cgatggctcc tcagccatcc ttgatgccgg acctggcatg gccagctacc tgtggaatac
     6001 cggcgcaatc agccaaacaa tcaacgtatc tacaccaggt gagtactccg ttgaggttac
     6061 cgatagccat gggtgtacca attccgataa cattcacatt acggttaacg ggctaccaac
     6121 ggttacccac ctacctcttg atccggtttg tcttgatgca gaagccttta cccttacagg
     6181 gggtaaccca gccggaggca gctatacggg tagcggggtt agcggcggca caacttttac
     6241 cccatccggt gccggagtag ggacacatac cctgacctac ctctacacgg atggtaatgg
     6301 ttgctccaac tccaccaacg tgggtatcac ggtgaatgcg ctgcctgtgg ttaatttggg
     6361 ttcaaaccag tctattgagg aaccgcttac cctcgatgcc ggggcaggct ttgctgccta
     6421 ccagtggcag gatgggtcaa ccagccaaac ctataccgtg gaggcaaccg ggctgtactc
     6481 ggtaaccgtt accgatggca atggctgcca ggcctacgac gaggtctata tcaccttcct
     6541 agaaaccctg gacgtgattg tttcagacct aatctccccc accgataagt gctacgatga
     6601 ggttgacgag ccggttaccg tggagctcac caacagggga accaaaacct ttaccaccgg
     6661 tgaacagcta aatgttagct accaagttgg ctctgcacag cctgttacag aaacacttac
     6721 attctcgggt agctttgcac agaacgacaa gctgcagttt accttttcaa acaaccttgc
     6781 gctaagtacc ggtaaccata cctttaaatg ctacacctcc attgccgggg aggatggtga
     6841 aataataagc tacaatgtaa tggtttacaa cctacccaac ctcaaccttg gtgcagatac
     6901 catcaggcat tctctcccat acgtgctgca agcaggggtt gaagcgcaaa cctacctctg
     6961 gagtacggga gcaaccggta ccgcctctat taccgtttcg caatggggaa aatactggct
     7021 aacaataaca gatagccatg gttgcgttgc atccgacacc acagtaatat ggtggcctat
     7081 tggcgttgag gaactccctg gaaccaatga taaggtttac atatttccaa acccagcaga
     7141 caaaaaacta aacattagaa ttgacgggga gaatgaagct acctttacca ttgacctcct
     7201 aaatcatcag ggcatcaggg tcaactcggc caaattgcaa gtaccttcgg agcttagcac
     7261 taccttcgac gttgagggat tggctccggg gatttacttc ctgcatgttt actcatctaa
     7321 gaattccact gtgtttaagg ttgttgtgaa aagataacca acaaattcgg taggcaaggt
     7381 gctaaacgct caacccagaa tttcactgaa atggggttgg gcgtttggca gtattatctg
     7441 gtcaataaac aggtctactg aaaatgggca ttcccaaaag cgagcctaca accctttctt
     7501 tccatcacct tctacattga aataaaacat gcaaccactt aattttaaga ttgaggtcaa
     7561 tgagtaaaca acccctctca taagttttac cccacgaatt cacatatttt ttataaaccc
     7621 tgacgaatat cattaacagc catacttggt aattcatact tttgtaatca aattacaaga
     7681 aattctatca tagttcttct aaatggcaag ttatgtataa aattcaaaca caccccatac
     7741 ttgaggttcc aaaaacggaa agcattactt ttctatttaa cggtaatgaa gtaaaggggg
     7801 caaaaggatt taccattgca gcggcgcttc atcaggcagg atatccggtt catagccaca
     7861 gtctcgaaaa tcgggaacgt agcctcgaat gcggtatagg caagtgtgga gcatgcgaga
     7921 tgctggtaga tggtcaaatt aaacgtattt gcattacccc tgttgatggg gttaaggagg
     7981 ttagtgaaat acccaaggac tatgcaccgg gcgtaaaacc tgctgataag ctgcacagca
     8041 ccagggttta taaaaccacc gtggccatca ttggcgccgg gcctgccggc ttggcggcac
     8101 gcgagctgct gcttcaacat ggtattgaga acattgtgat tgataacaac gcaacaattg
     8161 gcggacagtt caacatgcaa acccaccagt tcttcttctt tgaaaaggag aagaagtttg
     8221 gcggaatgcg aggcttcgag attgccaaaa ccctggccgg cgataacgcc gagggtattt
     8281 ttctgaacag caccgtatgg gacattctcg agaacaagcg cattgcggtt aaaaatatcc
     8341 gaacggatga aatctacttt gttgaggccg accacctggt tgtagccaca ggagcagttc
     8401 cgtttatgcc cacttttgag aatgacgatt tacccggtgt ttacacggca gcggttgttc
     8461 agaaaatgat gaaccaggag tttaccctgt tgggaaaaaa tgtgcttacg gttggggccg
     8521 gcaacattgg ctaccttacc agctaccagc tgatgcaagc tggtgctaag gtaaaagcca
     8581 ttattgaggc catgcccaac gaggggggat ttccggttca ggccaaccgt gtccgccgtc
     8641 tcggaatacc cattatgctt ggaaaaacac tggtgaaagc agtgcccaac gagagccaca
     8701 cgggaattgt tggagccatt attgccgatg ctgtaaattt caagcctgtt ccaggcaccg
     8761 agcagctgct cgagggaatt gatgccatta acatttgtac aggtctggtt tccgacgacc
     8821 agcttatcat caagggcagg gaggtgttcg gacgtaactg ccatggcgcc ggcgatgcca
     8881 ttcgcattgg cgagggaaca agcgcagttc ttagaggcaa gcaggtagcc tacgaaatac
     8941 tccaggagct cgatgtaagg ttcaactacg acaacttcct cgacatcagc aaggagtata
     9001 ttgatagcca gcaacaccct gtaagggtaa ttgataaacc tttcatccct gatgaggagc
     9061 ggagaaggag caaacccttt gttcagatag actgcctcta tgggtttgca tgcaacccat
     9121 gcacatttgc ctgcccacag ggagccataa cgaaaagttc aaccagcgct gttccccaaa
     9181 ttgactacac caagtgcacc ggctgcttcg attgcgttta ccagtgcccc ggcctggcaa
     9241 tattcggtta cagctacacc aaggattggc tgttcctgcc catagagtac gaggcccagg
     9301 aaggcgctga ggttttcctg gttgataaca acggcaagaa acttggcgag ggtatcattg
     9361 aaaagattct ccgaaaaccc aacaaaacta acgtggccag ggtgaaatcg ctttcacttc
     9421 acggtgacga cctactcaac gctcggggat ttatagtgaa gagcagctat ccccaacccc
     9481 tcgataccaa accggtttcg cataagtcag aggaaaaaat ctacgtttgc cactgcgatg
     9541 atgttacgct tgacgaggtg ctggaaacta tcggcgaccg aaagtttatt tcagccgatg
     9601 aaattaagca taccacccgc ctgggcatgg gcgcctgccg gggaaaaagg tgtattaaaa
     9661 ggctgaagac aaccctggct ccactgggca ttcagattgt gggcgatgcc acaccccgtg
     9721 ggccgcttag caaccaactc acacttggag agctctaccc aaaggacgtt cacgaaacct
     9781 acatcaccgg agtaaacgga aaaacagtta gggttgaaaa ggtttctgcc ctggttgccg
     9841 gcggaggaat aggaggaagc gccttgttcc gctacatggc cgaggccggg ttaaaaccca
     9901 tcctgcttaa ttttggtaga ggctccagct ggcgaaacat tgccggtgga aggacagcat
     9961 tctcccttcc tgaaattgcc gacattgcca aggtaaacca tgaaatattc agggagctgc
    10021 atggcatttc cagtattgac taccgcccta taaactacgt gaactttgcc cacgacgata
    10081 gcacgctaaa agcactggag gtctccatgg cctggagcga tgcctacatg gtggaaccaa
    10141 aggatttcag caaggaaatt tcaccctaca tgaacccttc gcttgccacc tacaaagccg
    10201 cacagattac ccgtggctgc tggcaagcta cccctggcaa aacggttgac ctactccgta
    10261 agattggaat caggaatgga ggaacggtta aggaggactg cgagctgatt agcgtttacc
    10321 gcgatggagc aggatacgta gctcttgtgc gtgatcacga ccacagctac attgagttcc
    10381 atgccgatag ctttgtgaat gcacttggcc cgtcgggtga aaaattcaac cggcagctgg
    10441 gcatttacac cggcctatat ccggttaagc atcaggcatt cattacccgt aggcttccat
    10501 ggcttggtat aaacaacgca ccactaggaa tgcttataga tagaaggaac tacaagggat
    10561 ttgttgctgt ttatggtcag cagctggccg aaaccggaca gataattggg tgcgcctccc
    10621 cggcagttga tgcccgcgag gctgacaaaa acctgaaggt taactcccag gaatttctgg
    10681 aaattgcctc cgaggtgttc tccagctgga taccgatgtt aggaacagta ggatttcagg
    10741 ctgtttgggc aggttactac accgagccta gaatgttcat tgaccccaaa cacggtcttt
    10801 ttacaggctt acgaggccag ggattcatgc taagcatggg gctagccaaa atgtacgttg
    10861 atgcgctaa
//
LOCUS       DAOPHX010000091        10858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_7397_length_10858_cov_6.926687, whole genome shotgun sequence.
ACCESSION   DAOPHX010000091 DAOPHX010000000
VERSION     DAOPHX010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 10858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10858
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7397_length_10858_cov_6.926687"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..982
                     /locus_tag="PK502_07560"
     CDS             296..982
                     /locus_tag="PK502_07560"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF001186.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPH00711.1"
                     /translation="MQPKRFALVVAAGSGLRMGSDTPKQFMELDGLPVLMRTISVFLT
                     LRDIPEVVLVLPEAQITTWQRLCKKHHFETNLQIVPGGSTRFESVKNGLELLPADDSL
                     VAIHDGVRPLVTHEVVERCYAMAARGVCAIPVVNPVESVMIASGGKFTPFPRNNVLLV
                     QTPQTFRSATIKHCYNQPYSELFTDDASVAAACGVELHTVEGNRENIKITTPADLLTA
                     RVLLQAMKTD"
     gene            1007..2179
                     /locus_tag="PK502_07565"
     CDS             1007..2179
                     /locus_tag="PK502_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00712.1"
                     /translation="MSFVLKEVDNRKTRKDFLNLPVELYRDYPSWIRPLNNDIESVFN
                     PKTNKQFRHGEAIRWVLYSDNKPVGRIAAFIDYEIARNNEQPTGGVGFFECINSPEAA
                     TQLFDTAKEWLQQRGMEAMDGPINFGDRDRWWGLLVEGDYPPNYCMDYHPPYYRELFE
                     GYGFKPYFHQFTYYRPVNPEGLDPIIREKAERIARNPAYHVTIISKRNLKKFAEDFRT
                     IYNNAWGRYTGVKKMSHIHAMALMKTLKPIIDPELMYFAYYNNEPIGFFIMVPDLNQV
                     VRHLNGQFGLVHKLKFLYLLKVKKVCTKAIGLIFGIVSQHQGKGVEGALVTEFANRAL
                     RPDFQYKELELNWIGDFNPTMRRVASQIGGKIRKIHITYRYMFDRTKEVVPPRRVS"
     gene            2182..4290
                     /locus_tag="PK502_07570"
     CDS             2182..4290
                     /locus_tag="PK502_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00713.1"
                     /translation="MDKRNRLVERSDEMRYAKYLILKLAMMKQNAVINPRMPMLKRFL
                     GLLLLTLSGYMVAAQRDIEPRLVFSDTSRFNFTGEWQYLSTDIFLLNGDRFSNLINEL
                     NFPNKGNGKIRRKSKLEEERLEYLFITAKLKNVKFFGENDITYPLYNFQISRDKDNRY
                     QTLVSDNIDHVRIIDNLPLYSAHDYIDAEISVKAITNNDRDQMLSLVATQLQNLSKIK
                     TPTDAVMGIIGEFGSFIEANTRKKEYRFSSTIRLFEQKNFDTRVHSLKLYVLTTSNSK
                     PIEVNTALIQNFMDTVRHGQVGRQTLKELLAYHNYPVIAVVNYKSLYRMEPIGGDEVT
                     FASIETRKLRVENDYRQGLINAETYRQEKDFIAFLSVYANFKSYLDAYNLNYRTGNSD
                     AVSISLFKVMQYYRQLVKTFEEMRFKYRGNNTFANIFRREYESVLGFASLYLDDDHNL
                     KATKELVNSLVKLDTIPMLAAADMEAYIASLRFSGAFKPDLMRQTPEGQLITNHLASL
                     EEQLYRSTYEDEVRKLNDVEASVSTRNATEKLLAMIKNTSCGLCRERAIAAINSFGQR
                     MDEFTRKQKVAQRDSLVNSLQPWVFNTIEQLQLIKSNLQALYPLDSLPESAKYLSTKV
                     AEAERDLGNFRDLAKVDVSQMELSLVANLVEKLKSYHQMVEENVKLVCGVRPELCVRA
                     LDVNKSQPADSLKAVDSCSI"
     gene            4442..5827
                     /gene="glmM"
                     /locus_tag="PK502_07575"
     CDS             4442..5827
                     /gene="glmM"
                     /locus_tag="PK502_07575"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPH00714.1"
                     /translation="MALIKSISGIRGTIGGNPGDNLTPLDIVKFTSAYAVWLKQRNPT
                     KRCRVVVGRDARISGEMVNNLVVGSLMGCGLDVVNLGLATTPTVEMAVIHKKTDGGVI
                     LTASHNPRHWNALKLLNEKGEFLSDADGHHVLDLAEGDTLNFAAVDDLGLVEEDSSFI
                     DEHISQILKLRYVDVDAIAGANFTVAIDCVNSVGGLAIPKLLNALGVERVIELYTEPT
                     GNFPHNPEPLPEHLGEISAVVRERKANVGFVVDPDVDRLAIVNEDGSMFGEEYTLVAC
                     ADYVLSHNPGNTVSNLSSTAALREITRRWGKEYYTSAVGEVNVVERMKEVNAVIGGEG
                     NGGVILPELHYGRDALVGIALFLSHLARSGKKCSSLRAAYPSYFISKNKINLKPGVNP
                     DELLAKLLEQLKQYPVNTTDGLRVDFDDSWVHMRKSNTEPIIRIYAEAQNPEKADELA
                     KKMMEQIVKMF"
     gene            complement(5927..7402)
                     /gene="ftsZ"
                     /locus_tag="PK502_07580"
     CDS             complement(5927..7402)
                     /gene="ftsZ"
                     /locus_tag="PK502_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPH00715.1"
                     /translation="MGEELINFDLPATTESIIKVIGVGGGGGNAVNYMHRMGIKDVDF
                     VLCNTDAQALFNSPVKMKLQLGASLTEGRGAGNKPEIGRQAAIESSQQISDLLLRNTK
                     MVFITAGMGGGTGTGAAPVIAQIAKELGILTVAIVTIPFRNEGKKRIAQAIEGIENIR
                     QHVDSLLVVNNEKILEIYGNLKVTEAFSKADDVLATAAKGIAEIITVHGYINVDFADV
                     ETVMANSGVALMGSGRAAGENRAITAVKAALESPLLNNNDISGARNILLNVTYGQDEI
                     TMEEIGLIIEYVQSAAGDDADLIWGNGCDPQLGDDLKVTVIATGFSSSSIPELYIRQK
                     KVDVLNLDGDIFANRVEKTPLVGGSRLQEANPGIPKVKRNEPEDGRQRVIEFDLSSDE
                     IQVKTIGTPKASTPEIPLPVSKTTGKPASQPQKPDSQQGKGKYGDIDELENVPAYKRK
                     NMPLDPVDHSKPDSISRLTLNEEDGDIFLRPDNSFLHDNVD"
     assembly_gap    7479..7578
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7655..8971)
                     /gene="ftsA"
                     /locus_tag="PK502_07585"
     CDS             complement(7655..8971)
                     /gene="ftsA"
                     /locus_tag="PK502_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPH00716.1"
                     /translation="MAHSNDYVVAIDLGTTKVVTMVGKMNSNGKLQVVGLSTAESTGI
                     KRGMIQNIEETVKAIQKTVDDVKKQVGLEFHSAYVGIAGQHIRSIKNSNYLNFDTDDR
                     EISQDDIDRLVKDMNLTPVDAGETILHVLPLEFFVDKEPVDNNRPVGMMGRRLDANFH
                     IVIGKTSSAKHIEKCVNRVGLSVADLILEPLASAEAVLTDDEREAGVALIDIGGGTTD
                     LAIYYEGNIRHTAVIPFGGNVITQDIKEGCSILLKQADQLKEIYGIALSDIAPDNKII
                     TIPGISGREPKEISVKNLAGIIQARMEEILDFVLYEINLSGYAEKLSAGVVITGGGAL
                     LKHLSHLIKFKSGYDVRLGFPSEKLSSDSLEKYNHPQLSTALGLALIGLERQAATPVQ
                     DDAKQQNATEPSVDKEFSEVHEKKGSKFNAIKQMISGLFEETDSEM"
     gene            complement(8976..9782)
                     /locus_tag="PK502_07590"
     CDS             complement(8976..9782)
                     /locus_tag="PK502_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00717.1"
                     /translation="MNRRTRNILIILGWLLLGSYLVYSLLFTAERAKKVLCRGIRVEV
                     VDSAQYQFVKPGTIRKMILSSTISPIGKPLNSINTHKLEKNISELVAVRDVQVYKSMD
                     GILRITVKQRRPIVRIFNARNQSYYIDDEGLVMPVSSSYAAYVLIASGNIYEPFRVKP
                     NIDITRWNDSLTNGSKPLVCQIFDFARYINHDAFWRAQINQIYVINARNVELIPVVGP
                     HVIAMGSLEGYDRKLKKLKLFYENALPTEGWNKYSRINLMYSNQIVCTKR"
     gene            complement(9785..>10858)
                     /locus_tag="PK502_07595"
     CDS             complement(9785..>10858)
                     /locus_tag="PK502_07595"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPH00718.1"
                     /translation="QVLGMLSLGYQTLAVAGTHGKTTTSTLLAYLLGSSSVGCDAFLG
                     GISKNYGSNLVTADKGRKLLVAEADEYDRSFLNLHPQLAVITSVDPDHLDIYGDYERV
                     VEAFNQFAGQVKPNGTLIAKSGLGIAPIPERGVTRLTYGLSSDADFYPVNLSLNEGCY
                     GFSLETPNGTIDDLKLGVPGLFNLENSIAAAAAAITCGISHDELRSGLLSFSGVSRRF
                     DIRFKSSRTVYVDDYAHHPREIEAMIESARQVYPNRKITGIFQPHLYSRTRDFAFDFA
                     RSLDKLDMAILLDIYPAREQPIEGITSQTIADKMNNPNRLLLDKADLLEWLRKNDVDV
                     LLTMGAGDIDRLAPEIVKIFEAK"
BASE COUNT         2786 a         2686 c         2579 g         2707 t
ORIGIN      
        1 tgattcttaa actcctgagc ccgggtggct cttggatgta gcccacccct gttcccgctt
       61 caaccaaatg cccggagcaa ccagcgcagg gtggcagggt tgattttcgg gtttccatcg
      121 tggcctttct aagctcgcac tatgctccct tcagcattcc ggccggctgg tcggaaatgg
      181 gctcaaccct tcagcccagc tggatccgtt cctgtagccc tttgaaccaa aaatctccca
      241 ggctggtatg atttagccaa aaatggctaa ctttcgccat caaattgcca taaagatgca
      301 gcccaagcgt tttgccctgg ttgttgccgc tggctccggc ttacgtatgg gctccgacac
      361 cccaaagcag ttcatggagc tggatgggtt gccggtgctg atgcgcacta ttagcgtttt
      421 cctcaccctg agggatatac ccgaggttgt gctggtgctt cccgaagctc aaatcacaac
      481 atggcaaagg ctctgcaaaa agcaccattt tgaaaccaac ctgcaaattg ttccgggggg
      541 tagcacccgc tttgagtcgg taaaaaacgg tttggagctg ctgcccgcag acgactcgct
      601 ggtggccatt cacgacggcg ttcgtccgct ggttacacat gaggtggttg agcggtgcta
      661 cgccatggct gccagggggg tgtgcgccat tccggttgtg aatcctgttg aatcggtaat
      721 gattgcaagc ggcggcaagt ttaccccatt cccccgcaac aacgttttgc tggtgcaaac
      781 accccagact tttcgctcag caaccataaa gcattgctac aaccagcctt acagcgagtt
      841 gtttaccgac gatgcctcgg ttgctgctgc ctgcggggtt gagctccata ccgtggaggg
      901 aaacagggaa aatatcaaaa taaccacacc ggccgatttg cttaccgccc gggtcctgct
      961 acaagccatg aaaaccgatt aatagccata gctaaccgat tgtgccatga gttttgtatt
     1021 gaaagaggtt gacaaccgaa aaacccgtaa agatttttta aaccttccgg tagagcttta
     1081 ccgcgattat cccagctgga tcaggccgct caacaacgat attgaaagcg ttttcaaccc
     1141 aaaaaccaac aagcagttca ggcatggcga agccattcga tgggtgctat actccgataa
     1201 caagcctgtt ggacggattg ccgcttttat tgactacgag atagcccgca acaacgagca
     1261 gccaactggc ggggttggtt ttttcgagtg catcaacagc cccgaagctg ccacacagct
     1321 attcgacacc gcaaaggagt ggctgcagca aagaggaatg gaggccatgg acggccctat
     1381 caactttggc gatcgcgacc gttggtgggg gctgctggtt gagggcgact acccaccaaa
     1441 ctactgcatg gactaccatc ctccctacta ccgcgagctt tttgagggct acggttttaa
     1501 gccttacttt caccagttca cctactaccg tccggtgaat cccgaagggc ttgacccaat
     1561 tatacgcgaa aaggcggaac gcatagcccg taaccccgcc taccatgtta ccatcatttc
     1621 caaacgcaac cttaaaaagt ttgccgagga tttccgtaca atatacaata acgcctgggg
     1681 aaggtacacc ggggtaaaaa aaatgtcgca cattcatgcc atggccctga tgaaaaccct
     1741 gaaaccaatt atagaccccg agctgatgta ctttgcctac tacaacaatg aacccatcgg
     1801 gttctttatc atggtgcccg atttaaacca ggtggttagg catttgaacg ggcagtttgg
     1861 tctggttcat aagctcaaat ttttatacct gctgaaggtt aagaaggttt gcaccaaggc
     1921 cattggcctg atttttggaa ttgtgtccca acatcagggc aagggtgtag aaggtgctct
     1981 ggtaacagag tttgccaaca gggcactaag acctgacttt cagtacaagg agctggagct
     2041 gaactggatt ggcgacttta accctaccat gcgaagggta gccagccaga taggagggaa
     2101 aatacgtaaa atccatatca cctaccgcta catgttcgac agaaccaagg aggtggtccc
     2161 tccccgaagg gtgagctgag ggtggacaaa agaaacaggc tggttgaacg ctccgatgaa
     2221 atgcgctatg ctaaatattt aattttgaag ctagctatga tgaagcagaa tgctgttata
     2281 aacccacgta tgccgatgtt aaaacgattt ctcgggttgc ttcttttaac attatcgggc
     2341 tatatggttg ctgcccagcg cgatatagaa cctcggctag tattttccga cacctccagg
     2401 ttcaacttta caggcgaatg gcagtacctg tcaaccgata tcttcctgct caacggcgac
     2461 aggtttagca acctaatcaa cgagctcaac tttcctaata agggaaatgg caaaatacgc
     2521 cgaaaatcga agctcgagga ggaacgcctg gagtacctgt tcatcacggc gaagctgaaa
     2581 aatgtgaaat ttttcgggga gaacgacatc acctacccac tctacaactt ccagatatcg
     2641 agagacaagg acaaccgtta ccaaaccctg gtgagcgaca acatcgacca tgtgcggata
     2701 atagacaacc tacccctcta ctccgcacac gattacattg atgcggagat tagcgttaaa
     2761 gccattacca acaacgatcg cgaccagatg ctctcgctcg ttgccaccca gctgcaaaat
     2821 ctatccaaaa taaaaacccc taccgatgcg gtgatgggca ttataggaga attcggcagc
     2881 tttattgagg ctaataccag gaaaaaggag tataggttta gctccaccat caggctattt
     2941 gagcagaaaa attttgatac ccgcgttcat tccttgaagc tatacgtttt aaccacttcc
     3001 aacagcaagc ccattgaagt taacacagct cttatacaaa actttatgga caccgtaagg
     3061 catggccagg ttgggcgcca aacgctgaaa gagctacttg cataccacaa ctacccggta
     3121 atagcggtgg ttaactacaa gtcgctctac cgcatggagc caatcggtgg cgatgaggta
     3181 acctttgcca gcatcgaaac gcggaagctg agggttgaaa acgactatcg gcaggggctg
     3241 ataaacgccg aaacctaccg gcaggagaag gatttcatcg ccttcctgtc cgtttacgcc
     3301 aacttcaaga gttacctcga tgcctataac cttaactacc gaaccggtaa cagcgacgcc
     3361 gtttccataa gccttttcaa ggtgatgcag tactaccggc agctggttaa gacctttgag
     3421 gagatgcgat ttaagtacag ggggaacaac acctttgcaa atatctttag acgagagtat
     3481 gagtctgtgc taggatttgc ctcgctatac ctcgacgatg accacaacct gaaagcaacc
     3541 aaggagctgg tgaactccct ggtaaaactc gacaccatcc caatgcttgc tgctgccgac
     3601 atggaggcat acattgcctc actccgcttc tcgggggcat tcaagcctga ccttatgagg
     3661 caaacacccg agggtcagct gatcaccaac caccttgcca gccttgaaga acaactctac
     3721 aggagcacat acgaggatga ggttagaaag ctgaatgatg ttgaagccag cgttagcacc
     3781 cgtaacgcca ccgagaaact tttggcgatg attaagaata cctcatgcgg gctatgccgt
     3841 gagcgggcca ttgccgcaat caactccttt ggccaacgaa tggatgagtt caccaggaaa
     3901 cagaaggttg cccagcgtga tagcctggta aacagccttc aaccctgggt tttcaacacc
     3961 attgaacagc tacaacttat aaaaagcaac ctccaggcgc tttacccttt agattccctt
     4021 cccgaaagtg caaagtacct cagcacaaaa gttgctgagg ctgaacgcga tttgggtaat
     4081 ttccgtgatt tggctaaagt tgacgtatcg cagatggagc tgtccttagt ggctaacctg
     4141 gttgaaaagc taaaaagcta ccaccagatg gtagaggaaa acgttaagct ggtttgcggg
     4201 gttagaccag aactctgtgt tagggcatta gacgtcaata aatcccaacc agctgattcg
     4261 ctgaaagcgg ttgattcgtg ctccatttaa gccgatgttg tggaagtttg aatgcctgca
     4321 gctggagtag ctaaaaattt aaacctcctg gtgcaaatat tgaggaggaa aaacctaaca
     4381 tttacaacct tgaggcaaat aattgataga aacttataaa aattaactgg agaaactaga
     4441 catggcgctg attaaatcta tatcgggaat tcggggaacc attgggggaa atccgggcga
     4501 caacctaaca cccttggata ttgtaaagtt tacgtcggca tacgccgtat ggttaaagca
     4561 acgcaatcca accaagcgct gcagggttgt agttggcagg gatgcacgaa tttcgggcga
     4621 aatggtcaac aacctggttg ttggttcgct aatggggtgc ggtttggatg ttgttaacct
     4681 tggcctggcc actactccta cggttgaaat ggctgtaatc cataagaaaa ccgatggcgg
     4741 agtaattcta acagccagcc ataacccccg ccattggaac gccctaaagc tgctgaacga
     4801 aaaaggtgaa tttctctccg atgccgacgg gcatcatgtg cttgaccttg ccgaaggcga
     4861 tacccttaac tttgcagcag ttgacgatct ggggctggtt gaggaggata gctctttcat
     4921 tgatgagcat atcagccaaa tactcaagct gcgttatgta gatgttgacg ccattgccgg
     4981 tgctaatttt acagtggcta tagactgtgt aaattcggtt ggaggactag ccattccaaa
     5041 gctgttgaac gcattgggtg ttgaaagggt gattgaactc tacaccgagc caaccggtaa
     5101 ttttccacat aaccccgaac ctcttccaga gcatctgggc gaaatttcag ctgttgtacg
     5161 tgagcgcaag gctaatgtag gctttgtggt tgaccccgat gttgaccggc tagccattgt
     5221 taacgaggac ggaagcatgt ttggtgagga gtacaccctg gtggcctgtg ccgattacgt
     5281 gctcagccat aacccgggta ataccgtttc caacctgtcg agcaccgcag cgcttcgtga
     5341 aataacccgt agatggggaa aggaatacta cacttcggca gtgggcgagg taaatgtggt
     5401 ggagcgaatg aaagaggtga atgccgtaat tggcggggag ggtaatggtg gagttattct
     5461 accggagctg cattatggaa gggatgccct ggtgggtatt gcccttttcc tgtcgcatct
     5521 tgcccgatcg ggcaaaaaat gctcctccct aagagcggcc tatcccagct acttcatctc
     5581 aaaaaataaa attaacctga agccaggcgt caaccccgac gagttgctgg caaagctgct
     5641 ggaacagcta aaacaatatc ccgtaaacac aaccgatggg ctgagggttg acttcgacga
     5701 ctcatgggtt cacatgcgca agtcgaacac cgaacccatc atcaggattt acgccgaagc
     5761 ccaaaacccg gaaaaagccg acgagctggc taaaaaaatg atggagcaaa tcgtaaagat
     5821 gttttaacca tgcaaccatc agtaaccagg tagcagggct ttggttggca aggcagctaa
     5881 ccggcaacag cgctgagcca acccgattca acattggcgc taatttctag tcaacattat
     5941 cgtgcaggaa cgagttgtcg ggccgaagga atatatcgcc atcctcctcg ttaagggtta
     6001 acctggatat gctatcgggt ttagagtggt caaccgggtc gagtggcatg ttttttcgct
     6061 tgtatgcagg cacattctcc agctcatcaa tatcaccgta tttccctttg ccctgctgtg
     6121 aatcgggttt ttgaggctgg ctggcgggtt ttccggttgt tttagacacc ggaagcggaa
     6181 tttcaggagt ggaggcctta ggcgtgccta tcgttttaac ctgaatctca tccgatgaga
     6241 ggtcaaactc aataacccgc tgacggccat cctccggctc gtttcttttt accttaggaa
     6301 taccaggatt agcctcctgc aaccggctgc cgccaaccaa aggagttttt tcaacccggt
     6361 tggcaaatat atcgccgtca aggttaagca catcaacttt tttctggcgt atgtagagtt
     6421 ccggaatgct gctggagctg aaaccggtag caattacggt aacctttagg tcatcgccca
     6481 gctgtgggtc gcagccatta ccccatatca aatcggcatc gtctccagcg gccgattgaa
     6541 cgtactcaat aataagccca atctcctcca tggtgatttc atcctgtccg taggtaacat
     6601 tcagcaatat attacgggca cctgaaatgt cgttgttgtt aagtagtggc gattccaacg
     6661 cagcttttac cgctgtgata gccctgtttt caccggcggc ccttcccgaa cccataagcg
     6721 ctaccccgct gttggccata acggtttcaa cgtcggcaaa atcaacgttg atgtagccgt
     6781 gaaccgtaat tatctcagct attcccttgg cagctgttgc cagcacatcg tcggcttttg
     6841 agaaagcctc ggtaacctta agattcccgt aaatttcaag aattttttcg ttgtttacca
     6901 ccagcagtga gtccacgtgc tgacgtatgt tttctatacc ctcaatagct tgagctatgc
     6961 gctttttccc ctcatttcta aaaggaattg taacaatagc cactgttaga atacccagct
     7021 ccttcgctat ctgtgcaatt acgggtgcag cacctgtccc ggttccgcca cccatccctg
     7081 cagtgataaa aaccattttg gtgttgcgta aaagcaaatc ggaaatttgc tgggagctct
     7141 caatggcggc ctgcctgcca atttcgggtt tgttgccggc tccgcgtccc tcggttaaag
     7201 aggcgcccag ctgaagcttc atcttaacgg ggctattgaa aagtgcctgg gcatcggtgt
     7261 tgcatagcac gaaatcaaca tcctttatcc ccatgcggtg catgtagttt acagcatttc
     7321 caccgccccc tcccacgcca ataaccttaa ttatggattc ggttgttgca ggtaaatcga
     7381 agttaatcag ctcttcgccc atggcttaac tatttagtaa tatttataat tcaaaattta
     7441 gtccggcttt tgacggaact tgtccgccta ctggcggann nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnncg gaacttgtcc gcctactggc ggattctccc ttctacagtc
     7621 tcgggattca gcatttcggt tcctggtgca cggttcacat ttcgctatcg gtttcctcaa
     7681 aaagtcctga aatcatctgc ttaattgcgt tgaacttgct gccctttttt tcatgcacct
     7741 ctgagaactc cttgtcaaca gatggttcgg tggcattttg ttgtttggca tcatcctgca
     7801 caggagtggc agcctgtctt tcgagtccta ttagcgccaa acccaatgcg gtggatagct
     7861 ggggatggtt gtatttttca agggagtcgg acgaaagttt ctcactaggg aaccccaacc
     7921 ggacatcgta acccgattta aacttaatga ggtgcgagag gtgtttcagc agcgctccgc
     7981 ctccggtaat caccacgccg gcgctaagct tctctgcata gcccgaaagg ttaatttcgt
     8041 agaggacaaa atccagaatt tcctccatgc gggcctgaat gatgcctgcc aggtttttaa
     8101 ccgaaatttc cttgggctcc cgcccgctaa taccaggaat ggtaatgatt ttgttatcag
     8161 gtgcaatgtc ggagagggct ataccgtaga tttcctttag ctggtcggcc tgtttcagaa
     8221 gaatggagca accctcctta atgtcctggg tgataacgtt tccaccaaaa ggaataacag
     8281 cagtatgcct tatgttcccc tcgtagtata ttgccaggtc ggtagtgcca ccgccaatat
     8341 caataagggc aacgccagcc tcacgctcgt cgtcggttaa aacggcctcg gcagaggcaa
     8401 gcggctcaag aataagatcg gccacactca agcccacccg gtttacacac ttctcaatgt
     8461 gcttggccga tgaagtttta ccgattacaa tgtggaagtt ggcatctaac cttcggccca
     8521 tcatcccaac aggccggtta ttgtcaacgg gctccttgtc aacaaaaaac tccaggggta
     8581 aaacatgcag tatggtttca ccggcatcaa caggagtgag attcatatcc tttacaagcc
     8641 tgtcaatatc gtcctgggag atttccctgt cgtcggtatc aaagttcagg tagttgctgt
     8701 tcttgatact tcggatgtgc tgaccggcaa tcccaacata ggctgaatgg aattcgagtc
     8761 caacttgctt tttaacgtcg tcaactgttt tctggattgc cttaacggtt tcctcgatat
     8821 tttgaatcat ccctcgttta atgccagttg attcggcggt gctaaggccc accacctgca
     8881 gcttcccgtt ggaattcatc ttacctacca tggttaccac ctttgtggtt cccaggtcaa
     8941 ttgccactac atagtcgttt gaatgtgcca tatcactaac gttttgtgca aactatttga
     9001 ttagagtaca taaggtttat tctgctatac ttgttccaac cctcggttgg cagtgcgttc
     9061 tcgtagaaaa gcttcaactt tttcaactta cggtcgtagc cctccaggct ccccatagca
     9121 ataacatgag gaccaacaac gggaatcagc tcaacgtttc gggcattgat aacgtagatc
     9181 tggttgattt gagccctcca aaaggcatcg tggttgatat acctggcaaa gtcaaagatt
     9241 tggcatacca acggttttga accattggta aggctatcgt tccacctggt aatgtcaatg
     9301 tttggtttaa ccctgaaagg ttcatatata tttccgctcg caataagaac ataggctgca
     9361 tagctactcg aaacgggcat taccaagccc tcgtcatcaa tatagtagct ttgatttcgt
     9421 gcgttgaata tgcgaacaat gggtctgcgc tgtttcaccg taattctgag gatgccatcc
     9481 atggatttat atacctgcac atcacgaacg gcaaccagct cggaaatgtt tttttcaagc
     9541 ttatgggtgt taatcgaatt caacggttta ccgatggggg aaatagtgga cgaaaggatc
     9601 atttttctta ttgtaccggg tttaacaaac tggtactggg cgctatcaac aacttcaacc
     9661 ctgatgcccc tgcaaagaac cttttttgca cgctcggcag taaataaaag gctataaacg
     9721 aggtatgagc ctaacagaag ccatcccaag ataataagta tgttgcgggt tcgacggttc
     9781 atatctattt agcctcaaat atttttacaa tttcgggcgc caaccggtca atatcgccag
     9841 cacccattgt caaaagcaca tccacatcat ttttcctaag ccactcgagc aggtcggctt
     9901 tgtccagaag cagcctgttt ggattgttca ttttatcagc aatggtctgc gaggttattc
     9961 cttcaatagg ttgctccctg gcggggtata tatcgagcaa aatagccatg tcgagcttat
    10021 cgagcgaccg ggcaaaatcg aaagcaaaat cccgggtacg gctgtagagg tggggctgaa
    10081 aaatcccggt tattttacgg tttgggtaaa cctgcctggc cgattctatc atggcctcaa
    10141 tttcgcgggg gtgatgggcg taatcgtcaa catatacggt tctactgctt ttaaaccgaa
    10201 tatcgaaccg tcgtgatacg ccagaaaatg agagcagacc gcttcgtagc tcatcatggc
    10261 taataccaca ggtaattgct gctgccgcag cggcaataga attttcgagg ttgaacaatc
    10321 ccggaactcc aagcttgagg tcgtctatcg ttccattagg cgtttcgagc gaaaagccgt
    10381 aacaaccctc gttaaggcta aggttgaccg ggtagaaatc ggcatctgac gacagcccat
    10441 aggttaaccg ggtaacgccc ctttcaggga taggcgcaat tcccagaccg gatttagcta
    10501 tcaacgttcc gttaggttta acctgaccgg caaactggtt gaacgcctcc actacccttt
    10561 cataatcacc gtaaatgtcg agatgatcgg ggtcaaccga tgtaataacg gccagctggg
    10621 ggtgcaggtt caaaaaactg cgatcgtact cgtcggcctc ggccacaagc agctttctac
    10681 ccttatcggc ggtaaccagg ttgctgccgt aattttttga gattccaccc aaaaaagcat
    10741 cgcaaccaac cgatgagctt cccagcaggt aggcaagcag ggttgaggtg gtagttttgc
    10801 cgtgggttcc ggcaacggcc aatgtttgat accctaatga cagcattcca agcacctg
//
LOCUS       DAOPHX010000092        10402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_7852_length_10402_cov_5.200831, whole genome shotgun sequence.
ACCESSION   DAOPHX010000092 DAOPHX010000000
VERSION     DAOPHX010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 10402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10402
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7852_length_10402_cov_5.200831"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PK502_07600"
     CDS             complement(<1..269)
                     /locus_tag="PK502_07600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HPH00719.1"
                     /translation="MSKLPKAPLVEVVFELRWQIINKVDLSKIPYLYGDIYSQLKNKY
                     PFRESIVPPEIPIDILINQPVHRFRTAPNDYPLFQVGTGIITLNT"
     gene            complement(416..1393)
                     /locus_tag="PK502_07605"
     CDS             complement(416..1393)
                     /locus_tag="PK502_07605"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HPH00720.1"
                     /translation="MANLTTSYLGLNLKSPLVVSSSNLTASVERIKNFEEMGAGAVVL
                     KSLFEEQIQHEIGNLAQYSDYTEAADYLKHYVTANNVNTYIELIRKAKSAVGIPVIAS
                     INCMSASQWVTFASQIQEAGADALEVNVFMLPVDPHKTSAEYESIYTSLAEKLTRIIS
                     IPIAFKVGPHFTNPFAVIEKLWYLKIRGVVLFNRFFEPDFDINTLKVIPASIFSHPDD
                     IRNSLRWVGMTNHLVPKIDISASTGVHDGEAMIKMILAGASSVQVCSAIYMNGAKHIA
                     SMLSKLSEWMDTMGFASINDFKGKLNYGQIHDPALYERSQFMKYYSSFE"
     gene            complement(1431..2102)
                     /locus_tag="PK502_07610"
     CDS             complement(1431..2102)
                     /locus_tag="PK502_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPH00721.1"
                     /translation="MSIGTNLLETINSLPKGVTLVAVSKTHPPESIMEAYNAGQRVFG
                     ENKVQELVSKYEVLPRDIEWHLIGHLQSNKVKYVVPFVRLIHSVDSLRLLVTIDREAQ
                     KVNRVVDCLLQIHIASEETKFGLSADELEQLLDAEEYNLLQHIRVVGLMGMATFTENK
                     EQVRMEFRFLRQLFERIQEKYFVGKPWFCELSMGMSSDYRIAVEEGSTMVRIGSSIFG
                     QRVYR"
     gene            complement(2577..>3538)
                     /locus_tag="PK502_07615"
     CDS             complement(2577..>3538)
                     /locus_tag="PK502_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPH00722.1"
                     /translation="KEGRTQYGLVACCHYEDYMEGRIKKHELTRPDKEEDRMIHIRLQ
                     NANIEPVFFAYPAQQEMDEIVNGVVRSCKPEYDFVAADGFGHKFWVVDNDETIRRITG
                     IFDRIPALYVADGHHRTAAAARVALEKKNANPNHRGDEEYNYFMAVIFPDNQLKIIDY
                     NRVVKDLNGLTSEEFIQKIRKGFDIVEVGTQTYKPQKLHNFSMYLDGKWYSLTAKPGT
                     YNDNDPIGVLDVTILSNLILDQVLDIKDLRTSKRIDFVGGIRGLDELKRRVDSGEMKV
                     AFALYPVSMKQLITIADTGNIMPPKTTWFEPKLRSGLVIHSLD"
     assembly_gap    3539..3548
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3549..3835)
                     /locus_tag="PK502_07620"
     CDS             complement(<3549..3835)
                     /locus_tag="PK502_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPH00723.1"
                     /translation="MVKIKSFKGIRPPKELVKEIAARPYDVLNSAEAQKEAGEKSLLH
                     ITKPEIDFNPIIDEHSKEAYDKAVENFRKWKEKGWLVQDKKERYYIYAQTM"
     gene            complement(4273..5196)
                     /locus_tag="PK502_07625"
     CDS             complement(4273..5196)
                     /locus_tag="PK502_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HPH00724.1"
                     /translation="MTKVLVATEKPFAKTAVEGIRKIVEDAGFELALLEKYTDPNDLV
                     KAVTDADALIVRSDKVTKEVVDAAKKLKIVVRAGAGYDNLDLAACSAKGIVAMNTPGQ
                     NSNAVAELAIGLMIYMARNLFQPGTGTEISGKKLGIHAYGNVGKLVAKYGKGFGMAVC
                     AFDPFVSKDEMEKDGVKAVDSIDTLYSKCDYVSLHIPANDKTKKSIGYELLSKMPKGA
                     TLINTARKEVIDEEGLKKALAERPDLKYATDIMPDCHAELVEKFPGRVFATPKKMGAE
                     TAEANVNAGLAAARQIVAFIKNGDKTFQVNK"
     gene            5195..5350
                     /locus_tag="PK502_07630"
     CDS             5195..5350
                     /locus_tag="PK502_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00725.1"
                     /translation="MIVKFLVNVVLEKFVILNKEKYPFPYQNKICTNRNTKSFGKIAQ
                     NDKKAIR"
     gene            complement(5360..6430)
                     /gene="serC"
                     /locus_tag="PK502_07635"
     CDS             complement(5360..6430)
                     /gene="serC"
                     /locus_tag="PK502_07635"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HPH00726.1"
                     /translation="MKKHNFNAGPCILPEVALQNTAKAVIDLDGIGMSVLTISHRSKE
                     FEAIMDETKALWRELLHIPDNYHILFLGGGASLQFCMVPYNLLQKKAVYLETGVWAKK
                     ALQEAKIFGDATALASSADKNFTYIPKGFKIPTDVDYFHVTTNNTIYGTELKEDIDSP
                     VTLVADMSSDIMSRPVDIKKYGLIYGGAQKNVGPAGVTFVIIREDILGKVDRKIPTML
                     DYRTHIKEDSMFNTPPCGAIFTMRETLKWVKSIGGVEKINELNKKKAQLLYDAIDSSK
                     MFVGTVEKDSRSIMNICFVMKPEYKDKEDAFMDFAKKAGMVGIKGHRSVGGFRASTYN
                     ALPIESVQALVDCMREFEKQNA"
     gene            complement(6561..7274)
                     /locus_tag="PK502_07640"
     CDS             complement(6561..7274)
                     /locus_tag="PK502_07640"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HPH00727.1"
                     /translation="MNTIEVALSQSEFGCFDPKSKIVVVVDILRATSVICTMFKNGVS
                     EIIPVRSIDEAKTYKQKGFMVVAERNGQKLDFADFGNSPFYFTPDKVSGKTVVYSTTN
                     GTNAIMEGSKADTVIIASFLNFSSIFEFLKGGQSDVLILCAGWKGKFCLEDTIFAGAL
                     AYSLINSGNFTTICDSTNAAIDLWKVASPDMHTYLEKVAQRHRLKKLGLDDVIDYCFT
                     MDQTTAIPVLKGGSLIRLC"
     gene            complement(7324..8433)
                     /gene="gcvT"
                     /locus_tag="PK502_07645"
     CDS             complement(7324..8433)
                     /gene="gcvT"
                     /locus_tag="PK502_07645"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPH00728.1"
                     /translation="MKNTPFTKYHIELGARMAPFAGYNMPIEYSGISDEHITVREKVG
                     VFDVSHMGEVWVKGPNALEFLQTVTSNDVSALTNGKVQYSCFPNDKGGIVDDLLVYRF
                     DEQTYFLVINAANIEKDWSWLCQQGQRFGLTPGKELYNASDEFAQLAIQGPLALKAMQ
                     KLTSANVTDMEYYTFKKISFAGIREVIFSTTGYTGAGGCEIYCSPEDADKLWKAVFDA
                     GKEFGIKPIGLGARDTLRLEAGFCLYGNDLNDSTSAIEAGLGWITKFVPGKNFISREL
                     WEKQKNEGTERRLKGFVMIDRGVPRQHYPIFDAQGNPIGEVTSGTMSPLTKLGIGMGY
                     IKKGFWNDGSEIFIGIRDKKLKAKIVKPPFHQVKL"
     gene            8775..9143
                     /locus_tag="PK502_07650"
     CDS             8775..9143
                     /locus_tag="PK502_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HPH00729.1"
                     /translation="MGMTSNEAAKHVNLEILKEISDGNTDLMVDLINLFIGQVRTFSD
                     QLELYNRKKDYECMAKLAHKIKNSVAMMGIDELSSDMKKLERLAQEGKKTNLYDNFIE
                     RFKKISGEAVIELKAILNSL"
     gene            9162..9497
                     /locus_tag="PK502_07655"
     CDS             9162..9497
                     /locus_tag="PK502_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPH00730.1"
                     /translation="MIEVEKINDAMVISFGSDNKLNVTVTQKIKTEIIKLIDPNSKII
                     LNLSGVSYIDSTGFGMLLSILRHCKNSNSQLKLCNISPEVMELIKLLQLQNIFDIREG
                     VNESLKSFC"
     gene            complement(9641..>10402)
                     /locus_tag="PK502_07660"
     CDS             complement(9641..>10402)
                     /locus_tag="PK502_07660"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HPH00731.1"
                     /translation="WILNPFLNTADKYNWSMGNPYLEPEFTTSLDFNYGLNFPSTKVS
                     ASVYYRDTQNGFSRKLALVDSVTTLSTFINLSHNKSYGVEGVVTQNLTKWWRLNASYS
                     YFKTKLYGDIVNGADEGTAWTAKLTSFFNIKKKIDIQVNGNYRSPILSASGTGNGFHS
                     IGGAQGKTKEMYWVDLGARISVLNNKGTITVRASDIFNTQKSRSNSWDTNFTSYSENW
                     RNSQMLFVGFSYKINNYKARKVNDAEMDQTNDFME"
BASE COUNT         2793 a         2385 c         2182 g         3032 t
ORIGIN      
        1 atggtgttta aagtaattat acctgtgcca acttgaaata atggataatc atttggagct
       61 gttctgaatc tatgaacagg ttgattaatt agaatgtcaa ttggaatttc tggaggaaca
      121 atactttctc taaacggata tttattcttt agttgagagt aaatatcacc atatagatat
      181 ggaatcttag ataggtcaac cttatttatg atttgccatc taagttcaaa aacaacctca
      241 actaacggtg ctttaggcag cttgctcata tttctccctg tttaatacac caaatataca
      301 atattcgtat caaaaatgat acactgaatc gactgtttta aggtttttta agtattgtcc
      361 ccaacactct aacaaacttt cttacctaat acaacattcc gttttaaggg aaggcctact
      421 cgaagctgga gtagtacttc atgaactggc tacgctcgta gagtgcagga tcatgaattt
      481 gaccgtagtt caatttccct ttaaaatcgt tgatgctggc aaatcccata gtatccatcc
      541 attcggacag cttactaagc attgaggcaa tatgctttgc tccattcatg tagatagccg
      601 agcaaacctg aaccgacgag gcgccagcca gaatcatttt tatcattgcc tcaccgtcgt
      661 gaactcccgt agaagccgag atgtcaatct ttggcaccag gtggtttgtc attcccaccc
      721 accgcagcga gttccgaata tcgtcgggat ggctgaaaat agatgcgggt ataaccttta
      781 gggtgtttat gtcgaaatcg ggttcgaaaa accggttaaa aagcaccact cccctaatct
      841 tgaggtacca aagcttttca atcaccgcaa aagggttggt aaaatgtggt cccaccttaa
      901 aagctatggg tatggaaatt atccgggtaa gcttctcggc caggcttgtg tagattgact
      961 cgtattcagc agaggtttta tgggggtcaa ctggtagcat gaacacgttt acctccagcg
     1021 catcggcacc cgcctcctgt atctgcgagg caaaagtaac ccactgcgaa gcgctcatgc
     1081 agttgatgct ggcaataacc ggaataccga cagctgattt tgccttgcgt ataagctcaa
     1141 tgtaggtgtt cacgttgttg gcagtaacgt aatgcttcag gtagtcggct gcttcggtat
     1201 agtcggagta ctgcgccagg ttgccaatct cgtgttgaat ttgctcttcg aacagcgact
     1261 tcagcacaac ggcgcctgca cccatctcct caaaattttt aatccgctca accgaggcgg
     1321 taaggttgga gctgctcacc accagcggcg attttaggtt taaccctagg tatgaggttg
     1381 taaggtttgc catagctgta ttttttagtt gatttatggt tacgatgaac ttacctgtaa
     1441 actcgctggc caaaaatgct gctgccaatt ctcaccatgg tgcttccctc ctcaacagca
     1501 atcctgtagt cggaggacat gcccatggag agttcacaaa accaaggttt cccgacaaag
     1561 tatttttcct gtattctttc gaaaagttgc ctgaggaacc tgaattccat tctcacctgt
     1621 tccttgtttt cagtaaaagt agccattccc atcaatccaa caacccgaat atgctgcagc
     1681 agattatact cctcagcatc caggagctgt tccagctcat cggccgacag cccaaactta
     1741 gtttcctccg aggctatgtg gatttgcaac aaacaatcca caactcggtt aaccttttga
     1801 gcttctctat caatggtaac cagtagcctt aaactatcca ccgagtggat tagccgtaca
     1861 aagggaacca cgtactttac cttattcgat tgaagatggc ctattagatg ccactcaata
     1921 tctcttggca acacttcgta cttcgatacc agctcctgca ccttattctc cccgaaaacc
     1981 ctttggcctg cattatatgc ctccatgatg ctttcgggag gatgggtttt tgaaacggca
     2041 accagcgtaa ccccttttgg caacgagttt atagtttcaa gaagatttgt cccaatactc
     2101 attacagtaa ggtttgatgc aaatatagaa tattttgcct atgggcaaaa ggaaaaattt
     2161 tgccgctcat cgctcttaag tcctaacctt aactccttgc aagtcgattt tttttctcaa
     2221 actctgtcaa ttctctgata aagtgatgtt tatcgttatg ataccaacca gcgtttttcc
     2281 tgttaattca acctttttct attgattgct ggcatatcat aagattctcc ttgccaccga
     2341 taacatcctg caagtaatat tccgactaaa ttgcaaaccg ctgtgtagct tggccccgct
     2401 ttgttgctac ttgagcatcc caacagctac cctagcataa caggttgtag tgattttttt
     2461 ttagctgatt caacagcgta gtaaaattta tgtgtttatt tacgcttaag taagaatttc
     2521 tcttagtgta gtgccatttt atagcaaata aatgggctga cttgcagccc atttatctaa
     2581 tctaaggagt gaataaccaa cccgctgcga agcttaggtt cgaaccatgt agttttgggt
     2641 ggcatgatgt tgccggtgtc ggcaatggta atcagctgct tcatcgaaac ggggtaaagg
     2701 gcaaaggcca ccttcatttc gccactgtca acacgccttt ttagctcgtc cagcccgcgg
     2761 atgccgccaa caaagtctat ccgtttcgat gttcgcaggt ccttgatgtc gagcacctga
     2821 tcgaggatga ggttggagag aatggtaaca tccagcacgc caatggggtc gttgtcgttg
     2881 tatgtacccg gtttggcagt tagggaatac cacttaccat caaggtacat gctgaagttg
     2941 tgcagcttct gcggtttata ggtttgggtg ccaacctcaa caatatcaaa ccctttcctg
     3001 atcttttgaa taaactcctc ggaggttagg ccattcaaat ccttaacaac acggttgtaa
     3061 tcaattattt tcagctggtt atccggaaag attaccgcca taaaatagtt gtactcctca
     3121 tcgccccgat ggtttgggtt ggcattcttt ttttcgagag caacacgggc ggctgcagcc
     3181 gttcgatgat ggccgtcagc cacgtaaaga gcaggaattc ggtcaaatat accggttatg
     3241 cgccgaattg tttcatcatt atcaaccacc cagaacttgt gtccaaatcc atcagcggct
     3301 acaaaatcgt actcgggttt gcagcttcta accacgccat tgacaatctc gtccatttcc
     3361 tgctgagcgg ggtaggcaaa gaataccggt tcaatattgg cattctgcag cctgatatgg
     3421 atcatcctgt cctcctcctt atcgggacgg gttagctcgt gcttcttaat cctaccctcc
     3481 atgtagtcct cataatggca gcatgcaacc aatccgtact gcgtacgacc ctccttggnn
     3541 nnnnnnnntc catggtttga gcatagatgt agtatctctc cttcttatct tgaaccagcc
     3601 agcccttttc cttccacttc ctgaaattct caaccgcttt gtcgtaggct tccttggaat
     3661 gctcatcaat aattgggttg aagtctattt cgggcttggt aatgtgaagc agcgatttct
     3721 caccggcttc cttctgagct tcagccgagt taagcacatc atacgggcga gcggcaattt
     3781 ccttaaccag ctccttaggg ggccgaatgc ccttgaatga ttttattttt accatgaaaa
     3841 gttaattgtt aagagttgaa aagttaaaag acaataatgt taaacggttg attttaaggc
     3901 ttttaatcac gccaaaattt aattgcttaa ctaaaagaat atgcttcata atttctgata
     3961 tattcgatat ataccctagc ctattctaag ttattagctg tgtttctaat tcggctaaag
     4021 attcagctgc aaaataaaga aaatgaattt gtttctgccg aactatttct tgcacagccc
     4081 acagcaatat ttgatggaat agaaactact gctcgactaa tctgagaggt taacccctaa
     4141 gtctcttctt taggaaaaga tttagttagt tcataaattt caactgttaa ctctacagcc
     4201 tctttccaag cctctagctt agtatgctcc accttctcgt ttaacttttc acttttaact
     4261 tttcacctgt tattacttat tcacctgaaa agttttatcg ccattcttga taaaggcaac
     4321 tatttgacgg gcggcagcca ggccagcgtt aacgttggct tcagcagttt cggcacccat
     4381 tttcttgggt gttgcaaaca cccttccggg gaatttttcc accagctcgg catggcaatc
     4441 gggcataatg tcggtggcat atttcaaatc gggacgttcg gccaaggctt ttttcaaccc
     4501 ctcctcatca ataacctcct tgcgggcagt gttgattagt gtagcgccct tgggcatctt
     4561 ggagagtaac tcgtacccga tagacttctt tgttttgtcg tttgcaggta tgtgcagcga
     4621 aacgtagtcg cacttcgagt aaagtgtatc aattgagtca acagccttta ccccatcctt
     4681 ctccatttca tccttggata caaacgggtc gaaggcgcaa acagccattc caaatccctt
     4741 gccgtactta gccaccagct tacccacgtt gccgtaggca tgaataccaa gttttttgcc
     4801 tgaaatctcg gtgcctgttc ctggttggaa aaggttacga gccatgtata tcatcaggcc
     4861 aatggcaagc tcggcaactg cgttggagtt ttgcccaggt gtattcatgg ctacaattcc
     4921 cttggctgag caagcagcca aatcgagatt gtcgtagccc gcacctgccc gtaccacaat
     4981 cttgagcttt ttagcggcat caaccacctc tttggtaacc ttatcgctac gaactataag
     5041 ggcatcggca tcggtaacgg ccttaaccag atcgtttgga tcggtatatt tttcaagaag
     5101 agccaactca aaaccagcat cctcaacaat ttttctaatt ccttcaaccg cagtctttgc
     5161 aaaaggcttt tcggttgcaa ctagtacttt tgtcatgata gtaaagtttt tggttaacgt
     5221 tgtgttagaa aaatttgtca ttctgaacaa ggagaagtac cccttcccct accaaaacaa
     5281 gatttgcacc aataggaata caaagagttt cggcaaaatt gctcagaatg acaaaaaggc
     5341 catacgataa aggagtaagc taagcgtttt gcttttcaaa ttccctcata cagtcaacga
     5401 gagcctgaac gctctcaatt gggagtgcgt tataagttga tgccctaaat ccgccaaccg
     5461 aacggtgtcc cttaattcca accataccgg cttttttggc aaaatccata aaagcatctt
     5521 ccttatcctt gtactcgggt ttcatcacaa agcatatgtt catgatagag cgtgaatcct
     5581 tttcaacggt gcccacaaac atcttgctgc tgtcaatggc atcgtagagc agctgggctt
     5641 ttttcttgtt tagctcatta attttctcaa cgccgccaat gctcttgacc cacttcaacg
     5701 tttcgcgcat ggtgaagata gccccacagg gaggggtgtt gaacatggaa tcctccttga
     5761 tgtgggttcg gtagtcgagc atggttggaa tctttcggtc aaccttaccg agaatatcct
     5821 cccgaataat aacgaacgta actccggcag gccctacgtt cttctgggct ccaccgtaga
     5881 ttaacccgta tttctttata tcaacagggc ggctcataat gtcggagctc atatcggcaa
     5941 ccagggtaac aggtgaatcg atatcctcct ttagctcagt tccataaatg gtgttgttgg
     6001 tggttacgtg gaagtagtca acatcggtag ggattttaaa gcccttgggg atgtaggtaa
     6061 aattcttatc ggcagaagag gctagtgcag tggcatcgcc aaaaattttt gcctcctgca
     6121 gggctttctt tgcccacact ccggtttcga ggtaaacagc ctttttttga agcaggttgt
     6181 aaggaaccat gcagaactgc aggctggcac ccccaccaag gaataggata tggtagttgt
     6241 cggggatatg aagcaactcc cgccacaagg ctttagtttc atccataata gcttcaaatt
     6301 cctttgaacg atgggaaatt gtcagaaccg acatccctat tccatcaaga tcaatcacag
     6361 ctttagctgt gttttgaagt gcaacttcgg gcaggataca aggcccggca ttaaaattat
     6421 gtttcttcat ggcatttagg attaaatttt aacagatttc ctcaaagata cgtgaaattt
     6481 taaagttaaa cccaaacatt tcaatgtaaa tgttataaaa catttaccag tgaattccat
     6541 ttgcagggca ggtaggctaa tcagcaaagc ctaataagac tgccaccttt tagcactgga
     6601 attgctgttg tttggtccat agtaaagcag tagtctataa catcgtccaa acccagcttc
     6661 ttaagccgat gcctttgggc taccttttcg aggtaggtat gcatatcggg gctggctacc
     6721 ttccaaagat caatagcggc gttggtggag tcgcaaatgg tggtgaagtt tcctgaattg
     6781 atcagggagt aagctagggc accggcaaaa atagtatcct caaggcagaa ttttcccttc
     6841 caaccggcgc aaagtataag aacatcagac tgcccccctt ttaaaaattc aaagatggag
     6901 gagaagttta agaacgaggc aataataacc gtgtcagcct tactaccctc catgatggca
     6961 ttggtgccgt tggtggtgct gtaaaccact gttttcccgg acactttatc gggagtaaag
     7021 tagaaaggcg aattgccgaa atcggcaaaa tcgagcttct ggccatttcg ctcggcaacc
     7081 accataaagc ctttctgctt gtaggtcttg gcctcgtcaa tgcttcttac cgggataatt
     7141 tcactgacgc catttttaaa catggtgcag attacagagg tagccctaag gatatcaacc
     7201 accacaacaa tcttactctt cgggtcgaag catccgaatt cagattggga aagggcaact
     7261 tcaatggtgt tcactttaca cttggtttaa aagttaaggg ggcaaggccc cctttttggt
     7321 aggttacaat tttacctggt gaaatggcgg cttaacgatt tttgccttca gcttcttgtc
     7381 gcgaattcca atgaagattt cgctgccatc gttccaaaat ccctttttga tgtagcccat
     7441 gccaataccc agcttggtta ggggcgacat ggttcctgag gtaacctcgc caatcggatt
     7501 accctgagca tcaaaaatag ggtagtgctg gcggggtaca cccctgtcaa tcattacaaa
     7561 tcctttaagc ctgcgttcgg taccctcatt tttctgtttt tcccaaagtt cacggctaat
     7621 gaagttctta cctggaacaa acttggtaat ccagcccaat ccggcttcaa tagccgacgt
     7681 ggaatcgtta aggtcgtttc cgtagaggca aaatccagcc tccagcctaa gggtatcgcg
     7741 cgcaccaagc ccaatgggct taataccgaa ctctttccct gcatcaaaaa ccgctttcca
     7801 gagcttgtcg gcatcttcag ggctacagta tatttcgcaa cccccggcac cggtatagcc
     7861 agtggtggag aatattacct cccggatacc ggcaaaggaa atttttttaa aggtgtagta
     7921 ctccatgtcg gtaacatttg cggaagttag cttctgcatg gccttaaggg caagcggtcc
     7981 ctgaatggcc agctgggcaa actcatcgga ggcgttgtag agctccttac ctggagtcag
     8041 gccaaacctt tgcccctgct ggcatagcca actccaatct ttttctatgt ttgcagcgtt
     8101 aataaccaaa aagtacgttt gctcatcaaa cctgtaaacc agaaggtcgt caacaattcc
     8161 ccccttatca ttggggaagc agctatactg cacctttccg ttggttaacg ctgaaacatc
     8221 attgctggta acggtttgta gaaattcaag ggcatttggc ccctttaccc aaacctcacc
     8281 catgtgggaa acgtcaaata cgcccacctt ttcccgaaca gttatatgtt cgtcgctgat
     8341 tccggaatat tcgataggca tgttgtagcc tgcaaaaggc gccatacgtg ctcccaactc
     8401 tatgtgatac ttggtgaatg gagtgttttt catctgctta ctgtttaact ggtagtttta
     8461 tgatatagta acgtattgct tgcatttgaa cataaaccgt tattttaata gaaaacatcg
     8521 gccacctgct ggatgtgagc cggagtggca ttttattggt tggctaacac ccgtatttat
     8581 catgtacact actacacctg caaagctact ttttttaaat gtcttattaa aattaaaagt
     8641 taccaactgc agtaagatat gttgtaaagc agaaaagcag caatactagc tagctaagct
     8701 caattttttt gcataggata acattttttt gtattgatta atttttttgt tactttaggg
     8761 tactaaatgg cggtatggga atgacatcaa atgaagcggc aaagcatgta aatctggaga
     8821 tcttaaagga gatttccgat ggaaatactg atttaatggt cgatttaatc aacctattta
     8881 ttggtcaagt aagaacattt agcgatcaac ttgaactcta caacaggaag aaggattatg
     8941 agtgtatggc gaagttggcg cataaaatta agaactctgt agccatgatg ggtattgacg
     9001 agctttcctc cgacatgaaa aaactggaac gcctggctca ggagggcaag aaaacaaact
     9061 tgtatgataa ttttattgaa agatttaaga aaatatcagg cgaggcagtg attgagctca
     9121 aggcaatcct caatagtctt taaccctaaa accttacaat catgatcgag gttgagaaaa
     9181 ttaacgatgc aatggttatt tcctttggga gtgataataa gctcaacgtt accgtcaccc
     9241 aaaagattaa aaccgagatt atcaagctga ttgatccaaa ctccaaaatt attttaaacc
     9301 taagcggcgt gagctacatt gacagtactg gttttggcat gctactctcc attctcagac
     9361 actgcaagaa ttccaactcc cagctaaagc tctgcaacat atcgcccgag gtaatggagc
     9421 ttataaagct actgcagctc caaaacattt ttgacatccg cgagggggtt aacgaaagcc
     9481 ttaaaagctt ctgctagcta ataccccctg ttaatttgta atataccttt ggagcaatat
     9541 gctttatatc gcttgtaaat aatagcttct cgttaaataa acctgcatta tacaaaaggc
     9601 gaagcagtgt tgcttcgccc tttgtatggc ggggtaaacc ctactccatg aagtcgtttg
     9661 tttggtccat ttcagcgtcg ttaacctttc gggctttata gttattaatc ttataggaga
     9721 acccgacaaa cagcatctgg ctatttctcc aattttcgct gtaggaggta aagttggtgt
     9781 cccaggagtt tgatctagat ttctgagtgt taaaaatatc gctagcccta acagtaatgg
     9841 tacctttgtt attaagtacc gatattctgg cacccaaatc aacccagtac atctccttgg
     9901 tttttccctg cgccccaccg attgaatgaa acccgttacc ggttccacta gccgagagaa
     9961 ttggtgacct gtagttaccg tttacctgaa tgtcaatttt tttcttaatg ttgaaaaatg
    10021 aggtgagctt agctgtccat gccgttccct catcggcgcc attaacaata tcgccataaa
    10081 gcttagtctt aaagtagcta tagctggcat tcagccgcca ccatttagtc aggttctgag
    10141 taaccacccc ctcaaccccg taacttttat tatgtgacag gttaataaaa gtagaaaggg
    10201 tggtaaccga gtcaaccaag gccagcttcc gggaaaatcc gttttgggtg tcgcggtagt
    10261 aaaccgaagc gcttaccttg gttgaaggaa agttaagtcc gtagttaaaa tcgagcgatg
    10321 tggtgaactc gggttcaagg taggggttac ccattgacca gttgtactta tccgcagtgt
    10381 taaggaacgg gttgagtatc ca
//
LOCUS       DAOPHX010000093        10275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_7979_length_10275_cov_5.979550, whole genome shotgun sequence.
ACCESSION   DAOPHX010000093 DAOPHX010000000
VERSION     DAOPHX010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 10275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10275
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7979_length_10275_cov_5.979550"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..273)
                     /locus_tag="PK502_07665"
     tRNA            complement(200..273)
                     /locus_tag="PK502_07665"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(237..239),aa:Met,seq:cat)
     gene            569..979
                     /locus_tag="PK502_07670"
     CDS             569..979
                     /locus_tag="PK502_07670"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HPH00732.1"
                     /translation="MKNDEGNGVYEVKLEYRVNTTPNILFQRLFTPYGLSEWFADDVN
                     LLGNTYTFIWNGSTRKAELVEKKENKYIKFRWIEAEGNSKTNYHFTFQLNRDDITNEL
                     SLLVTEEIEDLEERDEIISLWNYQINELKKAIGA"
     gene            1066..2523
                     /locus_tag="PK502_07675"
     CDS             1066..2523
                     /locus_tag="PK502_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPH00733.1"
                     /translation="MKTLHRFVLKSYLGPLVLTFFIVMFTLLMQFLWKYIDDLVGKGL
                     DGSVITELLLYASAGLVPMALPLATLLAALMAMGNLGENNELLAMKSAGISLPRILEP
                     LVVVIILISVGAFFFSNNVLPYTNLKMSTLLYSVKQQKPELIIKEGVFTNLIEGYSIK
                     VAEKDTKTNLMHRILIYDHSSDEGNNTVTYADSGYMKPTGDQKFLIATLYNGYSYKEV
                     KEKSGSRRNPSTNYPAQKQYFEKQVIVFELKGFDLQRTDESFFKDSYQVLNLSQLEKA
                     EDSLSTALNLQLESFSQNLTQNSIMRNPNWLKRTGSSPVSYKLDFDSLFSKLSPEQKQ
                     MAIARALDNARSTKIFVSNSKDEFKYRQKYISRHKIEWNRKFTLSFACFVFFFIGAPL
                     GAIIRKGGLGMPVVISVLFFIAYYIITITGEKFARELVWSPGLGMWVSSFILLPLGIF
                     LSYKATNDSSLMNADFYTEKLKKILRLGRKAKSNK"
     gene            2571..3776
                     /locus_tag="PK502_07680"
     CDS             2571..3776
                     /locus_tag="PK502_07680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPH00734.1"
                     /translation="MRILVLAHKPPFPPVDGGTLATLNMCIGLVKAGNTVTVLTLSTP
                     KHPSSIERIPESLLSEINFEILSIPLTTTPFGYFYNILFTRRPYNIQRYFSKTFKRII
                     LKTLRSNEFDIVQLEGLYLYPYVKTIRKHFSGKIVLRAHNVEHHIWETLANEESNKLR
                     AWYFNLLARRIARIERHINSNIDALVAISDPDYKWFTTNGLSKPAVVCPAGYFPPQQP
                     LESTPPDKPSLCYIGALDWMPNIEALMWFIDWVWPRIQSEIPEVEFYVAGRNATSELA
                     ERLMMERNIVFYGQVADSATFLSAYPVMIAPLFSGSGIRVKIIEGMFLRRAIVATSMA
                     SRGIKAVNNEHLLIADAPDEFAQAVCRLLSSPETASYISKNAQEFAFSHFDATHLAKE
                     LTNFYQRLI"
     gene            3776..4963
                     /locus_tag="PK502_07685"
     CDS             3776..4963
                     /locus_tag="PK502_07685"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPH00735.1"
                     /translation="MATSIWIFWLLAFILLYTYAGYTFLLWITAKIYSWTRSTATNME
                     SSAEELPAVTMVVAAYNEKAVVSQKIANINQLNYPPEKVFQLWVTDGSTDGTEEVLKD
                     YPEVSIIHHTERLGKAEAINRAMKLVQTPVTVFSDANTIVEPNSLIELVKPLTDSKTG
                     GVAGEKRILNSKNGNPTSAGEGIYWRYESFIKSMESATGSTISAAGELFAIKTELFTP
                     IPPDTLLDDFEISSRIILGGNRVKYSRYAAASEYGSLNFSEEMKRKVRIAAGGFQFLF
                     RNLQLLNPFPHPGYTFKYFSHKVLRWAFAPACLFLLPFFNAFIIAAESENPFYLLTLV
                     ILLSFFLLGLFGHLARNRKAIPHLMFLPYYAILMNISEIVGFFKFIQHKHNPKWDKSK
                     RET"
     gene            5036..6274
                     /locus_tag="PK502_07690"
     CDS             5036..6274
                     /locus_tag="PK502_07690"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPH00736.1"
                     /translation="MIKEDNSSIKLNTIEEALEEIRKGKVIIVVDDEDRENEGDFIAA
                     AELITPEIINFMAKHGRGLICAPLPEKRCDELELDLMVDRNTAPHNTAFTVSVDLLGH
                     GCTTGISASDRAKTVKALVNPSTTPEDLGRPGHIFPLRAKDKGVLRRPGHTEAAVDLT
                     RLAGLTPGGVLVEIMNDDGTMARLPQLLEIAKKFDLKIISIKDLIAFLLHSETIIERG
                     FKVKLPTEFGYFDLIPFRQKSNGLEHVALIKGEWEADEPILVRVHSSCVTGDIFGSYR
                     CDCGPQLHRAMQMIEHEGKGVLVYLNQEGRGIGLFNKIHAYKLQEEGLDTVEANIELG
                     FQPDERDYGVGASILHEVGVSNMKLITNNPKKMRGLEGYGLKIVENIPLVIPSNPFNE
                     VYLKTKVEKMGHINLFGPKQ"
     gene            complement(6324..6902)
                     /locus_tag="PK502_07695"
     CDS             complement(6324..6902)
                     /locus_tag="PK502_07695"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPH00737.1"
                     /translation="MPGVEAQLLMSPSIRKNVNGNSSSNGQTRQSAVLILFFPDEDET
                     RLVLIKRQTYEGLHSGQVSLPGGKWEEVDGSLEQTALREAFEEVGVNPERVELVGRLT
                     PLFIPVSNMMVQPVVGFTPEKPDFHPNLQEVDYIITVPVEQLFDQKNKSVRVISVNGS
                     PITAPYYNVNGEFVWGATAMILAELEAVASRK"
     gene            7126..7896
                     /locus_tag="PK502_07700"
     CDS             7126..7896
                     /locus_tag="PK502_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00738.1"
                     /translation="MKKIILISTLALLVASTGCVSKKYLKRGIKYEEAGLWELAAESY
                     IQSLTSKRDNLDAIVGLKRTGQKVIDEKCYKSIAAYESDNLKEAVYDYLDAVDFSKRT
                     SSLGVELMVNSRATDYFKDAKPRYIETLYAQAQQQMDAEKFQQAEALLDEIKKLEPDY
                     GNVKEMLKVSKCEPLYRQGKQLMESGYYRKSYGNFNQIVTEFGSYKDAAMLMGDALQK
                     AIITIKVNNFTGGYCTKALTQRIQSSVVASVNTLKPLR"
     gene            8015..8503
                     /locus_tag="PK502_07705"
     CDS             8015..8503
                     /locus_tag="PK502_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00739.1"
                     /translation="MLECKQDAGRLNKSDKRGYLKEVTKVKNPVTGETENKVEYKKVS
                     YEVYSIQNRVTFSLKFQLTSTETGAVMVSDIARSQQADETTYAVFNGKPEKLVPGYWE
                     NINKDSPKDFVSDNPTDVSALQKMLQARREISSVDQLTDKAIAALSKQVSERINRYNP
                     EQ"
     gene            8509..9900
                     /locus_tag="PK502_07710"
     CDS             8509..9900
                     /locus_tag="PK502_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HPH00740.1"
                     /translation="MVKNSYSTIALASIILLLAACSGTKKSVDTIPSPSWVQNRPTSS
                     IYYVGIGSAPKIGSPNDYQQAAKQNALADMASEISVKISSNSVISAFETQQNFYEDYS
                     ASIRAEAQKELEGYEMAEAWEDSQNYWVYYRLSKETYRKISDEKKAKAASLSLDYYDK
                     ALKSAENGDYRTCLLQVVRALETLKPYFAESVTASYQGREIFLGNELIQLLTETLNNL
                     LLNGPSEVMARMGQPIPAGSLTYSVTNRIGIPQKGMPLRASYSEKPMLMNKSITGSDG
                     KACFSVDRVSSSRNVEKIAVVLDFSAIVQEATTDFDLRKLLSRFQVPSVETSIRIDKP
                     TFSVVTREMLFDKPAQKNVLAEAIKRKVTDEGFPIAEKTSDADFAITIEASTAKTGES
                     GSYKQASLSALIYVTNAQGVEVYRRSLDPITGSHFDWEAAGLSTFDKAAKRLESSIVP
                     EIIEVVVLGRSSY"
     gene            complement(10052..>10275)
                     /locus_tag="PK502_07715"
     CDS             complement(10052..>10275)
                     /locus_tag="PK502_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPH00741.1"
                     /translation="ALIDRIVRVANDEAFAMVRRLAREEGMLVGISTGANLVAVEKII
                     AGVEKSISILTFAYDSAERYLSIDDLWQE"
BASE COUNT         3009 a         2498 c         2211 g         2557 t
ORIGIN      
        1 ggcagcacgc tgcccttgct tatggcttct tccagatttg caaccacccg caacatccct
       61 gctattcgct atcgcttact cccaagccgg cgtgtgcaag aaattgcatc ttgccggtag
      121 tgtagtgtag tagtgtttgc ccgacataca aaaaaaaaca cttacatcgt tttatgatat
      181 aagtgcttga tttttagagt agcgagggcg ggaattgaac ccgcgacctc atgattatga
      241 atcatgcgct ctaaccagct gagctacctc gccctaaagc atttatcgct gattattctc
      301 ccttaatctc agcacaccca tgcctagttc caggaacgta ttaactagtc atgaaaaaac
      361 atgccaagac ccacatttgt gccgcaaaag tagaaaataa ttcaatacct caaaaaaatg
      421 ttcggtaaat atttagcatc aacccgcaac tttgcggttt agaaaatggt tatcaacacg
      481 aattgttaat aaataatatc caggagctta ggcaaaatct tcctaaaaag ttatattgca
      541 agtctagttg aagtaaacaa tggagaacat gaaaaacgat gagggaaatg gggtatacga
      601 ggtgaagctg gagtacaggg ttaacaccac cccaaacatt ctattccaaa ggctttttac
      661 cccctacggg ctgtcggagt ggtttgccga tgatgttaac ctgcttggca acacctacac
      721 gttcatctgg aatggttcaa cccgaaaggc cgagttagta gaaaaaaagg aaaacaagta
      781 cattaagttt cgttggattg aggccgaggg gaactccaaa acaaactacc acttcacctt
      841 ccagctgaac agggatgata ttaccaacga gctttcgctg ttggttacag aagaaattga
      901 agatttggag gaaagggatg aaattatctc gctttggaac taccaaatta acgagctaaa
      961 aaaggccatt ggcgcataat gatagagttg aattgcctcc ggtagagcta tttctgggaa
     1021 aatggttacc tttgcccttg tttttaacca tatgagggca aattcgtgaa aacactgcat
     1081 cgttttgttt tgaaatcata ccttgggcca cttgtgctca ccttctttat tgtcatgttt
     1141 accctgctca tgcagtttct ttggaaatac attgacgatt tggtaggtaa aggccttgat
     1201 ggaagtgtga ttaccgaact attgctctat gcttcagcag gtttggtacc catggcgctg
     1261 cccttggcaa cgcttttagc agcattaatg gccatgggca acctgggcga aaacaacgag
     1321 ctgctggcca tgaaatcggc ggggatatca ctccctcgaa ttttggaacc gctagtagtt
     1381 gtcattatcc tcatcagcgt aggcgctttt ttcttttcta acaacgtgct tccctataca
     1441 aaccttaaaa tgtcaaccct tctatactcc gttaagcagc aaaaaccgga gttgattata
     1501 aaggaaggcg ttttcacaaa ccttattgag ggttacagca tcaaggtggc tgaaaaggac
     1561 accaaaacta accttatgca ccgcatcctc atatacgacc acagctccga cgagggcaac
     1621 aacacggtta cctatgccga ctcaggctac atgaagccca caggcgatca gaaattttta
     1681 attgccaccc tttacaacgg gtattcctac aaagaggtta aggaaaaatc gggctcacga
     1741 agaaatccca gcaccaacta ccctgcccaa aaacaatatt ttgaaaaaca ggtgatagtt
     1801 tttgagctca agggatttga ccttcagcgc accgatgaga gctttttcaa ggacagctac
     1861 caggtgctaa acctttcaca actcgaaaag gcagaggatt cgctctccac cgccctaaac
     1921 ctgcagcttg aaagtttttc ccagaacctt acccaaaaca gcatcatgcg aaatcctaac
     1981 tggcttaaga gaaccggttc ttcgccggta tcctacaagc tggattttga ttcgctgttc
     2041 agcaagctat caccggaaca gaaacaaatg gccattgcca gagctctcga caatgcccgc
     2101 tccaccaaaa tattcgtatc caactcaaag gatgaattca agtacaggca aaagtacatc
     2161 agccggcaca agattgaatg gaacaggaag ttcacgcttt cttttgcctg cttcgtattc
     2221 tttttcattg gagctcccct gggggcgata atcagaaagg gtggattggg tatgccggtg
     2281 gtaatttcgg ttcttttctt tatcgcatac tacataataa ccattaccgg agaaaagttt
     2341 gcccgagagc tggtctggag tcccggcctg ggcatgtggg tttcatcatt catactgcta
     2401 cccttgggaa tctttctatc ctacaaggct acaaacgact cttcgctaat gaatgccgat
     2461 ttctacaccg aaaaactcaa aaagatactt cgacttggcc ggaaggcaaa aagcaacaag
     2521 taaccacagg caattaacat ctccctcata ggagctatat tcataaggca atgagaatac
     2581 tggttctagc gcataaaccg ccattccctc cagtggacgg tggaactctg gccaccctta
     2641 acatgtgcat aggacttgtg aaggcaggaa acacggtaac cgtactcacg ctcagcaccc
     2701 ccaagcaccc cagctctatt gagcgaatcc cggaatctct tctctccgag attaacttcg
     2761 aaatcctgag cataccgctg accacaaccc cttttgggta tttttacaac atcctgttca
     2821 cccgaagacc atacaatatc caacgatact tcagcaaaac gtttaaacga ataattctta
     2881 aaacccttcg cagcaacgag tttgatatag ttcagctgga gggactctac ctctacccct
     2941 acgttaaaac catccgaaag cactttagcg gtaaaattgt tttgcgggcc cacaatgtag
     3001 agcatcatat ttgggaaact ttggctaacg aggaaagcaa caagctaagg gcatggtact
     3061 tcaacctcct ggctcgacgt atagcccgga ttgaaagaca tataaacagc aacattgatg
     3121 ccctggttgc catcagcgat cccgactaca agtggtttac tactaatggc ttaagcaaac
     3181 ccgctgtggt ttgtcctgcc gggtattttc caccacaaca gccactggag tcaacccctc
     3241 ccgataaacc atcactctgc tacattggag cgcttgactg gatgcccaac atcgaagccc
     3301 tgatgtggtt catagactgg gtttggccaa ggatacagtc cgaaattccc gaagttgaat
     3361 tttacgttgc cgggcgaaac gccaccagcg agttggccga gcggcttatg atggaaagaa
     3421 atattgtctt ttacggacag gttgccgatt cggccacatt cctcagcgcc tatcccgtta
     3481 tgatagcccc gttgttctcg ggaagtggaa tacgggtgaa aattatcgag ggcatgttcc
     3541 tccgaagagc aattgtggca acaagcatgg cttcaagggg tataaaggct gttaacaacg
     3601 aacacctact catagccgat gcccccgacg aatttgccca agctgtctgc aggctattaa
     3661 gctcacccga aactgcaagt tacatctcta aaaatgcaca ggaatttgcc ttcagccatt
     3721 ttgatgcaac ccacctggcc aaggagctta ctaattttta ccaacgcttg atttgatggc
     3781 tacgagcatc tggatatttt ggcttctcgc cttcatccta ctttacacct atgccgggta
     3841 cacctttcta ctctggatta ccgcaaagat atactcatgg actcgttcca ccgcaaccaa
     3901 catggagagt tcagccgaag agctgcctgc ggtaaccatg gttgtggctg cctacaacga
     3961 gaaagctgtg gtgtcgcaga aaattgccaa catcaaccag ctaaactacc caccggaaaa
     4021 agtcttccag ctatgggtaa ccgatggttc aactgacggg acagaagagg tgctaaaaga
     4081 ttacccggaa gttagcatca ttcaccatac cgaaaggttg ggtaaggctg aggctatcaa
     4141 cagggctatg aagctggtgc aaaccccggt taccgttttt tcagacgcca ataccattgt
     4201 tgaacccaac tcgcttattg aacttgtaaa accgctcacc gactcaaaaa cgggtggagt
     4261 tgccggtgag aaaagaatac taaacagtaa gaatggtaat ccaacctcag ccggtgaagg
     4321 gatttactgg aggtacgaat catttattaa aagcatggag tcagcaaccg gatcaaccat
     4381 aagcgcggca ggtgagctct ttgccataaa aaccgaactt tttaccccaa taccccccga
     4441 cacgctgctt gacgattttg agatttcttc acgtattatc ctgggtggta accgggtaaa
     4501 gtactcaaga tatgcagctg ccagcgagta tggttcgctc aactttagcg aggagatgaa
     4561 gcgtaaagtc agaattgctg cggggggatt tcaattcctt tttcgcaacc tgcagcttct
     4621 taacccattc ccccaccccg gttatacctt taagtatttt tcccataagg tgcttcgctg
     4681 ggcgtttgct ccagcttgcc tttttctcct tccttttttc aacgccttca tcattgcagc
     4741 cgaatcagaa aatccgtttt acctgctcac cctagtaatc ctgctctcct tttttctgct
     4801 gggtctattt ggccatttgg cacgaaacag aaaggcaata ccccacctca tgttcctacc
     4861 ctactatgca atcctaatga acatttcaga aattgttggg ttctttaagt ttattcaaca
     4921 taagcacaac cctaaatggg acaaatcgaa aagagaaaca tgacaattgt ccggaatatt
     4981 tattgctcca aaaagctact tttgcaaacg agtttcgaaa aatacacatc aagaaatgat
     5041 caaagaggac aatagctcca taaagctcaa caccattgaa gaagcgctgg aggaaattcg
     5101 taagggtaaa gttatcatag tggtggatga cgaagacagg gagaacgaag gcgatttcat
     5161 tgcggcagca gaacttatta ctcccgaaat tatcaacttc atggccaagc acgggagggg
     5221 tttaatttgt gctcctctac ctgagaaacg atgcgatgag ctggaactcg accttatggt
     5281 tgataggaac actgcccccc acaacaccgc tttcaccgtt tctgttgact tgcttggaca
     5341 tggttgcacc acagggatat cggcatccga tagggcaaaa accgtcaagg ctcttgttaa
     5401 ccccagcaca acccctgaag atttgggacg cccaggccac atatttcccc tcagggctaa
     5461 ggacaaaggc gtacttagaa ggccaggaca taccgaggcg gccgttgacc tcacccgttt
     5521 ggcagggtta acaccgggag gagtgctggt ggagattatg aatgacgatg gaactatggc
     5581 cagactcccg cagctgctcg agattgcaaa aaaattcgac ctaaaaataa tttccataaa
     5641 agaccttatt gccttcctgc tccattccga aaccatcatc gagagaggtt tcaaggttaa
     5701 acttcctacc gagttcggct acttcgacct tattcccttc cgccagaaat caaatggact
     5761 tgaacatgtt gccctaatca agggggaatg ggaggccgac gagcctatac tggtaagagt
     5821 ccactcctcc tgtgttactg gtgatatatt tggctcctac agatgcgact gtggaccaca
     5881 gctacaccgc gccatgcaga tgattgaaca tgagggaaaa ggagttttgg tttacctaaa
     5941 ccaggagggc agaggaattg gcctgttcaa caaaattcac gcctacaagc tacaggaaga
     6001 agggctggac accgttgaag caaatatcga gctgggcttt caacccgatg aacgcgatta
     6061 tggcgtagga gccagtatcc tccacgaggt tggtgttagc aacatgaagc tcattaccaa
     6121 taatccgaaa aagatgaggg gtctcgaggg ttacggttta aaaattgttg aaaatattcc
     6181 cctggttatc ccatcaaacc ccttcaacga ggtgtactta aaaaccaagg tggagaaaat
     6241 gggccatatc aacctttttg gacctaagca gtagcctctc caaattaatc taccactacc
     6301 tccaagccaa aagccccata gacctacttt cgggaagcaa ccgcttcaag ttctgccaga
     6361 atcatggctg ttgcacccca cacaaactcg ccattcacat tatagtaggg agctgttatg
     6421 ggtgaaccat taaccgatat aaccctcacc gatttatttt tttggtcaaa aagctgctcc
     6481 accggtacgg ttatgatgta gtcaacttcc tgtaggttgg gatgaaaatc gggtttctcg
     6541 ggcgtaaacc ccaccactgg ctgcaccatc atgttgctaa ccggaatgaa caaaggcgtt
     6601 agcctgccaa ccagctccac cctttcagga ttcacaccta cctcctcaaa tgcctcccgc
     6661 agagcggttt gttccagcga tccgtcaact tcctcccatt tgccacccgg aaggctcact
     6721 tgtccgctgt gaagcccttc gtaggtttgc cttttgatga gaaccaacct tgtctcgtct
     6781 tcgtccggaa agaataggat tagcactgca ctttgacggg tttgcccatt tgagctgcta
     6841 tttccattca cgtttttacg aatggagggc gacatcaaca gctgtgcctc tacaccaggc
     6901 aaaccttggc gcattttacc cttcaaactg cttataaaat gagaaaacgc acatgcagaa
     6961 aaactataca tagcctgcta agctccttaa aatatcattc cactacaacc tatcaaaggt
     7021 aaaaaaattg aatttatcat tacccaacat tgcgggtata tcatatttcc ttaactttac
     7081 gctagaaagg tttttccata ttggttcata ttagtgcagc taaatatgaa aaagataatt
     7141 ttaatttcga ctctggccct gttggttgcc agcacagggt gcgtatctaa aaaatacctc
     7201 aagcgaggca ttaagtatga ggaagctgga ctttgggagc tagcagccga atcgtacatc
     7261 caatcgctaa cctcgaaacg tgacaacctg gatgcaattg ttgggcttaa acgaacaggt
     7321 cagaaagtga ttgatgaaaa gtgctacaaa tcaatagccg cttacgaatc ggacaatttg
     7381 aaggaagctg tgtacgacta ccttgatgcg gtggatttta gtaaacgaac atcctcgcta
     7441 ggcgttgagc taatggttaa tagtagggct accgattatt tcaaggatgc caaacctaga
     7501 tatattgaaa cgttgtatgc tcaagcccag cagcagatgg atgctgaaaa gtttcagcaa
     7561 gctgaagccc tgcttgatga gattaagaag ctggaacccg actatggaaa cgtgaaggaa
     7621 atgctgaagg tatctaaatg tgagccctta taccgacagg ggaaacaact gatggaaagt
     7681 ggttactacc gaaaatccta cggaaatttt aaccagatag taaccgaatt tggcagctac
     7741 aaggatgccg ccatgctgat gggtgatgcc ctccaaaaag ccattataac cataaaggtc
     7801 aacaatttta cagggggtta ctgcactaaa gccctgaccc aacgcataca aagttcggtg
     7861 gttgcttcag taaataccct taaacccctt cgttaaaatt gttgactccg ataataccat
     7921 acaaatcatc gaggaacaac aacgtagcct tacacaaggc tcggacattg aagttggtaa
     7981 aatactggct gcaaaagcaa ttctaacagg cgaggtgctt gaatgtaagc aggatgctgg
     8041 ccgtctgaat aaatccgaca agcgaggcta ccttaaagag gtaaccaagg tgaaaaatcc
     8101 cgtaacaggt gaaacggaga acaaggtgga gtataaaaag gtttcctacg aggtttacag
     8161 cattcagaac cgggtaactt tctcgctgaa atttcagctt acatcaaccg agaccggtgc
     8221 cgtgatggtt tccgatattg cccgaagcca gcaagcagat gaaaccacct atgctgtatt
     8281 caacggcaag cccgaaaagc ttgtccccgg atattgggaa aatattaata aggattcacc
     8341 aaaagatttt gtatccgaca acccgaccga tgtttctgca cttcaaaaaa tgctacaggc
     8401 ccgcagggaa atatcctcag ttgaccagct aaccgacaag gccattgctg ccctgtccaa
     8461 acaggtttcc gaacgaataa accggtataa tcccgaacag taaaaactat ggttaaaaac
     8521 agctactcga cgattgctct tgcctccatc atattgttgc tggctgcatg tagcggaaca
     8581 aaaaaatctg ttgatacaat tccgtcccca tcgtgggtac aaaatcgccc aacctcctca
     8641 atttactacg ttggtatagg ttctgcccct aaaataggca gcccaaacga ttaccaacaa
     8701 gctgccaaac aaaatgcact ggctgatatg gcaagcgaga tttcggtaaa gatatcgtct
     8761 aactccgtga taagcgcctt tgaaacccag caaaattttt acgaggatta ctcggcaagc
     8821 atacgtgccg aagctcaaaa ggaacttgaa ggatatgaga tggccgaagc atgggaggat
     8881 tcacaaaact actgggttta ctaccgcctt tcgaaagaaa cctaccggaa aatttcagat
     8941 gagaaaaagg caaaagcagc ttcgctctcg ctcgactact atgataaagc attgaaaagt
     9001 gctgaaaatg gtgattacag aacctgcctg ctccaggtag ttagggcact ggagactctc
     9061 aaaccatact tcgccgaatc ggttactgcc agctaccagg gaagggagat ctttctggga
     9121 aatgagctaa tccagctgct taccgaaacc ctgaataacc tgctgcttaa cggaccttcc
     9181 gaggttatgg cacgaatggg acaaccaatt cctgccggct cgttaaccta cagcgttacc
     9241 aatcgtattg gtattcccca aaagggaatg cctcttaggg caagctactc cgaaaagcca
     9301 atgctgatga acaagagtat taccggtagt gatggcaaag cttgtttttc ggtcgatcgt
     9361 gtcagctcct cacgcaatgt cgaaaaaata gccgttgtgc tggactttag cgcaatagtt
     9421 caggaggcca ccaccgactt tgacttgcgt aagcttctat cccgatttca ggttccttcg
     9481 gttgaaacct caataagaat tgataagcct acattttcgg tagtaaccag ggaaatgctg
     9541 ttcgacaagc ccgctcaaaa gaacgttctg gccgaagcca taaagcgtaa agtcaccgat
     9601 gagggattcc cgatagccga aaaaacatcc gatgcagatt ttgccattac cattgaggcc
     9661 tcaactgcta aaaccggaga atcgggcagt tacaagcagg caagcctttc agctttgata
     9721 tacgttacca acgcacaggg tgttgaggtt taccgccgaa gtttagaccc cataaccggt
     9781 tctcattttg actgggaggc tgctggctta agcaccttcg acaaagcggc caaacggctt
     9841 gagagctcta tcgtccccga aataattgaa gtagtagttc tgggcagatc gtcctactag
     9901 ccaattccat aaggtttact tacaatccga tttgcttacc tacttttacc aatggattaa
     9961 atcatttaac agcccgatca atgattgtct gcatgcaata atccaaagag tatgcatgaa
    10021 aaacacagca tgaacatgcg gtttacatcc gctattcctg ccataggtca tctatactca
    10081 gatacctctc tgcactgtcg taggcgaacg ttaatatgct aatagatttt tcaactcctg
    10141 caataatttt ctccactgca accagatttg ccccggttga aatgccaacc agcatgccct
    10201 cttctcgcgc tagtcggcgc accatggcaa aggcttcatc attcgctacc ctaacaatcc
    10261 tatcaataag cgcta
//
LOCUS       DAOPHX010000094        10267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_7989_length_10267_cov_6.416960, whole genome shotgun sequence.
ACCESSION   DAOPHX010000094 DAOPHX010000000
VERSION     DAOPHX010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 10267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10267
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7989_length_10267_cov_6.416960"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1885)
                     /locus_tag="PK502_07720"
     CDS             complement(<1..1885)
                     /locus_tag="PK502_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPH00742.1"
                     /translation="MKEFVTACPRNCYSTCSFIVQVEKDKVVGIKPLSANKATPTGVC
                     LKGLSYVERANSPDRILYPYKKNGSGDFIRISWDEALSTIAGKLRYFKKTFGSQSVLF
                     YAASGMSGLINEFSGRFWREIYGGVTTTYGNLCWPAGLEATRLTLGENKHNAPWDLEN
                     ARLIVLWGKNPAESNIHEMIPLSTALDKGAKLAVVDPRRTSSSQRADLLVQPLPSTDA
                     ALALAVANILIKNSWIDEDFISSYVNGFEEFKAKAMSCTPEWASSICDISVESIEKLA
                     WLIGNVKPMTIIPGYGMQRYTNGGQTIRAILALSVITGNIGKPGACWHYANLQSYVFD
                     EVLEPICYYPPEEPDSTTRRSISTALLGEDMLSTTNPQLKMIWVERGNPLTQNPNTGS
                     VLEAFRKLDFRVVVEQFFTDTALEADIVLPAKNMFEQSDIIGSYWNPYIQLKQKVVEP
                     AGEVKPETEIYWLLAKQLGFNENTLKNILLPPGNDEVEKFLAQKLAKFPGLSLELLKM
                     GPVVPPGHQEIAFSDFRFATPSGKIELLSEQVASLWNANPLPEFLPAMEISDANSTYP
                     LLLLTPNTKNRIHSQFGNLKIIKFINGNPVVQMNPSDAMHRGIKNGSLVKVYNARGSI
                     QVSA"
     gene            complement(1947..3560)
                     /locus_tag="PK502_07725"
     CDS             complement(1947..3560)
                     /locus_tag="PK502_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00743.1"
                     /translation="MPVSSKGVLELTLDYDCDYIFSFEKEGYISKKIAIDTRIPDKLK
                     ESFDKVTAFDVELFKQSENEVKEYKNPVARIQFSSQISDFVFDTDYSLRVQQEIAQAN
                     KDLVEERKKQESLDLAKKEEEKRQQLLAEAQKRVEAEAQKKAAEEKARQVEAEQLKKE
                     AEERRLAEAKAKEEALKREEELRLKAREEARIKAEQEALRIAEQKRIEEEKLKQEQER
                     IRQEEEAKRKAAEEARIKEEQRIREEAERIRVAEEAKKKADEEARLKELEQQKKEAEE
                     RRLAEAKAKEEALKREEELRLKAQEEARIKAEQEALQIAEQKRIEEEKLKQEQERIRQ
                     EEEAKRKAAEEARIKEEQRIREEAERIRVAEEAKKKADEEARLKELEQQKKEAEERAI
                     ADAKLKEEQRRIELEKAQKEAERKKFEEEERLKAEQQQKLLFEEQKKRDKELRLKLVA
                     EKMSAKIGTEDAERVVTLLTPEDVTKKYTRERTDEVIRLPRIVITRTVYNRNDKITFY
                     TRVDHEYGAIFYFKDGYIISEEQYKAETK"
     gene            3956..4630
                     /locus_tag="PK502_07730"
     CDS             3956..4630
                     /locus_tag="PK502_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPH00744.1"
                     /translation="MPMCFSINLNIEKYALEKSYHAKFSASYGYTPFYFASAFENPMV
                     PVIANRDSSLIVPMVWGLIPHWVTTPEQANLIRSKTYNARIETIHEKPSFRYAARSNR
                     CIIPVTGFFEWQEVNGKKVSWLIKPRADATFSIAGLWNSWTNVETGELIDTFTIITQP
                     ANELMSQIHNTKKRMPAIVDKDLVKSWLDAKLPTLKYDAILRPLDSELMDAHVVPSPL
                     RRPKSQ"
     gene            complement(4703..5776)
                     /locus_tag="PK502_07735"
     CDS             complement(4703..5776)
                     /locus_tag="PK502_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00745.1"
                     /translation="MKKLVKLMMAMLVLAFAFASCGGDDETQYAEFENGAIILNEGAY
                     GNSNASITFIGYGSDIADNQIFYRINGRELGDVLQHAAIYNGKIYMVLNNSGKVEVAS
                     SATFIQLQTITGLVNPRYVAAKDGKIYVTQWGSWGEQGTVEVFDANSFSHSNTIKVGT
                     GAEGLLCTDGKIWVANGGGYGLDSTVMVVDAATGAVSDTVTVGYNPKELVEDASGNIW
                     VICSGADIYDDSWEVVGHLPSKLVMIDRQSRQVVHSLIISNTSHPAHIDVSSGKETVY
                     FGGGFGVNGIFSVSITQPVIPQTPVINGFFYGFNIRPSNGDIYCMVAPNFTSAGELKI
                     YSSAGAALKSYSVGIAPSTALFN"
     gene            complement(5805..7655)
                     /locus_tag="PK502_07740"
     CDS             complement(5805..7655)
                     /locus_tag="PK502_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPH00746.1"
                     /translation="MSLSDTIRMDEVKVYGNLRESHSIGATIYRMDSVKLKIFQNRSA
                     AELLAMGSVNLKSYGVGGLSSLTMRGGNSSQTIVVWNGVNIQNPMNGGANLSLFPVNM
                     FSSIRLQYGGSGTIYGSGAISGILILNSNALFPQPNGGELSLSYGSGNTRSLSSQVKY
                     GDKSRAISVKVFGQLADNDFEFRNIYKIGSPKERITHAESKQLGILTDVNMRLHEKLF
                     WSFSGWGQLGDKNLQPIMSSTQPSYANQVDRSLALVSNLSFSPSKRILFNLKNAASLG
                     VIDYSDKGVNIFTNNSYASILTELESKVDVLNNGEVSMGINHTLETASSDDYVGSAKR
                     NRVSGYASLRKPLFSSRFLSVLSIRGELVDGDFKPVIYSVGAEWLPTKKLIFKMNASK
                     NYRIPTLNDLYWASTTYTEGNPNLEPESGWGGDVGFQVKSISSDRAINVSNSLFYSNI
                     ERWIVWLSIPTVSDSKWKPFNMSKGESWGWESQVDWSLRVNSLTIRVEGFYTFTHSVF
                     SEQPGSRAQPMIYTPTHRFSGSLNLQYEKYGLGYYHGFTGKRYRDFQSQLPAFNLANL
                     FISYKFNLLWKVVTAGLSVNNLWNQDYQLIAGYAMPLRSYMFTINIDFTR"
     regulatory      complement(7828..8015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8113..10194)
                     /locus_tag="PK502_07745"
     CDS             complement(8113..10194)
                     /locus_tag="PK502_07745"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPH00747.1"
                     /translation="MIVILVAASCKNNSSEVKNPLLAEFDTPFQTPPFDKIRHEHYAP
                     AIDSAIAEAKAEVEAITSNPAKSTFENTIEALDRSGRRLNLVSNILFNLNESETDSTL
                     QGIARDAAIKLTEFSNDVNLNPELFKRVKQVWEMRDSLKLTTEQQTLLSNTYKGFIRN
                     GANLSDSDKEKFRAISKELADLSLRFNENLLAETNSYQLHITNESDLAGLPASLKDAA
                     AYTAQQKGKVGWIITLDYPMYGPFMKYSENRELRKQLYMAYGSRCFKGNEYDNQAIAK
                     KIANLRLEIANLLGYPNYASFVLQDRMAENPERVNNFLKELVDASMPAARREVEEVRG
                     FAKELGFNENIQPWDWSFYSEKLKNSRYSYNEEELKPYFELNRVINGVFTLANKLYGL
                     TFTPNNQIPVYHPDVKAYEVYDATHKFMAVLYLDFFPREGKSGGAWMTSFRSQYRDGS
                     TDVRPFVSLVTNFTKPTATQPSLLTFYEVSTFMHEFGHALHEMLSECTYSSLSGTSVY
                     RDFVELPSQLMENWAVEKEFLDMFASHYQTGEKIPQELVQKIIDSRNFLAGYSSIRQL
                     SFGVLDMAWHTVEKPVTISVGEFEKRAIANLEVLPTVEGTNQSVGFGHIFDGGYAAGY
                     YGYKWAEVLDADAFEMFKEHGIFNPEVAASFRTNILSKGGSEHPMELYKRFRGHEPSI
                     DPLLRRSGLKN"
BASE COUNT         2634 a         2430 c         2139 g         3064 t
ORIGIN      
        1 gtgcactaac ttgaatacta ccgcgggcat tgtaaacttt taccaggcta ccatttttaa
       61 tgcctctatg catagcatct gatgggttca tttgcactac aggattgccg ttgatgaatt
      121 ttataatttt caggttgcca aattgggaat gaatcctgtt ttttgtattt ggagtcaaaa
      181 gcagtaatgg gtaagtacta ttggcatcgc ttatttccat ggcaggcaaa aattccggta
      241 gtggatttgc attccagaga gaagcaacct gttccgaaag caactcaatt ttacccgacg
      301 gggtcgcaaa gcgaaaatcg gaaaaggcaa tttcctgatg tccaggagga acaactggac
      361 ccattttcag taactctaat gataatccgg gaaatttggc aagcttctgg gccaagaatt
      421 tttcaacctc atcatttccg ggaggtaaaa ggatattttt aagagtattt tcattaaaac
      481 ccagttgttt agccagtagc cagtatattt ccgtttcggg tttcacctct cctgcgggtt
      541 caactacttt ttgttttaac tgaatataag gattccagta ggatccgatt atatccgact
      601 gttcaaacat gttctttgct ggtaaaacaa tatcggcttc aagggcagta tcggtgaaga
      661 attgctcaac aaccacccgg aagtctagct ttcggaatgc ctccaaaacc gatccagtat
      721 tgggattttg ggttaacggg ttgccccgtt ccacccaaat catttttagc tggggattcg
      781 tagtgctgag catgtcctcg cctaacaggg ctgtagagat ggaacgacgg gtagtgctgt
      841 cgggttcttc gggtggatag tagcaaatgg gttccagcac ttcatcaaac acgtagcttt
      901 gaagatttgc atagtgccaa caagcgccgg gtttaccaat atttcctgta attacagaga
      961 gtgcaagtat tgccctgatg gtttgccctc cattggtgta gcgctgcatt ccgtatccgg
     1021 gaataatggt cataggctta acattcccta ttagccatgc cagcttctca atggattcta
     1081 ctgagatgtc gcaaattgaa gaagcccatt cgggtgtgca gctcatcgct tttgctttaa
     1141 actcctcaaa tccattcacg tagctactaa taaaatcttc gtcaatccag ctgttcttta
     1201 tgaggatatt agccacagct agagccaaag cagcatcggt ggatggaagc ggttgaacca
     1261 gcaggtctgc tcgctgggat gaacttgttc ggcggggatc aaccacagca agctttgccc
     1321 ccttgtcgag agcagtgctt aaagggatca tctcgtggat gttgctttcg gctggatttt
     1381 tgccccagag tacaattagc ctggcatttt ccaaatccca tggcgcgttg tgcttgttct
     1441 cgcccaaagt taaccgtgtg gcttcaagcc cagctggcca gcataaattt ccataggtgg
     1501 tagttacacc tccgtatatt tcgcgccaga accttccgct aaactcgttt ataagtcccg
     1561 acataccgct ggcggcgtaa aagagaacac tttgcgaacc aaaggttttc ttgaaatagc
     1621 ggagcttccc tgcaatagtc gaaagtgctt catcccagct aattctgata aaatcaccac
     1681 tgccattttt cttgtaggga taaagtatcc tatcgggaga atttgccctt tcaacgtagc
     1741 ttaacccttt aagacatacc cccgttgggg ttgctttgtt tgcagataac ggtttgatgc
     1801 caacaacctt gtctttttca acttgaacaa tgaagctgca agtactgtag cagttccgtg
     1861 ggcatgctgt aacaaactcc ttcacgaatt attatagtta caatctaaaa aatgggaatt
     1921 tatttaccgg ttgagaatga attatgctac tttgtttcgg ctttatactg ctcttctgat
     1981 ataatgtagc cgtccttaaa gtaaaatatt gcgccatact catggtcaac gcgggtgtaa
     2041 aaggtaatct tatcatttcg gttgtaaacc gtgcgggtaa tcacaatcct tgggagacga
     2101 attacctcat cggttcgttc tcgggtatat ttcttagtaa catcttcggg ggtaagaagg
     2161 gttaccacac gttcggcatc ctctgttccg attttagcac tcattttttc ggcaaccagc
     2221 ttcaagcgca gctccttatc cctctttttc tgctcttcaa aaagcagctt ttgttgctgt
     2281 tcggctttta aacgctcctc ctcctcaaac ttttttcttt cagcttcctt ttgtgccttt
     2341 tccagctcaa tccttcgttg ttcttccttt agcttagcat cagcaatggc acgctcctcg
     2401 gcttcctttt tctgctgctc caactccttc agtctggcct cctcatcggc tttctttttg
     2461 gcctcttcgg ctacccgaat gcgctctgcc tcctcgcgta tgcgttgttc ctccttaata
     2521 cgggcttcct cggcggcttt cctttttgcc tcctcctcct gcctaatgcg ctcctgctcc
     2581 tgttttaact tttcctcctc aattcttttt tgttcggcta tttgcagggc ttcctgttcg
     2641 gctttaatcc tggcttcttc ctgtgccttt aaccttaact cctcctctcg ttttagagct
     2701 tcttccttag ctttggcttc tgccaagcgg cgctcctcag cttccttttt ttgttgctcg
     2761 agttccttca gcctggcctc ctcatcggct ttctttttgg cctcttcggc tacccgaatg
     2821 cgctctgcct cctcgcgtat gcgttgttcc tccttaatac gggcttcctc ggcggctttc
     2881 ctttttgcct cctcctcctg cctaatgcgc tcctgctcct gttttaactt ttcctcctca
     2941 attcttttct gttcggcaat acgcagggct tcctgttcgg ctttaatcct ggcttcttcc
     3001 cgtgctttta accttaactc ctcctcacgt tttaaagctt cttccttagc tttggcttct
     3061 gctaagcggc gttcctccgc ttcctttttc aactgttcgg cttcaacttg acgtgccttt
     3121 tcttctgctg cctttttttg ggcttctgct tccactctct tttgagcctc ggctaaaagc
     3181 tgctgacgtt tttcctcttc ttttttagcc aaatccaaac tttcctgctt tttacgttcc
     3241 tccaccaagt ccttgtttgc ctgggcaatt tcctgttgaa cgcgtaggga gtagtcggta
     3301 tcgaatacaa aatccgaaat ttgggaagaa aattgaatcc gcgctaccgg atttttgtac
     3361 tccttaactt cattctcact ttgcttgaaa agttcaacat cgaatgcagt aaccttatca
     3421 aaactttctt ttagcttatc gggtattcgg gtatcaatgg cgattttttt ggaaatatat
     3481 ccttcctttt caaaggaaaa aatgtagtcg cagtcgtaat cgagagtcag ttcaagtaca
     3541 cccttactgc taacaggcat ttgcctttca agattgccat ttttgtatat tgatatctta
     3601 gacccttgta attcgccctt tttaataatc aatcgggcag atatttgtaa gccgttttgt
     3661 gaaagcaata tgcttggaat tgctactaag catagggcaa gccatactat gagtatggtt
     3721 gatttggcct tttgtttcaa ttccgtaagc attaacccaa attttctaac aaaaaaagta
     3781 aaaaaatttg atattactac gaaaatttga gcaattctgt tcgcttcatc ggcatgactc
     3841 catagcatgg caagtacctg cttaagtatt ttcgtagtaa atgcaatcgt aaacctgatt
     3901 tcagaaatat tcgtttactt ttgctttaaa ggtagaaaat tgggtgattg aaaaaatgcc
     3961 tatgtgcttt tccataaatc taaatattga gaagtatgcg ttggagaaaa gctaccatgc
     4021 gaaatttagt gcttcgtatg gatatactcc attttacttt gcctcagcct ttgagaatcc
     4081 aatggttccc gtaatagcca atcgggatag tagccttatt gtccctatgg tttggggatt
     4141 aattccccac tgggttacca caccagaaca agccaacctg attagaagta aaacctataa
     4201 cgctagaatt gaaacaatac atgaaaaacc ttcgttccgt tatgctgcaa ggtctaacag
     4261 gtgcattata cccgttaccg gatttttcga atggcaagaa gtaaacggga aaaaggtgtc
     4321 ctggttaatt aagccaaggg cagatgctac attctccata gccggactat ggaactcatg
     4381 gactaatgta gaaacagggg agttgattga tacgtttacc atcatcaccc aacctgcaaa
     4441 tgagctgatg agccaaatcc acaacacaaa aaaacgaatg cctgcgattg ttgacaaaga
     4501 cctggtgaag agttggcttg atgctaagct accaaccttg aagtatgatg ccattcttag
     4561 accgctggac agcgaactta tggatgccca tgttgtgcct tcacccctaa gaaggcctaa
     4621 atcacagtaa tgcttaaaaa aacctgtcgc ttatgtttca tgtactttcg acaggttttt
     4681 ttgcataata cctgatattt cattagttga acaaggcagt gctgggtgca attcctacac
     4741 tataactttt tagggcagcg ccagcagagc tatatatttt cagctcaccc gccgaggtaa
     4801 aatttggtgc aaccatgcag tagatatcac cattgctggg cctaatgttg aacccataaa
     4861 agaaaccatt aataacaggt gtttggggaa tcacgggttg agtaatgctt acagagaaaa
     4921 ttccatttac cccgaatcct ccgccaaagt acacggtttc ttttcccgaa cttacatcga
     4981 tatgagcagg gtggctggta ttggaaataa tcaggctatg aactacttgc cggctctgac
     5041 ggtctatcat taccagcttg gaaggcaaat gacctacaac ttcccagctg tcatcgtaga
     5101 tatcggcacc ggaacaaata acccagatat tcccgctggc atcctcaacc aactctttgg
     5161 gattgtagcc aacagttacg gtgtccgaaa cagcgccggt tgcagcatca accaccataa
     5221 ccgtggagtc taagccatac cctcctccat tggcgaccca aattttccca tcggtacaaa
     5281 gtaaaccctc tgcgcctgtt ccaaccttga tagtattaga atgtgaaaag ctattcgcat
     5341 cgaacacctc gacagtgccc tgctcgcccc aactacccca ttgggtaaca taaatttttc
     5401 catcttttgc agccacataa cgtgggttta ccagtccagt aattgtttga agttgaatga
     5461 atgtagcgct gctggccacc tccaccttgc cagagttgtt caaaaccatg taaatctttc
     5521 cattatagat agcggcatgc tgaagcacat cgcccagctc acggccattt atgcggtaaa
     5581 atatttggtt atcggcaata tctgaaccgt aaccaatgaa ggtaatcgaa gcattggagt
     5641 taccgtaagc tccctcgttc agaataattg caccgttttc aaactcagca tactgagttt
     5701 catcatcacc cccgcaagag gcaaaggcaa aggcaagcac aagcatcgcc atcattaatt
     5761 ttactaattt cttcatggtt aaaggtttaa gttaatgaat caatttacct ggtaaagtca
     5821 atatttatgg tgaacatata actcctaagt ggcatggcgt atccggcaat tagctggtag
     5881 tcttgattcc acaagttatt caccgaaagt ccggctgtta caaccttcca aagtaggttg
     5941 aacttgtagc taataaagag atttgcaagg ttaaatgccg gcaactggct ttgaaaatcc
     6001 ctatagcgtt tgccggtaaa accgtgatag tatcccagcc catacttttc atactgtagg
     6061 ttaagagagc cggaaaacct gtgggttggt gtgtaaatca ttggttgagc cctactaccc
     6121 ggttgctcac tgaatacgga atgggtaaaa gtgtaaaatc cttccacacg aatagtaagg
     6181 ctgttaaccc ttaaactcca atcgacctga gactcccaac cccacgattc gcctttgctc
     6241 atgttgaaag gcttccattt actatcggaa accgtaggaa tgctgagcca tacaatccaa
     6301 cgctcaatat tgctgtagaa tagcgaattt gaaacgttta ttgccctatc gctgctgatg
     6361 ctctttacct gaaatcccac atcgccaccc caacccgatt caggttcaag attgggattt
     6421 ccctcggtat atgttgtgct ggcccagtag aggtcgttaa gtgtaggtat tcgataattt
     6481 ttggaggcat tcattttaaa gattaacttt tttgtgggaa gccattccgc tccaacagaa
     6541 taaatgacag gtttgaaatc accatcaacc aactctccac gaatgctcag cactgataaa
     6601 aatcttgagc taaacaaagg cttacgaagc gatgcatatc ctgaaactct gtttcttttt
     6661 gcacttccta cataatcgtc ggaactggct gtttcgaggg tgtggttaat ccccatgctt
     6721 acctccccat tattcagaac atccactttt gattccagct cagtgagaat agaggcgtag
     6781 ctgttgttgg taaaaatatt aacgccctta tccgagtagt ctataactcc caaacttgca
     6841 gcatttttca ggttgaaaag aatcctttta gaaggagaga agctaaggtt ggaaaccaac
     6901 gccaggcttc ggtcaacctg gttggcgtag ctgggctgcg tgctgctcat aatgggttgc
     6961 agatttttgt cgcctagctg gccccatccc gagaacgacc aaaataattt ttcatgcagc
     7021 cgcatgttga catcggtcaa aattcccagc tgtttcgact cagcatgtgt aatgcgttcc
     7081 tttgggctgc caattttgta aatattacga aactcgaagt cattatcagc taattgtcca
     7141 aaaaccttaa ccgaaatcgc ccggcttttg tcgccatact taacctgtga tgaaaggctg
     7201 cgggtatttc cgcttccgta tgaaagggat agctccccac cattaggctg aggaaataat
     7261 gcattgctgt tcaaaattag tattcccgat atggccccac tcccataaat ggttcctgac
     7321 ccgccatact gcaatctgat gctgctgaac atgtttacag ggaatagcga aaggttagca
     7381 cccccattca tcgggttttg aatgttcacc ccattccaaa ccacgatagt ttgtgaggag
     7441 tttccgcctc gcatggttag cgatgaaagg ccgccaacac cgtacgattt gaggttcacc
     7501 gaacccattg ccagcaactc ggcagctgat cgattttgaa agattttcag ctttacagaa
     7561 tccattctat aaatggtggc acctatagaa tggctttcgc gtaaattgcc gtaaaccttc
     7621 acttcgtcca tgcgaatggt gtcggataac gacatttgag cacacaaata accgaccagc
     7681 acaagctgaa gaaaaacgat cgagcaaacc cttttaagat gcatagctac aaatgattaa
     7741 taggcaatca atcattcgca gcactcggaa atagtgaaaa gtagaactga atggaacagc
     7801 gttctaatct tcgcctttca cccgaaagct acgaaaatac aacttggcag gtcttctggc
     7861 ttgctcggtc ttttaagcct tcccaaccgc aggttggcgg tcagtggcag gggtaaaaag
     7921 gccattacat tgagcttaca gctgcgggga cagctccggt tttacaccgg attccctttt
     7981 aatcccatac gggagccaat aattgttaca aatgtatgat tagattttta aaccgtcaag
     8041 taaatttagt atgcttaaaa acaccacccg ttgatttagg gtataatatt ttattaagct
     8101 actgctttta ccttaatttt tcaatccgct tcttcggagc agtggatcta tgcttggttc
     8161 gtgtcctcta aaacgcttgt agagctccat gggatgctcg ctcccacctt tagatagtat
     8221 gtttgttcta aagctagcag caacttctgg gttaaaaatt ccatgctcct tgaacatttc
     8281 aaaggcatcg gcatcgagaa cttctgccca tttgtagcca tagtaaccgg cagcataacc
     8341 gccatcaaat atgtgtccaa aacccacact ctggttggtt ccctcaacag taggtaaaac
     8401 ttcaaggttg gcaattgctc ttttttcaaa ctcaccaacg cttatcgtta cgggtttttc
     8461 aacggtgtgc caagccatat ccagcacgcc aaagctgagc tggcgaattg agctgtagcc
     8521 cgccaggaag ttacggctat caataatttt ctgaaccagc tcctggggta tcttttcgcc
     8581 ggtttggtag tggctggcaa acatgtcgag gaattccttt tcaaccgccc agttttccat
     8641 taattgtgat ggcagctcaa caaaatcacg gtaaacggat gttccgctaa gtgaggagta
     8701 agtgcattcc gaaagcattt cgtgcagggc atggccaaac tcgtgcatga atgttgaaac
     8761 ctcgtaaaat gtcagcagcg agggttgggt tgccgtgggt ttggtaaagt tggtaactag
     8821 cgaaacaaaa ggtctaacgt cagtgctccc atccctatac tgtgaacgga aagaggtcat
     8881 ccaggcgcca ccgctcttcc cttcacgggg gaaaaagtcg aggtaaagaa ccgccatgaa
     8941 cttatgcgtg gcatcataaa cctcgtaggc cttaacatca ggatggtaaa ccggtatttg
     9001 gttgttgggt gtaaaggtta agccgtaaag cttgttggca agtgtaaaaa caccgttaat
     9061 caccctattg agttcaaagt atggcttaag ttcctcctcg ttgtagctat agcgactatt
     9121 tttcaacttc tcggagtaaa acgaccaatc ccagggttga atattttcat taaaacccag
     9181 ctctttggca aaaccccgaa cctcttcaac ttcacgtctg gcagcaggca tagaagcatc
     9241 aaccaactcc ttcaggaagt tattcaccct ttcggggttt tcggccattc tgtcctgaag
     9301 aacaaacgaa gcatagttag gatagcccaa caggttggca atttctaacc gtaggttggc
     9361 aattttctta gcaatagcct ggttgtcgta ctcatttccc ttaaagcaac ggctaccata
     9421 agccatatag agttgtttgc gcagctccct attttcggaa tacttcatga atggaccata
     9481 cattgggtaa tcaagcgtaa ttatccatcc aactttcccc ttttgctgag ctgtgtaggc
     9541 agcggcatcc tttaaactag ccggaagtcc tgccaagtcg ctttcgttgg taatatgaag
     9601 ctggtagctg tttgtttcgg caagcaggtt ttcgttaaaa cggagcgata agtcggccaa
     9661 ctccttactt atggcacgaa acttctcctt gtcgctatcg gaaaggtttg ccccgtttct
     9721 gataaatccc ttgtaggtat tgctgagcag cgtttgctgt tcggtggtaa gcttcaggga
     9781 gtcacgcatt tcccacacct gcttaaccct tttaaaaagt tccgggttga ggttgacatc
     9841 gttgctaaac tcggtgagct tgatggccgc atcacgagca ataccctgca gggtcgaatc
     9901 cgtttcggac tcattgaggt tgaacagaat gttcgacacc aggttcaaac gcctaccgct
     9961 tcggtcgagc gcttcaatgg tgttttcgaa tgtacttttg gcaggattac tagtaatagc
    10021 ctcaacctca gcctttgcct cggcaatggc cgaatcaatt gccggggcat aatgctcatg
    10081 ccttatctta tcgaagggcg gagtttggaa tggtgtgtca aattcagcca gcagggggtt
    10141 tttcacctca ctgctattat ttttgcagga tgctgcaact agtataacta tcattgttcc
    10201 agtaagtttg ccgtagatat tcattttaaa aacgatttaa aagtgttggc cgcaaaggta
    10261 gaaaatt
//
LOCUS       DAOPHX010000095        10115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_8176_length_10115_cov_5.415109, whole genome shotgun sequence.
ACCESSION   DAOPHX010000095 DAOPHX010000000
VERSION     DAOPHX010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 10115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10115
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8176_length_10115_cov_5.415109"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="PK502_07750"
     CDS             <1..966
                     /locus_tag="PK502_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPH00748.1"
                     /translation="ERNMRSWLLTLVLLPYLFGTLAGQERQENLYVNEANQIFVKADA
                     PIYFFVSPSGQPQNMIMLPSSDSLANPMYFDGDGKHYLVYQSSGQKVRYLVYADGVGP
                     NPYVEVARGLLMKHQNRIYLEPNATFTPKAKDKRSGVKSVYYSLNGEPYKLATNELSP
                     GSNGEFSLHIYAVDNVGNTSDTAAYTLISSPDVTFRVNNIYFETASVNLLPESFEVLN
                     QMEDMLKLYPELHVEIKAHADTRGNTDYNLDLSERRAQSVVRYLSGKGISESRLKAVG
                     FGDSQPVNECLKGVQCPDSKHRENRRIEFRFFIPKLMQPTPKLSL"
     gene            968..2656
                     /locus_tag="PK502_07755"
     CDS             968..2656
                     /locus_tag="PK502_07755"
                     /inference="COORDINATES: protein motif:HMM:NF025125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPH00749.1"
                     /translation="MKGLLKYLFALTFAILLSHSIFAQNATRVLYMDTTGKMLYASPN
                     VEVNLYINTTNSASGAVKLTGLEGKGNPLRWSGHGLQVMTHLNLYIGRKIRFELFADG
                     KPPVSAVTSTNRNQKRIDEAVYIPGGAVIEVNAADEDSGLEDTYYSLNGEPFVPYTSP
                     LALDKDGVYEFTVYSTDKLGNREDEVTRKFVVDATPPTSAITVEGDQFEQVVSGRSVF
                     KLSATDEFGVKEILYRIDSSKFEAYRYPIKAGSLTDGDHVLEWYATDMVGNSEALQSH
                     HFTVDKTPPIVVEEIAGNTYMVGNVEYSSGRSQLRIVAIDNRAGIKDIFYSLNNGEYI
                     RYEKQVMLSDILGTINVKTYAVDNVNNRSQSGANTESFVMPTIDITGPTIGYQLLGRQ
                     LTLRDTLWIGQSTLVKLNAADKESGANRILYTIDKSGGMDYTGPFSLTEPGFHEIRCT
                     AYDNVDNLNFLSFKLGLDSEAPKIYHHFSVEPFKELPVGNVPVYPASVKLYLAATDNL
                     TGGVSVKYSVNEGKLTDYTQPIENFKPGNNYTLSITATDELGNSSTQTINFRVE"
     gene            3100..5013
                     /locus_tag="PK502_07760"
     CDS             3100..5013
                     /locus_tag="PK502_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HPH00750.1"
                     /translation="MNLHRYTIKKLLSFTSTIILLSFLLFTSIYYIRQQAVRQDKNIL
                     QRLDRLEQNSLRIKMLQDEFLLRDFHNSQLYVLGQSPATLRLDSLFTKTLEELNYLKK
                     YFSSTNITEVERHIEQYKKNFNNLKEMEITRGFKDFGLEGNLRNIIHQVEGNAVLNND
                     YRLLSGMLMLRRHEKDYMLRRDTLYVEKFDATLASTLNYAADKYGANNQFSKQLIDYS
                     KLFHEFLSIDAQIGRTENEGATYQLQQQSALVIESIRNLYGELSENIKKKDREAYASM
                     LMLLVIVSSLVLTLLTKISRHITKTIKSTLAVIKKLGKGELPPPLEVKGADEFSAMEK
                     SINDLSLALRNTRDFAIEVGNGNFYSEVNVFGNSGELGSGLLEMRQKLTEVAVQQENS
                     LKEKEQRSWLNECLAQVSELLRNFHSDTKELCFEFVRFAIKSTGSLQGGIYLLNSEGE
                     SDSDENSYLELVASYAYDRRKYINKRFEKYEGVVGSCLYEKDVIYITDIPDSYTHITT
                     GLGVSNPSCIVLTPLVTEKEEALGVMEISSYSPITDIQVEFMKQACRNLASSLVLHKA
                     IKANEQIIAQMKVKTEELLANEEELRQNMEELTAIREDMERREKDLNGQIAELKYKLR
                     LHGIIFEADTYHN"
     gene            complement(5080..5751)
                     /gene="ung"
                     /locus_tag="PK502_07765"
     CDS             complement(5080..5751)
                     /gene="ung"
                     /locus_tag="PK502_07765"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPH00751.1"
                     /translation="MNPQIEESWKKVLAGEFQQEYFRELKNFLVEERRQYRVYPPGSK
                     IFAAFDFTPFTSVKVVLLGQDPYHGHGQAQGLCFSVPRGIPKPPSLVNIFKELHDDLG
                     LAIPPHGNLEAWARQGVLLLNATLTVRANQAGSHQHKGWEQFTDAAIRALAEQRENLV
                     FMLWGNYAQAKAALVDGHKHCILKAPHPSPFSANQGFFGCRHFSRANDFLVRAGKEPV
                     DWQIS"
     gene            6082..6651
                     /locus_tag="PK502_07770"
     CDS             6082..6651
                     /locus_tag="PK502_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPH00752.1"
                     /translation="MNADQQTSEQFYWLVRNRQSDRAYSNRPVDREILVRVVEAARLA
                     PSACNAQPWKFIVVDDPELKNRLADAAASKILGINHFTKQAPVHIVIVLEGANLNSNF
                     GSMVKGKTLPLIDIGIAAEHICLAAKSEGLGSCILGWFDEKAVKQLLDIPKSKRVPLI
                     ITLGYPKTEDIREKRRKPLEKIISFNGYS"
     gene            complement(6656..7756)
                     /locus_tag="PK502_07775"
     CDS             complement(6656..7756)
                     /locus_tag="PK502_07775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPH00753.1"
                     /translation="MKAIKVSFCTTSNLITDQRIQRAASTLSRNGYQVNVFALRRHGL
                     PNEKPFEFAVKRFRCVFSDGPMFYVEYNLKILTRLIFTRTSLIYSNDLDTLPGCWLAS
                     ILKAKPLVYDSHELFTEIPELIGHPVKKRFWAIAERVCIRRAKCVITVSEGVAKELST
                     RYGVKPVVVRNFSVKRDKSPYRDLKPTLIYQGSLNVGRGIELAIDMMEWLPCYHLLVI
                     GSGDIEIPLRRRAIERGLTDRVEFMGRVDPDRLPDVTGRAWLGLSLEEDLGLNYRLAL
                     PNKIFDYIASGVPILVSDLPEMRKIVETYHVGLVANTRDPEKLAGIVADFVENKKTRE
                     VMVNNVLAASETLCWDNEYPVLLNAVGKAIGC"
     gene            complement(7873..>10115)
                     /locus_tag="PK502_07780"
     CDS             complement(7873..>10115)
                     /locus_tag="PK502_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPH00754.1"
                     /translation="LTMEIYYLIILILLFVLAISDLMVGVANDAVNFLNSAIGSRVAA
                     RHWILLVASVGVLVGAVFSSGMMEIAQKGVFRPEKFYMSEIMTLFMAVMLTDVIVLDF
                     FNTFGLPTSTTVSLVFELLGSAVAISIFKISENNESIADLGSYINTAKALAMISGIFI
                     SVVIAFFIGTIVQYIARVLFTFNYTRSMKYVGSIWGGISLTAITYFIAMKGLNSTALV
                     SPDTLNFINQHTMVILLLSFTGWTIIFQLAISIFKWNILRFVVLAGTFALALSFAGND
                     LVNFIGPTMAALKTYQVHLANPGVAPDQLLMTDLAGPVSTNTLYLLIAGLVMVITLWT
                     SRKARTVTNTEVGLANQDSGAERFGSTQLSRSIVRISRSFSEGVGRITPLRIKSFLSR
                     RFDLSKALVVKNPKDAPAFDLVRASVNLVVSSILISLGTSLKLPLSTTYVTFMVAMGT
                     SLSDRAWGRESAVYRVTGVITVIAGWFFTALITFIVSFVVAAALFYWKAFALVGLAIL
                     AGWLLIRSNLIHRSRNQNRDVGAELITDKSVVEKCTLGVNNSLRDVVEVFSQTIDGLT
                     SENRKLLRTVNNRVADLNSNSKNFKNNIYQTLKKLEADSVETGHYYVQVVDYLREIAH
                     SLSFITEPSLKHIENQHKGLTTDQAIELTTISNQVSDFFQEIMKIIKNNDFTGVPQVV
                     SKQQDILDKLNEARKNQVKRIKQGVTGTRNSVLYLGLINEIKNIMLHTVNLLKAQRDF
                     ILNNVE"
BASE COUNT         2973 a         2446 c         2247 g         2449 t
ORIGIN      
        1 gagcgcaata tgagaagttg gttgcttacc cttgttctgc tgccatattt gtttggaact
       61 ctagccggcc aggagcggca ggaaaatctt tacgtcaacg aagcgaatca aatattcgtt
      121 aaagccgatg cccccattta cttttttgtt tctccttctg gtcaacccca aaacatgatt
      181 atgctgccaa gcagcgatag cctggctaac ccaatgtact tcgatggtga cggaaagcac
      241 tacctggttt accagagtag cggccaaaaa gttcgctacc tggtgtatgc tgatggagtt
      301 ggtcccaacc cctacgtcga ggttgcccgg ggcctgctta tgaagcacca gaaccgcata
      361 taccttgaac ccaacgcaac cttcactcct aaggcaaagg ataaacgttc gggggtaaaa
      421 tctgtttact actccttaaa cggcgaaccc tacaagctgg ccaccaacga gttgtcgccg
      481 ggtagcaacg gagaattcag ccttcacatt tatgctgttg ataatgttgg taatacaagc
      541 gataccgcag catacaccct aatttcttct cccgatgtta cattcagggt aaacaacatt
      601 tactttgaaa ctgccagcgt aaacctgcta cccgaatcat tcgaggtttt aaatcaaatg
      661 gaagacatgc tgaagctcta ccccgaactc catgttgaaa taaaagcaca tgccgacacg
      721 cgaggaaaca ctgattataa ccttgacttg tccgaacgaa gggcacaatc ggttgttcgt
      781 tacctttcgg gcaagggcat cagcgagagc aggctgaagg ccgttgggtt tggcgattcg
      841 caaccggtaa acgaatgcct gaaaggggtg cagtgtcctg atagcaagca ccgggagaac
      901 cggagaattg aatttcgctt ctttattcca aaactcatgc agcccacacc caaactcagc
      961 ttataaaatg aaaggactgc taaaatacct attcgcccta acctttgcca ttcttttatc
     1021 gcatagcatt tttgcccaga atgccacacg tgtcctgtac atggatacaa ccggaaaaat
     1081 gctctacgca tcacccaacg tggaggtaaa tctctacatc aacacaacca actcagcctc
     1141 aggtgcagta aaactcacag gtctggaagg aaaagggaat cctttgcgct ggagcggaca
     1201 tgggctccag gtgatgactc accttaacct ctacattggc cgaaaaattc gttttgagct
     1261 gtttgccgat ggtaaaccgc ctgtatcagc ggtaacctcc accaaccgga accagaaacg
     1321 tattgacgag gctgtgtaca tacctggcgg agcggtaatt gaggtgaatg ccgccgatga
     1381 ggattcaggc cttgaggata cctactactc tctcaacggc gaaccctttg ttccctacac
     1441 ctctcccctg gcactcgaca aggatggagt ttacgagttt accgtgtact caaccgacaa
     1501 gctgggcaac cgggaggatg aggtaacccg taaattcgtg gttgatgcta cacctcctac
     1561 atcggctatt accgttgaag gtgatcagtt tgaacaagta gtatcgggac ggtcggtttt
     1621 taagctgagc gccaccgatg agttcggtgt gaaagaaatc ctctaccgga ttgactcgtc
     1681 aaaatttgaa gcctaccggt atccgatcaa ggcaggaagt ctgactgacg gagaccacgt
     1741 tttggaatgg tacgccaccg acatggtggg taatagcgaa gccctgcaga gccaccattt
     1801 cacagtggac aaaacaccac caattgtagt ggaggaaatt gccggcaaca cctacatggt
     1861 gggcaatgtg gagtactcat cgggtcgaag ccagcttaga attgttgcca ttgacaacag
     1921 agccggcatt aaggatattt tttactccct taacaatggc gagtacattc gctatgaaaa
     1981 acaggtaatg ctctcggata tacttggaac cataaatgtg aaaacctacg cggttgacaa
     2041 tgtaaacaac cgcagccagt caggagcaaa caccgaatcg ttcgtaatgc ccaccattga
     2101 cataaccggc ccaaccattg gttaccagct gctgggccgc cagctaaccc tccgcgacac
     2161 cctttggatt ggacaatcaa cgctggtaaa gctaaatgct gccgacaagg agtcaggagc
     2221 aaaccgcatc ttatacacca ttgacaaatc gggtggcatg gactacaccg gcccatttag
     2281 cctgaccgaa cccggattcc acgaaattcg atgcaccgcc tatgacaacg tggataatct
     2341 taacttccta tcgttcaaac tagggcttga tagcgaagct cccaaaatct accatcactt
     2401 tagcgttgaa ccctttaaag agctacctgt tggtaatgtt cccgtttacc ccgcaagcgt
     2461 aaagctttac ctggctgcaa ccgataacct gacgggaggt gtttccgtaa agtattcggt
     2521 taatgagggc aaactaaccg actacaccca accgattgag aacttcaaac ccgggaataa
     2581 ctatacctta tccatcacag ccaccgatga gctgggaaac agctcaacac aaaccatcaa
     2641 ctttagggtg gagtaggttc caaccggaga gacttgcccg aaaacacagc atgaatagca
     2701 acaccgggga gaatttcatt ttcctcgacc aaagcccttc cttcggattg ccataaacat
     2761 ggccggattt cccttctggg agtcccctct ctaacgcaat tgctaccaac cagccgcttc
     2821 tggaaccttg ttcaatataa gaaaatcgct actgacaggt aagcatatag cttactgata
     2881 aaaaacagca gaaaaaatat caccacttat gaaatttaaa attgcatagc tataccctta
     2941 tttaaccagg caaaaaattg cgtagcagtg ctccagctac tgctttacaa aaggtgtaac
     3001 aataacaaca ttaaccattt aaacaattaa cacataatta attgaatgga aaccttgaat
     3061 atataaataa ttggaaaatt tgaagaaaaa taagtagcca tgaatctaca caggtacacc
     3121 ataaaaaaac tgctctcctt cacctcaacc atcatcctcc tttcatttct tcttttcacc
     3181 tctatttact acattcgaca gcaagcagtg cggcaagata aaaacattct tcaaaggcta
     3241 gatcggctag aacaaaacag cctgagaata aaaatgctgc aggatgaatt tttgctacgc
     3301 gacttccaca acagccagct ttacgtgcta ggacaaagcc cggcaacgct gaggcttgac
     3361 agcctgttta caaaaaccct cgaagaactc aactacctaa aaaaatattt tagttcgaca
     3421 aatattaccg aggttgagcg gcacattgag cagtacaaaa agaacttcaa caacctgaaa
     3481 gagatggaga ttaccagggg atttaaggat tttggacttg aaggaaacct cagaaacatc
     3541 atccaccagg ttgaagggaa tgccgtactg aataatgact acaggcttct tagcgggatg
     3601 ctcatgctca ggcgtcacga aaaggactac atgttgaggc gtgataccct gtatgttgag
     3661 aaattcgacg caacgctagc gagcacgctt aactatgctg ctgataagta tggggcaaac
     3721 aaccagtttt cgaagcaact gatagattac agcaaacttt ttcatgagtt tctatccata
     3781 gatgcacaaa taggacgcac agaaaatgaa ggagccacct accagctaca gcaacaatcg
     3841 gcactggtta ttgaaagcat tagaaatctt tatggcgaac tttcagaaaa tatcaaaaag
     3901 aaagacagag aggcttatgc ctccatgctc atgttgctgg ttatcgtgtc gtcgttggtt
     3961 cttacgctgt taacaaaaat aagcaggcat atcaccaaaa ccatcaaatc aacgctggct
     4021 gtaattaaaa aattggggaa gggagaactt cccccaccac ttgaagtaaa gggtgccgac
     4081 gagttttccg caatggagaa atccatcaac gacttgagcc tggctctacg caacacccgt
     4141 gattttgcaa ttgaagtagg gaatggaaac ttctacagcg aggtaaatgt tttcgggaac
     4201 tccggtgagt taggatcagg cctgctcgaa atgaggcaga agctaaccga agtggctgtt
     4261 cagcaggaaa acagcttaaa ggagaaggaa cagcgaagct ggctcaacga gtgcctggcg
     4321 caggtttcag aactactaag aaatttccat tccgacacca aggagctttg ctttgagttt
     4381 gtccgctttg ccattaaaag cacaggttcg cttcagggtg gcatatacct actcaatagc
     4441 gaaggtgaga gtgatagtga tgaaaactcg tacctggaac tagtggcctc ctacgcctac
     4501 gaccgacgca agtatataaa caagcgattt gaaaaatatg agggagtagt ggggtcttgc
     4561 ctctacgaaa aggatgtaat atacatcacc gatatacctg atagctacac gcacatcacc
     4621 accggtttag gcgtttcgaa ccccagctgc attgttctta ccccactggt aaccgaaaag
     4681 gaagaggcgt tgggggttat ggagatttcc agctacagcc ccataaccga catacaggtt
     4741 gaatttatga aacaagcatg ccggaatctc gcctcctcgt tggttcttca taaggcaatt
     4801 aaggccaatg aacagattat agcccaaatg aaggtaaaaa ctgaggagct actggccaac
     4861 gaggaagagc taaggcagaa catggaggag ctgaccgcaa tcagggagga catggaacga
     4921 cgggaaaaag acctaaacgg acaaattgcc gagcttaaat acaagctaag gcttcatggg
     4981 attatttttg aagccgatac ctatcacaat taggctgcac acgccagtgg gtggaggagt
     5041 atagactttt caactgccca gcgctaaaag taaatgcaac tagctaattt gccagtcaac
     5101 tggttccttc cccgccctaa ccaaaaaatc atttgccctg gagaagtgcc tgcaaccgaa
     5161 aaaaccctgg ttggctgaga agggcgatgg gtgtggggct ttcagtatgc aatgcttatg
     5221 cccatcaact aatgccgctt tggcctgtgc gtagtttccc catagcataa aaaccaggtt
     5281 ttcgcgttgc tcggccaatg ctcttattgc ggcatcggta aactgctccc aacccttatg
     5341 ctggtgcgac ccggcctggt ttgccctcac agtaagggta gcattgagca aaagaacacc
     5401 ttgccttgcc catgcctcca ggttaccatg aggaggaatg gctaaaccca aatcgtcatg
     5461 cagctcctta aatatgttca ccagcgatgg aggcttaggt atacctctgg ggacagaaaa
     5521 acagagtcct tgcgcctgcc catgcccatg gtagggatcc tgacccagca ggacaacttt
     5581 cacgctggtg aatggggtaa agtcaaatgc agcaaagatt ttagaaccgg gagggtaaac
     5641 cctgtactgc ctccgctcct caacaagaaa attctttaac tccctgaagt actcctgctg
     5701 aaactccccg gccaaaacct tcttccagct ctcctctatc tgcggattta tgctaacatc
     5761 gtccatggct acctacctga aaaacggaac aagtgaatta tcgaacccct aaaattactt
     5821 tcaatttgta ttcttacaaa gaattctcct catctttcaa tattaagcct gcttgcgatt
     5881 tcgtagcttt gccatgttca tattacacat caagttcaac gttggcttgg cagccaatct
     5941 actttttact atcttcgacg tccgaattag aatgggcagc ttgaaggttt ttgaaatttc
     6001 aacctgcata aatttccacc ttaatgaagc ttatagcttg taaaagacaa tgtttttaat
     6061 cctttctaaa caactcctac catgaatgcc gaccaacaaa ctagcgaaca attctactgg
     6121 cttgttcgaa acaggcagag cgacagggct tacagcaacc gaccggttga tagggaaata
     6181 ctggtgaggg tagtggaagc agcccgtctg gctccatcgg cttgcaatgc tcaaccgtgg
     6241 aagtttattg tggttgatga tcctgagcta aaaaacaggc tggccgatgc agcagccagc
     6301 aaaatactgg gtattaacca ttttaccaag caagcgcccg ttcacattgt tattgtgctg
     6361 gagggagcta acctgaactc caactttgga agcatggtaa aggggaaaac actaccgctt
     6421 attgacattg gcattgctgc cgaacatatt tgcctggcag ctaaatcgga aggtttgggg
     6481 agctgcatac tgggttggtt cgacgaaaaa gccgttaagc agctactgga tatacccaag
     6541 tcaaaacgcg ttccgctcat catcacgcta ggctatccaa aaaccgagga cataagggag
     6601 aagcggagaa agccgttgga aaaaattata tcatttaacg gctacagcta actgtttaac
     6661 agccaatcgc ttttccaaca gcattcagga gaaccggata ctcgttgtcc cagcatagtg
     6721 tttcagaggc cgctaataca ttattaacca tgacctccct ggtcttcttg ttttcaacaa
     6781 agtcggctac aatgcccgcc aacttctccg gatctcttgt gttggccact agtcctacat
     6841 gataggtctc aacaattttt cgcatctcgg gtaaatcgct aaccaggatt ggcacacctg
     6901 aggctatgta gtcgaaaatc ttatttggca gagcaaggcg gtagttcaac cccaaatcct
     6961 cctcaagcga taagccaagc caggccctac cggttacatc gggtagcctg tccggatcaa
     7021 ctctgcccat aaactcaacc ctgtcggtta gcccccgctc aatagctctc cgcctaagag
     7081 gtatttctat atcgccactt ccaataacca gtaagtggta gcaggggagc cattccatca
     7141 tgtcgatggc gagctctatg cctctcccca cattcaacga tccttggtat atcaacgttg
     7201 gcttcaaatc cctataagga gacttatcac gcttaaccga aaagttacga acaacaacgg
     7261 gcttaactcc atacctggtg ctcagctcct tggcaacacc ctctgaaact gtaatgacgc
     7321 attttgccct tcgaatacat acccgctcgg caatggccca gaaacgtttc ttaacaggat
     7381 gccctattag ctctggtatc tcggtaaaaa gctcatggct atcgtaaacc aaaggtttgg
     7441 cttttaaaat tgaagccagc cagcaccctg gaagtgtatc caaatcgttg gagtatatta
     7501 agctagtgcg ggtaaaaata aggcgggtta agattttaag attgtactcg acataaaaca
     7561 tgggaccatc cgaaaacaca caacgaaaac gctttactgc aaattcaaaa ggtttttcat
     7621 tcggtagtcc atgcctcctg agggcaaaaa cgttaacctg atacccattc ctggaaagcg
     7681 tgctagctgc tcgttgaatc cgctggtcgg ttatcagatt tgaggtagta caaaaactta
     7741 ctttaatggc tttcatccgg ataaatgtat gctctatagt ccataattcc aaatcataca
     7801 ccttttagca agctggcaaa ccccataatg tttttcagca agaaagctaa atgggaccat
     7861 gctggaagac cactactcaa cattattcag aatgaaatcg cgttgagcct tgagcaggtt
     7921 aacggtgtgc agcataatat tcttaatttc gttgatgagc ccgaggtaca gaacgctgtt
     7981 tcgggtaccg gttacaccct gcttaattcg cttaacctga ttttttcttg cctcgttaag
     8041 cttgtcaaga atgtcctgct gcttgcttac aacctgtgga actccggtaa agtcgttatt
     8101 ctttattatt ttcattatct cctgaaagaa atccgaaacc tggttgctga tggttgttaa
     8161 ctcaatggcc tgatcggtag ttagcccctt gtgctggttc tcaatgtgct taaggctggg
     8221 ctcggttata aacgagagtg aatgggcaat ctccctgagg taatcaacca cctgaacgta
     8281 gtagtggcct gtttcgacag aatcggcttc cagcttcttt agtgtctgat agatgttgtt
     8341 tttaaaattt ttggaattag aattaaggtc agctacgcgg ttgttgaccg ttctaagcag
     8401 cttcctgttt tcggaggtta acccgtcaat ggtttgggaa aacacctcaa ccacatctct
     8461 tagggagttg ttaaccccca atgtgcattt ttcaacaaca ctcttatcgg taattagctc
     8521 tgcaccaaca tccctattct gatttctact gcggtgaatt aagttcgacc tgatgagcag
     8581 ccaaccggca agaatggcta accctaccag ggcaaaagct ttccagtaga aaagagcagc
     8641 agcaacaaca aacgatacga taaaggttat gagagctgta aagaaccaac ctgcaatcac
     8701 ggtaataaca ccggtaaccc ggtaaacagc gctttccctt ccccacgccc tatcgctgag
     8761 cgaggttccc atggcaacca taaaggtaac ataggtggta gaaagcggta gttttaggga
     8821 agttcccaag ctgatgagaa tgctggaaac caccaggtta acagaagcac gtaccaaatc
     8881 gaaagcagga gcatccttgg ggttttttac cactaaagcc ttacttaaat cgaatctccg
     8941 ggaaagaaat gatttaatgc gtagtggagt gattctccca acaccttcag aaaaagaacg
     9001 ggatatacgc acaatagacc tggagagctg ggtgctgcca aaacgttcag ccccgctatc
     9061 ctggttggcc agtccaactt cggtattggt aacggttcgt gccttacggg atgtccatag
     9121 ggtaatcacc ataaccaatc ctgcaattag taggtaaagc gtatttgttg agaccggacc
     9181 ggccaaatcg gtcatcagca gctggtccgg tgccaccccg gggtttgcca ggtgtacctg
     9241 gtaggttttg agcgccgcca tggtggggcc aataaagttt accaggtcgt taccggcaaa
     9301 cgaaagggct agggcaaaag taccggccag cacaacaaac cttaggatat tccatttgaa
     9361 gatggatatg gccagctgaa agatgatggt ccacccggta aagctcagga ggagaatcac
     9421 catggtgtgc tggttaataa agttcagggt atccggcgaa accagggcgg tgctgttgag
     9481 acccttcatg gcaataaagt aggtaatggc cgtaagggag attcctcccc aaatactgcc
     9541 aacgtacttc atgctacggg tgtagttgaa ggtgaaaagc acccgggcaa tgtactgcac
     9601 aattgtgccg ataaaaaatg ctattaccac cgatataaaa atccccgata tcatagccag
     9661 cgccttggcc gtgttgatgt agctacctaa atcggcaata ctttcgttgt tttctgaaat
     9721 cttaaaaatt gaaatggcaa ctgcggagcc aagcagctcg aaaaccagtg ataccgtagt
     9781 ggaggtaggc agaccgaaag tattaaaaaa atctagcaca ataacgtcag taagcatcac
     9841 agccatgaac aaagtcatga tctcgctcat gtaaaacttt tcgggacgga acaccccctt
     9901 ctgggcaatc tccatcattc cgcttgaaaa aaccgcgcca accagcaccc caaccgaagc
     9961 caccagcaga atccaatggc gtgcggcaac acgcgatccg atagccgaat tcagaaagtt
    10021 taccgcatca ttggctacgc caaccattaa gtcggatatt gccagaacga acagtaaaat
    10081 aaggattata aggtagtata tctccatggt taggg
//
LOCUS       DAOPHX010000096        10081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_8218_length_10081_cov_6.953621, whole genome shotgun sequence.
ACCESSION   DAOPHX010000096 DAOPHX010000000
VERSION     DAOPHX010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 10081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10081
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8218_length_10081_cov_6.953621"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            492..959
                     /locus_tag="PK502_07785"
     CDS             492..959
                     /locus_tag="PK502_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPH00755.1"
                     /translation="MSSFIGDYECKVDEKGRILFPASLRKQVKSALPDQFVVKKDIYE
                     DCLTLYTMEEWQRQNEIIRTNTNPYNREHNAFLREFFRGTAEITLDSSGRLLLPKRLL
                     DMIGVVRDVVLAGMDGKVEVWAKEKYEGIRLESVQFASLAEKILGSTTIRDEK"
     gene            956..1855
                     /gene="rsmH"
                     /locus_tag="PK502_07790"
     CDS             956..1855
                     /gene="rsmH"
                     /locus_tag="PK502_07790"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPH00756.1"
                     /translation="MTYHVPVLLAESIEGLNIRPDGTYVDLTYGGGGHSRHILERLGK
                     KGKLYAFDQDEDALTNASDDKRFTLIRGNFKYFRNFLRYYNVAKVDGILADLGVSSFH
                     LDTPERGFSFRFEGPLDMRMNKRASLTAKKVVNSYSIEELSLIFNQLGELPDGRRYAQ
                     AIYDGRKVKQLETIEELTQILKPLVPAKLQNKVLAKIFQALRIEVNHELDALKLMLVG
                     SAKCLGREGRLVVISYHSLEDRLVKNFLKFGNFEGEDRPDLMGNRVVPFEQVNRKVIV
                     PSFDEISANNRARSAKLRIGRRL"
     gene            1852..2223
                     /locus_tag="PK502_07795"
     CDS             1852..2223
                     /locus_tag="PK502_07795"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HPH00757.1"
                     /translation="MSVSGETEEFVDIQPEKPREKKGFRFKDFLSGRILTHENVSSQL
                     PYILFLVLLAVIYIGNSYRNNQLLRNEQKIKIEVKNLRAESITTAAKYMSVSRQSEVV
                     KLVESKGLGLEESTIPPKKID"
     gene            2243..4366
                     /locus_tag="PK502_07800"
     CDS             2243..4366
                     /locus_tag="PK502_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HPH00758.1"
                     /translation="MSIKKDIVTRITVVYILFALLGVGIFVKIIYIQFVEGSKLREKA
                     KAITYRDIIVEPNRGDILASDGRVLATSVPYYDIRMDLAASGLSDQVFFSNIDSLAIC
                     LSRFFGDRSKYAYRSELVNARKYAHNKRFYPIAPRRVNYMELQTIKTFPLFRLGENKG
                     GFIAVQANRRILPHSKMAARTIGGVNQTGVAVGIEGAFDYALKGKAGLKVHERIPGNS
                     WVEVKSMNQIDPEDGCDVVTTIDVALQDVAEAALRKHLGNHDADHGCAVLMEVSTGDI
                     KAIANLKRNEDGTYSEVYNYAIGESTEPGSTIKIATLINLLEDGFVELNDTVNTGNGK
                     LRMYDRIITDSKEGGHGKISVKEVFEVSSNVGVAKLVTKYYRGKESHYVDRLYSMKLN
                     QPLGIGIPGEASPQIKYPTDRYWSGMSLPMMSIGYELKLTPLQILTFYNAIANDGRMV
                     KPRLVKSLERHGMVVQTFSPEVINPSICSRSTLRKVNEVLEGVVESGTAQNLKSTPYK
                     IAGKTGTAQLAKGRRGYKIGGSVSYQASFVGYFPSDKPRYSCIVVVNAPSQNIYYGNL
                     VAGPIFKEISDKVYATTPDWFREIDNGGSLKELPESKSTRLVMLKEILKQLDVPYQIA
                     NSKAEWVSTKRDSNEIALNTLSFSSKAVPSVVGMGLRDAIFLLENTGLHVSFNGRGTV
                     RSQSIAPGTAIKRGSNINLELSMSN"
     gene            4379..5845
                     /locus_tag="PK502_07805"
     CDS             4379..5845
                     /locus_tag="PK502_07805"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPH00759.1"
                     /translation="MAVGLTKILPKALVNEVVGNPDIEVEQLTFNSRVAGANSCFFAI
                     RGTQADGHRFIPQAVENGASAIVCEAMPVSFAPNVTYVRVSDSSEALGLMASAFYGNP
                     SSNLKLVGVTGTNGKTTTATLLYRIVGMLGFKAGLLSTVVNQVVDEKIPTTHTTPDPI
                     ELNRLLRRMVDAGCSYCFMEVSSHAVVQKRIAGLTFAGGIFTNITHDHLDYHKTFDEY
                     IRAKKMFFDQLPSESFALVNADDRNGMVMVQNTKAKVSTYALHSIADFRCRVVESHFD
                     GMLLNMDGLEVWTRLIGEFNAYNLLAIYASLLLLGFDKTDVLLQLSMVNSVSGRFEYV
                     SSSGGITAVVDYAHTPDALVNVIETIKKIKSSGQRLITVVGAGGNRDKTKRPVMARVA
                     VQNSDLVILTSDNPRFEEPEDIINDMKAGVDEEFTKKLVTIVDRHEAIRTACLLANKE
                     DIILVAGKGHENYQEVKGVKHHFDDKEVLAEIFNELHI"
     gene            5842..7086
                     /gene="mraY"
                     /locus_tag="PK502_07810"
     CDS             5842..7086
                     /gene="mraY"
                     /locus_tag="PK502_07810"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPH00760.1"
                     /translation="MIYYLVQYIQKQFDFPGAGLFRYISFRSALAFILSLLFALFIGK
                     RIIRYLQRKQIGEEIRNLGLEGQLQKKGTPTMGGIIVIAATLISVLLFADLLNIYIII
                     MLVTTVWMGAVGFTDDYIKVFLKNKDGLSGKFKILGQVLLGLFVGLTMWLSPDIMIRD
                     HVRPNIQSEVVVTSEYSGSEPVSVSKGKKTTQTTIPFFKNNEFDYAYLNPFKGKWRDA
                     ITWTIFIVVVIFIITAVSNGTNLTDGLDGLATGVSAMNATTLGVFAYLSGNVIYSEYL
                     NIMYIPHISELVVFMSALIGALIGFLWYNSYPAQVFMGDTGSLTIGGIIAVYAIVVRK
                     ELLLPILCGIFLVEALSVLIQVSYFKVTRKIYGEGRRVFLMSPLHHHYQKKGYAEPKI
                     VTRFWIISMLLAVITVVTLKIR"
     gene            7234..8568
                     /gene="murD"
                     /locus_tag="PK502_07815"
     CDS             7234..8568
                     /gene="murD"
                     /locus_tag="PK502_07815"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPH00761.1"
                     /translation="MKRIAILGGGESGAGAAVLAFKKGFDVFLSDSSTIKPRYREWLQ
                     RYSIPFEENGHTEALILNADEVIKSPGIPDKASIVARIREQGIPVISEIEFAGRYNTA
                     RTICITGSNGKTTTTSLIYHMMQKAGLNVGLAGNIGNSFAYQVAEDNFDYYVIELSSF
                     QLDGVYEFKPDIAILLNITPDHLDRYDYKMQNYVESKFRITRNLSEDDCFIFCSDDAV
                     TVEQLKRIILRAQQLPFSQRVKENQAAWLDDDNLLINYNNSDFSMSINDLSLKGKHNI
                     YNSMAAGIAGHVLNIRKDVIRQSLSDFQGVEHRLEPVLTIKGVRYINDSKATNVNSAW
                     YALESMDTPVVWIAGGVDKGNDYSELMDLVKLKVKAIVCLGVDNVKLHKAFGNVVPVM
                     VDVLSAEDAVRSAYELANPGDTVLLSPACASFDLFENYEDRGRKFKDAVRML"
     gene            8580..9749
                     /locus_tag="PK502_07820"
     CDS             8580..9749
                     /locus_tag="PK502_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPH00762.1"
                     /translation="MGTFVDKYFKGDRAIWLVVILLSAFSLLVVYSSTGSLAFRYHGG
                     NTTYFLVKQLVFLVLALSIVFVTHLISYRVYYPLSSLLMLISIPLLLFTLVFGTRLNE
                     ASRWLEIPGLGITFQSSDVAKFALVMYMAKILSQKQKDIKDLKQGFLPLALPVILICG
                     LILPANFSTAALLGLTCWIMMYVGRVNLKYLLGFTGIGVALFAIFVVVALHFGDVGRV
                     HTWKNRIENFVSGDNPDGNYQVEQSKIAIANGGITGKGPGRSTQRNFLPHPYSDFIYA
                     IIIEEYGLFAGLFIISLYLILLYRSAVIVKKAKRTFPAFLAFGLALLLTMQAMVNMAV
                     AVNLIPVTGQPLPMISMGGTSLFFSSAAFGIILSVSRLQSKKELFDGEEPAESNN"
     gene            9718..>10081
                     /locus_tag="PK502_07825"
     CDS             9718..>10081
                     /locus_tag="PK502_07825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPH00763.1"
                     /translation="MEKNQLKVIISGGGTGGHIFPAIAIANAVKELSPEAEILFVGAL
                     GRMEMERVPQAGYRIVGLPIIGIRRSLSPQNIMLVFKLIRSLRLSRKIIADFRPDVVV
                     GVGGYASGPILRAAQKRGI"
BASE COUNT         2656 a         2229 c         2538 g         2658 t
ORIGIN      
        1 tcccgcgcta cgttccgccc ccgacccatt tgcgggtcag gcttttatgc catttttcta
       61 atacctgcaa cgttcaacga ttcccgcctt tccgagcacc gctgagaata tctttccccg
      121 gtagggctga agaaaagggt aaagggtaat acaaggataa aggataaaaa aaattcaagc
      181 aggtttcaag aacttgtccg cctgctggcg gattcaccct tcgacaggct cagggttcag
      241 acttgtccac ctactggcgg attccagaac ttgtccgcct actggcggaa cttgtccggc
      301 tcctgacgga ttccgtaaac tgtccatcag ctaatgggtt tatccttaaa ggttgacggc
      361 taacatctca ccgattggcc taggtacgtc gctttcaaaa aaaagttatc aacaaaatgc
      421 aaaaaagtgg gtaaaagtgg ataaaagtgg taaagtattg tatattttta ccatttaaat
      481 gccagctgga aatgtcctca ttcatcggag actacgagtg taaggttgac gaaaagggta
      541 gaatactctt tcctgcatcg ctacgcaagc aggtcaaatc tgcgctgccc gaccagtttg
      601 ttgtgaagaa ggatatttac gaagattgcc ttacgctcta caccatggag gagtggcagc
      661 ggcaaaacga aataataagg acgaacacca acccttacaa ccgggagcac aacgcttttc
      721 ttcgggagtt tttcagggga acagctgaaa tcacgctcga ttcaagtggt aggctattgc
      781 taccaaagcg actgctcgat atgattggag tggtacgcga cgtcgtgctg gcgggtatgg
      841 acggaaaggt ggaggtttgg gcaaaggaaa agtacgaagg catacggctc gagtctgttc
      901 agtttgccag tctggccgaa aaaattttag gttcaacaac cataagggat gagaaatgac
      961 ctaccatgta ccggttctgc ttgccgaaag catagagggt cttaacatca gacccgatgg
     1021 cacgtacgtt gacctcactt atggcggggg tggccattcc cgccacatac ttgagcgcct
     1081 tgggaaaaaa ggaaagcttt acgctttcga tcaggatgag gatgccctta caaacgcttc
     1141 agatgataaa cggtttaccc taatcagggg aaacttcaag tactttagga actttctacg
     1201 gtactataat gtcgctaaag ttgacggtat tctggccgat ttgggagttt catcgtttca
     1261 cctggatact cccgaacgcg gattttcgtt ccgattcgag ggaccattgg atatgcgcat
     1321 gaacaagcgg gcctcactta cggcaaaaaa agtggtgaac agctatagca tcgaggagtt
     1381 aagcctgatt tttaaccagc ttggtgagtt acctgatggt aggcgttatg cccaggccat
     1441 atacgatgga cgtaaggtaa aacagctgga aaccatcgaa gagcttacac aaattttgaa
     1501 accgcttgtt ccagctaaac ttcaaaataa ggttttggcc aaaatctttc aggctttgcg
     1561 gattgaggta aaccatgagc tggatgcgct taaattaatg ttggttggct ctgctaaatg
     1621 tctcgggaga gaagggcggt tggtggttat ttcgtaccac tcgctggagg accgcctggt
     1681 aaaaaacttt ctcaagtttg gcaattttga gggagaggat agacccgatt taatgggtaa
     1741 cagggttgtc ccctttgagc aggtgaaccg gaaggtaatt gttccctcgt tcgatgagat
     1801 ttctgctaac aatagagcgc gtagcgccaa gttaagaata ggaagaaggc tatgagtgta
     1861 agtggcgaaa ctgaagaatt tgttgacatt caacctgaaa aacccaggga aaagaagggt
     1921 tttcggttta aggacttttt aagtgggcgg attctcaccc atgagaatgt ttcgtcacag
     1981 ctgccctaca tacttttttt ggttctcctt gccgtaatct acataggcaa tagctaccgg
     2041 aataaccagc tgttgcgaaa cgagcaaaaa attaaaattg aagtaaaaaa cctcagggca
     2101 gaatccatta caactgcggc caagtacatg tcagtaagcc gtcagtcgga ggtggtaaaa
     2161 cttgttgaaa gtaaggggct ggggcttgag gagtccacca tcccccctaa aaaaattgat
     2221 tagccaaaag cagcctgcag gtatgtcgat taagaaggac atagtaaccc gaattacagt
     2281 agtttacatt ctattcgcac tgctgggcgt tggtattttt gttaaaatca tctacataca
     2341 atttgttgag gggtcgaagc ttagggaaaa ggccaaagcc ataacctaca gggatattat
     2401 tgtggaacct aaccggggtg atattctggc atcggacggg agagtgctgg ccacttctgt
     2461 tccctactac gatattcgga tggatttggc agccagtggg ttgtccgatc aggtattttt
     2521 ctcaaatatt gattctctgg ctatttgcct atcccgattt tttggtgatc gttcaaaata
     2581 cgcatatcgt tcggagctgg ttaacgctcg caagtatgcc cataacaagc gattctaccc
     2641 aattgccccg cgcagggtga actacatgga gctgcaaact ataaaaacat ttccgttgtt
     2701 tcgactgggt gaaaataaag gtgggtttat cgcagttcag gctaaccggc gaattctgcc
     2761 acactccaaa atggctgcac gcaccatagg aggtgtcaac cagactggtg ttgccgtagg
     2821 cattgaggga gcattcgact atgcacttaa gggtaaggca gggcttaagg tgcatgaacg
     2881 tattcctggg aatagttggg ttgaggtgaa aagtatgaac cagatagacc cggaggatgg
     2941 ttgtgatgtg gttaccacca ttgatgttgc cctacaggat gttgctgaag cggcacttcg
     3001 taaacacctt ggaaatcacg atgccgacca tggttgcgct gttctcatgg aggtatcaac
     3061 cggcgatatt aaggcaattg caaatcttaa gcgcaacgag gatggtacct actctgaggt
     3121 ttacaactat gccattggcg aaagtactga gcccggctcc accattaaaa tagctaccct
     3181 cattaacctg ctggaagatg gctttgttga attgaacgat acggttaaca ccggcaacgg
     3241 caagttgcgg atgtacgacc gcataattac cgacagcaag gagggggggc atggtaaaat
     3301 ttcggttaag gaggtgtttg aagtttcatc aaacgttgga gtggccaagc tggttaccaa
     3361 gtactatagg ggaaaggaaa gtcattatgt tgacaggctc tactccatga agctcaacca
     3421 acccttagga attggcattc cgggcgaggc tagcccacag atcaagtacc ccaccgacag
     3481 gtactggtcg gggatgtcgt taccaatgat gagcataggg tacgagctca agcttacacc
     3541 gcttcaaatt cttacctttt acaatgctat tgccaacgac gggagaatgg ttaagccaag
     3601 gctggttaag tcgctagagc gacacggaat ggttgtacaa accttctcgc ccgaagttat
     3661 caacccctcc atttgcagca ggtcaaccct ccgaaaggtg aacgaggtgc tggaaggggt
     3721 agttgagagc ggcactgccc agaatcttaa aagtactccc tataaaattg caggcaaaac
     3781 gggaacagca cagcttgcca aggggcgaag gggctacaaa attggcggta gcgttagcta
     3841 tcaggcatcg tttgtgggtt attttccttc ggacaagcct agatattcct gcatagtggt
     3901 ggttaacgca ccttcgcaga atatctacta tggaaacctg gttgccgggc caatatttaa
     3961 agaaatttcc gacaaggtgt atgctaccac gcccgattgg ttcagggaga ttgataacgg
     4021 tggaagccta aaggagctcc ccgaatctaa atcaacccga ttggtaatgc taaaggaaat
     4081 attgaagcag ctcgatgtgc cttatcaaat tgctaacagt aaagctgaat gggtttcaac
     4141 caaacgcgat agcaacgaga ttgcgcttaa cacgcttagt ttttcatcaa aagctgttcc
     4201 gtcggttgtt gggatggggc ttagggatgc aatttttctt ctcgaaaaca ccgggttgca
     4261 tgttagcttt aacgggaggg gtactgttcg ttcgcaatcc attgccccgg gaacagccat
     4321 taagcgggga agcaacatca accttgaact tagtatgagc aattagaatt tatatatcat
     4381 ggcagtaggc ttaactaaaa tattaccaaa agcgcttgtc aacgaagtag ttggaaaccc
     4441 cgatatcgag gtggaacagc taaccttcaa ctcgagggtt gccggggcca actcttgctt
     4501 ttttgccata aggggaactc aggctgatgg gcaccggttc atccctcaag cagtcgaaaa
     4561 cggggcttca gctattgtgt gcgaagcaat gcccgttagc tttgccccaa atgtaacata
     4621 cgttagggtt tcggattcct cggaagccct gggcctaatg gcttctgcat tctacggtaa
     4681 cccgtcgtcg aaccttaagc tggtaggtgt taccggaacc aatggtaaaa ccaccacggc
     4741 aactctactt taccgaattg ttgggatgct gggatttaag gcaggcttgc tctcaacagt
     4801 ggtgaaccaa gtggtagacg agaaaatacc cacaacgcat acaactcccg acccaattga
     4861 attgaacagg ttactacgcc gtatggttga tgcggggtgt agctactgct tcatggaggt
     4921 gagctcacat gccgttgtgc agaaaaggat tgccggcctt acttttgccg gcggaatatt
     4981 caccaacatt acccacgatc atctcgacta ccataaaaca tttgacgagt acatcagggc
     5041 taaaaaaatg ttcttcgacc agctgccttc cgaatcattt gccctggtta atgccgacga
     5101 tcggaatgga atggtcatgg ttcagaacac caaagccaaa gtctctactt acgcccttca
     5161 ctccattgca gatttccgct gcagggtggt tgaaagccac ttcgatggaa tgctgctgaa
     5221 tatggatggg ctggaggttt ggacccggct tatcggtgag tttaacgcct ataacctgct
     5281 ggctatttat gcctcgcttt tgctacttgg gtttgacaaa accgatgtat tgctccagct
     5341 aagcatggtt aattcggtta gcggccgctt tgagtatgtt agctcaagtg gcggtatcac
     5401 cgcagtggtg gactatgccc acacccccga tgctctggtt aacgtaattg aaactataaa
     5461 aaagattaag agtagcggac aacggcttat aacggtggtc ggggctggtg gaaaccgcga
     5521 taagactaag agacctgtca tggcaagagt ggctgttcag aacagcgact tggtcattct
     5581 tacttccgat aatccccgtt ttgaggagcc cgaggatatt attaatgata tgaaggccgg
     5641 tgttgatgag gagtttacca agaagctagt taccattgta gatagacatg aagccatcag
     5701 gacggcatgc ctgctggcca acaaggaaga catcattctg gttgcaggca aaggccatga
     5761 aaactaccag gaggttaaag gggtaaagca ccactttgac gataaggaag ttttagctga
     5821 aatatttaac gagctgcata tatgatttac tacctggtac agtacattca aaagcaattc
     5881 gatttccccg gagccggtct tttccgctac atttcgttcc gctcggccct ggcttttatc
     5941 ctgtcccttc tttttgccct tttcattggc aagcgaatca tcaggtacct gcaacgaaag
     6001 cagattggtg aggagatacg caaccttggg cttgaggggc agctgcagaa aaagggaaca
     6061 cccaccatgg ggggaatcat cgtcattgct gccaccctga tctcggttct gctttttgcc
     6121 gatctcctta acatttacat catcataatg ctggtgacca ccgtttggat gggtgcagtt
     6181 ggctttaccg acgactatat caaagtattc ctgaaaaaca aggatggcct ttcgggtaag
     6241 ttcaaaattt taggacaagt tctactgggg ctatttgttg gtctcactat gtggctcagc
     6301 cccgacatta tgattcgcga ccatgtaaga ccaaatattc aaagcgaagt ggtggttacc
     6361 tcggagtact ccggttcgga acccgttagc gttagtaagg gcaaaaaaac aacccaaacc
     6421 accataccct tctttaagaa caacgagttt gactacgcgt acctaaaccc tttcaagggg
     6481 aagtggcgcg atgccatcac ctggactatc ttcattgtag ttgttatttt cattattact
     6541 gcagtttcaa atggcacaaa cctcaccgat gggcttgacg ggctggctac tggcgtttcg
     6601 gcaatgaatg ccaccacact tggggttttt gcctacctct cgggtaacgt tatttactct
     6661 gagtatctca acatcatgta tataccgcac attagtgagt tggttgtttt catgagcgcg
     6721 ctcatagggg cgcttattgg atttctctgg tacaacagct accccgccca ggtgtttatg
     6781 ggcgataccg ggagccttac cataggtggc attatagccg tttatgccat tgttgttcgt
     6841 aaggaactcc tgctgcccat actctgtgga atctttctgg ttgaagcgct ttcagttctc
     6901 atccaggtca gctactttaa ggttacccgg aaaatttacg gggagggacg aagggtattt
     6961 ctcatgtcgc ctctccacca ccactaccag aaaaagggtt atgccgaacc taaaattgtt
     7021 accaggtttt ggattataag catgttgctg gctgttatca ccgtggtaac tttaaaaatc
     7081 aggtagctat atgtgtttag ctggtgcaaa tatggggcta aatggtatca tacggtataa
     7141 aaaggttaac gtagggagga taccctacaa ttggctaccg tggcagatgg ctgcagctag
     7201 ccggaaacga atgaatacaa atatttcttt aaaatgaagc gaatagctat tctgggaggt
     7261 ggagagagcg gtgccggagc ggctgtgctg gcattcaaga agggctttga tgtttttcta
     7321 tcggattcat caaccataaa gccccgctac cgtgagtggc tgcaaaggta cagcatacct
     7381 tttgaggaaa acggccacac agaggcgctc attcttaacg ccgacgaggt gattaaaagt
     7441 cccggcattc ccgataaggc ttcaattgtt gcaagaatta gggagcaggg tatccctgtt
     7501 atttcggaga ttgagtttgc cggacgatac aacacagccc gaaccatctg cataaccggc
     7561 agcaatggca aaaccaccac tacctcgttg atataccaca tgatgcagaa ggcggggcta
     7621 aatgtgggcc tggctggaaa cataggcaac agttttgcct accaggtggc cgaagacaat
     7681 ttcgactact acgttattga gctcagcagc ttccagctcg atggcgttta cgagttcaaa
     7741 cccgacattg ccattctgct gaacatcacc cctgatcatc tcgaccgata cgactacaag
     7801 atgcagaact atgtggaatc gaagttcaga atcacccgaa acctatcaga ggatgattgc
     7861 ttcatttttt gttccgacga tgccgttacc gttgagcagc tcaaacgcat tatcctcaga
     7921 gcccagcagc tgcccttttc ccagcgtgtt aaggaaaacc aagctgcatg gctcgacgat
     7981 gataacctac taatcaacta taacaatagc gacttcagca tgtcaattaa cgatctttcg
     8041 cttaagggta agcataacat ctacaactct atggccgcag gcattgccgg gcatgtgctg
     8101 aacatccgta aggatgtaat ccggcagtcg ctatccgatt ttcagggggt agagcatcgc
     8161 cttgagccgg ttttaactat taaaggagtc aggtacatca acgattcaaa ggccacaaac
     8221 gttaactcgg cttggtatgc tctggagagt atggatacgc ccgtggtatg gattgccggc
     8281 ggggttgaca agggaaacga ttactcagag ctaatggact tggtaaagtt gaaggttaag
     8341 gcaatcgttt gccttggtgt tgataacgtc aagctgcata aggcttttgg gaatgttgtc
     8401 ccggttatgg ttgatgtgct gtcggctgaa gatgccgtta gaagtgccta cgagctggcc
     8461 aatccgggcg acacggttct gctttcacct gcctgtgcca gcttcgattt gtttgaaaac
     8521 tatgaggata gagggcgtaa attcaaggat gcagttagaa tgctttaagc attataccaa
     8581 tggggacttt tgtagataaa tactttaagg gcgatagggc aatatggctg gtggtaattc
     8641 tgttatcggc attctccctg ctggtagttt acagctcaac gggctcgctg gcctttcgtt
     8701 accatggcgg caacaccacc tactttttgg taaagcagct tgtttttttg gtgctggccc
     8761 tatcaattgt atttgttacc catctgatta gctatagggt ttactacccg ctgtcgtcgc
     8821 ttcttatgct tatttccatt cccctgttgc tctttacgct ggtatttgga accaggttga
     8881 atgaagcctc ccgctggctc gaaatacctg ggctgggcat aacattccag tcgtcggatg
     8941 tggctaagtt tgccctggtg atgtacatgg ccaaaatact ttctcagaag caaaaggata
     9001 taaaggacct taagcaggga tttttaccgc ttgcacttcc cgtaatcctg atttgtgggc
     9061 ttattcttcc tgccaacttt tccaccgctg cacttttggg gcttacatgc tggattatga
     9121 tgtatgttgg gagggtaaat ctgaaatatc ttctcgggtt tacaggaatt ggtgttgccc
     9181 tttttgccat ttttgttgtg gtagcccttc attttggcga tgtgggtagg gtacatacat
     9241 ggaaaaaccg tatcgaaaat tttgttagtg gcgataaccc cgacggtaac taccaggttg
     9301 agcaatcgaa aattgccatt gccaacggcg gtatcaccgg taaagggccg ggcaggtcaa
     9361 cccagagaaa tttcctaccc catccgtact ccgattttat atacgccatc atcattgagg
     9421 agtatggact ttttgctggg ctgttcatca tcagtctata cttaattctt ctataccgct
     9481 cggcggtgat agtcaaaaag gccaagcgta cttttccggc tttcctggct tttggtttgg
     9541 cgcttctcct caccatgcag gccatggtga atatggctgt ggcggtaaac cttattcctg
     9601 ttaccgggca accgctccca atgattagca tgggaggaac ctcgctgttt ttttcaagcg
     9661 ctgcgtttgg tattattttg agtgtaagta ggttacaaag taaaaaggaa ctttttgatg
     9721 gagaagaacc agctgaaagt aataattagc ggcgggggaa ccggcggcca tattttcccg
     9781 gctattgcca ttgccaacgc cgtgaaagag ctgtcgcccg aggccgaaat tctctttgta
     9841 ggagcgttgg gccgtatgga gatggaaaga gttccgcagg caggataccg tattgttggc
     9901 ctgcccataa tcggcattag acgaagcctt tcaccccaga atattatgct ggtttttaag
     9961 ctgattagaa gtcttcgctt gtctcgtaaa attatagccg actttagacc cgatgtggtg
    10021 gttggtgtag gcggctatgc cagcgggccc attctcaggg cagctcaaaa gcgagggata
    10081 c
//
LOCUS       DAOPHX010000097         9854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_8474_length_9854_cov_6.640882, whole genome shotgun sequence.
ACCESSION   DAOPHX010000097 DAOPHX010000000
VERSION     DAOPHX010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9854
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8474_length_9854_cov_6.640882"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(301..1527)
                     /locus_tag="PK502_07830"
     CDS             complement(301..1527)
                     /locus_tag="PK502_07830"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPH00764.1"
                     /translation="MNIEEYLAGFSLSGSAGIKTQVLDLPVRGSTYPVFINEYWTSKQ
                     RQASSIHEISYRACFKPQLPHFFIERLTSEDETVYDPFLGRGTTIIEAALMGRNVAGN
                     DVNPLSLMLTRPRLFIPNLADVQQRLSSIPVNSSLRASSDLSMFFHPQTEAEIVSLRN
                     YLDTRKQQEKEDEVDQWIRMVATNRLTGHSVNFFSGYTLPPNQAISAEKQRMLNIKHG
                     KTPGYKNVKSIILKKSGDLLRNISEEETSRLRKAGEKAILLNTDARHANTLPDNSVSL
                     TITSPPFLNVVQYAADNWLRCWFNNINIQDVASRITMSKTIEEWENVMREVFVMLYSK
                     TKHKGYVAFEVGEVRNGKVKLENHVVPLGLEAGFDCVGILINQQKFTKTSNIWGVSNN
                     SRGTNTNRIVIFRKSK"
     gene            complement(1714..2691)
                     /locus_tag="PK502_07835"
     CDS             complement(1714..2691)
                     /locus_tag="PK502_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HPH00765.1"
                     /translation="MDQNKPISSFIEKHYLHFNAASLVDAAKAYKKHLADGKKMMITL
                     GGAMSTAELGISLAEMIRQDKVQIITCTGANLEEDLMNLVAHNHYKRVPDYRDFTPQQ
                     EWDLLQSGLNRVTDTCIPEEEAFRRLQSHLFDIWKSALDKGERFFPYEYMYKLIRSGV
                     LKQYYEIDPKNSWMLAAAEKNLPIIVPGWEDSTMGNIFAAYCIKNELKPSIMKTGIEY
                     MMFLADWYTKNAGKEGIGFFQIGGGISGDFPICVVPMLTQDLEREDTPFWSYFCQISD
                     STTSYGSYSGAVPNEKITWGKLGINTPKFIIESDATIVAPLIFAYVLGW"
     gene            complement(2715..3158)
                     /gene="rpiB"
                     /locus_tag="PK502_07840"
     CDS             complement(2715..3158)
                     /gene="rpiB"
                     /locus_tag="PK502_07840"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPH00766.1"
                     /translation="MEKEKLAIASDHAGYHLKEDLKQFLGELGYHVWDFGTHSEDSVD
                     YPDFAHPLAQAVENGEYRFGFLLCGSGNGVSITANKHQGIRAALCWIPEIASLARMHN
                     DANVCSIPARFVSAQEAKLIAKAFLDARFEGGRHSRRVEKIPLSK"
     gene            3288..4430
                     /gene="hemW"
                     /locus_tag="PK502_07845"
     CDS             3288..4430
                     /gene="hemW"
                     /locus_tag="PK502_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPH00767.1"
                     /translation="MAGIYVHIPFCRRKCLYCDFYSVGERFITGDFDKLINHELLLRG
                     NFLNDKRISTVYLGGGTPSLLPIESVGRILEGIDTLSSLNGDAEITIEVNPDDVSLEL
                     FRSYKRLGVNRISIGVQSFNQAELSFLGRRHSVESSSVAVHNAQDAGFENISIDLIYG
                     IPGSTLQTWEHSLSVAENLNPQHLSCYHLTYEGNTPLTRRLRKGLFTALDEELSLGQF
                     ELLRSFAQSKGYLHYEVSNFAKEGFISRHNSSYWLGEEYLGLGPAAHSYNGAVRQWNP
                     YSLERWEDGIKNNSPNIEMESLSAVSKYNELLITRLRTIWGVNTDDVSRMFGESFASF
                     LNKSAQKYIDDGRMSRTKGNTLLISPSAYFVSDSIVENLMLIAEPD"
     gene            4527..7649
                     /locus_tag="PK502_07850"
     CDS             4527..7649
                     /locus_tag="PK502_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPH00768.1"
                     /translation="MKLVILLVFVIIHRNTFRFWIILTVAFIAVTALTIVPLVLAKKH
                     YAELIRREFDANIQEIHNRSTGYFQTIENGIANNLTLETYNDPLVSLKFTTDSYVRVQ
                     NVRREGKTVYSSPSDDNSEFYLFSESKVFPFEVATAVKRSDGFWGMKSFGFESYNVDY
                     LFRFDNLLSYLQGLTACNICMTLGDIQINPRTGSNSAAFRWNPYVLESGKWPAVVAQN
                     KQYFYVVNLAEVVDVSGNKAGMTFLAVDVSEIHRLFVSLVGLVSILGALSILLLGLFA
                     VKRYRFLKTAAKHEDNIKTNEEDLLRRASLVNLFDAMENGVVMINTDFEVVYINESYA
                     DFTGLQHSTTVGEKCYSALQGMYCHTAECPLSRILAGEPRVETTEFRFKSNGEKARFA
                     VATNPITDDSGKILGIIENFKDITEKLEVEQTLKQTEEQFEIFMNSFPLGVFIEDGIT
                     HEIIYQNAYLNDISNGACIKDFLPKEKSVKPVKSYRESTEEVMLVDSSGHWRYFLLHK
                     FKFIGVGNNLRIGGIMIDTTERREVERYRDVLSEAIDNSPVSIVILSPATEIEFVNPY
                     FLELTGYRIEEVYSKDINSLDLEYNSVKVLGKAVAAALGGRVWQGEVHLKHRNIGHSW
                     VSASFIPITDSTGSLQNIVGVMENITQHKEYEKEILLAKSKAEESDNLKSAFLSHLSH
                     EIRTPLNAIIGFSSLLSDSDLSLTERRNLSEIIYRNSNDLLRLIENLIEISEIETGTL
                     DIKKSQFSVNALMHSLYLQILEEDKKATNVRFSLRREVHHDDMVIYSDEAHIRQVLFH
                     LLVNSCKFTNHGFIEFGYSIVDEFTIMFYVLDSGVGIETDKQALIFNPFRQVDESSTR
                     QFGGMGLGLAISKHIVEKLGGKIWFNSTPGSGTNFYFTVPYIPVNSKFEVDNVGSRLQ
                     KFNWSSKTILVADDIDVNFVYLKAALKRTNAQVLWAKNGKEAVEIVRQGPESINLVLM
                     DLVMPDMDGFEATRLIKDFDSSIPVIGQTAYPNPGNAEAVNQYGLDSLLEKPIKINSL
                     LNELNKYLDN"
     gene            complement(7663..8997)
                     /locus_tag="PK502_07855"
     CDS             complement(7663..8997)
                     /locus_tag="PK502_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPH00769.1"
                     /translation="MQTLTATPNMVGMAVLLGMLLVIGLLLYRNKQLKNNNKELLATI
                     RQLQTMNARQEEISATLTDKLNDTLQKLSELTECKTSFWVDLNHRMRTSLNGLLGFSQ
                     ALLMNNTTKKEKDAAAQQIQQLCNKIILLADEMVDLAKIEANQLEVLPASCNINKLIS
                     DILHQLTENREYMSRQVALKLALSLDDEQAIMVTDADKLKKTLLHILGKLSKLAESDT
                     LEIGYRVDSNASITFHVREVKQPFTDTSSNEIILRQSVRSNGKHFTRQQGEAEIGMII
                     ANGYAKALGGELDFGSTPDAGNVVYLRLPLNISKKKQSNELFCSPENWSGKTILVVED
                     DLMNFLYIEALLKRTNVRLIHAKNGEDAVEITLLHPEISLILMDLRLPFMDGYRVVRE
                     IRKQNHDIPIVATTASSMANEEQRSLDAGCNAFITKPLDSDELYKTIRRYIR"
     gene            complement(9387..>9854)
                     /locus_tag="PK502_07860"
     CDS             complement(9387..>9854)
                     /locus_tag="PK502_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HPH00770.1"
                     /translation="TNPAAVDKIFRLKQRDDSKSMIVLVDQPGRIPSYVDEVPEVAWD
                     LVELADKPLTIIFQKAKNLAPNLIAPDGSIGIRVVRHDFCQKLILQFGKPIVSTSANI
                     SGRPFPKNFSEVDEVIKSGVDFIVPQTYDHPVSDKPSGIIALGDGGLVRIIRE"
BASE COUNT         2565 a         2127 c         2341 g         2821 t
ORIGIN      
        1 ggtagggctt tgctcaggat cttattcatc tgcttaagtt tatgttactg acactacagc
       61 tggttgtaaa agtaaatgaa cctagaaaac ttatgtttct acttgcttac cataccgagc
      121 tatattccct atgaattaaa atgaattttg aagcagttca aaatttaccc atgggtagct
      181 caacagttta gcgttcaaat atacgcaaaa attacggtga tgggttaaaa tcatatttga
      241 aatggctgaa cagtagttct tttaggtatg atgctttact aaaaattttt tcaatgtggg
      301 ctatttggat ttcctgaaaa tcacaattct gttggtgtta gtgccccttg agttgttgct
      361 aaccccccaa atatttgatg ttttagtaaa tttctgttgg ttgattagta ttcccacgca
      421 gtcaaaaccg gcttcgaggc ctaaggggac tacatggttc tccagcttta ccttaccatt
      481 tctaacttca ccaacctcaa atgccacgta gcccttgtgt ttagttttgg agtaaagcat
      541 cacgaatacc tcacgcatca cattttccca ctcctcaatg gttttagaca tggtaattcg
      601 ggatgcaaca tcctgaatat tgatgttgtt aaaccagcat cgaagccagt tgtcggctgc
      661 atactgcacc acgttaagga agggcgggga ggttatggta aggctaaccg agttatcggg
      721 cagggtgttg gcatggcgtg catcggtgtt caggagaatg gccttttccc ccgccttgcg
      781 caggcggctg gtttcttcct cggatatatt ccgcagtaaa tcccccgatt ttttcagaat
      841 gattgactta acgttcttgt agccaggagt tttaccatgt ttaatgttca gcattctttg
      901 tttttcggca cttatggcct ggttgggcgg tagcgtgtag cccgaaaaga agttgaccga
      961 atgacccgta agcctgttgg ttgccaccat tcgtatccac tggtccacct catcctcctt
     1021 ttcttgttgc tttcttgtgt ccaggtaatt acgcaagctc actatttctg cttcggtctg
     1081 ggggtgaaaa aacatactta agtcggaact tgctcttaag gaagaattaa cgggtatgct
     1141 ggaaagtcgt tgctgcacat cggcaaggtt tgggataaac aacctgggtc gggtgagcat
     1201 aaggctcaac gggtttacat cgttgcctgc aacgttacgg cccatgagag cagcttcaat
     1261 aatggttgtt ccccgcccca gaaatggatc gtaaacagtt tcgtcctcgg aggtcaggcg
     1321 ttcaatgaaa aagtgcggaa gttggggttt aaaacaagcc cggtaggaga tttcgtggat
     1381 ggagcttgcc tgccgctgtt ttgatgtcca atactcgttg ataaatacag ggtatgttga
     1441 gcccctaacc ggtaagtcta acacctgggt ttttatgccg gcactgccgg aaagggagaa
     1501 tcctgccaga tattcttcta tgttcaaggt ttagctactt tacaactgca caaatttagg
     1561 aaggaatgac cacaagaaaa agcatggggg tgcatcgggg gaatatttga ctttttggct
     1621 tgccagggtt atgttcaaaa gcaaggccgg agagttctcc ccggccaagc caacatctat
     1681 tgtattgctg cgcaatggag ctgagttcac cttctaccaa cccagcacgt aggcaaatat
     1741 taggggggca acaatagtag cgtccgattc gatgatgaat tttggggtgt tgatgcccag
     1801 ctttccccag gtgatttttt catttggaac tgctccggag tatgacccgt agctggtggt
     1861 ggaatcgcta atctggcaga agtagctcca gaaaggtgta tcctctcgtt ccaggtcctg
     1921 tgtaagcatg ggaaccacgc aaattgggaa atcacctgaa attcctccgc caatttggaa
     1981 gaagccaatg ccctctttcc cggcattttt ggtgtaccaa tcggccagga acatcatgta
     2041 ctctatacca gttttcatga tgctgggttt aagctcgttt ttaatacagt aggcggcaaa
     2101 aatattgccc atggtagagt cctcccagcc tggaacaata attggcaggt ttttttcggc
     2161 agcagcaagc atccatgagt ttttgggatc aatctcatag tactgcttca gcacaccgct
     2221 acgaattagc ttgtacatgt actcataggg gaaaaaacgt tcccccttgt cgagggcgct
     2281 cttccaaatg tcgaagaggt gtgattgcag cctcctgaat gcctcctcct cggggataca
     2341 ggtatctgta accctgttaa ggccgctttg aagcaaatcc cattcctgtt gtggagtaaa
     2401 atcgcggtaa tcgggaactc gtttgtagtg gttgtgggcc accaggttca tcaagtcttc
     2461 ctcaaggttt gccccggtac aggttataat ctgaacctta tcctgcctta tcatttcggc
     2521 caggcttata ccaagctcgg cagtgctcat ggcgccgcca agggtgatca tcatcttctt
     2581 gccatcggca aggtgttttt tgtaagcctt agctgcgtca acaagtgaag cagcgttgaa
     2641 atgcaggtaa tgcttctcga taaacgagga gattggtttg ttctgatcca ttttcttaga
     2701 atattgttgg gttgttattt ggatagtgga attttttcaa ctctacggga atgccggcca
     2761 ccttcaaacc gggcatccag aaaggctttt gcaattagct tagcttcctg agccgaaaca
     2821 aatcgggccg gaattgagca aacgttggca tcgttatgca tacgggcaag gctggcaatt
     2881 tcagggatcc agcaaagtgc agccctaatt ccctggtgct tgttggcagt tatcgatacg
     2941 ccattcccgc ttccgcaaag caagaatcca aaacgatatt cgccattctc gaccgcttgt
     3001 gccagcggat gggcaaaatc gggatagtca acgctatcct cgctgtgcgt tccaaaatcc
     3061 caaacatgat atcccaattc cccaagaaac tgctttaaat cctctttcag gtggtaacct
     3121 gcatggtcgg aggcgatggc tagcttttct ttttccatgt tgccgggtat aattaccttt
     3181 tctgccaata gatgaaatgg tttgtgttcg tataacgtta actttgcaaa gataatggct
     3241 ttttggcttt tatatctgtt tttgtgtcgt aatcatttgg tgacaaggtg gctggtatat
     3301 acgtgcatat tcccttctgc aggcgcaagt gcctctactg tgatttttac tcggtggggg
     3361 aaaggtttat taccggtgat tttgacaagc ttatcaacca tgaactgctg ctacgaggaa
     3421 acttcttaaa cgacaagcgc atcagcactg tttaccttgg cgggggaact ccctccctgc
     3481 ttcccattga aagtgtcggg aggatacttg aaggcattga tactcttagc agcctgaatg
     3541 gtgatgctga aatcaccatt gaggtaaacc cagatgacgt ttcgttggaa ctttttcgga
     3601 gctataagag gctgggtgta aacaggataa gtataggcgt gcagtcgttc aaccaggcag
     3661 aacttagctt tctggggcga cggcatagcg ttgaatcctc ctcggttgct gtgcataatg
     3721 ctcaggatgc aggttttgaa aacatcagca tagatttgat atacggcatt cccggctcaa
     3781 cgcttcaaac ctgggagcat tcactaagtg tggcagaaaa tttgaatccc caacaccttt
     3841 cgtgctacca ccttacgtat gaagggaaca ccccgcttac ccgtaggtta aggaagggat
     3901 tgtttaccgc ccttgatgag gagttgagcc ttgggcagtt cgaactcctt cgtagttttg
     3961 cccaatcaaa gggctacctc cactacgagg tctctaattt tgccaaggaa ggatttattt
     4021 ccaggcacaa tagctcctac tggcttggtg aggagtactt aggccttgga ccggcagccc
     4081 attcctacaa tggagctgta aggcagtgga atccttactc gctagagcgc tgggaagacg
     4141 gaataaagaa taattcgcca aatattgaaa tggaaagcct tagtgctgtt tcaaagtata
     4201 atgagctgct cattaccagg ttacgaacca tttggggtgt gaatacggat gatgtttccc
     4261 gcatgtttgg agaaagtttc gccagctttt taaacaagtc tgcccaaaag tatatagatg
     4321 acgggaggat gagccgaacc aaaggcaata cactcctgat aagcccgtcg gcctactttg
     4381 taagtgatag cattgtggag aatttaatgt tgatagcgga gccagattag gacttgctct
     4441 acgcctgcaa cccgaagatg tggtgaacaa tcttttcgta aaagtattag gcagtgatgt
     4501 ggctaatgaa ataaatattc atatttttga agctagtaat attgctggtt tttgtgatta
     4561 ttcatagaaa tacattccga ttctggatta tacttacggt tgcctttatt gctgtaaccg
     4621 ccctgacaat tgtcccgctt gttttggcca aaaagcacta cgcagagcta atacgtagag
     4681 agtttgacgc caatattcag gaaatccaca accgttctac tggttacttt caaaccattg
     4741 agaacggcat tgccaataat ttaacgcttg aaacgtataa tgacccgctg gtatcgctaa
     4801 aatttacaac cgattcctat gttcgggtac aaaatgttag gcgtgaaggc aaaacagtgt
     4861 atagtagccc atcagatgac aattcggaat tctacctgtt ttctgaaagc aaagtatttc
     4921 catttgaggt tgccacagcg gttaagcgtt ccgatggttt ttgggggatg aagtcgttcg
     4981 gcttcgagag ctacaatgtt gactatttat tccgattcga caaccttctc tcatacctgc
     5041 aggggttaac cgcatgcaat atctgcatga ctttaggaga tattcaaatc aacccaagaa
     5101 ccggaagtaa ttcagctgca tttagatgga atccttatgt tctggagtct ggcaaatggc
     5161 ctgctgttgt agcgcagaat aaacaatact tctacgtggt caatcttgct gaagtagttg
     5221 atgtatcagg gaacaaagcc ggaatgacat ttttagcagt tgatgttagc gaaattcaca
     5281 ggcttttcgt gtcgctggtt ggcttggtgt caatattggg agcgttatct atcctgctgt
     5341 tagggctgtt tgcagtaaaa cgataccgtt ttctaaaaac cgcagccaaa cacgaggaca
     5401 atatcaaaac aaatgaagaa gatttactcc gcagggcatc gttggtgaac cttttcgacg
     5461 caatggaaaa tggggtggta atgattaaca ccgattttga ggttgtttac attaatgaaa
     5521 gctatgctga ttttacgggt ttacaacaca gcacaacggt aggggaaaaa tgctacagtg
     5581 cacttcaggg gatgtactgt catactgccg aatgcccgtt gtcgcgaata ctggccggtg
     5641 agccaagggt tgaaacaacc gagttccggt ttaagtcaaa tggcgaaaag gcacgatttg
     5701 ctgtagccac taatccaatt accgatgata gcggcaaaat tctagggatt attgagaatt
     5761 ttaaggacat caccgaaaag ttggaggtgg aacagaccct aaagcaaacc gaggagcagt
     5821 ttgaaatttt catgaactcg tttcccttag gcgtttttat tgaggatgga attacccatg
     5881 aaatcatata ccagaatgcc tacctgaatg acatatcaaa tggggcatgc attaaggatt
     5941 tcttgccaaa ggaaaaaagt gtaaaaccgg ttaaatcgta cagggaaagt accgaggagg
     6001 ttatgctggt tgattcatcg gggcactggc ggtatttcct tcttcataag tttaaattta
     6061 ttggcgtggg caacaacctt cgtattgggg ggataatgat tgatacaact gagaggcgag
     6121 aggtggagag gtaccgcgat gtgctctcgg aagcgattga caattcgcct gtaagcattg
     6181 taatcctttc accagctacc gaaatagagt ttgtaaaccc atacttcctg gaactaaccg
     6241 gatataggat agaggaggtt tacagcaagg acatcaacag cctcgacctg gagtataact
     6301 ctgttaaagt actcggcaaa gctgttgccg ctgctttagg aggcagggta tggcagggcg
     6361 aagttcacct caagcatcga aatattggcc atagctgggt tagcgcttca tttatcccca
     6421 taaccgattc tacaggcagt ctgcaaaata ttgttggtgt tatggagaat atcacccagc
     6481 acaaggagta cgaaaaggaa atactgctgg caaaaagtaa agccgaggaa tccgataacc
     6541 ttaaatcggc ttttctttcc catctttcac atgaaataag aacacccctg aatgccatca
     6601 tcggtttttc atccctgctt tccgacagcg acctcagcct aaccgaacga cgaaaccttt
     6661 cggaaataat ctaccgtaac tccaacgatt tgctcaggtt gattgaaaat ctcattgaaa
     6721 tttccgaaat cgaaaccgga acgcttgata ttaaaaagtc ccagttctcg gttaatgccc
     6781 tcatgcactc gctgtacctc cagattctgg aggaggataa aaaagccacc aacgttcgtt
     6841 tcagccttcg tcgggaggtg caccacgacg atatggttat atacagcgat gaggcgcata
     6901 tacgacaggt ccttttccac ctgctggtca actcctgcaa gttcaccaac catggtttca
     6961 tcgagttcgg atactccatt gtggatgagt ttaccatcat gttctacgtc ctggatagcg
     7021 gcgttgggat tgaaaccgat aaacaagctc tcatcttcaa cccctttcgc caggtagatg
     7081 agtcgagcac ccggcaattc ggtggtatgg gcttaggcct ggccatttcc aagcatattg
     7141 ttgaaaagct gggcggtaaa atatggttca acagcacccc gggatcggga acaaactttt
     7201 acttcaccgt gccctacatt ccggttaact ccaagtttga ggtggacaac gtgggcagcc
     7261 gcctgcaaaa attcaactgg agctctaaaa ctattctggt agccgacgat attgatgtta
     7321 actttgttta cctcaaggca gcgcttaaaa ggacaaacgc ccaggtttta tgggcaaaaa
     7381 acggtaagga agccgtagaa atagtaagac agggtccgga gagcataaac ctggtgttga
     7441 tggatctcgt aatgcccgat atggatgggt ttgaagccac gcggcttatc aaggattttg
     7501 acagcagcat tccggtaatc ggacaaacgg cataccccaa cccggggaat gctgaggcgg
     7561 taaaccagta tggacttgat tcgctgctcg agaagccaat caaaatcaac tccctgctca
     7621 acgagctcaa caagtatctg gataactaac cctggttgta agctaccgga tgtacctcct
     7681 tattgtttta tatagctcgt cggaatcgag aggctttgtg atgaatgcgt tgcatccggc
     7741 atctaagcta cgctgctctt cattagccat gctgctggct gttgtggcaa ctatcggaat
     7801 gtcatgattt tgttttctaa tttcccggac aaccctatat ccatccataa atgggagtcg
     7861 aagatccatg agtataaggc ttatttccgg atgaagtagg gtaatttcaa ctgcatcctc
     7921 cccgttctta gcatgtatca acctcacgtt tgtgcgtttc agcagggctt cgatgtacag
     7981 aaaattcatg aggtcatcct caacaaccag aatggtttta ccactccaat tctcggggct
     8041 acaaaacaat tcgtttgact gtttcttttt tgaaatattc aggggtagtc gtaggtaaac
     8101 cacatttccc gcatcaggcg ttgagccaaa gtctaactct ccacccaacg ccttggcgta
     8161 accattggca attatcattc ctatttcggc ttcaccctgt tgtcttgtga aatgtttgcc
     8221 attagacctt actgattgtc taagaattat ttcgtttgat gatgtgtcgg taaagggctg
     8281 ctttacttcc cgaacatgga aggtaatgct tgcattggaa tcaactctgt agccaatttc
     8341 aagggtgtcg gattcggcaa gttttgataa ttttcccaga atgtgcagca gcgttttctt
     8401 gagcttatcg gcatcggtaa ccataatggc ctgctcgtca tcaagcgata gcgcaagttt
     8461 tagggctacc tgacggctca tgtactccct attttctgta agttggtgta aaatatcact
     8521 tattagctta ttaatgttgc agctggcggg gagaacttcg agttggttgg cttctatttt
     8581 ggctaggtca accatttcgt cggccagaag gataatttta ttgcaaagct gctggatttg
     8641 ttgagccgca gcatcctttt ccttcttggt ggtgttattc ataagaagtg cttgcgaaaa
     8701 tccaagcagc ccatttaggg atgtccgcat tctatggttc aaatcgaccc aaaagctggt
     8761 tttgcactcc gttagttcgc tcaacttttg gagggtatca ttcagcttgt cggtaagggt
     8821 tgcagaaatt tcttcctgcc tggcattcat ggtttgaagc tgccttatcg tcgccagtaa
     8881 ctccttgtta ttatttttta attgtttgtt gcggtagagc agaaggccta tcacgagcaa
     8941 cattcccaac aaaacggcca tgcccaccat gttgggggtt gccgttaagg tttgcaaaag
     9001 gatgtttaat ttatccggta tcattcagga tacgccattt gcaattaaaa ttgttgagaa
     9061 atttgctgtt attttaaggc cgaatagccg ctaccattca actttggggt tataagtcaa
     9121 cataaagttt gactgctaga ggtattaaca ggggattgct aaatgattcg gagcgaatgg
     9181 atatggtttt tagggtgcta gctgtgggca aaagtaactt tcgttcaagt cattatcaaa
     9241 acgcccatct ggtttttagg cgaaacacgt caaaaatttg atagaacttg atttagtgga
     9301 gattaacttt tcgttactcc tgtcgggatg gcaagaagac ctgatttttg gcttatatag
     9361 ttgattatct gcccataacg gtgtagctac tccctgataa tccggacaag tccgccatcg
     9421 cccagggcta taataccaga aggtttgtcc gatacaggat ggtcgtaggt ttgcgggaca
     9481 atgaaatcca ctcccgattt gatcacctca tcaacttcgg agaagttttt ggggaatggc
     9541 cgtccggata tgttggcaga ggttgacaca atgggctttc cgaactgcag gattagcttc
     9601 tggcagaaat cgtgccttac caccctaatg ccgatgcttc cgtcgggggc aatgaggttg
     9661 ggcgccagat tttttgcctt ttggaagatg atggtcaggg gtttatcggc cagctcaacc
     9721 aggtcccagg ctacttcagg tacctcgtca acgtaggatg gaattctgcc gggttggtca
     9781 accagcacaa tcatgctttt gctatcgtcg cgctgtttca acctgaatat tttgtcaacc
     9841 gctgcagggt ttgt
//
LOCUS       DAOPHX010000098         9745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_8634_length_9745_cov_5.170382, whole genome shotgun sequence.
ACCESSION   DAOPHX010000098 DAOPHX010000000
VERSION     DAOPHX010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 9745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9745
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8634_length_9745_cov_5.170382"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PK502_07865"
     CDS             <1..287
                     /locus_tag="PK502_07865"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPH00771.1"
                     /translation="LDGYMFDSNIDSVATRLGRSFSSTDSLKVTVGTGVIKGFSQALV
                     LMKKGEWGRVIMPSYCGYDSVGSTTIPPYSPLIFDLYFSSKSVVTKPQSK"
     gene            complement(279..986)
                     /locus_tag="PK502_07870"
     CDS             complement(279..986)
                     /locus_tag="PK502_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPH00772.1"
                     /translation="MAGKLYLVPTTIGSTSWNEVIPSGVVELTRGLKFFVVEELRTAR
                     RYLSALGIQTPIENLCFFTLNEHSSAADLTDMLKPLLDGNDVGLMSESGLPAIADPGS
                     MLVSAAHHHGIRVVPLCGPSSIIMTMMASGLNGQNFAFVGYLPVKSEERKRRIRQLEQ
                     RSRAENQTQLFIEAPYRNVALFRDILQTCADEARLTIAAGLTLEGEYIATKKIREWKQ
                     LPIPDINKINTVFALLF"
     gene            1170..1931
                     /locus_tag="PK502_07875"
     CDS             1170..1931
                     /locus_tag="PK502_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPH00773.1"
                     /translation="MKITFLGTGTAQGIPVIACSCPVCASTDSHDKRLRTSALIEEQG
                     INILLDAGPDFRQQMLNANVKNLNAILITHEHRDHVAGLDDVRAFNWVQRQPMDIWAE
                     DRVLEAIQREYAYIFAERKYPGIPEVELHQVFGHQFDILGVTVIPIRVYHCKLPIFGF
                     RIGDLTYITDGSFISEEEKDKIVGSRVLVINALRMQKSLSHFSLPEALKLVEELSPRK
                     AYITHISHQMGFYRDVARQLPDNVFLAYDGLVVEV"
     gene            complement(2013..4208)
                     /locus_tag="PK502_07880"
     CDS             complement(2013..4208)
                     /locus_tag="PK502_07880"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPH00774.1"
                     /translation="MKRNLVKLLLIAVITRFGVWSALADEGMWMVNLIHQNIATMQTM
                     GLKLTAEDIYSLNHSSLKDAIVQFNDGGCTGELVSAQGMFFTNHHCGVEAVQALSSEK
                     NNLLENGYWAGSLSEELPAHDQVALILVGVEDVTAKVFASLNAGLSNQEYFDKMREVM
                     QQLEDETSKKTGYHTIVKPFYNYNQFFMFTYERFLDVRLVGVPPSSIGNFGGDVDNWH
                     WPRHTGDFCIFRIYTAPDGKPAAYSPRNVPYKPKNFLKISLKGVNEGDFAMIMGYPGT
                     TYRYATSYEAQYSRDVVAPWKVDVWGNFISIVKQARSYNPKAKVAYTDKHDYLVNFYQ
                     KDVWQAQSMFRYNVVERLAAREDSLKAWVNQNPSLRSKYLPTIPVTRNFFEQATQNKW
                     EALENSLSALSFFPVDVYKNVNACNNLLGAIFEQNKPYSPLKFWKVDRIRREARVLKK
                     NIPGIFDSYFYEPDMRLYATAFGNLIANIGDCQNLGLLNAIKKVDNIDSLYPYYIQGF
                     YEKSYFTKAQNLEKLIKYPYMDSLLTDPLFILYHNYSALWDSIYPQMHNARMDFQKAM
                     QLYTKGLMEMNAGKLLYPDANSTMRLTYGRVTGYKPADGLYYKPFTTLDGVMEKENPN
                     EDVFTVDPKLRQLWQNKDYGRYASNGTIPVCFLTDNDITGGNSGSPVLDGNGNLIGLA
                     FDGNSEAMACDFMFEPETQRTIAVDIRYVLFIVDKFANAQHIIGELTTL"
     gene            complement(4521..4946)
                     /locus_tag="PK502_07885"
     CDS             complement(4521..4946)
                     /locus_tag="PK502_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00775.1"
                     /translation="MSGRLNKGLIFIVLLAAIPLSCEELNSWFVDCSQCFKDKISRTD
                     ITVKLTINDENPWVIFDVYRGNTDSGELLFTDTSVVAEYYWRAEINQFYSVKARYSCK
                     GRVIYVVDGAMLKTKLESSACDEKCYTIHGDVLDARLKY"
     gene            complement(4936..6357)
                     /locus_tag="PK502_07890"
     CDS             complement(4936..6357)
                     /locus_tag="PK502_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPH00776.1"
                     /translation="MSRQKSKIIRVIIQLFLLILPATWLWAQDDELLPTDTADFKSHY
                     NMLLIFEADSGHTDMVQYLLELGADPNTQTEQGVTPLMYASQWGYRDIAELLLKNGAN
                     PNLVPFDGNTALHASVKAGNDSIAELLLDYNAEVNVANRKGLTPLHYAVWYGFPYLTD
                     LLIYHGADVNAQDNHGNTPLMLAVYNGTNLCTTLLLESGANPDIQDNEGKSPLMVAAQ
                     FNDTLLSKLLLSYNADIKLHDKKNATALALAIANRSSQTIQLLLENNAGSYTLSKSYY
                     QLAAETGSREVLEILRKNGLKTKLKPSIGSIGVGTGFIFNNHEFLWGFEIEPVESISK
                     TGLSVGYWFRPSPVAVLYPKNNNLFQLHEEKQVVSVKLRHYIPIVFRPKGIGLKAQFG
                     PSFDWISRNTSGTVTNPPDVVRLGANAALNLHINSFEITVGWDYTWLKTLDASPHRAN
                     INIRYNLPIAKPRIVNKTIKYVR"
     gene            complement(6418..9144)
                     /gene="ppdK"
                     /locus_tag="PK502_07895"
     CDS             complement(6418..9144)
                     /gene="ppdK"
                     /locus_tag="PK502_07895"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPH00777.1"
                     /translation="MSKVKRVYTFGNKKAEGKADMKNLLGGKGANLAEMCLLGLPVPA
                     GFTITTDTCNEYYQNNHELPSDLIPDIWEAMKNIEKIMGMKFGDPENPLLVSCRSGAR
                     KSMPGMMDTVLNIGLCSSTIPGLIKKTNNPRFVWDSYRRLIMMYADVVMEKAEGVEPT
                     DGKGIRKLLDDILDEYKNSKGYKDDTQLTAEDLQHLAEEFKKQVKKSLGKEFPDDAKE
                     QLLGGIKAVFKSWNGKKAVSYRRIEGIPDDWGTAVNVQAMVFGNMGNSSATGVAFTRN
                     PATGENVFYGEWLINAQGEDVVAGIRTPNPLNNETKNDQNKHLPSMEELMPDTYKELV
                     AVRNTLEKSFKDMQDIEFTIQEGKLYMLQCRTGKRTGTAALNMAMDMLHEGLIDEKTV
                     VLRVEPAQLDELLHPICDPEEEKKVNPIVKGLPAGPGAAVGKVVFTAEDAVAAARRGE
                     SVILVREETNPEDVEGMRAAVGILTARGGMTSHAALVARGWGKCCIVGAGAMLVNSSE
                     KIAKISGTNIVIKEGDTLTLNGTRGHVYSNALKLMEATENPRFREFMKLVDKYRKMGV
                     RTNADTPEDAKIAREFGAEGIGLFRTEHMFYGKGSEEPLFLLRKMILSTTVEERRKAL
                     DELFIFVKRDIKNTLVAMDTLPVTFRLLDPPLHEFVPQSPEKQAELAKSLGITVEAVA
                     KRGENLHEVNPMMGHRGVRLGITYPEVTEMQVRAVFEATAELLKEGKSPHPEIMVPVT
                     CEVAELDFTRKIVDRVYAEVCQKFGMKSIEFKYGTMIEIPRACLLADEIARTTEFFSF
                     GTNDLTQMTFGFSRDDAGGFLPDYLDKKLLPADPFQTIDQKGVGQLIQMAVEKGRSTR
                     PELKVGICGEQGGDPASVEFCFKTGLTYVSCSPFRVPIARLAASQASIKYGK"
     gene            complement(9553..>9745)
                     /locus_tag="PK502_07900"
     CDS             complement(9553..>9745)
                     /locus_tag="PK502_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00778.1"
                     /translation="TATRHNSPRLRRGSNSAARAFPRYVPPPTHLRVRLLCRFSIPAT
                     YNDSRLDEQRGKYLLPRWG"
BASE COUNT         2409 a         2233 c         2302 g         2801 t
ORIGIN      
        1 tgcttgatgg ctacatgttc gatagcaaca tcgacagtgt tgctaccagg ttgggacgaa
       61 gcttttcctc tacagattcc cttaaggtaa ccgtggggac aggggttatt aaagggttta
      121 gccaggcgct tgtgctgatg aagaagggcg aatggggtag ggtaattatg ccctcttact
      181 gtggttatga ttctgtaggg agtaccacca taccgcctta ctctccccta attttcgatc
      241 tatacttcag cagtaagtct gtagtaacta aaccacagtc aaaatagcag ggcaaaaacc
      301 gtattaatct tgttgatatc agggatggga agctgtttcc actccctgat tttttttgtg
      361 gcaatatact ctccctccag ggtaaggcct gcagctatag ttagcctggc ctcatcggca
      421 caagtttgga gaatatccct gaagagagca acgttgcggt agggagcctc gataaaaagt
      481 tgggtttgat tttccgccct cgaacgttgc tccagctgcc ttattcgtcg ctttctctcc
      541 tcacttttaa cggggaggta gccaacaaag gcaaaatttt gcccgttcag cccgctggcc
      601 atcatggtca tgattatgga gctggggccg cacaatggaa caaccctaat tccatgatgg
      661 tgtgctgccg acaccagcat tgaacccgga tcggctattg ctggtaagcc cgattccgac
      721 atcagcccaa catcattccc atcaagcaag ggtttaagca tatcggttaa gtctgcagct
      781 gaggaatgct cgttaagagt gaagaaacaa agattttcaa tgggtgtttg aatgcctagt
      841 gctgaaaggt aacgacgagc tgtgcggagt tcttcaacta caaaaaattt caaccctctg
      901 gttagctcta ccactccgct tggaatcact tcgttccagc tagttgagcc aattgtcgtg
      961 ggtaccaggt aaagttttcc ggccatcctt tagtttttat ttactgcaac aagctatatt
     1021 tactgaaaat ttttgctaag gtagcgtttc gttgggatat tggtaagctg gattgaagta
     1081 acatagaaca tttcctgctg gttgatgttg tggttggata gttagcttgc cagctgagat
     1141 aatatgagct attctaactt ttttttacag tgaaaattac ttttttagga actggaaccg
     1201 cacagggtat tcccgtgata gcttgttcat gtccggtgtg tgcctcaacc gattcgcacg
     1261 acaagcgttt acgaacatcg gcgcttattg aggagcaagg tattaacatt ttacttgatg
     1321 ccggtcccga tttccggcaa caaatgctta acgccaacgt caaaaatctt aatgccattt
     1381 tgataacgca tgagcaccgc gatcatgtag ccgggctgga cgatgttagg gctttcaact
     1441 gggtgcagcg tcaacccatg gatatttggg ccgaagatag ggtgctggag gccattcaga
     1501 gggagtacgc ctatattttt gccgaacgaa aatatcctgg tattcccgaa gtggagctgc
     1561 accaagtgtt tgggcatcaa tttgacatac ttggagttac tgtaatcccc attcgcgttt
     1621 accattgcaa gctgccaata ttcggctttc gcataggcga tttgacctac attaccgatg
     1681 gcagcttcat ttcagaggag gaaaaggata aaattgtagg ctccagagtg ctggtgatta
     1741 atgctttaag gatgcaaaaa agcctttcgc atttttctct tcccgaagcc ttaaagctgg
     1801 tagaggagct tagtccgcga aaagcctaca ttacacatat aagccaccaa atgggattct
     1861 atcgggatgt tgcccggcag ctacccgaca atgttttcct ggcctacgat ggacttgttg
     1921 ttgaggttta agaaggttgt gcagaatttc ttcagttgat ttgcaatcag cttaaaagca
     1981 cacctcaggc caacaacttg tgtgctgttg tattataggg ttgtaagctc gccgatgatg
     2041 tgctgggcat tggcaaactt atcaacgata aaaagcacgt accggatatc aactgcaatg
     2101 gttcgttggg tctcgggctc aaacatgaag tcgcaggcca tggcttcgga gtttccatca
     2161 aacgccagac caataagatt cccattaccg tcgagtactg ggctaccgga gtttcctccg
     2221 gttatgtcgt tatcggtcag aaagcaaacc gggattgtac cgttacttgc ataacgtcca
     2281 taatccttgt tttgccaaag ctgacgtagt tttgggtcaa ccgtaaagac gtcctcattt
     2341 ggattttcct tctccatcac gccatcaagt gtggtgaagg gcttgtagta aagcccatca
     2401 gcaggcttgt agccggtaac cctgccatat gttaaccgca tggtggagtt tgcatccggg
     2461 taaagcagct tgcctgcatt catctccatc aaccctttgg tgtaaagctg catagccttc
     2521 tgaaaatcca ttctggcgtt gtgcatctga gggtaaattg aatcccatag cgcactatag
     2581 ttatggtaaa ggatgaaaag cgggtcggta agcagcgagt ccatgtaggg gtacttgatg
     2641 agcttctcca ggttttgagc cttcgtaaaa tatgactttt catagaaacc ttgtatgtag
     2701 tatgggtata ggctgtcgat gttgtcaacc ttttttatcg cattaagtag gcctaaattt
     2761 tggcaatcgc caatattggc tataaggtta ccaaatgctg ttgcgtacaa gcgcatatca
     2821 ggctcataga agtagctgtc aaaaatgccg ggaatatttt tcttgagtac cctggcttcc
     2881 cttctaatgc ggtcaacttt ccagaattta agtgggctgt agggtttgtt ttgctcaaaa
     2941 atggccccaa gcaggttgtt gcaggcattt acattcttgt aaacatcgac aggaaaaaag
     3001 gaaagggccg aaagcgagtt ttcaagcgct tcccatttgt tttgggtagc ctgttcaaaa
     3061 aagtttcggg taacaggaat ggtcggaagg tacttgcttc gaagcgaggg attttggtta
     3121 acccaggctt ttaggctatc ctcacgcgct gcaagccttt caactacatt gtatcggaac
     3181 attgactggg cttgccaaac atccttttga tagaagttga ccaggtagtc gtgcttgtcg
     3241 gtgtaggcca ccttggcctt ggggttgtaa cttctggcct gcttcacaat gctgatgaag
     3301 ttgccccaaa catcaacctt ccagggagct accacatcgc ggctgtactg agcttcgtag
     3361 gaagtggcat accggtaggt tgtgccgggg taccccataa tcatggcaaa atcgccttca
     3421 tttacaccct ttaggctgat ttttaggaag tttttaggtt tgtagggaac gtttcttggg
     3481 ctgtaggctg caggcttgcc atcgggtgca gtgtatattc tgaatatgca gaaatcacct
     3541 gtatgcctgg gccaatgcca gttatcaaca tcacccccaa agttaccaat tgagctaggt
     3601 ggaaccccaa ccaacctgac atccagaaac cgttcgtagg tgaacatgaa aaactggttg
     3661 tagttgtaga atggcttaac aatggtgtgg tagccggttt ttttcgatgt ttcatcctca
     3721 agctgctgca taacctccct cattttatca aaatactcct ggttggagag gcctgcatta
     3781 agcgaggcaa aaactttggc tgtaacatcc tcaaccccaa ccaaaataag ggcaacctgg
     3841 tcatgggcag gaagctcctc ggaaaggctg ccagcccagt agccattttc cagcaggtta
     3901 tttttttcgg agcttaaggc ctgaacggcc tccaccccgc agtggtggtt ggtaaagaac
     3961 attccctggg ccgaaaccag ctctcccgtg caacctccat cattaaactg aacaatggca
     4021 tccttcaggc tggagtggtt caggctgtat atatcctctg ctgtgagttt taagcccatt
     4081 gtttgcattg ttgcaatgtt ctggtggatt aagtttacca tccacatccc ctcatcggca
     4141 agggcactcc atacaccgaa acgggttatt acagctataa gcagcagctt tacaaggttt
     4201 cttttcataa ggattcaatt atttggtagc ctattacaaa tgctttagca tgttgcttta
     4261 tgggtagcca cactctaaaa cctatccatt tgttttgtta atttgtccat ttatggttgc
     4321 atcatcagag cacgttgatt ttgccattcg gtatttaggt tgcagctcat cagcaaaaca
     4381 ttcgaaatgt atgtttgccc ttgaaaacct actagggtag ggtgatcacg catacagcta
     4441 taagtctata tttttacagc tataatgata agctttttct atggcaaaaa tatcattttt
     4501 aaaatgattt tatggtaggg ctaatatttc aaccgtgcgt cgagaacgtc tccgtgtata
     4561 gtgtagcatt tttcatcgca ggcgctactt tccagcttag tcttaagcat agccccatca
     4621 actacgtata ttacccttcc cttgcaactg tatcttgcct taacagaata gaactggttg
     4681 atttctgcac gccagtagta ttcggctacg acggaggtat cggtgaatag cagctctccg
     4741 ctgtcggtat tccccctgta aacatcaaat atgacccagg gattttcgtc attgatggtt
     4801 agctttacgg taatgtcggt gcgggaaatt ttgtctttga agcattgcga gcagtcaaca
     4861 aaccagctgt tcagctcctc gcatgataag gggatagctg caagtagaac gatgaaaatc
     4921 aaccctttgt ttaatctacc tgacatattt tatcgttttg tttacaattc ttggcttggc
     4981 aatagggagg ttatacctga tattaatatt tgctctatga ggcgaggcat ccagtgtttt
     5041 taaccatgtg tagtcccatc caacggtaat ttcaaaagag tttatgtgca aattcagggc
     5101 tgcatttgct cccagcctta ccacatccgg agggttggta acggttccgc tggtatttcg
     5161 actaatccaa tcgaaagatg ggccaaactg ggctttcaac ccgatccctt tagggcgaaa
     5221 tacaattgga atgtagtgtc gcagctttac tgaaacaacc tgcttttcct cgtggagctg
     5281 gaataggttg ttgttttttg ggtaaagcac agcaacaggt gaaggacgaa accagtagcc
     5341 aactgatagt cccgttttgc ttatgctttc aactggttcg atttcaaaac cccaaagaaa
     5401 ttcatgattg ttaaatataa agcccgttcc aaccccaatg ctgcctatgg agggtttgag
     5461 cttagttttt aatccgtttt tcctaagaat ctctaaaacc tctctactcc ctgtttcggc
     5521 tgctagctgg tagtaactct ttgagagcgt atagctaccg gcattattct caaggagaag
     5581 ttgaatggtc tgtgaggaac gattagcaat ggcaagagca agtgcagtgg catttttttt
     5641 atcatgtagt ttgatatctg cgttgtagct aagcagtaat ttgcttaaaa gggtgtcgtt
     5701 aaactgagct gccaccatta gtggtgattt tccctcatta tcctgaatgt cagggtttgc
     5761 tcccgattcc agcagtaggg tggtgcagag gtttgtgcca ttgtaaaccg ctagcattag
     5821 cggagtgttt ccatggttgt cctgagcatt tacatcagcg ccatggtaaa ttaacaggtc
     5881 ggttaggtag ggaaatccat accaaacagc atagtgaaga ggggtaaggc ctttcctgtt
     5941 ggctacattt acctcggcgt tatagtcgag caggagttcg gcaatgctat cgttgcccgc
     6001 tttaaccgaa gcatgaaggg ctgtattgcc atcaaatggt acaaggttgg ggttggcgcc
     6061 atttttcagc agcagctcgg caatgtccct atacccccat tgcgaggcgt acatgagggg
     6121 tgttaccccc tgctcggttt gcgtgttggg gtcggcaccc agctccagca ggtactgaac
     6181 catgtcggta tgcccggagt cagcctcaaa tatcaacagc atgttgtagt gtgatttaaa
     6241 atctgccgta tctgttggca gtagctcatc atcctgtgcc cacaaccagg tggcagggag
     6301 aataagtagg aatagctgga taataactcg gattattttt gatttttgcc tgctcatggc
     6361 acaaaaataa aaaaaacgcc tccaaactgg aggcgttggt aggaaaaaac agtaaaacta
     6421 ttttccatac ttaatgcttg cctgcgaagc tgcaagacga gctataggaa ccctgaaggg
     6481 tgaacagcta acataggtaa ggccggtttt gaagcagaac tcaaccgaag ccggatcacc
     6541 tccctgttcg ccacagatac ccaccttaag ctcggggcga gtgctgcgac ccttttctac
     6601 cgccatttgt ataagttggc caacaccttt ttggtcaatg gtttggaacg gatcggcagg
     6661 caaaagtttt ttatccaggt aatcgggaag gaaaccaccc gcatcgtcgc ggctgaatcc
     6721 aaatgtcatt tgggttaagt cgtttgtccc aaaagagaag aactccgtgg tacgggcaat
     6781 ctcatcggcc agaaggcatg cacggggtat ttctatcatg gtgccatact tgaattcgat
     6841 ggatttcatt ccaaactttt ggcagacttc ggcgtaaaca cggtcaacaa tcttacgggt
     6901 gaagtcgagt tcggcaacct cgcaggttac tggaaccata atttcgggat gtggtgattt
     6961 gccttccttt agtagttcag cagtagcttc aaaaacagca cgcacctgca tttcggtaac
     7021 ttccggatag gtaattccca aacgaactcc cctatgtccc atcatggggt taacctcgtg
     7081 caggttttcg ccacgcttag ctacagcctc aacagtaatt ccaagcgatt ttgcaagttc
     7141 tgcctgtttt tccgggcttt ggggaacaaa ttcgtgaagg ggtgggtcaa gaaggcggaa
     7201 ggtaaccggt agggtatcca tagcaactag cgtgttttta atatcacgct ttacaaatat
     7261 aaagagctca tccagcgcct ttctacgttc ttcaacagtt gtgctaagaa tcattttcct
     7321 aagcaggaac aagggttcct cagaaccttt accgtagaac atgtgctcag ttctgaaaag
     7381 cccaattccc tcagcaccaa actcgcgggc aatcttagca tcctcaggtg tgtcggcatt
     7441 ggtgcgaacg cccatcttac gatacttgtc aaccaacttc ataaactcac ggaagcgtgg
     7501 gttctcggtt gcctccatta gcttaagtgc gttggagtat acgtgtccac gggttccatt
     7561 aagggtaagc gtgtcaccct ccttaataac gatgtttgta ccggaaatct ttgcaatttt
     7621 ttcggatgaa ttaaccagca tggcgcctgc tccaactatg cagcattttc cccaaccgcg
     7681 tgcaaccaga gcagcatgtg aggtcattcc gcctctggct gtaaggatgc caactgcagc
     7741 cctcatacct tcaacgtctt cggggttggt ttcctcgcga accaggataa cactctcgcc
     7801 tcttcttgct gcagcaacag catcttcagc ggtaaatacc accttcccaa cagctgctcc
     7861 aggacctgcc ggtagtccct ttacaatggg gttaactttt ttctcctctt ccgggtcgca
     7921 gatggggtgc agcagctcgt cgagctgggc tggttccacg cgtagcacaa cagttttttc
     7981 gtcaattaag ccctcgtgaa gcatatccat ggccatgttc agggcggctg tcccggtgcg
     8041 ctttcccgta cggcactgta gcatgtatag tttgccttcc tgaatggtga attcaatatc
     8101 ctgcatgtcc ttgaacgact tttcgagcgt gttgcggaca gccacaagct ccttgtaggt
     8161 atcgggcatt agctcctcca tgcttggaag gtgcttgttt tggtcgttct tggtttcgtt
     8221 gttcaacggg tttggtgtac ggatgccggc aaccacatct tcaccctgag cattgataag
     8281 ccattcgcca tagaatacat tttcaccggt tgcggggttg cgggtgaagg caacaccggt
     8341 tgctgagctg ttgcccatgt tcccaaatac catggcctga acgtttactg ctgttcccca
     8401 gtcgtctgga attccctcaa tcctgcggta cgaaacggcc ttcttaccgt tccagctctt
     8461 aaaaacagct ttaattccgc ccagcagctg ctccttggca tcatctggga attccttacc
     8521 caacgatttc tttacctgct ttttaaattc ttcagccaga tgttgaaggt cttctgcggt
     8581 gagttgggta tcgtccttgt aacccttgct attcttgtac tcatcaagta tatcgtcgag
     8641 aagctttcgt atgccctttc cgtcggtagg ctcaacacct tcagcctttt ccattaccac
     8701 atcagcgtac atcataataa gccggcggta agaatcccat acgaaccttg gattatttgt
     8761 ttttttaatc aatccaggga tggttgacga gcaaaggcct atatttagca ccgtgtccat
     8821 catcccgggc attgacttcc tggcacctga gcggcagctg acaagcaacg gattttcggg
     8881 atcgccaaac ttcattccca tgattttttc gatattcttc atggcctccc atatatcggg
     8941 aatgaggtca gagggtagct catggttatt ctggtagtac tcgttacaag tatctgtagt
     9001 tatggtaaac cctgcaggaa ctggaagccc tagtaggcac atttcggcca gatttgcccc
     9061 ctttcctccc agcagatttt tcatatctgc cttaccttca gcttttttgt taccaaaggt
     9121 ataaactctc ttaactttcg acatttatca atcaggtttt aattagactt ttagttatgt
     9181 ataagaagca caaattgaaa atcttagaca acttacaaaa gtattaaaaa attttggacg
     9241 tgattttctg tttccttttg agcgcaacat tttcttgcaa tcgtttgaat gggccggcta
     9301 tcgggtaggt gaatatgtaa gcttgcttat aagccgagag gttgagagca gctaaaaact
     9361 aaccctgaaa agttcgacag gtgttacagt gagcagttcg gcagactcac tgtaacacct
     9421 gttgagtgga gaatccgccg gtaggcggac aagttccgcc agcaggcgga caggttcttg
     9481 aatccgtcag gagccggaca agtttttgaa tcctgcttga atttttttat cctttaacct
     9541 ttaccctttt cttcagcccc aacgggggag aaggtatttc ccgcgttgct cgtcaaggcg
     9601 ggaatcgtta tacgttgctg gtattgaaaa acggcataaa agcctgaccc gcaaatgggt
     9661 cgggggcgga acgtagcgtg ggaatgcccg agcggcactg tttgagccac gccgcaggcg
     9721 gggcgagttg tgccgcgtgg cagtt
//
LOCUS       DAOPHX010000099         9650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_8767_length_9650_cov_5.032517, whole genome shotgun sequence.
ACCESSION   DAOPHX010000099 DAOPHX010000000
VERSION     DAOPHX010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9650
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8767_length_9650_cov_5.032517"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..681
                     /gene="rplC"
                     /locus_tag="PK502_07905"
     CDS             61..681
                     /gene="rplC"
                     /locus_tag="PK502_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPH00779.1"
                     /translation="MPQGLIGRKIGMTSVFVEDGKNVPCTVIEAGPCVVTQVKTVESD
                     GYTAVQLAFDEKKEKQTTAPMMGHFKKANTTPKRKLAEFRDWSGEMQLGDVITVDIFN
                     TDRWVDVTGITKGRGFQGVVKRHGFGGVGGQTHGQHNRQRHPGSMGASSYPSRVFPGK
                     RLAGRMGGDRVKIINLRVLKVIPESNLLLVKGSIPGAKGSYLIIEK"
     gene            681..1313
                     /gene="rplD"
                     /locus_tag="PK502_07910"
     CDS             681..1313
                     /gene="rplD"
                     /locus_tag="PK502_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPH00780.1"
                     /translation="MEAKVLSISGQDTGRTVQLDDSIFAIEPNDHAIYLDVKQILANK
                     RQGTHKSKERSEVSGSTRKLKRQKGTGTARAGSIKSPLFRGGGRVFGPVPRDYSFKLN
                     KKVKLLARKSALTYKVRENAFIVVEDFNIETPKTKEFITICKNLNVADKKTIFVLSET
                     NKNIYLSSRNLQQQKVVQASSLNTYEILHAKAMVLCESSVEVLHKMFKIS"
     gene            1326..1616
                     /gene="rplW"
                     /locus_tag="PK502_07915"
     CDS             1326..1616
                     /gene="rplW"
                     /locus_tag="PK502_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPH00781.1"
                     /translation="MDILIRPIVTEKMERLTGKLNQYGFIVDKRANKFQIKDAIQDMY
                     GVTVDRVNTMRYAGKRKSRYTRTGVVTGRTNSYKKAVVTLKAGDKIDFYSNI"
     gene            1622..2446
                     /gene="rplB"
                     /locus_tag="PK502_07920"
     CDS             1622..2446
                     /gene="rplB"
                     /locus_tag="PK502_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPH00782.1"
                     /translation="MAVRKLKPVTPGQRHKIIGTFEEITTSKPEKSLLRPLKKSGGRN
                     NQGKMTMRYIGGGHKRRYRLIDFQRSREDEPAVVKSVEYDPNRTARIALVEYPDGEKR
                     YIVAPNGIKVGQTVMSGHGVAPEVGNTLYLSEIPLGTLIHNIELYPGRGACMARSAGS
                     YAQLLAREGKYVVVKLPSGETRMVLATCKATIGTVSNPDHALEKSGKAGRSRWLGRRP
                     RNRGVAMNPVDHPMGGGEGKASGGHPRSRKGIPAKGYKTRSKKKASNKFIVERRKK"
     gene            2468..2737
                     /gene="rpsS"
                     /locus_tag="PK502_07925"
     CDS             2468..2737
                     /gene="rpsS"
                     /locus_tag="PK502_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPH00783.1"
                     /translation="MSRSLKKGPFIDYKLEKRVIEMNSTSKKSVIKTWSRSSMISPDF
                     VGHTIAVHNGNKFIPVYITENMVGHKLGEFAPTRTFRGHAGNRKK"
     gene            2765..3181
                     /gene="rplV"
                     /locus_tag="PK502_07930"
     CDS             2765..3181
                     /gene="rplV"
                     /locus_tag="PK502_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPH00784.1"
                     /translation="MGARKREMANRLKEERKDKAMAVLRNCPSSPRKMRLVVDQIRGK
                     DVNTALNILKFSPKHASKDVHKLLLSAIANWKSKNEGIRLEDANLFVKEIFVDQGRTL
                     KRIRTAPQGRAHRIRKRSNHVTVIVDSAPAINDTNN"
     gene            3189..3920
                     /gene="rpsC"
                     /locus_tag="PK502_07935"
     CDS             3189..3920
                     /gene="rpsC"
                     /locus_tag="PK502_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPH00785.1"
                     /translation="MGQKVNPISNRLGIIKGWDSNWYGGKNYSQKLVEDYKIREYLNE
                     RLSKASLSKIVIERTLKLITVTIHTARPGIIIGKGGQEVDKLKEELRKITNKEVQINI
                     FEVKRPDLDAVIVSNNIARQLEGRISFRRAVKTAIASTMRMGAEGIKVQVSGRLGGAE
                     MARSETYKEGRIPLHTFRADIDYALAEAHTKVGLIGIKVWICNGLVYGKRDLSPNLGI
                     SSAAPAQSGHGQARPHGARKKKSNK"
     gene            3943..4371
                     /gene="rplP"
                     /locus_tag="PK502_07940"
     CDS             3943..4371
                     /gene="rplP"
                     /locus_tag="PK502_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPH00786.1"
                     /translation="MLQPKKTKYRRQQKGRMKGNAQRGNQLAFGSFGIKALDSAWITS
                     RQIEAARQALTRHMKREGQIWIRIFPDKPITKKPAEVRMGKGKGAPEGFVAVVTPGRM
                     LFECEGVSLDIAKEALRLAAQKLPIATKFVVRNDYVEPVS"
     gene            4379..4579
                     /gene="rpmC"
                     /locus_tag="PK502_07945"
     CDS             4379..4579
                     /gene="rpmC"
                     /locus_tag="PK502_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPH00787.1"
                     /translation="MKASEVRELTDKEIVERIEAEKTNLLKLKLNHSISPLDNPMKIK
                     ESRRTIARLKTILTQRKLSQNQ"
     gene            4592..4855
                     /gene="rpsQ"
                     /locus_tag="PK502_07950"
     CDS             4592..4855
                     /gene="rpsQ"
                     /locus_tag="PK502_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPH00788.1"
                     /translation="MEEVKRNLRKVRTGVVVSNKMDKSIVVAVHRKVKHPTYGKYVKK
                     TTKFYAHDEANTCNIGDVVKIMETRPLSKLKRWRLVEIIERAK"
     gene            4858..5223
                     /gene="rplN"
                     /locus_tag="PK502_07955"
     CDS             4858..5223
                     /gene="rplN"
                     /locus_tag="PK502_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPH00789.1"
                     /translation="MIQQETRLKVADNSGAKEVLCIRVLGGTGMRYARIGDVIVVTVK
                     NAIPSGEIKKGTVSKAVVVRTKKEIRRPDGSYIRFDDNACVLLNAQGEIRGTRIFGPV
                     ARELRDNYMKIVSLAPEVL"
     gene            5246..5569
                     /gene="rplX"
                     /locus_tag="PK502_07960"
     CDS             5246..5569
                     /gene="rplX"
                     /locus_tag="PK502_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPH00790.1"
                     /translation="MASKLHIKKGDLVYVISGESKGKQGRVLSVQVSKQRAFVEGVNM
                     VSKHTKPNSKYPQGGIIKMEAPIHISNLMLIDPASGKPTRIGRRLNDKGKLVRYSKKS
                     GEEIK"
     gene            5569..6126
                     /gene="rplE"
                     /locus_tag="PK502_07965"
     CDS             5569..6126
                     /gene="rplE"
                     /locus_tag="PK502_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPH00791.1"
                     /translation="MEYVANFKRKYKEEIVPALMKEFGYKSVMQVPRLEKIVINQGVG
                     QAVSDRKIIEAAQADLTLIAGQKAVQTFSRKDISNFKLRKNVPIGLKVTLRRDRMYEF
                     MERLINVSLPRIRDFKGINDKLDGRGNYTLGIEEQIIFPEIDMDKINRLLGMEITFVT
                     STNNDEEAYALLREFGLPFKNAKKN"
     gene            6130..6399
                     /gene="rpsN"
                     /locus_tag="PK502_07970"
     CDS             6130..6399
                     /gene="rpsN"
                     /locus_tag="PK502_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HPH00792.1"
                     /translation="MAKESMKAREVKRAKLVEKYAEKRAKLKAEGDFIGLQKLPRNSN
                     PIRLHNRCMITGRPRGYMRQFGVSRITFREMASKGLIPGIKKASW"
     gene            6427..6822
                     /gene="rpsH"
                     /locus_tag="PK502_07975"
     CDS             6427..6822
                     /gene="rpsH"
                     /locus_tag="PK502_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPH00793.1"
                     /translation="MTDPIADYLTRIRNAVMANHRVVEIPASKMKLEMSRILFEKGYI
                     LNYKFEDDGKQGTIKIALKYHPETKKNAIKHIERVSRPGLRKYTNARELPRVLNGLGV
                     AIISTSQGIMTDKEARVKNIGGEVVCYVY"
     gene            6854..7408
                     /gene="rplF"
                     /locus_tag="PK502_07980"
     CDS             6854..7408
                     /gene="rplF"
                     /locus_tag="PK502_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPH00794.1"
                     /translation="MSRIGNKPVNLPQGVSVKISADNVVSVKGPLGELSQKVDPDITV
                     NLQGNLVVLTRPTDQKRHRSLHGLYRALINNMVIGVSTGWELQQELIGVGYKAEAKGQ
                     LLQLSLGFSHDIDFLLPNEVKVETKTERKGNPTITLKSIDKQLLGQVAAKIRSLRKPE
                     PYKGKGIRFVGEVVRRKAGKSASV"
     gene            7430..7789
                     /gene="rplR"
                     /locus_tag="PK502_07985"
     CDS             7430..7789
                     /gene="rplR"
                     /locus_tag="PK502_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPH00795.1"
                     /translation="MALSKEERRLRIRRRIRKKISGTAEKPRLTVFRSNTNIYVQFVD
                     DVTGRTLASASSMVKDIAGKPKITKTEQAKLVGQLAAKNALEKGITTVVFDRSGYLYH
                     GRVKALADAARESGLKF"
     gene            7795..8316
                     /gene="rpsE"
                     /locus_tag="PK502_07990"
     CDS             7795..8316
                     /gene="rpsE"
                     /locus_tag="PK502_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPH00796.1"
                     /translation="MSANTNIHRVKASDLELKDRLVSIQRVTKVTKGGRTFSFSAIVV
                     VGNEDGVVGYGLGKASEVTAAIAKGIEDAKKNLVKIPVFKGTIPHEQEAKYGGAKVII
                     RPATQGTGVKAGGAMRAVLESVGITDVLAKSKGSSNPHNLVKATVMALLELRDARTIA
                     EQRGISLSKVFNG"
     gene            8329..8502
                     /gene="rpmD"
                     /locus_tag="PK502_07995"
     CDS             8329..8502
                     /gene="rpmD"
                     /locus_tag="PK502_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009161000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPH00797.1"
                     /translation="MAKVKITQIKSVIGHPERQRRTLRSLGIKRMHQTVEVEGTPQVL
                     GMIAKVRHLIRVE"
     gene            8534..8980
                     /gene="rplO"
                     /locus_tag="PK502_08000"
     CDS             8534..8980
                     /gene="rplO"
                     /locus_tag="PK502_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPH00798.1"
                     /translation="MNLNNLKPAKGSTRNRKRVGRGTGSGHGRTSGRGQKGAQSRSGH
                     SYKVGFEGGQMPLQRRMPKYGFKNPFRIEYQAINIGTLQALAEKNGYSIIDVDTLIEA
                     GLVTRNERIKILAKGELTAKLEVKAHAFSKKAIEAIESANGTAVKI"
     gene            8985..>9650
                     /locus_tag="PK502_08005"
     CDS             8985..>9650
                     /locus_tag="PK502_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019967027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPH00799.1"
                     /translation="MKSFIQTLKNIFKIEDLRKRILYTLGILLVYRLGSFIVIPGLDP
                     DQLSALQEQTKEGIMGLLNMFSGGAFSNASIFALGIMPYISASIVVQLLGIAIPYFQR
                     LQKEGESGRNKLNQITRILTIAILILQAPAYLANLHAQLPESAFIVKGASFTIFATIV
                     LIAGTMFIMWLGEKITDKGIGNGISLIIMIGIIARLPFALVAEFFSRAEELGGGLVMF
                     LAEL"
BASE COUNT         2964 a         2059 c         2371 g         2256 t
ORIGIN      
        1 tccaagtgga gtcgaagtag aaattaaagt atgagtgtaa cctgtaaatt gtaaaaaaca
       61 atgccacaag gattaattgg acgtaaaatt ggaatgactt ccgttttcgt tgaggatggg
      121 aaaaatgtcc cctgcaccgt tattgaagcg ggtccttgcg ttgttactca ggtaaaaact
      181 gtcgaatctg atggttatac tgcggtgcaa ctagcttttg acgagaaaaa ggaaaagcag
      241 actactgccc caatgatggg gcattttaag aaggcaaaca ctacacccaa gcgtaagctc
      301 gccgagttca gggactggag cggcgaaatg cagctaggcg atgtgattac agttgatatc
      361 tttaataccg atagatgggt tgatgtaacc ggaattacca agggtagagg atttcaagga
      421 gtggtaaagc gtcatggatt tggtggtgtt ggtgggcaaa cccacggcca gcacaaccgg
      481 caacgccacc caggttcaat gggtgcttca tcctacccgt cacgtgtgtt tcctggcaag
      541 agactggcag gtagaatggg cggcgacagg gttaaaatta tcaacctacg cgtgcttaaa
      601 gtaatccctg aaagcaacct actcctcgtt aagggttcta ttccgggagc aaagggttct
      661 tacttaatta ttgagaagta atggaagcaa aagtacttag catatcagga caagatacgg
      721 gcagaactgt ccagctggac gattcaattt ttgccatcga gcctaacgat catgccattt
      781 acctggatgt gaagcagatt cttgccaata aacgacaggg aactcacaaa tcaaaagaac
      841 gtagcgaggt ttctggtagc accaggaagc tgaaacgtca aaagggaacc ggaactgccc
      901 gtgcaggtag catcaaatcg cccctattta gaggtggggg tagagttttt ggccccgtcc
      961 cacgcgacta cagctttaag ctgaacaaga aagtgaagct gcttgcccgg aaaagcgccc
     1021 taacctataa ggtaagggag aatgctttta tcgttgtgga ggactttaat atcgaaaccc
     1081 caaaaacaaa ggagttcatc actatttgta aaaacctcaa cgtagccgac aaaaaaacca
     1141 tattcgtgtt gagcgaaacg aataaaaata tatatttgtc gtccagaaat ttacaacagc
     1201 agaaggtcgt tcaagcctca tctttaaaca cttacgaaat actccatgca aaagccatgg
     1261 ttctttgcga aagttcagtt gaggtgctgc acaaaatgtt taaaatttca taaaccaagt
     1321 ggagtatgga catactaatt aggcctattg ttaccgaaaa gatggagcgt ttaaccggaa
     1381 agttaaacca gtacggcttt attgttgaca aaagggcaaa taaatttcag ataaaggacg
     1441 caatccagga tatgtatggc gttactgttg accgggtaaa tactatgcgc tatgccggta
     1501 aacggaagtc gcgttacacc cgtacaggtg tagttaccgg aagaaccaac agctacaaaa
     1561 aagcggtggt taccttaaag gctggagata aaattgattt ttatagcaac atttaaacga
     1621 aatggctgta cgcaaactaa aacctgttac accaggacag aggcacaaaa ttataggcac
     1681 attcgaggaa attaccacat cgaagcctga gaaatcgttg ctacgacccc tgaagaaatc
     1741 tggtggaaga aacaaccagg ggaaaatgac catgcgctac ataggtggtg gtcacaagcg
     1801 tagataccgc ttgatagatt ttcaacgcag tcgtgaagac gaacctgcag ttgtaaaatc
     1861 ggtggagtac gatccaaacc gcactgcccg catcgcgctg gtggagtatc cggatggcga
     1921 aaaacgctac attgttgccc ccaacggtat taaggtgggc caaacggtta tgtcgggtca
     1981 tggcgttgcc cctgaagttg ggaatacatt atacctgtcc gaaataccac ttggaacgct
     2041 catacacaac attgagctct accctggaag aggcgcctgc atggcccgaa gcgctggctc
     2101 atacgcccag ctccttgcca gagagggtaa gtacgtggtt gttaagctgc ctagtggcga
     2161 aacacgtatg gttctcgcaa cctgcaaagc taccattgga acggtttcca accccgatca
     2221 cgcactcgaa aaatcaggaa aagctggtcg ctccaggtgg ctcggacgtc gtccccgaaa
     2281 tcgtggggtg gctatgaacc ccgttgatca cccaatgggt ggtggtgagg gaaaagcctc
     2341 cggaggtcat ccccgctcac gcaagggaat ccccgccaag ggctataaaa cccgctctaa
     2401 gaagaaggca agcaacaagt ttattgtgga acgccggaaa aagtaatctg aataaaacaa
     2461 cgctattatg agtaggtcgc taaaaaaagg tcccttcata gattacaagc tggagaagcg
     2521 tgtcatcgag atgaacagca cttccaagaa atctgttatt aaaacctggt cgcgtagttc
     2581 catgatttct cccgactttg tgggacacac catagccgtt cacaatggta acaaatttat
     2641 tcccgtgtac atcaccgaaa acatggtagg acacaagctg ggtgaatttg caccaacacg
     2701 aaccttccgt ggacacgcag gtaataggaa aaagtaagtg aattcactaa attgatatta
     2761 agcaatgggt gcaagaaaac gtgaaatggc caataggctt aaagaagaga gaaaggacaa
     2821 ggcgatggct gttcttcgca actgcccgtc ctctccccgc aaaatgcgct tggtggtgga
     2881 tcagatacgt ggaaaagatg taaatacagc ccttaacata ctgaagttca gcccaaaaca
     2941 tgcctcaaag gatgtgcata agctgctcct ttcggcaatt gccaactgga agtcgaagaa
     3001 tgaaggcata cgcctggagg atgccaacct ctttgttaag gagatattcg ttgaccaggg
     3061 aagaaccctg aaaagaatta gaactgcacc acagggacgc gcacaccgta ttcgtaagcg
     3121 ctctaatcac gttaccgtga tagtagatag tgctccagca attaacgata ctaacaacta
     3181 acagctacat gggacagaaa gttaatccaa tatcaaacag gcttggaatc ataaagggtt
     3241 gggattcaaa ctggtatggg ggtaaaaact actctcaaaa gctagttgaa gactataaaa
     3301 ttcgcgaata cctaaacgag aggctctcca aggccagcct ttcaaaaatt gtaattgagc
     3361 gcaccctgaa gctgattacc gttaccattc acactgcccg tccgggaatc atcattggaa
     3421 aaggtggcca ggaggttgac aagttgaagg aggagcttcg taaaattacc aacaaggagg
     3481 tgcagatcaa catatttgag gtaaaacgcc ccgacctgga tgcagtgata gttagcaaca
     3541 atattgcacg gcagctcgag ggaagaattt ccttccgcag agccgttaaa accgctatag
     3601 catcaaccat gagaatggga gctgagggga taaaggttca agtatcgggt cggttgggtg
     3661 gagctgaaat ggcccgttcc gaaacctaca aggagggaag aataccccta catacctttc
     3721 gtgccgacat agattacgcg ttggccgaag cccacaccaa ggtaggctta attggtatca
     3781 aggtgtggat atgcaacggg ctggtttacg gtaaaagaga cctctcgcca aaccttggta
     3841 tttcatctgc cgcacctgcc caatcggggc atggacaagc caggcctcat ggtgctcgta
     3901 aaaagaaaag taataagtag aacaccttaa agcattctgg aaatgttaca accaaaaaag
     3961 accaagtata gaaggcagca aaagggtaga atgaagggca atgcccaacg gggaaaccag
     4021 ctggcattcg gttcattcgg gattaaggct ttggattcgg cttggattac cagccgacag
     4081 attgaagctg cccgtcaggc tcttacccgt cacatgaagc gtgagggaca aatatggatt
     4141 cgcatattcc ccgataaacc cataacaaaa aaaccggctg aagttcgaat gggaaaggga
     4201 aaaggagcac ctgagggttt tgtggctgtg gtaacccccg gccgcatgct tttcgagtgt
     4261 gagggggttt cgcttgatat tgccaaggaa gctctccgcc tggccgctca aaaacttccc
     4321 attgctacta aattcgtagt acggaatgat tatgttgaac ctgtaagcta gctagaacat
     4381 gaaagcatca gaagtaagag aacttaccga taaagagatt gttgaacgaa tcgaagcaga
     4441 aaagaccaac ctccttaagc tcaagttaaa ccatagcatt tcaccgctgg acaaccccat
     4501 gaagattaag gaatcgcgtc gtactattgc ccggttgaaa acaatcttaa cccaacggaa
     4561 gctaagtcag aaccagtaat gttgaattgc aatggaagaa gttaagcgaa atttacgaaa
     4621 ggtacgaaca ggtgttgtgg ttagcaataa gatggacaag tctattgtag ttgctgttca
     4681 ccgcaaagta aaacatccta catacggaaa gtacgtgaaa aaaaccacca aattttacgc
     4741 ccatgatgag gcaaatacct gcaacatagg cgatgtggtg aagataatgg aaacccgtcc
     4801 tctcagcaag ctgaaacgct ggagattagt tgaaataatt gaaagagcga agtagccatg
     4861 atacagcagg aaacacgatt aaaagtagcc gataacagtg gagccaaaga ggttttgtgc
     4921 attcgcgttc tcggtggtac cggcatgcgc tatgcccgga ttggcgatgt gattgtcgtt
     4981 accgtgaaaa atgccattcc ttctggcgaa attaagaagg gtaccgtttc aaaagcagta
     5041 gtcgttagaa ctaaaaagga aatacgcagg cccgacggct cctatatccg tttcgatgat
     5101 aacgcctgtg tgctgcttaa tgcacaaggc gaaattcgcg gaacccgcat atttggtccc
     5161 gtggccaggg agctccggga taactacatg aaaattgtat cactagcacc cgaggtgctg
     5221 taatccctta aaattcaaat tacatatggc aagcaagcta catataaaga aaggggacct
     5281 tgtttacgtc atttcagggg aaagcaaggg caagcagggc agagtgctta gcgttcaggt
     5341 aagcaaacag cgcgcctttg ttgaaggggt taacatggtg tccaagcata ccaagcccaa
     5401 ctccaagtac cctcagggtg gaattatcaa gatggaagcc ccaattcata tatccaacct
     5461 gatgctgatt gatcctgcat caggtaagcc tacccgcatt ggccgcaggc ttaacgataa
     5521 gggaaagctg gttagatact caaaaaaatc aggagaggag attaagtaat ggaatacgtt
     5581 gccaatttca aaaggaagta taaggaggaa attgttcccg ctttgatgaa ggagtttgga
     5641 tacaaaagcg tgatgcaggt tccccggctg gaaaagattg tcatcaacca gggcgttggt
     5701 caggctgttt ccgataggaa aattattgag gcagcccagg ccgatcttac ccttatagct
     5761 ggacaaaaag ctgtgcaaac tttttcgcgg aaggatattt caaactttaa gcttcgtaag
     5821 aacgttccta taggtcttaa agttaccctt cgtcgcgaca gaatgtacga gtttatggaa
     5881 cgtctgatca acgtttcgct tccccggata cgcgatttca agggtattaa cgataagctc
     5941 gacggacggg gtaactacac cctgggcatt gaggagcaaa taattttccc tgaaatcgac
     6001 atggataaaa ttaacaggtt gctgggcatg gagataactt ttgttacctc caccaacaat
     6061 gacgaggagg catatgccct tctgcgtgaa ttcggtttac cctttaaaaa tgctaaaaag
     6121 aactagtata tggcaaaaga gtcgatgaaa gcccgtgagg tgaagcgagc aaaacttgtt
     6181 gagaaatacg cagagaaacg tgcaaagctt aaagcagaag gcgattttat tggtcttcag
     6241 aagttacccc gtaactctaa tcctattcgc cttcataacc gctgcatgat tacgggcagg
     6301 ccccgcgggt acatgcgtca gtttggcgtt agcagaatca cattcaggga aatggcttca
     6361 aaagggctta ttcccggcat aaagaaagca agctggtaaa acacaacata attttagttt
     6421 acaatgatta ccgaccccat agcagattac ctcacccgaa ttcgtaacgc cgttatggcc
     6481 aaccatcggg tggttgaaat acccgcttca aaaatgaagc tggaaatgag ccgaattctt
     6541 ttcgaaaaag gctacatttt gaactataag tttgaggatg acggcaaaca gggtactatc
     6601 aaaattgccc tgaagtacca tcctgaaact aaaaagaatg ctattaagca cattgaaaga
     6661 gttagcagac caggattacg taagtacact aatgccagag aactaccccg ggtgctcaac
     6721 gggttaggtg tggccattat cagcacatcc cagggcatta tgaccgacaa ggaggcccgc
     6781 gttaaaaata ttgggggcga agtggtatgc tacgtttact aaataatttt actaaccaaa
     6841 tacaattttt ctaatgtcta ggatcggaaa taaacctgta aacctaccac agggtgtaag
     6901 cgttaaaatt agcgccgata atgtggtaag cgtaaaaggc cctctgggag agctctccca
     6961 gaaagttgac ccggacatca ccgtcaactt acagggaaac ctagttgttc tcaccagacc
     7021 aaccgatcaa aaacgccatc gatcccttca tggactttac cgagccctga ttaacaacat
     7081 ggtgattggt gtttcaacgg gttgggagct tcaacaggag ttgattggtg ttgggtacaa
     7141 ggccgaagcc aaagggcagc tgcttcagct gagcctcggc ttttcgcatg acatcgattt
     7201 cctccttcca aacgaggtga aggttgaaac caaaaccgaa cgtaagggaa accctacaat
     7261 caccctgaag agcattgaca agcagctgtt aggacaggta gctgccaaga tacgctctct
     7321 ccgtaaacct gaaccctata aaggaaaggg tattcgtttt gttggcgaag ttgttcgtag
     7381 aaaagccggt aagtcggcaa gtgtgtaacc tgttaatcta ttttgagata tggctttaag
     7441 taaagaggaa agaaggcttc gtataaggag acgaattcgg aagaaaattt cgggtacggc
     7501 tgaaaaacca cgattaacgg tttttaggag taataccaat atttatgttc agtttgtgga
     7561 tgacgttacc ggtcgaactt tagccagcgc atcgtcgatg gtaaaggata ttgctgggaa
     7621 acctaaaatt accaaaaccg aacaggccaa attggttggg cagctggctg caaagaatgc
     7681 cctggaaaag ggaattacca ccgtggtgtt tgaccgcagt ggctacctat accatggtag
     7741 ggttaaggca ttagccgatg cagctcggga atcaggactt aaattctaac gataatgtca
     7801 gcaaacacta acatacatag ggtaaaagcc agcgacctgg agttaaagga tcggctggta
     7861 agcatacagc gtgtaaccaa ggttaccaaa ggtggccgga ctttcagctt ttcagcaatt
     7921 gtagtagttg gcaacgagga tggcgttgtg ggttacggtt tgggtaaagc cagtgaagtt
     7981 actgccgcca ttgccaaggg tattgaagat gccaagaaaa acctggttaa aataccggtt
     8041 ttcaagggta ctattcccca cgagcaggaa gccaagtacg gtggtgccaa ggttatcatc
     8101 agacctgcca cccagggaac aggggttaag gcgggaggcg ccatgcgcgc cgtgctggag
     8161 agcgttggaa ttaccgatgt gctggcaaaa tctaaaggct catctaaccc tcacaacttg
     8221 gttaaagcta cggtaatggc acttctcgag cttcgtgatg ccagaactat tgccgagcaa
     8281 cggggtatct cattaagcaa agtttttaac ggttagtttt ttaaagccat ggcaaaagtt
     8341 aaaattaccc aaatcaagag cgttattggc cacccagaac gccaacggcg taccctcagg
     8401 tcgctgggca ttaaaagaat gcatcaaacc gttgaagtag aaggtactcc acaggtattg
     8461 ggcatgatag ccaaggtaag acaccttata cgggtggagt agtacctaca gaaccttaaa
     8521 aatggataaa actatgaatc taaataactt aaaaccggcc aagggatcta cccgaaatcg
     8581 gaaaagggtt ggacgtggaa cgggatccgg gcacggcaga accagcggac gcggtcaaaa
     8641 gggagcacaa tcgcgttcgg gccattccta caaggttggt ttcgagggtg gccagatgcc
     8701 cctgcagcga cgaatgccta aatatggttt taaaaatcca ttcaggattg aataccaggc
     8761 cattaacatc gggacactac aggcgctggc tgagaaaaat ggatattcaa ttattgacgt
     8821 ggataccctg attgaagcag ggttggttac ccgtaacgag cgtatcaaaa ttcttgctaa
     8881 gggcgaactt actgcaaagc tagaggttaa agcccacgca ttttctaaaa aagcaatcga
     8941 ggctatcgaa tcagccaacg gaacggctgt taaaatttaa atggatgaaa tcctttattc
     9001 aaacacttaa gaatattttc aagattgagg accttcgaaa gaggatcctc tatactcttg
     9061 gaattctctt ggtttacagg cttggtagtt ttattgtaat tccagggtta gaccctgacc
     9121 agctttctgc tctgcaggaa caaaccaagg aaggaatcat ggggcttctg aacatgttct
     9181 cgggtggtgc tttttctaat gcatccatat ttgccctggg tattatgccc tacatttcag
     9241 cctccattgt ggtgcagcta cttggaattg caatacccta ctttcaacgg ttacagaagg
     9301 agggagaaag cggtcgtaac aagctaaacc aaattacccg aattcttacc attgctattc
     9361 ttattcttca ggctcctgcc tacctggcaa accttcatgc ccagcttcct gagtctgcct
     9421 ttattgttaa aggtgctagc tttaccatat ttgctaccat tgtgctgatt gccggaacca
     9481 tgtttatcat gtggcttggc gaaaagatta ccgataaggg tattggcaac gggatttcac
     9541 ttataatcat gattgggatt attgcccgac ttcctttcgc actggttgct gagttcttct
     9601 caagggccga ggagcttggt ggaggtttgg ttatgttcct tgccgagctg
//
LOCUS       DAOPHX010000100         9328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_9209_length_9328_cov_5.573062, whole genome shotgun sequence.
ACCESSION   DAOPHX010000100 DAOPHX010000000
VERSION     DAOPHX010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9209_length_9328_cov_5.573062"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PK502_08010"
     CDS             <1..1235
                     /locus_tag="PK502_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPH00800.1"
                     /translation="ENYIARRFFKGTNGMSLSRPFIRITITSVALSIAVMIVAVAVIT
                     GFKQEIKAKVAGFGSHIQITNYDSNQSLETLPLPSNLKCVEDLKAIKGVRHIQVFGIK
                     AGIIKTPSDMQGVALKGVSSDFDWSFFRQNLVEGSIISVSDSTPTNQTLISKTLANLL
                     RLKVGDSYDVFFVQEPPRFRRFVVEGIYDTKMVEFDKLFVICDIGHIRRLNGWSSNQV
                     SGYEVLVDNIEKVDDIAIRVDDAVGYDFLDDGSRLKVQSLTERYSSIFDWLELQNINA
                     AVLIILMLLVAGINMISGLLIIILDKTGTIGLLKALGTNNESIRKIFLLQSAYIMIRG
                     LAIGTIAGIAICLLQKYLGIVKLDEEYYFLSVVPINMVWWHIVLLNLASFIVGLAMMV
                     IPSMVVSRISPDKTLKFD"
     gene            complement(1672..2442)
                     /locus_tag="PK502_08015"
     CDS             complement(1672..2442)
                     /locus_tag="PK502_08015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPH00801.1"
                     /translation="MKNEKHTIHEFDLNIIYDFFSNTARQGPGSPEETLKALSFVDGL
                     TEKSKIADIGCGTGGQSMVLGENTPCEIIGVDVWPDFIKQFNQNAHIKNLQKRVKGIV
                     GNMESLPFKEEELDLIWSEGAIYNIGFERGLSEWRKFLKPGGYIAVTENTWFSEVRPA
                     EIQDFWQKAYPEIDTIPNKVAQMQKAGYLPIATYILPETIWTDYYASQNLRLEPFLKK
                     HNGNKMVEEFIASQRYEAELFYKYKAYYGYMFYIGKKI"
     gene            3400..4419
                     /locus_tag="PK502_08020"
     CDS             3400..4419
                     /locus_tag="PK502_08020"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPH00802.1"
                     /translation="MQNLDWKNLPFGYLKTDYNVRCRYSDGKWGNLEVSDSEYINIHM
                     SATALHYGQEGFEGMKAYRGKDGRIRIFRWEENAKRLQLTADGILMAPVPMELFHEAV
                     VKAVKLNEKYVPPYGTGASLYIRPLLFGSGAEIGVKPAKEYVFIVFVTPVGPYFKEGF
                     NPTKIAIIRDSDRAAPLGTGTFKIGGNYAASLRGTIKAHNAGYSSPMYLDACGKRYID
                     EIGAANFFAIKNNTYITPKSTSILNSITNMSVMQLAQDLGLKVERRPVAVEELETFEE
                     VGACGTAAVISPIGEINDIDGGKVYKFCKDGKAGPISTKLYQTLTGIQYGDIEDKHGW
                     VSFVE"
     gene            complement(4537..6132)
                     /locus_tag="PK502_08025"
     CDS             complement(4537..6132)
                     /locus_tag="PK502_08025"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPH00803.1"
                     /translation="MAPLFSAKNQLARKANRLFRAIAYRVALLILFAGIGINGHADEA
                     DTLLTKPITISLPAENLYNALNHIGERAGCFFIYDSRDVRGSKRIKPVTWVNKPMYEL
                     LNLLVADSLVSYRVIDRHILIYRKSKDAHSGDSTRRESYLQVGGRLYDGETRQVITFA
                     TVTLNEIGLGTITNNDGAFTMRIPSSFIHASVVISHLGYQSQTIPIELLLGKKYTIYL
                     QPRYIPIQEVIIRNIDARGIVTAMVENRYKNYASNDVYLTGFYREGVSRNNQYLGYSE
                     AIFRIYKSAITESTSSDQVKLLKARKLEKQDRSDTLTVKLMAGIKGTLEMDIAKSLPD
                     FIDPEYMDSYTYSKSDIVAHDSRTAYAISFEQKKGVELPLYMGTLYVDVENLALLAAD
                     FELNPKYIEKAASSLIVRKNRSVKVKPQQVSYSVSYRELNGVYYVQHIRGDISVRISS
                     RYRLFTDSYNLFFEFVNMQIDTLNVQRFSRRDVVKPYTVFSDGDFAYDASFWGDMNFI
                     EPEEDVARAIARINPKIESVVGE"
     gene            complement(6092..7099)
                     /locus_tag="PK502_08030"
     CDS             complement(6092..7099)
                     /locus_tag="PK502_08030"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPH00804.1"
                     /translation="MINSTPNTQDYKKISRYLCDDMLFPERLIFLALVACKPRLRKNF
                     KAARMDMAMADKLISAGSYNPNVAWEKLHDRLENESLIPDEIRNTVTFYKGAYLKYAA
                     IAALAILLIGIPGYFLFSPSRIVVENGRDGSTMITSLPDGTSVYLAENSTLSYPKHFK
                     GKLRSVTLNGEAFFEVAPNPHKPFIVNTKAATVRVLGTTFNIKSTDSNNFELCVVEGK
                     VKVSSLGGDVLATAGERVYVRNSLLQKSISAGQPTVKHRMTRLQFQDERMEDIVGIIN
                     RTYGSNIELMGNNLRNRRISVTFENELSSIVSILSASFNLQIETEPDGTIVLSEKSVS
                     P"
     gene            complement(7148..7693)
                     /locus_tag="PK502_08035"
     CDS             complement(7148..7693)
                     /locus_tag="PK502_08035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HPH00805.1"
                     /translation="MSLNGLNRQSRIKDDSIGDFEVLFKTYYVPLCRFAFSIVRDSDQ
                     AEEIVQEFFYNYWKNRKNIGIKFSIRAYMYKAVKNSALRYLAHRKVESSYGVTLGSTS
                     GGFDNLDESSDELLKIVEATLQQLPERCRQAFILSRRDGLKYKEIAAKLSVSEKTVEA
                     DMTKALMALRQNLSRLGFNTH"
     gene            complement(7780..9051)
                     /locus_tag="PK502_08040"
     CDS             complement(7780..9051)
                     /locus_tag="PK502_08040"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPH00806.1"
                     /translation="MMKVLAGFFRKSLKYSAMVIALLISASVWAQEKDYITVNGVVKD
                     SKTKERLVFSGIYVPGTSIGTVANSEGQFTLKIPKTLNVQEFGISHLGYHNGIFPVSD
                     GNGRRENVYYLDPASVEIKGVVVRPKDPRTLILQALNRIEDNYSRVPNTLTGFYRETI
                     KQRRDYVSIAEAVTEIYKSPYKFTLESDRIKVIKGRKGDNVKKADTLIVKMQGGPNVS
                     LYLDIVRYPELILGLEEMKHYQYELTDMVSIDNRSNYVISFKPLAKLEYPLYYGKIYV
                     DVESLAITMAEFSMDISDVEKATQAFVRKKPAGLIFEPVSTSYLVTYKKLGDRYYLNY
                     MRSEIKFQADWKKKIFKTSYTLMSEMAITERDTVNAAKIAAKESFKPYAILTDKVNEY
                     FDEDYWGSYNTIEPDESIQSAIAKYNRRFKR"
BASE COUNT         2510 a         2115 c         2037 g         2666 t
ORIGIN      
        1 ccgaaaacta catagcccga cgattcttta aaggcacaaa cggcatgagc ctgtccaggc
       61 cttttatccg tataaccatt acctctgttg cccttagcat tgcagtgatg atagtagcag
      121 tagccgtaat aaccgggttc aagcaggaga taaaggccaa agtagccgga tttggtagcc
      181 atatccagat aaccaactac gacagcaacc agtcgcttga gaccctcccg ttaccatcga
      241 atctaaagtg cgtggaggac cttaaagcga ttaagggggt taggcatatc caggtttttg
      301 gtattaaggc cggtattatt aaaacaccct ccgatatgca gggagtggcg cttaaaggag
      361 tttcctccga ttttgactgg tcgtttttcc gtcaaaacct ggtggagggc agcattatta
      421 gcgttagcga ctccaccccc accaaccaaa ccctcatatc caaaacactg gccaacctat
      481 taaggctaaa ggttggcgac tcctacgacg ttttctttgt tcaggaaccc ccacgcttcc
      541 gacgtttcgt agtagaggga atctacgata ccaaaatggt ggagttcgac aagctatttg
      601 tcatctgcga cattgggcat atccgccgtc ttaacggatg gagcagcaac caggtaagcg
      661 gttacgaggt gctggttgac aacattgaaa aggtggatga catagccatc cgggttgacg
      721 atgccgtggg atacgatttt ctcgacgatg ggtcacgatt aaaggtacaa agcctgaccg
      781 aacgctactc cagcatattt gactggctgg aactgcaaaa cataaatgca gcagtgctca
      841 tcatactcat gcttctggtt gccggcatca acatgatttc gggtcttctc atcatcatcc
      901 tcgacaaaac gggaactatc ggcctgctaa aagcgctcgg aacaaacaac gagagcattc
      961 gcaaaatttt cctcctacag tcggcctaca tcatgattcg cggtttggct ataggcacaa
     1021 ttgccggaat tgccatttgt ttgctacaaa agtatttagg aattgttaag ctcgacgagg
     1081 agtactactt cctgtcggta gtgcccatca acatggtgtg gtggcacatc gttttactca
     1141 atctggcttc atttattgtt gggcttgcca tgatggttat accctccatg gtggtttcgc
     1201 gtataagccc cgacaaaacg cttaagttcg actagcatgt cgtaaccatt acaaaggtgg
     1261 cctaacccgc ataatcccct cttaaccgaa gattggaaaa tgttgttggc ggaataggct
     1321 gggcggccgg ctctgtcgtt tttcgggtaa tactactgga atagcttaca gagcatgatg
     1381 agagaaaaat gccagcctaa ccagcggccc accaaacgct cagggtcacg cccacagcca
     1441 actgtaaaaa tacccgtaga gcatgcacct ttactaacag caaagaaata cagccttttt
     1501 caggagtaac catggagaaa agtctaggtc aggggacttt ggcgggtaga aacatttacc
     1561 aatttacatt tcccctagtt gttaaaaaac ctttcgtgcg taaaagttct cgcacgagag
     1621 gttttttcaa ttttttaaat caagtcaaaa atttatggtt agctccattt tctaaatttt
     1681 cttaccaata taaaacatat agccgtagta tgctttgtac ttgtaaaata gctcagcttc
     1741 gtaacgctgg gaggctataa actcctctac cattttattg ccgttatgtt tttttagaaa
     1801 gggctctaac ctcaagttct gggaggcata gtaatctgtc caaatagttt cgggcaagat
     1861 atatgtggca ataggaagat agcctgcttt ttgcatttga gcaaccttat tgggaatagt
     1921 atcaatttcg ggataggctt tttgccaaaa atcctgaatt tcggcaggac gcacctcgct
     1981 aaaccaggta ttttcagtaa cagcaatata tcctcccggt ttcagaaatt tacgccattc
     2041 gctaagtcca cgctcaaatc caatgttata tattgcgcct tccgaccaaa tcaagtctaa
     2101 ctcttcttct ttaaaaggaa gactttccat gttgccaaca atgcctttca ctcttttctg
     2161 aagatttttg atatgggcat tttggttgaa ttgttttata aaatcgggcc aaacatcaac
     2221 accaataatc tcgcacggtg tgttttctcc caaaaccata gattggccac ctgttccaca
     2281 accaatatcg gcaattttgg acttttcggt aagaccgtca acgaagctca atgctttaag
     2341 cgtttcttca ggacttcctg gaccttgacg tgctgtattt gagaaaaaat cataaataat
     2401 gttcaggtca aattcatgaa ttgtatgttt ttcgttcttc attttggctt attttgttgc
     2461 atacacgaat agcaacgcat tgctaatatc attgtacgcg cgttaacact ttagttaatt
     2521 taatagaaaa ctgctcccga agaggattat ccgagaacaa acaaggatac aatctataca
     2581 aaaagtacag atcgtttaca ggacaatatc cgagttaacc ataaacatcc agttgcttta
     2641 acataggcaa aagtattaaa atttaaaagt tatgaagctc cgttcaaatc atttttactg
     2701 caagagttgt cttagaaaac gtttttcact tgtaaatagc tggctcccgc ctgattggct
     2761 tagacgaact gtcggatcgg ttcatgtcgt gtggcaaccc aatccacacc gctcaaccag
     2821 ggcgctcaac actcttctta ctaaatttaa aacccatagt ccttccggat ttgcttttta
     2881 atagctgcta cagcgttctt tcattctctc cactaaaact tacgcactac tcaacaccat
     2941 aggggatgac aaatttcgtt gggctctccg ccggctggcg aactggttcg tgtggtagca
     3001 tggggtgtgg tagcctattg ttttgctcaa cgtggcttcc ttcattgttg ggccagccaa
     3061 gaaggccgtc ccctccatgg tggtctcaag agtaaacact aaaatgcgcc acgctcgtat
     3121 aaaaaaatca tcgccactac gaggcgtttt aaaaaatcct tacgagtatt gccaacccgg
     3181 tcaatctccg ccatacagat aaaaactagc atcagctcaa aatgtcgtat gcactgttac
     3241 catcaccgtg tagttacaat ttttaattca tattgaatat tttattaaac ttttaaatca
     3301 tttagctgtc ataatagcac taaatgttag gagaaataat ataattctac taattttgtg
     3361 aggcaacatt gttaataaca attctaaaaa tctgcagtca tgcaaaatct tgattggaaa
     3421 aaccttccgt ttggttattt gaaaaccgat tacaacgtaa ggtgtcgtta tagcgacggt
     3481 aaatggggta atctggaggt ttcggattcg gagtatatca acattcacat gtcggctacc
     3541 gccctgcact acgggcagga aggctttgag ggcatgaagg cctatcgtgg taaagacggt
     3601 cggattcgca ttttccgttg ggaggaaaac gccaagcggc ttcagctaac agctgatggc
     3661 atactgatgg cacccgtgcc gatggagctt tttcacgagg cggtagtaaa agccgttaag
     3721 ctaaacgaga agtatgttcc accctacgga acaggagcat cgctatacat caggccgctg
     3781 ctgttcgggt cgggtgccga aattggcgtt aaaccggcaa aggagtacgt ttttattgta
     3841 ttcgttacgc ccgtaggccc ctacttcaag gagggcttta acccaacaaa aatagccatt
     3901 atacgtgata gcgatagagc cgctccgctg ggaaccggca cgtttaaaat tggtggtaac
     3961 tatgcagcca gccttcgtgg caccataaaa gcgcacaatg caggctacag ctccccaatg
     4021 taccttgatg cctgcggaaa gaggtatata gatgaaattg gtgctgcaaa cttcttcgcc
     4081 atcaagaaca acacttacat tactccaaaa tcaacctcta tactcaactc cattaccaac
     4141 atgagcgtaa tgcagctggc acaggattta ggcctgaagg ttgagcgccg tccggttgcc
     4201 gttgaggaac ttgagacttt tgaggaggtc ggagcatgcg gcactgctgc agttatttcg
     4261 ccaattggcg aaataaacga tattgacggc ggaaaggttt ataaattctg taaagatgga
     4321 aaggcagggc caatatcaac caagctatac cagaccctta caggaattca gtacggtgat
     4381 attgaagata agcatggatg ggttagcttt gttgaataag tagtattaaa gtaacgcaca
     4441 aaaacccttg ggggtcttct cccgagggtt ttttatcggg ttataccttt atagggttaa
     4501 aataccatgc aggtaattgc aaaccgaaga ggctagctac tcccctacga cactctcaat
     4561 tttagggttg attcgggcaa ttgcccgggc cacatcctcc tcgggctcaa tgaagttcat
     4621 atctccccaa aaactcgcat cgtaggcaaa atccccatcg gagaaaacgg tgtagggttt
     4681 tacaacatct cgtcttgaaa agcgctgaac gttaagggtg tcaatctgca tgttcacgaa
     4741 ttcgaaaaac aggttgtagc tgtcggtaaa tagcctgtag cgtgagctaa tgcgtacgga
     4801 aatatcaccc cggatatgct gcacgtagta aactccgtta agctccctgt aggacacaga
     4861 gtagctaacc tgctggggtt ttacctttac ggagcgattt ttcctaacaa taaggctgct
     4921 ggctgccttt tcaatatact tcgggtttag ctcgaagtcg gcagccagga gcgcaaggtt
     4981 ttccacatca acgtaaaggg ttcccatgta aagagggagt tcaacacctt tcttttgctc
     5041 aaatgatatg gcataggccg ttctgctatc gtgtgctaca atgtccgact tggagtaggt
     5101 gtagctatcc atgtactccg ggtcaataaa atcgggtagg cttttagcaa tgtccatttc
     5161 gagagttccc ttaatgccag ccatcaactt aacggtcagg gtatcgcttc tatcttgctt
     5221 ttcgagttta cgcgccttta gcagcttgac ctgatcgctg gatgtgcttt cggtaatggc
     5281 cgatttgtaa atcctaaata ttgcctcgga atagccgagg tactggttat tgcggctaac
     5341 cccttccctg taaaatccgg taaggtaaac atcgtttgag gcatagttct tgtaccggtt
     5401 ttcaaccatg gccgtcacaa ttccacgagc atcaatattc ctgataatta cctcctgaat
     5461 ggggatgtat cggggttgaa ggtaaatggt atatttcttc ccaagcagca gctcaatagg
     5521 aatggtctgg ctctggtagc ctaaatgtga tatgaccacg ctggcatgaa taaacgagga
     5581 gggaattctc atggtgaaag ccccatcgtt gttggttatg gttccaagtc caatttcgtt
     5641 tagcgtaaca gttgcaaacg taattacctg gcgggtttcc ccgtcgtaca gccttccacc
     5701 cacctgcagg tagctttcgc gccgggttga atcgccggaa tgagcgtctt ttgatttcct
     5761 gtaaatcaga atatgcctgt caattaccct gtagcttacc agcgaatcgg caaccaggag
     5821 gttcagcagc tcatacatag gtttgtttac ccaggttacc ggcttgatac gcttacttcc
     5881 ccgcacatcg cggctgtcgt aaatgaaaaa acaacctgcc ctttcgccaa tgtggttcag
     5941 ggcattgtaa agattttcgg ccggaagcga tatggttatt ggcttggtca gcagcgtatc
     6001 ggcttcatca gcatggccgt tgatcccgat acctgcaaaa aggattagaa gcgcaactcg
     6061 atatgcgatt gccctgaaca gcctgtttgc cttacgggct aactgatttt tcgctgagaa
     6121 caatggtgcc atcgggttct gtttctattt gcaggttaaa ggaggccgaa agaatggaga
     6181 caatgctgct aagctcattt tcaaatgtaa cagaaattct cctatttctc agattgttgc
     6241 ccattaactc aatgtttgac ccgtatgttc tattgattat tcctacaata tcctccatcc
     6301 tttcatcctg aaactgaagt cgtgtcattc tatgctttac ggttggctgt cctgcagaga
     6361 tggatttttg aagcaagcta ttcctcacat aaacccgttc accggctgta gccagcacgt
     6421 caccgcccaa gctgctcact tttacctttc cctccaccac acaaagttca aaattattgc
     6481 tatcagttga tttgatgttg aaggtggttc ccaaaaccct gacagtggca gcctttgtgt
     6541 tcacaataaa gggtttatgg gggtttggag caacctcaaa aaatgcctca ccattaaggg
     6601 taacgcttcg taatttgcct ttaaagtgct tggggtagct cagggtggag ttttcggcca
     6661 ggtaaacgct agttccatcg ggaaggctgg taatcattgt gcttccatct ctcccatttt
     6721 caaccacaat tcttgagggc gaaaaaagaa agtagccggg tattccaatg agtaatatgg
     6781 ccaaagcagc aatggctgca tatttcaagt atgctccctt gtagaaagtc actgtattcc
     6841 ttatttcatc agggataagg ctttcgtttt ccaacctatc gtgcaatttt tcccacgcca
     6901 cgttggggtt atatgatcct gcactaataa gcttatccgc catcgccatg tccatccttg
     6961 cagccttaaa attcttcctt aaccgaggct tgcaggctac cagagcaagg aaaatcaacc
     7021 tctcgggaaa aagcatgtcg tcgcataggt agcgggatat ttttttgtaa tcctgggtat
     7081 ttggtgtgct attgatcatc gttatctata aaaataaaaa tagttgtttt gaaaaccttt
     7141 gcctccttta atgggtatta aaacccaatc ttgacaggtt ctgcctgaga gccatcagcg
     7201 cttttgtcat atcagcctca accgtttttt ccgaaaccga aagttttgct gcaatttcct
     7261 tgtatttaag cccatcccta cggctaagta taaatgcctg acggcatctt tcgggcagct
     7321 gttgaagtgt ggcctcaaca atttttaaca gctcgtcgga gctctcatca agattatcaa
     7381 aacctccaga tgtactacct aaggtaacac catagctgct ctccaccttt ctatgtgcca
     7441 ggtatcgcaa tgcgctattc ttaacagctt tgtacatata tgccctgatt gaaaacttga
     7501 tgccgatatt tttacggttt ttccagtagt tgtaaaaaaa ttcctgaaca atttcttcgg
     7561 cttgatcgga atctctgacg attgaaaagg caaaccggca aagcgggaca tagtaggttt
     7621 tgaagagaac ctcaaaatca ccaatgctat catccttaat cctgctttgc cggttcaacc
     7681 cgttcaggct catctataat caggctacgg tattagtcca aaggtataaa ataattggct
     7741 acgggcagaa acaataggta ggctaagatg cccatagggt taccttttaa acctacggtt
     7801 gtacttggca atagccgatt gaatggattc atcaggctca atggtgttat atgagcccca
     7861 gtagtcctcg tcaaagtact cgtttacctt gtcggttaaa atggcataag gtttaaacga
     7921 ttcctttgca gcaatcttgg cagcattaac ggtgtcacgt tcggtaatgg ccatttcgga
     7981 catgagggtg tagctggttt taaaaatctt ctttttccag tcggcttgaa atttaatttc
     8041 gctgcgcatg tagtttaggt agtacctgtc gcccaacttt ttgtaggtaa ccaggtagct
     8101 ggtgctgacg ggttcgaaga tcagcccggc aggttttttc ctgacaaatg cctgggtagc
     8161 cttttcaaca tccgaaatat ccatgctaaa ctcggccatg gtgatggcca gcgactccac
     8221 gtcaacgtat atcttaccgt agtaaagcgg gtattcgagc ttagcaagtg gcttaaatga
     8281 aataacatag ttggaacggt tgtcgatgct gaccatatcg gtaagctcgt attggtagtg
     8341 cttcatttcc tccaacccca gtataagctc ggggtacctg actatatcca gataaaggga
     8401 aacattgggg ccaccctgca ttttcacgat aagggtatcg gcctttttca cgttatcgcc
     8461 tttacgcccc ttgataacct taatcctatc cgattccagg gtaaacttat agggtgattt
     8521 gtagatttcg gtaacagctt cggcaatgga cacatagtcg cgacgctgct taatggtttc
     8581 gcgatagaaa ccggtcaggg tattgggaac cctgctgtag ttgtcctcta tccggttgag
     8641 ggcctgcaag attaaagtcc gtgggtcttt gggccgaaca actacaccct taatctcaac
     8701 cgaggcggga tcaaggtagt acacattttc acgtcgcccg ttcccatcgg aaaccgggaa
     8761 aataccatta tggtagccca ggtgggaaat tccaaattcc tggacattca acgttttagg
     8821 aattttcagg gtaaactgcc cttcggagtt ggccactgtt ccgatgctgg ttccgggaac
     8881 atatattccg gaaaacacca atcgctcctt cgtttttgaa tcctttacca caccgttaac
     8941 ggtgatgtag tctttttcct gtgcccaaac gcttgctgaa ataagaagtg caataaccat
     9001 tgcgctgtat ttcagacttt ttctgaagaa tcctgctagc actttcatca ttcttgctgg
     9061 tttatagggt tttaacttca atgatttttt gttcgatcag cttacattaa tcaagtaaaa
     9121 ttaatccttt gcatatatga tacccggaaa ggcatttacc ctataaaaaa tggattatta
     9181 tataaatttt aattcaatgt cgtttagtta aggtcaagat tcaagaactt gtccgcctct
     9241 tggcggaact tgtccgtctc ctgacggatt cacccttcga caggtgttac agtgagtagt
     9301 tcgtatggtg ttatagtgag ccagtcga
//
LOCUS       DAOPHX010000101         9316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_9231_length_9316_cov_5.551668, whole genome shotgun sequence.
ACCESSION   DAOPHX010000101 DAOPHX010000000
VERSION     DAOPHX010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 9316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9316
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9231_length_9316_cov_5.551668"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..797)
                     /locus_tag="PK502_08045"
     CDS             complement(69..797)
                     /locus_tag="PK502_08045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPH00807.1"
                     /translation="MGNRFIRILLLLLISSHSIAQTSFNELVSSANLNLKEKNYKAAA
                     DQFKKALEIQPDDTVALQGVIKCYTLSEDYKNAQRYADIALGKFPTYPEFHLLQGIIY
                     NLKGNHEEAISAFSHALELKPSNRTSVQILMNRASAEIRLDNFTDALADYNAAMELDP
                     RNTSIYTYRGLVNFRLGFYIDAVNDYTNAIDLEPNSALTYYNRGMTYLKLSEKQKACA
                     DFHMACKLGDMNACKMILTECGGR"
     gene            complement(892..1516)
                     /gene="recR"
                     /locus_tag="PK502_08050"
                     /pseudo
     CDS             complement(892..1516)
                     /gene="recR"
                     /locus_tag="PK502_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538487.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
     gene            1924..3387
                     /locus_tag="PK502_08055"
     CDS             1924..3387
                     /locus_tag="PK502_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008658217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPH00808.1"
                     /translation="MSSTAIIILIVCYFTALFIISYFTSRRSDNQSFFIGNRRSPWYL
                     VAIAMIGTSISGVTFISVPGWVGTSQFSYLQMVLGYLLGYFVIANVLLPLYYRLQLTS
                     IYTYLDKRFGFYSYKTGAVLFLVSRIIGSAFRLFIVSSVLQLTVFDAWGIPFWVTVTA
                     TILLIWLYTRKGGVKTVLWTDTFQTITMLVAVILTIFFIAKELHLNFSGIVSTISHSE
                     LSRIWFFDDINNRFHFLKQFFGGAFITIVMTGLDQDQMQKNLSCRSYSDAKKNMYWYG
                     FAFLPVNLLFLSLGVLLYKFAEVKGISIPAQTDNLFPIIATQGYISPVVGILFVLGLI
                     SAAYSSADSALTALTTSFTIDILNTKDMDEATLQKVRKRVHLMISAVVVAVILIFNAF
                     NDRSVIDSIFTVAGYTYGPLLGLYAFGMFTRFQTNDKLVPLIALLSPIICLLLSTYSK
                     ELFGGYEFGFELVILNGLLTFTGLYFTRRKGVKIPKV"
     gene            3544..6141
                     /gene="mutS"
                     /locus_tag="PK502_08060"
     CDS             3544..6141
                     /gene="mutS"
                     /locus_tag="PK502_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPH00809.1"
                     /translation="MVETPLMKQYLSIKQKHPDAVLLFRVGDFYETFGEDAILASGVL
                     GITLTRRANGAASFVELAGFPHHALDTYLPKLVRAGYKVAICDQLEDPKLAKTIVKRG
                     ITELVTPGVAYNETILEHKENNFLGCIYLDKKLAGISFLDISTGEFLTSEGNMDYIDK
                     LLSNFKPKEVLVERSKLREFTEHFGDKYYTTRLDEWAYSEDAAREKLLNHFKTTSLKG
                     FGIDSLKLGVISAGAILYYLDITQHSQVEHISSISRIDEENYVWVDRFTSRNLELFTS
                     INEGAKTFIEVIDKTASPMGGRLLKRWVSLPLKNLNAINDRLNMVDYFFNNPDLRSTL
                     LDRIRGMGDTERIISKAAVGRITPREIVQLKNSLNLLADVKKACDESGEPTLLRIGEQ
                     LNLCTRIRERIEREIVADPPPLLGKGAVIANGVNSELDELRSILYSGKDYLLNLQQRE
                     AERTGIPSLKVSFNNVFGYYIEVRNTHKEKVPADWIRKQTLVSAERYITQELKEYEEK
                     ILGAEEKIQSLEQSIFLNLIHNLGEYIPSIQLNSNLVAQLDCLLSFSSCAHEYGYTKP
                     TVNQSDAISITQGRHPVIERMLPAGEEYVANDIYLDTTEQQIIIVTGPNMAGKSALLR
                     QTALIVLMAQIGCFVPAQAASIGLVDKIFTRVGASDNLSLGESTFMVEMQESASILNN
                     LSSRSLVLLDEIGRGTSTYDGISIAWAIVEYLHEHPNARAKTLFATHYHELNEMESTF
                     PRVKNYNVSIKELNDRVIFLRKLVRGGSEHSFGIHVARMAGMPPSVIKRAYEILAELE
                     KSQQNETPHKPVAEIGTRREGYQLSFFQLEDPVLKQIRDQIKTLDINNLTPLEALNKL
                     NEIKRLTGL"
     gene            6728..7144
                     /locus_tag="PK502_08065"
     CDS             6728..7144
                     /locus_tag="PK502_08065"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPH00810.1"
                     /translation="MKRIFFAILLAAACSTMVNAQSTEKVDENAPEITFTSTEYDYGV
                     VAVNSDGTRKFEFTNTGKQPLILSNVSASCGCTVVEWAKDPVKPGEKGTITVKYNTAI
                     VGTFQKSIRVFSNAKTSPVMLVIKGEVKPNEPAPHQ"
     gene            7170..8369
                     /locus_tag="PK502_08070"
     CDS             7170..8369
                     /locus_tag="PK502_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPH00811.1"
                     /translation="MPKISEKGLAMPASPIRKLVPFAEEAKRKGRKVYHLNIGQPDIP
                     TPQVALEAIRHHDLKVVEYSHSAGNESYRKGLASYYKGIGVDITHNDIIVTTGGSEAI
                     TIALMSCLNPGDEVIIPEPFYANYNSFALQAGVVVKPIVSSIQNDFALPPISEFEKLI
                     SPKTKGIIICNPNNPTGYLYSQQEIEQLATIVKKHDLYLFSDEVYREFCYDGNTHFSV
                     MRLTGLDNNIVLIDSVSKRYSMCGVRIGALISRNKEVIATAMKFAQARLSPPSFGQIA
                     AEAALNTPPEYFKEVYDEYINRRNFMVDALNKMEGVYCPKPKGAFYSVVKLPIDDSDK
                     FAQWLLESFEYKNQTVMVAPATGFYSTKGLGKNEVRIAYVLNTEDLRNAMECLEVALK
                     QYPGRKL"
     gene            8597..>9316
                     /locus_tag="PK502_08075"
     CDS             8597..>9316
                     /locus_tag="PK502_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPH00812.1"
                     /translation="MKFFSTILLIFNFLAATSQDKTTVEAGSEQVPEYLPEILGRNVA
                     VVANHTSLVKGVHLVDTLLSSGVKVRRIFSPEHGFRGNFEAGIPVASSTDYQTGLPVV
                     SLYGGSKQPTKEQLRDIEVVLFDLQDVGVRFYTYLSTLHYMMEACAINNVRLIVLDRP
                     NPNGFYVDGPVLDTANRSFVGLHPVPLVYGMTVGEFALMINGEGWLSKSRKCNLSVIP
                     CKGYSHRITMPLPVKPSPNLPN"
BASE COUNT         2542 a         2314 c         2072 g         2388 t
ORIGIN      
        1 aaaagagttg aacattcagc tttggggaag atgaattaca tgtaaaacta gttttttccg
       61 aaatagccct acctaccccc gcactcggtt aaaatcatct tgcaggcatt catgtcgccc
      121 agcttgcagg ccatgtggaa atcggcgcag gctttctgct tttcggaaag tttcaggtag
      181 gtcatacccc tgttgtagta ggtaagggca gagtttggtt caagatcaat cgcgttggtg
      241 tagtcgttaa cggcatcaat gtagaagcca agcctaaagt taaccaaccc ccggtaggta
      301 tagatgctgg tattgcgggg atcgagctcc atggcggcgt tgtagtcggc aagggcatcg
      361 gtaaaattgt ccagcctgat ttcagcagaa gccctgttca tcaggatctg tacggacgtt
      421 cggttggagg gtttaagctc cagcgcgtgg gaaaaggcag aaatagcctc ctcatggttg
      481 cccttgaggt tgtatataat tccctgcaac aggtgaaact cgggataggt agggaacttg
      541 ccaagtgcaa tatcggcata ccgctgggca ttcttgtaat cttccgaaag agtgtagcat
      601 ttgattaccc cctgtagggc aacggtatcg tcaggttgaa tctcaagggc ctttttgaat
      661 tgatcggcgg cagccttata gtttttttcc ttcaagttca ggtttgccga gcttaccagc
      721 tcgttgaagc tggtttgggc aatactgtgg ctagaaataa ggagtagcag gagaatgcgt
      781 ataaatcgat ttcccatagc taaagggatt tgctggatga tatgttttac caaattacca
      841 atcataaagt tacaccaaaa ttcgtgatgc caaaacaaat ttttcctcta gctaatccgg
      901 gatagaagca tcgaacttca ccctgttgag gattgccctg cccagggtta cctcgtcggt
      961 aaactccagc tcgtcaccaa aagccacccc tcgtgccaac gtggaaaata aaacaggctt
     1021 gtccttcaac tttttaaaga tgtaaaaatt agtggtatcg ccctcggtgg tagcgctaag
     1081 agccattaac ctctcttact cctccctggt ttacccggtc aaccagcttt tcgatgctca
     1141 ggtcggcagg gccaatcccc tccatggggc ttatcacgcc tccaagaaca tggtataacc
     1201 cgttgaactg gtgggtgctc tcaatcatca tgacctcctt aatgctctcc accacgcaaa
     1261 taagccccgc atcgcgatgg ggcgatgagc aaatgccgca agtatccgta tcggatatat
     1321 tgttgcacac cctgcagtac ttcacattat gacccagatc ggttatggcc ctggtgaagc
     1381 tatcaacatc ctctatgtcc tgacgtagca gatggagcac caacctcagg gccgaacgcc
     1441 tgcctattcc ggggaacttt gaaaattctt caacagcagt ttccagcaac tttgagggat
     1501 atgactggac attcatactc tattctttat tcatttttat caacagcctt gcaaccatca
     1561 atttttttgg cacttcgtac tcccttaggc tattcccatg ttcaacttgg gacacagcca
     1621 ctcacaatcg gaaagttggg ttcgataaaa tttaaagttt ccacaaatct acatgaattt
     1681 agaattaaat ggcaaattcc tccccgtatt aaaggaaaat aagatgaaat accagcttta
     1741 tcccttcaag caggagatta cctgaaagaa ttacccaagc aaaaacaaaa ttttggaatt
     1801 tatgcttggc ggatgagcag tataaaacga atccccattc acctaatcaa aaaccgctgt
     1861 cccgaaccct agcatatttt ggcttaaatt gattaaactt gcagcgccta aaaccaggag
     1921 tttatgtcgt caactgcaat tatcattctg attgtctgct acttcacagc actctttatt
     1981 atttcctact tcacctccag gcgttccgat aaccagtcgt tttttattgg taacaggcgc
     2041 tcgccttggt acctggtggc tatcgccatg ataggaacat ccatttcggg agttaccttt
     2101 atttcggtcc ccggatgggt tggaaccagc cagttctcct acctgcaaat ggtgctgggc
     2161 tacctcctcg gctactttgt tattgccaac gtgctgctgc cattatacta ccggcttcag
     2221 ctaacatcaa tatacaccta cctcgacaaa cgctttgggt tttactccta caaaaccgga
     2281 gcggttcttt tcctggtatc caggataatt ggctcggcgt tccggctgtt catcgtttcg
     2341 agcgtgctcc aacttacggt tttcgacgcg tggggcattc ccttttgggt aaccgtaacc
     2401 gccaccattc ttttaatctg gctctacacc cgtaaaggag gggtgaaaac cgtgctctgg
     2461 accgatacct tccaaactat taccatgctt gtagcggtta tccttactat ctttttcatt
     2521 gccaaagagc tacacctgaa tttttcaggg atagtaagca ccataagcca tagcgagcta
     2581 tccagaatct ggtttttcga cgatatcaac aaccggtttc acttccttaa gcagtttttt
     2641 ggaggagcgt tcatcacaat agttatgacc ggccttgacc aggaccaaat gcagaaaaac
     2701 ctgagctgcc gaagctactc cgacgccaag aaaaacatgt actggtacgg gtttgccttc
     2761 ctccccgtta atctcctttt cctttcgctg ggagttctgc tatacaaatt tgcagaggta
     2821 aagggaataa gcatacccgc ccaaaccgac aacctattcc ccattatagc cacgcaaggc
     2881 tacatttcac cggttgttgg aattctcttt gtgctggggt tgatttctgc tgcctactcc
     2941 agcgccgact cagcccttac tgctcttacc acctcgttca ctatagacat cctcaacaca
     3001 aaggacatgg atgaggctac acttcaaaag gtgcggaaac gggttcattt aatgatatct
     3061 gctgttgttg tagccgtaat tcttatcttc aacgcattta atgaccgaag tgtaattgac
     3121 tccattttta ccgtagctgg ttacacctac ggcccccttc ttggactata cgcttttggc
     3181 atgttcaccc gttttcaaac aaacgataag ctggttccgc taatagccct gctgtcgccc
     3241 atcatctgtt tattgctaag cacatactcc aaggaacttt ttggcgggta cgagttcgga
     3301 tttgagctgg taatcctgaa cggcctccta acctttacgg ggctttactt cacacgccga
     3361 aaaggtgtaa aaattccgaa ggtatagccc tttttcatcc cattgcccct actcatttaa
     3421 tcaggctaaa actgctaatc cggtttacca aaatgttaca ttttaagctg aaacttggtg
     3481 aggatttgat aattttgtgg gtaaacaaaa gcaaacacct tggacgaagg caggaataag
     3541 ggcatggtgg aaacacccct gatgaagcag taccttagca ttaagcagaa acatcccgat
     3601 gcagtgctac tctttagggt gggcgatttc tacgagacct ttggagagga tgccatcctg
     3661 gcatcaggcg ttctcggtat aaccctcacc cgaagggcca acggagcagc ttcttttgtg
     3721 gagctggcag gatttccgca ccatgccctc gatacctacc tcccaaaact cgttagggca
     3781 ggctataagg ttgccatttg cgaccagctg gaagacccaa aactggccaa aaccattgtg
     3841 aaaaggggca tcaccgagct ggtaacgccc ggtgtagcat acaacgaaac catacttgag
     3901 cataaggaaa acaactttct gggttgcata tacctggata agaagttggc tggcatctcg
     3961 ttcctcgaca tatcaacagg tgaattccta acctccgagg gcaacatgga ttacattgat
     4021 aagctgctaa gcaactttaa acccaaagag gtgctggttg aacgctcgaa actgcgcgag
     4081 tttaccgaac atttcggcga taagtattac accacccggc tcgatgaatg ggcgtacagc
     4141 gaggatgccg ctcgcgaaaa actccttaac cactttaaaa caacctcgct caagggattc
     4201 ggaattgata gcttaaaact gggagtaatc tcggcgggag ccatactcta ctaccttgac
     4261 atcacgcaac acagccaagt tgagcatatc tcaagcattt cgagaattga tgaggaaaac
     4321 tacgtttggg ttgaccgctt tacttcaaga aatctggagc tgttcacctc cattaacgag
     4381 ggggctaaaa cattcatcga ggttattgat aaaaccgcat cgccaatggg tggtaggtta
     4441 ttaaaacgat gggtttcgct acccttgaag aatttgaatg ccattaatga caggctcaac
     4501 atggtggatt acttcttcaa taaccccgat ttaagaagta cactgctgga tagaataagg
     4561 ggtatgggcg acaccgagcg aattatatca aaagccgccg ttggtcgtat taccccccga
     4621 gagatagttc agctgaaaaa ttcgctcaac ctacttgcag acgtaaagaa ggcctgcgat
     4681 gaatcgggcg agcccaccct tttgcgcatt ggggagcagc taaacctttg cacccgaatt
     4741 cgcgaaagga ttgaaaggga gattgttgcc gatccacccc cgcttctggg caagggagct
     4801 gttattgcca acggcgttaa cagcgagctg gatgaactcc gtagcatcct ttactccggg
     4861 aaggattatt tgttgaattt acaacaacga gaggctgaac gaactggcat accctcgcta
     4921 aaagttagct ttaacaacgt atttggctac tacatcgagg ttaggaatac ccacaaggag
     4981 aaggttccag ccgattggat acgtaagcaa acccttgttt cggcagagcg ctacattacc
     5041 caggagctta aggaatacga ggagaaaatt ctgggtgcag aggagaaaat ccaatccctt
     5101 gagcagtcaa ttttcctgaa tcttatccac aacttgggcg agtacatccc ctccattcag
     5161 ctaaactcca accttgttgc ccagctcgat tgcttactgt cgttttcaag ctgcgcccat
     5221 gagtacggat acactaaacc aacagttaac cagagcgacg ccatctcaat aacccagggc
     5281 aggcatccgg tgattgaacg catgctaccg gcaggcgagg agtacgtggc caacgacatc
     5341 tacctcgaca ctaccgagca acagattatt attgttaccg gaccaaatat ggcaggaaaa
     5401 tcagccttgc tcaggcaaac cgccctgatt gttctcatgg cccagatagg ctgttttgtt
     5461 ccggcgcaag ctgcaagcat tgggctggtt gataaaattt ttacccgggt tggtgcctcc
     5521 gacaacctct cgctcggtga gtcaacattt atggtcgaaa tgcaggaaag cgccagtatt
     5581 ctcaacaacc tctcgtcccg tagccttgtg cttcttgacg aaataggtcg gggaacctca
     5641 acatacgatg gcatttccat tgcatgggct attgtggaat acctgcatga gcatcccaac
     5701 gccagagcta aaaccctttt tgccacccac taccacgagc tgaacgaaat ggagtccacc
     5761 ttcccccgtg tgaagaacta caacgtctcc ataaaggaac tcaacgaccg ggtaatcttt
     5821 ttgcgcaagc tcgttcgcgg cggaagcgag cacagttttg gcatccacgt tgcgcgtatg
     5881 gccggcatgc cacccagcgt gattaaaagg gcctacgaaa tacttgcaga acttgaaaaa
     5941 tcgcaacaga atgaaactcc acataaaccg gtagcagaaa ttggaacccg gagggagggt
     6001 taccagctga gcttcttcca gctcgaggac cccgtgctga agcagattcg cgaccagatt
     6061 aaaacgctgg acatcaacaa ccttaccccg ctggaggcgc taaacaagct gaatgaaatt
     6121 aagcggctta ccggcctcta gccaccgatt gctattctac atctgctaac atccgagcat
     6181 cccataaaaa ccaaagaaac ctacccttga tttcagaaaa gtagatctag cagattccct
     6241 gtttggatta ggctgtgtgc ctcggtcgta tctatctctt cgggctctgc cctgtaaata
     6301 tagctaattg taagttttcc accgtatgtc atttcaagaa tagcaggctg atcccgcttt
     6361 cgatagccca acggcggtgc aaccaaccgc agactacgaa ttgtttatta ctttttatag
     6421 cctcagccaa acataaaaca actaaaaaaa tggtcgcagc ctaaaagaat agctcgacgg
     6481 atgaacgctt tgctaaactt tgcccaaccc agtgcacttc tttatcaagt tgtagccttg
     6541 gcccagctgt gggattgtac aattacattt attaacagta gtttaaacca aatagctaaa
     6601 agaaccctaa ctttgcaacc tgaaatgcac tgccaacttc gtgcaatttc aactcagcct
     6661 aaagctcctc tgataaaaag tgtgccttac aatatattgt ttaacttttt gctaaaacga
     6721 gattacgatg aaaagaattt ttttcgccat tttgttggcc gcagcttgca gcacaatggt
     6781 taatgcacaa agcaccgaaa aggtggacga aaatgctcct gaaattacct ttacatcaac
     6841 ggagtacgac tacggggtag ttgccgtaaa ttcagatgga acccgtaaat tcgagtttac
     6901 gaacaccggg aaacaaccct tgattttaag taatgtttcg gcatcatgcg gctgcacggt
     6961 tgtggagtgg gccaaggatc ctgttaagcc aggcgaaaag ggaaccatta ccgttaagta
     7021 caacacagcc atagttggaa cattccagaa aagcatacgc gttttctcca acgccaaaac
     7081 cagtccggtt atgctagtaa ttaagggtga ggttaaacca aacgagcctg caccacatca
     7141 atagattaca aaccaaatca cataaagaca tgccaaaaat atccgaaaag gggctggcta
     7201 tgccagcttc tccaatccga aagctggttc cttttgccga agaggcaaaa cgaaaggggc
     7261 gtaaggttta ccacctgaat attggacagc ccgatattcc tactcctcag gtagcgcttg
     7321 aggctattcg acatcacgac ctaaaagtgg ttgagtatag ccactcggca ggaaacgaaa
     7381 gctaccgcaa ggggcttgcc agctactata aggggattgg cgttgacatc acccacaacg
     7441 acatcatcgt tacaacaggg ggatcagaag ccattaccat tgcgctgatg tcctgcctga
     7501 acccgggtga cgaggtgatt atccccgagc cattctacgc caactacaac agttttgccc
     7561 tacaggcggg agttgtggtt aaacccattg tttcgtccat acaaaacgac tttgcgcttc
     7621 ccccaatttc ggagtttgaa aagctcatct cacctaaaac caaaggcatt attatctgca
     7681 accccaacaa ccccaccgga tacctttact cccaacagga aattgagcag ctggcaacca
     7741 ttgttaagaa gcacgacctc tacctattct ccgatgaggt gtaccgtgaa ttttgctacg
     7801 acgggaatac ccatttttca gtgatgaggc tcaccgggct tgataacaac attgtcctaa
     7861 ttgattccgt ttccaaacgc tacagcatgt gtggcgttcg gattggtgcc ttaatttcac
     7921 gtaacaagga agtgattgca accgccatga aatttgcaca ggcacggctt tcaccgccat
     7981 ccttcgggca aattgctgcc gaagctgccc tcaacacgcc accagagtat tttaaggagg
     8041 tatatgacga gtatatcaac cgtcgtaact tcatggtgga tgcccttaac aagatggagg
     8101 gggtttactg ccccaaacca aaaggagcct tctacagcgt tgtgaagctg ccaattgacg
     8161 acagcgataa gtttgcccag tggctcctcg aaagttttga gtataagaac caaaccgtca
     8221 tggtagctcc tgcaaccggt ttttactcca caaaaggatt gggtaaaaac gaggtgcgta
     8281 ttgcctacgt gctgaacacc gaggatttga gaaatgccat ggaatgtctc gaggtagccc
     8341 ttaagcagta tcccggtcgt aagctttagc agagcttgaa ataagtgcaa aataaagggt
     8401 ggcctgcgga accacccttt ttgtgcttta taaaccatgt atttcagctt cactaggcgg
     8461 aatcaaaaaa tatttctact ttagcacaag ggttttaaac ttggcattta aggtatccta
     8521 tccaaatgca tatctacgct accctaagca ttccggacta tttaaactat cagaatcagc
     8581 aacaactcgc catcctatga agtttttttc aaccatattg cttattttca actttttggc
     8641 ggcaacttca caggacaaaa ctaccgttga agccggatcg gagcaggtgc cggagtacct
     8701 tcccgaaatc ttggggcgaa acgttgctgt ggtggccaac cacacctcgc tggtgaaagg
     8761 agtgcacctg gttgacaccc tgctgtcatc aggtgtaaag gtgaggcgaa ttttcagccc
     8821 ggagcacggt ttccgtggta actttgaagc gggaatacct gttgcaagca gtaccgacta
     8881 ccaaacagga ctgcctgttg tttcgctcta tggtggtagc aagcagccca ccaaggagca
     8941 acttcgcgat attgaggtgg tgctcttcga cctgcaggat gttggcgtga ggttctacac
     9001 ctacctatca accctacact acatgatgga agcatgtgcc atcaacaacg tcaggttaat
     9061 agtgcttgac cggcctaatc ccaatggatt ttatgttgat ggccctgtgc ttgataccgc
     9121 caaccgttcg tttgtagggc ttcatcctgt tccgctggtt tatggaatga ctgttggtga
     9181 gtttgccctg atgataaacg gcgagggatg gctttccaag agtcgtaaat gcaacctgag
     9241 tgttatcccc tgcaaaggat acagccaccg aataaccatg ccccttccgg tgaaaccatc
     9301 gccaaattta cccaac
//
LOCUS       DAOPHX010000102         9072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_9589_length_9072_cov_6.218476, whole genome shotgun sequence.
ACCESSION   DAOPHX010000102 DAOPHX010000000
VERSION     DAOPHX010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9589_length_9072_cov_6.218476"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PK502_08080"
     CDS             <1..359
                     /locus_tag="PK502_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPH00813.1"
                     /translation="VIPSWTDDMDMIRRMCGWLVENGFSENPLHFSRFFPLYKLTQLP
                     QTPVSTLIRARDIALKAGLKHVYIGNVPSMDASNTTCPNCGKVVLERRGFSIYSINLK
                     NGKCAYCGSMVKGVWE"
     gene            complement(620..2149)
                     /locus_tag="PK502_08085"
     CDS             complement(620..2149)
                     /locus_tag="PK502_08085"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPH00814.1"
                     /translation="MGSWTNILSSIFSYTEGNPLLFTEMSFWVFFAVLLTGYSFIYNR
                     PLARSAYLLAFSLYFYYKSSGLFFFLLIFSTVCDYTIGLLIPRYSSKTARKLLVALSV
                     IINLAVLSYFKYAYFITETVNQLFGLNLEVVNYLSGWANGIFGSHFDTSTIILPAGIS
                     FYTFQTISYTVDVYRRKVAPVKNIIDFGFYVSFFPQLVAGPIVRAAEFIPQLYRKFSL
                     TYREFGHALFLIIAGLVKKMVISDYLSVNFVDRVFDNPLSFSGFENLMATYGYALQIY
                     CDFSGYTDIAIGVALLLGFRLPPNFNSPYKAVSIADFWHRWHISLSTWLRDYLYIPLG
                     GSRKGKVRTHLNLMVTMLLGGLWHGANLKFVVWGGIHGVSLVLHKLWTSHSPRVKRAD
                     SWWRRAIGAFFTFQLVSFSWIFFRSSTLSSAFDMLGQIFTRFGTPSLPQMLLYYAKPF
                     ALMLAGFAIHWLPFRVSEGVRGWFIETTVLVKLLITVMAVLLILQLNSAELQPFIYFR
                     F"
     gene            complement(2236..3762)
                     /locus_tag="PK502_08090"
     CDS             complement(2236..3762)
                     /locus_tag="PK502_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00815.1"
                     /translation="MIKPFKTLLFLLSVFIVFLGISFFFPSGKKNIAKITLYVPSTDS
                     LLGAITSKPAMAEQPEANKKPPIMLNAEVDSNKTVLPTPENGEFKKSMMKTDTLTRNT
                     SLAYSTNSTATDKIVPIEFTTEAMGSFFAALKNGEAGSSQVRVLHFGDSQVEGDRISD
                     YLRSVLQEKFGGYGPGILPAYPQNYQPYGIRQSASKGWNYSNLLQPNEMVRKYGILGG
                     LSETNDKDATEYIRFKKNARDARNALFDRIRVFYGKNDGPCMLQLLKDGEVADTEILQ
                     QAQTLAQHSFTISPDAKTVELQLANTGHILIYGLSLESERGIYVDNIPLRGSSGEIFT
                     RFDHELARQLFGMLNVKLVILQFGLNVAPGNLTSYDYYEERLYKQVMAIKSFKPDVSV
                     LIIGISDMLRRVDGRLETYPSIEKIRDAQRSAAFRSGAAFWDCYRAMGGKNSMLTWVN
                     SNPPLGSMDYAHFTYRGARVIAGKLTKSLLEAFDRYSFPESSAIPTEAIARPDSSQGG
                     "
     gene            3904..4248
                     /locus_tag="PK502_08095"
     CDS             3904..4248
                     /locus_tag="PK502_08095"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HPH00816.1"
                     /translation="MLKIYHNPRCAKSRKGLEYLSSRGIEFEVKNYMKEGLRADEVRE
                     IFLKSNIKPSQLARTQDDYYKMYLKGKNFSDDEWVQIYVDNPAILQRPIVVGRYKAVL
                     AIPPENMDVLFK"
     gene            4266..5126
                     /locus_tag="PK502_08100"
     CDS             4266..5126
                     /locus_tag="PK502_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPH00817.1"
                     /translation="MKKKFVLIFLASVLMSGFLPAQNENELSISQLENEVHRLVNAHR
                     KSIGLGELAPNSYIRKEAYQHSLNMAEGKVPFSHKGFEERFERLSGFLDITSGAENVA
                     NGYNSAQDIVDGWLLSPGHKKNIEGDFNLTGIGIAQAPNGTYFYTQIFVKSQERPKIV
                     ASDFESELLGLINEHRKGLGLSALKSNDVVKAEAVNYSGQMAAGRAPIGPPGYDNPMK
                     ALLSKMNAVAMTEIIAYNYYTAKEVFNSWMASTRQRETIEGSFDLTGIGVVQSADGKV
                     FVTQIFLLSR"
     gene            5144..6766
                     /locus_tag="PK502_08105"
     CDS             5144..6766
                     /locus_tag="PK502_08105"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPH00818.1"
                     /translation="MAVPEFYYQERFPLGEEETEYYLLTRDFVSLSQFEGKDILRVDP
                     QGLELLARTAMHDTSFMLRTGHVRQLAAILNDPEASKNDKFVALVLLRNAEIAAKGIL
                     PLCQDTGTATIVAKKGQRVWTGADDYEVLSRGIFQTYTKESLRYSQTVALDMYSEKNS
                     GTNLPAQIDIYATTGEEYEFLFIAKGGGSANKSMLFQETKALLNPEKLEAFLIEKIKN
                     LGTAACPPYHIAIVVGGTSAEETLKTVKLASAKYLDNLPVKGNEFGQAFRDLELEEKI
                     FRAANSIGLGAQFGGKYLALDVRIIRLPRHGASCPVGIGVSCVADRNVKAKINRHGIW
                     VEKLENNPGRFIPEQWRKPDEEGAVGIDLNQPMETIRAELSKHPVGTRLSLSGKIVVA
                     RDIAHARLKERMDRGEGLPQYLKNHPVYYAGPAKTPEGMPSGSFGPTTAGRMDSYVDA
                     FQSQGASLVMIAKGNRSAAVTEACKKYGGFYLGSIGGPAAILAHENIKKVELLEYPEL
                     GMEAVYMIEVSDFPAFILVDDKGNDFYKQVAR"
     gene            6934..8523
                     /locus_tag="PK502_08110"
     CDS             6934..8523
                     /locus_tag="PK502_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00819.1"
                     /translation="MRKLGFILLFFCLLCSAGYSQTTYHHISNFGVYEFIDELASLKV
                     VELYSTVKPYSRAQIAEALKQADAKRSELNRRQQGELDFYLADFGKELGSFPKRKRFD
                     LLYRKDSLFSITLNPLLGVDLISNDSALAYHRWVGAEVHGHVAGWGYYASLRDYAENY
                     RLAMPTYLTRQQGGNYKYSGKGGEFSEMRGGVTYSWRWGNVGVVKDNIQWGSGYNGSN
                     ILGGRTPSFAMIKLNITPARWVELNYLHGWLVSEVIDSSRTYTSGDEVRKVYHPKYIA
                     ANLIAFKPFRGLSLSVGNSIIYSDINVQPAYLIPFLFYKSIDHHLNGMSNSVGQNSQM
                     FFDISSRNIRHLHLYATLFLDEISMKNMFNPDMHSNFYSLKTGVRISDFPFSNLIVTG
                     EYTRTNPLTYRHIINTTTYESNNFNLGHYLQDNADEIYVSLLFRPIRGLQVSMSYSVS
                     RKGPDYSTITTTRLGLPFMESVEWELREWAVGAKYEVINDGYIYLSGGVSDITDRVGN
                     YTPDFMVGRQAQLRAGVCFGF"
BASE COUNT         2437 a         2024 c         2299 g         2312 t
ORIGIN      
        1 tggtgatccc gtcgtggacc gacgatatgg acatgatcag gcggatgtgc ggctggctcg
       61 ttgaaaatgg attttcggaa aacccgcttc atttcagccg gttttttccg ctttacaagc
      121 tcacccagct tcctcaaacc cctgtttcaa cgctgataag ggccagggat attgccctga
      181 aggcggggtt aaagcatgtt tacattggca atgtgcccag catggatgcc tcaaacacta
      241 cctgcccaaa ttgcggcaag gttgttttgg agcgcagggg gttttccatt tacagcatca
      301 accttaaaaa cgggaagtgc gcatactgcg gctcaatggt gaaaggtgtg tgggagtgaa
      361 catccagcgc atttcccaat cggaagtagc tgcctttgag cggtggttta ttgatagttg
      421 ccggaaggtt tgttataaac agccttgagc tggagcatgg gtccaaactt gaggctgagt
      481 gccagtatgg tgttaagctg gcggaatttt tctaaatcgt cctaagtgta cttggggatg
      541 cattttgatc ctgttgaggg gagcagctta tgttttcatt catccattta tcatcaatat
      601 ataacaagat accatcattt taaaacctga agtagatgaa cggctgaagc tctgctgaat
      661 tgagctggag gatgagcagc acagccataa cagtaatcag tagttttacg agaactgtag
      721 tttcgatgaa ccaacccctt accccttcgc ttaccctgaa cggcagccaa tgaatggcaa
      781 agcctgcaag catcagggca aatggtttgg catagtatag cagcatctgc ggaagggagg
      841 gggtgccaaa tcgggtgaat atttggccta gcatgtcgaa ggcggagcta agcgttgaag
      901 agcggaagaa tatccaggag aatgatacca gctggaaggt aaaaaatgcg ccaatcgccc
      961 ttctccacca gctgtcagca cgtttgactc tgggcgaatg tgaagtccaa agcttatgca
     1021 gaacaaggct aaccccgtgg atgccgcccc aaaccacaaa cttcaggttg gcaccatgcc
     1081 ataacccacc cagcagcatg gtaaccatca ggttgaggtg tgttctgacc tttcccttac
     1141 ggctaccgcc aagcggaatg tagaggtagt cgcgaagcca tgtggacagc gagatgtgcc
     1201 acctatgcca gaagtcggca atgctgactg ctttataggg cgagttgaag tttggcggga
     1261 gtctgaagcc tagtaaaagg gcaaccccga tggcaatatc ggtgtatccc gagaaatcgc
     1321 agtatatctg cagggcatag ccgtaggtgg ccatcagatt ttcaaatccc gagaagctaa
     1381 gcgggttatc gaaaacccta tccacaaagt tgaccgaaag gtagtccgat atgaccattt
     1441 ttttcaccaa ccctgcaata attaggaaaa gcgcatgacc aaactccctg taggtcaggc
     1501 taaactttct gtagagctgg gggataaact cggctgccct cacaatgggg ccggccacca
     1561 gctggggaaa gaacgaaaca taaaagccaa agtcaatgat gttcttaaca ggagccacct
     1621 tgcggcggta aacgtcaacc gtgtagctga tggtttggaa tgtgtagaac gaaatgccgg
     1681 ctggcaggat gatggttgag gtgtcgaagt gggaaccaaa aattccgttt gcccaccccg
     1741 aaaggtagtt gaccacttca aggtttaagc caaacagctg gttaaccgtt tcggtgatga
     1801 agtaggcata cttgaagtag gagagcacgg caaggttgat gataacgctt agcgccacca
     1861 gcagcttacg ggcggttttt gagctgtagc ggggaatgag tagcccaatg gtgtagtcgc
     1921 atactgttga gaagatgagc aggaagaaaa acaatccact cgatttgtag tagaagtaaa
     1981 ggctgaacgc aagcaggtat gcgctgcggg ccaacggccg gttgtagatg aaagaataac
     2041 ccgtaagcag tacggcaaaa aacacccaga acgacatctc ggtaaatagc aggggattgc
     2101 cctcagtgta gctgaagatg cttgacagta tgttggtcca tgatcccatt cttttctttt
     2161 aggtctaatc gtttttgata tcttccaccc gtggtgattt ttttcttatg gcaaaatgct
     2221 gacagattat tcagctcaac caccctggct actgtcgggt ctggcaattg cttcggttgg
     2281 tatagcggag ctttcgggga aactatacct atcaaaggct tcaagcagcg attttgtaag
     2341 tttcccggca atcaccctgg cccccctgta ggtgaagtga gcgtaatcca ttgacccaag
     2401 cggaggattg gaattgaccc atgtgagcat ggagtttttc cctcccattg cccggtagca
     2461 atcccagaaa gccgcacccg acctgaaagc cgcactccgt tgagcatccc tgatcttctc
     2521 gatgctggga taggtttcga gccggccatc aaccctgcgc agcatatccg aaatgccgat
     2581 gatgagaacg gaaacatcgg gtttaaacga tttgatggcc attacctgct tgtagagacg
     2641 ctcttcgtag tagtcgtagc tggttaggtt gcccggggct acattcaaac cgaactgcag
     2701 gataaccagc ttcacgttaa gcataccgaa cagctgcctg gccagctcat ggtcgaatct
     2761 cgtaaaaatt tcacccgagc ttccccttag gggaatattg tcaacgtaaa taccccgctc
     2821 cgattcaagc gatagaccat atatcaggat atgacccgtg ttggcgagtt gaagctcaac
     2881 ggttttggca tcggggctaa ttgtgaagga atgctgtgcc agcgtttgag cttgttgaag
     2941 tatttcggta tcagccacct ctccatcctt cagaagctgc agcatacagg gaccatcatt
     3001 ttttccatag aatactctta tcctatcgaa cagggcattt ctggcatccc ttgcattttt
     3061 cttgaagcga atgtactcgg tagcatcctt atcgttagtc tccgataatc cacccaggat
     3121 accatatttc ctcaccatct cgttgggttg gagaaggttg gaataattcc acccttttga
     3181 cgccgattgc ctgattccgt aaggttggta gttttgtggg taggcaggaa gaatgcctgg
     3241 gccgtaacca ccaaactttt cctgtaacac cgaacgtaga tagtcagaaa tccgatcgcc
     3301 ctcaacctgt gagtcgccaa agtgtagaac cctaacctgg gagctgccgg cctccccatt
     3361 ttttaaagcg gcgaagaagc tacccatggc ctcggtagta aattcaatag gaacaatttt
     3421 gtcggtagca gtgctgtttg tggagtatgc caacgacgta ttccttgtca acgtgtcggt
     3481 tttcatcatt gattttttga attcgccatt ttcaggggtg ggtagcacgg ttttatttga
     3541 atctacttca gcattgagca ttatgggtgg tttcttgttt gcttctggtt gttctgccat
     3601 tgctggtttt gaggtgatgg ccccaagcag gctgtcggtg gaaggaacat acagggtgat
     3661 tttggcaatg tttttctttc cgcttgggaa gaaaaacgaa attcctagaa acacgatgaa
     3721 aaccgatagc agaaacaaaa gggttttaaa aggcttaatc atttatcgta gcttttagtg
     3781 gcattttttc tgtaataccc gtataaaagt aaggtaattt tgtaaaattg ctaattttac
     3841 atataagaat tatagcgggt aaaaccatcg ccattttttt gtaactttca aaaaaaatat
     3901 tttatgctga aaatatacca taaccctcga tgtgcaaaga gcagaaaggg tttagagtat
     3961 ttgagtagca gggggataga gtttgaggtt aaaaactata tgaaggaggg gctccgggca
     4021 gatgaggttc gagagatatt cctaaagtcg aatattaaac caagccagct agcccgaact
     4081 caggatgatt actacaagat gtatttgaag gggaaaaact tttccgatga tgaatgggtt
     4141 caaatttatg ttgataaccc cgccattctc caacgaccca tcgtggtggg caggtataaa
     4201 gccgtgctgg ctattcctcc tgaaaatatg gatgtactct ttaaataaat aaatagtttg
     4261 tcctgatgaa gaagaaattt gtcttgattt ttcttgcctc cgtccttatg agcggatttt
     4321 tgccagctca gaacgaaaat gagttgagca tttcacagct ggagaacgag gttcaccgcc
     4381 tggtaaatgc gcatagaaaa tctattgggc tgggagagct ggctcctaat agctatattc
     4441 gaaaggaggc ctaccagcac tcgttgaaca tggccgaagg taaggttccc tttagccata
     4501 agggttttga agagcgattt gaacggctaa gcggtttttt ggacattact tccggggccg
     4561 aaaatgttgc caacggctac aactcggctc aggacatagt cgatggctgg ttgttaagcc
     4621 ccgggcataa aaaaaatatt gagggtgatt ttaacctaac cggaattggg attgcccagg
     4681 cgcccaacgg cacctacttc tacacccaga tatttgtcaa aagtcaggaa cgccctaaaa
     4741 ttgttgcatc agactttgag tcagaactat tagggctgat aaatgagcat cgtaaaggat
     4801 tagggcttag cgcactgaaa agcaacgatg tggttaaggc cgaagcggtg aactactccg
     4861 ggcaaatggc agccggtaga gcgcctatag gtcctccggg gtacgataac cccatgaagg
     4921 cgttgctttc aaagatgaat gctgttgcca tgaccgaaat aattgcctat aactactaca
     4981 ctgccaagga ggtgtttaac agctggatgg ccagcaccag gcaacgagag accatagagg
     5041 gaagcttcga ccttaccggc attggagttg tgcagtctgc cgatgggaag gtttttgtta
     5101 cgcagatttt tctacttagt cgttaacata aaattcaacc aaaatggctg ttcctgaatt
     5161 ttactaccag gaaaggtttc ctttgggaga ggaggagacc gagtactacc ttctaaccag
     5221 ggattttgtt tccctttcgc aatttgaggg taaggatatt ctaagggttg acccgcaggg
     5281 cctggagctg ttggccagaa cagccatgca cgacacatcg ttcatgcttc gcaccgggca
     5341 cgtcaggcag ctggcagcta ttcttaacga ccccgaggct agtaagaatg acaagtttgt
     5401 tgcccttgtt ctgcttagga atgccgaaat agccgctaag ggcatacttc ccttgtgtca
     5461 ggatacggga actgcaacaa ttgtggctaa aaaggggcag cgggtatgga caggtgccga
     5521 cgattacgaa gtcctttcaa ggggaatttt ccaaacctat accaaggaga gtctgcgcta
     5581 ctcgcaaaca gttgcgctgg atatgtactc cgagaaaaac tccgggacaa atcttccagc
     5641 acagattgac atctatgcca ccactggcga ggagtacgaa ttcctgttca tcgccaaggg
     5701 cggaggttca gccaataaga gcatgctatt tcaggaaaca aaggccttgc ttaaccccga
     5761 gaagctggag gcttttctta ttgagaaaat aaagaacttg ggaaccgctg cctgcccacc
     5821 ctaccatatt gccattgtag ttggcggcac atcggccgag gaaaccctaa aaacggtaaa
     5881 gctagcctcg gctaagtatc tcgacaacct gcccgttaag gggaatgagt ttgggcaggc
     5941 tttcagggat ttggagcttg aggagaaaat tttccgtgca gccaacagta taggacttgg
     6001 tgcacagttc gggggcaagt accttgccct ggatgttagg attatcaggc ttccccgcca
     6061 tggcgcatcc tgtccggtag gaatcggggt atcatgcgtg gccgatagga atgtaaaggc
     6121 caagataaac cgccacggta tctgggttga aaagttggaa aacaacccgg gaaggtttat
     6181 ccccgagcag tggcgtaaac ctgatgagga aggcgctgtt ggtatagacc tgaatcagcc
     6241 aatggagact attcgagccg agctatcgaa gcacccggtt ggcacacgac tttcgttaag
     6301 cggcaaaatt gttgtggccc gcgatattgc ccatgcccga cttaaggagc gtatggatag
     6361 gggggagggg ctgcctcagt accttaagaa tcatccggtt tactacgccg gtcccgctaa
     6421 aaccccagag ggtatgccat cgggctcttt cggccccacc actgccggcc ggatggactc
     6481 ctatgttgat gcattccagt cgcagggagc aagcctcgtg atgattgcca agggtaacag
     6541 gagcgccgca gttactgagg cctgcaagaa atatggaggc ttttacctgg gaagcatagg
     6601 cggacctgct gccattcttg cgcacgagaa tattaaaaag gtggagctgc tggagtaccc
     6661 cgagcttgga atggaggcag tttacatgat tgaggtttcc gactttcctg ctttcattct
     6721 ggttgatgac aagggcaacg atttttacaa gcaggtggcc aggtaatttc gatggcgctt
     6781 ggtggatttc tgctaccatt cggccaaata tcagcatggt gtttaaggaa atttggaggg
     6841 gaaatttcgt cactcatgaa tgcggcttac cgtcttgcta taagcagtat ccgcgtggct
     6901 atcaatttag taatagaatt caaatttatg gaaatgagaa agcttggttt tatcctgcta
     6961 ttcttttgtc tcctgtgctc agcgggatac tcccaaacca cctaccatca tatctccaac
     7021 tttggtgtgt acgaatttat tgatgagctg gcatcgctta aggtggttga gctttatagc
     7081 acggtgaagc catactctcg tgcgcaaatt gccgaggcgc ttaagcaagc cgatgctaaa
     7141 aggtcggagc tgaacaggcg tcagcagggc gagcttgatt tttaccttgc cgattttgga
     7201 aaggagctag gctcatttcc caaacgcaag cgtttcgacc ttctttaccg aaaagattcg
     7261 ctattcagca taaccctaaa cccgctgctg ggtgttgacc ttatttcgaa cgatagcgca
     7321 cttgcttacc atcgttgggt gggagctgaa gttcatgggc atgttgccgg atggggctac
     7381 tacgccagct tacgtgacta tgccgagaac taccggttgg ccatgccgac ctatttaacc
     7441 cggcagcagg gtggaaatta taagtattca gggaagggcg gggagttcag cgagatgagg
     7501 ggaggggtta cctatagctg gaggtggggt aacgttggcg ttgtaaagga caacattcag
     7561 tggggatcgg gctacaacgg ttccaacatt ctcggcgggc ggacaccttc ctttgccatg
     7621 atcaagctaa acatcactcc tgcacgttgg gttgagctga actacctgca cgggtggctt
     7681 gtttccgagg ttatcgactc cagccgcacc tacacctctg gtgatgaggt gcgaaaggtg
     7741 taccacccca agtatattgc cgctaaccta attgccttta aacccttcag ggggctaagc
     7801 ctgtcggttg gcaactccat tatctactcc gatattaacg ttcagcctgc gtacctcatc
     7861 ccctttctgt tctacaagtc cattgaccac cacctgaacg ggatgagcaa ctcggtggga
     7921 caaaattccc agatgttctt tgacatttcg tcgcgaaaca taagacacct gcacctgtac
     7981 gccacccttt tcctcgatga aatttcgatg aagaacatgt tcaaccccga tatgcacagt
     8041 aacttctaca gcctgaaaac cggtgtcaga atttcggatt ttccatttag caacctcatc
     8101 gtaacaggtg aatatacacg cacaaacccg ttgacgtaca ggcatataat taatacaaca
     8161 acttacgaaa gcaacaactt taacctgggg cactacctgc aggacaatgc cgatgaaatt
     8221 tacgtatcgc tgctatttag gcctatcaga ggccttcaag tttccatgag ctactccgtc
     8281 agccgtaagg ggcccgacta cagcacaata acaaccacca ggcttgggtt accatttatg
     8341 gaaagcgtgg aatgggagct acgggagtgg gcagtagggg caaagtatga ggtgattaat
     8401 gacggctata tctacctttc aggcggtgtt tcggatatta ccgacagggt tggaaactac
     8461 acccccgatt tcatggtggg caggcaggca cagcttaggg ctggtgtttg ctttgggttt
     8521 taagcacctg gctgctatgg tattatttga gaattatgct gtttgcctgc tgatactacc
     8581 cgtacctaaa cgcccgctcg tagagagccc ggtgtggcca ttggtgcgac ggtgagggca
     8641 caagagttca acaacagctg aaatttatac ccatgcttca caaaaaaaat tgcagaaaat
     8701 taggaaccca tttgatgagt tgtaacatta tactacattt gtttatagaa atagaacgca
     8761 acatcatagc gcctatacat ccaaaaatgg aaataatgcg gaacagagtg gcgcctataa
     8821 atgagttacc cacaattgaa gttaaatgaa ccccttaatt ttaaggagat aatgaataat
     8881 taacgcaaca aaaccaacga tcatgaaaac aaaaagagaa ttgaattatc taggattaat
     8941 ctttatctta atagcaactc tatcgtctat ttatagttgt caaaagaacg attcatctga
     9001 aataactgca gatgtaataa ttagtagttc atcagttaat aatcctgtta ttattgatac
     9061 tatagttcag ca
//
LOCUS       DAOPHX010000103         9043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_9645_length_9043_cov_4.955051, whole genome shotgun sequence.
ACCESSION   DAOPHX010000103 DAOPHX010000000
VERSION     DAOPHX010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 9043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9043
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9645_length_9043_cov_4.955051"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1466)
                     /locus_tag="PK502_08115"
     CDS             complement(162..1466)
                     /locus_tag="PK502_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HPH00820.1"
                     /translation="MERDYQFETLQVHAGHRPDRDTLSRAVPIYQTTSYQFCSSDHGA
                     NLFELKEPGYVYTRMGNPTTDVLEKRIAALENGKAAVATSSGHAAQFLALSNILNAGD
                     NFVSSPFLYGGSYNQFNVALRRLGVDVKFSKSNNTEDFKALVDERTKALYVETIGNPS
                     FCIPDFENFAKLADRHGIPLVVDNTFGGGGWLCRPIDHGANVVVESLTKWVGGHGTSM
                     GGAVIDAGNFNWGNGNFPQFSEPSEGYHGLVFWKEFGENSPGGNTAFAVRARVEGLRD
                     FGATISPFNSFLMLQGIETLSIRMERHCQNTLLLAQWLEQHPKVESVCYPGLASNHYH
                     RLALKYLKHGFGGVLSFTIRGNTEQTKRFVDSLKLVSHLANVGDTRTLIIQPSATTHR
                     QLSPSEQLAAGVLPNQLRVSVGIENICDIKDDFEQAFEKLNS"
     regulatory      complement(1886..1992)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2063..2983)
                     /locus_tag="PK502_08120"
     CDS             complement(2063..2983)
                     /locus_tag="PK502_08120"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HPH00821.1"
                     /translation="MKKILLLLLAAIITLTSVGQGTVKKGKASTGPYPRGWVLVEKGG
                     KYGFISAEGDEIIKTQYTRISPFDEYLSGWALVEKDGFLGFISNSGDEVVKPQYTKIY
                     PFDEMQRGWAMVEISGLFGFISSEGREVVKPKYSKIYHFGEMYKNLAMVEKDGLVGFI
                     NLDGSEVLKPQYQKVHPFGEYKKDWAMVERDGFFGFINGEGKEVVAPKYSKVSPFGEY
                     QKNWALVEKDRYLGFIDMDGREVIKPQYSQILPFGQLQKDWAMVERDGLWGFISADGR
                     EVVKPKYEKINLDDVSGIKKTNEVVKDENS"
     gene            3387..4766
                     /locus_tag="PK502_08125"
     CDS             3387..4766
                     /locus_tag="PK502_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HPH00822.1"
                     /translation="MKRIKILHLAMQVAIAALLLSVSACEKEYPTINSNEQTAVVQDD
                     VQKATATFAENFESGTKTSYTGASVTLATGSWYLNDALLGNSSSDRKNGTKSVRIRNT
                     GKLTMQFDKTTGAGTVQIKHAKYGSDGSSTWELYISTNSGSTWSKVGSTVTTSSTTLT
                     AASFTVNVSGNVRFEVRKISGGSYRINIDDFTITDYGSTPPPSSDPTRDNNMALGNPS
                     GAVTSTAYANNYLMNKTQYSLSYNNSKLTCNWVSWHLSTAWLGSTPRQDDFRADATLP
                     SGWVQVGGSDYSGSGFDRGHMCPSADRTGSVTDNSATFLMTNMVPQAPNNNQITWANL
                     ENYCRTLVNAGNELYIISGPWGQGGTGSNGTKSTISSKNIVVPSYVWKIIVVLPVGSN
                     DVTRITSSTRVIAVWMPNTQTVSSQSWGYYRTTVDYLESKTGYNFLSNVSTTIQSAIE
                     SKVDNGPIN"
     gene            complement(4841..6115)
                     /gene="serS"
                     /locus_tag="PK502_08130"
     CDS             complement(4841..6115)
                     /gene="serS"
                     /locus_tag="PK502_08130"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPH00823.1"
                     /translation="MLTLKQIRENKEEVIRRLAVKHFDGAAIVGKIIELDDRRKAIQL
                     QLDTNLAEQNSLARQIGKLFAEGKSVEANDAKARTTNLKEQSKLLTQQLAGVEAEQRQ
                     LLLQIPNLPHDSVPKGVGADDNVIVRSGGEIPELPGDVLPHWDLAKKYDIIDFELGVK
                     ITGSGFPVYKGKGAKLQRALINFFLDCNTAAGYQEVEPPHMVNEDSAMGTGQLPDKDS
                     QMYHIGLDGFYMIPTAEVPLTNMFRDVILTSSMLPVKVTAYTPCFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQVQHPDKSYQSLEEMVLHVEGILKKLELPYRIVKLCGGDMSFTSA
                     LTYDFEVFSAAQKKWLEVSSVSNFESFQANRMMLRFKDDGDKRTQLAHTLNGSSLALP
                     RIVAALLENNQSPNGIRIPAAIKNYCGFDLIS"
     gene            complement(6205..6465)
                     /gene="rpmA"
                     /locus_tag="PK502_08135"
     CDS             complement(6205..6465)
                     /gene="rpmA"
                     /locus_tag="PK502_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPH00824.1"
                     /translation="MAHKKGVGSSRNGRESHSKRLGVKLFGGQVAKAGNIIVRQRGTK
                     HNPGQNVGMGRDHTLYALVDGEVSFRKKTDNRSYVSVIPKEA"
     gene            complement(6486..6797)
                     /gene="rplU"
                     /locus_tag="PK502_08140"
     CDS             complement(6486..6797)
                     /gene="rplU"
                     /locus_tag="PK502_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPH00825.1"
                     /translation="MYAIVDIVGQQFKVEKDQKLFVNRMAGETGTEVKFDKVLLVDND
                     GAVTVGKPQVENASVTAKILRHVRGDKVIVFKKKRRTGYKKKNGHRQNYTEIQISEIV
                     A"
     gene            complement(6925..7704)
                     /locus_tag="PK502_08145"
     CDS             complement(6925..7704)
                     /locus_tag="PK502_08145"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HPH00826.1"
                     /translation="MQIFKSTLLVFATILLWIIALASCIVFFAGDVVVWFLTFWWDKR
                     LWLLHRYSILWAMFYLKINPFWRVRFEGKENIRKEQVYVIVSNHQSAFDIILLYRLYT
                     HFKWVAKKELAKVPFIGWNLLLNNHIFIERGSAVSAKKMMEQGMKHLRMGSSLLIFPE
                     GTRSADGTIHRFKDGAFILAQKSEKPILPVVIDGSGEIMQNPLKLKLAQTLTIKILPE
                     ISYESFREFSPNELARHVQQLMEKEHMAIAPERYHGSMVNS"
     gene            7766..8107
                     /locus_tag="PK502_08150"
     CDS             7766..8107
                     /locus_tag="PK502_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00827.1"
                     /translation="MKPLKVAQPLASWLVRIIFAFYLFLIYLKGINPVQLNSLNFYLS
                     LTMLVFSVLLVVGGFLKTQTLTVVSAVIVGLILLYKALAPAPTAINAEFAQQALLISI
                     AFLFVCKGNQP"
     gene            8263..8802
                     /locus_tag="PK502_08155"
     CDS             8263..8802
                     /locus_tag="PK502_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00828.1"
                     /translation="MRYILLAVFCLISSFAYPQGVAQDTIILVSNKVIVGKVQNVSSS
                     KITLIKKDSQVPEEITRKQIHKIKYSNGRVEQFNSMAFSMVEESNFQAVIITDKPEDV
                     DGLYAYGKIESASGRTSKSAKAAEKNARIRLQRKAAAMGAMYVLITKSETRGGYKEVP
                     THFYEGIAYGIEPPKEQPK"
     gene            complement(8808..>9043)
                     /locus_tag="PK502_08160"
     CDS             complement(8808..>9043)
                     /locus_tag="PK502_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00829.1"
                     /translation="GAVTMRISVAHGHVESTAVDVVPDERLAAWCKTFLARLQGKRYD
                     IQSVADALVGAVEEYPGLEPGVTLDELLTCLIG"
BASE COUNT         2565 a         2200 c         1790 g         2488 t
ORIGIN      
        1 cgtaaatgca tggcatatcc aaacaacttt gtcggcattt ctaccaatgt cacctagtat
       61 gtgatacctt atgtttactt gctttaaaat ttgaccacac tctaagggta gagtatcaat
      121 tctaaagttc ccttcaatag ccgcgtacat tgcaaaaatt attagctgtt taacttctca
      181 aaagcctgtt cgaaatcgtc tttaatatca caaatgtttt cgattccaac cgacacccta
      241 agctggttag gcagcacacc tgcagctagc tgttctgaag gggaaagctg cctgtgggtt
      301 gtagccgatg gctgaatgat aagtgttcgg gtgtcgccaa cattagccaa gtggctaacc
      361 agcttaagac tatcaacaaa ccttttagtt tgttccgtat tccccctaat tgtaaaagat
      421 aatactccac caaatccgtg cttcaggtac tttaaggcaa ggcgatggta atgattcgat
      481 gctaaacccg ggtagcagac gctttccact ttggggtgct gttccagcca ctgtgcaagc
      541 agtagggtat tttggcagtg cctttccatg cgaatggaaa gagtttctat accttgaagc
      601 attagaaagg agttaaaagg gcttatggtt gctccgaaat cgcgtaaacc ttctactctt
      661 gccctcacag caaatgcagt gtttccgccg gggctattct cgccaaactc cttccaaaag
      721 accaaaccat ggtatccctc cgagggctct gaaaattgag gaaagttgcc gttgccccag
      781 ttgaaattgc ctgcatcaat caccgcacca cccatgctgg ttccatggcc tccaacccac
      841 ttggttaacg actcaaccac tacgtttgct ccatggtcta ttggccggca tagccatcct
      901 cccccaccaa atgtattatc aacaactagc ggaatcccat gcctgtctgc aagtttggca
      961 aaattttcga agtcgggaat acaaaagcta ggattcccaa ttgtttcaac atacagcgcc
     1021 ttggtacgct catcaactaa cgccttaaaa tcttccgtat tgtttgattt gctgaatttt
     1081 acatcaactc caagcctgcg tagggcaaca ttaaactggt tgtagctgcc tccgtataga
     1141 aatggagacg acacgaagtt atcgccagca tttaaaatat tactaagggc taaaaactga
     1201 gcagcatggc cagaagaagt agcaacagca gctttcccat tttcaagtgc agcaattcgc
     1261 ttttccagca catcggttgt tgggtttccc attcgggtat agacataacc gggctctttc
     1321 agctcaaaaa ggttagcccc atgatcggaa ctgcaaaact ggtatgacgt tgtttgatat
     1381 atgggcacgg ccctagacaa agtatccctg tcgggtcgat gaccggcatg cacttgcagg
     1441 gtttcaaatt gataatctcg ttccataagt agttgatttt taaaaaataa ttctacagta
     1501 ttgtaagctc aacacgtaaa ccttatgtct agctaggttt agtagctttc gatgagcctt
     1561 gtgagctcac tcctaaattc ttgaataaac aagaaaaaag gaatagacag atgttgtttt
     1621 gaattgtccg gacagcatat aggctaagcc atacaaagcc gactgggtga tacgtagcca
     1681 tatcaccgcc tgtaaccact tccaaatagt aacaggcata tccctttgag ggaatcaaaa
     1741 ttggattttt taccgcgctt agcggcgcat tcgcatggct ggggttaact tgctaggaga
     1801 gcatatcagc caacgcgaac tatttaagta gtccgaagag agaggaaacg ataaaatttt
     1861 aatggcacac atggcgttaa gttaagagtt ataattactc ctccacagga gtcaggtttt
     1921 agcaccttac aacttaaatg caggttgcta gagcttcaat gagcctgttc tctcagctct
     1981 tctctataat tccgatgctt ttagaagtaa aaacaagatg gtgcaaatat atttacgttg
     2041 ataaccaaat acaaatattc gctcagctat tttcgtcctt tacaacctcg ttggtttttt
     2101 tgatgcccga aacatcatca aggttgattt tctcgtactt aggcttcacc acctcccggc
     2161 catcggctga aatgaaaccc caaagcccat cacgctcaac catagcccaa tccttttgta
     2221 gctgaccaaa cggcagtatc tggctatact ggggcttaat cacctccctg ccatccatat
     2281 caataaaacc taagtagcgg tccttctcaa caagcgccca attcttttgg tactccccga
     2341 atggagaaac tttactgtac tttggagcaa caacctcttt cccctcccca tttataaagc
     2401 caaaaaatcc atcgcgctcc accatggccc aatccttttt atactcgcca aatggatgca
     2461 ctttttggta ttggggctta agcacctcgc ttccatctaa atttataaaa cctaccaacc
     2521 catccttctc caccatggcg aggttcttat acatttcccc aaaatggtaa atcttgctgt
     2581 actttggttt cacgacttcc ctgccttcgc ttgatatgaa cccaaacaac ccgctgattt
     2641 cgaccatagc ccagccacgc tgcatctcat caaaaggata aattttggtg tactggggtt
     2701 taacaacctc atcacccgag ttggagataa accctaaaaa gccatctttc tctaccagcg
     2761 cccatcccga caggtactcg tcgaagggag atattctggt gtactgggtt ttaataatct
     2821 catctccttc ggccgatata aacccgtact tgcctccctt ttcaaccagc acccatccac
     2881 gggggtatgg acctgttgaa gctttaccct ttttcacggt tccctgccca accgaggtaa
     2941 gggtgataat ggcagcaagc aaaaggagta gaattttttt catatcaaga ggtttatgcg
     3001 taaatatgaa tctcggtttc ccaatactta acagatacaa acctactgaa attttttatg
     3061 atacaccaaa gaggtaaggg taattctgcc ccacaacctt aaaaagtcat ggccaaaagt
     3121 taatgcggct aataaatttt accgcattcc cgggatgact cgttaaaaaa gtcaataatt
     3181 aaacggagaa cccgtaattc caactaaata gggcatttat aagacattgg aataaaggca
     3241 tgtagaaaat tcaaacggct aaacctaatt ttccagcatt aataacatgt tgttaacact
     3301 atccaaataa aataggtgca tttttgctac ttaagcaaac aaaactattt cataatttta
     3361 ttgcaccaac ctaaacctat ttaaccatga aacgaattaa aattttacat ctcgccatgc
     3421 aagttgcaat agctgcactg ctcctgtcgg ttagtgcctg cgagaaggag tatccgacca
     3481 ttaattctaa tgaacaaacg gctgttgtgc aggatgacgt tcagaaggct accgctactt
     3541 tcgccgaaaa ttttgaaagt ggaacaaaaa cctcctatac aggtgcatcg gttacccttg
     3601 ctacaggaag ctggtacctg aatgacgccc tgctaggaaa ttcatccagc gacaggaaga
     3661 acggaacaaa atctgtaagg attaggaaca ccggcaagct caccatgcag tttgataaaa
     3721 caactggagc cggaacggtg cagattaaac atgccaagta cggcagcgat ggcagcagca
     3781 cttgggagct ttacatctca accaacagcg gtagcacatg gagtaaggtg ggctcaacgg
     3841 taaccacctc atctaccact ctaactgctg cttcctttac agttaacgta tcgggaaatg
     3901 tacgtttcga agtaagaaaa atatccggcg gttcgtaccg aataaacatt gacgatttca
     3961 caattactga ctacggctct actcctcctc catcgtccga tcctacccgg gataacaaca
     4021 tggctttagg aaacccgtcg ggtgcagtaa ccagtaccgc ctacgccaac aactacctga
     4081 tgaacaaaac gcagtactcc ctatcataca acaacagcaa gctaacctgc aactgggtgt
     4141 cgtggcatct gagcacggca tggctgggta gtacccctcg tcaggatgat ttcagggccg
     4201 atgccaccct tccctcggga tgggttcagg tgggaggaag cgactacagc ggctccggct
     4261 ttgaccgcgg ccacatgtgc cccagcgctg accgcacagg gtcggttacc gacaactccg
     4321 ccaccttcct tatgaccaac atggtacccc aagccccaaa taacaaccag attacctggg
     4381 caaatttgga aaactactgc cgtacactgg ttaatgcagg taacgagctc tacatcatat
     4441 ccggcccctg gggtcagggt ggaacaggct ctaacggcac caagtcaacc atctcatcta
     4501 aaaatatcgt tgttccctca tacgtttgga aaattattgt tgtgctccct gttgggtcaa
     4561 atgatgttac gcgtatcacc tcatcaacaa gggttattgc tgtttggatg cccaacaccc
     4621 aaaccgtaag cagccagtca tggggctact accgcactac agttgactac cttgaaagta
     4681 aaacaggata caatttcctt tcgaatgtat caactacaat tcaaagcgcc atagagtcaa
     4741 aagttgacaa tggcccgata aactagagag gagtaattct cccttttaca aatgaaaaag
     4801 ccctgcaacc cagggctttt atcatatttc taaacaaacc ttagctaatc aaatcaaacc
     4861 cacagtaatt cttaattgca gcggggattc ttattccgtt aggcgattgg ttattttcca
     4921 gcagagctgc aacaattctg ggtagagcca acgagctgcc attgagggtg tgggctagct
     4981 gggttctttt atcaccatca tccttaaagc ggagcatcat gcgatttgcc tgaaacgatt
     5041 cgaagttgga aaccgagctc acctcaagcc actttttttg tgcggctgaa aacacctcaa
     5101 aatcataggt taacgccgaa gtaaagctca tatcaccccc gcaaagctta acaatacgat
     5161 atggaagctc cagcttttta agaattccct caacatgcaa aaccatttcc tctagcgact
     5221 ggtaagattt gtcgggatgc tgtacctgaa caatttctac cttgtcgaac tggtgcaggc
     5281 ggttaagccc cctaacatct tttccatagg agccggcctc acgtctgaag catggagtgt
     5341 aggctgttac cttaaccgga agcatggaag aagtcaggat aacatccctg aacatattgg
     5401 taagcggtac ctccgcagta ggaatcatgt agaaaccatc caggccgatg tggtacatct
     5461 ggctatcctt gtcgggtagc tggccagtgc ccatggccga atcctcgttg accatgtggg
     5521 gtggttcaac ttcctggtag cctgcagcgg tgttgcaatc aagaaaaaag ttgataagcg
     5581 cccgctgtag ctttgcccct ttccccttgt aaaccgggaa tccgcttccg gtgattttta
     5641 cacccagctc aaagtcaatt atgtcgtatt ttttggctaa atcccaatgt ggaagcacat
     5701 ctcccggtaa ttcgggtatt tcacctcccg aacgaacaat aacattatcg tcggcaccaa
     5761 ccccttttgg tacagaatcg tgcggcaaat taggtatctg aagtagaagc tgcctctgtt
     5821 cggcttccac cccggccagc tgctgcgtta gtagctttga ctgctcctta aggttggttg
     5881 ttctagcctt agcatcattt gcttcaacag atttgccctc ggcaaaaagt tttccgatct
     5941 gacgtgcaag gctattttgc tcggccaggt tggtgtcgag ttgtagctga attgccttcc
     6001 gtctatcatc cagctctata attttcccga cgattgcagc tccatcgaag tgctttacag
     6061 ccaaacgacg gataacttcc tccttattct cacgaatctg cttcagtgta agcatgacgg
     6121 catattttaa aataacaaaa aatcctgcac aacacattca gcattgagca ggagatcaaa
     6181 gttaaaagta tttttttacc tacctcaagc ttctttgggg ataaccgata cgtatgacct
     6241 gttgtcggtt tttttacgga agctcacctc gccatctaca agggcatata gggtatgatc
     6301 gcgtcccatc ccaacgtttt gaccgggatt gtgctttgtt ccacgctggc gtacaataat
     6361 attaccagcc ttagcaacct gccccccaaa aagtttaaca cccaggcgtt tactatgcga
     6421 ttccctgcca tttctcgaac taccaactcc ttttttgtgt gccatgattc gctaccttta
     6481 aaatgttaag ctacgatttc tgatatttga atctcggtgt aattctgacg atggccattt
     6541 ttcttcttat agcctgtgcg acgttttttc ttgaaaacga taaccttatc gcctcttaca
     6601 tggcgaagaa ttttcgctgt aaccgatgcg ttttctacct gaggctttcc aacggttaca
     6661 gccccatcgt tgtcaactag cagtacctta tcaaatttta cctcggtacc ggtttcccca
     6721 gccatgcggt tgacgaaaag tttctgatcc ttttcaacct taaactgctg gcctacaata
     6781 tcaacaattg catacattat tttcagaatg aaatgattaa catattttgg gcttgcaaaa
     6841 atataaaagt tttactttac gacaaatgtt ttgaaccatt ttttttaatt tgacttacct
     6901 acaacttcac caaagttaat atggctagct gttaaccata ctgccatgat acctttcggg
     6961 agcaatagcc atgtgctctt tttccattag ctgctggacg tgccttgcca gctcgttagg
     7021 ggaaaattcc ctgaaactct catatgaaat ctcgggtaga attttaatgg taagggtttg
     7081 cgctaacttt agcttcaggg ggttttgcat tatttcgcca gaaccatcta ttactacagg
     7141 taaaattggc ttttcagact tttgggcaag aataaaagcc ccgtccttaa accgatgaat
     7201 cgtaccatca gcagaacgtg tcccttcagg gaaaatcagc aaggagctgc ccattctcaa
     7261 gtgcttcatt ccctgctcca tcatcttttt agcgcttacc gcactacccc gctcaataaa
     7321 tatatggttg ttgagtagca agttccatcc aataaacgga actttagcca gctccttttt
     7381 ggcaacccac ttaaaatggg tgtaaagcct gtagagcaaa atgatatcaa atgcgctttg
     7441 atggtttgat acgataacgt aaacctgttc cttcctaatg ttttcctttc cctcaaacct
     7501 taccctccag aatgggttga ttttcaggta aaacatagcc catagaatgg agtagcgatg
     7561 taacagccac aaacgtttgt cccaccaaaa agttagaaac cataccacta catcacctgc
     7621 aaaaaaaact atgcaggagg ccagtgctat aatccacaac aaaatggtgg caaatactag
     7681 taatgtggat ttgaatattt gcattgagat cactagattt gaaaatattt tgcaaaggta
     7741 accttaacat ttaaatcatc cgaccatgaa accgttaaaa gttgcgcaac ccctagcaag
     7801 ctggctggtt cgtataattt tcgccttcta ccttttccta atttacctaa aaggaataaa
     7861 tccggtgcag ctgaatagcc tgaattttta cctatcgctt accatgcttg tgttttctgt
     7921 tctgctggta gtgggtggat tccttaagac ccaaaccctt acagtagttt cagccgtaat
     7981 agtagggctt atacttctat ataaagcact agcgcctgct cccacagcaa taaacgccga
     8041 atttgcccaa caggccttgc taatttcaat tgcattcctg tttgtatgca aggggaatca
     8101 accctaaccc aaaaatttaa aaatgttgaa gttgtaaagt aaaatcaaac tttcagtaat
     8161 tacctgttgt atcggtaaat tcaaacttca tttttagatt atttataaat gtatttttgc
     8221 aaatggcact acatttgtaa aattcttagc atgataagtt tcatgaggta catattgctt
     8281 gctgtttttt gcctgatttc atcatttgct tacccccagg gagttgctca ggataccatt
     8341 atacttgtaa gcaataaggt gattgttggt aaggtgcaaa atgtaagctc ttcaaaaatc
     8401 accttaatca aaaaggattc acaagttccg gaggaaatca cccgtaagca gattcacaaa
     8461 atcaagtata gcaacggccg tgtggagcag ttcaactcca tggccttttc tatggttgag
     8521 gaatccaact ttcaggctgt aattatcact gacaaacccg aggacgttga tggcttatat
     8581 gcctacggta aaattgaatc ggcatcggga cggacgagta aatcggcaaa agcggccgaa
     8641 aaaaatgccc gcattagact tcagcgtaaa gctgccgcaa tgggcgccat gtatgtgctg
     8701 attactaaaa gcgaaacacg cgggggctat aaagaagttc ccactcattt ctacgagggt
     8761 atagcatatg ggattgagcc tccaaaggag caacccaagt aatttactta gccaatcaaa
     8821 caggttaaaa gctcatccag ggttacaccc ggctccaagc ctgggtactc ttctactgct
     8881 cccacaaggg catctgctac tgactgaatg tcataccttt tcccttgcag cctggccaaa
     8941 aaggttttac accaggctgc cagccgctca tcaggaacaa catcaacagc ggtgctctca
     9001 acatgcccat gggctacact aatacgcatt gtaacagccc cca
//
LOCUS       DAOPHX010000104         8758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_10060_length_8758_cov_6.696886, whole genome shotgun sequence.
ACCESSION   DAOPHX010000104 DAOPHX010000000
VERSION     DAOPHX010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8758
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10060_length_8758_cov_6.696886"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PK502_08165"
     CDS             <1..221
                     /locus_tag="PK502_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00830.1"
                     /translation="FNSDGTGIDSEDYTFTWTLKGDKLAINQDGEEVTLTLTTKDGGK
                     MVGYLQETYTDEEEGDMTVKVIIEFAKV"
     gene            513..2783
                     /gene="rnr"
                     /locus_tag="PK502_08170"
     CDS             513..2783
                     /gene="rnr"
                     /locus_tag="PK502_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HPH00831.1"
                     /translation="MSKKGEKPSGRTPQKRGVTKRDLREGLMDVFIKNPNQSFNYKQL
                     STRLGLNNDNTRRMITEVLYELVGEERLVELSRGKFRLQEQTSLATGILVMSKDGSGE
                     VTTDDNREIFIPDFRLNHALHGDRVEISIYRHRRMGLLEGEVIRVIERAKRNFVGTIS
                     LLRNHAFVAPDNKLMPYDIFIPHVELRDIKNGQKVIVQITDWPPREKNPNGKIVEILG
                     NPGVHEVEMHAILAEFDLPYRFPEELEKLAEKIDDRISNADYASRRDFRDVPTFTIDP
                     FDAKDFDDALSVQILPSGNIEVGVHIADVTHFVKPNTPIDNEAIERGTSVYLVDRCVP
                     MLPEKLSNYICSLRPNEEKLCFSAVFELNDNAEVLSEWFGRTVIISKRRFTYEEAQAI
                     IETGEGDLHEQILLLHKLAQKLRRERFKNGAIAFERMEVKFELDAKGKPLGVYFKENK
                     ESNQLIEEFMLLANRKVAELVGKQKPGNERFPFVYRIHDKPNQEKLDNFRTFITRFGY
                     KLSGESSRQLGKSLNSLMDEVRGKPEQNLIETLALRSMAKARYSANNIGHYGLAFPFY
                     THFTSPIRRYPDMMVHRLLAHYLSNGKPKDKEQIELLCKHASEMEIRATEAERASIKY
                     KQVEFMQDKVGQVFMGVISGVTNFGIFVELSDSQCEGLVAMRDLDDDYYQFDEETYAL
                     VGKRTGRRFQLGDPVKVEVWRTNLAKKQLDFRLVDGEVHPGNSQLQVNRPQRAKKGSG
                     SKTHSMSRSTSKRRKK"
     gene            complement(2926..2999)
                     /locus_tag="PK502_08175"
     tRNA            complement(2926..2999)
                     /locus_tag="PK502_08175"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2963..2965),aa:Ile,seq:cat)
     gene            3085..3762
                     /locus_tag="PK502_08180"
     CDS             3085..3762
                     /locus_tag="PK502_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPH00832.1"
                     /translation="MAESSSTSFSIRNIRNIVFDLGGVLLDIDYSKAVMAFKKLGVAD
                     DDHLLSAGSMGIFNRFDCGQISPSAFHAEMCALVGMPLSYEQVVGAWNAMLLDWDVAR
                     LELVEALRQRYRVYLLSNTNSIHYSSYNQKLEAIAGGRSLSSYFDKAYYSFEVGLRKP
                     QREIFNLVVESSGLIPAETLFVDDTEENIMMASEVGMATRWIRPGEAALLSSILAGFL
                     SEPVGRS"
     gene            complement(3767..5005)
                     /locus_tag="PK502_08185"
     CDS             complement(3767..5005)
                     /locus_tag="PK502_08185"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPH00833.1"
                     /translation="MMKKIYLISFAAVAWLSACTTGEHKLATGPWLGVIQLDSTGGKL
                     DVPFNFELKNDSGTYSMTIRNADELINVDEVELVGDTLRARLPVFTSELVAYCGDSLK
                     GYYYPKGVQAGTRYKFYALAGQIDRFPWYSEPPAFNITGRWWFIENPGSPDSTVMVAE
                     LKQDGSRVTGTILSTTGDYRYFEGKVSGNRFYFSKNDGAQTIIVKGEISDENTMVNGV
                     ISGSPKWVSPWRAAKDPQAKLPEADKLVRVKKGFDTFEFAGKDISGNMVKSSDEKFRG
                     KVLAVLAGGSWCPNCLDEGRYYKQLYEKYRDQGFEVVSLCFEDKTFKESQPKIERFAK
                     SIGAAYTFLYVAPRGLEQRDSVLYAIEGQMAYPTSMILDRKGKIRRVETGFSGPGTGE
                     HFVTFAKETEELVKLLLEER"
     gene            5370..7805
                     /locus_tag="PK502_08190"
     CDS             5370..7805
                     /locus_tag="PK502_08190"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPH00834.1"
                     /translation="MSQVAKWLFRILAVAVFLLIAAAVVLPLVFKPQLKQLAKIEINK
                     NINATVEFDDFQVSLLRGFPNLFVGLKGVSIVGVERFAGDTLVSFGELGVRVNLWSII
                     DMEEIEVRSVYLSDAKVFARVTADGSKNWSIVKTSGLPQTEEEDTAAAKPTNIRIALT
                     RFKINNATFFYADDSLGMETTLRGVNLLISGNMVKRQTEIRLKSSADELDLRVKGIRY
                     IRDAEVRFNANMGVNLDNGTLSFRENELRINQLTLLFQGMMRMHKGSIGMDVSFSAPS
                     TDFKDLLSLIPAFYLKRYEDIQTSGQLKLYGSVNGVYNDSILPSASIEIAVANAMLKY
                     PKLPKSANGINFDAKVWFDGSNPDKSTVDVNRLQFSLGGNPFDAYLHVATPVSDMQVD
                     GKVVGKVNFATLADVIPLDSISVTGLLETNLKFGGRISHLKEKEYDKFNAEGSLNISN
                     FTLTGCDSTRQVKVGELIVNFSPKFVELQNLKSSLGSSDMQLSGRLENFIPFVFRNEA
                     LQGQLNLTSTLLDANELLSGFRVQAKQPVDSVVTHGVIKIPRNFNFTLSASVQHLLYG
                     RLDISNLKGNVEADSGIVNLYNLTMNMLGGDVALDGIYNPIDVSLPRVNLDLSIKDVD
                     IPLAFKSLTSLQRIVPTFKNLSGKLSGSISIASMLDSSMMPVPSSIIASGKLQSKSIG
                     FKESAVFGRLADVFKKEEFRSPYMSNVNVNFKVNESRIAIEPFSTSVGGAKMSVGGDM
                     GFDQSLNFKAKLAVPTELFDTAADALSKFSTKTNVRVSNLELTLKISGTSYKPLVKVD
                     WGDFTTNIDIPGL"
     gene            8269..>8758
                     /locus_tag="PK502_08195"
     CDS             8269..>8758
                     /locus_tag="PK502_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPH00835.1"
                     /translation="MRINKFLLVSVVPFLMLTSCQNWFNRHLIPGKAQREEVHQRFSE
                     RLKLVEAYDTSIQRIVSYDLPLKTREGFEFLYAYMPLSDLAMHSSSYVLEQVQLALEA
                     KDHFKWGRKVPSDIFLHFVLPYRVNNEYTDSARKVFFAELKPRLSGLSAYEAALEVNH
                     WCH"
BASE COUNT         2380 a         1942 c         2056 g         2380 t
ORIGIN      
        1 tgtttaacag cgatggaaca ggcatcgata gtgaagatta tacattcaca tggacgctta
       61 agggtgataa gcttgccata aatcaagatg gagaagaagt taccctaact cttacaacta
      121 aggatggcgg taagatggtt ggctacttac aggaaactta cactgatgaa gaggaagggg
      181 atatgacagt aaaagtaatt attgaatttg ccaaagttta agaattcctt caattttcta
      241 caaaggctat tcaacacctc tcaccaaaga ggtgtttttt attgatggtt ttttaaacag
      301 gtgttcattg ctgatgtatg aagtgaggta agcatgacaa ttatcctgcg ataaggctgt
      361 acgttcagct gaatactgaa tagaaaataa aacgtcctta ttttcacgga aagataatgc
      421 catcacggct ctttaaaatt ttaaaatcag gatagcgcaa aaacagttac atttgaaatt
      481 ctaatatcag cataaaactc aaacatttaa aggtgagcaa aaaaggcgaa aagccctcgg
      541 ggagaactcc ccagaaaaga ggggttacca aaagggacct tcgcgagggt ctgatggacg
      601 tattcatcaa aaaccctaat cagagtttta actacaagca gctatcaaca aggcttggct
      661 tgaacaacga caacacccgg aggatgataa ccgaggtgct ctacgagctt gttggcgagg
      721 aacggctggt tgagctttcg aggggcaagt tccgcctaca ggagcagaca agcctggcaa
      781 caggaattct ggtaatgagt aaggatggat cgggagaggt aaccaccgac gataaccgtg
      841 agatttttat tcctgatttt aggcttaacc acgccctgca tggcgatagg gtggagatta
      901 gcatttaccg ccaccgacga atggggttac ttgaaggcga ggttattagg gtgattgagc
      961 gtgccaagcg aaattttgtc ggaaccatat cgttgcttcg caaccacgcc ttcgtggctc
     1021 ccgacaataa gctgatgccc tatgatatct ttattcccca tgttgaactt agggatatca
     1081 aaaatggcca aaaggttatt gtgcaaatta ccgattggcc tccccgggaa aagaacccaa
     1141 atggaaaaat agttgaaatt cttggaaatc cgggtgtaca cgaggttgaa atgcacgcta
     1201 ttctggcaga gtttgacctg ccctacaggt tccccgagga actcgagaaa cttgccgaga
     1261 agattgacga ccgtatttcc aatgccgact atgccagcag gcgcgatttt agggatgtgc
     1321 caacattcac cattgacccg tttgacgcca aggattttga cgatgcgcta tcggttcaaa
     1381 ttttaccaag tggcaacatt gaggtgggtg tccatattgc cgatgttacc cacttcgtga
     1441 agccgaatac ccccatagac aacgaggcca ttgaacgggg aacttccgtt tacctggttg
     1501 accggtgcgt tcccatgctt cccgagaagc tttcaaacta catttgctcc cttcgcccaa
     1561 acgaggagaa actctgtttt tcggcagttt ttgagttaaa cgacaatgct gaagtcctta
     1621 gcgagtggtt tggacgcact gtaataatct ccaagcgacg atttacctac gaggaggctc
     1681 aagctattat cgaaaccggc gagggcgacc tccatgaaca aatacttctt ctccacaagc
     1741 tggcacagaa gcttcggcgt gaacgcttta aaaatggcgc cattgcattt gagcggatgg
     1801 aggtaaaatt cgaactcgac gccaagggta agccacttgg agtgtacttt aaggagaaca
     1861 aggaatcgaa ccagcttatt gaggagttca tgctgcttgc caaccgaaag gttgccgagc
     1921 tggttggcaa gcaaaaacct ggtaatgaac gatttccttt tgtttaccgt attcacgata
     1981 agcctaacca ggaaaaactc gataacttcc gcacatttat tacccgcttc gggtacaagc
     2041 tttcagggga gagcagcagg cagctcggta aatcacttaa cagcctgatg gacgaggtgc
     2101 gaggaaaacc cgagcaaaac cttattgaaa cacttgcact gcgttcaatg gcaaaagcgc
     2161 gttactctgc caacaacatc ggccactacg gactggcgtt ccctttctac acccatttta
     2221 cctcgcccat acgtcgttac cccgacatga tggttcacag gcttttagcg cattacctta
     2281 gcaacggtaa gcctaaggat aaggagcaaa ttgaattgct ttgcaagcat gcaagcgaaa
     2341 tggagattag ggctaccgaa gccgagcgcg cctctattaa gtacaagcag gttgagttca
     2401 tgcaggataa ggttggacag gtgtttatgg gtgttatttc aggggtaacc aactttggaa
     2461 tatttgttga gctaagcgac tcccagtgcg aaggcctcgt ggctatgaga gatctcgatg
     2521 acgactacta ccagtttgat gaggaaactt atgccttggt tggaaaaaga accggacgtc
     2581 gtttccagct gggcgacccg gtaaaagttg aggtttggcg aactaacctg gctaaaaagc
     2641 agctcgattt caggctggtt gatggggagg tacatcctgg caactcccag ctacaagtta
     2701 acaggccaca aagggctaaa aagggttcgg gtagtaaaac gcattccatg tcccgttcta
     2761 caagtaaaag aaggaagaag tagctggctg ctacttgtct gaggctaagc ccattcggat
     2821 gtagggtgtt ttatcagctt aaactccacc ttaattatag gttgttcaac acccttacag
     2881 cacagtcctt taaaaattga gaaggccacc ttgcggtggc ctgggtgggc ccagctgggc
     2941 tcgaaccagc gaccctctga ttatgagtca gatgctctaa cctgctgagc tatgggcccc
     3001 aaataatata acttctacag tttttatttg gagtgcaata ttacatattt gcgctgatat
     3061 ttcatcaaaa gggattaaaa aattatggcc gaaagtagct caacaagttt ttccatccgt
     3121 aatatcagga atattgtatt cgatttaggc ggtgttcttc tcgatataga ctacagcaaa
     3181 gcagttatgg cttttaagaa acttggtgtt gcggatgatg accacctgtt atctgccggc
     3241 tcgatgggta tttttaaccg gtttgactgt ggtcagattt ctccttcagc ttttcatgcg
     3301 gagatgtgtg ccctggttgg aatgcctctt tcctacgagc aggttgtagg tgcgtggaac
     3361 gccatgctgc ttgactggga cgtagccagg ctggagctgg ttgaggcgct tagacagcgc
     3421 tacagggttt acctgcttag caataccaac agcattcact acagcagtta caatcaaaaa
     3481 ctcgaagcca ttgctggagg tcgctcgcta agcagctact ttgacaaggc ttactattcc
     3541 tttgaagtgg gattgcgcaa accccagcgg gagatattca acctggtggt tgagagtagc
     3601 gggttgattc ctgccgaaac cctttttgtt gatgatactg aggaaaatat catgatggct
     3661 tcggaagtag gaatggcaac ccgctggatt cgacctggtg aggctgcatt gctatcgtcg
     3721 attttagctg gattcttgag cgaaccggta ggcaggagct gatggtctac cgctcttcca
     3781 gcagcaattt taccagctct tcagtttcct ttgcaaacgt aacaaagtgc tcgccggttc
     3841 ccgggcctga aaagcctgtt tcaaccctgc gaattttccc ttttcggtcc agaatcatgc
     3901 tggttgggta agccatttgc ccttcaatgg cgtacagcac cgagtcacgc tgctcgagcc
     3961 cacgaggggc aacgtacaga aaggtgtagg ctgcgccaat gctttttgca aaacgctcaa
     4021 tttttggttg tgattcctta aaagttttat cctcaaagca tagcgaaact acctcaaaac
     4081 cttggtcacg gtatttctcg taaagttgct tgtagtaacg tccctcgtca aggcagtttg
     4141 gacaccagct gcctccggcc agcaccgcca gcaccttacc tctaaacttt tcgtcggacg
     4201 atttcaccat gttgccgcta atatccttgc ctgcaaactc aaaggtgtca aagccctttt
     4261 ttacccgaac cagcttatcg gcttcgggca gtttagcctg cggatctttt gctgctctcc
     4321 acgggctaac ccattttgga ctacccgaaa ttaccccgtt aaccatggtg ttttcatcgg
     4381 atatttcacc ctttacaata atggtttggg cgccatcatt ttttgagaag tagaatcggt
     4441 taccggatac cttcccttca aaataccggt aatcgccggt agtgctaaga attgtaccag
     4501 tcacgcggct tccatcctgc tttaactcgg caaccatgac ggtggaatcg ggagagcccg
     4561 gattttcaat aaaccaccac cttccagtga tgttgaaggc tggaggttcg gaataccatg
     4621 ggaatcggtc tatttgaccg gcaagagcat agaatttgta tcgcgtcccg gcttgaacac
     4681 ctttaggata gtagtatccc tttagtgagt caccgcagta cgcaactagc tcgctggtga
     4741 aaacgggcaa cctggcccta agagtgtctc caaccagctc cacttcatca acgttgatca
     4801 gctcgtcggc attccgaatg gtcatggagt aggttccgga atcattttta agctcgaagt
     4861 tgaagggaac atccagcttg ccgcctgttg aatcgagctg gataaccccc agccaggggc
     4921 cggttgccaa cttgtgctcg cctgttgtac aggccgataa ccaggcaacg gcagcaaaag
     4981 aaatcaggta tatttttttc atcatcgtaa tggttaactt ttatttgatt tttaggcaat
     5041 gcctgagttt aatggttgcc ctaaacttaa agaaaattgc gaatgcatct attattttga
     5101 ccctgaaaaa aatctaaaat ttccttttac cctttgttct cgtcaagaat gatagcctgt
     5161 tgactctggt tctacatgcg aagcgaagct acaagtagca aacttaaact tttgattacg
     5221 ttaaaacatt gataggaaga acttttctat aattatttgt cgtgttgtgt aaccatattc
     5281 gggcttattt aaaagtttgt ctaaatttgt ttcaatcttc atcatcacta atcatctcca
     5341 tggcattaac ctaaaattta cccaaaaaga tgagccaagt tgcaaaatgg ctgttccgca
     5401 ttttagccgt agctgttttt cttttaattg cggcggctgt ggtgttacca ctggttttta
     5461 aacctcagct taagcagctg gccaaaatcg aaattaataa aaatattaac gctacagttg
     5521 aatttgacga ttttcaggtt tcgcttttaa gaggtttccc gaatttattt gtggggctta
     5581 aaggggtaag cattgttggt gttgaacgct ttgctggcga tacccttgta tcgttcggag
     5641 agcttggcgt acgggtgaac ctatggagca taatcgatat ggaggaaatt gaggtgaggt
     5701 cggtttacct aagtgatgcc aaggtattcg ccagggttac tgccgatggc agcaaaaatt
     5761 ggagcattgt caaaacttca ggcttgcctc aaaccgagga ggaggataca gctgccgcta
     5821 agccaaccaa catcaggatt gcactaaccc ggtttaaaat taacaacgcc acttttttct
     5881 atgctgatga ttcacttggc atggagacga cacttcgggg cgtgaacctc ttgatttcgg
     5941 gcaacatggt taaaaggcaa accgaaatca ggcttaaatc ctcggccgat gagttagatt
     6001 taagggtaaa aggcatacgc tacattcgtg atgctgaagt taggtttaac gctaacatgg
     6061 gggtcaacct cgataacggc accctatcgt tcagggagaa tgagctgcgt ataaatcagt
     6121 taaccttact ttttcagggc atgatgcgaa tgcacaaagg cagcattggt atggatgtta
     6181 gctttagcgc tccttcaacc gatttcaaag acctgctttc cctgataccg gcattctacc
     6241 taaagcgcta cgaggatatt cagacctccg gccagctgaa actttatgga agcgttaacg
     6301 gggtttacaa cgactccatt ttgcccagcg catcaatcga aattgcagtg gccaatgcca
     6361 tgctcaaata ccccaagcta ccaaaatcag ccaacgggat caactttgat gctaaggttt
     6421 ggttcgatgg ttcaaacccc gataaatcaa cagttgatgt aaacaggctt cagtttagcc
     6481 tgggcggcaa ccccttcgac gcctaccttc atgtggccac gcctgtaagc gacatgcagg
     6541 ttgatggcaa ggttgttggg aaggttaact ttgctacctt ggccgatgtt attcccctcg
     6601 acagcatttc cgttaccggc ctgctggaga ccaacctgaa gtttggagga cgtatttcac
     6661 acttgaagga aaaggagtac gacaagttca acgctgaagg ttcccttaac atttccaact
     6721 tcactcttac cggatgtgat tctacccggc aggttaaggt aggtgagcta atcgtcaact
     6781 tttcaccaaa attcgttgag ctgcaaaacc ttaaatcaag tctgggaagc agcgatatgc
     6841 agttaagcgg taggctggaa aattttatac cgttcgtttt taggaatgaa gccctgcaag
     6901 gtcaattgaa tctcacatca accctgttgg atgctaacga gctgctatcg ggatttagag
     6961 ttcaggctaa acagccggtt gattcggtcg ttacccatgg tgtaattaaa attcctcgca
     7021 actttaactt tactttgagc gcaagcgttc agcacctact ctacggcaga ctcgacattt
     7081 cgaacctgaa gggaaatgta gaggctgaca gcggcatagt caacctgtac aacctaacca
     7141 tgaacatgct tggaggtgat gtggctctgg atggtatcta taatcccatt gatgttagtc
     7201 tccccagagt taatctcgat ttgtccatta aggacgttga tatcccgttg gcatttaaat
     7261 cattaacctc actacagcgt attgttccaa ctttcaagaa cctttcgggt aaactatcgg
     7321 gaagtatcag cattgcctcc atgctcgact catccatgat gcccgtgccc agctccatta
     7381 tagccagcgg taagctccag tccaagagca taggctttaa ggagtctgca gttttcggga
     7441 ggttggcaga tgtttttaaa aaggaagaat tccgtagccc ttacatgagc aatgtaaatg
     7501 ttaactttaa ggttaacgaa agcagaatag ccattgaacc ctttagcacc agcgtgggtg
     7561 gtgctaaaat gagtgtaggc ggcgacatgg gtttcgacca gtctcttaac tttaaagcca
     7621 agctggcagt gcctaccgag ttgttcgaca ctgctgccga tgcgctgagc aagttttcta
     7681 ccaaaacaaa tgttagggtg tcaaacttgg agttgaccct taaaatttcc ggtacttcct
     7741 ataaacccct agttaaggtt gattggggcg attttactac caacattgac atccccgggt
     7801 tgtagttctg gattgagtac acgcagacaa aaaaagttta gaattgattt tacctggtgg
     7861 gcaatacaac actaaaccag aggcatgacc tgctttggtg atgagatgcc taaagaacct
     7921 gataagggtt ttggtttacc ctttgcaaac gtggggtgtt catagtcaac ctaaaaaaac
     7981 gcacatcatg ctttccgtaa aaagccctgc ataattttcc gtatgccagc caggttatat
     8041 taaatatggc tagatcaagg tctttttaac caccttattg tttttccagc tgcaaataga
     8101 ttatatttgc ttagccatta tagtttttcc ctttaattta caggataata gtgtttgcta
     8161 ggtattgtcc cagatttgaa tggagaggtg cttcattttt atttatatat tatccccgag
     8221 agtgaatatg tttcaatagc atgctgataa tcttaatatt ggtaaattat gcggattaat
     8281 aagtttctac tcgttagtgt tgtgccattt ttgatgctga cttcatgcca gaactggttc
     8341 aaccgccact taattccggg caaagctcaa agggaagaag ttcatcagcg cttcagcgaa
     8401 cggctcaagt tggttgaggc atacgataca tccattcaaa gaattgtttc ctacgactta
     8461 cccctgaaaa caagggaggg gtttgaattt ttgtatgcct acatgccgct cagcgattta
     8521 gccatgcaca gcagcagcta tgtgctggag caggtacagc tggcactgga ggctaaagac
     8581 cactttaaat ggggaaggaa agtaccttcc gacatttttc tgcattttgt gctgccctac
     8641 cgggtgaata acgagtacac cgattctgcc cgcaaggtgt tttttgccga gctaaaacca
     8701 aggttatccg ggttatccgc atacgaagct gccctggagg taaaccactg gtgccatg
//
LOCUS       DAOPHX010000105         8742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_10084_length_8742_cov_6.463681, whole genome shotgun sequence.
ACCESSION   DAOPHX010000105 DAOPHX010000000
VERSION     DAOPHX010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 8742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8742
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10084_length_8742_cov_6.463681"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(397..1692)
                     /locus_tag="PK502_08200"
     CDS             complement(397..1692)
                     /locus_tag="PK502_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPH00836.1"
                     /translation="MEVLQVEKIEEQIARKYDFLENLEAWLANQGFSENFAQLLKVGI
                     SVGVIILLALLTDYIAKRIFLSTMQRVAKRTTSEWDDVLVEKKFFQHLAHFAPAAVVY
                     FTVGIALYDYTPKITLIIQALAKIYMVIVGVLVFNSFFDSVNTIYQKLPFAKSRPIKG
                     YLQVVKIILYVFAGIIIIGIIINRNPGTILVGLGASTAVLMLVFKDTIVGLVASIQLS
                     ANNMLKIGDWIEMPGRNIDGTVTDVSLTTIKVRNFDNTISTVPPYALVTESFRNWRGM
                     EESDGRRMIRSIFIDAKTIKFCSPQLLEKLKEVSYCHDWVVEQDEDELKRANEDIAML
                     NKGIPTNLYLYRRYLEGYIKSHPEVNQELPLVVRLKQQVEYGIPVEMYCFLKDKSWVP
                     YETTQSDIVDHALAALPVFELRTFQRMTSDDIRPTAGRS"
     gene            complement(1849..2220)
                     /locus_tag="PK502_08205"
     CDS             complement(1849..2220)
                     /locus_tag="PK502_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HPH00837.1"
                     /translation="MSMEITGKLLQKLNRQTGEGKNGAWVKQEFIIETLEQYPRKICI
                     AAWGTRVDDLDRLQPGNILKISVNVESREFNGRWYTDVKAWRIEKQDDEAPQSDGGKP
                     GKEDMNLPPESSKEYFDDLPF"
     gene            complement(2292..3008)
                     /locus_tag="PK502_08210"
     CDS             complement(2292..3008)
                     /locus_tag="PK502_08210"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPH00838.1"
                     /translation="MKLNKLLAAAILFPAVVSCQSTNGKIGKVAMKNDTDSISYAIGV
                     MYAQNLKQSQLETVNYQAVARAIQDIYSGDSANVKMSDNQAMAYINEYFRKLDQKIAE
                     KNLAESTKFLDENKTKEGVVVDSTGLQYKIITEGNGPIPTADAVVKVHYKGTLVNGKQ
                     FDSSYENGEPAQFKLNQVIRGWSIGLQKMKVGSKFMLYIPADLAYGVNVRPGGPIGPN
                     QALIFEVELLDIVKEEPAKK"
     gene            complement(3285..3962)
                     /gene="mnmD"
                     /locus_tag="PK502_08215"
     CDS             complement(3285..3962)
                     /gene="mnmD"
                     /locus_tag="PK502_08215"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HPH00839.1"
                     /translation="MTKLISAITSDGSSTIYSELHGAYYHSLNGALTESKHIFVEAGY
                     KEAIRVRGDVSILEVGFGTGLNAALTAAEASYSGIKISYMAIEKYPVTDEVIKTLNYG
                     EMLGTEAATLWGKIIRAPWSCEVQISSLFSIAKICTDFILWPAPQNRYNLIYFDAFAP
                     DDQPEMWTQPQFLKLFNSLLEGGILVTFSSKGSVKEALRQSGFTVHRIPGPPGKRHIL
                     RACKSAT"
     gene            4180..6294
                     /locus_tag="PK502_08220"
     CDS             4180..6294
                     /locus_tag="PK502_08220"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPH00840.1"
                     /translation="MAARLWNVKFYLLGLVLLNSCSLAPAPPKAEVHPYQMVTCGDTR
                     IDPYFWLCYRDSQKVLDYLLAENAYTEAMMKSTMPLQEKLFNELKSRKKVEDVTVPAF
                     MNGYFYYTRFEEGKEYPINCRRKGNMDAPEEVILDENEVAAGQRYCQVASLDISPNNQ
                     ILAFGVDTLGKRLFTIYFKNLETGKLLPIKIDNTTGTIAWASDSQTLFYTQKDQTLRP
                     SRIYRHKISKNANPKDELVYYEADSTFSTHVVNSKSLEYIFIYSSSGSCSEYRYLKTS
                     TPEGKFKVFQPRMPGVEYTVEHSGNFFYIMTSWNAENNRIMETPLTSTRKESWRELVP
                     NRSDVLVMNVDIFKDFLVATERKDALIQVKVISLRDKSQHYIDFGEEVYTALPSVNSE
                     FNTSSFVYFYTSLTTPGVTCEYNMQTHQKKILKQNEVLGGYNPDNYETKRLWATAADG
                     VNIPISVVYRKGIKLDGKNPLLIYGYGAYGYPVNPTFDPNLLSLLDRGFVYAIAHVRG
                     GQELGRKWYEDGRKLCKMNTFTDFIACTECLVEQKYTSPDRIVAQGASAGGLLMGVIA
                     NLRPDLYRAIVAEVPFVDVLTTMLNPNLPLTTGEYDEWGNPNDSIVYHYIKSYSPYDG
                     VRQQDYPSMLVLASYYDSQVQYFEPAKWVAKLRASKTDNNLLLLKTDMESGHAGSLGR
                     YKALKDEAFVYAFILHAIGIED"
     gene            complement(6318..8675)
                     /locus_tag="PK502_08225"
     CDS             complement(6318..8675)
                     /locus_tag="PK502_08225"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPH00841.1"
                     /translation="MEKPDNTDSSGIEDKYQALLGLIPDMIFIFNSDVRYVGYHGGKD
                     APTYVPPEMFLGKRIEEVLPEPVASNARVVVEEVLKTGKMQTYSYQLTIDNKPQFFES
                     RMVRYGTSKVLAIVRDVTSRTNAENALKESEEKYYNLYNLMRLMADTIPDMVWAKDLN
                     QNYIFANMAFCSTILNARDTDEPVGKNDMFFAKRERESHPENPEWHTFGELCMNSDLD
                     TLRELKEMQFDEYGNVKGKFIFLDVHKSPLYDKNGKLIGVVGSGRDITEKKKMEEHLA
                     LSEKTYRGIINSISEAVYVQDADGTFLDVNKACEKYFGYQREYFIGKKPDDLSAPGKN
                     DMEMVAACIQKAYNGQPQIFEFWGRRSDGFIFPKEVSLTPGEYFGRKVVIAVSRDITE
                     QKNWEAELIAAREKAEESDRLKSAFLANMSHEIRTPMNGILGFAQLLKNPRLSEEEVR
                     EYLDVIEQSGQRMLDIINNLINISKIESGEVQVNLSQCDVNEQLQYALNFFKPDADSK
                     GLKFFLEYQLPDEQATVTTDPLKLQVILSNLVKNAIKYCDNGSVAFGCKLKEEYLEFY
                     VRDTGIGIPPLTIGKIFERFVQANTNYSGRYEGAGLGLAIAKSYVELLGGSIWVESEV
                     GKGSTFHFTIPVRPAARQQQSYSNALNQDKNDSVSQPFSKKLKILIAEDDAPSGMLLR
                     NQLKDIAGEIISARNGADAVSMFLSNPDTDLILMDIKMPEMDGYEATKKIREVSSNVV
                     IIAETAYAMTNDREKALSVGCNDYISKPINREKLLELVKKHFELD"
BASE COUNT         2388 a         1994 c         1860 g         2500 t
ORIGIN      
        1 cttgcggtgt agtaaaatat tctttaaaac agggttagat gctacggctg gtaatagcta
       61 caatggagag tggcgttggt ttcatacagt ataagcgcca atgtaggaca gctatcacaa
      121 aaacggataa acaaccggat gttatttgtg ggttcggcga aatcttccta tgagtgtcag
      181 cctacggcaa gccttttggg gttcatccga atgtaagagg tagaaaaata ctaaatcgct
      241 gcttactgtc atcaaaatat aatatttgct tgtagaaaaa gcgcagcaat ctacctctgg
      301 cattgcaacc atagcaatca aacgggtaag gctgataagg agaggttcaa aatttgctcc
      361 tataacagga atagaaaagc ttagctatag ccttgattag ctcctaccag cagtagggcg
      421 gatgtcatcg ctcgtcatgc gctgaaaagt tcggagctca aaaacaggta gtgctgccag
      481 ggcgtggtca acaatatcgg actgggtagt ttcgtaggga acccaactct tatccttcag
      541 aaaacagtac atctcaacag ggataccgta ctccacctgt tgctttaggc gaacaaccag
      601 tggtagctct tggttgactt cgggatgtga ttttatgtaa ccctccaagt acctacgata
      661 aaggtacagg tttgttggga tacctttatt tagcatggca atatcttcat tagctctttt
      721 aagctcatct tcatcctgct caacaaccca atcatgacag tagctaactt ctttaagctt
      781 ttcaagcagc tggggagaac aaaacttaat ggttttggca tcaataaaaa tggaacgaat
      841 catccttcga ccatccgatt cctccatacc cctccagttt cggaaactct cggtaacaag
      901 cgcatagggt ggaacagttg aaatggtgtt gtcaaagttt cttaccttta tggtggttag
      961 actaacgtct gtaactgttc catcaatgtt gcggcctggc atttcaatcc aatcaccaat
     1021 tttaagcatg ttgttggccg aaagttgtat ggaagctaca agcccaacta tagtatcctt
     1081 aaacaccagc atcagcacag ccgttgaggc acctaatcct accagaatag ttccggggtt
     1141 gcggtttatg atgataccta taatgattat tccggcaaaa acgtatagaa taattttcac
     1201 aacctgtagg tagcccttaa tggggcgact tttagcaaaa ggcaacttct ggtatatggt
     1261 gtttaccgaa tcaaaaaatg agttaaaaac cagaacgcca actataacca tgtaaatttt
     1321 agctagcgcc tgaataatca gggtgatttt tggagtgtaa tcgtagaggg caattccaac
     1381 ggtaaagtaa actacagcag ccggagcaaa atgtgccagg tgctggaaaa actttttctc
     1441 aaccagcacg tcgtcccact cgctggtggt gcgcttggca acccgctgca tggtggacaa
     1501 aaagatacgc ttggctatgt agtcggtgag tagagccaat agaatgatta ccccaaccga
     1561 aatgccaact tttagcagct gggcaaagtt ttcactaaat ccttggtttg caagccatgc
     1621 ctccaggttt tcgagaaaat cgtactttcg ggcaatttgc tcctcaattt tttctacttg
     1681 taacacttcc acctagtaag ttgatttaat ggtttactac ttgcttgttg tgtagctggt
     1741 tataagatat ttttaaatga agatgttggt taggctatag aaatctaagg caatgcttta
     1801 ttcgcacgtt atgaacttca gctgcaagcc aaaccagcat gcagcttgct aaaaaggcaa
     1861 atcgtcgaaa tattcttttg atgattcggg aggtaggttc atgtcctctt taccgggttt
     1921 tcccccatcc gattgaggtg cttcatcgtc ctgtttttct atgcgccaag ccttcacatc
     1981 ggtgtaccat cgcccgttaa actccctcga ctcaacatta acagaaattt taaggatgtt
     2041 tccaggctgt agccggtcaa gatcatcaac ccgggttccc catgcggcta tacaaatctt
     2101 tcgggggtac tgctcgaggg tttcaataat gaactcctgc tttacccaag ctccattttt
     2161 accttcaccg gtttgccggt tcagcttttg aaggagtttc cctgttatct ccatgctcat
     2221 gttacttaaa atttagaaaa acagccggat gaaccacacc cggctggtaa gctgcgaggt
     2281 taaacaatat actatttttt agcgggttct tccttaacaa tgtcaaggag ctcaacttca
     2341 aaaataagcg cctggttggg tccaataggt ccaccgggac gaacattaac accataggcc
     2401 aaatcggctg ggatgtagag cataaacttg gaacccacct tcattttctg aaggcctatg
     2461 ctccagcctc taattacctg gttaagtttg aactgggcgg gttcaccatt ttcgtatgat
     2521 gaatcaaact gcttcccgtt aacaagagtg cccttgtagt gaactttaac cacggcatcg
     2581 gcagtaggaa tggggccatt cccctcggta ataattttat actgaagtcc ggtagaatca
     2641 acaactacgc cctcttttgt tttgttttca tctagaaatt tggtgctttc agccaggttt
     2701 ttctcggcaa ttttctggtc aagcttcctg aagtactcgt taatgtatgc catagcttga
     2761 ttgtcgctca tcttgacatt ggctgagtcg ccactgtaaa tatcctgaat ggcgcgtgcc
     2821 acagcttgat agttgacggt ttcgagctga ctttgcttta aattctgcgc atacatcaca
     2881 ccaatagcat agctaatact atcggtatca ttcttcatgg ccacctttcc aatcttacca
     2941 tttgtacttt ggcacgaaac aacggctgga aaaagaattg ccgcagcaag taacttgttt
     3001 aatttcatct gattaatttt atttgattag ttgatattca gattgacaca aaatttatct
     3061 catccagata acttctagca aactttaaca accgcctcgg gttcaacagc aacgcttctc
     3121 ttccactaat cctagaattg cctaaatacc caagtttttt tttaaaatta ctcaacaata
     3181 atattccctg cgaaggtaat acatatttaa actgaattaa aattttaggg taaaaatttc
     3241 ttggtgctgg tttttgaaac gaaaaattgt aacatttaac aatactacgt ggcagattta
     3301 caagccctga gtatatgcct tttaccggga ggtccgggaa tacgatgaac tgtaaagccc
     3361 gattgcctca gggcctcctt cacgctgccc tttgatgaaa aagtaaccag tattccacct
     3421 tctagcaagg agttaaagag tttaagaaac tgtggttgag tccacatttc gggttggtcg
     3481 tcgggggcaa acgcatcgaa gtatatcagg ttgtaccggt tttgaggtgc aggccataaa
     3541 ataaagtcgg tgcaaatttt agcgatggag aacagggagc taatctgaac ctcacagctc
     3601 caaggcgccc ggattatttt tccccagagg gtagctgcct cggtgccaag catctcgccg
     3661 tagttaaggg tttttataac ctcgtctgtt accggatact tttcaatagc catgtagctg
     3721 attttgatgc ccgaatagct cgcttcagct gcagtgaggg cagcgttaag cccggttcca
     3781 aacccgacct cgagtatgga aacatcacct cgtaccctaa tagcctcctt gtaacctgcc
     3841 tcaacaaaaa tatgcttcga ttccgtaagg gcgccgttaa gtgaatggta gtatgcgcca
     3901 tgaagttcgg agtagatcgt gctcgagcca tccgaagtaa tggctgaaat aagctttgtc
     3961 atggatttca acacaataga tgctcaaagt taggggaatt ttaaatattg aggtaagtgc
     4021 atataaggag aagtaaccag taggagctga ccatctgaat ggatagtttt ctttacaata
     4081 tctgcaacgt attgtacgtt aaaatgttac gaataaatgt ggagtagatt tgcaggtaac
     4141 cggccaaaat cataattttg gttttattta tcgaaaatta tggcagctcg cttatggaac
     4201 gtaaagttct acctgttggg attggtttta ctgaatagtt gctccctggc acccgctcca
     4261 cccaaagctg aagtgcatcc ctaccaaatg gttacttgtg gcgatacccg tattgatccc
     4321 tacttctggt tgtgctatcg cgattcgcaa aaggtgctgg actacctgct agctgaaaat
     4381 gcctacacgg aggccatgat gaagtccacc atgccacttc aggaaaaact attcaacgag
     4441 ttgaaatcgc gcaaaaaggt agaggatgtt acagttcctg cgtttatgaa cgggtatttt
     4501 tactacacgc gatttgagga agggaaagag taccccatta actgccgcag aaaaggtaac
     4561 atggatgccc ccgaggaggt aattctcgac gagaatgaag ttgctgccgg tcagcggtac
     4621 tgccaggttg ccagcttgga tatttcgccc aacaaccaaa ttcttgcctt cggggttgat
     4681 accctaggca aaagattgtt caccatttac tttaaaaact tggaaaccgg caagttgctc
     4741 ccaatcaaga ttgataatac taccggaaca attgcctggg caagcgatag ccaaacgctc
     4801 ttttacacgc agaaagacca aaccctccgc ccgtcaagga tttatcgcca taaaatttcc
     4861 aaaaatgcca atccaaagga tgagctggtt tactatgaag ccgattcaac atttagcact
     4921 catgttgtta actccaaatc gttggaatac atctttatat acagcagcag cggttcatgc
     4981 tccgagtacc gctacctaaa gaccagcaca cccgaaggta aatttaaggt atttcaaccc
     5041 cgtatgcccg gtgtggaata caccgtagag cactcgggta actttttcta cattatgacc
     5101 agctggaatg ccgaaaacaa taggattatg gaaacgcctc tgacatctac ccgaaaggaa
     5161 agctggaggg aacttgtccc gaacaggagt gatgtgctgg tcatgaatgt ggacatcttt
     5221 aaggattttc ttgttgctac agagcgaaaa gacgctttaa tccaggttaa ggtgatatca
     5281 ctcagggata aatcgcagca ctacatcgat tttggcgagg aggtatatac tgctttacca
     5341 tcggtaaact ccgagtttaa cacttcatca tttgtttact tttacacctc gctcaccaca
     5401 ccgggagtaa cctgcgagta caacatgcaa acccatcaga aaaaaatact caagcaaaat
     5461 gaggttcttg gtggctataa ccctgataac tacgaaacaa agcgtttatg ggcaaccgca
     5521 gccgatgggg ttaacattcc tatttcggtg gtttaccgca agggcattaa gctagacgga
     5581 aaaaatccgt tactcatata cggttatggg gcttacggct acccggtaaa tcccaccttc
     5641 gatcctaacc tgctaagcct gctcgatagg ggctttgtgt atgcaattgc acacgtcagg
     5701 ggaggacaag agctgggtcg caaatggtat gaagatggca ggaagttatg caaaatgaac
     5761 acatttaccg acttcattgc ctgcaccgaa tgccttgttg agcagaaata cacctcgcct
     5821 gataggatag tagcacaggg cgcaagcgcc ggaggcctcc tgatgggggt tattgccaac
     5881 cttcgtcccg atttatacag ggcaattgta gctgaagtcc cctttgttga cgtgcttacc
     5941 accatgctta atcctaacct accgctcaca actggagaat atgatgaatg gggcaaccca
     6001 aacgattcca tagtttacca ctatataaaa tcatactccc cttacgatgg cgttaggcag
     6061 caggattacc ctagcatgct tgttctagcc agctactatg attctcaagt tcagtatttt
     6121 gaacccgcca agtgggtagc caagttgaga gccagcaaaa cagataacaa cctcctactt
     6181 ctaaaaacag atatggaatc gggtcatgcg ggtagtttgg gcaggtacaa agctctaaaa
     6241 gatgaagctt ttgtgtacgc atttatactt cacgcaatag gaattgagga ctagaatcac
     6301 tctaaagacg cttccagcta gtcaagttca aagtgttttt ttacaagctc cagtaatttt
     6361 tcccggttta tgggtttgga aatgtaatca ttacatccca cgctaagggc tttctccctg
     6421 tcgtttgtca tggcataggc cgtttcggca ataataacca cgttggagct tacctctcta
     6481 atttttttgg ttgcttcata gccatccatt tctggcattt taatatccat aagtattaga
     6541 tcggtatcgg ggttactaag aaacatgctc acagcatcgg caccattcct ggccgatatt
     6601 atttcacctg caatatcctt cagctggttt ctaagtagca ttcccgaagg ggcatcatcc
     6661 tcggctataa gaattttcaa ctttttacta aagggttgac ttaccgaatc gtttttatcc
     6721 tgatttaaag cattagaata agattgctgc tgacgagcag caggccgaac cggtatggta
     6781 aaatggaaag ttgaaccttt ccctacttcg ctttccaccc aaatactccc cccaaggagc
     6841 tctacatacg attttgcaat ggcgagcccc aatccagcac cctcgtacct accagagtag
     6901 ttggtgttag cctgaacgaa ccgctcaaat atctttccga tagtcagagg gggaattccg
     6961 atacctgtgt ccctcacgta aaattccagg tactcctcct ttaatttgca accaaacgct
     7021 acacttccgt tatcgcaata ctttatggcg tttttgacta ggttcgatag aattacctgc
     7081 aactttaacg gatcggtagt aacagtggct tgctcatcag gtagctggta ttccagaaaa
     7141 aattttagcc ccttactatc ggcgtcgggc ttaaaaaagt tcaacgcata ctgcagctgc
     7201 tcgtttacgt cgcattggct taggttcacc tgcacttcac ccgactcaat tttagaaatg
     7261 ttgataaggt tgttgattat atcgagcatt cgctgaccgc tctgctcaat aacgtcaagg
     7321 tattccctta cctcctcttc cgagagtctc gggtttttta gtagctgggc aaaaccaagt
     7381 attccgttca tcggggttcg aatttcgtgg ctcatgttgg ctaaaaatgc cgattttagc
     7441 ctatcgctct cctccgcctt ctcacgtgcg gctattagct cagcctccca attcttttgc
     7501 tcggtgatgt ccctggaaac agctatcacc acttttctac cgaagtactc gccgggagtt
     7561 aagcttacct ctttagggaa aatgaaacca tcgctacgac gtccccagaa ttcaaagatt
     7621 tggggttgtc cgttgtacgc cttttggata caagctgcca ccatttccat gtcgttttta
     7681 cccggggcag acagatcatc aggctttttc ccgataaagt actcccgctg gtatccaaag
     7741 tatttttcgc aggccttatt tacatccagg aaggtgccat cggcatcctg aacataaacc
     7801 gcttcggata tgctgttgat aatgccgcgg taggtttttt cagatagagc caggtgttcc
     7861 tccattttct ttttctcggt tatatccctt ccgcttccta ctacgcctat tagttttcca
     7921 ttcttatcgt acaggggtga cttatgcaca tcaagaaaga tgaactttcc cttaacattt
     7981 ccatactcat caaactgcat ctccttcagc tcacgaaggg tatccaggtc agagttcata
     8041 cataattcac cgaatgtgtg ccactcagga ttttcaggat gggattccct ttcccttttg
     8101 gcaaaaaaca tgtcattttt gccaactggt tcatcggtat ccctggcgtt tagtatggtg
     8161 ctgcagaatg ccatgttggc aaaaatgtag ttttgattta agtcctttgc ccacaccatg
     8221 tcgggaatag tgtcggccat taacctcatc aggttgtaaa ggttgtagta cttttcctcg
     8281 ctctccttaa gggcattctc cgcatttgta cgactggtta catcccggac aattgccaaa
     8341 accttggagg tgccgtatct aaccatccgg ctttcaaaga attgtggctt gttatcaatg
     8401 gtaagctggt agctgtaggt ttgcatttta ccggttttca gcacctcctc aaccacaact
     8461 cgggcatttg aagcaacagg ctcgggcagc acttcctcta tgcgtttgcc aagaaacatt
     8521 tcgggtggaa cataggtagg tgcatccttg ccgccatggt agccgacata ccgtacatcg
     8581 gagttaaaaa taaaaatcat atcggggatt aagccaagca gcgcctggta tttatcctca
     8641 ataccgctac tgtcagtatt gtctggtttt tccattttga gcaaagtaat gttattaatt
     8701 tttacaattt aggtaaacga taggagaaag tcaacatttt tt
//
LOCUS       DAOPHX010000106         8726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_10116_length_8726_cov_5.014301, whole genome shotgun sequence.
ACCESSION   DAOPHX010000106 DAOPHX010000000
VERSION     DAOPHX010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10116_length_8726_cov_5.014301"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PK502_08230"
     CDS             <1..871
                     /locus_tag="PK502_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPH00842.1"
                     /translation="LTDQNGLDSLQIAPGYYNFSVRASGYNMSSPGYFFIEPSDTLIE
                     VFMQPATTHSLTLQVIDEKGTSLPGAQVTTLNHGAKLTNDNGEAFFSNVPEGEISCMV
                     YKENYINNVAGVNLTSDSTVTVLLYANRYTATFFVTSKNVPVYNAQVIVEGIDTAYTS
                     ATGYANINLIPFGSNIRYSVKLNGYNTYLGTFNLEDRNIVVNVPLTLTTIEPTKTNSF
                     AIYPNPSNGNFSINLEGSFSVAIYNLAGYKVYLSNHAENSLQVDIHSLPAGIYIVRVT
                     GKNFSTVAKIIKK"
     gene            1354..2673
                     /locus_tag="PK502_08235"
     CDS             1354..2673
                     /locus_tag="PK502_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPH00843.1"
                     /translation="MKETPIPREIVKQKIAESNLPRVGRGSIREIVHLVNEIEKASGQ
                     KFIRMEIGDPGLQPAEIGIEAEISALRSGVASKYPLVDGIPPLKKEISRFVKNFINID
                     VDERGCIPTVGSMQGGMAAFLMTTRCNTRKNTVLFIDPGFPVQKQQLKVLGIDYETFD
                     VYNFRGDRLHDKLESYLKKGNISTILYSNPNNPSWICFTEKELRIIGELATAYDAIVV
                     EDLAYLGMDFRKDLSKPGQPPYQPSVANYTNNWLMLISSSKIFSYAGQRIGCIVVSNE
                     LYNRHFPDLKRFFSSDEFGYAMIYGAVYPLSSGVCHSAQYGFAAMLKAANDGVYNFVN
                     DVREYGEKAKVMKKLFTDNGFKIVYDRDEGEPLADGFYFTISYPGLTGEELIEEMLYY
                     GISAISLLITGSERSEGLRACVSQVQRSQFSDLEYRLKKFHEDHPTK"
     gene            2724..2960
                     /locus_tag="PK502_08240"
     CDS             2724..2960
                     /locus_tag="PK502_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPH00844.1"
                     /translation="MAKIRGAIVVDVERCKGCSLCVVACPSSVIALAKEVNGKGYNYA
                     YMVNPDACTGCVNCAIVCPDTVISVYKVKADAVA"
     gene            3002..4087
                     /locus_tag="PK502_08245"
     CDS             3002..4087
                     /locus_tag="PK502_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPH00845.1"
                     /translation="MAELKLMKGNEAIAEAAIRCGCDGYFGYPITPQSEIMETLMARQ
                     PWDETGMVVLQAESEVASINMVYGGAACGKRAMTSSSSPGISLMQEGITYIAGSELPC
                     LIVNVVRGGPGLGTIQPAQSDYFQAVKGGGHGDYKLIVLAPASVQEMYDFVDLAFELA
                     FKYRNPAMILSDGVIGQMMEKVQIGDYKPRLTDEEVVKRYGSWATTGKPSTRERNIIT
                     SLDLDANSQELFNLKLLKKYAEIEKNEIRFEEISCTDAEYLLVAYGSSSRICQKTVDL
                     AREKGIKVGLLRPITLYPYPYGAIQKLVPQLKGILAVEMSAGQMVEDVRLAVNGKVPV
                     LHYGRMGGVIPTPNEVLKVLEDKLIKG"
     gene            4092..4853
                     /locus_tag="PK502_08250"
     CDS             4092..4853
                     /locus_tag="PK502_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPH00846.1"
                     /translation="MDVKDIIKEENLVYSKSKVLTDTIMHYCPGCTHGVVHKVIAEVI
                     EELGIQDKTIGVSPVGCAEFAYNYLDIDWQEAAHGRAPALATAINRLYPDKYVFTYQG
                     DGDLASIGTAEIIHACNRGENIVVIFINNGIYGMTGGQMAPTTLIDMPTSTTPYGRKV
                     DLNGYPLKITDLVAQLPGTCFVTRHSAHTPAAVRKLKKAVTKAFQNTRRKKGVSFIEV
                     VSTCNSGWKLSPVESNNWMVEHMFPYYPLGDMKDE"
     gene            4881..5426
                     /locus_tag="PK502_08255"
     CDS             4881..5426
                     /locus_tag="PK502_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPH00847.1"
                     /translation="MTEEIIIAGFGGQGVLSMGKILAYSGIMQDQEVSWMPSYGPEMR
                     GGTANVTVILSDSRISSPIIQEFDTAIILNQQSMDKFEPMVKPGGLLIYDPNGITRHP
                     VRKDIAIYQVEAAEEAARTKNPRTFNMIVLGAFLKVKPIVKLDNVIKGLEKSLPERHH
                     NLIPVNKEAILKGMELAKKYS"
     gene            complement(5589..6197)
                     /gene="nadD"
                     /locus_tag="PK502_08260"
     CDS             complement(5589..6197)
                     /gene="nadD"
                     /locus_tag="PK502_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HPH00848.1"
                     /translation="MKIGLFFGSYNPIHIGHLVIADFILEFSDLDEIWFVVSPQNPFK
                     SIDELAADEHRLNMTRLAIPEKNTRMKVCDVEMGMPRPSYTIDTLRRLEQESPGCTFV
                     ILLGSDNIAGIHDWKESAALINGWDFYVYPREGSFNPKNLPSGRFTLIDAPVIGISST
                     QIRHLIAHGVDASGYVPDDVWDYIIKNKIYGYGTTLTANADK"
     gene            complement(6194..6769)
                     /gene="gmk"
                     /locus_tag="PK502_08265"
     CDS             complement(6194..6769)
                     /gene="gmk"
                     /locus_tag="PK502_08265"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPH00849.1"
                     /translation="MDGKLLIFSAPSGSGKTTIVKHLLDCFPELEFSVSACSRPPRKG
                     EINGVDYHFLTVEEFKKRIAGNEFVEWEEVYPNLFYGTLWSEIHRIWNKGNHVVFDVD
                     VKGGLNLKKKFPDCSLAVFVMPPSMEDLKQRLIKRGSEDDESLALRLNKAKEEIGYAS
                     QFDVVLINNQVEKAFMEAERIVRDFIKIDQT"
     gene            complement(6821..7702)
                     /locus_tag="PK502_08270"
     CDS             complement(6821..7702)
                     /locus_tag="PK502_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPH00850.1"
                     /translation="MLVSMTGFGRAEMQLGSKKLIAEIRSLNSKQLDLSIRIPSIYRG
                     IEMDIRSIIAQQLVRGKVDFQLSFEETAESATTPINRDIFEGYLLQIKEIARQFEIDI
                     TNESLVQTILRLPDVLRVNGKDVVGEDELNSVKKLCSNAVESLVNFRRQEGSVLYNDI
                     KNRVLLILDLLQKVEPYEKQRIEDVKKRLREALKTLEDDVKINSDRFEQEVIYYLEKL
                     DITEEKVRLKNHCKYFLETAGLDEQVGRKLGFIAQEMGREINTMGSKANHSDIQKVVV
                     QMKDELEKIKEQSLNIL"
     gene            7932..8288
                     /locus_tag="PK502_08275"
     CDS             7932..8288
                     /locus_tag="PK502_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HPH00851.1"
                     /translation="MAILLLVISNTFMTMAWYGHLKFKELKWFQNFGIVGIVLISWGI
                     ALFEYFFQVPANRLGYKENGGPFSLLELKVIQEVITLLVFIIFTVLFFKTESIRINHL
                     IGFCFMVLAVYFVFKK"
BASE COUNT         2580 a         1995 c         1836 g         2315 t
ORIGIN      
        1 cctaaccgac caaaacggat tggatagcct ccaaattgcc cctggatact acaacttttc
       61 cgtcagggct tccggatata acatgagcag ccccggctac ttttttatcg aacccagcga
      121 taccctgatt gaagttttca tgcaacccgc aaccacccac agcctaaccc tgcaggttat
      181 tgatgaaaag ggaacctctc tacccggtgc ccaggttaca acactgaacc acggggctaa
      241 actaactaac gataatggtg aagcattttt ttcaaacgtt cctgaagggg aaatatcctg
      301 catggtttat aaggaaaact acatcaacaa tgttgcaggc gttaacttaa cctccgacag
      361 cactgttacc gttttacttt atgctaaccg ctacaccgct actttcttcg taaccagcaa
      421 gaacgtgcct gtttacaatg cccaggtgat tgttgagggg attgatactg cttacaccag
      481 cgctacaggg tatgccaata tcaaccttat accatttggt agcaacattc gctactcagt
      541 taagctcaac ggctacaaca cctaccttgg aacgtttaac ctggaggata gaaatattgt
      601 tgtgaatgta ccgctaaccc tcaccaccat agaacccacc aaaactaact cgtttgcaat
      661 ctaccccaac ccttcaaacg ggaacttcag cataaacctt gaaggtagct ttagcgttgc
      721 catttacaac ttggcaggat acaaggttta cctcagcaac cacgccgaga actccctaca
      781 ggttgacata cacagcctgc ctgcaggtat atacattgtt agggtaaccg gtaaaaactt
      841 ttcaacggtt gcaaagatta tcaagaagta aagcaaatgg ctacgaaaag ctaaaagggc
      901 agcaatgccc ttttttagtt ttaggtataa aatgtttcga ccaccccaca tgggttgtgc
      961 caggcataag aaagaatagc tgtatattta cattttataa cctttcctat ccaaatttga
     1021 caaaaagagt taaaaaacat caccaattgg caataaattg ttacagaaaa gtgaactttc
     1081 ttttaagagt taataatttt gattaacatt gtggcataaa aaggaaccgt aaaaagcgct
     1141 cattctccag gtatattttg gtatggccat atcttttact tacgtaaaac tagtgaagat
     1201 gcaataccag cgcacggagt aaacgtatga tggttttctg catctaagaa agagccatgt
     1261 tggagccaaa tgagatgtaa tagccagtaa acatggaagc tgcaatgcga tataagccaa
     1321 tacgttttta aacaccgaaa aaacaatacc atcatgaagg aaacgccaat accccgcgaa
     1381 attgttaagc agaagattgc tgaatcgaat cttccccggg tggggagagg ttccattcgt
     1441 gaaattgtgc atctggtaaa cgagattgaa aaggcctcag gacaaaagtt catccggatg
     1501 gagattggcg atcccggact tcagcctgct gaaattggaa ttgaagccga gattagcgct
     1561 cttcgaagtg gggtggcctc aaagtacccc ttggttgatg ggataccacc tctaaagaag
     1621 gagatatccc gctttgtgaa aaacttcatc aacattgatg ttgatgaaag aggttgcatt
     1681 cccaccgttg gctccatgca gggaggaatg gcggcatttc tgatgactac ccgctgcaat
     1741 acccgaaaaa ataccgtgct ctttatcgac ccggggtttc cagtccagaa gcagcagctt
     1801 aaggtgcttg gtattgacta tgaaaccttt gatgtgtata acttcagggg cgataggctg
     1861 cacgacaagc tggaatcgta cctgaaaaag ggaaatattt ccaccatact ctactccaat
     1921 cccaacaacc cctcatggat atgcttcacg gaaaaagagc taagaataat tggagaactc
     1981 gctactgcct atgatgctat agttgtagaa gacctggcct acctcggcat ggactttaga
     2041 aaagacctgt ccaaacctgg tcaaccaccc taccagccca gtgtggccaa ctataccaac
     2101 aactggctaa tgctgatctc aagctcaaag attttttcgt atgccggaca acgaattgga
     2161 tgcatagtgg taagcaatga actttacaat cgccatttcc ccgaccttaa acggttcttc
     2221 tcgtccgatg agtttggcta cgccatgata tatggtgctg tttaccccct atcgtcgggt
     2281 gtttgccact cggcacagta tggatttgca gccatgctga aagcagcaaa tgacggagtt
     2341 tacaattttg tcaacgatgt aagggagtac ggcgaaaaag ccaaagtcat gaagaagctt
     2401 ttcaccgaca acgggtttaa aatagtttac gacagggatg aaggcgaacc gcttgccgat
     2461 ggcttctact ttaccatatc ctaccccggg ctaaccggcg aagagctaat tgaggaaatg
     2521 ctctactatg gcattagtgc catttcgctc ctaatcaccg gaagcgaacg ttcggaaggc
     2581 ttgcgtgcat gtgtttcaca agtgcagaga agccagtttt cggacttaga gtaccgcctt
     2641 aaaaaatttc acgaagatca tccaaccaag taatattata gcaagctaac tattatacaa
     2701 acacttttaa atttttcaat gtcatggcaa aaataagagg agcaattgtt gttgatgtgg
     2761 aaaggtgcaa gggatgcagc ctttgtgttg ttgcctgccc ttcgagcgtc attgcgctgg
     2821 caaaggaggt aaacggcaag ggttacaact atgcctacat ggtcaacccc gatgcatgca
     2881 caggatgtgt taactgtgcc attgtatgtc ccgacactgt tatttcagtt tacaaagtta
     2941 aggctgatgc agtagcctag agtagcatta cttaaagttg aaaatacaaa tttcaaaaga
     3001 aatggcagaa ctaaaactaa tgaaggggaa cgaagctata gccgaggcag ctatccgatg
     3061 tggttgcgac gggtattttg gctaccccat cacccctcaa tcagaaatca tggaaaccct
     3121 tatggccaga cagccgtggg atgaaacagg aatggtggtg cttcaggcag agagcgaggt
     3181 ggcatccata aacatggtat atggaggtgc cgcttgtgga aaaagagcca tgacctcgtc
     3241 gtcaagcccc ggtataagcc taatgcagga aggtataacc tacattgccg gatctgagct
     3301 tccctgtcta atagttaatg tagttcgtgg agggcctggc cttggtacca ttcaacctgc
     3361 ccaaagcgac tactttcagg ctgttaaggg aggcggacat ggcgactaca agcttattgt
     3421 tctggctccg gcctcagtcc aggaaatgta cgattttgta gatttagcat ttgagctggc
     3481 ctttaaatac cgtaatccgg ccatgattct ctccgatggc gttattggcc aaatgatgga
     3541 gaaagttcaa ataggcgatt acaagccgcg gcttactgac gaggaagttg taaaacgtta
     3601 tggttcgtgg gcaaccaccg gtaagccttc aacccgtgag cgcaacatta tcacctccct
     3661 tgaccttgat gccaacagcc aggagctgtt caaccttaag ctgcttaaga aatatgcgga
     3721 aatagaaaaa aatgaaatcc gtttcgagga aatttcctgc accgacgcag aatacttgct
     3781 ggttgcttac ggctcatcga gcagaatatg ccaaaaaacc gttgatttgg cccgtgaaaa
     3841 gggaatcaag gtaggattgt tacgacccat taccctttac ccttacccct atggagccat
     3901 tcaaaagctt gtgccacaac taaagggaat tttagctgtc gaaatgagcg ccggtcagat
     3961 ggtggaggat gttcgactag cggttaacgg gaaagtccct gttctccact acgggagaat
     4021 gggtggtgta atacccaccc caaatgaggt gttaaaggtt ctggaagata aacttattaa
     4081 aggctaaaac gatggacgtt aaggacataa tcaaagagga aaatttggtt tacagcaaat
     4141 caaaagttct caccgataca attatgcact actgcccggg ctgcacccat ggtgttgtgc
     4201 acaaggtaat cgccgaggta attgaagagc tgggcattca ggataaaacc ataggggttt
     4261 caccagtggg ctgtgccgaa tttgcctata actacctcga tatcgactgg caggaagctg
     4321 ctcatggtag agctcctgct ctggctaccg ccattaaccg actctacccc gataagtatg
     4381 tgtttaccta ccagggtgat ggcgatttag cttccatcgg gactgcagag ataatccatg
     4441 catgcaaccg tggtgaaaac atagtagtaa tattcatcaa caacggaata tacggtatga
     4501 ccggtggtca aatggcgcct actacgctga tagacatgcc gacctccacc acgccctacg
     4561 ggcgtaaagt tgaccttaat ggttacccgt taaaaattac cgaccttgtg gcacagctgc
     4621 ccggtacatg ctttgttacc agacattcgg cccacactcc agctgccgtt agaaagctta
     4681 agaaagccgt aaccaaagct tttcagaata cccgaaggaa aaaaggagta tcgttcatcg
     4741 aagttgtttc aacctgcaac tcgggatgga agcttagccc tgttgaatcg aacaactgga
     4801 tggttgaaca catgttcccc tactatccgc tgggcgatat gaaagatgag taaccggaaa
     4861 cctattaaaa cactacgaac atgacagaag aaatcatcat agccggattt ggaggtcagg
     4921 gggttctttc aatgggcaaa attttggcct actccggaat aatgcaggat caggaagtat
     4981 cgtggatgcc atcctacggc cccgaaatgc gaggaggaac tgctaatgta accgttatcc
     5041 ttagcgattc acgaattagc tcacccatta ttcaggaatt tgacacggcc attatcctca
     5101 accaacagtc gatggacaag tttgaaccaa tggttaaacc aggcggattg ctgatttacg
     5161 atcccaacgg gattacccgc catccggttc gtaaggatat tgccatttac caggttgaag
     5221 ccgccgagga agccgccagg acaaaaaatc caagaacctt caacatgata gtgctagggg
     5281 ctttcctaaa ggtaaaacct attgttaaac ttgataatgt gataaaaggg ttggaaaaat
     5341 cgcttcccga acgccatcac aacctaattc cggtgaataa ggaggctatc ctgaaaggaa
     5401 tggaactagc aaaaaaatac agctagctaa agttgaatgt taatggtatt ctccactaaa
     5461 agccccagca tttggggctt ttttctaact caaataacgc atatctcaat ttcgaactag
     5521 catttacttc acctaccgaa cgcagctatc aatcctaatg ctcataaggt gaattagcaa
     5581 accaaaattt atttgtcagc attagcagtg agcgttgtgc cataaccgta aatcttgttt
     5641 tttatgatgt aatcccacac atcatcggga acgtatccgg aagcatcgac accatgggca
     5701 atcaggtggc gaatttgagt tgacgaaatc cctattacag gagcgtcaat aagggtaaag
     5761 cgcccggaag gtaaattttt aggattaaag gaaccttcac ggggatagac ataaaaatcc
     5821 cagccattta tcagggcggc ggattccttc caatcatgga taccagcaat gttatccgaa
     5881 cctaagagaa tcacaaaagt gcacccgggg ctttcctgct ccagtctcct gagcgtgtca
     5941 attgtgtagg agggtcgggg cattcccatt tcaacatcgc acaccttcat ccgggtgttc
     6001 ttttcgggaa tagccagcct tgtcatgtta aggcgatgct catcggcagc aagctcatca
     6061 atgcttttaa aaggattttg agggcttaca acaaaccaaa tctcatcaag atcggaaaat
     6121 tcaagaataa aatcggctat aaccagatgg ccgatgtgga tggggttgta ggaaccaaaa
     6181 aataagccta ttttcatgtc tggtcaattt ttatgaaatc cctgacaatt ctttcagcct
     6241 ccataaaagc cttctctacc tgattgttaa ttaaaacaac atcaaactgg ctggcatacc
     6301 caatttcctc ctttgctttg tttagcctta gtgctagcga ttcatcgtct tcggagcctc
     6361 gttttataag cctctgctta agatcctcca tagagggggg catgacaaac acagccaggg
     6421 agcaatcggg aaatttcttt ttcaggttca atccgccctt aacatccaca tcaaacacca
     6481 catgatttcc cttgttccat attctatgaa tttcagacca taaggttccg tagaacaagt
     6541 tcggatacac ctcctcccat tcaacaaact cgttgccagc aatacgcttc ttaaattcct
     6601 ccaccgtgag gaaatggtaa tctactccgt taatctcacc tttcctggga ggccggctgc
     6661 atgccgaaac cgaaaattct aactcgggaa agcaatcgag caggtgctta acaatggttg
     6721 ttttgcccga tcccgaaggt gctgagaata taagtagctt gccatccatg ccgttaagtg
     6781 aggagtaata gtagcaaagt ttagaagaat ttacaaacgg ttaaagaata ttgagcgatt
     6841 gctccttgat tttctccagc tcatccttca tttgcaccac aactttctga atatctgaat
     6901 ggttggcctt agaacccatg gtgttgattt ccctccccat ttcctgcgca ataaagccca
     6961 gtttccttcc aacctgctca tccaaccccg ctgtttccag gaaatacttg caatggtttt
     7021 tcaaccttac cttttcctcc gtaatatcga gcttttcaag gtaatagata acctcctgct
     7081 caaaccgatc ggaattaatc tttacatcat cttcgagcgt ttttaacgcc tcccgaagcc
     7141 ttttcttcac atcctcgatt cgctgctttt cgtagggttc aaccttttga agcaaatcaa
     7201 gaatgagcaa aaccctgttt ttaatatcgt tatacagcac gctgccctcc tgcctgcgga
     7261 agtttacaag actctctacc gcattggagc aaagtttttt aactgaattg agctcatcct
     7321 cacccacaac atccttgccg ttaacacgaa gcacatcggg taaccttaga attgtctgca
     7381 ctagcgattc gtttgtaatg tcaatttcga actgacgggc tatttcttta atctgcagca
     7441 ggtagccttc aaaaatatcc ctgttaattg gggtagttgc cgattcggct gtttcctcaa
     7501 aactcagctg aaagtcaacc tttcccctga ccagctgctg cgcaataatg gaacggatat
     7561 ccatctcaat gcccctgtag atggatggaa tcctgatgct taaatcgagc tgcttgctgt
     7621 tgagcgagcg aatttccgct ataagttttt tactgcccag ctgcatttcc gcccttccaa
     7681 aacccgtcat cgaaactagc ataattcctg ttttaatttg tacaaaggta atttagaatt
     7741 attcattata gccaaccagc gcacgaaatc atcattcaag ctgaaattca tgtttttaac
     7801 ggacggtgct cctctccaaa cacttttacc tggtttcgat gtaaactaca gtaaggcata
     7861 attaccggtg gaatgctatc tttgcacccg caaacaatgt aaatgtaagg tatatggcaa
     7921 acagagggtt gatggcaatt ttactgcttg ttatctcgaa cacctttatg accatggcat
     7981 ggtatgggca tttgaaattc aaggagctca aatggtttca aaattttggt atagttggca
     8041 tagttctgat cagctggggt atagccctct tcgagtattt ttttcaggtt cctgccaacc
     8101 gtttaggtta caaggaaaat ggcggcccat tttcactcct ggagctaaag gttattcagg
     8161 aagtgattac cctgctggta tttataattt tcaccgtgct attcttcaaa accgaaagca
     8221 taagaataaa ccacctaatc ggcttttgct ttatggtgct agcagtttac ttcgtattca
     8281 aaaagtgatg ggttgtgctg gtactaaaac gaagaaggcc tttcggcctt cttctcccca
     8341 aacacacgat ttactgaaaa aaccaactgg tttccatcaa actacctaga aaaaatatac
     8401 aaactaacct cccagtaaac tatggaaacc aaactgaata ggacaaatat aataccaaat
     8461 ttttattgaa tgaaattata acgaaaatta ttactacgaa aaaattgaaa aatttttaac
     8521 gactgttgtt tattatgaac cacaccctgt ataattaaaa ttttcaactt tccctttgcc
     8581 atttatctac ttataccggt acttcgtttt acgttttagg acattaatca aagaaaaggc
     8641 cttacagttt gcccaagctg taattatgta atatctttac gctgataagc aaaaataaat
     8701 atggattccc tatatattga agcaga
//
LOCUS       DAOPHX010000107         8681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_10187_length_8681_cov_5.606191, whole genome shotgun sequence.
ACCESSION   DAOPHX010000107 DAOPHX010000000
VERSION     DAOPHX010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10187_length_8681_cov_5.606191"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PK502_08280"
     CDS             complement(<1..203)
                     /locus_tag="PK502_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00852.1"
                     /translation="MFKNRKFLGAAIALLAVATCYVFIAFIPSAHSKPPIEKPDTVLQ
                     DGYIFPIPIPQNLSFAGENVPLD"
     gene            complement(400..1431)
                     /locus_tag="PK502_08285"
     CDS             complement(400..1431)
                     /locus_tag="PK502_08285"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPH00853.1"
                     /translation="MTTHHIHIATLKRFGQLLLLLLLIISCQARQTATYTKVNGFTQG
                     TTFNIIYSDSAGRDFSKAFDSIFHAVDRSMSVYNDSSIISRLNRNEIDTVDALLAEVI
                     AISDSIYHETDGAFDITVGPVVRALGFGPEQHAGIDSAQIRALQRLIGMGKINMAGNR
                     LQKDDPKIQIDVNAVAQGYTSDLVARFLSSKGISNFMVEVGGEIMAMGISSRGTPWIV
                     GIDKPVENALPGENLQAKFYFSGSRGMSTSGNYRKFIEVNGIKYAHTINPKTGFPAMS
                     NLLSATVIAPTAARADALATAFMVNGLEWSRNFVNSHTGVEAYLIYSDSTGKFNEWVS
                     EGLKSSIAQ"
     gene            complement(1561..2724)
                     /locus_tag="PK502_08290"
     CDS             complement(1561..2724)
                     /locus_tag="PK502_08290"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPH00854.1"
                     /translation="MNNFEFFNPVRILFGNDKIEKIGMQLNPTDRILMTYGGGSIKKN
                     GTYERIKAALSNFSVFEFGGIEANPKYETLMKAVEVVKKNNVDFILAIGGGSVIDGTK
                     FIAAASLYEGSDPWDLLIKNIVVTKAIPFGVVLTLPATGSEMNSGAVISRLETREKYS
                     FGSPVLFPKFSVLDPTLTFSLPPHQVANGIADAFIHVMEQYMTFPSDSPIQDRFSESI
                     LQTLIEVGPQTLKNPSDYSLRANFMWSCTMALNGLLSTGVTTDWATHMIGHEFTAFFG
                     IDHGATLAAVYPALLTELQEPKHEKIIQYGSRVWGLSGADLTARNVINKTEDFFRSLG
                     IKTRLCEYGLKQEDVAPIVERFRTRGWKLGERKNINHEVVEAILKRALEPYRS"
     gene            3036..3560
                     /locus_tag="PK502_08295"
     CDS             3036..3560
                     /locus_tag="PK502_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00855.1"
                     /translation="MNKLTLGKYHILVVVAVLAATVVLLSCRQSKQENVKSSADKARE
                     NVVSWMESMKLEYPGYQPAEFGELTPRYNKSNITFHLYNLIEQERSKPSPSKRAIDSL
                     ENLLRDNPGELLGYSITHKYNVKDISGEIRQVESLFLLDTLLRVITVLDADAYDQIMD
                     EELFFRPESKDSTL"
     gene            complement(3727..4617)
                     /locus_tag="PK502_08300"
     CDS             complement(3727..4617)
                     /locus_tag="PK502_08300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPH00856.1"
                     /translation="MRKIAYLLTAGVVAVSATTLRAQQEPIFTHYFQNPVSINPAIAG
                     TVRNLNLSLLSRLQWVGLEGAPKTFSLSAHMPWPEKRMGIGLNIMNDDTWPVSNTHIS
                     ASYAYRLRLTDEITLSLGIKGGFTYYHASLTNLQINDPNDPEFTQNESRFYPNLGAGA
                     YIYSGEFYAGLSLPRFLQTSFNKKFDKTLKSPLYLMGGYNLEISPSWVFMPSMLMGAM
                     IGLPMSCDLTAQFLYQSKFYFGTHYRIGDALGIFFDMKVNEYLSFGYAFDFSLNKLSR
                     INTGTHELMLSYAFAPKWKF"
     gene            complement(4647..>8681)
                     /locus_tag="PK502_08305"
     CDS             complement(4647..>8681)
                     /locus_tag="PK502_08305"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPH00857.1"
                     /translation="VETFRYYVEDADGDYSLATVTVTVIEKPNYVPIANDDYRGCSFN
                     SSVTVDVLSNDTGIDDVPLTLSININPTDGNAQVNPDNTITFTPFDGFIGLTTFGYRV
                     TDANGDSDEAMVFINVKAGINAVPLARDDNAHTFINKSVEINVLANDEGLDDGFGALT
                     IRQQPAFGTVVVNANRTLTYFPGYMFVGSDSFSYMVEDVDGDYSVAIVTVVVDEKPDS
                     KPVANDDSRGCSFNSAVTVDVLINDSGLDDVPLVVSVTEAPQSGSAVPNPDNTITFTP
                     EANFTGTITFRYTVTDVDGDFDDAQVTIHVKAGQNIVPYAVPDNATTLVNKPAVVNVL
                     ANDTGLDDGFGSLTVFEQPKFGSVVVNSNRTVTYTPSYMFIGVETFRYMVTDVDGDYA
                     IGTVTITVIEKPNSIPVANDDLRGCSFETPVVVDVLVNDTGLEDVPLTLTISELPTIG
                     STVVNPNNTITFTPPAAFTGIATFRYTVTDSDGDSDDALVTIRVKEGENYVPHSNDDA
                     ASTIINTNVDINVLANDTGLDDGFERLIIFEMPSFGTVTVNENRTVTYTPSYMFLGTD
                     TFRYWVEDVDGDYSIATVTVEVTERPNYLPVANDDYRATSLNTPRIVDVLVNDTGLDD
                     TPVEVSITNQASHGTTVVEADNTVTYTPQNSYLGADHFSYRVTDRDGDWDEATVFIDV
                     KPVNMIPVAVDDYVQTYVNQPVDIHVLENDSLLYEGIKSLQVFSAPKWGTVQVNDNHT
                     ITYTPSYFFIGNDEFKYLVEDVDGDYDIATVYVVVLATQNAIPVANDDHRATSKNTPL
                     DINVLINDDGLDDGWIRVFIDEFPDAATGTAAVKPDNMVSFIPATDYLGLSTLKYFIV
                     DRNNDTSNVATVTVNVKEVNFIPVANADTASTNMNVPLLVDVVNNDTGLDDGLDYIKV
                     MGKPLHGFAYAFDNHNIKYFPSSWFVGTDSLTYLVADEDGDYDIGKLYITVNERPDHK
                     PLANPDGRGTTTNTPVTVDVLFNDTGLEDGGIKVLLNIFPINGNVLLHADNTVTYTPN
                     TGFLGEDVFYYQVCDFDSDCSSAAVTINVKNANLVPIAYNDTVTTFKNKAISINVLFN
                     DRNLNDGGIRTVVFSNPTNGAVSVNPDNSILYTPANNFFGVDSLWYYVADVDGDYSLA
                     KVVIDVLNRENFTPQANDDDVEIFMDTETTIDVLTNDTGLNDGVKALAVETSPTNGIC
                     RITSDFKVYYSPLTGFVGTETFTYRVTDRDDESSVAYITVRVVPDPDGKVVVPEAFSP
                     NGDGFNDTFEVLNIGQFQHITLKVYNRWGNLVYKSDRYKNDWDGTANVSLSLGSKLPA
                     GTYYYLLEILDTGKTYKGSVFIKR"
BASE COUNT         2246 a         1908 c         2140 g         2387 t
ORIGIN      
        1 taatcgagtg gaacattttc gccggcaaag gagaggtttt gggggattgg gatgggaaaa
       61 atgtagccat cctgaagcac cgtatcgggc ttttcgatgg gcggtttact gtgtgcagat
      121 ggtataaagg caatgaatac atagcaggta gctactgcta acagggctat agctgctcct
      181 aaaaattttc gatttttaaa cattttctaa ggagtgtttt gggcatgcag ctttatgcaa
      241 ccgggcgcaa aattaccttt tttaaacaaa ttgttgacat ctataaacca tgtaacctat
      301 gccggatcat actgcattca tggcaaaatt ccaatgcatt cggcagtaca aatggtagtt
      361 ggttgggata aaaaatcttt gtagaggtaa gcctaacggc tattgggcaa tgctgctttt
      421 aagcccttcg ctaacccatt cgttaaactt tccggtggag tcggaataga taagataggc
      481 ctcaactccg gtgtgggagt tgacaaaatt ccggctccac tccaacccgt ttaccatgaa
      541 ggctgttgcc agggcatcgg cccgagcagc agttggcgcg attactgttg cgctcagcag
      601 gttgctcatg gccggaaatc ctgttttggg gtttattgtg tgggcgtact ttatgccgtt
      661 tacctcaata aacttacgat agttgcccga tgttgacata ccccgtgaac ctgaaaagta
      721 gaatttggcc tgaaggtttt caccgggtaa agcgttttct acaggcttgt caatgccaac
      781 tatccagggt gtgcctcggc tgctgatccc catagccata atttctccac ccacctctac
      841 catgaagttg ctaattcctt ttgaacttag gaacctggct accaggtcgg aagtgtagcc
      901 ctgggccacc gcatttacat caatctgaat ttttgggtcg tctttctgca gtcggttgcc
      961 agccatgttg attttaccca taccaatcag ccgctgaagt gcccttatct gtgcgctgtc
     1021 aatgccggca tgttgttccg ggccaaagcc caatgcccta accactgggc caacagtaat
     1081 atcgaaggcc ccatcggttt cgtggtaaat agaatcagag atagcaatta cctcggccag
     1141 cagggcatca accgtatcaa tctcgtttct gtttagacgg gagattatgg agctgtcgtt
     1201 gtaaaccgac atggagcggt caaccgcatg aaaaatcgag tcaaaagctt ttgagaaatc
     1261 cctgcctgcc gaatcggagt aaatgatatt gaaggttgtt ccctgcgtaa aaccatttac
     1321 cttggtgtag gttgctgttt gccgtgcctg gcaggaaatt atcagcagca acagcagcaa
     1381 taactggcca aatcgcttta atgtggctat gtgaatatgg tgtgtagtca tagtttgcgt
     1441 aagaagtatt taaattgcac aaacagcatg attaggtacg ggataatggc tgataggcca
     1501 tgctattagg cttaaccctt tcttcaacca atcaatctaa attataaaat ctagcagagg
     1561 ttaagaccta tagggttcaa gcgcacgttt gagtattgcc tccacaactt catggttgat
     1621 gttctttctt tcacccagct tccatccccg cgtgcggaac ctctcaacaa ttggagcaac
     1681 atcttcctgc tttaatccgt actcgcacaa gcgggtctta atccccagcg aacggaaaaa
     1741 atcttcggtt ttgtttatta cgtttcgtgc ggttaaatct gctccgctca gcccccatac
     1801 cctgctgccg tactgtataa ttttttcatg tttgggttcc tgcagctcgg taagtagggc
     1861 cgggtaaact gcggcaaggg tggcaccatg gtcaattcca aaaaatgcag tgaactcatg
     1921 cccaatcatg tgggttgccc agtcagttgt tactccagtt gatagcaagc cgtttagggc
     1981 catggtgcag ctccacatga agttagcccg aagggagtaa tcggatggat ttttcagggt
     2041 ttgcgggcca acttctatga gggtttgcag aattgactcc gaaaagcgat cctgaatagg
     2101 cgagtcggag gggaaagtca tgtactgctc cataacgtgg ataaaggcgt cggcaatacc
     2161 attagctacc tgatggggag ggagcgaaaa ggtgagggta gggtcgagaa cggagaattt
     2221 tgggaacagc accgggcttc caaagctgta cttctcgcgg gtttcaagac ggcttatcac
     2281 cgcacccgag ttcatttcgg aacctgtagc cggaagcgtt aaaacaaccc caaatggtat
     2341 ggccttggtt actactatat tctttattag cagatcccat ggatcggacc cttcgtatag
     2401 gctggctgct gcaataaatt ttgttccgtc tattactgaa cctccgccaa tcgccagtat
     2461 gaagtcaaca ttattttttt taaccacctc taccgccttc atcagggttt cgtacttggg
     2521 gttggcttca attcctccga attcaaaaac gctgaagttg gaaagtgccg ccttaatcct
     2581 ttcgtaggta ccgtttttct taatgctgcc accgccatag gtcataagaa ttctatcggt
     2641 tgggttaagc tgcattccga ttttttcgat tttgtcgttt ccgaaaagaa ttctgaccgg
     2701 gttaaaaaat tcaaaattat tcatatcgtg ttgtttttat ggtattctgt caagggggcc
     2761 atgttggaaa tctactagca atctaaatgg ctatttgaaa ataacagcat ggctgcttgc
     2821 tacaaaatta gtaaatctct gcggaaagga ttcaagccgg tatggcataa atttttaggg
     2881 attatccttg ttggacaata aagataattc cagctacgag cacgcccctt ccaaaccttc
     2941 tcgctagtca atactttaaa agatttaaaa aagagtccgg aatattctaa gtataaattt
     3001 agtagcttta tcaggcaaaa aataaggggg tggctatgaa taaacttact ttaggaaaat
     3061 atcacatcct tgtagtcgtg gctgttctgg cagccaccgt tgtgctcctt tcatgcaggc
     3121 agagcaagca ggaaaatgta aaaagttctg ctgataaggc cagagaaaac gtggtgagct
     3181 ggatggagtc aatgaaatta gagtatcctg gttaccagcc agccgaattt ggcgagctaa
     3241 ctcccaggta caacaaaagc aacattactt tccacctcta taacctcata gagcaggaaa
     3301 ggagcaagcc aagtccaagc aaaagggcta tagacagcct ggagaacctt ttaagggata
     3361 atcccggtga gctgcttggc tattcgatta cccataagta caacgtaaag gatatctcgg
     3421 gtgaaattcg gcaagttgaa agcttatttc ttctcgatac gttactccgt gtaattacgg
     3481 tgcttgatgc ggatgcctac gaccagataa tggatgagga gctatttttc agacccgaaa
     3541 gtaaagactc aactctataa gcatttaggc tctttttaaa ggcagccttt aaaacctgca
     3601 tattcacctt tacgatgaac atgcaggtaa ttttgcagtg ctgcttaccc tgcaaaataa
     3661 tcatggttgc tttggactaa acttatagag ttttgcatgg taatgcccaa attcatatct
     3721 cgataatcag aatttccact tgggagcaaa ggcgtatgaa agcattaact catgtgttcc
     3781 ggtgttgatt ctggagagtt tattaagcga gaaatcgaag gcatagccga atgataggta
     3841 ttcgttaacc ttcatatcga agaaaattcc cagcgcatcg ccaatacggt agtgggtgcc
     3901 aaagtaaaat ttgctctggt agagaaactg ggcggtcagg tcgcacgaca tgggtagtcc
     3961 aatcattgca cccattagca ttgatggcat gaaaacccag ctggggctga tttccaggtt
     4021 gtagcccccc atgaggtaga gtggactttt aagggttttg tcgaactttt tattaaatga
     4081 agtctgaagg aaacgtggta gggaaaggcc tgcgtagaat tcgccggagt aaatgtaagc
     4141 acctgcaccc aggttagggt agaatcggct ttcattctgg gtaaactccg ggtcgtttgg
     4201 gtcgtttatc tgaaggttgg taagcgaggc atggtagtag gtgaaacctc ctttaattcc
     4261 aagcgaaaga gttatttcgt cggttagtct aaggcggtat gcatacgatg ccgagatgtg
     4321 tgtattgctg accggccatg tgtcatcatt cattatgtta aggcctattc ccatcctctt
     4381 ttcgggccag ggcatgtggg ccgaaaggct gaaggttttg ggagctcctt ccaggccaac
     4441 ccattgtaac ctggaaagga ggcttaggtt aaggtttcgt accgtaccgg caatggcggg
     4501 gttaatggac accggattct gaaagtagtg ggtgaaaatt ggctcctgct gtgccctcag
     4561 ggtggttgcc gaaacggcaa ccactcctgc tgttagtaaa taggctattt ttctcatttt
     4621 tcaaaaaaat atcgacttta acttgattat cgcttaataa acacgcttcc cttgtaggtt
     4681 ttacctgtat ctaaaatttc gagcaggtag tagtaggttc cggcaggaag tttagagcct
     4741 agcgaaaggg atacgttagc tgttccgtcc cagtcgtttt tatacctatc gcttttgtaa
     4801 accaggttac cccaacggtt gtaaaccttc agggtaatgt gttggaattg ccctatattt
     4861 agaacttcga aggtgtcgtt gaatccatca ccgttgggtg agaaggcctc gggtaccaca
     4921 accttcccgt cggggtcggg tacaacccga acagttatgt atgccacgct gctctcatcg
     4981 tccctatcgg ttacccggta ggtaaatgtc tcagtaccaa caaaaccggt aagtggggag
     5041 tagtaaacct tgaaatctga ggttatacgg catatcccat tggttggtga agtttcaact
     5101 gccaacgctt ttactccatc gtttaacccg gtatcgttgg ttaacacgtc aatagtggtt
     5161 tcggtatcca tgaaaatttc aacatcatca tcattggcct gaggtgtaaa gttctcacgg
     5221 ttgagaacat ctataactac cttagccagg ctgtagtcgc catccacatc agcaacatag
     5281 taccaaagcg agtcaacacc aaagaagttg ttcgcggggg tgtagagtat ggagttgtcg
     5341 gggttaacag ataccgctcc gttagtaggg ttgctgaaca caaccgttct aattccccca
     5401 tcgttcaggt tcctgtcgtt aaacagtaca ttaatggaga tggccttgtt cttaaaagta
     5461 gtaacggtat cgttgtaggc aataggtaca aggttggcgt ttttaacgtt gatggtaacc
     5521 gcagcgcttg agcagtcgct gtcgaagtcg caaacctggt agtagaatac gtcctctccc
     5581 aaaaagccag tgttgggtgt gtaggttacc gtgttatctg cgtggagcag cacatttccg
     5641 ttaatgggga atatgttgag cagcaccttt ataccaccgt cctccaggcc ggtatcgttg
     5701 aaaagaacat ctacggtaac gggggtgttg gtagttgtac cccgcccatc agggttggct
     5761 aagggtttgt ggtcgggcct ttcgtttacg gtgatgtata gctttcctat gtcgtaatcg
     5821 ccatcctcat cagctaccag gtaggttaac gagtcggttc caacaaacca gctggatggg
     5881 aaatacttga tgttatggtt gtcgaaggcg taggcaaagc catgaagcgg tttacccatt
     5941 accttgatgt agtcgagccc atcgtcgagt ccggtgtcgt tgttcaccac gtcaacaagc
     6001 aggggaacgt tcatgttggt tgaggcggtg tcggcattgg ccacagggat aaaattaacc
     6061 tccttgacgt taaccgttac ggtagccaca ttgctggtgt cgttgttcct atcaactata
     6121 aagtacttaa gtgttgacag acccaggtaa tcggtagccg gtataaatga taccatgtta
     6181 tcgggtttaa ccgcagcggt tcccgtagcc gcgtcgggaa actcatcaat gaataccctt
     6241 atccaaccgt catcaagccc atcatcgttg atgagcacgt tgatatcgag tggggtgttt
     6301 ttcgatgtag cccgatgatc atcattggca accggaatgg cgttttgcgt ggcaagaacc
     6361 accacgtaaa ccgtagcaat atcgtaatcg ccatctacat cctcaacaag gtacttaaac
     6421 tcatcatttc caataaagaa gtatgagggt gtgtaggtaa tcgtatggtt gtcgttaacc
     6481 tgaacagttc cccattttgg ggcagagaac acctggaggc tctttatccc ctcgtagagc
     6541 agggagtcat tctcaagaac atggatatca accggttggt taacgtaggt ttgcacgtaa
     6601 tcgtcaacag caacagggat catgtttaca ggtttaacgt cgataaatac cgtagcttca
     6661 tcccagtcgc catccctgtc ggttaccctg tagctgaaat ggtcggcacc caggtagctg
     6721 ttttgtggtg tataggtaac cgtattatct gcttcaacaa cagtagttcc gtgagaggct
     6781 tggttggtga ttgaaacctc tacaggagta tcgtccaggc cggtatcgtt taccaaaaca
     6841 tctactattc taggcgtgtt aagggatgtg gctctgtagt catcgttggc cacgggtaag
     6901 tagttggggc gttcggttac ctcaacggta actgttgcga tggagtagtc gccatccaca
     6961 tcctctaccc agtacctgaa tgtgtcggtt cccaagaaca tgtatgaggg agtgtaggtt
     7021 actgttcggt tttcgttcac agtaacagtg ccaaaagagg gcatttcaaa gatgatgagt
     7081 ctttcgaagc catcgtctag tcctgtgtcg ttagccagaa cgtttatgtc aacatttgta
     7141 ttgataatgg ttgaagccgc atcgtcgttg gaatggggta cataattctc accttccttg
     7201 acccgtatgg taaccagcgc atcgtcgcta tcgccatcgg aatcggtaac cgtgtagcgg
     7261 aaggttgcaa taccggtgaa agcagctgga ggggtaaagg ttatggtgtt gttaggatta
     7321 acaaccgtgc tgccgattgt gggcagctca gaaatagtaa gcgttagggg aacatcttcc
     7381 aaccctgtat cgttgactag aacatcaaca accacaggag tttcaaagct gcacccgcgg
     7441 aggtcgtcgt tggctaccgg tatggagttg ggtttttcaa tgaccgtaat ggttacggtg
     7501 ccaatagcgt aatcgccatc cacatcggtt accatgtagc ggaaggtttc aactccaata
     7561 aacatatagc tgggggtata ggttaccgtg cggttagagt ttacaaccac acttccgaac
     7621 ttaggctgct cgaaaactgt cagcgagcca aacccgtcgt ctaaacctgt atcgttggcc
     7681 agaacattca caacagcagg tttattcacg agggttgttg cattgtcggg aacagcatac
     7741 ggcacaatat tctgaccagc cttcacatgg atggtaacct gtgcatcgtc gaagtctcca
     7801 tcaacatcag taacagtata gcggaaggtt atagttccgg tgaagttagc ctcgggggta
     7861 aaagttatgg tgttgtcggg gtttggaacg gcgctgccgc tttggggtgc ttctgtaacg
     7921 gaaacgacaa gcggcacatc atccagtcca ctatcgttaa ttagcacatc aacggtaacg
     7981 gccgagttga agctgcagcc ccgcgagtcg tcgttggcaa cgggtttgct gtcgggcttt
     8041 tcatccacaa caacggtaac aatggccaca gagtagtcgc catccacatc ctcaaccatg
     8101 tagctgaagc tatcggagcc tacaaacatg tatcccggga agtaggttag ggttcggttg
     8161 gcattcacca caacagttcc aaaggcaggt tgttgcctga tggttagtgc gccaaaaccg
     8221 tcgtcaagcc cctcatcgtt agccagcaca ttgatttcta cggatttgtt gataaaagta
     8281 tgggcattat catcgcgtgc caggggtaca gcatttatac ctgccttcac attgatgaat
     8341 accatggctt catcgctatc gccattggcg tcggtaaccc tatacccaaa ggtggtcaat
     8401 ccgataaacc catcgaatgg agtaaatgta atggtgttat cggggttaac ctgtgcattg
     8461 ccatcagttg ggtttatgtt gatagatagg gtcaaaggca catcgtcaat tccggtatcg
     8521 tttgaaagca catcaacggt aacgctggag ttgaagctgc atcccctgta atcgtcattg
     8581 gctatcggaa cgtagtttgg cttttcgatg acggtaacgg taacggtagc cagcgagtaa
     8641 tcaccatcag catcctcaac atagtagcgg aatgtttcaa c
//
LOCUS       DAOPHX010000108         8646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_10258_length_8646_cov_6.139332, whole genome shotgun sequence.
ACCESSION   DAOPHX010000108 DAOPHX010000000
VERSION     DAOPHX010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 8646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8646
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10258_length_8646_cov_6.139332"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PK502_08310"
     CDS             complement(<1..506)
                     /locus_tag="PK502_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPH00858.1"
                     /translation="MQGVLYDFTGLIQSIDAVFRASMSDLWATVLEYAIIGIIYLVIY
                     ALIGLFLVYAERKVCAFIQVRLGPNRVGPKGFFQTIADFIKLLLKENIKIKNADGVLF
                     NLAPFIVIVASFLTFGTVPFAKGLQGIDLNIGVFFIMAVSSMGVVGILLGGWSSNSKY
                     SLIGAMRSG"
     gene            complement(553..2149)
                     /locus_tag="PK502_08315"
                     /pseudo
     CDS             complement(553..2149)
                     /locus_tag="PK502_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
     gene            complement(2156..2743)
                     /locus_tag="PK502_08320"
     CDS             complement(2156..2743)
                     /locus_tag="PK502_08320"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HPH00859.1"
                     /translation="MAIKSMKESDFKDTDTLDQQMLEENGIFFTTVDSILNWGRSNSI
                     WPLTFATSCCGIEMMATAAARHDFARFGIEVYRASPRQADVIVVAGTIVNKMAPVLRR
                     LYDQMAEPKYVVAMGACAVSGGPFFYNSYHVLKGVDHLIPVDVYIPGCPPRPEAFLYG
                     IMQLQRKIKKETIKNKYARKNVANNYDEKNPLPEK"
     gene            complement(2734..3084)
                     /locus_tag="PK502_08325"
     CDS             complement(2734..3084)
                     /locus_tag="PK502_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HPH00860.1"
                     /translation="MSTQILIPFFITAVFLVAAGITLAYMLSPKSFNLQKQEPYECGI
                     PTVGVTWLQFNVGYYTFALLFLIFDVETVFLFPWAVVMKQIGLVGLVEILIFFFILVL
                     GLAYAWKKHALVWQ"
     gene            complement(3641..4630)
                     /locus_tag="PK502_08330"
     CDS             complement(3641..4630)
                     /locus_tag="PK502_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPH00861.1"
                     /translation="MSTLVIVIVGLAFLYDFINGMNDAANSVATVVATRVLSPFVAVL
                     WAALFNFAAYFLFGLNVANTMGKGIIQPHVVEPWLVFSALLGASAWSYFCTHFGLPIS
                     VSHALIGGMVGPAWFAYGASSVVASGILNVVIFIFVSPLIGFVLGYLLMILIKHILKR
                     VKPANVDSWFRALQLLSSALFSLGHGSNDAQKTMGIIAVLLFSTGYLGSEFYVPEWVA
                     FSCYIAIALGTLTGGWKVIKTMGVNLTDLKPVHGFAAETAGAITLGMSAFMGIPVSTT
                     HTISGSIMGVGVTRRVSAVRWKVAQNIIGAWILTIPMTMIISGLLYLLIRVFV"
     gene            complement(4636..5283)
                     /locus_tag="PK502_08335"
     CDS             complement(4636..5283)
                     /locus_tag="PK502_08335"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPH00862.1"
                     /translation="MNVNSFFQFLAPKENKFYPHFNAASDNLVEVSLLLKKMMETDTL
                     DERLSFISKIKELERKGDDITHKMFDLLNGSFITPFDREDINALISSIDDVVDYINGS
                     AQQFQLYKPVSIPAEYIKMAEHIIEGSKTIQKAIYHIKELKNPVEIMDACIHVNEIEN
                     QADETYHRGIFQLFEHETNAIELIKKKDILQLLEKATDKLEDVSDVIKSILIKQS"
     gene            complement(5487..5846)
                     /locus_tag="PK502_08340"
     CDS             complement(5487..5846)
                     /locus_tag="PK502_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00863.1"
                     /translation="MEAKRFSERKLILFTVVLMIAAGLVRLVSYKVGVVLFYLSFLPF
                     IIHRLNFYHNHRGKLTDVDRRRRVTLALLLLMMLLNMVGWQNVEFILLIILAVDYMLI
                     NNRRSNGEKADDFGVKH"
     gene            6129..7892
                     /locus_tag="PK502_08345"
     CDS             6129..7892
                     /locus_tag="PK502_08345"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPH00864.1"
                     /translation="MKKYYTLLVILLLFLNATQSQPLTGTGSVANKQLLHHVSILASD
                     SMGGRFPGTTYDRISAKYIRDQLQSYGLSFQQKNGYQFFAIQTFNPNRATSTFLVVNG
                     KRLQYGNDYTALATSGNDSLCATAVFVGYGRTGGSADPDDYKNVSVKKKWAVMLVPSL
                     KVQDASSGELQQVDLSCINGAVGHEAAGIILIASEAENRFISMGSQQQALNLPVFILN
                     GKSSELILKSIGPADEVIGILERGGQNLSQDLPLTICGKAGKPYNEIETQNVVGVLRG
                     SDPNLADEYIVVGAHYDHLGMGGKEANSRMPDTVAVHNGADDNASGVAVMLEIAREMA
                     SQKKQLKRSVVFAAFGAEEMGLVGSQRFLDSAVVPIPNIKAMVNLDMVGRMVDGKLEI
                     GGTKTSMQAEAILNELNADSALAISLSPDGFGPSDHASFYSKNIPVFFFTTGIHQDYH
                     TPFDDVDKLNLTGMAQVTDYAYRLIYKLSTMDSSLTFSKSGSRMAPTNHGNKVKVKLG
                     IMPDVSGSTGNGLKVIAVSDDKPAMQAGIQSGDIITSLGGVPVTNIYDYTEQLGKLEP
                     GITVEVKVLRNNQELTFTLKL"
     gene            7918..>8646
                     /locus_tag="PK502_08350"
     CDS             7918..>8646
                     /locus_tag="PK502_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00865.1"
                     /translation="MKKALLWIAAIIVTILAAIYQRDTGPTNPKRITYTIEGTTYKAK
                     LPRSGNSTSDCKVSLNLPLTSTATLYFRRYSTQDSFSPIPFIPEDEKTQSAYLPQMPP
                     AAKLEYYIVVNEGNNPMEIMKNSPVVIRYKGDVPAWALIPHILAMFLAMLYSSLAGLA
                     AAFKMESFRKFAIITLVLLLVGGFVLGPIIQFYAFGQAWTGFPLGFDLTDNKTLIALL
                     IWLAAVIANRKHSRPKIVIAAAIVT"
BASE COUNT         2478 a         2067 c         1889 g         2212 t
ORIGIN      
        1 ccgcttcgca tggcgccaat cagcgagtac ttactattgc ttgaccatcc acccagcaaa
       61 attcccacca cacccatcga agaaaccgcc ataataaaaa acaccccaat gttcaggtca
      121 attccctgca gcccttttgc aaagggaact gtgccaaatg tgaggaacga tgcaacaata
      181 acaatgaaag gcgccaggtt aaaaagcacc ccatcagcat tttttatctt gatattctcc
      241 ttaagaagga gcttgatgaa gtcggctatg gtttggaaaa atcccttagg gccaaccctg
      301 ttgggaccca ggcgaacctg aatgaaggcg caaaccttac gctcggcgta aaccaaaaat
      361 agcccgataa gcgcataaat aaccaggtag atgatgccga taatggcata ttccaacaca
      421 gtagcccaca aatcggacat gcttgctcga aataccgcat caatactctg tattaaccca
      481 gtaaaatcat ataaaacacc ctgcatagta ttagcgttta gtgctgaatg gttaatgttt
      541 gcccataagc ccttacctgt cgatatcagg gattaccaaa tccaatgatc cgccaatggc
      601 aattaggtct gcaatttttt gatttcttga aatgaggtcc ataaccgaaa gggttgaaag
      661 gcaaggtgaa cggaacttca cccggtaggg tgttttgttg ccatcgctga taatgtgcac
      721 aacgagctcg ccacgggctg tttcaacctg ctgaaagtac tcgccttcgg gaagctttat
      781 aacagccttc attttttcca cgtagctgcc ctcggggata ttatcaataa gctgctcgat
      841 gatcttcaac gattccagca tttcatccat tctaaccttg tagcgtgcaa agctatcgcc
      901 ctcgttgtat aggatctcgt taaactgcac cttgtcgtaa gcgctgtacg gagctctctt
      961 cctcacatca caggcaaatc ccgaaccacg gccagctggg cctgtaacac cataggcaat
     1021 agcctgctcc ctggtcagaa gccctatacc catagttcga ttctgaaaaa taacgttccc
     1081 ggtaaccagc tcgtcgtact ccttgaaact tttcctgaag tagggaataa actccttaac
     1141 tcttttctgg aagttgggat gaatatcgtt cattagacct cccggacagt tgtagctgta
     1201 aagcaacctg ccgccggtgg tttcctcaaa aatatcgaga attttttccc tatcgcgaaa
     1261 tgcgtaaaag tagggggtta ccgcccccat atccatacac atggacgacc accagagcag
     1321 gtgagaggca attcggttaa gctcggcgat aatggttctg atgtacttaa tacggtctga
     1381 aacctctata tttaaagctt tttcaacagc caggcaaacc gcctcgttgt tgatgtgggc
     1441 cgaaaggtaa tccatcctat cggtcagatg gatgatctgg gggtaagaaa gggattcgca
     1501 catcttctca atacccctgt gaatataccc gcaatgcggc tgaacggatt ccacaacctc
     1561 gcccttcagc ttgaccacaa aacgcataac gccatgggtt gaaggatgct ggggacccat
     1621 gttgatttcc aaatcctgcc cctcggtacc ggcaaccgca agatgtacaa attcttctgc
     1681 cattgagcta gtattttctt tcgatgatat tactttcgtc aacataatcc tttcggaggg
     1741 ggtgccctac ccagctttcg tccaggaaca ttcggcgcat atcgggatga ttattgaagg
     1801 tgatacccat caggtcgtaa atttcacgct catggtactc ggctgtttgc cagagagagg
     1861 taacggaatc gacccgggga tttgccttat caatattcct cgtttttaaa accatgtagt
     1921 ggttgtgggt ggtggaggta agcaagtagg tgcatccaaa gctactcaca taatcaacgc
     1981 cggtaatcag gattaggtag tccatttggg tttgctcgtt ttccttaagg tttctggcca
     2041 aatcaaagag cttgtcgaac ggcacctcca cggtaaggaa ctgtgcttcc gatattacgg
     2101 cttcaacacc caagcgtttt actacttcct ctagctgctg gttggtcatg gtttgctatt
     2161 tttcgggtaa aggatttttc tcatcgtagt tgttggcaac atttttcctg gcgtacttat
     2221 tctttatggt ctccttcttg attttccgct ggagctgcat gatgccgtac agaaaagcct
     2281 cgggcctggg tgggcaaccg ggtatgtaca cgtcaacagg aataagatga tcgaccccct
     2341 tgagcacatg gtatgagttg tagaagaatg ggcctcccga aacagcacat gcccccatgg
     2401 ccacaacata cttgggttcg gccatctggt cgtagaggcg cctgagcacc ggtgccatct
     2461 tgttgacaat ggttccggct accacaataa cgtcggcctg acgcggcgat gccctgtaaa
     2521 cctcaatgcc aaagcgggca aaatcgtggc gtgctgccgc cgtggccatc atctcgattc
     2581 cgcaacagct ggtggcaaag gtgagcggcc atattgaatt ggaccgaccc cagttaagta
     2641 tgctgtcaac agtagtaaaa aagattccat tctcctcgag catttgctga tctaaggtat
     2701 cggtatcctt aaaatcagac tccttcattg attttattgc cataccaaag cgtgtttttt
     2761 ccaggcgtag gctaaaccca acaccaggat aaaaaagaat atcaggattt caaccagacc
     2821 taccaaccct atttgcttca ttaccactgc ccagggaaac aggaaaactg tttccacatc
     2881 gaatatcagg aagagcagcg caaaggtgta gtacccaaca ttaaactgga gccacgtaac
     2941 cccaaccgtg ggaattccac actcgtaggg ctcttgtttt tgcaggttaa acgatttggg
     3001 cgacaacatg taggccagtg tgattccggc agccaccaga aaaacggctg taatgaaaaa
     3061 gggaattaaa atttgagtgc tcatgccatt aatgtatgag gttgttcagc aaatttatcc
     3121 gagtagtaaa tagtaaattg agttgctatc aatcttaaat atttcaggat ggagacccaa
     3181 cacatttttt ttgcaaccac aaaagtagat tttttgggta tatgatttaa ctaaaattca
     3241 cattttttta tttttatatc tatctattaa cataactgga taacaaaaat aagtttttaa
     3301 cgatgtaaga gcttagccta agatttgctc tctattgcga aaggttttca atactccctt
     3361 ctgcattgga gacttaatgt aaaaccatca agcttactca atggatatat ggtgggtagt
     3421 ttaggaagta aagtgaaaaa caaccattct ttaaatgttc tacaagaatt gcattagcct
     3481 atttataacc aggcttagct aaatatttta gccataaact gcaaacagtt ttcttccaaa
     3541 tctggagagc atacggctag cttagggtag tacaataaaa catgattggt ttgaaaatga
     3601 gcaaaccaaa atagctttca taaaaacttt caagagcctg ttacacgaaa acccgaatga
     3661 gcaggtagag caatccggaa atgatcatcg tcattggaat ggtaagtatc caagcaccta
     3721 tgatgttttg agccaccttc caccggacag ccgagaccct gcgtgtaacc ccaactccca
     3781 tgattgagcc ggaaattgtg tgggttgtgc tcacgggaat gcccataaag gcagacattc
     3841 caagggttat tgcaccggcg gtttctgcag caaagccatg aaccggctta agatcggtta
     3901 ggttaacccc catcgtttta atcaccttcc aaccgccagt taatgtgccc agcgcaatgg
     3961 cgatgtaaca tgaaaaagcc acccattcgg gaacataaaa ttcggagccg agataccccg
     4021 tgctgaaaag gaggactgca ataataccca ttgttttttg tgcatcgttg ctaccatgcc
     4081 caaggctgaa aagggcagac gataaaagtt gaagcgccct gaaccaggag tccacatttg
     4141 caggtttaac ccttttgagg atatgcttaa tcaggatcat caaaagatat ccaagaacaa
     4201 aacctataag gggtgaaaca aaaatgaaga taaccacatt gagtattccc gaagccacaa
     4261 cgctactagc cccataggca aaccacgccg gacctaccat ccccccaatt aaggcgtgtg
     4321 aaacgcttat tggtaggcca aaatgggtac agaagtacga ccatgcagat gcaccaagga
     4381 gagccgagaa aacgagccat ggttcaacaa catggggctg aataatacct ttgcccatgg
     4441 tgttggcaac gttgagccca aacaaaaagt aagcggcaaa gttaaacaat gctgcccaaa
     4501 gcacagccac aaaaggcgat aacaccctgg tggcaacaac ggtagcaact gagtttgcgg
     4561 catcattcat gccgtttata aaatcataca aaaaggctaa tccgacgata acaattacta
     4621 gggtgctcat gctggctatg attgcttgat taaaatggac ttgataacgt cggaaacatc
     4681 ctctagctta tcggtagctt tttcgagcag ctgcaatata tctttcttct taatcagctc
     4741 aatggcattg gtttcgtgtt caaaaagctg gaatattccc cggtggtatg tttcgtcggc
     4801 ctgattttct atctcgttaa catgaatgca ggcatccata atctctaccg ggtttttcag
     4861 ctccttaata tggtaaattg ccttttgtat ggttttgctc ccttcaatta tatgctcggc
     4921 catcttgatg tattccgcag gaatcgatac aggtttgtac agttggaatt gctgcgcaga
     4981 accgtttatg tagtctacca catcgtcaat ggaagagata agggcgttga tatcctcccg
     5041 gtcaaatggt gtgataaacg atccgttaag tagatcgaac attttatgcg taatatcgtc
     5101 ccctttcctt tcaagctcct taattttact gataaacgac aagcgttcat ctaatgtatc
     5161 ggtctccatc attttcttta aaagcagcga aacttcaacc aggttatccg acgctgcatt
     5221 aaaatgagga tagaatttgt tttccttggg ggcaagaaat tggaaaaaac tgttcacatt
     5281 catctttctg aaatatgtta agtttatatg tttgattttc aattataaag aaacacaaaa
     5341 ataagctatg agaatctttc caatgttaag taattgttaa gttaacttta actgcttggt
     5401 attaagcaaa tatgttgata atcaaatatt acataatttt tttggttaac caagtgctgg
     5461 aagcaaataa tgaaccctca agttgtttaa tgctttaccc caaaatcatc ggccttttca
     5521 ccattactcc tacggttatt aatcagcata tagtccactg ccaggatgat aagcaggatg
     5581 aattctacgt tttgccatcc aaccatgtta agaagcatca tcagcagcag aagggctaac
     5641 gtaacccgcc ggcgccggtc aacatcggta agtttacccc tgtggttatg gtaaaagttt
     5701 aatctgtgaa taataaaagg caggaaggac aggtagaaca gaacaacacc caccttgtag
     5761 ctaaccagcc taaccagtcc ggctgcaatc atcagcacca cggtaaaaag gattagctta
     5821 cgctccgaaa atcttttagc ctccataacc taattagaag taatcagaat ttgggattca
     5881 atgcagctgc ccggctgatg aaaaaccaac agcttttgaa cccgcccccg caccgcccgt
     5941 caatatgttt agcaagcaaa aatagatatt ttttggtcaa ccagccggaa tggctaacgg
     6001 tgttttggca atcccgacta gttaccgtgg cacttaaatt gcaggccgtg aacacttact
     6061 gattttattt catatatcac tatatttgac ggttgaattt ataacggggt aactaccaaa
     6121 caataaacat gaaaaaatat tacacgctgc tggtaatctt acttttattt ttaaacgcta
     6181 cccaatcgca acctctaaca ggaaccggta gtgtagcaaa taagcaactt ttacatcatg
     6241 taagtatttt ggcttcagac agtatgggag gacggtttcc tggcacaacc tacgacagga
     6301 tttcggccaa gtacatccgt gaccagcttc agagctacgg tctctccttt caacaaaaaa
     6361 acggctacca attctttgcc atccaaacct ttaacccaaa tagggcaaca agcactttcc
     6421 tggtggttaa tggcaaaaga cttcaatacg gcaacgacta cactgccctt gccacaagcg
     6481 gaaacgattc gctttgtgct acggcagttt ttgtaggcta cggccgcacg ggcgggtctg
     6541 ccgacccaga tgactataaa aatgtttcgg tgaagaaaaa gtgggcagtt atgcttgtcc
     6601 ccagcttgaa ggtgcaggat gcctccagcg gtgaacttca acaggttgac ctcagctgca
     6661 tcaatggggc tgtagggcac gaagcagcgg gcataatcct gatagccagc gaagctgaaa
     6721 atcgcttcat ttccatggga agtcagcagc aagctctcaa cctgccggtt tttattctaa
     6781 atgggaaatc atcagaattg atacttaaga gtattggccc agctgacgag gtgatcggaa
     6841 tactggaaag gggtggacaa aatttgtcgc aggatttgcc ccttaccatt tgcggaaaag
     6901 caggcaaacc ctacaatgag atagaaaccc aaaacgttgt aggtgtcctg agaggaagcg
     6961 accccaacct ggctgacgag tacattgtgg taggtgccca ctacgaccac ttgggcatgg
     7021 ggggcaagga ggccaacagc cggatgcccg atacggttgc ggtgcacaac ggagccgatg
     7081 ataatgcctc gggagtggct gtcatgctgg aaatagcacg agaaatggcc tcacaaaaaa
     7141 aacagcttaa acgcagcgtg gtttttgctg cctttggagc cgaggagatg gggcttgtcg
     7201 ggtcgcagcg cttccttgac tcggcggttg ttcccatccc gaacatcaag gccatggtaa
     7261 acctcgacat ggtaggcaga atggttgatg gtaagctgga aattggcggc actaaaacct
     7321 caatgcaagc cgaagcaatc ctgaacgagc tgaatgcgga tagtgcgctg gccatttcgc
     7381 tatcgcccga tggttttggc ccctccgacc acgcttcgtt ttactccaaa aacattccgg
     7441 ttttcttttt caccacaggc attcaccagg actaccacac gcctttcgac gatgttgaca
     7501 agctgaacct tacagggatg gcgcaggtaa ccgattacgc ctaccggcta atctataagc
     7561 tatccaccat ggacagctcg ctcaccttca gcaagtcggg tagccggatg gcacccacaa
     7621 accacggcaa caaggttaag gtaaaactgg gtattatgcc cgatgtgagc ggttccacag
     7681 gaaatgggct taaggttata gccgtttccg atgataaacc ggcaatgcag gctggcattc
     7741 aaagcggtga tatcatcacc tctttgggcg gagttcctgt tactaacatc tacgactaca
     7801 ccgaacagct gggcaagctg gaacccggca tcacggtaga ggtaaaagtt cttagaaata
     7861 accaggagct gacgttcacg cttaaacttt agaaactaca aactaaaaca taccgaaatg
     7921 aagaaagccc ttctctggat agcagccatc attgtaacca tcctggcggc tatttaccaa
     7981 agggacaccg gccccaccaa ccccaaaaga attacctaca ccatcgaggg aaccacctac
     8041 aaggcaaaac ttccgcgcag tggcaacagc acatccgact gcaaggttag cctaaacctg
     8101 ccgcttacct caaccgcaac cctatacttc aggcgttact ccacacagga tagcttctcg
     8161 cccatccctt tcatccctga agatgaaaag acacaaagcg catacctgcc ccaaatgccg
     8221 cccgctgcca aacttgaata ctatattgtg gtaaacgaag gtaacaaccc catggaaatt
     8281 atgaaaaata gcccggtggt tataaggtac aagggtgatg tacctgcatg ggcgcttatc
     8341 cctcatatcc tggccatgtt cctggctatg ctctactcca gcctggcagg gttggcagct
     8401 gccttcaaaa tggaatcctt caggaaattc gccattatta ccctggtgct tttgctggtt
     8461 ggaggctttg ttctgggacc aattatccag ttctacgcct ttggacaggc atggacgggc
     8521 tttccccttg gatttgacct gaccgataac aaaactctca tcgccctgct aatttggctt
     8581 gctgcggtta tagccaaccg caagcactcc cgacccaaga tagtaattgc tgcagcaatt
     8641 gttacg
//
LOCUS       DAOPHX010000109         8573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_10380_length_8573_cov_4.595328, whole genome shotgun sequence.
ACCESSION   DAOPHX010000109 DAOPHX010000000
VERSION     DAOPHX010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8573
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10380_length_8573_cov_4.595328"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..653
                     /locus_tag="PK502_08355"
     CDS             129..653
                     /locus_tag="PK502_08355"
                     /inference="COORDINATES: protein motif:HMM:NF024954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPH00866.1"
                     /translation="MGRKSLQVQGYSSEEIRALFTKDERYTIGIRLYAVYQVSLGQAS
                     RKLESLYNTSFKQITNWVHRFEEFGLEGLKDKPKSGRKPRLAEEQLNELSSVLQNNRP
                     DEFGYNTATWNGPILKDYIEKHYHVAYQKAQVYNLLKKLGFTYQKGRAQYPEADEGKR
                     EEFKTTLKKTKRGT"
     gene            664..1176
                     /locus_tag="PK502_08360"
     CDS             664..1176
                     /locus_tag="PK502_08360"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HPH00867.1"
                     /translation="MLFEDEFSLSNTATISYSWAVRGKQPRVVSKQNRRERQTTFGSI
                     NIRTGQMVINFSDKGNFKSFKKHLKKILHTFKSAPKIILVLDNVRYHHAKLLKKFLEE
                     NQRLEILYLPPYSPDLSPIERAWWFMRKSVTHNRFMESLKLRKAKFWQMFSPFQVPNE
                     KILTICAIIY"
     gene            complement(1296..1907)
                     /locus_tag="PK502_08365"
     CDS             complement(1296..1907)
                     /locus_tag="PK502_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPH00868.1"
                     /translation="MISVQIVDDHRILVDGMLRLINESGVAQVTGTAYCGKEALDMLR
                     LKEPQVLLLDINLPDANGVDLCATIKEKYPNVKVIALTSFGEYTVVRRMLEAGANGYV
                     LKNAMAEEVLDGIECVASGDTFLCDEVDLLMKKQSSSAIWLTPRERELLKLIVEGYTN
                     PEIADKIFLGVETVNSYRKNLLFKLNARNTAVLVKMAIEQKLI"
     gene            complement(1919..3859)
                     /locus_tag="PK502_08370"
     CDS             complement(1919..3859)
                     /locus_tag="PK502_08370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPH00869.1"
                     /translation="MEKNCLKKVMLVTILAIASSLPLSAQDDNQVNVDSMLNSAKSKL
                     YSSPNEAFELAQQVVELSVEQGNQLLLANGYKLLGAYYSDIKNDFDLAKLNYQKADSI
                     FRHNKGEEYAKGIGAIYHSYGVIDHRLGNYWEAIQYYYNALRVLDSINDNEIRPKTLN
                     NLSNLYAFLKDYSRAEKYSRECLRVSIENGNKQMISVVSVSLADALISQGKFEEVLSL
                     LLKSKEIATEQENLYILELVHLNLGSYYYLSENNYEKSIGEYLQALNYAKALGSDWEV
                     MRITTNVSEVYFLDNQLGKAMEYAEKSLGIAERMGTGDIKQRALSILAKVYASKGQYS
                     NAYENLLKSYIIRDSVFNDSKQMHVNYLESRYHSEMKEKEIIILKNTQREHELILKRR
                     KYQLIILITIILIVALVAAFIIVRNKYRQLLDAQKLHIQEQQIKQLEQEKQLVATQAI
                     LEGEATERKRLARDLHDGLGGMLSVVKMGLHDVKSGVALDSDDVLRFNKVLEMLDSSI
                     RELRRVAHNMMPESLMRYGLKASLTDFCGNIPNVQFHYFGNEQRLDSKLEMMIYRTAH
                     ELVNNALKHAQAQHINIQIVQGTDRVSLIVQDDGVGFDPKVKSEGTGLDNIRCRVQLF
                     NGTMDIFSKTGNGTEVNVEFKL"
     gene            4217..4792
                     /locus_tag="PK502_08375"
     CDS             4217..4792
                     /locus_tag="PK502_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00870.1"
                     /translation="MKQLTTMKRITFLLVAMCFTTAMASAQNYFTKGAKVINATVGFG
                     NSLYTGSGYTGKVPPIAASFEVGILDNVFDEKSSIGVGGYLGYTSAKWESGGYGWRYS
                     DFIIGVRGSFHYQLIDKMDTYTGLLLGYDIVRATEIGDHTLWSGSSASSSGLAWSWYV
                     GGRYYFNDKFAGLVELGYGIAYFNIGLSIKF"
     gene            4845..6524
                     /locus_tag="PK502_08380"
     CDS             4845..6524
                     /locus_tag="PK502_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPH00871.1"
                     /translation="MKKLHLLLALLALLFVMPSCETEDETEVTVITGTISTPITWKAG
                     ETWVVEGSVYLEADLIIEPGTTVEFKKDAVVYVGYSAPASITALGTADKPIVFRGYEG
                     QTWKGIELYAHNSATKSAFTYCTFKQLGGDYALLIYGTKAKLDHCSFTECAKSAIHVL
                     GGGDFNGFTNNTLQDCGAFLMEIDADVAGSLGTTNTFTATGNKGVRISGSRNVTRAAT
                     WSSIGAPYVIANEQYIQVDADLTIAAGTTVLFEPHSMLEVGYSEYASLKAVGTAQAPI
                     TFTSASANPQPGDWYGIQFYSKNSSTTSELTFCTIEYAGTQSYPALFIGDTQVKLNDC
                     TVAKGQYYGVEVDGNAKFTQCMRNTIKEFGNHPMRVDVNAVYTIDASNTILSDGNFGV
                     YIPNYDLTGTVTWNKHEVPYFVEFIRFNNNAVLTIAPGTTVKFMAEGYFEVGYDAYGK
                     LIAEGTANDPITFTSAASSPSAGDWGGLQFYSQTSAGTKLNNCLVAYAGYPGYDTGAI
                     YINECGSAISLANTQIAHSNACDVYVYYSATPAMVNVTNHNGEQANVCVVN"
     gene            complement(6736..7428)
                     /locus_tag="PK502_08385"
     CDS             complement(6736..7428)
                     /locus_tag="PK502_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HPH00872.1"
                     /translation="MRQHYVSLCIELYGQQEREKAKKFKTYEPGYLHIDVTYCPKLDG
                     VKWYLFVAIDRATRLILYELYDAKTAENAEDFIHKCLAFFPFQITHILTDNGLEFTNA
                     LLKSKNGKPCAKPSRVDELCKKENIKHRLAKPHTPKTNGMVERANGIIKSSTIPKEQY
                     ANKQSMEAHLLEFLVFNILYRRHGSLRRELGVKTPYEAVEKPLVFKNKVVKWQRHFKS
                     KQGSFIQQPCET"
     gene            complement(7355..7702)
                     /locus_tag="PK502_08390"
     CDS             complement(7355..7702)
                     /locus_tag="PK502_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00873.1"
                     /translation="MQGYHSNATTNVHIRCELQKSNLPMNIPADQYQISKNTVFKWKN
                     RNGQDDRSSRPHTIHYYLSALEKEIINSARRSSWLPLEEITEVAQQANPAALRIALYR
                     TLRAAGEGKGKKV"
     gene            7852..>8573
                     /locus_tag="PK502_08395"
     CDS             7852..>8573
                     /locus_tag="PK502_08395"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPH00874.1"
                     /translation="MKLRVGNLLLAMMLMALPSSPCTIIAVGKKASADGSNIISHTDA
                     GPDSRIFVVPAKSFKPGTMAPVYWGIQDADRPLNDDGIILGYIPQVEKTYKYFQSAYS
                     HVNEYQLGIAESTTDQRRELFCNRDNGKQIMTIEQAQIFALQRYKTAREAVRFIGSLM
                     TRYGFLPSSGDGSETLVIADTEEIWVLEVFGVGPGWTPGSGKPGAIWAAQRLPEDEAT
                     IVPNWSIIKEIDASDTLNFMVS"
BASE COUNT         2461 a         1991 c         1803 g         2318 t
ORIGIN      
        1 gataaatcaa attcaagaat ttcaggaatt gcttccttat agccgggtct gaaattttta
       61 atatataaag tctaatttgt aattacacat taaatgtagt aattttgtaa aaactaaatt
      121 actacattat gggaagaaaa tcattacaag ttcagggcta tagttcagaa gaaattagag
      181 ctctgtttac taaggatgaa agatatacca tcggcatcag gctgtacgcg gtttatcagg
      241 tttcacttgg gcaagccagt cgcaaattag aaagcttata caataccagt tttaagcaaa
      301 tcaccaactg ggtacatcga tttgaagaat ttggattaga ggggctgaag gacaaaccca
      361 aaagcggccg taagccaagg ttagctgaag agcagttgaa tgagctgagt agtgtgttgc
      421 aaaataatag acccgacgag tttggctaca atacagctac atggaatggg cctatactaa
      481 aagattacat tgaaaaacac tatcatgtag cataccaaaa agcgcaggtt tacaacttgc
      541 tgaagaagct tggttttacc tatcaaaagg gcagagccca gtatcccgaa gcggatgagg
      601 ggaaaagaga ggaatttaaa acgacattaa aaaaaactaa aagaggaacc tgatagcacc
      661 gttattctat ttgaggatga gtttagctta agcaacacgg caacgatatc atacagctgg
      721 gcggttcgtg gaaagcaacc cagggtagtg tcaaagcaaa acagaagaga gcgccaaaca
      781 acttttggca gcataaacat aaggacaggg cagatggtca ttaacttttc ggataaaggc
      841 aacttcaaga gcttcaaaaa acatttgaag aaaatactgc acacattcaa aagtgctcca
      901 aagattattt tggtgcttga caacgtaagg tatcaccatg cgaagcttct aaaaaagttt
      961 ttggaggaaa accaaaggtt ggaaatcctt tatttgcctc catattcacc agatttaagt
     1021 ccaattgaaa gggcttggtg gttcatgaga aaatcggtaa cccacaatag gtttatggaa
     1081 tctctgaagt tgagaaaagc aaaattttgg cagatgtttt ctccttttca agttccaaac
     1141 gaaaaaatat taactatatg tgcaattatt tattagactt tatataaaat gcgagctaat
     1201 gtgtgcgtgg tgaactaaga acgaacaaaa aaacgagaag ccccattgta gcaactacaa
     1261 tggggcttct gcatgggaaa ggaaatgagt aataactata tcaacttttg ctcaatggcc
     1321 attttaacca gcaccgcagt atttcgggca tttagcttaa aaagaaggtt cttacgatag
     1381 ctattcaccg tttctacgcc aagaaaaatc ttgtcggcaa tttcggggtt ggtatagccc
     1441 tccacaataa gctttagcag ttcgcgttca cgaggggtta accaaatcgc gctgctcgac
     1501 tgctttttca ttaaaaggtc aacctcatcg caaagaaagg tatcgccact ggccacgcac
     1561 tcaataccat ctaacacctc ctcggccatt gcgtttttta gcacataacc gtttgcaccc
     1621 gcctcaagca tgcgtcgtac tacagtgtac tcgccaaaac tagtaagtgc aataacctta
     1681 acattaggat atttctcttt gatggttgcg caaagatcaa ctccattggc atcgggaagg
     1741 ttgatatcca gcaaaagaac ttgaggctcc tttaggcgaa gcatgtccag agcctccttg
     1801 ccacaatagg ctgtgccggt tacctgtgca acacccgact cgttaatcag cctaagcatt
     1861 ccatcaacca gaatacgatg gtcgtccact atctgtacgc ttatcatgcc aatatcccct
     1921 ataatttaaa ctcaacgttt acctctgtgc cattaccagt ctttgaaaaa atatccatag
     1981 ttccgttaaa caattgcacc cggcaacgga tattgtcaag ccctgtgccc tcggatttta
     2041 cttttgggtc gaacccaacc ccatcgtcct gcacgattaa cgaaacccta tctgttccct
     2101 gaactatttg gatattgata tgctgcgcct gagcatgttt taaagcgtta ttcacaagtt
     2161 catgagcggt gcggtaaatc atcatctcca gcttgctatc aagccgttgc tcgtttccaa
     2221 agtaatggaa ctgcacgttg ggtatattgc cacagaaatc ggttagcgac gcctttaaac
     2281 cgtagcgcat cagcgattca ggcatcatgt tatgcgctac acgccgtaac tcgcgaatgg
     2341 agctatccag catttcgagc actttattaa agcgcaacac atcgtcgcta tcaagggcaa
     2401 caccactttt tacatcgtgc aaacccatct taacaacgga gagcatccct cctagcccat
     2461 cgtgcaaatc gcgagccaag cgcttacgct ccgttgcctc gccttccaga atggcctgcg
     2521 tggcaaccag ctgcttctcc tgctctagct gtttaatttg ttgttcttga atatgaagtt
     2581 tttgtgcatc tagcaactgt cgatacttat ttctaacaat aataaacgca gcaactaagg
     2641 cgactattaa tattattgta atgaggatta ttaattgata ttttcttctt ttcaatataa
     2701 gttcatgttc tcgttgggta ttcttaagga ttattatttc cttttctttc atctcagaat
     2761 ggtaacggga ttctaaataa tttacgtgca tttgtttact gtcattaaat acactatccc
     2821 taataatata ggactttagt agattttcgt acgcattaga atattgccct ttagaagcat
     2881 aaaccttcgc caaaatcgat aaagcccttt gctttatatc tcctgtcccc attctttcgg
     2941 ctattccaag agatttttca gcatattcca ttgcttttcc aagttgattg tctagaaaat
     3001 agacttccga aacattggtg gttatgcgca tcacttccca atcactacct aatgcctttg
     3061 cataatttag agcctgaagg tactctccta tagatttttc atagttattc tcacttaaat
     3121 aataataact acccaaattt aaatgaacca actccaatat gtaaagattt tcctgttcgg
     3181 tagcaatttc ctttgatttg agcaacaaac taagaacttc ttcaaatttt ccctgagaaa
     3241 tcaatgcatc agccaaactc acgctaacaa cagatatcat ctgcttatta ccgttttcta
     3301 tactaactct taaacactcc cttgaatatt tttctgccct actataatcc tttaaaaaag
     3361 cataaaggtt tgacaggtta tttagggttt taggtcgaat ttcattatcg ttaatgctgt
     3421 ccaaaaccct aagcgcatta taataatact gaattgcctc ccagtaatta cctaagcgat
     3481 gatcaataac accataacta tgatagattg cccctatacc ctttgcgtat tcctccccct
     3541 tattgtgcct gaaaatacta tcagcctttt gataatttaa ctttgctaaa tcgaaatcat
     3601 ttttaatatc ggaataatag gcacctaata acttatatcc attagccaat aacaattgat
     3661 ttccctgctc aacagacaac tcaactactt gctgcgctag ttcaaaagcc tcattgggag
     3721 aagagtaaag ttttgatttt gccgagttca acattgaatc gacattaacc tgattgtcat
     3781 cttgagcact taaaggtaaa gaactcgcta tagccaaaat ggtaacaagc ataacctttt
     3841 ttaaacaatt cttttccata ctcttatgct actttattat tggtcttttt gtggggcgaa
     3901 ataaaaatta gccacgtcat aaaaaatcat catacgccag acaactctaa tttaggtaaa
     3961 agaaatatac cattaagaaa aacaggagca attctaaaat aagaataaag tatttggctt
     4021 atgcaaaaag ccttcataag aatcacacat atatctctcc atacctataa ataacgttga
     4081 ctatcccaat gcaaatttac atcttacatc gtcaaaaaaa tacccccaaa agggggtagt
     4141 gtaaaaaaac atagccacct acttttgcat gagaaaagaa actcacacaa gagttctgaa
     4201 acaaaaatta actactatga aacaattaac tactatgaaa cgcattacct ttttgctagt
     4261 tgcaatgtgc tttaccacag caatggcaag cgcgcagaat tactttacaa agggagcaaa
     4321 ggttattaat gccaccgttg gctttggtaa ctcgctctac acaggaagcg gctacacagg
     4381 caaagttcca cctattgcag catcgtttga agtgggtatt ctcgacaatg tgttcgatga
     4441 gaaaagctcc attggtgttg gcggatacct aggttacact tccgctaagt gggaatcggg
     4501 tggatacggc tggcgctact ccgactttat tattggtgta agaggctcat ttcactacca
     4561 gcttattgat aaaatggata catacacagg cctactactt ggctatgaca ttgttagggc
     4621 taccgaaatt ggcgaccaca cgctttggag tggcagttcg gcctcgtcga gcggtttagc
     4681 gtggtcctgg tacgtgggtg gacgctacta ctttaacgac aaatttgctg gccttgtaga
     4741 gctaggttac ggtattgcct acttcaatat aggattatcg ataaagttct aaggctcatt
     4801 cttttaaaac aaaaacgtta attccaactg ctaaaatcaa aaaaatgaaa aagttacact
     4861 tactactagc actactggca ctactatttg taatgccatc gtgtgagaca gaagacgaaa
     4921 ccgaggtaac cgttataacc ggaaccatat cgacccccat tacatggaaa gcaggggaga
     4981 catgggtggt tgagggcagc gtttacctgg aggccgacct gattattgag cccggtacca
     5041 ccgtggagtt caagaaggac gccgtagtgt acgtgggcta ctccgcaccg gccagcatca
     5101 ccgcgctggg cactgccgat aagcccattg tgttcagggg ctacgagggc caaacttgga
     5161 agggcattga gctctacgcc cacaacagcg ccacaaaatc cgcctttacc tactgcacct
     5221 ttaagcagct gggcggggac tacgccctgc tcatctacgg gactaaggcc aagctggacc
     5281 actgctcctt taccgagtgc gccaaatcgg ccatccacgt gctgggcggt ggcgacttca
     5341 acgggttcac caacaacacc ctgcaggatt gcggagcctt ccttatggag attgacgccg
     5401 atgtggcggg ctctctgggc accaccaaca cctttaccgc tacggggaac aagggcgtgc
     5461 gcattagcgg gagccgcaac gtaacccgtg ccgctacgtg gagcagcatt ggcgcgccct
     5521 acgttattgc caacgagcag tacattcagg tggacgccga cctgaccatt gccgctggaa
     5581 ccaccgtgct ctttgagccc cacagcatgc tggaggtggg ctacagcgag tacgcttcgc
     5641 ttaaggccgt gggaacagcc caggcgccca tcacctttac ctcagcatcg gccaacccac
     5701 agccgggcga ctggtacggc atccagttct actccaagaa ctcgtccacc acatcggagc
     5761 tcaccttctg caccattgag tacgcgggaa cccagtcata ccctgccctg ttcatcggcg
     5821 acacccaggt taagctcaac gactgcaccg ttgccaaggg ccagtactac ggcgtggagg
     5881 tggacggcaa cgccaagttc acccagtgca tgcgcaacac cattaaggag tttggcaacc
     5941 accccatgcg cgtggacgtg aacgcggtgt acaccataga cgcatccaac accattctct
     6001 ccgatggtaa ctttggcgtg tacatcccca actacgacct taccggaacc gtgacctgga
     6061 acaagcatga ggtgccctac tttgtggagt tcatccgctt taacaacaac gcggtgctaa
     6121 ccattgcccc gggaaccacc gttaagttca tggccgaggg ctactttgag gtgggctacg
     6181 atgcctacgg caagctcatt gccgagggta ccgccaacga tcccataacc tttacttcgg
     6241 ctgcgtccag cccttcggca ggcgactggg gcggactcca gttctacagc caaaccagcg
     6301 cgggaaccaa gctcaacaac tgcctggtgg cctacgctgg ctaccccggc tacgacaccg
     6361 gggccattta cataaacgag tgcggtagcg ctatatcact agccaacacc cagattgcac
     6421 acagcaatgc atgcgacgtg tacgtgtact actccgctac acctgctatg gttaacgtga
     6481 ccaaccacaa cggggagcag gctaatgtgt gcgtggtgaa ctaagaacga acaaaaaaat
     6541 gaaagagaag agggtgtcca aaaaataaag gacaccctct tttcgttccg acacgtacct
     6601 gagttaacct tcaaagactc aatccacaaa aaaataaact gcctttctag atgcctctta
     6661 ttgttagcct caagtgaata attacaatgc aacttatccc ctcgtaaggt taactctctt
     6721 aagatagaat acttgtcaag tttcacaagg ttgttggata aagctgcctt gctttgactt
     6781 aaaatgacgc tgccatttta cgactttatt tttaaacacc aagggttttt ctactgcttc
     6841 gtaaggcgtt ttcacaccga gctcccttct caaacttccg tgtcgtctgt aaagaatatt
     6901 gaagacgagg aactccagga ggtgtgcttc catgctttgc ttgttggcat attgctcttt
     6961 ggggatggtg cttgatttga tgatgccgtt agctctttcc accatgccgt ttgttttggg
     7021 tgtatgtggt tttgcgagac gatgcttaat gttttccttt ttgcatagct catccaccct
     7081 ggaaggttta gcacatggtt ttccgttttt agatttgaga agggcattgg taaattcaag
     7141 tccattgtcg gttaaaatgt gcgtgatttg aaaagggaaa aacgccaggc atttgtgaat
     7201 aaaatcctca gcattttcag cagttttagc atcatagagc tcatacaaga taagtcgggt
     7261 agccctgtca atagccacga ataaatacca tttcacgcca tctaatttag ggcaataggt
     7321 tacatctatg tgcaggtaac ctggttcata cgttttaaac ttttttgcct tttccctctc
     7381 ctgctgcccg taaagttcta tacagagcga tacgtaatgc tgccggattc gcctgctgtg
     7441 ccacttccgt aatttcttcc aacggtagcc atgaacttct gcgggcggag ttaataattt
     7501 ccttttccag agcggaaagg tagtaatgaa tggtgtgagg tcgggaactt ctgtcgtctt
     7561 gtccattgcg gtttttccac ttaaagaccg tgtttttgga aatctgatac tgatctgccg
     7621 gtatgttcat tggcaaatta cttttttgca gctcgcaacg aatgtgtaca tttgtcgttg
     7681 cgttggaatg atacccttgc ataggttgta aatttttatg gttaataatt tacaaagcaa
     7741 ggtgtttttc ccgagttaag ttttcccgcc ctgcagggcg ggaaaactta accttcacat
     7801 ccaacaacgt ggtgaaactt tacagttact aacccaaaat ttacgattaa aatgaaatta
     7861 agagtaggaa acttgctcct ggcaatgatg cttatggcat tgccatcgag tccctgcacc
     7921 ataattgcag tgggtaaaaa ggcctcggcc gatggttcaa acatcatctc gcacaccgat
     7981 gcgggacccg actcccggat ttttgtagtt ccggccaaat cctttaagcc tggaacaatg
     8041 gctcctgttt actggggaat tcaggatgcc gacaggcctc taaacgacga tggaataata
     8101 ctggggtaca ttccgcaggt tgaaaaaact tacaaatact ttcaatcggc ctactcacat
     8161 gttaacgagt accagcttgg gatagccgaa agcactaccg accagcgcag ggagctgttc
     8221 tgcaaccggg ataacggtaa gcagataatg actattgagc aggcccagat atttgccctt
     8281 cagcgctaca aaaccgcacg ggaggcggtg cgattcattg gcagcctgat gactcggtac
     8341 gggtttcttc catctagtgg agatggatcc gaaaccttgg taattgcaga taccgaggag
     8401 atttgggtgc ttgaggtttt tggcgttggc cctgggtgga cgcccggcag cggaaagcct
     8461 ggtgctatct gggcagccca aaggcttccc gaagatgagg ccaccattgt tcctaactgg
     8521 agcattatca aggaaattga tgccagcgac acgctaaact tcatggtttc gga
//
LOCUS       DAOPHX010000110         8542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_10440_length_8542_cov_5.237658, whole genome shotgun sequence.
ACCESSION   DAOPHX010000110 DAOPHX010000000
VERSION     DAOPHX010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 8542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8542
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10440_length_8542_cov_5.237658"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PK502_08400"
     CDS             <1..372
                     /locus_tag="PK502_08400"
                     /inference="COORDINATES: protein motif:HMM:NF015419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00875.1"
                     /translation="VQPGKPTTFGSLDNKLVFGLPGNPVSAFIQFELLVKPAIRKLMG
                     CKQLNPTAIRLPLAEDYRRNRAERMALVPVKIIAGSAALPVEFHGSAHIFALGNADGI
                     IAVPIGTTKIDKGEIVDVRPL"
     gene            335..1156
                     /locus_tag="PK502_08405"
     CDS             335..1156
                     /locus_tag="PK502_08405"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPH00876.1"
                     /translation="MIREKLLMYDRFNRQINYLRISVTDRCNLRCTYCMPEEGITQLD
                     HSEILSFEEIASFTRIAATKGFNKVRITGGEPLVRKGVVELVSMISNIEGITDLSMTT
                     NGVLLGKYAKELKYAGLQRVNVSLDTVNPTRYRQITRVGSLDDVLNGLECARAAGLTP
                     IKINCVVDKSSAEPDAVEVKKFAQANGFEVRFIPKMDLNAGIFGEVEGGNGGNCSICN
                     RLRLTANGFVKPCLFSNLGYSVRGLGYEGALLKAVNAKPARGTTNHDGKFYNIGG"
     gene            1158..1613
                     /gene="moaC"
                     /locus_tag="PK502_08410"
     CDS             1158..1613
                     /gene="moaC"
                     /locus_tag="PK502_08410"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HPH00877.1"
                     /translation="MELSHTDRDGRANMVDVGSKPNQLREARAKGFIRLQPETLKLIT
                     ENQVKKGDVLTLAEIAGIQAAKQTPTLIPLCHTILLAKVDVKASIVQNGIEISSYIKC
                     IGQTGVEMEALTAVSVALLAVYDMCKAVDKEMTIESIKLTEKIKHDINS"
     gene            1625..2560
                     /locus_tag="PK502_08415"
     CDS             1625..2560
                     /locus_tag="PK502_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HPH00878.1"
                     /translation="MDTSVKVLSVNVSDAKGTAKHPVDFIELNSTGVKGDAHSGMWHR
                     QVSLLGVESIEKFNAANNLRIKYGDFAENITTKGFCLYECIPGDRLVGSGVELEVSQI
                     GKLCHADGCAIFSQVGSCIMPREGIFARVLKTGRLKAGDELELIPRTYKVKVVTLSDR
                     ASRGDYEDRSGPEVVRLVNQFFNDIGWRHSVNSTIIPDDAATLTSTVVEAKNNGYDLL
                     ITTGGTGIGPRDVAPDVVRPLLDKEIPGIMEMIRMKYGADKPNALISRSIAGVMGSMF
                     VYTLPGSVKAVGEYMQEIIKIQKHLFLMLMGIDSH"
     assembly_gap    2863..2962
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3519..3707)
                     /locus_tag="PK502_08420"
     CDS             complement(3519..3707)
                     /locus_tag="PK502_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00879.1"
                     /translation="MPVRSFWLLLLARCKFLHTPVREEVLANGRGVAGGCKSGKSHKQ
                     GQRADAQKTDRRLTSDLL"
     gene            complement(3753..5804)
                     /locus_tag="PK502_08425"
     CDS             complement(3753..5804)
                     /locus_tag="PK502_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00880.1"
                     /translation="MRKLITPLILLTMLIFNQCKTTSNEVSQDVSPISDKTIGKVTTE
                     LVAKYGEAQKFRIERGVGQTALLWRSSDGSEADFEEFCGKQFISNPEELDQLFNKLSY
                     GYEMLFGHFILLSKELMRPLHLDWGPVAPVDELFGSYNAASHVTDDLYGNKIAFITTL
                     NFPYYNLKEKAEMGEKWSRKEWAYARMGDMFTSRVPAQLIQDFSTANTNADTYISSYN
                     IYMGSLVNDEGKKLFPNDMALITHWGLRDELKSQYSNLVDGLEHQRIIYQVMKRIIDQ
                     SIPLQVINKGDYQWNPNSNTLQRNGEDIAVEPEPNTRYQHVINQFNTLRAIDAYTPAM
                     PTYIQRKFEGEYELPVDDVEKLFIELVSSHEVREAGKLISQRLGRKLEPFDIWYDGFK
                     VRSGISAEKLDGLTTKKYPTVEAFDKALPEILTSLGFPKDKAQFIGDHVRVEAARGSG
                     HAWGAEMKKDDALLRTRVPATGMNYKGFNIAMHEFGHTVEQTISLHDVDYYTMHGVPN
                     TAFTEALAFVFQNRDLAVLGISETNPQKESLETLDNLWSCYEIMGVSLVDINLWKWLY
                     NHPGASAQEVKDATINIAKDIWNKYYADVFGSTDEPILAVYSHMIDNPLYLSAYPVGH
                     LIHFQLEKQLKGKSFASEIERIYSQGRLIPQLWLKDAVGETLSVKPILEASAEALGKL
                     K"
     gene            5982..6206
                     /locus_tag="PK502_08430"
     CDS             5982..6206
                     /locus_tag="PK502_08430"
                     /inference="COORDINATES: protein motif:HMM:NF039796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HPH00881.1"
                     /translation="MDCKKESNSVGCRCTYPCTRHGVCCDCLMYHRKKGQLPACYFPK
                     DVETGYNRSIANFTRVISERGTGFLMDDSE"
     gene            6392..8134
                     /locus_tag="PK502_08435"
     CDS             6392..8134
                     /locus_tag="PK502_08435"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HPH00882.1"
                     /translation="MSYSFESYRVEITDRQFTRSDGYTTPKTARVVLLNDRLSEIATL
                     NLAYPNLADVYEGIGRGETINLDCCLVQSFSATACKRYLGLDKSSLIPLLGLSAQNAF
                     FESGISIDFSLVDFRGDVNFNNSYMVAPLVDFHQSTHSNGNFSLSDCHIRTEHFNFTQ
                     CRFYGSNMSFKNTTFSPGEKDFQDCVFNSNDTLFTNTDFGNGNVSFINVKFGSSEINF
                     KVTRFGTGKVDFHYANFGRGDVSFERTEFGPGRVDFRTVDFGTGRVSFNRSVFGDGEV
                     NFEGAEVKAEKLTFKRASFGTGNLIFDFFQGDTSDLVFERTIFSTNLSFHGARLNNLL
                     MEDCQFNSMLNLHVEKCASISLPGCTIRDIAEFYTHGSKPMIDMLNISGVKLLGKLYI
                     DDEACNLKQLIYNQTDTTCTDKAEQFRILKENFNDLGRYKEEDRSYVEFKRCEAKARL
                     RRLKSKGFLHKIAGYVEYAAKKLIFDYMGLYATAPQRVIVSILVVYSLYSLSYALIML
                     MNIGIVEATGPSGAHLGLVAKGFYFSVVTFFTIGYGDFAPDGFARIIAGTEGFMGVFM
                     MSYFTVAFVRKILR"
     gene            complement(8156..>8542)
                     /locus_tag="PK502_08440"
     CDS             complement(8156..>8542)
                     /locus_tag="PK502_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPH00883.1"
                     /translation="ANQLSTMQNIILLCGHYKGVDHRVREHLVTREISIGDYVLSGGE
                     LAAAVIVDSVVRLVPGVLTDETSALTDSFQDGLLAPPVYTRPAEFNGWKVPEILISGN
                     FKEIEKWQLEQSIERTRRLRPNLLGE"
BASE COUNT         2400 a         1858 c         1940 g         2244 t
ORIGIN      
        1 gttcagcccg gcaagcctac aacgttcgga agcttggaca acaagctggt atttgggctg
       61 ccaggtaatc ctgtttcggc attcatacag ttcgagctac tggttaagcc cgccatacgt
      121 aagctaatgg gctgcaagca gctgaaccct acggccattc gtttgccttt agcagaggat
      181 tacaggcgaa acagggccga gcgaatggcg ctggttccgg ttaagataat agcagggtca
      241 gcagctttac ctgttgagtt tcacggctcg gcacacatat ttgcccttgg caatgccgat
      301 ggcattattg ctgtccctat tggtaccaca aaaattgata agggagaaat tgttgatgta
      361 cgaccgcttt aaccgccaaa tcaactacct gcggatatcg gtaaccgacc gttgtaacct
      421 acggtgcact tactgcatgc ccgaagaggg tattactcag cttgaccata gcgagatact
      481 ctcgtttgag gaaattgcat cctttacaag aattgcagcc acaaaagggt tcaacaaggt
      541 gagaattaca ggcggtgaac cccttgttcg aaaaggtgta gtggaactcg tgtccatgat
      601 ttccaacatt gagggcatca ccgacctgag catgaccacc aacggagtgc ttctgggcaa
      661 atatgctaag gagttaaaat atgctggttt acagagagtt aatgttagcc tcgatacggt
      721 caaccccacc aggtacaggc aaattaccag ggtaggtagc cttgacgatg tgcttaatgg
      781 gttggaatgt gccagagctg caggcctaac cccaataaaa ataaactgtg tagttgataa
      841 atcatcggca gaacccgacg ccgttgaggt aaaaaaattt gcccaagcaa atggatttga
      901 agtcaggttc attcctaaaa tggatttaaa cgccggaata ttcggcgagg tagagggggg
      961 aaacggcggt aactgcagca tctgcaatcg cctcaggtta acagccaacg gttttgtcaa
     1021 accgtgtctg tttagtaatc tgggctatag tgtacggggg cttggatatg agggagcctt
     1081 actcaaagca gtgaatgcca aacctgcccg tggaactacc aaccatgacg ggaaattcta
     1141 taacatagga ggttgacatg gaactttcac ataccgacag agatggcagg gccaacatgg
     1201 ttgacgttgg ctccaagccc aaccagctta gggaagcacg cgccaaagga tttatcaggc
     1261 ttcaacccga aaccctaaag cttatcacag agaatcaggt aaaaaagggt gatgtgctca
     1321 cccttgccga gattgccgga atacaggctg ccaaacaaac gccaacgctc atcccgcttt
     1381 gccacaccat actgctagct aaggttgatg taaaagcatc catcgttcaa aacgggattg
     1441 aaataagcag ctacattaag tgcatcggcc aaacaggtgt tgaaatggaa gccctcacag
     1501 ccgtttcagt tgccctgttg gctgtttacg acatgtgcaa agccgttgac aaggaaatga
     1561 caattgagtc cataaaactc accgaaaaaa taaaacatga cattaattcg taaagcaaaa
     1621 agttgtggat accagcgtaa aagtactatc ggttaatgtt tcggatgcaa agggcacagc
     1681 aaagcaccct gttgacttta ttgagctaaa cagcacaggg gttaaaggcg atgcccattc
     1741 cggaatgtgg caccggcaag tcagccttct cggtgttgag agcatcgaaa aatttaatgc
     1801 ggccaacaac ctgagaataa aatatggcga ctttgccgag aacatcacaa caaagggttt
     1861 ctgcctttac gagtgtattc ccggcgacag gcttgttggg tcgggcgttg agctggaggt
     1921 ttcccaaata ggaaagcttt gccatgccga tggttgcgcc atattcagcc aggttggtag
     1981 ctgcattatg ccaagggagg gaatatttgc cagggtctta aaaaccggaa ggctaaaagc
     2041 cggtgacgag ctggagctca taccacgaac ctacaaggtg aaagtggtta cgctaagcga
     2101 cagggccagc aggggtgact atgaggatag aagcggcccc gaggtggttc ggttggttaa
     2161 ccaattcttc aacgatatag gatggaggca ctccgttaat tccaccataa ttccggatga
     2221 tgccgctacg cttacctcaa ctgtggttga agccaagaac aatgggtacg acctcctgat
     2281 tactaccggg ggaacgggta taggaccaag ggatgttgcc cccgatgtgg ttaggccact
     2341 tcttgataag gaaattcccg gaataatgga gatgataaga atgaagtacg gagccgacaa
     2401 gcccaacgct ttaatcagca ggagtattgc cggggtgatg ggaagtatgt ttgtatatac
     2461 cctgcctgga agcgttaagg cagttggtga gtacatgcag gaaatcatca aaattcagaa
     2521 acacctgttc ctgatgctca tgggaataga cagccactag tgaagtcaac aatgatgtat
     2581 aaaccgtagg ttaaacgttc aaaagacaat cgtttctcaa gttgaaaaag aggaggaatt
     2641 gattaacgcc atcaaagagc taattgtgat atttgagcaa aagataaagg ctaggattgc
     2701 aggggttggg ggagaacata aaaagggagg ctctatttaa agaaaatgtt gttttccgat
     2761 agccatattg gaatcatgaa tagtaattat tgcgttcttt gtgtaatatc attgtgtgct
     2821 ttgtggtttc taatattatt taaccacaaa gttctcaaag aannnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnatcattgt gtgctttgtg gtttctaata ttatttaacc
     3001 acaaagttct caaagaaaag tttaataaat ttatcatata gaatgataat cggtattaca
     3061 acattatttt tgaatatggc caaaatgaaa aagattacag gttagaagac aaaacggtta
     3121 taaaggcggc agaaccataa atttatcaac aatccatatc gttgctaatc agttaattat
     3181 tacttatttt tataaaaaac attaatcata ccaataaaaa tttgataaga attaagcaat
     3241 aggttaattt tgacatgaag ataaaatcat acggaaatct aaagatggca agctatgcat
     3301 taccgttgat agcataaacg cctacgctat actagcctat ctacagcagg accgacgtca
     3361 cctaaacaag tttatagttg gcggtaattc taaaatgacg gtgcttcgtt gaaaggaatg
     3421 caaaacggac aacaatttta attacatctg tgagatgaat ttaggaatca atttgcaata
     3481 gtttcgaaaa aaatacaacc tgaatccgag aggatttttt acaacaagtc gcttgtcagc
     3541 cttctatcgg ttttctgtgc gtctgcacgc tgcccttgct tatggctttt tccagatttg
     3601 cagccacccg caacacccct gccatttgct agcacctcct ccctaaccgg cgtgtgcaag
     3661 aacttgcatc ttgccagtag tagtaaccaa aaagacctaa caggcattga aagcctgtta
     3721 ggtcgtataa aaaatttcag gtcttagccg gattacttca acttacctaa ggcttcagcg
     3781 gaggcctcca gaattggctt aaccgatagg gtttcaccaa ccgcatcctt cagccaaagc
     3841 tggggaataa gtcttccctg ggagtatata cgctcaatct cgctagcaaa cgacttgcct
     3901 tttagctgct tttcgagctg aaagtgaatt aaatgtccaa ctgggtacgc cgacaggtaa
     3961 agtgggttgt cgatcatgtg ggagtaaacc gccaaaatag gctcgtcggt gctgccaaac
     4021 acatcggcat agtacttgtt ccaaatatcc ttggcaatgt tgatggtggc atcctttacc
     4081 tcttgagcgg aggcaccggg atggttgtag agccacttcc aaaggtttat atccaccagc
     4141 gaaacaccca ttatttcgta gcacgaccaa aggttatcga gggtttcgag gctttccttc
     4201 tggggattgg tttccgatat tcccagaacg gccaggtcgc ggttctgaaa aacaaaggct
     4261 aaggcttcgg tgaaagcagt gttggggaca ccgtgcattg tgtagtagtc aacatcgtgg
     4321 agcgaaatgg tttgctccac agtgtgtcca aactcgtgca tggcaatgtt aaatcccttg
     4381 tagttcatgc ctgttgcagg gacacgggtt cgaagcaaag cgtcatcctt cttcatctcg
     4441 gcaccccatg catggcctga tcctcttgct gcttcgaccc gaacatggtc gccaataaac
     4501 tgcgccttat cttttggaaa gcctaacgag gtaagaattt caggtagggc cttgtcaaat
     4561 gcctcaacag taggatactt tttggttgta agtccatcca acttctcggc agaaatgccg
     4621 ctccgtacct taaacccatc gtaccagata tcaaagggtt cgagcttcct tcccagcctt
     4681 tggctgatga gcttgccagc ctcccgcacc tcatgcgacg aaacaagctc tataaacagc
     4741 ttctccacat cgtcaaccgg tagctcatac tctccttcaa acttccgctg tatgtaagtg
     4801 ggcattgcag gagtgtaggc atcaatagcc ctaagcgtgt taaactggtt aataacatgc
     4861 tggtaacggg tgtttggctc gggttcaacc gctatatcct caccattcct ttgtagggta
     4921 ttcgagttgg gattccactg gtagtcgcct ttatttatca cctgtaaagg aattgactgg
     4981 tcaataattc gcttcatcac ctggtaaatt atccgctggt gctcaagtcc atcgactaag
     5041 ttggagtact gcgatttgag ctcatcacgc aaaccccagt gagtaattag tgccatatca
     5101 ttgggaaata acttcttgcc ttcatcgttt accagacttc ccatgtagat attgtagctg
     5161 ctgatgtagg tatcggcatt ggtattggcg gtggaaaagt cctgtatcag ctgggcggga
     5221 accctacttg taaacatgtc gcccattcgt gcgtaagccc attccttccg cgaccacttc
     5281 tcgcccattt cagctttttc cttcaggttg tagtatggaa agttaagggt ggtaataaag
     5341 gcaattttgt tcccgtaaag atcatcggta acatgcgagg cagcattata ggaaccaaac
     5401 aactcatcaa caggggcaac aggcccccaa tcaaggtgaa gcggacgcat cagctccttg
     5461 ctaagtagta tgaaatgccc aaacagcatc tcgtatccat atgaaagctt gttgaatagc
     5521 tggtcaagct cctcggggtt ggaaatgaac tgcttgccgc aaaattcctc aaaatcggcc
     5581 tcacttccgt cggagctacg ccaaagtaat gcggtttggc caacgcccct ctcaattcta
     5641 aacttttggg cttcgccata ctttgctacc agctcggtgg taactttgcc tatggtttta
     5701 tcactgatag gcgaaacgtc ctggctaacc tcattacttg tggttttaca ctggttgaat
     5761 ataagcattg tcaataaaat taatggggta atcaactttc tcatggcata aggtttttag
     5821 gttgaacatg aattacacga tgcaaaaagc atttcattat ttcgacttca aagtaaagat
     5881 taagtttaaa gctaacctaa tccacaagtc cttcatcata aaaatactga tgtttttgaa
     5941 tgatgataat cctttcagcc tatatttgta gttaaaaaac gatggattgc aagaaagaat
     6001 ccaactcggt aggttgccgc tgcacatacc cctgtaccag gcatggtgta tgctgtgatt
     6061 gcctgatgta ccaccggaaa aagggtcaac ttccagcctg ttactttcca aaagatgtgg
     6121 aaactggcta caatcgctcc attgctaact ttacaagggt aataagcgag cgcggaacag
     6181 gatttctgat ggatgattca gagtaaaacc tatgcaagat catctcaaca tagctattca
     6241 aaacctacac gctgaacatt gatgtataga taagaaaggg caaaggcata acagggccaa
     6301 ggagtaatat cattttcctt ttaaaaaata ttctaaaacc tgtcaaaatt tggatatttt
     6361 tcatattttt acatgaacta caacatcctg aatgtcctat agttttgaat cgtatcgggt
     6421 agaaattacc gaccgacagt ttacccgctc cgacggttac accaccccta aaactgcgcg
     6481 tgtagtgctc cttaacgaca ggttatcgga gatagccacg cttaacctgg catatcctaa
     6541 tttggcggat gtttacgaag gtattggcag gggtgaaacc atcaaccttg actgctgctt
     6601 ggttcaaagt ttttcagcta ctgcatgtaa gcgataccta ggcctggata aaagttccct
     6661 gatacccctt ttgggattgt cggcccaaaa tgcatttttt gaatcaggca tcagcattga
     6721 cttttcgttg gttgattttc gaggtgatgt gaactttaac aactcctata tggttgcccc
     6781 tctggttgat tttcaccagt caacccattc aaatggcaac tttagcctat ccgattgcca
     6841 catccgaacc gagcatttca actttaccca atgcaggttt tatggcagca atatgtcgtt
     6901 taagaatacc acctttagcc cgggggagaa ggactttcag gattgcgttt ttaacagcaa
     6961 cgataccctt ttcaccaata ccgactttgg caatggaaat gtatccttta tcaacgttaa
     7021 atttggcagc agcgaaataa acttcaaggt gacccggttt ggaaccggta aggttgattt
     7081 tcactatgcc aacttcggaa ggggtgatgt ttcgtttgaa cgtaccgaat ttggacccgg
     7141 aagggttgat ttccgcaccg tagattttgg caccggaagg gtatcgttca accggtctgt
     7201 atttggcgat ggtgaggtga actttgaggg tgccgaggtt aaagctgaaa agctaacctt
     7261 caagcgggca tcctttggca ccggtaacct gatatttgat tttttccagg gtgatacttc
     7321 cgatctggta tttgaacgaa caattttcag cactaacttg agttttcacg gcgcaaggtt
     7381 aaacaaccta ttaatggagg attgccagtt taactccatg ctcaaccttc atgtggaaaa
     7441 atgtgcttca atcagccttc ctggttgtac aatcagagac attgcagagt tctacaccca
     7501 cggcagcaaa ccaatgattg acatgctgaa catttccggg gtcaagctgc tgggaaaact
     7561 ctacattgac gacgaggcct gcaaccttaa acagctgata tacaaccaaa ccgataccac
     7621 ctgtaccgac aaagctgaac agtttagaat tcttaaagaa aactttaacg atcttggcag
     7681 gtataaggag gaagacaggt cgtacgttga gtttaaacga tgcgaagcta aggcaaggct
     7741 ccgaaggctc aaaagcaagg gctttcttca taaaattgct ggttatgttg agtatgccgc
     7801 aaaaaagtta atattcgatt acatggggct atatgccact gctcctcagc gggttatcgt
     7861 aagcattctg gttgtatatt cgctttacag cctatcctat gcgctgataa tgctcatgaa
     7921 cataggaatt gttgaggcaa caggtccttc gggtgcccac cttgggttgg ttgcaaaagg
     7981 tttctacttc agcgttgtta ctttcttcac cattggctat ggcgattttg ctcccgatgg
     8041 atttgcacga attatcgccg gcaccgaagg gtttatgggg gtttttatga tgtcgtactt
     8101 tacggtggct tttgttagga aaattcttag gtagctaaaa cgactaaaac aatattcact
     8161 caccaagtaa gtttggacga agcctacggg ttcgctcgat ggattgttcc agctgccact
     8221 tttcaatctc cttaaagttt ccggaaataa gaatttcggg cactttccaa ccgttaaatt
     8281 cggcaggccg ggtgtaaacc ggaggtgcaa gcagcccatc ctgaaacgaa tcggttaaag
     8341 cagaggtttc gtcggttagc acacctggca caagcctcac cacgctatct acaataaccg
     8401 cagcagccag ctcacctccc gaaagcacgt agtcgccaat tgaaatttcc cgcgtaacca
     8461 aatgctccct aacgcggtgg tcaactccct tatagtgccc acaaagtaga attatgttct
     8521 gcatggtgct tagctggttg gc
//
LOCUS       DAOPHX010000111         8472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_10564_length_8472_cov_5.664489, whole genome shotgun sequence.
ACCESSION   DAOPHX010000111 DAOPHX010000000
VERSION     DAOPHX010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10564_length_8472_cov_5.664489"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PK502_08445"
     CDS             <1..894
                     /locus_tag="PK502_08445"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPH00884.1"
                     /translation="FEAIKSKVPQSEVSYIPGVSYPAYGRYFEEQYDKFEESAAAARK
                     ADVIILCLGENSYAEKPGDLNDLYLGSKQLNLAEELAKTGKPIILVLNEGRPRVISPI
                     EPMMAAVVQTYLPGSYGADALADILFGDINPSGRLPYTYPSYPNSTVVYYHKPAEEQK
                     PNIGAYSYESDYNPQYPFGYGLSYTNFEYSQLTVNRSELKQNDTLTISVNVRNTGDRK
                     GKDVVQLYTSDLVATITPDVKRLRKFAKIELAPGEAKTLTFKLVTSELAFVDSNDRLV
                     VEPGEFIVSVGNLSTKFHIVN"
     gene            920..3895
                     /locus_tag="PK502_08450"
     CDS             920..3895
                     /locus_tag="PK502_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPH00885.1"
                     /translation="MKYFLKIFRRNARILLLGLLCVCWIPSMAQTITVSGRVTDKEGA
                     PMAGVNIVVKGTTNGTNTDVDGKYSISTPANAILQFSFIGMKSVEEAVGGRSIIDVVL
                     ESESMDISEVVVIGYGTSRKKDITTAVSVVSTEDFDKRPVTNVAQAIQGKAAGVQVVL
                     PSGKPGGDAFIRVRGATSVLAGNDPLFVIDGVPTTSMQALNPQDIETVQVLKDASSAA
                     IYGARAANGVVIITTKHAKAGVASVSVNSYMGFSNLANSLDVLNTEQYRELMDEVKGP
                     GTVPTSQTNYTNWIDETFSTGQIQNYQVSVSNGTEKMKYYFSAGFSDEKGIVKPAENK
                     KYTIRTNINSDLFKWLKFGTNISFIHNWYNDVSDNANSGRAGTILSAINTPPFLHIWD
                     PNNPEQYEVNPFQNSWENPLAYASRINQSKTERVLANANTEITFLKDFKLKSNFGIDG
                     YIYQRDLYIDPVKTVYGRTENGIGEADKSNSLVWLIENVLSYEKTFGNHNLSVQAGQS
                     AQSSRWDQSYISAKDFPKNSAVTTINAANQITYAASSASEWAIASFFGRVSYNYMSKY
                     LFTANFRYDGSSKLSSDNRWDFFPSFSAAWRLSSEPFLKNVVFINDLKIRGGWGRNGN
                     QEGIGDYAWQALYGFSRQSPTSPLSGPGIYQMNLENPSLKWEVTYQSNVGLDLTVLDS
                     HLSFTGDVYWKNTDGLLVNVPLPSGSAAVDYITRNDGKMKNWGVELSLDAKDFRHELK
                     WSTNLFWSMNRNEITELQLSQIYNFAQLESNNEYVIRMSPGYSLGTFYGYVAKGVDPE
                     TGDMIYEDRNNNGVIDPDDRTVIGSAEPDFIYGLNQTLTYKGIGLTLFFQGSQGNDIF
                     NASRIDTEGMFDVKNQTTEVLRRWKRPGQITDIPRAVSNGSTFNVHNSTRFVEDGSYF
                     RLKALTLSYSLPSKWLSRVSIKNFTLYATATNLFTITGYNGFDPEVNYGGNNSVIQGV
                     DYGTYPQSRSYIFGLSFDF"
     gene            3908..5389
                     /locus_tag="PK502_08455"
     CDS             3908..5389
                     /locus_tag="PK502_08455"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPH00886.1"
                     /translation="MKKLNGYLLAGVALLTFTVVSCEDFLDKDPISNYSNSTLGVDNN
                     TDTLKYTTAEQAEALLATCYSNFRGELFQLDYFVNGDMQSDDAYAGASGTEFSQFDNF
                     SIDATNKNVERDWKYLYKMVTDCNTVIVNVPKITDQALTDARKKEIVAEASFIRAWAY
                     FDLVRLYGDVPLVLDVVTTITADNVAEVYPLLYPKRDSRELVYDQIITDLNTALDGVP
                     VTAPNKGYVTKGAVNALLARVYATRQPIDYTKVNDYCDAVINGGYSLLSNYDYLWDFQ
                     HENSSEAIFEIQYDGNWNTGTGNWAVSMFLGTGWKKFNTPSNDLVAAFDAENDVIRKN
                     SSIKFEDVTGSWTDANWPTTTYPFIYKIRDEASTSNIILMRLADFILLKAEALNELGL
                     PGADTLVNQIRGRVNLPSVSPTTKEQMRLIIEKERRLELAFEGIRWFDLLRTGRAITV
                     MENVRGGNGELLYAGKLQPYKLLWPIPQEQRDLNSMLTQNPGY"
     gene            5410..6762
                     /locus_tag="PK502_08460"
     CDS             5410..6762
                     /locus_tag="PK502_08460"
                     /inference="COORDINATES: protein motif:HMM:NF027723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5016 domain-containing protein"
                     /protein_id="HPH00887.1"
                     /translation="MKILKYTCIVGLALVAFTACEDNETKTTSQPTISNVSISPTAGL
                     TYGDSIAVSASVSDEVTPLSTLDIQVIVADVEIYKKSIRTKGNSANVSAKFKLPFAAN
                     AEDNGEVTVKLQSINIDGFSTSQTETLHAARPILGDILYLVTSSDTIELAKSPENPFL
                     YLSPTGSYPNEVVGKIATSENLEAAEWVWVSSGSAIVTGSYTDSDIRLSDPSYQVEQL
                     SFNVMTFELKFIGELLPSVKFNTTTLLMGEDGRFHGEVEFTKGDVVTVEGISNLAAAY
                     NPDFFDYKDGVLTFKAPTGKYQVVYWAGTINYMWLRQDNAVYPDALWLTGAGIGMPTF
                     INDVTNIWWGWDDWGVTTRQSYFFCPKIGENKYQVTVYLMNTEGSWWLQTEFKLFPTK
                     SWDNEFSSMDWGYISSNLSPNTNGNFVHTPELTEGLYRITIDMSGGKGNYFLIAQKIN
                     "
     gene            6932..8398
                     /locus_tag="PK502_08465"
     CDS             6932..8398
                     /locus_tag="PK502_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HPH00888.1"
                     /translation="MKNIHNSLFSFASGMLSACLIFFAILLPGCTDDSDSVDNPPDDT
                     TQLGDVRVWVTTCNQAYLFKEQSVSFSDDVAPFTIRVDTLNRYQQVEGFGAALTGSSA
                     YNLRRMTDSQRTSLLTELFDPVKGIGLSYLRLSIGSSDFSLSLYTYCDYPSIDSFALH
                     PTDLRDLIPVLKEILAINPAIKIMATPWTPPIWMKTSAAWSGGSLIPDFYDEYAEYFV
                     RYIRAMAGEGITIDAISVQNEPMWEFGTPGMKMTWQEQLTFIRDFLGPKFAAEDISTK
                     ILIWDHNWDMPDYPMSILSDPAAYMYIAGSAFHGYGGEYSAMLSVHNQFPQKGLYFTE
                     ISGGGWATDFDDNLKWTIENVFFGTLSCFSKNVLLWNLVLDDNWGPFIQGGCSNCRGV
                     ITLNSDGSLTRFNEYYALAHFAKAIRPNAYMLGTTVVGDMSQLADFKYVAAQNPDGSK
                     VVVAMNFSSVGKKLAVRCGSNKFTFTVQPESLVTFMWR"
BASE COUNT         2221 a         2009 c         1986 g         2256 t
ORIGIN      
        1 tttgaggcca ttaaaagtaa agtgccccaa tcggaggtca gctatattcc cggggtaagc
       61 tacccggctt acggacgata ctttgaggaa cagtacgata agttcgaaga gtctgctgct
      121 gccgctcgta aggccgatgt tataattctt tgccttggcg aaaacagcta tgctgaaaaa
      181 cccggcgacc ttaacgacct gtacttgggg agcaagcagc ttaatttggc agaagagttg
      241 gcaaaaaccg gtaagcccat catacttgtt ttaaacgaag gacgacccag ggttattagc
      301 cctattgaac ccatgatggc agcggtagtt caaacctacc ttccgggtag ctatggtgcc
      361 gacgcactgg ccgacatcct ttttggcgac attaaccctt ccggcaggtt accctacact
      421 taccccagct acccaaactc taccgttgtt tactaccata aaccagccga ggagcaaaag
      481 ccaaatattg gagcctacag ctatgaatcg gactataacc cgcagtaccc atttggctat
      541 ggacttagct acactaattt tgaatacagc cagctgacgg ttaaccgaag tgagcttaag
      601 caaaatgata ccctaaccat ttccgtaaat gttcggaata ctggagatag aaagggaaag
      661 gacgtggttc agctgtacac ttccgactta gttgccacca ttacccccga tgtgaaacgc
      721 cttaggaagt ttgccaaaat tgaacttgcc ccaggtgaag ctaaaacttt aacttttaag
      781 ctcgttacca gtgagcttgc ttttgttgac tccaatgata ggcttgtagt tgaacctgga
      841 gagtttatag tttcggttgg aaatctaagc accaaattcc atatagtaaa ctaatgtttc
      901 aacccttaaa tctaaagcta tgaagtattt cctgaaaatt tttagaagaa atgcacgcat
      961 actcctactg ggattgctat gcgtgtgttg gattccttca atggcgcaaa ctattacggt
     1021 ttccggaagg gttaccgata aggagggagc gccaatggca ggtgtaaata ttgttgtaaa
     1081 gggaaccacg aatgggacaa ataccgacgt ggacggaaag tatagcataa gcacaccggc
     1141 gaatgctatc ctacagttct cctttattgg tatgaagagc gttgaggagg ctgttggcgg
     1201 tcgttccatt attgatgtag ttctcgaaag cgaaagcatg gacatttctg aggtggtggt
     1261 tataggatat ggcaccagcc ggaaaaagga cattaccacc gctgtttcgg tggtttcgac
     1321 tgaagatttt gacaagcgac ccgtaaccaa cgtggcacag gcaatacagg gaaaggccgc
     1381 aggagttcag gttgtgcttc cttcgggtaa acccggaggg gatgcattta tcagggttag
     1441 aggtgcaaca tcggtgctgg ccggtaacga ccccctgttt gtaattgatg gagttcctac
     1501 taccagcatg caagcactta acccgcagga tattgaaacg gttcaggtgc tgaaggatgc
     1561 ctcttctgcc gcaatttatg gcgcacgtgc tgcaaatggt gtggtaatta ttaccaccaa
     1621 acatgcaaag gccggtgttg cttcagtttc ggttaatagc tacatgggct tctcaaacct
     1681 ggctaactca ctggatgtgc tcaacaccga gcagtaccgg gagttaatgg acgaagtaaa
     1741 gggaccagga actgttccga cctcccagac taactacacc aattggattg atgaaacctt
     1801 cagcaccgga caaattcaaa actaccaggt ttcggtttcc aacggtaccg agaagatgaa
     1861 gtactacttt tcggctggtt tttccgatga aaagggtatt gttaagcccg ctgaaaataa
     1921 aaagtacacc attagaacca acatcaacag cgacctgttc aagtggctta aatttggaac
     1981 caacatcagc tttattcata actggtacaa cgatgtaagc gataatgcaa actccggaag
     2041 ggcaggtacc atccttagcg ccattaacac cccacccttc cttcatatct gggatccgaa
     2101 taaccccgaa cagtacgagg ttaacccatt ccagaattca tgggaaaacc ctctggctta
     2161 tgcttcgcgt atcaaccagt cgaaaactga gcgggtgctg gccaacgcaa ataccgagat
     2221 taccttctta aaggatttta agctgaaatc aaatttcggg attgatgggt atatttacca
     2281 gcgcgatctc tacatcgacc ctgttaaaac cgtttatgga cgtacggaaa acggaattgg
     2341 cgaggccgat aagtcaaact ccttggtttg gttgatagag aacgtgctat cctacgaaaa
     2401 gacctttggc aaccacaacc tctctgttca ggcaggtcag tcggcccaat cgtcacgttg
     2461 ggaccaatcc tacatatccg ctaaagactt tccaaagaat tctgctgtaa ctaccattaa
     2521 tgctgctaac cagattacct atgctgcatc aagcgcttcg gaatgggcaa ttgcatcatt
     2581 ttttggacgt gtatcctata actacatgag taaatacctc tttaccgcta acttccgcta
     2641 cgacggttct tccaagctat cctccgataa ccggtgggac ttctttcctt cattctcggc
     2701 tgcatggagg ttatcgtccg agccattttt aaagaatgtc gtctttatta acgatttgaa
     2761 aattcgtggt ggttggggta ggaatggaaa ccaggaggga attggcgatt acgcatggca
     2821 ggctctttat ggtttttccc gtcaatcccc caccagcccg ctttcgggac ccggtattta
     2881 ccagatgaac ctggagaacc cttcgcttaa atgggaggtt acttaccagt ccaacgttgg
     2941 gctcgactta accgtgctgg actcgcattt gagcttcacc ggcgatgtgt actggaagaa
     3001 taccgatggc ctgctggtga atgttccact cccttcgggc tctgctgcag ttgattacat
     3061 tacccgtaac gatgggaaaa tgaaaaactg gggcgtggag ctgtcgctgg atgccaagga
     3121 cttcaggcat gagctgaaat ggagcacaaa tcttttttgg tcgatgaacc gcaacgaaat
     3181 caccgaactt cagctttcgc agatctacaa ctttgcccag ctcgaaagca acaacgagta
     3241 cgttatccgg atgagcccgg gttactccct tggaacattt tacggatatg tggcaaaggg
     3301 agttgacccc gaaaccggcg atatgattta tgaggacagg aataacaatg gcgttattga
     3361 ccctgacgat cgaacggtta ttggttcagc tgaacccgac tttatctacg gcttaaatca
     3421 aaccttaacc tacaagggaa taggcttaac cctgtttttc caaggttcac aggggaatga
     3481 tattttcaac gcctcccgga ttgacaccga gggcatgttt gacgtgaaaa atcaaacaac
     3541 cgaggtgctg cgcaggtgga agcggccggg tcaaatcacc gatatacccc gcgctgtgag
     3601 caacggtagc actttcaatg tgcacaactc tacccgcttt gttgaggatg gatcatactt
     3661 taggcttaag gctcttaccc tgtcctactc cttaccgtca aaatggctca gcagggttag
     3721 cattaaaaac ttcacccttt atgcaactgc taccaacctg tttaccatta caggttacaa
     3781 cggattcgat cccgaggtta actacggtgg aaacaacagc gtaattcagg gtgttgacta
     3841 cggcacctat ccgcaatcga gatcatacat atttggttta tcgttcgact tttaaactcc
     3901 gagagatatg aaaaaactta atggatacct gcttgccggc gttgctcttt taacattcac
     3961 ggtggtttcg tgtgaggact ttctcgacaa ggaccccatt tcgaactact ccaacagcac
     4021 gctgggcgtt gataataata ctgacactct aaagtacact actgccgaac aggcagaagc
     4081 gctgctggcc acctgctact ccaacttcag gggcgagctg tttcaactcg actactttgt
     4141 caacggcgat atgcagtccg acgatgccta cgccggtgcc agcggaaccg agttttccca
     4201 gtttgacaac ttcagcatcg acgctaccaa caagaacgtg gagcgcgatt ggaagtacct
     4261 ttacaagatg gtaaccgatt gcaatacggt aattgtaaac gttcctaaaa ttaccgacca
     4321 ggcattaacc gatgcccgta aaaaggagat tgttgccgag gcatcgttta ttcgtgcatg
     4381 ggcatatttc gaccttgttc gcctttacgg cgatgttccc ctggtgctcg atgtggttac
     4441 caccatcaca gccgataatg ttgccgaggt ttaccccctc ctttacccta aaagggactc
     4501 cagggagttg gtttacgatc agattattac cgatttgaat acagcgcttg atggtgtgcc
     4561 ggttactgct cccaacaagg gctatgtaac caaaggtgct gttaatgcct tgctggcaag
     4621 ggtttatgct acccgccagc caattgacta cacaaaggtg aacgactact gcgatgccgt
     4681 gattaacggt ggctacagcc tgttaagcaa ctacgactac ctgtgggatt ttcagcacga
     4741 aaactccagt gaagcaattt ttgaaatcca gtacgacggt aactggaata ccggtacagg
     4801 caactgggct gttagcatgt tccttggcac gggatggaag aaattcaaca ccccatccaa
     4861 cgacctggtg gccgcttttg atgcagaaaa tgatgttatc cgcaaaaact cttcaattaa
     4921 gtttgaggat gttacaggta gctggactga cgccaactgg cccacaacca cttacccatt
     4981 tatttataaa attagagacg aggcaagtac ctccaacata attctcatgc ggctggccga
     5041 ttttatcctg ctaaaagctg aagcccttaa tgaacttggt ctccctggcg ccgatacgct
     5101 ggttaaccag attaggggca gggttaacct tccttcggtt tcgcctacca caaaggagca
     5161 aatgaggctg attattgaga aggaacgtcg cttggagttg gcttttgagg ggattcgctg
     5221 gttcgattta cttcgaacag gacgggccat tacggtgatg gaaaatgttc gtgggggcaa
     5281 tggcgagttg ctgtatgccg ggaaactcca accatataaa ctcctttggc ctattcctca
     5341 agaacagcgc gatttgaata gcatgttaac gcaaaatcct ggttactaaa atcatataaa
     5401 tttgatacca tgaagatctt aaaatatacg tgcattgtag gtttagcgct ggttgcattt
     5461 acagcctgcg aggacaatga aacaaaaact acctctcaac ctacaataag taatgtttcc
     5521 atctccccaa ccgccgggtt aacctatggt gattccattg ccgtttctgc ttcggtttcc
     5581 gatgaggtaa cgccactatc aacccttgac attcaggtga ttgtggctga tgtggaaatc
     5641 tataaaaagt caattagaac taagggtaac agtgctaatg tttccgctaa gttcaaactg
     5701 ccctttgctg ccaatgccga ggataatggt gaggttaccg tgaagctcca gtctatcaac
     5761 attgatggct tttctacttc tcaaaccgaa acgctccatg cagcccgacc tatacttggc
     5821 gatatccttt acctggtaac ttcgagcgat accattgagc tggcaaaaag ccctgaaaac
     5881 ccgttcctct accttagccc tacggggtcg taccccaacg aggttgttgg taaaattgca
     5941 acctccgaaa accttgaagc tgccgagtgg gtttgggttt cctcgggtag tgcaattgta
     6001 accggcagct acaccgatag cgatatcagg cttagcgatc catcctacca ggttgagcag
     6061 ctttccttca acgtcatgac ttttgagctg aagtttattg gcgagctgct gcccagcgtt
     6121 aagtttaaca ccaccacgct gcttatgggt gaggatggac gtttccatgg cgaggtagaa
     6181 tttaccaagg gtgatgtggt taccgtagag ggaatttcta acctggcagc tgcctacaat
     6241 cctgactttt tcgactacaa ggacggcgtt cttactttta aagcccccac cggaaagtac
     6301 caggttgttt actgggctgg aaccatcaac tacatgtggc tgcggcagga taatgccgtt
     6361 taccccgatg ccctttggct taccggtgcc ggaattggta tgcccacctt tattaacgat
     6421 gtaacgaata tctggtgggg atgggatgat tggggcgtaa ccacacgcca aagctacttc
     6481 ttttgtccaa aaattggcga gaataagtac caagttactg tttacttgat gaataccgag
     6541 gggtcgtggt ggttgcaaac cgagtttaag ctctttccta ctaagagttg ggataatgag
     6601 ttctcctcaa tggattgggg gtacatctcc agcaacttaa gtcccaatac caatgggaat
     6661 tttgttcaca cccccgagct aaccgaaggg ctgtacagaa taaccattga catgagcggc
     6721 ggaaagggga actatttcct gattgcccag aagattaact agggttggtt ttaggtttgc
     6781 gtgcttatgt tgaagaatta acctgtttgg attggcatag tcctacaggt taattttttt
     6841 ccctccgttt gtgttaaaat cctttttaaa atagattggg ttcagctaac ttttgcactt
     6901 caactttaca atgtccatct caattcaccc gatgaaaaat atacataaca gcctattttc
     6961 ttttgcttcc ggaatgctgt cggcatgttt gatttttttt gccatccttc tgcctggctg
     7021 cacggatgat tctgacagtg tagataatcc gcccgacgac accacccaat taggtgatgt
     7081 tcgggtttgg gtaaccacct gtaaccaggc ttacctcttc aaagagcagt cggtttcctt
     7141 ttcagacgac gtggcgccct tcaccatacg ggttgatacc cttaaccgat accagcaggt
     7201 tgaaggtttt ggtgctgcac ttaccggttc gtcagcctat aaccttaggc gtatgacaga
     7261 cagccaacgg acaagcctgc ttacagagct tttcgatccg gtaaagggca ttggacttag
     7321 ctacctgcgc cttagcattg gctcttccga tttttccctc agcctttaca cctactgcga
     7381 ctacccatct attgattcgt ttgccctgca tcctactgac cttcgcgatt tgattcctgt
     7441 tcttaaggaa attcttgcca ttaatcctgc tattaagata atggctacac catggacacc
     7501 tcctatttgg atgaaaacaa gtgctgcatg gagtggtggc agtcttattc ccgacttcta
     7561 cgacgagtac gccgagtatt ttgtccgcta cattagggct atggctgggg agggtattac
     7621 tattgatgcc attagcgttc agaacgagcc catgtgggag tttggtactc ccggcatgaa
     7681 gatgacctgg caggagcagc tcacctttat tagagatttc ttaggaccta agtttgctgc
     7741 agaggatatt tcgaccaaaa ttcttatttg ggaccataac tgggatatgc ccgactaccc
     7801 catgagcatt ctttccgatc ctgctgccta catgtacatt gccggttcag cattccacgg
     7861 gtatgggggc gagtactccg ccatgctaag cgttcacaac cagtttcccc aaaaggggct
     7921 atactttacc gaaatctcgg gcgggggatg ggcaaccgac tttgacgata acctgaaatg
     7981 gaccattgag aatgtattct ttggaaccct ttcgtgtttt tcgaagaatg tgcttctatg
     8041 gaatctggtg ctcgacgaca attggggacc tttcattcag ggcgggtgct ccaactgccg
     8101 tggggttatc acccttaact ccgatggaag tcttacccgg ttcaacgagt actacgcttt
     8161 ggcccatttt gcaaaggcaa taagaccaaa tgcctacatg ctgggtacaa cagttgttgg
     8221 tgatatgtcc cagctggcag acttcaagta tgttgctgct caaaatcccg atggttcaaa
     8281 ggttgtagtt gccatgaatt tttccagcgt cgggaaaaaa cttgctgtaa ggtgtggtag
     8341 caataagttc accttcaccg tccagcccga atcgctggtt acattcatgt ggaggtaagg
     8401 taaaagataa aggataaaga acttgtccgc ctactggcgg aacttgtccg gctcctggcg
     8461 gattcaccct tc
//
LOCUS       DAOPHX010000112         8442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_10615_length_8442_cov_6.688923, whole genome shotgun sequence.
ACCESSION   DAOPHX010000112 DAOPHX010000000
VERSION     DAOPHX010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10615_length_8442_cov_6.688923"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="PK502_08470"
     CDS             complement(<1..151)
                     /locus_tag="PK502_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00889.1"
                     /translation="MNIFKKTIELGDGRTITLETGKLARQAHGSVVLTMGKTMLLATV
                     VSAQEA"
     gene            complement(487..759)
                     /gene="rpsO"
                     /locus_tag="PK502_08475"
     CDS             complement(487..759)
                     /gene="rpsO"
                     /locus_tag="PK502_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPH00890.1"
                     /translation="MNYLTVEKKKEIFREYGKSDTNTGSSESQIALFSYRINHLTEHL
                     KTHRKDYATQRSLLKMVGQRRRLLDYLKEKDIDRYREVVKALNLRK"
     gene            complement(1329..1916)
                     /locus_tag="PK502_08480"
     CDS             complement(1329..1916)
                     /locus_tag="PK502_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPH00891.1"
                     /translation="MNANNRSDQELLSEFKEGSNAAMEVLIKRHERRVYTYILLMVHN
                     SHLADDLMQETFVKAIKSLRGGKYSESNRFQSWLLRIAHNLVIDYFRQKSHYREVSNE
                     EYGVDLLNTPRFSEMNAEQRMVYERVIAEVRMMVDGLPPSQREVVMLRYYADLSFKEI
                     ADITNVSINTALGRMRYALINLKKVVKEKNLCLEY"
     gene            2227..5028
                     /gene="uvrA"
                     /locus_tag="PK502_08485"
     CDS             2227..5028
                     /gene="uvrA"
                     /locus_tag="PK502_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPH00892.1"
                     /translation="MTRSVENVISVKGARVHNLKNISLDIPRNALVVITGVSGSGKSS
                     LAFDTIYAEGQRRYVESLSAYARQFLGRMAKPEVDLISGIPPAIAIEQKVNTRNPRST
                     VGTSTEIYDYLKLLFARIGRTYSPVSGKEVKKHSVSDVVNFISSFNEGTKMMLLAPLT
                     PNGDMKQKLEALAHEGFTRVEVGNQIVRIDEQKFREVVDLSGSELRLVIDRFAAAADD
                     EFLSRLADSVQTAFAEGKGFCLLKVFDDNGSYSLEQFSSLFEMDGIKFEEPTEHLFSF
                     NSPLGACPTCEGYGKIVGIDENLVIPNRSLSVYDDAIACWKGESMSWYKDRVVENASR
                     YGIPIHKPYFQLSEEQKSLLWEGTPHFTGINDFFRELEGKKYKIQYRVLLSRFMGKTT
                     CPTCRGGRLRREATFVKVNGYTIQELVEMPVADLAEVFNNLELTEHEVGVAKRILIEL
                     KNRLNYLVNVGLGYLTLNRLSSSLSGGESQRINLATSLGSSLVGSLYILDEPSIGLHP
                     RDTHLLIDVLKKLRNMGNTVVVVEHDEEIIRASDYIVDIGPYAGVNGGEVVFEGPIEN
                     LEKATQSLTANFLLSREQILVPRVRRKWNSYIEISGARENNLKGIDVKFPLSCLVVVT
                     GVSGSGKSSLVDDILYPALLKQLTGAGPRAGQFDSLGGDWKLISGVELVDQNPIGRSS
                     RSNPVTYIKAWDEIRKLLSEQPYAKRNGYKPAHFSFNVEGGRCEECQGEGVIHVEMQF
                     MADLTLVCESCKGKRFKDDILEVRYNGANVYDILNMTVDQAIEFFSKGKTLTEQKVVD
                     RLKPLQDVGLGYIQLGQSSSTLSGGESQRVKLASFLQKDASIRPLLFIFDEPTTGLHF
                     NDIRQLLNAFDALLAKGHSLVIVEHNLEVIKSADWVVDLGPEGGQEGGLLVFAGTPED
                     LAANEASHTGVFLKKKLNG"
     gene            complement(5073..5513)
                     /locus_tag="PK502_08490"
     CDS             complement(5073..5513)
                     /locus_tag="PK502_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00893.1"
                     /translation="MKSFTTTILILGLLLLQPHSTQAQCKNFAKKICKLELAPYVHDG
                     NYNAAILTEGEDAELFKTFYAGQEYRISICGSESLAKIEFLVMDVDRRVLYSNAKNNY
                     ATSWNFKVEATQQLIILVRVPSSGNSGSELASGCVAIMFGLKAE"
     gene            complement(5530..6447)
                     /locus_tag="PK502_08495"
     CDS             complement(5530..6447)
                     /locus_tag="PK502_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00894.1"
                     /translation="MATGFVKRIVKLLGLLLIVGGVACKSEKPSDKSLKVTEDELAAD
                     SVISQSIQSVKQIFYSLPSPLETALILKRSGAVYNEDLLNPVENVAHYNTSKSMALNL
                     GIYSTDLSYASLFDQTQATINLMNASKKMADGLGILNAIDNSVIQRLEENINNRDVIM
                     ETISETFLNTNSILEEDDRVAIGSIILVGGWLEGLYLATSLVSDEKKMDNELVDRIAD
                     QKFSLETVLSLLKQNSDNADVMEIYSDMQKLSEIFYDIQISASRVEVVPASNNGATIL
                     RSKNVITVNPDVFNKLKTKVKELRTKYTA"
     gene            complement(6667..7182)
                     /locus_tag="PK502_08500"
     CDS             complement(6667..7182)
                     /locus_tag="PK502_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00895.1"
                     /translation="MLAEILKIVLPALMVFLAGYLAISAMIKSETNRRKSELMFNNIK
                     ITTPVRLQAYERLVLFLERISPESLLPRTTKKGMLASDLHTALLGTIRAEWEHNLSQQ
                     LYVSKEAWEMVKNAKENIIKLINVCADRINPNETSMALSQKIIETYVELDTNPTTLAI
                     DFLKKEVAEIF"
     gene            7271..>8442
                     /locus_tag="PK502_08505"
     CDS             7271..>8442
                     /locus_tag="PK502_08505"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPH00896.1"
                     /translation="MEKVSSRIAALEESQTIAMSQKSRELAAQGIDVVNLSLGEPDFF
                     TPDFIKEAAKRAIDENFSFYPPVSGYKDLLEAICGKLKRENDLTFTPDQVVVSAGAKH
                     SLSNVLMCVVEKDDEVVVPAPYWVSYVELIKLAEGRSVIVPTTIENDFKITPQQLEAA
                     ITPKTRAFLFCSPSNPTGSVYSKAELEAIARVIEQHPNIIVISDEIYEHINFVGKHES
                     IAQFDSIRDRVVIINGVSKGYAMTGWRIGFSASAKWIAKAAAKLQGQQTSGVCTIAQK
                     AATAAFNSDGQTTRQMCEAFKRRRDLFVKGISGINGFKINVPQGAFYLFPDITHFLGL
                     QFNEQVVATADDFAMYLLNNAHVAVVSGSAFGSPSNIRLSYATSDEKLSMAVERIA"
BASE COUNT         2196 a         1800 c         1961 g         2485 t
ORIGIN      
        1 ttgcttcctg agcgcttaca actgtagcga gaagcatggt tttacccatt gttagcacaa
       61 ccgagccatg agcctgcctg gccaactttc ccgtctccag cgtgatggtt cgaccatcgc
      121 ccaactcgat tgttttttta aaaatattca tctgcttgtg tttatttaat ttgttgtgtt
      181 tcaaaaaaag gttttaagaa gttctcgtta aaggtagaaa tactatatac ttgtcatttt
      241 aacctttgcc aatcatgata gggtcggtat ggataatcct catggtcgca cagctataac
      301 cgagtatttg tgcggtatgg gaaaatcatt ttaaaaaata ttgttctgcc gtgatattct
      361 ggtttaaaaa atttcgttta gaaatgctag ctatattaaa aaaagtagag gcaatttact
      421 cttccatttt tgatttaagg ggtggattgt aaaaaaggca attttttaaa attgcctttt
      481 taagttttac tttctcaaat tcagcgcttt aactacctcg cgatacctgt caatatcttt
      541 ctccttcaag tagtccagca accgcctccg ttgaccaacc atctttaaca gcgaacgctg
      601 tgtagcgtaa tcttttctgt gagttttaag atgttctgtg agatggttaa tgcggtagga
      661 aaacaatgca atctggcttt ccgaagaacc agtgttggta tcggacttac catactccct
      721 aaaaatttcc tttttctttt ctacggtcaa atagttcata gcactaataa ttagtgagta
      781 gatatacact tattttgagg tgcaaagata ggagtttttt tacattttgc ctatacactt
      841 aatatttttt tgaaaagttc tgaagatgat ggatatctga aaaaattgaa ggacaatgga
      901 taaggagaat ttgtttggat ttggctgata aatccttcaa ccaggctctg gacaggctaa
      961 aatcttctag ctttagctat tcctgtcccc attttaaact ttttcagggt gctcgcctct
     1021 tagaaggaga attttcccat ggcgtaagtg aaattgatgg tgaaccaccc gtgtaggaca
     1081 acttgcacaa acctgcatga acaaccggcc tttacctgca aactcggtga agccaaaata
     1141 cgaatgttcc attatattga ccccgaagcc taaaagtagg gtaaaggtaa aagtaatcag
     1201 taaccggaaa cctaataatt ttgctatgct aaagctctac gtcgaaagtt aagacttgtt
     1261 tcgttttgct gcatcaaccc taattagctc cctgccaggg ttgagaagca accgatcctg
     1321 gcagctggtt agtattcaag gcaaaggttt ttctccttca ccaccttttt taggtttata
     1381 agtgcatacc gcattcttcc taaggcagtg ttaatgctca cgttggtgat atcggctatt
     1441 tccttgaaac tcaagtcggc atagtaccgg agcattacca cctcacgctg gctggggggt
     1501 agcccatcta ccatcatcct tacttcagcg attacacgtt cgtaaaccat tcgttgttcc
     1561 gcattcattt cagaaaaacg gggcgtgttg agcaggtcaa ctccatattc ctcgttagaa
     1621 acctcacggt aatggctttt ctgtctgaag taatcaataa ccaggttatg cgctattcgc
     1681 agcagccacg attgaaaccg gttgctctcc gaatacttgc ccccccttag cgactttatg
     1741 gccttcacaa aagtctcctg catcaagtca tctgccagat gcgagttatg aaccattagt
     1801 aaaatgtagg tgtaaactct acgttcgtgc ctcttaatta gcacctccat tgcagcattg
     1861 ctgccttcct taaattccga tagaagctcc tggtcggaac gattgtttgc attcacagca
     1921 acctcccatg tattggtaat cgggtagagc ttcttccaat aggcaggtgt gatttaggtt
     1981 agttaactta agttacaatc gtttgccaaa aaaagtattt ttttttgata tgtgcaagtt
     2041 gaacccattt ttttattatc acgtggctgt ttattttctt actttttttt gagggaaggt
     2101 tcaaaggaaa ttcctcagca aaattactgg tttggtttgt ttcaggttta cagatgagaa
     2161 aagttgaggt tattccagct tgttttgcga atagctgcta cctttgccgg ttaatatttt
     2221 ttttaaatga cacgtagtgt tgaaaatgtt atttcggtaa agggtgcacg ggttcataat
     2281 cttaaaaata tcagcctaga cattccccgc aacgccctgg tagtaataac gggagtatcg
     2341 ggttcgggca agtcatctct ggcatttgat accatatatg ccgaaggtca gcgtcgctat
     2401 gtggaaagcc tctctgccta cgcacggcaa tttcttggac gtatggctaa gccagaggtt
     2461 gatctgatta gcggaatccc cccggcaatt gccattgagc agaaggttaa tacacgaaat
     2521 ccccgctcta cggttggaac atcaaccgaa atatacgact accttaagct gctctttgcc
     2581 cgaattggaa gaacatactc ccccgtatcg gggaaagagg tgaaaaagca ttctgtcagc
     2641 gatgtggtta acttcatcag ctcctttaac gagggaacca aaatgatgct tttagcaccc
     2701 cttacaccga atggggatat gaagcagaag ctggaagctc ttgctcacga aggctttaca
     2761 cgtgttgagg ttggaaatca gatagttcgg attgatgagc aaaaattcag ggaggtggtt
     2821 gacctttcgg gatcggagtt gcggctggtt atcgatcgtt ttgccgcagc cgctgatgat
     2881 gaattcctta gccggttggc cgactcggtt cagactgcct ttgcagaggg gaaaggcttt
     2941 tgcctgttga aagtattcga cgacaatggg agctatagcc tggagcaatt ctccagcctc
     3001 ttcgagatgg acgggattaa gtttgaagaa cccaccgagc acctgttcag ttttaatagc
     3061 cctttgggtg cttgccctac ttgtgagggc tatgggaaaa ttgtaggaat cgatgaaaat
     3121 ctggtcatcc ctaaccgtag tctttctgtt tacgatgatg caattgcctg ctggaagggt
     3181 gaaagtatga gctggtataa ggatagggtt gtggaaaatg ccagccgata cggaatacca
     3241 attcataaac catactttca gctttccgag gagcagaaat cgctactttg ggaaggaact
     3301 cctcatttca ctggaattaa tgactttttc agagagctgg agggaaaaaa gtataagata
     3361 cagtatcggg ttttgctttc ccggttcatg ggaaaaacta cctgcccaac ctgcagggga
     3421 ggaagacttc gtcgtgaggc aacttttgtc aaggtgaacg gctataccat acaggaattg
     3481 gttgagatgc cagtagccga cttggcggaa gttttcaaca atctcgagct caccgaacat
     3541 gaggttgggg tagccaaacg aattcttatc gagctaaaaa atcgcttgaa ctacctggtc
     3601 aacgtggggc ttggatacct cacgctcaac cggctctcct catcactgtc gggaggtgaa
     3661 tcgcagcgta tcaacctggc cacatctttg ggaagcagcc tggtaggttc gctgtatatc
     3721 ctcgatgaac ccagcatcgg gctacatcct cgtgacaccc acctgctcat tgatgttctg
     3781 aagaaactgc gtaacatggg caatacggtg gttgttgttg agcacgatga ggaaatcatc
     3841 agggcttccg attatatcgt tgacattggc ccctatgccg gggttaacgg aggcgaggtt
     3901 gttttcgaag gacccatcga aaaccttgaa aaagccactc aaagcctaac ggccaacttt
     3961 cttttaagca gggagcaaat actcgttccc agggttcgga gaaaatggaa tagctatatt
     4021 gagatttccg gagcccggga aaataaccta aagggtattg atgttaaatt tccgcttagc
     4081 tgtctggttg tagttacagg tgtaagcgga tcgggtaaat cgagcctggt ggacgatatt
     4141 ttataccctg cccttctgaa gcagctaacg ggtgctggtc cccgtgccgg acagtttgat
     4201 tcgctaggtg gcgactggaa gctaatttca ggggttgagc tggtagatca gaatccaatt
     4261 ggccgatcgt cgcggagcaa tccggttacc tacatcaagg catgggatga gattcgtaag
     4321 cttttatccg aacaacccta cgcaaaacga aatgggtata agcccgctca tttctccttt
     4381 aatgtcgagg gtggacgatg cgaggagtgc cagggtgaag gtgttattca tgttgagatg
     4441 cagtttatgg ccgatttgac cttggtttgc gagagctgta aggggaaacg ttttaaggat
     4501 gatatcctgg aggtgaggta caacggagcc aacgtttatg acattttaaa catgacggtt
     4561 gatcaggcca ttgaattttt tagcaagggg aaaactttaa ctgagcagaa agttgtggat
     4621 aggctaaaac cgcttcagga tgttgggctg ggctacatac agctgggaca atcgtccagc
     4681 accctcagcg gtggggaaag ccagcgggtg aaacttgcct ctttccttca aaaggatgcg
     4741 tccattcgcc cgcttctttt tatttttgac gaaccaacca ctggtcttca tttcaacgat
     4801 attcgccagt tgctgaatgc ctttgacgcc ctgctggcta aaggacacag cctggtcata
     4861 gttgaacata accttgaagt gataaaaagt gccgactggg tagtagattt gggcccggaa
     4921 ggcgggcagg agggaggttt gctggttttt gccgggactc ccgaagatct tgctgctaac
     4981 gaggcgtccc atacgggtgt atttctaaaa aagaagctga atggttaata aaaaaagccg
     5041 catgtagcgg ctttgtacag tcttagctgt agctactcgg ccttcaaccc gaacatgatt
     5101 gctacgcaac cacttgccaa ctccgagcca gaatttcccg atgaaggcac tctcaccaaa
     5161 ataatcagct gctgggttgc ctcaaccttg aagttccatg atgtggcata gttatttttg
     5221 gcattggagt atagcaccct gcggtcaacg tccatcacca gaaattctat tttagccagg
     5281 gattcggagc cacaaattga tattctgtat tcctggcctg cataaaaggt tttaaatagc
     5341 tcggcgtcct caccctcggt tagaattgct gcgttgtaat tcccatcatg aacgtacgga
     5401 gccaactcca acttgcaaat tttttttgca aagtttttac attgggcttg tgtgctgtgg
     5461 ggttgaagta gcagtagccc tagaataaga attgtagtgg taaatgattt cataccggaa
     5521 tgtgttatgc tatgctgtgt actttgtacg caattcctta acttttgttt taagcttgtt
     5581 gaaaacatca gggttaacgg taatgacatt ttttgaacgc aggatggttg ccccgttgtt
     5641 gctagcggga acaacttcaa cccgagatgc agaaatttga atgtcataaa atatttcgct
     5701 tagcttttgc atgtcggagt aaatttccat aacatcagcg ttatcggaat tctgctttaa
     5761 aaggcttaaa acggtttcaa gggaaaactt ctgatcggca attctatcca ccagctcatt
     5821 atccatcttc ttttcatcgc taaccagcga ggtggccagg taaagcccct ccaaccatcc
     5881 gcctaccaga attatagatc caatagcaac ccggtcatct tcctccagga tggagttggt
     5941 gttaaggaat gtttcggata tggtttccat tattacatcg cggttgttga tgttttcttc
     6001 aagccgttga attaccgagt tatcaatggc attcagaatg cctagcccat ctgccatttt
     6061 ctttgatgca ttcatcaggt taatcgtggc ctgggtttga tcgaacaggc tggcgtagct
     6121 taagtcggtg ctgtagattc ccaggtttaa cgccatgctt ttactggtgt tgtagtgcgc
     6181 aacgttttca accgggttta gcaggtcttc attgtatact gcgcctgacc gttttagtat
     6241 tagggccgtt tcgagtgggg agggcaggga gtagaatatt tgcttaaccg attggatgct
     6301 ttgagaaatc acgctatcag cggcaagttc atcctccgtg acttttaacg atttgtccga
     6361 gggtttctcc gatttgcagg ctaccccgcc tactatcagc agcagtccta ataactttac
     6421 tattcgcttg acgaaacctg tagccatatc accaactttt aattgtcaat tttacccaca
     6481 ttttcagtaa gtaaagataa aataattttt tactatattc ctatagaaat tcattgttga
     6541 attcaaataa ttgcaaaaaa gttgataacc agggttgatt ttcaaccatt ttgttgtttg
     6601 gatggaatca agctatgtcg gtccagtaaa tgcaacatgc agaaaaataa ttcctctgct
     6661 taaggcttaa aaaatttcgg ctacctcctt ttttaggaaa tcaatagcaa gcgtagtggg
     6721 gttagtatca agctccacgt aggtttcaat tattttttgg ctcaaggcca tgctggtttc
     6781 gttggggttt atcctgtcgg cacaaacgtt gataagctta ataatgttct ccttggcatt
     6841 tttaaccatc tcccaggctt ccttgcttac gtagagctgc tgcgataggt tgtgctccca
     6901 ttcagccctt atggtgccca gcaatgccgt gtgcaggtca gaagccagca tccctttttt
     6961 tgtagttctt ggcagcaacg attcgggtga tattcgttcg aggaaaagca caagcctctc
     7021 gtaggcttgc agcctcactg gggtggttat ctttatgtta ttaaacatca gctcagattt
     7081 cctgcggttg gtttcgctct taatcatggc cgatatggca aggtatcccg ccagaaaaac
     7141 cattagagcg ggcagcacta tcttcagaat ttctgctagc atgggttagg attttaaggg
     7201 aaagtttgac tatttttgag ttcttaaaat tacaaaaaga attttgaatt tattttaaat
     7261 ctatacggtt atggagaaag tatcttctcg aatagcagca ttggaggaat ctcaaaccat
     7321 tgccatgtcg caaaaatcac gggagttggc ggcacaggga attgatgtgg ttaacctcag
     7381 cttgggtgaa cccgattttt ttactcccga ttttattaag gaggccgcaa agcgcgctat
     7441 tgatgagaac ttttcgtttt accccccggt ttcggggtac aaggacttgc tggaggcgat
     7501 atgcggaaag ctgaagcggg agaatgatct tacctttacc cccgaccagg ttgttgtttc
     7561 ggctggtgct aagcatagcc tgtcaaatgt gctaatgtgc gttgttgaaa aggatgatga
     7621 agtggttgtc ccagcaccct actgggttag ctacgttgag cttattaagc tggccgaagg
     7681 gcggagcgtt atagttccaa ccaccattga gaatgatttt aagattaccc ctcagcagct
     7741 tgaggctgca ataactccaa aaaccagggc atttctcttt tgctcccctt caaaccccac
     7801 aggaagcgtt tactccaagg ctgaacttga ggccattgcc agggttattg agcagcaccc
     7861 aaatatcata gtaatttccg atgaaattta cgagcatatc aacttcgtgg gaaaacatga
     7921 gagcattgcc cagtttgata gcattcgcga cagggtggtc atcattaacg gggtgtcgaa
     7981 aggctacgcc atgaccggtt ggcggattgg attttctgcg tcggctaaat ggatagccaa
     8041 ggctgctgcc aagctacagg gacaacaaac ttcgggagta tgcaccattg cccaaaaagc
     8101 tgccactgct gcctttaact ccgatggtca aaccacccgc caaatgtgcg aggcgtttaa
     8161 aagacgccgc gacctgtttg taaagggaat ttcgggtatc aacggtttca aaataaatgt
     8221 tcctcagggc gcattctacc tcttccccga cattacgcac ttcctgggtt tgcaatttaa
     8281 tgagcaggtt gttgctactg ctgacgattt tgccatgtat ctgctaaaca acgctcatgt
     8341 tgctgttgta tcgggctcgg cttttggctc gccctccaat attcgtttgt cctatgctac
     8401 gtctgacgaa aaacttagca tggctgttga gcgcattgcc ca
//
LOCUS       DAOPHX010000113         7997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_11463_length_7997_cov_7.446109, whole genome shotgun sequence.
ACCESSION   DAOPHX010000113 DAOPHX010000000
VERSION     DAOPHX010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7997
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11463_length_7997_cov_7.446109"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(610..1458)
                     /locus_tag="PK502_08510"
     CDS             complement(610..1458)
                     /locus_tag="PK502_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPH00897.1"
                     /translation="MVDAIIEFFSYGFVRNAIIASALISILVGFIGTYVVSRRLVFLS
                     GGITHASFGGIGIAWYLGWNPLLGAAIFSIVSAVGIEYLTGEKRLREDSAIGMLWALG
                     MALGVFFVFLTPGYAPDLMSYLFGNILTISTSDNLMLLVLSLVTALFFTLFYYPILFL
                     SFDEEYVKTQHVKARVINFTLKILVALAIVLSIRAIGIILVISLLTIPANIANLFTKR
                     FGNIIWLSALICFGSTILGIILSYRLNVPSGATIVIALALIYLLTRFYFHLKGAIFRD
                     SLRKGL"
     gene            complement(1531..2316)
                     /locus_tag="PK502_08515"
     CDS             complement(1531..2316)
                     /locus_tag="PK502_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPH00898.1"
                     /translation="MMSNEHSRIVEIRNVTAGYGRDVVLKNVSLDIYDDDFIGIIGPN
                     GGGKTTFLKLLIGQVKPFSGEVSYSTSGKQLPIGYLPQAGMLDKNFPITVMEVVLSGL
                     LSQKGLTGRYSKAQRQLANDYLRKTGIEALKHRPIGELSGGQLQRTLLCRALISEPKL
                     LILDEPNTYVDTNFEGELYEILKELNQRMAIIIVSHDLGTITSYVKTIACINRTLFYH
                     KSNQLSTELLAAYNCPIQIIAHGPVPHTVLLEHEHHADNNLTE"
     gene            complement(2306..3193)
                     /locus_tag="PK502_08520"
     CDS             complement(2306..3193)
                     /locus_tag="PK502_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPH00899.1"
                     /translation="MRRISGIMVFLVVVLGLMLPACTEKKHTTSKPIVMVSIQPIKYF
                     VEQIADTLVDVRVMVPPGSSPEMFEPTPGQMVDLSNADIYFAIGLLDFEKGLEPKLKD
                     QLKVRYVNLSGKADLIKEGTDEHDSVGGHHHGVDPHIWMSVEEVRKMSMQILQNLQQL
                     YPAHSDFFSNNYIAFGSKLDSLKEQVNRILGKSSNRAFLVYHPALGYYAREFGLRQIS
                     IEQEGKNPSLKGVVDVVDQARRLGVSAILSQSQFDIRNAQVIADELNLRVVKVDPLAE
                     DWENSMLHIARAISGEHDE"
     gene            3415..5985
                     /locus_tag="PK502_08525"
     CDS             3415..5985
                     /locus_tag="PK502_08525"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPH00900.1"
                     /translation="MNSRTKSSLILLLLLWTAVFFASMGHFTAYSQEKDSLIQLLSDA
                     KGKQKLEILFKLSELTSQTEEGIKFAEEALAVAKKMDRVSLIEAYENMAIAYSENFLD
                     EAALSYFKKAYQLSLEYGYIPGLLKACKGISTSYFYLDSINLSTKYSNELLAAAEKVD
                     SLNYEADAYFWLGRIKQAEGQTDSALMFIGKALKLREKLGNLKEVAQTYNGLGGLYFD
                     DAQYEKAIECYKKEIEIKEAIHDKPDYLALAYLNLGRAHIALSNFQLALEQYQKALRT
                     FESINNEKGIAVSNSGIGMIYENLSQSVMATEENEVNYTKALEYHKNALRLFRKMGQR
                     KEEGQTLQNIGNVFSRLATNRFVARFGEEWEDSLYKIKQDQILASFDSSLIYYQYALD
                     IFNQINDEREIVKVNTNLGSTYSWARDWPKATRYISQAVGICRRNGYQYELSAALYAQ
                     GESYYRQGNLAGAEVSFLECVEISTRLGLKETLRYSYNRLSKLYEQKGDMAKALANLK
                     LSVRIKDEIFTEKSQKIITEMQTKYETEKKEQELKLMKNQDELQKSIIQRQKLMIIGA
                     IVGALLILMVALLMFKMFRDKQRANRILQEKNALITSQKKEITDSIQYASRIQSAVLP
                     TSSLLAEALPEHFVLFKPRDIVSGDFYWMTRKNEKVVLVAADCTGHGVPGAFMSMLGV
                     SFLYEIVNKENVLQPAEILNQLRIHIKTTLSQTGKQNEQKDGMDISLCVIDRPGNVLE
                     WAGAYNSLYQIRGGELTEYKADKMPVAVHVSDSNSFTNHQIEIQHGDTFYMFSDGYAD
                     QFGGTDGKKFMSKKFKELLVGIWNKPMDEQGKIINETHLQWKGEYDQVDDILVIGFRV
                     "
     assembly_gap    6005..6104
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6130..7443)
                     /locus_tag="PK502_08530"
     CDS             complement(6130..7443)
                     /locus_tag="PK502_08530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPH00901.1"
                     /translation="MGNHLKGFRHFTTMEIPEYFQNKNFSPSIQELPTKKLSIVVVIP
                     SFNEADLKDSLMALASCSKPRGDVEVIVVVNHPENSSAEVVTTSEQSLQVVAEADSCW
                     GSSSFRFYAIKAFNLPQKQAGVGLARQIGMDEAAYRLFLAKQPGGIIACFDADATCEV
                     NYLVELENFWAANPDIHACSIRFEHPIQGNNFPQEVYDGIAQYELHLRYYNQALRYTG
                     FPFAFHTVGSSMACTAEAYVKFGGMNRHQAGEDFYFLQKIIPHGHFAELNTTCIYPSP
                     RASNRVPFGTGKSMAMFLENPGNEIQTYQLESFFPLQMLFGKMQSLYQLDVGQIDCFI
                     DSMPAPLKLFLKQNNATEKIYSISRNTASPGSFRKRFFLWFDAFLMLKYLNFVHNRFF
                     KRQPVKHEAIRLAGIIGLRVDERWPVEKILVKYRELEANKFNVNP"
     gene            complement(7490..>7997)
                     /locus_tag="PK502_08535"
     CDS             complement(7490..>7997)
                     /locus_tag="PK502_08535"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPH00902.1"
                     /translation="SEASAALSEGLSVAWVNMDTLLNHYDMYFDMRKELENNSRNLEA
                     QLNSKSKAFEQDAMDFQNKVQKGLVTRSEAQQLQTSLANREQELYKLRDDMQMQLAEE
                     EQVKLRQIHYSITEYLKKYNADKGYHIILSSNFGGPLLYGHPALDITSEVIGGINQEY
                     AANHKTDK"
BASE COUNT         2192 a         1735 c         1852 g         2118 t
ORIGIN      
        1 tttgttgttt ctcagtatta cctaaataag taatattaaa tggcactttg tgtagtatcc
       61 tggtgccctt tgtggtaaaa aattaacgta aataagtgat tttcttacta ttgcatactt
      121 aatccaactt tacagaaaca aggttacgtg gcgaatatta accacaaagt tctcaaagca
      181 tgcacgaaga acacagagaa aaaatgaaag agaatgataa actttaatgt tatttcatta
      241 aaggatggaa ttagaagggt tataaatggt actttgtgaa ctttgtgtag catcctggtg
      301 ccctttgtgg taaaaaaata acgtaaataa ttgactttct tactattgta tacttaatcc
      361 aactttacaa aaacaaggtt acgtggcgcc aacaaaaaag ctactatata aaatgaaaat
      421 atctattacc taaaaattta gggttttggg ttaatgaata cattcaaagc tgtgcaatag
      481 ccaatcatca aagagcttga taagcaaatg agggcgtgta gtttttttag aagaatggca
      541 tagaaaactg gccaggttaa tctggttatc cgattagttg taggtgtgaa ttatgtcgta
      601 gtaaggtgtt tacaagcctt tccttagcga atccctaaag atagctcctt taaggtgaaa
      661 gtagaatcgt gttaaaaggt agattagtgc cagggcgatt actattgttg ctcctgaggg
      721 aacgttcagc ctgtatgaaa gaattattcc cagaatagtt gagccgaagc agattaacgc
      781 cgatagccag atgatgttcc caaaacgctt ggtaaacagg ttagcaatgt tggcaggtat
      841 ggttaatagc gaaatcacaa ggataattcc aatagccctg atggagagaa caatggcaag
      901 ggccacaaga attttaagcg taaagttgat gaccctggct tttacatgct gggttttaac
      961 atactcctcg tcaaaggaaa gaaaaagaat ggggtagtag aaaagggtaa aaaacaaggc
     1021 ggtaacaagt gatagcacca gcagcatcag gttgtcgctg gtagaaatgg tgagaatatt
     1081 gccgaaaagg tacgacatca ggtcgggggc atagccgggg gttagaaaaa cgaaaaacac
     1141 accaagcgcc atgcccaacg cccagagcat cccaatagcc gaatcctcgc ggagacgctt
     1201 ttcgccggta aggtactcaa tacccacggc cgaaacaatg gagaaaatgg cagcgccaag
     1261 cagcggattc catcccaggt accaggcaat tcctattcct ccgaaggagg catgagtaat
     1321 tcccccgcta aggaatacaa gcctgcgcga tacaacgtat gttccaataa aaccaaccag
     1381 tatggagata agcgcagagg cgataattgc attccgcaca aatccgtagc taaagaattc
     1441 tatgattgcg tcaaccattt tgtttttacc taattgttga ttatccgcaa agtcggcgga
     1501 agtttaattg tggcctttag tttatgttta ctattctgtt aagttgttat cggcatggtg
     1561 ttcgtgctcc agcaggacgg tatggggcac cggtccgtgt gcaattatct gaatgggaca
     1621 gttgtaggct gccagcagct cagtgcttaa ttggttcgac ttgtggtaga atagggttcg
     1681 gttgatgcag gcaatggttt taacatagga ggtaatggtt cctaaatcgt gcgaaacgat
     1741 tatgatggcc attcgctggt tcagctcctt tagtatttcg tagagttccc cctcaaaatt
     1801 ggtatcaacg tatgtgttgg gctcgtcgag aataagtagt tttggctcgg aaattagtgc
     1861 tcggcagagc aaggttcgtt gaagctgtcc acccgatagc tcccctattg gtcggtgttt
     1921 cagcgcttca attcctgtct ttcttaggta gtcattggcc agctggcgct gagccttgct
     1981 gtaacgaccg gtaagccctt tttgggatag aagtccggaa agaactacct ccataacggt
     2041 tatgggaaaa tttttatcga gcatacctgc ctgtggcagg taaccaattg gaagttgttt
     2101 tccactggta gagtagctta cctcaccgct gaagggtttt acttgcccta tcagcagctt
     2161 caggaaagtt gtcttgcccc cgccattcgg cccaattatc ccaataaaat cgtcgtcgta
     2221 gatgtcgagg ctgacatttt tcaacaccac gtcacgccca tacccggcgg taacattcct
     2281 aatctctact attcttgaat gttcgttact catcatgctc gcctgaaatt gcccttgcta
     2341 tatgcaacat gctgttttcc cagtcttccg ctagcggatc aaccttaaca acccttaggt
     2401 taagctcatc ggctatgacc tgggcattgc gaatatcgaa ctggctttgg gaaagaatgg
     2461 ccgatacgcc taacctgcga gcctggtcaa caacatcgac cacaccctta agcgatggat
     2521 tttttccctc ctgctcaatg ctaatttgcc tgaggccaaa ctcccgtgca tagtaaccca
     2581 gtgcaggatg gtaaaccaaa aatgcacgat tggaagattt tccaagtatc ctgttaacct
     2641 gttccttcag gctatccagc ttgctgccaa aagcaatgta gttgttgcta aaaaagtcgc
     2701 tatgggcagg ataaagctgc tggaggttct gtaggatttg catggacatc ttccgcacct
     2761 cctcaaccga catccagata tgggggtcaa ccccatgatg gtgtccgcct acagaatcat
     2821 gctcgtcggt tccttccttt atcaggtcag cttttcccga taggtttaca taccttactt
     2881 tcagctggtc ctttagttta ggttccagac ctttttcaaa atcgagaagc ccgatggcaa
     2941 agtatatgtc ggcgttactt aaatcgacca tttgtcccgg ggttggctca aacatttcgg
     3001 ggctgctgcc cggaggaacc attaccctaa catccaccag cgtgtcggca atttgctcaa
     3061 caaagtactt aatgggttga atgctgacca ttacaatagg cttgctggtg gtatgcttct
     3121 tttctgtgca ggccggtagc attaacccta ataccacgac caaaaaaacc atgattccac
     3181 taatccttcg catttcttgt taatttagca tctacacttc gctaccctgc ttccaggttc
     3241 ggttgggctg aacggaccgg ttgagaccta ccggattgtt ttgaagatga ccagaacccg
     3301 tgtaaacatc aaacaaaccc aaattaagca aatttgttta ccagacatct tctttttaaa
     3361 agttttgcat tttggttaaa cttttgttaa tttgccagtt aaagccttcc cccgatgaac
     3421 agcagaacca agagtagcct gattctacta cttttacttt ggacagcagt tttctttgct
     3481 agcatgggcc attttactgc gtatagccag gaaaaggata gcctgattca gctgctttcg
     3541 gacgctaaag gtaagcaaaa gctcgagatt ctcttcaagc tttctgagct aacctcccaa
     3601 accgaggagg ggataaaatt tgccgaggaa gcgctggctg ttgccaaaaa aatggatagg
     3661 gtttcgctaa ttgaagccta tgagaatatg gccattgcct actcggagaa ttttcttgac
     3721 gaagcagccc tttcctactt caaaaaagcc taccagctaa gcctcgaata tggctacatc
     3781 cccggcctgc taaaagcctg caagggtatt tctacctcat acttttatct cgattccatt
     3841 aacctttcta ctaaatactc gaatgaactt ttagctgctg ctgaaaaggt tgacagcttg
     3901 aactatgagg cagatgctta cttctggctg ggtaggataa agcaggctga agggcaaacc
     3961 gattctgcgc ttatgtttat cggcaaagcg ctaaagttaa gggaaaagtt gggcaattta
     4021 aaagaggtcg ctcaaaccta caacgggttg ggcgggcttt actttgatga tgcgcagtat
     4081 gaaaaggcca tagaatgcta taaaaaggag attgaaatca aggaggccat tcacgataag
     4141 cccgactacc tggcactggc atatttaaac ctgggacgtg cgcatattgc gttaagcaac
     4201 tttcagctgg cactggagca gtaccaaaag gcactccgaa ctttcgaaag catcaacaac
     4261 gaaaagggca ttgccgtttc caattccggt attggcatga tttacgagaa tctctcgcaa
     4321 agcgtcatgg ccactgagga aaacgaagtg aactacacca aggcgctaga gtaccataaa
     4381 aatgcgttgc gcctgtttcg taaaatgggg cagcgaaagg aggaggggca aacactccag
     4441 aacataggca atgttttctc acgacttgca accaaccgtt ttgttgcccg gttcggggag
     4501 gaatgggagg attctctcta caagattaag caagatcaaa ttctcgcctc gttcgatagc
     4561 tcacttattt actaccagta cgcacttgac attttcaatc aaataaatga tgagcgggaa
     4621 attgtaaagg taaacaccaa cttaggaagc acttacagct gggccaggga ttggccaaaa
     4681 gcaacccgat acatctccca ggctgttggt atctgccgtc gtaatggcta ccagtatgaa
     4741 ctttcagctg cgctttatgc tcagggcgaa agctactacc gccagggcaa tctggccggg
     4801 gctgaggttt catttctgga gtgcgttgaa atatctacca ggttaggtct gaaagaaact
     4861 cttcgctaca gctacaacag gctatcaaaa ctttacgaac aaaagggaga catggccaag
     4921 gcgttggcca atcttaaact ttcggttaga attaaagacg agattttcac ggagaaaagc
     4981 cagaaaatca taaccgaaat gcaaaccaag tacgaaactg aaaagaagga gcaggagtta
     5041 aagctgatga aaaaccagga tgagctccaa aagtccatca ttcaacgtca gaagttgatg
     5101 ataatcggcg caattgtcgg agccctgctg attcttatgg ttgccctcct gatgtttaag
     5161 atgttccgcg acaagcaaag ggccaacagg atacttcagg agaagaatgc gttgataacc
     5221 agccagaaaa aggagattac cgatagcatt cagtatgcaa gccgcataca atcagccgtt
     5281 cttcctacct cttcacttct ggccgaagcc ctgcctgagc attttgtact tttcaagcca
     5341 agagatattg ttagcggcga tttctactgg atgacccgga aaaatgaaaa ggttgttctg
     5401 gttgctgccg actgcaccgg ccacggagta ccaggggctt ttatgagtat gcttggggtt
     5461 tcatttctct acgagatagt taacaaggag aatgtgctgc aacctgcaga aattctaaac
     5521 cagcttcgca tccatatcaa aacaaccctt tcacaaacag ggaagcagaa cgagcaaaaa
     5581 gatggaatgg acatttccct ttgtgtaatc gaccgcccgg gcaatgtttt ggagtgggca
     5641 ggtgcctata attccctcta ccaaattcgg ggcggagagc taaccgagta caaagccgac
     5701 aagatgcccg tggctgttca cgtaagcgat agcaactcct ttaccaacca tcaaatcgaa
     5761 attcagcatg gcgatacctt ctacatgttt tccgatggct atgctgacca gtttggcgga
     5821 acagatggta aaaagtttat gtcgaaaaaa ttcaaggagc tgctggtagg aatttggaat
     5881 aagcccatgg atgaacaagg caaaattatt aacgaaaccc accttcagtg gaagggcgaa
     5941 tacgatcagg ttgacgacat cctggtcata gggttcaggg tttgaaggta accggtagag
     6001 ggcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntctcac ggctttttac
     6121 cttttacccc tatgggttta cgttgaattt attggcctcc agctcgcggt atttaaccag
     6181 aattttttca actggccaac gttcatctac ccgaaggcca attataccgg caagcctgat
     6241 agcctcgtgc ttcaccggtt gccgtttgaa gaaacggttg tgaacgaagt tcaggtactt
     6301 caacatcaaa aaggcatcaa accagaggaa aaatcttttt ctgaacgaac cgggagatgc
     6361 cgtattacgg ctaatgctat aaatcttctc ggttgcattg ttttgcttca ggaatagttt
     6421 taatggggca ggcattgaat ctatgaagca gtctatttgg ccaacatcta gctggtagag
     6481 cgattgcatt ttaccaaaga gcatctgtag cgggaagaac gattcaagct ggtaggtttg
     6541 gatttcatta ccgggatttt ccaggaacat ggccattgat ttacccgttc caaagggcac
     6601 cctgttcgac gcccggggtg aagggtaaat gcaagtagtg tttaactcgg caaaatgccc
     6661 atggggaatt attttctgca ggaagtagaa gtcctctcct gcctggtggc ggttcatgcc
     6721 gccaaactta acgtaagctt ctgcggtgca ggccatggat gagcctactg tgtggaaggc
     6781 aaaagggaat ccggtgtagc gcaatgcctg gttgtagtag cgcaaatgca gctcgtattg
     6841 agcaatgcca tcatacacct cctgtggaaa attattaccc tgaatgggat gctcaaaacg
     6901 gatagagcag gcatgtatgt cagggttggc tgcccaaaag ttttccagct caaccaggta
     6961 gtttacctcg caggtggcat cggcatcgaa acaggcaata atgcctcctg gttgtttagc
     7021 taggaacaag cggtatgcgg cttcatccat acctatctgt cgtgctaacc ccactccggc
     7081 ctgcttttgg gggaggttaa aggctttaat ggcataaaac ctgaagctgc tgctgcccca
     7141 acaactatct gcctcagcca caacctgaag cgattgttct gaggttgtta ccacctcggc
     7201 cgaagaattt tcgggatggt taaccaccac aataacctca acatctccac gtggtttgct
     7261 gcagctcgca agtgccatta acgaatcctt cagatctgcc tcattgaagg aaggtattac
     7321 aaccactatg gataactttt ttgttggcag ctcctgaatg gatggggaaa agttcttgtt
     7381 ttgaaagtat tcggggattt ccattgttgt aaaatggcga aaccccttca aatggttgcc
     7441 catcgaaagg gtttcggttg gtttaggttt agtttaggta ggttataaat tacttgtcag
     7501 ttttatggtt ggcagcatat tcctggttaa tgccgcctat cacctcgctg gttatgtcca
     7561 gtgccggatg accgtaaagc aacggaccgc cgaagttact gctaagaata atgtggtaac
     7621 ccttatcggc attatacttc ttgaggtatt cggtaatact gtagtgtatc tggcgtagct
     7681 taacctgctc ttcttcggca agctgcatct gcatgtcgtc gcgcagcttg taaagttcct
     7741 gctcccggtt ggccagggag gtttgcagtt gctgggcttc ggagcgtgta accagcccct
     7801 tttgaacctt attctggaaa tccatggcat cctgctcaaa ggcttttgat tttgagttca
     7861 gctgtgcctc caggtttcgg ctgttgttct ccagctcctt ccgcatgtcg aagtacatat
     7921 cgtagtggtt gagaagggta tccatgttga cccaggcaac tgatagtcct tcagaaaggg
     7981 ctgcacttgc ttctgat
//
LOCUS       DAOPHX010000114         7787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_11911_length_7787_cov_6.796172, whole genome shotgun sequence.
ACCESSION   DAOPHX010000114 DAOPHX010000000
VERSION     DAOPHX010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11911_length_7787_cov_6.796172"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="PK502_08540"
     CDS             <1..968
                     /locus_tag="PK502_08540"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPH00903.1"
                     /translation="SIIKEIDASDTLNFMVSDNYMQEAIDRGWYNPASGKPFIWQEAY
                     APLPAEYATGRFWLFYQTFAPNLKEWPNRSAEGNPYKGITPYYQIVEPISLYPFSTKP
                     EKKISVQDVMAFQRSTFEGTIYDMTNDPSWLVPNGKGGYEKSPLATPFPGSDLRKLLK
                     ITYRRPVARHRGHYGMVLQLRGWLPNEIGGVYWVYLDNPYFSPYVPIYAGNLSVHDSY
                     STYDPNRYDEKSARWAIDFVDNLANLKFQAIAADVRAVRSPFEAKIFENQAAVETEAL
                     RLYKKNPQSAQEYLTGYTNGLMSDVTAMFLGLRNQIITNYTNNHE"
     gene            complement(1061..1939)
                     /locus_tag="PK502_08545"
     CDS             complement(1061..1939)
                     /locus_tag="PK502_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPH00904.1"
                     /translation="MTGKRLFKEIRSYSIIVLGLLLYALSWTAFLLPHKLTGGGVSGI
                     GALVFYATGIPMGYTYFVINIGLILLSIKVLGRGFGVKTIFGVTVGAVLLSVLQVVIK
                     QPVVDDKFMSVIIGGAIAGVGLGIVFTQGGSTGGTDIIAMIINKYRNISPGRVILYCD
                     VIIIGSSYFALLDLDPAGRIETIVYGYVAMAITAYAIDATLSGSKQSVQIFVFSKKYQ
                     EIADRITGDLHRGVTVVDGVGWFSKEPQKVLITLVRKYESNDVYKIIKEIDPEAFLSV
                     STVMGVYGRGFDVIKH"
     gene            complement(1957..2361)
                     /locus_tag="PK502_08550"
     CDS             complement(1957..2361)
                     /locus_tag="PK502_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPH00905.1"
                     /translation="MEKSENKTKSFRDLIVWQKAHALVLNIFQQVKTFPEDQQQMVGD
                     YLCESAMAIATNIVGGYKKKDRDEKLLYLSAAQDALEECRYYIILATDLSLLDTFNAE
                     DLENAIGEASFLLNSYVKSIQRRKEDDERVEI"
     gene            complement(2461..5244)
                     /gene="leuS"
                     /locus_tag="PK502_08555"
     CDS             complement(2461..5244)
                     /gene="leuS"
                     /locus_tag="PK502_08555"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPH00906.1"
                     /translation="MDYNFKAIEEKWQALWQQWNTYRVDVNPNRPKFYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRLQGFNVLHPMGYDAFGLPAEQYAIQTGQHPAITTEN
                     NIQRYRSQLDKIGFSYDWSREFRTCEPSYYKWTQWAFIQMFKHWYNNKTQKAEPIDEL
                     VAEFERNGNQHVDAACSETKIFTASDWRNMDGRQQQELLLSYRLAYLADVMVNWCPAL
                     GTVLANDEVKEGVSIRGGHPVEQRKMRQWCLRISAYAERLLSDLEQLDWTDSLKEIQR
                     NWIGRSEGAEVLFSVADSRQQIKIFTTRPDTIYGATFMVLAPESELLDGLTKPEYADN
                     VEAYCNEVKRKTERERVAEAKKITGQFTGSYAVNPFTNQPIPIWVSDYVLAGYGTGAI
                     MAVPAHDSRDFAFARTFNLPIIQTVVRKGEQPTNPETWEESYDAKDGIVVNSPLIDGL
                     EVKQAITRICEIIEQRGLGKSKVNYRLRDAIFSRQRYWGEPFPIYYKDGLPYPLPEEK
                     LPLELPDVDAFLPTEKGEPPLGRAKNWHTPEGYPYELSTMPGFAGSSAYYLRFMDPHN
                     DSQLVSKEANTYWENVDLYIGGTEHAVGHLIYSRFWNKFLYDLGYVCKSEPFKKLINQ
                     GMIQGRSSFVYRIKGTNTFVSLNLKGSYEVTPIHVDVNIVNNDILDVEAFKKWRPEFS
                     NAEFILEDGKYICGWAVEKMSKSMWNVVNPDTIVEKYGADTLRMYEMFLGPLEQSKPW
                     DTNGIDGVHKFLKKFWRLFHNPANQLEISDDNPTPQELKVLHQTIKKVSDDIERFSFN
                     TGVSAFMICVNELTDLKCNKRQVLEPLLILISPYTPHIAEELWSQMGYTGSICDASWP
                     LFNEELVRESTFTCPISFNGKMRFTLDIPLNLPVAEVEKLVLADSNTQRQLDGKTPKK
                     VVVVPHKIVNIVV"
     gene            complement(5505..>7787)
                     /locus_tag="PK502_08560"
     CDS             complement(5505..>7787)
                     /locus_tag="PK502_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPH00907.1"
                     /translation="SCPVGNLFMAPVVYLQGSFVSGLSAYEAALEVNHWCHEKVTYQP
                     TDERTSGPLTTVRTAFGRCGEESTFTVAAYRSVGIPARQVYTPRWAHTDDNHAWVEVW
                     VDGSWHFLGACEPEPELDMGWFAAPAKRAMMIRTFVFGKYNGQEEKLTESDCFAEINL
                     LPRYAPSKNLSVTVVDEANQPVEAARVEFQIYNYAEFYPIASKVTDKNGLCSITTGLG
                     DMMVYAHRDGKTAFVKVDRSLNEMELKLSSTTSAEATDLLLVPPDEISVTSAADDKVA
                     ANSRRLAVEDSLRGCYIATFIDSLSAVKFAASMGINAEKAWHFLSKSRGNWPEIQNFI
                     TNMEPGETPVGMALLGILAEKDLHDITAATLRSHLKTVDSFPSLLDSRSFNLFDRYIL
                     SPRVGREFITPWREFLQGYFTFDQIGFFRNDPTNLVKWISENIKIDSVENYYKVPISP
                     EGVVRLGIADAYSRDILFVAACRSFGIPARLEPSTKRPQYLMRGSWHDVDFGEREVEA
                     SGWGTLTLSATSGMEIASPSYYKHYTIARFENNRFVTLDFENDAQLSTLPFSLSLREG
                     LYRLVCGTRDQNGAVSCRVKYFSIYPDEKTLVTLEFPSSKGKTVVLGNLGDPLIPDIE
                     GSYIKNLGEIGGGGSTVVAIIDPMKEPTRHLVNDLAAIKTQLDKWGGAVVLAIAKDKL
                     PSGFNAQKLLEGVPLPIGVTIGYDSQQSLANALAIACGKPLSTDYPVVAVVNGKGEIT
                     FFSEGYSIGLGEKILKEVGE"
BASE COUNT         2057 a         1954 c         1760 g         2016 t
ORIGIN      
        1 ggagcattat caaggaaatt gatgccagcg acacgctaaa cttcatggtt tcggacaact
       61 acatgcagga ggctattgat aggggttggt ataacccagc ttcaggcaag ccattcatct
      121 ggcaggaagc ctatgcaccc ctacctgctg agtacgcaac cgggagattt tggcttttct
      181 accaaacatt tgccccaaac cttaaggaat ggcccaaccg tagtgctgag ggtaatccct
      241 acaagggaat aaccccttac taccagattg ttgaaccgat ttcgctttac ccgttctcga
      301 ctaaaccgga gaaaaaaatt tccgttcagg atgttatggc atttcagcgg tcaaccttcg
      361 agggaaccat ttacgacatg actaacgatc ctagctggct tgttcccaat ggtaaaggtg
      421 gatatgagaa aagtccgctg gcaacgccat tcccgggtag cgacttacga aagctactaa
      481 agattaccta tcgtcgtccg gttgcccgcc atcgtgggca ttatggaatg gtgctccagc
      541 tacgtggctg gcttccaaat gagattggtg gggtttactg ggtttacctc gacaatccct
      601 actttagccc gtatgtacca atatatgcag gaaacctatc ggttcatgat tcctactcaa
      661 cctatgatcc aaaccggtac gacgagaaat cggcccgatg ggcaattgat tttgttgaca
      721 acctggccaa cctcaaattt caggctattg cagctgacgt cagggcggtc aggtcgcctt
      781 ttgaagccaa aatttttgaa aatcaagctg ctgttgagac ggaagcactt agactgtaca
      841 aaaaaaatcc acaatcagca caggagtacc ttacaggtta caccaacggg ctgatgagcg
      901 atgttacggc aatgtttctt ggtttacgca accagataat cacgaactac accaacaacc
      961 atgaataggt aacactacca gcatgaagaa ttgcagaaag gccggtagct taccggcctt
     1021 tctgcactct atacttccgt ttactcttta accccggttc ttaatgctta attacatcaa
     1081 accccctgcc atatactccc atcacggtag aaaccgaaag gaatgcttcg gggtcgattt
     1141 ccttgattat cttgtaaaca tcatttgatt catattttcg caccagggta ataagcacct
     1201 tttggggctc cttgctaaac cagcctaccc cgtcaaccac cgttactccc ctgtgcaagt
     1261 ccccggtaat cctatcggca atctcctgat attttttgga aaagacaaaa atttgcacgg
     1321 actgcttgct gcccgaaaga gttgcatcaa tagcataagc ggtaattgcc atggctacat
     1381 atccatacac gatagtttca attcttccgg ccggatccag gtcaagcagc gcaaagtatg
     1441 aggatccaat aatgataaca tcgcagtaaa gaataaccct gccagggcta atgttgcggt
     1501 acttattgat tatcatggcg ataatgtcgg taccgcctgt gctgcctccc tgggtaaata
     1561 caatacccaa ccccacaccg gcaatggcac caccaataat aaccgacatg aacttgtcgt
     1621 caactacggg ttgcttgata acaacttgca gaacggaaag aagcaccgcc cccacagtaa
     1681 caccaaatat agttttaacg ccaaaccccc tacccagaac tttaatggaa agtaagatta
     1741 acccaatgtt gatgacgaag taggtgtacc ccattggaat tcccgtggca tagaacacca
     1801 gcgctccaat tccggaaacg ccgcctcccg taagcttgtg tggtagcaag aacgctgtcc
     1861 acgaaagggc atataacagc agccccaaaa caattatgct gtaggagcga atctccttaa
     1921 aaagcctttt ccccgtcatg taccctgaat tgcaggttaa atttcaaccc tttcatcgtc
     1981 ctctttccta cgttgaattg acttaacgta ggagttcagg aggaaactgg cctcgccaat
     2041 tgcgttttcg agatcctctg cattaaaggt atccagcagg ctcagatccg tagctagtat
     2101 aatgtagtag cgacactctt caagggcatc ctgggcagct gaaaggtata ggagtttttc
     2161 atccctgtcc ttctttttat agccacctac aatattagta gcaatagcca tggcgctttc
     2221 gcaaaggtaa tcgcctacca tttgctgctg atcctcagga aaagttttaa cctgctgaaa
     2281 aatattcaac accagagcgt gggccttttg ccatacaatt agatcacgga acgatttggt
     2341 tttgttttca gatttttcca tcttgttaat ctttagaaaa ttagaatttg aaaggttgct
     2401 atatcgctat agctaaacgg gtgaatacga agatacttaa aaaataacat acaacaactt
     2461 ctacacaaca atattaacaa ttttatgggg caccaccacc acttttttag gggttttgcc
     2521 atcaagctgt cgctgggtgt tgctgtcagc caataccagc ttttcaactt cggccaccgg
     2581 caaattcaga gggatgtcaa gagtaaaacg catttttccg ttgaatgaaa tggggcaggt
     2641 aaaggtgctt tccctcacaa gctcctcgtt aaacaatggc cagcttgcat cgcaaatgga
     2701 accggtataa cccatctggc tccacagttc ctcggcaatg tggggggtat agggtgaaat
     2761 tagaatgagt aaaggctcca gcacctgccg tttattacac ttaaggtcgg tgagttcatt
     2821 cacacaaatc atgaaggccg aaacacccgt attgaagctg aagcgctcaa tgtcgtcgga
     2881 aacctttttg atggtttgat gcagcacctt caactcctgc ggtgtaggat tgtcatcgct
     2941 aatctcaagc tggttggctg ggttatggaa taaccgccag aactttttaa ggaatttatg
     3001 caccccgtct ataccattgg tatcccatgg tttgctttgt tccagggggc ccaaaaacat
     3061 ttcgtacatg cgtagggtat ctgcaccgta cttttcaaca attgtatcgg gatttaccac
     3121 gttccacatg cttttgctca tcttctcaac cgcccagccg caaatgtatt tcccatcctc
     3181 gaggataaac tcggcgttgc taaattcagg cctccatttt ttaaatgcct caacatcaag
     3241 aatatcgttg ttcacaatat tcacatcaac atggatgggg gtaacctcgt agcttccctt
     3301 caggttaagg gaaacaaagg tgttagtacc cttaatccgg taaacaaagc tggagcgccc
     3361 ctgtatcatc ccctggttga ttagtttttt aaagggttcg cttttgcaaa cgtagccaag
     3421 gtcatagagg aacttattcc aaaagcggga gtatataagg tggccaacag catgctcggt
     3481 gcccccaatg taaaggtcaa cattttccca gtaggtgttt gcctctttcg acaccagctg
     3541 gctatcgttg tgcgggtcca taaagcgaag gtagtaggcc gacgaaccgg caaatccagg
     3601 catggtgctc agctcgtagg ggtacccctc gggggtatgc caattctttg ctcttcctaa
     3661 aggtggttcg cctttttcgg ttggcaaaaa agcatcaaca tccggaagtt caaggggtaa
     3721 cttctcttcg ggcaaggggt aaggtaatcc atccttatag taaattggga aaggctcgcc
     3781 ccagtaacgt tgacggctaa aaatggcatc gcgcaagcgg tagttcacct tgcttttacc
     3841 caacccccgc tgctcaatga tttcgcatat tcgtgtaatg gcttgtttga cttcaagccc
     3901 atcaatcagg gggctgttca ccacgatgcc atccttcgcg tcgtatgatt cctcccatgt
     3961 ttccggattg gtaggttgtt cgcccttcct aacaactgtt tgaataatag ggaggttgaa
     4021 agttcgggca aaggcgaagt cgcggctgtc atgggcagga acagccatga tggcgcctgt
     4081 tccatatccc gccagcacgt agtcgctaac ccaaatggga atgggctggt tggtaaaggg
     4141 attcaccgca tagcttccgg tgaactggcc ggtaattttt ttggcctcgg ccacccgctc
     4201 acgttcagtt ttgcgtttca cctcgttgca gtatgcctca acattatcgg catactcggg
     4261 tttggtaaga ccatccagca gctccgactc tggagcaagc accatgaagg ttgccccata
     4321 gatggtgtcg ggacgggtgg taaaaatttt aatttgctgc cggctatcgg ccacgctgaa
     4381 aagcacttct gccccttccg agcggccaat ccagtttcgc tgtatttcct taagcgagtc
     4441 tgtccagtcc agctgctcta aatcggaaag cagccgttcg gcgtaagccg aaatgcgaag
     4501 gcaccattgc ctcatcttac gctgctcaac cgggtgacca cctcggatag aaacgccctc
     4561 cttcacctca tcgttggcca gaacggttcc cagtgctggg caccagttta ccataacatc
     4621 ggcaaggtat gccagtcggt aagacagaag aagttcctgc tgctgcctgc catccatatt
     4681 cctccaatct gaagcagtaa aaattttggt ttcgctacaa gcagcatcaa catgctgatt
     4741 gccgtttcgc tcaaactcgg ctaccagctc atcaattggt tcggccttct gtgttttgtt
     4801 gttgtaccag tgcttaaaca tttgaataaa tgcccactga gtccatttgt agtatgatgg
     4861 ctcgcaggtg cgaaactccc ttgaccagtc gtagctgaac ccaattttat cgagctggga
     4921 gcggtagcgc tggatgttgt tctcggtagt aatggcggga tgctgtcctg tttgaatggc
     4981 atattgctca gcaggaagtc caaatgcatc gtaccccatg ggatggagca cgttgaaacc
     5041 ctgtaggcgt ttatagcgtg agtaaatgtc ggaagcaatg tagccaaggg gatgacccac
     5101 atgcaaacca gctcctgaag ggtagggaaa catgtcgagc acataaaatt ttggcctgtt
     5161 ggggttaaca tctaccctat aggtattcca ctgctgccaa agcgcctgcc acttctcctc
     5221 aattgcctta aagttataat ccatagaaaa ccagattggt tgggctaaat ttagctagca
     5281 aagataacta aaatgagcat ttcgcttaac attcaatgcc ggttttatta tgatgtacag
     5341 gagaatgccg acatgtcgcc ttcgcacctt cgcatctccc cggatgaaaa atacccaacc
     5401 gcaaaggtgc tgaagtgctt ttccaaacaa gggaaatttt atcctggcgt cttccttggg
     5461 gaaaaaatat tttacggggc tgctaaggac aaggctcaac aggactactc ccccacctcc
     5521 ttcaaaattt tctcacccag tccaatgcta tacccctcgg agaaaaacgt tatctccccc
     5581 ttgccattca ccaccgctac cacagggtaa tcggttgaaa ggggtttccc acaagcaata
     5641 gcaagcgcat tagccaaact ttgctgggaa tcatatccaa tagtaacgcc aatgggcaag
     5701 ggcaccccct ctaaaagctt ttgagcgttg aagccagatg gtagcttatc cttagcaata
     5761 gctagtacaa cagcaccgcc ccatttgtcg agctgcgttt tgatggccgc aaggtcattt
     5821 accaaatgtc tggtaggttc cttcattgga tctataatgg caactaccgt tgaccctcct
     5881 cctccaatct cgcccagatt cttgatatag cttccttcaa tatcgggaat aagcgggtcg
     5941 cctaaatttc caaggacaac cgttttacct tttgacgatg ggaattccag cgttaccaag
     6001 gttttttcat ctggataaat gctgaaatac tttaccctac agctcaccgc tccgttttga
     6061 tctctagtgc cgcaaaccaa cctgtaaagt ccttccctta gtgataggct aaaaggcagg
     6121 gtactaagct gggcatcgtt ctcaaaatca agggtaacaa acctgttgtt ttcaaatcgg
     6181 gctatggtgt aatgcttata gtacgacggt gaagcaatct ccatgccgga ggtagcagaa
     6241 agtgtaagtg ttccccagcc ggaagcttca acttccctct caccaaaatc aacatcatgc
     6301 cagctgccgc gcatgaggta ctgcggacgt ttggtagaag gttcaagccg cgcaggaatg
     6361 ccaaaactgc ggcaagccgc tacaaagagg atatcgcggg agtaggcatc ggctatgcct
     6421 aacctaacta caccctcggg gctaattgga accttatagt agttttcaac cgaatcaatt
     6481 ttgatgttct ctgaaatcca cttgaccagg ttggttggat cgttccggaa aaaacctatc
     6541 tggtcgaagg tgaaatagcc ctgcaaaaac tcccgccagg gtgtgatgaa ctcccttcca
     6601 accctggggg aaagaatgta ccggtcaaat aaattgaagc tacggctatc gagcaatgag
     6661 gggaaggagt caacagtttt aagatggctt cttaaagtag cagcggtaat gtcgtgcaag
     6721 tccttctccg ccagaattcc aagtagagcc atgcccacag gtgtttcacc cggctccatg
     6781 ttggtaataa aattttgaat ttccggccag ttcccccgac ttttagagag gaaatgccat
     6841 gccttttcgg cgttaatacc catacttgct gcaaacttaa cagctgacag ggaatcaata
     6901 aaagtagcaa tgtagcatcc tcgaaggcta tcctcaacag ctagccggcg gctgtttgca
     6961 gcaaccttat catcggcagc agacgtaacc gaaatttcgt cgggtggaac cagcaacagg
     7021 tctgtagctt ctgcggaagt cgttgaggag agcttcaact ccatttcgtt cagacttcta
     7081 tcaaccttga caaatgcggt tttcccatcc ctatgcgcat aaaccatcat atcgcccaat
     7141 cccgtagtaa tgctacacag gccattttta tcggtaacct tagaggcaat agggtaaaac
     7201 tcggcatagt tgtatatttg aaactcaacc ctagcggcct caaccggttg attagcttcg
     7261 tcaaccacag taacggaaag gtttttggat ggggcatatc gtggtaacag gtttatctcg
     7321 gcgaagcaat ccgattcggt aagtttttcc tcttggccat tatacttacc aaaaacaaag
     7381 gttcggatca tcattgcacg tttggctggt gctgcaaacc accccatatc gagttcgggt
     7441 tcgggttcgc aggctcccag gaaatgccag ctgccatcaa cccatacctc tacccacgca
     7501 tgattatcgt cggtatgtgc ccatcgtgga gtgtaaacct gacgggcggg aatacccacc
     7561 gaacggtaag ctgcaacagt aaaggttgat tcctcgccac acctgccaaa ggcagttctg
     7621 acggttgtta gcgggccgct ggtgcgctca tctgttggct ggtaggtaac cttttcatgg
     7681 caccagtggt ttacctccag ggcagcttcg tatgcggata acccggatac gaagctgccc
     7741 tggaggtaaa ccactggtgc catgaaaagg ttacctacag ggcagct
//
LOCUS       DAOPHX010000115         7754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_11970_length_7754_cov_5.561631, whole genome shotgun sequence.
ACCESSION   DAOPHX010000115 DAOPHX010000000
VERSION     DAOPHX010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7754
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11970_length_7754_cov_5.561631"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PK502_08565"
     CDS             <1..819
                     /locus_tag="PK502_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPH00908.1"
                     /translation="ISVESAFGFLSNSSALIANAGHNLSDTLALAFSWIAVVISHRKP
                     TKRFTYGFRRSTILVAMLNTILLLAVVVFITIEAIDRLRNPSEINTRSVIVIAAVGIL
                     VNGLTAWLFIKDKKKDLNIRSTFVHFVADALLSLGVVAAGVMITFTGFVWIDPLVSLV
                     IVAIILLSAIRLLVDSVSLALDAVPEGINIQAVSNYLKQLPEVKGIHDLHIWALSTTD
                     VALSVHLCTSKQTDLNFISTIQQQLQKQFNIEHSTIQVEYGVTDSECTSSCHPN"
     gene            complement(842..1084)
                     /locus_tag="PK502_08570"
                     /pseudo
     CDS             complement(842..1084)
                     /locus_tag="PK502_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616585.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
     gene            complement(1292..1876)
                     /locus_tag="PK502_08575"
     CDS             complement(1292..1876)
                     /locus_tag="PK502_08575"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPH00909.1"
                     /translation="MESLKNRILNCTKCDLYKTRNNPIFGEGNIHAPIFIIGEAPGRE
                     EDLVGRPFIGKSGQLLDKILAACNFNRNYHVFISNIVRCRPPENRVPTSREAEICMPY
                     LLEQIELVNPKILVLLGSTALKYMAGADHRITKERGTWLEVYGRLAMPVYHPAALLRD
                     PSLKRDTWEDFKKIFYKYRELVNPEHQSPHISIG"
     gene            complement(1958..2746)
                     /locus_tag="PK502_08580"
     CDS             complement(1958..2746)
                     /locus_tag="PK502_08580"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPH00910.1"
                     /translation="MNPIEKIELDGVKNIVVVASGKGGVGKSTVSSNLAISLARNGYK
                     TALVDADIFGPSIPRMFGIEKAQPEVTHLGDKEVMFPIEKYGVKIMSIGFFIKKNQGL
                     IWRGPMAANAIMQLFENTLWNDIDYMIIDFPPGTSDIQLTTVQKLNVTGAIIVTTPQE
                     VALNDARKAASMFNNPDLNVPIWGVIENMAWFTPQPHPDEKYYIFGQGGGEMLAKELN
                     TKLLGQIPLVAEVGDAAEKGLSVYNQSNPTVIEAFEKLTETITA"
     gene            complement(2752..4107)
                     /locus_tag="PK502_08585"
     CDS             complement(2752..4107)
                     /locus_tag="PK502_08585"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPH00911.1"
                     /translation="MKRTDVLVIGGSAAGMVTATTGKAHWPEKSFTLVKKQKEVMVPC
                     GIPYIFGTLESSEKNIMPVDSMMEKIGVKSLVDEATDIDTKGKVVSFASGETIQYEKL
                     VIATGSNPVKPKWLAGADLENVFVIPKDRVYLDEMKKKLGSVKNIAVIGAGFIGVELS
                     DELVKCGLNVTLIEKLPHILNLAFDVELSSKIHEMLTARGVKVITGVGISKVHGTGKV
                     QSIELEDGRKMEMDAVVLSMGYRPNTELAKKAGIFIDEDNFIAVDEYMRTHTPDVFAV
                     GDCAQKRDFVTRRRVATMLASTACAEARIAGMNLFNLSVVKTFSGTIAMYSTAIGETG
                     FGTAGVTEQRAQQEGLSTLTGIFEGVNRHPGNLPGTHKQIVKLVAASNSGVIVGGEVI
                     GGLEAGELTNVIGLAIQNRMSVNSFLTMQIGTHPCLTASPAAYPLIKAAEQIAGKMIK
                     K"
     gene            complement(4126..4656)
                     /gene="cobO"
                     /locus_tag="PK502_08590"
     CDS             complement(4126..4656)
                     /gene="cobO"
                     /locus_tag="PK502_08590"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPH00912.1"
                     /translation="MNQSNQQNGYIHLYTGNGKGKTTAAIGLAVRAAGANKTIFIGQF
                     VKGMHYAELVSLQRIPQIKVKQFGLDCFIVNNPTQKDVDAARAGFDEISEIVLNNSYN
                     MVILDEICIAIYYRLIGVNEVIDVLGKKPREMEVVLTGRYAPQELIDIADLVTEMKEI
                     KHYYNQGIEARKGIEY"
     gene            complement(4714..5403)
                     /locus_tag="PK502_08595"
     CDS             complement(4714..5403)
                     /locus_tag="PK502_08595"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HPH00913.1"
                     /translation="MIKGIQKSKLGIITDIILLVFLALMAGIGFLMKYVLLPGIQRNS
                     KYGADINLEFLGMDRHQWGTIHLITSKIFITLIILHIILHWDMIICILDKMIPRKLVR
                     SWFLAILAIITLTQFINPFLASPALVSHENNYRNRAERIESEPNGMDPLHKTANVEAK
                     KPIPDQSKTSRHSRAEEEYGVHGIQTINGVSVQFTIPAGFIGKELKISEGNQYQPLVV
                     VEKGMDLLCRR"
     gene            complement(5425..6366)
                     /locus_tag="PK502_08600"
     CDS             complement(5425..6366)
                     /locus_tag="PK502_08600"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPH00914.1"
                     /translation="MDELVFGPVPSRRLGKSIGVNNIPHKVCSYSCSYCQVGKGEKIQ
                     IERQEFYNPDTLVKQVEQKLNMTSHAEYPDYITVVPDGEPTLDINLGKLITKLKTFGL
                     PVAVITNSSLIYLDDVKRDLSNADYISFKVDAVNHSIWKYINKPHRKLDLHEILNSLM
                     DFSKYYRGKYVTETMLVKGDNTADIELAATAQFLQQLKPSIAYLSIPTRPPAFENTFP
                     PCEETLLKAYEVFYWHCLNVEYLTGYEGNAFTSSGNFENDLLGITAVHPMRIDAVKEL
                     MAKTKTNDGVLEKLVEENKVKRILYERQTFIVRSFNK"
     gene            complement(6384..6722)
                     /locus_tag="PK502_08605"
     CDS             complement(6384..6722)
                     /locus_tag="PK502_08605"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPH00915.1"
                     /translation="MRIAFTSTGKSWDSIIDSRFGRTEYIVIFDEETKTLELVDNSAV
                     KNEAHGAGTATAQKMYEVKPDVLITGNGPGETAATALKRLNMKIYVDAHNLTLEQAYE
                     SYRNGALKEI"
     gene            complement(6728..7606)
                     /locus_tag="PK502_08610"
     CDS             complement(6728..7606)
                     /locus_tag="PK502_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPH00916.1"
                     /translation="MKEIVVLSGKGGTGKTSITASLAVLAGSEAVVADCDVDAANMHL
                     LMQPDFALSSEFYSGELAEINQNICTQCGLCSNVCRFDAIPFQNNKYTVNPLACEGCG
                     YCQKVCPTEAISMKKRKSGYVYISNTKVNSTMVHARLDIGAENSGKLVAKVKSKAKEL
                     AEHQNKYFVIVDGSPGIGCPVVSSLSGASYVVLVTEPTVSGLHDLERIYSVIKRFRIK
                     AGCIINKYDINPKKSEEIIEFLNRENIVHLVSIPYDIAFTEAMTQAKTIVETSSRISQ
                     KLIDAWNKIKEEAAKN"
BASE COUNT         1941 a         1713 c         1632 g         2468 t
ORIGIN      
        1 atttccgtag aatctgcatt tggttttctt tccaattcat cggcgcttat agctaatgcc
       61 gggcataacc taagcgacac gcttgccctg gcattttcct ggatagcagt tgttatttca
      121 caccgaaaac ctacaaaacg gtttacctac ggttttcgac gctcaacgat tttggtggcc
      181 atgctaaaca caattttact tttggccgta gtagtgttta ttacaattga ggcgattgac
      241 aggctgcgaa acccctcgga aataaacacg aggagtgtta tagtaattgc tgctgttggg
      301 atactggtca atggcctaac agcctggctt tttataaagg acaaaaagaa agatttaaat
      361 attcgaagca cctttgttca ctttgttgcc gatgccttgt tatcgcttgg agttgttgct
      421 gcaggggtta tgataacttt tacaggtttt gtttggattg atccgcttgt ctccctggtt
      481 attgtcgcca taattcttct tagcgccata aggctgctgg ttgattcggt ttcgctagca
      541 ctcgacgcag tacctgaagg tattaacata caggctgtta gcaactactt gaagcagctt
      601 ccagaggtga agggcataca cgacctgcat atctgggcgc taagcaccac agatgtagca
      661 ctttcagttc acctttgcac cagcaagcaa actgatttaa acttcatatc aacaatccag
      721 caacagctgc aaaagcagtt caacattgag cattccacca ttcaggtaga atatggggta
      781 accgattcag aatgcacatc atcatgccat cctaactagg aaaactatgg cgtaacccag
      841 cttatgccat cctgcgaccc gccgtgtagc ttctccaacg atcaaccagc tgggccatat
      901 cgttaggaag tggcgattca aagttcatct ccatacccga tagggggtgt gtaaatccca
      961 gtgtttttgc gtgcagtgcg tggcgtgggc atatctcaaa acagttttcc acaaattgtt
     1021 tgtagtggct atggagcgtg cccctaagaa tcttatttcc tccatacacc tcatcgttaa
     1081 aaatagcgtt cgggtattgt ttgccagata gcaggttttg tggctagtcg tattatattc
     1141 gatgttaaac aaggcgatac tgcggagcaa ggcgatgagt tggaatttat cgtgtttgta
     1201 tctagggtcg acctttacct gcctttgact ttcaagccaa caatcgcaca aacgataagg
     1261 ttaaagggaa tattccaatt attggctaat tttagccaat actaatgtgt ggtgattgat
     1321 gttcggggtt taccagctcg cggtacttat aaaaaatctt cttaaaatcc tcccaggtat
     1381 cgcgtttcag cgaggggtcg cggagtaatg cggccggatg gtaaactggc atggccaaac
     1441 ggccataaac ttccagccaa gtacctcttt ctttagtgat tctatgatct gcaccagcca
     1501 tgtatttaag tgccgtagag cctagcagta ctaatatttt ggggttaacc agttcaatct
     1561 gttccaacag gtatggcatg caaatttcgg cctcgcggga agttggtaca cggttttcgg
     1621 gtggcctaca cctaactatg ttgctgataa aaacatgata gttccggttg aagttgcatg
     1681 cagccagtat cttatcgagt aattgacctg atttcccaat aaatggtcga ccaactaaat
     1741 cttcctcgcg accgggagct tcgccaatta taaaaattgg agcgtgtata tttccctcgc
     1801 caaaaattgg attattcctt gttttgtaca aatcgcattt ggtgcaattt agtattctgt
     1861 tttttagcga ttccattgat aattggttgt aggagtgtat ttatccaaaa aaaaagaccc
     1921 ggaaagaata aatatcgggt ctgtttggat gattactcta cgctgtaata gtctcggtta
     1981 gcttttcaaa ggcctcaatt actgttgggt tgctttggtt gtaaacgcta agcccctttt
     2041 cagcagcgtc accaacctcc gctactaagg gtatttgccc tagcaacttt gtgttgagtt
     2101 cttttgccag catttcgcca ccaccctgac caaatatata gtatttctca tcggggtggg
     2161 gttgaggagt aaaccaggcc atattctcaa ttacacccca tattggaaca ttcaaatcag
     2221 gattattgaa catcgatgct gctttgcgag catcgtttaa tgctacctcc tgaggtgtag
     2281 tgacaataat agcccctgta acattgagtt tttgaaccgt ggtgagttgt atatcgcttg
     2341 ttcctggagg aaagtcgata atcatataat cgatatcatt ccaaagggta ttctcgaaaa
     2401 gttgcataat tgcattggca gccatagggc cacgccagat taagccttgg tttttcttaa
     2461 taaaaaatcc gatggacata atttttacac catacttttc aattgggaac atcacctcct
     2521 tatcgcccag gtgcgttacc tccggttgcg ctttttcaat gccaaacatt cttggaattg
     2581 atggcccaaa aatatcggca tcaacaaggg ctgttttgta accgtttcgg gcaaggctta
     2641 tggccaggtt cgacgatacg gttgattttc caacaccccc tttccccgag gcaacaacca
     2701 cgatgttctt tacgccatct agctcaattt tttcgatagg attcataagg gctatttttt
     2761 aatcatttta ccagctattt gttctgctgc tttaataagt ggataggcgg ctggcgatgc
     2821 ggttaggcat gggtgagttc caatttgcat ggttaggaac gagttaaccg acatgcggtt
     2881 ttggattgcc aatccaataa catttgtaag ctcgccagct tcaagaccac caataacctc
     2941 gccacctaca attactccag aatttgatgc ggcaaccagc tttacaattt gcttatgcgt
     3001 tccaggaaga ttgccggggt gcctgttaac tccttcaaaa attccggtta gagtagataa
     3061 tccttcttgt tgagcgcgtt gctctgtaac acctgctgtt ccaaaccctg tttcgcctat
     3121 tgcagtggaa tacattgcta tggtaccgct gaaagttttt actacggata ggttgaatag
     3181 gttcatcccg gcaatacgtg cttcggcgca agcggtagat gccagcatgg ttgccacacg
     3241 gcggcgggta acaaaatcac gcttttgagc gcagtcccct actgcaaata catcaggagt
     3301 gtgagtgcgc atatactcat caacagcaat gaagttgtcc tcatcaataa aaattccggc
     3361 ctttttagct agttcggtat tgggcctgta acccattgat agaactacag catccatctc
     3421 cattttccgt ccatcctcta gctcaatgga ttgaactttg ccagtgccat gaaccttgct
     3481 gatgcctact ccggtaataa ccttaactcc gcgagcggtt aacatctcat gaattttgct
     3541 ggagagctcc acatcgaatg ccaggtttag tatatgcggg agtttttcga taagggttac
     3601 gttaagaccg cacttcacca gctcatcgga aagttcaacg ccaataaagc cagcaccaat
     3661 aacagcaatg ttttttacgg agcccagctt ttttttcatt tcatccaggt aaaccctgtc
     3721 ctttggaatc acaaaaacat tctcaaggtc ggcaccggca agccatttag gcttaacagg
     3781 attggaaccg gttgcaataa ccagtttctc gtactggatt gtttcgccgc tggcaaagct
     3841 caccactttc cctttggtat cgatatcggt agcttcgtca accaatgact taactccaat
     3901 tttctccatc atgctatcaa cgggcatgat gtttttctcg ctactctcca gagtaccaaa
     3961 gatgtaaggg attccgcagg gaaccataac ttctttctgt ttcttcacta aggtgaaact
     4021 tttttcaggc caatgggctt taccggttgt tgcggttacc attccggcag cgctacctcc
     4081 aattactaat acatctgttc gtttcatagt ttatttattt tgtggttagt attcaatacc
     4141 ctttcgggct tcaattcctt ggttgtagta atgttttatt tccttcattt cggttacaag
     4201 atcggcaatg tcaataagtt cttggggcgc ataccttccg gtaagaacaa cctccatttc
     4261 gcgtggtttt ttgccaagca cgtctataac ctcgttaaca cctattagcc tgtagtatat
     4321 ggcgatgcaa atttcgtcaa ggatcaccat gttgtagcta ttgttcagca ctatttccga
     4381 aatttcatcg aaccctgctc ttgcagcatc aacgtctttt tgcgtggggt tgtttacaat
     4441 aaagcagtcg agtccaaatt gcttcacttt aatttggggg atgcgttgga gcgatactag
     4501 ctcggcataa tgcatgcctt taacgaattg gccaataaat attgttttgt tagcaccagc
     4561 ggctctaacg gcaagcccaa tggctgcagt tgtttttccc tttccatttc ccgtataaag
     4621 gtggatgtag ccgttttgtt gattactttg gttcatggtt taagtgcttt attgtgccgc
     4681 cagagaattc ttgtattttt gcaatggcat tgcctacctc cgacatagta aatccatacc
     4741 cttttctaca acaaccagag gttgatactg gttgccctct gatattttaa gttccttgcc
     4801 gataaaacct gctggtatgg taaattgtac cgataccccg tttattgttt ggatgccgtg
     4861 tacaccatac tcctcctcgg ctctagagtg cctggaagtt ttgctttggt cgggtatcgg
     4921 tttctttgct tctacgtttg ctgttttgtg aagcggatcc attccatttg gttcgctttc
     4981 aatcctttcg gctctgttcc ggtagttgtt ctcgtggcta accagagccg ggcttgcaag
     5041 gaatgggttt ataaattgtg ttagggtaat tattgccaaa attgctaaaa accagcttct
     5101 tactagtttt cgcggtatca ttttatcaag aatacaaata atcatatccc agtgcaggat
     5161 gatgtgcagt ataatcaggg ttataaagat tttgcttgta attaggtgaa tagttcccca
     5221 ttgatgccta tccataccaa ggaattcaag gttgatatct gctccgtact tgctgtttcg
     5281 ctgaatgcca ggaagcaaaa catacttcat gaggaaacca atgcctgcca ttagagctag
     5341 aaataccaaa agtataatgt cggttatgat accaagcttt gacttttgta ttccttttat
     5401 catggcgttt atattttttg aaatctactt attgaagcta cgaacaataa aagtttgcct
     5461 ttcgtagagt atccttttaa ctttattttc ttctaccagc ttttcaagaa caccatcgtt
     5521 tgttttagtt ttagccatca gctctttaac tgcatcaatc cgcatggggt gaaccgcagt
     5581 aatacccaac aggtcgttct cgaagtttcc cgacgaggta aacgcgttcc cctcgtatcc
     5641 ggtaaggtac tcaacattca ggcaatgcca gtagaaaact tcgtaggctt taagtagagt
     5701 ttcttcgcaa ggagggaatg tattttcaaa agccggtggt cgagtgggaa ttgaaaggta
     5761 tgcaatactc ggtttaagtt gttgcaagaa ttgtgcggtg gctgccaact ctatatcggc
     5821 agtgttatct ccttttacca gcatggtttc tgtaacatac ttccccctat agtatttaga
     5881 aaaatccatt aaggagttta gtatctcgtg tagatctagc ttacggtgtg gtttattaat
     5941 atacttccag atgctgtgat ttactgcatc aaccttaaat gatatgtaat cggcgttcga
     6001 taaatctctt tttacatcat ccaaataaat aagggaggag ttggtaataa cagcaacagg
     6061 caacccaaaa gtttttaatt tggtgattag cttgccaagg ttaatatcca gcgtaggctc
     6121 tccgtcgggc acaactgtta tgtagtcggg gtactcggca tggctggtca tgttgagttt
     6181 ttgttccacc tgctttacaa gagtgtccgg attgtaaaac tcctgacgct ctatctgaat
     6241 tttttcccct ttaccaacct ggcagtacga gcatgagtag gagcaaacct tatgagggat
     6301 attgtttact ccaatgcttt tccctaacct acgtgaagga acggggccaa atactaactc
     6361 gtccattgtt ttgaattttt atcctagatt tccttcaatg caccattcct atagctttcg
     6421 taagcttgtt ccagtgttag gttatgggca tctacatata ttttcatatt aaggcgtttt
     6481 agtgcggttg cagcagtttc gccagggcca ttacctgtaa taaggacatc gggttttacc
     6541 tcgtacattt tttgggcagt ggctgtaccg gctccatggg cttcgttctt tacagcgcta
     6601 ttgtcaacta gctcaagcgt ttttgtttcc tcgtcgaaga ttacgatgta ctctgttctc
     6661 ccaaagcggg aatcaatgat agaatcccag cttttccctg ttgatgtaaa tgcaattctc
     6721 atggtactta gtttttagct gcttcttctt ttattttgtt ccaagcatct ataagctttt
     6781 ggcttatgcg gctgcttgtt tccactattg ttttggcttg tgtcatagcc tcggtaaatg
     6841 caatgtcgta tggaatgctg acgaggtgga caatgttttc cctattgaga aactctataa
     6901 tttcttcaga tttttttggg ttgatgtcgt atttgttgat tatgcatcct gcttttatac
     6961 ggaaccgctt tataacactg tatatccgct ccaaatcgtg caatccggaa acggtaggct
     7021 cagtaaccag caccacgtag cttgcgcctg ataacgacga caccactgga cagccaattc
     7081 cgggcgatcc atcaacaata acgaagtatt tattttgatg ctccgccaat tccttagctt
     7141 tgctttttac tttagcgacc aatttccccg agttctcggc gccaatatcc aaccgggcat
     7201 gcaccattgt gctgtttacc ttggtgttgg agatgtaaac gtaccccgat tttctttttt
     7261 tcatggatat tgcttcggtt gggcaaacct tttggcagta cccacagccc tcgcacgcta
     7321 aagggtttac agtgtatttg ttattttgaa atggaatggc atcaaaacgg catacattgg
     7381 aacacagacc gcattgagtg caaatatttt ggttgatttc ggctagttct ccgctgtaaa
     7441 actcgctcga tagggcaaaa tcgggttgca tgagtaggtg catgttggca gcatccacat
     7501 cgcaatcggc cacaaccgcc tcactacctg caagcacagc cagcgatgct gtaattgagg
     7561 ttttacctgt tcctcctttg cccgatagta ctactatttc tttcatttag actctagatt
     7621 ttagacacaa gacattagtt taattgattt tgctgatgta actgacaaat tcatctagag
     7681 attttttcat cgattcaatc ttgttgtaaa ccagctctcc cttcgagtag cagcttgcaa
     7741 tatttttatc gaat
//
LOCUS       DAOPHX010000116         7596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_12340_length_7596_cov_5.677364, whole genome shotgun sequence.
ACCESSION   DAOPHX010000116 DAOPHX010000000
VERSION     DAOPHX010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12340_length_7596_cov_5.677364"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="PK502_08615"
     CDS             complement(<1..1409)
                     /locus_tag="PK502_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPH00917.1"
                     /translation="MSSNFFKTKPIQKILGEAGDGEHGFKKTLGPMNLTTLGIGAIIG
                     AGIFVLTGQAAAQYAGPAIVISFIISGLACAFAGLCYAEFSSMIPISGSAYTYAYATL
                     GEFLAWVIGWDLILEYLFAASTVSVGWSGYIVSLLYQIGVNIPPEFTAARGMELLQVP
                     ANIPGIDAGWLPKTGELVEFLANQGVNASSLPMVTSIINIPAMFIIAVISMLLIVGIK
                     QSANFNNIMVIIKVSVIVLFIALGFAFIKSSNLHPFVPERVGEWGHFGWGGILRGAAV
                     IFFAYIGFDAVSTAAQEAKNPQRDMPIGILGSLVISTVLYILVAIVLTGMVNYKFLNV
                     PAPIALAVDAMGEQYSWLRIIIKIAALAGLTSVILVMLLGQPRIFFTMAKDGLLPPVF
                     AKVHPKYRTPYLSTIITGAVAMILAGVLPISILGELVSIGTLLAFVIVCIGIIVLRKK
                     RPDLPRPFKTPWVPLVPILG"
     gene            complement(1979..4285)
                     /locus_tag="PK502_08620"
     CDS             complement(1979..4285)
                     /locus_tag="PK502_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPH00918.1"
                     /translation="MKRKLKIRQKILLYILGATGLFYIACVGYILITSRQTIYKEAME
                     KTQLTAEVSAKEVTAIFERELSLVRTLSQALTVYQELPQEQWHKLFLDMYLPVLRENP
                     QIYSLWDSWEYKYYIPGYTNDFGRFVITTWREGSQLKWLYDERSMEGDPPIYGGFKRN
                     GVEAIWEPYVDQVTKGKAEVKLMITYASPVYVNSEFAGAIATDVGLDNLQEMVSKIKP
                     VTGSYAYLVSNSGIIAGHPNKEFIFKKIADAYPSDEKKYQLTERIAKGEKFNYTIKDS
                     SGREHLICYSPIVAGKVKTPWALVLSTPVDVIMAQARHTLWVSIIVSLLGLIAITLVL
                     LFVSGNLTRPIKKITEALKRLARGEISEELMVSINSGDEIEEMAVAFNTSLSGLHHKT
                     LFANDIGKGNYATQLELLSEADVLGKSLITMRDSLKNAAKEEENRKLEDQKRSWANEG
                     HAHFGEILRQNNNDLQKLCDNVIISLVKYVGANQGGIFLLNDDDKMHPFFELISAYAW
                     DRKKYMTKQIELGEGLVGACAMEKETIFLTEVPDDYVNITSGLGEANPRCVILVPLKH
                     EDEVVGVVEMASFKVLEKHEVEFIEKVAESIASTILSVKINARTKMLLEQSQMQAEEM
                     KAQEEEMRQNMEELQATQEEVSRKSGEIEEFMNSINSTAYVVEYDLGGNVINVNDSYI
                     RLVGVPREQIIGSHHTQNLILNDKQKKEYGTFWENLKAGVSKKLRTTITSGGRSIELL
                     ETYIPVRDVDGKVFKVMKLAFEVNEFQQ"
     gene            4444..7332
                     /locus_tag="PK502_08625"
     CDS             4444..7332
                     /locus_tag="PK502_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPH00919.1"
                     /translation="MTLCSALVTFSMLGMAQDYKYQSVPGDPLNARIYTLANGLKVFM
                     SVSKDEPRIQTYIAVRVGSKNDPKETTGLAHYFEHMMFKGTPQFGTANWESEKVLIAK
                     IDSLFEVYRTKTDSLERARIYHVIDSISYEASKLSIPNEYDKLMTAIGSIGTNAGTSN
                     DYTIYMENIPSNQLENWAKIQAVRFANPVLRLFHTELETVYEEKNMSLTNDGRKASET
                     LLKGLFPNHPYGQQTTLGEAEHLKNPSMKNIREFYAKYYVPNNMAVVMAGDFDPDEAI
                     RIIDSNFGSLKPGNVPSLVYQPEVPFTQPKEFEVVGLEAENVIIGYRFRGANSPDNDM
                     LDLISAILYNGRAGLIDLNINKKQLTLGCQAYNRAMADYSILQFSGRNKKGQSLEEVK
                     NLLLQQVELLKKGDFPDWMLEAAVNNVKLRQMSQLESARGRASLMYSAFLNNIEWADE
                     VAYISRLEKITKEQVVRFANDNLGSNYVVVYKQQGKPEEVAKVAKPPITPIYINRDAE
                     SDFLKMIKNSTVKPIEPRFIDYDKDITKFKLNTNVEVLYTKNAENSTFNLTLYYPFGR
                     LANPLLTHAADYIQLLGTSKLTAEQLSQEFYKLACNFNISVRDEEMLISLTGLSDNAD
                     KAFELMENLLADCQPNSDALSMYITNQLKSRDDAKKRQQSVFSALVSYATYGEENPQR
                     LILSNEELKGLAAQPLVDLIREFTSYPHIILYYGPQDVKGRKKMLTEKHIQPKKYKSL
                     PVNKKFEETSTTTERVLFVNYDAKQSYLQTISKGELYSDNLYPVVTLYNEYFGGGMNA
                     IVFQEMREKRGLAYTARSLYSIPDEPNRVFMNTSFIATQNDKVIDAFNAFNELFNNMP
                     EAEIGFNLAKESLISNIRSQRIIRMALLYNYLDARRMGWNRDMRQTLFEVIPGMTLND
                     VRLFQQTHVKDKPKTYVILGKESDMDFNRLSSLFGPVTKLSLEDIFGY"
BASE COUNT         2094 a         1787 c         1678 g         2037 t
ORIGIN      
        1 cccagaattg gcacaagcgg aacccagggg gttttgaacg ggcggggaag atcgggacgt
       61 tttttcctca gcacgatgat gccgatgcag acgataacaa acgccaaaag ggtaccaatt
      121 gaaacaagct cgcccagaat gctgatgggt aaaacgcctg caagtatcat tgccactgcg
      181 ccggttatga tggtgctaag gtagggtgtg cggtattttg gatgaacctt ggcgaataca
      241 ggtggaagca atccatcctt tgccatggtg aagaatattc ttggctggcc aagaagcatg
      301 accagaataa ccgaggttaa tccggccagc gctgcaatct taatgattat acggagccag
      361 ctgtactgtt cacccattgc atcaaccgca agagctatag gagcaggaac atttaagaac
      421 ttgtagttga ccatgcctgt taatacaata gccactaaga tgtagagcac ggttgagata
      481 accagcgaac ccagaatacc tatcggcatg tcgcgttggg gatttttagc ctcctgggca
      541 gccgttgaca ccgcatcgaa cccgatgtag gcaaaaaaga ttaccgctgc accccttaga
      601 atgccgcccc agccaaagtg cccccactca ccaacccttt cgggcacaaa tggatgcaga
      661 tttgaacttt taataaaggc aaagcccagc gctatgaaga gaacaataac cgatacctta
      721 atgataacca tgatgttgtt gaagttggcc gattgcttga tgcccactat cagcagcatg
      781 cttattacgg ctatgatgaa catggccgga atattgataa tagaagttac catgggcagc
      841 gagctggcat taaccccctg gtttgccagg aactcaacaa gctccccggt ctttggaagc
      901 catcccgcat caatacccgg tatatttgca ggaacctgca gcagctccat ccccctggct
      961 gccgtgaact cagggggtat gttcacccca atttggtata ggaggctgac aatatacccc
     1021 gaccacccta ccgacacggt tgaagcggca aaaaggtact cgaggattag gtcccatcca
     1081 attacccatg ccaaaaactc acccagggtt gcataggcat aagtgtaagc gcttcccgag
     1141 attggaatca tggaggaaaa ctcggcgtag cataaacccg caaatgcaca ggccaaaccc
     1201 gatatgatga acgagattac gatggccggg cctgcatact gggcagccgc ctgacccgtt
     1261 agcacaaaaa ttcctgcacc aattatggca cctatgccta gagtggtgag attcatcggg
     1321 ccaagggttt tcttaaaccc atgttcgccg tctccagctt cgccaagtat cttttgaatg
     1381 ggtttggttt taaaaaagtt agagctcatc ctcttaatgt tttaaataaa acctataatg
     1441 cccttctttc gtgctattgg cacgattggc aggccatcct gctatggcta tttattgctg
     1501 ttgtatggta gattgtttta gcttaggcaa atgttgtaaa aaaaatcata cgttgaaaaa
     1561 aaaatgcgga atatgtaaag ctaaacctga agatatttag aaaagtctag tttaattagg
     1621 ttacaagccc acacccgtgg aaaaatcaac aagtgttttt tcaaatttta aacacgcttt
     1681 ccagctattg aaaatgatca aagttttgta tatccccctg ctagctgttg cgtctggttt
     1741 actaatcgta gtgttacaga aacttgttat tcagcatgca cctatcgctt gtttaaaggg
     1801 ttacctataa atgatggagc tagcctggca tacaacatgg caacctaaaa ataaaaatac
     1861 cgccgctgga aatagtagca taaccaacgg cggttttatc ctggggcatt cttaatgcgc
     1921 taacctgctt gcaaactcgg cagcatgtaa ataagcctgt gaagcagggt tcattttttt
     1981 actgctgaaa ctcgttaact tcaaaggcca gcttcatcac cttgaaaact ttaccatcaa
     2041 catctcttac aggaatgtag gtttcgagta gttcaatact tcggccacca gacgttatgg
     2101 ttgttcgcaa cttcttactt acgcccgcct tcaggttttc ccagaaagtc ccatactcct
     2161 ttttctgctt atcgttcagt atcaggtttt gtgtatgatg tgaaccgatt atttgctccc
     2221 gggggacacc aacaagcctg atgtaggaat cgtttacgtt gataacattt ccgcccagat
     2281 cgtactctac aacataagcc gtgctattga tggagttcat aaactcttca atttcgcccg
     2341 atttacggct gacttcctcc tgtgtggctt gtagctcctc catgttctga cgcatttctt
     2401 cctcctgcgc cttcatctct tcggcttgca tttgcgactg ttccagcagc attttggtac
     2461 gtgcgtttat cttcaccgaa aggatggtgg aagcaatgct ttcggcaacc ttttctataa
     2521 actcaacctc atgcttctcg agcactttaa aggaggccat ttcaacaaca cctaccactt
     2581 catcctcatg tttaagcggt acaagaatga cacatcgagg gttagcctcg cctagcccgg
     2641 aggtaatgtt cacgtaatca tcgggaactt cggtaaggaa tatggtttcc ttttccatgg
     2701 cacaagcgcc aacaagtcct tctcccagct ctatctgctt ggtcatgtat ttttttctat
     2761 cccacgcata ggctgaaatg agctcgaaga atggatgcat cttatcatca tcgttgagca
     2821 ggaaaatgcc accctggtta gctccaacat acttaaccag cgagatgatg acgttgtcgc
     2881 acaatttttg caaatcgttg ttgttttgcc ggagtatttc cccaaaatgt gcatgtccct
     2941 cgttggccca cgagcgcttc tggtcctcca gcttcctgtt ctcttcctcc tttgcggcat
     3001 tttttaagct atcgcgcatg gttatcagcg atttgcccag cacatccgcc tcgctcagca
     3061 gctcgagctg tgtagcgtag ttcccttttc caatatcgtt ggcaaatagg gttttatggt
     3121 gaagtcctga cagtgaggtg ttgaaggcaa ctgccatttc ttcaatttca tcgcctgaat
     3181 taatgctaac catcagctct tccgaaattt ctccccgtgc aagccttttc aacgcttcgg
     3241 taatcttttt gattggtctg gtgagatttc cggatacaaa tagcagtaca agtgtaatgg
     3301 ctatcaggcc aagcaggcta acaattattg aaacccaaag ggtatgcctg gcctgtgcca
     3361 tgataacatc aacaggtgtt gaaagcacta gagcccaggg ggtttttacc tttcctgcca
     3421 caataggtga atagcaaatc aggtgttccc gacccgaaga atcctttatg gtatagttga
     3481 acttttctcc tttggcaatg cgctcggtaa gctggtattt tttttcatca gatggatatg
     3541 catctgctat tttcttgaaa atgaactcct tgttgggatg acctgcaatg attcctgagt
     3601 tggataccag gtatgcgtag ctaccagtaa ccggcttaat tttagaaacc atttcctgca
     3661 ggttatctag tccaacatcg gtggcaatag cacctgcaaa ctcactgttg acatagactg
     3721 gtgaagcgta ggttatcatc agtttaacct cggctttacc tttggtaacc tgatcaacgt
     3781 agggctccca gattgcctca acgccattac gcttaaaacc gccataaatt gggggatcgc
     3841 cttccatgct acgctcgtcg taaagccatt taagctggct gccctcgcgc catgtggtaa
     3901 ttacaaatcg tccaaaatca ttggtgtacc cgggaatgta gtacttatac tcccagctgt
     3961 cccaaagcga gtatatctgc ggattttccc tgagtacagg caggtacata tccagaaata
     4021 gcttgtgcca ctgctcctgt ggaagttcct ggtacaccgt taaggcttgc gagagggtgc
     4081 gaaccagcga tagctcacgt tcaaaaatgg ccgtaacttc cttggccgat acttctgcag
     4141 taagctgagt tttttccatc gcctccttgt agatggtttg ccgggaggtg attaggatat
     4201 acccgacaca ggcaatgtag aataaccctg ttgctcccaa aatgtaaagc agtattttct
     4261 gtcggatttt aagtttgcgc ttcatggctc tttaaaattt gctttaaact tataccattt
     4321 ttgaatacta tgcaacaaca tcaggaaatt taattgccta aacatgttaa acttaggtcg
     4381 gcggatttcg tcttatggga agtaaccaaa acccaactag aaatgacctg ttttttaaaa
     4441 aaaatgactt tatgctcagc gctggttacc ttttccatgc taggcatggc acaggattat
     4501 aaataccagt ctgttcctgg cgacccgcta aacgccagaa tttatacctt agctaatggt
     4561 cttaaggtat tcatgtcggt aagtaaggat gagccccgta ttcagaccta cattgccgtt
     4621 agggtgggaa gtaaaaacga ccctaaggaa acaacggggc ttgcccacta ttttgagcac
     4681 atgatgttta agggaacacc ccagtttggc acagccaact gggagagcga gaaggtactt
     4741 attgcaaaaa ttgactccct ttttgaggtg taccgaacta aaaccgatag cttggagcgt
     4801 gcacgtattt accatgtaat tgacagcatt tcctacgagg cgtcaaagct cagcattccc
     4861 aacgagtacg ataagcttat gacggcaata ggctcaattg gcactaatgc cggaacgtct
     4921 aacgattaca ctatctacat ggagaacata cccagcaacc agctcgagaa ctgggcaaaa
     4981 attcaggcag ttcgctttgc aaaccctgtt ttaagattat tccacaccga gcttgagaca
     5041 gtttatgagg aaaagaacat gtcgcttact aacgatggcc gaaaggcatc cgaaaccctc
     5101 ctcaaggggc ttttcccaaa ccatccctat gggcagcaga ccacccttgg cgaggcggaa
     5161 cacctcaaaa atccatcaat gaaaaacata cgcgagttct acgccaagta ctacgttccc
     5221 aacaacatgg ctgttgttat ggcgggtgat ttcgaccccg atgaggctat caggattatt
     5281 gacagcaatt ttggaagcct gaaacctgga aatgttcctt ccttggtgta tcaacccgag
     5341 gtgcctttca cccaaccaaa ggaatttgag gttgtggggt tggaagccga aaacgtgatt
     5401 attggctacc gtttcagggg tgccaactca cccgataacg atatgctcga ccttatttcg
     5461 gcaatacttt acaacgggcg tgccggatta atcgacctca acatcaacaa gaagcagctg
     5521 acgcttggat gtcaagccta caacagggct atggccgact attcaattct tcaattttca
     5581 gggcgtaaca aaaagggaca gtcgctcgag gaggttaaaa acttactgct ccagcaggtt
     5641 gagctgctta aaaagggcga ttttcccgac tggatgctcg aagctgccgt taacaacgta
     5701 aagctgcgcc aaatgtccca actcgaaagt gcacgcggta gggctagttt gatgtactca
     5761 gcctttttga acaacattga atgggccgat gaggttgcct acatttccag attggaaaaa
     5821 attaccaaag agcaggtggt tcgctttgcc aacgataacc tgggtagcaa ctacgttgtg
     5881 gtttataagc aacagggtaa gcccgaggag gttgccaagg ttgccaaacc gcccataact
     5941 ccaatctaca tcaaccgcga tgccgaatcg gatttcctga aaatgattaa aaactctacc
     6001 gttaaaccaa ttgaaccaag gtttattgac tacgataagg atattaccaa attcaagctg
     6061 aacacgaacg tagaggtgct ttataccaag aatgccgaaa acagcacctt caacctgact
     6121 ttatactacc catttggaag attggctaat cctttgctaa ctcatgcagc cgactacatc
     6181 cagctgcttg gaacttcaaa gcttaccgcc gaacagctaa gccaggagtt ctacaagcta
     6241 gcttgcaact tcaacatttc tgtcagggac gaggagatgc taatttcgtt gacgggcctg
     6301 agcgataatg ccgataaagc ctttgagctg atggaaaacc ttcttgccga ttgccagccc
     6361 aacagcgatg ccctctctat gtacattacc aaccagctaa aatcgagaga cgatgccaag
     6421 aaacggcagc aaagtgtgtt tagcgcattg gttagctatg ctacttatgg agaggaaaac
     6481 ccacaacgct taattctctc aaatgaagag ttgaagggtc ttgctgccca accgctggtt
     6541 gacctaatca gggagtttac ctcatacccg cacatcattc tttactatgg acctcaggat
     6601 gttaagggga ggaaaaaaat gctcaccgaa aagcatattc aacctaaaaa gtacaaatcg
     6661 ttgccggtga acaaaaagtt tgaggagact tcaacaacta ccgaaagggt gctctttgta
     6721 aactacgatg ccaagcaatc gtaccttcaa accattagta agggcgagct ttacagcgac
     6781 aacctttatc ctgtggtaac cctttacaac gaatattttg gtggtggtat gaatgccatt
     6841 gttttccagg aaatgcgtga gaaacgtggc ttggcctaca ctgcacgttc tttgtacagt
     6901 attcccgatg agcccaatag ggtattcatg aacacaagct ttattgcaac gcagaacgac
     6961 aaggttattg atgcttttaa tgctttcaac gagcttttca acaacatgcc tgaagcggaa
     7021 attggtttta acctggcaaa ggaatcgctc atctccaaca tacgttcaca gcgaatcatc
     7081 aggatggcct tactttacaa ctacctagat gcccgtcgaa tgggctggaa tcgcgatatg
     7141 cggcaaaccc tttttgaggt tattccggga atgactctta acgatgtgcg cttattccag
     7201 caaacccatg taaaggataa gcctaaaacc tacgttattc ttggtaagga gtctgacatg
     7261 gatttcaata gactttcgtc gctgtttgga cctgttacca agctctcgct tgaggatatt
     7321 tttggctact agaatgggaa aacaaaaaag ccgattgttc aatcggcttt ttttacacct
     7381 agcctatcat gactgcctaa gcgcagggtt agaacgggtg tataagtgca agttttttag
     7441 aataacttta ctagtacata caaattgcga gtattatgtt gaaaaggagt aaaatgttgt
     7501 tgttactggc gcttgtttat tttaccggca gtggccagga caggatgaca gggcttaact
     7561 ttgccacccg ttcggaggtg attgcgagtc acggta
//
LOCUS       DAOPHX010000117         7452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_12690_length_7452_cov_6.839259, whole genome shotgun sequence.
ACCESSION   DAOPHX010000117 DAOPHX010000000
VERSION     DAOPHX010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7452
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12690_length_7452_cov_6.839259"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PK502_08630"
     CDS             <1..205
                     /locus_tag="PK502_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00920.1"
                     /translation="GSSFQTYSAPSGLVKIVRSIQGAAPLATYYAPSGLLFKRSPGSS
                     FQNYSAPSGLVKIVRSMQGVSIS"
     gene            complement(239..1471)
                     /locus_tag="PK502_08635"
     CDS             complement(239..1471)
                     /locus_tag="PK502_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HPH00921.1"
                     /translation="MKKKLKVILLTVSCMAIALGISASNPQNVKGHPEEYVLLISFDG
                     FRWDYPTMYHTPNLDLIGIEGVHAKSLISCYPSKTFPNHYSLATGLYPDHHGIVNNSF
                     FDKKLGYYSIGNRKSVENGEFYQGEPIWVTAEKQGIRTASYYWVGTEAPIQGIQPTYW
                     KPYNERITFEQRVDTVLHWLSLPEGKRPHLITFYYHEPDLVSHNFGPSSPQTKKVVEQ
                     LDSLLGYFMLKLKLLPIYPKINVIVVSDHGMAEISSDRYINLSEHLKKEWFEYISGGN
                     PVYSLQPKIEHYAQVMDSLKKILHLNVWERGQIPSRYSYGTNPRVNDILIEAEAGWSI
                     GWSSKADDYNGGTHGYDNMLPDMQGIFYAIGPAFKHGYSCASFMNVDVYPLIAHILEL
                     NPAKTDGKLKEVKNMLRQ"
     gene            complement(1701..2108)
                     /locus_tag="PK502_08640"
     CDS             complement(1701..2108)
                     /locus_tag="PK502_08640"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPH00922.1"
                     /translation="MLTQKTRYSMLAMIRLAREYGKGTLLINQIAESERIPKRFLEAI
                     LLELKNNGYLGSKLGKNGGYYLLKKPEEISLLEIIRLFEGSISLLQCTSERFYQPCEH
                     CKDENSCPLRTVFGDIRSYTFNRLDETTLDKLL"
     gene            complement(2111..2800)
                     /locus_tag="PK502_08645"
     CDS             complement(2111..2800)
                     /locus_tag="PK502_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPH00923.1"
                     /translation="MHPNINKNEFKWLRRISMKFINRRDFIRIERSSVSLAFKKGETI
                     LKQGERPSHVVYLERGIVKFSYQNEAGKNLILTIVAAPKILGGANLFYKDNNLFSFVA
                     VDDCDVVLIDAQVLLSVMENNAKFSIMLFQLASGMFKKSILNFISLAYKQKEGRIADI
                     IIYLSAEVYNSRDFTLTLTRRELAEFAGCSTENVIMTLSKWQNENVIAAEGKQLQIID
                     MERLKHISKIA"
     gene            2925..4208
                     /locus_tag="PK502_08650"
     CDS             2925..4208
                     /locus_tag="PK502_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HPH00924.1"
                     /translation="MSRLFKILLPLFFTIVFTPRLYAQFRIDAQLRTRFELRNGYQML
                     AAKGEDPAALVSQRTRIIFSYETDNLKLRITPQDVRLWGGDKNVNSTGITANTELGLF
                     EGFAEVKLGSVGWMSVGRQQLVYDSRHLLGNRNWNQNGISYDALLFKLALSGWNLHVA
                     GSWNTLNETSSNNYYPDSRIKSYNFVWVNHKFPGNFNLSLLHIASGVTETDSTNTLHF
                     RQTTGFYIQYPGEDLKFWANAYYQYGRNKLGKTVSAFLMDADMCYSIGKLTPGIGFGY
                     LSGNSHVGAGQSTDHLFDILYGNRHTFFGFMDYFRSFDKDTRQGGLADYYLYMDFKFS
                     KSVSLCNTAHYFMLAQTNTTTPDDRKLGYENDLILTCKFADWGGFECGYLFMLPAKSL
                     KEIQDVSNSKFSQFLYLQLTITPVIYKQQNESRSL"
     gene            4305..5243
                     /locus_tag="PK502_08655"
     CDS             4305..5243
                     /locus_tag="PK502_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPH00925.1"
                     /translation="MKKSGIIYLLAVSLLLSACASNKQKQRTITISGAFALYPLVVTW
                     AEKYKEENPRVRFNISGGGAGKGMADALAGAVDLGMVSRSIAQEEISRGAWCVGLTID
                     AVVPTISVRNPHLNQILTRGLTREEFSAIFLKESITSWNKLLGIPGNDGITVYCRADA
                     CGAAETWANYLGGKQENIKGVGIFGDPGLAEAVANDPLGIGFNNTIFAFDIKTGKKRS
                     GVEVVPIDVNGNGQLDSTENFYTSLDLILKAISDGSYPSPPARELYFVSRGKPEKKAT
                     INFIRWCLTEGQKYVREAGYVPLNHEKINQYLKLLE"
     gene            5250..6134
                     /gene="pstC"
                     /locus_tag="PK502_08660"
     CDS             5250..6134
                     /gene="pstC"
                     /locus_tag="PK502_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPH00926.1"
                     /translation="MFRRIFFDRTTKTWMITTMVIILLMPVIIGVGLYLKSLPLLEHQ
                     SLRTLLFTSDWQPSQGKFGFWPFVAGSIYVTLIAFVLSAPVCLLAGIYLTQFASSRLV
                     KVMYPVIDILAGLPPVIYGVWGILVVVPFISKFLAPIFGTTSSGYTIMAGGIVLAVMC
                     VPYMLNMITEVFKTVPMGLREASLSLGATFWESVKHVVIRKSYPGIISAFGLGIAKAF
                     GETMAVMMVVGNRVQVHFNPFEAGYPLPALIANNYGEMLSIPLYDSALMFAALLLLVI
                     ILLINLIFRYFIFKTRER"
     gene            6131..6985
                     /gene="pstA"
                     /locus_tag="PK502_08665"
     CDS             6131..6985
                     /gene="pstA"
                     /locus_tag="PK502_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPH00927.1"
                     /translation="MNRWKIIEEKIFIALSAIATYSLIAVLGLIIVIVFVKGFNALSL
                     DMVIKTPKGGYYYGGEGGVLNAIVGSIYIAFGATLIAIVLGMPAALFLNVHLVRHKKT
                     QETIRYLLDVLWGIPSIVYGAFGFTLMLFFGLNASLLAGIITVALLVTPIMVRTFDEV
                     LSTIPRGLHEASLALGSTKAETSYKVMLKQGFSGFITAALLAFGRGIGDAASVLFTAG
                     YTDLIPVNLDEPAATLPLAIFFQLGSPIPEVRERAYAASTILTIIILIISLSARFLSN
                     RYSKTNIK"
     gene            7010..>7452
                     /locus_tag="PK502_08670"
     CDS             7010..>7452
                     /locus_tag="PK502_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPH00928.1"
                     /translation="MTNNPTDTQVIQDCTILSIRNLNVHAGNHHILKDINLEIPKNRI
                     TVLLGPSGCGKTTLLKCMNKLTDLYRELKVTGNIFIEDDDILNTSKNIPSIRQKMGLL
                     SQRPYPLPVSIYKNVAYGIKLKGIRDRKLIDYNVEKHLKEVGLWN"
BASE COUNT         1897 a         1656 c         1707 g         2192 t
ORIGIN      
        1 aggttcttcg tttcaaacct attccgcccc ttcagggctt gtaaaaatcg ttcgctccat
       61 acaaggggct gcgccccttg ctacttatta cgccccttcg gggctcttgt ttaaacgctc
      121 tccaggctct tcgtttcaaa actattccgc cccttcaggg cttgtaaaaa tcgttcgctc
      181 catgcaaggg gtatctattt cttaatcctc aaatcttaat ttaacttttt tgatttccct
      241 attgtctcaa catatttttt acttctttaa gtttaccgtc ggttttcgcc gggttaagct
      301 ctaatatatg ggcaatcaac gggtaaacat caacattcat gaacgaagca cagctgtatc
      361 catgcttaaa tgccggtcca atggcgtaga atattccctg catgtcgggg agcatgttgt
      421 catacccgtg tgtacccccg ttgtaatcat cggcttttga cgaccatcct atgctccatc
      481 ctgcctcggc ttcgatgagg atatcgttca ctcttgggtt ggtaccgtag gaataccttg
      541 agggtatttg gcctcgctcc caaacattta ggtgcaaaat cttttttagg ctatccatga
      601 cttgggcgta atgctcaatt ttgggttgaa ggctgtaaac cggattacct cctgatatat
      661 actcaaacca ctcttttttt aaatgttccg aaaggttaat gtacctatcg cttgaaattt
      721 ctgccattcc gtgatccgaa acaacaatca cgtttatttt agggtaaatg ggaagcaatt
      781 taagtttaag cataaagtag cccagcaggc tgtcgagctg ttcaactacc tttttggttt
      841 ggggcgatga cgggccaaaa ttatggctca ccaggtcggg ctcatggtag tagaaggtga
      901 tgagatgagg tcgttttcct tcgggaagcg atagccagtg aagaacggtg tcaacacgct
      961 gttcgaaggt aatcctttcg ttgtatggct tccagtatgt gggttgaata ccctgaatag
     1021 gtgcttcggt tcctacccag tagtaagagg ctgtcctaat gccttgtttc tcagcggtta
     1081 cccagatggg ctcaccctga tagaactccc cattttccac actcttcctg ttaccaatgg
     1141 agtagtaacc aagctttttg tcaaaaaatg agttattcac aattccatgg tggtcggggt
     1201 acaggccggt cgccaggctg tagtggttgg ggaaagtttt ggaggggtag cagctaatta
     1261 acgatttggc gtgaacaccc tcaattccga tcaggtcgag attgggagta tggtacatgg
     1321 tggggtagtc ccaccgaaaa ccgtcaaatg agataagcag aacatactcc tcaggatgac
     1381 ccttcacgtt ctgcggattt gatgcgctaa tgcctaaagc aatagccata cagcttactg
     1441 tcagcaaaat aacttttaat tttttcttca tttctttaca accttataag gtaatggatg
     1501 gctttgcctg aaattgaaac tcgtagtaca aacacataaa tcataatttg ttcaacagta
     1561 ttagtattgt ttccctgaaa gtttccctct tgccgtaaag ttaacctgta tgatttctaa
     1621 cgtgtaatac ggtgggttaa ctccgatata gttcgacacg gtctaagagt tttggtttta
     1681 tgcacaggag aaaaagttct tcataaaagt ttatcgaggg tagtttcgtc caacctgtta
     1741 aatgtgtagc ttcttatgtc gccaaaaact gtgcgcagcg ggcaggagtt ttcgtccttg
     1801 caatgctcgc atggctggta aaacctttcg gaagtgcact gaaggagcga gatgcttccc
     1861 tcaaaaagcc ttatgatttc cagcaggctt atttcttctg gtttcttaag caggtagtac
     1921 ccgccgtttt tacccaactt gcttccaagg tagccattat ttttaagctc cagcagaata
     1981 gcttcaagaa aacgtttggg tattctctcg ctttcggcaa tttgatttat caacagggtt
     2041 cctttgccgt attccctggc cagccttatc atggccagca tcgagtatct tgttttttgg
     2101 gtcaacatgg ctaggcaatt ttgctgatgt gctttagtcg ttccatatca attatctgta
     2161 attgcttacc ctcagctgct atcacatttt cgttttgcca tttcgagagg gtcataatga
     2221 cattttccgt tgagcatccg gcaaattctg ccagctccct ccgggtcagg gtgagggtaa
     2281 aatctctcga gttgtaaacc tcggctgaga ggtatatgat gatatcggct attcgcccct
     2341 ccttttgctt gtacgccagg ctaatgaagt tcagtatgga ctttttaaac atgcctgagg
     2401 ccagctgaaa cagcataatg gagaacttag cgttgttttc cattaccgac aggagaacct
     2461 gagcgtctat gagtaccaca tcgcaatcat caacggcaac aaatgaaaag aggttattgt
     2521 ccttgtagaa taggttagcg ccaccaagta tttttggtgc tgcaacaatg gtaagtatca
     2581 ggtttttgcc tgcctcattc tggtagctaa acttcactat gcccctttcg aggtaaacta
     2641 cgtgggatgg ccgttccccc tgtttcagga tggtttctcc ctttttgaag gctaagctaa
     2701 ccgacgacct ttcaattctg atgaaatccc tgcggttgat aaacttcatc gaaatccttc
     2761 gcaaccattt gaactcattt ttgttgatat tagggtgcat tagttatagg ctgtttggaa
     2821 aaagtattaa atatttaatg ctgttatgca taaatatgga gaacaaatat acactaaacc
     2881 tattgattta atgtttaagt ttgcctggct aaatttacaa accgatgagt aggctgttta
     2941 aaattttact acctcttttc ttcaccatag tctttacacc ccgcctatac gcccagttta
     3001 ggatagatgc ccagcttcgg actcggtttg aacttcgcaa cggctaccag atgttggctg
     3061 ccaagggcga agaccctgcg gctctggttt cgcagcgcac ccgtatcatt tttagctacg
     3121 aaacggataa tctgaaatta aggattaccc cgcaggatgt gaggctatgg ggaggcgaca
     3181 aaaacgtaaa ttcaaccgga ataaccgcaa acaccgagtt gggcttgttt gaaggatttg
     3241 ctgaggttaa gctgggaagt gttgggtgga tgtcggtggg taggcagcag ctggtttacg
     3301 atagccgcca tttattggga aataggaatt ggaatcaaaa tggtatcagc tacgatgccc
     3361 tccttttcaa gctggctcta tccggctgga atttgcatgt tgccggctcg tggaacacgc
     3421 ttaacgagac atcatccaac aactactacc ccgactcacg catcaaatcc tacaatttcg
     3481 tttgggttaa ccataaattc cctggcaact ttaacctatc cctgctccac attgcttctg
     3541 gggtaaccga aacagattca accaataccc ttcatttcag gcaaaccacc ggattctaca
     3601 tccagtatcc cggagaggat ttgaagtttt gggcaaatgc atactaccag tatggtagaa
     3661 ataaactggg aaaaacggta agcgcttttt tgatggatgc cgatatgtgc tactcgattg
     3721 gaaagctgac gcccgggata gggtttggct acctatcggg taacagccat gttggtgccg
     3781 gacaatcaac cgaccacctt ttcgatattc tctacggtaa ccgccacaca ttttttgggt
     3841 tcatggacta cttcagaagt tttgacaagg ataccaggca aggcggattg gccgactact
     3901 acctctacat ggatttcaag ttctctaaat cggttagcct ttgcaacact gcccattact
     3961 tcatgctggc ccaaaccaac acaactaccc ctgatgaccg aaagttgggg tatgagaatg
     4021 atttgatatt aacctgcaag tttgctgatt ggggtgggtt tgagtgcggt taccttttta
     4081 tgctgcccgc caaatcgttg aaggagattc aggatgtttc caacagcaaa ttctcccagt
     4141 tcctgtactt gcagctcact attactccgg tgatttacaa acaacaaaac gaatctaggt
     4201 ctttatagca aataggaatg tggaatttat atattgatat tcaacttaat gtggctaaat
     4261 tgtaaagcgt tacatttata ttgcaaaaga tacactctac tgacatgaaa aaatcaggaa
     4321 ttatttacct cctagctgtc tcgttgctcc tatcggcatg tgcctcgaac aagcagaagc
     4381 agcggactat aaccatttct ggagcatttg cactctatcc gctggtggta acctgggccg
     4441 aaaagtacaa ggaggaaaac cccagggtga gatttaatat ttccggaggt ggcgcaggta
     4501 agggaatggc agatgccctt gccggtgctg ttgatcttgg tatggtatcc cgatcaattg
     4561 ctcaggagga aattagcagg ggtgcatggt gcgttggatt gaccattgat gccgttgtgc
     4621 ccaccattag cgttagaaat ccccatctga atcagatttt gacgcgaggg ctaacgcgtg
     4681 aggagttttc cgcaatattt cttaaagaat ccataacgag ttggaacaag ctgcttggca
     4741 tccccggtaa cgatggcatt actgtgtact gccgtgccga tgcctgtggt gctgccgaaa
     4801 cctgggcaaa ctacctgggt gggaagcagg aaaatattaa gggagttgga atttttggtg
     4861 acccaggact tgcagaagct gtggccaacg atcctcttgg tattggcttt aacaacacaa
     4921 tttttgcctt cgatataaaa accggaaaaa agagaagcgg ggttgaggtt gttccaatcg
     4981 acgtcaatgg gaacggtcag cttgattcta cagaaaattt ttacacttcc cttgacctga
     5041 ttctaaaagc cattagcgat gggagttatc cttcgccacc cgctcgcgag ctttactttg
     5101 tttccagggg taaacctgag aaaaaagcta caattaattt tataaggtgg tgccttaccg
     5161 aaggtcagaa atatgttagg gaggcggggt atgtacccct taaccatgaa aagataaacc
     5221 agtatttgaa gctgctggag taaaaagcca tgtttcgaag aatatttttt gaccgtacca
     5281 ctaaaacatg gatgattacc acgatggtaa tcatcctgct tatgccggta attattgggg
     5341 ttgggcttta cctgaaatca ctgccgttgt tagagcacca atccctccga accctgcttt
     5401 tcacctccga ctggcagcca tctcagggta agtttggctt ctggccattt gtggcgggtt
     5461 ctatctacgt aaccctgatt gcatttgtac tatcagctcc agtttgcctg ctggccggca
     5521 tttaccttac tcaatttgcc tcatcaagac tggtcaaggt gatgtacccg gtaattgata
     5581 tattggctgg gctaccacct gtcatttacg gtgtttgggg tattctggtg gtggtgccct
     5641 tcatttcaaa atttcttgct cctattttcg gaaccacctc ctcgggctac accattatgg
     5701 cgggaggtat tgtgctggca gttatgtgtg tcccctacat gctgaacatg ataaccgaag
     5761 tttttaaaac ggtgccaatg ggattgagag aggcttccct ttcactgggt gccacctttt
     5821 gggagtcagt aaagcatgtg gttatccgca agagctaccc ggggataatt tcagcttttg
     5881 ggctgggcat agccaaggca tttggcgaaa caatggctgt gatgatggtg gtgggtaacc
     5941 gggtgcaggt gcacttcaac ccctttgaag ccggatatcc tttaccggca ctcattgcca
     6001 ataactatgg cgaaatgctt tccattcccc tgtatgattc agcgctgatg tttgccgcct
     6061 tgctgctgct ggttatcatt cttttgataa acctgatatt tcggtacttc atctttaaaa
     6121 cccgcgagcg atgaacaggt ggaagattat agaggagaaa atatttatag ctctttcggc
     6181 gattgccacc tactccctta ttgctgtttt gggcctaatc atcgtcattg tgtttgtcaa
     6241 ggggtttaac gccctgtcgc tcgatatggt gattaaaaca cctaagggag gatactacta
     6301 cgggggagag ggtggagtcc tgaatgccat tgtaggttca atttacattg cttttggcgc
     6361 aacgcttatt gccattgtac tgggtatgcc ggctgccctt ttcctgaacg ttcacctggt
     6421 aaggcacaaa aaaacgcagg aaaccattcg ctacctgctc gatgtgctat ggggcattcc
     6481 atccattgtt tacggggcat ttggttttac gctgatgctt ttctttggtt taaatgcatc
     6541 gttgctggca ggaattatta cagttgccct tctggtaacc ccaattatgg tgcgcacctt
     6601 cgatgaggtt ctttcaacca taccaagggg gttgcatgag gcaagcttgg ccttgggctc
     6661 aaccaaggcc gaaacctctt acaaggtgat gctgaagcag ggtttctctg gattcatcac
     6721 tgctgcattg ctggcgtttg gcaggggcat tggcgatgcc gcttcggtgc tttttactgc
     6781 aggttacacc gatttaatac ctgttaacct tgatgagcct gccgccacgc tgcctttggc
     6841 tatattcttt cagctgggct ctccaatccc tgaggtaagg gagcgtgcct atgctgcaag
     6901 cactattctc accatcatca tcctgattat aagccttagc gcaaggttcc tttcgaaccg
     6961 atattcaaaa actaacatta agtagttcat cttagaaatt gatattaaaa tgaccaataa
     7021 ccctactgat actcaagtta ttcaggactg tacaatttta agcatcagaa acctaaacgt
     7081 acatgcgggt aaccatcata ttctgaagga tataaacctg gaaattccta aaaataggat
     7141 aacagttctt ctaggtcctt cgggttgtgg caaaaccacc ctgctaaagt gcatgaataa
     7201 gctaaccgac ctgtacaggg agcttaaggt cacgggcaac atttttatcg aggacgatga
     7261 catcctgaat acaagtaaaa atataccttc aattaggcaa aaaatgggtt tgctctcgca
     7321 acggccctac ccgttacccg tttcaattta taagaatgta gcctacggga taaagcttaa
     7381 ggggatacgc gaccgaaagc tgatagacta caatgtggaa aagcacctta aggaggtggg
     7441 cttgtggaac ga
//
LOCUS       DAOPHX010000118         7388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_12823_length_7388_cov_6.610528, whole genome shotgun sequence.
ACCESSION   DAOPHX010000118 DAOPHX010000000
VERSION     DAOPHX010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12823_length_7388_cov_6.610528"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PK502_08675"
     CDS             <1..774
                     /locus_tag="PK502_08675"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPH00929.1"
                     /translation="RMREFEQMEMQFFVKPGEEMKWFEYWLDKRMRWHKAMGFGDGKY
                     RFHKHEKLAHYANAASDIEFEFPFGFKEVEGIHSRTDFDLSNHQKHSGKKLQYFDPET
                     NESYIPYVIETSIGLDRTFLLVMSAAYTEEKLTKDDGTADERVVLRIPPALAPVNLAI
                     FPLVKKDGLPEMARKIMDDLKYDFTCQYEEKDTIGKRYRRHDAIGTPFCITVDHQTLD
                     DETVTIRYRDSMEQDRVPASKLREIMGEKVSLTNLLKQV"
     gene            complement(833..1447)
                     /locus_tag="PK502_08680"
     CDS             complement(833..1447)
                     /locus_tag="PK502_08680"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPH00930.1"
                     /translation="MHIAVAGNIGSGKTTLTSLLAKYYKWKPQYEDVDDNPYLNDFYE
                     DMERWSFNLQIYFLNSRFSQIVSIHRSGEDVIQDRTIYEDAHIFAPNLHQMGLMSSRD
                     FNNYLNLFNLMTSLVKPPDLLIYLRASVPTLVRQIQKRGREYEKNIRLDYLQRLNERY
                     EAWIEGYNLGKLLIIDVDHLDFSEKPEDLRTVINRIDAQIHGLF"
     gene            complement(1595..3124)
                     /locus_tag="PK502_08685"
     CDS             complement(1595..3124)
                     /locus_tag="PK502_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HPH00931.1"
                     /translation="MPLVNSLLNRINSSRLRSIDYFRRHPQEVQEQTLFNLLSRAKNT
                     EYGQLNGFSRFKSIADFQQGLPVMEYEDFKPYIDRMRSGEENLLWPGKISWFAKSSGT
                     TQNKSKFIPVSREGLNGCHFRGSRDVIAIYFDLYPKNGLFRGKGLTLGGSHQVDNLSG
                     SSRYGDLSAILLQNQPWWTDFIRTPSQRVALIPDWEQKLEKITGETLAQNVTSITGVP
                     SWNLVMIKHILNHTGKSNLLEVWPNLELFIHGGVSFTPYRDQYFKLIPSPQMHYMEAY
                     NASEGFFAIQDNPETDDMLLMLDYGVFYEFIPAEQLGQPNPPAYPIGEVEVGKNYAML
                     ITTSSGLWRYLIGDTVTFTSKSPYKIKITGRTRHFINAFGEEIIIDNAEKAIKKACDA
                     TGAQVAEYTAAPVYMETDKKGSHEWLIEFNVDPASLDEFSRLLDAALCEVNSDYEAKR
                     YKNITLDFPLVRSLAKGTFFEWMKRNNKLGGQNKVPRLSNNRDYANQLLAIHRELSSA
                     S"
     gene            complement(3366..3992)
                     /locus_tag="PK502_08690"
     CDS             complement(3366..3992)
                     /locus_tag="PK502_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HPH00932.1"
                     /translation="MQVEFFHDVLCAWCFALSPRMRRLVAEFPEIEVIHRSFALASDA
                     DRLVEMFGSKEAAKREILNHWRNANENDDEHRIHTEDMALRTFDYPYSMPGLLACKSA
                     ELQGGQLAHWNMFDRIQKAHLVETQNIADFNVLLQCADEVGLDTERFKNDFESDATLD
                     AIYQDSYRAKELGVNAVPSLLINGRKLLSGAHKYETLKELTQRELIGK"
     gene            complement(4014..4862)
                     /locus_tag="PK502_08695"
     CDS             complement(4014..4862)
                     /locus_tag="PK502_08695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPH00933.1"
                     /translation="MKRAITLSIFAIAIAALISCTGNKSNKAGETSTPPTMQPVVKGT
                     EITYYDGSTPLKGYIAYDESLTGKRPGILVVHEWWGNNDYSRMRANMLAELGYVAMAV
                     DMYGNGLILDNPKDAQNTANQFYTNPALMKERMLVAYNTLIADSLVNAERIAAIGYCF
                     GGAVVLNSASMGVPLDAVVSIHGGLTGFKATPEMKNVNVLICHGGADPFVPQADIDNF
                     KSEMQNMGVNYEFKVYEGATHAFSNKASTEAGKKFNMPISYNAAADSASWSDMVTFFN
                     KYFPAK"
     gene            complement(5249..6205)
                     /locus_tag="PK502_08700"
     CDS             complement(5249..6205)
                     /locus_tag="PK502_08700"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HPH00934.1"
                     /translation="MSTKKDFLLVIPFLMISNLITAQISETVVKELKTQSEDSILGWK
                     KGFVTGINFSQTSLTNWAAGGQNSLSLATMLSGFVSFKNEKVAWDNTLDIGYGFLNQN
                     GVKYNKKTDDRVDLSTKYGYRASKNIYYALLLNFKTQMAKGYDYPNDSTRNMISNFFA
                     PAYLVGALGMDIKPSSYLSLFVAPFTGKFTFVTDSILSSTGAFGVKPGKEYRSEFGGY
                     VRISFSKADFKPEVLKNLSFTTKVDFFSNYADKPQNIVVNWEALVAIKVNKYLNMNLS
                     TMLIYDDKIKINIDKNGDGTFDSKSPRVQFKEILGVGLSVKL"
     gene            complement(6242..>7388)
                     /locus_tag="PK502_08705"
     CDS             complement(6242..>7388)
                     /locus_tag="PK502_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPH00935.1"
                     /translation="GYCPGIENYSRYFDGRQAGMRPFCLLDYFPNDFLTIIDESHVTL
                     SQVRAMYGGDNSRKQTLVEYGFRLPAAIDNRPLRFDEFEGLIGQTIFVSATPSDYELR
                     LCQGVVVDQVVRPTGLLDPPIIVRPSLNQIDDLIGEINASAKRDERVLVTTLTKRMAE
                     ELVKYLVKFDIRCRYIHSDVDTLERVQIMEDLRKGLFDVLVGVNLLREGLDLPEVSLV
                     AILDADKEGFLRSARSLTQTAGRAARNLNGRVIMYADKITGSMQLTMDETNRRREKQL
                     KYNELHGITPTQIVKPKKSIMAGKLSTGEVVKPYVEPDTVDFAADPVVQYMTKPELAE
                     AIERARKEMEREAKDLNFFEAARYRDEMFALQRLFETKFGAKKSKST"
BASE COUNT         1814 a         1744 c         1795 g         2035 t
ORIGIN      
        1 cgtatgcgtg aatttgaaca gatggaaatg cagttttttg ttaagcccgg tgaggagatg
       61 aaatggttcg agtattggct cgataagcgg atgcgctggc acaaggccat gggtttcggc
      121 gatggaaagt accggttcca taagcatgaa aagcttgcgc attacgccaa tgcagcctcc
      181 gatatcgagt tcgagttccc atttggcttc aaggaagttg agggtattca ctcccgcacc
      241 gattttgacc tgagcaacca ccagaagcat tccgggaaga agctgcagta cttcgacccg
      301 gaaaccaacg agagctacat tccctacgtg atagaaacct ccatagggct cgaccggacc
      361 ttcttgctgg taatgtcggc tgcctacacc gaggagaagc ttaccaagga tgacggcact
      421 gctgacgaga gagttgttct ccgtattccg cctgcccttg cccctgttaa cctcgcaatt
      481 ttcccgctgg taaaaaagga tggactacca gaaatggctc gtaaaattat ggatgacctg
      541 aagtacgact ttacctgcca gtatgaggag aaggatacca ttggcaagcg gtaccgccgt
      601 cacgacgcca ttggaactcc attctgcatc acggttgacc accaaacgct tgacgacgaa
      661 acggttacca ttcgctatcg cgacagcatg gagcaggacc gtgtgcctgc gtcaaagctc
      721 agggaaataa tgggcgagaa ggtttcgctt accaacctgt tgaagcaagt ttaaccctat
      781 catccggagc gagaggctcc gatacgttac tttttggaag acttgctact tttcagaaaa
      841 gtccgtggat ttgcgcatca atcctgttga ttaccgtgcg caggtcttcg ggtttctccg
      901 aaaagtcgag gtggtcaaca tcaataatca ggagttttcc gaggttgtag ccttcaatcc
      961 atgcctcata ccgctcgtta agccgctgca ggtagtcgag ccggatattc ttctcgtact
     1021 cccttcctct tttctggatt tgcctgacca gcgtgggaac agatgctcta aggtagatga
     1081 gcaggtcggg aggcttcacc agcgaggtca tcaggttgaa caggttgagg tagttgttga
     1141 aatcgcgact cgacatgagg cccatctggt ggaggttggg ggcaaagata tgggcatctt
     1201 cgtaaattgt cctatcctga attacatcct cgccgctccg gtgaatgctc acaatctggg
     1261 agaaacggct gttgaggaag taaatttgca ggttgaagct ccaccgctcc atatcctcgt
     1321 agaaatcgtt caggtagggg ttatcatcca catcctcata ctggggcttc cacttgtagt
     1381 attttgcaag caagctagtt agcgtggttt tccccgaacc aatgttaccg gcaactgcaa
     1441 tatgcataat cttttgtggc ttttagctgg ttgtttgatt ttttaaaggt agcaatttac
     1501 attaaatgaa tggcatgttg tgactggttg tagctgtaac tttgacagaa gtggcataag
     1561 gtagcattaa cagcaaggag gagatggcaa atggctagct ggccgatgac agctccctgt
     1621 gaatagccag cagctgattg gcatagtcac ggttgttgga tagccgtggc accttgttct
     1681 gcccgcccag cttgttgttt cgtttcatcc attcaaaaaa ggttcctttg gccagcgacc
     1741 gaaccaaggg gaagtcgagg gtgatattct tgtagcgctt ggcttcgtag tccgagttaa
     1801 cctcgcaaag agcagcatcg agaagcctcg aaaattcatc caggctggca gggtcaacgt
     1861 taaactcgat aagccattcg tggctaccct ttttatcggt ttccatgtaa accggggctg
     1921 cggtgtactc ggcaacctgt gccccagttg catcgcaggc tttcttaatt gctttttcgg
     1981 cgttatcaat aatgatctcc tcgccaaaag cgttgatgaa atgtcgggtt cgcccggtta
     2041 tctttatttt atagggtgat tttgaggtga aggttaccgt atcgccaatc aggtatcgcc
     2101 acagccctga actggtggta atcagcatgg cgtaattttt acccacctca acttcaccaa
     2161 tggggtaggc cgggggattg ggctgcccca gctgttcggc aggaataaac tcgtagaaaa
     2221 cgccatagtc gagcatcagc agcatatcat cggtttccgg gttgtcctga atggcaaaaa
     2281 aaccctcact ggcgttgtag gcctccatgt agtgcatctg gggtgaggga attagcttaa
     2341 agtactggtc gcggtaaggc gtaaagctaa cgccaccgtg aatgaatagc tccaggtttg
     2401 gccaaacctc cagcaggttc gactttccgg tgtggttcag aatatgctta atcatcacca
     2461 ggttccatga tggcacaccg gtaatgctgg ttacgttctg ggccaaggtt tcgccggtaa
     2521 tcttttccaa cttctgctcc cagtcgggga taagggcaac ccgttggctg ggagtgcgga
     2581 taaaatcggt ccaccagggt tggttttgca ggagtatagc cgataaatcg ccgtagcggg
     2641 agctgcccga aaggttatcc acctgatggc ttccgcccag ggtaagtccc ttgcctctaa
     2701 aaaggccatt tttagggtac aggtcaaagt aaatagctat cacatcgcgg cttcccctga
     2761 agtggcatcc gttcaacccc tcccggctta ccggtataaa tttactcttg ttctgtgttg
     2821 ttcccgaaga tttggcaaac cagctaattt taccgggcca aagcaggttc tcctcaccgg
     2881 atctcatccg gtctatgtag ggtttgaagt cttcatactc cattacaggt agtccctgtt
     2941 gaaaatcggc tatggatttg aacctgctaa atccgttcag ctgtccatac tccgtatttt
     3001 ttgccctgga aagcaggttg aaaagggttt gctcctgcac ctcctgcggg tgccttcgga
     3061 agtagtcaat gctgcgtaaa cggctggagt taatccggtt gaggagtgaa ttcaccaaag
     3121 gcataccctt aaagttgttc gatttcctag caaatttaaa ctaaatctac aaatttccat
     3181 ccctttgcat aggagaagta aacaaatgcc ttcaacaacc ggtatggtac ggttgtctcc
     3241 ccggaccggc gttcttctca attaatatgg gtttataccg tatagaagat gcaaaaaaaa
     3301 agtctgacca gggtgttgaa caagttctag ttcttcaacc cggtcagacc agagcacaaa
     3361 ccaagctatt ttcctataag ttctctctga gtcagctcct ttagtgtttc gtacttatgt
     3421 gctccgctta gcagcttcct gccgttaatc agcagggaag gaacagcatt aacacccagc
     3481 tccttggccc tgtatgaatc ctggtaaatg gcatcaaggg tagcatccga ctcaaagtcg
     3541 ttcttaaaac gttccgtatc aagccccact tcgtcagcac attgaagcag cacattaaaa
     3601 tcggctatgt tttgtgtttc aaccaggtga gccttttgta tcctgtcgaa catattccaa
     3661 tgcgccaatt gcccaccctg cagctcggct gacttgcagg cgagcaggcc tggcatggag
     3721 taggggtagt caaatgttct tagcgccatg tcctcggtgt gtattctatg ctcatcatca
     3781 ttctcgttgg catttcgcca gtggttgaga atttccctct tggcagcttc cttactgcca
     3841 aacatttcta ccagcctatc agcatctgat gcaagggcaa aagagcgatg tataacctcg
     3901 atttcgggga attctgccac cagcctccgc atacgtgggg aaagtgcaaa gcaccaggca
     3961 caaagaacat catgaaaaaa ttcaacctgc atagtggcaa cttttcatgt aagttatttt
     4021 gcggggaagt acttattgaa gaatgttacc atatcgctcc acgaagccga atcggcggct
     4081 gcattgtagg aaatgggcat gttgaacttt ttaccggctt cggttgaggc tttattggag
     4141 aaggcatggg tggcaccctc gtaaacctta aactcgtagt taacccccat attctgcatt
     4201 tcactcttaa agttgtcaat atcagcctgt ggaacaaagg gatcggcgcc gccatggcaa
     4261 atcagcacgt tgacgttctt catctcgggg gtagctttaa atccagttaa tccaccatgt
     4321 atgcttacca ctgcatccag gggaacaccc atgctagctg agttaagcac aacagcacca
     4381 ccaaaacagt agcctattgc agcaatacgc tcggcattaa ccaggctgtc ggcaattagt
     4441 gtgttgtagg ccaccaacat gcgctccttc atcaaggctg gatttgtgta aaactgattg
     4501 gcagtgtttt gggcatcctt ggggttgtcg agtatcaacc cgttgccgta catgtcaact
     4561 gccatggcta cataaccaag ctcggccagc atgttggcgc gcattctgga gtaatcgttg
     4621 ttgccccacc actcgtggac caccagtatg cctggacgct ttccggttaa actttcgtcg
     4681 taggcaatgt agcccttaag tggggttgag ccgtcgtagt aggtaatctc tgttcccttt
     4741 actacaggtt gcatggttgg aggagtagaa gtttcacctg ctttgttact tttattcccg
     4801 gtgcaggaaa tcagcgctgc aattgctatt gcaaaaattg ataaggtaat tgctctcttc
     4861 ataatataat ttttttttca aacttataac aacgggtaca gtcaaaaagt tcaactatgc
     4921 ttatggcagg gagttacaat agctggaatt aggtggtatt ttttagtcat caggcgaatt
     4981 tatgcaagcg cagctcttgc agattataga tattcaggat aatagcaggc caagcatgca
     5041 cgaacggccg cctcaccaaa agttatggaa ggctaacgga caaaaatgtg atttagagaa
     5101 gttttagcca gggttagatt ttttcgtact tctcagggaa tatgcaccaa tgatgaggat
     5161 ctgcaaagca ccttgcacgt cgggacgaac cacctgcaac cctgctaaaa ttatgactac
     5221 aggaaaatgt ttgtattagc ccgaaaacct atagcttaac agatagacca actcctaaaa
     5281 tttctttgaa ttgaactcgt ggggactttg agtcgaaggt accatctccg tttttatcaa
     5341 tgttaatttt aatcttgtcg tcgtagatga gcatggtgct caggttcatg ttgaggtact
     5401 tgttcacctt aattgcaacc agcgcttccc agtttaccac tatgttttgg ggtttgtcgg
     5461 catagtttga gaaaaaatca accttggtgg tgaacgatag attttttaaa acctcaggct
     5521 taaagtcagc ctttgagaag cttatccgca cgtagccacc aaattccgat ctatactcct
     5581 tgcccggttt taccccaaag gcaccagtgc tcgacaggat ggaatcggta acaaatgtaa
     5641 attttcctgt aaacggagca acgaaaaggc ttaggtagct gctgggtttt atatccatgc
     5701 cgagcgcacc caccaggtat gcgggggcaa aaaagttcga aatcatgttt cgggtgctat
     5761 cgttggggta gtcataccct ttcgccattt gggttttaaa gttcagcagc agggcgtagt
     5821 aaatattttt tgaggctcta tacccgtact tggtggaaag gtcaactcta tcgtcggttt
     5881 ttttattgta ctttacccca ttctggttga ggaacccata gcctatatcg agggtgttat
     5941 cccatgccac cttttcattc ttaaagctga cgaacccgct aagcatggtg gcaagcgata
     6001 gggaattttg tcctcctgcc gcccagtttg tcagcgaggt ttgtgagaag ttgattccgg
     6061 ttacaaatcc cttcttccag ccaaggattg aatcctcact ttgagttttc aactccttta
     6121 ctacggtttc ggaaatttgt gctgtaatta ggttgctaat cattagaaac gggatgacaa
     6181 gcagaaaatc ttttttagtg ctcatggttt cggttattac aatgacattg tgggtgattt
     6241 tttaggttga tttggatttt tttgctccaa attttgtttc gaatagcctt tgcagggcaa
     6301 acatttcatc gcggtatcgg gcggcttcaa aaaagttgag gtctttggct tccctttcca
     6361 tttcctttct tgcgcgttca atggcttcag ccaattcggg ttttgtcatg tactgaacaa
     6421 cgggatcggc ggcaaagtcc accgtgtcgg gctcaacgta gggcttaacc acctcgccgg
     6481 ttgaaagttt tccggccatg attgattttt tgggcttgac aatttgagtc ggggtaattc
     6541 catgcagctc gttgtatttc agctgctttt cgcgacgcct gttggtctca tccatggtaa
     6601 gctgcatgga gcctgtgatt ttatcggcat acatgattac ccgtccgtta aggttacggg
     6661 cagcccgacc tgcggtttga gtaagcgagc gtgctgagcg caggaaaccc tccttatcgg
     6721 catccagtat ggctaccagc gaaacctcgg gcaggtcgag tccctcccga agtaggttta
     6781 cgcctaccag cacgtcaaat agcccttttc gcaaatcctc cattatttga acccgttcca
     6841 gggtatccac atcggaatga atataccggc aacgaatgtc gaacttcaca aggtacttta
     6901 ccagctcttc agccatacgc ttggtgaggg tggttaccaa aaccctttcg tcgcgttttg
     6961 cactggcatt tatttcacca atcaagtcgt caatttggtt aaggctaggc ctgacaataa
     7021 taggtggatc cagcaggccg gttggcctga ccacctggtc aaccaccacc ccctggcaca
     7081 gccgaagctc atagtctgat ggtgttgcgc ttacaaaaat ggtttgcccg attaagccct
     7141 cgaattcgtc gaatcgaagt ggacggttgt caatagcggc aggtagccta aagccatact
     7201 ccacgagcgt ttgcttgcgc gagttatcac ctccatacat cgccctgacc tgcgaaaggg
     7261 ttacgtggct ttcgtcaatt attgtcagga aatcatttgg aaagtagtcg agaagacaga
     7321 aaggcctcat cccggcctgc cggccatcga agtagcggga gtagttttct ataccagggc
     7381 agtagccc
//
LOCUS       DAOPHX010000119         7354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_12913_length_7354_cov_5.232361, whole genome shotgun sequence.
ACCESSION   DAOPHX010000119 DAOPHX010000000
VERSION     DAOPHX010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7354
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12913_length_7354_cov_5.232361"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..834)
                     /locus_tag="PK502_08710"
     CDS             complement(19..834)
                     /locus_tag="PK502_08710"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPH00936.1"
                     /translation="MKSKLILALMAVGCTLNAAAQEFDSVIIKQHVYTLASDSLMGRG
                     FGTTGGKMAADYITAQLEAAGVMPWQGSYLHPFISSGIMVKTEGANIIGWVEGTDPLL
                     KDEYIVIGAHYDHLAYKITDDGKRIVFNGADDNASGVAAIIEIGRWLAGSRNQLKRSV
                     ILAAFDGEEAGLVGSSFMVQHNQVPPKKVKVMFSLDMVGMLSRYGGIDLNGDKTLENG
                     DVMLHNLASKHGLTVKKDGKNVVPNTDTAPFGNQGIPAIHIFIILGFMVGRYG"
     gene            complement(982..2574)
                     /locus_tag="PK502_08715"
     CDS             complement(982..2574)
                     /locus_tag="PK502_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPH00937.1"
                     /translation="MKRIITLLFVLAGLMAHSHAQSDSVIQKIIQIGTAENQTMKHLD
                     VLCNQIGGRPVGSSAYEDAVVWAARQFQAWGMDVFLDEVDELPVGFNRGPWFGRLLGD
                     HSMTLHFATPSYTAGTKGVQRGHVVAEPKTQREFERMKGKLKGAWVLISGESNGWPID
                     YSVRANHRRDSIIASNNEIEKKNDEIQRENWMNRSANQPPKELIPLTEEPGLFYRQMV
                     EAGILGIIQSAPNPIRVLYDRKNINSMVWDSLPQVPDIKLDENQYKIIERMARERQYF
                     QLEFDIRNHFRPGPIPYHTVVGVIKGTEFPNEYVIMGGHLDAFDVATGAADNASGVAP
                     AMEAARLIMKAGGKPKRTILVCLWAGEEFGLLGSTSWVIKNSDKLQSISAMFNRDGGP
                     TVASNLSVPKAMLNDFSGICSPLNSINPSFPFTLKEATPRPKPKNPGGTDTAPFLMAG
                     VPAFQFGTEDVDGLDFSYQEIWHTERDTYEKCPADYMNHTSIVTAIVVYGVANLDHLL
                     SRENLFAPDDKPKVNGKAKGKK"
     gene            complement(2770..4266)
                     /locus_tag="PK502_08720"
     CDS             complement(2770..4266)
                     /locus_tag="PK502_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HPH00938.1"
                     /translation="MGSIQLELALLILSTLFFLSILAGKVSSKYGVPALLLFLIVGML
                     SGSDGLGIQFENIHISQAIGTIALCVILFSGGLDTQFSEIKPVLAQGLILATLGVLLT
                     AIFTGLIIWWILDLTIPGANIGLLTSLLLAATMSSTDSASVFSILRSKGLNLKKNLRP
                     MLELESGGNDPMAYVLVITLIDIIKMNSEPNFWLASGMIVLQLIIGGGVGYLLGKLAI
                     ITINHIKLGNDSLYPILVFTFCIFIFSITYFLRGNGFLAVYVGGLVIGNAKFVHKRSS
                     LNFFDGLAWLFQLVMFLTLGLLVNPHELIPIILPALAVSIIIIFITRPISVFICLLPF
                     RKLSFKDKSFVSWVGLRGAVPIIFAIYPLVENVPHARIIFNIVFFCTLISLLVQGTSI
                     PLVAKWIGVIEKPERFSKLKPFNMDFADNLKTVSTEIEITDKMLLRGNQIMNLELPDN
                     TLVVMVKRGDNYFLPSGSTTLKEKDKLLIITDNQEALVETYRNLGIDG"
     gene            4323..4526
                     /locus_tag="PK502_08725"
     CDS             4323..4526
                     /locus_tag="PK502_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00939.1"
                     /translation="MSSKDIRVIFRLHRKVWYAWCLSTFPGVELKINRVAINTAGSYQ
                     NPLYLKKSIFSTLSGDVVQMFYF"
     gene            complement(4628..5929)
                     /locus_tag="PK502_08730"
     CDS             complement(4628..5929)
                     /locus_tag="PK502_08730"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPH00940.1"
                     /translation="MNNKRLQPYAVYSDGKGNVYEDRSLFAVGRSGHEFYPLYLDEMI
                     PLPEGSDLFELPGRKTVGYSRNGKLTARQSGLAAAAFIAPAHTQLSVAAWENKVNAPV
                     LPLFAYTAVGWYANRFYVPAIRIDLDTRQDCENFDQQVVVERGKALLEQHEGNRLVNH
                     LVENCAFTYLCPAARNMVMERWEAPIPVSPTCNARCIGCISKQDQQESPIPETQHRLT
                     FVPTVGEIAGYAVPHLENAPNAVVSFGQGCEGEPLMQWELIRDAIKLIRSKTQKGIVN
                     LNTNGSKPRAVEELFKAGLDSIRVSMNSAQPKWYESYFRPVGFSQNDAIESLTIASRY
                     NRWASINYFVMPGFTDTAEELTALKNLIEKTDLNMIQWRNLNIDPDWLFGKTNFTGNS
                     KPLGIRYVMDEIHRSYPSVYFGYFNPHYEVMHRWQQQTGNS"
     gene            complement(6285..6503)
                     /locus_tag="PK502_08735"
                     /pseudo
     CDS             complement(6285..6503)
                     /locus_tag="PK502_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
     gene            6813..>7354
                     /locus_tag="PK502_08740"
     CDS             6813..>7354
                     /locus_tag="PK502_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPH00941.1"
                     /translation="MENESKSKLVIGILTGGGDVPGLNPAIRAITIRALREGYKVIGF
                     RRGWAGLIEVIRENDADNSKCYEVLTEERVNKAFRTGGTFLHTSRTNPSKVSKDSVPD
                     NLKSKYHKEVNDLTPEVLKNLAFLGIDYLIPIGGDDTLSYGVRLYQEGFKVIAIPKTM
                     DNDVPGTDYCIGFSTCITRT"
BASE COUNT         1946 a         1624 c         1668 g         2116 t
ORIGIN      
        1 gccatccaca tattcacctc aaccgtatcg ccctaccata aacccgagga tgatgaatat
       61 gtggatggcc ggaatacctt ggttaccaaa aggtgccgtg tcggtgtttg gcacgacatt
      121 cttaccgtcc ttttttaccg ttaaaccatg ttttgaggcc aggttgtgga gcatgacatc
      181 cccattctca agcgttttat ccccattcag gtctatacct ccatatcggg aaagcattcc
      241 taccatatca aggctgaaca tgacctttac ctttttgggt ggtacctgat tatgctgtac
      301 cataaaactc gatccaacca atccggcctc ctctccgtca aaggcagcta aaattaccga
      361 tcgtttcagc tggttcctgc ttccggcaag ccatcttcca atttcaatta tagctgctac
      421 gcccgaggcg ttgtcatcgg ctccgttgaa gactattctt ttaccatcgt ctgttatttt
      481 gtaggccaga tggtcgtagt gggctccaat cacaatgtac tcatctttaa gcagcgggtc
      541 tgtgccctca acccagccaa taatattagc cccttcggtt tttaccatta ttcccgagct
      601 gatgaaagga tgcaggtagc tcccctgcca gggcataact ccggcagcct cgagctgcgc
      661 tgtgatgtaa tcggctgcca tttttcctcc ggttgttcca aaacccctcc ccattaacga
      721 atcggaagca agggtgtaaa catgctgttt aattatcacc gaatcaaact cctgagcggc
      781 agcgttaagg gtgcagccta ctgccattaa ggctaaaatc aattttgatt tcatcttggt
      841 ttggtagctt agggttagag ctaaaaagtt tgatgttaag acggtaatgg tgcatttttg
      901 ttacagcata cctgcaccac atcctggtgt aaatgtaggt gattaatgta attttaacaa
      961 catgcttcgg ctatgcgcag tctatttttt cccctttgcc ttgccgttga cctttggctt
     1021 atcgtcaggg gcaaaaagat tttcacgtga tagcaggtgg tcgagatttg ctacgccata
     1081 cacaactatt gctgttacaa ttgaagtgtg gttcatgtag tcggcagggc atttttcgta
     1141 ggtgtcgcgt tcggtgtgcc aaatttcctg gtagctgaag tcgagcccat caacatcttc
     1201 cgttccaaat tggaaggcag gcactccggc catcaggaat ggagcggtat ctgttcctcc
     1261 tggattttta ggctttgggc gaggtgttgc ttccttgagg gtgaatggga agctaggatt
     1321 aatgctgttc aatgggctac aaattccgga aaagtcgttg agcatggctt ttggaaccga
     1381 aaggttgctt gccacggttg gtccgccatc acggttgaac atggctgaaa tggactgaag
     1441 cttatcgctg tttttgatta cccagctggt tgaacccagc aggccaaact cttcgcctgc
     1501 ccagaggcaa accaggatgg tgcgtttggg cttacctcct gctttcatga ttagccttgc
     1561 ggcttccatg gcaggggcta cccctgaagc gttatcggct gcgccggtgg ccacatcaaa
     1621 agcatccaga tgaccgccca ttataacata ctcgttaggg aattcggttc ccttaataac
     1681 cccgacaacg gtgtggtagg ggatagggcc tgggcgaaaa tggttgcgga tatcaaattc
     1741 cagctggaag tactggcgtt ccctggccat ccgctcaata attttatact gattttcatc
     1801 cagcttgatg tctggaactt ggggcaagct atcccaaacc atgctgttaa tattctttct
     1861 gtcgtaaagc accctgattg gattaggagc agattggatt attccgagta ttcctgcctc
     1921 taccatttgc ctgtaaaata aacccggttc ttcggtaaga ggaatcagct ctttgggtgg
     1981 ttggtttgcg ctacggttca tccagttttc ccgctgaatc tcatcgtttt tcttctcgat
     2041 ctcgttgttg ctggcaatta tgctatcgcg cctatggttt gccctaaccg agtagtctat
     2101 cggccaaccg ttgctttcgc ctgaaatcag cacccatgca ccttttagct tccctttcat
     2161 cctttcaaac tcacgttggg ttttaggttc agctacaaca tgtccgcgtt gaacaccctt
     2221 tgtccccgca gtgtaggatg gagtagcgaa atgaagtgtc atggagtggt ctccgagcag
     2281 cctgccaaac cagggcccgc ggttaaaccc tacaggaagc tcgtctactt cgtcgaggaa
     2341 aacatccata ccccatgcct gaaattgtcg ggcagcccaa acaacggcat cctcatacgc
     2401 tgaagaccca accggtcttc cgcctatttg gttgcatagg acgtcaaggt gcttcatggt
     2461 ttggttttca gcagttccaa tttggatgat tttctgtata accgaatcac tctgggcatg
     2521 ggaatgagcc atcaatcctg ctaaaacaaa aagcaatgta attattcgtt tcatggcaac
     2581 aacattttat tttcacatta gaattttttg ctccctgata gtttaacgag cggctattcc
     2641 atttatttac ttaattgccg atttaccact gcttctccag gatagatatt cggaaaatca
     2701 ggtagcacta tttaccgcga ttggctatgc cattatacgg gtttaggagc tcgggttgct
     2761 ggtgattttt tacccatcaa tacccaaatt tctatacgtt tcaacaaggg cttcttgatt
     2821 atccgtaata atcaaaagct tatctttttc ctttaatgtt gtactgcctg aaggaagaaa
     2881 atagttgtct cctcgtttca ccattacaac cagcgtgtta tcgggaagtt caaggttcat
     2941 tatctgattt ccgcgcagga gcattttgtc cgtaatttca atctcggttg aaacggtttt
     3001 caggttatcg gcaaagtcca tgttgaatgg ttttagcttg ctaaaccttt ctggtttttc
     3061 gataacgcct atccacttgg caactagtgg aatggatgta ccctgaacca gtagcgaaat
     3121 cagagtacag aagaatacga tgttgaatat aattcgggca tggggtacat tttcaactaa
     3181 agggtatatg gcaaaaataa tgggaacggc tcctcggagt cctacccacg acacaaatga
     3241 tttatccttg aaggacaatt ttcggaatgg cagcaggcaa ataaatacgg aaattggccg
     3301 ggtgatgaaa attatgatga tgcttacggc taaagctggt aaaatgattg gaataagctc
     3361 atgcggattt accagcaatc ccagggtaag aaacatgaca agttggaata gccatgccaa
     3421 cccgtcgaag aaatttagag aagaacgctt gtgaacgaat ttggcgtttc caattaccag
     3481 ccccccaacg taaacggcaa ggaacccgtt gccacgaagg aaataggtta tcgaaaagat
     3541 aaatatgcaa aaggtaaaaa ctaaaattgg gtatagtgag tcattaccca atttgatatg
     3601 gttaattgtg ataattgcca gcttacccag caaatatccc acgccgccac caataatcag
     3661 ctgcaaaact atcatacctg aagccagcca aaaatttggc tcggagttca ttttgattat
     3721 atctatcagg gtaattacca gaacataagc catgggatcg ttcccaccac tttccaactc
     3781 cagcatgggc cggaggtttt tcttaaggtt gagccctttt gaacgcagaa tggagaaaac
     3841 agaagcggag tcagtcgatg acattgttgc tgctagcagg agagaggtta gtagtccaat
     3901 gtttgccccg ggtatggtta aatcgagtat ccaccaaata attaacccag taaatattgc
     3961 tgttaacagt actccaagtg tggcaagtat taatccctgg gcaagaaccg gttttatttc
     4021 agaaaattgc gtgtccaatc ctcctgagaa tagaatgacg catagcgcta ttgtccctat
     4081 tgcttgagag atgtggatat tttcaaattg aatacccaac ccgtcgctgc cgctaagcat
     4141 cccaactatt aaaaataaaa gcagggctgg aactccgtat tttgagctaa cctttccagc
     4201 aagaatactg agaaaaaata gtgtggatag aataagtaat gccagttcaa gttgaatgct
     4261 tcccattttg ttattttaac ccataaagat agcatttttg agcaactttt acacagtgta
     4321 agatgagcag caaagatatc agggttattt tcaggctgca caggaaagta tggtatgcct
     4381 ggtgcttgag cacattcccc ggagttgagt tgaagattaa cagggttgcc ataaatactg
     4441 caggttcata ccaaaacccg ttatacttga agaagtcgat ttttagtacc ctttccggtg
     4501 atgtggttca aatgttttac ttctagtaaa tagaattact taaaacttca atacaaaaat
     4561 tacccatact cagaagtact actaaaatca atcatcccat taacttaaaa gtttacctgc
     4621 attgttctta actattacca gtttgctgtt gccaccgatg catgacctcg taatgggggt
     4681 tgaagtatcc gaagtaaacc gaggggtagc tacgatggat ttcatccatt acatagcgaa
     4741 ttccaagggg tttgctgttg cctgtaaagt tggttttacc aaacagccag tcggggtcaa
     4801 tgtttaagtt gcgccattga atcatgttca gatccgtttt ttcaatcagg tttttcaggg
     4861 cggttagctc ttctgcggta tcggtaaagc caggcattac aaagtagttg atggatgccc
     4921 aacggttgta ccggctggca attgttagcg attcaatggc atcattctgg ctaaagccaa
     4981 caggacggaa gtagctttcg taccatttgg gctgagctga gttcatgctt acccgtatgc
     5041 tatccagccc tgctttgaaa agctcttcta ctgcccgggg tttgctgccg ttggtgttga
     5101 ggttcacaat gcctttttgg gttttgcttc ttatgagctt aatcgcatcg cgtattagct
     5161 cccactgcat cagcggttcg ccttcgcaac cctgaccaaa gctcacaacg gcgtttgggg
     5221 cattttccaa atggggaaca gcatagcccg caatttcgcc aacggttgga acaaaggtga
     5281 gcctgtgctg tgtttcggga ataggcgatt cctgttggtc ctgctttgat atgcagccaa
     5341 tgcaccgggc gttgcaggtg ggagataccg gaattggtgc ctcccaacgt tccatcacca
     5401 tgttgcgtgc agcaggacag agataggtga aagcgcagtt ttctaccagg tgattcacca
     5461 gccggtttcc ctcgtgttgc tcaagcaaag ccttacccct ttcaaccact acttgctggt
     5521 cgaagttttc acaatcctga cgggtatcga ggtcgatccg aatggcggga acgtaaaacc
     5581 tgtttgcata ccagccaact gccgtatagg caaatagcgg tagtacaggt gcattaacct
     5641 tgttttccca tgcggccaca gaaagctgag tgtgtgctgg cgctataaaa gctgccgcgg
     5701 ccaatccact ttgcctggct gttagcttgc cgttacggct atatccgacc gttttacgac
     5761 ctgggagttc aaacaaatca ctaccctctg gaagaggtat catttcgtcg aggtagagag
     5821 ggtagaactc atggccagag cgtcctactg caaataggct cctatcttca taaacatttc
     5881 ccttcccatc actgtaaaca gcgtagggtt gaagtcgctt gttgttcatt ttctttattt
     5941 attgcctgta aaaatacccc tcttttcgca agccgtaaaa ttttggttaa aaaatcattt
     6001 atttatgctt ccatgcttta aggtaagtaa cttcctcaaa caaaagcttt caacacatac
     6061 tttgttcagc tgtagctgag agtgtttgtg cttgttgctt aatgcattat gattattgta
     6121 tgataaaaat gatgtgaagg caaattgcga gctttgtgga acggctaagg atcaatttta
     6181 tgccataatg taagcctgat tatctatcat aaataaaaaa ggccggttat atgccgacct
     6241 tttttattag aactatgttt agttgagttt ccttggagct tctcctagtc gttattgttt
     6301 agaaacccaa ggtagaagaa aagggttgcc aacgagccta aggcggcaac aacatacgtg
     6361 agggctgccc attttagtgc atcactggcg taaactacat tttgaccgta ctgaattccg
     6421 gttaagccta gccactggag cgccctacgc gatgcatcga actcaacagg caaagtaatc
     6481 aggctgaaaa gggaggtgtg agagggcggg gaataatcac cctacatact caattattta
     6541 taagttattg atattcaatc tatagtaggt tatgccgtaa tatctggcta attaacatta
     6601 gggatgggta tgaccggtgc taaccgtttt ggtaattttt tcttgtggag aagcatttgt
     6661 agttgaatat actaccatcc cgattatttt ggaaatgggt ttatgttgtg ggtggtgaaa
     6721 atttaacatt ggtaaaccaa tgaaatttat gatataaaac atatatttgc taggtatttg
     6781 ataattgacc cgtcataaac caattgaaaa tcatggaaaa cgaatcaaaa tctaagctgg
     6841 tcattggaat tcttactggg ggaggagacg tcccgggatt aaatcccgcc attcgtgcca
     6901 tcactatcag ggcgctacgt gaaggatata aggtaatagg cttccgcagg ggctgggcag
     6961 gattaataga ggttatccgt gaaaatgatg ccgacaacag caagtgctat gaagtgctaa
     7021 ccgaggagcg ggttaacaaa gcttttagaa cggggggaac ttttctacat acctcgcgga
     7081 caaacccaag caaggtttcc aaggatagtg ttcctgataa cctgaaaagc aagtaccaca
     7141 aggaggtaaa tgatcttact cccgaggttc tgaaaaacct ggccttttta gggattgact
     7201 accttatccc tattggagga gacgataccc ttagctatgg agttcggcta taccaggagg
     7261 gttttaaggt aattgccatt ccgaaaacaa tggataacga tgtgcctggt accgattact
     7321 gcataggttt tagcacctgc ataacccgta ccat
//
LOCUS       DAOPHX010000120         7306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_13014_length_7306_cov_7.125776, whole genome shotgun sequence.
ACCESSION   DAOPHX010000120 DAOPHX010000000
VERSION     DAOPHX010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13014_length_7306_cov_7.125776"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PK502_08745"
     CDS             complement(<1..233)
                     /locus_tag="PK502_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00942.1"
                     /translation="MLCELKNRCVKSSGRGFGVRLPHDSNRSASWRRARRRQPYGSDG
                     ASRSEYLLEADPLRFIRLIPGHKSGQVTDAYTHA"
     gene            complement(396..1049)
                     /locus_tag="PK502_08750"
     CDS             complement(396..1049)
                     /locus_tag="PK502_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HPH00943.1"
                     /translation="MTKPRVKICCIGSIEEAKIAIEHGASALGLVGKMPSGPGVIGDE
                     LINKIARAVPPPISTFLLTSETKPKNIISHYKKVYTSTIQIVDELEDRGYELIRNELP
                     NVKLVQVIHVIDDNSVKEAIEISKYVDAILLDSGNPNLPVKKLGGTGRTHNWELSLKI
                     RESIPIPIFLAGGLNKDNVRQAIETVHPFGIDLCSGVRTNGKLDKNKLREFFNAVNE"
     gene            complement(1161..2042)
                     /gene="lgt"
                     /locus_tag="PK502_08755"
     CDS             complement(1161..2042)
                     /gene="lgt"
                     /locus_tag="PK502_08755"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPH00944.1"
                     /translation="MIVNYIHWNIDPEIINIFDISIRYYGILFVGGLVLCMLILKGIF
                     KKEGIPLNKLEKLSIYGIIGIFAGARLAHCLFYEPSYYLSHPLEIILPIQPTVDGGYK
                     FTGYQGLASHGGALGLIIALIIYARKTKESIIKTIDLIAIVAGLGACFIRLANLMNSE
                     IIGIPTNVPWAFIFERVDSLPRHPAQLYEAIAYLLIFGLMLYLYKTKRENLKNGFFFG
                     LVITLIFVSRFFIEFIKEKQVPFEEQMKLDMGQLLSIPFIVLGTGFIIHGLVKTKKIG
                     PAHNGKAIACAAPIPRL"
     gene            complement(2976..3051)
                     /locus_tag="PK502_08760"
     tRNA            complement(2976..3051)
                     /locus_tag="PK502_08760"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3014..3016),aa:His,seq:gtg)
     gene            3187..4488
                     /locus_tag="PK502_08765"
     CDS             3187..4488
                     /locus_tag="PK502_08765"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HPH00945.1"
                     /translation="MKLDNTRALQSYQIIRFLAVFTIGMAMVKLSLPQAQVGQYEVVL
                     FIASLLTTFWITGIIQALLPMTSSSVSRDSGSKRPEIFCALVVVVALTLVSALLLLVF
                     KNLIGQLAGVANFPYFKQTILYIMLSTPTLLIEYIYLIENQPRKMIAYGLIAYSLQVA
                     MVVLPLLVDVRVELCIFGLIGISAIRMVWLLVVVRRYADLKIDRKFMFDFIAKSTPLS
                     LKYLVSSSGLYIDQLIISAYYDSSTFAAYRFGAREIPLITILTVGLSNSMLVEFGVKE
                     HIGDTLERLRRESLKLMHILFPLSVVAIILSKPLFPVIFSSDYLQSANIFMLYALLVT
                     SRTIFPQTVTMGFQRNRFALLASTLEMVLNIALSLVFIQLFGIEGVALATVVVYLFEK
                     VLLAGYNGRRLGIHPAEYIPMKPYTAYCTLTIATYLLVRFV"
     gene            complement(4561..6288)
                     /gene="lysS"
                     /locus_tag="PK502_08770"
     CDS             complement(4561..6288)
                     /gene="lysS"
                     /locus_tag="PK502_08770"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPH00946.1"
                     /translation="MALLDELSEQEVIRRQSLDELRKLGIDPYPAAMYPVTHTTGSIL
                     SNFENLCEQQAEVCIAGRIMARRIMGSASFLELQDEKGKIQVYVKRDDICPGEDKTFY
                     NVVFKKHLDIGDIIGVKGFIFRTQMGEESVHAREITLLSKSIRPLPIVKEKDGKVFDA
                     VTDAELRYRQRYVDLIVNPQVREVFVKRTKIVNTMRDFFNSKGYLEVETPILQPIPGG
                     AAARPFITHHNTLDMPLYLRIADELYLKRLIVGGFNGVYEFSRDFRNEGMDRTHNPEF
                     TVLEIYVAYKDYFWMMDFTEEMLERVALSLHGTTAITVGDSTINFKRPFRRLTMTDAI
                     KEYAGVDITGKTEEQLRSICKEQGIEVDETMGKGKLIDTLFGEKCEQHLVQPTFIMDY
                     PIEMSPLCKRHRSNPELTERFELIVGGKELCNAYSELNDPIDQLERFQEQLRLSEKGD
                     DEAMFIDMDFVRALEYGMPTCSGMGIGIDRLTMFMTNQPSIQDVILFPQMRPEKKQLK
                     DPVEAYSQRNIPAEWVPVLQKLGYSTVVSLTNVAPGKLFNDICGYNKKNKLGLQNPSM
                     DDVKRWVEQ"
     gene            6849..7235
                     /locus_tag="PK502_08775"
     CDS             6849..7235
                     /locus_tag="PK502_08775"
                     /inference="COORDINATES: protein motif:HMM:NF025652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4372 domain-containing protein"
                     /protein_id="HPH00947.1"
                     /translation="MGKDTAKNLDGQPVFKQVMKILPGEQFDIVVRQCGSDCCYKSFF
                     SWDQLVVMLFGIFSRCDSMGEVCDGMRALGGKLNYLGMDSAPSKSTAGDALRDRNNEV
                     FMRYYFALIKYVEAAFVGQPKGEDQL"
BASE COUNT         1914 a         1632 c         1550 g         2210 t
ORIGIN      
        1 gcatgggtgt atgcatctgt aacttgacca cttttatggc ctggtatcaa ccggataaac
       61 cgcagggggt cagcttcgag caggtattcg gaacggctgg ccccatcgct gccgtacggt
      121 tgccttcggc gagctctccg ccagctggcg gatcggttac tgtcgtgtgg cagcctcacc
      181 ccaaaccctc tgccactaga ctttacacat ctatttttta attcacaaag cacaatacca
      241 ccagtttacc tcttttttct accacctgtt tccttctttt tctatgcttc actaccccac
      301 gagaggactc gcgcagtagc ggggcagagt agaatacctg atttaaccac ttaaaatcca
      361 gaaatttata ttgctggtaa cgccttattt ttttgctatt cattaaccgc attaaaaaat
      421 tctctcagct tatttttgtc aagttttccg ttagtacgaa caccgctgca gaggtcaatg
      481 ccaaacggat gaactgtttc aattgcctgt cttacattat ctttatttaa ccctcctgca
      541 aggaaaattg gaattggtat tgattccctg atttttaggc ttaattccca attatgtgtt
      601 ctccctgttc cgcctagctt ttttactggt aaatttggat ttccgctgtc caataaaatt
      661 gcatcaacat acttggaaat ttcaatggct tcttttacag aattatcgtc aataacatga
      721 ataacttgca ctagttttac attcggtaac tcgttacgaa tcagctcata acctctatct
      781 tccagctcat ctacaatttg aattgttgat gtgtacactt ttttgtaatg cgagataatg
      841 ttttttggct tagtttcgct tgtcagcaag aaagtagaaa tcggtggagg aacagccctt
      901 gcaattttat ttattagctc atcaccaatt acacccggtc cacttggcat tttcccgact
      961 aagcccaaag cagatgcacc gtgttcaatt gcgatttttg cttcttcaat gctacctata
     1021 cagcagattt ttactctagg ttttgtcatg gcgtgccgtt taagtcgttt ttttaagggt
     1081 gatgtagaac aaatggtggc atgattagtt gccgaattcg aagcaattcc ggatcaagta
     1141 ataccaaact ttgataagag ctataacctt gggattggcg ctgcacaggc aatagcttta
     1201 ccgttgtgcg ctggtccgat tttttttgtc tttaccaatc catgaattat aaatccagta
     1261 cctaaaacaa taaacggaat actcaacaat tgtcccatat ctaatttcat ctgttcttca
     1321 aaaggaactt gcttttcctt tataaattcg ataaagaaac gggatacaaa aatcaacgtg
     1381 attaccaacc caaaaaagaa tccgttttta aggttttccc gtttagtttt ataaagatat
     1441 agcatcaatc cgaatatcag taaataagca attgcttcgt atagctgagc cggatgtcgt
     1501 ggcaaactat ctacccgctc aaaaataaaa gcccacggaa catttgttgg aattccaata
     1561 atctcagaat tcatcaagtt ggcaagtctt ataaagcatg ctcctaaacc tgcaacaatt
     1621 gctattaaat caatcgtttt tataattgat tcttttgtct ttctggcata aattatcaaa
     1681 gcaataatta aacccaatgc tcctccatga ctggctaacc cttgataacc agtgaattta
     1741 tatccgccat caacagtcgg ttgaattggt agaataattt ctaatggatg gctcaaatag
     1801 tatgatggct cataaaataa gcaatgagca agtctagctc cggcaaaaat tcctattatt
     1861 ccgtaaattg aaagtttttc taatttattc aacgggattc cctctttttt aaaaatacct
     1921 tttaagatta acatgcaaag caccaatcct cccacaaata ggatcccata gtatctaatc
     1981 gaaatatcaa aaatattgat aatctcaggg tctatattcc aatgaatata atttactatc
     2041 ataatttttc tttgtcttgt tgggtctgct aatcacgtaa ccaaggttcg aagttaacaa
     2101 aagttgtgaa aatcgttaac aaaatttttt atttgagtaa aaggcaactt gcttacacct
     2161 tatccttact ccctttaaac acttattttt gttagcggtt gttgttcttt ttatcaacat
     2221 atttgaaatt tttatgtcca tcttgaacct caatcagttc aatgtttttt attcgtattt
     2281 gttcggtata ataaataaat ctcgaaccat ctccttttag aatagaatcc tgtacgaaca
     2341 tttgctcaaa atagaaataa attcttttgt tatttatacc agtaacgcga acttaaattg
     2401 ttgggctact ttttaattca aatggactat tatctttatc tacacatttt atgtagtaaa
     2461 aaggatatgt tttataagta acaacatctc ccattaaacc tctagcatct gcggttttta
     2521 attcaaccga atcaatggct ttaaattgtt cattaaaact tttcaatcgt gatataatat
     2581 gttttacagc tcgaaattat cattgtgaaa accataatga aaatcacatt aaagccctta
     2641 tgtctcataa tgcggtaaaa tttttattaa tacatcgttt gtcgtaatta aaccctattg
     2701 attcgtacat ctatcttgct gtatgtgtgt tttttataat aaccactaac tcactcgtat
     2761 gtaataccat tcattttggg tttaagaatg atgcaggcat cacatgcact caacaaataa
     2821 atgattattt tccttacttt tcgcctgcca taattctgtg caggaccata aggacgcaaa
     2881 cggtttatcc atgcagcatg ggggggcgct gcatttccgg gcatggagtg ggtgtggaat
     2941 ggagcaaaaa aaaaccggag ttgcctccgg ttcaggggtg gaagatggga cttgaaccca
     3001 cggcccccag atccacaatc tggtgctcta accagctgag ctacatccac catgtgattt
     3061 aggctgcaaa gttataaaat ttcttcaacc tgaaataaaa aaatggggaa actttgttgc
     3121 ccatgtcctg gctgcgagct gtttttttaa tactttagca tcctgtagcc atttttgcta
     3181 cgtaaggtga agctagacaa cacaagagcc cttcagtcct accagattat caggtttctg
     3241 gctgttttca ccattggtat ggccatggtg aagctatcgc ttcctcaggc gcaggttggg
     3301 cagtatgagg tggtgctgtt tattgccagc ctccttacca ccttttggat taccggaatt
     3361 attcaagccc ttctccccat gacgtcttca tctgtcagca gggattcggg ttccaagcgt
     3421 cctgaaattt tctgcgctct ggtagtggtt gtggcgttaa cccttgtatc ggctttgctg
     3481 ctgctggttt ttaaaaacct gatcgggcag ctggcagggg tggccaactt cccttacttc
     3541 aagcaaacaa tcctgtacat catgctgagc accccaacgc tcctgattga gtacatctac
     3601 ctgattgaaa atcaacctcg taaaatgatt gcctatgggc ttatagccta ctcgttgcag
     3661 gtggccatgg tggtgttgcc cctgctggtt gatgtcaggg ttgagctttg cattttcggt
     3721 ttgataggca tttcggccat ccggatggtg tggctgttgg ttgttgttcg ccggtatgcg
     3781 gacctaaaaa ttgaccggaa gttcatgttt gattttattg caaagagcac gccgctatcg
     3841 ttgaagtacc tggttagcag ctcgggcctc tacattgacc agcttattat ttcagcctac
     3901 tacgattcgt caacctttgc agcttaccgg tttggcgcca gggaaattcc gctaattacc
     3961 atactcaccg ttgggctgag caactccatg ctggtagagt ttggggtaaa ggagcacatt
     4021 ggcgataccc tggagaggct caggagggaa tcgctgaagc tcatgcacat cttgttcccc
     4081 ctgtcggtgg tggcgattat cttaagcaag cccctattcc cggtgatatt tagctccgac
     4141 tacttgcaaa gcgccaatat tttcatgctt tatgccctgc tggttaccag cagaactatt
     4201 ttcccgcaaa cggttaccat gggatttcag cgaaatcgtt ttgcgctgtt ggcctctacg
     4261 ctcgagatgg tgctaaacat agcattaagc ctagtattta tacaattatt tggaattgaa
     4321 ggggttgcgc tggctacagt tgtggtttac ctttttgaaa aggtgctgct tgccggatat
     4381 aatgggcgta ggttgggtat tcatcctgca gagtacatac caatgaaacc ctataccgcc
     4441 tactgtacgc ttaccattgc cacctacctt ttggtacggt ttgtatagcg gcataacgct
     4501 acactttcct gccatttatg aagcaacctg cgaatttcac cgggctatga attcatacac
     4561 ctactgctca acccagcgtt taacatcgtc catacttgga ttttgcagcc ccagcttgtt
     4621 tttcttgtta tagccgcaaa tgtcgttaaa cagcttaccc ggagcaacat tggttagcga
     4681 tacaacggtg ctgtagccca acttctggag aacaggcacc cactcagccg gtatgttcct
     4741 ttgggaatag gcctcaacag ggtctttcag ctgttttttt tcagggcgca tctgagggaa
     4801 taggatcaca tcctgaatgg agggctggtt ggtcataaac atggttaacc ggtcaatccc
     4861 aattcccatt cccgagcagg taggcatacc atattccagg gcacgaacaa agtccatatc
     4921 aataaacatg gcctcgtcgt cgcccttttc gctgaggcgg agctgttcct gaaatcgctc
     4981 aagctggtca atggggtcgt tgagttcgct gtaggcgttg caaagttcct tgccgcctac
     5041 aatcagctca aaacgttcgg ttagctcggg gttgctacgg tggcgtttac acaatggcga
     5101 catctcaatt gggtaatcca tgataaacgt tggctgaacc aggtgctgct cgcacttttc
     5161 accaaaaagg gtgtcaatca gcttgccttt gcccatggtt tcgtccacct caataccctg
     5221 ctccttgcat atgcttcgga gttgttcctc ggtttttccc gtgatatcaa cgccggcgta
     5281 ctccttgatg gcgtcggtca tggtaagccg gcggaagggt cgcttgaagt tgatggtgct
     5341 gtcgccaacg gttatggctg ttgttccatg cagggataag gcaacccgct caagcatctc
     5401 ctcggtgaag tccatcatcc aaaagtagtc cttgtaggcc acgtaaattt ccagcacggt
     5461 gaactcaggg ttatgggtgc ggtccatccc ctcgttgcga aaatccctgg agaactcgta
     5521 aacgccgtta aacccaccaa caatcaggcg tttaaggtat aattcgtcgg caatgcggag
     5581 gtaaagtggc atgtcgagtg tattgtggtg ggttataaac ggccgggcag ctgcacctcc
     5641 cggaataggc tgtaatatag gggtttcaac ctcaagatat ccctttgagt taaagaaatc
     5701 gcgcatggtg ttaactattt tggttcgttt aacaaacacc tcgcgcacct gagggttaac
     5761 gataaggtca acatagcgct gacggtacct gagttcagcg tcggtaaccg catcgaacac
     5821 cttgccatcc ttctccttga caatgggtag aggcctgatg gactttgaaa ggagggtgat
     5881 ttcccttgcg tgaacagact cctcacccat ctgggtacgg aaaataaatc cctttacccc
     5941 gatgatatcg ccaatatcca gatgtttctt gaatacgacg ttgtagaagg ttttatcttc
     6001 tcctgggcaa atatcgtcac gtttaacgta aacctgaatt ttcccctttt cgtcctgaag
     6061 ttcaaggaac gaagcgctgc ccataattct gcgtgccatg attcggcccg caatgcaaac
     6121 ctcagcttgt tgttcgcaaa ggttttcgaa gttggaaagt atgctgccgg ttgtgtgggt
     6181 aaccggatac atggcagcag ggtaggggtc tattcccagc ttccgcagct catctagtga
     6241 ttgcctacga attacctcct gctcgctcag ctcgtcaagt aaagccatac taataggtga
     6301 ttaggtaaaa accgtgcaaa tttacccgaa ttttttataa ccagaagaaa atgtaccgaa
     6361 tcaatgatca acaagccgga ttaaaacagt gctaacggat ttactcgtta cagcagtttc
     6421 taccacgtca gggggtcagg acgcggcaat gctggatact tacgctttcg ggtaaaggat
     6481 ggtggcaaaa gcaaggcact ccccaagccc atgaggctac gggaactagc tggttcaaag
     6541 aacttggttt atacagatta tcatttcaca ttacactaca aagcattgca ccattaaagg
     6601 aaaccgccgt gtccgccgca ggcggatgca cggtggtgta agggggcgag gagtaatccc
     6661 ccctacctac tcgatttaaa atcctatatt cctttgttcc tgaaaacctg gacttttatt
     6721 cggttctaaa cctaaaattt tagttttaag tctctactga ccggttaaaa tttcagtgaa
     6781 aaagggacta ctcccgcgag gtttcgcccc gatgttgtag gcttgttttg ctaaaaaaaa
     6841 actgcaacat gggcaaagat acagcaaaga atttagacgg tcagccggtt tttaaacagg
     6901 ttatgaaaat tcttccggga gagcaatttg atattgtagt gaggcagtgc ggcagcgact
     6961 gctgctacaa gtcctttttt tcatgggatc agctggtggt gatgctattc gggatttttt
     7021 cccgctgcga ttcgatgggc gaagtatgtg atgggatgcg ggctttggga ggaaagctca
     7081 actaccttgg gatggacagc gcaccgtcca aaagcacggc aggagacgct ttacgtgacc
     7141 ggaacaatga agttttcatg cggtactact ttgcgctgat caagtatgtt gaagccgctt
     7201 ttgtcggtca gccgaaagga gaagatcagc tttgagaagt tttatgcctt tgattcgacc
     7261 acgataacgc tgttttcaga cgtcatgaaa ggggttggcc gtaacc
//
LOCUS       DAOPHX010000121         7147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_13420_length_7147_cov_6.045685, whole genome shotgun sequence.
ACCESSION   DAOPHX010000121 DAOPHX010000000
VERSION     DAOPHX010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13420_length_7147_cov_6.045685"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..1667)
                     /locus_tag="PK502_08780"
     CDS             complement(279..1667)
                     /locus_tag="PK502_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPH00948.1"
                     /translation="MAYRFARLLFAGLFFALPLAGYPQLTAPGRSWAGVTQYVNSPLH
                     QDSIFVFFSNVGSLRASHSTGNNASFKWYRYDPLAPSISERFVLFSTPSDGFFSQLDN
                     LAEGGYRVEVTDDADSTETFTAWLFTDNVTLTGIDVFSQCSFLQLTAISSPDQYAVEY
                     DRFVYYDLSRTSHTVNNKYGKDYFKVISWNASEPRVEVPDIQQLKLTLENPAPLWNSA
                     YTVTITNCFGRQLPVASTDVVTAIAAKSDMKIQVDANGVWTDWSSNERYEALLGLRLE
                     SLSVNVDSLYWQVVNEKIGKFANEYFVIWRDSSTVEQRKEAFPDKDLMAPGYYLVKHF
                     VINTTSGCRDSMVVNIEVDSSQIKADALPNVFSPNDDGVNDVFSFINPEENIKSIRTF
                     TIRILSRTGKQIYSYSGDPREWKGWDGRIEGSGAKAATGVYYFIIEARGWDDRTFKRG
                     PYKGFLHLYADR"
     gene            2089..5532
                     /gene="ileS"
                     /locus_tag="PK502_08785"
     CDS             2089..5532
                     /gene="ileS"
                     /locus_tag="PK502_08785"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPH00949.1"
                     /translation="MSCSKFKEYKGLDLSQVNSEVLQAWDSNDTFHKSLETRSGHPRF
                     IFYEGPPSANGIPGIHHVMARTIKDVFCRYKTQKGFFVERKAGWDTHGLPVELGVEKM
                     LGITKEDIGTKISVEEYNQTCRREVMKYTDLWEDLTHKIGFWVDMKNPYITYDNRYIE
                     TLWYLLQQLFQKGLLYKGKTIQPYSPAAGTGLSTHELNQPGCYRDVKDTTVVAQFKLV
                     QNEQSSFLFNLTDSEIYILAWTTTPWTLPSNTALAVNPGIEYVLVKTFNPYSGSPMTA
                     ILAKDRMDYYFSPANSNLSFDSFNAGDKNIPFRVIGSFKGSQLVGLRYEQLFPWVKPD
                     GDAFRIIAGDFVTTDEGTGVVHIAPTFGADDDRAAKASGIAPLILRDRFNSVEPMVDR
                     KGKFYKLEELSPDFVKSNVNIELYSEFAGRYVKNDYDPTLTDDDTTLDVDLAVSLKRD
                     GKAFRIEKHIHSYPHCWRTDKPVLYYPLDSWFIKVTALKDRMIELNNTINWKPASTGS
                     GRFGKWLENLLDWNLSRSRFWGTPLPIWVTEDRSEMICIGSVAQLKAEIERSVKVGFM
                     KSNPLDNFVENDNSAENYEKFDLHRPFVDTIVLVSASGKKMFREPDLIDVWFDSGAMP
                     YAQLHYPFENGERFNELFPADFVAEGVDQTRGWFYTLHALAALLFDSVAFKNIISNGL
                     VLDKNGNKMSKRLGNAVDPWQVIEEYGSDPLRWYMLTNSQPWDNLKFDVAGVDEVRRK
                     FFGTLYNTYSFFALYANVDGFRYAEEEVPVQNRPEIDRWILSLLNSLVREVENAYEAY
                     EPTRAGRAIQDFVTEQLSNWYVRLNRKRFWGGDYSTDKVAAYQTLYTCLETVAMLASP
                     MAPFYMDQLFTDLNAVTGRHTDQSVHLTDFPTYSNELIDKTLEEKMEIAQTVCSLVLG
                     LRRKVNIKVRQPLSKIMIPVLDERFRVQLDSVKDLILSEVNVKELEYLSDSSGILVKK
                     VKPNFKSLGPRFGKSMKSIATALSELSQADINTFERSGKLTLNIPEGSIDLLLSDVEV
                     LSEDIPGWLVASEGRITVALDITITEALRLEGIARELINRVQNIRKDSGFEVTDKIRI
                     TLKKHDKLNPAVEAHYDYIAGQTLAREIILTESELNGNAIVLDLDDDLITSICVEKIN
                     G"
     gene            5583..5960
                     /locus_tag="PK502_08790"
     CDS             5583..5960
                     /locus_tag="PK502_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPH00950.1"
                     /translation="MGEKTRYSDEELAEFKELILQKLEKARNDYEILKSAITMSESND
                     TEDTSPTFKVLEEGASTLSKEEAGKLAQRQLKFIEHLEAALVRIENKTYGICRETGKL
                     ISKERLRAVPHATLSIDAKNNQR"
     gene            6046..6711
                     /locus_tag="PK502_08795"
     CDS             6046..6711
                     /locus_tag="PK502_08795"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HPH00951.1"
                     /translation="MRLTKQTTSALVVITVLLIDQLLKIWIKTHFAIGDEMRVFGNWF
                     ILHFTENPGMAFGMNFWGVWGKLILSIFRLAAIVLIGWYLNHVIKQGAKTGLVVGISL
                     VMAGAIGNMIDSAFYGLLFDSGTVYNHDMNGWVSYYGVSKLNFDGYAGFLRGCVVDML
                     YFPVINTVIPSWVPIWGGHQFQFFRPVFNIADSAITTGVIYLILFQRNNIIIKDKGKG
                     VKG"
     gene            complement(6930..>7147)
                     /locus_tag="PK502_08800"
     CDS             complement(6930..>7147)
                     /locus_tag="PK502_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00952.1"
                     /translation="TALWESRYLHRQHTLSRLFVSKLFRPFRACKKSFAPYKGLRPLL
                     LIPPLRGSCLNALQALLFKPISPFQGL"
BASE COUNT         1977 a         1624 c         1746 g         1800 t
ORIGIN      
        1 cccttgtatg gagcgaacga tttttacaag ccctgaaggg gcggaatagg tttgagacaa
       61 agagcctgga gagggtgtgt tggcgatgca agtagcggct ttttccaaag agctgtacgg
      121 attctttgca tcgcaacggg aagaaataag tataaagcgt aaagggtaaa aaaaattcca
      181 aaacttgttt gcctgttgaa gaattagccc ttaaccaggc tttggaatcg aaaaacatct
      241 ccaattttaa aagggtaaca gggaaaaacc atgaaaattt acctgtcggc gtagaggtgt
      301 agaaaaccct tgtaggggcc acgcttaaag gtgcggtcgt cccagcccct agcctcaata
      361 atgaagtagt aaacccccgt ggcagccttg gctccgctcc cctcaatgcg tccgtcccag
      421 cccttccact cgcgcgggtc gccactgtag gagtatatct gcttgccggt tcggctaagt
      481 attctaatgg taaacgtccg gattgacttg atgttctcct cggggttaat gaaggaaaac
      541 acgtcgttta ccccgtcatc attgggtgaa aatacattgg gcagggcatc ggcttttatc
      601 tgcgaggagt caacctcgat gttcaccacc atggagtcgc ggcatccgga agtggtgttg
      661 atcacaaaat gctttaccag gtagtagcct ggcgccatca agtccttatc gggaaaagct
      721 tcctttctct gctccaccgt ggagctatcg cgccaaataa cgaaatactc gttggcaaac
      781 ttgccaattt tttcgttaac cacctgccag taaagggagt cgacgttaac cgaaaggctc
      841 tccaggcgga gtcccaatag agcctcatac ctttcgttgc tgctccaatc ggtccacacg
      901 ccattggcat ccacctgaat tttcatgtcg gatttggcgg caatggccgt caccacatca
      961 gtagaagcaa ccggtagctg ccttccgaag cagtttgtaa tggttaccgt gtaagctgaa
     1021 ttccaaaggg gggcaggatt ctcgagggta agcttaagct gctggatatc gggaacctcc
     1081 acccttggtt cggaggcatt ccacgaaatc accttaaagt agtcctttcc atacttattg
     1141 tttaccgtat gcgaggtacg cgaaaggtcg tagtacacaa acctgtcgta ctcaacggca
     1201 tactggtcgg ggctcgatat ggcagttagc tgcaggaagc tgcactggct aaaaacgtca
     1261 atgccggtta gtgttacatt atcagtaaaa agccatgctg taaaagtctc agttgaatcg
     1321 gcatcatcgg taacctcaac cctataacct ccctcggcta agttgtcgag ttgagaaaaa
     1381 aaaccatcgg agggagtgct gaagagcaca aatcgttcgc ttatggaagg cgccagaggg
     1441 tcataccggt accatttaaa tgaagcatta ttgcctgtag aatgggacgc cctaagcgag
     1501 ccaacatttg aaaaaaatac aaaaattgag tcctgatgca atggtgagtt cacatactgc
     1561 gtaacgcctg cccagctacg acctggagcg gtaagctgtg ggtaaccggc caggggtaat
     1621 gcaaaaaaca accccgcaaa aagtaacctt gcaaatcggt aagccattgt gcaaacgtaa
     1681 aattagctac aatattaacg aataaaaagt aaattagtaa tcccagtgcc tgaatcgtta
     1741 aatatgagcc ctatagtcga atcgtgaaaa ggtagagtag aaggttaaca gctttatact
     1801 tgcccggcac gtgtcagggg aacctgtctt aaacccgaca agcatccgaa gccttcagta
     1861 tcttttaagc cctatccttt gtcacaaatt gaagcattat gcgtattttt caaagctaca
     1921 ccacaaattc ccaacccgtt actacaacgt ataagctttc gattaatcat aatttcaata
     1981 actttgtagg atttttaaaa gggctttttt gtttataact aagtgttatt cagcaatata
     2041 agttgatttc ataagtattt ttttgcacaa taagtaatta gcaattagat gagttgttct
     2101 aaatttaagg agtacaaggg tttagacctt tcacaagtga atagtgaggt gctgcaggca
     2161 tgggactcta acgatacctt tcacaagagt ttggaaacca gaagtgggca tcctcgtttc
     2221 atcttttacg aagggcctcc atcggcaaac ggaattcccg gaattcacca cgttatggcc
     2281 cgaaccatta aggatgtatt ttgccgctat aaaactcaaa agggtttttt tgttgagcgt
     2341 aaggcaggct gggacaccca tgggcttccc gttgagctgg gggttgaaaa gatgctgggt
     2401 attaccaagg aggatattgg aacaaagata tcggtggagg agtataacca aacctgccgc
     2461 agggaggtaa tgaagtacac cgacttgtgg gaggacctta cccataaaat cggtttctgg
     2521 gttgatatga aaaaccccta tatcacctac gataaccgat atatagaaac cctttggtac
     2581 ctactccagc agctatttca gaaagggttg ctctacaagg ggaaaaccat tcaaccctac
     2641 tcgccggctg ccgggacagg gcttagcact catgagttga accagcccgg atgctaccgc
     2701 gacgtgaagg ataccactgt tgtagcccag tttaagctcg tccaaaatga gcaaagcagc
     2761 ttccttttca acctgaccga ttcggaaatt tacattctgg cgtggactac tacaccctgg
     2821 acactcccat caaacactgc tctggcggtt aaccccggta tagaatacgt actggttaaa
     2881 acatttaacc cctatagcgg aagcccaatg acggccattc tggctaaaga ccggatggac
     2941 tactacttct caccagccaa ctcaaacttg tcgttcgata gctttaacgc aggcgataag
     3001 aatatacctt tccgggtgat aggcagcttc aagggcagcc agctggtagg gttacgctat
     3061 gaacagcttt ttccatgggt taaacccgat ggcgatgcct tcaggataat agcaggtgat
     3121 tttgttacca ccgacgaggg aacgggcgtt gttcatattg cccccacttt tggtgccgac
     3181 gacgaccgtg cagcaaaagc ttcgggaatt gccccattaa ttcttcggga taggttcaat
     3241 agcgttgagc caatggttga ccgcaaaggc aaattttaca agctggagga gcttagcccc
     3301 gattttgtca aatcaaatgt aaacatcgag ctctatagcg aatttgccgg tcgttacgtt
     3361 aaaaacgatt acgaccctac ccttaccgat gatgatacta cgcttgatgt tgaccttgcc
     3421 gtttcgctga agcgcgatgg aaaggctttc cgcatcgaaa agcatattca tagctacccc
     3481 cattgctggc gcaccgacaa accggtgctt tactacccac ttgacagctg gtttattaag
     3541 gtaactgcgt taaaggatag gatgatagag ctgaataaca ctatcaactg gaagcctgcc
     3601 agcacgggaa gcggacggtt tggtaaatgg ctcgagaacc tgcttgattg gaacctttcg
     3661 cgctcccgtt tctggggaac tccactccca atttgggtta ccgaggatcg ttcggagatg
     3721 atctgcattg gttccgtggc gcagctcaaa gccgagattg aacgaagcgt taaggttgga
     3781 ttcatgaaaa gtaacccgtt ggataatttc gttgaaaacg acaatagcgc ggaaaactat
     3841 gaaaagttcg atcttcacag gccatttgtc gataccattg tgctggtttc ggctagcggc
     3901 aaaaagatgt ttcgcgaacc cgatctcata gacgtttggt tcgactccgg ggctatgccc
     3961 tacgcccagc ttcactaccc gttcgaaaat ggcgaaaggt tcaacgagct cttccctgcc
     4021 gattttgttg ccgaaggggt tgatcaaacc cgtgggtggt tctacaccct tcatgccctt
     4081 gccgccctgc tgttcgattc ggttgcattc aaaaatatca tctccaacgg gttggttctc
     4141 gataagaatg gtaacaagat gagcaagcgc cttggaaatg ccgttgaccc gtggcaggtt
     4201 attgaggagt acggctccga cccgctgcgc tggtacatgc tcaccaactc ccaaccctgg
     4261 gacaacctga aatttgacgt ggctggtgtt gacgaggtaa ggcgcaaatt ctttggcacc
     4321 ctctacaaca cctactcatt ttttgccctt tacgctaatg ttgacggatt tcgctacgcc
     4381 gaggaggaag tccccgttca aaatcgtccc gaaattgacc gctggatttt gtcgctccta
     4441 aactcgctgg ttagggaggt tgagaatgct tacgaagctt acgaacccac ccgtgcaggg
     4501 cgcgcaatac aggattttgt taccgagcag ctgagcaact ggtacgtaag gcttaaccgg
     4561 aagcgctttt ggggaggcga ttacagtacc gataaggtag cagcttacca aacactctat
     4621 acctgcctcg aaaccgtggc aatgcttgca tcaccaatgg ctccatttta tatggaccag
     4681 ctctttaccg atttaaacgc cgtaaccggg agacatacgg atcaaagcgt tcacctgacc
     4741 gacttcccca cgtactccaa tgagttgata gacaagaccc tggaggaaaa aatggaaatt
     4801 gcccagacag tctgctcgct ggttctgggc ctgcgtcgta aggttaacat taaagtccgg
     4861 cagccactga gcaaaatcat gatccctgtt cttgatgaaa ggttccgcgt gcagcttgat
     4921 tccgttaaag acctcattct tagcgaggtt aacgtaaagg agcttgaata cctgtccgat
     4981 tcgtcaggca tactggtgaa aaaggttaag cccaatttta aatcgctagg cccgcgtttt
     5041 ggtaaatcaa tgaaaagcat tgccactgcc ctgtcggagt tgtcacaagc cgatatcaac
     5101 acctttgagc gtagcggcaa gttaacctta aacattccag aaggaagcat tgatctgctg
     5161 ctcagcgatg tagaagtgct gtcggaggat atccctggct ggctggtagc cagcgaaggc
     5221 cgtataaccg tagctctcga tattaccata acagaggctt tacggcttga gggcatagcc
     5281 cgtgaactca tcaacagggt tcagaacata cgtaaggatt cggggtttga ggtaaccgac
     5341 aaaattcgca tcaccctgaa gaaacacgat aagttgaacc ctgccgtgga agcccattac
     5401 gattatattg ccggacaaac cctggccagg gagataattt taaccgaaag tgaactaaat
     5461 ggcaacgcca tcgttttaga ccttgacgat gacttgataa cctcgatttg tgtggaaaaa
     5521 atcaacgggt agcgatattt tttgaaacga tattttttat aattttagca aattttacag
     5581 ccatgggaga gaaaacaaga tattcggatg aggagctggc ggaatttaag gagttgattc
     5641 tgcaaaagct cgaaaaggcg cgcaacgact atgaaattct aaaaagcgcg ataaccatga
     5701 gcgaaagcaa cgatactgag gatacttcgc ccacttttaa agtgctcgag gagggagctt
     5761 caaccctttc gaaagaggaa gccggaaagt tggcccagcg ccagctgaag tttattgaac
     5821 atcttgaggc tgcgttggtt cgcatagaga ataaaaccta cggtatctgc agggagacag
     5881 gcaagctgat ctccaaggag cggctacgag ctgtgccaca tgccacccta agcattgacg
     5941 ccaaaaacaa ccagcggtag ctgattaatc gatacatcca tgaaatgcgg ccttttggcc
     6001 gcttattttt ttttagtagc tttgcgcaaa tttttacact aacctatgcg gcttacaaaa
     6061 cagaccacct cggcactggt agttattaca gttcttctta ttgatcagct acttaaaatt
     6121 tggattaaaa cccattttgc cattggcgac gagatgaggg tatttggaaa ttggttcatc
     6181 ctgcatttta ccgaaaatcc gggcatggca ttcggaatga atttttgggg agtatggggc
     6241 aagctgatct taagtatttt caggttggca gctattgttc ttattggttg gtacctgaac
     6301 catgtcatca aacagggagc caaaacaggc ttggtggttg gcatatcgtt ggtaatggct
     6361 ggtgccatag ggaacatgat cgacagcgct ttctacggcc tactgttcga ttcgggaacc
     6421 gtttacaacc acgatatgaa cggatgggtt tcctactacg gcgtatccaa gctaaatttt
     6481 gatggctatg caggtttttt aagggggtgt gtggttgata tgctttactt tccagtgatt
     6541 aatacggtta ttcccagctg ggtgccaatt tggggagggc accaatttca attcttccgt
     6601 cctgtattca atattgccga ttcggccatt accacagggg ttatttacct gatcctgttc
     6661 caaaggaaca acattattat aaaggacaaa ggaaaagggg taaaggggta aaggttaata
     6721 caaggttaaa ggttaaagga taaaggataa aaaaattcaa gcaggattca aaaacctgtc
     6781 cgcctactgg ctggattcca gatttaagaa cctgtccgcc tgctagcgga acttgtccgc
     6841 ccaccgtcta aacttgtctg gtggtttaag ccccgaaggg gcgtaataag tagcaagggg
     6901 cgcagcccct tgtatggagc gaacgatttt tacaagccct gaaagggcga aataggttta
     6961 aaaagaagag cctggagagc gtttaaacaa gagccccgaa ggggcggaat aagtagcaag
     7021 gggcgcagcc ccttgtatgg agcgaacgat tttttacaag ccctgaaagg gcggaatagt
     7081 tttgaaacga agagcctgga gagggtgtgt tggcgatgca agtagcggct ttcccaaaga
     7141 gctgttc
//
LOCUS       DAOPHX010000122         7110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_13530_length_7110_cov_7.993196, whole genome shotgun sequence.
ACCESSION   DAOPHX010000122 DAOPHX010000000
VERSION     DAOPHX010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7110
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13530_length_7110_cov_7.993196"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            482..2386
                     /locus_tag="PK502_08805"
     CDS             482..2386
                     /locus_tag="PK502_08805"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HPH00953.1"
                     /translation="MSVGYSEESIKTLEWQDHIRKRPGMYIGKLGNGSQPDDGIYVLL
                     KEVVDNSVDEFMMGAGKRIEISVVDHTVSVRDYGRGIPQGKLMDVASKMNTGAKYDSK
                     AFKKSVGLNGVGIKAVNALSKKFIIRSIREGIAKEIEFSAGLPVREQNYASNGELTGT
                     FIEFLPDDAVFGEYAFQDEYIEQMIRNYTYLNTNLTITFNGKEFVSKNGLLDLLNENL
                     GGEPLYPIIHLTGNDIEVAITHSSGYGEDYYTFVNGQHTIQGGTHLTAFRESYVKTIR
                     EFYKKDFDPADVRMSLIAAISIKVEEPIFESQTKTKLGSKDMSPDGPSIRQFIGDFIK
                     KELDNYLHKHSETASILLQKILESEKERKAISGIKKIARERAKKASLHNKKLRDCRVH
                     YNTSDPRAEESTLFITEGDSASGSITKSRDVNTQAVFSLKGKPLNTYGLTKKIVYENE
                     EFNLLQSALDIEDDMDNLRYNRIVIATDADVDGMHIRLLLISFFLQFFPELIRQEHLF
                     ILQTPLFRVRNKKKTSYCYSSEEREKAIRELGPNPEITRFKGLGEISPEEFANFIGPD
                     MRLDPVRLTKDDQIFALLEFYMGKNTPLRQDFIINNLRIEEDIVEEDTIHFEDGNVTA
                     EASFQHDTLV"
     gene            2409..5051
                     /locus_tag="PK502_08810"
     CDS             2409..5051
                     /locus_tag="PK502_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HPH00954.1"
                     /translation="MRLDDFDADELLVGGEKNTSPEEQAKIERLTADTVKRTQLTGMY
                     KNWFLDYASYVILERAVPHLDDGLKPVQRRILHAMKRLDDGRYNKVANIIGYTMQYHP
                     HGDASIGDALVQLGQKNLLVDVQGNWGNILTGDGAAAPRYIEARLSKFANDVVFNPKT
                     TEWMLSYDGRNQEPVTLPVKFPLLLAQGVEGIAVGLASKILPHNFNELIDAAVDYLRG
                     KEFEIYPDFPTGGMADCSRYNDGLRGGSVKLRARIQKVDRKTLVITELPFGKTTSSLI
                     ESIVKASEKGKIKIKRIDDNTAGNVEILIHLPPEANPDMTIDAMYAFTDCEISISTNA
                     CVIYEDKPCFMGVKDILKASVERTKELLTRELNIRLGELEEEWHFSSLEKVFFEKRIY
                     RELEKDTETWDMVIDAIERGFDPYRKLFRRPITRDDVLKLTEKPVRKISKFDIKKADE
                     HILAVEAEMAEVGKNLANVVDYTINHYQQIKKKYGKGRERKTELRSFDSIEATSVVAA
                     NEKLYVNRSEGFVGTGLKKDEYICDCSDIDDIIVFLRNGKYVVQKVQEKAFVGKDIIH
                     VAVYRKNDRQTTYNVLYRDGLNGDIMMKRCTISGTTRDKEYDITKGTAGSQILYFNAN
                     SNGETDILKVFLKPKPRLRNLILELDFSQLAVKGRDSQGNIFTRHAIHKIQVKEKLAG
                     QVEAQKVWYDPETLRLYKEAKGNFLGEFMPGEKILIANSDGTYFVAGTEYSLRFNAPP
                     LVVEKFNPNKIYSAVYFDGEQNYFYLKRFTFEPTEKIQPFIPDDGKSYLVELNSDLYP
                     NLLVRFGGKHASHQPETIDVDAFIAAKSYRAKGKRISTLNVKKVEFTEPLQKEASAFS
                     TIAGNNNAQLEEEDDGGAAEAVGK"
     gene            5377..6147
                     /locus_tag="PK502_08815"
     CDS             5377..6147
                     /locus_tag="PK502_08815"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00955.1"
                     /translation="MKTNLVKVALMAAAFVVGFSSCDDDDPTYTVTLAVNPSNAGTVT
                     GGGDYKKGDIIQLTATANENFYFVSWTEGTNELSTEATFDYTVGDKDVTLTANFNEKK
                     IVTLGAQSNTTFPGFLSVSEGATYNFNEASSHQAAVDIFCFYEVGNDIALASPGSNIT
                     GIFGGTETDPANWTTKNTTLFNQVTTLTVAQFDTIPNGDANIQTYYSADAARKKAKLL
                     IKDNIYAFKTQNGTYGLLKVIDVSVGADGFIKFEYKTK"
     gene            6218..>7110
                     /locus_tag="PK502_08820"
     CDS             6218..>7110
                     /locus_tag="PK502_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00956.1"
                     /translation="MRKLTICLIAIFLLAACSEKESVDTPPSITFQTGSYTQDGEAVP
                     LGGKLTFGITANGGSEPLTNIRIQRVAGGNVITEVDKGLFVKNEDYPYVVNAVKSDAS
                     EEVWRFMAMNSKRDSVEITRTVLLGEGVAYGPIRHFPSVKIGMQNNTEYPHFLCLSTG
                     TTYSNEEVAGNEASIDLLAFVYQTSGIWSPTLNCPQYTTAPSYYPIVGSWTTRNSTLY
                     DYNAVDNNLVSLDEFNAATNDSLLVASFKPQSSSGNCKFCFTGKIVPFKTQLGKYGLI
                     RIIHADQVAEGYMELEIKIQE"
BASE COUNT         2100 a         1729 c         1624 g         1657 t
ORIGIN      
        1 ctactctagt ctcttcattt caaacctatt ccgccccttc agggcttgta aaaatcgttc
       61 gctccataca aggggctgcg ccccttgcta cttattccgc cccttcgggg ctaaaatccg
      121 tcaggaaccg gtcaagtaca gacggtgggc ggacaagttc cgtcagcagg cggacaagtt
      181 cttgaatctt gaatctgaaa tctgaaatct ggaatccgtc aggagccgga caagttccgc
      241 cggtaggcgg acaggttctg gaatccgcca ggtaaaagac aagttccgtc gttagccagg
      301 caatttttta tcctttatcc tttaaccttt aaccttgtat taacctttat ccttgtcttg
      361 ccttttccca gcagagcgta aaagttcaaa aaaaagcctt acttgttgat atttattgcc
      421 agcagcagtg gctagcatat ttggaataat acctactttt gctgcaaatt caaacgtagc
      481 tatgagcgta ggttactcgg aggagagcat taaaacactt gaatggcagg atcatattcg
      541 caagcggccg gggatgtaca tcggcaagct gggcaacggc tctcagcccg atgatggtat
      601 ttacgttcta cttaaagagg tagttgacaa ctccgttgat gagttcatga tgggcgccgg
      661 taaacgaatt gaaatatccg tagttgatca taccgtttct gttcgagact acggaagggg
      721 catcccgcag ggaaaactca tggatgtggc ctctaaaatg aataccggag ctaagtacga
      781 ctctaaggca tttaaaaaat cggttgggct taacggtgta ggaataaagg ctgtaaatgc
      841 cctatcgaaa aagttcatca tacgtagcat tcgcgagggc attgccaagg aaatagagtt
      901 cagcgcaggg ctccccgtta gagagcaaaa ctacgcctcc aatggagagc ttaccggaac
      961 attcatcgag tttttacccg atgatgccgt tttcggcgaa tacgcctttc aggatgagta
     1021 cattgagcag atgatccgta actacaccta cctgaacacc aaccttacca tcaccttcaa
     1081 cggcaaggag tttgtatcga aaaacgggct gctcgacctg cttaacgaaa accttggcgg
     1141 cgaaccgctc taccccatta tccaccttac cggtaacgat attgaggtgg ccattacgca
     1201 cagtagtggt tatggtgaag actactacac ctttgttaac ggacagcaca ccattcaggg
     1261 aggcacccac ctaaccgcat tcagggaatc ctacgttaaa accattcggg aattctacaa
     1321 aaaggacttc gatccggccg atgttcgaat gtcgctcatt gcggccatca gcataaaggt
     1381 ggaagagcct atttttgaat cgcaaaccaa aacaaagctg ggaagcaagg atatgagccc
     1441 cgacgggcca tccattaggc agttcattgg tgattttatt aaaaaggagc tggacaacta
     1501 ccttcacaag cacagcgaaa ccgccagcat cctgctccaa aagattcttg agtcggaaaa
     1561 ggagcgtaag gctatctccg gaataaaaaa aattgcccgt gaacgggcaa aaaaggccag
     1621 cctgcataac aagaagctgc gcgattgccg tgttcactac aacaccagcg atccccgtgc
     1681 cgaggagtca accctgttta tcaccgaggg ggattcggcc agcggttcca ttaccaagtc
     1741 gcgcgatgtg aacacccaag ccgttttctc cctgaagggg aaacccctca acacctacgg
     1801 cctgaccaag aaaatagttt acgagaacga agaattcaac ctgctgcaat cggcgctgga
     1861 cattgaggac gacatggaca acctccgcta caaccgcatt gtgatagcca ccgatgccga
     1921 tgttgacggg atgcacatcc ggctactgct catcagcttt ttcctgcaat tcttccccga
     1981 gctaatccgg caggagcacc ttttcatcct tcaaacgccg ctgttcaggg taaggaataa
     2041 gaaaaaaaca tcatactgct actcttccga agagcgcgag aaggccatta gggaacttgg
     2101 ccctaacccc gaaattaccc gatttaaagg ccttggtgaa atatcacccg aagaatttgc
     2161 caacttcatt ggccccgaca tgcggctcga ccccgttcgg cttaccaagg acgaccaaat
     2221 atttgccctg ctcgaattct acatgggtaa aaatacccct ttacgtcagg atttcatcat
     2281 caataacctc agaatagaag aggatatagt ggaggaggat actattcatt ttgaagatgg
     2341 gaatgttacg gctgaggcaa gctttcagca cgataccctg gtataactaa caaacgctgc
     2401 agcacgccat gcgacttgac gattttgatg ccgacgagct actcgttggc ggcgaaaaaa
     2461 atacttcacc cgaagagcaa gccaaaattg aaaggctaac cgccgatacg gtaaaaagaa
     2521 cccagctaac cggaatgtac aaaaactggt ttcttgacta cgcctcgtac gttattctgg
     2581 aacgggctgt acctcacctt gacgacgggc tgaagcctgt tcagcgtcgc attctacatg
     2641 ccatgaaacg ccttgatgat ggccgctaca acaaggtggc caatataatt ggctacacca
     2701 tgcagtacca tccccatggc gacgcctcca ttggtgatgc gcttgttcaa ctggggcaaa
     2761 aaaacctgct ggttgacgtt cagggtaact ggggcaacat ccttacgggt gatggagcgg
     2821 ctgcaccccg gtacatagag gcacgcctgt cgaaatttgc caacgacgtg gtgtttaacc
     2881 ctaaaaccac cgagtggatg ctcagctacg acggtagaaa ccaggagccc gttactctcc
     2941 ccgtaaagtt tccactgttg ctggcacaag gcgttgaggg cattgccgtc ggattggcct
     3001 caaaaattct gccccacaac ttcaacgagc tgattgatgc agccgttgac tacctcagag
     3061 gtaaagaatt tgagatctac cccgatttcc caacaggcgg catggccgat tgcagccgtt
     3121 acaacgatgg cctcaggggc ggctcggtta agttgcgtgc ccgtattcaa aaagttgacc
     3181 ggaaaacatt ggtcatcacc gagttaccct ttggcaaaac tacctcatcg ctcatcgaat
     3241 ctattgttaa ggccagcgaa aagggaaaaa tcaaaataaa gagaattgac gacaacacgg
     3301 ccggaaatgt tgaaatactc attcaccttc cgccggaggc caatcccgat atgaccatcg
     3361 acgccatgta tgcctttacc gactgtgaaa tatccatttc taccaacgca tgcgttattt
     3421 acgaggacaa accatgcttc atgggggtta aggacattct aaaagcttcg gttgagcgga
     3481 ccaaggagct gcttacccgc gagctgaaca tccggttggg cgaacttgaa gaggagtggc
     3541 atttctcctc gcttgagaag gtattctttg aaaagcggat ataccgtgag ctggagaagg
     3601 ataccgaaac atgggatatg gtgattgacg ccattgagcg aggttttgac ccctacagga
     3661 aacttttccg ccgaccaata acccgcgacg atgtgctgaa gcttacggaa aaacctgtca
     3721 ggaaaatttc aaaattcgac ataaagaaag ccgacgagca cattcttgcc gtggaggctg
     3781 aaatggcaga ggttggaaaa aacctggcca acgttgtgga ttacaccatt aaccactacc
     3841 agcaaataaa gaagaagtac ggtaaaggca gggaacgcaa aaccgagctt cgcagcttcg
     3901 acagcattga ggccaccagc gttgttgctg ccaacgagaa actatacgtt aaccgttccg
     3961 agggctttgt gggaaccggc cttaaaaaag acgagtacat ctgcgattgc tccgacattg
     4021 atgacattat cgttttcctg cgcaacggaa agtatgttgt tcagaaggtg caggaaaagg
     4081 catttgttgg caaggacatc atccatgtgg cagtttacag gaaaaatgac aggcaaacta
     4141 cctacaacgt gctgtaccgt gatggtttaa atggtgatat catgatgaag cgctgcacca
     4201 taagcggcac cacccgcgat aaggagtacg acatcaccaa gggaacagct ggctcacaga
     4261 ttctctactt taacgccaac tccaacggcg aaacagatat tttaaaggtt ttcctcaaac
     4321 ccaaacctcg ccttcgaaat ctgattcttg agctggattt tagccagctg gctgtgaagg
     4381 gaagggactc gcagggcaac atttttaccc gccatgccat tcataaaatt caggtgaagg
     4441 aaaaattagc cggccaggta gaagcccaaa aagtatggta cgaccccgaa acactccggc
     4501 tctacaagga ggcaaaaggt aattttttag gtgagtttat gcccggcgaa aaaattttga
     4561 tagcaaatag cgatggaacc tatttcgtgg ccggaacgga gtactccctg cgatttaacg
     4621 ctcctcccct tgtggtggaa aaattcaacc ccaacaagat ttactcggct gtttacttcg
     4681 atggtgagca aaactacttt taccttaagc ggttcacctt tgaacccacc gagaaaatcc
     4741 aaccctttat tcccgacgat ggcaagtcgt accttgtgga gctgaacagc gatttatacc
     4801 ccaaccttct ggtacgattc ggtggaaagc atgccagtca ccaacccgaa accattgatg
     4861 tggatgcatt tatagcagct aaaagttacc gcgctaaggg gaaacgaatt tccaccctca
     4921 acgttaaaaa agtagagttt accgaacccc tgcaaaagga agcttcagcc tttagcacta
     4981 tagccggcaa taacaatgca caactcgaag aagaagatga cggaggagct gctgaagcag
     5041 ttggtaagta gcaaagtagc gctacatgct agctcacatg gcaaagttct taccctggca
     5101 tctaattagc cctgcagcca gctacaccaa gctgcaactt ttcaatttat tttgaaattt
     5161 ttaataatga tgtacctttg ccaatcgata tgtaataatt ttcataacaa aaagacacct
     5221 agcctcttaa caaggaagga gaacataact catcaacgtt gaaatgaaga ggggggccgt
     5281 gatagctgta gaaatacgat tttaaactcg aggaaaagtg catttgatac gcatattaaa
     5341 ccaaatgtta aactactcaa aaacagcatt aaaagtatga aaacaaatct tgttaaggtt
     5401 gcccttatgg cagctgcatt tgtcgttggt ttttcgagtt gtgacgacga cgatccaacc
     5461 tacaccgtaa cccttgccgt aaatccatcc aacgcaggaa ccgttaccgg tggaggggat
     5521 tacaaaaagg gcgacattat ccagcttact gccacagcca acgagaattt ctactttgta
     5581 agctggacag aaggaaccaa cgagctaagc accgaagcca ccttcgatta cactgttggg
     5641 gataaggatg ttacgctaac cgccaatttc aacgagaaaa aaattgttac actaggtgca
     5701 caatccaaca caacttttcc aggatttctt tccgtttccg aaggtgccac ctataacttt
     5761 aacgaagctt ccagccacca ggcagctgtt gatatttttt gcttctatga ggtggggaat
     5821 gatatagcgc tggccagccc tggctcaaac atcaccggaa tctttggggg aaccgaaacc
     5881 gaccctgcca actggacaac caaaaacacc acccttttta accaggtaac cactcttact
     5941 gtcgcccagt ttgacaccat ccccaacggc gatgcaaata ttcaaaccta ctatagtgcc
     6001 gatgcagctc gcaaaaaggc aaaattgctt ataaaggaca acatctacgc ctttaaaact
     6061 cagaatggaa cctacggact gctaaaggtt atagatgtaa gcgtaggagc cgatggtttt
     6121 ataaaatttg aatacaaaac aaaataacca gttaatttta aactcattaa ccctgaaagg
     6181 aagcaaactt ttcggggtta ctattttttt taaaactatg cgaaagctaa ctatttgcct
     6241 aatagcaatt ttcctgctag cagcttgcag tgaaaaagag tcggtggata cccctccatc
     6301 aattactttt caaaccggga gctacacgca ggatggtgaa gcagttcctc taggcggtaa
     6361 gctaacgttc ggaatcactg caaatggagg aagtgagcca cttacaaata tcagaattca
     6421 acgggtagcc ggaggcaacg ttattactga ggtagataag gggttattcg taaaaaacga
     6481 agactaccct tacgtggtta atgcagttaa atctgatgct tcggaggagg tgtggcgatt
     6541 tatggccatg aattccaagc gcgacagtgt tgaaattacc cgaaccgtgc ttttaggtga
     6601 aggtgtagca tacggaccaa taaggcattt cccctccgtg aaaattggaa tgcagaacaa
     6661 cacggagtac ccacactttc tctgcctaag cactggcacc acctacagca atgaagaggt
     6721 tgccggcaac gaggcttcca ttgatttgct ggcatttgtt taccaaacca gcggcatctg
     6781 gtcgccaacc cttaactgcc cgcagtacac aaccgcacca agctactacc ccattgtggg
     6841 cagctggaca acacgaaact caacactcta cgactacaac gcagtggata acaacctggt
     6901 tagccttgat gaatttaatg ccgctaccaa cgacagcctg ctggtggctt catttaaacc
     6961 tcaaagttca agcggaaatt gcaagttctg cttcaccggt aaaattgtcc ctttcaaaac
     7021 ccagctgggc aagtacggtc ttattaggat tatacatgcc gatcaggttg cagaaggata
     7081 catggagctg gaaattaaaa ttcaggagta
//
LOCUS       DAOPHX010000123         6838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_14265_length_6838_cov_4.923780, whole genome shotgun sequence.
ACCESSION   DAOPHX010000123 DAOPHX010000000
VERSION     DAOPHX010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14265_length_6838_cov_4.923780"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            448..1584
                     /locus_tag="PK502_08825"
     CDS             448..1584
                     /locus_tag="PK502_08825"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPH00957.1"
                     /translation="MRIAQIRLLAASMLITTSVLHTGCSTNTRSSEEITIGVEELCSK
                     DGKSLIITHPTVSTIKTFDKLVKNGLLVLPNRTRIVGIYHSGENYDYALSQQYIAENA
                     IDYFYLLPIKPEITPDSIFHRNACSGIFEKLVSTCAGIIFTGGPDIPPSIYNEPTSLL
                     TQITDPERHSMEISFLFHLLGGRRDTLFKPLLADSSNLAVLGICLGMQSINVAAGGTL
                     YQDIPTDIYHLSTVESVLAQEINQQHRNYNCSYGYEEQLLGGTFHQVEFSDELFSSIA
                     AGKKPFVLSSHHQCLKMLGDNIRVAGYSADGKVVEAIRHIKYKNVVGVQFHPEASLLY
                     DRNELFLITPNQQSGQSYLNLYPGESGENFHREFWRWFSALLVK"
     gene            complement(1645..2478)
                     /gene="pyrF"
                     /locus_tag="PK502_08830"
     CDS             complement(1645..2478)
                     /gene="pyrF"
                     /locus_tag="PK502_08830"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPH00958.1"
                     /translation="MTPEQLFDQIRLKRSFLCVGLDTELTKIPEFLLTKDDPIFEFNK
                     AIVDATHDLAIAYKLNLAFYEVHGATGWNSLVKTIKYIRRFYPSMFIIADAKRGDIAS
                     TARMYANAYFKSLDCDAVTVSPYLGHDSVTPYLNFEGKWTILLAITSNDGFRDFQLAV
                     NRDTGNFMFEDVVAKSLQWGNEANIMYVVGATHPEYFAAVRKIVPNHFLLVPGVGEQG
                     GSLEKVAEHGLNNRCGLIVNSSRGIIYADITSRFTSAARARALKLQQQMEFILTAAKL
                     V"
     gene            complement(2491..3579)
                     /gene="prfA"
                     /locus_tag="PK502_08835"
     CDS             complement(2491..3579)
                     /gene="prfA"
                     /locus_tag="PK502_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPH00959.1"
                     /translation="MSDNTITRKLEGVRQKFEEIGQKITDPAVMADMKKFVSLNKEYK
                     ALEPLVAAYEKYKNILSNIESSRQILSSEKDEELREMAKEELDALTQEQEKLEEEIKL
                     LLLPADPEDSKNAVMEIRAGTGGDEASIFAGDLFRMYTKFFERKGWKYEVTSSSEGTV
                     GGYKEIVMSVTGNGVYGILKYESGVHRVQRVPQTETQGRIHTSAATVAVLPEAEEFDI
                     DLKMEDIRKDIFCSSGPGGQSVNTTYSAIRLTHIPSGIVVQCQDQKSQLKNFDKALAE
                     LRTRLYNLEYQKYLDNLSSKRKTMVSTGDRSAKIRTYNYPQGRVTDHRINLTLYNLSA
                     IMDGDIQELIDKLQLAENAERLKAESTD"
     gene            complement(3829..4998)
                     /locus_tag="PK502_08840"
     CDS             complement(3829..4998)
                     /locus_tag="PK502_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HPH00960.1"
                     /translation="MNDQHRYNQRGVSASKEDVHNAIKNLDKGLYPKAFCKVVPDILA
                     GNPDYCLVMHADGAGTKSSLAYAYWRETNDMSVWKGIAQDAVVMNLDDLLCVGVTDNI
                     LLSSTIGRNKNLIPGEVISAIVEGTAQFCEEMQKYGIHITLTGGETADVGDLVRTVIV
                     DSTVIARLKRAEVISNSNIQDGDVIIGLSSCGKANYEQEYNGGMGSNGLTSARHDVFS
                     SYLADKYPETFDPSVPRELVYSGSFKLTQMVDEVGITAGKLMLSPTRTYAPVVKEVLD
                     SYRPVIHGLVHCSGGAQAKVLNFVDNLHVVKDNLFPIPPLFRIIQSESNTSWREMYKV
                     FNMGHRFEIYVFPEFADDIIAISKDFGVDARVVGYCSSFQGKKLTIKSEKGEFYY"
     gene            complement(5076..5432)
                     /locus_tag="PK502_08845"
     CDS             complement(5076..5432)
                     /locus_tag="PK502_08845"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HPH00961.1"
                     /translation="MFRFVKILLTLVAVLLAEITFAQKFFEVEKVEDAQVKFYAVDDP
                     KDADLLFCIIYEEKLITKAGIMMEVETPREAQVLIIFVDDPALADIKVWLVEKPEEVV
                     WKNESKRKLLSVGGLK"
     gene            complement(5655..6284)
                     /locus_tag="PK502_08850"
     CDS             complement(5655..6284)
                     /locus_tag="PK502_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPH00962.1"
                     /translation="MKQLITPILVCLSGILLAQPAEVLTLNKPSLSRGKNIMEALASR
                     ASATEFTSDELSLQDISDLLWAANGINRPEIGKKTAPSATNSQDIDVYVVTSKGAYIY
                     EPVQHQLKLVNAGDFRELVAGRQKNFANAPLFCIMVSDISRFRGNDDSQKMRWAAMDA
                     AIVSQNINIFCSGVGLRTRTRAFMEADKLREALKLTPSQFPMLNNPVSN"
     gene            complement(6354..>6838)
                     /gene="mltG"
                     /locus_tag="PK502_08855"
     CDS             complement(6354..>6838)
                     /gene="mltG"
                     /locus_tag="PK502_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPH00963.1"
                     /translation="KEYESFWTPEREQKAYRLGITRIDVSILASIVEEETRMVTEMPV
                     IAGVYINRLHRGIPLQADPTVRFALGDFSIRRILSKHLQLDSPYNTYKYKGLPPGPIR
                     CPSIRAIDAVLNAEKHSYLYFCAKPDFSGYHVFARTLMEHNRNAEAYRRALNREKIFR
                     "
BASE COUNT         1901 a         1585 c         1460 g         1892 t
ORIGIN      
        1 agttttgacc atggaatata taattcaatt catgataact acttggtgcg ggaatgggtt
       61 gattttaagg gaagaaatga taaaaaaaga atatctaaag acagtattga tattaacgaa
      121 ttaaaagaag caggattttt attaaataga tggaaataat tacgccgccc aacaggtggt
      181 atagcctact ggggctgatg atgcatatac gaaaattttt agtatattag tatgttttta
      241 acggcggata acgccaatgg cggttaactc cccagcgtaa gcaaccgaca gccgctagca
      301 tttacaccat aaatcaacat gagagtattt tgtttactat tttcgggttt acttactgtt
      361 gcacgattag ctgcacaagc tgccagtaac cggacaatgt ttgatgagtt gaatagattt
      421 aattctgcct attaaaatcg gaaaactatg cgcatagctc aaatccggct gcttgcagca
      481 tcaatgctca tcacaaccag tgtgctacac accggttgct ctaccaatac acgcagttca
      541 gaagaaatta ccattggagt tgaagagctt tgctcaaagg atggtaaatc gcttattatc
      601 acccacccaa ccgttagcac catcaaaaca ttcgacaagc tggtgaaaaa cggcctcctt
      661 gtactgccaa acagaacaag aattgttgga atttaccaca gtggagaaaa ttacgattat
      721 gccctctcgc agcagtacat tgctgagaac gccattgact acttttatct tctgcccata
      781 aaacccgaaa tcacaccgga cagcatattt caccgtaacg cctgtagcgg aatatttgag
      841 aagctagtaa gcacatgtgc tggtataatt tttaccggag gtcccgatat accgccttca
      901 atatataatg agcccaccag cctgctaacc caaataaccg acccggaaag gcatagcatg
      961 gaaatttcct tcctcttcca cttactgggt ggaaggcgtg ataccctttt taagccgctg
     1021 ctggctgata gctcaaacct tgctgtgctg ggaatttgcc tgggaatgca gagcattaac
     1081 gtggcagccg gaggaacgct ttaccaggat attccaaccg atatctacca cctttcaact
     1141 gttgaatcgg tgctggcaca ggaaatcaac cagcagcacc gtaactacaa ctgcagctat
     1201 ggttacgaag aacagcttct cggtggaact tttcatcagg tagagttttc ggatgagctg
     1261 ttcagctcaa tagctgccgg taaaaagccc tttgtgctta gtagccacca ccagtgtttg
     1321 aaaatgcttg gcgacaacat tagggtagca ggatattcag ctgacggtaa agtggtggag
     1381 gcaatacgcc atatcaagta taaaaacgtg gttggtgttc agtttcaccc cgaggcatca
     1441 cttctgtacg acaggaatga gctgtttttg attaccccaa accaacagtc aggccaatcg
     1501 tacttgaacc tttatcctgg ggaatccggt gaaaattttc acagggagtt ctggagatgg
     1561 ttttcggccc tgcttgtaaa gtaaaaaatc cgtattgtgg ctgtagagca ttgaatatct
     1621 accaacgtgt aaaatgaaaa agggctaaac aagttttgcc gcagttaaaa taaattccat
     1681 ctgctgctgc agctttaggg cacgcgctct agctgcgctc gtaaatcggc tggtaatatc
     1741 agcataaata atgccccttg aagaattaac aattaacccg cagcggttat ttaaaccatg
     1801 ttcggcaacc ttttcgaggc taccaccctg ttccccaaca cccgggacaa gcagaaaatg
     1861 gttaggaaca atttttcgta cagcggcgaa atactcggga tgagttgccc caaccacgta
     1921 cattatatta gcctcgttgc cccactgcaa cgacttggca acaacatcct caaacataaa
     1981 gttacctgta tccctgttaa cggccagctg aaaatcgcga aagccatcgt tggaggtgat
     2041 ggccaacagg atagtccact tgccctcaaa attcaggtat ggcgtaacgg aatcgtgacc
     2101 caggtatggc gaaacggtaa ccgcatcaca atccagactt ttaaagtagg catttgcata
     2161 catgcgtgca gtgctggcaa tatcgccccg tttggcatcg gcaatgatga acattgaggg
     2221 gtagaagcgt cgaatgtact tgatggtttt aaccaggcta ttccatccgg tagcgccatg
     2281 cacctcgtag aacgccagat tcagcttata ggcaatggcc aaatcgtgag tggcatcaac
     2341 aattgccttg ttaaactcaa aaatcgggtc atccttggta aggaggaact cgggaatttt
     2401 tgtaagctcc gtgtccaacc ccacacatag gaaactccgt ttcaatctta tctggtcgaa
     2461 tagctgttcg ggagtcattc tggctactgg ttagtctgtt gattcggctt ttagcctttc
     2521 ggcattctca gctagctgaa gcttgtcaat tagctcctga atatctccat ccatgatggc
     2581 agagaggttg taaagggtaa ggtttatcct atgatcggta actcgtccct gcgggtagtt
     2641 gtaggttcgg attttagccg atcggtcgcc ggttgaaacc atggttttac gcttggacga
     2701 aaggttgtcg aggtactttt ggtactcaag gttatacaag cgggtacgta gctcggccaa
     2761 cgccttgtca aagtttttaa gctgtgattt ttggtcctgg cattgaacaa caattccact
     2821 gggaatatgg gttaaacgaa tggcggaata ggttgtatta acgctttgac cacccggacc
     2881 cgaagagcaa aaaatatcct ttcggatatc ctccatttta aggtcaatat cgaactcttc
     2941 agcctcggga agcacagcaa cagtggctgc tgatgtgtga attcttccct gagtttcggt
     3001 ttgtgggact ctttgcaccc gatgtacacc cgattcgtac ttcaaaatgc catatacgcc
     3061 gtttccggta acactcatca caatctcctt gtaaccacca accgtaccct ccgaagagct
     3121 ggtaacctcg tacttccaac ccttccgttc aaaaaacttg gtgtacatgc ggaaaaggtc
     3181 gccggcaaaa atactggcct cgtcgccacc tgttccggcg cgtatctcca tcacggcatt
     3241 cttggagtct tcagggtcag ccgggagcag taataatttt atctcttcct ccagcttttc
     3301 ctgctcctgg gtaagggcat cgagttcctc cttggccatt tctcgcagct cctcatcctt
     3361 ttcggagcta agtatttgcc gggacgattc gatgttgctc agtatattct tgtacttttc
     3421 gtaggcagca actaagggtt ctagcgcttt gtactcctta ttcaggctta caaatttttt
     3481 catatcggcc attacggccg gatcggttat tttctgccca atttcttcga acttttgcct
     3541 tactccttct agcttcctgg taattgtatt gtcggacata gactggtaat ataatgcgca
     3601 aaggtaagca taaatttaga aagcgagtat tcttccacca ctcataatgc tttacccaac
     3661 aaggtgcaag gttgatatta ctgtgacgtt cagatctttg gtgcagggta taatacacct
     3721 ttttaataat gtggtaaaca gctgattgaa tgactaataa cagcaagcac ggagagggct
     3781 aaacaaaaat tttattcttc acgttcaaaa gagctcaaac accactttct agtagtaaaa
     3841 ttcacctttt tctgatttaa ttgttagctt tttcccctga aagctgctac agtagccaac
     3901 aacccttgca tctacgccaa aatccttaga aatggcaata atatcatcgg caaattcagg
     3961 gaaaacgtat atttcaaatc ggtgccccat gttgaaaact ttgtacattt ccctccacga
     4021 tgtattcgac tcgctttgga taatcctaaa caaaggagga atgggaaaaa ggttatcctt
     4081 aaccacgtgt aggttatcaa cgaagttgag cactttagct tgagctcctc ccgagcaatg
     4141 cacaaggcca tgaatcacag gacggtagct gtcaagaacc tctttcacaa caggggcata
     4201 ggttcttgta ggtgaaagca tcagctttcc ggcagtaatt ccgacttcat caaccatctg
     4261 ggtgagctta aagctaccac tgtacaccaa ctcgcgagga actgacggat cgaatgtttc
     4321 gggatactta tcggccaggt agctgctaaa aacatcatgg cgggcagagg tgagcccgtt
     4381 gctacccatg cctccgttgt actcctgttc ataattagcc ttgccacacg atgataggcc
     4441 gatgataaca tctccatcct gaatgttact gttagatatt acctccgctc gcttcagcct
     4501 ggcaataacg gtagagtcaa caattacggt tctgaccaag tcgcctacgt cggctgtttc
     4561 tccacccgta agcgttatgt ggataccata tttttgcatc tcctcgcaaa attgagcggt
     4621 tccctcaact atcgcagaaa tgacttcgcc gggaataagg ttcttattcc ggccaatagt
     4681 agaggagaga agtatgttat ccgtaacgcc cacacaaagc aggtcgtcta aattcatcac
     4741 aacagcatcc tgagctattc ctttccaaac cgacatatca ttagtctcgc gccagtatgc
     4801 ataggccaac gacgactttg ttcctgcccc atcggcatgc atcaccaggc agtaatccgg
     4861 attgccagct aaaatatcgg gaacaacctt acaaaaagcc ttagggtata ggcccttatc
     4921 cagatttttt attgcgttat gaacatcctc ctttgatgct gaaactcccc gttggttgta
     4981 tcggtgttga tcgttcatat aggaaattga tagtttatgg ttcgagatta cctgcaccgc
     5041 tattacttca aattaaaaaa aagtagcccc catcattact tgagcccacc aacagaaagc
     5101 aactttcgtt tcgattcgtt tttccacacc acctcctccg gtttttcaac cagccaaacc
     5161 ttaatgtcgg caagagccgg atcgtcaaca aaaataatga gcacttgcgc ctccctgggc
     5221 gtttccacct ccatcataat gccggcctta gtaatcaatt tttcctcata gataatgcag
     5281 aacagcagat cggcatcctt ggggtcatca accgcataaa atttaacctg ggcatcctca
     5341 actttttcga cctcaaagaa tttctgggca aaagtaatct cagccagtaa aacagcaacc
     5401 agggttagaa gtatctttac gaaacgaaac atagaactgc ttgcaaattt aaccatgtat
     5461 agtgtaaact tttaccccgc tcaaaattaa aaaagttgca gcaagtgctc aaaacataac
     5521 ttgttcttgt tgctgatttg atgataagtt tttatctgct gaaagtttcc cgcacatcag
     5581 ggttcagtaa tgtcagtatt ataaaattcc agatggtaaa caagcaaaat tgcgttagga
     5641 actctacaat atgcctaatt gctcaccgga ttatttagca ttgggaactg tgagggggta
     5701 agttttagcg cctcacgtag cttatctgct tccataaaag ccctggttct tgttctcaag
     5761 cccaccccag agcaaaatat gttaatgttt tgcgaaacaa tggcggcatc cattgcagcc
     5821 cacctcattt tctgagaatc atcattaccc cggaatcgtg agatatccga aaccataata
     5881 cagaatagcg gagcgttggc aaagttcttc tgacggccag ccactaactc cctaaaatct
     5941 ccagcattaa ccagctttag ctgatgctgt acaggttcat agatataggc tccttttgaa
     6001 gtaaccacat aaacgtctat gtcctgagag tttgttgcag aaggtgccgt ttttttccca
     6061 atttcgggtc ggttaatgcc gtttgcagcc caaagcaaat cggaaatatc ctgcagggat
     6121 aactcatcgg atgtaaattc ggtggcggaa gctctgctgg ctagggcctc cataatgttt
     6181 ttccctctgg ataatgaggg tttatttaag gttaaaacct cggcaggttg ggccaggagg
     6241 attccgctaa ggcatacaag aattggtgta attagctgtt tcattgctgt aggttttaga
     6301 attagaaaca tggatgcaag ttagcaaacg catggatatt tctggcagca aagctacctg
     6361 aaaatttttt ccctattcaa tgccctgcgg tatgcctctg cattccggtt atgctccatg
     6421 agagttcggg caaaaacatg gtagcccgaa aaatcaggct ttgcacaaaa gtagagatag
     6481 ctatgtttct cggcatttag cacagcgtca atagctctaa tggagggaca tctaattgga
     6541 cctggtggaa gtcccttgta cttgtacgtg ttatagggcg aatcgagttg aaggtgtttg
     6601 gaaagaattc ttctgatgga aaaatcgcca agggcaaacc taacggttgg atcggcttga
     6661 agggggattc ccctatgcag cctattaatg taaactcctg caataacagg catttcagtt
     6721 accattctgg tttcctcctc aacaattgag gccaatatag aaacatctat ccttgttatc
     6781 ccaagccgat aggctttctg ctcgcgttca ggtgtccaga aactttcata ttcctttt
//
LOCUS       DAOPHX010000124         6778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_14453_length_6778_cov_5.547970, whole genome shotgun sequence.
ACCESSION   DAOPHX010000124 DAOPHX010000000
VERSION     DAOPHX010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14453_length_6778_cov_5.547970"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..1924)
                     /gene="ftcD"
                     /locus_tag="PK502_08860"
     CDS             complement(224..1924)
                     /gene="ftcD"
                     /locus_tag="PK502_08860"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPH00964.1"
                     /translation="MAEKKLIECVPNFSEGRDSGLIKQITDVIEAVEGVKLIDVDPGK
                     ATNRTVVTFVGTPDEVVEAAFRAVAKAKELIDMSRHHGAHPRFGATDVCPLVPVANIT
                     MDEVVEYARALAKRIGDELGIPVYCYEYAALEEKRRNLASCRAGEYEGLPKKLQDPEW
                     KPDFGPAQFLPKTGAIAVGARNFLVAYNVNLNTTSTRRANAVAFDIREKGRPMRVGNP
                     VTGKIATDENGKEIWIPGTLKACKAIGWFIEEYGIAQVSINLTDITVTPVHVAFEEAS
                     KKAQERGLRVTGSEIVGVVPLSAMLEAGKYFLRKQQRSVGIPDREIIRIAVKSMGLDE
                     LYPFDPDKKIIEYILEEKGTRQLAGMTLESFSRETASESPAPGGGSISAAMGAFGAAL
                     AAMVANLSAHKPGWDDRWEEFSTWAEKGKYYMEQLLALVDEDTRAFNRIMEAYGLPKG
                     TDLEKAARSKAIQEATIQAIEVPFRVMNLCYESLEVIRAMARQGNPSSVSDAGVGALA
                     ARSAVLGAFLNVKINSSGLKDKALVKDYLDRGNQIASKTIAMEEEILSDIEKNFFASL
                     "
     gene            complement(2080..3336)
                     /gene="hutI"
                     /locus_tag="PK502_08865"
     CDS             complement(2080..3336)
                     /gene="hutI"
                     /locus_tag="PK502_08865"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPH00965.1"
                     /translation="MQILLLNIKKLVQVEEKPVQWVAGQNMANLKCIDNAFLLMENGL
                     IKDFGSMRDVPSFAGLPSDVLIYNVNGRMVFPSFCDSHTHLVYAGSREVEYIDKIKGL
                     SYEEIARRGGGILNSAKLLSQTSEDELYEQALPRVKEIIRKGTGAVEIKSGYGLSTEN
                     ELKMLRVIRRLSEATPLTIKATFLGAHAVPAEYKGRQAEYVDQIINEMIPQVAAEDLA
                     DYIDVFCDRGFFTVEDTERILMAGMKYGLRPKIHANELDYSGGIQVGVKYNALSVDHL
                     EFTGDDEIQTLLGSETMPTLLPGAAFFLGMDNPPVRKMIDAGLPIALASDFNPGSSPS
                     GDMKFIMSLGCIRLKMLPEEVINATTINGAYAMGLSETHGSICRGKVANIFITKPIPT
                     LEYMPYAYTSDLVETVFLNGNITNVC"
     gene            3566..4489
                     /locus_tag="PK502_08870"
     CDS             3566..4489
                     /locus_tag="PK502_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPH00966.1"
                     /translation="MKRDIITIDREKCNGCGQCVGGCHEGALQLIDGKAVLVSELMCD
                     GLGACIGECPEGAITVERREADAYDEVTTLSQMVASGKNTTVAHLKHLKEYHQTDYLR
                     QAVEWLLSNRKNVSFSVDEVMQEVHNHSGSSCGCGNGGKHEPTQQSHHGKHVDGCPGS
                     AERSFGNNIRVEASKMVSGKSELTHWPVQLHLINPHSEHFKGSNLLLAADCVAYSQGS
                     FHSQHLAGKTLAIACPKLDSNKEEYIEKITAFIDDAQVDTITVMKMEVPCCGGLLQLA
                     RLARDKARRSVPIKVITVGIQGDILDSVWEN"
     gene            4629..6275
                     /gene="hcp"
                     /locus_tag="PK502_08875"
                     /gene_synonym="priS"
     CDS             4629..6275
                     /gene="hcp"
                     /locus_tag="PK502_08875"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPH00967.1"
                     /translation="MSMFCFQCQETAKGTGCTIKGVCGKSDDLANMMDLLMFVTKGIC
                     VYSTALREQKGYENPIVNKFVFDSLFTTITNANFDKQVISNRVSSALEIRETLRNEAS
                     LQGVRVDTSFEGCTWSGTPAEYEAKAALVGVLSEKNEDIRSLKELITYGVKGMAAYTE
                     HAYNLGHEDVQNYAFMQRALASITRNLSVDELVALTLETGKFGVNAMALLDKANTSSF
                     GNPEITKVNLGVRSNPGILISGHDLKDMEELLAQTEGTGVDVYTHSEMLPANYYPAFK
                     KYRHFVGNYGSSWWHQREDFETFNGPILFTTNCIVPPLNNATYKDKIFTTGAAGYPGS
                     KHVADRKPGQQKDFSEIIALAKTCKAPTQIETGEIIGGFAHAQVFALADKVVDAVKSG
                     AIRKFVVMAGCDGRMKDRTYYTEFAQKLPKDTVILTAGCAKYRYNKLALGDINGIPRV
                     LDAGQCNDSYSLAIIALKLKEIFQLNDINELPIAYNIAWYEQKAVIVLLALLYLGVKN
                     IHLGPSLPGFLSPNVTNVLVDNFGISGITTVDEDIKLLVG"
     gene            6373..6699
                     /locus_tag="PK502_08880"
     CDS             6373..6699
                     /locus_tag="PK502_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPH00968.1"
                     /translation="MNNEFEKEKKFNFLDEVDYSTGGVVSKNVIKKPTGNISLFAFEK
                     GEGLSEHTAPFDALLQVIDGEAKVTINGKPHDLKAGESIIMPANSPHAVFAAERFKMV
                     LTMIKE"
BASE COUNT         1825 a         1671 c         1595 g         1687 t
ORIGIN      
        1 tacaagcagc aaacctgcaa gaaaacacac agttttttca taaagcaagt tttaggttta
       61 cattaaatta aagtttaatt tgcgtggcta aggtattaaa ttatgtgcct gtttccaaca
      121 aatattttta gttttaatta acaaacacca cttttttttg tgaaaaatta gcatttaaca
      181 cttataacca tctggttacc tgcaagctaa gcaaactgtt aaattacagg gaggcaaaga
      241 aatttttttc aatatccgag agtatctcct cctccattgc aatcgttttc gatgcaattt
      301 ggttgcccct gtccaggtaa tccttaacca acgccttgtc cttcagcccg ctggagttga
      361 tttttacatt caggaatgct cccagcacag ccgatcgggc agccagcgcc cctacaccgg
      421 catcggaaac ggagcttgga ttgccctgcc gggccatagc cctgattacc tccagcgatt
      481 cgtagcaaag gttcatcacc ctaaagggaa cctctatggc ctgaatggtt gcttcctgaa
      541 ttgccttgct gcgtgcggcc ttctcgaggt cggttccctt gggaagcccg taggcctcca
      601 ttatccggtt aaaggctcgg gtatcctcgt caactagcgc tagcagctgc tccatgtagt
      661 attttccctt ttctgcccaa gttgaaaatt cctcccaccg gtcgtcccat ccgggcttgt
      721 gggccgaaag gtttgccacc atggcggcca gcgctgcccc aaaagcgccc atggccgccg
      781 aaatggaacc accgccgggc gcgggcgatt cggaggctgt ttcgcggcta aaactttcaa
      841 gtgtcattcc tgcaagctgc cttgtgccct tttcctcgag aatgtactcg ataatctttt
      901 tatcggggtc gaacgggtag agctcatcaa gccccatgga cttaacggca atccggatga
      961 tttccctgtc ggggatgccc accgatcgct gctgcttacg aaggaagtac ttcccggcct
     1021 ccagcatggc gctcaggggc acaacgccca caatctcgga gccggtaacc cgtaatccac
     1081 gttcctgagc ctttttactt gcctcctcaa aagccacatg caccggcgta accgttatgt
     1141 cagttaggtt gatggatacc tgtgcaatgc cgtactcctc aatgaaccag ccgatagcct
     1201 tgcatgcctt tagcgttccg ggaatccata tctctttccc attctcatcg gtggcaattt
     1261 taccggtaac agggttccca acccgcatgg gacgaccctt ctcacgaata tcgaatgcca
     1321 cggcattggc ccttcgggtt gaggtggtgt taaggttgac gttgtaggcc accaggaaat
     1381 tccttgcacc aacggcaata gcgccggttt tcgggagaaa ttgcgcaggg ccgaagtcgg
     1441 gtttccactc cggatcctgc agctttttag gaagtccctc atactctccc gccctgcagc
     1501 tggccaggtt gcgacgtttc tcctcaagcg cagcatactc atagcagtaa accggaatgc
     1561 ccagctcgtc gccaatcctt ttggccagcg cccgggcata ctccaccacc tcatccatgg
     1621 tgatgttggc cacaggtact agcgggcaaa catcggtggc gccaaaccgc ggatgtgcgc
     1681 cgtggtgcct gctcatgtca ataagctcct ttgctttggc aactgcccgg aatgccgctt
     1741 caaccacctc atcgggggtt ccaacaaagg ttacaaccgt acggttggtt gccttgccag
     1801 ggtcaacatc tataagctta acaccttcaa cagcttctat tacatcggtt atctgtttaa
     1861 ttaaaccact atcgcgcccc tcagaaaaat ttggaacgca ctcaattagc tttttctcgg
     1921 ccatttcagt atgaataaat tagatgtatg ataaataaaa taaaggataa aggataaagg
     1981 ataaaaaaca aaactcaaat cattcatttt tcttctcttt aaccccaaga cagtctcagg
     2041 acaaactttt aacttttttt cggttcgttg ttcacggttt cagcagacat tagtaatgtt
     2101 gccgttgaga aaaaccgttt caaccaggtc ggaggtgtac gcatagggca tgtactcaag
     2161 agtaggtatt ggttttgtaa taaagatatt ggctaccttg cccctgcaaa tgctcccatg
     2221 agtctcggaa agccccatgg catatgcacc gttgatggtt gtagcgttga ttacctcctc
     2281 aggaagcatt tttagcctaa tgcaacccaa cgacatgatg aacttcatgt cgcccgaagg
     2341 cgaagaaccg gggttgaaat cggatgcaag ggcaattgga agccctgcat caatcatttt
     2401 acgcacaggc gggttgtcca ttccaagaaa aaatgctgcc cctggaagca gggttggcat
     2461 ggtttcggac ccaagcaggg tttgaatttc atcatcacct gtaaattcca gatggtcaac
     2521 cgacaaggca ttgtacttta cgccaacctg tatgccacca gagtaatcga gctcattggc
     2581 atgaattttt ggtctcaggc cgtacttcat gccggccatc aggatgcgct cggtatcctc
     2641 gacggtaaaa aatcccctgt cgcaaaatac gtcaatatag tcggccaggt cctcggctgc
     2701 tacctggggt atcatctcgt tgataatctg gtcaacatat tcggcttgcc ggcctttata
     2761 ctcagcagga actgcgtggg ctcctaaaaa agtggcctta atggttagcg gtgttgcttc
     2821 gctaagcctg cggataaccc tcaacatttt cagctcattt tcggtgctca ggccgtagcc
     2881 gcttttaatt tcaacagctc ctgttccctt gcggataatt tctttaaccc taggcaatgc
     2941 ctgctcgtac agctcgtcct cggaagtttg ggagagtagc tttgcggagt taagaattcc
     3001 cccgccacgc ctggcaattt cctcgtacga tagccctttt atcttatcaa tatactcaac
     3061 ttcgcggctg ccggcatata ccagatgggt gtgagagtcg cagaaggagg gaaaaaccat
     3121 ccgcccattt acgttgtaaa tcagcacatc gctgggtagc ccggcaaatg aaggaacatc
     3181 cctcatagac ccaaaatcct tgattaaacc attctccatc agcaggaatg cgttatcaat
     3241 acacttcagg ttggccatat tttggcccgc aacccactga acaggctttt cctcgacctg
     3301 taccaacttt tttatgttaa gtagcagtat ttgcatggtt ttcagcataa atctccgacg
     3361 aaggtagcaa taaaattggg aagaactaaa tattatttaa taaaaggtat gcgttaaagc
     3421 cctgcaccag ccagcatcct agtgtttttg agctggtatt aaaaatttta ctcgttttgt
     3481 aacatttgtt acataattag gagggggtgg tgccttagct ttgctccaga aaaaggttga
     3541 aatttttttt cgtaaaaaag caggcatgaa aagagatatc attacaattg acagggaaaa
     3601 atgtaacggt tgcggccaat gtgtaggtgg atgccacgag ggtgccctgc agctgattga
     3661 cggcaaggca gttttagtta gcgagctgat gtgcgatggg ctgggagcct gcattggtga
     3721 atgccccgag ggagccatca ccgttgagcg gcgcgaggcc gatgcctacg atgaggtaac
     3781 caccctcagc caaatggtag ccagcggcaa aaataccaca gttgcccatc taaagcacct
     3841 taaagaatac caccaaaccg actatttacg acaagctgta gagtggctgc tttcaaatcg
     3901 taaaaatgtt tcctttagtg ttgatgaggt gatgcaggag gttcataacc actccggcag
     3961 cagctgtggt tgcggaaatg gagggaaaca tgaaccgaca caacagtccc accatggtaa
     4021 acatgtggat ggatgccccg gttcagcaga aaggagtttt ggaaataata tacgtgttga
     4081 ggcatcaaaa atggtatccg gcaagtcgga gctaacccat tggccggttc agctccatct
     4141 gataaatccg cattcggaac actttaaggg gtcgaacctg ctgctcgcag ccgattgtgt
     4201 agcctactcg caggggagct tccacagcca gcatcttgcc ggaaaaacgc tggccattgc
     4261 atgtcctaag cttgactcca acaaggagga gtacattgag aagattaccg cgtttattga
     4321 cgatgcccaa gtggacacca tcacagtgat gaagatggag gttccctgct gcggcgggtt
     4381 gctccagctt gcccggcttg cccgcgataa ggccaggcgt agtgtgccca taaaggtaat
     4441 aaccgttggc atacagggtg atattctgga ttccgtttgg gaaaactaag atggagaaaa
     4501 aactagagtc aagaataaaa tattttaaat ttacctgact cgagggtttt ccccatcaac
     4561 ctatttataa acaagctgca cagctactac ttacaaacaa tatctaacca atttaaaaat
     4621 tttgaatcat gagcatgttt tgttttcagt gtcaggagac agccaaagga accggctgta
     4681 caatcaaggg ggtatgcggt aagagcgatg accttgccaa catgatggac ctactaatgt
     4741 ttgttaccaa ggggatttgc gtttacagca ccgcactccg ggaacaaaag ggttatgaaa
     4801 accctatcgt gaacaagttt gttttcgaca gcctgttcac caccatcacc aacgctaact
     4861 tcgataagca ggtgatatcc aaccgggtat caagtgcgtt ggaaattcgg gaaacattac
     4921 gtaacgaagc ctcccttcaa ggagtaaggg ttgacacctc gtttgaggga tgcacatgga
     4981 gtggcacacc ggcagaatac gaagccaagg ccgccctggt aggagttctc agcgaaaaaa
     5041 atgaggatat acgctcgctc aaggagctga ttacatacgg ggtaaaagga atggcagcct
     5101 ataccgaaca tgcctacaac ctaggccatg aggatgtgca aaactacgcc ttcatgcagc
     5161 gggctttggc ctccataacc agaaatctga gcgtagatga gctagtggcg ctcaccctcg
     5221 aaaccggtaa gtttggcgta aacgccatgg cgctgctcga caaggccaac accagcagct
     5281 tcgggaatcc tgaaataacc aaggtgaatc tgggcgtaag gagcaatccc ggtatcctca
     5341 tcagcgggca tgatcttaag gatatggagg agctgctagc tcaaaccgag ggaacaggtg
     5401 tagatgttta cacccatagc gaaatgcttc cggccaacta ctaccctgcg tttaagaagt
     5461 acaggcactt tgttggaaac tatggcagca gctggtggca ccagcgtgag gatttcgaga
     5521 ccttcaacgg acccattcta tttaccacca actgtattgt gcccccttta aataatgcaa
     5581 cctataagga caaaatattt acaacgggtg cagcaggcta ccctggtagc aagcatgttg
     5641 ccgatcgcaa acccgggcaa caaaaggatt tcagcgaaat tattgctttg gctaaaacct
     5701 gtaaggcccc tacccaaatt gaaacaggcg agattattgg aggctttgcc cacgcccagg
     5761 tttttgcact tgccgataag gtggtagatg ctgttaaaag cggagcaatc agaaaattcg
     5821 tggtgatggc cggttgtgat ggtcgcatga aggaccgaac ctactacacc gagtttgctc
     5881 aaaaactacc caaggacacc gttattctca ccgctggatg cgccaagtac cgctataaca
     5941 agctagcttt gggcgacatc aacggaatac cccgggtgct ggatgccggg cagtgcaacg
     6001 actcctactc gctggccatc attgccctta agctgaagga gattttccag ctgaacgaca
     6061 tcaacgagct gccaattgcc tacaacatag catggtacga gcagaaagct gttattgtgc
     6121 tactcgccct gctctacctt ggggtaaaaa atatccattt aggcccgtca cttccgggtt
     6181 tcctttcacc gaacgtaacc aacgtgctgg ttgacaactt tggaataagc ggtataacta
     6241 ctgttgacga ggatattaag ctacttgtag ggtaattctt aaaatttcaa aaaaatacca
     6301 tcctgcgtgc ccgggtattc aagaaaccgc accaggatgg ttaattttaa acccttcaaa
     6361 aaaaatacag ccatgaacaa cgagtttgaa aaggaaaaga agttcaactt tctggatgag
     6421 gttgattaca gcacgggagg cgttgttagt aaaaacgtga ttaaaaaacc gacaggtaat
     6481 atttcgctgt ttgcattcga gaaaggagaa gggcttagcg agcatactgc accatttgac
     6541 gccctgctgc aggttattga cggcgaagct aaagttacca taaacggcaa gccacacgac
     6601 ctaaaggccg gagaatctat aatcatgcct gccaacagcc cccatgcagt ttttgcagct
     6661 gaacgcttca aaatggtcct taccatgatc aaggagtaaa ccatcatgaa gcctataaaa
     6721 aattcaccag cctgaacaca cgttcgggtt ggtggattct aaaaacattg taaggagc
//
LOCUS       DAOPHX010000125         6750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_14540_length_6750_cov_5.988499, whole genome shotgun sequence.
ACCESSION   DAOPHX010000125 DAOPHX010000000
VERSION     DAOPHX010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14540_length_6750_cov_5.988499"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..1616)
                     /gene="trkA"
                     /locus_tag="PK502_08885"
     CDS             complement(276..1616)
                     /gene="trkA"
                     /locus_tag="PK502_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HPH00969.1"
                     /translation="MNIIIVGAGEVGTHLATMLAKEYHDVTVIDPDPEMLSHVTAVSD
                     VITLEGSAASISILKEANVKKADLFIAVAHSEETNIVSATLAKRLGAKKVIARIDRYE
                     YLQPNNKEIFLNMGIDSLIYPERLAAREVITLLGQTSSTEFVDFSGGKLSLLVFRLEE
                     DSPVLDKTLFQATRSIQNLEYRAVAIAREGETIIPRGNDQFKLNDLVYVIANQAGIKD
                     MLEYTGKENIDINNLMILGGSRIAIHLAGELENSMNVKIIEVDREKSQQLAEQFKNAL
                     VINGDGRDTDLLMEEGMQYMDAFVAVTGNSETNILSCAIAKSLGVKKTIAEIENMSYI
                     RLAESIGVDTVINKKLITASRIFRYTMSTDVSAIKCLTGSDAEVLEFIVKPGSPVTKG
                     KLCEISFPKDAIIGGVVRGSTAFIAKGDSEIKPYDRVVVFALPSAINRVGRFFN"
     gene            complement(1705..3813)
                     /locus_tag="PK502_08890"
     CDS             complement(1705..3813)
                     /locus_tag="PK502_08890"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPH00970.1"
                     /translation="MSLPSSYHEVPFFRLLVPLVVGILVQLSLQPLHASMNILLLPVI
                     GFCILVVLSLLRKWELRWVYGLMLNLFMLVCGLVLSVNFTFNDDLSDLHEQGVVVRLT
                     ESPQLRARSLRAVGKVVRVMDKGSISPNEEKVLLYFSLTDSAAAQLQCGDIMAALLRF
                     STIEEPQNPYQFNFKQYMLARQIRFSSFVEPDSWARVGHHENGLVQLAGDLRRKLLAM
                     FSEFGLKDREYAVVSALTVGYTDLLDDALQKTYSTTGAMHILSVSGLHVGLVYGLLAF
                     LLSFLPKRAFFRIIKLPIQVVFLWFFALITGLSPPVCRSAFMFSLVAVGQGFNFRTNI
                     LNTLAAAAFALLVANPNNLLQVGFQLSFLAVLSIVVFHPFIYRLFTFKSKILDYFWSL
                     IAVSVAAQLGTIPVTLAAFGQFPTYFMLSNIIAIPLSTLILYAAVALVVFSPVPLVGA
                     FIGKILLVLTRSLNGGLQWIESLPHSSISGVYISRLQMLVLLSALVFISLYIVLKHSS
                     QLRAALLLMLFFFMLNIPQLRPVIRNELVVFHLPGSSMLCIRNNGLPIFIGTDSVGRN
                     LVEDSDFFLGGYISRQPSGRYGYINLCSPDWELLRQNGISLQRKYGLAVLEYNGRTVA
                     LPYSDSLRYATFGQTLNADVLVVNRFARGNLSDFIKPEVTVVDASVRFKHASAMLTRF
                     TGRGSAIHNLSVDGAYTAAL"
     gene            4182..4559
                     /locus_tag="PK502_08895"
     CDS             4182..4559
                     /locus_tag="PK502_08895"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPH00971.1"
                     /translation="MDKLIIESRIDTPKVVADGNTGLFEITGKSLPEDAFEFYKPLER
                     YVQGYIKQPQPKTTINLKLEYLNSSSSKKLLDVIGYFEQLSKHGYEIELNWYHHDEDQ
                     DMIDEGTEFANMTSLRVNFIAEH"
     gene            4732..6618
                     /locus_tag="PK502_08900"
     CDS             4732..6618
                     /locus_tag="PK502_08900"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPH00972.1"
                     /translation="MKKVVTILLILGLSVAPGFLPGQTIPAALQAEMEKNIELARSYE
                     TAGDINSALPLYTKTATTYWVNGNSEEARKLFIKAIALSERIGNTNALKMLLTNLGML
                     YVDEENYPEAIVNFEKCLSLNRKQNNKTEIASTLLNIANAQKELGQLQKSLNAAEEAS
                     KIAAELNDPKLMRNSYSLLSELYKEAGNDEKSAEYFTLYSAFSKKIQREEQLKKENEA
                     KKIVSEAQSQLHEIKTEKELTEQELMAKQHALTTVKDSLQKVEQLTREQRMEIDLLAK
                     ENALRDARIKNQILIRNIFIIAIILTLGIAGLITYYYLQKKRSNELLAHQKDMLEVKS
                     NELMEAIKQVEKQNRDITSSINYAQRIQQALLPTYNTLVTVINDSFIMLRPREIVSGD
                     FYWFAGYTGADSSTSTSNRHFIHVPSLKEDEGGFLIAAVDCTGHGVPGAFMSMIGLNL
                     IDNLVRTGRVRPDLLLGDLHKLIRYLLKQEESDNRDGMDIAICNIKNDGQTVEFAGAK
                     NPLFYVTNGQVNQVRGDPVPVGGLQKEKERQYTLHTIHIDTPTCFYIFSDGYIDQFGG
                     PYNQKFSSQQFKELLLKVHQLPMIQQQEILETTMDEWQGSENKQIDDIMVIGFRLNAK
                     PLGI"
BASE COUNT         2072 a         1626 c         1536 g         1516 t
ORIGIN      
        1 cttattaaat cggggatgtt ggttgccctg ctcgccatgg tactcgtagc tggttcgtgc
       61 agcaagaaaa cgtgtcctgc ctatagcgat acaaacaaag cacaggttat caacagggct
      121 taagtccgat ggttagcctg atttaggcat cagataataa tatcgtttaa aaaatatcct
      181 ctcgaacgct ttaggggtta cctgaacacc tgaagcgttt agttatacac ttaatgttag
      241 ttgggaaaac ttctacaagt ccaacccgaa aactgctagt tgaagaaacg cccaaccctg
      301 ttaatagccg aaggaagggc aaaaaccacc acccggtcgt agggtttaat ttcggaatcg
      361 cccttggcaa taaacgccgt gctaccccta acaaccccgc caataatggc gtccttggga
      421 aaagaaattt cgcaaagctt ccctttagta accggagaac ccggcttaac gatgaactcc
      481 agaacctcgg catcggaacc ggtaaggcat ttgatggcgg acacgtcggt gctcatggtg
      541 tagcggaata tgcggctggc tgtaatcagc tttttattga taaccgtgtc caccccaatg
      601 ctctcggcca accgtatata gctcatgttt tcaatttcgg caatagtctt cttcaccccg
      661 aggctttttg caatggcgca cgaaaggatg ttggtttcgg agttaccggt aaccgccaca
      721 aatgcatcca tgtactgcat accctcctcc ataagcaggt cggtatcgcg tccatcaccg
      781 ttaatcacca gcgcattctt aaattgttcg gccagctgct gactcttttc acggtccacc
      841 tctataatct taacattcat ggagttttcc agctcaccgg ccaggtgaat ggcaattcgg
      901 ctaccgccaa gaatcatcag gttgttgatg tcaatattct cctttccggt gtattcaagc
      961 atatccttaa tgccagcctg gttggcaatg acgtaaacca aatcgttcag cttaaactgg
     1021 tcgttacccc ggggaataat ggtttcacct tcgcgggcaa tggcaaccgc cctgtattct
     1081 aaattttgaa tgcttcgggt ggcctggaat agggttttat ccaaaaccgg ggaatcttct
     1141 tcgaggcgga aaaccagcaa cgatagcttg ccacccgaaa aatcgacaaa ttcggtggag
     1201 ctggtttgac ccaacagggt aattacctca cgggcggcta acctctccgg gtagattagg
     1261 gaatcgatcc ccatgttaag gaatatctcc ttattgttgg gctgaagata ctcgtagcgg
     1321 tcaatgcggg ctatcacctt tttagccccc aggcgcttag ccagcgtagc ggaaacaatg
     1381 ttggtctcct ccgaatgggc aacggcaata aacaaatcgg cttttttcac gttggcctct
     1441 ttgaggatac tgatggaggc agccgaacct tcgagggtta ttacatcgga aacagcggta
     1501 acatggctga gcatttcagg atcgggatca atcaccgtta catcgtgata ctccttggcc
     1561 agcatggtag caagatgcgt tcctacctca cctgccccaa caatgattat attcatgaca
     1621 ccctaaaaag aggttttagt gcatatttta cagctgcaaa gtaatactaa aaacagcaaa
     1681 cccattgcca ggttttcaat aattttagag ggcagctgta taggctccgt caaccgacag
     1741 gttgtggatg gccgaccccc tgccggtaaa cctggtaagc atcgccgaag catgtttaaa
     1801 cctaaccgag gcatccacaa cggtaacttc gggtttgatg aaatcggaaa ggtttccacg
     1861 ggcaaagcgg ttaaccacca gaacatcggc attgagcgtt tgcccaaatg tagcataccg
     1921 gagcgaatcg ctgtagggta acgcaaccgt tcgaccatta tactccagca cagccaatcc
     1981 gtattttcgc tggagcgata tgccgttttg tcgcagtagc tcccaatcgg gggagcacag
     2041 gttaatgtag ccatagcgcc ccgagggttg ccgggagata taccctccca ggaaaaaatc
     2101 gctatcctca actaaattcc gaccaaccga atcggtacca ataaatatgg gcagcccgtt
     2161 gttccgaata catagcatag agctgccagg aagatggaaa accaccagct cgttccggat
     2221 tacaggtcta agctggggaa tattcagcat gaagaaaaaa agcataagta aaagtgctgc
     2281 acgcagctgc gatgaatgct tcagcacgat atacagcgag atgaacacca acgccgagag
     2341 cagaacaagc atttgcagcc tggagatgta aactcccgat atggaggaat gcggcaggct
     2401 ttcaatccac tgcaatcccc cgttaagaga acgagtaaga acaagcagaa ttttgccgat
     2461 gaatgcccca accagcggaa caggcgaaaa aaccaccagg gctacagccg catacagaat
     2521 tagcgtggat aacggaatgg ctataatgtt ggatagcatg aagtaggtgg ggaattgccc
     2581 aaaagcagcc agggtaacgg gaattgttcc tagctgggca gccactgaaa cggcaatcag
     2641 cgaccagaag tagtccagaa ttttgctttt gaaagtaaac agcctgtaga taaaggggtg
     2701 aaaaaccacg atagagagca ccgccagaaa cgaaagttga aaccccacct gcagcaggtt
     2761 gtttggatta gctacaagaa gggcaaatgc tgcagcggcc agcgtgttta gaatattggt
     2821 gcgaaagtta aagccctggc caacggcaac cagcgagaac atgaaagccg aacggcaaac
     2881 aggcggggat agtccggtaa taagcgcaaa aaaccagaga aataccacct gaatgggtag
     2941 cttaattatc cggaaaaaag cccgtttcgg gaggaagctg agcaaaaagg ccagcaggcc
     3001 ataaaccagg ccaacatgca aaccggaaac ggaaagaatg tgcatggccc cggtggttga
     3061 ataggttttc tggagcgcat cgtcgagcag gtcggtgtag ccaaccgtta acgctgaaac
     3121 aacggcatac tctctatcct taagcccgaa ttcggaaaac atagccaaca gctttctacg
     3181 caagtctcct gccagctgaa caagaccatt ttcatgatgc ccaaccctgg cccagctgtc
     3241 aggctcaacg aagctgctga agcgaatctg gcgggcaagc atgtactgct taaagttgaa
     3301 ttggtagggg ttttgtggct cctctattgt gctgaaccta agaagggcag ccataatatc
     3361 cccgcactgc agctgagcgg cagcagaatc ggttaacgag aagtaaagca aaaccttttc
     3421 ctcgtttggt gaaatgctcc ccttatccat cacccgaacc actttcccaa ctgcccgcag
     3481 agaacgggca cgtagctggg gactttccgt caaccttact actactcctt gctcatgtaa
     3541 atcggaaagg tcatcattga aggtaaagtt aacggaaagc acaagcccgc agaccagcat
     3601 gaaaaggtta agcattaggc cataaaccca gcgcagctcc cattttctca gcagtgatag
     3661 caccaccaga atgcagaacc ctatcaccgg taaaagaaga atgttcatgc tggcatgaag
     3721 cggttgcagg ctcagctgaa cgagaatccc cacaaccagc ggtacaagca gcctgaaaaa
     3781 tggcacctcg tggtagctgg aaggtaacga cactgcagta gcaattaaac ttataaacaa
     3841 gttaatagaa ttccactgct tgtcaaaaaa aaaaaaatta atccaaaagg ctgataactc
     3901 ctcaagagca aaaaatgatt taacggaaaa atgcagctac taccatttta atcccttgag
     3961 aaataccccg cgaatggaaa aaggcaacac taggctttta ccaacataag gcagctgcgc
     4021 ggtataaatt ttaaataaaa accgtagggc agctgtagat taaacggcct gttattaatg
     4081 cagttaagaa ataagaacgg ccttagaata cagattatgc cctgcttatg gtaaaacctt
     4141 tggttacaat tttgcgtaaa tattgaaata aaatgagcaa catggataaa ctaataattg
     4201 aaagccggat agatactcca aaagttgtgg ctgatggcaa taccggcttg tttgaaatta
     4261 ccggcaagtc gctacccgaa gatgcctttg agttctacaa gcctctggaa aggtatgttc
     4321 agggatacat caaacagccc caaccaaaaa ccacaataaa cctaaagctg gagtacctca
     4381 actcctcatc ctccaaaaag ctgcttgatg tgattggtta ctttgagcag ctatcaaagc
     4441 atggttacga aattgagctt aactggtacc atcacgacga ggatcaggat atgatagacg
     4501 agggtacaga gtttgccaac atgacctccc ttcgggtaaa ttttattgcc gagcactagg
     4561 catacccatg cggttgtgca gcaagcactt agtagtaata tcacactaat ccagattttg
     4621 tcctgtaacc ggtgtataag gccctaacat ggctaacgca caagaaggca agtaggagtt
     4681 cgagttttca aaagcaaatt atttaggagc acacataata ccgaatatgt tgtgaaaaaa
     4741 gttgtcacca tacttttaat tcttggactt agcgttgcac ctggttttct ccctggacaa
     4801 accattcctg cagcccttca ggccgaaatg gagaaaaata ttgagttggc cagaagctac
     4861 gaaactgcag gcgacattaa ctccgcccta cccctttaca ccaaaacagc caccacctac
     4921 tgggtaaacg gtaattccga ggaggcaaga aaattgttta tcaaagcaat tgcgcttagc
     4981 gagcgtattg gaaataccaa tgccctcaaa atgcttttga ccaaccttgg catgttgtat
     5041 gttgacgagg aaaactaccc cgaggccatt gtcaactttg agaaatgcct atcgctcaac
     5101 cgaaagcaaa acaacaaaac ggaaatagcc tcaaccctgc taaacatagc caatgcccaa
     5161 aaggagttgg ggcagcttca aaaatcactc aacgcggcag aggaagccag caaaattgca
     5221 gccgagctga acgaccccaa gctgatgcga aactcatact cgttgctttc ggaactctat
     5281 aaggaagccg gcaacgacga aaaatcggct gaatacttta cgctctactc cgcattttca
     5341 aaaaaaattc aacgcgagga gcagcttaaa aaggaaaatg aggctaaaaa aatagttagc
     5401 gaagcccaaa gccagcttca cgaaataaaa acagaaaagg agcttaccga gcaagagttg
     5461 atggcaaaac agcatgcgct aacaaccgtt aaggacagcc tgcaaaaggt agagcaactc
     5521 acccgcgagc agcgtatgga gattgaccta cttgccaagg aaaatgcctt aagggatgcc
     5581 agaataaaaa accaaattct tatccgtaac atattcatca ttgccataat actcaccctg
     5641 ggcattgcag ggcttattac ctactactac ctgcaaaaga aacgctccaa cgagctcctg
     5701 gcacaccaaa aggacatgct ggaggtaaaa agtaatgagc taatggaagc cattaagcag
     5761 gttgaaaagc aaaaccgcga catcacgagc agcattaact atgcccagcg aattcagcaa
     5821 gccctgcttc caacctacaa tacgctggtt accgtgatca acgactcgtt cataatgctt
     5881 cgcccacgtg aaattgtaag cggcgacttt tactggtttg ccgggtacac cggcgccgat
     5941 tcgtcaacgt caacatcaaa ccggcatttt atacacgtac ccagtcttaa ggaggatgag
     6001 ggtggatttc taatagcagc ggttgactgc acaggtcatg gggtgcctgg tgctttcatg
     6061 tccatgattg ggctcaacct gattgacaac cttgtgcgaa cgggaagggt tcgccccgac
     6121 ctacttctgg gcgaccttca caagctaatc aggtatctcc tgaaacagga ggagagcgat
     6181 aacagggacg gcatggatat agccatttgc aatatcaaaa atgatggaca aacagttgaa
     6241 tttgccggcg ctaaaaaccc tctattctat gtaaccaacg gtcaggttaa ccaggttaga
     6301 ggcgatcctg tgcctgttgg tggactgcaa aaagaaaagg aacgccaata taccctccac
     6361 accatccata ttgacacccc aacctgcttc tacattttct ccgacggcta catcgaccag
     6421 tttggaggcc cgtataacca gaaattctca tcacagcaat tcaaggagct gctgcttaag
     6481 gttcaccagc ttcccatgat ccagcaacag gaaattcttg aaactactat ggatgaatgg
     6541 cagggaagtg aaaacaagca gatcgacgat ataatggtca ttggttttag gcttaacgcc
     6601 aaacctttag gcatatagca cccaaaagca atgctgtcgg ccatttcaca aacccgatgc
     6661 cttcatctgc tcccttgttg atctaaaaaa agtaattttt acgattaggg aattgagcaa
     6721 gaaaatatta taaatttgtc ccgacaaaga
//
LOCUS       DAOPHX010000126         6748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_14546_length_6748_cov_4.734947, whole genome shotgun sequence.
ACCESSION   DAOPHX010000126 DAOPHX010000000
VERSION     DAOPHX010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14546_length_6748_cov_4.734947"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PK502_08905"
     CDS             complement(<1..938)
                     /locus_tag="PK502_08905"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPH00973.1"
                     /translation="MMELRKWLLVFSTVFSSIVFAKSQEADSSSLTLLFVGDVMGHSP
                     QISSALNPLTGEYEYDSVFTRMKGVFSMADFTIANLEVTLGGEPYTGYPQFSSPDALV
                     DGLINAGVNVLVTANNHSADRGAKGIQRTVETLDRKGIIHTGTFSDSSDRLKNNPLIL
                     EKNGMRIALLNYTYGTNGLPVPAPCIVNKIDTLVIIRDIAMTRLMNADEIVVFIHWGN
                     EYERYPSAAQVAMANFLNRQGVRIVIGSHPHVVQPMEATLDTDSTTGRIVVYSLGNFV
                     SNQRRRYCDGGALAAVKLVKKSTGKSEITAAGFLPV"
     gene            complement(1014..1316)
                     /locus_tag="PK502_08910"
     CDS             complement(1014..1316)
                     /locus_tag="PK502_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00974.1"
                     /translation="MERKQRKYQEKAHQTLDEIFESIDSLDEKIRQASDKASSTMKEN
                     IEELKAEREKLKEKLKELNEASGESWKEIRNGFEEAATSLRESFIRAKNKFQDSEQ"
     gene            complement(1405..2481)
                     /locus_tag="PK502_08915"
     CDS             complement(1405..2481)
                     /locus_tag="PK502_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPH00975.1"
                     /translation="MPHINPDFADEIKKYGADDFNLCFNCGNCTAVCSLSTNDESFPR
                     EMIRYSTLGLEDEIKSSLKPWLCYYCGECSTDCPRQANPGELMMSLRRWLTAQYDWTG
                     LSGLLYKHIGAYIGGMLVLLAGVIGFTNFSGFDREQVIHYGHLFEMLSIAGVFAAILL
                     PNIIHMWYLTIVKPKVKPSLKVYAQSTGDLFVHMFTQKRFLGCEDTRENRTRWILHFI
                     LVLSYLSLLFTTVFLNWFSTTSKFVIWLGYFESAAIFIITTVFMSMRFRKVDTMHKHS
                     QPSDWFFVIWLFLMGITSFAVRLLIDLNNLDSQFWMYTLHLTVLAQWALIIVPFGKWT
                     HFLYRSFAMYFQKILELNRAVKRP"
     gene            complement(2487..4460)
                     /locus_tag="PK502_08920"
     CDS             complement(2487..4460)
                     /locus_tag="PK502_08920"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HPH00976.1"
                     /translation="MMKQRIGVYICHCGGNISDYVDVEQLGKLMAKEDGVIISKDVVF
                     ACSDSTQKDIEKDIKEMNLDAIVVASCSPKLHTHTFRGVAERAGINPYNYVQVNVREQ
                     CSWAHSDKPMDATIKAYGLIRAGINRVSHSEALEIIEIMAQKAVAVIGAGVSGLRAAI
                     DLARMGNQVYLIEKEAEVGGKVLTSGKLFMTGENGKEVIDRLYQRIKEMNNINIFTNA
                     NLENISGSFGNFMIDVNVAGELLKLNVGSVLVTTGFDFYQPKEGEYGYQLSDKVITLP
                     QFNKLINSNSGKLTYNGKTIKTIAYIYCVGNRQSKGENKYCSRQCCTAAIYSSLNVHE
                     KFEGIKAFHLYRDIRTYGKQELLYQESSRKGDVYIKFEEKEPPVIEVNEDKLSIKVKD
                     YLTSKKELMLDADLVVLVTGMVSRSDSSDISGKLKIPIGNDKFFNEIHPKLKPVETVI
                     KGIYIGGACQGPKNISESVQSALSGAAKINAAICKGSIELEPIVARINSETCIWCNKC
                     SEVCEFDAIKPIESNGKMIAEVNISSCTGCGICAPVCPTNAIEVAHFTDNEIESMIDG
                     FASPVNILPREHANSKTSSSKAQSLAGYPKIWRTIANALTEKPLTIPQIAEQINISVQ
                     DVTWNIMTMNRYGFIEPAGLDDDEAYYMYKLKK"
     gene            complement(4538..5869)
                     /locus_tag="PK502_08925"
     CDS             complement(4538..5869)
                     /locus_tag="PK502_08925"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HPH00977.1"
                     /translation="MPENKKTFDALVVGGGIAGQEAALSLANMDYKVLLVEKELSIGG
                     KMIQLSKVFPTLDCAACITTPKMSETARHPNIQLLLNTDIMEITKNNGLFNVNLTIQP
                     TYVNPDLCTGCQLCEWACPVVIVDKYNNNMVGQKAISIAFSLANPKVAAIDMNHCIHC
                     KKCEKVCEPKAIDYEMQPKNINIETKTVIIATGFNLFKGENKPQYGFKKFPNVIDAMQ
                     MDRLIAPTRPYNAVLRPSDRKVPDKIAYVLCTGSRDSSLENAIGCGAECSNNPICSQI
                     CCMYSIKQAQLLMGALPLADITIYYMDIRAFGKGYEEFFQQSKGMGVNFIKGKVAKIN
                     QKDDNSGDLILRYEDVTTGALKEANHDLVVLSVGVLPNNGITKVFTDDKLELDNQGFI
                     KSIDELVSPSLTSIDGVFVAGTAAGPKDIPDSILSAGSAASEAASYINSTL"
     gene            complement(5994..6422)
                     /locus_tag="PK502_08930"
     CDS             complement(5994..6422)
                     /locus_tag="PK502_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HPH00978.1"
                     /translation="MLSETFSPKILVFSTDIISDPGIDQAGLRKLHYPPTVYVISLPC
                     SSGIKPRWIMRAFEKGFDGVFIAADGHECSYSPKCAEHTNNIIVESQKLMKENNINPK
                     RIRMAALCSVCAEPFVNHMGNFSKILSELGSIGTEKYNPS"
     gene            complement(6511..>6748)
                     /locus_tag="PK502_08935"
     CDS             complement(6511..>6748)
                     /locus_tag="PK502_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HPH00979.1"
                     /translation="LKNLKVSQTVDARGTACPGPLLAAKKAIGEIESGEIMEVLSSDE
                     GTKHDIPKWCEKMEHEFLGIIEEDSFTRLFLKKA"
BASE COUNT         1759 a         1463 c         1388 g         2138 t
ORIGIN      
        1 catacgggaa gaaaacctgc agcagtaatt tctgatttac ctgtagattt cttaaccagc
       61 tttacggctg caagcgctcc cccatcacag tagcgtcttc gttggttcga cacaaagttt
      121 cctagggagt aaacaactat tctgccggtt gtgctatcgg tatctaaggt ggcttccatg
      181 ggttgaacta catggggatg agagccaatt actattctta ccccctggcg gtttagaaaa
      241 ttagccattg ctacttgcgc agccgatgga taccgctcat attcgtttcc ccagtggatg
      301 aacactacta tctcatcggc attcatcaac cttgtcatag caatatccct tattattacc
      361 aaagtgtcta tcttattgac aatacagggt gccggaacgg gcagtccgtt ggttccatag
      421 gtgtagttca gcagggcaat acgcatccca tttttctcga gaattaaagg gttattcttc
      481 aacctgtcgg atgaatcgga aaaggttccg gtgtgaatta tccctttgcg gtcgagggtt
      541 tcaactgttc gttgtattcc cttagcgccc ctgtctgcgg aatggttatt agcagtaacc
      601 agtacgttta ccccagcatt tattagacca tctacaagag catcgggtga gctgaattgc
      661 ggataccctg tataaggctc tccgcccagg gtaacttcaa ggttggcaat ggtgaagtcg
      721 gccattgaga atacgccctt cattctggta aaaacgctat catactcata ttctccggtt
      781 aaaggattaa gggctgatga aatttggggc gaatgcccca ttacatcacc cacaaaaaga
      841 agggttaggg agctactgtc agcttcctgc gatttagcaa aaactataga actaaacaca
      901 gtactaaata ccagcagcca ttttcttaac tccatcatca cattagaatt gctgaatagc
      961 cctgcaaggc aattttttcg ttctgcttta catgcccctt cgacaagatg aaactattgt
     1021 tctgaatcct gaaatttgtt tttggcacgt ataaatgatt cgcgaagcga ggtagccgct
     1081 tcctcaaatc catttcttat ctccttccat gactcgccac tggcctcgtt caattcctta
     1141 agcttttcct taagtttttc tctttctgcc tttagctcct ctatattttc cttcatggtg
     1201 ctgctggctt tgtcgcttgc ctgccttatt ttctcgtcaa gggagtctat actctcgaaa
     1261 atctcatcga gtgtctggtg tgccttttcc tgatattttc tttgttttct ttccatgggt
     1321 gaagtggttt aaatttatgc caatatagca ttttttagaa taatattacc agcgggcttt
     1381 ttgttgtttt gatgaggttt gcttttatgg ccttttaaca gccctgttaa gctccagtat
     1441 tttttgaaaa tacatggcaa atgaacggta tagaaaatgt gtccacttgc caaaggggac
     1501 tataatcaac gcccattgtg caaggacggt aaggtgaagg gtgtacatcc aaaactggct
     1561 gtccaggttg ttcaggtcaa taagcagccg tactgcgaat gaagtaattc ccattaggaa
     1621 gagccagatt acaaagaacc aatccgatgg ttgtgaatgc ttgtgcattg tgtcaacctt
     1681 tcgaaatcgc atactcataa aaacggtggt aataataaaa attgcagcac tctcaaagta
     1741 ccccagccag atgacgaatt tgctggttgt agagaaccag tttagaaaca cggtggtgaa
     1801 gagaagggag aggtagctca gaaccaggat aaagtgcaaa atccatcttg ttcggttttc
     1861 tctggtatcc tcacacccta gaaagcgttt ttgggtaaac atgtgcacga ataggtcgcc
     1921 agttgactgg gcataaactt taagggaagg ttttaccttg ggtttaacta ttgtaaggta
     1981 ccacatgtgg attatgttgg gtagaagtat agcagcaaaa actcctgcaa tggagagcat
     2041 ctcgaagagg tggccgtaat gtattacttg ctccctatca aaacccgaga aattggtgaa
     2101 acctatgact ccggcaagta aaactaacat acctccaatg taagcgccaa tgtgcttgta
     2161 gagcaagcct gaaaggcctg tccaatcgta ctgggcagta agccatcttc ggagcgacat
     2221 catcaattcc cccgggttgg cctgtcgggg acaatcggtg ctacactcac cacagtagta
     2281 gcacaaccaa ggtttaagtg atgattttat ttcatcctcc agcccaagcg tgctgtaacg
     2341 aatcatttcg cgaggaaacg attcgtcatt tgtggagaga gagcatacag cggtgcagtt
     2401 gccgcaatta aaacaaagat taaaatcatc ggctccgtat ttttttattt cgtcagcaaa
     2461 atcaggattt atgtgcggca tgataattac tttttgagtt tatacatgta gtaggcttca
     2521 tcgtcatcaa gtccggctgg ttcgataaat ccataccggt tcattgtcat aatattccag
     2581 gttacatctt gtacgctgat gttaatttgt tccgctattt gcggtatggt aagaggtttc
     2641 tcggtgagcg cattggctat tgttctccat attttagggt atcctgcaag gctttgtgcc
     2701 ttggaggagc ttgttttcga attagcatgc tctcttggta aaatgtttac cggtgaggca
     2761 aagccatcaa tcatagattc aatttcgtta tcggtgaaat gtgcaacttc aatagcgttg
     2821 gtagggcata ccggcgcaca tatcccgcat cctgtacagg atgagatatt tacctctgct
     2881 atcattttgc cattactttc aattggttta atggcatcaa attcgcaaac ctcactgcat
     2941 ttattgcacc aaatgcaagt ttctgagtta attctggcaa caatcggctc cagctctata
     3001 cttcctttgc aaatagctgc gttgattttt gctgcacccg acagtgccga ttgaaccgat
     3061 tccgatatat tctttggccc ctggcatgcc ccaccaatgt atataccctt aattactgtt
     3121 tctaccggtt ttagttttgg gtggatttcg ttaaagaatt tgtcgttacc aatgggtatt
     3181 tttagctttc cggagatatc gctactatcg cttcgcgaca ccattccggt taccaaaaca
     3241 actaaatcgg catctagcat taactctttc tttgaagtaa ggtaatcctt aacctttatt
     3301 gagagtttat cctcattaac ttcaataaca gggggttcct tttcctcaaa ttttatgtaa
     3361 acatctcctt ttctcgatga ttcctggtag agtagctcct gtttaccgta ggttcttata
     3421 tcccgatata ggtgaaatgc ctttatccct tcaaattttt catgaacatt cagtgatgaa
     3481 tagattgcag cagtgcaaca ttgacgtgag cagtacttat tttcaccttt gctctggcgg
     3541 tttccaacgc agtaaatgta ggctatagtt ttaatggttt tgccattgta agtaagcttc
     3601 ccagaattgg aattgataag tttgttaaat tgaggaagtg ttataacctt gtcggatagc
     3661 tgatatccat attcaccctc tttgggttga taaaaatcga aaccggttgt tactagaact
     3721 gagccaacgt tgagcttgag caattcgcca gcaacattca catcaatcat aaaattacca
     3781 aagcttcccg aaatattttc aaggttagca tttgtgaata tgttgatatt gttcatttcc
     3841 tttatgcgtt ggtatagccg gtcgataacc tctttaccat tttctccagt cataaatagc
     3901 ttgccagagg ttaatacttt tccgccaacc tcagcttcct tctcaattaa gtaaacctgg
     3961 ttgcccattc tagctaggtc aattgcagcc cgtaaacccg acactcctgc gccaattaca
     4021 gcaacagcct tttgtgccat aatttcgatg atttccaaag cctcggagtg cgatactcta
     4081 tttatgcctg cacgaattag gccgtaggct tttatggttg catccattgg tttatcggaa
     4141 tgagcccacg agcattgctc gcgcacgttt acctgaacgt agttgtaagg attaattcca
     4201 gcacgctctg caaccccgcg gaatgtatgc gtatgaagtt ttggcgagca cgatgcaacc
     4261 acaatggcat cgagattcat ttctttaata tctttctcaa tatctttttg agtagaatct
     4321 gagcaggcaa aaaccacatc ctttgatata attactccat cctcttttgc catcaacttt
     4381 ccaagctgtt ccacatcaac atagtcagag atgtttcccc cgcaatggca tatatatact
     4441 cctattcgtt gtttcatcat gctcaatgta tagtttgtta gtagtaatga gcgaggtgaa
     4501 gtatttattg attcttcgcc ttgctcagta tgacagctta tagtgtgctg ttgatatagc
     4561 ttgctgcctc cgatgccgct gaacctgcag atagtattga atcgggaata tcctttggac
     4621 ccgcagcggt acctgcaaca aatacaccat cgatactggt taacgagggg cttacaagtt
     4681 cgtcaatcga ttttataaag ccctgattat ccagctctaa tttatcatca gtaaaaactt
     4741 ttgttattcc gttgtttggt aaaaccccaa cggaaagcac taccaaatcg tgattagcct
     4801 ccttgagggc tccggttgta acatcctcgt atcggagaat taaatcgcca ctattgtcat
     4861 ccttttggtt gattttggca actttccctt taataaagtt gacccccatt ccttttgatt
     4921 gctggaagaa ctcctcgtag cccttgccaa atgcccttat atccatatag tatatggtga
     4981 tatcggccaa cgggagagca cccattagta gctgggcttg cttaatggag tacatacagc
     5041 aaatttgtga gcatattggg ttgttggagc actcagcccc gcatcctatg gcattctcaa
     5101 ggcttgaatc cctggagccc gtacaaagta cgtaggcaat tttatcggga accttacgat
     5161 cgcttggacg tagaactgca ttgtagggtc gagtaggagc aataagccta tccatttgca
     5221 tggcgtcaat tacgttggga aattttttaa atccatattg tggtttattc tcccctttaa
     5281 aaaggttaaa cccggttgca ataatcactg tttttgtctc aatgtttata ttttttggtt
     5341 gcatttcgta atcaatagct ttaggttcac aaaccttttc gcacttttta cagtgaatgc
     5401 agtggttcat gtcaattgca gctaccttgg ggttggctaa gctaaaggct atggatatgg
     5461 ccttttgccc aaccatattg ttgttgtatt tgtcaacgat tactactgga catgcccact
     5521 cgcatagctg acatccggta catagatcag ggttaacgta tgtaggttga atggttaggt
     5581 tgacattgaa taatccatta tttttagtaa tctccattat gtctgtattc agcagtaact
     5641 gaatgtttgg atgcctggcc gtttccgaca tttttggtgt agtgatacat gctgcgcagt
     5701 cgagggttgg aaacaccttg ctaagttgaa tcatcttacc accaatggaa agctcttttt
     5761 ccactagaag aaccttgtaa tccatattgg ctaaacttag cgctgcctcc tgacctgcga
     5821 ttccacctcc cactacaagg gcatcaaatg tttttttatt ttctggcatc gattaagatt
     5881 taaaataaat ctttaatttt ttgttgtgaa acgttgtgtg tacttagtgt tgattgtggt
     5941 taaattttta accgcaaagt ggctcaaagg atttcacgaa gtgcacgaaa gatttaactt
     6001 ggattgtatt tttcggtacc aatacttccc agttctgaga gtattttcga gaagtttccc
     6061 atatgattca caaatggctc agcacaaaca gaacataaag cagccatacg tattcgcttt
     6121 gggttaatgt tgttctcctt catcaacttc tgcgactcta caataatatt attggtgtgc
     6181 tcagcacatt ttgggctgta ggagcactca tggccatcag cagcaataaa aacaccgtcg
     6241 aaaccttttt cgaacgcccg cataatccat ctcggtttaa tgcccgatga gcagggcaag
     6301 ctaattacgt aaacagtagg ggggtagtgc agcttacgta atccggcttg gtcgatcccc
     6361 gggtccgata ttatgtcggt ggagaagacc agaatttttg gtgaaaatgt ttcacttaac
     6421 atgatgatag ctatgtttta tgcagaaaaa atattgttgt acaagtaaga agcaaggctt
     6481 aaaaagccct gcttccttat ccttttcacc ctaagccttt tttagaaata ggcgggtgaa
     6541 gctatcctct tcaataattc ccaggaactc atgctccatt ttttcgcacc attttggtat
     6601 atcgtgcttt gttccttcgt cggatgaaag tacttccatg atttcacccg attcaatttc
     6661 tccaatagct ttttttgcag ccaaaagggg acctgggcaa gccgtacccc gtgcatcaac
     6721 ggtttggctt acctttagat tttttaat
//
LOCUS       DAOPHX010000127         6719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_14628_length_6719_cov_7.377251, whole genome shotgun sequence.
ACCESSION   DAOPHX010000127 DAOPHX010000000
VERSION     DAOPHX010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14628_length_6719_cov_7.377251"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..2476)
                     /locus_tag="PK502_08940"
     CDS             complement(260..2476)
                     /locus_tag="PK502_08940"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPH00980.1"
                     /translation="MIISNVFWVVPIASVLALLFAYYFFRQMMKEDEGTDIMKKIALH
                     VRKGAMSYLKQQYKVVGLVFVVLVLLFALLAYGLHVQNGWTPFAFLTGGFFSGLAGFL
                     GMKTATYASARTANAAQKSLNRGLKIAFRSGAVMGLVVVGLAVLDISLWYLILNYFID
                     EPDPSQKLIVITTTMLTFGMGASTQALFARVGGGIYTKAADVGADLVGKVEAGIPEDD
                     PRNPATIADNVGDNVGDVAGMGADLYESYAGSILATAALGASAFALFEGNMQYKAILA
                     PMLIASVGIVLSLLGIFIVRTKENATMRDLLRSLGRGVNVSSLLIALSTFGVLYGLGL
                     HHWLGISFSVVSGIAAGVIIGQSTEYFTSHSYKPTQRVSESSKTGPATVVISGIGLGM
                     VSTAIPVITITLAILMAFLCAINFDVKHMLEAQNIQLGLYGIGIAAVGMLSTLGITLA
                     TDAYGPIADNAGGNAEMSHLGPEVRKRTDALDALGNTTAATGKGFAIGSAALTALALL
                     ASYIEEIKIGLVRIGENVLTFANGETVETAKATIVDFMSYYQINLMNPIVLAGIFIGA
                     MMAFLFSGLTMNAVGRAAQKMVEEVRRQFREIKGIMTGETEPDYERCVAISTRGAQIE
                     MILPSSLAIIVPILTGLLLGVAGVMGLLTGGLGAGFVLAIFMANSGGSWDNAKKYIEE
                     GNFGGKGSDCHKAAVVGDTVGDPFKDTSGPSLNILIKLMSMVSIVMAGVTVAFSIF"
     gene            complement(2701..4239)
                     /locus_tag="PK502_08945"
     CDS             complement(2701..4239)
                     /locus_tag="PK502_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPH00981.1"
                     /translation="MLVKTFGSAVHGIKATTITIEVSVSQGINFFLVGLPDNAVKESQ
                     QRITSALETNGYRFPKKRVVINMAPADIRKEGSAYDLPLALGILAASEQVNPQMLPDY
                     VVMGELSLDGSVQPVRGVLPIAIQAREEGFKGFIVPIQNAREAAVVNDLDVYGVSSIR
                     EVIDFFNGNESLKPTIINTREEFFANANSYDVDFADVKGQENVKRALEVAAAGGHNII
                     MIGPPGAGKTMLAKRIPTIIPPLTLREALETTKIHSVAGKIDRDTSLMTRRPFRSPHH
                     TISDVALVGGGQYPQPGEISLAHNGVLFLDELPEFKRTVLEVMRQPLEDRVITISRAK
                     FSVEYPASFMLVASMNPCPCGNYNNPFKECVCSPGMVQKYLNRISGPLLDRIDIHIEV
                     IPVPFEKLATTINSETSETIRKRVIKARQVQEERFVEWKEVYCNAQMTSKLIRKFCEL
                     DSAGSSLLKTAMERLNLSARAYDRILKVSRTIADLDDSPSIKPEHLAEAIQYRSLDRE
                     NWAG"
     gene            4650..5504
                     /locus_tag="PK502_08950"
     CDS             4650..5504
                     /locus_tag="PK502_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPH00982.1"
                     /translation="MKISQFNISNFKVDGGAMFGVVPKTMWGKVYPSDENNLIPLSLK
                     SLVVEANGRVVLIDNGIGSKQDAKFMSHVYAFGGEGLLPGLAARGYRPEDVTDVILTH
                     LHFDHCGGGIFRDSHGKFQLTFPNALYHVSAAQWEWATHPNSREDDSFLPENILPMKE
                     LGALHLIESEGELIPGLSLRIFNGHTRGQLIPLISYRGKTLVFCADLFPFTAQIPLPW
                     IMSYDVEPLKTLQEKEQFLNEALRGNYILLYEHDYFADCSRLQLTAKGIRATEGFSFQ
                     QLLKELNE"
     gene            complement(5484..5915)
                     /locus_tag="PK502_08955"
     CDS             complement(5484..5915)
                     /locus_tag="PK502_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00983.1"
                     /translation="MKPPSCKKGINVYASVNALFIVVILAVFMYSFAFKTEKYPIPAL
                     LTQSTGLIPPSRGLSKSFSEIVRGNISAAKLINPHSLQIFAFFLIQLVMRVAGFAAAK
                     IIGSPFPVKGIAVDVCISLALFVFCFAPLISYTFRLFVQLF"
     gene            complement(5921..6493)
                     /locus_tag="PK502_08960"
     CDS             complement(5921..6493)
                     /locus_tag="PK502_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00984.1"
                     /translation="MDVETRTTAGQVMGVIGIVLGVVSLVLAFIPCIGVMAFIPGVLA
                     LTFSIISIVQASNGYGSKGLGIGALIVSLVSIVLAAAWLIVFSGTFAFADRAIRNSDK
                     IEIFGKEFGKAFEEEFGKGDVNLKIVSDSLENSLRQLENELDSLDGYIPEEKARKAGQ
                     AAARALKKSTRVVIETDSGKIQFETSNKSH"
BASE COUNT         1805 a         1565 c         1562 g         1787 t
ORIGIN      
        1 cctcatcggc cacccccgaa actgatttta tacagcgttc aatgcaatcc tcctcgttgt
       61 aagctatgat tacggctgaa atttgggtca tgttattttg aaataattct gacaaatggc
      121 gtgtaaaagt aagatttttt caataacacg gttgctggtt taacgaattt aattgccatc
      181 aggttagagg ggaagcaggg ggtagagaga aaaaagccca gctggatatg ctgggctttt
      241 aaaggattca tggctattcc taaaaaatgc tgaaggccac tgtaaccccc gccataacta
      301 tggacaccat gctcatcaac ttgatgagaa tgttgaggct tgggcctgag gtatccttga
      361 atgggtcgcc tacggtatca ccaaccacgg cagccttatg gcaatcggaa cccttacccc
      421 cgaagttccc ctcctcgatg tatttttttg cattatccca agaacctccc gagttggcca
      481 tgaaaatggc cagcacaaat cctgccccaa gtccgccggt aagtaagccc atcacgccag
      541 ctacacccag cagcaaaccg gtaagtattg gtacaataat agccaatgaa gagggaagga
      601 tcatttcgat ttgcgctccc cgggtagaga tggccacaca ccgctcgtaa tcgggctcgg
      661 tttcccctgt cattatccct ttaatttccc tgaactgcct gcgaacctcc tccaccatct
      721 tctgggcagc gcgaccaacc gcattcatgg tcaaccccga gaagaggaat gccatcatgg
      781 ctcctatgaa aattccggcg agtacaatag gattcatcag gtttatctgg tagtagctca
      841 taaaatcaac aatggttgcc ttggcagtct caacggtttc accattggca aaggtgagaa
      901 cgttttcacc tattcgcaca aggccaatct taatttcttc gatgtacgaa gctagcaggg
      961 ccagcgctgt tagggcggcc gaaccaatgg caaaaccttt accagtggca gcggtagtgt
     1021 ttcccaatgc atcaagggca tcggtacgtt tgcggacttc agggccaagg tgactcatct
     1081 cggcatttcc ccctgcatta tcggcgattg gcccgtaggc atcggttgcc agggttattc
     1141 ctaaagttga cagcatcccc acggcggcaa tgccaattcc atagagcccc agctggatgt
     1201 tttgagcttc cagcatatgc ttaacgtcaa agttgatggc gcataaaaat gccatcaaaa
     1261 tggcaagggt tatggtgata accggtatgg ctgttgatac cattcccagg ccgatgcccg
     1321 aaatgacaac ggtagccggt ccggtttttg aactttcgga taccctttgg gtgggcttgt
     1381 acgaatggga ggtgaagtac tcggtggatt gcccaatgat tacacctgct gcaattcccg
     1441 atacaaccga aaatgaaatt cctagccagt ggtgaagccc aagtccataa agaactccaa
     1501 aagttgagag ggcaattagc agcgagctta cgttaacacc cctgccaagc gaacggagca
     1561 aatccctcat ggtggcgttc tccttggtgc ggacaatgaa aatgcccagc agcgatagca
     1621 caataccaac cgacgcaata agcatgggag ctaaaatagc tttgtactgc atgttacctt
     1681 caaatagtgc aaatgctgat gcccctagtg cagcagtagc aaggattgaa cctgcatagg
     1741 attcgtacag gtcggcaccc attccggcta catcgcccac gttgtcgcct acattatcgg
     1801 caatggtggc agggttacgg ggatcatcct cgggaatgcc ggcttcaacc ttaccaacaa
     1861 ggtcggcgcc aacatcagca gccttggtgt aaattccacc tccaacacgg gcaaatagcg
     1921 cctgggtgga tgctcccata ccaaaggtaa gcatagtagt agtgatgaca attagcttct
     1981 gggatgggtc gggctcgtct ataaagtagt tgaggataag ataccatagc gaaatgtcga
     2041 gaaccgccag tccaaccacc accagcccca taacggcacc cgagcgaaaa gcaattttca
     2101 gacctcggtt aagcgatttt tgtgcagcat ttgctgtgcg ggccgatgcg taagtggcgg
     2161 ttttcatacc caaaaatccg gccaagcccg aaaagaagcc gccggtaaga aatgcgaatg
     2221 gcgtccatcc attttgaacg tgaagaccat aggccagaag tgcgaagagg agaacaagca
     2281 ccacaaaaac aagcccaaca actttatact gctgttttag gtacgacatg gcgccctttc
     2341 gcacatgaag ggcaattttt ttcatgatat ccgtaccctc atcctccttc atcatctgcc
     2401 tgaaaaagta gtaggcaaat agcagggcaa gcaccgatgc aatcggaaca acccagaata
     2461 cattggaaat tatcatacta gttgttttta ggttgaatta tagttaggtt ataaatcaga
     2521 aattcaacaa tatgcaagta tggtttagca acaaatccct tttattttta aactcgccaa
     2581 tatacaaagt gttgaatggt aatgaaagca tatttggttt tttttcgggt tgactcgaag
     2641 tgtttttatt caacttttag taattgggag agtttaaaaa atagcataag ataaaagcct
     2701 ttaccctgcc cagttttctc tgtcgaggct tcggtactgt atggcctcgg ccagatgctc
     2761 gggtttgata ctgggtgaat catccaaatc ggcaatcgta cgggaaactt ttagtattct
     2821 atcgtaggcc cttgctgaga ggttgagccg ttccatggca gtttttagca gcgacgatcc
     2881 agcactgtcg agctcacaga attttctgat cagcttggag gtcatctggg cattgcagta
     2941 aacttccttc cactctacaa atcgttcttc ctgaacttgg cgtgctttaa ttaccctttt
     3001 acggatggtt tcgctggttt cggagtttat tgttgtggcc agcttctcga acggtacggg
     3061 aataacctct atgtgtatgt ctattctatc gaggaggggg ccggaaattc ggtttaggta
     3121 cttttgcacc atgcctggtg agcaaacgca ttctttaaaa gggttgttat agtttccgca
     3181 tggacagggg ttcatggagg caaccagcat gaagctggcg gggtactcca cggaaaattt
     3241 tgctctggat atggtgataa ccctatcctc gagcggttga cgcatgacct ccagcacggt
     3301 tcgtttaaat tctggcagct cgtcgaggaa tagcacgcca ttatgagcca gcgaaatttc
     3361 gccgggttgg gggtactgtc caccaccaac cagcgctaca tctgagatgg tgtggtgagg
     3421 gctacggaaa ggacgtcggg tcattagaga ggtgtcgcgg tctatctttc cggcaacaga
     3481 atgaattttc gtggtttcga gcgcttcgcg cagcgtaaga gggggaatga tggttggaat
     3541 ccgtttggcc agcatggttt taccggcgcc aggaggccca atcatgatga tgttgtgccc
     3601 accggcagcg gcaacctcca gcgcacgctt cacattttcc tgccccttta catcggcaaa
     3661 gtcaacatcg tagctgttgg cgttggcaaa aaattcctca cgggtattga taattgtggg
     3721 ctttaggctt tcgtttccgt tgaaaaaatc aatgacttcc cgaattgatg ataccccgta
     3781 aacatccaaa tcgtttacta cggctgcttc ccgtgcattt tgtatgggca cgatgaaccc
     3841 tttgaagccc tcctcacgag cctgaatggc aatgggaagc acccccctta cgggctgaac
     3901 ggagccatcg agcgaaagtt cgcccataac aacgtaatcc ggcagcattt gagggtttac
     3961 ctgttccgat gctgccagaa tccccaatgc cagtggtagg tcgtaggccg agccctcctt
     4021 ccttatatcg gccggagcca tgttgataac aacccgtttc tttgggaacc tatatccatt
     4081 tgtttctaaa gccgaggtga ttcgttgctg gctttccttc accgcattgt ccggaagccc
     4141 tacaagaaag aagtttatcc cttgcgaaac actcacctca atggttatgg tggtggcctt
     4201 tatgccatga acagcacttc caaaagtctt tacaagcata tatgccaaaa tttatatcac
     4261 ttgcaattta ctcgaaatta atgaatagcg gaaatacgaa gtacctttat gctcataagt
     4321 gtagccaaaa atttattggc gcctgttgat tttgccttaa gcagattttt tatcaaacag
     4381 tttttgtaca gaaatgcact taccctttga atttttccca gattccctaa ttcaatggtt
     4441 gagttttaac caatcaactt gacaataaat gctgcaagta gagtatgctt tgcccattta
     4501 cttgttgggg agctcatttt ttatttcaga gtgaacggga atgaactata tttcgccagc
     4561 tatggcatta agcgctatgt tgtgcccgag gagtttgtgt tgcgtgttac aggaattgta
     4621 actttgtaaa atattttaac tcaacatgta tgaagatttc gcagtttaac atatcaaact
     4681 ttaaggttga cggtggggca atgtttggcg tagttccaaa aactatgtgg ggtaaggttt
     4741 acccctctga tgagaataac ctgatacccc tttcgcttaa atcgctggtt gttgaggcta
     4801 acgggagggt tgtgctgatt gacaatggga taggcagtaa gcaggatgca aagtttatga
     4861 gccatgttta cgcttttggc ggcgagggct tgttgcccgg tttagcagca aggggctaca
     4921 ggccagaaga tgttaccgat gtgattctca cccacctgca ttttgaccac tgcggtggag
     4981 ggattttccg tgattcccat gggaaatttc agctgacatt ccctaatgcc ctttaccatg
     5041 tttcggccgc acaatgggag tgggctaccc acccaaactc ccgggaggac gattcctttc
     5101 ttcctgaaaa tattcttcct atgaaggagt tgggtgcact acacctgata gaatccgaag
     5161 gagaattgat acccggactt tctcttagaa tttttaacgg ccacacccgc ggacagctta
     5221 ttccgctaat cagctatcgt gggaaaacgc tggtattctg tgccgatctt ttccctttca
     5281 ctgcccaaat tcctttaccc tggattatga gctacgatgt tgaaccgctt aaaacgcttc
     5341 aggaaaagga gcagtttctc aacgaggcgc ttcgggggaa ctacatttta ctgtatgaac
     5401 acgattactt tgccgattgc agccgccttc agcttactgc taaaggaata agggcaacgg
     5461 agggtttttc gtttcagcag cttctaaaag agctgaacga atagcctgaa agtatatgaa
     5521 ataagcgggg caaagcagaa gacaaaaagc gctagcgata tgcaaacatc tactgcgata
     5581 ccttttacgg gaaatgggct gccaataatt ttagctgctg caaacccggc aacccgcatg
     5641 accagctgaa tcaaaaaaaa tgcgaatatc tgcaaagaat gagggttgat aagctttgct
     5701 gcggatatat ttccccttac aatttcagaa aaactttttg aaagtccccg gctcggaggt
     5761 attaaccctg tactttgggt gagcagtgca ggaattggat atttttcggt cttgaacgca
     5821 aaggagtaca taaaaactgc caggataacg acaataaaaa gtgcattaac agaagcgtaa
     5881 acgttaatac cctttttgca agacggaggt ttcaactact ttaatggctt ttattgctgg
     5941 tttcaaattg aatcttaccc gagtcggttt caattaccac ccgtgtagat ttttttaagg
     6001 ctctggcagc agcttgtcct gccttgcgag ccttctcttc cggtatgtag ccatccagac
     6061 tgtccagctc gttttcgagc tggcgaagtg agttttcgag gctgtcggaa acaattttta
     6121 agttcacatc tcccttgcca aactcttcct caaaggcctt gccaaattcc ttgccaaaaa
     6181 tttctatctt gtcgctattc ctaattgccc tgtcggcaaa ggcaaatgtg ccactaaaga
     6241 caataagcca tgcggcagca agcacaatag ataccagcga aacaatcaag gcacctatac
     6301 ccaggccctt agacccataa ccattcgaag cctgcacaat agagattatg ctgaaagtaa
     6361 gggccagcac tcccggaatg aaagccataa cgccaataca tggtataaag gccaagacca
     6421 gcgaaacaac tcctaacact atcccgataa cacccataac ctgtccggct gtggttcttg
     6481 tttctacatc catattctgg tttttttagt tatacaaaaa tgacgaaaat ttaagtcatc
     6541 cgctgcattg agcacaataa aaactactgt ttttgatcca aaaaaaaatt ctggaggagt
     6601 agataatact actttaggcc atgacctaag ggtttagggt agagttttta ttttcaaagg
     6661 ggatgggcat gatttttttc ccgatgctgc aatataaaaa gcagggagta gaagctgaa
//
LOCUS       DAOPHX010000128         6618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_14980_length_6618_cov_5.691147, whole genome shotgun sequence.
ACCESSION   DAOPHX010000128 DAOPHX010000000
VERSION     DAOPHX010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14980_length_6618_cov_5.691147"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2389
                     /locus_tag="PK502_08965"
     CDS             <1..2389
                     /locus_tag="PK502_08965"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPH00985.1"
                     /translation="PHVIAVLVFMAISLAYFYPQIQGKRLYQHDIAQWRGSAKEIMDF
                     KEKTGKETLWTNSMFGGMPAYLISANYSGNLMLKVNKAMNFLQRPASFIFIAMLGFYI
                     LLLVFGLNPWLSLVGALGYGLSTYFFIIIGAGHNSKIQAIGYVAPMIAGLVMACKGRF
                     WGGMAIFGFFFGLNLASGHPQITYYSAFIMLALLVAFFINAQKTKTLKTIYKAIGFLA
                     IAGILAVGSNFSNLWFTYDYGKDSIRGKSELTDNQHNKTSGLDKDYVTQWSYGVAETF
                     NMLIPNLMGGASSMDVGENSETFKFLRQNGVPFSQAQTIVKQLPTYWGPQPSTSGPVY
                     VGAIIFFLFILGMFLLRGPEKWALFAVTLLAILLAWGHHFNFLTNFFLNYFPAYNKFR
                     TVSMILVVAELTMPLIGFLGLKEIYEGKIAKPDFLRAFKWATGITAGICLFFLIFGRG
                     IFNFSGAVDQQLGWPKELIDVLKRDRQHMLTNDSLRSLFFVLAGAGIVYAFFIKKIKP
                     SYFLLSLGLLITIDMWVVNKRYMNNDGFVNKRMVEAPFTPTDADKQILADKSPDFRVF
                     NLTVSPFNDASTSFYHKSIGGYHGAKLRRYQDLIDYHLSKGNMAVLNMLNTKYFIQPG
                     QNGPIALPNPDALGNVWFVDSIKLVNNPDEEIQALYNFNPKTTAIVDKKFESLLADFK
                     PDNSGAGNIELVEYAPNMLKYHSISNQPKLAVFSEIYYPKGWKASIDGKPAPHFRANY
                     VLRAMIVPAGEHRIEFTFDPPMWRVGKSIDLTSSLLLILIFVGWIGWSAFSKKSI"
     gene            2407..3285
                     /locus_tag="PK502_08970"
     CDS             2407..3285
                     /locus_tag="PK502_08970"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPH00986.1"
                     /translation="MKNQTKKSAKGRLRNSYISSIISISLVLFTLGILGLLVISSQKL
                     SDYMKENIGFTIYLNDSVSNADANYLKKLLDASTYVRSSQYYSKEDAASLMEKELGED
                     FISYLGYNPLSAFIDVRLKADYANNDSIPKIESWLKQFQQVKEVDYQKSLVNLVNENV
                     SRLGLVVLAFGALMLFIALVLINNTIRLSVYSRRFLINTMKQVGATWGFIRKPFLLRS
                     ILHGLYASIIAIGLLSGLIYGIRNEVADILQIVEPYYIMGIFAAVTLVGIAINLTATL
                     LAVNKFLRLNADDLYY"
     gene            3322..3591
                     /locus_tag="PK502_08975"
     CDS             3322..3591
                     /locus_tag="PK502_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HPH00987.1"
                     /translation="MAKNANKPADSSQVEGKFALGKENYKLLLIGFGIIILGFILMAG
                     GGSKDPNVFDPRIFSFRRITLAPIVVLGGFVFEIYAIMKKPKHKS"
     gene            3919..4632
                     /gene="truB"
                     /locus_tag="PK502_08980"
     CDS             3919..4632
                     /gene="truB"
                     /locus_tag="PK502_08980"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPH00988.1"
                     /translation="MDVNNFNATKEGTAILVDKPLGWTSFNVVGKIRTLIHRATGDRT
                     IKVGHAGTLDPLATGLLVICTGRATKQIDSLMNGKKEYLATLKLGETTPSFDLETPPD
                     GQFPTEHITPTLIAETLKNFMGIQHQTPPLFSAKYINGKRAYKYARKGIEVALEPVPI
                     EIFEMEMLSFEPPLLTLRICCSKGTYIRALARDIGVALGSGAHLVALRRTVSGDFRIS
                     EALSIQELEKIFSSPMQQT"
     gene            4763..5812
                     /gene="queA"
                     /locus_tag="PK502_08985"
     CDS             4763..5812
                     /gene="queA"
                     /locus_tag="PK502_08985"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPH00989.1"
                     /translation="MKLSQYKYNLPQDLIAKFPTENRDESRLMVLNRKNHKIEHRIFK
                     DVIDYIDENDVVVFNNTKVFPARLYGNKEKTGAEIEVFLLRELNREQRLWDVLVDPAR
                     KIRIGNKLYFGDDDILVAEVIDNTTSRGRTLRFLFDGPYEKFKETLYKLGETPLPKFI
                     RREVVPEDRERYQTVYAKHEGAVAAPTAGLHFSKQLLKRMEIKGINFAEITLHVGLGN
                     FRSVDVEDLTKHKMDSEQINIPEEAAEIINRAKQNHHRVCAVGTTVLRTLESSVTTSG
                     QLKPFSGWTNKFIFPPYEFSIPDMLITNFHLPQSTLMMMVSAFSGYDFLMEAYKLAIK
                     EKYRFGTYGDAMLII"
     gene            complement(5953..>6618)
                     /locus_tag="PK502_08990"
     CDS             complement(5953..>6618)
                     /locus_tag="PK502_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00990.1"
                     /translation="GSGEYVCMMANRLKETFGEASIIIFIRNQHSALESVYGHYIKSG
                     GTYSVKKYMGIYRKFTRPFHDYHLFNPDLFQYNKIVELYARLFGSENVHVCLFEDFKA
                     NPEGFIDQFSRDFNLSTSTHLNFEPQNIKLSTFSVSLMRFSNHFTLKNTVFKHYFFSI
                     PRLYRVLLSVTQMLDKSFLTLHPFTFDKDVHSWIDERFRMGNRELARWVNMEKLREYG
                     YPF"
BASE COUNT         1840 a         1553 c         1469 g         1756 t
ORIGIN      
        1 accccatgtt attgccgtgc tagtgtttat ggcaatttca cttgcctatt tttatcctca
       61 aattcagggc aaaaggctct accagcacga tattgcccag tggaggggat cggctaagga
      121 aattatggac ttcaaggaaa aaacggggaa ggaaacccta tggaccaaca gcatgtttgg
      181 aggaatgccc gcctacctga tttcagccaa ctattcaggc aacctgatgc tgaaggttaa
      241 caaggcaatg aattttctac aacgtccggc cagcttcatt tttattgcta tgcttggttt
      301 ctacatcctg ctgcttgttt tcggattaaa tccttggctg agtttggttg gtgcgctagg
      361 ctacggccta tcaacatatt ttttcatcat aattggagca gggcataact ccaaaattca
      421 ggcaattggc tatgttgcac ccatgattgc aggactggtt atggcctgta agggaaggtt
      481 ctggggcgga atggccatat ttggcttttt ctttgggctt aacctggcaa gtgggcatcc
      541 acaaattacc tactacagcg catttatcat gctggccctg cttgttgcgt tcttcataaa
      601 tgcccaaaag accaaaaccc tgaaaactat ctataaggcc atcggatttc ttgcaattgc
      661 aggaattctt gctgtgggat caaactttag caacctatgg tttacctacg attacggcaa
      721 agactccata agggggaaat ctgagctaac cgacaaccag cacaacaaaa cctcgggact
      781 cgacaaggat tacgttaccc agtggagcta cggagttgcg gaaaccttca acatgctaat
      841 ccccaacctt atgggaggcg ccagctccat ggatgttggc gaaaactccg aaacattcaa
      901 gttcctgcgt caaaatgggg ttccctttag ccaggcgcaa acaattgtga agcaactgcc
      961 aacctactgg ggaccacaac cctcaacctc gggacctgtt tacgtgggcg ccatcatttt
     1021 tttcctcttc atactaggca tgtttcttct tagaggcccc gagaaatggg cattgtttgc
     1081 agttaccctg ctggcaatcc tactagcctg ggggcaccac ttcaacttcc ttacaaattt
     1141 ttttctcaac tactttccgg cctacaacaa attccggaca gtctcgatga ttctggtggt
     1201 tgccgagcta accatgcccc taataggttt tctgggctta aaggagattt atgagggaaa
     1261 aattgctaaa ccagactttc tcagggcatt taaatgggca actggtatta ccgcaggaat
     1321 ttgcttgttt ttcttgatat ttggaagggg catttttaac tttagcggag ctgttgatca
     1381 gcagcttggc tggccaaagg aacttattga tgtcctaaaa agagaccggc agcacatgct
     1441 gactaacgac tccctccgtt cgctattttt tgttcttgcc ggagcgggca ttgtttacgc
     1501 attttttatc aaaaaaataa aaccttccta cttcctgctt tccttaggcc tgctcattac
     1561 aatagatatg tgggtggtta acaagcgcta catgaacaac gatggctttg ttaataaacg
     1621 aatggtagaa gcccctttta cccccacgga tgccgataag cagattttag ccgacaaaag
     1681 cccggacttc agggtgttca accttacggt aagccctttc aacgatgcct ctacatcctt
     1741 ctaccacaag tctataggcg gctaccatgg agctaagctc cgccgctacc aggaccttat
     1801 tgactaccac ctttcaaagg gtaacatggc tgttttgaac atgctgaata ccaagtactt
     1861 cattcagccc ggacagaatg gtcctatagc cctacccaac cccgatgcac taggaaatgt
     1921 ttggtttgtc gattctatca aacttgttaa caaccccgac gaggaaattc aggcactcta
     1981 caactttaac cctaaaacta ccgctattgt tgataaaaag tttgaatcgc tgctagccga
     2041 ttttaaaccc gacaactccg gtgcaggaaa cattgagctg gttgaatacg caccaaatat
     2101 gctgaaatac cactcaatat ccaaccagcc aaaattggca gttttctctg aaatctacta
     2161 tcctaaaggg tggaaggcca gcattgatgg aaaacctgcg ccgcacttca gggcaaacta
     2221 tgttctccgg gcaatgatag taccggctgg ggaacaccgt atcgagttca ccttcgaccc
     2281 cccaatgtgg agggttggaa aaagtattga cctgacctcc tccctgctcc tgattttaat
     2341 attcgtaggc tggatagggt ggagcgcatt tagtaagaaa tctatctaat tgaagccatc
     2401 ctacccatga agaaccaaac caaaaaatcg gctaaggggc ggttgcgaaa ttcctacatc
     2461 tcgtctatca ttagcatttc tctggttctt ttcaccttgg gcatactggg cttgctcgtt
     2521 attagctccc aaaaactctc tgactacatg aaggagaata ttggctttac catttacctt
     2581 aacgacagcg ttagtaacgc cgatgcaaac tacttgaaaa aacttcttga tgcctcaacc
     2641 tacgtcagat cttcgcagta ctacagcaag gaagatgcag ccagccttat ggaaaaggag
     2701 ctgggagagg acttcatttc ctatcttgga tacaacccgc tttctgcctt tatcgacgtc
     2761 aggctgaagg ctgactatgc caacaacgac agcattccaa aaattgaaag ttggctgaag
     2821 cagttccagc aagtcaagga ggttgactac caaaaatcgc tggtcaacct ggtcaacgaa
     2881 aatgtaagcc ggttgggttt ggtagtgctg gcttttggcg ccctgatgct tttcatcgca
     2941 ctggtgctta ttaacaacac catccggctt tcggtttact cccgtaggtt cctcataaat
     3001 actatgaagc aggtgggggc cacctggggt tttatccgta agccattcct tctccgaagc
     3061 attctgcatg gcctctatgc ctctattatt gcaatcggcc tgctttccgg acttatttac
     3121 ggaataagaa acgaagttgc agatattctt cagattgttg aaccctacta tattatgggt
     3181 atatttgcag cggttacatt ggtgggaata gctatcaact taacagctac actcttagca
     3241 gtaaacaagt tcctgaggct aaacgccgat gacctatact actaacagct aacatttcaa
     3301 actatttaat actacgctga tatggccaaa aatgcaaaca agccggccga tagctctcag
     3361 gttgagggga agttcgcgct tggaaaggaa aattacaagt tgttgctgat aggattcggc
     3421 atcatcattc tgggatttat tctcatggcc ggtggcggtt caaaggaccc caatgttttt
     3481 gacccgcgaa tctttagctt caggcgtata acactagccc ctattgttgt gctgggcggc
     3541 tttgtgtttg aaatctatgc tattatgaaa aaaccaaaac acaagagtta gtcgccgaca
     3601 acaggaatag ccaccctatc caattttacg ctattgggta ttgtaaagat ggcaatgcta
     3661 acatctttat acgcgtagta ttgcgctgga caactccacg gtaaaggcac gcatcaagtt
     3721 tcttgccggc ttatgtccgt taaggctgct tgggtaattc atcttgcaaa actacttttt
     3781 tgtggccatt tacaccgcca acatcgcatg atgcaaaata ttaaatgctt tccccaatta
     3841 gaatagactc ttctttgctc tccttcaaat gatacagcat aaatcgttac aattattggc
     3901 caccagcatt ttcaatttat ggacgtaaat aactttaacg caaccaagga gggaacagcc
     3961 attctggttg ataagcctct tggatggacg tcatttaacg tggtaggtaa aattaggacg
     4021 ctcatccatc gggctaccgg tgatagaacc atcaaggtag gccatgctgg aacccttgat
     4081 cccttggcca ccggcctact ggttatttgc acaggtaggg caaccaagca aattgacagc
     4141 ctgatgaacg ggaaaaagga gtacttggca acgctaaaat tgggagaaac caccccttct
     4201 tttgacttgg aaacaccccc cgatgggcaa tttccgacag aacatattac tccaaccctg
     4261 attgcagaaa ccctaaagaa ctttatggga attcagcacc agactcctcc tctgttttca
     4321 gcaaagtaca taaatgggaa gagagcgtat aagtacgccc gtaagggaat tgaagtagcc
     4381 ctggaacccg tacccatcga aatatttgaa atggagatgt taagctttga gcccccattg
     4441 ctaaccctaa gaatatgctg tagcaaggga acatatatta gagcactggc ccgtgacatt
     4501 ggtgttgcgc ttggctccgg ggcgcatctt gtggccctac ggcgaactgt aagcggtgat
     4561 tttagaatat ccgaagccct gtccattcag gagctggaaa aaatattttc aagccccatg
     4621 caacaaactt aaaaatggca cgtataacgt gggactgttt gttgcataat tattttcccc
     4681 tacaataaaa gtttaagctt tagcagctgc tcaaggcatg agattcgggg aaaaacgtcc
     4741 taagaattaa ccgtccaaca atatgaagct atcacagtac aagtacaacc tgccacagga
     4801 tttgatcgcc aagtttccta ccgaaaatcg cgacgagtca cgtttaatgg tgcttaaccg
     4861 gaaaaatcat aaaattgagc accgcatttt taaggatgtg attgattaca ttgacgagaa
     4921 cgacgtggtg gtgttcaaca ataccaaggt tttcccggca cggctttatg gcaataagga
     4981 aaaaactgga gcggagattg aggtattcct cctgcgggag ttgaaccgcg agcagcggct
     5041 atgggatgta ctggttgatc ctgcccgaaa aatccgtatt ggaaataagc tgtacttcgg
     5101 tgatgatgat attttggtgg ctgaggttat tgacaacacc accagcaggg ggagaacgct
     5161 gcggttcctc ttcgacggcc cctacgaaaa gttcaaggaa actctctata agctaggcga
     5221 aacaccttta cccaagttta taagaaggga ggttgtcccc gaagacaggg aacgctatca
     5281 aactgtttac gccaagcatg agggagcagt agctgctcct actgcaggcc tgcacttcag
     5341 caagcagctg ctcaagcgta tggagataaa gggaattaat ttcgccgaaa tcacccttca
     5401 tgtagggctg ggcaacttcc gctcggttga tgtggaagac ctcaccaagc acaaaatgga
     5461 ctccgaacag atcaacattc ccgaggaagc cgccgagatt ataaacaggg caaagcaaaa
     5521 ccaccacagg gtttgcgcgg ttggaactac tgtactacgc acgcttgaaa gttccgttac
     5581 caccagcgga cagctgaaac catttagcgg atggaccaac aagttcattt tccctcccta
     5641 cgagttcagc atccccgaca tgctcatcac caacttccac ctgccacagt caaccctcat
     5701 gatgatggtc tctgcattct cgggctatga cttcctgatg gaggcctata agctggccat
     5761 taaggagaag taccgctttg gaacctatgg cgatgccatg ctcatcattt aggcggacag
     5821 taagaagcta aacgagccca acaaccaaca gccgtctgtg taaaatggac ggctgtattt
     5881 atacttcttt gctctagttg tcggccactt agacgtttaa tggtgttttg ctatgatttg
     5941 gtgcaattag ccctaaaacg ggtacccgta ctcccggagt ttttccatgt ttacccaccg
     6001 cgccagctcc ctgttgccca tcctgaaccg ctcgtctatc cagctatgga catccttgtc
     6061 aaatgtaaaa gggtgaaggg tcaaaaagga tttgtcaagc atttgtgtaa ccgataacag
     6121 aactcggtaa aggcggggta ttgaaaaaaa ataatgttta aacacggtgt tttttaaggt
     6181 aaaatgatta gaaaatctca tgaggctaac cgaaaaggtt gaaagtttaa tattttgcgg
     6241 ctcaaagttc aagtgggtgc ttgttgacag gttaaaatcc ctgctaaact gatcgataaa
     6301 tccttcgggg ttagccttaa aatcctcgaa taagcaaaca tgaacattct cactcccaaa
     6361 tagccgggca tacagctcaa ctattttgtt gtactggaac aaatcgggat tgaagagatg
     6421 gtaatcatgg aatggccttg taaactttct gtaaattccc atgtattttt taactgagta
     6481 tgtgcctccc gatttgatgt agtggccata aaccgattcg agtgcagagt gctggttgcg
     6541 aataaagatg atgatggagg cctcgccaaa ggtttccttc agccggttgg ccatcatgca
     6601 aacgtactcg cccgaacc
//
LOCUS       DAOPHX010000129         6586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_15094_length_6586_cov_5.633594, whole genome shotgun sequence.
ACCESSION   DAOPHX010000129 DAOPHX010000000
VERSION     DAOPHX010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15094_length_6586_cov_5.633594"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PK502_08995"
     CDS             complement(<1..303)
                     /locus_tag="PK502_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH00991.1"
                     /translation="MKHFYLTATLLLLFTTATFAQDTLNPDTDIIPEDTLLIQYINGN
                     LDSLLNLWYVQQTVDSNSLVANIEDDSVGLTGYPDSFYIKRLQSINSLIELPFNSIV"
     gene            complement(542..2467)
                     /locus_tag="PK502_09000"
     CDS             complement(542..2467)
                     /locus_tag="PK502_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPH00992.1"
                     /translation="MKKILLLLIFLLPMLLSAQSPKNLSTKNKRAVARYNEALAAYNR
                     YNLNEAERLLKESIKIEHNFIEAYLVLTQVYSEKGEPEKAISSAEKAVSINNNFFPKT
                     YFNLGVLNFSIGNYQKALEYFEKYLTYHETSGSMVELARNNIERCRFAIHAVANPVPF
                     NPANIGDSINTTLDEYWPSLSVDERTIVYTVRLPKEPEVGIKGTKWQEDFYTSTRNED
                     GTWSKGIPVGSPLSTVFNEGAQSLSSDGKTMYYTICQGDCDLYFSTINSDGSWGHPLK
                     LPPAINSEKSSEKQPSISPDGQMLYFVSNRPGGLGGFDIWVSVKNPNEVWGNAVNLGQ
                     PVNSPGNEQSPFIHFDNKTLYFSSNDHIGMGGQDIFVSHLTDSGKWSEPLNLGYPINT
                     CKNEEGLVVNAIGKTAYYSSNYIPDKGMDIYTFELYPEIQPIPSSYVTGTIRDYTTLK
                     PVEAHISLIDLQSKRETMNSYSSVTGGFMVCLPSDNSYALFAQAPGYLFYSENFNLKG
                     LYSFDKPYHLDVLLKPVRQGEVTIMRNVFFETDSYELLPESTVELDRVVETLSQNPTI
                     KVEIGGHTDNQGAPDYNQKLSERRAKSVVDYLLSKSVSPERVSWKGYGESQPVAPNSS
                     PEGRAMNRRTELKILEN"
     gene            complement(2639..3277)
                     /locus_tag="PK502_09005"
     CDS             complement(2639..3277)
                     /locus_tag="PK502_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HPH00993.1"
                     /translation="MGDHTTINPELESGKVLPLMEEFYTLQGEGFQTGRAAYFIRIGG
                     CDVGCSWCDSKQSWNSRLFPPVSVDEIVQRAIAHPARAVVVTGGEPTLYPLGYLCSEL
                     KKHNIQTFLETSGAYPLTGQWDWVCLSPKQQQPPVDLNYSKANELKVIIAKQADLCWA
                     EANAQRVRPDCMLFLQPEWSVHESITPLLVDYIKQNPKWRISLQAHKFMHIP"
     gene            complement(3279..5060)
                     /locus_tag="PK502_09010"
     CDS             complement(3279..5060)
                     /locus_tag="PK502_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018919111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HPH00994.1"
                     /translation="MKSITSRIILLQALVSLSLFCYAQETVTIKILETSDVHGAIFPY
                     DFIGNKPMESSMAQVYTYVKKERENSQQQVILLDNGDILQGQPTVYYSNYIDSSSCNI
                     VSRVFSYMGYDAATVGNHDIETGPLVYRKVERELAIPWMAANAINTITGKPAFKPYTI
                     IKRSGLKVAILGLITPGIPSWLPRNLWPNMRFDDMVESATYWVQQIQEKEKPDVLVGL
                     FHSGYNSSYGGANAETPLNENASLLVARKVPGFDVIFIGHDHDKLITKVENVQGDSVL
                     VLDPTSSARYISEATIQLSLDKKKRVVSKNITGTLVETRRFSPDSAFMAEFKSCYQKV
                     DSFVNQPLGEFTQTISSANAYFGPTAFMDFIHSAQLSITNADISFAAPLSFVSTINKG
                     IVRVSDMFKLYRFENFLYTMNLTGAEVDAYMEYSASLWFNTMKDANDHLLRFRQNADG
                     SMATDNRGKVELASRYYNFDSAAGIVYTVDVSKPDGNKVTILSMVNGEPFDTAKTYRV
                     AINSYRGNGGGGHLTKGCGLSAEDIQKRIVASTDKDLRFYIMKFIEQQKVITPKSFGT
                     WKIIPEEWAASGAARDHKLLFSYTGND"
     gene            5623..6381
                     /locus_tag="PK502_09015"
     CDS             5623..6381
                     /locus_tag="PK502_09015"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPH00995.1"
                     /translation="MNTYDLKGKVAVVTGAMRGIGKGIALLLARNGARVVVTDIDKAS
                     CEQVVKEIEKAGGTGVAFKLDVTNEQEIQDVVKSVHAQFGRIDILVNNAGIFIQEELD
                     GMDTAKIDKELGVNLRGTLLCTKSVLPIMKQQHYGKIVNLASIAGFVGFEVASVYSAT
                     KGAIVNLTRELALELGKYGINVNAVAPGVIETPMTDGLLEDKNVAAALLSKIPYGRTG
                     KPEDIANGVGFVVSDDSSYITGHTLVIDGGWLTM"
BASE COUNT         1652 a         1375 c         1595 g         1964 t
ORIGIN      
        1 gactatgctg ttgaatggca gctcaataag cgagttgatg gattgcagcc ttttaatgta
       61 gaatgagtcg gggtaaccgg ttaatcccac gctgtcgtct tctatgttgg ctaccaagct
      121 attgctatct actgtttgct gaacatacca aaggtttaaa aggctgtcga ggtttccgtt
      181 tatgtactgg atcagcaggg tatcttcggg tataatatcg gtgtcagggt tgagggtatc
      241 ctgtgcaaat gtagcagtag taaataatag tagcagggtt gcggttaggt aaaaatgttt
      301 catccttttt attgttagtg tttatccaac attcgcctag caaatatagt aatgattttt
      361 tgttacgttg acccgttgcg gtcaaaaaaa tcggatgtaa cccggtaata aatgttaata
      421 cccttgttaa tacctatatt tcaaattgtt tagccttgta tttctttaca tggatgaggc
      481 taaaaagggt tttaggtcat ccagccagcg gctgatggtc aaaagtgtac aggtaatgtg
      541 gttagttctc caaaattttt agctcggtgc gcctgttcat tgcccgacct tcgggagagc
      601 tgttgggtgc aacgggttgg gattctccat aacctttcca gctaaccctt tcgggcgaaa
      661 ccgatttcga cagcaggtag tccacaaccg acttagcccg ccgttccgac agcttttgat
      721 tgtaatccgg tgccccctga ttatcggtat ggccaccaat ttcaacttta atggtggggt
      781 tttgcgatag ggtttccacc actctgtcca gctcaacagt tgactcgggc aacagctcgt
      841 aggaatcggt ttcaaaaaaa acattgcgca tgatggtaac ctcgccttga cgaaccggtt
      901 ttagcagcac atccagatgg tagggtttat cgaaggagta gagtcctttc aggttaaagt
      961 tttccgagta aaacaggtat ccaggtgctt gggcaaaaag cgcatagctg ttgtccgaag
     1021 ggaggcaaac cataaaccca cctgttaccg atgaatagga gttcatggtt tcccttttgc
     1081 tctgcaaatc tataagtgaa atatgtgctt caaccggttt gagcgtggtg tagtccctaa
     1141 ttgtcccggt tacgtaggat gatggaatgg gttgaatttc ggggtagagt tcaaatgtgt
     1201 aaatgtccat tcctttatcc ggtatgtagt tggaggagta gtaggcagtt ttgccaatgg
     1261 cgtttacaac cagaccttcc tcatttttgc aagtgtttat agggtaaccc aggttgaggg
     1321 gttccgacca tttcccggaa tcggtaaggt gtgaaacgaa gatatcctga ccacccattc
     1381 ctatatggtc gtttgaggaa aagtataggg ttttattgtc aaaatgtata aagggtgact
     1441 gctcgttgcc gggtgagttt accggttgtc caaggtttac agcattgccc cacacttcat
     1501 ttgggttctt caccgatacc cagatgtcaa atccaccaag tccgcccggc cggttactta
     1561 caaagtacag catttgtccg tcgggtgaga tggatggttg tttttccgat gatttttcgg
     1621 agtttattgc gggaggaagc ttcaatgggt gtccccaact tccgtcagag tttatggtgc
     1681 tgaagtacag gtcgcagtcg ccttggcaaa tggtgtagta catggtttta ccatcagatg
     1741 agagcgattg ggctccttcg ttaaaaaccg tgcttagcgg ggagccaacc ggtattccct
     1801 tgctccaggt tccatcctcg tttcgtgtgc tggtgtaaaa gtcttcctgc catttggttc
     1861 cctttattcc tacctctggt tcctttggta agcgtacggt gtaaactatg gttcgctcgt
     1921 caaccgatag gctgggccag tactcgtcga gagtggtatt tatgctatcc cctatgttgg
     1981 ctgggttgaa gggaacaggg ttggcaacag catggattgc aaacctgcat ctttcgatat
     2041 tatttctggc aagttccacc atgctgccgg atgtttcatg gtaggttaaa tacttttcga
     2101 agtactccag ggctttttgg tagtttccaa tgctgaagtt tagaacaccc aagttgaagt
     2161 atgtcttggg gaaaaaattg ttgtttatgc ttacagcctt ttctgctgaa gaaatagctt
     2221 tctcaggttc tcccttttca gagtaaacct gcgttagcac gaggtaagct tcaataaaat
     2281 tatgctctat tttgatactc tccttcagca acctttccgc ttcgttcagg ttgtagcggt
     2341 tatatgcagc aagtgcttcg ttgtagcgtg ccaccgcccg cttattttta gtgctcagat
     2401 ttttaggtga ttgagcacta agaagcatgg gcagcagaaa tatcaggagc aggagtattt
     2461 ttttcatgct ttggagaagt ccagtagtgt gccagcagct aaattctacg ctacaatata
     2521 caaaagtaaa cgaaagtagg taaaatgtat gatttgggtt attttttatg gatactcctt
     2581 gctattaaca acctcttggt ttttgtagta gtgagttaaa gagcgaattt tattcagctt
     2641 acggaatgtg catgaactta tgcgcctgca gcgatattct ccattttggg ttttgcttta
     2701 tgtagtccac cagcaagggg gtaatgcttt cgtggacgct ccattcgggt tggagaaaaa
     2761 gcatgcagtc tggtcgaacc ctttgggcat ttgcttctgc ccagcacagg tcggcctgtt
     2821 tggcaataat caccttgagt tcgttggcct ttgaatagtt taaatccact ggtggctgct
     2881 gctgtttagg agataggcaa acccaatccc attgacctgt aagcgggtaa gcgcctgaag
     2941 tttcaaggaa tgtttggatg ttatgttttt taagctcgct gcaaaggtac ccgagagggt
     3001 aaagtgttgg ttcgccaccc gttaccacca ctgccctggc aggatgtgct attgcccttt
     3061 gaacgatctc gtccacgcta accggaggga atagtctgga gttccacgat tgcttcgaat
     3121 cgcaccacga acagcccacg tcgcatccac caattcgaat aaagtaagcc gccctccctg
     3181 tttgaaagcc ctcaccctga agggtgtaga actcctccat aaggggcaat actttacccg
     3241 actcgagttc aggatttatg gttgtatgat cacccatgct aatcgttgcc tgtgtatgaa
     3301 aatagcaact tgtggtcacg tgcagctccg ctggcagccc actcctcagg aataattttc
     3361 caagtaccga atgattttgg ggtgataact ttttgttgct caatgaactt catgatgtaa
     3421 aagcgcaggt ccttatcggt tgatgcaaca atacgtttct gaatatcctc ggccgagagt
     3481 ccgcagccct tagtcaaatg tccacctcca ccatttccac ggtatgagtt aatcgccacc
     3541 cggtaggttt tggctgtatc gaacggttct ccattgacca tagaaagtat ggtaacctta
     3601 ttgccatcgg gcttcgatac atcaactgtg taaacaattc ctgctgctga atcgaagttg
     3661 tagtagcggc tagctagctc aactttcccc ctgttatccg ttgccatgct accatcggca
     3721 ttttgcctga accgtaacag gtggtcgttg gcgtccttca tggtgttgaa ccatagtgat
     3781 gcagagtact ccatgtaggc atcaacctcg gcaccggtga gattcatggt gtatagaaaa
     3841 ttttcaaacc ggtaaagttt aaacatgtcg ctaaccctca caatcccttt gtttatggtt
     3901 gaaacaaacg ataacggcgc agcaaacgaa atatcggcat tcgtgatgct gagctgggcg
     3961 ctgtgaataa aatccatgaa agcggttggt ccaaagtatg catttgcgct ggagatggtt
     4021 tgtgtaaatt ctcccagggg ctggttaaca aaggagtcaa ccttttggta gcaactttta
     4081 aattctgcca tgaatgcgga gtcgggagaa aaccttcggg tttcaaccag tgttccggta
     4141 atattttttg aaactaccct ctttttcttg tctaatgaaa gctgtatggt tgcctccgag
     4201 atgtagcgtg ctgatgaggt tgggtcgaga accaacacgg aatcgccctg cacgttttca
     4261 actttggtta tcagcttatc atggtcgtgc ccaatgaaga tgacatcaaa tcccggtacc
     4321 tttctggcta ccagcaggga tgcattctca ttcaacggag tttcggcatt tgcacctcca
     4381 tagctggagt tatagcccga gtggaagagt ccaaccagca catcgggttt ctccttttcc
     4441 tgtatttgtt gaacccagta tgtagccgat tcaaccatat cgtcgaaacg catgttaggc
     4501 caaagatttc ggggaagcca gctgggaatg ccgggggtta ttaaccccaa aatggccact
     4561 tttaaaccgc ttcgtttaat gatggtgtag ggtttaaatg caggtttccc tgtaatggtg
     4621 tttattgcat ttgctgccat ccacggaatg gcaagttccc tttctacttt tcggtaaacc
     4681 agcgggccag tttcaatatc gtggttgcca acggttgccg catcgtagcc catgtagctg
     4741 aataccctcg aaacaatgtt acaacttgag ctgtcaatgt agttggaata gtaaacggtt
     4801 ggttgtccct gaagaatatc gccattatcc agcaggataa cctgctgttg actattttcc
     4861 ctttccttct taacgtaggt gtaaacctgt gccatgctcg attccattgg cttattcccg
     4921 ataaagtcgt atgggaaaat agctccatga acgtcactgg tttccagtat cttaatggtg
     4981 actgtttcct gagcgtagca gaatagcgac aaacttacta acgcctgtag tagaataatt
     5041 cttgatgtaa tagatttcat agtccttaac tttaaaatcg acggcgtaaa aatagcctaa
     5101 tttttccttt ttgtctggcc tgcttggctg gttaatttta agaatgagta atggttgtct
     5161 ggtctttatg tgtttattga tatggtataa tgcctgattt ttgttatgat gagttttcat
     5221 cggttttact ggtgatatcc ggcaaagctt ggtaccattc aggttattta ggtctttaac
     5281 cctaacaaaa ttttaggttt tctatcacaa gtaattttac ctataccctt taggcttcgg
     5341 ggttgggcgg agaaacccat atattggttt cacctgactc ccattacgct tggttgttta
     5401 tgggtgctta cgcttgcccc cctacacggg tgtttcatat ttttcccatc ttcttactgt
     5461 tagtagcata gtcccttatg ttgagaaaga ttaaaaaaaa gctttaaaat tttaaactaa
     5521 atgaaacgat gaattgttat ttgaataaca caaagaggct atacagaaaa aaatgtgcta
     5581 aagcaaccaa attaatgtaa accaaaataa ggaggaacaa ttatgaacac ttacgatttg
     5641 aaagggaagg tagccgttgt aaccggtgcc atgcgcggaa ttgggaaggg tatagccctg
     5701 ctgttagccc gaaatggcgc aagggttgtg gtaaccgaca ttgataaagc tagctgcgag
     5761 caggtggtaa aggaaattga gaaggcaggc ggaacgggcg tggcctttaa gctagacgtt
     5821 accaacgagc aggagattca ggatgtggtg aaatccgttc atgcccagtt tggaagaatt
     5881 gatattctgg taaataatgc cggaatattt atccaggaag agctcgatgg tatggatact
     5941 gctaaaattg acaaggaact tggcgtaaac ctacggggga cgcttctttg tacaaaaagt
     6001 gtgctgccca taatgaagca gcagcactac ggcaaaattg ttaaccttgc cagcatagcc
     6061 ggatttgttg gctttgaggt ggcaagcgtt tactcagcaa ccaagggagc catagttaac
     6121 ctaacccggg agttggcgct cgagcttgga aaatacggaa ttaacgtaaa tgctgttgcc
     6181 cctggagtta tagaaacccc tatgaccgat ggactcttgg aagataaaaa tgttgcggct
     6241 gctcttctct ccaaaattcc ttatggtcgt accggcaaac ccgaagacat tgcaaacggt
     6301 gtaggttttg tggttagcga cgacagcagc tatattaccg ggcatactct ggttattgac
     6361 ggtggttggc ttaccatgta attttttcac gaaattgttt ttgaagccga atttgtaaat
     6421 tcggcttctt ttgtatggtg tgtcgggcaa acgctactac actacactac cggcaagatg
     6481 caagttcttg cacacgccgg gttgggatga agtgctagcg aatggcaggg gtgttgcggg
     6541 tggttgcaaa tctggaagaa gccataagca agggcagcgt gctgcc
//
LOCUS       DAOPHX010000130         6582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_15103_length_6582_cov_6.216332, whole genome shotgun sequence.
ACCESSION   DAOPHX010000130 DAOPHX010000000
VERSION     DAOPHX010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15103_length_6582_cov_6.216332"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..2461)
                     /locus_tag="PK502_09020"
     CDS             complement(89..2461)
                     /locus_tag="PK502_09020"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPH00996.1"
                     /translation="MKISTISKKHSHTNRINTAGVIVLKKILPLLLLFLVGFLASSAY
                     GKNSNTIVLQLKWKHQFQFAGYYAALEKGFYEKAGLNVEIRELPSNTSAIDEVLSGRA
                     NYGVACSDLLVQYMNGKPLVLLAPIFQSSPSVLLVLASSNIYTPGDLVNKTIELSAKG
                     GGTTDILAMLGVEGIKPSQIKAVSASFSLNRLLNGQVDATEAYLTNEPYFLDKFGIPY
                     RVISPKKYGIDFYAECLFTTQEEVKRNPSRTNRFLEASIKGWEYALQNPEEIVKLIQT
                     KYKSTKTTEHLLNEAQRVKEFIQPDFINIGHSNMGRWKQMTEVLLRAGLISKTRSLNN
                     FVYEPKSTRDTISDLPWIAGIFTAVIIGLLLFHRWRIRKRINKHNLITRQQREQIEQL
                     THEVDMLRKGLETVSLRNNELEAFKDMLLANLSFEIRTPLNTIIGYSELLNGPHVKPQ
                     QTLHYTKEINKSATVLQHQIDNILDLSKLETHQLKLVYQRINLQDLFGTLHALLLNEL
                     RIFDKDHIAIQTFIDEEIDFDILSDRNLIRNIFTKLINNSVKFTPKGYIELGCKKTND
                     AMLHFWVQDSGPGISNSQLETLYEPFTQTNDSNPYGSLRLGLPVVKRLIDFLNGRIWI
                     ETTENLGTTVNFEIPYTPIGTRKRVTAKNGKHFLLQEPPQLKNKTILIVEDIHSNYFL
                     LAKMLEDTGCSIINAKRGSEAIEIFSTNHNVDMVLMDLRLADMDGIEVTRAIRQIDTK
                     VIIVAQTAYSSGVKINLSIEAGCNDFITKPVSRVDLFNILRKYLSPEGAT"
     gene            complement(2721..3908)
                     /locus_tag="PK502_09025"
     CDS             complement(2721..3908)
                     /locus_tag="PK502_09025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HPH00997.1"
                     /translation="MNSPRVTLKPGKEQSLKRFHPWIFSGAIKHVEGSPTEGEVVQVY
                     DPDDHFLAVGHFHTGSIAVRVLSFQQEEINTEFWTQRIAEALNLRKSAGLFPSGETSM
                     FRLVHGEGDNLPGLIVDIYGSTAVIQCHSTGMYLARQAIARALIEIFDGNIKAVYDKS
                     SSTLPRRENLHPSDGFIEGEGDTKGTFIENGHQFIVSFEQGQKTGFFLDQRENRHLLQ
                     SYAKGLSVLNTFAYTGGFSVYALAGGASEVTDVESSEAAVKALQQNINLNFSPTTNHK
                     VLIADVFDYLRSTSEFFDIIVLDPPAFAKHKDALKNALQAYKRLNLAAIRRLKPGGLL
                     FTFSCSQVVDKTDFRNAVFSASAISGREISILHQLSQPADHPINIYHPESEYLKGLVL
                     KVK"
     gene            complement(4037..4648)
                     /locus_tag="PK502_09030"
     CDS             complement(4037..4648)
                     /locus_tag="PK502_09030"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPH00998.1"
                     /translation="MFATSISNEEIAKLPRLVFEGETVVVSENNHLKRCLAELSNSRL
                     VGFDTESRPSFKKGRNNGISLLQLASSNLAAIIRVKSTGIPPALLTFLEDSSIIKVGA
                     AIRDDLKGLKQIHTFTPRGFIDLQQVVTNYNIENVSVRKMAAIVLGGRVSKSQQLSNW
                     EAPELTTQQVEYAATDAWVCREIYIRLLQEKPGIGERKHTAMF"
     gene            complement(4652..5266)
                     /locus_tag="PK502_09035"
     CDS             complement(4652..5266)
                     /locus_tag="PK502_09035"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HPH00999.1"
                     /translation="MDIKATVYGRNTLEFLTVAKEFCSFLEGCESYSTRNFVSASSKL
                     LPLLYYKAALLPETTPIYDEGNEQFVSENDYVAVIQKVEGLLGQHDDFAEFNYQQPEG
                     SFETITASIAEYLADIYQDLKNFTMRYQLGKEEIMNDAIWECRENFGEYWGVRLVALI
                     RAFHTLQFGDVDLDNIDEPEDSSKIDTSSWFITRRQNDSNPFQD"
     gene            complement(5470..6384)
                     /locus_tag="PK502_09040"
     CDS             complement(5470..6384)
                     /locus_tag="PK502_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HPH01000.1"
                     /translation="MMLNKMIAAMLPYLPKKLIWVFSKRYIAGETVNDAIRVARDLNH
                     KGVKVTLDILGEFIKTLEEAEKNKQEYLSLIDIIQKEKIDGNYSVKPTMFGLLIDKEV
                     CYQHLREIVAKAASYNSFIRIDMEDSSCTDLEIELYRRLKSEYPKNVGLVVQAYLKRT
                     LDDLKKLLDLNSKEIPMNYRLCKGIYVEPAEIAFKKYEEVNQHYLEDLEFMFQNGIYA
                     CIATHDKPLVEGAYKLIEKYKVPSNMLEFQMLYGVTPELRDSIVGKGYTMRVYVPFGK
                     QWFGYSTRRLKENPKMTSHIIKALFVRG"
BASE COUNT         1690 a         1410 c         1478 g         2004 t
ORIGIN      
        1 tgttcaaggg gcttcaccca acctaattga atccgtccac cttttccggg tacacgccac
       61 ccagttcaat gttaacagtt caacatggtt aagttgcccc ttcgggcgaa aggtattttc
      121 gtaaaatgtt gaaaaggtca actctactta ccggtttggt aatgaaatcg ttgcaaccgg
      181 cttcaatgct aaggtttatt ttaaccccgg agctgtaggc tgtttgcgca actattatga
      241 cttttgtatc aatttgccgg attgcacggg ttacctcaat tccatccata tcggcaaggc
      301 gtaggtccat tagcaccata tcaacattgt ggttggtgct gaaaatttca attgcctcgc
      361 ttcccctttt ggcatttatg atactgcatc ctgtatcctc aagcattttg gctagcagaa
      421 agtagttgga atggatatcc tcgactataa gtattgtttt attcttaagc tgaggcggct
      481 cctgaaggag gaagtgcttg ccgtttttgg ctgttaccct ttttcgggta ccaattgggg
      541 tgtaggggat ttcaaaattt acggttgttc ccagattctc ggtcgtttct atccaaattc
      601 taccgttcag aaaatcaata agtcgtttta ctaccggaag gccaagtctg aggcttccgt
      661 aaggattcga gtcgtttgtc tgagtaaatg gttcatagag tgtttcaagt tggctgttac
      721 ttattcctgg tccgctatcc tgcacccaga aatgaagcat ggcgtcgttt gttttcttgc
      781 aacccagctc aatatacccc ttgggtgtga acttaaccga gttgtttatc agctttgtga
      841 aaatattcct tatgaggttt ctatccgaaa ggatgtcaaa atcaatctct tcgtcaatga
      901 aggtttgaat ggcaatatga tccttatcaa atattctaag ttcgtttagc agcaatgcgt
      961 ggagggtacc aaacaaatcc tgcaggttga tgcgctggta caccagcttg agctggtggg
     1021 tttctagttt tgaaaggtct aaaatgttgt caatttgatg ctgcaggacg gtggcgctct
     1081 tgttgatttc ctttgtatag tgcaaggttt gttgtggttt aacgtgtggc ccgttcaaca
     1141 gctcgctata gccaatgatg gtgttcaggg gtgttctgat ttcgaacgat aggttagcca
     1201 aaagcatatc cttaaaggct tcaagttcat tatttcttag cgacacggtt tccaggcctt
     1261 ttcttagcat atctacctca tgagtaagct gctcaatttg ctctctttgc tggcgggtta
     1321 taaggttgtg tttgttgatt ctttttctga ttctccagcg atgaaaaagc aaaagtccga
     1381 taatgacagc tgtaaaaata ccagcaatcc agggtaggtc agatattgta tccctggtac
     1441 ttttaggctc atacacaaag ttgttcaggc tacgtgtttt gctaatcaga ccagccctaa
     1501 gcagcacttc ggtcatttgc ttccaccttc ccatgttgga gtggccaatg ttgatgaaat
     1561 ctggctgaat gaactcctta accctttgag cttcattcag caggtgctcg gttgttttgg
     1621 ttgatttata cttggtctgt atgagcttaa ctatctcctc aggattttga agggcgtact
     1681 cccatccttt aatgctggct tccaggaagc gattcgtcct cgatggattt cttttaactt
     1741 cttcctgggt ggtgaaaagg cattcggcat aaaaatctat cccatatttt ttaggtgaaa
     1801 taaccctgta gggtatgcca aacttgtcga ggaagtaggg ttcgtttgtc aggtaagcct
     1861 ccgtggcatc aacctggccg tttaaaagcc tgttgagcga gaaggaagcg ctgacggctt
     1921 ttatttgtga tggcttaatg ccctcaacac ccagcatggc aagtatatcg gttgtaccgc
     1981 caccctttgc gcttagctct atggtcttgt taaccaagtc gcccggggtg taaatattgc
     2041 tgctggcaag aaccagcaaa accgacgggc tgctttggaa aattggagcg aggagaacca
     2101 aaggcttgcc attcatgtac tggacaagca agtcggagca ggcaacaccg taatttgccc
     2161 tgcccgacaa cacctcgtca atagccgagg tgttggatgg aagttccctt atctcgacat
     2221 tcaatccggc cttctcgtaa aatccctttt caagtgctgc gtagtacccg gcaaactgga
     2281 attgatgctt ccattttagc tggagaacaa tggtgttgga gttttttccg taggctgatg
     2341 acgctaaaaa acctaccagg aatagtaata gaagtggtag tatttttttt aatacaataa
     2401 cacccgcggt attaatgcga ttggtatggg aatgcttttt agaaatggtt gaaatcttca
     2461 cgtcgggcta gctaaaagaa tcattatgat ttacactttt acgcaatgtt aaagttacac
     2521 tatttattaa aaaaaatctg catcagcttt taaaaaatta caacttacac ggcctaacgg
     2581 gtaggcgtta aattgcttca aattcaagtg tggccggcaa ttcggatgtt agaatgtggt
     2641 gctaaaatat aaaagttggg aattttctga aaaacctatt ttaatgtata atgatttgca
     2701 aatgttgtat caggttggag ctacttcact ttaagaacca gccccttgag gtattcactc
     2761 tcagggtggt aaatgtttat aggatgatct gctggttgtg agagttgatg gagaatggat
     2821 atctcacggc ccgaaatggc actggccgag aatacggcat tcctgaaatc ggttttatcc
     2881 accacctgcg agcaggaaaa agtaaatagc aaacctccag gtttcaacct tcggatggca
     2941 gccagattca agcgcttgta ggcttgcagg gcatttttga gtgcatcctt atgcttggca
     3001 aaggccggag ggtcaagaac tataatgtcg aagaattcgc ttgttgaccg caggtaatca
     3061 aaaacatcgg ctataagtac tttgtggttt gtagtggggc taaagttgag gttgatattt
     3121 tgctgcaatg cctttacagc tgcttcggaa ctttcaacat cggttacctc ggaagcccca
     3181 cctgccaagg cgtaaaccga gaagcctcct gtataggcaa aagtgttaag aacgctcaaa
     3241 cccttagcgt aactttgtag cagatgacga ttttcgcgct ggtcaagaaa aaatccggtt
     3301 ttctgcccct gctcaaacga aacgatgaat tggtggccat tctcaataaa cgttccttta
     3361 gtgtcgcctt ccccttcaat aaatccatcg gagggatgta agttttcgcg tcggggaagg
     3421 gtgctgctgc ttttatcgta aactgccttt atgtttccgt cgaaaatctc tataagagcg
     3481 cgggcaattg cctggcgggc aagatacata cctgttgaat ggcactgaat tactgcggtt
     3541 gaaccgtata tgtcaactat gagcccgggt aagttgtcgc cctcgccatg caccagccta
     3601 aacatgctgg tttctccgct tggaaaaagc ccggcagatt tccttaggtt tagggcctca
     3661 gctattcgct gagtccaaaa ttcagtattg atttcttcct gctgaaagct gagaacccta
     3721 acagcaattg atcctgtatg gaaatgccca accgccagaa agtggtcgtc cgggtcataa
     3781 acctgaacca cctcgccttc ggttggcgaa ccttctacat gtttaatggc accggaaaat
     3841 atccagggat ggaatcgttt taacgattgc tccttgcctg gtttaagcgt tactcggggt
     3901 gaattcatgg tcatttaata ggtaaaataa aaacataaat ggaaaagcta tgtaaaagat
     3961 tagggctaaa gctaaggtta tgcctggttg tatgctgtgg aattgcgtta ttcccaataa
     4021 acactcaaaa atgcgattaa aacattgctg tatgcttacg ttcgcctatg cctggttttt
     4081 cctgaagtag cctgatgtag atctccctgc aaacccacgc atcagtagcg gcatactcca
     4141 cctgctgggt ggtcagctcg ggtgcctccc agttgctcag ctgctggctc tttgaaactc
     4201 ttccgcccaa aactatagcc gccatttttc taacgctcac gttctcgatg ttataattcg
     4261 ttacaacctg ctgaagatca ataaatccac gaggtgtgaa tgtgtgaatt tgcttcaacc
     4321 cctttaaatc gtcccttatg gctgcaccta ccttgatgat tgaagaatct tcgagaaaag
     4381 tcagaagtgc aggcggaatt cccgtgcttt taactctaat aattgctgca aggtttgaag
     4441 aggctagctg gagcagagag attccattgt ttctaccttt tttaaatgaa ggtcttgact
     4501 ccgtgtcgaa tcccactaag cgactattcg aaagttcagc caggcacctc ttcaggtggt
     4561 tattttccga gaccacaaca gtttcgccct caaataccag tcggggtagt ttcgctatct
     4621 cttcgttgct gatgctggtt gcaaacatta tctaatcctg aaacgggtta ctatcgttct
     4681 gacggcgggt aataaaccag ctcgacgtgt caatttttga ggaatcttcc ggttcatcaa
     4741 tattatccaa atccacatcc ccgaattgaa gggtatggaa agcccgtatg agcgccacaa
     4801 gtctcactcc ccagtactcg ccaaaatttt cgcggcattc ccagatggca tcattcataa
     4861 tctcctcctt accgagctgg tagcgcatgg taaaattttt taaatcctgg tagatatcgg
     4921 ccagatactc ggcaatgctg gcggtaatcg tttcaaaaga accctcgggt tgctggtagt
     4981 taaattcagc aaaatcgtca tgctggccta gtaatccttc aactttttgt ataacagcaa
     5041 catagtcgtt ttcggaaacg aattgctcgt taccttcatc gtaaataggc gtagtctcgg
     5101 gcagtagggc agccttgtag taaagcagcg gcagaagctt gctgctggcc gatacgaaat
     5161 ttctggttga gtagctttcg caaccttcca gaaaggagca aaactccttg gccaccgtta
     5221 aaaactccaa cgtattacgg ccgtaaacag ttgcttttat atccatctct caccattatg
     5281 agctacaaag atagctattg tggtgaggtc ttttgtagga tagcttacaa ataaacgaat
     5341 gacagcattt ttaacagcct aacgatggct gaaactgccc aatttgtttt ggtacagtag
     5401 cacataaacc cggtagggtg gtgaaaattg tggagagttt tagtcgggaa tttctgaatg
     5461 caggtggtgc taccctctaa caaacagggc tttaataata tggctggtca tttttgggtt
     5521 ttccttcagc ctacgggtgg agtagccaaa ccattgcttc ccgaatggaa cgtaaaccct
     5581 catggtatat cccttaccca caattgagtc tcgaagttca ggagttacac cgtaaagcat
     5641 ctggaattcg agcatattgc tggggacttt gtacttttct atcagcttat atgcaccttc
     5701 aactaggggt ttgtcatggg ttgcgataca ggcgtatatt ccgttttgga acatgaactc
     5761 caaatcttcc aggtaatgct ggtttacttc ctcgtatttt ttgaatgcta tttctgcagg
     5821 ttctacgtat atgcccttgc aaaggcggta gttcatggga atttcctttg aattcaggtc
     5881 aagcaacttt ttcaaatcat ccagggttct ctttaggtag gcttgtacca ccaaaccaac
     5941 atttttggga tattctgact tgagcctccg gtatagctct atttccaggt cggtacatga
     6001 tgagtcctcc atatctattc tgatgaaact gttgtaggaa gcagccttcg caacaatttc
     6061 gcgtaaatgt tgatagcaaa cttccttatc aatcagtagt ccaaacatgg ttggctttac
     6121 ggagtagtta ccgtcaattt tttctttttg gatgatgtct atcagcgaaa ggtactcctg
     6181 cttgttcttt tccgcctctt caagagtttt gatgaattcc cccagtatgt cgagggtaac
     6241 ttttacgccc ttgtggttga gatctcgggc aacccttatg gcatcattaa cggtttcacc
     6301 ggcgatgtag cgcttggaaa aaacccaaat cagcttttta ggaaggtagg gcagcatggc
     6361 tgcaatcatt ttattcagca tcatttttta atttttatgt tgaaaacgag aatatggtta
     6421 agctatgaag tccaacttgt acatataaag ttcaaaaggt ttaaaaagtt caaagttttg
     6481 atgtttaaaa gttgatgatg aacatcttgt ttttggattt ttttaccctt cccttgtctt
     6541 cagccccagc gggggaaagg gattccccgc atagcttata aa
//
LOCUS       DAOPHX010000131         6439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_15601_length_6439_cov_4.603383, whole genome shotgun sequence.
ACCESSION   DAOPHX010000131 DAOPHX010000000
VERSION     DAOPHX010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15601_length_6439_cov_4.603383"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..214)
                     /locus_tag="PK502_09045"
     CDS             complement(38..214)
                     /locus_tag="PK502_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPH01001.1"
                     /translation="MEFSHTSHSSKGLSSAFKIGIAINVVFISVESAFGFLSNSSALI
                     ADAGHNLSDTLSTR"
     gene            complement(216..575)
                     /locus_tag="PK502_09050"
     CDS             complement(216..575)
                     /locus_tag="PK502_09050"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HPH01002.1"
                     /translation="MQNILIVINESPYGTEKAYNAMRTAMTLQKEHGDELNVKVFLMA
                     DGVFCGLPNQTTPNGYYNIERMLDAVIRQGGEVKSCGSCLSARGMEAVDLIEGVQPSN
                     MKEFTRWIVECDKVLSF"
     gene            824..1360
                     /locus_tag="PK502_09055"
     CDS             824..1360
                     /locus_tag="PK502_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01003.1"
                     /translation="MIMKMGIAQNDWCRVALATAFLAFAFFSCKDKPEHFNRSIYEQR
                     SGVAIVADTITYDVQIGVTDTSDLWSMECLKYMNQQVFIDYIFNGIYKGSMVAHDFNN
                     GMIINAEKVRQLEQQPGFSREKITKIQFKELWYIDSLGIFRKEIISYTLGLEAYSNQN
                     TFLGYSPVFVVKPTNVHP"
     gene            complement(1341..2621)
                     /locus_tag="PK502_09060"
     CDS             complement(1341..2621)
                     /locus_tag="PK502_09060"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPH01004.1"
                     /translation="MSALRVFKFGGASVKSAEGIRNMAEIIRRQTSGNLVVVVSAIGK
                     TTNALEEILSLYLAKSDEKANKLDQLKVFHTHIATELVGDSSSSHPLLAKVEQLFTEL
                     DEKMKFPPSGGYDYCYDQVVSYGELLSTTMVSFYLNQAGIRNRWIDIRKCLRSDTSYR
                     EGVVDFELSQYLCEKEFCFDSDHLFITQGFIAGTAEGTTTTLGREGSDYTAAVLANLL
                     DATDVTIWKDVDGVLSADPKIFSNTCKLDFISYQEAIELAYCGAQVIHPKTIKPIQNK
                     QIPLYVKSFINPEASGTTIGHSDVPLSLPPVLILKRNQALVSLTPHDFSFVIEESLSK
                     IFAILYRHRVKVNLVQNSAISFTICVDDEHLRLPGAIEELQKTFTVRFNSNLELLTIR
                     HYTSHVVDEMVGERIVYLQQRTRSNARFIMDARL"
     gene            2820..3833
                     /gene="fbp"
                     /locus_tag="PK502_09065"
     CDS             2820..3833
                     /gene="fbp"
                     /locus_tag="PK502_09065"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HPH01005.1"
                     /translation="MNGYNVKTLTQFIIERQADFPYATGEFTRLLYQVGVAAKLVNKK
                     VNKAGLVDVLGEAGNINVQGEEQKKLDLFANWQFIQALRNSRECCGIVSEEDQEIITW
                     DDEFSRDGNYLLCMDPLDGSSNIDVNVSIGTIFSIYRRISPRGEKPTLEDFLQPGNKQ
                     AAAGYVIYGSSTMLVYTTGRGVYGFTLDPSIGEFCLSHFSITTPTDGKIYSINEGNYD
                     HFPEGVKVYIDYCKQQDKPTQRPYSARYIGSLVADFHRNLLKGGIFLYPPTAAAPNGK
                     LRLTYECNPIAFIAEQAGGTATDGSQRILDIKPESLHQRVPFYVGSSNMVGKLQEFID
                     KHE"
     gene            complement(3974..5344)
                     /locus_tag="PK502_09070"
     CDS             complement(3974..5344)
                     /locus_tag="PK502_09070"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008021808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPH01006.1"
                     /translation="MGRIAVAGATIINEGNRFQGTVIINRGIIEEIITGASTQLTGYN
                     PEHTIDARGLLLIPGVIDEHVHFREPGLTSKGDIYSESKAAAAGGVTSIMDMPNTIPQ
                     TTTLELLDDKISTAAEKSLVNYSFYMGATNDNIEQLVKIDPLKVCGIKLFMGSSTGDM
                     LVSNREALEKIFKLSPTIIVAHCEDEASIRQNAEKVKQEKGENIPFRFHSSIRSAEAC
                     MISSMLAVELAKKHGSRLHILHLSTARELSLMNNSIPLADKKITSEACIPHLWFSERD
                     YDRLGWRIKCNPSIKLESDRQALLEGISSGLVDAVATDHAPHLAFEKDSTYFKSASGM
                     PMVQHSLQVMLELSKKGYFSVEKVVEKMCHAPATIFRIDRRGYIRKGYHADLVLINPT
                     STETVTTQSLHYRCGWSPLEGEIFHNSIETTFINGHIVYHKGKFDETIKGEALSFTQN
                     QLAYQG"
     gene            complement(5427..6179)
                     /locus_tag="PK502_09075"
     CDS             complement(5427..6179)
                     /locus_tag="PK502_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HPH01007.1"
                     /translation="MPSKEKLVIIPTYNELENIDKMLARVMELEGDFNVLIIDDASPD
                     GTAERVKAIQQNFPGRVSLIERPGKLGLGTAYICGFKWALANGYEYIFEMDADFSHNP
                     DDLVNLYKACRDGADIAIGSRYINGVNVVNWPIGRVLMSYMASKYVRLITGMRIMDAT
                     AGFVCYKGSVLKAINLDKIKLVGYGFQIEMKFTAWKLGYKIVEVPIIFTDRKFGVSKM
                     SGGIFNEALWGVIAMKLKSIFKPYQKLNLPER"
BASE COUNT         1760 a         1406 c         1323 g         1950 t
ORIGIN      
        1 aattagcgcg ctgcggttgc agaggcaaga tgcacaatca acgggtggaa agggtgtcgc
       61 ttaggttatg cccggcatca gctataagcg ccgatgaatt ggaaagaaaa ccaaatgcag
      121 attctacgga aataaaaacc acatttatgg caatgccaat tttaaaggcg ctgctgagac
      181 ccttgctgct atggctggta tggctaaact ccattttaaa atgataaaac cttatcacac
      241 tcaacaatcc accgggtaaa ctctttcatg ttgcttggtt gcactccttc aataagatca
      301 acagcctcca taccccttgc cgacaggcag ctaccgcaac tcttaacttc tcctccttgc
      361 ctaatcacgg catcaagcat acgttcaata ttgtagtagc cattgggagt ggtctggttt
      421 ggcagtccgc aaaaaacacc atcggccatt aaaaaaacct tcacgtttag ctcatcccca
      481 tgctcctttt gcagggtcat ggcagttctc atggcattat acgccttttc tgttccgtat
      541 ggcgactcgt taattacaat caggatgttc tgcatcgttt tatttttgtt ggtaacacat
      601 tatactattg tttgttcgtt ataacactat gtgcttttat gctgcataaa agtagtgata
      661 tgttgaaaat gtgtaatctt tggctatgaa catttttaag ggtatttttt tgaaaattaa
      721 gtacccttta caatcgctgc tggttggtta atcccggctg attattttta ttgggcaaat
      781 tgcaataatt ttgcttttgt ggtttacgtt atatgcttcg tttatgatta tgaagatggg
      841 gattgcgcaa aatgattggt gtagggttgc tttagcgact gcttttcttg cttttgcctt
      901 tttttcgtgc aaagacaaac ctgaacattt caataggagc atctatgagc aaaggtctgg
      961 agtagctatt gttgccgaca ctattaccta tgatgttcag ataggagtta ccgatacctc
     1021 cgacctatgg tcaatggaat gcctgaagta catgaatcag caggttttta ttgattatat
     1081 ttttaatggc atctacaagg gcagcatggt tgctcatgat tttaataatg gtatgattat
     1141 taacgctgaa aaggtaagac agctggagca acaacccgga ttttcaaggg agaaaatcac
     1201 caaaattcag tttaaggagc tttggtacat cgactctctg ggcattttca gaaaagaaat
     1261 tattagctac acactggggc tagaagcgta cagcaaccag aatacgtttt tgggctatag
     1321 ccctgtattt gtagttaaac ctacaaacgt gcatccataa taaacctggc attacttcgc
     1381 gtacgctgct ggaggtaaac tatcctttcg ccaaccattt catctactac atggctggta
     1441 tagtgcctaa tggtgagcag ttcaaggttg ctgttgaatc gaaccgtgaa ggttttttga
     1501 agttcttcta tggctcctgg taggcgtaaa tgctcatcgt caacacaaat ggtgaagctg
     1561 atagccgaat tttgaactaa gttcacctta acccggtgcc ggtaaagaat ggcgaatatt
     1621 ttgctgaggc tttcctcaat gacaaaggaa aagtcgtgtg gagtgagcga taccaatgct
     1681 tgattgcgct ttagaattag cacggggggt aagctcaatg ggacatcaga gtggccaatg
     1741 gttgtacccg aagcttcggg gttgataaac gattttacat acaagggaat ttgcttattc
     1801 tgaatgggtt ttatggtttt gggatgaatt acctgggctc cgcagtaggc cagctcaatg
     1861 gcctcctggt aggatataaa atcaagttta caggtgttgg aaaagatttt tggatcagcg
     1921 ctcagcactc cgtcaacatc cttccatata gttacatcgg ttgcatcgag caggttggct
     1981 agcacggcag ccgtgtaatc cgatccctct cgtcctagcg ttgtagttgt gccttcggca
     2041 gttccggcaa taaaaccctg ggtgataaat aaatggtcgg agtcaaagca aaactctttt
     2101 tcgcagagat actgtgatag ctcgaaatca accaccccct cccggtacga tgtatcagaa
     2161 cgtaagcact tccgaatgtc aatccaccta tttcttatgc cagcttggtt taggtaaaag
     2221 ctaaccatgg tggttgacag aagctctcca tacgatacaa cctggtcata gcagtagtca
     2281 taacccccac ttggaggaaa tttcatcttc tcatcaagct cggtgaatag ctgttcaacc
     2341 ttggctaata gaggatgtga agagctgctg tcgcctacca gctccgtggc tatgtgggta
     2401 tgaaaaactt ttagctgatc caacttatta gctttttcgt cgctttttgc taaatacagg
     2461 gaaagaatct cctctaatgc gttggttgtt ttccctatgg ccgaaaccac cactacgaga
     2521 tttcccgatg tttgccgacg gataatttcg gccatattcc ttatcccttc tgccgatttc
     2581 accgaggcgc ctccaaattt gaaaacacgc aatgccgaca tattctttaa aaatttattc
     2641 aaacatacat gtttgattcg atttgggcaa cttatcgata taatcccggc tttcaagtgt
     2701 aacgttcttg tatatcttcc gcaaacgttt taactttggc aattgtgtta cttaacatgt
     2761 ttcgatttgg gtaaattggt tatctttatc aacgttaatc agtagcattt ttctgcccca
     2821 tgaatggata taatgtaaaa acgctcacgc agttcatcat tgagcggcaa gcggatttcc
     2881 cctatgcaac tggcgaattc accaggctgc tttaccaggt aggtgtagct gcaaagcttg
     2941 taaacaaaaa ggttaataag gctgggctcg tggatgtgct gggcgaagct ggcaacatca
     3001 acgtgcaggg agaggagcag aagaaactcg acctgtttgc caactggcag ttcatacaag
     3061 ctctcagaaa cagccgagag tgttgtggaa ttgtttcgga ggaggaccag gagattataa
     3121 catgggacga tgagttctca cgcgatggca actacctgct ctgtatggat cctcttgatg
     3181 gctcatccaa tattgatgtt aacgtttcca ttggaacaat attcagcatt tatcgacgta
     3241 tcagcccccg gggagagaaa cctaccctag aagatttcct tcaacctggc aacaaacagg
     3301 ctgcagctgg ttacgtgatt tacggttctt caaccatgct ggtttataca accggccgcg
     3361 gagtttacgg ttttactctt gacccctcca ttggcgaatt ttgcctttca cactttagta
     3421 ttactacacc taccgatgga aaaatatact ccatcaacga gggaaactac gatcattttc
     3481 ctgaaggagt gaaagtttac attgattact gcaagcaaca ggataagcct acccagcgac
     3541 catactcagc ccgctacatt ggctcgctcg tggccgattt ccatcggaac ctgttaaagg
     3601 gaggaatatt cctttatccg cccaccgccg cagcacctaa tggtaagctg cggttaacct
     3661 atgagtgtaa ccctattgca ttcattgccg agcaagcggg gggaacggca accgatggta
     3721 gccagcgaat cctcgatata aagcccgaat cgcttcacca acgggttcct ttttacgtgg
     3781 gctcgtcaaa tatggtgggc aaacttcagg agtttattga taaacacgag taacaattac
     3841 ctccagctaa aattaaactg cttacgccaa atattttacc ggaataaggc ttgagcgtgt
     3901 tgaccctact ggtgatgggt tgaacttgta gctctatatt acgttggagg ttttcaatgc
     3961 tgttggcctt tcatcaaccc tgataagcca gttggttttg ggtgaagctt aaggcttcgc
     4021 ctttaatggt ttcgtcaaat tttcctttat gatagacaat atgaccgttt ataaaggttg
     4081 tctcaatgct gttatggaaa atttcaccct ctaacggtga ccaaccacac ctgtagtgaa
     4141 gtgattgggt agttactgtt tcggtagatg ttggattaat cagcaccaaa tcggcatggt
     4201 agccctttcg gatatagcct ctgcggtcaa tcctgaaaat ggtggctgga gcatggcaca
     4261 tcttctcaac caccttttct actgaaaagt accccttttt cgaaagttcc agcataactt
     4321 gtagtgaatg ctgaaccatt ggcattcctg aggctgactt aaagtaggta ctatcctttt
     4381 cgaaggctaa atgtggagca tgatctgttg ccacggcatc aaccaaacca ctacttatgc
     4441 cttccagaag agcctgcctg tcggattcca gcttaatact tggattgcat tttatcctcc
     4501 atcccaacct gtcatagtcc ctttcgctaa accataggtg agggatacat gcttctgagg
     4561 taattttttt atcagcaaga ggaatactat tattcataag cgaaagctcc ctggctgttg
     4621 aaagatgaag aatatgaagt cttgaaccat gcttcttggc caactctact gctagcattg
     4681 aagaaatcat acaggcttcg gcactgcgaa tactggagtg aaatctaaaa ggaatgtttt
     4741 ctcctttttc ttgcttgact ttttccgcat tttgacgtat gctggcctca tcttcgcaat
     4801 gagcaacaat gattgtaggc gacagcttaa aaatcttttc gagtgcttcc cggttgctca
     4861 ccaacatgtc tccggttgac gagcccataa atagcttgat gccacatact ttcaatggat
     4921 caatttttac cagctgctca atgttatcat ttgttgcccc catgtaaaat gaatagttaa
     4981 ccagcgattt ttcagcagct gtactaattt tatcatcaag aagctcaaga gtggtggttt
     5041 ggggaatagt gttgggcatg tccattatag aggtaacccc acctgctgct gctgctttcg
     5101 attcgctgta tatatcccct ttactggtta atcccggctc cctaaaatga acatgctcgt
     5161 ctataacccc tggaattagt agcaatcccc tggcatctat tgtatgttct ggattataac
     5221 ccgttagttg agttgaggct ccagtaatta tttcttctat tattcctcta tttattatta
     5281 ctgtcccttg aaacctattc ccctcgttga taatcgtggc accagctact gcaattcgtc
     5341 ccatcataat tcttttaacg cttttatgca acaccactaa tattccaaaa actctaatgc
     5401 tagtagtatt ttaaatatag aagttgctat cgctcaggta gattcaactt ttggtagggc
     5461 ttgaatatac tttttagctt catggcaatt actccccaaa gagcctcgtt gaaaatgcct
     5521 ccgctcattt ttgaaactcc aaattttcta tcagtaaata ttataggtac ctccacaatt
     5581 ttatacccca gcttccatgc ggtaaacttc atttcaatct gaaagccata cccaacaagc
     5641 ttaattttat caaggtttat agcctttaaa acgcttccct tatagcaaac aaagcctgct
     5701 gtagcatcca taattctcat tccggttatc aaccttacat atttcgatgc catatacgac
     5761 atgagtactc tgccaattgg ccaattaacc acatttaccc catttatgta tcttgaacca
     5821 attgctatgt cggcaccatc cctgcaggcc ttataaagat tgaccaaatc gtcgggatta
     5881 tgtgaaaaat ccgcatccat ttcaaatata tactcgtaac cattggcaag cgcccacttg
     5941 aaaccacaaa tgtaagcagt ccctaatcct aatttccccg gcctctctat caatgaaacc
     6001 ctacctggga aattctgctg aatggctttt accctttctg ctgtcccatc gggtgaagca
     6061 tcatcaataa ttaaaacatt aaaatccccc tcaagctcca tcaccctggc tagcatctta
     6121 tctatattct caagttcatt ataggtcgga ataattacta acttttcttt actgggcatt
     6181 gttatgtatt attaagttag ggttggcata tgtaatgtga atatgcgaat ataatgcttt
     6241 tatgcatttg gtttgagaga atccatctgg ttttcaaaat cagacaaaat taaattatat
     6301 gagaaccaaa taattaaaca tccaaaataa attttatttt aaaaagatgt aaaaaaggtg
     6361 taaaaaagtt tggatggatt taggagaggt ggtatatttg cagccgcaaa aggggggggt
     6421 tagagccccc ggccgagta
//
LOCUS       DAOPHX010000132         6378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_15836_length_6378_cov_4.907639, whole genome shotgun sequence.
ACCESSION   DAOPHX010000132 DAOPHX010000000
VERSION     DAOPHX010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15836_length_6378_cov_4.907639"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1891)
                     /locus_tag="PK502_09080"
     CDS             complement(<1..1891)
                     /locus_tag="PK502_09080"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPH01008.1"
                     /translation="MKKFLLASVVTLGSSWALLAQPERMPFGENMQYSSGKHLFNAES
                     SGAKGINEMYFSDDFEGSSYILSDKWEILRSSTLDYSSTISAANPMWFHCTPESFNGN
                     GATYIKFGRGSAAISYTAPDFTWLVCKDTLPIPQNGEAYLKFWLWYSSNFDQGYSTHF
                     YILAYNTDDHAIDTLNYWGEYDSTSISNQYARRITVPLSLLAGKNIRLAFVYENRNNE
                     NKGIQLAVDEVQITNSNSPDISLKAVPFTYSQIPYFTFDSLFFNLYADIYNTGAAISD
                     TIEIVATSEQLPDINAFATITDTLNEGEQRLAKLNGQAKITENTNYTIGITSSTDQDS
                     ITANNSDNIVFSVSQSVYATDHGATGGFTLGENQPFGNIFKVNKPSFIKGIQVGWSPI
                     STIPEAEYPIPFWVAVYQLDPLDSSCYFPVKTGYFEKQYSQVGASSDYPFDEPIYCLP
                     GFSYFVTVTQLSSASLGVGCDGNSYGSFWKRNGSTFKPEKLANPTIGNAAIRLYFNKP
                     IENPIVTFTVKNENNEPVQNARVYVREVDSTLITGVSGEASIELSNGTYTCRLDSAGF
                     APVTKQVKVFYQDLFIDFKLQKTYQAKFKVLDEDLLTPIAGAEINILSESIDITFPEE
                     PYLTDQ"
     gene            complement(2178..4031)
                     /locus_tag="PK502_09085"
     CDS             complement(2178..4031)
                     /locus_tag="PK502_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPH01009.1"
                     /translation="MKEHPFVIPFTIGLTFVILYTVVMWTIWVVRLTRREKKIIWKRL
                     LTVKTLRGIWETIREGLFHRNIFKKNVILGFMHMSLAFGWFMLIVLGNLEARLYPHGL
                     INPPYYPIFFEFFRHDLSPLREYEEIFTFLMDFFLLLVISGIGIAFFKRYKSKVMGMH
                     RTTKHILIDRIALTSLWLIFPFRILAESVTAGLYNTGHFFTNFLGQQVIYGLPLDQLY
                     YPSWWAYSIALGSFFVALPFSRYMHIPTEILLIFLRKYGVREKDSHSSYTNVELNACS
                     RCGICIDSCQLSTDLGISDTQPTYFIRDLRYSKPSERLRETCMLCMRCLKACPVGIEI
                     TQIRKNERSEHQLFSPTSYSYLNGYAYPKAKVAFFAGCMGHLTPNIIKSTLLLLDAAG
                     IDYTFIDRNGSICCGRPLQLNGEVDAAKQLIDKNNRLIYETGANCLVTTCPICASEFK
                     KNYHMSIPIYHHSQYLWELTNQGRLVFNQSNLNVAYHDPCELSRGLGVVDEPRKLLKS
                     AVVLTENSNQTKQNSLCCGGSLAITNTSAFQKSTIAASTVLQLANNTTDLIATACPLC
                     KKTLAAAQNDVPVQDISEIMVKALMDSRKASLSEAKVKMASPKLEVVEEYK"
     gene            complement(4028..4363)
                     /locus_tag="PK502_09090"
     CDS             complement(4028..4363)
                     /locus_tag="PK502_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPH01010.1"
                     /translation="MIDFGYAPLKINIHDLDKADLSLMDWITARDESFRLCIACGSCT
                     ATCTAGNFTNFSFRELCHSIRRGEVKKAVRESEKCMLCGKCTLVCPRNVNTRNIIKLV
                     RKANQNFRV"
     gene            complement(4360..5382)
                     /locus_tag="PK502_09095"
     CDS             complement(4360..5382)
                     /locus_tag="PK502_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPH01011.1"
                     /translation="MLKKVAVIGGGVAGLEAARQLAALGYQTTVYEKNDHAGGHTSEW
                     DRLFPNRRPAVEVVDTLKSQLNGNPAIVLSTPITELLPNGDKFTLKTNNKSFEADAVL
                     IATGFELFQAERKEEYGYGIYDNVITSAELEKMFTEHGAPLMRNGEKPKRVAFVHCVG
                     SRDEKVNHLYCSKVCCVTAVKQAIEAKEAIPEAEIFCLYMDLRMFGPGYEELYKEAQQ
                     KGINFIRGRLSEASELDGGRLLIKTEDTLASRPLKMSVDMLVLMVGMTPAEGTSKLIY
                     NLGLKPNADGFIGVSDYHLKPSETGIRGVYTAGACSGPNNITDSINSARSAVVEIHRY
                     LSVKEP"
     gene            complement(5532..>6378)
                     /locus_tag="PK502_09100"
     CDS             complement(5532..>6378)
                     /locus_tag="PK502_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HPH01012.1"
                     /translation="IQTVVARNFSLMRENGYQNYNPSCVTSFGIYTEILETWHHFPEE
                     KEKTREYLKKATGREFEEPANLVHTSDVLYKFRNEIAAKAKYRLVNRETGKPLKVVDH
                     IGCHYAKMFPSKGIGGAEYPQVLAGLVEAFGGEVVDYPERRHCCGFGFRQYFVQANRG
                     YSLAASKKKFESMEPFKPDFIITNCPGCNYFMDRWQYVLAEAEGKTYGQDGYGIPVLS
                     YEEVAGVVLGFDPWEIGLQMHQTDVEPLLKKIGVEYDPDKKFKGVNNETLSLPDKPNN
                     IKFIV"
BASE COUNT         1659 a         1363 c         1497 g         1859 t
ORIGIN      
        1 tttggtcggt taggtagggc tcttcaggga aggtaatatc aattgattca gacagtatgt
       61 tgatttctgc tcctgcaatt ggagttagca ggtcttcgtc cagcaccttg aatttagcct
      121 ggtaggtttt ttggagttta aaatcaatga ataggtcctg gtaaaagacc tttacctgct
      181 tggtaactgg cgcgaagcca gcggagtcga gcctgcaggt gtaggtaccg ttggaaagtt
      241 caattgatgc ttcgcctgaa acccccgtta taagggttga atcaacctca cgaacataca
      301 ccctggcgtt ttgcacgggc tcattgtttt cattctttac tgtgaaagta acaatgggat
      361 tttcaatagg cttgttgaag taaagcctga tggctgcatt gccaatggtt gggtttgcca
      421 gtttttcagg tttaaacgtg cttccatttc ttttccaaaa cgagccgtag ctgttgccat
      481 cgcaccccac accaagacta gcgctactta actgggttac agtaacaaag taggaaaatc
      541 ctggtaagca gtagattggt tcgtcaaagg ggtagtcgct tgaagcgccc acctgggagt
      601 actgtttttc gaaatatcct gtttttacag ggaaataaca ggaactatcc agggggtcaa
      661 gctggtaaac tgctacccag aatggaatgg ggtattctgc ttcgggaata gtgctgatag
      721 gcgaccagcc cacctgaata cccttgataa atgacggctt gttaaccttg aaaatgttgc
      781 caaagggttg attctctccg agtgtaaaac cacctgttgc accatggtct gtagcataaa
      841 ccgactggga tactgaaaaa acgatattgt ccgagttgtt ggcggttatg ctatcctgat
      901 ctgttgagga ggttatacct atggtgtagt ttgtattttc agtaatttta gcctgtccgt
      961 tgagcttagc taaacgttgc tcaccttcgt ttaaggtatc ggtgatagtg gcaaaagcat
     1021 taatatcagg aagttgctcc gatgtggcaa caatttcaat ggtgtcgcta attgctgctc
     1081 cggtattgta gatgtcagca tagaggttga aaaaaagtga atcgaaggta aagtagggta
     1141 tctgggagta ggtgaatggg actgccttca aagaaatatc cggactgtta ctattggtaa
     1201 tttgaacctc gtcaacagct agctgaatgc ctttattttc gttattccta ttctcataaa
     1261 cgaatgcaag cctgatattt ttgcctgcaa gcagtgaaag tggtactgtt attcggcgtg
     1321 catattgatt tgaaatgctg gttgaatcat actcacccca atagttaaga gtgtcaatgg
     1381 cgtgatcatc ggtattgtat gcaagtatgt aaaaatgggt ggagtaaccc tggtcaaaat
     1441 tgctggagta ccagagccaa aattttaggt aagcctcacc gttttggggg ataggaagag
     1501 tatccttaca aacaagccag gtaaaatcgg gagcagtgta ggaaatagca gcagaacctc
     1561 ttccaaattt aatgtaggtg gcgccattgc cgttgaagct ttccggggtg cagtggaacc
     1621 acattggatt agcagctgat atggtgctgc tataatccag agtgctgcta cgcagaattt
     1681 cccatttatc cgacaaaatg tatgaggaac cctcgaaatc gtcggagaag tacatttcat
     1741 taattccctt tgctccgcta ctctcagcat taaataaatg tttccctgag gaatactgca
     1801 tattttcgcc aaaaggcatt cgctcgggtt gggccagcaa tgcccatgag ctgccaagtg
     1861 taacaacgct agctagtaga aacttcttca taggatagta tttgttagaa catgattgaa
     1921 accaatatag taatagtata tgtgtaaaaa taggaaaatc cggcaaataa aattagaact
     1981 tagaataaaa aatgaattcc ttctggattt attacactta tggtctagtc ctttaacgaa
     2041 gaaggatgtt gaaattgatt acatgtctta aaaaaaattt gattaaggtt gtgtaatcct
     2101 aatcattagt tgctggaaag taataaatac cggcttgaaa gttttgaaag gcagaatgcc
     2161 atgagtactt accgcaccta cttgtattcc tcgactacct caagtttggg gctagccatc
     2221 ttaactttag cctctgaaag gcttgcctta cggctgtcca taagcgcttt caccataatt
     2281 tcggatatat cctggacggg gacatcattt tgggcggcag caagggtttt cttgcaaagc
     2341 gggcatgctg tggctatcag gtcggtagtg ttattggcta gttgcaaaac ggttgatgcc
     2401 gcaattgttg acttttgaaa tgcagaggtg ttggtaattg caaggcttcc cccgcagcat
     2461 agggaatttt gtttggtttg gttgctgttt tcggttagaa ccacagcgct ctttaggagc
     2521 ttgcgtggct cgtcaacaac accaagacca cggcttagct cacacggatc gtggtaagca
     2581 acattcaggt tggattggtt aaaaaccagc ctgccttggt tggtaagctc ccagaggtac
     2641 tggctatggt ggtatatggg aatgctcatg tggtagttct tcttaaactc gctggcgcaa
     2701 atggggcagg tggttaccaa gcagttggcg cctgtttcgt aaataagccg gttattttta
     2761 tcaataagct gctttgccgc atcaacctct ccgttaagct gcaacggcct accacagcaa
     2821 atgcttccgt ttcggtcgat aaaggtgtaa tcgatcccgg cagcgtcgag aaggagaagc
     2881 gttgatttaa ttatgttggg ggttaaatgc cccatgcacc ctgcaaaaaa tgcaacctta
     2941 gctttggggt aggcgtaccc gttcaggtag ctatagctgg ttgggctaaa aagctggtgc
     3001 tcgcttcgtt cgttctttct tatttgtgtg atttcaattc ctaccgggca tgctttaaga
     3061 catctcatac aaagcataca ggtttctcta agcctctcgc ttggcttgct gtatctaaga
     3121 tccctaatga agtaggttgg ttgggtgtca gaaattccca aatcggtgct tagctggcag
     3181 gagtctatgc agataccgca cctggagcaa gcgtttagct caacgttggt gtaggatgag
     3241 tggctatcct tttcccttac accatattta cgaagaaaaa tcagcaaaat ttctgttgga
     3301 atgtgcatgt acctagaaaa gggtagggca acgaagaacg accccaacgc aatggagtat
     3361 gcccaccatg aggggtagta tagctggtcg agcggaagac cgtagataac ctgttggccg
     3421 aggaaatttg tgaagaaatg gccggtgtta tacaatccgg cagtaacgct ttcagccagt
     3481 attctgaaag ggaaaatcag ccaaagggag gtaagggcta ttcggtcgat gagaatgtgt
     3541 ttggtagttc ggtgcatccc catcacctta cttttatacc ttttaaaaaa ggcaatgcca
     3601 attccggaaa tgaccagaag caggaaaaag tccattagaa aggtgaaaat ctcctcatat
     3661 tccctcagcg gggaaaggtc gtggcggaaa aactcaaaaa aaatagggta gtagggaggg
     3721 ttgattagcc cgtgggggta aagtcgggct tccaagttgc ccagcacaat aagcatgaac
     3781 catccaaagg cgaggctcat gtgcataaag ccaagaataa catttttctt aaagatattt
     3841 cgatgaaaca aaccctctct tatggtctcc catattcctc ttagcgtttt aactgttagc
     3901 agtcttttcc atataatttt tttctcacgc cgggtaaggc gtactaccca aatggtccac
     3961 atgacaaccg tatagaggat aacaaaagtt agccctatgg tgaacgggat tacaaatgga
     4021 tgttccttca taccctgaaa ttttggttgg cttttcgaac cagctttatt atgttacgag
     4081 tatttacgtt gcgaggacaa accaaggtgc actttccgca aagcatgcac ttttccgatt
     4141 ccctcacagc ctttttaact tctcctctac gaatgctgtg gcaaagttcc ctaaagctga
     4201 agttggtaaa atttccggca gtgcaggttg ccgtgcagct tccgcaggca atgcagaggc
     4261 ggaacgattc gtctcgagct gtaatccagt ccattaggga aagatcagcc ttgtccaaat
     4321 cgtgaatgtt tattttaagg ggtgcgtagc caaaatcaat catggttcct tcacgcttaa
     4381 atacctatga atttcaacta ctgccgaacg ggcagagttt atcgagtcgg tgatgttgtt
     4441 aggtcccgaa catgcaccgg cagtatatac acctctaatg ccggtttcgg aaggtttaag
     4501 gtgatagtcg cttaccccaa taaacccatc ggcatttggt ttaagcccaa ggttatatat
     4561 aagtttggaa gtgccttcgg ctggtgtcat gcccaccatc aaaacgagca tatctaccga
     4621 cattttaagg ggacgggaag ccagggtgtc ctcagtctta ataagcaatc ttccaccatc
     4681 cagctcgctt gcctccgata gcctgccccg gatgaagttt attccctttt gctgtgcttc
     4741 cttgtaaagc tcctcatagc caggcccaaa cattcggagg tccatgtaga gacagaatat
     4801 ttcagcctct gggatagcct cttttgcttc gattgcctgc ttaactgctg ttacgcagca
     4861 aacttttgaa cagtaaagat ggttcacctt ttcatcgcgg ctacccacgc agtggacgaa
     4921 tgcaactcgt ttgggttttt cgccatttct catcaggggc gctccatgtt cggtgaacat
     4981 cttttctagc tcggccgagg taattacgtt gtcatatatt ccgtaaccat actcttcttt
     5041 gcgttcggct tgaaaaagtt caaaacccgt ggcaatcagc acagcatcgg cttcgaaaga
     5101 tttattattg gttttaaggg taaatttatc cccgttgggt agcagttcgg ttatgggtgt
     5161 agaaaggacg atggccggat ttccgttaag ctgggatttg agcgtgtcaa ccacctcaac
     5221 agcgggtctt ctgtttggga ataacctgtc ccactctgag gtatgaccgc cggcgtgatc
     5281 atttttctca tatacggttg tttggtaacc caacgctgct agttgcctgg cagcctccag
     5341 cccagcaaca cctccaccta tgacagctac tttttttaac atcgttaact agtttgacta
     5401 cattactttg cctatcatta cagcattact ggttttacta tttgtttcct aagagatggt
     5461 tacaattcca ctggtttttt gctgctgacg tatgatttgc aatacagctg tgcatccctt
     5521 ctgcattagt tttaaacgat aaatttaata ttatttggtt tatcgggcaa gctaagggtt
     5581 tcattattta cacctttaaa cttcttgtcg gggtcgtatt caaccccaat ttttttaagt
     5641 aggggctcta cgtcggtttg gtgcatctgc aatcctatct cccatggatc gaatcccaga
     5701 actactccgg caacctcctc gtatgagaga acgggtatcc cgtaaccgtc ttgtccatag
     5761 gttttccctt cagcttcggc cagaacatac tgccatctgt ccataaaata gttacaaccc
     5821 ggacaattag taatgatgaa atcgggcttg aagggttcca tggattcaaa tttctttttt
     5881 gaagcagcca gggagtatcc cctgttggcc tgaacaaagt actggcggaa gccaaaccca
     5941 cagcagtgcc tacgctcagg atagtcaact acttcgcctc caaaagcttc taccagacct
     6001 gccagcacct gggggtattc ggcaccgcca atccctttgc tgggaaacat tttggcataa
     6061 tggcagccaa tgtggtcaac taccttcagc ggttttccgg tttccctatt caccagtctg
     6121 tatttagctt ttgcagctat ttcgttacga aacttataga gcacatcgct agtatggacc
     6181 aggttggcgg gttcttcaaa ttctcttccg gttgcctttt taaggtattc acgagtcttt
     6241 tccttttcct cggggaaatg gtgccatgtt tcaagtattt cggtgtatat accaaatgag
     6301 gttacacagg atgggttgta gttttggtaa ccattctcac gcatcaggga aaagttgcgc
     6361 gccacaactg tttggatg
//
LOCUS       DAOPHX010000133         6353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_15917_length_6353_cov_6.203239, whole genome shotgun sequence.
ACCESSION   DAOPHX010000133 DAOPHX010000000
VERSION     DAOPHX010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15917_length_6353_cov_6.203239"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1564)
                     /gene="yidC"
                     /locus_tag="PK502_09105"
     CDS             complement(<1..1564)
                     /gene="yidC"
                     /locus_tag="PK502_09105"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPH01013.1"
                     /translation="MDRNTILGLVLIFLIMIGFGYLAAPSKEEREAYVRKADSLARVQ
                     AEYEQKRAEEEKAKAAMLADTIQKKEVQSQVGSFAPALEGERKFISLENDLMRVVVSN
                     RGGRIYSVELKKFKTHAGEPLVLFNGDDNEFGLQFWGENNAVKTNDLFFVPQTSDTAV
                     TASESQSASLTMRLQIADSSYIDYIYTVKPNSYMVDFDIRFNGMTKSIGGQAGSIDLL
                     WNLKLNRLEKGIENERNYTTIAYTFLNNDYEELNPRSQDEKKELTTKLKWVDFKHQFF
                     SSILVANEHFINSDLKMTNLNDSLHVKAMDARLSLPFKNQNDETLKLGFYFGPNHYKT
                     LKEYNQGFEKVVPLGRNIVRWINKYVIILLFYWLSSFLTNYGIIVLIMTIIIKLVLLP
                     LTYKSYLSSAKMRVLKPQIDEISKKYPKKEDAMKKQQAVMALYKKVGVSPMGGCLPVL
                     IQFPILIAMFRFFPASFELRQQSFLWAEDLSSYDSILHLPFTIPWFGDHISLFALLMA
                     ISMVITSKMSNDQ"
     gene            complement(1643..3256)
                     /locus_tag="PK502_09110"
     CDS             complement(1643..3256)
                     /locus_tag="PK502_09110"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPH01014.1"
                     /translation="MPHNTKYVFVTGGVASSLGKGIISASLGKLLQSRGFSVTIQKLD
                     PYINVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLNLPTSQMNNVTTGKIYQTVI
                     SKERHGDYLGKTVQVIPHITDEIKRRIKLLGTKHKLDVVITEIGGTVGDIESLPYIEA
                     VRQLRWELGPTNSAFIHLTLVPYLSASGELKTKPTQHSVKVLLENGIQPDVLVLRTEK
                     PLNDDIRRKVALFCNVDSSAVVESIDVKTIYEVPLLMHKEKLDETIMKKLNLPVQEPP
                     KLDDWNEFINKLKNPKSKVKVGLVGKYVELHDAYKSIVESFIHAGAVNECHVELEMIQ
                     SESITASNVKEKLGHLQGILVAPGFGHRGVEGKIEAIRYARENQVPFFGICLGMQCAV
                     VEFARNVLGLQEANSTEMVRNTPFPVIDLMEEQKGITDMGGTMRLGSYPCQVKKGTKA
                     YMAYHKLETHERHRHRYEFNNQFLPQFEAAGMVAAGINPETNLVEIMEISSHPWFVGV
                     QFHPEYKSTVINPHPLFVEFVKATMNFGG"
     gene            complement(3463..5544)
                     /locus_tag="PK502_09115"
     CDS             complement(3463..5544)
                     /locus_tag="PK502_09115"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HPH01015.1"
                     /translation="MSLENNNEITMSTNDLTDPKDQNLANSEGTMDSSSLAEQENEPV
                     ALERIEISNQLGDDPQGIHFDASTTVKSEGESAEATNIPVNEIELPAESEELEEVEDI
                     EDGDEEVDEESEEIKIDSVDFSSLTKDEMVQTLKKLINEKPIQKIKAEVESIKSIFYR
                     KHKLEVERLRREFVEAGGDVAGFTPPEDDAEIQIKELLKLYREKRAQYNGELEVQKKQ
                     NLELKMQIIEQIKDLVNSSEAVNVTFQEFRNLQQKFREVGPVPQANLNDLWENYHHHV
                     QNFYDYIKINKELRDLDLKKNLEEKIKLCEKAEELLLEPSVVAAFKKLQKYHTQWREV
                     GPVPHENQNEIWERFKEATAKINKKHQEYFESLRDEQKRNLDAKAALCEKAEELASLV
                     IKTNKEWNQRSKEMVELQKVWKTIGFAPKKDNNKVYERFRAACDTFFNNKRDFYSETK
                     EDQLNNLQLKTELCIQAESLKDSTEWKKTTEDFINLQKRWKEIGPVPRKNSDEIWKRF
                     RAACDYFFTQKSSHFSQVDNSYDKNLKDKEMLIAEIAAYQLTDNPEADLLALKEFQRR
                     WAEVGFVPIKNKEDIQKRYREAINKHFETLNIGEEKRNMFNFKTKLESATGNPRQENK
                     LRQERDKFFNRLRQLEGDIALWENNIGFFTRSKNAEAMIKEVERKIEKAKEEIRILEQ
                     KIKMIDSMDSK"
     gene            complement(5777..6226)
                     /locus_tag="PK502_09120"
     CDS             complement(5777..6226)
                     /locus_tag="PK502_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPH01016.1"
                     /translation="MTLVTFNSPLTSGRPSGTSVVESIFDALRDTAMNLAEVSASYPA
                     VNIFEEPERFRIEVAAPGYKKEQFNIQVDANDVLTLSVNEGKNNVEGESYLRREFANG
                     SFERHFTLGEMIDANKIEATYRDGILTIVIAKKEEAKPQKPRTISIS"
BASE COUNT         1468 a         1463 c         1434 g         1988 t
ORIGIN      
        1 tctggtcgtt actcattttg gaagttatga ccattgagat tgccatgagt agggcaaaga
       61 ggctgatgtg atcgccaaac catgggatgg tgaagggcag gtggagaatg gagtcgtatg
      121 acgaaaggtc ttcggcccat agaaagctct gctgcctgag ttcgaatgat gcggggaaga
      181 agcgaaacat ggcaatgaga atggggaatt ggatgaggac aggaaggcag cctcccattg
      241 ggctcacccc aacctttttg taaagcgcca tcacagcttg ctgcttcttc atggcgtcct
      301 ccttcttggg gtacttcttg ctgatttcgt caatctgggg cttcaaaacc ctcatcttgg
      361 ccgagctgag gtacgacttg taggtgagcg gcagcagcac cagcttgatg atgatggtca
      421 ttatcagcac aatgatacca tagttggtta gaaaactgct taaccagtag aatagcagaa
      481 tgatgacgta cttgtttatc cagcggacaa tatttcgtcc cagcggcacc actttttcaa
      541 acccctggtt gtattccttt agggttttgt agtggttggg gccaaagtaa aagcccaact
      601 tcaaggtttc gtcgttctga tttttaaaag ggagcgataa ccgggcatcc atggccttga
      661 cgtgcaggct atcgttcagg ttggtcatct ttaggtcgga gttgatgaag tgctcattgg
      721 caaccagtat ggacgagaaa aactggtgtt tgaagtcaac ccattttaac ttggtggtta
      781 gttctttctt ttcgtcctgg ctgcgcgggt ttagctcctc atagtcgttg ttcaggaagg
      841 tgtaggctat ggtggtgtag ttacgttcat tctctattcc cttttccagc ctgttgagct
      901 tgaggttcca caggagatca attgaaccgg cttgtccgcc aatggacttg gtcatcccgt
      961 taaagcggat gtcaaaatca accatgtagc tgttgggctt aacggtgtag atgtagtcga
     1021 tgtaggagga atcggcaatt tgaagtcgca tggttagtga ggccgattgg ctttccgatg
     1081 cggtaactgc ggtatcggag gtttggggaa caaagaagag gtcattagtt ttaacggcgt
     1141 tgttttcacc ccagaactgt aggccaaatt cgttgtcatc gccgttaaaa agtaccagtg
     1201 gctcaccggc atgggtttta aactttttta gctcaacgga gtatattctg ccacccctat
     1261 tcgacaccac cacgcgcatg aggtcgtttt ccaggcttat gaacttgcgt tcaccttcca
     1321 gggcaggggc aaatgagcct acctgggact gaacttcctt tttctgaatg gtgtcagcaa
     1381 gcatggcagc cttagccttt tcctcttcgg ctcttttctg ttcatactcg gcttgcaccc
     1441 tggctaacga atcggcttta cgtacgtagg cttcacgctc ttcttttgaa ggtgcggcaa
     1501 ggtacccaaa gccaatcatg attaagaaaa taagcactaa acctaatatt gtattcctgt
     1561 ccatctgctg ctgattttct ggttgtttaa gtgaaaaaaa agccatcctt ttagggtggc
     1621 aaaggtagta atatttggat ggttagccac caaaattcat ggtggcttta acaaattcaa
     1681 caaaaagcgg atgcgggtta ataaccgtgc ttttatactc cggatggaac tgcaccccaa
     1741 cgaaccacgg atgagaagaa atttccatta tttcaaccag gttggtttct ggattaatgc
     1801 cggctgcgac catgccggct gcctcgaact ggggcaaaaa ctggttgtta aactcgtagc
     1861 ggtgccggtg cctttcgtgg gtttctagtt tatgataggc catgtaggct ttagttccct
     1921 tttttacctg gcaggggtag gagccaagcc gcatggtgcc acccatgtcg gtgattccct
     1981 tttgctcctc catcaaatca attaccggaa agggcgtatt gcgcaccatt tcggtagagt
     2041 tggcctcctg caagcccaga acatttctgg caaactcaac tacagcacac tgcattccca
     2101 ggcaaatgcc aaagaaaggg acttgattct ctctggcata tcgaatggct tcaattttgc
     2161 cctcaactcc cctgtgacca aaaccgggcg ctaccagtat gccctgcagg tggcccagct
     2221 tctcttttac atttgatgcg gtgatgcttt ccgattgaat catttcgagc tcaacatggc
     2281 attcgtttac cgctccggca tgaataaacg actcaacaat tgatttatag gcatcgtgta
     2341 gctcaacgta ctttccaacc aacccaactt ttactttcga cttcggattt ttaagcttgt
     2401 ttataaactc gttccaatcg tcaagtttgg gaggttcctg aacaggcagg ttgagttttt
     2461 tcatgatggt ctcgtcgagt ttctccttat gcataagtag cggaacctca taaatagttt
     2521 ttacatcaat agactcaacc actgccgagg aatcaacgtt gcagaatagc gctaccttgc
     2581 gacggatgtc atcatttaag ggtttctctg ttcttagtac cagaacatcc ggttggatac
     2641 cattttcaag aagcacttta acggaatgct gggtgggttt tgttttaagt tcacccgaag
     2701 ctgacaggta gggcaccagt gtaagatgga tgaatgcgga gttggttgga ccaagctccc
     2761 acctaagttg gcgcaccgct tcaatgtaag gtagcgattc aatgtcgcct actgttccac
     2821 caatttcggt tatcaccaca tcgagcttat gcttcgttcc aaggagtttt attcttcgct
     2881 ttatctcatc ggtaatatgg ggaataacct ggacggtttt acccaggtaa tcgccgtgcc
     2941 tctccttgct tataacagtt tggtaaattt taccggttgt aacgttgttc atctgggatg
     3001 ttgggaggtt taaaaacctc tcgtagtgtc ccaggtcaag gtctgtttct gccccgtcct
     3061 cggttacgta gcactcgcca tgctcgtatg ggtttagagt tcccggatct acgttgatgt
     3121 atggatcgag tttttgaatg gttaccgaga aacctctgct ttgtagcaac ttcccaagtg
     3181 aagctgatat tatccccttt cccagcgatg aggcaactcc gccggtaaca aaaacatact
     3241 tggtgttgtg tggcatggct tgaacttgtt ttaatctagc ttaacagaaa aattcaagga
     3301 taaaggataa aaaataaaat aaacttgtcc gcctccgggc ggaacttgtc cgcctactgg
     3361 cggattcaaa atttcatttt caaacttttt acctttaacc aaaactgctt ctacagttca
     3421 cagctaataa ctgttttgca tggttgaaat gcaccgcttt tgctacttac tgtccataga
     3481 gtcgatcatc ttgatttttt gttccaaaat ccgaatttcc tccttagctt tttcaatttt
     3541 acgttcaacc tccttaatca ttgcctcggc attttttgaa cgggtaaaga acccgatgtt
     3601 gttttcccat agagcaatgt caccctccag ctggcgcagg cggttgaaga acttatcgcg
     3661 ctcctgccgg agcttatttt cctgtctggg gtttcctgtt gcgctttcta gcttggtttt
     3721 aaagttgaac atgttgcgct tttcctcgcc aatattaagc gtttcgaagt gcttgttgat
     3781 agcctcgcgg tatcgtttct gaatgtcctc cttatttttt attggcacaa agccaacctc
     3841 ggcccagcgg cgctgaaatt cctttaaggc cagcaggtcg gcttccggat tgtcggttag
     3901 ctggtaagcg gcaatttcgg caataagcat ctccttatcc ttcaggttct tatcatagct
     3961 gttatcaacc tgggagaagt ggctggactt ttgggtgaaa aagtagtcgc aagctgcacg
     4021 gaagcgtttc caaatctcat ccgaattttt acgtggaacg ggtccaatct ccttccagcg
     4081 cttttgcagg ttgataaagt cctcggtggt ttttttccac tccgtgctat ccttcaggct
     4141 ttcggcctgt atgcaaagtt cggttttaag ctgcaggtta tttagctgat cctctttggt
     4201 ttcgctgtaa aaatcgcgct tattattaaa aaaagtgtcg catgccgccc tgaacctttc
     4261 gtaaaccttg ttgttgtcct ttttaggcgc aaagccgatg gtcttccaaa ccttttgcag
     4321 ctccaccatc tccttcgagc gctggttcca ctccttgttg gttttgataa ccagcgaggc
     4381 aagttcttca gccttttcgc aaagggcagc tttggcgtca aggtttcttt tttgctcatc
     4441 cctgagcgat tcgaagtact cctgatgttt cttgttaatt ttagcggttg cctccttaaa
     4501 cctttcccaa atctcgttct gattttcatg tggaaccggg ccaacttccc gccactgggt
     4561 atggtacttt tgcagctttt tgaaagctgc tacaaccgag ggttcgagca ggagctcctc
     4621 ggctttttcg caaagcttaa tcttctcctc gaggttcttt tttaggtcaa ggtcgcgtag
     4681 ctccttgttt atttttatgt agtcgtagaa gttttgcaca tgatggtggt aattttccca
     4741 cagatcgttc aggtttgctt gcggaacggg cccaacttcc cggaactttt gctgaagatt
     4801 tctaaactcc tgaaaggtaa cgttaaccgc ttcggagctg ttcactaaat cctttatttg
     4861 ctcaataatt tgcatcttaa gctcaaggtt ctgtttcttc tgaacctcca gctcgccgtt
     4921 gtattgtgcc cgcttttcgc ggtatagctt gagcagttct tttatttgaa tttccgcatc
     4981 atcttctggt ggtgtaaaac ccgccacatc accgccggct tcaacaaact cgcgtcgtaa
     5041 acgctcaacc tccagcttat gcttacggta gaatatgctc tttatgcttt caacctcagc
     5101 cttgattttt tggatgggct tttcgtttat gagttttttg agggtctgaa ccatctcatc
     5161 ctttgtgagg ctcgaaaaat ctacactgtc aattttaatt tcctcacttt cctcatcaac
     5221 ctcttcatca ccatcttcga tatcttctac ctcttccagc tcctcgcttt cggcgggtag
     5281 ttcaatctca ttaacgggga tgttggttgc ttcagcagac tccccttcgg attttacggt
     5341 tgtacttgca tcgaaatgga taccctgggg atcatccccc agctgattgg aaatctcgat
     5401 acgctctagc gcaacaggct cgttttcctg ctcggctaaa ctggaagaat ccatcgtacc
     5461 ctcgctattt gcaaggtttt gatccttagg atcagtaaga tcgttggtgc tcatggtaat
     5521 ctcgttatta ttttctaaag acatacaaac gtatggctgg gtttaacgtg atttgcgaaa
     5581 atatgcaatt catcccatcc tcaaattatt ttcgtcaaaa gtattgattt ttgaggatta
     5641 cccaaaattg gtataaaatt acaggttgac ttttatgcaa aatgcttccc aatgcagtag
     5701 ggaaagcagg aataaaaaaa acctaatgga gaaaacgcca ttaggtttat gtgttgctat
     5761 ttgtatcaac ttgcggttag ctgatgctta tggttctggg tttttggggt tttgcctctt
     5821 cctttttggc gatgacgatg gtcaggatgc catcacggta ggtggcttca attttatttg
     5881 catcaatcat ttcgcctagc gtaaaatggc gttcaaatga accattggca aactcacgac
     5941 gcaagtacga ttctccttca acattgtttt tcccctcatt cacgctaagg gtaagcacgt
     6001 cgttagcgtc aacctggata ttgaactgtt cctttttata gcctggtgca gcaacctcaa
     6061 tacggaacct ctcgggttcc tcgaaaatat tcactgccgg atagctggcg ctcacttctg
     6121 ccaggttcat ggccgtatct ctaagggcgt cgaatatgct ttcaactacc gatgttccac
     6181 ttggcctccc agaggttaat ggactgttaa aagttaccag tgtcatagct tttctgtttt
     6241 attgtgttga tttaacgttt cttaatctac ttccaacttt atgccaaacg aagcaatcgg
     6301 ccatattgac gtataatgaa gtccgaatat gtcataatgg cgttttgcgc gtc
//
LOCUS       DAOPHX010000134         6282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_16211_length_6282_cov_5.089128, whole genome shotgun sequence.
ACCESSION   DAOPHX010000134 DAOPHX010000000
VERSION     DAOPHX010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16211_length_6282_cov_5.089128"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..587)
                     /locus_tag="PK502_09125"
     CDS             complement(24..587)
                     /locus_tag="PK502_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01017.1"
                     /translation="MFDDYKYKKALNEINRQAQGFKRQRMVHNLTTARKILIVFTLNS
                     QEVLDSVLNLVKYFESLNITVHALGYYPQKEVPQVFTMHPGITIFSKAETNWYGKPLS
                     SDVEAFINKEYDILIDVDFDEVVPVRWISTFSKAKFKLGSQNYLNNPFDLILTVDKLK
                     GLPYLCEQVKQVLFKLNNRFADEPHQA"
     gene            complement(834..2855)
                     /gene="ligA"
                     /locus_tag="PK502_09130"
     CDS             complement(834..2855)
                     /gene="ligA"
                     /locus_tag="PK502_09130"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPH01018.1"
                     /translation="MTAEEKDRVQQRIMFLRDELNRHNYLYYVKTAPEISDFDFDMML
                     NELVELEKQYPEFDDVNSPSRRVGSDSSAEFKQVQHRYPMLSLANTYSEGELRDFDNR
                     VRNALSEPPTYVCELKFDGVAIGLTYLDGRLVQAVTRGDGSAGDDVTANARTIRTIPL
                     KLHGSDYPREFEIRGEIIMPRAIFDELNREREDEGEQPFANPRNAAAGTLKLLNPSAV
                     AKRRLDSFFYYMLGEELPFDNHYQNLMAAKEWGFKISEHIQLCSNLECVFDFIKYWDL
                     ARKQLPFDTDGVVVKVNSYRHQAILGMTAKTPRWAIAYKYKPEQVSTLLLSVDFQVGR
                     TGAITPVANLKPVKLAGTVVKRASLHNADQVALLDVRIGDWVYVEKGGEIIPKIVGVD
                     LSKRSPLAKILEFPTHCPECGSKLVKIEDEARHFCPNQFACPPQIKGRIEHFISRKAM
                     NIDGLGEETVALLYDNGLVKDAADLYFLKKDQLLLLDRFAHKSAENAIRSIEKSKNVP
                     FYRVVYAIGIRYVGEATARSLAQHFGSMEAIAKATYNELVEVEDIGDRIAQSIIEFFS
                     NPANRQLVEKLKMAGLKLQVEQNENLQLSDALKGMSVVISGTFSKVSRDELKELIRQH
                     GGKNVSSVSASTSLLIAGNNIGPSKLEKATKLGVKIVGEDEFFKLIGQE"
     gene            3044..5212
                     /locus_tag="PK502_09135"
     CDS             3044..5212
                     /locus_tag="PK502_09135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HPH01019.1"
                     /translation="MKTNAIADKYRDLLRVLRGSCGKDELKKIRKALDLVVSLNSAST
                     QPASSTILHLLEVARICVGEIGLGAQAAIAAILHETLENKQVTRVEIEKHFDSKVAEI
                     VEGYTLISGLDPKDPKTQAELFRQMLITLSGNAVVILIKLADRLETMRSLSRAEPEVR
                     QKRSWETFHLYAPLAHRLGLYPIKSEMEDLAMKHIYPKEYKFIVKKLRETTSSRNRFI
                     RSFIEPIEQELKNRHFDFEIKGRPKSVYSIFRKMQKQDVDFEDVYDVFAIRIILNTDP
                     EHEKSDCWQVYSIITDKYTPNPERLRDWISAPKSNGYESLHTTVLGPEGKWIEVQIRT
                     ARMDEIAEKGFAAHWKYKGIKQEQGVDEWVSMVRNILESPDGSFGDKTDLLQLNLNSK
                     DIFVFTPKGEIRKLPSKATVLDFAFDIHSEVGSKCTGAIVNGKNAPIKQVLQNGDIVE
                     VLTSKKQKPKKDWLNFVTTSKAKTRIKQQLREVENQEIARGREMLLRRLKNWKVDNPE
                     EAIRNINKQLKLKDPTEIFVLIAEGKVNLTSIKDILTSNIEPEAPKNVDATTAPARPT
                     DTTGSSSEFLIIDERLVNIDYKLAKCCNPIFGDEIFGFVTVKDGIKIHRQSCPNATQL
                     NTRWGYRIVKARWRGSSNDGAFQTSIMVTGMDELGMLNRISEIISKDLRVNMRNLNVN
                     SKNGQFEATIQLYVQDKKHLEMLLYRLGAIPGVSKAERLR"
     gene            5319..5951
                     /locus_tag="PK502_09140"
     CDS             5319..5951
                     /locus_tag="PK502_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01020.1"
                     /translation="MSRKSLILFTLLSLFGMAARGQLVQDLYKIYDPVFGNDDHDSTA
                     ILTPFGVIKPGVSYNLSVGTSYSSFGSGMGLSETYISPRVSYSPNQKLQVVAGISFSR
                     TGTHGLSTPSPTNISTGQALDNPYQAWAYTQYNFNNRFSVYAMGSVSQNQTYFSPYSR
                     TLGSYNSQMYGVGFNYRISSKASIGASFNFVNRNPFGYQLGGYSNPFIPY"
BASE COUNT         1828 a         1453 c         1379 g         1622 t
ORIGIN      
        1 cataatagtc ggataagcta gttttaagcc tgatggggtt catcggcaaa acggttgttg
       61 agcttaaaga gaacctgttt tacctgctca cagaggtatg gcaatccctt aagcttgtca
      121 accgttaaaa tcaaatcaaa cgggttgttg aggtagtttt gagagcccag cttgaatttc
      181 gctttagaaa atgtcgaaat ccatcgtact ggaacaacct catcaaaatc tacatcaata
      241 agaatatcat actccttgtt aatgaaggct tcaacatcgg aggataaggg ttttccatac
      301 cagttggttt cggccttact aaatatggtt atccctgggt gcatggtgaa aacttgtggc
      361 acctcctttt gggggtagta gcctaaagcg tggacagtta tgtttaaact ttcaaaatat
      421 ttaaccaggt ttaacacgct gtcaagaact tcctgactgt tgagggtaaa tactatcaaa
      481 atcttacggg cagtagtgag gttatgcacc attctctgcc tcttaaaccc ttgagcttgc
      541 ctattaatct cgttcagagc ttttttgtac ttataatcgt caaacatacc ttcgcattct
      601 agcttatcaa taataataaa agtgctatta acccgctact taattctaaa actcggcaaa
      661 tttagaagga taatctaaaa ggcaatttta caaaaaaatg ggttgataat caaatttaac
      721 aaagctttac aaagtcggag ggcaaccgct tgaaaattga caaccctgaa gcttccaatc
      781 ccaaaatagt ttagtgcagc caggttgttg cctcccccca aaggtagaat gcgctactct
      841 tgaccaataa gcttaaaaaa ctcatcctcg ccaactattt taactcccag ctttgttgcc
      901 ttttcaagtt tagaaggtcc aatattattg cctgcaatta aaagggaggt ggaagccgaa
      961 acgctgctta catttttccc gccgtgctgg cggataagtt cttttaactc gtcgcgcgaa
     1021 acttttgaaa atgttccgga aatgactacc gacattccct taagcgcatc cgaaagctgg
     1081 aggttttcgt tctgctctac ttgaagtttt aatcccgcca tttttagctt ctcgacaagt
     1141 tgcctgttag cagggtttga aaaaaattca atgatgcttt gggcaattct gtctccaata
     1201 tcctcaacct caacaagttc gttgtaggta gccttggcaa tagcctccat gcttccgaaa
     1261 tgttgtgcca agcttcgtgc tgtagcttca cctacgtatc gaattccaat ggcgtaaaca
     1321 accctataaa agggtacgtt tttagatttt tcgatacttc taatggcatt ttcagcagat
     1381 ttatgggcaa aacggtcaag caataaaagt tgatcctttt tgagaaagta caggtcagca
     1441 gcatctttta ccagaccatt gtcgtaaagc aaagcaacgg tctcctcccc tagcccatca
     1501 atgttcatag ccttacggct tataaaatgc tctattctgc ccttaatttg tggtggacag
     1561 gcaaactggt tggggcaaaa gtgccttgct tcatcctcta tttttaccag cttagaaccg
     1621 cattcggggc aatgggtagg aaattcgagt attttggcta aagggctacg ttttgacagg
     1681 tcaactccaa caatcttggg gataatttca ccgcccttct caacataaac ccaatcgccg
     1741 atgcgaacat ccaacagggc tacctgatcg gcattgtgaa gcgaagcacg cttaacaaca
     1801 gtgccggcca gcttaacagg tttaaggttg gccactggag taatggcacc tgtgcggcca
     1861 acctgaaaat caacagaaag cagtagggtt gaaacctgct cgggtttgta cttgtaggca
     1921 atggcccagc gtggggtttt ggcagtcatt ccaagtatag cctgatggcg gtagctgtta
     1981 acctttacca ccaccccatc ggtatcaaag ggcagctgct tacgcgccaa atcccaatac
     2041 tttatgaaat cgaatacaca ctccagattc gaacaaagct gtatatgctc ggaaatttta
     2101 aacccccact cctttgcagc catcaagttc tggtaatggt tatcaaaagg caactcttcc
     2161 ccaagcatat agtaaaaaaa actgtcgagc ctgcgcttgg caacagctga agggttgaga
     2221 agctttaggg tgcctgcagc agcattccgg ggattggcaa aaggctgctc tccctcatcc
     2281 tcccgctcac ggttaagctc atcaaaaata gctcgcggca taatgatctc acccctaatt
     2341 tcaaactccc tcgggtaatc cgaaccatga agtttcaagg gtatggttct aatggtgcga
     2401 gcgttggcag taacatcatc acctgcagaa ccatcgccgc gggtaacagc ctgcaccagc
     2461 ctgccatcga ggtaggtaag gcctattgcc acgccatcaa attttagctc gcaaacgtag
     2521 gttgggggtt ccgaaagggc atttcgcacc cggttgtcaa aatcgcgcaa ctcgccttca
     2581 gaataggtat ttgccaacga cagcatggga tagcgatgtt gtacctgctt aaactcagca
     2641 ctcgaatcgc ttcccaccct acgcgagggc gaattgacat catcaaactc gggatactgc
     2701 ttttcgagct ccaccagctc gtttaacatc atatcaaagt caaaatcgct gatttccgga
     2761 gcagttttca cgtagtagag gtagttgtgg cggttaagct catcacgcaa aaacatgatg
     2821 cgctgttgca ccctatcttt ttcttctgct gtcattggtt tgtctttaag cttatgacgt
     2881 taacgctaat tatttttcaa atatacttga gaaattaaaa ttatgaacag ggaaagttag
     2941 gctagcgctg taaaacacct caggttgaat atacgaaaat taagtatttg caaatttata
     3001 tatttacggg gtataaacac atgctaatta aacatcttac aagttgaaaa ccaatgccat
     3061 agcagataag tacagggact tactccgggt tttacggggt agctgtggaa aggatgagct
     3121 gaaaaaaatt aggaaagcgc tggatttggt tgtcagctta aactcggcca gcacgcaacc
     3181 tgccagctca actatacttc acctgcttga ggttgcccgg atttgtgttg gcgaaattgg
     3241 gcttggagcc caggctgcca ttgctgctat acttcacgaa acactagaaa acaagcaggt
     3301 aacaagggtt gaaatagaga agcattttga ttcgaaagta gccgagattg ttgagggtta
     3361 cacgctgata tccggacttg acccaaagga tcctaaaact caggcagagc tgttcaggca
     3421 aatgctgata accctatcgg ggaatgctgt ggtaattctc atcaagctgg ccgaccgcct
     3481 tgaaaccatg cgctcgctca gccgtgcaga gccagaggtg aggcaaaagc gctcgtggga
     3541 aacatttcac ctgtatgctc cacttgccca taggcttgga ctttacccca tcaaatcgga
     3601 gatggaagac ctggctatga agcacatcta ccccaaggag tacaagttca tagttaagaa
     3661 acttagggaa accacttcaa gccgaaacag gtttatccgt agctttattg agccaattga
     3721 gcaggagctc aaaaatcgcc atttcgattt cgagataaag ggtaggccaa agtcggtgta
     3781 ctccatattc cgcaagatgc aaaagcagga tgttgatttt gaggatgttt atgatgtttt
     3841 tgccattagg ataattctta acaccgatcc cgagcatgaa aagtcggact gctggcaggt
     3901 ttactccatc atcaccgaca agtacacccc caatcccgaa cggctacgtg attggatttc
     3961 agcgccaaag agcaacggct acgaaagcct tcacacaacg gtgcttggcc ccgagggcaa
     4021 gtggatagag gttcagattc gcaccgcacg aatggacgaa atagctgaaa agggatttgc
     4081 agcccactgg aagtataagg ggataaaaca ggagcagggg gttgacgaat gggtaagcat
     4141 ggttaggaat atactcgaat cgcccgatgg ttcttttggt gataaaactg acctgctgca
     4201 gctcaacctt aacagcaagg atatatttgt atttaccccc aagggggaaa taagaaaatt
     4261 acccagcaaa gccaccgttc tggactttgc ctttgacatc cactccgaag tggggtcgaa
     4321 atgcaccggt gccattgtga atggcaaaaa tgctccaatc aagcaagtgt tgcagaatgg
     4381 cgatattgtt gaagtgctca cctcaaagaa acaaaagccc aaaaaagact ggctcaactt
     4441 tgtaaccacc tcaaaagcaa aaacccgaat taaacagcag ctccgggagg ttgaaaatca
     4501 ggaaattgcc aggggtaggg agatgctgct tcgccggctt aaaaactgga aagttgacaa
     4561 ccccgaagaa gccattcgca acatcaacaa gcagctaaaa ctaaaagacc caacagaaat
     4621 tttcgttctg atagccgaag gtaaggtaaa ccttacctcc attaaagata tactcacctc
     4681 aaacattgaa cccgaagccc ctaagaatgt tgatgccact actgcacctg cccgccctac
     4741 cgacacaacc ggcagctcat cggaatttct gataattgac gagcgactgg taaacattga
     4801 ctacaagctg gccaaatgct gcaatccgat ttttggggat gagatattcg gtttcgttac
     4861 ggtgaaggac ggcatcaaaa ttcaccgtca aagctgcccg aatgccaccc agctgaatac
     4921 ccggtgggga taccggatag ttaaagccag atggagaggt tcttcaaatg atggggcatt
     4981 ccagacctcc atcatggtta caggtatgga cgagctgggg atgctaaacc ggatatccga
     5041 gattatcagc aaggatttga gggtaaacat gcgcaacctc aacgtcaact ccaaaaacgg
     5101 acagtttgaa gctaccatcc agctctacgt tcaggataaa aagcatctcg aaatgctgct
     5161 ttaccggctt ggagccattc caggggtaag taaggccgaa aggctaaggt agcaaagctg
     5221 ccgagttgca attgcttaaa gttagctaat ttggagcggt tgcatttcaa ctctcccata
     5281 cttttagtat atttggatgt tcataaattg gtaaggctat gagcagaaaa tcgttgattc
     5341 tatttaccct actaagcctg tttgggatgg cagcaagggg acagctggtt caggatctat
     5401 ataaaatcta cgaccctgtt ttcggtaacg acgaccatga ctctacagca attttaactc
     5461 cttttggagt tataaaaccc ggtgtaagct acaacctgtc agttggcacc tcctactctt
     5521 cgtttggcag cgggatggga ctatctgaaa cctacatatc gcctagggtg agctactcac
     5581 ccaaccaaaa gttgcaggtt gtagccggaa tttcctttag ccgaacggga acgcacgggt
     5641 tgtcaacacc ctcccccacc aacatcagca ccggtcaagc ccttgacaat ccataccagg
     5701 catgggccta cactcagtat aacttcaaca accgatttag cgtttacgct atgggctcgg
     5761 ttagccagaa tcaaacctac ttctccccat attcccgaac gctggggagc tacaacagcc
     5821 agatgtacgg tgttggcttt aactaccgca taagtagtaa ggcaagcatt ggtgccagct
     5881 tcaacttcgt taaccggaac ccctttggct accagctcgg tggatatagc aaccccttta
     5941 ttccctacta gaaaaattaa cggttaaaga agccgaaaca tagggcaaaa caattttccc
     6001 caggttgcta ctatccaaag tattcatcat tctaagacca gtcgttccga acacctgctc
     6061 taagctggtc cactattggt ttatccggtt gctacatggc cataaacgtg gtcaagctac
     6121 agatgcttac acccaagcga gccagaaaag tttgcaggaa attagaaacc tatttgatga
     6181 gttataacat gtacactaca tttgtgtaca tacaactttt atagtactga taccattaaa
     6241 attggtgttt ttatacgacg atgtagtatt gatatgtaag tt
//
LOCUS       DAOPHX010000135         6250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_16327_length_6250_cov_6.517998, whole genome shotgun sequence.
ACCESSION   DAOPHX010000135 DAOPHX010000000
VERSION     DAOPHX010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16327_length_6250_cov_6.517998"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..3099
                     /locus_tag="PK502_09145"
     CDS             376..3099
                     /locus_tag="PK502_09145"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPH01021.1"
                     /translation="MKRLLTTVLSLFLLGMGSLYAQNTLRGVVKDSDTDTPVQDVRVR
                     LLGTTFETLSNAGGEFTLEGVPDGKFTLEFSIVGYQTITQEVSLPENSQVPVVSLRRE
                     VVADNGSQEFSEVVISADDLDSDNMDQAISGLLQSSQDAFNSAVSYTFSPASFKIRGY
                     DSDYSLLYMNGVPVNDPEIGFASWSSWGGLNDVVRSRESRNGFLPTNFSFGGLGGATN
                     LNVRASQQRVGTRLSYAATNRTYNNRIMFTHSTGVMENGVSFTISGSRRWAEEGYVDG
                     TNYDAWAYFIGIEKKFNSQHSVAFTTYNAPTKRGMQGVSTEEAKNLAGSSYYNPNWGY
                     QNGEKRNSRVRFQQEPTFILNHYWNMLPNLALTTTAGYSFGTYQTTALNWYDAQDPRP
                     DYYRKLPSYWIDSDPDVSQAIADAFSNDPAISQIDWGYLYQVNYNSLDTASNLLRSKY
                     IVENRITDSRQFSLTSVASWNPTQEMKVNGGIEMTLYKGRNYKQIDDLLGGNYWLDID
                     QFVERDYFDNANLAQNDLDNPNRMVAKGDVFGYDYTANVNNGNLWAVANYTLNRFEYY
                     LGGSYTYTEFWRTGDMRNGRFPSNSKGDSPKQQFNDFGVKGGATWKISGRHYLDANMA
                     YLTRAPFFRNSYISPRTSNFVIPGLGSEKIASGDINYNLRTPYIKARITAYYTRFYDQ
                     TELKSFYDDYYRTFINYTMRNIDKEHKGFEIGAEIKASPTLTVSTAAALGSYQYKSRP
                     LATITQDNLGSVLAVDQVVYIKNFFVPNTPQTAAMVGLRYASPKYWFLGATFSYFDDI
                     YIDFAPGKRTAEALVGLEPNDPRRYDLTHQESVSSAYLLDANIGKSWRIKNYYINLNF
                     QVANILDNTDFKTGGFEQARYSIAEQTSDKFPPKYFYAYGRTYYLIFGFRF"
     gene            3131..3997
                     /locus_tag="PK502_09150"
     CDS             3131..3997
                     /locus_tag="PK502_09150"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HPH01022.1"
                     /translation="MKLTKYFAATVVLIAMVTLSLPGCVDFSFDDPQPSIDSTSMSAN
                     TTIAELKSVYPGSLFQLSDTTFYLRDSIIIEGTVISDDKEGNFYKSIFIEDSTGGIEV
                     KLNKTTLYNDYKRGQKVVVLCNDLYLGDYGGQIQLGSTYKNNGVVEISGLEGDPIIRE
                     HVFKKGKTIQERIPTVLSVTQLNPTYIGMLVKFENVQIRDTISPITREIYTYADNVNK
                     VTVNHSIHQCGQAFDKLVLRTSGYARFASKPIQTKNGSITGVLTYYNGTYQLLIRDTN
                     DVDFTSNRCSNK"
     gene            4012..6078
                     /locus_tag="PK502_09155"
     CDS             4012..6078
                     /locus_tag="PK502_09155"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HPH01023.1"
                     /translation="MIKKILAILVPIAMVGLIALQNGCVKEDFDTVPPLRKAISWKGT
                     ATIADVKGFFNGPNNPSRTAGLIKKLVPASYWEQLAANGVGDSSIIIDGLVISCDSAA
                     NFYETVTIMDSTGGIDFKINASDLYLTYGFKPGKRVLVKLNDLAIDSYHGVYQIGLPY
                     TDAGTLKVTGVDPSMVAACTQMSGEQANLTPLSVDIGTITAGYVQKLIVIDSVQFWNP
                     EATYSLPGVNTNRTLVDTKGNQIVLRNSGYSKFVNDKVPSGSGYIVGVMGIYDGTYQL
                     TIRDPRDIQFNNPRFGSQPPAKNATIADLKTACTSNLVKITQDFVVEAVVLANDKSGN
                     IYKSLFIEDESGAIEFKIDVYDLFVDFPVGTKIAISCKDMYVGKYGGVVQLGGVYNNA
                     IGRISANEFYQKVFVVGSGFEVTPIPVNINELTADLVGKLVTISDVQYIDSELGKTYA
                     ESSITNRHVEDALGRTAIVRTSNYADFAGITLPSKRGSITAVLSKYYNDYQLYIRDLT
                     DVWMTQPRRVKNYILKQDFSSATANSPISVGGWQSIAEVGTRRWMAKVYSGNTYAEMN
                     PYQSGEASNIGWLISPAIPVASGVTTYLTFNTQFAYWANSTLEVFVSENYDGANPASA
                     TWTKLSNAHIVTQSEGQNTWVSSGLVDISEFPGTIYLGFKYSGGGSIGQTTAFRVDDV
                     NVFTQQ"
BASE COUNT         1732 a         1488 c         1418 g         1612 t
ORIGIN      
        1 cctgagcctc ggaataaccc aaagccacaa gcatgagaaa tactgccagc acattcctta
       61 gaaataatct atacataatc aagtgatttt aggataaacg accgaacaaa gatagctata
      121 tccgtaaaaa aagatataat atttggcaac tgtataaaaa aatgttcttt ttttgcatca
      181 tataattcca acccgttggt taggttaaaa tttctgaaat ttgttggctt attaattttg
      241 ttgttcaccc cgataattgc tgtgtcagtg taccgccacc tatttttttg ttgttaatat
      301 ttctaaaaat aaaatgtaga taagtgaaac aattcataca tatcaatcaa caaccaaata
      361 aataacaaat tgtaaatgaa gcgattatta acaactgtgc tgtccttgtt tttgctgggt
      421 atgggcagct tgtatgcgca gaatacccta agaggagttg tgaaggattc cgacacggat
      481 actcctgttc aggatgtgag ggtgcgctta ttaggcacta catttgaaac gttaagcaat
      541 gctggcggtg aatttacgtt ggagggtgtt cccgatggca aattcacctt ggagttttcc
      601 attgtaggct atcaaaccat tacgcaggag gtaagccttc ccgagaattc gcaagtgccc
      661 gttgtttcgc tgagacgcga ggtagttgcc gataacggat ctcaggaatt tagcgaggtg
      721 gtgatatctg ccgacgatct ggattcggac aacatggacc aggcgatttc ggggttgcta
      781 cagtcgtcgc aggatgcctt taactcggct gtttcgtaca catttagccc ggccagcttc
      841 aaaatccggg ggtatgattc ggactacagc ttgctctaca tgaacggagt tccggtgaac
      901 gacccggaga ttggttttgc ctcgtggagt tcatggggag ggttgaacga tgttgtccgg
      961 agcagggagt cccgcaatgg ttttcttcct accaactttt cgtttggcgg gctgggcgga
     1021 gcaacaaacc tgaatgttcg tgcctctcag cagcgagttg gaacaaggct ttcctatgcc
     1081 gccaccaacc gaacctacaa caaccgcatt atgtttaccc actccaccgg agtaatggag
     1141 aatggcgtat cgttcaccat tagcggttct cgtcggtggg cagaagaggg ctatgtagat
     1201 ggaaccaact acgatgcctg ggcgtacttt attgggattg aaaaaaagtt taacagtcag
     1261 cactccgtag cctttactac ctacaacgct ccaaccaaga ggggtatgca gggtgtttcg
     1321 accgaggaag caaaaaattt agcaggttcc agctactaca acccaaactg gggataccaa
     1381 aacggggaga agcgcaactc acgagttcgc tttcagcagg agcctacttt tattcttaac
     1441 cattactgga acatgttgcc caacctggcg cttaccacta cggcaggcta ctcctttggt
     1501 acctaccaaa caactgcgct aaactggtac gatgcccagg atcctcgacc cgattactac
     1561 cgcaagcttc caagctactg gatcgattcc gatcccgacg tgtctcaagc cattgccgat
     1621 gcatttagca acgatcctgc tatcagccag attgactggg gctaccttta ccaggtgaac
     1681 tacaacagcc tggacactgc ctccaacctt ctccgctcaa agtacatcgt agaaaataga
     1741 attaccgatt cccgtcagtt ctcgcttacc tcggtggcca gctggaatcc aacgcaggag
     1801 atgaaggtga acggcggaat tgaaatgacc ctttataaag gccgaaacta caaacaaatt
     1861 gacgacctgc tgggtggaaa ctactggctc gatatcgacc agtttgttga gcgcgactat
     1921 ttcgataacg ccaacctggc ccaaaacgat ttggacaacc ccaaccgtat ggttgccaag
     1981 ggtgatgttt ttgggtacga ctatacggcc aatgtaaata atggaaacct ttgggctgtg
     2041 gccaactaca ccctgaaccg tttcgagtac taccttggcg gtagctacac ctacaccgag
     2101 ttctggcgta cgggggatat gcgcaatggc cgattcccct ctaactcaaa gggcgattct
     2161 cccaagcaac agtttaacga ttttggggta aagggtggcg caacctggaa aatttcaggc
     2221 cgtcattacc ttgatgccaa catggcttac cttacccgtg ccccattttt cagaaattcc
     2281 tacatctctc cgcgcacatc aaactttgta ataccaggat taggcagcga gaagattgcc
     2341 agcggcgata tcaactataa cttgcgaaca ccctacatta aagctagaat aaccgcctac
     2401 tacacccgtt tctatgacca aacggagctt aaatcgtttt acgacgacta ctaccgcacc
     2461 ttcattaact acaccatgcg gaacattgac aaggagcata agggttttga aattggggct
     2521 gaaattaaag catcgcctac ccttactgta agcactgcag cagcgctggg ttcataccag
     2581 tacaaatcaa gaccccttgc caccattaca caggacaacc tgggttcggt gctggccgtt
     2641 gaccaggtgg tgtatatcaa aaactttttt gtccccaaca ccccacaaac ggctgccatg
     2701 gtaggcttaa ggtatgcttc gccaaaatac tggttcttgg gagcaacctt tagctacttc
     2761 gatgatatct acattgattt tgctccgggt aaacgtacag ccgaagccct ggttggcctt
     2821 gagcccaacg acccccgtcg ttacgacctt acccatcagg aaagcgtttc gtcggcctac
     2881 ctgcttgatg ccaacattgg aaaatcgtgg aggattaaga attactacat taacctgaac
     2941 tttcaggtgg caaatatcct cgacaacacc gactttaaaa caggaggatt cgaacaggcg
     3001 cgctactcca ttgccgagca aacatccgat aaatttcctc ctaaatactt ctacgcctac
     3061 ggtcgcacat actacctgat ttttggcttt agattttaac tcaacttgta taataactat
     3121 gatgagaaca atgaaattga ctaaatactt tgcagcaacg gtagtcctga ttgccatggt
     3181 aacgctatcg ctgcccggtt gtgttgactt ctccttcgac gatccccagc ccagcattga
     3241 ttcaacctcc atgtcggcca acaccaccat tgccgagctt aaatcggttt atcctggctc
     3301 tcttttccag ctctccgaca ccaccttcta ccttcgcgat tctataatca tcgagggaac
     3361 ggtgatttcg gatgacaagg agggtaactt ctacaagagc atttttattg aggatagtac
     3421 gggtggaatt gaggttaagc tcaataaaac aaccctgtat aacgactaca agcgcggtca
     3481 aaaagttgtg gtgctctgca acgacctgta cctgggtgac tacggtggtc aaattcagct
     3541 aggctcaacc tacaaaaaca atggggtagt agaaataagc ggtctggagg gcgatcctat
     3601 tatccgcgag catgttttta aaaaggggaa aaccatacag gagcgtatcc ctaccgtttt
     3661 atccgttacc cagctcaacc ccacctacat agggatgctg gttaagttcg aaaatgttca
     3721 aattcgtgat accatttctc ccattacaag ggaaatatac acctatgccg acaacgtgaa
     3781 caaggtaacc gttaaccaca gcattcatca gtgtgggcag gcatttgaca aacttgtgct
     3841 acgaaccagc ggctatgccc gttttgcatc caaacctatt caaaccaaaa atggctctat
     3901 tacaggagtt cttacctact ataacggaac ataccaactt ttgattaggg atacgaacga
     3961 tgtggatttt acaagtaata gatgcagtaa caaatagagg aggaactaac gatgattaaa
     4021 aaaatactgg caattctggt gcccatcgcc atggtagggt taattgcact acaaaatggc
     4081 tgtgtaaagg aggatttcga taccgttcct ccattacgca aggcaatttc gtggaaggga
     4141 actgctacga tagctgatgt gaagggcttt ttcaacgggc cgaacaatcc ttcccgaaca
     4201 gctggactga ttaagaagct agtaccagcc agctactggg aacagcttgc tgccaatggt
     4261 gtaggcgatt cctccatcat tattgatggg ctggttattt cgtgcgattc ggcagccaac
     4321 ttttacgaaa cggttaccat catggactca accggtggaa ttgattttaa aatcaatgcc
     4381 agcgacctgt acctcaccta cggatttaaa ccgggaaagc gggtgctggt taagctgaac
     4441 gatttagcca ttgatagcta ccatggggtt taccagatag gattacccta caccgatgca
     4501 ggcacactta aggttaccgg cgttgacccc agtatggtgg cagcctgcac ccaaatgtct
     4561 ggtgagcaag ctaaccttac tcccttgtcg gtggacattg gcactattac agccggctat
     4621 gttcaaaagc taatcgttat tgatagcgtt cagttttgga atcctgaggc tacatacagc
     4681 ttgccgggtg ttaataccaa ccgcaccctg gtagatacga aaggaaatca aattgttttg
     4741 cgtaatagcg gctactccaa atttgtaaac gacaaggttc catccgggag tggatatatt
     4801 gtcggggtga tgggaatata cgatggcacc taccagctta ccattcgcga cccgcgcgac
     4861 atccagttca acaacccccg ctttggctct caaccacctg ctaaaaatgc caccattgct
     4921 gatttaaaga ccgcttgcac ctcaaacctg gtgaaaatta cacaggattt tgtggttgag
     4981 gcggttgtac tagccaacga caaatccggg aatatttaca aaagtctgtt tattgaggac
     5041 gaatcggggg caatagagtt taagatagac gtgtacgacc tatttgttga ttttccggtt
     5101 ggaacaaaaa ttgccatttc ctgtaaggat atgtacgtgg gtaaatatgg cggtgtggtg
     5161 cagctgggtg gcgtttacaa caatgccatt ggcaggattt ccgccaacga gttctaccag
     5221 aaagtctttg tggttggttc aggatttgaa gtaaccccaa ttcctgtaaa catcaacgag
     5281 ctaacagccg acctggtagg caaattagtt accatatctg atgttcagta tatagattcc
     5341 gagttgggca aaacatacgc cgagtcgagt atcaccaatc gtcatgtgga ggacgctttg
     5401 ggtagaaccg ctattgtgag aacaagtaac tatgctgatt ttgcaggtat tacccttcca
     5461 agcaaaaggg ggagtataac cgccgtgctg agcaagtact acaacgacta ccagctttac
     5521 attcgtgacc ttaccgacgt ttggatgacc caaccacgtc gggttaaaaa ctacattctt
     5581 aaacaggact ttagctctgc aaccgcaaac agtccaatta gcgttggtgg atggcaatcc
     5641 attgcggagg ttggaacacg cagatggatg gccaaggttt actcgggcaa cacctatgcc
     5701 gaaatgaatc cataccagtc gggtgaagcc agtaatatcg gatggctgat ttcccctgct
     5761 attcctgttg cttcgggcgt aacaacctat cttaccttca acacccaatt tgcctactgg
     5821 gccaactcca cgctcgaggt atttgtgtcg gaaaattacg atggagctaa ccctgcctcg
     5881 gcaacatgga caaaactttc caatgctcac atcgtaacgc agagcgaagg gcaaaacaca
     5941 tgggtaagct ccggcctggt tgatatttcg gagtttcccg gaactattta tctcgggttc
     6001 aagtactctg gaggaggttc tattggccaa accaccgcct ttagggttga tgacgttaat
     6061 gtttttacac agcagtaaaa ttatttagct gttactttgg tactcgaagg ccaaccggat
     6121 tatcggttgg cctttacttt aacccacaaa ttctaagata aagtataaag tggtttcaag
     6181 acttgtccgc ctcccggcgg aacttgtccg gctcctgacg gattcaagat ttcagattca
     6241 agattcaaaa
//
LOCUS       DAOPHX010000136         6131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_16774_length_6131_cov_3.680546, whole genome shotgun sequence.
ACCESSION   DAOPHX010000136 DAOPHX010000000
VERSION     DAOPHX010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16774_length_6131_cov_3.680546"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PK502_09160"
     CDS             <1..538
                     /locus_tag="PK502_09160"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPH01024.1"
                     /translation="GPSHMTNPIGYADCATHSDLLALYTSTDVRKTLFRTDPDGVSGS
                     FWTTKYKGKGDGDALGTPDANNVIVLRLSEMYLNRAEALVNGATIPGATALSDINAIV
                     TKRGLAPYSSVGADVVFLERRLELAFEGHLWFDYIRTNRPSTRSDKPLAADDYRWLMP
                     IPQREMDVNDNLVQNPEY"
     gene            555..1421
                     /locus_tag="PK502_09165"
     CDS             555..1421
                     /locus_tag="PK502_09165"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HPH01025.1"
                     /translation="MKRIRYILSLAVLAVLASCNPNEMPEFNDNDAFVAFDNASMSVE
                     ENAQTLSIPVTLASIKGISASVTYTLLDGGTAVEDSNFTFADANKTLTFDATNRTQYI
                     VVNIINKPGVFTGDLRFKLQLSDDGTIKPNAENVCTITITDLDHPLAAILGTWTATGT
                     SYFNGSESWDMELTKDPSDVTVVWFYPFVNGGSGYKIYGVVNDAKTEIRVPVGQEIAP
                     PGSYGFIELKGFYGPSGEEEIPDGGYITITIEPNKLSIKDEIGSYVWGDAGQTSGLGW
                     YNIFKADVVLTR"
     gene            1459..3135
                     /locus_tag="PK502_09170"
     CDS             1459..3135
                     /locus_tag="PK502_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01026.1"
                     /translation="MKLNMIKQIAAGLGVLSFAVVLYSCEADWEKDYKAVTLKAPATA
                     TVSVTSIADSTALLNYSLSVTGRLYVAVVPGTDETPAPDAQAILKQTVGGAVFSKQII
                     LNDAGALSSSVIVSGLVQNTSYKVFALPVNDDGVLGEIVTTEAFSTSDHHAPVLDLDS
                     GVNPAITGNASIDLNFKPVLTFDEPVVLAETFSIKMGYRNVVTAEIVWVDVPKDSITI
                     SGSKVTIAQPATAMNGQYVFLSIGNGSVKDRAGNSYEGVTSGIVGGYLEGIYWRIKFS
                     PTATQQILPADSVTSDVSMKITLDYPIKMRFPTSGEGGYVQGNVVVRYYTSTTTLDVQ
                     VPQANVAVVQDTLVQITLPRTPVYGETVTLKMAEGAMRNAYGNPSKAIAFGDKEWFIS
                     YGYTRNMVIGNYVIGNLVSFSGPITDEYNVITSAHPTDPNLVVISGLLGSEDDIVAEF
                     NGDFASFNIPTYDGMGQMISFPDGHPLADYILEVWNGYSSDGVATGFVNADGTIDMEG
                     LGFYAYDAVTLEALGWYDLFSQATWTPAGKSIVQQNYVLKLQNHKSKFVR"
     gene            3605..5275
                     /locus_tag="PK502_09175"
     CDS             3605..5275
                     /locus_tag="PK502_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HPH01027.1"
                     /translation="MKRIALLVLVFFVHINLFAVEVPQRVAKEHADGFMYKKLDKSIT
                     ESSIFPFQKGENRTYTYLVSYKPEGWAIVAADDRIPAIIAYSLKGSFDTVKIKEVPFY
                     FWFQHYANQIKEVVEGKETKRHASWDNHPFEAQKSTSPVEPLIGVEWNQSSGWNAYCP
                     ADSEGPGGHAYAGCVAVAMGQALSVYQHPEVGYGQNYYVDDTYGSLGAVFGDTQYKWD
                     SIMGLDQPNKYVALLLYHLGVSVDMNYGADGSGAFSSMVPGALKTFFDYTNSTTILKR
                     SNFTSAQWDSILVSELANGRPIYYSGDPGDGTAGHAFNIDGVDDQNRYHLNWGWSGAY
                     NGYYLITSLTPGSNNFSSNQEAVVNIMPRNHNPQDILLSNNSIMEGLPAYSLVGKVNV
                     LDETPGDSHTLEVIAPPTIDGEPGFVPFAILGDSLLTTEILNFQTRNRYEIIIKATDK
                     AGHSLEKSFFINVIKNTGVSDLTLSGEMIITPTNDGNLKVWLNNSAIGRATMNLYDIN
                     GKLIFSTTISKELQVLDTTIPLGNTLIKGLYVAVLNFGNEKISYKFVK"
     gene            5322..6011
                     /locus_tag="PK502_09180"
     CDS             5322..6011
                     /locus_tag="PK502_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01028.1"
                     /translation="MVKRIVQIFFLMGMALVGISQECTTMWPYIYPEFKEGTLLMVDG
                     SKLTAQFNVHVQEGRLHYLVKGIIKEARSGDILLANIGDDIYMNVDGSVMKVVGSEER
                     GFVATLILADFDKLLNSTQGAYGASLNSSAKMKLSSIEVGGKTIVDHMLLKQRKEEGE
                     TLPLMYEYFIVTRGKVFPATKKSIMSQLDDKESAEFKKFLKVNKIKWNDPQSLLKLLD
                     FLTVNNLILSI"
BASE COUNT         1749 a         1195 c         1372 g         1815 t
ORIGIN      
        1 aggtccttcg catatgacca acccaattgg atatgccgat tgtgctacgc atagcgattt
       61 acttgcacta tatacctcaa ctgacgttag aaaaaccctt tttagaacag acccggatgg
      121 cgtttcaggt tcgttttgga ctactaagta taaaggtaag ggcgatggtg atgctttggg
      181 aacgcccgat gctaacaacg ttattgttct tcgcctttcg gaaatgtacc ttaatagagc
      241 cgaggcgttg gttaacggtg caactatccc tggtgcaact gcactaagcg acataaatgc
      301 aattgttact aaaagaggat tagcgccata ttcatcagta ggtgccgatg ttgtttttct
      361 tgaacgtcgg ttagaactcg cttttgaggg ccacttatgg tttgattata tcagaacaaa
      421 taggccatct actcgttccg ataaacctct ggctgctgat gattacagat ggttgatgcc
      481 tatccctcaa agagagatgg acgttaatga caatctagtt caaaaccctg aatactaata
      541 aataagtgat aaaaatgaaa aggatacgtt atattctatc actcgcagtt ctggcagttc
      601 ttgcttcgtg taacccaaat gaaatgccgg aatttaacga caatgacgct tttgtggctt
      661 ttgacaatgc atccatgtct gttgaagaaa atgcccaaac gttaagtata cctgttaccc
      721 ttgcgtctat taagggcata tctgcctccg taacatatac gttacttgat ggtggaacag
      781 cagttgaaga cagtaacttt acctttgctg atgccaacaa aaccttaaca tttgatgcca
      841 ccaaccgtac acagtatatt gtggttaaca ttattaataa gcctggtgtg ttcaccggcg
      901 accttaggtt taagcttcag ctttccgacg acggaaccat caagcctaat gccgaaaacg
      961 tatgcactat aactattaca gatctggacc atccactagc ggctatttta ggcacttgga
     1021 cagctaccgg gactagctac tttaacgggt ctgaatcttg ggatatggag cttacaaaag
     1081 atccttccga tgttacggtt gtttggttct acccatttgt caatggaggt tctgggtata
     1141 agatttatgg cgtggtaaac gatgccaaaa ccgagataag agtacccgtt ggacaggaaa
     1201 tagcacctcc aggcagctat ggttttattg agcttaaggg attttatggc ccatcgggag
     1261 aggaagaaat tcctgatggt ggctacatca ccataactat tgagcccaat aagctgtcta
     1321 ttaaggatga aattggctct tatgtgtggg gagatgccgg tcaaacatca ggactaggtt
     1381 ggtacaacat tttcaaagct gatgttgtgc taacaaggta gctcagaacc attaacttaa
     1441 tgaaaaaaat tatcaactat gaagttaaat atgattaaac aaatagctgc gggactagga
     1501 gtattatcat ttgcagtagt cctgtacagt tgtgaggcag attgggagaa ggattacaaa
     1561 gccgtaaccc ttaaagcccc agccactgcc accgtttccg taacatctat agccgactcc
     1621 acagccttgc ttaactactc gctctctgtt accggtaggc tttatgttgc cgttgttccc
     1681 ggtaccgatg aaactcctgc tcctgatgca caggctatac ttaagcaaac agttggaggc
     1741 gctgtttttt caaaacagat tatccttaat gatgctggtg cattaagctc ctcggttatt
     1801 gtttcggggc tggtgcagaa tacctcctac aaggtatttg ctttaccggt gaacgatgat
     1861 ggcgttcttg gtgaaatagt taccacagaa gccttttcaa caagcgacca tcatgccccg
     1921 gttctagatt tagatagtgg tgttaaccct gccattaccg gcaacgcttc aattgacctt
     1981 aactttaaac ctgttctcac ctttgacgag cccgtggtgc ttgcggaaac ctttagcatc
     2041 aaaatgggtt accgtaatgt ggttactgca gaaattgttt gggttgatgt gcctaaggac
     2101 agcatcacca tttcgggtag taaagttacc attgcccagc cggcaaccgc catgaacggg
     2161 cagtacgttt ttttatccat tgggaatggc tctgttaagg atagagctgg taacagctac
     2221 gagggggtta ccagcggtat tgttggaggt tacctggagg gtatctactg gcgcattaag
     2281 ttttctccca ccgctaccca gcaaattcta cccgccgata gcgtgaccag cgatgtgagc
     2341 atgaagatca ccctcgatta ccccataaag atgagattcc caacttcagg agagggtggt
     2401 tacgtgcaag gcaatgtggt tgtaaggtac tatacttcaa caactaccct tgatgtgcag
     2461 gttccacagg ctaatgtagc cgttgtgcaa gataccctgg tgcagataac ccttcccaga
     2521 acacccgttt acggcgaaac ggttaccttg aagatggccg agggtgccat gcgtaacgct
     2581 tacggaaacc cctccaaggc tattgccttt ggcgataagg agtggtttat ctcgtatgga
     2641 tacacaagga atatggttat tgggaactac gtgatcggga acttggtttc cttttctggc
     2701 cctattaccg atgagtacaa cgttattact tcagctcatc caaccgatcc taatttggtt
     2761 gtcataagcg gtttattagg ttcagaagat gatattgtgg ccgagttcaa cggcgacttt
     2821 gcatcgttca acattccaac ctatgacgga atggggcaaa tgatatcatt ccctgatgga
     2881 catccgttgg ctgattacat tttagaggtt tggaatggat attcaagcga tggcgttgct
     2941 actggatttg tcaatgctga tggaaccatt gatatggaag gactaggttt ttatgcgtac
     3001 gacgcagtta cgctagaggc gttgggctgg tatgaccttt tcagccaggc aacttggacc
     3061 cctgctggta agagtatcgt tcaacaaaac tatgttctta agctacaaaa tcataagagc
     3121 aaatttgttc gttaacgcaa ttttgcacgt tacttaatgg ggaggcgatt cttaaacaga
     3181 atcgcctctc ttgatatgtc gggcatacat taatccagca gggtaaaaag tttttgtaca
     3241 cgttgagcaa ttcaaattca acagttttaa aacctgttgg tcctgattag gttggtgatt
     3301 ttttaactca tttacaccga ttttttagga gagacaactt gtgcgccagg ttttaacatg
     3361 aacagatcag catttgtata aagctaatct taatttttcc tgtaaactaa ttctatatga
     3421 aaaattaatt tttcaacaaa aatgttactt ttgctttcaa tttaagaatg ttgtaccgat
     3481 caaagcgtgt ccttctcatg atatattgtc ttgatatagt aactatatgc taacgattat
     3541 tttagtatct taaattttgg ttaaaggtaa ttttgataat aatttaattt ataatcagcc
     3601 tgaaatgaag cgtatcgcat tactagttct tgtatttttt gttcatatca acttatttgc
     3661 tgtagaggta cctcaacgag tagccaagga gcatgccgat gggtttatgt acaaaaaatt
     3721 agataagtca atcaccgaat catcaatttt cccatttcag aagggagaaa atagaaccta
     3781 tacctacctt gtaagctaca aaccggaggg ttgggcaatt gttgcagccg acgataggat
     3841 tccggccata attgcgtact cgcttaaggg tagctttgac accgttaaaa ttaaggaagt
     3901 tccattttac ttctggtttc agcattacgc caaccagata aaggaggttg tggagggaaa
     3961 agaaaccaaa agacatgctt catgggataa ccaccccttt gaggcacaaa aaagcacttc
     4021 acccgttgag ccgcttatag gggttgagtg gaaccagagt tcagggtgga acgcatactg
     4081 tccggctgat agcgagggac ctggtggtca tgcctacgcg ggttgcgtgg ctgtagccat
     4141 gggtcaggca ttgtcggttt accagcaccc tgaagtggga tatggacaga actactatgt
     4201 agatgatacc tacgggagcc ttggtgctgt ttttggcgat acgcagtata agtgggactc
     4261 catcatgggt ttagatcaac caaataagta tgtggctctt cttctctacc atcttggggt
     4321 atcggttgat atgaactatg gcgctgatgg ctcaggagcc ttttccagca tggtacctgg
     4381 cgctttaaaa acgtttttcg actacaccaa cagcactacc atattaaaac gcagcaactt
     4441 tacatccgcc caatgggatt ctatcctagt aagcgagctt gcgaatggac gacctattta
     4501 ctactccggc gatcccggcg atggcacagc tggtcatgca tttaatattg atggagttga
     4561 tgaccagaat cggtaccatt tgaactgggg ctggagcggt gcatacaacg gttactacct
     4621 gattacctcc ttaactccgg gttcaaataa ttttagcagc aaccaggagg ctgtagtgaa
     4681 tattatgcca cgcaatcata accctcagga tatattacta tccaacaaca gcattatgga
     4741 gggattacct gcatatagtc tggttggtaa agttaatgtg ctggatgaaa cacctggcga
     4801 ttctcatacc ctggaggtaa ttgcccctcc tactattgat ggcgagcctg ggtttgtccc
     4861 ctttgctatt ttaggagata gcttattgac aactgaaatt ttaaattttc aaacgagaaa
     4921 ccgctacgaa attattatta aggctaccga caaggctggt cattcgttgg aaaaatcatt
     4981 ttttattaac gtgattaaaa atacaggtgt tagcgaccta actttaagtg gcgaaatgat
     5041 tattacacct acaaatgatg gcaacttaaa agtgtggctt aacaactcag ctataggtag
     5101 ggcaaccatg aatttatacg acattaacgg taaactcata ttttctacta cgattagtaa
     5161 ggaattacaa gtgctggata ccaccattcc actaggtaac actctaataa aaggacttta
     5221 tgttgcagtt ttgaattttg gtaatgaaaa aatttcctat aaatttgtaa agtaggctaa
     5281 gggtcaaggt atgaaattta aaattttaaa ctcgttacag catggttaag cgtattgtac
     5341 aaatattttt tttgatgggt atggcgttgg taggtatttc gcaggaatgt acaaccatgt
     5401 ggccatacat ctatcctgaa tttaaagagg gaactttact tatggttgat ggtagtaagc
     5461 taacagccca gttcaacgtg cacgttcagg agggtcgttt acactatctg gttaaaggga
     5521 ttataaaaga agctaggagc ggggatattt tgctggccaa tattggagat gatatttaca
     5581 tgaatgttga cggcagtgtt atgaaggtgg ttgggagcga ggaacgggga tttgtggcaa
     5641 ccttaatact tgctgatttt gataagcttt taaattctac ccagggtgca tatggtgctt
     5701 ccttaaactc ttctgctaaa atgaagcttt cttccataga ggtgggcggt aaaactattg
     5761 ttgatcacat gctgctaaag cagagaaaag aggaaggtga aaccttgcca ttaatgtatg
     5821 aatactttat tgttacaagg gggaaagttt ttcctgctac aaaaaaaagc attatgtcgc
     5881 aactggacga caaagaatcg gctgagttta agaaattttt gaaggttaac aaaattaaat
     5941 ggaatgatcc acaaagtctt ttaaaactat tagatttttt aaccgttaac aatttaatat
     6001 taagcattta agggttaagg gtttctttaa ccctttttta ttttgtgact ttttgaatgc
     6061 atcagtgtta tatataggta gtatcccaaa aagtgtgtaa agcttgcttt cgattaaaca
     6121 aagatagttc a
//
LOCUS       DAOPHX010000137         6014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_17252_length_6014_cov_6.038094, whole genome shotgun sequence.
ACCESSION   DAOPHX010000137 DAOPHX010000000
VERSION     DAOPHX010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17252_length_6014_cov_6.038094"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1582
                     /locus_tag="PK502_09185"
     CDS             <1..1582
                     /locus_tag="PK502_09185"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPH01029.1"
                     /translation="PDFGQVRSDDRILNLSPFEVKYSEARQFFTEGTELFNKGGIFYS
                     RRVGSTPVDYDKAYESLDTNEVVSDNPSSTKLINASKLSGRTSGGLGIGVFNAVSRKT
                     YAEITDTATGQHRKFLTQGLTNYNMLVFDQSLAHNSYASIVNTNVFRPDGDYTANVTA
                     TDFTIRDKKNKFAVAGVGAFSQIRNDSTSNGYKSSVTLMKTSGNFQGDVWVNVESKHF
                     NPNDLGYLQSPNELSSGVELEYIIFKPFWRLNQLQTSLEYVNQMLYSPRNFVARSISG
                     SIHFITRRYLFGQFYTEVVPQSRYDYYEPRTEGRKLKLPRSIYFNWYGSPDYRKTLAL
                     DHQLEYWKADGYNQYGFSYTIAPRIRFSDKLLLVNSWSQSFDYNAIGYFGGSGSNITM
                     GKRDVVTLTNTLSIQFTFSSKSFINLRARHYIRHYKYDSFYTLNLDGTLTPIASPEYS
                     NKNYNLFNVDFYYQWIFAPGSELVFAWKNMEEEMGTIPINSYFDNVDRVFGSPQYNSF
                     SIKILYYIDYQMIAWLAR"
     gene            1611..2657
                     /locus_tag="PK502_09190"
     CDS             1611..2657
                     /locus_tag="PK502_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HPH01030.1"
                     /translation="MINKHGFASDNNSGVHPAILQAIMEANQGHTLAYGEDMYTEKAK
                     ALFRELLGTDVEVFFVFNGTAANVLGLQAATQRHNAVICAETAHINVDECGAPERITG
                     CKLLTVKTPDGKLTPSLVSTQMHGFGFQHHSQPKVISISQASELGTVYTPEEVKTLSD
                     FAHSHGLYLHMDGTRITNAAVSQSCSLKEISGDAGVDVLSFGGTKNGLMYGEAVVFFN
                     SSIAKDFMYTRKQDLQLASKMRFISAQFIAYLSNNQWATTARHSNQMAQLLADKLRRF
                     SAVKISQKVQVNEVLAIIPPEIIPELQKEYFFYIWDEATSEVRLMTSWDTTEEDIDRF
                     TEILEKLLKKFNML"
     gene            2832..4163
                     /locus_tag="PK502_09195"
     CDS             2832..4163
                     /locus_tag="PK502_09195"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPH01031.1"
                     /translation="MHKRVKFIAAAVFILLGAIFLAFRYDKGVFSKGLTYSGLKYPTV
                     NTLKITNALTSSSEYEWLNNAFNSFIAKWELTGASVAIAKDGRLIYARGFGYASKEEN
                     IPMEPYNLLRVASVSKLITATAVMKLVEENKIQLDSKVFGPNGILNDNIFLNYKDKRV
                     EDITVKNLLNHSAGWSTRWGDHMFINEVVARELHKELPVSLEDIVVFALSKKLHFAPG
                     SHSSYSNLGYAILELVIEKITHTDYETFVQRSIFRPLSIYDARIAQNWDSLRYPNEAR
                     YYEVNEAERVMAFDGSKEGVLKCRGGDDVHTLGAAGGWVISPISLTRFLLAIDGSSSF
                     PDILSKESVAVMTQTSNDKFHPIGWRWVMPGGKLWRSGSLPGTTALAVSSSNGYTYVF
                     ISNTSPWKGARFPYIVDRFFDKLLPGIEGKLPGRNLFFPETSTMGGVASLN"
     gene            complement(4150..4752)
                     /locus_tag="PK502_09200"
     CDS             complement(4150..4752)
                     /locus_tag="PK502_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HPH01032.1"
                     /translation="MPISTKQIPIFPLEVFLLPGEELPIRIFEPRYQELVNDLDKTGE
                     SFGIPFVLNEKIMAYGSEVELSNIVARNSLGQMVVIVKGVRNFHLLQYSEAPTSKPYG
                     SGAIEYIDDDFRSTNPELIVLAKKLKLEISENLGILVKDEAISLASVAKGLMMTSEDK
                     YTFYSLRSKTKMENFLIKRLRFIEQIQKQEKLLANNFSLN"
     gene            complement(4819..5304)
                     /gene="msrB"
                     /locus_tag="PK502_09205"
     CDS             complement(4819..5304)
                     /gene="msrB"
                     /locus_tag="PK502_09205"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HPH01033.1"
                     /translation="MVQRIIITIAFFFTIFSTCLGQSKQVEADNKGDQMNEVKQNLTG
                     LQRYVTQQKGTETPFTGEYWNHFEKGVYVCVCCGAELFESETKFHSSCGWPSFFGSKF
                     EENISFNEDSSHGMVRTEVVCKKCGAHLGHIFNDGPKPIGLRYCINSASLKFIPDKVE
                     Q"
     gene            5592..>6014
                     /locus_tag="PK502_09210"
     CDS             5592..>6014
                     /locus_tag="PK502_09210"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HPH01034.1"
                     /translation="MKKYLFILVGVAISVFASAQNTDSRDITPFDKLKVIGEVKVYLQ
                     RDSVEHISVKTYDVPTSDIHTEVKGKTLEVALKANIYKSVKADIYVYYRELRDISLSA
                     AASISLNDTLTGDKVVINANTSSEFDGGLNVETADIVVG"
BASE COUNT         1650 a         1284 c         1343 g         1737 t
ORIGIN      
        1 acccgatttt gggcaggttc gctccgacga tagaatactc aacctttctc cctttgaggt
       61 gaagtacagc gaggccaggc agttcttcac cgaaggtaca gaactattca ataagggggg
      121 aattttttac tcccgaaggg ttggcagcac tccggttgat tacgataagg cttacgaaag
      181 cctcgatacc aacgaggttg taagcgataa tccatccagc accaagctga ttaatgccag
      241 caaactttcc gggcgaacat ccggaggatt gggtattggc gttttcaatg cagtatcccg
      301 taaaacatac gccgaaatca ccgacacagc taccgggcag cacaggaagt ttttaaccca
      361 agggcttacc aactacaaca tgctggtttt tgatcagtcg ctagcccata actcctacgc
      421 aagtatagtc aataccaacg ttttcagacc cgatggcgac tacactgcta atgttaccgc
      481 aaccgacttt accatcaggg ataagaaaaa taaatttgca gtggcgggag taggcgcttt
      541 tagccaaatt agaaatgatt cgacttcaaa tggctataaa tcgtcggtta cgctgatgaa
      601 aacaagcggt aactttcagg gagatgtttg ggtgaacgtt gaaagtaaac actttaaccc
      661 caacgatttg ggatacctgc aaagtccaaa cgagctttcg agcggtgtgg agctggagta
      721 cattattttt aaaccctttt ggcgattaaa ccagctccaa acatccctgg aatacgtaaa
      781 ccagatgcta tactcaccca gaaattttgt ggccaggtct atttcgggta gtattcattt
      841 tattacccga cggtatttgt ttggtcaatt ctacaccgaa gttgttcccc aatccaggta
      901 cgactactat gagcctagga ccgagggccg aaagctcaag ctgccgcgta gtatatactt
      961 taactggtat ggttcgccag attaccggaa aactcttgca ctcgaccacc agctggagta
     1021 ctggaaagcc gatggctaca accaatacgg attttcctac accattgctc cccggattcg
     1081 ctttagcgat aagctgctgc tggtaaactc atggtcgcaa tcgttcgact ataatgccat
     1141 tggatacttt gggggtagcg gcagcaacat tacaatgggt aaacgagatg tggttaccct
     1201 taccaacacg cttagcattc agtttacctt cagctcaaaa tcttttatca accttagggc
     1261 aaggcactac atacgccact acaagtatga tagcttctac accctaaatt tggatggaac
     1321 gcttacacca attgccagcc ctgaatactc taacaaaaac tacaaccttt tcaatgttga
     1381 tttttactac cagtggatat ttgctcccgg aagcgagttg gtttttgcat ggaaaaacat
     1441 ggaggaagaa atgggcacaa ttcctattaa tagctatttt gacaatgtgg acagggtttt
     1501 tggaagtcca cagtacaaca gcttctcaat caaaatttta tactacattg attaccagat
     1561 gattgcttgg ttagccagat agtttttaac aaccttttaa gtttacatat atgataaaca
     1621 agcatggatt tgccagcgac aacaattcag gtgttcatcc agccattctg caggctatta
     1681 tggaggcaaa ccaggggcat accctggctt atggcgagga tatgtacacc gaaaaggcca
     1741 aagcgctctt tagggagttg cttggaaccg atgttgaggt attttttgtt ttcaacggca
     1801 ccgctgcaaa cgtgttgggt ttacaagctg caactcaacg ccataatgca gtaatttgcg
     1861 ccgaaacagc ccacattaac gttgatgagt gtggtgcacc cgagcgaatt accgggtgca
     1921 agctgctcac tgttaaaact cccgatggaa agctaacacc tagtttggta agcacccaga
     1981 tgcatggctt cgggtttcaa caccattctc agcctaaagt aatttctatt tctcaggcca
     2041 gtgagctggg aacggtttat acacccgaag aagtgaagac actttccgat tttgcccata
     2101 gccatgggct ttacctgcac atggatggaa cccggattac caatgctgcc gtttcgcaaa
     2161 gctgcagttt aaaagagatt tcaggggatg ccggtgttga tgtgctttcg tttggcggta
     2221 caaaaaatgg actgatgtat ggcgaggcag ttgtgttttt caactcctcc attgctaagg
     2281 attttatgta cacccgaaag caggatttac agcttgcttc taaaatgcgg ttcatttccg
     2341 cccagttcat agcctacctg agcaataacc aatgggctac tactgccagg cattccaacc
     2401 aaatggccca gctgttggct gataagctcc ggaggtttag cgccgttaag atctcgcaaa
     2461 aagtgcaggt aaacgaagtg cttgccatca ttcctcctga aattattcca gaactgcaaa
     2521 aggagtactt cttctacatt tgggatgaag ctacttcgga agttcgcctg atgacctcat
     2581 gggataccac cgaggaggat attgataggt ttaccgaaat tcttgagaag cttctaaaga
     2641 aattcaacat gctctagttc ggcactacaa ataggttaga accagggaat tgcctttttc
     2701 aaggttttgc catttgcgaa tggttaattt tccatgctta tttatctggt cttcgtgagt
     2761 gggtgtaaat ttttttgtca tttactctaa tcatagctac ttttgccgct gggtaacttc
     2821 attgaaagct aatgcacaaa agggtaaaat ttattgctgc agctgtattc attcttttgg
     2881 gagcaatttt cctggcgttt cgttatgata agggcgtatt ctctaagggt cttacctatt
     2941 ctggtttaaa atacccaacg gttaataccc ttaaaattac caatgcttta accagtagca
     3001 gcgagtatga atggctgaac aacgctttca acagcttcat tgccaaatgg gagttaaccg
     3061 gtgccagcgt ggctatagcc aaggatggac ggctaattta tgcacggggc tttggatacg
     3121 cctcaaagga ggagaatatc ccaatggagc catacaacct tctaagagtg gcaagtgttt
     3181 caaaactaat tacggctact gctgtgatga agcttgttga ggagaataag attcagctcg
     3241 attccaaagt ttttggaccc aatggaattc ttaacgacaa catttttttg aactacaagg
     3301 ataaaagggt agaggacatt accgttaaaa atctattaaa tcattctgcg ggatggtcaa
     3361 cccggtgggg cgatcacatg tttatcaacg aggtggttgc ccgtgagctg cacaaggagt
     3421 tgcccgtttc attggaggat atcgtagtat ttgccctttc caaaaaatta cattttgcac
     3481 ccggttccca cagctcgtat agcaacctgg gctacgcaat tctggagctt gttattgaaa
     3541 aaattaccca tacggattac gagacttttg tacagcgtag cattttccgt cccttaagta
     3601 tttatgatgc ccgcattgcc cagaactggg acagcttacg ctatccaaac gaagctcgct
     3661 actacgaggt taacgaggca gaacgcgtta tggcttttga tggttcaaag gaaggagttc
     3721 tgaaatgtag gggtggcgat gacgttcata ctcttggggc cgccggtgga tgggttattt
     3781 cacccatcag ccttaccaga tttctactgg ctattgatgg gagcagcagc ttccctgata
     3841 ttctatcgaa agagtcagtt gccgttatga cccaaacaag caacgataag tttcatccta
     3901 ttggatggag gtgggttatg cccggcggaa aactttggcg ttcgggttct ttacccggaa
     3961 caactgcact ggcagtttcg agtagcaacg gttacaccta tgtttttatt tcgaatacca
     4021 gcccgtggaa gggagctaga tttccctata ttgttgacag gttttttgac aagctgttgc
     4081 cgggcatcga gggaaaactt ccgggtagaa atctcttttt tcccgaaacc agcacaatgg
     4141 gcggtgtggc tagtttaaac tgaaattatt tgccaacagc ttttcctgct tttgaatttg
     4201 ctcaataaac ctcaaccttt taatcaaaaa gttttccatt tttgtttttg agcgaagcga
     4261 gtaaaaggtg tatttatctt ctgaggtcat catcagccct tttgcaacgc tcgccaggct
     4321 aattgcctca tctttaacta ggattccaag gttttcacta atttcaagtt tcagcttctt
     4381 tgccagcacg attagctccg ggttggtgga gcggaaatca tcatcaatat actcaattgc
     4441 cccgctgccg tatggtttac ttgtaggcgc ctcgctatac tgaagtaagt ggaagtttct
     4501 aaccccctta actattacga ccatttgccc taggctgttt cgggccacaa tgtttgagag
     4561 ttcgacctcg cttccgtagg ccattatctt ttcgtttaaa acgaagggta ttccaaaact
     4621 ctcaccggtc ttgtccaggt catttaccag ctcctggtag cggggttcaa aaattctgat
     4681 tgggagttcc tctccaggta gcaggaaaac ttccaggggg aaaattggta tttgctttgt
     4741 tgaaattggc atatggttat ttgtttagct aaggttttga ttcctggtag cctaattatc
     4801 ggctatacta tgaaataatt attgttcaac tttatcggga ataaatttaa gcgatgcgga
     4861 gttaatgcag taccgcaagc cgatgggctt tgggccatcg ttgaatatgt gtcctaaatg
     4921 ggcaccacac ttcttacata ccacctcagt cctaaccatg ccatggctgg agtcctcgtt
     4981 gaagcttatg ttttcttcaa acttactgcc aaaaaagctg ggccacccac agctggagtg
     5041 aaacttggtt tccgactcaa aaagttcggc accgcagcaa acgcaaacat atactccctt
     5101 ttcaaaatga ttccagtact cacctgtaaa aggggtttca gtgcctttct gttgggtaac
     5161 gtaacgctgc aacccggtta ggttttgttt tacttcattc atctggtcac ccttattgtc
     5221 tgcttcaacc tgtttcgact gtcctaaaca ggttgaaaaa atggtgaaaa aaaaagctat
     5281 ggtaattata atccgttgaa ccatgatgtt gtagctacta cttattacga aaacgaatat
     5341 ttttctgttt tgttcactct agtgatttac tttaaaacgg caaggcgaaa atgtaatggt
     5401 gaaaatagct gcatgttggt tattttatgt taaagtttgc atttttacaa cttagcatgg
     5461 gtacatttgc atagaagttg tgaaaggtta taacggcaaa cttctgtatc ttaagtaggt
     5521 ttcgattgaa aaatattggc aattttacca tgtattattt cttggactaa attcataaaa
     5581 gcttaatacc tatgaagaag tatcttttta ttctagttgg cgtggcaatt tcggtatttg
     5641 ccagtgcgca gaataccgac agcagggaca taaccccttt cgataagctt aaggtgatag
     5701 gagaggtaaa ggtttacctg caacgagata gcgtagagca catcagcgtt aaaacatacg
     5761 acgtgcctac ctcggatata catacagagg ttaagggaaa aactttggag gtggctctca
     5821 aggccaacat ttacaagagt gttaaggctg atatttacgt gtactacagg gaattaaggg
     5881 atatttcact ttcagcggct gccagtataa gcctgaatga tactcttaca ggcgataagg
     5941 ttgtcataaa tgccaacacc agctctgagt ttgacggtgg tttgaatgtt gagacagccg
     6001 atattgttgt gggg
//
LOCUS       DAOPHX010000138         5883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_17810_length_5883_cov_6.377660, whole genome shotgun sequence.
ACCESSION   DAOPHX010000138 DAOPHX010000000
VERSION     DAOPHX010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17810_length_5883_cov_6.377660"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PK502_09215"
     CDS             complement(<1..893)
                     /locus_tag="PK502_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPH01035.1"
                     /translation="MNFKLVSDFQPSGDQPEAIKQLTDGLGRGDKFQTLLGVTGSGKT
                     FTIANVIAQLNRPVLVLSHNKTLAAQLYGEFKSFFPENAVEYFVSYYDYYQPEAYLPV
                     TDTYIEKDLSINDEIEKLRLSATSSLLSGRRDVIVVSSVSCLYGIGNPQDFYNGTVRV
                     KVGQHLGRNQLLRQLVDGLYARNEVEFKRGTFRVRGDSVDIYLAYGDKGIRVIFWGDD
                     IEALEMFDPVSGVSIETMEEAVIFPANIFIAGKSRINQAIAQIQDDMMRQVEHFRSIG
                     HMVEAKRIQQRVEYDLEMIKE"
     gene            complement(1259..1693)
                     /locus_tag="PK502_09220"
     CDS             complement(1259..1693)
                     /locus_tag="PK502_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPH01036.1"
                     /translation="MTTLEQYNWSNRLILIVDDDRASSMLLELIISKTGAKMIFVSNG
                     RCAVDTFLSTHGIDLILMDIKMEGLNGLDATRLIRERDPHVPIIAQTACVIAGDKEKC
                     LQAGCNDYIPKPININELMETMNKYLSHTNPIVKVNDLFSEN"
     gene            complement(1857..2600)
                     /locus_tag="PK502_09225"
     CDS             complement(1857..2600)
                     /locus_tag="PK502_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPH01037.1"
                     /translation="MGKTITAVVVDDEANSRDILVQMLSFDKDIQVLGASENIDQAMQ
                     AVREHNPDILFLDIEMPGGSGFELLERLRKTPYNPAVIFITAYNQFAIKAIKYAAFDY
                     LLKPIDLDDLVDTLDRYKVNIDRGTMKAKADKLLSCLPEFRKLKFSMRRGIIFIEPEE
                     ITWCEACGSYTILHFHSRKDEVVSVSLKDVESQMEGLPFLRVSRSAIINLKYLAGIDR
                     RNKQCIVQKGDKTYRVPGTNSILKILEAR"
     gene            3245..3802
                     /locus_tag="PK502_09230"
     CDS             3245..3802
                     /locus_tag="PK502_09230"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPH01038.1"
                     /translation="MSVFTLILLALGLSFDTLAVSISSGIARKQIVFLEAFRLAAVLG
                     FFQAAMPILGWLGGISIKKYIEPVDHWVALALLVIIGVKMIIESFKDDGVKTFNPLEP
                     RVMVGMALATSIDALAVGVSFAITQVNLILAFFVFGSITFLVAMLGMLFGKKIGNHIG
                     HRMEIIGGLILIGIGVKIVLEHLNG"
     gene            complement(3805..4500)
                     /locus_tag="PK502_09235"
     CDS             complement(3805..4500)
                     /locus_tag="PK502_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPH01039.1"
                     /translation="MSYTVLKYLVEKNRQPNNTQKEPGPVVTISRQMGCPGNRVTNLL
                     VQELNQRYCLKGESQWNGLAKEKIEQIAAERLGLPKEEIDYVFEAKRKGAMEEILHSM
                     STKYYKSDQRIQNTVKGVIRMEAEKGRVVILGRGGVAITRDIPRSLHIQVEAPFEWRV
                     LRVEEMYHFDNKSAQLYVNEIDKKREAMRVYFGGKGTDYTRFDLTFNAMTLSVKEIVD
                     IIIYTMEVRKLID"
     gene            complement(5169..5441)
                     /locus_tag="PK502_09240"
     CDS             complement(5169..5441)
                     /locus_tag="PK502_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HPH01040.1"
                     /translation="MVAQIIGLLAAILSTLNQFPQAYKVMKTKDTQSISLYMYCIVEV
                     AIALWLVYGIMLRDFPLILANSLSLIPITYIFVVKLLHVISGKESI"
     gene            complement(5459..>5883)
                     /locus_tag="PK502_09245"
     CDS             complement(5459..>5883)
                     /locus_tag="PK502_09245"
                     /inference="COORDINATES: protein motif:HMM:NF040269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01041.1"
                     /translation="EYRVITVGFYSLVNLLNFDSSKLLNVAEWHNVDEVESLIFDHKE
                     ILGEALKKLKEDVTMHPIVFELLPKKFTLTQLQQIYEVILGTRFDKRNFRRKIANLKY
                     VVSCNEKQYGVGHKPPSLFRFDPKIYSKIHQKNLDATA"
BASE COUNT         1521 a         1311 c         1329 g         1722 t
ORIGIN      
        1 aactccttta tcatttccag gtcgtactcc acccgttgct gaatcctttt ggcctcaacc
       61 atgtggccta tgcttctgaa gtgctccacc tgcctcatca tatcgtcctg aatttgggca
      121 atggcctggt ttattcggct tttacctgca ataaatatat tggcagggaa aattaccgcc
      181 tcctccatag tctcgatgga aaccccgcta accggatcga acatttcgag cgcttcgatg
      241 tcgtcgcccc agaaaataac cctaatgccc ttgtctccgt aggcaaggta tatgtccacg
      301 ctatcgcccc tgacccggaa ggtgcccctt ttaaactcca cctcgtttcg ggcgtaaagc
      361 ccatcaacca gctgtcgtag tagctggtta cggccaaggt gctgaccaac tttaacccga
      421 acggttccgt tgtagaagtc ctgtggattt ccaattccgt agaggcatga aacggaggaa
      481 accactatta catccctacg gcctgatagg agggaagagg tggcgcttag ccgcagcttt
      541 tcaatctcat cgtttatgga taggtccttc tcaatgtagg tgtcggtaac aggtaggtag
      601 gcctcaggct ggtagtagtc atagtacgaa acaaaatact caacagcatt ttcggggaaa
      661 aaacttttga actccccgta aagctgagct gccagcgttt tgttgtggct tagcaccagc
      721 acaggcctgt ttagctgggc aataacgttg gcaatggtga acgtttttcc tgaaccggta
      781 actcctaaaa gggtctgaaa cttatcgccc cggcccagcc catcggtcag ctgcttaatg
      841 gcctccggtt gatcgcccga tggttgaaaa tcggaaacaa gcttgaagtt cataccttag
      901 tgtgcgagtt ggtgcatcga ttactgacat ctgcagccag cttatcaaaa acattataaa
      961 tccgaaaaaa gcttccagga tgcctaaaaa gttagtccag attatttttc tgcaaaatta
     1021 gctattttaa cggcttgttg gaagtgctgg gacaggtagc cggttagaac ctgacgggta
     1081 gcctgaatgt gaaaaagttt acaaggtgga tgtagccggt gcctaaaatt gcttacctta
     1141 tttatgctgc tgaattgggc aggcaatatg aatgttgtct tattcggctt acggatcagt
     1201 ataaccagtg agagcataag ccattgctat tgatggaaat gccggtagga agaagctatt
     1261 agttttcaga aaacaggtcg tttaccttga caatcggatt tgtatggcta agatacttat
     1321 tcatggtttc catcagctcg ttaatgttaa ttggcttagg gatgtagtcg ttacaccctg
     1381 cctgaaggca tttctcctta tcgccggcaa tgacgcaagc cgtttgggca ataattggga
     1441 catgcgggtc gcgctctcgg attaaccggg ttgcgtccag cccattaagg ccttccatct
     1501 tgatgtccat gagaattaag tctatgccgt gagtgctgag aaaggtatcc acagcacatc
     1561 tcccgttaga gacaaaaatc atctttgccc ctgttttaga aattataagc tcaagcagca
     1621 ttgagctggc acgatcatca tcaacaataa gtatcaacct gttactccag ttgtactgct
     1681 caagcgtggt catttaggaa gttgtaagta gctttaaatt gaaagcaagt tatagctttt
     1741 tacaggttta tatcaactga aacatgctta aaaattacga aaatggggga ttgccttatg
     1801 aagctaataa ttttggagac taatcctaaa atttggtttt ttaaaaggaa tagcgtttac
     1861 ctggcttcga gaattttcag aatcgagttt gttccgggta cccggtaagt tttatcgccc
     1921 ttttgcacaa tgcattgctt gttacggcgg tctattcccg ccaggtattt taggttgata
     1981 atggccgaac ggcttacccg tagaaatggt aacccctcca tttgcgattc cacgtctttg
     2041 agcgaaacag aaaccacctc gtcctttcgg gaatgaaagt ggaggatggt gtagctgcca
     2101 catgcctcgc accaggtaat ctcctcgggt tcgatgaata taattcccct gcgcatgctg
     2161 aacttcagct tcctgaactc gggaaggcag ctcagcagct tatcggcttt ggccttcatc
     2221 gttcccctgt cgatgttcac tttgtagcga tcgagggtgt caacgagatc gtctaaatct
     2281 attggtttta ggaggtagtc gaaagccgca tactttatag cctttatggc aaactggttg
     2341 taggcagtaa tgaatataac cgcagggtta tagggagtct tacgcaacct ttcgagcagc
     2401 tcgaaccctg aaccacccgg catctcaata tccagaaata gaatatcggg gttgtgctcc
     2461 ctgactgcct gcatggcctg gtcaatattc tcggatgctc ctaacacttg aatatccttg
     2521 tcgaacgaaa gcatctgaac caggatgtcc ctggagttgg cctcgtcatc aactacgaca
     2581 gcagttatag ttttgcccat tatacttagg tatattccaa acttcaaagc cgagtgatcc
     2641 agctcatttt ttacccgtgc aaaattttat tgctttaacc ccgtaagcta cgattttgtt
     2701 atatacactc gtttatattc cgatgaaccc caaagggttt gccataagca aacctccatt
     2761 aaccggcttc gctgaactcg cataaaacgc ttggtagttt attcgctctt gtacaagcca
     2821 tctacatggg cgtttcataa ttatgcaatc ctgctgccac aaaatttttg aggtaagcta
     2881 ccatcaactt caaaagtagc tattatttga taattttctc caacaagcca tcttaaattc
     2941 atcctggcaa ccatgttgct ggcgtaaggg gttgacagcc aagcatgttc accgctgccg
     3001 ggccatacaa accctccaaa tatttttggc tgagcaaccc gagttacagg ggtcttcaat
     3061 tttttagttg cacagcttga cgttaatggc aatatgacta gttcaattta aaaaaatgcg
     3121 aaaagtaagg agttgaataa gaagtatata atgttgatat aaaagcaaaa gcgctcaaat
     3181 caaaataatg ggtttgctgt acggtattgg ttgttatgtt taactttgcc gccaaaagca
     3241 attcatgtca gtattcaccc ttattctcct ggctttgggc ctttcgttcg ataccctggc
     3301 tgtttccatt tccagtggaa ttgcacgtaa gcagatagta tttttggaag ctttccggtt
     3361 ggctgctgtg ctgggattct tccaggcagc catgccgatt ttgggttggc ttggcggaat
     3421 atccataaag aagtacattg aaccggttga ccactgggtg gcgctcgccc ttttggtcat
     3481 tataggcgtt aaaatgatca ttgagagctt taaggatgat ggggtaaaga catttaaccc
     3541 gctagaacca agggtgatgg taggaatggc gttagccacc agcatagatg cgctagccgt
     3601 tggagtaagc tttgcaataa cgcaggtgaa ccttattctg gcatttttcg tctttggctc
     3661 cattaccttc ctggtagcca tgctgggaat gctgtttggg aagaaaattg gcaaccacat
     3721 tgggcaccgt atggagatca taggagggct aatacttata gggatagggg taaagattgt
     3781 gctcgagcac ctgaatggct agcgctaatc aatcagctta cgcacctcca tggtgtagat
     3841 gatgatatca acaatttcct taacggagag ggtcatagcg ttaaacgtta ggtcgaaccg
     3901 ggtgtagtcg gttcctttcc cgccaaagta aacccgcatg gcctcgcgct ttttgtctat
     3961 ttcgttcacg tagagctggg ctgacttatt gtcaaaatgg tacatttcct caactctaag
     4021 cactctccat tcaaagggtg cctcaacctg gatatgtaac gagcggggaa tatcgcgggt
     4081 aatggccact ccacctcttc ccagtatcac cactcgtcct ttctccgcct ccatccggat
     4141 tacacctttt accgtgtttt gaatccgctg gtcgctcttg tagtatttag ttgacatgga
     4201 gtggagtatt tcctccatag ctccttttcg tttggcctca aaaacgtagt cgatttcctc
     4261 ctttggcaaa cctaatcttt cagcagcaat ttgttcaatt ttctctttcg ccagcccgtt
     4321 ccactggctt tctcctttaa ggcagtagcg ctggttaagc tcctgaacca gcaggttggt
     4381 aaccctattg cccggacacc ccatctggcg cgaaatggta accaccggcc ctggttcttt
     4441 ctgggtattg ttgggctgac gattcttttc aaccagatat ttgagtacgg tatagctcat
     4501 gggttaatgt ataaaatgat atttaacatt ggctaaatat aacttttttt tcctattaag
     4561 tcaataaatt ttctaaagaa actgtcatgt ttttgtaaac ataaccaccc acaagcaacc
     4621 gagcgttttt tgctgttccg gctaagccaa aacatgtgtg ttccacttgc tcaacccttt
     4681 tacctataag ttactggcta gggttcacac aaccagatat ataaatattt tatgcggttg
     4741 aattgctgtt taaagcctaa ttgatttcta atagcatttt acccattttt acttcccctg
     4801 taaacttcag ctgatagtta caccacaacc ggaattttgg tttcaggagc cgccatcatg
     4861 gcactgctgt cggtagggtt gagtgaggat tttggccaac atggagacgc aatacagccg
     4921 cttgcctttg aagataattt gagaagaatt taatgcgaaa tgaatgtgcg ggtagccaaa
     4981 atcccttttg gcttgtgtgg ggtgagccag caaggttatg aagcagcagg ttggatgtgt
     5041 cccgctgttc agaatcgcat cccccactgt cattttttag ttgccgcttg agccctaaaa
     5101 aataagggga ataccttgct gattgtcata ataacacatt taaaatggaa taattaacag
     5161 gaagctgcct agatgctctc ctttcctgaa ataacatgga ggagcttcac cacaaaaatg
     5221 tacgtgatag gaatcaggga aagggagttg gccagtataa gcgggaagtc tctaagcatt
     5281 attccgtaaa ccagccaaag cgcaattgcc acctcaacta tgcagtacat atacagggag
     5341 atgctttgcg tgtccttggt tttcatcacc ttgtaggctt ggggaaactg gtttagagtt
     5401 gagaggattg cggccagcag tccgataatt tgggcaacca taattattta tgttgaggtt
     5461 aagcggtagc gtcgagattt ttctggtgta ttttggagta aatttttggg tcaaatctga
     5521 atagtgacgg aggtttatgg cccacgccgt actgtttctc gttgcaggaa accacgtact
     5581 tgaggttggc aattttgcgg cggaaattcc gcttatcgaa acgtgtaccc agaatcactt
     5641 cgtaaatctg ttgaagctgg gtaagggtga atttcttggg aagcagctca aatactatgg
     5701 ggtgcatggt tacatcctcc ttgagttttt ttagggcctc accgagaatt tccttatggt
     5761 cgaaaatcaa gctctccacc tcgtcaacat tatgccattc ggcaacattt agaagtttag
     5821 aagaatcaaa gtttagcagg tttaccagcg agtaaaaccc tacggtaatt accctatact
     5881 ccc
//
LOCUS       DAOPHX010000139         5797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_18169_length_5797_cov_5.176245, whole genome shotgun sequence.
ACCESSION   DAOPHX010000139 DAOPHX010000000
VERSION     DAOPHX010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18169_length_5797_cov_5.176245"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PK502_09250"
     CDS             complement(<1..318)
                     /locus_tag="PK502_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPH01042.1"
                     /translation="MLRLAIIGYGKMGHEVESMAMERGHEVTLRIDRDNTHELTPENL
                     KLVDVAVEFTSPKSAKANVEACLNAGVPVVCGTTGWGNELEAVIERVKVGGGTLFYAS
                     NYSV"
     gene            complement(451..795)
                     /gene="rplT"
                     /locus_tag="PK502_09255"
     CDS             complement(451..795)
                     /gene="rplT"
                     /locus_tag="PK502_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPH01043.1"
                     /translation="MPRSVNAVASRKRRKKILKQTKGNFLARRNVYTVAKNTLEKGLT
                     YAYRDRKKKKIEFRALWIQRINAAVRPYGLTYSEFMGKINKKGIAINRKVLADLAMNN
                     PEAFKSIVDSVK"
     gene            complement(904..1101)
                     /gene="rpmI"
                     /locus_tag="PK502_09260"
     CDS             complement(904..1101)
                     /gene="rpmI"
                     /locus_tag="PK502_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPH01044.1"
                     /translation="MPKIKTHSGAKKRFSLTGTGKIKRKHAYKSHILTKKETKQKRNL
                     THFTLVDKADEKRVKKMLGLR"
     gene            complement(1128..1682)
                     /gene="infC"
                     /locus_tag="PK502_09265"
     CDS             complement(1128..1682)
                     /gene="infC"
                     /locus_tag="PK502_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPH01045.1"
                     /translation="MATPLHSQVKKDVDNKFLVNNEIRNKVVRLVGENIENPGIFSIE
                     EARRLANELELDLVMISDKADPPVCRIIDYQKFLYQQKKKQKEIKANAQKIVVKEIRF
                     GPNTDEHDYNFKLKHAQNFLKEGAKVKAYVFFKGRSILFKEQGEILLLRFAQDLEDLG
                     KVEQLPRLEGKRMIMLISPKGAKK"
     gene            complement(1712..3652)
                     /gene="thrS"
                     /locus_tag="PK502_09270"
     CDS             complement(1712..3652)
                     /gene="thrS"
                     /locus_tag="PK502_09270"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPH01046.1"
                     /translation="MVKITFPDGSIREYPLGVTGIEIARSISERLAAEVLSITVNGEV
                     WDLTRGITTDATVKLHKWDDEEGKHTFWHTSAHLMAQALEALYPGIKLGIGPSIDNGF
                     YYDIDFDSYTISDDDFPKIEQKMAELAHQKLDVVRKDVSKDEALATYKAKGDPYKVEL
                     ISDLQDGTITFYTQGDFTDLCRGPHLPNTGYIKAIKILSIAGAYWRGNEKNKMLTRIY
                     GITFPQKKLLDEYLVMLEEAKKRDHRKLGKEMELFTFSQHVGQGLPLWLPRGAQLRFQ
                     LEMFLRKVQQKYGYEQVITPHIGQKELYEISGHWAKYGKDSFRPITTPQEGEEFLLKP
                     MNCPHHCEIYKSKPRSYKDLPVRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCR
                     PDQLKQEFVKVIDIVLYIFKTLNFKEYTAQVSLRDPKDREKYIGTDENWEKAESAIIE
                     AAAERGLKTTTVVGEAAFYGPKLDFMVNDAIGRKWQLGTIQVDYNLPERFQLEYIGSD
                     DKRYRPVMIHRAPFGSMERFVAVLLEHTGGKLPIWLAPDQVVVLPISEKYSDYAKKVF
                     NYLNNSDIRTLIDDRNEKIGKKIRDNELRHIPYLLIVGEKEEENGTVAVRVQGEGDKG
                     QMSLDDFIMLVRNNIESQLTEI"
     gene            complement(3844..5136)
                     /locus_tag="PK502_09275"
     CDS             complement(3844..5136)
                     /locus_tag="PK502_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPH01047.1"
                     /translation="MFKELVSKNEKMAVIGLGYVGLPIALEFAKKISVVGFDIKPDRV
                     ELMKRGVDPSHELESTSFQNCDITFTSDINDLRKASFYVVAVPTPIDEHNLPDLKPLL
                     AATRTVGQVLKKGDYVVYESTVYPGCTEEDCVPILEELSGLKFIQDFKVGFSPERINP
                     GDKNHTLTTIKKITSGCDPESAEEIAKTYELIIKAGIHRASSIKVAEAAKIIENTQRD
                     INIAFMNELSIIFSRMGINTYEVLEAAGTKWNFLNFSPGLVGGHCIGVDPYYLVYKAK
                     ELGYHPQIISAGRYINDTMGGYVAKQTVKKIIAAGTNPMDARVLVMGVTFKENVSDIR
                     NSKVVDIYRELRSFGVNYIDIIDPYADAHDVKHEYGFDMATAPSGRYDAIILAVGHSQ
                     YAGLKEEWFKRLAAEKCVFVDVKGIFRSKINNLTYWSL"
     gene            complement(5160..>5797)
                     /locus_tag="PK502_09280"
     CDS             complement(5160..>5797)
                     /locus_tag="PK502_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPH01048.1"
                     /translation="PVVLNPWNIDALSEIEHETGRHIYTILQLRLHPAIIEFKKKIDN
                     GPKDKIYDVDLTYITSRGRWYFISWKGDVQKSGGVATNIGVHFFDMLTWIFGDVKSGV
                     VHMSMPDKAAGYLELQKARVRWFLSIDYNDIPDTVKQQGKRTYRSITVGNDELEFSEG
                     FTDLHTTSYRDILSGNGFGLNDARSSIETVYAIRNAVPVGLKGDYHPFCKK"
BASE COUNT         1505 a         1381 c         1202 g         1709 t
ORIGIN      
        1 aacgctatag tttgaggcgt aaaaaagcgt tcccccaccc actttaaccc gctctataac
       61 cgcctccaac tcattacccc aacctgttgt tccacaaacc acaggaactc cggcattcag
      121 acatgcctcc acatttgctt ttgccgactt gggcgaggta aactcaacag caacatccac
      181 caatttcagg ttttcgggag ttagctcatg agtattatcc ctatcaattc ttaaagtaac
      241 ctcatggcct ctctccatcg ccatggattc aacctcatgc cccatcttcc cgtatccgat
      301 tatagccagc ctcaacataa agcctaattt ttctgcaata tagctataat atttgttatc
      361 attgctacag gcaatcgtcg agggaatggg ttaaaataaa ggaggggtgc ttacccctcc
      421 aaaacgttat gctgaatatg ctggcgaaac ctacttaaca gaatcaacaa ttgacttgaa
      481 agcttcgggg ttgttcatgg ccaggtcggc cagaacctta cggttaatcg caataccctt
      541 cttgttgatt ttacccataa attccgagta ggttaaccca taaggcctaa cagcagcgtt
      601 aatacgctga atccacaaag cgcgaaactc aattttcttc ttctttcgat cccgataggc
      661 ataggttaaa cctttctcaa gggtgttctt agcaacggtg taaacgttgc gacgggcaag
      721 aaaattaccc ttggtctgct tcagaatttt tttccttctc tttcttgatg caacagcatt
      781 gactgaacgt ggcatagttt taaaattttt gaatggttag cgttccgcaa cccggacact
      841 taggcgctaa ttcattctgg ttagtaatta aaagtagctg taaataaaag gctaaagtca
      901 tggctacctg agcccaagca tttttttgac ccttttctca tctgccttgt caacaagggt
      961 aaagtgggta aggtttcttt tttgtttggt ttccttcttg gtaaggatat ggctcttgta
     1021 agcatgtttc cttttaattt ttcctgttcc ggtcaaagaa aaccttttct tggcaccgga
     1081 atgagtctta attttcggca ttctaaactg aaattattta ggtttattta cttctttgct
     1141 ccttttggag atattaacat gatcattcgc ttgccttcca acctgggtag ctgctcaacc
     1201 tttccaaggt cttctaaatc ctgggcaaat cgtaggagta atatttcgcc ctgctcctta
     1261 aaaagtattg aacgtccctt aaaaaatacg taggctttca cttttgcccc ttctttaagg
     1321 aaattctggg catgtttcag cttaaagttg tagtcatgct catcggtatt tgggccaaaa
     1381 cgaatttcct taacaacgat tttctgtgca ttagccttga tttccttctg tttctttttt
     1441 tgctggtaga gaaacttctg gtaatcaata atgcggcaca ccggtggatc ggccttatca
     1501 gaaatcatca caaggtctaa ctccagctcg ttagctagcc gcctagcctc ttcgatggaa
     1561 aaaatacccg ggttctcgat attttccccg acaagccgca ctaccttgtt tcttatttca
     1621 ttgtttacca ggaatttatt atctacgtcc tttttaacct gggaatgtaa tggtgttgct
     1681 atagctttgt cctcctattg gttatagtta actatatttc agtaagctga ctttcaatat
     1741 tattcctaac cagcataata aaatcatcga gggacatttg tcctttatcg ccttcacctt
     1801 gaacccgaac ggcaaccgtg ccgttttcct cctccttttc gcccacaata agcaggtagg
     1861 gaatatggcg taactcgttg tcccttatct ttttaccaat tttctcgtta cggtcgtcaa
     1921 tcagggtgcg aatatcggaa ttatttagat aattaaaaac tttttttgcg taatcgctgt
     1981 acttttcgct gatgggcagc actacaacct ggtcgggtgc aagccaaata gggagcttcc
     2041 ctccggtatg ctcaagaagc acagccacaa agcgttccat tgagccaaat ggggcacgat
     2101 gtatcatcac cgggcggtag cgcttgtcgt cggagccaat gtactccagc tgaaagcgct
     2161 caggcaggtt gtagtcaacc tgaatagtcc ccagctgcca ttttcggcca atggcgtcgt
     2221 taaccatgaa atcaagctta ggtccgtaga atgccgcttc gcctacaacg gtggtagttt
     2281 taagtccccg ttcggcagca gcctcaataa tggcgctttc ggctttttcc cagttctcgt
     2341 ccgttccaat gtatttttcc ctatcctttg ggtcacggag tgaaacctgt gcggtgtact
     2401 ccttaaaatt gagggtttta aaaatataca aaacaatatc aatcaccttg acaaactcct
     2461 gcttgagctg gtcggggcga cagaagatat gggcatcgtc ctgagtaaac cccctaacgc
     2521 gtgttaaccc gtgaagctcg ccgctttgct cgtagcggta aaccgtccca aattctgcta
     2581 acctaaccgg taagtcctta taggaacggg gtttagattt ataaatttca cagtgatgtg
     2641 ggcaattcat gggtttaagg agaaactcct ccccctcctg aggggtagta atgggtctaa
     2701 agctatcctt gccgtatttt gcccagtgcc cagaaatctc gtaaagttcc ttttgcccta
     2761 tgtgaggagt tattacctgt tcgtagccgt acttctgctg tacttttcga aggaacatct
     2821 cgagttgaaa gcgaagctgg gcgccacggg gcagccatag gggtaacccc tgaccaacat
     2881 gctgcgaaaa ggtaaatagc tccatttctt tccccagctt acgatggtcg cgctttttgg
     2941 cctcttccag cattaccagg tactcgtcga gcaatttttt ctgtggaaag gttatgccat
     3001 agatacgggt tagcattttg tttttctcgt tcccacgcca gtatgctcct gcaatactta
     3061 aaattttaat agccttaatg tagcctgtgt ttggaaggtg tggcccacgg caaaggtcgg
     3121 tgaaatctcc ctgggtgtag aaggtaatgg tgccgtcctg taggtcgctg ataagctcaa
     3181 ctttgtatgg gtcgccttta gccttgtatg tggctagtgc ctcgtctttt gaaacatcct
     3241 tcctaacaac atcaagtttt tgatgggcca gctcggccat tttctgctca atttttggga
     3301 aatcgtcatc agaaatagtg tagctatcaa aatctatatc gtagtaaaaa ccgttgtcaa
     3361 tgctagggcc aattcccaac ttaatgcccg ggtaaagggc ctcgagtgcc tgcgccataa
     3421 ggtgagcgga tgtgtgccaa aaggtatgct ttccctcttc gtcatcccac ttgtgcagct
     3481 ttacggtggc atcggtagta attccacgag tcaaatccca aacctcacca ttaacagtga
     3541 ttgatagaac ctcggcagcc agtcgttccg aaatgcttcg ggcaatttca atcccggtta
     3601 ccccaagggg atactcccgt atgctaccat caggaaaagt aatctttacc atcattataa
     3661 acttgatata cttgtaataa aagttaaaga ttgcaaagta atactattaa ctccaaattt
     3721 gcaaggcatt cacgaatggg gctggtttat ggcattagcc cgttaaggca aacatggcca
     3781 gattattatg ccatgtttag tacggattag tagaaatacc agccaaacta tccatcaaac
     3841 cacctaaagg ctccagtagg tgaggttgtt aattttgctc ctaaaaatac cctttacatc
     3901 tacaaaaacg cacttttcgg cagcaaggcg cttaaaccat tcctccttga gtccagcata
     3961 ctggctatgc ccaacggcta gaatgattgc gtcatacctg cccgatggag cagttgccat
     4021 gtcaaaacca tactcgtgct ttacgtcatg ggcatcggca tagggatcaa taatatcgat
     4081 atagttaact ccaaacgatc ttaattcacg gtagatgtcg actaccttgg agttccgaat
     4141 atcgcttaca ttttccttga aggtaacacc cataaccagc acccttgcat ccataggatt
     4201 tgttccggca gcaataattt tcttaacggt ttgcttggcc acatatcccc ccatggtatc
     4261 gttgatgtaa cgaccagcgc tgataatttg cggatgatag cccagctcct tggccttgta
     4321 aaccaggtag tatggatcaa ctccaatgca atggcctcca acaagcccgg gtgagaagtt
     4381 aagaaagttc cacttggttc ctgccgcttc gagcacctcg taggtgttta tacccatgcg
     4441 gctgaatatg atggagagct cattcataaa cgcaatgtta atatcgcgtt gggtgttctc
     4501 aataattttt gcagcttcgg ccaccttaat ggagctggca cggtgaatgc ccgctttaat
     4561 aatcaactca taagttttag ctatttcctc ggctgattca ggatcgcagc ccgaggtaat
     4621 tttttttatg gttgtaaggg tgtgattctt atcgcccggg tttattcgct cgggcgaaaa
     4681 tccaacctta aagtcctgaa taaattttaa cccggaaagc tcctcaagaa tggggacaca
     4741 atcttcctcg gtgcaacccg gataaactgt tgactcgtaa accacgtagt cacctttttt
     4801 cagcacctga cctaccgttc gggtagcggc aagtaatggt ttcaaatcgg gaaggttatg
     4861 ctcatcaatg ggtgtgggaa cggccacaac gtaaaatgac gcctttcgta aatcgttaat
     4921 atcagaagta aatgttatgt cgcagttctg aaacgacgta gattcaagct catggcttgg
     4981 gtcaactcca cgcttcatca gctcaaccct gtcgggttta atatcgaatc caactaccga
     5041 aatttttttg gcgaattcca gggcgatcgg aaggccgaca taacctagtc ctataaccgc
     5101 cattttttca ttctttgaaa ctaactcctt gaacatatag tgaatgttgt tatattctat
     5161 tacttcttgc aaaaaggatg ataatcgccc ttgagcccga ccggaacagc gttgcgaatc
     5221 gcataaaccg tttcaattga gctacgggca tcattcaggc caaatccgtt tccagaaaga
     5281 atatcgcggt agctggtggt gtgcaaatcg gtaaaaccct cgctaaactc aagctcatca
     5341 ttccctacgg taatggaccg gtaggttcgt ttcccctgct gcttcactgt gtcgggaata
     5401 tcgttgtaat caatgcttag aaaccaacgg acgcgagctt tttgtaactc aaggtaaccg
     5461 gcagccttat cgggcataga catatgaaca acgccacttt tgacatcccc aaaaatccaa
     5521 gtaagcatat caaaaaagtg cactccaatg ttggtggcaa ctccaccaga tttttgaacg
     5581 tcgcccttcc acgaaatgaa ataccaacgc ccacggctgg tgatgtaggt taagtcaaca
     5641 tcgtaaattt tatccttggg gccattgtca attttctttt taaactcaat aatggccgga
     5701 tgaagcctaa gctgaagaat agtgtagata tgcctgccgg tttcatgctc aatttcacta
     5761 agggcatcaa tattccatgg gttcagaacc acgggtt
//
LOCUS       DAOPHX010000140         5769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_18309_length_5769_cov_5.604130, whole genome shotgun sequence.
ACCESSION   DAOPHX010000140 DAOPHX010000000
VERSION     DAOPHX010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18309_length_5769_cov_5.604130"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PK502_09285"
     CDS             complement(<1..267)
                     /locus_tag="PK502_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase subunit B"
                     /protein_id="HPH01049.1"
                     /translation="MKGNEVKKKIWSEYQKEIADDKFYFVRSCIRQTFFPGSDTVYLK
                     ILRDVLGKDVYEDANHTTCTGIGYHCEVVPFPTIQTVVARNFALM"
     gene            complement(380..1099)
                     /locus_tag="PK502_09290"
     CDS             complement(380..1099)
                     /locus_tag="PK502_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPH01050.1"
                     /translation="MGKLFDRLNSDVRFIEGINGCINCGTCTAICPAAEVYDYEPRSI
                     ANLVQTHDDEVIENLLKSDTIWYCGECMSCKTRCPRNNAPGLIIQALRKLSIETGLFV
                     ESEKGRQQLSLKRMLGNSILETGYCVHFDHTELSMFPELGPVWQWVRDNRETVLKKVG
                     ANYKGSGPGAIRKISQESLDELERIFEVTGGLEWFKKIEEDSSEMADKLSLEIGNETS
                     KESPYFRKVYRHNDPEKHTRK"
     gene            complement(1333..3291)
                     /locus_tag="PK502_09295"
     CDS             complement(1333..3291)
                     /locus_tag="PK502_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPH01051.1"
                     /translation="MKRHLPILFLIILGSLGHSPLYGQLNKAYYYYKSEELISQGRYS
                     ESLPVLNTLIGIDSSLFEGWFLRGVAKYNLGDIRGAITDFDKSLKANPLSAQSYHYKA
                     IALSQVSEYDKALKEISSALELRPNSPDFLFTLGVLQFQTQNYPKAIGTFTRVIQLES
                     SNADAWYNRATAKLLIADTLGAISDYSTTINVNPFHAAAYARRGAVNAELDRNHLALA
                     DLNRAIELDSLKADFYFTRAIINYNFKKYQNSIDDLTKALRLNPNYQIALFNRAIINY
                     QAGNTDPAIDDLTRLMAINSQNVLVPYYRGSIYLEKGEATRSIDDFTKAISLFPDFAN
                     AYLLRSDAKRQLGNLPGAEEDFLKGQALANKFHGKGQAEITSLLDSSGKLNKLISLES
                     DFNLASTLSIESNRIRIASAFMPMIRLHFNQPDKPATNNNLASVYLDSINATLPINYK
                     LIFGFAPSNSTEKLSIESADSTIANFPALCLVKGIFLAENGKFASATNEFKKAFEQNS
                     SNPIYLFSYIMNNVEMAEFIENFNTSTEISGIAMSSSKNTGITSITAYNDAIVELKGL
                     YEVFPKNGLIAYNLGNLSIMTNDYESAISWFSLAVRENPLLSCAWFNKGLSLLITGNK
                     DEGCNNLGKAGELGVEEAYMAIKKFCTR"
     gene            complement(3305..4051)
                     /gene="mtgA"
                     /locus_tag="PK502_09300"
     CDS             complement(3305..4051)
                     /gene="mtgA"
                     /locus_tag="PK502_09300"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HPH01052.1"
                     /translation="MNGKNRQKSQRYQSFRRLVLYAIATFFITTVISVIAFSFINPNL
                     TMLMVAKRVQPLNDGGSNRINHKWVSIDKISPQLVLAVCAAEDNKFMEHNGFDWESIQ
                     KAKEHNKKGKRLRGASTITQQTVKNVFLWPRRSWVRKGFEAYFTFLVEVFWSKERIME
                     VYLNVAELGRGIYGVESASECYYGKPASKISTYEAAMLATVLPNPAKRNPAKPSSYMY
                     RYQRTILRNMYNLGRIDLKGDTSSKSKKKN"
     gene            4486..4899
                     /gene="mce"
                     /locus_tag="PK502_09305"
     CDS             4486..4899
                     /gene="mce"
                     /locus_tag="PK502_09305"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPH01053.1"
                     /translation="MSVTHIEHIGIAVKSLNDAIPYYENLLGVKCYSIEEVKDQKVKT
                     AFFKVGQTKIELLETTDPEGTIGKFIEKKGEGIHHIAFAVKGINEELQRMQENGIVLI
                     DKTPRSGAEGLEIAFLHPKSTFGVLTEFCEDRNQK"
     gene            4921..>5769
                     /locus_tag="PK502_09310"
     CDS             4921..>5769
                     /locus_tag="PK502_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPH01054.1"
                     /translation="MSIQEQKIKELIELREKAKLGGGARRIESQHKKGKYTARERIEM
                     LLDEGSFEEFDMFVTHRCTDFGLDKETYLSDGVITGYGTIDGRLVYVFSQDFTVFGGS
                     LSEMFAAKICKVMDMAMKVGAPVIGINDSGGARIQEGVTSLGGYAEIFERNILASGVV
                     PQISAIFGPCAGGAVYSPALTDFIVMSRNTSYMFVTGPKVVKTVTGETVTSEELGGAG
                     VHSSKSGVAHFVAENEEEGIMLIRKLLSYLPQNNLEEAPIMPCDDPIDRLEDSLNDII
                     PDNPGKP"
BASE COUNT         1493 a         1205 c         1320 g         1751 t
ORIGIN      
        1 catcagggca aaatttcggg caacaacggt ttgaatggtg ggaaaaggaa ccacctcgca
       61 gtggtagcca attccggtgc aggtggtgtg gtttgcatcc tcgtaaacgt ccttacccag
      121 cacatcccgc agtattttga ggtaaactgt atcgctgccc gggaaaaatg tttgacgtat
      181 gcagctgcgg acgaagtaaa acttatcatc tgcaatttcc ttctggtatt cgctccaaat
      241 tttctttttt acctcgttgc ctttcatggt tcctgttatg tttagctcag atattcttta
      301 cgtttgtgaa aatggtagga ggattgctcc cattttttaa aataccgtat agttgtgcag
      361 gcagctagct ttatttaaac tacttacgtg tgtgcttctc aggatcgttg tgtctgtata
      421 cctttcggaa gtatggtgac tccttgctag tctcgttgcc gatttcgaga ctaagtttat
      481 cggccatttc cgaggaatcc tcctctattt tcttaaacca ttccaaccct ccggttacct
      541 caaaaatcct ttccagctca tcgagacttt cctgtgaaat tttccttatt gccccggggc
      601 ctgagccttt atagttggcg cctacttttt ttaacacggt ttcgcggttg tccctaaccc
      661 attgccaaac agggcctagc tcaggaaaca tgcttagctc ggtatggtca aagtggacgc
      721 agtagcctgt ttcgagaatg gagttgccaa gcattcgttt aagcgagagc tgctgcctcc
      781 ctttctcgga ttctacaaat agccctgttt ctatggagag ctttcggagt gcctgaatta
      841 ttagccctgg ggcattattt cgggggcacc tggttttgca gctcatgcac tccccgcagt
      901 accatattgt atcgcttttc agcaagttct caatcacttc gtcgtcgtgg gtttgaacca
      961 gattggcaat acttcggggt tcatagtcgt aaacttctgc cgccgggcag attgcagtgc
     1021 aggttccaca gtttatgcag ccgttgattc cctcaataaa cctgacatcg ctgttcaacc
     1081 tgtcaaatag ttttcccatg ttatactatt tgcctgatag ccaaaatgat ctatcaagat
     1141 taatgttatt attgtaacag caaatgtaaa aattttacca tgatattatt taccagctaa
     1201 tatttatcaa tttttattgt agttggtaat taatgtcaat gctgctagag aagagcagct
     1261 aacttttttg agagtggctt tagccggcac cccgctaatt cgggcttggc tttcaatttt
     1321 atataaagct gtttacctgg tgcaaaattt tttgattgcc atgtaggcct cctcaacacc
     1381 tagctctccg gctttaccta agttattgca gccctcatcc ttgttgccgg taataagtag
     1441 gctaagccct ttgttaaacc atgcacagct tagaagtgga ttttctctta ctgccagcga
     1501 gaaccagcta atggcgcttt catagtcgtt cgtcataata cttagattcc ccaggttgta
     1561 agcaataaga ccatttttag ggaacacttc atataaacct tttagttcga ctatagcatc
     1621 attatatgct gtaatgcttg ttatccctgt attttttgaa gagctcatag caatgccgga
     1681 gatttctgtt gaggtgttaa aattttcaat gaattctgcc atttcaacat tattcatgat
     1741 gtaggagaag aggtaaatcg ggttactgct attttgctca aaggcctttt taaattcgtt
     1801 agtggcgcta gcaaacttgc cattttctgc taaaaatata ccctttacca gacataatgc
     1861 cggaaagttg gctatagtgg aatcggcgct ttctatggaa agcttttctg ttgagttgct
     1921 gggagcaaac ccaaagatta acttgtagtt tatgggaagt gttgcattaa tagaatcaag
     1981 gtaaacggat gcaagattgt tatttgttgc tggtttatcg ggttgattaa aatgaagcct
     2041 tatcattggc atgaatgcag aggcaatgcg aattctgttt gattcgatgc ttaatgtaga
     2101 agccaggtta aaatcgcttt caagcgaaat cagcttgttg agttttccgc tcgagtcgag
     2161 caacgaagta atttcggcct gtccctttcc atggaattta ttagctagag cttggccttt
     2221 caggaaatcc tcctcggctc ccggtaggtt acccagctgt ctttttgcat cggagcggag
     2281 gaggtaggca ttggcgaagt cgggaaaaag gcttatggcc ttggtaaaat cgtcgataga
     2341 ccgtgttgct tcaccctttt ccaggtatat agaacctctg tagtatggca ccagaacatt
     2401 ttgggaattt attgccatta gccttgtaag gtcgtcaatt gctgggtctg tatttcctgc
     2461 ctggtagtta ataattgccc tgttaaaaag cgctatttgg tagtttggat ttaatcgtag
     2521 ggcttttgta aggtcatcaa tagagttttg atatttttta aagttatagt ttataatggc
     2581 ccgggtaaag taaaagtcag ccttaaggct gtcgagctca atggctctgt ttaaatcggc
     2641 taaagcaagg tggtttctat caagttcggc gtttacagct cccctgcggg cgtatgctgc
     2701 agcatggaat gggttcacat taatggttgt gctgtagtcg cttatagcac ccagagtatc
     2761 ggctatgagc agtttggcag tggcgcggtt ataccatgcg tcggcattgg aactttcaag
     2821 ctgaattacc cgtgtgaagg ttcctattgc cttagggtag ttctgggttt ggaattggag
     2881 cacaccgagt gtgaagagaa aatcggggga gttaggtcta agctccagtg cggagctaat
     2941 ctccttcaac gccttgtcat attctgatac ctgacttaag gcaattgctt tgtagtggta
     3001 tgattgagca gatagcgggt tagcctttag cgacttgtcg aagtcggtga ttgcacccct
     3061 tatgtcgccc aggttgtact ttgccacccc tctaagaaac cagccctcga aaagggagga
     3121 gtctattccg ataagggtgt tcaaaaccgg tagcgattcg ctgtagcgcc cttgcgaaat
     3181 caactcttcg cttttgtagt agtagtaggc cttgtttagc tggccataaa gcggtgaatg
     3241 gcctaaacta ccgagtatta ttaaaaacag tataggcaga tgccgtttca atttactcac
     3301 ttttttagtt tttctttttg cttttactac ttgtatctcc tttaaggtct attcttccca
     3361 ggttgtacat gtttcgaaga atggtgcgct ggtacctgta catgtagctg ctaggcttag
     3421 ccggattacg ctttgctggg ttgggaagca cggtagccag catggcagcc tcataggtgc
     3481 taatttttga agcgggtttt ccatagtagc attccgaagc agactccact ccatatatgc
     3541 ctcttcccaa ttcggctaca ttgaggtaaa cctccataat tcgctccttg ctccaaaaaa
     3601 cttctaccag aaatgtaaag taggcctcaa atccctttct tacccatgaa cggcgcggcc
     3661 atagaaatac attctttacg gtttgctggg ttatggtgct agcgcctctt agccttttcc
     3721 cctttttatt atgctccttg gctttttgaa tggactccca gtcgaacccg ttgtgttcca
     3781 taaacttatt atcctcagca gcacacacag ccaaaactag ctggggagat attttatcaa
     3841 tgcttaccca cttgtgatta atccgattag atccgccgtc atttagcggc tgaactcgtt
     3901 tggctaccat tagcatggta aggtttggat tgataaaaga aaaagctatt accgatatga
     3961 cggttgtaat aaagaatgtg gcgatggcat ataatactaa ccgtcgaaac gattggtacc
     4021 gctgtgattt ttgtctattc ttaccattca ccgtcaaggc caagcgtgct tttttgcacg
     4081 tgctaaatta taaatttata aagtggattt caaaacagct ttaacggttg attagctcat
     4141 tcaaccattt aaaaaaaata gggttctgat tacaagtact ttagctcata acttttaagc
     4201 atcggggagg aggggacgga acacccaatg ttttggcttc acctagctag caaaaggccc
     4261 ggttgttcaa gcgtgttttg caggtttcct tacaagggcg tttcgtaatg ccatgttgat
     4321 ttttgcagaa tattccaaaa taatagaaca acctgtagct gaacaacaaa acatgttgtt
     4381 taacaatgtt gaaaaacagg caatttcgtt gccctttttc ctagttatca aaccatttca
     4441 gggtaacttt gtgccacaaa agtattaacc taaattttta ctcacatgag cgtaacccat
     4501 attgaacata tcggcattgc cgttaagagc ctaaatgatg caatccccta ctatgaaaat
     4561 ttattgggag tgaagtgcta cagcattgag gaggtaaaag accagaaggt taagacagcc
     4621 ttcttcaagg tagggcaaac caaaattgaa ctcctggaaa caacggatcc cgaaggtact
     4681 attggcaaat ttattgaaaa aaaaggtgag ggaatacacc atatagcctt tgctgtaaag
     4741 ggtataaacg aggagctgca acgtatgcag gagaatggca tagttttaat tgataaaact
     4801 ccacgcagtg gtgcggaagg gttggagata gccttcctgc atccaaagtc cacctttgga
     4861 gtgctgaccg aattctgtga agatagaaat cagaaatagt ttatccttaa aaataacgat
     4921 atgagcattc aggagcaaaa aataaaagag cttattgagc tgcgcgaaaa agctaagcta
     4981 ggtggaggtg cccgacgtat tgaatcccag cataagaaag gaaaatatac tgcccgcgaa
     5041 cggatagaga tgcttctcga cgagggaagc tttgaggagt tcgacatgtt tgttacccac
     5101 cgctgtactg attttggcct ggataaggaa acctacctat cggatggcgt aatcacagga
     5161 tatggaacta ttgacggacg cttggtttat gttttttcac aggactttac ggtgtttggt
     5221 ggctcgcttt cagaaatgtt tgccgccaag atttgcaagg tgatggacat ggccatgaag
     5281 gttggtgcgc cggtcattgg aataaacgat agcggtggag cccgtattca ggagggggta
     5341 accagcctgg gagggtatgc cgaaatattc gagcgaaaca tcctggcttc tggggttgtt
     5401 cctcaaattt ctgcaatatt tggcccctgc gctgggggtg ctgtctattc tccggcatta
     5461 accgatttca tcgttatgtc gcgaaacacc agctatatgt ttgtaactgg ccccaaggta
     5521 gttaaaacgg taaccggcga aaccgtaact tcagaggaac ttggtggtgc cggcgttcac
     5581 tcgtccaaat cgggagtggc tcactttgtt gccgaaaacg aggaagaggg cataatgctt
     5641 atcaggaagc tgctgagcta tttgcctcaa aataacctgg aggaagcacc aataatgccc
     5701 tgcgacgatc caatcgaccg ccttgaggat tccctcaacg atattattcc cgataaccca
     5761 ggtaagccc
//
LOCUS       DAOPHX010000141         5768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_18315_length_5768_cov_5.430247, whole genome shotgun sequence.
ACCESSION   DAOPHX010000141 DAOPHX010000000
VERSION     DAOPHX010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18315_length_5768_cov_5.430247"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PK502_09315"
     CDS             complement(<1..841)
                     /locus_tag="PK502_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPH01055.1"
                     /translation="MAHIDAGKTTTTERILFFTGKTHKIGEVHDGAATMDWMVQEQER
                     GITITSAATTTFWRYNDQQYQVNIIDTPGHVDFTVEVERSLRVLDGTIATFCAVGGVE
                     PQSETVWRQADKYRVPKIAFVNKMDRVGADFLSVVGQIREKLGANPVPIQLPIGAEDT
                     FVGIVDLIENKAYVWNDDQKSNSNNFRVEEVPDSMKQEVAEWRSKLVEAVASYNDDLL
                     ERFFDNPESISNDEIIDTIRRATIDMSIVPVLCGAAFKNKGIQKLLDAVIAYGDLGKS
                     GNKV"
     gene            complement(914..1390)
                     /gene="rpsG"
                     /locus_tag="PK502_09320"
     CDS             complement(914..1390)
                     /gene="rpsG"
                     /locus_tag="PK502_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPH01056.1"
                     /translation="MRKSKPKKRLILPDPKFGDVMVTRFVNNLMERGKKSVAFDIFYQ
                     SLDIVGQRMKDADKNPLEIWKKALENITPQVEVKSRRVGGATFQVPMEVRPERKISLS
                     MKNLILYARKRSGKGMSEKLAAEIMAAYNEEGAAFKRKEDMHKMAEANKAFAHFRF"
     gene            complement(1423..1800)
                     /gene="rpsL"
                     /locus_tag="PK502_09325"
     CDS             complement(1423..1800)
                     /gene="rpsL"
                     /locus_tag="PK502_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPH01057.1"
                     /translation="MPTIQQLVRKGRSKMVDKSKAPALDACPQRRGVCVRVYTTTPKK
                     PNSAMRKVARVRLTNGKEVNAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHLIRGTL
                     DASGVEGRKQRRSKYGAKRPKKK"
     gene            2306..4462
                     /locus_tag="PK502_09330"
     CDS             2306..4462
                     /locus_tag="PK502_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPH01058.1"
                     /translation="MKTYSTDQIRNIALIGNTGSGKTSLAESMMFEGKVIERKGSIEG
                     KNTISDYTEIEQLNQRSIFSTVLYTEYMDHKLNVIDTPGSDDFVGSVITSMYPTDMVV
                     VTINAQYGVEVGTEIFNRHAERLERPILIAVNQLDHEKANFEQAMDSLRQTYGTKVLQ
                     TQYPLNTGSLFDSFIDVITMKMYKFKDDTGIREEFPIPAEEMEKATALHNTLIESAAE
                     NDETLMEKFFEQGSLSEEEMRKGLSIGVRNRQIFPVFCLSAKKNIGTKRLMEFIIKIG
                     PSPASTPMLKNNSKTEIKCDAAAPTSLFIFKSTVEPHLGEINYFRVMSGKIAEGMDLT
                     NANNSTKERLTQIFAVAGKNRTKVSEMVAGDIGATVKLKNSKTNQTLCGPGIDFTFEP
                     MQFPNPKFRTAIKPKNEAEVEKLGELLNRAHQEDPTIEVEHSKELKQIIISGQGEHHL
                     NILKWQLTNLNKLEVEFIPPRIPYRETITKIAQADYRHKKQSGGAGQFGEVHLVIEPH
                     VEGAPDPVKYKINGKEINLSVRGKEEIKLDWGGKLIFYNCIVGGSIDARFMPAILKGI
                     MEKMEQGPLTGSYARDIRVAVYDGKMHPVDSNEISFKLAGRNAFKNAFKEAGPKIMEP
                     IYDVEVLVPSDRMGDVMSDLQNRRAIIMGMGSTKGFEVINAKVPLAELNKYSTILSSL
                     TSGRATYTMKFASYEQVPTDVQEKLLKAYEAEEEEE"
     gene            complement(4700..5539)
                     /gene="lgt"
                     /locus_tag="PK502_09335"
     CDS             complement(4700..5539)
                     /gene="lgt"
                     /locus_tag="PK502_09335"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPH01059.1"
                     /translation="MLVFIHWNVNPEIFSIGNLSVRYYGLMWALAFYLGYVIFNRFVK
                     KEGLPNGFLDSLTMYMLIGTVLGARLGHCLFYEPSYYLSHPLEILKIWKGGLASHGAA
                     LGILIALYLFGRKHKVPMIYVVDRVVITVALGGALIRIGNLMNSEIYGVKTNLPWGFI
                     FERNNEIYPKHPTQLYEAISYLAIFFLLHYIYRKNEGKLKTGFIFGLFLVLLFMARFL
                     IEFLKEPQVDFEAKMTLNMGQLLSLPFIAVGIAFIIYSQVKPSYYTPPKQTPVQKSKK
                     SKG"
BASE COUNT         1669 a         1323 c         1250 g         1526 t
ORIGIN      
        1 taactttatt accagactta cctaaatctc cataagcaat aacagcatca agcaactttt
       61 gaattccttt gttcttaaaa gcggcaccac agagaaccgg aacaatggac atgtcgatag
      121 ttgcccgccg aatggtatcg ataatttcgt cgttagaaat tgactcgggg ttgtcgaaaa
      181 agcgctccag caagtcatcg ttgtaggagg caactgcttc aaccagcttg ctccgccatt
      241 cggcaacctc ctgcttcatg gagtcaggaa cttcctctac cctaaaattg ttggagttgc
      301 ttttttggtc gtcgttccac acataggcct tattttcgat aaggtcaaca ataccaacaa
      361 aggtatcctc ggcgccaata ggcagctgaa taggaactgg gttggccccg agtttctccc
      421 taatctgacc aacaacagat aggaaatctg ccccaacacg gtccatctta ttaacaaaag
      481 cgattttggg aacccggtac ttgtcggctt gtcgccacac cgtctcgctt tggggttcaa
      541 caccgccaac tgcacagaaa gtggcaattg ttccgtcgag aacccgaagc gagcgttcca
      601 cctcgacagt aaaatcaacg tggccaggag tatcgatgat gtttacttgg tactgctggt
      661 cgttgtatct ccaaaatgta gtagtggcag ccgaggtaat ggtaattcca cgctcctgct
      721 cctgcaccat ccaatccatt gtggcggctc catcgtgcac ctctccaatt ttatgggttt
      781 tcccggtaaa aaagagaatg cgctcggtag tagttgtttt accggcatca atgtgcgcca
      841 ttattcctat gttgcgagta tattttaaat ccctgctcat ctatattcct accttcctcg
      901 tgctgtggta ttgttaaaaa cggaaatggg caaaagcttt gttggcctca gccattttat
      961 gcatatcctc tttcctctta aatgcagctc cttcctcgtt gtaggccgcc ataatttcgg
     1021 cagcaagctt ttcgctcatt cccttaccgc tacgtttacg ggcataaaga atgaggtttt
     1081 tcatggaaag cgatattttt cgctcgggac gaacctccat aggaacctgg aaagttgccc
     1141 cccctaccct gcgacttttc acttcaacct gtggggtgat gttctccaag gctttcttcc
     1201 atatttcaag cgggttctta tcagcgtctt tcatccgttg tcctactatg tcgagcgatt
     1261 ggtagaagat gtcgaatgca acgcttttct tgccgcgctc catcaggttg ttaacaaacc
     1321 tggtaaccat cacgtcgcca aatttaggat cgggcagtat taatcttttt tttggtttag
     1381 attttctcat cgctgtatat aatcaaatga ggttaacatt acttactttt tcttaggccg
     1441 cttggcgccg tactttgagc gtcgctgttt gcgcccttca actcctgaag catcgagtgt
     1501 tccgcgtatc agatggtaac gaactccagg taaatctttt accctgccac cacgaatgag
     1561 cacaatggag tgctcttgca ggttgtggcc ttctccagga atgtaggcgt taacctcctt
     1621 gccgtttgta agcctaaccc tggcaacttt tcgcatagcc gaatttggct ttttaggagt
     1681 agtggtgtac acccgtacac ataccccgcg tctctgggga caagcatcca gcgcaggcgc
     1741 cttgctctta tccaccattt tagaccttcc ttttcttact agctgctgaa ttgtgggcat
     1801 aaatacagtt tatgttaaat tggttttaca aaataggtgc gcaaaggtat tgattttttc
     1861 aataccatcg ttcaacttgt tatttttttg ctgttattca gcaagtttac ctgccgacaa
     1921 ctaattttgc gggtgcaaag atatactttt ttaacataaa aagccatcgg cactcttcaa
     1981 ccttgaaagc agcagctaat aaaattggcc aggattagta gcattaccct aataatcagc
     2041 tgcttaattt gaataaaaaa aagaatgctc agggttgatt tttatactgt atccctggat
     2101 aaagaggcaa ggcaaccatt tttttataga atcacagaac aaacttgcat atttcaaaat
     2161 tttactattt ttgggggcat tcaacaatcg ccaaaggcta ctagagccgt atttcttaca
     2221 ttacctttat aaaataaata atagtaggtg ctgggattga gacgatagca gaaaagaatg
     2281 tcaaacacta aatagcatat tacgcatgaa aacttactca acagatcaaa ttagaaatat
     2341 agcccttatt ggaaataccg gttcggggaa gacgtctcta gccgagtcca tgatgtttga
     2401 agggaaagtt attgagcgga aaggttctat tgaagggaag aataccattt cggactacac
     2461 cgaaattgag cagctcaacc aaaggtcaat tttttcaaca gtgctgtaca ccgagtacat
     2521 ggaccataag ctcaacgtaa ttgatacacc ggggtcggac gattttgttg gaagcgttat
     2581 cacctcaatg tatccaaccg atatggtggt ggtaaccatc aacgcccagt acggagtgga
     2641 agttggtacc gagatattca accgtcatgc cgaaaggttg gaaaggccta tccttatagc
     2701 agttaaccag ctcgaccacg aaaaggccaa ctttgagcaa gcaatggatt cgcttaggca
     2761 aacctatggg actaaagtac tgcaaaccca gtatccgcta aacaccggga gcctatttga
     2821 cagcttcatt gacgtaatca ccatgaagat gtataagttt aaggatgata caggcattcg
     2881 cgaagagttc ccgataccag ccgaggaaat ggaaaaagct accgcccttc acaataccct
     2941 catagaatcg gctgccgaaa atgacgagac tttaatggaa aaatttttcg agcaaggttc
     3001 cctttccgaa gaggaaatgc gcaagggtct atctatagga gttcgcaacc gtcaaatttt
     3061 ccccgttttc tgcctgtcgg ccaaaaagaa catcgggaca aaacgactca tggagtttat
     3121 cattaagatt ggcccatccc cggcttctac acccatgctt aagaacaaca gcaaaacgga
     3181 aattaaatgc gatgccgctg cgccaacatc tctattcatc ttcaaatcta ctgttgaacc
     3241 gcatctaggt gaaatcaact acttccgggt tatgtctggt aagattgctg agggaatgga
     3301 cctgaccaat gctaacaact ccaccaagga acggttgacc cagatttttg ccgtagcagg
     3361 aaaaaatcgg acaaaggtat cggaaatggt ggctggcgat attggggcta ccgttaagct
     3421 aaagaattcc aaaactaacc aaacgctctg cgggcctggg attgatttca catttgaacc
     3481 catgcaattc cccaatccaa aattccgaac cgcaataaaa ccaaagaatg aagccgaggt
     3541 tgagaaactt ggagagctgc ttaaccgtgc gcaccaggaa gatccgacca ttgaagtgga
     3601 acattcaaag gagcttaagc agataataat ttcgggacag ggtgagcatc acctcaacat
     3661 ccttaaatgg cagcttacta acctcaacaa gctagaagtt gagtttatcc ctccgcgaat
     3721 accctaccgt gaaaccatca ccaaaattgc tcaagccgac taccgccata aaaaacaatc
     3781 gggaggtgca ggtcagtttg gtgaggttca cctggtaatt gaaccccatg tggagggagc
     3841 ccccgatcct gttaagtaca aaataaatgg caaggaaatt aacctttccg ttaggggtaa
     3901 agaggaaata aagctggact ggggcggaaa gctgatattt tataactgta ttgttggtgg
     3961 atctattgat gcccgcttta tgcctgccat tctaaagggg attatggaaa agatggaaca
     4021 agggccgctc accggttcgt atgcgcgcga catccgggtg gccgtttacg atggcaaaat
     4081 gcacccggtt gactctaacg aaatttcatt taagctggct ggtagaaatg cctttaagaa
     4141 tgcttttaag gaggccgggc caaaaattat ggagcccatc tacgatgtgg aggtgctagt
     4201 tccttccgac cgcatggggg atgtaatgag cgatctgcag aaccgcaggg caattatcat
     4261 gggaatggga agcacaaagg gttttgaggt gattaacgcc aaagttcccc tggcagaatt
     4321 gaacaagtac tccaccattc tgtcgtccct aaccagcgga agggcaacct acaccatgaa
     4381 gtttgcctca tacgagcagg ttccgactga tgttcaggaa aaattgctga aagcatacga
     4441 ggccgaagaa gaagaagaat aaatctggct tttacaagcc aaccaactta tcagggctac
     4501 cgggttaaca tccggttgcc ctttcttttt gaaactaact aaagagcttt aggatagtat
     4561 gccacaacta cccttacaaa aagtagcttg taaccttctg aaagaattga gaagaaagtt
     4621 gaactcctgt tccaaccgta taaatttacc ttttaaagta cttgcagcca ggtaaatgaa
     4681 ccgaataaaa ctcccaattc taccctttag actttttcga cttttggaca ggtgtttgct
     4741 taggcggtgt gtagtaggaa ggtttcacct gcgagtagat gatgaatgca atgccaactg
     4801 caatgaacgg taagctcagc agctgaccca tgttcaaagt catttttgcc tcaaagtcaa
     4861 cctggggttc cttgaggaac tcaattagga agcgggccat aaacaagagc accaaaaata
     4921 acccgaaaat aaaacctgtt ttaagcttac cttcattttt acggtaaatg tagtgcagaa
     4981 ggaagaaaat ggccagatag ctaatagcct cgtaaagttg agttgggtgc ttggggtata
     5041 tctcgttgtt ccgttcaaaa atgaaacccc acgggaggtt tgttttgact ccatatatct
     5101 cggagttcat caggttgcca atccgtataa gggcgccacc cagcgcaaca gtaataacca
     5161 ccctgtcaac aacataaatc atggggactt tatgctttcg cccaaaaagg taaagagcaa
     5221 taagaatccc cagggcagcc ccatggctgg ctagtcctcc tttccatatt ttaagtattt
     5281 ccaaaggatg gctaaggtag tatgatggtt cgtaaaaaag gcagtggcca agtcgggcac
     5341 caagaacggt tcctatcaac atgtacatgg taagcgagtc gagaaatccg ttgggcaatc
     5401 cctccttttt cacaaagcgg ttgaaaataa cataacccag gtagaatgcc aacgcccaca
     5461 ttaggccata gtaccgaacc gagagattcc cgatactgaa gatttcagga tttacattcc
     5521 agtgtatgaa taccagcata ggttaaacta ttatttaatc cggtcaaaag tagtgttttt
     5581 ttgcttttga ttttgatttt aagctaaaac tgccatgctt taaaaatggt taaactaatg
     5641 taattaagcc aaaaaaacca acaggacaat ggcagcaaaa ccaattcgga taattccaac
     5701 cgagtaggta ggaggattac tcccccacac tcccccaccc tctcacacca cccggcttcc
     5761 gcctgcgg
//
LOCUS       DAOPHX010000142         5744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_18429_length_5744_cov_5.489190, whole genome shotgun sequence.
ACCESSION   DAOPHX010000142 DAOPHX010000000
VERSION     DAOPHX010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18429_length_5744_cov_5.489190"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PK502_09340"
     CDS             complement(<1..818)
                     /locus_tag="PK502_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HPH01060.1"
                     /translation="MVKFILQQSLNPYFNIASEEYLLRSFSDDFIYIYRCNPSIVVGK
                     HQNTFAEVNIRLVERLSIPVVRRLSGGGTVFHDLGNINFCFISNGVPGQLVDFKRFTL
                     PVIDFLRSLGVNAYLGQKHDIRVGDRKISGNASYAFGNRVMHHGTLLFSSDLEFLAEA
                     IRVEPGLFTGSAIQSNRSRVANTVDFLSPKMDIEQFTNRMMEWFAWKVPNGLPYQLSD
                     ADTLAVDLLVKEKYSTWGWNYGFSPSFERICPLTTSLGAVTMRISVAHGHVESTA"
     gene            891..1298
                     /locus_tag="PK502_09345"
     CDS             891..1298
                     /locus_tag="PK502_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HPH01061.1"
                     /translation="MTTYESKIVAISKRAEDIYGMLSDFRNFTPMVAQRVEEWQAEEQ
                     RCSFKYQGKGPVGLKIVEKEKNKTIKITGDDNLPMQFYLWIQLKEVAPYDTRMKITLK
                     AELNMMMKMLLGKKLDEGVNAMAEGIAAAFNTI"
     gene            complement(1320..4607)
                     /locus_tag="PK502_09350"
     CDS             complement(1320..4607)
                     /locus_tag="PK502_09350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPH01062.1"
                     /translation="MSSRFTLHFNVTMSIYTRYILAVVLALIGYPKMPQAQERGLFLS
                     RYFPASEYNGSTQNWCAVEDNRGVMYFANGQGVLEYDGATWNLIRVDNESAIRSLAAD
                     SSGRIYVGAFGEMGYLYPNRSGQLRYMSLMSLLDKNHSRFSQIWNIYTFGDTAFFLSD
                     SCLYRYCNGKFSYWTSQEGCFYFAFKFNNHLYIQETGVGLQQFDAESLVLVEEGNLLA
                     SQRIHAILPFGNEMLVCTRSNGLFLFRNEGVKNRLTPVSHLSGRALAVDRYFKEYSFY
                     HGVAINDNLLALSSINGNLLMVDTTWLVREVIDYRTKGVKSPVNYLYLQKNNLLWLAL
                     DNGICVVEINSPFRYWTDDMGVNGVVTDVAKLGGFLYVSTQVGNFFLDEKKEEDFMPD
                     MFKKVEGDFEQSWEYLYFKVSSNLFPQGHSRPADTLLLVATRTGVYRINGSRSSRISR
                     YEPVFCFHQSKANPAALLVGLSSGIAIVEYRDGFWVDKGFVPGIKENIEQIAEDLDGN
                     VWLKASFSGVYRLKNLFFGSSFKGSLVVEKYDTSSGLPDLKSLYMTSHADSLFFYSQN
                     GYYTFNERLRQFQAYNPANNRRFSAENVDTLAWRRVWNNIISGFYVAHTSDSIIWFNT
                     PKGLFRYTPSSYNRCFDAPATFIRKVVNLDSTLFYGTNFESSKVDSAAGKPAFLPSHN
                     PLVDLGTVLEYKRNSLIFNYASPYFEGDDPVQFCYQLVGYDKLWSAWTTSNRKEYTNL
                     PEGFYTFKVKAKNYMGIEGAPAEFRFRVKPPWYRTALAYFGYMIIAFLLIAGIIRAYT
                     YHLVLEKNKLEQLVAERTQEILQQKEEIQVQAEHLKSANERILLKNAELEAQKNEIEN
                     QKNKLEESYTTMNTFFGVIAHDLRNPISILVNFMGILLSDFDSMDRKKVVAYIEELNK
                     LALNTYNLLENLLDWSKSQMGSISCNLKPVNLKSLVIQNVELVQGKIEQKKVNLRVMI
                     PEECIVSADENMLHTIVRNLISNALKYTYDNGEILIYAELRNSTCYLSVKDNGVGISS
                     ELKGKLFKADRDATTPGLHNEKGSGLGLILCKTFVEQMGGNILVESQPGLGSTFTVTL
                     KLEGIPG"
     gene            complement(4799..5509)
                     /locus_tag="PK502_09355"
     CDS             complement(4799..5509)
                     /locus_tag="PK502_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPH01063.1"
                     /translation="MSYKCVLVDDEPLAVGVLEGFLRHIPDIEVVATFNEAIPLFDFI
                     QHHKVDIIFLDIEMPNLSGIDFLKCLKDPPFVIITSANRDYAVDGYELNVVDYILKPL
                     TMERLVRAVSRAAEALELKARNVTAAPDAEYIFLKEGKKFVRVKVDEIQYIESIKDYV
                     KVVAEGKTVVTRMNISAVEKLLDPAEFIRVHRSFIISKKHIDAYTATSIEIGSVEIPV
                     GRIYRDEAVRRLGYDFEV"
BASE COUNT         1678 a         1360 c         1171 g         1535 t
ORIGIN      
        1 gcggtgctct caacatgccc atgggctaca ctaatacgca ttgtaacagc ccccaaagat
       61 gttgtcagag gacaaatacg ctcgaatgat ggcgaaaatc catagttcca gccccaggta
      121 ctatacttct ctttcaccag tagatccacc gccagggtat cggcatctga cagctggtag
      181 ggtaaaccat tgggtacttt ccaagcaaac cattccatca tcctgttagt aaactgctcg
      241 atatccattt tcggcgaaag aaaatcaacc gtattggcaa ctctgcttct gttcgattga
      301 atggcactac cggtaaaaag ccccggttca accctaatag cttcggcaag aaactccaaa
      361 tcggatgaaa acaggagagt tccgtgatgc attacccgat taccaaaagc atagcttgca
      421 ttgcccgata ttttcctatc gcccacccga atgtcgtgct tctggcccaa ataggcatta
      481 accccgaggc tacgaagaaa atcgataacc ggtaaggtaa aacgtttaaa atctaccagc
      541 tgcccaggga ccccattcga aataaagcaa aagttaatgt tgcctaaatc gtgaaacacg
      601 gttcctccac ccgatagcct tctaaccaca ggaatggaga gccgctccac caaccgaatg
      661 ttgacctcgg caaacgtatt ttggtgctta ccaacaacta tcgaggggtt gcatcggtag
      721 atgtagataa aatcgtcgct aaagctgcgc agcaggtatt cctccgaggc aatgttaaag
      781 tagggattta ggctttgctg aagaataaac ttaaccatgg agtaagcacc gggggctaac
      841 cggaatactt aaattttcac ttaatttgca tcgacaaatt taggcaaaaa atgaccacat
      901 acgaaagtaa aattgttgcc atctccaagc gcgctgagga catttacgga atgctttcgg
      961 actttagaaa ttttactcca atggttgccc aaagggttga ggaatggcag gccgaggagc
     1021 aaagatgtag ctttaaatac cagggaaaag ggcctgtagg gctaaagatt gtggaaaagg
     1081 aaaaaaataa aaccatcaag attaccggtg atgataacct ccccatgcaa ttttatcttt
     1141 ggattcagct aaaggaggtt gctccctacg atacccgaat gaaaattacc ctaaaggctg
     1201 aactcaacat gatgatgaaa atgttgctgg gtaagaagct cgatgagggc gttaatgcca
     1261 tggccgaggg aattgcggca gcattcaata ccatttaaat gggtagtatt gaggagcggc
     1321 tagccaggta ttccctctag ttttaatgta acggtaaacg tgcttcctag tcccggttgg
     1381 ctctccacca ggatgtttcc gcccatttgt tctacaaacg ttttgcagag tatcagccca
     1441 agccccgaac ccttttcgtt gtggagaccc ggagttgttg catctctatc ggctttgaat
     1501 agcttccctt taagctcgct gcttatacca accccattat ccttcacgct caggtagcat
     1561 gtggagtttc taagttcggc gtaaatcaat atttccccgt tgtcgtaggt gtacttcaag
     1621 gcattcgata tcaagttccg aacaatagtg tgcagcatat tttcatcggc tgaaacgatg
     1681 cactcttcag gaatcatcac tctcaggtta accttttttt gttcaatttt accctgcacc
     1741 agctcaacat tctgaatcac cagcgattta aggttaactg gcttaagatt acagctgatg
     1801 ctgcccattt ggcttttcga ccaatcgagc aggttctcca acaggttgta cgtgttgaga
     1861 gccagcttgt tcaactcctc gatataagct acaacttttt ttctatccat gctgtcaaaa
     1921 tcggaaagta aaatccccat gaagtttacc aggatactta tagggttccg taaatcgtga
     1981 gcaatcacac caaaaaaggt attcatggta gtgtagcttt cctccagctt gtttttctga
     2041 ttttcaattt cattcttctg ggcttcaagc tcagcattct ttagcaaaat tctttcgtta
     2101 gcactcttga ggtgttcggc ctgcacctga atttcctcct tctgctgcaa aatttcctgg
     2161 gttctttcag caaccagctg ctccagctta ttcttttcaa gaaccagatg gtatgtatat
     2221 gcccgaatga ttccggcaat gagtagaaaa gctataatca tatagccaaa gtatgccaag
     2281 gcagttcggt accaaggagg tttaactcta aaccgaaatt cggcaggagc gccttcaatt
     2341 cccatgtaat ttttagcctt aactttaaaa gtgtagaagc cctctggtag gttggtatat
     2401 tccttgcggt tactggttgt ccaagccgac cagagcttat cgtaaccaac tagctgatag
     2461 cagaactgca cggggtcgtc tccctcgaaa tatggtgatg cgtagttgaa tatcagagag
     2521 ttacgtttat actccaacac tgtccccaga tcaacaaggg gattatggct aggtaaaaaa
     2581 gctggttttc cggcagcact gtccactttc gaactttcaa agttggttcc atagaatagc
     2641 gtggaatcca ggtttactac ctttcgtata aaagtagcag gagcatcaaa acaacggttg
     2701 tagctgcttg gagtgtaccg aaataacccc tttggggtgt taaaccatat gatggaatcg
     2761 gaggtgtgag caacatagaa gccagaaatg atattattcc atacccttct ccaagccaga
     2821 gtatcaacat tctcggcgga aaatcgccgg ttgtttgctg gattgtacgc ttgaaattgc
     2881 ctcaacctct cgttaaaggt gtagtacccg ttttgggagt aaaaaaagag tgaatctgca
     2941 tggctagtca tgtataagga tttcaggtcg ggcaaaccac tcgaggtatc atacttttca
     3001 accaccaagc tacccttaaa gctactgcca aagaaaaggt ttttaagcct gtaaactccg
     3061 ctaaagctag ccttcaacca tacgtttcca tctaaatcct cggctatctg ctcaatattc
     3121 tccttaatcc caggcacaaa ccctttatct acccaaaatc catcacggta ctcaacaata
     3181 gctatcccac tagaaaggcc caccaggaga gcagcaggat ttgctttact ttgatgaaag
     3241 cagaaaaccg gttcatacct tgatattctg ctcgaccggc tgccattaat tcgatataca
     3301 cccgttcggg ttgccacaag caaaagggtg tcagctgggc ggctatgacc ctgagggaat
     3361 aaattgctag aaaccttaaa gtaaaggtat tcccacgact gctcaaaatc accttcaacc
     3421 tttttgaaca tgtcgggcat aaaatcttct tccttttttt catcaagaaa aaaatttcca
     3481 acttgggttg agacatacag aaaaccgcca agcttagcta catcggtaac cacaccgtta
     3541 acccccatgt catcagtcca gtagcgaaat ggcgaattaa tctcaaccac acaaattcca
     3601 ttgtcgagag ccaaccagag caggttgttt ttttggaggt acaggtagtt aacggggctc
     3661 tttactccct ttgtcctgta atcaataacc tccctaacca gccatgttgt gtcaaccata
     3721 agtaaattgc cattaatgga gctcaaggcg aggagattat cgtttattgc cactccatgg
     3781 tagaatgagt actccttaaa ataacggtca actgctaaag ctctgcccga taggtgcgag
     3841 actggggtga gccgattttt taccccttca tttctaaaga gaaacaagcc attactccgt
     3901 gtgcaaacca gcatttcgtt cccaaagggt aaaatagcgt gtatccgttg cgatgcaagc
     3961 agattgccct cttcaacaag cacaagagac tcagcatcaa attgctgaag cccaactcca
     4021 gtttcctgaa tgtaaaggtg gttgttgaat ttaaaagcaa aatagaagca accttcctgg
     4081 ctagtccagt agctgaactt gccattacag tacctgtaca gacagctgtc ggaaaggaaa
     4141 aatgctgtat ccccaaaagt gtagatattc caaatctggc taaacctcga atgattttta
     4201 tccagcaagg acattagcga catataccgc agctggccac tccggttggg gtaaagatat
     4261 cccatttctc caaaggctcc cacgtatatc ctgccggagg aatcggctgc caggctccgt
     4321 attgcagatt cgttatcaac cctaatcaga ttccaagttg caccgtcata ctcgagaacg
     4381 ccctgaccat ttgcaaaata catcaccccc ctgttgtcct caacagcaca ccaattctgc
     4441 gttgagccat tgtactcgct tgctggaaag tacctcgata aaaacaggcc tctctcctgc
     4501 gcttgcggca ttttaggata accaatgagt gccaaaacga ctgccagaat ataccgggtg
     4561 taaatgctca tggtaacgtt aaagtgtaaa gtaaaacggc tactcataac attttttttg
     4621 tgttgatagc agggattcgc tccccgacaa catcattatg ggttcccctc tccccaaatc
     4681 tagttattta aaccccaagg cgcacccctc ccttgcggaa agaactctgg tgtagggcta
     4741 aagccaacaa atggcaatgc caaacgcggt acatcaaaag ccatcgcctg gcagcttgct
     4801 aaacttcaaa atcatagcca agccgcctga ctgcttcatc ccgataaatc cttccaacag
     4861 gaatttcaac cgaaccaatt tcgatagagg ttgctgtgta ggcgtcaata tgtttctttg
     4921 aaataataaa tgaccgatgc acccttataa actcggcagg atcaagcagc ttttcaaccg
     4981 cactaatatt cattcgtgta actactgttt ttccttcagc taccactttc acataatcct
     5041 taatactttc aatgtattga atttcatcaa ctttaacccg aacgaatttt ttaccctcct
     5101 ttaaaaagat atactctgca tctggagcag ccgttacatt cctagccttc aattccaggg
     5161 cctcagcagc tctactaacc gcccttacca atcgctccat ggtaaggggt ttaagaatgt
     5221 agtcaacaac gttaagctcg taaccatcaa cggcatagtc gcggttcgcg gaggtaatta
     5281 tgacaaaagg aggatccttt aggcatttta ggaagtctat tcccgaaagg ttgggcattt
     5341 caatatcgag aaaaatgatg tcaaccttgt gatgctgaat gaagtcaaaa agcggtattg
     5401 cctcgttaaa agtggctaca acttcaatgt caggaatgtg ccttaagaag ccttccagga
     5461 caccaacggc cagcggctca tcgtcaacca aaacacactt atagctcatc ctgcactttc
     5521 tatagtaagt tctgaaaaat aattgggtgg caatttaaaa aaatgttaag aaaactcaac
     5581 ataaaaatga aatacataaa ggtagtttag gtagaggtat cccttgggac gatagctact
     5641 aatcttaagc ctgtttaaga atgaaaaaat aaatgttgaa tgaagtattg ggcggaattg
     5701 gctaatttaa aattacggta actgtccggc taccgacgga tttc
//
LOCUS       DAOPHX010000143         5597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_19112_length_5597_cov_6.800253, whole genome shotgun sequence.
ACCESSION   DAOPHX010000143 DAOPHX010000000
VERSION     DAOPHX010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19112_length_5597_cov_6.800253"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..758
                     /locus_tag="PK502_09360"
     CDS             81..758
                     /locus_tag="PK502_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01064.1"
                     /translation="MKHQTIFLILSCIILSSCTATKLLTSNVQPTEVTNLKLLEPYSY
                     ISMIKKGNRGQLDDSISSISKQLNINVLKSFNGQIPITGDIILIDSTINKDLEKECEN
                     LILTAERNKSISNLKITPTLDKILEANDTRFGLIIICTGFTRLKGNYGKEIAKGAALG
                     ILTLGMYYQIPVKAYSTIYAMIVDSKEDNVAFYRKSFKQDLEPLDQEVLTNQYKKIFE
                     GYFWTTK"
     gene            954..1319
                     /locus_tag="PK502_09365"
     CDS             954..1319
                     /locus_tag="PK502_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01065.1"
                     /translation="MKRNVTRKIIGGLSLTSAMFVFQACYGTPQDFGLDILIEGQVKS
                     KTSGLPIKGIKVSVADNVQYEVTDEEGKFSFYTEILEGLTLHFQDIDSTQNGLYIDKD
                     TVLTDLKENIYLDIALEEK"
     gene            1320..2399
                     /locus_tag="PK502_09370"
     CDS             1320..2399
                     /locus_tag="PK502_09370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04133 family radical SAM/SPASM protein"
                     /protein_id="HPH01066.1"
                     /translation="MLSSIKKYSFRKFRDSETKRHELNYLFWECTTRCNLNCLHCGSD
                     CSKDSLHRDMPAEDFFQAIDTIENIPQNFTVVFTGGEPLLRKDIELCGKELRKRGFKW
                     SIVSNGHLYDKQRHISLLNAGIGALTISLDGLKESHNWLRNNEKSFDKVINAIELASS
                     SNRINFDIVTCVNQKNINELEQIRELLISKNVKAWRLFTIIPIGRAAHNPDLSLADNQ
                     FVQLMDFISETRKSKGIDVKFSCEGYVGKYELLVRDSYFFCRAGINIGSILIDGSISA
                     CPNIDRSFSQGNIYKDNFYKVWENQFQPFRNREWTRIGQCELCKDFKDCQGNGFHNWH
                     GDKKNVLVCHNEKIEKQRNANILYK"
     gene            2901..3932
                     /locus_tag="PK502_09375"
     CDS             2901..3932
                     /locus_tag="PK502_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPH01067.1"
                     /translation="MFRISSLTSALFFSLAILAACVPAKEFQALQDKNAKCEEEREKL
                     STENEDLSSRSAELQAKNILLEAKVSTLASDSAICAVELSKTREQLATLNERYADLQS
                     IQDGVISGSKDETRKLLAQIQKNQEELQQKEDALKELERKLYQRKLGLDQVQANLDKM
                     SKDLEERNARLVELERMLNSKDSLMNALRKKVADALFGFEGKGLTVSIQNGKVYVSLD
                     EKLMFKSGRYEIDAKGAAAIKQLVPVLEQNPDINIMVEGHTDDVPYRGTGDLMDNWDL
                     SVKRATTIVRLLLNGSKIDPVRVTAAGRSHFLPVDRAKTPEARQKNRRTEIILTPKMD
                     EILKLLEAN"
     gene            4042..5139
                     /gene="ychF"
                     /locus_tag="PK502_09380"
     CDS             4042..5139
                     /gene="ychF"
                     /locus_tag="PK502_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPH01068.1"
                     /translation="MGLQCGIIGITNVGKTTIFNCMSSTKAAASSFAFSASKSNISII
                     NVPDSRLYELEKFQPTERIVHTTVEIVDIPGLAKGSNKGEGVGNKFLADIRNCDALIH
                     VLRCFDDPNLPHVDGSVDPVRDIETVNFELQVRDLESIEKKLQRLEKAAKVGDKEAKQ
                     AAAVLGVYKNHLESFQNAATAPVDEQDKKYVEDLFLLSTKPVIYVCNVDDQSAVTGNH
                     YVDKVREFLKNEQTEILVIAGKLEAEIAELENEDDRLAFLSDVGLTEPGVNKLIRAAY
                     SLLNMETFFTVGPKEIRAWTIKKGMTAPQAAGVIHSDLERGFIRAEVMKYNDFITLGS
                     EQACKEKGKLFVEGKNYVVEDGDILHIRFNV"
BASE COUNT         1774 a         1072 c         1243 g         1508 t
ORIGIN      
        1 gcagcaagta gaagcatact acacaataag atagatagtt acatcatact ccacttaata
       61 aatcaaacaa aatataaaaa atgaaacacc agaccatctt tctgattttg agttgcatta
      121 ttttatcatc ctgcacagca accaaacttt taacatcaaa tgtccaacct actgaagtta
      181 ctaatcttaa actccttgaa ccttattcct atatttcgat gataaaaaag ggtaataggg
      241 gacaattaga tgacagtata tcatcaattt caaaacaact aaatatcaat gttttgaaaa
      301 gtttcaatgg acagattcca attacagggg atattatcct gattgactct acaattaaca
      361 aagatctgga gaaagaatgc gaaaatttaa ttctgactgc tgaaagaaac aagagtattt
      421 caaatcttaa aataacgcct actcttgata aaatactaga ggcaaatgac acaagatttg
      481 gtttgattat tatctgcaca ggctttacca gactaaaagg aaactatggt aaagaaattg
      541 caaaaggagc tgcactaggt atattaactt tagggatgta ttatcaaatt ccagtaaaag
      601 cctattcgac tatatatgct atgattgtcg attcaaaaga agataacgtt gcattttata
      661 gaaagtcatt taagcaagat cttgaaccac tagatcaaga agtcttgaca aatcaatata
      721 agaaaatttt tgaaggatat ttctggacaa ctaaatgatt gccagctgct aacaaacggt
      781 ataggtcatg gctatctttt gtaaccactg gagctttgta aacgaaatta aactttgtat
      841 accacgacag gaaaatgtct tttagtcgcc acgacccata ccgtcaactg ttgacattca
      901 ttgtaaaagt aacctgctaa ttataaaaat catcatagat taaaatgatt aacatgaaac
      961 gaaatgtaac taggaaaata attggtggat taagtttgac atctgcaatg tttgtatttc
     1021 aagcatgcta tggtacacct caggattttg ggctggatat attaattgaa ggtcaagtaa
     1081 aatcaaagac atcaggtttg ccaattaaag gaataaaagt ttcagttgcc gacaatgtac
     1141 aatatgaggt gactgatgaa gaaggcaagt tttcatttta tacagaaatt cttgaaggat
     1201 taacactaca ttttcaagat attgattcca ctcaaaacgg actttatatt gataaggata
     1261 ctgtattgac agatttaaaa gagaatattt acttagatat agctttggaa gaaaaataaa
     1321 tgctttcatc aatcaaaaaa tacagtttca gaaaatttag agattcagag acaaaacggc
     1381 acgaactgaa ttatttgttt tgggagtgca ctacacgatg taatttaaat tgcctgcatt
     1441 gtggaagtga ttgctcaaaa gatagtttgc atcgagatat gcctgctgag gatttctttc
     1501 aggcaattga cacaattgaa aatataccac aaaactttac ggttgtattt acaggcggtg
     1561 aacctctttt acgaaaggac atagaacttt gtggaaaaga actacgaaaa cgaggtttta
     1621 aatggtcgat agtttcaaat ggacatttat acgataaaca aagacacatt tcattactaa
     1681 atgctggaat aggtgcattg acaattagtt tagacgggct taaggaatct cataattggt
     1741 tgagaaataa tgaaaagagt ttcgataaag taataaatgc aattgaattg gcatcatctt
     1801 ccaatcgtat caacttcgat attgttactt gtgttaacca aaagaatatc aatgaacttg
     1861 aacaaatcag ggaattattg atttcgaaaa atgttaaggc atggagatta tttacaatta
     1921 ttcctatcgg tagagcggct cataatcccg atttgtcgtt agctgacaat cagtttgtcc
     1981 aattaatgga ttttatttca gaaactcgaa aatccaaagg aattgacgtt aagtttagtt
     2041 gtgaaggcta tgttggaaaa tacgaattat tagtaaggga ttcgtacttc ttttgcagag
     2101 caggaattaa tattggctcg attcttattg acggttcaat ttcagcctgt cccaatattg
     2161 acaggtcttt ttcgcaagga aatatttaca aggacaattt ttataaggtt tgggaaaacc
     2221 aatttcaacc atttcgaaac agagaatgga caagaatcgg tcaatgtgaa ttgtgcaaag
     2281 attttaaaga ttgccaggga aatggatttc ataactggca tggagataag aaaaacgtat
     2341 tagtctgtca taatgagaaa atagaaaaac aacgaaatgc caacattttg tataagtaat
     2401 ggcaggagtt gtatcaagta tcaaaggttg taacccgctc aaactgcgta gcggtttgac
     2461 agtgaagaag cccgcaatcc ccaacgtttc atacacgcaa ccgttgtgcg gtcatttcag
     2521 gacgaccccg ccgctaagga taatgtgaat aaaaacacgg caccctatgg cttcaaatcc
     2581 atggtataag ttagatttcc cccgctaccc tgcgagcccc ctcgtgaggt gaataactaa
     2641 taatggtaaa gtgcagaagg agtagcctat tggtgcttta cctgctgtta aagtgtattt
     2701 gaaaggtcgc cgaaaggtaa aaccgattcg cccgccggcg gagagcttgg cgaaggcaac
     2761 catgcggcag caaaggcgtg tattttttta ttcacacacc gaaccaaacg aagtgaattg
     2821 aggtgaaaac cttaatttta taggtccgtt tacatgtata aaatttatgt acatttgctt
     2881 tcaaattaac cccttaccag atgtttagaa tttcatcgtt aacaagtgct ttatttttta
     2941 gcttggctat cctagctgca tgtgtgcctg ccaaggaatt tcaagcgcta caggataaga
     3001 atgccaagtg tgaggaggag cgcgaaaagc tgagtaccga aaacgaggat ttaagctccc
     3061 gatcggccga acttcaggca aagaatattt tgctggaggc aaaggtaagc acgctggcct
     3121 ccgatagcgc aatatgcgcc gttgagctga gcaaaacaag ggagcagctg gcaaccctta
     3181 acgagcgcta cgccgacctg cagagtattc aggatggggt aatctccgga agtaaggatg
     3241 aaacaagaaa gctgctggct caaattcaga agaaccagga ggagcttcag cagaaggagg
     3301 atgccctgaa ggagctcgag cgaaagcttt accagcgcaa gcttggtctc gaccaggttc
     3361 aggcaaatct cgacaagatg agcaaggacc ttgaggaaag aaacgcccgt ctggtcgagc
     3421 tcgaacgaat gcttaacagc aaggactccc taatgaacgc tcttaggaaa aaggttgccg
     3481 atgcgctttt tgggtttgaa ggcaaggggc taactgtttc cattcaaaac ggcaaggttt
     3541 acgtttcgct ggatgagaag ctgatgttca aatcggggcg ttacgagata gatgccaagg
     3601 gggctgctgc cataaaacag ctggtgccgg tgctcgagca gaacccggat atcaacatta
     3661 tggttgaagg tcataccgac gatgttccct accggggaac gggcgacctg atggataact
     3721 gggatttgag cgttaaacgc gctacaacca ttgttaggtt gctgctcaat ggaagcaaaa
     3781 ttgatcctgt tcgcgtaacc gctgccggac gtagccattt tttacctgtt gatagggcta
     3841 aaactcctga ggccaggcag aaaaaccgcc gaactgaaat catccttaca ccaaaaatgg
     3901 atgaaatatt aaagctactc gaggccaact agacaaaagt tgcctacact ccgttcaacc
     3961 ctgaggttat aggaggtttt tcggcctgtt tttgcataat cgggcaattg ccataaattt
     4021 gtacccatta aatcaccgtt tatgggttta cagtgtggaa ttattgggat aaccaatgtg
     4081 gggaaaacca ccattttcaa ctgcatgtcg agcaccaagg cggctgccag cagttttgcc
     4141 tttagtgcca gcaagtcgaa cataagcatc attaatgttc ccgatagccg gctctacgag
     4201 ctggaaaagt ttcagcccac cgagcgcatt gttcacacta cagttgagat tgttgatata
     4261 cccgggttag ccaaggggtc caacaagggc gaaggagttg gcaacaagtt cctggccgat
     4321 attcgcaact gtgatgccct catacatgtt ctacgttgct tcgacgaccc caacctgccc
     4381 cacgttgatg ggtcggttga cccagttagg gatattgaaa cggtgaactt tgagctgcag
     4441 gtgcgcgatc tggagtcaat tgagaagaag cttcagcgcc tcgaaaaggc tgccaaggtt
     4501 ggcgataagg aggccaaaca ggctgctgcc gtgcttggcg tgtacaagaa ccacctggaa
     4561 tcattccaga atgctgctac ggcgcctgtt gacgaacagg acaaaaagta tgttgaagac
     4621 cttttcttgc tgtcaactaa acctgtcata tacgtttgca acgtagatga ccaatcggca
     4681 gtaacaggta accactatgt tgacaaggtg agggaattcc ttaaaaacga gcaaaccgaa
     4741 atactggtta tagccggtaa gctggaagct gaaattgccg aactcgagaa tgaggatgat
     4801 cggttggcct tcctttccga tgtgggactt accgaacccg gggtaaacaa gcttatcagg
     4861 gccgcgtact ccctgctcaa catggagaca ttctttaccg ttggaccaaa ggaaattcgt
     4921 gcatggacca tcaagaaggg aatgacggct cctcaggctg caggggtaat tcattccgac
     4981 ctggagcgcg ggttcattcg tgctgaggtt atgaagtata acgatttcat aaccctcggt
     5041 tccgagcagg cctgcaagga gaagggtaaa ctttttgttg agggcaagaa ctacgttgtg
     5101 gaggatggcg acattctgca tataaggttt aacgtgtagc agccggctac attgctgagc
     5161 aatatttttc ctgaatcttc ttattaatct ggtgctaaac catcatactt tcaatgatgt
     5221 tttagattgt ccctgtttcc tcgaaatgta gtttattgcc gcataaatct tccattcgac
     5281 aggtaaattt tctgttcact tttcaaaaca attacctcct aagctaaagt aaaggttgaa
     5341 ttacaccatg cttttgcgcc ggtctttttg gcctggccct tatttgggat gtcggacaaa
     5401 cactactaca ctaccggcaa gatgcaagtt cttgcacacg ccgggttggg atgaagtggt
     5461 agcgaatggc aggggtgtta ctggtggctg caaatctagg agaagccata agcaagggca
     5521 gcgtgcagac gcacacaaat ccgccggtag gcggacaagt ttttgaatcc tgcttgaatt
     5581 tttttatcct ttatcct
//
LOCUS       DAOPHX010000144         5534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_19422_length_5534_cov_6.188721, whole genome shotgun sequence.
ACCESSION   DAOPHX010000144 DAOPHX010000000
VERSION     DAOPHX010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19422_length_5534_cov_6.188721"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..736)
                     /locus_tag="PK502_09385"
     CDS             complement(20..736)
                     /locus_tag="PK502_09385"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HPH01069.1"
                     /translation="MSTSDKNDILSVLYEAIPAGNKQGEDIFTFGSVYVAASTSEGNI
                     GVCATLGVTVPCFNPAYFNIDRVADRVALNALINARVNYSVLYDGAADIFDVVDFTRY
                     QSIVMIGYFRSLVDKLSSSGVETFIFDLNQHDVPVMPIKLQQEYLGRADCVIVSSTSI
                     SNGTFNGLIVNTPASTNVYMLGPSTPMCDVMFSYPQVKGLFGSVFEPHDQKTIQIIAA
                     GGGTRQFKTCMRKVYRLRHE"
     gene            972..1691
                     /locus_tag="PK502_09390"
     CDS             972..1691
                     /locus_tag="PK502_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPH01070.1"
                     /translation="MDESKVIEISNANIFQEDNLILSDVNLSLAQGEYLYIIGRVGSG
                     KTSLIKTITAELPLKSGEATVCGFNLRTIKRRHIPKLRRKIGVVFQDFQLLSDRSVYD
                     NLLFVLRSTGWKDKQAIKQRITEVLEMVGLKFKDYKLPHQLSGGEQQRVVIARALLNN
                     PEIILADEPSGNLDMESSEGIMQILHSIAESGKSVVMATHNHQLIKKFPARTLKCEKG
                     KLLEIEQMGEIDFNSLMDMDG"
     gene            2080..3057
                     /locus_tag="PK502_09395"
     CDS             2080..3057
                     /locus_tag="PK502_09395"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPH01071.1"
                     /translation="MIIVQKPEQTLGKEVALTMGFFDGVHAGHRTLIERVLQVSGLRN
                     LGSAVLTFWPHPRLVLQKDPEKLRFLTTLTEKSKIVSKLGVDFLIIKEFTPDFYNMEA
                     EDFIRHLVKYYHVKHFVVGSKHRFGKGGRGDTALLHELSAELSYTLEVVETLQVDGQG
                     ISSTKIREALLSGNVETANQMLGYPYLITGSVESGKKVGREIGFPTANIRPNDPLKLI
                     PREGVYAVVVSIEGSIRFGMMNIGFRPTVDTMRKQTIEVNIFDFDDEIYFSGIEVAII
                     ARLRNEKRFPSIKELSEQLAIDRIDALNILQTLNPEVFQNLFLTLRPSK"
     gene            3081..4496
                     /gene="ahcY"
                     /locus_tag="PK502_09400"
     CDS             3081..4496
                     /gene="ahcY"
                     /locus_tag="PK502_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPH01072.1"
                     /translation="MEQHGNLQPYKVKDIGLAEWGRKEIEIAEKEMPGLMSLRQKYGS
                     QKPLNGARITGSLHMTIQTAVLIETLVELGAQVRWASCNIFSTQDHAAAAIAAAGIPV
                     FAWKGETLEDYWWCTAQALSFPNGKGPNMIVDDGGDATLLVHWGYKAENDRKFVERKA
                     QNHEEEVILNLIRETLQQEPNKWHNLAAELKGVSEETTTGVHRLYQMKEKNELLIPAI
                     NVNDSVTKSKFDNLYGCRESLADGIKRATDVMIAGKVVVVCGYGDVGKGCARSMRSYG
                     ARVIVTEIDPICALQAAMEGFEVKTVDDALAEGNIFVTATGNCDIITLEHMQQMKDQT
                     IVCNIGHFDNEIQMDRLNTQKGVRKVNIKPQVDQYYFPDGHSIFILAEGRLVNLGCAT
                     GHPSFVMSNSFTNQTLAQVELWTKHLAVDVYRLPKQLDEEVARLHLEQLGVKLTTLTP
                     KQADYIGVNPLGPYKSDHYRY"
     gene            complement(4532..5464)
                     /gene="queG"
                     /locus_tag="PK502_09405"
     CDS             complement(4532..5464)
                     /gene="queG"
                     /locus_tag="PK502_09405"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HPH01073.1"
                     /translation="MAMIPLEIVRKLALDVGFSDCGVSPAGKLGDAGNGLLSWLGKGY
                     HGEMAYMERNLNLRLNPSALVPGAKSVISVILAYGHQEIPLMAHPPRVSRYALMPDYH
                     KVMKTMLFRLLNALREEFGPVNGRPFVDSAPVLERAWAENSGMGWIGKNSNFIHHKFG
                     SFVFIGELIVDAAVEDSSRQHKNRCGSCTRCMEACPTHAIVEPGVVDARLCISYLTIE
                     KRSPLTEKECGSLNGWCFGCDICQEVCPWNQKLLPITSEEVSIIPELKQLNAGNMMGM
                     TQTQFEEWFGITPLKRAGYEKLMQSVSCAVKASH"
BASE COUNT         1577 a         1299 c         1270 g         1388 t
ORIGIN      
        1 gtaaaataat ctggctggct tactcatgcc tcaacctgta aaccttcctc atgcaggttt
       61 tgaactggcg agtgccgcct ccggcagcaa taatttgaat ggttttttgg tcatggggct
      121 caaaaaccga cccaaaaagc cccttaacct gagggtagct gaacattaca tcgcacatgg
      181 gtgttgaagg ccccagcatg taaacattgg tgcttgctgg cgtattgacg atgaggccgt
      241 taaaagttcc gttagaaatg ctggttgagc tgactatcac gcaatcggcc ctacccaggt
      301 actcctgctg cagctttatt ggcattacag gtacatcgtg ttggttcagg tcgaaaataa
      361 atgtttcgac cccgctgctg ctgagcttgt caaccagcga cctgaaatac ccaatcatca
      421 caatgctttg gtaccttgta aaatctacaa catcgaagat atctgctgcg ccatcatata
      481 aaaccgaata gtttaccctg gcgttaatca gtgcgttcag cgctacccgg tcggctaccc
      541 ggtcaatatt aaaataggca gggttaaagc agggtacggt aacacccaac gtggcgcata
      601 caccaatgtt cccctcgctt gttgaagccg ccacataaac cgatccaaag gtaaaaatgt
      661 cttctccctg cttgttgcca gcaggtatag cctcataaag aacagacagg atatcatttt
      721 tgtcactcgt gctcatgcca tatttttatt taccgaaatt actgtttgta ccaaacgcca
      781 aaagtagctg aaaaattcac atcccccatt ctgcccaagg tagaggggta gttaagtact
      841 acctacaact ttatccatgg cactcctttc atctaaacag taaaaaatac aaagccaacc
      901 cttagatgtg gtatcctagc gtttaaataa atattatacc ttagcaacaa attttgcgct
      961 taacccatac catggacgaa agcaaggtaa tagaaattag caatgccaac atcttccagg
     1021 aggataacct catactttcc gatgttaacc tgtcactggc tcagggcgaa tatctctaca
     1081 tcatcggtag ggttggcagc ggaaaaacca gcctaataaa aaccataacg gctgaactcc
     1141 cccttaaatc gggcgaggca acagtttgtg gattcaacct tcgaacaatc aaacgcaggc
     1201 atatccccaa gcttcgccgt aaaattgggg ttgtgtttca ggattttcag ctgctatctg
     1261 atcggagcgt ttatgacaac cttctgtttg tgctaaggtc aaccggctgg aaggataagc
     1321 aggccatcaa gcagcgaata accgaggtgc ttgaaatggt tgggctaaag tttaaggact
     1381 acaagttgcc ccatcagctt tctgggggtg agcagcagcg ggtagtcatt gcccgtgcgc
     1441 ttctcaacaa ccccgaaata attctggccg acgagccctc gggtaacctg gacatggaat
     1501 cgtccgaagg gattatgcag atactccata gcattgccga aagtggaaag tcagttgtaa
     1561 tggccaccca taaccaccag ctgattaaaa agtttcctgc ccgcactttg aagtgtgaaa
     1621 agggaaaact cctcgaaatc gagcagatgg gagaaattga cttcaacagc cttatggaca
     1681 tggatggata accctacgct atttgctggt tttactccat ttatgaggat tttttcacca
     1741 tattttagca tcaacccaac ctgttcatga gtaagggtta aaaagttttg atggattcca
     1801 ggcaaaacac taaatgagcc gcagtctcta accttcacaa ggctcacttc tctaaaagag
     1861 atgtttcatg tgataaattc tttacaagca gaaaaatatt tgcaccttgc caaaaggcat
     1921 gcttttcttt tggtgcaaat taaccattgc ccacatccaa ataatcgctg gtcaacaatt
     1981 ttaaaacgtt ggtcaatgca gaattaatac agtcttaggt aaagttgtaa ttaaagtcat
     2041 aaatttgcac cttagcatcc tgtattaacc agacctgcga tgataatcgt tcaaaagccc
     2101 gaacaaaccc ttggaaagga ggtggcctta accatgggct tttttgatgg cgttcatgcc
     2161 ggtcatagaa ccctcataga aagggtgtta caggtctcgg ggttgagaaa tttaggttca
     2221 gccgttctta ccttttggcc acacccccgg ctggtgctcc agaaagaccc tgaaaagcta
     2281 agatttctta ccacccttac cgagaaatcg aaaattgttt caaagctggg agttgacttt
     2341 cttatcatta aagaatttac acccgatttc tacaacatgg aggctgagga cttcattcgg
     2401 cacctggtta agtattacca tgttaaacat ttcgtggtgg gctccaaaca tagatttgga
     2461 aaaggtggaa ggggagatac agcgttactg catgagcttt ccgcagagtt gtcctatacc
     2521 ttagaagtag ttgaaacgtt gcaagttgat ggtcaaggta ttagctcaac aaaaatcagg
     2581 gaagccttgc tttcgggcaa cgttgaaacg gccaaccaaa tgctgggata tccctaccta
     2641 ataaccggct ctgttgaatc gggcaaaaaa gttggcaggg aaataggctt tccaacggcc
     2701 aacattcgcc ccaacgatcc cctaaaactc atccccaggg agggagtata tgctgtggtg
     2761 gtcagcatcg agggctccat caggttcggg atgatgaaca tcgggttcag acctaccgtt
     2821 gacaccatgc gtaagcaaac catcgaggta aacatctttg attttgacga cgaaatatac
     2881 ttttccggaa tagaagttgc cataatcgca aggctaagga atgaaaagcg ttttccctcc
     2941 ataaaagagt tgagcgagca gctggctatt gatagaatag acgccctgaa tatacttcaa
     3001 acactaaatc cggaagtttt tcagaattta ttcctaactt tgcgcccctc aaagtagagt
     3061 aaagtaaata caagcacaaa atggaacagc atggaaattt gcaaccctat aaggttaagg
     3121 atattggatt ggccgaatgg ggaaggaagg agatagaaat tgcagagaaa gaaatgccgg
     3181 gtttaatgtc gctgaggcag aagtatggct cccaaaagcc acttaacggg gcaagaataa
     3241 ccggttcgct gcacatgacc attcaaacag cagtactcat cgaaactttg gttgaacttg
     3301 gcgctcaggt tcgctgggct agctgcaaca tattttccac ccaggatcat gccgctgctg
     3361 caatagccgc agcaggtatt ccagttttcg catggaaggg cgaaaccctc gaagattact
     3421 ggtggtgcac tgctcaggct ctatctttcc ctaacggtaa gggtcctaac atgattgttg
     3481 atgatggtgg cgatgctacg cttttggtgc actggggcta caaggccgag aacgatagaa
     3541 aattcgtgga gcgtaaagcc caaaatcatg aggaggaggt tattcttaac ctgattaggg
     3601 aaacccttca acaggaacca aacaaatggc acaaccttgc cgcggagcta aaaggagttt
     3661 ccgaggaaac cacaacggga gttcaccggc tttaccagat gaaggagaaa aacgagctgc
     3721 tgatacccgc catcaacgtg aacgattctg ttaccaagag caagttcgac aacctatacg
     3781 gctgccgcga gtcgcttgcc gacggcatta aacgagctac cgacgtgatg attgccggaa
     3841 aggtagttgt ggtttgcggt tacggtgatg tgggtaaggg atgcgcccgc agcatgcgct
     3901 cctatggcgc cagggtaatt gttaccgaaa tagatccaat atgcgccctg caagccgcaa
     3961 tggagggatt tgaggtgaaa acagttgacg atgcactagc cgagggtaac atctttgtta
     4021 ctgctacggg caactgcgat attattaccc ttgagcacat gcagcaaatg aaagatcaga
     4081 ctattgtgtg caacattggc cacttcgata acgaaattca gatggatagg ttaaataccc
     4141 aaaagggtgt tagaaaagtc aacataaagc ctcaggttga ccagtactac ttccctgatg
     4201 gtcattccat attcatactt gccgagggtc gactggttaa cctgggctgc gcaacaggtc
     4261 atcccagctt tgtgatgagc aacagcttta ccaaccagac tttggcacaa gttgaactat
     4321 ggacaaagca tttagctgtt gatgtttacc gccttcccaa gcagctggac gaggaggttg
     4381 ccaggttaca cctggagcag ctcggggtta agctcacaac gcttacgccc aaacaggccg
     4441 attacattgg tgtaaatcct ctcggcccat acaaatccga tcactaccgt tactaggata
     4501 gctaccgaaa tgccggcagt tgccggcatt tttaatgtga agctttcacc gcacagctaa
     4561 ccgactgcat taacttttcg tatccagccc tttttaacgg ggtaatgcca aaccactctt
     4621 caaactgtgt ttgtgtcatg cccatcatgt tgcctgcgtt aagctgtttc aactctggga
     4681 taatcgagac ttcttcgctc gttataggga gtagtttttg gttccagggg caaacctcct
     4741 gacaaatatc gcagccaaaa caccagccgt tcaggcttcc acactccttt tccgtaaggg
     4801 ggctcctctt ttcaatggta aggtacgaaa tgcaaagtcg ggcatctaca acgccaggct
     4861 ctacaatggc gtgtgtggga catgcctcca tgcaccgggt gcagctgccg catcgatttt
     4921 tatgctgacg gctggaatct tcaactgctg catcaacaat gagctcacca ataaacacga
     4981 acgagccaaa tttgtgatgt atgaagttgg agttcttgcc tatccaaccc atgccagagt
     5041 tttcagccca tgcacgctcg agcactggtg cagagtcaac aaagggcctt ccgtttactg
     5101 ggccaaactc ctcccttaag gcattgagaa gtctaaatag catggttttc attaccttat
     5161 ggtaatcggg cataagtgca tagcgggaaa ctcttggtgg gtgggccata aggggtattt
     5221 cctgatgacc ataggcaaga atcacgctga ttaccgattt tgcccccggt accaatgccg
     5281 aagggttaag acgaaggttg aggtttcgct ccatgtaggc catttcgcca tgatacccct
     5341 ttcccaacca gcttagcaac ccattgccgg catcgccaag ctttccggca ggagatacgc
     5401 cgcaatcgct aaaacccaca tctaaagcca gtttcctgac tatttccaaa ggtatcatcg
     5461 ccatcaaaac tcctttgacc gatataatgg gttcaaagat agcataagga tgataataag
     5521 gttaaggata aagg
//
LOCUS       DAOPHX010000145         5498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_19597_length_5498_cov_6.838876, whole genome shotgun sequence.
ACCESSION   DAOPHX010000145 DAOPHX010000000
VERSION     DAOPHX010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19597_length_5498_cov_6.838876"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="PK502_09410"
     CDS             complement(<1..1054)
                     /locus_tag="PK502_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPH01074.1"
                     /translation="MSKNNLLIASAMLLFATLTAQGQEPSNPFFSVYSTPFQVPPFNL
                     IAIEHYAPAFERGIEEQKKEIEAITSNPQAPNFENTILAFDKSGELLTKVSSVFYSLN
                     SANTSPEMQKVARVISPLVTAHRDNINLNDKLFQRIKAVYTKRDSLKLDSLQKRTVEK
                     YYNDFVRNGANLDEAAKARLREINQQLSELGLHFGENLLAETNKNFKLVIDNPADLDG
                     LPKDIVDAAAITAKEFGLEGKWVFTLQKPSMIPFLQYAKNRNLREKLYTGYAMRCNNN
                     DEFDNKSIMLQIVNLRAEKAKLLGYKTYADYSISNNMAKTPEAVYDFLNQVMEPALIA
                     ATRDRDDMQAIINREGG"
     gene            complement(1154..3697)
                     /locus_tag="PK502_09415"
     CDS             complement(1154..3697)
                     /locus_tag="PK502_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HPH01075.1"
                     /translation="MRTLSKYKIIPENAMKRNLLTVSLMVLVNTIIFGQNIPIEKANY
                     ELPARFSPKKVEKMVFSTTVQPHWLKNSTRFWYSYETPSGKSYIIVDPAKRSKGFLFD
                     NNNMAAQLSLLTKEPFDAQHLPIQKIKFIKNETAIQFEVKSTLVDEDKSDEDEENDMV
                     KKKEGKAKAKPDKKTYYFEYDLATRKLMLLEDYKKPKENPEWANISPNGEMVVFSRHH
                     NLYWMDKENYEKAKKNEDDSTIIEHQLTTDGEKYYSYGYTRENEDNVDEEKNKDKRKP
                     TYITWSFDSKLFAMTRTDMRNVKELWLVKTLSSPRPTLETYKYHMPGEKEAPKDELLI
                     FNLAQDTIIKPDVAAFKDQDLMILNARVKANERDDDYVATRWLSKYNDRLYFVRTSRD
                     LKRVDVCYVDTKTGKVNVVIEERLNTYIDFNGISLINNEKEIIHWSERDGWGHFYLYD
                     NNGKFIRQITSGPFHCENLVGYDETTKTLFFTANGCEPNEDPYYLHLYRVGLDGNGMK
                     LINPGNFDHYISMDDGNRYFVDNYSRVNTNPETRLLDAQGNELLALEKADLSLLFQAG
                     YKFPEIFKVKAADGITDLFGVMYKPFDFDSTKLYPLIEYVYPGPQTESVNKSFSARLD
                     RTDRLAQFGFIVITVGNRGGHPARSKWYHNYGYGNLRNYGLADKKAAAEQLAYRHNYI
                     DINRVGIHGHSGGGFMTAAALLTYPDFFKVGVSNAGNHENNIYNRWWSEKHHGVKEIT
                     DTSGKVSFEYSIDKNSEIAKNLKGHLLLTTGETDNNVHPGNTIRLANALIKANKRFDL
                     FMFPGQRHGYGDMTEYYFWLMGDYFSKYLIGDYSTSVDITPMNNEMEKR"
     gene            3910..>5498
                     /locus_tag="PK502_09420"
     CDS             3910..>5498
                     /locus_tag="PK502_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPH01076.1"
                     /translation="MKRLLCIFILAALPAVAYLQPPLLTVAESSNFTSTSTHSEVMAY
                     VERLQKTSKFLRVEKIATTTEGKDLPLLVIGNPVPKDPKDLHGRLVVYIQANIHAGEV
                     EGKEASLMFARDLVAKRDPELLKHLVVLICPDLNADGNDRISKLNRTYQNGPANGVGE
                     RQNGQWLDLNRDAMKLESPEMRGVVANVLNRWDPAIIVDCHTTNGSYHQEPVTFTWMM
                     NPNGDRELIRYMRDRMMPWVSQTLSGKYGTLNCFYGEFINQQNYEEGWISYASEPRYF
                     SNYVGIRNRLGILNENYVYAGYRERVLGCYNLIASITDYALAYQTEIMQMLARADSIS
                     LAIGSQAASSDSFAVKYSGTPTPQKITILTYEAEAYTDEHGRERFRKTDTKRTVTVPY
                     IADYIPVKSIAYPYAYILPVPDPNVLQLLRQHGVEFQMLTDSVTLTVEQFAIDSIAPD
                     RRLNQGHYCNTINGSYQKVSRRFNKGDMVVKTSQKLGRLAAYLLEPQSDDGLVYWNFW
                     DRYLVPQWGSYFFPVPVYRLT"
BASE COUNT         1272 a         1281 c         1327 g         1618 t
ORIGIN      
        1 taccgccctc ccggttgatg atggcctgca tgtcatccct atcgcgggtg gccgctatca
       61 gtgcaggttc catcacctgg tttaaaaaat cataaacagc ttcaggtgtt ttggccatgt
      121 tgttgctgat actgtagtcg gcataagttt tgtatcccaa cagtttggcc ttttcagccc
      181 gcagatttac gatttgaagc ataatgctct tattatcaaa ctcatcgtta ttgttgcacc
      241 tcatggcgta cccggtgtag agtttttccc taaggtttcg atttttggcg tactgcagga
      301 agggtatcat gctgggtttc tgaagggtga atacccattt accctctaat ccaaattcct
      361 tggcggttat ggctgccgca tcaacaatgt cctttggtag gccatcgaga tcggcaggat
      421 tgtcgattac cagcttaaaa tttttattgg tttcggccag caggttttcg ccaaagtgga
      481 gtcccagttc tgaaagctgt tggtttatct ccctgagcct tgcctttgct gcctcatcga
      541 ggttggcacc gttgcgaaca aaatcgttgt agtatttttc aaccgttctt ttttggagcg
      601 aatccagctt taggctatcc ctcttcgtgt aaaccgcctt tatccgttgg aataacttat
      661 cgttaaggtt gatattgtcg cgatgggctg ttaccagagg tgaaattact cttgccacct
      721 tttgcatttc gggtgatgtg ttagcgctgt taaggctgta gaaaacgctg cttaccttgg
      781 ttagcagttc tcccgactta tcaaatgcaa gaatggtatt ctcaaagttg ggagcttggg
      841 gattgctggt tatggcttca atctcctttt tctgctcttc aattcccctt tcaaatgccg
      901 gggcatagtg ctcaattgca atcaggttaa acggtggaac ctgaaatggt gtggagtaca
      961 cgctgaaaaa cgggtttgac ggctcttgcc cctgtgcggt tagggttgcg aatagcagca
     1021 tggccgaggc gattagtagg ttgtttttgc tcatttctgt taaaattaaa attataattt
     1081 tttttactag gatgttttcc ttttgtcagt agtttaaaca tgaaaaggga tggctaaacc
     1141 ttaaggcttg gctttaccgt ttttccatct cgttgttcat gggcgttata tccacgctgg
     1201 ttgagtagtc tccaatgagg tatttgctga agtagtcgcc cattagccag aagtagtact
     1261 cggtcatatc gccgtagcca tgcctttggc cgggaaacat gaataagtcg aaacgcttgt
     1321 ttgccttaat cagcgcattg gccagcctga tggtgttacc cggatgcacg ttgttgtcag
     1381 tttctccggt ggtaagcagc aggtgtccct tcaggttctt cgcaatttcc gaattcttgt
     1441 caatgctgta ctcaaatgac acttttccgc ttgtatcggt tatttcctta accccgtggt
     1501 gtttctcgct ccaccagcgg ttataaatgt tgttttcgtg gttgcccgca ttggacaccc
     1561 caaccttgaa gaaatcgggg taggtaagga gggcagcggc ggtcatgaag cctcctcccg
     1621 aatggccatg tattcccacc cggttaatat caatgtagtt gtggcggtag gctagctgct
     1681 cggcagcagc ctttttatcg gcaagcccgt agttacgaag gttgccatag ccatagttgt
     1741 ggtaccactt ggagcgtgca ggatggccgc ccctgttacc aaccgtaatc actataaacc
     1801 caaactgcgc caacctgtcg gttctgtcta ggcgagcaga aaacgatttg ttaaccgatt
     1861 cggtttgcgg tcccgggtaa acatactcaa tcagcgggta gagcttggtg gagtcaaaat
     1921 caaatggttt gtacatcacc ccgaacaggt cggtaatgcc atcggcagcc tttaccttga
     1981 atatttcggg gaatttgtac ccagcctgga agagtaagct taaatcggct ttttccagtg
     2041 caagcagctc gttgccctgg gcatcgagta gcctggtttc gggattggtg ttgacccggg
     2101 agtagttgtc aacaaaatag cgattgccat catccatgct gatgtagtgg tcaaaatttc
     2161 ctgggttgat gagcttcatt ccgttcccgt caagtccaac tctgtacagg tgcaggtagt
     2221 aggggtcttc gttgggttcg caaccgttgg ctgtgaaaaa caacgttttg gtggtttcat
     2281 cgtaacccac caaattctcg cagtggaagg gtccggaggt aatctgtcgg atgaatttgc
     2341 cgttgttgtc gtagaggtag aagtgccccc atccgtcgcg ctccgaccag tgaataattt
     2401 ccttctcgtt gttaatcagg cttataccat taaaatctat ataggtgttg agcctctcct
     2461 cgattacaac atttaccttg ccggttttgg tgtcaacgta gcaaacgtca acgcgcttta
     2521 ggtcccttga cgtgcgaaca aagtagagcc tatcattata cttcgacagc cacctggtgg
     2581 caacatagtc gtcgtcgcgt tcgttagcct ttacccgggc attcagaatc attaaatcct
     2641 gatccttaaa tgcggcaaca tcgggtttga tgatggtgtc ctgagccaaa ttgaatatca
     2701 acagctcatc ctttggtgct tctttctcac ctggcatgtg gtacttgtag gtttccagcg
     2761 tgggccgtgg cgacgatagc gttttaacca gccatagctc ctttacattt cgcatgtcgg
     2821 ttctggtcat ggcaaacagc ttggaatcga acgaccatgt aatgtaggtg ggcttgcgct
     2881 tatccttatt tttctcctca tccacgttgt cctcgttttc cctggtatag ccgtagctgt
     2941 agtacttttc gccatcggtg gtaagctggt gctctataat ggtggagtcg tcctcgttct
     3001 tttttgcctt ctcatagttc tccttatcca tccagtatag gttgtgatgt ctgctgaaca
     3061 ccaccatctc accgttgggc gaaatattag cccactccgg attctccttg ggttttttgt
     3121 agtcctctaa tagcattagc tttcgggtag ccaggtcata ctcaaagtag tatgtcttct
     3181 tatcgggctt tgctttggct ttaccctcct ttttcttaac catgtcattc tcctcatcct
     3241 catccgattt gtcctcatcc accagcgtgc ttttcacctc aaactggata gctgtttcat
     3301 tcttgataaa cttaattttc tgtattggaa gatgttgggc atcaaatggt tccttagtca
     3361 gcagcgagag ctgtgcggcc atgttgttgt tatcaaagag aaaacccttg ctacgttttg
     3421 ccgggtcaac aataatgtac gattttccgc ttggcgtttc gtagctgtac caaaagcgtg
     3481 tgctgttctt cagccagtgt ggctgaacag ttgtgctgaa aaccattttc tctacttttt
     3541 ttggcgagaa gcgggcgggt agctcgtagt tggccttttc tattgggata ttttgcccaa
     3601 aaattatggt gttgacaagc accatcaatg atacggtaag caggtttctt ttcatggcat
     3661 tttccggaat aattttatat tttgataaag ttctcattac atggtttagc tgttaaacca
     3721 ttaaaaaggc aaacggtcaa aagccgtgtt cctgtgttta aaaaccgggt aaccaaaggg
     3781 atgcagtttc aaaacatgta aactcacctg ctacattttt aaatgcttgg atggaatgat
     3841 ttttaaatat ctcaaaattc attaaagttt attttttctg gacatagcta acaataatat
     3901 taggaactga tgaaaaggtt actttgcatt tttatactag ccgctctgcc agctgtggct
     3961 tacctacaac caccgctact aactgttgcc gagagcagca actttacctc aacttctacc
     4021 cacagcgagg tgatggcata tgttgaaagg ctgcagaaaa cgtccaaatt tttacgggtg
     4081 gaaaaaattg ccacaacaac cgagggtaag gacctgccgc tgctagtaat tggaaaccct
     4141 gtgccgaaag atcctaagga tttgcatggc cgtttggtgg tgtacattca ggccaacatt
     4201 cacgctggag aggttgaagg caaggaagcc tcgctgatgt ttgcccgcga cctggttgcc
     4261 aagcgcgacc cggagctgct gaaacattta gtggtgctaa tttgtccgga tttgaatgcc
     4321 gatggtaacg accgaataag caagttgaac agaacgtacc agaatgggcc tgctaacggg
     4381 gtaggcgaga ggcagaacgg tcaatggctc gacctgaatc gtgatgctat gaagctggaa
     4441 tcgcccgaaa tgaggggagt tgtagccaac gtgcttaacc gctgggatcc ggctatcatt
     4501 gtggactgcc acaccaccaa tggctcctat caccaggaac ctgttacttt tacatggatg
     4561 atgaatccca acggcgatag ggagcttatt cgctacatgc gcgataggat gatgccctgg
     4621 gtatcgcaaa ccctaagtgg caagtacggc acgctgaact gcttctacgg tgagttcatt
     4681 aaccagcaaa actacgagga aggttggatt tcttatgcct ctgaaccccg ctacttctcc
     4741 aactacgttg gaataaggaa tcgcctggga atacttaacg agaattatgt ttatgccggt
     4801 tatcgggagc gcgtgctggg gtgctacaac ctgattgcct ccataaccga ttatgctctg
     4861 gcataccaaa cggaaatcat gcaaatgctg gcaagggccg acagcatatc gcttgctatt
     4921 ggctcacaag cggccagcag cgattcgttt gcagtgaagt actcgggcac ccccacgccc
     4981 cagaagatca ccattctcac ctacgaggca gaggcataca ccgatgagca tggcagggaa
     5041 cgattccgaa aaaccgacac caagcgcacg gttaccgtgc cctacattgc cgattacatt
     5101 ccggttaaaa gtattgccta cccctacgcc tacatcctgc ctgtgccgga cccgaatgtt
     5161 cttcagcttc tccggcagca tggtgttgaa tttcaaatgc ttaccgatag tgttacgttg
     5221 acggttgagc aatttgctat tgactccatt gctcccgaca ggcggttgaa tcaggggcat
     5281 tactgcaaca ccatcaatgg cagctatcaa aaggtgagcc gccgttttaa caagggcgat
     5341 atggtggtaa aaacttcgca aaagctaggt cggttggctg cctacctgct cgaaccccag
     5401 tcggacgacg gattggttta ctggaatttt tgggacaggt acctggttcc ccagtggggt
     5461 agctactttt tcccggtgcc ggtgtaccgc ttaaccag
//
LOCUS       DAOPHX010000146         5404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_20086_length_5404_cov_6.333894, whole genome shotgun sequence.
ACCESSION   DAOPHX010000146 DAOPHX010000000
VERSION     DAOPHX010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20086_length_5404_cov_6.333894"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            461..1093
                     /gene="yihA"
                     /locus_tag="PK502_09425"
     CDS             461..1093
                     /gene="yihA"
                     /locus_tag="PK502_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPH01077.1"
                     /translation="MVIRSAKFVASYPGVDRCPASSIPEYAFIGRSNVGKSSLINMLT
                     GTKTLAKISETPGKTQLINYFLVNESWYLVDLPGYGYAKASKDKRKEFSQIITNYILK
                     RSSLTLLFVLIDSRLEPQPIDIDFINWLGVNQIPLALVFTKTDKTPKALLEANMEAFK
                     NKMMEMWEVLPPIFTSSAVTRTGREDVLSYIDSINANFKSTQPKKVERVW"
     gene            1136..2080
                     /locus_tag="PK502_09430"
     CDS             1136..2080
                     /locus_tag="PK502_09430"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPH01078.1"
                     /translation="MHSKHNIKFFTGRSSRYLSERIADSFGIPLGKSNVTVFSDGEFQ
                     PSFDESVRGCTVFIIQSTFPPVDNLFELLLMVDAAHRASAYKVVAVMPYFGWARQDRK
                     DKPRVAIGAKMVANMLRAAGVDRIMTMDLHADQIQGFFDVPVDHLYASSVFVPYLKSL
                     QIDNIAIASPDMGGAKRANAYARFMNASLVLCYKNREKANQVDEMTIIGDVRGKNVVI
                     LDDMVDTAGTLTKASGLMKDQGALSVRAFATHPVMSGKSYELIRDSQIDQLVVTDTIP
                     LRSDRDTSKIKVLSVAELFADVIEKVYNYKSISEKFII"
     gene            2212..2796
                     /locus_tag="PK502_09435"
     CDS             2212..2796
                     /locus_tag="PK502_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HPH01079.1"
                     /translation="MKTIQLSATLRSETGKKAAKATRKSELIPAIIYGGQKNIMISIG
                     EKDMKKVVYTPDVFVVKLNVDGENFDTIVKEIQFHPVSDAILHVDFYQVSNDKNITIA
                     LPVVLIGQAEGTKQGGKLIQVVRKLKVNGLVKNLPENISIDVTQLELGKSIKVGDLSF
                     KGYTLVDPMSTVIASVKSTRAARETQPTEAAPQQ"
     gene            2808..3371
                     /gene="pth"
                     /locus_tag="PK502_09440"
     CDS             2808..3371
                     /gene="pth"
                     /locus_tag="PK502_09440"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPH01080.1"
                     /translation="MKYLVAGFGNIGPEYSNTRHNVGFMVLDALARASNITFGSARYG
                     DVAELRHKGKTFILLKPSTYMNLSGKAVSYWMQKEKIALENLLVVVDDLALPLGVIRI
                     RSRGSDGGHNGLKSTNETLGTQDYARLRFGIGNGYPRGHQVDYVLGEWSDEELKILPE
                     RLAKAGEAILCFGTIGIERTMNLFNNQ"
     gene            3384..3803
                     /locus_tag="PK502_09445"
     CDS             3384..3803
                     /locus_tag="PK502_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HPH01081.1"
                     /translation="MGAESERVDKWLWMVRVFKTRSLAIDCCRNERVTVNGVPVKASH
                     TVQLNDLVAVRKPPVTLTFRVKGFPGSRIGAKLVDEYAENLTPPEEYQKLDPSFRAFS
                     IYRNPGTGRPTKKERRSLDDFFDSEADDSPWDADDSN"
     gene            complement(3841..5091)
                     /locus_tag="PK502_09450"
     CDS             complement(3841..5091)
                     /locus_tag="PK502_09450"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPH01082.1"
                     /translation="MKKKTTLYIVIGIVVVAVAAHLLLRPRIAQNSYEKGIVLLDSLQ
                     YGQAYEQLNRAVRFSPKNPDYLFGRGMALAGLNRDSAAIADYTKAIAIDSMNFDYFLN
                     RGVSLRKIGNYTASLRDLNRCISLNDSNSRAFYQRGLLNAAMMEYDQAVKDYNRSIEL
                     DGSNLEAFYSRGESLANISDFKGAIADYDRMIEGKNATPESYKKRGTFKLKIQDFNGA
                     ISDLLVALNDLPNDKELLFSLGEAYSNLKDFDQAIGYLDRAASIDPNDPLIYGTRGGA
                     LLNKGDFKAAIQSFNKAINLKPDYLKAVYGRGIAKAFLKDYRGSIEDFNRAISIDKNF
                     TQAYYNRAISKAIMGNHKDALPDFDIAIKYDPKDAEAFFNRGISRMNVKDTKGGCEDF
                     RKALELGYQPAEQMVKIYCSGSGN"
BASE COUNT         1376 a         1236 c         1317 g         1475 t
ORIGIN      
        1 tattccgagc gggatatcag gaaaatctgg ggcgagaatt tcctgcgtgt tttccggcag
       61 gcccgcgatt atgcggcagc tcatgggcag ggcaagtagc tattgctgag ttgctcacaa
      121 tagtaatacc tgcatcacca tagctgaatt ttctccattc atcctgcaat caacacacca
      181 gccagctcat ggctggttta acctttatct aacggcgtat tatgctgatg tggctactac
      241 ccaatgagct atttgcaccg gatataaagc tatccaagca agtttccccg agcgttcacc
      301 tttattctca gcttattatt cccgatgaag gatattttgc tgtgtttttc tatttgtatg
      361 tattttctac tcttttgacc aatggtttgc tttttaattt tataggtttg tatctttgag
      421 gtggattctg ggacaataca tcaaactaaa atttacaact atggtgattc gatctgctaa
      481 atttgtagcg agctaccccg gtgtcgatag gtgccccgct tcgtcaattc ccgagtatgc
      541 atttattggc cggtcaaacg taggaaaatc gtcgctaatc aacatgctga cggggaccaa
      601 aacgctggct aaaatatccg aaaccccggg gaaaacacag ctcatcaact attttctggt
      661 aaacgagtca tggtatctgg ttgatttgcc gggttatggc tatgccaagg cttcaaaaga
      721 caaacgaaag gagttttccc aaatcatcac aaattacata cttaaacgtt caagccttac
      781 cctacttttt gtgctgattg attcacgtct tgaaccccag ccaatagaca ttgattttat
      841 taactggctg ggtgtaaacc aaatcccgct agcgctggta tttactaaaa ccgataaaac
      901 gcctaaagct ctccttgagg ccaacatgga agcttttaaa aataaaatga tggagatgtg
      961 ggaggtgtta cctccaatat ttacctcctc ggccgttacc cgaactggaa gggaggatgt
     1021 gctatcctac atcgacagca taaatgcaaa ttttaagtca acccagccca aaaaggttga
     1081 acgggtctgg taaaattagt acatttgcgg gcattaacag gaaaaaattc ctaaaatgca
     1141 cagcaagcac aacattaagt tctttaccgg tcgcagctcc cggtatctct ctgaaagaat
     1201 tgccgatagt tttggcattc ctcttgggaa atccaacgtt acggtattta gcgatggcga
     1261 atttcagcct tcctttgatg aatcggtgag gggctgcacc gtttttatca tccaatcaac
     1321 ctttccacct gtcgacaacc tattcgagtt gctgctaatg gttgatgcag cccacagagc
     1381 ttcggcctac aaggttgttg cggttatgcc ctattttggt tgggccaggc aggaccgaaa
     1441 ggataaaccc cgcgtggcca ttggcgcaaa aatggttgcc aacatgctta gggcagctgg
     1501 cgttgaccgt attatgacca tggaccttca tgccgatcag attcaaggtt tcttcgatgt
     1561 accggttgac cacctttacg cctcttccgt ttttgttccc taccttaaaa gcttgcaaat
     1621 tgataatatt gccattgcct cacccgatat gggtggcgca aaacgtgcca atgcctatgc
     1681 ccgatttatg aatgcctcgc tggttctatg ctataagaac cgcgaaaagg ctaatcaggt
     1741 tgacgagatg accattattg gcgacgtacg gggtaaaaac gttgtaatac tcgacgacat
     1801 ggttgacacc gccggaacgc tgaccaaggc ttccggttta atgaaggatc agggtgcgct
     1861 atctgttagg gcatttgcca cacaccccgt tatgtcgggc aagtcatacg agctcattcg
     1921 cgattcccag attgaccagc tggtggttac cgataccata ccgctgcgta gcgacaggga
     1981 cacctccaag attaaggttc tctccgttgc cgaacttttt gccgatgtta tcgagaaggt
     2041 gtacaactac aagtctatca gcgaaaaatt tattatttag cgttgtgttt ttaaagcctt
     2101 ttcatatctt tgcagcccta aatatccctc aaaattaacg gtgtgatggg gggctgaaaa
     2161 attttcgagg gcagtcctga gtagcaccta ttggttaaca aaatttctac gatgaaaaca
     2221 attcagctta gtgccacgct gcgtagcgaa accgggaaaa aagcagccaa ggcaacacgt
     2281 aaatccgaac tcatcccggc aattatttat ggtggccaaa agaacattat gatttcgatt
     2341 ggcgaaaagg atatgaaaaa ggttgtttac acccccgatg tttttgtggt taagctaaac
     2401 gttgatggtg agaatttcga cactatcgtt aaggaaattc aatttcaccc agtatccgat
     2461 gccattctgc atgttgactt ttaccaggta agcaacgata aaaatattac cattgccctc
     2521 ccggttgtgc taatcgggca ggcagagggc accaagcagg gtggtaagtt gattcaggta
     2581 gttagaaagc taaaggtaaa tggtctagtt aagaatctgc ccgaaaatat cagcattgat
     2641 gttactcaac tagaacttgg caaaagtata aaggttggtg atttgtcgtt caaaggctat
     2701 accctggttg atcccatgag cactgtgatt gccagcgtta agtcaacccg tgctgcacgc
     2761 gaaactcaac cgactgaggc tgctcctcag cagtaaattg acatggattg aagtacctgg
     2821 ttgcagggtt tggaaacata ggaccggaat attccaacac ccgtcataac gtaggattta
     2881 tggtgttgga cgccctggcc agggcgtcca acattacttt tggaagtgcc cgttacggtg
     2941 atgttgccga gctacggcac aaaggcaaaa cttttatcct gctaaaaccg tccacctaca
     3001 tgaatcttag cggtaaggcg gtgagctact ggatgcagaa ggagaaaatt gcccttgaaa
     3061 acctcctggt ggtcgttgac gatcttgccc tgcctttagg cgttattcgc atacgctcca
     3121 gaggtagcga tggtgggcat aatgggctga aaagtaccaa cgaaacgctt ggaacacagg
     3181 actacgccag gttgcggttt ggtatcggca atggttatcc ccgcggccat caggttgact
     3241 acgttttggg tgagtggagc gatgaggagc tgaaaatcct ccccgaacga ttggctaagg
     3301 ccggagaggc aattttgtgc tttggcacaa taggcataga gcgcactatg aatctattta
     3361 acaaccagta agctaaccaa caaatgggcg ccgagagcga gcgggttgac aagtggctgt
     3421 ggatggtgag ggtttttaaa acccgctccc tggccattga ctgttgccgt aacgaaaggg
     3481 taacggtgaa cggggtgcct gttaaagcct cccacaccgt tcagcttaac gacttggtcg
     3541 cggtgcgcaa accccctgtt acgttaacat tcagggtaaa gggtttcccc ggtagccgaa
     3601 ttggtgcaaa gctggtggat gagtatgccg aaaaccttac ccctccagag gagtatcaga
     3661 agctcgatcc gtccttcagg gcttttagca tatacagaaa tcccggaaca ggccggccta
     3721 ccaagaaaga gcgtcgttcg ctcgacgact tttttgattc ggaagccgat gattcccctt
     3781 gggatgcgga cgattccaac tagattactc cactttgaag cctaagttag cgcgtggcgt
     3841 ttagttgccc gaaccggagc agtatatctt aaccatttgc tctgcaggct ggtagcccag
     3901 ctcaagtgct ttccggaaat cttcgcagcc gcccttggta tccttcacgt tcatgcgcga
     3961 aattcccctg ttgaaaaacg cttcggcatc cttaggatcg tatttaatgg cgatgtcgaa
     4021 atcgggtagg gcatccttat ggttgcccat aattgctttt gatatagccc ggttatagta
     4081 agcctgagtg aagtttttat caatagagat ggcccggttg aagtcctcga ttgacccgcg
     4141 gtaatccttc aaaaaagctt tggcaatccc acgcccgtaa acagctttca ggtagtcggg
     4201 ctttaggttg atggccttgt tgaacgactg aatagctgct ttaaaatcgc ccttgttgag
     4261 cagggctcct ccgcgtgtgc catatatcag cggatcgttg gggtctatcg aggcagccct
     4321 gtcgaggtag ccaattgcct ggtcaaaatc cttcaggttg gagtaggcct cacccaggct
     4381 gaagagcagc tccttgtcgt tgggaaggtc gttaagggca accagcaagt cggaaatcgc
     4441 tccattgaag tcctgaattt tcagcttgaa ggtgccgcgc tttttgtacg attcgggggt
     4501 ggcgttcttt ccctcaatca tcctgtcgta atcagcgatg gcgcccttaa agtcggaaat
     4561 gttggccagc gattcgcccc tgctgtagaa tgcctccagg tttgagccgt ctagctcaat
     4621 gctgcggtta tagtccttta ccgcctggtc gtactccatc atggctgcgt tgagcaggcc
     4681 gcgctggtag aaagccctgg agttggagtc gtttaatgaa atacaccggt taaggtctct
     4741 cagcgaggcg gtgtagttgc caatttttct aagggaaaca cccctgttta ggaagtagtc
     4801 aaagttcatg gagtcaatgg cgatagcttt ggtgtagtcg gcaatggcgg cagagtcgcg
     4861 gttaaggccg gcaagcgcca ttccccttcc aaaaaggtag tcaggatttt tcgggctaaa
     4921 cctgacagcc ctgttaagct gttcgtaggc ctgcccatac tgcagcgagt cgagcaggac
     4981 gattcctttt tcgtagctat tttgtgctat tctgggacgg agcagtaggt gagctgctac
     5041 cgctaccact actattccga ttacaatata cagggtagtt ttcttcttca tctcctttgt
     5101 gttgtttagt tctacacgca ctgacagcaa gcctaaccac tgcctaaccg gcccaaattt
     5161 agtttatttg actggttgga acaaccgcct gttaaatttt ttgatgtaat aagcttaaca
     5221 tgtaaccaaa ggagggtagg gtgtggtgta cactggcgtc cggaaaggga atgcaatcgg
     5281 ctggttatgt gtgcgggaac gaatgtttat gtatttattg caaaaatgta ccttagcaat
     5341 atttacggca atttattcaa attatcctct tgccattgtt ttggattgtt aatgatatat
     5401 tgcc
//
LOCUS       DAOPHX010000147         5315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_20563_length_5315_cov_5.725665, whole genome shotgun sequence.
ACCESSION   DAOPHX010000147 DAOPHX010000000
VERSION     DAOPHX010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20563_length_5315_cov_5.725665"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /gene="pyrH"
                     /locus_tag="PK502_09455"
     CDS             complement(<1..480)
                     /gene="pyrH"
                     /locus_tag="PK502_09455"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859761.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPH01083.1"
                     /translation="MIKYKRILLKLSGEALMGADAYGISTDVLNSYARQIKEVTDLGV
                     EVGIVIGGGNIFRGLKGCSMGFDRVKGDQMGMLATVINSLALQSAIEGIGGKARVLTA
                     TKMEPVGELYSKDKAIQVLSEGNACIIAGGTGNPFFTTDTASAIMQAFPSESTCMALS
                     "
     gene            complement(602..1429)
                     /gene="tsf"
                     /locus_tag="PK502_09460"
     CDS             complement(602..1429)
                     /gene="tsf"
                     /locus_tag="PK502_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPH01084.1"
                     /translation="MPNITAADVSKLRKITGAGMMDCKNALVEAEGDIDRAIELIRER
                     GKAIATKRAGREAGEGAGLAKVNANATRGAMIVLNCETDFVAKNEDFLKVAKKILDFA
                     LDTAPSNLEALKESVMEGRKVSDMITEFSGITGEKVELSYYDQVEAPMVVPYIHPGNK
                     LATIVGLSKVTDMQVAKDIAMQVAAMNPVSIDKEDVPEEVKLKELEIGREQARLEGKP
                     ENMLDKIAEGKLQKFFKESTLLNQEFIKDNKMTVRQYIQSADKDLKVLAFKRVSLNV"
     gene            complement(1453..2322)
                     /gene="rpsB"
                     /locus_tag="PK502_09465"
     CDS             complement(1453..2322)
                     /gene="rpsB"
                     /locus_tag="PK502_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPH01085.1"
                     /translation="MSRTSFQELLDAGVHFGHLKRKWNPKMAPYIFMEKNGIHIIDLH
                     KTVVKIDEAAAAVKQIAKSGRKILFVATKKQAKDIVAERIKPINMPYVTERWPGGMLT
                     NFATIRKAVKKMSSIDKMMTDGTIDTLSKREKLQVSRQRAKLEKNLGSIADLNRLPAA
                     LFVVDVQKEYIAVREAKRLNIPVIAMVDTCCDPTPIDFVIPANDDASKSIALIVDIMA
                     KAIEEGLEERKAEKEKEPAQKEKSEEQPTRTRARKVKVKVESTHNEGNAEVADSVEEV
                     AEQNTSVEENEQE"
     gene            complement(2433..2819)
                     /gene="rpsI"
                     /locus_tag="PK502_09470"
     CDS             complement(2433..2819)
                     /gene="rpsI"
                     /locus_tag="PK502_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPH01086.1"
                     /translation="MQIYNGLGRRKSAVARVYLREGKGNIVINGRDMKDYFQSELLQY
                     VVMQPLNVLNVGGNFDIKVTLDGGGVKGQAEAVRLGISRALVEYNPDNKPTLKANGFM
                     TRDSRSVERKKPGRPKARKRFQFSKR"
     gene            complement(2828..3283)
                     /gene="rplM"
                     /locus_tag="PK502_09475"
     CDS             complement(2828..3283)
                     /gene="rplM"
                     /locus_tag="PK502_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPH01087.1"
                     /translation="MDTLSYKTVSANKATVTKEWVVIDATNQVLGRLSSQVAKILRGK
                     HKPNYTPHVDCGDNVIIINAEKVKLTGHKLTQKQYVRHTGYPGGQRFSTPKEMLQRKP
                     IWVVEHAVKGMLPKNRLASDLFRNLHVYEGPEHPHEAQKPRVLDLSTFK"
     gene            complement(3392..3577)
                     /locus_tag="PK502_09480"
     CDS             complement(3392..3577)
                     /locus_tag="PK502_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01088.1"
                     /translation="MEILLLLNNRSFSRGRISMYKMFDFGYGQIEENKEIMPEKCSTL
                     HFRNPNMDKNHLRGNLT"
     gene            3744..>5315
                     /locus_tag="PK502_09485"
     CDS             3744..>5315
                     /locus_tag="PK502_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPH01089.1"
                     /translation="MHSYLDELSESQREAVTTSEGPSLVIAAAGSGKTRVLTYRIAHL
                     LNLGVPPWSILALTFTNKAAREMKERIAKIVGDSDAHKLWMGTFHSIFARILRSEAEL
                     LGYSKNFTIYDTTDSRNLIVQIIKELGLDSQVYKPGEVLSRISSAKNNLVTPQAYAAN
                     SSIIAQDVKARKSEMSRLYSLYTQRCFKSSAMDFDDLLLNINILFRDFPDVLLRYQNR
                     FRYILVDEYQDTNFAQYFIVKKLSEKYRNVCVVGDDAQSIYSFRGAKIENILNFRNDY
                     PDYKLFKLEQNYRSTQTIVNAANSVIENNQKQIKKVAFSANEVGDKIKLIKAYTDQEE
                     GFLVASEILNTIRDKSCAYSDIAILYRTNAQSRIFEETLRKRNIPYRVYGSLSFYQRK
                     EIKDLLAYFRLSVNPNDDEAFVRIVNYPARGIGSTTLQHLQELANVQNISLWETAQQL
                     KQLNHQLKPAAVKKLTDFVGMVGGFQSAVYTADAYEAAYSIARDSGILTDLKADKTQE
                     GLSRLQNIEELLNSIR"
BASE COUNT         1285 a         1300 c         1164 g         1566 t
ORIGIN      
        1 agacaaagcc atacaagtgc tttccgaagg gaatgcctgc attattgccg aagcggtgtc
       61 ggtagtaaaa aaggggtttc ctgttcctcc ggcaataatg caggcattcc cttcggaaag
      121 cacttgtatg gctttgtctt ttgagtagag ctcacctacg ggttccattt tggtagcggt
      181 gagcactctt gcttttccac caataccttc tatggccgat tgaagtgcca ggctgttaat
      241 aacggtggcc aacatgccca tctggtctcc tttcacccgg tcgaaaccca ttgaacatcc
      301 cttaagccca cgaaaaatat ttccaccacc tataactatt ccaacttcta cacccaaatc
      361 ggttacctcc ttgatctgac gcgcatacga gttaagtacg tcggtgctta tcccataggc
      421 gtcagcaccc ataagggctt caccactgag cttaagcaga attcttttat acttaatcat
      481 tttgatagta ttatcgggat gatgatgtaa aggtacaaaa aaagaatttt acccgtaagg
      541 gtcagggtaa cataaaaaaa ccccgtggta taacacgagg ttttaatgct ttgatgttgt
      601 gctaaacatt gagcgatacc cgtttaaagg ccagaacttt caaatcctta tcggcgctct
      661 ggatgtactg gcgaacggtc atcttgttat ccttgatgaa ctcctggttc agcagggtgc
      721 tttccttgaa aaatttctgt agtttaccct cggcaatctt gtcgagcatg ttttcgggtt
      781 ttccttcaag gcgtgcctgc tcgcggccaa tttcaagttc tttcagcttg acctcttcgg
      841 gaacatcctc cttgtcaatt gaaacagggt tcatagcggc aacctgcatg gctatgtcct
      901 tggcaacctg catgtcggta accttgctaa ggccgactat ggtggccagc ttattgcccg
      961 ggtgaatgta gggaactacc atgggggctt caacctgatc gtagtaggag agctccactt
     1021 tttccccggt aattccggag aattcggtaa tcatatcgga aacctttcgg ccttccataa
     1081 cagattcctt aagcgcttcg aggttcgatg gagctgtatc aagcgcaaag tctagaattt
     1141 ttttggctac cttaaggaag tcttcatttt tagccacaaa gtcggtttcg cagttgagta
     1201 caatcattgc accacgggta gcgttggcat tcacttttgc taaaccagca ccctcacctg
     1261 cctctctgcc tgctcttttg gtggcaattg cctttccccg ttccctaata agctcaatag
     1321 ccctatcaat atctccttca gcttcaacca gggcattctt gcaatccatc attcctgctc
     1381 cggttatctt tcgtagtttg gagacatctg ctgcagttat atttggcata tattttccct
     1441 ttttagaatt tactactctt gctcgttttc ttcaacgctt gtgttttgtt cggctacctc
     1501 ctcaacagaa tcggcaactt cagcattccc ctcattgtgg gttgattcaa ccttaacctt
     1561 taccttgcgg gcacgggttc gggtgggctg ttcttcgctc ttttcctttt gggctggctc
     1621 tttttccttt tcagcctttc tttcctcaag cccttcctca atagctttgg ccataatgtc
     1681 aactatcagg gcaatggatt tggaagcatc gtcgttagcc ggaattacaa agtcaatggg
     1741 agtaggatca caacaggtat caaccattgc aattaccggt atgttaagcc gcttggcttc
     1801 acgaacagcg atgtattcct tctgaacatc cactacaaaa agggctgctg gcagcctgtt
     1861 gaggtcggca attgagccga ggttcttttc cagcttggct cgctgacgcg aaacctgaag
     1921 tttttcacgt tttgataggg tatcaatggt gccgtcggtc atcatcttgt cgatggagga
     1981 cattttttta acggccttac ggatagtggc aaagttggtg agcattccgc ctggccagcg
     2041 ctccgtaaca tagggcatgt ttattggttt aattcgctcg gccacaatat ccttggcctg
     2101 cttcttggta gcaacaaaga ggattttgcg tcccgatttg gcaatttgct ttactgcagc
     2161 agcagcctca tctattttta ctacggtttt gtggagatcg atgatgtgaa tgccattctt
     2221 ctccatgaag atgtatggcg ccatcttggg attccacttg cgttttagat ggccaaagtg
     2281 cactcctgca tcgagcaact cctgaaatga tgttcttgac atggttgttt tttagtttag
     2341 attaattggc aatcgcacag tagtcccgtg ggggagtctg ggcaatttat cactaaactg
     2401 cttgccaaac tactatggga aaatttataa ttttaacgtt tgctgaattg aaatctcttt
     2461 ctagcctttg gcctaccggg tttcttgcgc tcaacgctgc gtgaatcgcg tgtcataaac
     2521 ccatttgcct taagggtggg cttgttatcg gggttgtact caaccagcgc tctggagata
     2581 cccagcctta ctgcttcggc ttggccctta acaccgccac catcaagggt aaccttgata
     2641 tcgaaatttc caccaacatt aagtacgtta agtggttgca taacgacgta ctgcagcagc
     2701 tccgattgaa agtagtcctt catatcccgg ccgttaataa cgatgttacc tttgccctcg
     2761 cgcaggtaaa ctctggctac agccgacttt cttcttccta atccgttgta aatctgcata
     2821 ccttaaacta tttaaaagtg ctcaaatcta gtactcttgg cttttgtgcc tcgtgcggat
     2881 gctcgggacc ctcatataca tgtaggttcc tgaacagatc cgatgcaagc ctgttttttg
     2941 ggagcatacc cttaacagca tgttcaacaa cccaaatggg cttgcgctga agcatttcct
     3001 tcggggtcga gaatcgttgt cctccgggat agcctgtgtg tcgtacgtac tgtttctggg
     3061 taagcttatg gccggtgagc ttaaccttct cagcattaat gataataacg ttgtcaccac
     3121 agtcaacgtg tggcgtgtag ttgggtttgt gcttgcctct aagtatcttg gcaacctggc
     3181 tcgacaacct acccagaacc tggttggtgg catcaatcac aacccactct tttgtaacgg
     3241 ttgccttgtt ggccgaaacc gtcttgtagc ttagtgtatc cactgtaaac tcctagtttt
     3301 aagttaatac ctgttgtttt gtgatccttg catttttaaa catcaagtgg gttgcaaagg
     3361 tataactatt aatttaaacc acaacacctt tttatgttaa gtttcccctt aaatggtttt
     3421 tatccatgtt tgggtttcga aaatgcaggg ttgagcattt ttcgggcata atttctttgt
     3481 tctcctcaat ttggccataa ccaaaatcga acatcttgta catggaaatt cggccccggg
     3541 agaagctgcg gttgtttagt agaagtaaaa tttccaccgt cccgatgtgt tttccttcaa
     3601 tcgtctattc ttctaaaact gttccttcgg tgctgatatt atgagactta taattatcga
     3661 agctctttca accgatgttt tgccggtcat tttttgtata gccatttttc aggactattt
     3721 ttgcaggttg aaaataccac accatgcaca gctaccttga cgagttgagc gaaagccaac
     3781 gtgaggctgt tactacttcc gagggtccca gcctggttat tgcggctgcc ggatcgggta
     3841 aaacaagagt acttacctac cgaattgccc atctgctcaa cctgggcgtg cccccctggt
     3901 cgatacttgc cctgaccttt accaataagg ccgcgcggga gatgaaggaa cgtattgcca
     3961 aaattgttgg ggattccgac gcccataagc tctggatggg aactttccac tcaatttttg
     4021 ctcgtattct ccgctccgaa gcagagctcc tgggctacag taagaatttc accatttacg
     4081 atactaccga ctcccgtaac ctgattgttc aaatcataaa agagctgggg ctcgacagcc
     4141 aggtttataa gcccggcgag gtgctgtcgc gtatatcctc agctaagaat aacctggtta
     4201 ccccgcaggc ctacgccgca aactcaagta ttattgcaca ggatgtgaaa gcccgcaagt
     4261 cggaaatgag ccgtttgtac tccctttaca cccagcgctg ttttaaatcg tctgccatgg
     4321 attttgacga tttgctgcta aacataaaca ttcttttccg cgactttccg gatgtgctgc
     4381 tgcggtatca gaacaggttc cgctacatcc tggttgacga gtaccaggat accaactttg
     4441 cccagtactt cattgtaaaa aaactctcgg agaagtacag gaatgtttgc gtggttggcg
     4501 atgatgcgca aagtatttac tccttcaggg gcgctaaaat cgaaaatatc ctcaacttcc
     4561 ggaacgatta ccccgactac aagctcttta agcttgagca aaactaccgt tctactcaaa
     4621 ccattgttaa tgctgctaac agcgttattg aaaataacca gaagcaaatc aaaaaggttg
     4681 ctttttcggc caacgaggtg ggcgataaga tcaagctgat aaaggcctat accgatcagg
     4741 aggaaggctt tctggtggcc tccgaaattc tcaacaccat cagggataag agttgtgcct
     4801 acagcgatat tgccattctg taccgaacta acgcccagtc gcgtatattt gaggaaaccc
     4861 tccgcaagcg aaacataccc tacagggttt acggtagcct atcattctac cagcggaagg
     4921 agataaagga tttgcttgcc tacttcaggc tatccgtaaa ccctaatgac gatgaggcct
     4981 ttgtacgaat tgttaactac cctgcaaggg gtattggcag caccaccctt caacacctgc
     5041 aggaacttgc aaacgtccaa aatataagcc tctgggaaac agctcaacag ctaaaacagc
     5101 ttaaccacca gttaaaacct gctgctgtaa aaaagctgac cgattttgtt ggtatggttg
     5161 gcggtttcca atcggctgtt tacacggccg atgcctatga ggcggcctac tccattgccc
     5221 gcgattccgg catccttacc gatcttaagg ccgataaaac acaggagggg ctttcccgac
     5281 tgcaaaacat tgaggagctg ctcaacagca tcagg
//
LOCUS       DAOPHX010000148         5144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_21557_length_5144_cov_6.987424, whole genome shotgun sequence.
ACCESSION   DAOPHX010000148 DAOPHX010000000
VERSION     DAOPHX010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21557_length_5144_cov_6.987424"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..787
                     /locus_tag="PK502_09490"
     CDS             347..787
                     /locus_tag="PK502_09490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM-associated putative lipoprotein"
                     /protein_id="HPH01090.1"
                     /translation="MKKAKRIILVSYNVLITCLMAILGFLSSCRDTPADEYGSPHALF
                     KAKGNVSSAAAQQPIEGIKVTSQIDTVFTGIEGQFEINAGVDLPTGQSFNLKFQDSDG
                     AANGEFQELDTIVEFNNPVFTGGDGHWYQGETEKEFNVQLKGKE"
     gene            784..1890
                     /locus_tag="PK502_09495"
     CDS             784..1890
                     /locus_tag="PK502_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04133 family radical SAM/SPASM protein"
                     /protein_id="HPH01091.1"
                     /translation="MTATAIPFRKKLALNLFKTYRRNAAKLHELNYVLWEATLRCNLS
                     CLHCGSDCKMDASVGDMPADDFLRVMDQVAEVANPNSTMVVFTGGEPLLRNDLEQVGL
                     SLYRKGFPWGIVTNGMLLSKQRIDSLVDSGLRSVTVSLDGLEASHNWLRGSGMSFSRA
                     LEAVRLLCAVPDVRFDVVTCVNRRNFGELNKLLELLLQVGVKEWRIFTIFPIGRASRI
                     PELQLQPEEFKQLFDFISWTRKENKIKLNYGCEGFLGSYEGEVRDSLFFCRAGINIAS
                     ILANGSISACPNMRDNFIQGNIYKDNFRNVWEQRFLTFRDRSWTRTGACADCDFYGYC
                     EGNGMHLRNEQNGELLFCHLQRILEGERILSGKA"
     gene            2149..3012
                     /locus_tag="PK502_09500"
     CDS             2149..3012
                     /locus_tag="PK502_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPH01092.1"
                     /translation="MKHIVVPIDFSKESLNGLRLAIIFANRFGSDIQMVHVQKFPSEY
                     TPGQQEEDRRNKEKKFMELVEEYSPKMLESANLTYILKKGKVYSEVVNQAQAFEDSAI
                     ICSTHGASGFEEFFIGSNAFRIISATDRPVITIRHGSMPRDIQKIVLPIDITTDTRQK
                     VPVTADIAKAFDAEIHVVAVASSQSDEIDLKLNAYSRQVTTYLKNREVRFQTSSLRGD
                     NITNITIEYALDVNADLISIMTEQNFSFSDFVLGSQAQQMLSKSPIPVLSVTPKEIFI
                     MGSFNVQGGGY"
     gene            complement(2987..4207)
                     /locus_tag="PK502_09505"
     CDS             complement(2987..4207)
                     /locus_tag="PK502_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01093.1"
                     /translation="MKEPNRVGWILLVAGSWGIMRFATDNETYIVPIFFSLLGGFFFL
                     RGTERSGKGSIFWSGFFSAFAILLHQVMIFWWIAIATGVIVRNRKREIFQFFCPALTV
                     PAAYLIAVWLEYGSIRVPVIVKFGLSDYYNGAAKLEFNGRVLLMWLLSLFRSFFQAHG
                     YMFNLFSQSWTWVIAGISAVMLTGYGLTNAIKNGKIQSSAFKRPVWIFALAALLHIFF
                     ALLSGGNAEFMAMLPALLAIVLSVIIDINPKALVITALGMLAWNITIGLIPLKYYTLD
                     GSSMVVNSILANPQPSKSAYLLHSEQRIKNELEYRQISQKPVLLGRARYNTQQLTDSL
                     EVLLEEGFAVYTDDYLRPQTVSREYIVEDHPASDDLWAPFRLIPCDSVVTLTGIYSLY
                     RVLPPVASSLPPER"
     gene            4427..5083
                     /gene="upp"
                     /locus_tag="PK502_09510"
     CDS             4427..5083
                     /gene="upp"
                     /locus_tag="PK502_09510"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HPH01094.1"
                     /translation="MPMVRVLGDQNSVLNQFIAELRNVNVQGDFMRFRKNMERCGEVF
                     AYELSKELSYQPEQVQTPLGVADMRLPNETLVLATILRAGLPLHQGMLNFFDTAQNAF
                     ISAYRKYNKDGSFRIQFEHLSCPNIEGKTLVLMDPMLATGASMLLAYKALLERGKPRY
                     THIVSVIASREGVEYMKRNLSEKEATIWLGAIDDEMTVKAYIVPGLGDAGDLAYGKKE
                     "
BASE COUNT         1391 a         1139 c         1297 g         1317 t
ORIGIN      
        1 aaaaaagtaa gtggtagtag ggagaaagta atgggtagta acaagataat tcagcgggat
       61 tatcagctac aaacataagg atgggcgtag tatgttgttg agagtaggga taggattgcc
      121 acacgtcacg aaccgatcca ccagccggcg gaaaacgccg aggacaaccg taaggtagct
      181 aagggatttt tattcactca ggtataaatt atttcgtgtg gtatcttgct tctccacatc
      241 acttttggtg acaatttttt tatttccttt caatatttta cccggcactt actggtttgt
      301 cctgtttttt tttaacttgc ttaaattgta gtttaatatg tcagctatga agaaagctaa
      361 gcgtataatt cttgtttcct acaacgtgct gattacctgc ctcatggcaa ttttggggtt
      421 tttatcctca tgcagggata ccccggccga tgagtatggt tctccacatg ccctgtttaa
      481 ggcaaaggga aatgtttcgt ctgctgcagc ccaacagccc attgagggta ttaaggtgac
      541 gtcgcagata gatacggtgt tcaccggaat cgaagggcag ttcgaaatta acgccggagt
      601 ggatttacct accgggcaat cgtttaacct taagttccag gatagcgacg gagcggcgaa
      661 cggagagttt caggagctgg ataccattgt tgagttcaat aatccggttt ttactggtgg
      721 cgatggccat tggtaccagg gtgagactga aaaggagttt aacgttcagc tgaaggggaa
      781 agaatgacag ctaccgctat cccttttagg aaaaagctag cccttaacct tttcaagacc
      841 taccggcgta atgcggcaaa gctgcacgag ttaaactacg ttctttggga ggcaacccta
      901 cgctgcaacc tgagttgcct tcactgcgga agcgattgta aaatggatgc ctctgtaggc
      961 gatatgcctg ctgatgattt cttgcgtgta atggaccagg ttgccgaagt tgccaatccc
     1021 aacagcacca tggttgtgtt taccggtggt gagcctcttt tacgaaatga tttggaacag
     1081 gtggggttaa gcctttaccg gaaaggtttt ccctggggaa ttgtaaccaa cgggatgttg
     1141 ctgagcaagc agcggatcga ctccttagtt gattcagggc ttaggtctgt gacggttagc
     1201 cttgacgggt tggaggcctc gcacaattgg ctgaggggct cggggatgag tttttcccgg
     1261 gcgcttgaag ctgttaggtt actctgcgct gtccccgatg ttaggtttga tgtggtaacg
     1321 tgtgttaacc ggcggaactt tggtgagctg aacaagctac tggagctgct gcttcaagtg
     1381 ggagttaagg agtggcggat attcaccatt tttccgattg gaagggcaag ccgcatcccc
     1441 gagctgcagc tgcagcctga agagttcaag cagcttttcg atttcatcag ctggactcga
     1501 aaggagaata aaattaagct caactacgga tgtgaaggct ttctgggcag ctacgagggc
     1561 gaagttcgcg atagtctctt tttttgccgc gctggcatca acattgcctc catactggcc
     1621 aatggctcta tttcagcctg tcccaacatg agggacaact tcatccaggg gaatatttac
     1681 aaggacaact ttaggaatgt gtgggagcaa cgatttttaa ctttccggga taggagctgg
     1741 acccgaacag gtgcttgtgc cgattgtgat ttttatggct actgcgaggg caacggcatg
     1801 cacctacgaa atgagcaaaa cggagagcta cttttctgcc atctgcagag aatactggag
     1861 ggagaaagga ttctttcagg aaaggcctag cttggcgggg tctagtgtga atgatctcta
     1921 ttccattaat tagggtgaag ggttcaggca agcaggtttt ttgcagctac tattcgaaac
     1981 gatttgcagc aggcggcagc aatgaggttg tgggcgattt acggaacttt gcgtaatcct
     2041 gagcctatgg ttattccaac cctgatgtaa aataaatttg acctgtcgca ttggttaacg
     2101 gaataatttg acgtagattt gcttacgttt aacctgaacc cttaagccat gaagcacatt
     2161 gtagtcccca tcgatttttc gaaggaatct cttaacggtc ttcgtttggc catcatattt
     2221 gccaaccggt ttggcagcga tattcaaatg gtgcacgtac agaagttccc ttccgaatac
     2281 acccccggac agcaggagga ggaccgtcgg aataaggaga aaaagtttat ggagctggtt
     2341 gaagagtact cacccaaaat gctcgaaagt gccaacctca cttacatcct aaaaaaggga
     2401 aaagtttaca gcgaggtggt caatcaggca caggcctttg aggattcggc tataatatgc
     2461 tcaacccatg gcgcttcggg gtttgaagag ttcttcattg ggagcaacgc tttccgtatt
     2521 atttcggcta cggacaggcc ggttataacc atcaggcatg gctccatgcc cagggacatc
     2581 caaaaaatcg tgcttccaat tgatataacc accgataccc ggcagaaagt tccggtaacg
     2641 gccgatattg ccaaggcttt tgacgccgaa attcatgttg ttgccgttgc ctcctcccag
     2701 tcggacgaaa ttgacctaaa actcaacgcc tactcccggc aggttacaac ctacctgaaa
     2761 aacagggagg tccgctttca aacatccagc ctacgaggcg ataatatcac caatattacc
     2821 attgagtatg ctcttgatgt taatgccgac cttatcagca taatgaccga gcagaacttc
     2881 agcttcagcg attttgtgct gggtagtcag gcccagcaga tgctcagcaa aagccctatt
     2941 cctgtgctaa gcgttacacc caaggaaatt ttcatcatgg gtagcttcaa cgttcagggg
     3001 ggaggctact agcaacagga ggtaaaacac ggtaaagcga gtagatccca gttagcgtaa
     3061 caactgaatc gcagggtatt aggcgaaagg gagcccacaa atcatcagat gccggatgat
     3121 cttcaacaat gtactcacgc gatactgttt gcggtcgcag gtagtcgtcg gtgtaaacag
     3181 caaaaccttc ctccagcaat acctccagac tatcggttag ctgttgggtg ttatacctgg
     3241 ctctgcccaa taaaactggt ttttgcgaaa tttgcctgta ctccagctca tttttgatcc
     3301 gctgctcgct atgtagcagg taagccgact ttgatggttg aggatttgca aggatgctgt
     3361 taactaccat actgctgcca tcaagcgtgt agtattttag aggaatgagt ccgatggtaa
     3421 tattccatgc taacattccc agagccgtaa ttaccaaggc ttttgggttg atatcgatta
     3481 ttaccgaaag cacaatggct agtagagcag gaagcatggc catgaattcg gcattaccac
     3541 cggataaaag agcaaagaaa atgtgcagta gagcagccaa tgcaaaaatc caaactggcc
     3601 gtttaaatgc tgaactttgg attttcccat tcttaatagc gttggttagg ccgtatccgg
     3661 ttagcataac ggccgaaatt cctgctatca cccatgtcca actttgacta aacaggttaa
     3721 acatgtaccc atgagcctgg aaaaacgagc gaaacaagct caacaaccac atcaagagca
     3781 ccctgccgtt aaactcaagt tttgcagctc cattgtagta gtctgatagc ccaaatttca
     3841 ctatcacagg aaccctgatg ctgccatact ccagccaaac ggcaattaag taggcggctg
     3901 gcacagttag agcagggcag aaaaattgga atatttccct ttttctattt cgaactataa
     3961 ctcccgttgc tattgcaatc caccaaaaaa tcattacctg gtggagcaga atggcaaatg
     4021 ctgaaaaaaa accagaccag aaaatactgc ctttccctga acgttcggtt ccccttagaa
     4081 agaaaaaacc tcccaacagc gagaaaaata tgggaactat ataggtctca ttatcggttg
     4141 caaaacgcat aattccccaa ctaccggcaa caagtagaat ccagcctacc ctgttgggtt
     4201 ctttcaccca gtgagatgag gggagttggc gggagtaagg taaggcaata taacagcaag
     4261 cagcgttttc ctgggcattt tggcaagtcg aatgttaatt ctgcttgatg ctgaagcctg
     4321 gctacctaag ctggcagcat ggtgaagcgg ggcgcatgat ataaaaattt gttcaagtag
     4381 atttccggtt ttaaaatctt tgtttaactt agccaaaaag aaaagtatgc cgatggtaag
     4441 ggtgcttggc gatcagaatt cggttctgaa tcagttcatt gccgagctgc gtaatgtgaa
     4501 tgtacagggt gatttcatgc gcttccgaaa gaatatggaa cggtgcggcg aggtatttgc
     4561 ctatgagctt agcaaggagc tgagctacca gccggaacag gtgcaaacac ccctaggggt
     4621 tgccgatatg cggttgccca acgaaacgtt ggttttagcc accattcttc gagccgggct
     4681 cccgctccac cagggaatgc tcaacttttt cgatacagcc caaaacgcat tcatttcggc
     4741 ctaccgcaag tacaacaagg acggctcgtt ccgtatccag tttgaacacc tttcatgtcc
     4801 aaatatagaa ggcaaaactc tagttctaat ggacccgatg ctggccaccg gagcctcgat
     4861 gctgctggcc tataaggcgc tcctcgagag gggaaaaccg cgctacaccc atatcgtatc
     4921 cgtaatagcc tcgagggagg gcgtggagta catgaaacgc aacctgtccg agaaggaggc
     4981 caccatctgg ttgggggcta ttgatgatga gatgaccgtt aaggcctaca ttgtcccagg
     5041 gttaggcgat gcgggcgatc tggcttacgg aaagaaggag tagggaggtt tttgagtccg
     5101 gatgtaagga aaaacaagac gggttcaacc gaaagatttt aaac
//
LOCUS       DAOPHX010000149         5133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_21623_length_5133_cov_4.212485, whole genome shotgun sequence.
ACCESSION   DAOPHX010000149 DAOPHX010000000
VERSION     DAOPHX010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21623_length_5133_cov_4.212485"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PK502_09515"
     CDS             <1..609
                     /locus_tag="PK502_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPH01095.1"
                     /translation="SIAAAKARDANEVIAQMANDIQLDQAAPPPPPPPPPPTEQQTVV
                     KYVAPVVVDTVKPEEQSKLMIAEEAVETVVDKEVVEVVEVVEAPKEEVEYEAPAEVFV
                     AVEEMPSFPGGDAELFKFIYENVKYPDIAMENNIQGKVIVRFCVTYKGTVDHVSVLRG
                     VDPTLDEEAIRVIKMLPQWKPGKQGGKPVNVWYTVPISFQLK"
     gene            751..1053
                     /locus_tag="PK502_09520"
     CDS             751..1053
                     /locus_tag="PK502_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01096.1"
                     /translation="MAIRITELFGAKAGDDWGRHYMGIIKHCVLPNGQQGVFGRIDIK
                     DSEHNEVACASANSREQLGVKLDLLVLMVLDYGILDLKANGMNSDTFGTNMTIGLS"
     gene            1762..4011
                     /locus_tag="PK502_09525"
     CDS             1762..4011
                     /locus_tag="PK502_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPH01097.1"
                     /translation="MRRILIVVAMILSYHILNAQNKITGKISDQANLPLIGVSVFVPD
                     MSKGTTSGTNGSYELSGLPNGKIKIQFSLLGYAGRIETVVLSGEGLELNIVLNQTALE
                     LEEIVVSGGYSSTQHENAVKIDVLKLDPRTGTTTPNFTETLTKVPGVDMISKGSGVSK
                     PVIRGLSMNDILVLNNGVRFENYQYSSHHPLGIDEFGVEDVEVIKGPASLLYGSDAIG
                     GVINFIKEKPAPIGSITGDYNMQLFSNTLGATNNFGIKGASNKFFGGLRVGQKTNSDF
                     LQGGGAFVPNSRFNEMSAKLNAGFTDKVGTFKLFYDFNNQKLGLVEDEAIEEISQRGR
                     KNEIFYQELNTHLLSSQNKLYLGKFKLDINSAFQRTELTHFGDAGVYEIQMKLATFTY
                     DAKLHLPSRVNSEYIIGLQGFNQTNTNLNNRETKLLPDATTSNYSAFGLLQYTFFRKL
                     KLQTGIRYDYKTIATKGIGVATDSMTYRAPLDKSYGSFSGSLGATYKVTEELLFRANI
                     AAAYRTPNIAELTSNGPHELRFEIGDRNLDPENSYETDVSLHYHKDNFIVDVAGFYNI
                     VNSYIFISPTGDTTASGIYIFSYKQANSILFGGEAGLHVHPKSLKWLHLETTFSSVIG
                     KQRNDLYLPFVPANKLRFELRGEKEKLALINNAFASISINTAFKQNNAAPDETPTAGY
                     TLIDLSIGGQIKVQNQLISFSISANNLTDKKYIDHLSTLKEVDLYNPGRNISLSIHVP
                     FELKASSEN"
     gene            4351..4566
                     /locus_tag="PK502_09530"
     CDS             4351..4566
                     /locus_tag="PK502_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01098.1"
                     /translation="MVRLKGGNFNEIRKGETNKLFVIVKNGMTEVAVIRINKENGCYI
                     VEKASVMRKAYVNRKELLWQRERTLLT"
     repeat_region   4691..5114
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4691..4720
                     /rpt_unit_seq="atttcaattccactatggttcggttaaaag"
BASE COUNT         1564 a         1005 c         1115 g         1449 t
ORIGIN      
        1 agcattgcag ccgctaaggc tagagacgcc aacgaggtta ttgcccaaat ggccaacgat
       61 attcagcttg atcaagctgc accaccacca cccccaccac ccccaccacc aaccgaacag
      121 caaaccgtgg taaaatacgt tgctcctgtt gtggttgaca ccgttaagcc cgaagagcaa
      181 agtaagctta tgattgccga ggaggcagtt gaaacggtag ttgacaagga agttgttgag
      241 gttgtggagg ttgtggaggc acccaaggag gaggttgagt acgaggctcc ggctgaggta
      301 ttcgtagctg ttgaggaaat gccatccttc ccgggcgggg atgccgagct attcaagttt
      361 atctacgaaa acgttaagta tcccgacatt gccatggaaa ataacatcca gggtaaggta
      421 attgtcaggt tctgcgtaac ctacaagggt actgttgacc atgtgtcggt tctccgtggc
      481 gttgacccaa ccctcgacga ggaggccatt cgggttataa agatgctacc ccagtggaaa
      541 cccggtaagc agggcggtaa gccggttaac gtttggtaca ccgttcctat cagcttccag
      601 ctgaagtagg agtaatatag ttttttgtga aaaaggcgct tcaaccgatg cgcctttttc
      661 atttacccct tgccattttt ttcgtgaata tttttgaaaa catttcaaca ggtgcatacg
      721 taaaaaatga acctaaaaaa catcgggagt atggctattc gaattaccga gctatttggg
      781 gcaaaggccg gagatgattg gggcaggcac tacatgggca tcatcaaaca ttgcgtgcta
      841 cctaatggac agcagggtgt attcggccga attgatatta aggatagcga gcataacgag
      901 gtagcttgcg ctagtgccaa tagccgtgaa cagctgggtg taaagctcga tttgcttgtt
      961 ttgatggtgc tggattatgg gatactcgat ttgaaggcga atggcatgaa tagcgatacc
     1021 tttggcacca acatgaccat tgggctaagc tgagagctgg gttgtgattt actccacgat
     1081 tttgtccttt taaaagtttt ttttgttaaa aagcggcggt tgaaatccgt gaatgttcgc
     1141 tatttttctt ttcctgtcaa caaaagcaat ttactgttgt ttacattaac gttgaagtat
     1201 tgcccaggct ctacggccag gcagttaacc ctttctattt ctttggatta agacgacagg
     1261 gtaatacatt taagcggttg ggataagggg gaaaggagtt ttttcccctc tttgttttaa
     1321 aaaatatatt tatcctactt cccatccctc ctaaaaggct gcaccagcta cttgaactta
     1381 gatgacaaaa agacctatca atattatttc aatagcattg gtgcttattt ttatagcgcc
     1441 aatgtcaatt aagcttgttg acggactttt ccatcaccat gaccattttc attgcaccgc
     1501 aacaaacgag aagcattttc atgtatatca cgaaaaatgt cccataccgg gttttgagct
     1561 atctcttttt tcggctgaaa agcatttaca aatctctcag aaacacaatt actgtgtaaa
     1621 accaaatgac gattacaact ttgtttattg tagcaataat tcagagtact catttttact
     1681 acgtgcacca ccatgtttac aggtgtgaaa tgtacatctg ttaaggtata aaagtttgtt
     1741 tataagctaa aacgtaataa aatgagaaga atacttatcg ttgttgctat gatattgtcg
     1801 tatcatatat tgaacgccca aaacaaaata acagggaaaa tttcagatca agctaattta
     1861 ccgttgattg gtgtaagcgt ttttgttccc gatatgagta agggtacaac atcgggaact
     1921 aacggatctt atgagctgtc aggtttaccg aatggtaaaa ttaaaattca attctccttg
     1981 ttgggctatg ccggtagaat tgagaccgtt gtgttatctg gagaagggtt ggagttgaac
     2041 atagtgctaa accaaacagc tttagaattg gaggaaatag ttgtttccgg tgggtatagc
     2101 tcgacccaac acgaaaacgc agtaaaaatt gatgttctca aactcgaccc acgaacaggt
     2161 acaactacgc caaattttac ggaaacactc acaaaagttc ccggcgttga tatgatttct
     2221 aaaggaagtg gtgtatcgaa accggttatc agaggactct cgatgaatga tattttagtt
     2281 ctgaataatg gggtgagatt tgagaactac cagtattcca gccatcatcc cctcgggata
     2341 gacgagtttg gagttgaaga tgttgaagta attaaaggcc ctgcttcatt gctttatggc
     2401 tccgatgcaa ttggtggtgt tatcaacttc ataaaagaaa aacccgcacc cataggtagc
     2461 attactggcg attacaacat gcaacttttt tcaaacacgc tgggtgcaac taataatttt
     2521 ggtataaaag gagcttcaaa taagtttttt ggagggttaa gagttggtca aaaaacaaac
     2581 tccgattttc ttcaaggagg aggtgctttt gtcccaaatt cacgtttcaa cgaaatgtca
     2641 gctaagttaa atgcaggatt tactgataaa gtcggcactt ttaaattgtt ttacgacttc
     2701 aacaatcaaa aactaggact ggttgaagac gaggcaattg aagaaatttc ccagaggggt
     2761 agaaaaaacg aaattttcta ccaggaatta aacacccatt tgctctcttc gcagaacaag
     2821 ctgtatttgg gtaaattcaa gctggatata aattcagcgt ttcagcgtac agagttaacc
     2881 cattttggtg atgctggcgt ttatgaaata caaatgaagc ttgcaacatt tacgtatgat
     2941 gccaagcttc atttgccatc aagggtgaac tccgaataca taatcgggtt gcaggggttt
     3001 aaccaaacaa atacaaatct caacaacagg gaaaccaagc tattacctga tgctacaaca
     3061 agtaattatt ctgcttttgg cttgttgcag tacactttct tcagaaaatt aaaactgcaa
     3121 acaggcattc gttacgatta taagaccata gccactaagg gtattggtgt ggctaccgat
     3181 tcaatgacct atcgtgcccc attagacaag tcatacggga gttttagcgg gtcgcttggt
     3241 gccacataca aagtaacgga agagctatta tttagagcaa acattgctgc tgcataccgc
     3301 actcccaaca tagcagagct tacttcaaac ggaccacacg agctgagatt cgaaattggc
     3361 gatagaaact tagaccctga aaattcatac gagacagatg taagcctaca ttaccataag
     3421 gataacttca tcgttgatgt ggcaggtttt tacaacattg tcaatagcta catatttatt
     3481 tcaccaacag gcgacacaac tgcctcgggg atatatattt ttagctacaa gcaggcaaat
     3541 tccatattgt ttggaggcga agcaggtttg catgttcatc ccaaatcgtt aaaatggctg
     3601 caccttgaaa ccacgttttc atccgtaatt ggcaagcaac ggaatgacct ctacctgcca
     3661 tttgtgccag ctaataaatt acggtttgaa ttacggggtg aaaaggaaaa attagcgctt
     3721 attaataatg catttgcctc tataagcata aacaccgcat ttaagcaaaa caacgctgcg
     3781 cctgacgaaa cgcctactgc tggatatacg cttattgatt taagcattgg tggccaaatt
     3841 aaagtgcaga atcagcttat ttcatttagc ataagcgcaa acaacctaac ggacaagaaa
     3901 tacattgacc atctttcaac actgaaagaa gtggatttat acaatcccgg tcggaatatt
     3961 tcgttgagca ttcatgttcc attcgagtta aaagcaagta gtgagaatta acagctacta
     4021 aaaaggggct atgactttcc ccatttttag gaacgcttat agccatttcc tggctgaatt
     4081 ttagctttaa ggcctgatgc tgatggggtg ttaataaccc gaaatagctc gcagggctac
     4141 tcgggctgtt ggcacccctt cctgctcctg gtagctccat tcttcctttt tcccattttt
     4201 ttatgccggc ttaccgtata gcacggctgt ggttttcctg tggggcaccg accctaatgg
     4261 cgggcgtaaa tgtattcctt gttgatatcg tggcaccacc gggtttagct tttttcacca
     4321 taactagtgg tgcctttttt caattccact atggttcggt taaaaggggg taactttaac
     4381 gaaataagga aaggggagac aaataagttg tttgtaatag ttaagaatgg gatgacagag
     4441 gttgccgtta ttcggataaa caaggaaaac ggatgctaca ttgttgaaaa ggcaagtgtg
     4501 atgcggaaag catatgtaaa cagaaaagag ttgctgtggc aacgggagcg aaccctatta
     4561 acctgaactg ccctcaggtt tctttgacct gctatgctgc ttatttgggc aggcagcgat
     4621 aacagaacgc ttagaagcac acaacaactc ctaaatacaa aggtaaacca ttttaaggtt
     4681 aaagtcaaat atttcaattc cactatggtt cggttaaaag cataaatagg aagaaaacca
     4741 ttaatctcct ggtacatttc aattccacta tggttcggtt aaaagaagta aaccgctata
     4801 atatagaaca caataaacga aatttcaatt ccactatggt tcggttaaaa ggaaaaatag
     4861 cttaacaact taccgttacg tgaacgcatt tcaattccac tatggttcgg ttaaaaggaa
     4921 gatatccgag gatgataaaa ttgtaataga caaatttcaa ttccactatg gttcggttaa
     4981 aagtgtttta tacgttgtgg cagaagttca aggaggagat ttcaattcca ctatggttcg
     5041 gttaaaagaa agcgagagcc cggctaaaaa caagccgagt aaaaatttca attccactat
     5101 ggttcggtta aaagatgttg aaggttatca ttt
//
LOCUS       DAOPHX010000150         5110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_21750_length_5110_cov_7.151533, whole genome shotgun sequence.
ACCESSION   DAOPHX010000150 DAOPHX010000000
VERSION     DAOPHX010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21750_length_5110_cov_7.151533"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PK502_09535"
     CDS             <1..457
                     /locus_tag="PK502_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPH01099.1"
                     /translation="NRGFYLDPHVVRAVGDSIVRNSNVPEKHYTTIDTQWFDYIVEGM
                     DMAVNGAPGSGSTARIAALPDIRICGKTGTSQNPHGADHSVFFAFAPKDDPKIAISVY
                     VENAGFGATWAAPIASLMIEKYLRDSISRPYLEDYILQANLLDRRAKKK"
     gene            441..1868
                     /gene="rodA"
                     /locus_tag="PK502_09540"
     CDS             441..1868
                     /gene="rodA"
                     /locus_tag="PK502_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPH01100.1"
                     /translation="MQRKSNLVSNLDWGTIVLYVILVLMGWMNIYAAVYSEEHNSILD
                     FSQRYGKQLIWITFAFFLGVVVLLSNGRIYSVFAYIIYGLVILLLLSTLVFGRVVNAS
                     KSWIPLGSFGIQPAEFAKFATALALARLMSPYEFKLNTFWSYVKVGLIIAIPAGIILL
                     QNDTGTALVFGSFVLVLYREGLPWWVLGVLFLLVALFISTLVFSTPVIIVALYVTTVL
                     IYMLQRGKFKFAISLTAGLTGLGFLLYLASQTFNIQVKTDYFYIIPVGMGIVIATLYA
                     FFSRTKMIYGLVLFLCVTVGFSLSTEYVFENVLDVHHQKRINDLLGIESDPLGWGYNV
                     NQSKIAIGSGGFWGKGYLSGTQTKFNFVPEQSTDFIFCTVGEEWGFVGSLVVLALFAW
                     LLLRLIRIAERQRESFSRIYGYGVVSILFFHIAVNIGMAVGLLPVIGIPLPFFSYGGS
                     SLWAFTILVFILLRLDADRYGTFKG"
     gene            complement(1858..2445)
                     /locus_tag="PK502_09545"
     CDS             complement(1858..2445)
                     /locus_tag="PK502_09545"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HPH01101.1"
                     /translation="MNFLAHLYLSGCNPEVRLGGFIGDYVKGSKFTHFTPDIQKGIKL
                     HRAIDSVTDSHPSTRACSAIFKPVYKRYSGVVSDVVFDHVLAKEWSRYSESNLNEFTQ
                     IFYTQMFQRYQLLPPTVQQFLPNMIRTNRLSSYQTSEGVREALNIMSLRTSLPNNTEY
                     AIVQLKTHYNLFFDYFHPLFNDLMQLATREFGINL"
     gene            complement(2506..3351)
                     /locus_tag="PK502_09550"
     CDS             complement(2506..3351)
                     /locus_tag="PK502_09550"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPH01102.1"
                     /translation="MNQNRKIYFASDLHLGHPNLRDSLPREKLFISWLDSIDADIEEL
                     YLLGDIFDFWFEYRRVVPKGYTRFMGRIAQLTDRGIPVHFFTGNHDVWMFNYFKEELG
                     VEIHHKPIEKVLYGHKVLMAHGDGLGPGDIGYKLLKRIFTSTTLQWLFARLHPNFSLW
                     FGNQWSVSSRYSKEITHVFRGDDEQITAYARQVLDSKHFDIFMFGHWHSPTIFPLTQD
                     SNLVVLGDWLVSNTYAVLDGDDFWLMKYFPDKPDVPLGHIKLSHVPEAPVLPLHDQFN
                     RINKY"
     gene            complement(3417..4583)
                     /locus_tag="PK502_09555"
     CDS             complement(3417..4583)
                     /locus_tag="PK502_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPH01103.1"
                     /translation="MDCKHTRFINAFLLALPLFILSCSNGSKVTIRVEVKSPEKNMLY
                     ISRIDFGKTTPIDSAKISSGESIKKFRLPQGSEPTFYTISLKKGGALTLLASRDEKIS
                     VKFDVQNMDSYEVVGSKESLKVKEIAESFRKSKQTVDRLRKEYIKAQTDAEREAIGRQ
                     FQAEIDSQRASCSRFIWANPMSKASIMALYQKYNDDLYLFDRAEDLTLLKMVASAWRA
                     LYPESDYTRGMLEDIGRIEKIITNSKINKLISEAEPSIPDLDIPDKNGTFVKLSSLKG
                     KVILLDFFTASNTSSLLDNRELMNIYNTFKNRGFEVYQVSMDADKNEWLNYLSNTRVT
                     WISVREVDPTHSAAALLYNVTQLPTNYLIDKNFNIIDKNIYGERLKEKLNVLLR"
BASE COUNT         1292 a         1085 c         1223 g         1510 t
ORIGIN      
        1 caacaggggg ttttaccttg atccccatgt ggtgagagct gtaggtgaca gcatagtgag
       61 aaactcaaat gttcccgaaa agcactacac cacaatagac acccagtggt tcgactatat
      121 agttgaggga atggatatgg ccgttaacgg agcccccggg agcggaagta ccgcccgaat
      181 tgctgctctc cccgatattc gcatttgcgg gaaaacggga acatcacaaa atccacatgg
      241 agccgaccac tccgtatttt ttgcctttgc tcccaaagat gatcctaaaa ttgccatttc
      301 ggtgtacgtg gaaaatgccg ggtttggtgc aacttgggct gcgccaattg ctagcctaat
      361 gattgaaaag tacctacggg actctatatc ccgtccctat ctcgaagact atatacttca
      421 agcaaaccta ctcgaccgcc gtgcaaagaa aaagtaacct ggtaagcaac ctcgattggg
      481 gaaccatcgt gctttacgtc atccttgtgt tgatggggtg gatgaacatc tatgccgcag
      541 tttacagcga ggagcacaac agcatactcg attttagcca gcgctatggc aagcagttga
      601 tttggattac ttttgccttt ttcctgggag tcgttgtttt gttaagcaac ggaaggatat
      661 attccgtttt tgcctacatc atctacgggc tggttattct tttgctcctt tccaccctgg
      721 tgtttggcag ggtggttaac gcctcaaaat catggattcc actcggatca tttggaatac
      781 agcctgcgga gtttgccaag tttgccactg cacttgctct cgccaggcta atgagcccat
      841 acgagttcaa gctaaataca ttttggtcat acgttaaagt tggtttaatc attgctattc
      901 ctgccggtat tattctgcta cagaatgata ccggaacggc attggttttc ggttcttttg
      961 tgcttgtgct ctaccgcgag ggattgccat ggtgggtgtt gggagtgctt tttttgctgg
     1021 ttgcgctttt tatttcaacc ctggtattca gcacgccggt tattatagtt gcgctgtatg
     1081 ttactaccgt tttaatatac atgctgcaac gggggaagtt taaatttgca ataagcctaa
     1141 cagcaggcct taccggtttg ggctttctac tttaccttgc ctcacaaaca ttcaacatac
     1201 aagtaaaaac cgattatttt tatattatcc ctgtaggcat gggtattgtc atagcaacgt
     1261 tatatgcttt cttttcgagg acgaaaatga tttacgggtt agtgcttttc ctatgcgtta
     1321 cagtaggttt ttcgctttcc accgagtatg tttttgagaa tgttctcgac gttcaccatc
     1381 agaagcgaat taatgatttg cttggcattg aatccgatcc gctgggatgg ggctacaatg
     1441 ttaaccaatc caaaattgcc attggttcgg gtgggttttg gggaaaggga tacctttcgg
     1501 gaacccaaac aaagttcaac tttgttcctg agcaaagtac cgattttatt ttctgcacgg
     1561 tgggtgagga gtggggtttt gtaggttctt tggtagttct tgcgcttttc gcatggctgc
     1621 tcttgcgtct tatcaggatt gccgaacgac aacgtgagag tttttctagg atatacgggt
     1681 acggggtagt gtccatccta ttttttcata ttgcagtaaa cattgggatg gcagttgggc
     1741 tacttcccgt tataggaatc cctttacctt tttttagcta cggaggctca tcgttgtggg
     1801 cttttactat cctggttttt atcctgctaa ggcttgatgc cgacaggtat ggaacattta
     1861 aaggttgatg ccgaactccc tggtcgcaag ctgcattaaa tcgttgaaaa gcgggtgaaa
     1921 ataatcgaaa aataggttgt agtgagtttt cagctgcaca atggcgtact ccgtgttgtt
     1981 gggaagtgag gttcggaggc tcataatgtt gagtgcttcc cgaacccctt cacttgtctg
     2041 gtagctcgaa agccggttgg tgcgtatcat gttggggagg aattgctgaa cggtaggggg
     2101 gagaagctgg tagcgctgaa acatttgggt gtagaagatt tgtgtgaatt cattcaggtt
     2161 actttcagag tagcggctcc actcttttgc caaaacatgg tcaaaaacta catcactcac
     2221 cacacccgag tagcgcttat acactggttt aaaaattgcc gaacaggctc gggtggaagg
     2281 gtggctatcg gtaacactgt ctattgccct atgaagttta ataccctttt gaatatcggg
     2341 agtgaagtgg gtaaatttac taccttttac atagtcgcca atgaagccgc ccaatctgac
     2401 ttcaggatta caacccgaaa ggtagagatg ggctagaaaa ttcacagggt tgatgtttgc
     2461 ttttggttaa ttgcactggt ggaagcgctg aataagccga tctacctaat atttattaat
     2521 tctgttaaat tgatcatgaa gcggcaaaac tggtgcttcg ggaacatggc tgagcttgat
     2581 atgccctaag ggcacatcgg gtttatcggg gaaatacttc ataagccaaa aatcgtcacc
     2641 atcgagcacg gcataagtgt tgctaaccag ccagtcgcct agcacaacca ggttggaatc
     2701 ctgcgtaagg ggaaaaatag ttggtgaatg ccagtgacca aacatgaaaa tgtcaaaatg
     2761 cttgctatcc aaaacctgcc gggcgtaagc ggttatttgc tcgtcgtcgc ctctgaaaac
     2821 atgggttatc tcttttgagt agcggctgct aacgctccac tggttcccaa accaaagtga
     2881 aaagtttggg tgtagcctgg caaaaagcca ctgaagggtg gtgctggtaa aaatgcgttt
     2941 caacagcttg tagccaatat ctcccggtcc aaggccgtca ccatgcgcca tcagcacctt
     3001 atgcccgtaa agcaccttct ctatgggttt atggtgaatc tcgacaccta gctcctcttt
     3061 aaaatagttg aacatccaaa catcgtggtt tccggtgaag aaatgtaccg gtatccccct
     3121 atcggtgagc tgagctatac gtcccatgaa acgggtatac ccctttggaa ccaccctgcg
     3181 gtactcaaac cagaagtcaa aaatgtcacc cagcaggtag agctcctcaa tatcagcatc
     3241 aatactatcg agccacgata tgaaaagctt ctcacggggg aggctatctc taagatttgg
     3301 atgcccaagg tgaaggtcgg aggcaaagta tatttttcga ttctgattca ccggcttaaa
     3361 taagtgtttt tgaacatctc tacatgctga atggttactt ggcgtttagg cttagcctac
     3421 cttagaagga cattcagctt ctccttaagt ctttcaccgt atatgttttt atcaataatg
     3481 ttgaagttct tgtcaatcag gtagtttgtt ggcagctgtg taacgttgta aagtagggca
     3541 gcagctgaat gtgtaggatc aacttccctg accgaaatcc atgttactcg ggtatttgac
     3601 aggtagttaa gccattcgtt tttatcggca tccatggaaa cctggtaaac ctcaaaaccc
     3661 ctatttttaa aggtgttata aatattcatc aattcacggt tgtcgagtag gcttgaggtg
     3721 ttggatgcgg tgaaaaagtc aagaagaatt acttttcctt taagtgatga tagctttaca
     3781 aaggtaccat ttttatcagg gatgtccagg tcgggaatag atggttctgc ttccgaaatc
     3841 agcttgttta ttttagagtt ggtaataatt ttctcaatcc tgccaatatc ctcaagcata
     3901 cccctggtat agtccgattc ggggtagagt gcccgccatg ctgaggctac cattttgagc
     3961 agggttaaat cctcagcccg gtcaaacaag tagagatcat cgttatattt ctggtaaaga
     4021 gccataatgc tggctttcga catagggtta gcccagatga acctgctgca gctggctctt
     4081 tgcgagtcta tttctgcctg aaactgcctt ccaatagcct cacgttcggc gtcagtttgg
     4141 gctttgatgt actcctttct aagcctgtca acggtttgct ttgattttct aaatgattcg
     4201 gcaatttcct taactttcaa cgattccttt gagcctacca cctcgtagct gtccatgttt
     4261 tggacatcga acttaaccga aatcttttca tccctgcttg cgagtagagt aagtgctccc
     4321 ccttttttca ggctgatggt gtagaatgtg ggttcgctgc cctggggtaa cctaaatttt
     4381 ttaatgcttt ctccggatga tattttagct gagtctattg gggttgtttt accaaagtca
     4441 atccggctta tgtaaagcat gtttttttct ggtgacttca cctcaaccct tatggtaacc
     4501 ttgctgccat tggagcatga aagaataaac agcggtaggg ccagaagaaa ggcatttatg
     4561 aatctcgtat gcttgcaatc catttttgtt ttctatttac cgaatgcaaa agtaaccatt
     4621 atgttcaact ttcaaatagc gtggaggggg aaatcgaggg taaatgataa aggcgaaagg
     4681 cgaaaggata aaggataaag tgtaaaggat aaaaaaattg tctgggtaac gacgaaactt
     4741 gtccgcctac cggcggattc caaattccag atttcagatt ccagaacttg tccgactacc
     4801 ggcggagctt gtccggctcc tgacggattc acccttcgac aaactcaggg caggctcctt
     4861 cgacaggtgt tacagtgagt agttcgtatg gtgttatagt gagccagtcg aactactcac
     4921 tataacattt gtcgaactgc tcagggttca gacttgtcca ccttacagtg gcttacggat
     4981 taaagaactt gtccgatgtt ttaagccccg aaggggcgaa ataagtagca aggggcgcag
     5041 ccccttgtat ggagggaacg atttttacaa gccctgaagg ggcggaatag ttttgaaacg
     5101 atgagcctgg
//
LOCUS       DAOPHX010000151         5069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_21985_length_5069_cov_5.457320, whole genome shotgun sequence.
ACCESSION   DAOPHX010000151 DAOPHX010000000
VERSION     DAOPHX010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21985_length_5069_cov_5.457320"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PK502_09560"
     CDS             complement(<1..791)
                     /locus_tag="PK502_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01104.1"
                     /translation="MTILKKLLPVGICLCLTIAGKPQSKLEIEEIYRDANSYFYFEDY
                     EEALTLYLQVYSHQPENYNLCYRIGFCYLNIPGSKKNAIPYLEKAAGNITRFYREESI
                     LETKAPPDAIFYLGNAYLVNNQVENAIKEYEKFWTLTRGKGSWNFDYLNHQMATAKSS
                     IQLQKNKVNFILTNLGEPVNDHFPNFNATISGNGTTLAYTSRLKFYNAIMVAQLNERG
                     EWEKPINITLDLQVDGRCETLSLSYRGDELYLFRDDNHDGNIYVS"
     gene            complement(926..2227)
                     /locus_tag="PK502_09565"
     CDS             complement(926..2227)
                     /locus_tag="PK502_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01105.1"
                     /translation="MRLRILLIFIAAFPFQNAFCQKSSYFKRVFVDAEYYLLYEEYRD
                     ALPLYQEIYKAYPSNCNLAYRIGLCYLNIPNEKKKSLPYFEKAITSITPSYQEGYFTE
                     TKAPREAFLRYGQALRIDSQFDKAMEAFKSYQNLLTPKDLLEQKIIAREIESIAYARK
                     QLANPASHTIKPVNRNVNTRFPEVNPLSDTTGNLLIYTSLQQFYNAILVSQRKEDLWT
                     NPINLNAQLFADGAIKTVGISPNGNMLILARNDNDTYNLYFSRYDRTKNTWSPIEKFP
                     KEINSSGWEISGSLSSRGDTLYFSSNRAGGFGGFDIYMSVKTITGWSNPVNLGNKVNT
                     PFDEISPFVTENGKQLFFSSNGHRSMGGFDLYLSGYENKTWGLPHNLGYPINTLDDDT
                     FLFPLNNGKRGFISRASEESSGEDDIYEVTLDLSTLLNGDK"
     gene            2431..2886
                     /locus_tag="PK502_09570"
     CDS             2431..2886
                     /locus_tag="PK502_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPH01106.1"
                     /translation="MSLEERISQDIKTAMLAQDKVRLDALRAIKSAILLAKTEGEHKD
                     TLAPESEARLLQKQYKQRKESAEQYVQAGRQELADKELAEAAIIEEYMPKMLSADELK
                     AELVKIIGQVGAKAPSDLGKVMGVASKLLAGRADGKAISDMAKQLLGGS"
     gene            complement(2883..3212)
                     /locus_tag="PK502_09575"
     CDS             complement(2883..3212)
                     /locus_tag="PK502_09575"
                     /inference="COORDINATES: protein motif:HMM:NF021468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HPH01107.1"
                     /translation="MANYRVKPEDIKELIPQKSYCYASEMITVDGMPVGYMYREKPED
                     DDDSGWRFYSGAETEEYVEDVYHFMMFDLNYIANCDASIIPYLNSRVGTELERVEGTD
                     KFQPVED"
     gene            complement(3251..4870)
                     /locus_tag="PK502_09580"
     CDS             complement(3251..4870)
                     /locus_tag="PK502_09580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPH01108.1"
                     /translation="MAHRYRYELLLAIVTGILLLAGLGKIEVNIMEARNFTTAREMVQ
                     NREYLLTTLNGEPRYQKPPLPTWLTTLSGMAFGFDSLFTLRLPVVAITFLLVFAFYFL
                     SIALGLSKKQSFNNALILITSFYIFFGGRDNQWDMYTHAFMVTSIFFLWRLLQKDTHR
                     LGNSIAAGVFFGLSILSKGPISLYALFLPFLISYGMVYRFHLKGKGIYFINMLFIGLL
                     VGLWWYAYVRLKDPEYFRAIVGHEAANWASYETKPFYYYWNFFIQSGLWTLPSLTALA
                     YPYLKKRVDNLKAYRFSLLWTLFSVILLSAVPEKKPRYLLPTLIPMAINTGFYIDYLF
                     RRFSKGLPRAEKLWVYFSFGLLGFIALAYPFALTFILKTDILNYLHIAIISSILMLTC
                     AFFILRGMKSFNFQITFYSTIALFTVVVVAIIPLSQNLFTNPAYKPASSALNVEAKYG
                     VKTYRLNDIAPEIVWDFGKVIPSLPKINENIQAPPEKQFGLLVARSDSTIVRSGFPNF
                     EVKKVHTIDMNYRPRHRDRLIRDYYVLRLKE"
     gene            complement(4860..>5069)
                     /locus_tag="PK502_09585"
     CDS             complement(4860..>5069)
                     /locus_tag="PK502_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HPH01109.1"
                     /translation="TLRFVYQWIYSERRKESSLPMGFWVLSLAGSTLIFIYAIFRLDP
                     VLLLGHSTGIIMYSRNIYLLKKYGT"
BASE COUNT         1362 a         1045 c         1265 g         1397 t
ORIGIN      
        1 tgggaaacat aaatgtttcc atcatgattg tcgtccctga agaggtagag ctcatcgccc
       61 ctgtacgata gcgaaagggt ttcgcatcgg ccgtcaacct gcaggtcgag ggtgatgttg
      121 atgggttttt cccactcacc ccgctcgttc agctgcgcca ccatgatggc gttgtagaat
      181 tttagccttg atgtgtatgc aagggtggtt ccattgcccg aaatggtggc gttgaagttg
      241 ggaaagtggt catttaccgg ttctcccaaa ttggtcagaa tgaagttaac cttatttttc
      301 tgcagctgta tggagctctt tgctgtggcc atctgatgat ttagatagtc aaaattccag
      361 ctacccttac cccttgtcag cgtccagaat ttttcatact ccttaatagc attttccacc
      421 tggttattta ccaggtaggc atttccaagg taaaaaattg catcgggtgg ggcctttgtc
      481 tcaagaatag actcctccct gtaaaaacgg gtaatattcc cagctgcctt ttccagatag
      541 ggaatggcat tctttttact ccccggaatg ttcaggtagc agaagccaat ccggtagcaa
      601 aggttgtagt tttcgggttg gtggctatat acctgaaggt ataatgtcag ggcctcttcg
      661 tagtcctcga agtaaaagta ggagttggcg tcacgatata tttcctcaat ttctaatttg
      721 gattgtggtt ttccggctat ggtgaggcat agacaaattc caacaggtag gagtttcttt
      781 aagattgtca ttgatgcact tgttttcctt caaaggttca tttctttacc ggtttgtccc
      841 tgcggaatga ggctctaagc tcaaaacatt tttcatatga gttggacacg ttatagttgc
      901 tttatgccgc tattgtaaca atccgtcatt tatccccgtt aagtaaggtg ctgaggtcaa
      961 gggttacctc gtagatgtcg tcctcgccgc ttgactcctc cgaggctcga gaaataaatc
     1021 ctcttttacc gttgttgagt gggaatagaa atgtatcgtc gtcaagggtg ttgatagggt
     1081 agcccaggtt atggggtaac ccccatgttt tattctcata accggagagg tacaggtcga
     1141 atcctcccat ggagcggtgt ccgttggagc tgaagaagag ctgcttgcca ttttcggtaa
     1201 caaatgggga tatttcatcg aaaggagtat tgactttatt tcccaggtta accgggttag
     1261 accaacctgt tatggttttc accgacatgt agatgtcgaa tccgccaaaa ccacctgccc
     1321 tgttactgct gaagtatagg gtatcgccac gactggagag cgagcccgaa atttcccatc
     1381 cgctggagtt aatctccttt gggaacttct caatgggcga ccatgtgttt tttgttctgt
     1441 cgtaccggct gaagtagagg ttataggtgt cgttatcgtt acgcgccaga ataagcatgt
     1501 taccgttggg tgaaatgccc acagttttaa tagccccatc ggcaaatagc tgggcgttaa
     1561 gatttatggg gttggtccag aggtcttcct tgcgctggct aaccagaatg gcgttgtaga
     1621 actgctgaag agaggtgtag ataagcaggt tgcccgttgt atcggaaagg gggttaacct
     1681 cggggaaacg ggtattcaca ttccggttaa cgggtttaat ggtatggcta gcggggttgg
     1741 cgagctgctt cctggcgtag gcgatggact caatttccct ggcaattatt ttttgctcca
     1801 agagatcctt tggagtaaga aggttttggt acgatttaaa agcctccatg gccttatcga
     1861 actgcgagtc aattcgtaag gcttgtccgt aacgtaggaa agcttcacgc ggtgctttgg
     1921 tttcggtgaa atagccttcc tggtatgatg gggtgatgga ggtaatagcc ttctcgaaat
     1981 agggtaggga tttctttttt tcgttgggaa tgttgaggta gcaaagccct atgcggtagg
     2041 ccaggttaca gtttgaaggg taggccttgt atatttcctg gtaaagaggt agcgcgtcac
     2101 gatactcctc gtaaagcagg tagtactccg catcaacaaa aactctttta aagtaggagc
     2161 ttttctgaca aaacgcattt tggaatggaa aggcagctat aaatatcaac aaaatcctca
     2221 gtctcatcaa tcggcagttt acaaccctaa aggatttatc caaccaaatt gtttagaaat
     2281 aacacctcaa ttttaaaaca accagcaaga tatgacaaat agctaaacaa ctttcaaaag
     2341 tagtaaatta ttttaattca aataaatagt taaagtcaaa ttattccaac tttttctaac
     2401 tttgtgtcag aaaccataat ttgcgaggag atgtcgttag aggaaagaat cagccaggat
     2461 atcaaaacag ccatgctggc acaggacaag gttaggctgg atgcgctacg ggcaataaaa
     2521 agtgccattt tgcttgcaaa aacagaaggc gagcataagg atacgcttgc tcctgaatcc
     2581 gaggctaggt tgctgcaaaa gcaatacaag cagaggaagg agtcggctga gcagtacgtt
     2641 caggctggcc ggcaggagct ggccgacaag gagctagccg aagctgccat catagaggag
     2701 tacatgccca aaatgcttag cgctgatgag ctgaaggctg agctggtaaa aataattggg
     2761 caggttggcg ctaaggcacc ttcggacctg ggaaaagtta tgggtgttgc cagcaagctg
     2821 ctagccggca gggctgatgg aaaggctatt tccgacatgg ctaaacagct tctgggtggt
     2881 agctaatcct ctaccggttg aaacttatcg gttccctcaa cccgctccag ctcagtgcca
     2941 acccttgagt taaggtaggg aataatggag gcatcgcagt tggcaatgta gttgaggtca
     3001 aacatcatga agtggtaaac gtcttcaacg tactcctcgg tttcggcacc cgagtagaaa
     3061 cgccagccgc tatcgtcatc atcctcgggt ttctccctat acatgtatcc aacaggcatt
     3121 ccatcaactg taatcatttc cgaggcgtag cagtaagact tttgaggaat tagttccttt
     3181 atgtcctcgg gtttaaccct gtagtttgcc atagctttag gtttaacttt tgcaatttat
     3241 ttgattaccg ttattctttc aacctcaata cgtagtagtc tctaataagc ctgtccctat
     3301 gtcggggtct atagttcatg tcaatggtgt gaactttttt tacttcaaaa ttcgggaagc
     3361 ctgacctaac tatggttgaa tcgcttcggg caacaagcag cccaaactgc ttttctggcg
     3421 gagcttgtat gttttcgtta attttgggta gggaaggaat gaccttgcca aaatcccata
     3481 caatttcggg ggcaatgtcg tttagccggt aggtttttac gccatacttg gcttctacat
     3541 tcaatgcact gcttgcaggc ttataggctg gattggtaaa caaattttgc gataatggaa
     3601 ttatagccac cacaaccact gtaaaaaggg caatagtgga gtagaatgtg atttggaagt
     3661 taaaactttt catccccctg agaatgaaaa atgcgcatgt tagcatgagt attgacgaga
     3721 tgatggcaat atgtaggtag tttagtatat cagttttaag gatgaatgtt aaggcaaaag
     3781 ggtatgccag agcgatgaat cctaatagtc caaatgaaaa gtaaacccac agcttctcgg
     3841 ctcttggcaa acctttgctg aatcttcgaa aaagataatc tatgtaaaag cctgtgttga
     3901 tagccattgg aattagggtg ggtagcaggt aacggggttt cttctcggga acggctgaga
     3961 gtagaataac tgaaaacagc gtccaaagca gcgagaatct gtaggccttg aggttgtcca
     4021 cccttttttt gaggtatgga taggctagtg cggttaatga gggtagtgtc cacaacccgc
     4081 tctgtatgaa gaaattccaa taatagtaga agggttttgt ttcgtagctg gcccagtttg
     4141 cagcctcatg tccaactata gcccggaagt actccggatc cttcaaccga acgtatgcgt
     4201 accaccataa gccaaccagc aatccgatga aaagcatgtt tataaagtag atgcctttcc
     4261 ccttaagatg gaaacggtaa accatgccat agctgattag aaagggcagg aaaagggcat
     4321 agagtgagat ggggcctttg cttagtattg acaatccaaa aaatactccg gcggcaatgc
     4381 tattgcccag gcgatgggta tccttttgta gtagcctcca cagaaagaat atggaggtta
     4441 ccataaaggc atgggtgtac atatcccact ggttatccct tccaccaaag aatatgtaga
     4501 aagaggttat caggataagg gcgttgttga agctttgttt ttttgacaaa cccaacgcta
     4561 tggaaaggaa gtagaaggca aaaaccagta ggaatgttat ggcaacaacc ggaagtcgaa
     4621 gggtaaaaag tgagtcgaaa ccgaatgcca ttcccgaaag ggtggtcaac caggtgggca
     4681 aaggcggctt ctggtagcgg ggctcaccgt tgagggtggt caacaggtac tccctgttct
     4741 gaaccatctc gcgggcagta gtgaagttcc ttgcctccat aatgttgacc tcaatcttac
     4801 caaggcctgc cagaagcaga atcccggtta caatggccag cagcaactcg taacgatacc
     4861 tatgtgccat acttttttaa caggtagata tttcgggagt acatgataat gccggttgaa
     4921 tgcccgagca gcagcacagg gtcaagccta aaaattgcgt aaatgaatat tagggtagaa
     4981 ccggctaggc ttagcaccca aaatcccatg ggcagcgacg attcttttct ccgctcggag
     5041 tatatccatt ggtaaacaaa cctcagggt
//
LOCUS       DAOPHX010000152         5057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_22067_length_5057_cov_6.163135, whole genome shotgun sequence.
ACCESSION   DAOPHX010000152 DAOPHX010000000
VERSION     DAOPHX010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22067_length_5057_cov_6.163135"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PK502_09590"
     CDS             <1..372
                     /locus_tag="PK502_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01110.1"
                     /translation="WQTIPSVVGKNWLFGLGIGDVRETLAKAYQEKNMDFALNKRLNA
                     HNQYLETYVGLGLIGLSCLLLILGSALWQSIRRRDMVFFLFMLIILINFMFESVLERF
                     FGVLFFVFFLSIFSMREVDSV"
     gene            complement(424..1419)
                     /locus_tag="PK502_09595"
     CDS             complement(424..1419)
                     /locus_tag="PK502_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01111.1"
                     /translation="MSDEQVNKTKATDEVDLIELFNRMGRGIKRGTVWLFKQVENLFI
                     LLIRKSLWIVSFAIIGLLVGYLIYKSSPRYYSSEMTAISNSVDNSHIVNSINLLNSLF
                     KEKNYSSASAYLDIPVGKAKQIKSIAAFYGIDLNRDKIVDYVDYANSFRFNPKDTMAK
                     RLTDYFFVSLEVYNEGAFTAAREGLKKYIYKNEFVVENNNERIAQNEEMIRVINVEIK
                     KIDSLQKIAYFETPLLQKASNGQMVFLNEKEVKLYHDQLLKLQRDKMRLERENKINKD
                     PITIVQDFTPLSKAENPYSKYGVKWGLIFAVIGFIASLLWQFRTKIVDYIKVKHY"
     gene            1502..2524
                     /gene="galE"
                     /locus_tag="PK502_09600"
     CDS             1502..2524
                     /gene="galE"
                     /locus_tag="PK502_09600"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPH01112.1"
                     /translation="MDKILVTGGAGYIGSHTVVELVGAGYEVVLVDNFSNSSPEVLQG
                     IETITGVKPTFVEADCSDISAMRQLFSTHPGIQGIIHFAALKAVGESVDKPLQYYHNN
                     LLSTINMLAMMNEFKVKNLVFSSSCTVYGQPDELPVTEQSPMKPALSPYGNTKKICED
                     IIRDAAAANPDIRAIALRYFNPIGAHPSVYIGEQPRGKPDNLVPFITQTAAGVRDMLQ
                     IFGGDYHSPDGTAIRDYFHVVDLAKAHILAVERLMWGKNKSNFEVFNVGSGRGVTVLE
                     IVRAFMAVTGVNVPYEVVGRRAGDIEKVWADTSLAQKELGWKPESSLEDALLSAWRWE
                     KKLRGL"
     gene            2888..4804
                     /locus_tag="PK502_09605"
     CDS             2888..4804
                     /locus_tag="PK502_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPH01113.1"
                     /translation="MKHAVLFLLGFPLPALAQPDSTSMKVNLDTVWVFSPQRVSSYSE
                     LSRMVGVVKRSELRQMKATSLSEVLERSASIDIRQRGTHGVQADINFRGGSFDQTLVM
                     LNDINISDPQTGHHNLNIPIVLDAVSRIEILQGPGARELGQGAFSGAINIITGPSAKN
                     EFSLSTFAGEYGLLKTSASATFGSAKIRTFTAASFERSNGYTDNTDFTGENFFFHTHF
                     YTGAGNFSLFSGYQDKGFGANSFYTPKYPNQFEATRTLLSGLKYSQAFGRTSISARGY
                     YRKNFDRFELFRENPPSWYMGHNYHTTDVYGLKANISMLYGFGKTEVGAEIRREEILS
                     SNLGEALSDSVRVKGRNAWYTKGGNRNLLNIFASQTFYLKRATLSGGLQLAYNSSFEY
                     IWCWGADISYSLNNHSRFFASVNRSYRVPTFTDLYYSSPTNVGNPNLKPEFATTIEGG
                     LKANRKAWSVQLAAFYRQGSRIVDWVKEPTDEAWHTINHTSLNTTGVEVFVQADLSEI
                     FRDAGSINFSYSFTSAEKNEDALLDSYYAMDYLRHNLRLALNQQFFKSLTLNLCYHFM
                     ARAGEFQDFATSQIRKYPGVHLADVRLTYSTTRKLDLFIDVSNIFNAHYMDVANVMQA
                     GRWVGAGMNLNFNL"
BASE COUNT         1282 a         1111 c         1174 g         1490 t
ORIGIN      
        1 tggcaaacaa ttccctccgt agtgggaaaa aactggctat tcgggctggg tattggcgat
       61 gtaagggaga ctctggccaa ggcctatcag gagaaaaaca tggattttgc acttaacaag
      121 cgcttaaacg cccacaacca gtatctggag acatacgtgg gtcttggatt gatagggtta
      181 agctgtttgc tgcttattct gggtagcgcc ctttggcaat cgattagaag aagggatatg
      241 gtatttttcc tgtttatgct tattattctg attaacttca tgtttgagtc agtgctggag
      301 cgattttttg gggtgctgtt ttttgtattc ttcctctcca ttttctcgat gagggaggta
      361 gattctgttt aaatttctga cgttattggg gattaaaaaa gggcttcagc tgaagccctt
      421 tttttagtaa tgttttacct tgatgtagtc aacaattttt gtcctgaact gccatagcag
      481 cgaggctata aatcctatta cagcaaatat taagccccat tttaccccgt atttggagta
      541 agggttctcc gcttttgaga gaggggtaaa atcctgaact atggtaatgg gatccttgtt
      601 gattttattc tccctttcca acctcatctt atcacgttgc agtttaagga gctggtcgtg
      661 gtatagcttt acctcctttt cgttgagaaa gaccatttgg ccgttcgatg ctttttgaag
      721 caggggtgtc tcaaagtagg ctattttttg taagctgtct attttcttta tctcgacatt
      781 tatcacccta atcatctcct cgttctgggc aatcctttcg ttgttgttct caacaacaaa
      841 ttcatttttg tagatgtact tctttaatcc ctcacgtgca gccgtgaacg ctccctcgtt
      901 gtacacctcc aagcttacaa aaaagtaatc ggtcaggcgt tttgccattg tgtcctttgg
      961 gttaaaacgg aatgagttgg cgtaatcaac gtagtcaact atcttatctc tattaaggtc
     1021 aataccataa aaggctgcaa ttgattttat ttgctttgcc ttgccaaccg gaatgtcgag
     1081 gtaggcagat gcagatgaat aatttttttc cttaaaaaga ctgttgagca ggttgatgga
     1141 gttaacgatg tggctgttat caacagaatt tgaaatggcc gtcatttccg atgagtagta
     1201 gcgggggctg cttttataga ttaagtagcc tacaagcaaa ccaataattg caaaggatac
     1261 gatccacagc gactttcgaa tgagcagaat aaataggttt tcgacctgtt tgaataacca
     1321 cacggtgccc ctcttaattc cccttcccat ccggttgaat agctcaatta ggtcaacctc
     1381 gtcggttgct ttagttttat ttacttgctc gtcgctcata tattcgtgat ttagtgattc
     1441 tcttttctgt aaatcctgat ttggcaacaa atatattact tttgttcaaa agtatctaaa
     1501 aatggacaag attcttgtta caggtggagc cgggtatatt ggttcgcaca cggttgttga
     1561 gcttgttggc gccgggtatg aagtggtgct ggttgataat ttttccaact cctcaccgga
     1621 ggtgctacag ggaatagaga cgattaccgg cgttaaacca acttttgttg aggccgattg
     1681 ctccgatatt tcggccatgc ggcagctgtt ctcaacccat cccggaattc aaggcattat
     1741 tcattttgct gcactgaagg ctgtaggcga gagtgtggat aagcctctcc aatactacca
     1801 taacaacctt ctttcgacca ttaatatgct ggccatgatg aacgaattca aggtgaaaaa
     1861 cctggtgttc tccagctcct gcacggtgta tggtcaaccc gacgagctgc ccgtaaccga
     1921 acaatcgccc atgaaaccag ctctttcgcc ctatggcaat accaaaaaga tttgcgagga
     1981 tatcattcgc gacgccgctg cggcaaatcc cgatataaga gctattgcac taaggtactt
     2041 taaccccatt ggtgcgcacc cttctgtcta cattggcgaa caacccaggg gaaaacccga
     2101 caacctggtg ccatttatca cccaaaccgc tgcaggggta agggacatgc tccaaatatt
     2161 tggaggcgac taccattccc ctgatggaac agccattcgc gactacttcc acgttgttga
     2221 cctggctaaa gcccacattc tggctgttga aaggttgatg tgggggaaaa acaagtctaa
     2281 ctttgaagtt tttaacgtgg gtagcggacg tggtgttact gttctggaaa tagttagggc
     2341 atttatggcg gttaccgggg taaacgttcc ctacgaggta gttggccgcc gtgccggtga
     2401 tatcgaaaag gtttgggccg atacttccct tgctcaaaag gagctgggtt ggaaaccgga
     2461 aagtagcctc gaggatgccc tgctctcggc atggcgctgg gagaaaaagt tgagaggatt
     2521 atagcaccct actagcctct tccggccttt tctgaggttc ataagttttt agattggacg
     2581 tgcggaagcg tccaattctt ttttaccaat cccaatacat tcttccatat taacaccagc
     2641 agcaagttga tggctttact catcatgttt attaacatag tcatagggtg atgtttacct
     2701 accggcttgt aatacttttg cagcgtgttt ttaagccata ctacaccatt gattttttgt
     2761 aagggttttc tataaaactt tgggcgtttg cctacgacac gtcctttggg gttcattcca
     2821 gcacaaacaa gtgtagggtg ttgatatata gaatattatc aattaatagc actaaaattt
     2881 tgtatggatg aagcatgccg tacttttcct gttgggattc ccgttgcctg ctttagcaca
     2941 gcccgattca acttccatga aggtaaattt ggacactgta tgggtttttt ccccgcaaag
     3001 agtttcgtcc tactcggagc ttagtcggat ggttggcgtt gtcaaaagga gcgagctgcg
     3061 ccagatgaag gcaacttccc tttcggaagt gctggaacgc tccgcatcta ttgatattcg
     3121 tcagcggggt actcacggtg tgcaggccga tattaacttt cggggaggat cgtttgacca
     3181 gacgcttgtc atgctcaacg atataaacat cagcgatcca caaaccggtc accataacct
     3241 gaatatcccc atcgttctcg atgcggttag tcgtattgaa atacttcaag gccccggggc
     3301 ccgggagttg ggacagggcg ctttttcagg agccatcaat ataatcacag ggccttcggc
     3361 aaaaaacgag ttctcacttt caacttttgc cggtgagtac ggcttgctga aaacctctgc
     3421 tagcgcaaca tttggttcag caaaaatacg aacctttact gctgcctcct tcgaaaggag
     3481 taatggctac accgataata ccgatttcac cggggagaac ttttttttcc atactcattt
     3541 ttacaccggc gctggcaact tttccctatt ttcgggttac caggataagg gttttggggc
     3601 taatagcttc tacaccccaa aataccccaa ccagtttgag gctacaagaa cgctgctaag
     3661 cgggcttaag tacagtcagg cttttggtcg aacctcaatt tcagccagag gttactaccg
     3721 taagaatttt gacaggtttg agctttttcg tgagaatcct ccttcgtggt acatggggca
     3781 taactaccac acaaccgatg tttacgggtt aaaggcaaac atctcaatgc tgtatggatt
     3841 tggcaaaacc gaagttggag ccgaaatcag gagggaggag attcttagca gcaacctggg
     3901 tgaggcactt tcagattcgg tgagggtgaa aggccgtaat gcctggtaca ccaagggtgg
     3961 aaatcgaaat ttattgaata tttttgcctc ccaaaccttt tacttaaaaa gggctaccct
     4021 gtcgggtgga ctgcagctgg cttacaacag tagtttcgaa tacatttggt gctggggtgc
     4081 cgatatttcc tactcgctga ataaccattc caggtttttt gcctcggtca accgctcata
     4141 cagggtacca acctttaccg atttatacta cagcagtcct accaatgttg ggaatccaaa
     4201 ccttaaacct gagtttgcca caaccattga gggcgggcta aaggccaata ggaaagcgtg
     4261 gtcggtgcag cttgccgcat tctaccgaca gggaagcaga atagttgact gggtgaagga
     4321 gcccaccgac gaggcgtggc ataccattaa ccatactagc ctgaacacaa ctggcgtgga
     4381 agttttcgta caggctgatt tgagtgaaat ttttagggat gccggtagca taaatttcag
     4441 ctactccttt accagcgctg aaaagaacga ggatgctctg ctcgactcat actatgcaat
     4501 ggattaccta cggcataacc tgaggttggc tttaaaccag caatttttta agagccttac
     4561 gctaaacctc tgctaccatt ttatggccag ggcaggagag tttcaggatt tcgccacctc
     4621 gcaaatcagg aagtaccccg gtgttcatct ggccgatgtt aggcttacct acagcaccac
     4681 gagaaagctt gatctgttta ttgatgtttc gaatattttt aacgcccact atatggacgt
     4741 tgccaacgta atgcaggccg gacgttgggt aggtgcaggc atgaacctaa atttcaactt
     4801 gtagtactgt tttaaatgca atcaaagttg aacatgcggt attttgaatt ggcctgcgat
     4861 gtttttcttt aaaaattccc agtgagtttt gaaaggtgtt cttaaattaa ggtacccgca
     4921 tgataacccc gctaaccacc ttacctgttt catagttgag ctccgaccct ttagtgctat
     4981 gaggaattga gaaaaccact atcgtaacaa ttgctgcagc aattactatc ttgggtcggg
     5041 agtgcttgcg gttggct
//
LOCUS       DAOPHX010000153         5038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_22193_length_5038_cov_6.690749, whole genome shotgun sequence.
ACCESSION   DAOPHX010000153 DAOPHX010000000
VERSION     DAOPHX010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22193_length_5038_cov_6.690749"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..1205)
                     /locus_tag="PK502_09610"
     CDS             complement(222..1205)
                     /locus_tag="PK502_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPH01114.1"
                     /translation="MNSFGRIFRVSLFGESHGRAIGVVVDGVKPGLPLSTDDFADDIR
                     RRGPGLPGTTPRTENDLPEMVSGWFNGYTTGAPLAIIFRNTNVRSDDYERIKDLPRPG
                     HADFVAYKKFSGYNDYRGGGHFSGRITLALVAAGVIAKKMIDPVEVHAKLVTVGGSTD
                     IENTINKAMSANDSVGGLVECRVSGIPIGWGEPFFDSVESVISHLAFAIPGVKGIEFG
                     SGFAAASMFGSAHNDAFIDASGATSSNNAGGILGGISNGNELQFRVAVKPTSSIAREQ
                     QTINMRTGMPEKLVVTGRHDTCIALRVPVVLEAVAAIALADLAAIQNKSKG"
     gene            complement(1221..2477)
                     /gene="aroA"
                     /locus_tag="PK502_09615"
     CDS             complement(1221..2477)
                     /gene="aroA"
                     /locus_tag="PK502_09615"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPH01115.1"
                     /translation="MRRLVKPSLVSGAVQAPPSKSVAQRAIAMATLANGVTEIYNPGN
                     SNDSKAAVEICRALGASIAEYPGKLVVTGGLQQPSQILNCGESGLGIRMFSAIASTFS
                     AGVTLTGTGTLLKRPMQPIADALVQLGAACSTSSGFLPITVKGPINGGVANIDGSQGS
                     QVLTGLLVASPLAKHDVTIRATKLKSVPYVHLTIQMMHDFGVEVDVGSDIDFHVRAKQ
                     RYAPCTYNVEGDWSGTAFMLVAGATAGEVEVKNLNPSSLQADRAIVDALVMAGATVDV
                     EKNGVYVKRNKLSHFEFDATNCPDLFPPLVALASHCDGESKILGVSRLRVKESDRAAT
                     LVEEFAKLGIDIKIKDDLMVVQGGKVKSAWVHSHGDHRIAMACAVAALSGEGNLTIEG
                     AESVSKSYPRFFDDLNSIMTNSSLNV"
     gene            complement(2446..3381)
                     /locus_tag="PK502_09620"
     CDS             complement(2446..3381)
                     /locus_tag="PK502_09620"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPH01116.1"
                     /translation="MTRKVYAVFGNPVLHSCSPQLFNSVFSEIPVEAYYTRIRPANAA
                     NIPELIRHLNLAGANITTPFKTEVMTHLDWLSDEAEIIGGVNTIINNQGYLKGYNTDY
                     LGVSRSLQEAGLHLQGSRCLVLGAGPAACAAVYALINEGADVTVVNRTNHKAKEIANR
                     FGCSSADMDKLDSAILECDILVSALLPEANPLANIKVPEWITAIDANYRASTLSQHLA
                     LSGCRTITGKRWLLHQAAASFNLFLNFNPKIELMAKGLEAQPQREAARYQMIGEDTLR
                     SIHTEDFDMLVSVPDSSTMTFRTILDEEISKAFVG"
     gene            complement(3374..4030)
                     /locus_tag="PK502_09625"
     CDS             complement(3374..4030)
                     /locus_tag="PK502_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HPH01117.1"
                     /translation="MKILHPDICLSIGNMRYEDIFPYLKVAALAEIRLDLLNLTDEQI
                     RGLFTAHGNLIATYRCNDSAKMFQTLTNTLDSGAAFVDVDCLTENSIRDAIVAKAKGL
                     NRRVIISYHNFECTPNKEELERIIYSMKSQGADICKVACMANEPLDCSRVMALYEHIP
                     DLVAFCMGEAGKITRLAAPILGAPFTYASVAGASTAPGQLDYLKMEEYLNYFLPLNHD
                     "
     gene            complement(4106..>5038)
                     /gene="aroB"
                     /locus_tag="PK502_09630"
     CDS             complement(4106..>5038)
                     /gene="aroB"
                     /locus_tag="PK502_09630"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPH01118.1"
                     /translation="IITDSNIDRIYGNRLPPLPKFVVNPGEQSKTLLRLTEIYRWLLD
                     SGADRNSFIVGIGGGVVCDITGFVASTYMRGVRFGFVATSLLAQVDASIGGKNGVNLD
                     GYKNIVGTFNQPQFVICDTSLLKTLPETELSNGFAEMVKHALIADAPQFTILESNTNR
                     LNTCDLELLDSLVASSVGVKASIVTADERESGERKKLNFGHTWGHAVEKVTGIPHGQA
                     VSIGMAFASQLSAEKGLLTPDECHRITSLLIALNLPVSIKANTNNIFEALQRDKKRCS
                     NAIDFILLEGIGKAVIKSLSLVEIKEFIERKYCY"
BASE COUNT         1231 a         1274 c         1185 g         1348 t
ORIGIN      
        1 ctccatacaa ggggctgcgc cccttgctac ttatttcgcc ccttcggggc ttaaatccgc
       61 cggtaggcgg acaagttcca tcaggttacc gacaagttcc gccggtagtc gagcaagttc
      121 cggaatcttg aatccgccag taggcggaca ggttctggaa tccgccagta ggcggacagg
      181 ttctagaatc ttgaatccgt caggagccgg acaagtttat attatccttt actcttgttt
      241 tgaatggcgg ccaggtcggc aagggcaatg gctgctacag cttccagcac aaccggaacc
      301 cttagtgcta tgcaggtgtc atggcgtcct gtaaccacta atttctctgg catccctgtc
      361 ctcatgttaa tggtttgttg ctctctggct atgctcgatg tgggcttaac tgccacccta
      421 aactggagct cgtttccgtt gcttatgccg cctaaaatgc ctccagcatt gttggatgag
      481 gttgctcccg aggcatcaat aaacgcatcg ttatgggcag aaccaaacat ggaggcggct
      541 gcaaaccctg agccaaattc aataccctta acccccggaa tggcgaatgc caaatggctt
      601 attaccgatt ccaccgaatc aaagaatggc tcgccccaac cgataggtat gccggaaacc
      661 ctgcactcta caagcccccc aaccgaatcg ttagccgaca tggccttgtt tatggtattt
      721 tcgatatcgg tgcttccgcc aactgtgacc aatttggcat gaacctccac cgggtcaatc
      781 attttttttg caataacgcc tgccgcaacc agcgcaagag tgattctacc agagaaatga
      841 ccacctccac gatagtcatt gtacccacta aattttttgt aggcaacaaa atcggcatga
      901 cccgggcgag gtaagtcctt aatgcgttcg tagtcgtctg accgtacgtt ggtatttctg
      961 aaaataattg ccagcggtgc tcctgtggta tagccgttga accaacctga caccatttcg
     1021 ggtaaatcat tctcggttct gggggttgtc cctggtagtc ccggcccacg ccgacgtatg
     1081 tcgtcagcaa aatcatcggt tgataggggc agccctggct ttaccccatc aactactacg
     1141 ccaattgccc ttccgtgcga ttcgccaaat agcgatactc tgaatatcct accaaatgaa
     1201 ttcactggat tataggttag ttaaacatta agcgagctat tagtcattat gctgttcaga
     1261 tcgtcgaaaa aacgggggta gctcttcgat actgactcgg ctccctcgat ggtcagattt
     1321 ccttcacccg aaagtgcagc aactgcacag gccattgcaa tgcgatggtc gccgtgcgag
     1381 tgaacccatg ctgatttcac ctttcctccc tgaacaacca tcaaatcgtc cttaatcttg
     1441 atgtcaatac ccagcttggc aaattcctcc accagggttg cagccctgtc gctttctttt
     1501 acccttaatc ggctgacgcc taaaatttta gattcgccat cgcagtgtga ggcaagtgca
     1561 accagtgggg gaaacaggtc aggacagttg gtggcatcga attcaaaatg gctgagcttg
     1621 ttacgtttaa cataaacgcc atttttttca acatccacag tagcgccggc catcacaagg
     1681 gcatcaacaa ttgccctatc ggcctgcagc gacgaggggt taagattttt cacctctact
     1741 tcgcctgccg tagcaccggc aacaagcatg aatgcagttc cgctccaatc accctcaacg
     1801 ttgtaggtgc agggagcata acgctgtttt gcccttacgt gaaaatcaat atccgatcca
     1861 acatctacct caaccccaaa atcgtgcatc atttgaatgg tgaggtgcac atagggaaca
     1921 cttttgagtt tcgttgccct tatggtaaca tcgtgtttgg ccagtggtga agccacgagt
     1981 agcccggtga gcacctgcga gccttgcgag ccatctatgt tggctactcc gccattaatt
     2041 gggcctttca cggttatggg tagaaatccg gatgaggtgc tgcaggcagc tccaagttga
     2101 accagcgcat cagcaatggg ttgcataggc ctttttagga gcgttccggt tccggttaag
     2161 gtaacgccgg cactaaatgt tgaggcaatt gccgaaaaca ttctaatacc caaaccggat
     2221 tcgccacaat taaggatttg ggagggttgc tgcaatccgc ccgttaccac cagtttaccg
     2281 gggtattcag ctatgctggc gccaagcgcc cggcaaatct caactgctgc cttagaatcg
     2341 ttactgttgc caggattgta tatttccgta acgccattgg ctaaagttgc catggctatt
     2401 gccctttggg caacgctttt cgaggggggg gcctgcacag ctccgctaac caacgaaggc
     2461 tttactaatc tcctcatcta aaattgttct gaatgtcatg gttgagctgt cgggaaccga
     2521 tacgagcatg tcgaaatctt ccgtatgaat cgaacggagt gtgtcttctc ctatcatttg
     2581 gtacctagca gcttcccttt ggggttgtgc ctcaagtcct tttgccatta gctcaatttt
     2641 cgggttgaaa tttagaaaaa ggttgaatga ggctgctgcc tggtgtagca gccagcgttt
     2701 acccgtaatc gttctgcagc cgcttaatgc caggtgttgt gatagcgttg aggcacggta
     2761 gttggcatca attgccgtaa tccactccgg aaccttaatg tttgcaagtg gatttgcctc
     2821 gggaagaagc gccgaaacca gaatatcgca ttcgagaatg gcgctgtcca gtttatccat
     2881 atcagctgag ctacagccaa accgatttgc tatttctttt gctttgtggt ttgttctgtt
     2941 aacaaccgtt acgtctgcgc cctcattaat caaggcataa actgcagcac atgcagcagg
     3001 ccctgcgccc aaaacaaggc atcggctacc ctgaagatga aggcctgctt cttgaagcga
     3061 acgactcaca cccaggtagt cggtgttgta ccccttcagg taaccctggt tgttaattat
     3121 ggtatttaca ccacctatga tttctgcttc gtccgaaagc caatcgaggt gcgtcattac
     3181 ttcggtttta aaaggcgtgg tgatgtttgc accagccaga ttcaagtgcc ttatcaactc
     3241 gggaatgttg gctgcatttg caggtcggat acgggtgtag taggcttcaa cgggtatttc
     3301 gctgaaaacc gagttgaaaa gctgggggct gcagctgtgg agcacaggat tcccaaaaac
     3361 ggcatacacc ttcctagtca tggtttaacg gtaaaaaata attcaggtat tcctccattt
     3421 tcaagtagtc gagttgcccg ggagcagttg atgcgccggc aaccgaagcg taggtaaatg
     3481 gtgcgcccag aatcggagcg gctagccggg taatttttcc tgcttcaccc atacaaaaag
     3541 ctactaaatc cggtatgtgc tcgtacaaag ccatcaccct tgagcaatca agcggttcat
     3601 tggccatgca tgcaacctta caaatatcgg caccctggct tttcatgctg taaattatcc
     3661 tttcgagttc ttccttgttg ggtgtgcatt caaagttgtg gtatgagata ataacccttc
     3721 ggtttagccc tttagccttg gcaacaatgg catctctgat ggaattttct gtaaggcagt
     3781 caacatcaac aaaggccgca cctgaatcga gtgtattggt aagtgtttga aacattttag
     3841 cgctgtcatt gcacctataa gttgcaatca ggttcccatg tgcagtaaaa agccctcgta
     3901 tctgttcatc tgtaagattc aggaggtcga gccgaatttc ggcaagggca gctaccttca
     3961 ggtaggggaa aatatcctca tacctcatgt ttcctatgct caagcaaata tcggggtgca
     4021 aaatcttcat agccgttttc tcatcagtaa tatgctgtcg caacttgtat aaaaagcagg
     4081 aatagtcaac ttttaacttt tcttttcagt aacaatactt cctttctata aactccttaa
     4141 tctcaacaag gcttaggctt ttgataacgg cctttccaat gccctcaagc aggatgaagt
     4201 ctatagcgtt ggagcatcgt tttttatccc tctgcaatgc ctcaaaaata ttgttggtgt
     4261 tggcttttat ggaaacaggt agattaagcg caataagcaa gctggttatt cgatggcatt
     4321 catctggtgt gagcaaccct ttttcggccg agagctgcga ggcgaatgcc attcctatgc
     4381 taacggcttg tccgtggggg atccctgtta ccttttcaac tgcatggccc catgtgtggc
     4441 caaagttaag cttctttcgt tcgccgctct ctctctcatc ggcggttaca attgatgcct
     4501 taaccccaac cgatgaggca accaacgagt cgagtaattc taaatcacag gtgttaagcc
     4561 tattagtgtt actttccaag atggtaaatt ggggagcatc ggcaatcagg gcatgtttta
     4621 ccatttcggc aaaaccgttg gacagctcgg tttcgggtaa cgttttaaga agcgaggtat
     4681 cacaaatcac gaattgcggc tggttgaatg ttcccacaat gttcttatag ccatcgagat
     4741 tcactccatt cttaccacca atgcttgcgt caacctgggc gagcaggctg gtggccacga
     4801 agccaaacct aaccccccgc atgtaggtag aggccacaaa accggtaata tcgcaaacaa
     4861 ccccgccgcc aatcccaacg ataaacgagt tcctgtcggc cccagagtcg agcaaccacc
     4921 ggtaaatttc ggtcaagcgg agaagggttt tggactgctc gccgggattt accacaaatt
     4981 tagggagtgg cggcaacctg ttgccgtaaa tcctgtcaat gttgctgtcg gtaataat
//
LOCUS       DAOPHX010000154         5037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_22198_length_5037_cov_5.435769, whole genome shotgun sequence.
ACCESSION   DAOPHX010000154 DAOPHX010000000
VERSION     DAOPHX010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22198_length_5037_cov_5.435769"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..1220
                     /locus_tag="PK502_09635"
     CDS             9..1220
                     /locus_tag="PK502_09635"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPH01119.1"
                     /translation="MIILVLNCGSSSIKYQLINMANESQLLAKGIVERVGFKDAILTH
                     KPVGKDKYEMVASIPDHTTGINLILEALVDKNHGVVESLSQIKAVGHRVAHGGEFFTK
                     SCLIDSNTKREIEKCIELAPLHNPANLKGILSMEKLLPNVPQVAVFDTSFHQTMPKEA
                     YLYALPYEYYENYKIRRYGFHGTSHMFVAKKACKILGADFDQLRIITCHLGNGASIAA
                     IKNGKSIDTSMGFTPVEGLIMGTRSGDIDPGILLFIAEKENLSIQQVNDVINKKSGVF
                     GISGISSDMRDLEDAAASGNERARLALDMYYYRVKKYIGSYAAAMDGVDLIIFTGGIG
                     ENDSTLREKVCGALDFMGVEFDVEANRGVRGQDKLLTKPNSLVKVMAITTNEELVIAT
                     DTANIINNLKN"
     gene            1256..2161
                     /locus_tag="PK502_09640"
     CDS             1256..2161
                     /locus_tag="PK502_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPH01120.1"
                     /translation="MRVDKLDQLFDLVKSKPRRRLVAAFANDAHTIEAVSLAVDRGIV
                     DATLVCDEPVMKKVCAEHNIDPGKFRIVQEADEMKAARRAVELINRGEGDVLMKGLVS
                     TDKYMRAILDKENGLLPPKAVLSHVTVVEVPTYPKLLIVGDVAIIPAPDLNQKIAIVN
                     YLIQTAHSLGIEKPKVALNAATEQMLAGMPACVDAAIISKMADRGQIKGAIVDGPLAL
                     DVAIDMESAQIKKLGGGVAGDADCILFPNIEAGNVFFKSCTKLANGELGAMVMGAKVP
                     CVLTSRGDSIKSKMYSIALAANAVK"
     gene            2189..3265
                     /gene="buk"
                     /locus_tag="PK502_09645"
     CDS             2189..3265
                     /gene="buk"
                     /locus_tag="PK502_09645"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPH01121.1"
                     /translation="MDEFKILAINPGSTSTKIAVYNNNKSIFLKSIKHSVEDLAQFKT
                     ITDQFDYRKKIILEELGQTGIDVNKISAVVGRGGLLKPIESGVYEVNEQMKSDLRSCI
                     MGEHASNLGALIADDIAQSLPNAKAFIADPVVVDELNDVARISGHPLFKRVSIFHALN
                     QKAIARLHAKSLNKPYEELNLIVAHLGGGISVGAHHNGRVVDVNNALDGDGPFSPERA
                     GTLPAGQLAKLCYSGQYSYDEVKKMITGKGGLVAHTGTNNAYEIEVKARGGDAMAKLL
                     QDAMAYNVGKAIGEMATVLKGKVDSIVLTGGIAHNPDLVEYIKEMVGFIAPIAVYPGE
                     DEMQALAMNALMVLKGEVIPKIYR"
     gene            complement(3475..4182)
                     /locus_tag="PK502_09650"
     CDS             complement(3475..4182)
                     /locus_tag="PK502_09650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPH01122.1"
                     /translation="MNIIVTGASRGIGYQTVLQLAKSAGNRIIALARDHEQLTRLQLE
                     SRKVQPESEVIPLVFDLQQTSSYDDALIPQIKGHFTSADILINNAGLLVNRTFEQLTL
                     LEMVQMLTVNFIAPAYLTKLIIPLMTKGGHVVNISSMGGFQGSQKFKGLSIYSASKAA
                     LASLSECLAEEYINKGISFNCLALGATDTEMMRAAFPGYNAPIKPNEMAKFIAEFAIK
                     GNQYFNGKIIPVSTSTP"
     gene            4350..4982
                     /locus_tag="PK502_09655"
     CDS             4350..4982
                     /locus_tag="PK502_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01123.1"
                     /translation="MRKIIANLTKHSIKILSYLLVATGVMLIGSARAQDQEQGRNFDR
                     FSDRLFFGGSFGLAVGSRVTQVDVAPMMGIWIFPEWSLGLGGRYSYRRERFSINGFTT
                     EPIETHVWGVSGFTQVIPITDFHRTFGIDLHGGIILHGEFESLYLDRNFFDQSSSYGK
                     GWVNMYLVGGGWHQPIGDRAAINFLVLWDLTDNRYSPYNSNPILRFTITF"
BASE COUNT         1420 a         1011 c         1179 g         1427 t
ORIGIN      
        1 taacaaaaat gattatttta gttttgaact gcggaagttc gtcaatcaag taccagctga
       61 ttaacatggc taacgaaagc cagctgttag ctaagggtat tgtagaacgt gttggtttta
      121 aggatgccat tcttacccac aaaccagttg gaaaggataa gtatgaaatg gtggcttcca
      181 ttccggatca tacaaccggc attaatctca ttttagaggc acttgttgac aaaaatcatg
      241 gagtggtaga aagcttaagt cagataaaag cggttggcca cagggtggct catggtggtg
      301 agttttttac caagagctgc ctgatagata gtaacacgaa aagggagata gagaagtgta
      361 ttgagttggc tcccctgcac aaccctgcaa acttaaaggg tattctttca atggagaagc
      421 ttttgccaaa tgtgcctcag gttgctgtgt ttgatacatc ctttcaccaa accatgccca
      481 aggaagccta cctttacgct cttccctacg agtactatga aaactataaa attcgcaggt
      541 atggatttca tggcaccagc cacatgtttg ttgcaaaaaa agcttgtaaa attttaggag
      601 ccgactttga ccagctacga attattacat gccatttggg gaatggtgct tcaattgcag
      661 ccatcaagaa tggcaaatct attgatacct caatgggatt tactcctgtg gaaggcttaa
      721 taatggggac acgaagcggc gatattgatc cgggcatctt gctgtttatt gccgaaaagg
      781 aaaatctttc cattcagcag gttaatgatg taatcaataa aaagagcggc gtttttggaa
      841 tttccggaat atcctccgac atgcgcgacc ttgaggatgc ggctgcaagc ggaaacgaac
      901 gtgcacgcct ggcgctggat atgtactact acagggtaaa aaaatacata ggctcgtatg
      961 cagcagcaat ggatggtgtt gatttaatca tctttacggg aggaattggt gaaaatgaca
     1021 gcactcttcg cgaaaaggta tgcggtgccc ttgattttat gggggttgaa tttgatgtag
     1081 aggcaaatag gggcgttcgt ggacaggata agctacttac aaaacctaac tcccttgtaa
     1141 aggttatggc tattacaacc aacgaagagc ttgtgattgc aactgatact gcaaatatta
     1201 tcaataactt gaaaaactag aacaattaat tttaactata gaggagatta ggaatatgag
     1261 agtagataag cttgaccaac ttttcgattt ggtaaaatca aagccccgaa gaagacttgt
     1321 tgctgcattt gccaacgatg cccacactat tgaggcagtt agcctggctg ttgatagggg
     1381 tattgttgat gccactcttg tttgcgatga gcctgttatg aaaaaggtgt gcgctgagca
     1441 caacatagat ccgggcaagt tccgtattgt tcaggaagcc gacgaaatga aggctgcgcg
     1501 tagggcagta gaacttataa acagggggga aggcgatgtg ctgatgaaag gactggttag
     1561 caccgataag tatatgcgcg ctatactcga caaggaaaat ggattgctcc cacccaaggc
     1621 tgtattaagc catgttaccg ttgtggaggt tcctacttat cccaagctgc tcattgttgg
     1681 cgatgttgcc attattccag ctcccgacct gaaccagaaa atagccatag taaactacct
     1741 tattcaaact gcccattccc ttggaattga aaaacccaag gtagctttaa atgctgctac
     1801 cgagcaaatg cttgccggta tgccggcatg cgttgatgct gccattatct ccaaaatggc
     1861 cgatagggga caaattaagg gtgctattgt ggatggccca ttggcgcttg atgttgccat
     1921 tgatatggag tcggctcaaa taaaaaaact gggtggcgga gttgcaggtg atgccgattg
     1981 tattcttttc cccaacattg aggcaggcaa tgttttcttc aaatcgtgca caaaacttgc
     2041 taatggtgag cttggagcta tggttatggg ggcaaaagtt ccatgcgtcc ttacttcacg
     2101 tggcgatagc attaagtcga aaatgtactc gattgccctt gctgctaatg cagttaagta
     2161 gttccccact accaaattaa cttgtgcaat ggatgaattt aagattttag ccattaaccc
     2221 cggctcaacc tcaacaaaaa ttgctgttta caacaacaac aagagcatct ttctcaagag
     2281 catcaagcac tcggtagagg atttggctca attcaaaact attacagacc agtttgacta
     2341 ccgaaagaaa attatactcg aagaattagg ccaaactgga attgacgtta ataagatatc
     2401 cgcagtagta ggccgcggtg gactgcttaa accaatagaa tcaggggttt atgaagtaaa
     2461 cgagcagatg aaatccgatt tgcgcagctg tatcatgggt gagcatgcaa gcaacttagg
     2521 cgcactaatc gccgatgata tagcccaatc gcttccaaat gctaaagcat ttattgccga
     2581 ccctgttgtt gttgatgagc ttaacgatgt ggctcgtatt tcggggcacc ccctctttaa
     2641 aagggtatcc atttttcatg ccctaaacca gaaggcaata gcacgccttc atgccaaatc
     2701 acttaacaag ccctatgagg agctgaactt gattgttgct cacctgggag gcggtatctc
     2761 tgtaggtgcc catcataacg gaagggttgt tgatgtgaat aacgcactgg acggcgacgg
     2821 gcctttctcg cccgagcgcg caggaacact tcctgctggc cagctggcaa aactatgcta
     2881 tagcggccaa tactcctacg acgaagttaa aaaaatgatc accggaaaag gtggactagt
     2941 cgctcacacg ggcactaata atgcctatga aattgaggtt aaagctcgag gtggcgatgc
     3001 tatggctaag ttgctacagg atgccatggc atataatgtt ggtaaggcta ttggcgaaat
     3061 ggctaccgtg ttgaaaggga aagttgactc cattgttctg accggtggca tagcgcataa
     3121 ccctgacttg gttgaataca taaaggaaat ggttggtttt attgcaccta ttgccgttta
     3181 ccccggagag gatgaaatgc aagcccttgc catgaatgcc ctaatggtgc taaaagggga
     3241 ggtaattccc aaaatttaca ggtaggtaac agcagaatct actactattc gcttaaagtt
     3301 aagggagact tgtgatggtc tctctttttt tatcgttgta ctttgcacca tacacgtagt
     3361 tgacttgcga ataattgaat gtattgtttt acctgtatta ttcgtaagct atatcggcat
     3421 tggaagtaaa tgtagtaagt tgattattaa tatgcagtga aaataatgta atgactatgg
     3481 ggttgaagtc gagactggaa taattttccc gttaaagtac tggttacctt taattgcaaa
     3541 ttcggcaata aatttggcca tttcattcgg cttaatagga gcattatagc caggaaatgc
     3601 agcgcgcatc atttctgtgt cggttgctcc tagcgccaag caattaaacg aaataccttt
     3661 atttatatac tcctccgcca ggcattccga tagcgacgcc agcgcagctt tagaagcgga
     3721 gtatatggaa agacctttaa acttctggct tccctgaaaa cctcccatac tgctaatgtt
     3781 cactacatgt ccgccttttg tcattaatgg aataatcaat tttgtcaagt aggctggcgc
     3841 tataaagttt acggtcagca tttgcaccat ttccaaaaga gtaagctgct caaatgtcct
     3901 gtttacaagc aacccagcat tgtttattag aatatcggca gaagtaaaat gtcctttgat
     3961 ttgaggaatc aatgcgtcat cgtaactgct agtctgctgt aaatcaaaga ctaagggtat
     4021 tacctccgat tcaggctgca cctttctgct ctctagctgc agccttgtta gctgttcatg
     4081 gtccctggct agagctatga ttctgtttcc tgccgatttc gcaagctgca gtactgtttg
     4141 gtatccaata cctctggagg ctccggttac aataatattc ataatttttt gtttactaac
     4201 cttttttctt tcattttgtt ttgataaaat tgcacgagca ggcagatttc cgttccgtat
     4261 atacggcatt accttgaccc tatcctgggt ggctaattaa aaaagggaag gtaatacttt
     4321 atttcataaa cctgattagt gttgtccgta tgagaaaaat aattgccaac ttaaccaaac
     4381 attcaattaa aattctttcc tatctcctcg tggccacagg agttatgttg ataggttcag
     4441 caagagcgca ggaccaggaa cagggtagaa attttgaccg attttccgat aggctatttt
     4501 ttgggggaag ttttggattg gctgttggta gcagggttac gcaggttgat gttgccccca
     4561 tgatgggtat atggattttc ccggagtggt ctttagggtt aggcgggcgc tacagctacc
     4621 gtcgtgaacg ctttagcatt aatgggttca ccaccgaacc tatagagaca cacgtttggg
     4681 gagtgtcggg atttacccag gttattccta ttaccgattt ccataggact tttggcattg
     4741 atctgcatgg gggaattatt ttgcatggtg aatttgaaag tctttacctc gaccgaaact
     4801 tttttgacca gagtagcagc tatgggaaag gctgggtgaa tatgtacctg gttggaggag
     4861 gttggcacca acccattggc gatagggctg ccattaactt tttagtgctg tgggatttga
     4921 ccgacaaccg gtattcaccc tacaacagca atcccatttt gcgatttacc attaccttct
     4981 aataaaaaaa ggcctccatt gaggccttac tggctagtat gttaggaata aacttat
//
LOCUS       DAOPHX010000155         5018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_22330_length_5018_cov_5.302841, whole genome shotgun sequence.
ACCESSION   DAOPHX010000155 DAOPHX010000000
VERSION     DAOPHX010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22330_length_5018_cov_5.302841"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..659)
                     /locus_tag="PK502_09660"
     CDS             complement(84..659)
                     /locus_tag="PK502_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex protein RnfA"
                     /protein_id="HPH01124.1"
                     /translation="MEYLIIIIAAIFVNNVVLSQFLGICPFLGVSSKINTSVGMGGAV
                     VFVMVLSTIVTYLLQQYVLNPLNIGYMQTIVFILVIAALVQMVEIILKKISPSLYQAL
                     GIFLPLISTNCAVLGTAILVIKHNYNLLEGVVFTAATAVGFGFALVIMAGIREQMELA
                     NIPKGMKGIPIALLTAGILSLTFMGFAGIVK"
     gene            complement(669..1250)
                     /locus_tag="PK502_09665"
     CDS             complement(669..1250)
                     /locus_tag="PK502_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HPH01125.1"
                     /translation="MSAWSNLTRGIIRENPTFVIVLGMCPTLATTTSAINGLGMGLAT
                     TFVLLGSNVVISMIARFIPDKVRIPSYIVVIATFVTIVDLLMQAYTQSLYETLGIFIP
                     LIVVNCIVLGRAEAFARKNSVLDSAMDGLGMGIGFTMALTLLGSIREMLGNGSLFNYK
                     FIPGDAILVFILSPGAFIALAYLIALFNKFVKK"
     gene            complement(1247..1825)
                     /locus_tag="PK502_09670"
     CDS             complement(1247..1825)
                     /locus_tag="PK502_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HPH01126.1"
                     /translation="MSGVESSFKNMTISLLAITLVASAGLGLVYKSTKDPIAAAQVAK
                     VNNAIKAVVPEFDNQPSNEAYTVKADGDDLFFYPASKNGQLVGVAVKTFSNNGFGGRI
                     ELMVGFLPDGTINKITVISHKETPGLGDKIDPGKSDFSVQFEGKNPETFKVMVKKDGG
                     DVDAITASTISSRAFCDAVNRAYKAFKEGGRK"
     gene            complement(1850..2854)
                     /locus_tag="PK502_09675"
     CDS             complement(1850..2854)
                     /locus_tag="PK502_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPH01127.1"
                     /translation="MDNRFVVSPSPHVHSGKSTSQLMLNVVLALMPAFAVSIYYFGVG
                     AIIVTLISVLSCVLFEYLIQKFIFKVKPTISDCSAVVTGLLLAFNVPSNLPWYIIVLG
                     SLFAIGVAKMSFGGLGNNIFNPAIAARIFLLISFPVQMTSWPLPVTSRLQYIDAITGP
                     TPLAIIKEGVKNGQTISQMADSIPSHMQLFMGLMGGSLGEIAAFALLIGFAWLLITRV
                     ITWHIPVSIFTTIFIFTGILWLINPGIYADPVFHLLTGGVMLGAIFMATDYVTSPMHP
                     KGMWIYGIGIGALTVIIRVWGSYPEGVSFAILIMNAFVPLINRFVKPKRFGAAAISGK
                     "
     gene            complement(2892..4223)
                     /gene="rsxC"
                     /locus_tag="PK502_09680"
     CDS             complement(2892..4223)
                     /gene="rsxC"
                     /locus_tag="PK502_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPH01128.1"
                     /translation="MLKTFKKGGVHPPENKISAAQPVEVLPLPAKVIIPLSQHIGAPA
                     EAMVAKGDRVKTGQLIAKSAGFVSANVHSTVSGIVESLEPVMDSSGYKRPAITINVEG
                     DDWEPSVDRSPNLKKEITLSAQDIIKKIAEAGIVGLGGATFPTHVKLSVPAGKKAEML
                     VINGVECEPFLTSDHRLMLEKGHELMIGIQILMKALNVPKAMVGIEANKPDAVSHLQS
                     LAKSYSGIEVQPLKVKYPQGGEKQLIKALTGREVPSGGLPIDIGAVVHNVGTALAVYE
                     AVQKNKPLVERIVTVTGKNLAHPSNFLVRIGTPVSALIAAAGGLPDDTGKVVSGGPMM
                     GKAVSSIDVPVTKGTSGILVIPAAESKRKPEGVCIRCAKCVSVCPMGLEPYLLYRLGQ
                     RMQYSQMEVERVLDCMECGSCSFECPANLFLLDFIRLGKVEVGKIVRERKN"
     gene            complement(4266..>5018)
                     /locus_tag="PK502_09685"
     CDS             complement(4266..>5018)
                     /locus_tag="PK502_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HPH01129.1"
                     /translation="DEVEALLPGANCGGCGYPGCRGLADALVKADDISAIFCPVGGNE
                     TMRKVAAILGKAVEDKDPQIAVVRCSGSPQNRTRVVEYNGISSCKFASTLYSGDTGCQ
                     FGCLGLGDCVAVCKFDAIYIDPVSLLPVIDEEKCTACGACVKACPKLLIELRKKGPKG
                     KRIFVSCMNTDKGGVARKYCSVACIGCFKCVKVCPYEAITLENNLAYIDANKCKLCRK
                     CVEECPTDSILEVNFPPRKPKVEEQAQNAVTS"
BASE COUNT         1395 a         1279 c         1097 g         1247 t
ORIGIN      
        1 gggcaacaga agttaaatga cactcaatgc caagctttta cctttaacca aattgggttc
       61 gagggaatgc agcttgtccc accctacttg actattccgg caaatcccat gaatgtaagc
      121 gaaagaattc ctgctgtaag cagcgcaatg ggaattccct tcataccctt agggatattg
      181 gcaagctcca tctgctcgcg aatgccggcc ataattacca gggcaaaacc aaaaccgaca
      241 gcggttgcag ccgtaaaaac aactccttcg agcaggttat agttatgctt tatgaccagt
      301 attgctgttc ccagcactgc gcagttggtt gaaatgagcg gtaggaaaat tcccagcgcc
      361 tggtataggg aaggactaat cttttttaga attatttcca ccatttgaac cagggcggca
      421 ataaccagaa tgaaaacaat ggtttgcata taccctatgt tcaaggggtt taacacgtat
      481 tgctgaagga gataggttac aatggttgac agcaccatga caaacaccac ggctcccccc
      541 attccaactg acgtgttgat ttttgatgaa acccccagga atgggcaaat tcctaaaaat
      601 tgcgacagta caacgttatt gacaaatatt gcagcaataa taattatcag gtattccata
      661 acagcctcct actttttaac aaacttgttg aatagggcaa tcaggtatgc cagggcaata
      721 aatgcacccg gcgaaaggat gaaaaccaga atggcatcac ccggaataaa cttgtagttg
      781 aacagcgatc cgtttcccag catttcccta attgagccca acagcgtaag ggccatggtg
      841 aatccaattc ccattcccaa cccatccatc gctgaatcga gcacgctatt ctttcgggca
      901 aatgcctcag cacgacccag aacaatgcag ttaaccacaa ttagaggtat aaaaattccc
      961 agggtttcat agagcgactg cgtgtaggct tgcataagca ggtcaactat agtaacaaat
     1021 gtggcaatta ccacaatgta ggagggaatt cttaccttgt cggggataaa acgtgcaatc
     1081 atggagatta caacatttga tcccaacagc acaaaggtgg tggccagccc catgcctagc
     1141 ccgttaatag cagaagtggt ggtggccagg gtgggacaca tccccagcac tattacaaag
     1201 gttgggtttt ccctgataat acccctcgta aggttgctcc atgcgctcat tttctacccc
     1261 cttccttaaa agctttgtag gcgcggttaa ctgcatcgca aaaggctctg gacgagatgg
     1321 ttgaagcagt tatggcatca acgtcgcccc catccttctt caccataacc ttgaaagttt
     1381 ccggattttt accctcgaat tgaacggaaa aatcggactt tcctgggtct atcttatcac
     1441 ccaaaccggg agtttccttg tgagaaatta cagtaatctt gttgatggtt ccatcgggta
     1501 aaaagccgac catcagctca attcttccac caaagccatt gttgctgaat gttttaaccg
     1561 ctacgcccac aagctggcca ttcttggagg cgggatagaa aaacaaatca tcaccatcgg
     1621 cctttacagt gtatgcttcg ttggatggtt ggttgtcaaa ttcgggaaca accgccttaa
     1681 tagcgttgtt caccttagcc acctgagcgg ctgcaatagg atccttggtt gatttataaa
     1741 ccagccccaa tccggcagat gcaacaaggg taatcgccag cagggaaatg gtcatattct
     1801 taaatgatga ttctacaccg gacatatttt gatgtttaag ttttacatgc tatttaccgc
     1861 ttattgctgc tgcaccaaaa cgttttggct ttacgaatcg gttgataagg ggtacgaaag
     1921 cgttcataat cagaatggca aacgaaactc cctcagggta cgagccccaa acccttatta
     1981 tcacagtaag cgcgccaata cctattccgt atatccacat gcctttggga tgcatgggtg
     2041 aggtaacata gtcggtggcc atgaatatag cccccagcat cacaccaccg gtaaggaggt
     2101 ggaaaaccgg gtcggcatag ataccggggt tgataagcca taaaattcct gtgaatatga
     2161 aaatggtagt aaaaattgat accggaatat gccaggtaat tacgcgggta atgagaagcc
     2221 atgcaaaccc aataagcaag gcgaaagcag caatctcccc caacgagcca cccatcaagc
     2281 ccatgaaaag ctgcatgtgg gaaggaatgc tgtcggccat ttgcgaaatg gtttggccat
     2341 tcttaacccc ctccttaata atagccaaag gggttggacc ggtaatagca tcaatatact
     2401 gtaaacgaga ggtaaccggt agcggccagc tggtcatttg caccggaaag gatatcagca
     2461 ggaaaattcg tgcggctatg gccgggttga aaatattatt gcccagccct ccgaagctca
     2521 tcttggcaac gccaattgca aagaggcttc ccagcactat aatgtaccac ggcagattgc
     2581 tcggaacgtt aaatgccagc agcaaacccg ttacaaccgc agagcaatcg gatattgtcg
     2641 gcttaacctt gaaaataaac ttctgaatca ggtactcgaa tagcacgcac gaaagaaccg
     2701 atattagagt aacaattatg gctcccacgc caaagtagta aatggaaacg gcaaacgccg
     2761 gcatcagcgc taaaaccaca ttcagcatca gctggctggt cgatttgccg ctatgcacat
     2821 ggggcgatgg tgaaactaca aaacgattat ccattcgttt aaaaggttac tgtcagtata
     2881 tactttaaat gttaattttt ccgctcccta acaattttgc ctacctcaac cttgcctagc
     2941 ctgataaagt cgagcaaaaa caagttagcc ggacactcaa agctgcaaga accgcactcc
     3001 atgcaatcga gcacacgctc tacctccatt tggctatact gcattcgctg ccccagccgg
     3061 taaagcaagt agggctccaa ccccataggg caaacagaaa cgcacttggc acacctaatg
     3121 caaacacctt cgggtttacg ttttgattcg gcagcaggaa ttaccaatat tcccgaagtt
     3181 cctttcgtaa cgggcacatc aatggagcta actgcctttc ccatcatggg gccaccgctc
     3241 accaccttac ctgtatcatc tggtaggcca cctgcagcgg cgatcagtgc cgaaactggc
     3301 gttccaattc ttaccaaaaa gtttgatgga tgggcaagat ttttacccgt aacggtaaca
     3361 atgcgctcaa ccagcggctt atttttctgc accgcctcgt aaactgcaag ggctgtgccc
     3421 acgttatgaa caactgcacc tatatcaatt ggcaatccac ccgaaggaac ctcacggccg
     3481 gtaagcgcct taataagctg tttttcgcca ccctgagggt acttcacctt tagaggttga
     3541 acctctattc cgctgtatga cttggccagt gattggagat gggatacagc atcgggctta
     3601 ttcgcctcaa taccaaccat agccttaggc acattcagag ctttcatcaa aatctgaatg
     3661 ccaatcataa gctcgtggcc tttctcgagc atcagcctat gatcagaggt aagaaagggt
     3721 tcacactcaa caccattaat caccagcatt tcagccttct tgcctgcggg aacggaaagc
     3781 tttacatggg ttggaaatgt tgcaccgcca aggcccacaa taccggcctc cgctattttt
     3841 tttataatat cctgagccga aagggttatt tccttcttta agttgggaga acggtcaact
     3901 gaaggctccc aatcatctcc ctcaacgttg atggttatgg caggccgttt atagcctgaa
     3961 ctatccatca cgggttcaag ggactctact attcctgaaa cagttgaatg gacattggcc
     4021 gacacaaagc cagcgctttt ggctatgagt tgtccggttt ttaccctatc gcctttggct
     4081 accatcgcct ctgccggggc acctatatgc tgcgacagag gaatgatgac tttagccgga
     4141 agaggcaaaa cctcaaccgg ttgagctgct gaaatcttat tttcaggtgg atgaactccc
     4201 ccctttttaa atgttttcaa cacgtttagc aatttttttt aaaattaagt ttgatatggc
     4261 ttatgctagc tggttactgc gttctgggct tgttcctcaa ccttcggctt acgtggaggg
     4321 aagtttacct ccaaaatgga atcggttggg cattcttcca cgcacttcct gcaaagcttg
     4381 cacttgttgg catcaatgta ggccaggtta ttctccaggg tgatggcctc gtaagggcaa
     4441 accttcacgc acttgaaaca cccaatgcat gcaacgctgc agtacttacg tgcaacacca
     4501 cccttgtcgg tattcatgca cgacacgaaa attcgcttgc cctttggccc ctttttccgc
     4561 agctctataa gtaattttgg acatgccttt acgcatgccc cacaagcagt gcacttctcc
     4621 tcgtctataa ccggaagaag ggaaacgggg tcaatataga tggcatcaaa cttgcaaacc
     4681 gcaacacagt cgccaagacc taagcatccg aattggcaac ccgtatcgcc gctgtaaagt
     4741 gtggatgcaa atttgcaaga agatattccg ttatactcta caactcgcgt cctgttttgg
     4801 gggcttcccg aacaacgcac tacggcaatt tgcggatcct tgtcctcaac tgctttacca
     4861 agtatggcag ccacctttct catggtttcg ttaccgccaa cagggcaaaa gatcgcgctg
     4921 atatcgtctg ccttaacaag cgcatcggcc aaccccctgc acccgggata accacatccg
     4981 ccgcagttgg cacctggcag cagggcttca acctcatc
//
LOCUS       DAOPHX010000156         4982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_22544_length_4982_cov_7.593262, whole genome shotgun sequence.
ACCESSION   DAOPHX010000156 DAOPHX010000000
VERSION     DAOPHX010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22544_length_4982_cov_7.593262"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PK502_09690"
     CDS             complement(<1..619)
                     /locus_tag="PK502_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPH01130.1"
                     /translation="MSTTIHPESLQGLSKAEAARRLIEEGYNELPSSKPKNVFQIALG
                     VVKEPMFILLVACGTLYLILGDVNEGLMLLSFVFVIMGIEFYQEKKTERALDALKNLA
                     SPRALVIRDGETVRIAGREVVTGDYVVLQEGDRVPADGVILSSINLSADESLLTGESV
                     PVRKREWLEGDGSFIPGGDDIPMVFSGSMVVNGNGVFRVTDTGVNT"
     gene            complement(804..1217)
                     /locus_tag="PK502_09695"
     CDS             complement(804..1217)
                     /locus_tag="PK502_09695"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPH01131.1"
                     /translation="MESMEKVIAGLSNMIGDAEEKAKEQFNTLNLTITQMHYLETINL
                     LGNPNITELSAQLKLSKPTVTVSVDKLIEKGCVYKIQSDEDRRSAHIHLTEKGKLVNQ
                     MHDYAHRKIAEMVKNRLSAEELTLLERLLSKVLVE"
     gene            1520..2023
                     /locus_tag="PK502_09700"
     CDS             1520..2023
                     /locus_tag="PK502_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01132.1"
                     /translation="MNNAFATPQDNHERLLRMVEEIFATSGDPRQLNITPEVIDRLKQ
                     LHPSTVLEHVSENGTVAWVVLIPTTLELMNRFVAGEISEKELFELTPLGIKYEALYLC
                     SALVLEEYRRQGIVKQMVTAAAQSIRKEHPLKAIFVWPFTKEGELAAESIATTLNLPL
                     YKFQHDK"
     gene            complement(2024..3382)
                     /locus_tag="PK502_09705"
     CDS             complement(2024..3382)
                     /locus_tag="PK502_09705"
                     /inference="COORDINATES: protein motif:HMM:NF017396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HPH01133.1"
                     /translation="MKRMPQQQRPLRIRGLILSIFFPVLMVVILSGNPKPITPTEVLS
                     SIALVDSFRAIPSDNPFSETYEVWFRVPLDHTNPHSQYFPLRVYYSHNGFNKPMVVVI
                     DGYTMYTSKANEITSIVDGNQITIEHRFFSQSRPLDSIPWSYLNIRQAAADAHLVISA
                     FKPFYHGKWISTGISKSGQATIFHRRFYPADVNVSIPYVAPLNFSSEDERVYKFLDSV
                     GTPECRKKIYDFQIQLFERKSEIMPLFKELSASKGWKFTMGFEKAYDLSVLEYSFAFW
                     QWGFNCSSIPASSSTAKELFEHWLEVNPFSFFEENEVDKVRPFFYQAMTEIGMYGYRV
                     KPFSRYLSDTANITFSFAMPRGHRAIFNASAMSDIDRWVKDSGSYMLYIYGQNDAWSA
                     TAVDPGRKTNAVKMVCPGGSHSSRIKDFPPELQDSIYKVLGRWLDLKLPANGYTNSSS
                     GL"
     gene            complement(3384..4943)
                     /locus_tag="PK502_09710"
     CDS             complement(3384..4943)
                     /locus_tag="PK502_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HPH01134.1"
                     /translation="MPDKSIKTYGIFKISVLKTFQICPSVNPEELALYLPIRYNEPMD
                     ASSNSYLSRINSWCKRGKSFDLAVITHYLQNYTQNISAGYSGEAVRIFDNYSMHNFIE
                     HGEPDNGSDLLAILLDEGVKVPFPSPKQPSFTFIDLFAGIGGFRLAMQELNGKCVFSS
                     EWNTESQATYMVNFGEFPFGDITLQEVKDLIPYGFDLLCAGFPCQPFSKGGHRQGFDD
                     TRGTLFFDICQIVMKHRPKFLLLENVANLAGHDGGNTYSVITRSLDELGYYFPRNPMI
                     LSPDKFGVPILRPRLYIPCVRKDLAAGKEDFIWNFEEEIWKHPSENQLTVNDIVDPNV
                     DDCISPYEEKVLTAWDEFYRGIDQKVIGFPVWMDFFRFEGDLSQFPVWKATFIRKNID
                     LYRRNRSFIDKWLAKWDNLEWCSKTHRKFEWQAGDGYSSLFDCLIQFRPSGIRVKRPD
                     KFSTLVAMNHRQIIGRFRRRISVEETKLLQSFPKEYNLIGPPATALRQLGNSVNVRVV
                     KNIFGLVIKNF"
BASE COUNT         1402 a         1156 c         1023 g         1401 t
ORIGIN      
        1 cggtattgac cccggtgtcg gttacccgaa acactccatt gccgttcacc accatggagc
       61 ccgaaaaaac cattggaata tcgtcccctc ccggaataaa agagccatct ccttcgagcc
      121 attccctttt tctcaccgga acagactctc cggttaacaa cgactcatct gctgaaagat
      181 taatggagct gagaataacc ccatcggccg gaaccctatc gccctcctgc aaaacaacgt
      241 aatcaccggt tacaacttcc cgtccggcaa tccttactgt ttcgccatct cttatcacca
      301 acgcacgtgg actagccagg ttttttaatg catccagcgc cctttcggtt tttttctcct
      361 ggtagaattc aattcccatg ataacaaaca caaagctgag gagcataagg ccttcattaa
      421 catctcctaa aataagatac aaagtgccac aggcaaccag aaggatgaac attggctcct
      481 ttaccacccc aagggctatc tgaaaaacat tcttgggttt tgaggagggc agctcgttgt
      541 acccttcttc tataagtctt ctggcggctt ccgcttttga aaggccttgc aggctttcag
      601 gatgaatagt tgtgctcatt ccttgtcaac ttaagtcaac gtgtatataa aaagtccatg
      661 cttatccgtg ttgttcgctt gaacaacgct taacttgtta gattaatcta actaatcggg
      721 taaaagtgtc aagcatatct attaatattt cataccaaat tctggcaata aaacaaaaga
      781 gtcctgtttt caaaacatgg ccgttactct accagcactt ttgaaagtag gcgttcaagc
      841 agggttagct cctccgccga taagcggttt ttaaccattt cggcaatttt tcggtgtgca
      901 taatcgtgca tctggtttac cagcttcccc ttctcggtga ggtggatgtg cgcgcttcgc
      961 ctatcctcgt cggactgaat tttataaaca catcccttct caattaactt gtccaccgat
     1021 acggttacgg tgggcttcga gagcttcagc tgggccgata gttcagtgat gttggggttt
     1081 cctaacaggt taattgtttc caggtagtgc atctgggtaa tggtcaggtt gagcgtgttg
     1141 aactgctcct tggccttctc ctccgcatcg ccaatcatgt tggacaaacc cgcaataact
     1201 ttctccatgc tttccataca gcaaatatag ttagtttgta caaactaaaa ttaatgctaa
     1261 ataaaatttc ggttaagcgt aggcataaaa tgataatttt ttgagcaaaa aaatcagctg
     1321 aactttcaca ttccattttt tacactactc tgcacacccc aaatataatg aaatctacct
     1381 taagtgctta atttctggct gttacgtttt caagggttgc catatattac cataataaca
     1441 aaaagttagg ataccgtcac agctgaatat acgctcgaaa gctatatttg caatgtttaa
     1501 taaccggatt tcaaaccaca tgaataacgc ttttgcaacc ccgcaggata accatgaaag
     1561 gttgttacga atggttgagg aaatatttgc cacttctggc gatccccgcc agctgaatat
     1621 tacccccgag gtaattgacc ggctgaaaca gcttcatccc tctacggttt tggagcatgt
     1681 tagcgagaat ggaaccgtgg cctgggttgt gctgattccc accacgctgg agcttatgaa
     1741 ccgatttgtg gcaggcgaaa tttcggaaaa ggagcttttt gagctaacgc cattaggcat
     1801 aaagtacgag gcgctttacc tctgctctgc actggtgctg gaggagtata ggcgacaagg
     1861 aattgtgaag caaatggtta ctgctgctgc ccaaagcatt cgaaaggaac atcccttaaa
     1921 agccatcttt gtttggccat ttaccaaaga aggagagctg gcagcagaaa gcattgctac
     1981 tacactgaat ttgcccctgt acaaatttca acatgataaa tgattacaac ccggagctag
     2041 aattagtgta gccattggcg ggtaacttta ggtcgagcca cctacccagc accttgtaaa
     2101 tgctatcctg aagttcgggc ggaaaatctt taatccttga cgagtgcgaa cctccggggc
     2161 ataccatttt tactgcattg gtttttctac ccggatcaac agcagtagcc gaccatgcat
     2221 cgttttgccc gtatatgtat agcatgtagc tacctgaatc ctttacccaa cggtcaatgt
     2281 cgctcatggc ggaggcgttg aagatcgccc ggtgccctct tggcatggca aagctaaagg
     2341 ttatgttggc ggtatcgcta aggtagcggc tgaacggctt caccctgtag ccatacatgc
     2401 ctatctcggt catagcctga tagaaaaatg ggcgaacctt gtcaacctca ttctcctcaa
     2461 aaaaggagaa ggggttaacc tccagccaat gctcaaaaag ttcctttgcg gttgaactgc
     2521 ttgcaggaat tgacgagcaa ttaaatcccc actgccagaa agcaaaggag tactcaagca
     2581 cgctaaggtc gtacgccttt tcgaatccca tagtgaattt ccaccccttg cttgctgaaa
     2641 gttccttgaa gagtggcata atttcacttt tcctttcgaa aagttgaatc tgaaaatcat
     2701 atattttttt tcggcattcg ggcgtcccaa cactatcaag aaatttgtac accctttcgt
     2761 cttcacttga aaagttcagc ggagccacgt agggtatgct aacatttaca tcagcgggat
     2821 agaagcgccg gtggaaaatt gtagcttgtc cactcttgct tatcccggtt gaaatccact
     2881 tcccatggta gaacggttta aatgctgata ttaccaaatg tgcatcagct gccgcttgcc
     2941 taatattgag gtaactccag ggaatggagt ccaatggtct gctttgagaa aaaaagcgat
     3001 gctctatggt tatctgattc ccatcaacaa tactggttat ctcgttagct ttcgaagtgt
     3061 acattgtgta gccatcaata acaacaacca tgggtttgtt aaaaccatta tgggagtagt
     3121 aaacccttaa gggaaaatat tggctgtgag ggttggtgtg gtcaagcggt accctaaacc
     3181 aaacctcgta ggtttccgaa aaggggttgt cagaaggaat tgctctgaag ctatcaacca
     3241 gcgctatgga cgacagcact tctgtaggag ttatgggctt ggggttgccg gaaagtataa
     3301 ccaccatcaa aaccgggaag aatatactca aaataaggcc tcgtatcctc aggggtcttt
     3361 gttgctgcgg cattcgtttc atgctaaaaa tttttaatta ccaatccgaa aatgttcttt
     3421 acaaccctaa cgtttacgga attacctagt tgcctaagcg cagtagccgg cggtccaata
     3481 aggttgtatt ccttgggaaa agattgaaga agcttggttt cctcaacaga aattcttcgt
     3541 ctgaacctgc caataatctg ccggtggttc atggcaacca gggttgaaaa cttatcggga
     3601 cgcttaaccc taatccccga cggacgaaat tgtatgaggc aatcaaacag gctgctataa
     3661 ccatctccag cctgccactc gaacttgcga tgagtttttg aacaccattc caggttatcc
     3721 cattttgcca gccatttatc aataaaacta cgatttcgtc tatacaagtc aatattcttt
     3781 ctaataaatg tggccttcca tactgggaat tgggataaat cgccttcaaa tctaaaaaag
     3841 tccatccaca cgggaaaccc aatcactttc tggtcaattc ccctgtaaaa ctcatcccaa
     3901 gcagtaagaa ctttttcttc gtatggagaa atacaatcat caacgtttgg atcaacaata
     3961 tcgttcaccg ttaactggtt ttcagacgga tgcttccaga tttcttcttc aaaattccaa
     4021 ataaaatctt ccttccctgc tgccaagtcc ttcctaacac agggaatata taatcgtgga
     4081 cgaagaatgg gaactccaaa tttgtctgga gagagaatca tcgggttgcg tggaaaataa
     4141 taccctaact catcgaggct tcgggtaata acgctatagg tattcccgcc atcgtgcccc
     4201 gcaaggttgg ccacattctc gagtaataaa aattttggcc tatgcttcat tacaatttgg
     4261 cagatgtcaa aaaaaagcgt tccacgtgta tcgtcaaatc cttgcctgtg tccccctttt
     4321 gaaaaaggct ggcagggaaa acccgcacaa agcaaatcaa aaccgtaagg tattagatcc
     4381 ttaacctcct gtagagtaat atcgccaaaa ggaaactctc caaagttgac catataggtt
     4441 gcttgcgatt cggtgttcca ctcgctgcta aacacgcatt taccattaag ctcctgcatg
     4501 gccaaacgga aaccaccaat tcctgcaaat aaatcaataa atgtgaatga tggttgcttg
     4561 ggcgagggaa agggaacttt aaccccctca tctaaaagga tcgctagcaa atcgcttcca
     4621 ttgtcaggtt ctccatgttc aataaaatta tgcatgctgt agttatcaaa aatccttacc
     4681 gcctctccac tatagcctgc tgaaatgttt tgggtataat tttgcaggta gtgagtgatg
     4741 actgcgaggt caaatgattt tcctctctta caccatgaat taattcgact taaatagcta
     4801 ttactagagg cgtccattgg ttcattgtat cgaataggca aatatagagc caattcttca
     4861 ggattaacgc ttgggcatat ttgaaatgtt tttagaaccg atattttgaa tattccatag
     4921 gttttaatac ttttgtccgg catattgaag gagggataac aaccatctat cttataaagt
     4981 tt
//
LOCUS       DAOPHX010000157         4981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_22551_length_4981_cov_6.248071, whole genome shotgun sequence.
ACCESSION   DAOPHX010000157 DAOPHX010000000
VERSION     DAOPHX010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22551_length_4981_cov_6.248071"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..4767)
                     /locus_tag="PK502_09715"
     CDS             complement(160..4767)
                     /locus_tag="PK502_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01135.1"
                     /translation="MSRRPARKPRIAATLGLILLALLSLRINALHQDEGKFSGNPLLF
                     PTEVKTIIPDLSKDDEAYLKAFSIFWLSDSIPEDQKGNIIETANLILPILPESKAIVL
                     DYIKLAATFYRDSYANKNYAPWNGALKQLLLTENLDYNKLLAFFRFTRNTVDSSFLRQ
                     TISYTWKVSPHHSYNFEFSSNQLLARFTNVNLVCITGTDSIYIEGTSGYYNPENDMWV
                     GSKGKVTWVRSGYPKDAIFAMLSSYRFPLSKNSYTVDSVYFTNKDYFDRPARGTLTDK
                     LVKDYKPTTIDYPEFKSYDNWFKIKNLFERVDYEGGYIMRGSRLLGVGSGEQLAKVSI
                     SRNGKPFLKVEGNIMIFERSILTADRASILFRFEEDSIYHNGLYFSYNNNTRTVTILP
                     TERITSQSPFYSSYHKLSIWTDQLSWNIDGDKLIFGASRGSSIGNASFESNNFFNEVY
                     FDQLMGPSDRHPLLEVWNFSVRNKSKTFLATDLAQKLRKPVEEVKIEMMRMAKLGYVL
                     YDFETDEVQVTEKLKHTVMAKFQKVDYDVIRFSSTCPQSQPNAELDIASMNMKINGVN
                     NISVSDSQNVFINPYNQQVIMQKNRNFEFAGEVQAGLFRFYGSKYRFNYDEFKIDLLQ
                     VDSLKLDYQTRYRDGYGRKILQSVANTFHIIGGDIVIDKPSNKSGLQSNPDYPIFHST
                     QKSYVYYDDPSIFNGIYKRDSFYFEIQPFTYVNMDNFEQSDLDFKGILYSRNILAPIE
                     ENLVLRPDNSLGFIKSTPPEGLALYRGKGKVFDKIDLSNRGLTVKGQVGYITSTTRSD
                     DIFLFPDSMVTSAASFTMEKQVAGVEFPNVTGDLHHVKWLPQNDKLFVTKGEKPFKMY
                     DSLGIFSGDLLLQPLGLTGIGSMSIPNAALSASLFEFNADDFTTPVSSLTLYSPANQN
                     VALTTKDVRSKISFKSQTGEFSKTNQSLFATLDELGYEAYLDRFSWNMVTNNLAMQTL
                     MQQQDVKDGKFRVSNMVEGDSIPKGSLFYSVKSSEDSLYFFSAKSTYNTATAELNADS
                     VSYIVVADATIMPKDKKIRVNKPNRMLPLSEATIKANNLTAYHRFFDADVSIISRNSY
                     SGKGHYYYADENDSLQTIYFTQIKVDSARNTVATGTITEPDSFTLSPQFAFVGQVHLS
                     APQKLLYFEGGAKPSYQCTGTTGQWIKFEALIDPRRVLIPITEQPKNLNLHPLISGSV
                     ISEDSIKVYGGLLRNRKNYDDKPLVNASGYLSFNNNNRRFTIAPLHKLSNPDTSGSSV
                     SLQKDFCMVFSDGLVKLPVNLGQVKLNAAGSTTHKLEGNDLTLDLVLQLNFHFNQKSL
                     EAIALELNQSFALEKVDLSRKLYRKALYQLVDSSELHAAISQLDLFGSFSQIPKGYES
                     TITFSDLKMRWNPLTKSFVSKGKLGIGTIGNIQVNRFVNGYIEIFKRRSGDLMTLYLD
                     FGEDRYYVFTYTKSVMQVSSNNPEFVQPIEQQKSSETRLKVKPGEPGYRYLIGTKKAL
                     DQAQQRYNQLMSGDEDKVAPPDDEVVEEGRDKVKEKGEKRN"
BASE COUNT         1209 a         1092 c         1223 g         1457 t
ORIGIN      
        1 gtaaaaaatt gttcgccccg tacaaggggc tgtgcccctt gctacttatt ccgcctcttc
       61 ggggcttaaa ccaccagaca agttctgaat ttggaatccg ccagtaggcg gacaagtttc
      121 gtcaggagcc ggacaagctc cgccggtagg cggataagtt taatttctct tttctccttt
      181 ctcctttacc ttatcccttc cttcctcaac tacttcatcg tcgggaggtg ccaccttgtc
      241 ctcatcaccg gacatgagct ggttgtaacg ctgttgggcc tggtccagag ctttttttgt
      301 acctatgagg taacggtacc ctggctcgcc gggtttaacc tttagcctgg tctcgctcga
      361 tttttgctgt tcaatgggct gaacaaactc gggattgttc gatgaaacct gcatcacgct
      421 ttttgtatag gtaaaaacgt agtacctatc ttcgccaaag tccaggtaaa gggtcatcag
      481 gtcgcccgac cggcgtttaa agatttcgat gtaaccattc acaaaacggt taacctgaat
      541 attgccaatt gtgccgatgc ccaactttcc cttggaaaca aacgatttgg ttaaggggtt
      601 ccatctcatc tttaagtctg aaaaggtgat tgtactttcg tatcccttgg gaatctggct
      661 aaaacttcca aataaatcca gctggctgat ggcagcatgc agctcggatg aatcaactag
      721 ctggtaaagc gcttttcggt agagttttct tgaaagatca accttttcga gcgcaaacga
      781 ctggtttagc tctagggcaa tagcctcaag tgatttctga ttaaagtgga agtttagctg
      841 tagcacaagg tcaagggtta gatcgtttcc ttctagctta tgagtagtgc tgcctgcagc
      901 attaagctta acctgaccga ggttgacagg tagcttaacc aggccatcgc taaaaaccat
      961 gcagaaatcc ttttgtaggc taacgctact acccgatgta tcgggattgc ttagcttgtg
     1021 tagcggagca atggtaaatc tacggttatt gttgttaaag ctcaggtatc ccgaagcatt
     1081 aaccagcggt ttgtcatcgt agtttttcct gttacgcaga agtccgccat atacttttat
     1141 cgaatcctcc gaaattaccg acccgctgat taacggatgg aggttgaggt ttttgggctg
     1201 ttcggttatg gggataagaa ctctcctggg atcaatcaac gcttcaaatt ttatccactg
     1261 gcctgttgtt cccgtgcatt ggtatgatgg tttggctcct ccttcgaaat agagtagctt
     1321 ttggggcgcg gagaggtgta cctgtccaac aaaggcaaac tgtgggctaa gcgtgaagct
     1381 gtctggttcg gttatagttc cggtagcaac tgtgttccta gcgctatcca cttttatctg
     1441 ggtaaagtaa atagtttgaa gcgaatcgtt ttcgtcagcg tagtagtagt gaccttttcc
     1501 gctatagctg tttcggctga taatagatac atcggcatcg aagaaccggt ggtaggccgt
     1561 aaggttattg gccttgatgg tggcttcgga aaggggaagc atgcggttcg gcttgttcac
     1621 cctgattttt ttatcctttg gcataatggt ggcatcggct accacgatat agctaacact
     1681 atcggcgtta agttcagctg ttgctgtatt atacgttgat tttgccgaaa aaaagtaaag
     1741 cgagtcttcg ctgcttttta ctgagtagaa gagagacccc tttgggatgg aatcaccttc
     1801 aaccatgttg gaaaccctaa atttaccgtc cttcacatcc tgctgttgca tcagggtttg
     1861 catagccagg ttgttggtaa ccatattcca gctaaaacgg tcaaggtagg cctcgtatcc
     1921 caactcatca agcgtggcaa aaaggctttg gtttgttttg gagaactctc ctgtttgact
     1981 tttaaagctg attttcgacc ttacatcctt tgtagtaagc gcaacattct ggttagctgg
     2041 tgagtacagg gttaggctcg ataccggcgt ggtaaaatcg tcggcattga actcaaatag
     2101 gctggccgaa agagctgcgt ttggaatgct catacttcct attccagtaa ggcctaatgg
     2161 ttggagtagc aagtcgccgc taaatatgcc cagggaatcg tacattttaa agggtttttc
     2221 cccctttgta acgaaaagct tatcattttg gggtagccat tttacatggt gaaggtcgcc
     2281 tgtaacattt gggaactcaa ctccagcaac ctgcttttcc attgtgaatg acgcagcact
     2341 ggtaaccatg gaatcgggga aaaggaaaat atcgtccgac cgggtggtag aggtaatgta
     2401 gccaacctgt ccttttacag taagccccct gttgctcagg tcaattttat caaaaacttt
     2461 cccttttcca cggtagagcg ctaaaccctc gggtggagtt gacttaatga aacccagcga
     2521 gttatcgggt cgaagcacca ggttttcctc aatgggggca aggatgttgc gggaatacag
     2581 aataccctta aagtccaggt cagactgctc aaaattatcc atattcacgt aggtgaaggg
     2641 ttgtatttcg aagtagaacg aatcacgctt gtatatgccg ttaaagattg aggggtcgtc
     2701 atagtaaacg tatgattttt gagtactatg aaaaatgggg tagtcaggat tactctggag
     2761 tccggacttg ttgctgggct tatcaattac aatgtcgcca ccaatgatgt gaaaggtgtt
     2821 ggctacgctt tgcaggattt tacggccata gccatctcgg taacgggttt ggtagtcaag
     2881 tttcagcgag tcaacctgta ggaggtcgat cttaaactcg tcgtagttga agcggtattt
     2941 cgagccgtag aacctgaata agccggcttg cacttctccg gcgaactcaa agttacggtt
     3001 tttctgcatg atgacttgct ggttgtatgg gttgatgaat acgttttgcg aatcgctcac
     3061 cgaaatgtta tttacgccgt taatcttcat gttcatactg gcaatatcaa gctcggcgtt
     3121 gggttggctt tgaggacagg ttgatgagaa gcgaatcacg tcgtaatcaa ccttttgaaa
     3181 cttcgccatc accgtatgct ttagtttttc ggtaacctga acctcatcgg tttcaaaatc
     3241 gtagagcacg taccccagct tggccattcg catcatttca attttaacct cctccaccgg
     3301 cttgcgcagc ttttgggcca gatcggtagc cagaaaggtt ttgctcttgt tcctgacgga
     3361 aaagttccaa acctccagta gagggtgacg gtcgctgggg cccataagct ggtcgaagta
     3421 aacttcattg aaaaaattat tggattcaaa gctggcattc cctatgcttg atcccctcga
     3481 ggcgccaaaa atcagcttat cgccatcaat attccatgaa agttggtcgg tccaaatgct
     3541 cagcttgtgg taagagctat agaaagggct ttgcgaggtg atgcgttcgg tgggtaaaat
     3601 ggttaccgtt cgggtgttgt tgttgtagct gaagtaaagg ccgttatggt aaattgagtc
     3661 ctcctcgaac ctgaaaagaa tgctggctct gtcggcggtg aggattgacc gttcaaaaat
     3721 cataatattc ccttccactt tcaggaacgg tttgccgttt cggctgattg atacctttgc
     3781 cagctgctca ccagaaccca cgccaagcag ccgcgacccc ctcatgatgt aacctccttc
     3841 gtagtcaacc cgctcgaaca ggtttttaat tttaaaccag ttatcgtacg atttaaactc
     3901 ggggtaatca attgtggtcg gtttataatc ctttaccagc ttgtcggtga gggtgccgcg
     3961 tgccggacgg tcgaagtagt ccttgttggt gaagtaaacc gagtccaccg tgtaggaatt
     4021 tttggaaagg ggaaaccggt agctcgacag catcgcaaaa atggcatcct tcgggtagcc
     4081 cgagcgtacc caggtaactt tacccttact tccaacccac atgtcgtttt cgggattgta
     4141 gtaaccactg gttccttcaa tgtatatgct atcggtacct gtaatgcata ctaggttgac
     4201 gttggtaaac ctggctagca gctggttact gctaaattca aaattgtagg aatgatgggg
     4261 acttaccttc cacgtgtagc ttatggtttg ccttaagaac gagctgtcaa cagtgtttcg
     4321 ggtaaacctg aagaaagcca gcaacttgtt gtagtcaagg ttttcggtaa gcagcaactg
     4381 ttttagcgcg ccattccagg gtgcatagtt tttgttagca tagctatccc tatagaatgt
     4441 agcagccaac ttgatgtaat ccagaacgat agccttgctc tcgggtagaa tgggtagtat
     4501 taggttggcc gtttcgataa tatttccctt ctggtcttcg ggaatggaat cggaaagcca
     4561 gaaaatgctg aaagccttca ggtaggcctc gtcatcctta ctcaaatcgg gaattatggt
     4621 ttttacctcg gtggggaaga ggagcgggtt gcccgagaat ttcccttcgt cctgatgcag
     4681 ggcgtttatt cttagcgaca gcagcgccag caatatcaac ccaagtgtag ctgctattcg
     4741 aggctttctg gctggtctgc ggctcatgac ggaggatgag attaagttag tgctgccatt
     4801 cagcttccga atggtaataa acctatttta caggctgcta aaggtttaac tccctgctct
     4861 ttggacacat tactcaacac ggtttgggac attttccctt tcatcctgtt taagcaaagg
     4921 tagcgcaagt tctgaatttg tcaagggtag tacggacgat gacttgttgg ttatttctta
     4981 a
//
LOCUS       DAOPHX010000158         4951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_22749_length_4951_cov_6.102737, whole genome shotgun sequence.
ACCESSION   DAOPHX010000158 DAOPHX010000000
VERSION     DAOPHX010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22749_length_4951_cov_6.102737"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..2313
                     /gene="ppk1"
                     /locus_tag="PK502_09720"
     CDS             214..2313
                     /gene="ppk1"
                     /locus_tag="PK502_09720"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPH01136.1"
                     /translation="MGRKNLPLVNREISWLSFNGRVLQEAEDKNVPLIERLKFLGIFS
                     NNLDEFFRIRVATLRRLLVVEKGAKKVMGDNPRKILEKIQRLVVSYQEKFDSIYSDVK
                     LQLRAEGIYIMDETELDPDQVDFVRSLFKRKISMSLGPIMISRLDRFPQLVDQSIYLA
                     VKLSSSENRQAKEYSLIEIPTRNISRFIRLPRKDNQEYIILLDDVIRLCLDMVYSILP
                     YDRFEAYTIKITRDAELDVDNDISESLLDKISKGVKNRKRGEPVRLVYDEKIAPDLLN
                     FIVKGLSLDEKDYILPGGRYHNFKDFMDFPNFGRSDLVNAPMKPVQVAELENASSILG
                     VMAKHDFILHYPFHDFAYYVKMLREAAIDPKVTAIYITLYRMAAESRVVNALMNAAQN
                     GKKVTAVIELRARFDEHANIYWAKQMQEAGVNVVFGMPGIKVHSKMTLIIRHEGRKDV
                     RYAVVSTGNFHEGNANLYTDVNLFTVNQGIAREVEKVFTFLEFNYKTFSYKHLLVSPV
                     NMRRKLYSLIENEMDNAAKKLPAYIHCKINNLVDTEMIAKLYQASQAGVEIKLVVRGI
                     CSLVPGVKGVSDNIEVFSIVDRLLEHSRIFIFANSGKETCYISSADWMTRNLDYRVEI
                     ATPIYDKHLISELKTVINMALKDNVKSRVIDQKQSNSFRNPEAGEEPFRSQLELYRYY
                     KSKENLQSAATEDYNLT"
     gene            2413..3306
                     /locus_tag="PK502_09725"
     CDS             2413..3306
                     /locus_tag="PK502_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HPH01137.1"
                     /translation="MKLLKFAAIDIGSNAVRLLLSNVVENGDSVVFRKSSLIRMPIRL
                     GEDAFETGIISEARIQKLIKTMIAYRNLMEVEDVVAYRACATSAMREALNAPEIVERV
                     SRQTGVNIEVINGRQEAEIVYSNGIAETLDSSKPFIYVDVGGGSTEISLFQNGQNIAS
                     QSFDIGTIRMLKEKVGKSEFDLIKKWLKNLKITGGKPKIIGSGGNINKLYRLAGKVEG
                     RPLSFAELQKLFAQLKSYTFEERISILGMNPDRADVIIPAARIYLKTMEWTGASKILV
                     PTIGVSDGIVRMLYKNYRSRS"
     gene            3434..4741
                     /gene="mtaB"
                     /locus_tag="PK502_09730"
     CDS             3434..4741
                     /gene="mtaB"
                     /locus_tag="PK502_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPH01138.1"
                     /translation="MVEQRKRVAFYTLGCKLNFSETSTIARQLVEMGYERVSPDDKAD
                     VYVINTCSVTEHADKKCRQAIRKFASRSPGALIAVVGCYAQLKPDEVAAIEGVDLVVG
                     ASAKFQLPQLLATHVGKDGAKVYSCDIDRVADFSPAYSSGDRTRSFLKVQDGCDYHCS
                     YCTIPLARGKSRNMPVTNIVAQAQAIAAQNVKEIVLTGVNTGDFGRSTGESFIELLKE
                     LDAVEGIERYRISSIEPNLITDEIAHFVASSKKFLPHFHIPLQSGSNRILGLMRRRYN
                     RELFAEKIAMIRRLMPDTFFGVDVIVGFPGETEADFMDGYSFIREVNPAFLHVFPYSE
                     RPNTAAALLPAKVDAREIKVRADRLGALSSELHRQFYISRTGQVHHVLLEASQKSGLM
                     FGFTENYIRVAVPYNRNLAGKVVDVRLAALNPNGFMEGELMQP"
BASE COUNT         1350 a         1101 c         1179 g         1321 t
ORIGIN      
        1 ttccaacatc cggccttgtc gttcttcagt ttgatgcaga tagctggatg aatatttcga
       61 aatcgacact aaccgatagt ttcaccgtat ttccctgata cagggttaat ttgctacgat
      121 atcagcataa accttaacat ttagataaca tttcgtttac gcattgttcg aaaaatactt
      181 cttaaaattg aactttttaa cctaaactaa gctatgggcc gaaaaaactt gcccttggta
      241 aacagggaga ttagctggct ttcattcaac ggacgggtgc tccaggaggc agaggataaa
      301 aatgtgccat tgatagaaag attgaaattt ctaggcatct tctccaataa cctcgatgag
      361 ttttttcgaa tccgcgtggc cacgcttcgg cgtttgctgg tcgttgaaaa gggtgcaaaa
      421 aaagttatgg gtgataaccc cagaaaaata ctagaaaaga tacaacgcct ggtggtttcg
      481 tatcaggaaa agtttgattc tatctatagc gatgttaagc tgcagctgag ggctgagggt
      541 atttacatta tggatgagac ggagctcgac cccgatcagg ttgattttgt tcgctccctg
      601 tttaaacgaa aaatctccat gtcgcttggt ccaatcatga taagccggct tgaccgtttt
      661 ccccagctgg ttgatcagtc tatttacctt gctgttaagc tctctagtag cgaaaaccgt
      721 caggccaagg agtactccct tattgagatt cctacccgta acatttctag gttcattcgc
      781 ctaccccgca aggataacca ggagtacatt atcctgctcg acgacgtaat taggttgtgc
      841 ctggatatgg tttacagcat cctgccctat gataggtttg aagcctacac catcaaaatt
      901 acccgcgatg ctgagctgga tgttgacaat gacatatcgg agagcttgct ggataagatt
      961 tctaaaggtg ttaagaatag gaagcgtggc gaaccggtga ggctggttta cgacgaaaag
     1021 attgcccctg atttgctcaa tttcatcgtg aagggtttat cattggacga gaaggactac
     1081 attttgcccg gtggtcgcta ccacaacttt aaggacttta tggattttcc aaattttgga
     1141 cgctccgacc tcgtcaatgc ccctatgaaa ccagtgcagg ttgccgaact tgaaaatgcc
     1201 agcagcattc ttggagtaat ggccaagcac gactttatcc tgcactaccc cttccatgat
     1261 tttgcctact acgttaaaat gctccgtgag gctgctattg atcccaaggt aactgctatt
     1321 tatattaccc tataccgcat ggctgccgaa tcgcgggtgg ttaacgcttt gatgaacgct
     1381 gcccaaaatg gcaagaaggt taccgctgtt atcgagcttc gcgcccgttt tgacgaacat
     1441 gccaacattt actgggctaa gcaaatgcag gaggccggcg ttaacgtggt gtttggaatg
     1501 ccgggcatta aggttcactc taaaatgacc ctgattatcc ggcacgaagg tcgaaaggat
     1561 gttaggtatg ctgttgttag cacgggtaac tttcatgagg gtaacgccaa cctctacacc
     1621 gacgttaacc tttttactgt aaaccagggc atcgccaggg aggttgaaaa ggttttcacc
     1681 tttctggaat ttaactacaa gacatttagc tacaaacacc tgcttgtttc acctgtaaat
     1741 atgcgtcgga agctgtactc ccttatcgaa aacgagatgg ataatgctgc caaaaaacta
     1801 ccggcctaca tccactgcaa aatcaacaac ctggttgata ccgaaatgat tgccaagctc
     1861 taccaggcta gtcaggccgg ggttgaaatt aagctcgtgg ttagggggat ttgctccctt
     1921 gttccggggg taaaaggggt gagcgataat attgaggttt tcagcattgt tgacaggctg
     1981 ctggagcatt cccgaatttt tatttttgct aatagtggaa aggagacctg ctacatttca
     2041 tctgccgact ggatgacacg taatctcgac taccgggttg aaatagcaac acctatttac
     2101 gacaaacatt taatttccga gcttaaaacc gtaataaaca tggcgttaaa ggataatgtt
     2161 aagtcaaggg ttatcgatca gaaacaaagc aactccttta gaaaccctga ggcgggagaa
     2221 gaaccatttc gctcacagct tgaactttac aggtactata aatcaaaaga gaatttacaa
     2281 agtgctgcca cggaagatta caacttgacg taagagtata tcgctcccct ggcatgtact
     2341 actgtattga atttatgcat ttcttttatt cattaacagt ttattttacc actggacagt
     2401 ttgcttcact aaatgaaact actcaaattt gctgcaattg atataggatc gaacgctgtt
     2461 cgtttactcc tctcgaatgt tgtggaaaat ggtgatagtg tagtattccg caagtcctca
     2521 ctcattcgta tgcccattcg gttgggcgag gatgcctttg aaaccggtat catctccgaa
     2581 gcccgcattc agaagctgat taaaaccatg attgcctatc gcaacctaat ggaggtggag
     2641 gatgttgtgg cttaccgtgc ttgtgcaaca tcagccatgc gagaggcgct taatgcacct
     2701 gagattgtgg agcgtgtgag taggcaaacc ggagtaaata ttgaggtgat aaatggtagg
     2761 caggaggctg agattgttta ctcaaatggc attgccgaaa cgttagatag ttcaaaaccc
     2821 ttcatttatg ttgatgttgg gggtggcagc accgagattt ccctttttca aaacggacag
     2881 aatattgcct ctcaatcatt tgacatcgga acaattcgaa tgctgaagga aaaagtaggc
     2941 aaatcggagt ttgaccttat taagaagtgg ctaaaaaatc tgaaaattac cggcggaaag
     3001 cctaaaatta taggctcggg cggaaacatc aacaagctct accggctggc tggaaaggtt
     3061 gagggaaggc cgttgtcgtt tgccgagctt cagaagctat tcgcacagtt aaaatcctac
     3121 acctttgaag agcgtatcag catcctggga atgaaccccg atagggctga tgtgattatt
     3181 cctgccgcca ggatttacct gaaaacgatg gagtggaccg gggccagtaa aatactagtt
     3241 cccaccattg gcgtttcaga tggtattgtg cgaatgcttt acaagaatta ccggagtaga
     3301 agctagcgca ggttttctac ttttctatcc atacaaattg tgcaagcggt ttacttttag
     3361 gggcaatttg cacgctacga gccaatcatc aatctcgcca atgattgcta cctttgcaaa
     3421 aaataactga ataatggtgg agcagcgcaa acgggttgcc ttttacacgc taggatgtaa
     3481 gcttaacttt tccgaaacgt caaccattgc caggcagctt gtcgaaatgg ggtacgaaag
     3541 agtttcgccc gacgataagg ccgatgttta cgtgattaat acctgctcgg ttaccgagca
     3601 tgccgataag aaatgcaggc aagccatcag gaagtttgcc agtcgttcgc ccggcgccct
     3661 gatagccgta gtgggttgct acgcccagct taaacccgat gaggttgccg ccattgaagg
     3721 ggttgacctg gtagtaggcg cttcagccaa attccagctg ccccagctgc tagctacaca
     3781 tgtaggtaag gatggagcaa aagtttacag ctgcgatatt gacagggtag ccgatttttc
     3841 tccagcttac tcctcgggcg atcgaacccg ttcgtttctt aaggttcagg atggatgcga
     3901 ctaccattgc agctactgca ccataccgct tgccagaggg aaaagcagga atatgcctgt
     3961 taccaacatt gtggcacagg ctcaggcaat tgcagcacaa aacgttaagg aaatagttct
     4021 tacaggggtt aataccggcg atttcggtag gtcaaccggt gaaagtttca tagagctgct
     4081 aaaagagctc gatgccgttg agggtattga acgctacagg atctcctcca tcgagccaaa
     4141 ccttataacc gatgaaatag cccattttgt ggccagctca aaaaagtttc tgcctcattt
     4201 ccatattcct cttcaatcgg ggtcgaacag aatactcggg ctgatgcgcc gccgctacaa
     4261 ccgggagctc ttcgccgaaa aaattgccat gattcgccgg cttatgcccg atactttttt
     4321 tggggttgat gtgattgtgg gatttcccgg cgaaaccgaa gccgacttca tggacggcta
     4381 cagcttcata cgggaggtta accctgcatt cctgcacgtt tttccctact ccgaacggcc
     4441 caacacagct gccgctctgc tgccagctaa ggttgacgca agggaaatta aggttcgggc
     4501 tgaccgcctt ggcgcgctat ccagcgagct gcaccggcaa ttctacattt cccgaaccgg
     4561 ccaggttcac catgttttgc ttgaggcctc ccaaaaatcg gggctaatgt ttggcttcac
     4621 cgaaaactat atacgggtag ccgttccata taaccgtaat ttggctggaa aagtggttga
     4681 tgttcggtta gctgctctaa atcctaatgg gttcatggag ggtgagctta tgcaaccatg
     4741 aataaaccat gtaggggttt gcacagacgc gtatgaacaa ccggacgata tttccgagcc
     4801 cggcgaagcc aaaaatgagt gttcagcgcg ctcaatccag atatctaaaa ggtatgggca
     4861 aaatcagaat tcaaaaaatt ttttacggat aaaatggcat gctcatgctg ggtgctagct
     4921 aattggggta taatccgaat ttacccgaaa c
//
LOCUS       DAOPHX010000159         4950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_22761_length_4950_cov_6.000204, whole genome shotgun sequence.
ACCESSION   DAOPHX010000159 DAOPHX010000000
VERSION     DAOPHX010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22761_length_4950_cov_6.000204"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PK502_09735"
     CDS             complement(<1..867)
                     /locus_tag="PK502_09735"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPH01139.1"
                     /translation="MTQDDLFKKLISHCKEYGFVFPSSEIYDGLSAVYDYGQNGVALK
                     NNIRRFWWEAMTRVNENIVGIDSAIFMHPQIWVASGHTSAFNDPLIDNRDSKKRYRAD
                     TLIEEWIQKQEEKVEKEVEKARKRFGDSFDEQLFRQTNPRVVELSEHAKTVNQRMVQA
                     LEANDLAAIKKIIEDCEIACPISGTRNWTDVRQFNLMFETKLGSVSDEANTIYLRPET
                     AQGIFVNYLNVQKTGRMKIPFGIAQVGKAFRNEIVARQFIFRMREFEQMEMQFFVKPG
                     EEMKWFEYWLDKR"
     gene            1141..3717
                     /locus_tag="PK502_09740"
     CDS             1141..3717
                     /locus_tag="PK502_09740"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR/HmsC family protein"
                     /protein_id="HPH01140.1"
                     /translation="MFTRKVPVALAILCLLAFPVRPKSSEGDLPQVSEARVKAAVILN
                     IAREVRWPNERSIENFRVGVMDADTILLHELHKVEPSYLIRGKPFNTFYFSNESQLEN
                     IDILYLSSEFSKNLFRVYRELEGRPTLMITDGASDRALTMINLTYNAEKKAIAFEVNR
                     ENMESRGFRVGQELLLLGGSYMDIKELYVETSKRLNEATSLMAKYQEELDQLGRDHDE
                     YLHQIEMLDSQLTTLNYQLKKTEQDYSNLTLRLHDRDSLLGVRNRELQLRINESTLLQ
                     GLIQTQLDSIKNSSTLLDSLNQLVAEKQKELQAQQNLINNQGQVITEKESTILQQQRK
                     WYITLILLLGVLSSLIFASISYTTKRRMNLRLENLVEERTRELNASREHFKNLFENSP
                     VAMIELNFSALKDFSWQSDNGKVDIDSMSNEEFSALMSRALTYSRVVDANNAAIKLFG
                     YADKKDFMENPSKTLTFQSLVDYRSLAKLYFNNQPLAKFETTRQTTSGETIHAVFEWL
                     VLPGYENTYERVLLAITDITALKKYEKELTRHKDHLEEIVLERTREIIKLNEDLTSTN
                     AELKQKTEELTQTIQKLGEAQNQLVRSEKLASLGMLTTGIAHEINNPLNYISGSQQAL
                     GTLLEEMWKLLNSYRDIIATKLTSDVLINIEKEHNQNINDLYFSMKFLLSNIETGISR
                     ANSIVKSLRVFLGESSQEMAEYSIPNAVGSILTILKSSYFGKIRITEEYSPDLPLIHC
                     SAEGVNQVIMNLLTNAIDAIPEKGEINITATYEVDDDCIAVQVRDNGMGISKENINRI
                     FDPFFTTKETGSGTGLGLYLAYNIVIAHHGSIEVDSAEGVGTTFTLRLPRMVPNGLEL
                     PS"
     gene            complement(3706..4092)
                     /locus_tag="PK502_09745"
     CDS             complement(3706..4092)
                     /locus_tag="PK502_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPH01141.1"
                     /translation="MDGKEKILYVDDETINLELFKINFRNEYDVVVANSAIKGLEILK
                     GEPIKVVVSDLKMPVMNGLEFIEKVKGDNPARVCILLTAFMESDVMLQAINKELIFRY
                     IMKPWKKAELEEVIRLALVRHASLAG"
BASE COUNT         1434 a         1216 c         1064 g         1236 t
ORIGIN      
        1 ccgcttatcg agccagtact cgaaccattt catctcctcg ccgggcttaa caaaaaactg
       61 catttccatc tgttcaaatt cacgcatacg aaaaatgaac tgccgggcaa caatttcgtt
      121 ccggaaggcc ttgccaacct gggcaatacc aaaggggatt ttcatcctgc cggttttctg
      181 gacgttcagg tagttaacga atatcccctg ggcggtctcc ggacgcaggt atatggtgtt
      241 tgcctcatcg ctgacggaac ccagcttggt ttcaaacatc aggttaaact gccttacgtc
      301 agtccagtta cgggtgcccg agatgggaca ggcaatttca caatcctcaa ttattttttt
      361 tatcgcagcc aggtcattcg cttcaagcgc ctgcaccatt ctctggttta cggttttggc
      421 atgttccgac agctcaacca cacgggggtt ggtttgtcgg aaaagctgct catcaaaact
      481 atctccaaaa cgcttacgcg ccttttcaac ctccttttca actttttcct cctgcttttg
      541 aatccactcc tcaataagcg tatcggcacg gtagcgcttc ttgctgtccc tgttgtcaat
      601 cagcggatcg ttgaaggcgc tggtatgccc cgatgccacc caaatctggg ggtgcatgaa
      661 aatggcagaa tcaatcccca caatgttctc attaacccgg gtcatggctt cccaccaaaa
      721 gcgacggatg ttattcttta gcgccacgcc attttgtccg tagtcgtaca cggcgctcaa
      781 accatcgtaa atttcgctgg agggaaacac aaatccatac tccttgcagt gggatattaa
      841 cttcttaaaa agatcatcct gtgtcatata gcagctgtat attgttagct aggtaagagt
      901 aaaccgcaaa agtagctcaa attgccatcc tgtcaaattg aaaatttaca tcacccggaa
      961 catactgctc agcacaatta atcgtaaatc cctgtatgta aggcggaaag gaaaagcaac
     1021 caacaccgac gcttccattc ctggtttgca acttgtatga gcagggcata aatatacacc
     1081 tgcatgttga ctttcatcca ttgttttgta aattgcaggt aattcaccca gttttagaat
     1141 atgtttacaa ggaaagttcc cgttgcacta gccatacttt gtcttctggc ttttccggtt
     1201 cgcccgaaaa gttctgaagg cgacctgcct caggtatccg aggccagggt taaagcagcg
     1261 gttatcctta atattgccag agaagtcagg tggccaaacg agaggagtat tgaaaacttc
     1321 agggtagggg tgatggatgc cgataccatc ctcctccacg agctacataa ggttgagccc
     1381 tcatacctca tcagggggaa gccctttaac actttttact tctccaatga gagccagctg
     1441 gagaacatcg acatactcta cctttcatca gaattcagca aaaacctttt tagagtttac
     1501 cgggagcttg aaggcaggcc taccctaatg atcaccgacg gtgcctccga cagggcgcta
     1561 accatgatta accttacata caatgcagaa aaaaaggcta ttgcctttga ggttaatcgc
     1621 gagaacatgg aatcacgcgg cttcagggtt gggcaggagc tgcttctgct cggcggaagc
     1681 tacatggata tcaaggagct atacgtggaa acctcaaaac ggctgaatga ggcaacctcc
     1741 ctgatggcca aataccagga agagctggat caacttggac gcgaccacga tgagtacctt
     1801 catcagattg agatgctaga cagccagcta accactctga actatcaact caaaaaaaca
     1861 gagcaggact acagcaactt aaccctaagg ctgcacgata gggacagctt gctgggcgtt
     1921 cgcaacaggg agctccagct aagaattaac gagagcaccc tgctgcaagg gttaattcaa
     1981 acccagctcg attccattaa aaattcttca accctcctcg attcgctaaa ccagctggta
     2041 gccgaaaaac aaaaagaact acaggcccag caaaacctca ttaacaacca ggggcaggta
     2101 atcacggaaa aggagtccac cattctccag cagcagcgga aatggtacat caccctgatt
     2161 ttgcttttgg gtgttttatc aagcctgatt tttgcctcta tttcatacac caccaagcgg
     2221 agaatgaacc tgcggcttga gaatcttgtt gaggagcgca cccgggagct aaatgccagc
     2281 cgggagcatt ttaaaaattt atttgaaaac tcccccgtag ccatgataga gctgaacttt
     2341 tcggccctga aagatttctc atggcaatcc gataatggca aggtggatat cgacagcatg
     2401 agcaatgagg agttctcagc acttatgtcc cgtgccctta cctactccag ggtggttgat
     2461 gctaacaacg ccgccattaa actttttggc tatgccgata aaaaggactt catggagaat
     2521 ccctccaaaa cgctaacctt ccagtcgctg gttgattacc ggtcgctggc caaactctac
     2581 tttaataacc agccccttgc aaagtttgaa actaccaggc aaaccacttc aggcgaaact
     2641 atacatgctg tttttgaatg gctcgtgctt cccggctacg aaaataccta cgagagggtt
     2701 cttttggcta ttaccgatat tactgccctt aaaaagtatg agaaggagct tacccggcac
     2761 aaagatcatc tggaggaaat tgttctggaa cggaccaggg agattatcaa gctgaatgag
     2821 gatttgactt ccaccaacgc cgagctgaag cagaaaaccg aggagcttac acaaaccatt
     2881 caaaagctgg gcgaagcgca gaatcaactt gtacgttcag aaaaattggc ctcgctgggt
     2941 atgctcacaa cgggaatagc ccatgaaatt aacaaccccc taaactacat aagcggcagc
     3001 cagcaggcac taggaacact tctggaggaa atgtggaagc tgcttaacag ctaccgcgat
     3061 attattgcaa ccaagcttac cagcgacgtt ttaataaaca tcgaaaagga gcacaaccag
     3121 aatatcaacg acctttactt ctccatgaaa ttcctgctct ccaatattga aacgggcatc
     3181 agcagggcca actccatcgt taaaagcctg agggtgtttt taggtgaaag cagccaggaa
     3241 atggccgaat atagcatccc caatgcggtt ggaagtattc tcaccatttt aaaatcatcc
     3301 tactttggta agatcagaat taccgaggag tactccccag acctgccact catccactgt
     3361 agtgcagagg gggttaacca ggttatcatg aacctgctca ctaatgccat tgatgctatt
     3421 cccgaaaagg gcgaaataaa tatcacagct acctacgaag ttgacgacga ctgcatagct
     3481 gtgcaggtta gggataacgg catgggaata tcgaaggaaa atatcaacag gatattcgac
     3541 cccttcttca caaccaagga aaccggcagc ggaacagggt tagggcttta cctggcatat
     3601 aacatcgtga ttgcacatca tggctccatc gaggtggatt ctgctgaagg agttggaaca
     3661 accttcaccc ttcggctgcc acgcatggta ccaaacgggt tggagctacc cagctaggct
     3721 cgcgtggcgg actagcgcca acctgattac ctcctccagc tcggcttttt tccaaggttt
     3781 cattatatac ctgaatatca actccttatt aatagcctga agcataacat ccgattccat
     3841 gaaggcggta agcagtatgc aaacccgagc ggggttatcg cccttaacct tttcaatgaa
     3901 ctccagcccg ttcatcacgg gcatctttaa gtccgacaca accaccttta tcggttcacc
     3961 ttttaaaatt tccaacccct tgatggccga gttggccact accacatcat actcgttgcg
     4021 aaagttaatc ttgaacagct caaggttaat ggtttcatcg tcaacataaa gaattttctc
     4081 cttaccgtcc atgttacccg aaagttttta tcaatataaa caaatttact aaaatttagc
     4141 ttacaaggtt aatcggaatg atgattttta aaccttttat aaaagacgat taatgaaaag
     4201 gctacgccgg aaaggttaca aattaagtcg tacgggttgt aggtacgcga tgggattttc
     4261 agctggcaaa cttccaccaa ggctgctgtt gccaaaaaaa aaggaaatag aattaccggt
     4321 ttaatgcgta ggttggccag cgaagcagcg gcagtcaaca tcgcaaaaat caaaaaatgc
     4381 accaggtaat cggtatggaa atgaaaacca tcaaagggaa caaccggaag gctaaagtgt
     4441 aaagctgcag gaattaaaag ggtaatagta acccaaccta acagtataag gaataacttc
     4501 acccacttta cacgccataa atttttcatg gctcaaatat gacgaaaaaa atacattttg
     4561 gtagcagcac aggtaaaatc agcggtaaca agtaaatatt tgtcaccttt aaaaccctgt
     4621 ttcctaaaca cttagcctgt tgtgggtttt taccgttagc ttattcatgg gggatttctt
     4681 cccatcaacc tttatcctcg ttcttcagcc ccaacggggg aaagcattct ctgcaatgct
     4741 cgtaaaggcg ggaatcgttg aaagttgctg gaataagtaa aacgtcataa aagcctgacc
     4801 cgcaagtggg ccggtggctt aaatccgccg gtaggcggac aagttccgcc aggaggcgga
     4861 caggttcttg aatcttgaat tatcttatcc tttacccttt aacctttatc cttgtattaa
     4921 cctttatcct tttcttcctt gctagaacct
//
LOCUS       DAOPHX010000160         4939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_22825_length_4939_cov_5.512490, whole genome shotgun sequence.
ACCESSION   DAOPHX010000160 DAOPHX010000000
VERSION     DAOPHX010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22825_length_4939_cov_5.512490"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /gene="pyrH"
                     /locus_tag="PK502_09750"
     CDS             <1..330
                     /gene="pyrH"
                     /locus_tag="PK502_09750"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859761.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPH01142.1"
                     /translation="IIAGGTGNPFFTTDTASALRAVEINANILLKGTRVDGVYSADPE
                     KDPSAQRFESITFTEAYDKKLKIMDLTAFTLCSENNLPILVFDMNTPGNLKKAVFGDK
                     VGTLVKN"
     gene            362..928
                     /gene="frr"
                     /locus_tag="PK502_09755"
     CDS             362..928
                     /gene="frr"
                     /locus_tag="PK502_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007366899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPH01143.1"
                     /translation="MNPNDATATISTADEHMQKAIDHLENELRVIRAGRANPAILSGV
                     MVDYYGTMTPLSQVSSVGTPDARTIVVQPWEKNMINPIEKAIMAANLGFNPQNNGESI
                     RIMVPPLTEERRKELVKQVKHVGETARMSIRNARRDAIEEVKRLQKNGLPEDAAKDAE
                     AKIQKITDSFNKKVDELLDKKEKEILTV"
     gene            complement(1006..2208)
                     /locus_tag="PK502_09760"
     CDS             complement(1006..2208)
                     /locus_tag="PK502_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01144.1"
                     /translation="MSENDNLFLTLERLRVKLLAFPAADVTLFEKAKKKHISLFNPEQ
                     IEFVNDKPDILMFLTGGSEHIAIESVQEYRFYLMLASREANAWASATEVKAWMNMHNV
                     SSLLINTDDPLASTYIQEFYKASNGIRHLHGQRLGVVGKPSAWLVASTISPFLLQSRL
                     GIEQVDISWNEIMFDEIQQVSPDFTTLFGMADDKEELMASGRIFEGLASLIPFYRLNA
                     LSVECFPLVNQTSHTACLALAKLNLDGFPTACEADSCSAAGMMLANEVCGVIPWMANT
                     ILVEGNRAVFAHCTAPLNLLEEFKLDSHYETGKGYSIAGSLRSPDVTIFRLDNTLNKI
                     FITRAEVKSRPKSKTACRTQLEVEISDSAARYFTDSPFGNHHLILPNSYTMRLKLAAR
                     LLRMEVVE"
     gene            complement(2195..3376)
                     /locus_tag="PK502_09765"
     CDS             complement(2195..3376)
                     /locus_tag="PK502_09765"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPH01145.1"
                     /translation="MKRKQKIQLIALAVLAFLAIFLSLPQNRYIVKALIYQQVNIDDY
                     KIFHNRTVRMGQPQPWKISPQYNHYQLSDKQLEYFERYKTVAYLVVKDTAILYESYWD
                     GYGSDSYSNSFSMAKSIVSLLVGCALQDGYIRSIDQPVADFLPEFRQDGRDKITIKHL
                     LTMSSGLSWDESYSTLFSLTTKGYYGNNLAGLVLGQHVVEEPGKRFMYRSGDTQLLSL
                     VVEKASGETLSDYASKRLWSKIGAEHDALWCLDRQNGVEKAFCCFNSNARDFARFGQL
                     VLNQGTWNGEQLIPKDYLAEALQPATYLIDAETGKPCDFYGYQWWRINMDGHEVNYAR
                     GLLGQYIFVIPSLNAVVVRLGHLRGDETIEGQPEDVYQYLRCGVEIIKQLQIPSEYER
                     K"
     gene            3632..4747
                     /locus_tag="PK502_09770"
     CDS             3632..4747
                     /locus_tag="PK502_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01146.1"
                     /translation="MVSKRIGHKPKIAAALALVQLLAIQQFCYGQDSTSTLGLNLYSS
                     AFFDNKEFTGDIKKGYTLPGFFIQPVINFSTDNYSVEAGFHLLYMAGTDSLEHLVPVL
                     TLARKLSKSSTILVGTLDARNGHFLPEPLFKSERVFTSQPETGVQFKHKTNNTFSDLW
                     INWEQFIKTGSPFQEQFTVGFTFQYLESNSKTPVFFTKTMALATHQGGQIDSTNLPVT
                     TIVNIGETAGILIPLKPGNANLSIEVSGFLSADKSPNPHLKYDQGYAFYPRIQMEWPF
                     TRFSFGYWHARSFINPRGEELFGSLSTVNPTLDSKIRKLVTCSLEYSKTLQQGFTFGV
                     GVNGYYDTRQGLLDYSYTFRMIFDGELVRFRRTGASR"
BASE COUNT         1404 a         1189 c         1076 g         1270 t
ORIGIN      
        1 attattgccg gaggaacagg aaaccccttt tttactaccg acaccgcttc ggcacttcgg
       61 gcagttgaaa tcaacgctaa cattctttta aagggaaccc gggtggatgg cgtttactcg
      121 gccgaccccg aaaaagatcc ctctgcccag cggttcgaga gcataacttt tactgaagca
      181 tacgataaaa agctaaaaat tatggatttg accgccttta ccctctgtag cgaaaacaac
      241 ctccccatcc tggtttttga catgaacacc cctggaaact taaaaaaagc tgtctttggc
      301 gataaggttg gaactttagt taaaaattaa atacttttac aataaaatta acagtaagtg
      361 tatgaacccc aacgatgcaa cagcaaccat cagcactgct gatgaacata tgcaaaaagc
      421 cattgatcac cttgaaaacg agttacgggt aatacgcgcc ggcagggcta accccgcaat
      481 actttctgga gttatggtag actactacgg aacaatgacg cccctatctc aggtgtcgag
      541 tgtaggaacc cccgatgcga gaacaattgt agtgcaacca tgggaaaaaa acatgattaa
      601 cccaatagaa aaggctatca tggcagccaa cctggggttt aacccccaga acaatggtga
      661 gagcattcgc atcatggttc cacccctcac cgaggaacgc cgaaaggagc ttgttaaaca
      721 ggttaagcac gttggcgaaa ctgcccgaat gagcattcgc aatgcccggc gtgatgccat
      781 tgaggaggtg aagcggttac agaaaaacgg gttacccgag gatgccgcta aggatgccga
      841 agccaagatt caaaaaatta ccgattcctt caacaaaaag gttgatgagc tcctcgataa
      901 gaaggaaaag gaaattctta ccgtttagcc agccctacca cttgacctgt ccgataagat
      961 tacttttctg tttctgccaa actctaagtt catagccctt ttgggctact ctaccacctc
     1021 cattctcaaa agtcgggcag ccagctttaa ccgcatggtg tagctgttgg gtaaaatcag
     1081 gtggtggttt ccaaagggtg aatcggtaaa gtaccgggca gccgaatcgg aaatttcaac
     1141 ctccagctgg gttctacagg ccgttttaga ttttggtctg ctctttactt ctgccctggt
     1201 gatgaaaatc ttgttcaggg tgttgtccaa cctgaaaata gttacatccg gagacctaag
     1261 gctaccggca attgaatacc ctttgcccgt ttcatagtgt gaatcgagtt tgaactcctc
     1321 caacaggttg agaggtgcag tgcagtgggc aaaaaccgcc cgattacctt cgaccaaaat
     1381 ggtattggcc atccagggga ttaccccgca cacctcgtta gccagcatca ttccagcagc
     1441 actacagcta tcggcctcac atgccgtagg aaatccatca agattaagct tggcaagggc
     1501 taaacatgct gtgtggctgg tttggtttac cagcggaaag cactcaaccg aaagggcatt
     1561 aagcctataa aaggggataa gggaagccaa cccctcgaat attcttccgc tggccatgag
     1621 ctcctccttg tcatcagcca tgccaaacag agttgtaaaa tccggcgaca cctgctgaat
     1681 ttcatcgaac atgatttcat tccacgaaat gtcaacctgc tctataccca gcctgctctg
     1741 aagtaaaaaa gggcttatcg tagaagcaac aagccatgct gacggcttgc caactactcc
     1801 aagacgctgc ccgtgtaaat gacgtattcc atttgatgcc ttgtagaact cctggatgta
     1861 ggttgaagct agcgggtcat cggtgtttat aagcaaggat gaaacattat gcatgttcat
     1921 ccacgcctta acctccgttg ccgacgccca ggcgttggcc tcccgtgaag ccagcatgag
     1981 gtaaaaccgg tactcctgaa ccgattcaat agcaatatgc tcagaacctc cggtaagaaa
     2041 cattaaaata tcgggtttgt cattcacaaa ctcaatttgc tcggggttaa aaagggatat
     2101 atgctttttc ttagcctttt caaatagcgt tacgtcagct gcaggaaagg ccagaagctt
     2161 aacccttagc cgttccaaag tcaggaacag gttgtcattt tcgctcatac tcgcttggta
     2221 tttgtaattg cttgatgatt tcaactccgc aacgaaggta ttggtaaaca tcctcgggtt
     2281 gcccttcgat tgtttcgtcg cctcggaggt gacctaggcg aacaaccacc gcattaaggc
     2341 tggggattac aaatatgtac tgacccagca aaccccgggc gtagtttacc tcatgcccat
     2401 ccatgttaat acgccaccac tggtagccgt aaaaatcgca gggttttccg gtttcggcat
     2461 caatcagata ggtggcgggt tgtagcgctt ctgctagata gtcctttggg attagctgct
     2521 ctccattcca agtcccctgg ttgagcacga gttggccaaa gcgggcaaaa tcgcgggcat
     2581 tcgagttgaa acagcaaaag gccttctcaa caccgttttg cctgtcgagg caccagagtg
     2641 catcgtgctc tgcaccgatt ttcgaccaca aacgttttga agcatagtca gaaagagttt
     2701 cccctgaagc cttttctaca accaacgata gcagctgggt gtcgccgcta cggtacataa
     2761 accgtttacc gggctcctca accacgtgct gtccaagaac cagtcctgca aggttgtttc
     2821 catagtaacc ctttgtggtt aaagaaaata aggtgctgta cgactcgtcc cagcttagcc
     2881 ccgagctcat ggtaagtaga tgcttaatgg taatcttatc ccggccatcc tgacggaatt
     2941 cgggaagaaa atcggcaacc ggctggtcaa tactccttat gtagccatcc tgaagggcgc
     3001 aacctaccag caagctaaca atgctcttgg ccatagaaaa tgagttggag taggaatctg
     3061 agccgtaacc atcccagtaa gactcataaa gtatggcggt atctttcacc acaaggtagg
     3121 caacagtttt atacctctca aagtactcca gctgcttatc cgatagctga tagtggttgt
     3181 actgaggaga tattttccat ggttgcggtt gccccatacg gaccgtccgg ttatggaaaa
     3241 ttttatagtc gtcaatattt acctgctggt aaattaaagc tttaacaatg tagcgatttt
     3301 ggggtaggct aagaaaaatg gccaaaaagg ccagcaccgc caacgcaata agttgaattt
     3361 tttgcttgcg cttcatgggt ttgcttttag tgttatggtc aatgttaaaa aaatcgtccc
     3421 tccctaagga gtttacctat aataagatga taaaccctaa cagctagaag aaccaaactg
     3481 cctcaaccta taagcatcat catctaaaca gttgtaaacc ttaaagatag tcaaaatcgg
     3541 attacatcaa attagcaata aaactaggaa aaactagcaa aaaagagcat acctttaccc
     3601 gattttcaat tagataaact ttttattcat catggtaagc aaacgaattg gtcataagcc
     3661 aaaaattgca gctgcgttag ctctcgtgca gctacttgca attcaacagt tttgctacgg
     3721 acaggatagc acctctacac taggcttaaa cctttactcc tcagcctttt ttgacaacaa
     3781 ggagttcacg ggtgatataa aaaagggcta cacactgccg ggattcttta tacaacccgt
     3841 tattaacttc agtaccgata actatagcgt tgaagcgggt tttcaccttc tatatatggc
     3901 cggtaccgat agcctggagc atttagtccc tgtcctgaca cttgcacgaa agctttccaa
     3961 aagctctacc atcctggttg gcacactgga tgcccgaaat ggccatttcc ttcctgaacc
     4021 actttttaag tccgaacggg tgtttaccag ccaacccgaa accggggttc agtttaagca
     4081 taaaacgaac aataccttca gcgacttgtg gattaactgg gagcagttca taaaaacggg
     4141 tagccctttt caggagcagt ttacggtggg atttaccttt cagtatctgg aatcaaacag
     4201 taagaccccc gtctttttta ctaaaacaat ggcattggct acccaccagg gtggtcagat
     4261 agacagcacc aatcttccgg ttaccacaat tgttaacatt ggagaaacgg caggtatact
     4321 aattcctctc aaaccgggca atgccaatct tagcatcgag gtttcaggtt ttctttcggc
     4381 tgacaaatcg ccaaatcccc accttaaata tgatcaaggc tacgcgtttt acccaaggat
     4441 tcaaatggaa tggcctttta cccgcttttc atttggttac tggcatgcca gatcatttat
     4501 taatcccaga ggtgaagaac tttttggttc cctgtcaact gttaacccta cactcgattc
     4561 aaagattcgc aagctcgtaa cctgttcgct ggagtacagt aagaccctcc aacagggttt
     4621 tacctttgga gttggagtaa atggctacta cgataccagg caaggattgc tggactacag
     4681 ctacaccttt agaatgattt tcgacggaga gctagtacgg ttcaggagaa ctggggcatc
     4741 tcgttgacaa ctcgaaaaga cccgttagaa ctgaacctgt taattgttaa cagtcgaaat
     4801 ttagcgttga acttaatatt ccacgaaagc ctgtcattgt tttaagtccg tcaaaacttg
     4861 aaaatattct ttgctttttc atttttcagc cccaacgggg tgcaataagc agcaaggggc
     4921 aaagcccctt gataaggac
//
LOCUS       DAOPHX010000161         4865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_23300_length_4865_cov_4.620582, whole genome shotgun sequence.
ACCESSION   DAOPHX010000161 DAOPHX010000000
VERSION     DAOPHX010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23300_length_4865_cov_4.620582"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PK502_09775"
     CDS             complement(<1..188)
                     /locus_tag="PK502_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01147.1"
                     /translation="MKAFLRISVTLFLLLSSLSGYSASLELSKQYDREFKVSPASKLY
                     VENKYGQISIENWDKSAIA"
     gene            complement(236..751)
                     /locus_tag="PK502_09780"
     CDS             complement(236..751)
                     /locus_tag="PK502_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01148.1"
                     /translation="MDKLEKFFAENRGQLDDVDLPDGHEQRFKSRIHAQKNHTTIKTF
                     ALAAASVSIILALTAALSLMYGNHFSEELSRHFSKGSTAQFMEVEAYYRSQLLQKYRA
                     IEKLASSTDPSVKPEAKQLLEEFDREGQRLKAELPKNPRSDMVLGAMYENYQSRLEVL
                     QKIEGTLDKGK"
     gene            complement(763..1290)
                     /locus_tag="PK502_09785"
     CDS             complement(763..1290)
                     /locus_tag="PK502_09785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPH01149.1"
                     /translation="MDISSELVKKCCRGDSRAQFELYSMYSRAMYNVSCRIIPDRMEA
                     EDAMQEAFFKAFDRISTFRGEVTFGAWLKRIVINTCLDHLKKRRVQLVSFDDLPGLAE
                     HTDSSDDYTPESVEQVKLAMQQLPEGYRVVLTLRLVEELDYPEIAEMLDINESSVRSQ
                     YTRGRQKLVELLKRN"
     gene            complement(1503..2666)
                     /locus_tag="PK502_09790"
     CDS             complement(1503..2666)
                     /locus_tag="PK502_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPH01150.1"
                     /translation="MEQSGGFFSFLVENLNQFLSFTGFANFTIGHGVMILVGLFFIYL
                     AISKEFEPMLLVPIGFGIIVGNIPFSPGYQIGVYESGSVLNYLYFGVLNGIYPPLIFL
                     GIGAMTDFSALISNPKLMLIGAAAQLGIFGAYILALVLGFSPQEAGAIGIIGGADGPT
                     AIFLSSKLAPELLGAIAISAYSYMALVPVIQPPIMRLLTTRKERVIRMKPPRQVTKLE
                     KILFPIVGILLTAFIVPSGLPLLGMLFFGNLLKESTVTKRLAETARGPLIDIVTILIG
                     LTVGASTQATTFLTAKSLEIFIIGAISFIVATFGGVMFVKIINLFLKEGQKINPLIGN
                     AGVSAVPDSARVSQVIGLEYDKTNHLLMHAMGPNVAGVIGSAVAAGILLSFLA"
     gene            complement(2669..3106)
                     /locus_tag="PK502_09795"
     CDS             complement(2669..3106)
                     /locus_tag="PK502_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPH01151.1"
                     /translation="MKDFKFKINGNEYSVHIANVDGNLADVEVNGTPYRVEMERELRQ
                     TKTPKLIRQEAVPATDSHPSVVKTVSPDSPSTGMVKSPLPGVVLDVLVKPGDEVKVGQ
                     RLIVLEAMKMENNIDSDKAGRVTEVKVRKGDTVLEGEVLIIIG"
     gene            complement(3148..4071)
                     /locus_tag="PK502_09800"
     CDS             complement(3148..4071)
                     /locus_tag="PK502_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HPH01152.1"
                     /translation="MMKKFIHGILAALLWMGAAVSSTAQSVYDLRINEVMLFNTENYI
                     DSYGQRSSWIEIMNLGYNTVNIGGCYLTDDPTQPKKYLIPKGDPVTRIPQQQFLVFFA
                     NGKSHHGVLHLNFTLDSTHRFVALYSSDGRSLIDSVTVPLSLPNTSYCRIPDGTSTWQ
                     ITSFTTPNATNNLFTHEETSGEKFVQFDPFGIMMTCIAMLVVFTALFILYRIFRFLGM
                     MMQKPLRVGKRESRDVGKTREVQVAELSGEILAAVSAALYLYETEQHDQESEIITIQR
                     VSRHYSPWSSKLYSLTQQPNRVVTHRVHNKQ"
     gene            complement(4094..>4865)
                     /locus_tag="PK502_09805"
     CDS             complement(4094..>4865)
                     /locus_tag="PK502_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPH01153.1"
                     /translation="IDRLEDSLNDIIPDNPGKPYDVKDVITAIVDYGEFLEVQRDYAH
                     NIITCFARFNGQSVGIVANQPKYLAGVLDINSSRKAARFVRFCDSFNIPLVTLVDVPG
                     FLPGTGQEYNGIIIHGAKLLYAYGEATVPKITIILRKAYGGAYDVMSSKHLRGDINYS
                     WPTAEIAVMGPKGAIEILYSKELETLEDQKAKDDFLLRKEEEYKNKFANPYVAAKYGY
                     IDDVIEPRNTRFRVIRALQSLATKKVVNPPKKHSNLPL"
BASE COUNT         1324 a         1126 c         1060 g         1355 t
ORIGIN      
        1 gctatagcgc ttttatccca attctcaatg ctaatttggc catacttgtt ttcaacgtaa
       61 agtttagatg caggcgaaac cttaaactcc ctgtcgtatt gtttagaaag ctcgagggat
      121 gcggaatatc cgcttaagct gctcagtaat aagaatagag ttacagaaat tctgagaaat
      181 gctttcatgg ttttttatct attaagttaa attattaaca tggcaaaaag tgctgctact
      241 tccccttatc aagtgttcct tcaatttttt gtagaacttc cagccttgac tggtagtttt
      301 cgtacattgc tccaagcacc atgtcgcttc ggggattctt tggtagttct gctttaaggc
      361 gttgaccctc cctatcaaac tcctcaagga gctgcttggc ttcaggtttt acagaaggat
      421 ccgttgatga ggcaagtttt tcaatcgccc tgtatttttg gagcagctgg ctccggtagt
      481 aggcttctac ttccatgaac tgtgcagttg accctttaga aaaatgccta gaaagctctt
      541 cagaaaagtg gttgccatac attaggctca atgcagcggt tagtgcaaga atgatggaaa
      601 ccgaagcggc agctagtgca aaagttttaa tggttgtatg gtttttttgg gcatgaattc
      661 gggacttaaa ccgctgctca tgcccatcgg gtaggtccac atcgtcaagt tgacctctgt
      721 tttcggcaaa aaatttctca agcttatcca ttgtattgcg tgttagttcc tttttaaaag
      781 ttcaaccagc ttttgacggc cacgcgtgta ttgcgatctt accgatgact cattaatgtc
      841 aagcatttcg gcaatttcag ggtaatccag ctcttcaact aagcgcaggg tcagcaccac
      901 cctatatccc tcgggcagct gctgcatagc gagcttaacc tgctcaaccg attcgggggt
      961 ataatcgtcg ctactgtcgg tatgctcggc taagccaggc aaatcgtcga acgaaaccag
     1021 ctgcaccctt ctttttttaa gatggtcgag acaggtgttt atcacgattc gcttaagcca
     1081 agcgccaaaa gtaacctcgc ccctgaaagt actaatcctg tcgaatgctt taaagaacgc
     1141 ttcctgcatg gcatcttcgg cttccatcct atcgggaatg atgcggcagc ttacattata
     1201 catcgctctg gagtacattg aataaagttc aaactgcgca cgactatcgc ctcggcaaca
     1261 cttttttaca agctcggagc taatatccat tcctaatcag gctaaaaacc aaatacaacc
     1321 gaaagacttt cccatggatg cgtttgctgc atgggcaggg tattttttca aatttaggtt
     1381 taatttttct ataaaaatac ccaagcatgt ttgtgtcaga acagtaataa aacaaacaaa
     1441 aagcctgatt gcctataagt tggcaaatca ggcttttagc aattgggtta tgtagctcta
     1501 cactatgcca gaaagctcaa aagaatacca gcggcaacgg cgctaccaat aactccggcc
     1561 acattgggcc ccatggcgtg catcagcagg tggttggttt tatcgtactc caacccaata
     1621 acctgcgaaa ctctagcgct atcaggaaca gctgaaaccc cggcatttcc gattaagggg
     1681 ttaatttttt gcccctcctt caggaaaagg ttgataattt taacaaacat taccccacca
     1741 aaagtagcca ctataaacga aatggctcca attataaaaa tttcgagtga ttttgcagtt
     1801 aagaaggtgg ttgcctgggt agaagctcct actgttagcc caattaggat ggtaacaata
     1861 tcaataaggg gtcctctggc cgtttcggca agccttttgg ttactgtgct ttccttaagc
     1921 aggttcccaa agaatagcat tcccaaaagc ggcagccccg aaggaactat gaaggcagtg
     1981 agaagaattc ccacaattgg gaacaagatt ttctcaagct tggtaacctg tctcgggggt
     2041 ttcatacgaa taacgcgctc cttcctggtg gtaagcaacc tcattatagg aggttgaata
     2101 acaggcacca gagccatgta ggagtatgct gaaatggcaa tagcacccag tagctccggc
     2161 gcaagttttg atgaaaggaa aatggctgta ggtccgtcag caccaccgat gattccaatt
     2221 gcaccggctt cctgtggaga aaatcccaac accaaggcaa gaatataggc accaaaaata
     2281 cccagctgtg ctgcggcacc aatcagcata agcttagggt ttgatatgag cgcgctaaaa
     2341 tcggtcatag cgccaatgcc cagaaaaatg agtggcgggt aaattccatt tagaacgcca
     2401 aagtacaggt agttaagcac gcttccactt tcataaacac caatctggta gccgggtgaa
     2461 aaagggatat tacccacaat gattccaaag ccaataggga caagcagcat gggttcaaat
     2521 tcctttgaaa tggccaggta aataaagaat aaacccacca aaatcattac cccatgtcca
     2581 attgtaaagt tggcaaaacc tgtaaatgag agaaactggt tcaggttttc cactaagaat
     2641 gaaaaaaatc ctccgctttg ctccatggct atccgataat tattagaacc tctccctcca
     2701 gcacggtatc gccctttcta acctttacct cggttactct tccggcctta tcggagtcaa
     2761 tattgttctc cattttcatg gcttcaagaa caataaggcg ctgccccacc ttaacctcgt
     2821 cgccgggttt aaccagcaca tccagcacca cgcccgggag cggagacttt accatacccg
     2881 tagaaggtga atcgggacta accgttttta ctactgatgg atgtgagtcg gtagctggta
     2941 ctgcttcttg cctgattagc tttggcgttt tagtttgcct taactccctt tccatttcaa
     3001 ccctgtaggg tgttccgtta acctctacat cggcaagatt gccatcaaca ttggcaatgt
     3061 gaacgctgta ctcattgccg tttattttga atttaaaatc cttcattctc gtctgacatt
     3121 aagttttact ttttcttctg gtgtgttcta ctgtttgttg tgtacgcggt gggtaactac
     3181 acggtttggc tgctgcgtta agctgtatag ttttgaactc cagggagagt aatgtctcga
     3241 aacccgctga atggtgatta tttccgattc ctgatcgtgc tgttcggttt cataaaggta
     3301 aagagcagca gaaactgcag caagaatctc tcccgatagt tcagccacct gcacctcccg
     3361 ggttttacca acatccctac tttccctttt tcctacccta agaggttttt gcatcatcat
     3421 tcctaaaaac ctaaatattc ggtaaagaat aaaaagggca gtaaacacca ccagcatagc
     3481 aatgcaggtc atcataattc cgaaggggtc aaactggaca aatttctcgc ccgatgtttc
     3541 ctcgtgtgta aaaaggttgt tggtggcatt gggtgtagta aaactggtta tttgccaagt
     3601 gctagtcccg tcgggtatcc tgcaatacga ggtattcggc aacgagaggg gaacggtaac
     3661 gctgtctata aggctccttc catcggagct gtaaagggca acaaacctgt gggtgctgtc
     3721 gagtgtaaaa ttgagatgga gcacaccatg atggcttttc ccgttggcaa aaaatacaag
     3781 aaactgctgt tggggaattc tggttaccgg atcacccttg gggataaggt atttttttgg
     3841 ctgggtaggg tcgtcggtaa ggtagcaacc accaatgtta acggtgttat agcccaagtt
     3901 cataatttca atccatgaac ttctctggcc atagctatca atgtagttct cggtgttaaa
     3961 gagcattacc tcgttaatgc gcaaatcgta aacactctgc gcggtagatg aaacagctgc
     4021 tcccatccac agtagggctg caagtatgcc atgtataaac ttcttcatca tgccaaatta
     4081 ttatttcgaa attctacaac ggcaggttag aatgcttctt gggcggatta accacctttt
     4141 ttgtagccag cgactgtagt gccctaataa ctcggaaccg ggtgttgcgc ggttcaatta
     4201 catcatcaat atatccgtat tttgcggcaa cgtagggatt tgcaaacttg ttcttatact
     4261 cttcctcctt gcgtaacaag aaatcatctt tggctttctg gtcttccaac gtttccagct
     4321 ccttgctgta aagaatttca atggcaccct tggggcccat aacggcaatt tcggcggttg
     4381 gccaggagta gttaatgtcg cctctaaggt gcttggagct cataacatca tatgcaccac
     4441 cataggcttt ccgaagaatg atggtaattt tgggaacagt agcctcaccg taggcatata
     4501 gcagctttgc cccatgaata atgatgccgt tgtactcctg tcctgttcct gggagaaaac
     4561 ccggcacatc caccagcgtt accagcggaa tattgaaaga gtcgcaaaag cgaacaaacc
     4621 ttgcagcctt tcttgaagaa tttatatcaa gcactcctgc caggtactta ggctggttgg
     4681 ccacaatacc caccgattga ccgttaaatc gggcaaaaca agtaataatg ttgtgggcat
     4741 aatcgcgctg aacctcaaga aactcgccat agtcaacaat tgcagtaata acatccttaa
     4801 cgtcgtaggg cttacctggg ttatcgggaa taatatcgtt gagggaatcc tcaaggcggt
     4861 caatt
//
LOCUS       DAOPHX010000162         4832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_23547_length_4832_cov_5.559766, whole genome shotgun sequence.
ACCESSION   DAOPHX010000162 DAOPHX010000000
VERSION     DAOPHX010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23547_length_4832_cov_5.559766"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PK502_09810"
     CDS             complement(<1..807)
                     /locus_tag="PK502_09810"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPH01154.1"
                     /translation="MIRFEEAYALVLSKVVTIQKPERVLLLESLHRVLAEDIVSDINM
                     PPFRKSAVDGFACRMSDLRMPLKIVETIAAGSLPTMQVEPGTCAKIMTGAPIPLGADC
                     VLPVEDTRLADDLYVEFTGMEAKKNICELGEDIRIGDVSVNKGIIIGPQHIAIMAALG
                     YHQPLVATQPRVGILPTGDELVEPNIKPLGGQIRNSNGYQLVAQTKAAGALPSYYGIV
                     SDSKDATYEVLAKAIAENEVVTLTGGVSMGDYDYVPEIMEKLGVTIHFSSV"
     gene            963..1520
                     /locus_tag="PK502_09815"
     CDS             963..1520
                     /locus_tag="PK502_09815"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPH01155.1"
                     /translation="MLKDRGYYIDLLHQHISNPKMIAHCLASEAVLRALARKFGEDEE
                     VWGIAGLVHDIDSEQTESDHTMHGLVAAEMLNGVLPDEAVDAVRMHNEDATGMERSTK
                     FQHALAAGETITGLIFATALVYPDRKISSVKPKSVVKRMKEKQFAASVNRSTIMECEL
                     LGLSIDDFVSLSLEALAPIESELGF"
     gene            1767..2573
                     /locus_tag="PK502_09820"
     CDS             1767..2573
                     /locus_tag="PK502_09820"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPH01156.1"
                     /translation="MKLELICEQVVTEAKKAGSFAMEQRANFNINKVEVKGKHNFVSY
                     VDRTCEAMLVDGLSKIVPQAGFIAEEGTGKPNKGGLNWVVDPLDGTTNYIHGISPFAV
                     SIALMDDQKLLLGVVYEANLKECFWATADSQAYLNGAPIKVSETGKVNDALVATGFPY
                     YDYSRINPFFQTMEYFMRNSHGLRRLGSAATDLVYVACGRFDAFYEYGLSPWDVAAGA
                     LIVERAGGRVCDFKRGNDFIFGGEIMATNSSLYGEFADILNGMMSKSQSE"
     gene            2655..3569
                     /locus_tag="PK502_09825"
     CDS             2655..3569
                     /locus_tag="PK502_09825"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPH01157.1"
                     /translation="MKRLAFYLFYTITKILALLPLRLLYVISDINRFLLYYVLRYRRK
                     TVTTNLKNAFPDKSKEELKAIEWKFYRHLCDLFAEIIFLLHSGAKRAAKLCTFSNVEL
                     VNSYYQQGKSVVAAGGHFGNWELYGLFPLYIKHTVLGAYKPLNNKPIDEFIKKSRERF
                     GCVTIPMYSISRVALKWSQEGKPFFLGLINDQSPSRSHIGYWTTFLNQETPVYIGTEK
                     IARKINQPVVFCYIRKLKRGKYEANVELLVEEPRLTKPHEITEMHLRALERVINEAPE
                     YWLWSHKRWKHKRDVTTNSLQAGNEHTP"
     gene            3553..4581
                     /locus_tag="PK502_09830"
     CDS             3553..4581
                     /locus_tag="PK502_09830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPH01158.1"
                     /translation="MSIHHEVAVVILNWNGEKFLQRFLPGVLANSNETGVAVYVADNG
                     STDGSIKILRSRFPLVNLILLDRNYGYAGGYNRALSQIDSDFFVLLNSDVEVPGGWLN
                     PLRSFMRSNPNAAACAPAIMDIARPECFEYAGAAGGFIDKFGYPFCRGRILSEIEQDT
                     GQYNQSIEVFWASGACMMVRANTFWDAGGFDEHFFAHMEEIDLCWRMLNMGYSVWSIP
                     ESKVYHVGGGTLPNNNPYKLYLNYRNSLFMLHKNLSKNAFYQTIIARLLLDWLSAMAY
                     LVRFQLKFFGAVAKSHYHYYRDISRLNSFRSSFRHPKTNSAKVIYHRSVLFDFFILRR
                     RKFSELKF"
BASE COUNT         1270 a         1062 c         1161 g         1339 t
ORIGIN      
        1 tacgctgctg aagtggatgg taacccccaa cttctccatg atttcgggca cgtagtcgta
       61 atcgcccatt gaaacgccac ctgttagcgt aacaacctcg ttttcggcaa tggctttggc
      121 tagaacttcg taggtagcat ccttgctatc actcacaatg ccatagtagc tgggcaaagc
      181 tccggcggcc ttagtctgtg caaccagctg atagccattg ctgttcctta tctgcccacc
      241 cagtggtttt atattaggct ccaccagctc atcgccggta ggtagtatgc ccactctggg
      301 ctgagtggca accaatggct ggtggtatcc cagtgctgcc ataatggcaa tatgctgtgg
      361 accaataatt atgcctttat tgacagatac atctccaatc cgaatatcct cacccagctc
      421 gcaaatattt tttttggcct ccatgcctgt gaattcaacg tacaaatcgt ctgccaggcg
      481 ggtgtcctca acaggtaaaa cgcagtcggc gccaagcgga attggtgccc cagtcattat
      541 tttggcgcaa gtaccgggtt caacctgcat ggtgggcagg cttccggcag caatggtttc
      601 tactatcttt aagggcattc ggaggtcgct catcctacag gcaaagccat caacagccga
      661 ttttctgaaa ggcggcatgt taatgtccga aactatatcc tcagcaagca ccctgtgcag
      721 gctctccaac agtaaaactc tttctggctt ttgaatggta actactttgg agagaaccaa
      781 ggcgtaagct tcctcaaacc taatcatggt cagggttaat tttccacaaa gttgttaaaa
      841 aaatcattac cagggtaact gttttaggct tttttgcctg ctcatccgta atagcctgct
      901 taattaataa agcgctggtt agctattgct aaaattgtta attttgacaa aaattttgta
      961 ggatgttgaa ggatcgcggt tactatatcg acctgcttca ccagcatatt tcaaacccca
     1021 aaatgatagc tcattgtcta gcctctgagg cagtgctaag agccttagcc agaaagtttg
     1081 gtgaggatga ggaagtttgg ggaattgccg gtttggttca tgatattgac tctgagcaaa
     1141 ccgagtcgga ccacaccatg catgggctgg tggctgccga gatgctaaac ggcgtgctac
     1201 ccgatgaggc ggttgatgct gttcggatgc acaacgagga tgctaccgga atggagcgta
     1261 gcaccaagtt ccaacatgcg ctggcagccg gtgaaaccat taccgggcta atttttgcca
     1321 ccgcgctggt ttaccccgac aggaagatta gcagcgttaa gcctaagtcg gttgtcaaaa
     1381 gaatgaagga gaagcagttt gccgcctctg tgaatcgtag cactatcatg gagtgtgaat
     1441 tgttagggct ttcgattgac gatttcgtca gcctttcgct ggaggcactt gctccaattg
     1501 agtcggagtt gggcttttag agtttatcag gcatgggctt gctgctttaa taactggaga
     1561 aaattttttc agaaattcct cctgaatacc tttaaagttt tggaaagctg catgctgatc
     1621 aaattgatgc tggtgaatta catagctaaa atacggacta atttgcatta atcatcttag
     1681 ctatgcgctt gcatgctata tgatatgtat gcaggttgaa agttattttt ttgttggcat
     1741 aaacacttat taagctgaat attaacgtga agttagagtt gatttgtgaa caggttgtta
     1801 ccgaagccaa aaaggcgggt agctttgcca tggagcaacg ggcaaacttc aacatcaata
     1861 aggttgaggt gaaaggcaag cataattttg tatcgtatgt tgaccggaca tgcgaggcga
     1921 tgctggttga tggtctctcg aaaattgtcc ctcaagccgg ctttattgct gaggagggaa
     1981 ccggtaagcc aaataaaggg gggctgaact gggtggtgga tcccctggat gggacaacca
     2041 actacattca tggaatatcc ccttttgcgg tcagtattgc cctgatggac gatcaaaaac
     2101 tgttgctggg cgttgtgtat gaggccaact taaaggaatg cttttgggca actgccgaca
     2161 gccaggccta tttaaatggt gcacctatta aagtgtcgga aaccggtaag gtgaacgacg
     2221 cccttgttgc aaccggtttt ccctactacg actactcccg catcaacccg ttttttcaga
     2281 ctatggaata cttcatgcgt aattcccatg gtttgagacg gttaggctct gctgctaccg
     2341 atttggttta cgtggcctgc ggacgattcg atgccttcta cgagtatggg ctaagccctt
     2401 gggatgtggc tgccggagct ttgattgtgg agcgtgccgg aggtagggta tgcgacttta
     2461 agcggggtaa tgattttatt tttggaggtg aaataatggc taccaacagc agcctatatg
     2521 gtgagtttgc cgacatctta aacggcatga tgagcaaatc ccaatcggaa taagttaaac
     2581 atctatgaaa gaagctcaac tattacagcc accggcttca tccgcgtcaa tcataaattt
     2641 ttaaaaattg acagatgaaa cgactagctt tttacctgtt ttacaccata acaaaaattt
     2701 tagccctgct accgctaaga ttgctctacg taatttccga cattaacagg tttttgcttt
     2761 actacgtgct gaggtacagg cgaaaaacgg tgacaactaa tttaaaaaat gcctttccgg
     2821 acaaatcgaa agaggagcta aaggcaattg aatggaagtt ttaccggcat ctgtgcgact
     2881 tatttgccga aataattttt ttactgcatt cgggggcaaa gcgggcagct aagctttgca
     2941 cctttagtaa cgtggagctg gtgaatagct actaccagca ggggaaaagt gtggtagcgg
     3001 ccggtggtca tttcgggaac tgggagcttt acggcctgtt tccgctctac ataaaacaca
     3061 ctgttctggg tgcgtacaag ccacttaata acaagccgat tgatgaattc atcaaaaagt
     3121 cacgtgaacg ttttgggtgt gttaccatac caatgtacag catttcgaga gttgccctaa
     3181 aatggtcgca ggaaggtaaa cccttctttt tggggcttat aaacgaccaa tcgccatctc
     3241 gaagccatat tgggtactgg actacttttt tgaaccagga gactccagtt tatattggga
     3301 ccgaaaagat agcccggaaa ataaaccaac ccgtggtgtt ttgttacatt cgcaagttaa
     3361 agcgaggcaa gtatgaagcg aacgtggagc ttctggtcga agaacctagg ctgactaaac
     3421 cccacgagat taccgagatg cacctccgag cgttggaaag ggtgattaat gaagctcctg
     3481 agtactggct ctggtctcat aagcgatgga agcataaacg tgatgtaacc actaactcct
     3541 tacaggcagg aaatgagcat acaccatgag gttgccgtag ttatcctgaa ctggaatggc
     3601 gagaagttcc tgcaacgctt tcttccggga gtactagcca actccaacga aactggcgtg
     3661 gcggtttatg ttgccgataa tggttctaca gatggctcaa ttaagattct cagatcaagg
     3721 tttccgctgg taaacctcat cctactcgac aggaattatg gctacgctgg agggtataac
     3781 cgtgcgctaa gccagataga cagcgatttt tttgtgctgc ttaattctga tgttgaagtg
     3841 cctgggggat ggcttaatcc tttacgtagc tttatgcggt caaacccaaa tgcagccgct
     3901 tgcgctcctg ccattatgga cattgcccgc ccggagtgct ttgagtatgc cggagcggcg
     3961 ggtggtttta ttgacaagtt tggctacccg ttttgtaggg gtaggattct atccgaaata
     4021 gagcaggaca ccgggcagta caaccagtcc atcgaggtat tctgggcaag cggggcctgc
     4081 atgatggtga gggcaaacac tttttgggat gcgggcggtt tcgatgaaca tttttttgcc
     4141 cacatggagg aaattgacct ctgctggcga atgttgaaca tgggatactc ggtatggagc
     4201 attccggagt ctaaagttta ccacgtagga ggaggaacac ttccaaacaa caacccatac
     4261 aagctctacc tgaactaccg taatagcctg ttcatgctac acaagaacct atcaaagaat
     4321 gccttttacc aaaccattat agccaggcta cttctcgatt ggctttcggc aatggcctac
     4381 ctggttcgct ttcagcttaa atttttcggg gccgttgcta agtcacacta ccattactat
     4441 cgtgatatca gcaggttgaa tagcttccgc agctcattcc ggcatcctaa aaccaacagt
     4501 gcaaaagtta tctaccatag aagcgttctt tttgattttt tcatactccg caggcggaaa
     4561 ttttccgagc tgaagtttta gtcttttgtc agccttttta ccttttttag gataggggcg
     4621 tatgttaaaa atatgaaaaa aatcgccttt tttgataatc taatccttta aaagatacct
     4681 ttgcagctca actttacaca ttagccattt agctaaactg cagcagtacc aaatgatctc
     4741 atttaaaaaa cttaccattc gtgatatttt attccgttca cgaatggcct acgccgaaag
     4801 accagctctt gcttttgttg actcaaagcc tg
//
LOCUS       DAOPHX010000163         4825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_23599_length_4825_cov_6.269811, whole genome shotgun sequence.
ACCESSION   DAOPHX010000163 DAOPHX010000000
VERSION     DAOPHX010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23599_length_4825_cov_6.269811"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..1668
                     /locus_tag="PK502_09835"
     CDS             28..1668
                     /locus_tag="PK502_09835"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPH01159.1"
                     /translation="MCRLFSGRVNNLLFSIFIVLCTLLLFINVGTYGVVESSDARYAE
                     ISREMLESGDYIHPTLLNIQHYHKPPLTYQLTALGYQIFGVNELGARFFLQLSLLIQL
                     LLVYALSNILFGNRGTALWAAIIYFSFPLTLISTRNLTTDSFLATFVLLSIYSWVKYR
                     KYGKFRYLYLTAISLALGFCTKGPVVLLVPLVFIIAYNRIEKSKNRFGVHHLLSLVVF
                     LGISLWWFVFLIRENGAFADYFLGRQTVERFGKDVFNRSEPFWYFVAFAPLVGLPWLL
                     ILPYLMVQNRKSILKKGINSVLTLSFVIPLVFFSISTSKRILYILPFYSILAMLTANL
                     LANLISSKEKVIDRSVTIFSMIVLLVATATMFIHTNYVIPKGVGIASILLIVVILAIY
                     RYLQGSRKFKPVLVSFLVSGFLLITSGLLMQKNELEFNISRPISNFIKSKNLQDRNIL
                     IYNTMKPSIAFDMRKPVISLYDGSNTLAREVQFEENSAWKSYFYDLTDSIDTNRLKGL
                     MERPSVLVSYKHPLPENRQWLAKRFSTRKDIGKWHIYY"
     gene            complement(1831..2205)
                     /locus_tag="PK502_09840"
     CDS             complement(1831..2205)
                     /locus_tag="PK502_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPH01160.1"
                     /translation="MKVAVPTRNGVVDEHFGHCEAYTVFTIDSGNKIENSEILPSPQG
                     CGCHSNIAATLKEKGVEVMLAGNMGDGALNMLAGHGIQVFRGCSGDARRLVENFLSGN
                     VEDSGEGCHHHHEHEEGHTCNH"
     gene            complement(2363..4462)
                     /locus_tag="PK502_09845"
     CDS             complement(2363..4462)
                     /locus_tag="PK502_09845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPH01161.1"
                     /translation="MATLEKIRNRAGVLVAVVIGLALLAFILGDLLSSGGSLFNRAQM
                     EVAKIGGTSIPFEMLQAKIDESENLQKMFSNQLSLDEQTVLRIREQVWQDLVRTYTME
                     DQYKKLGLSIHPDELFDMVQGRNIHPIIQQQFSDPNTGQLNKEFITMFLKNMDQDPRS
                     KTYWLFLEQEIQKDRLFNKYFNLITKGLYVTSVQAQRALEDRTKKVNFDYTLVAYSSI
                     PDSSISVEGKEIKNYYKSHLSEYKQTASRDIEYVVFPIQPSESDRQAAEEWINKMKDE
                     FLLADDPNQYVTLNSDSPLDSKYYKESELTDEMGKWAFSAKVGESYGPTFDGNSYKII
                     RLVNVKMLPDSVKARHILISPKAQNQEAVAKAKATADSLLNVIKRGGSWDDLASRFSD
                     DPGSKDKGGDLGWFPGGVMVKNFDEAAFNLPKNEVKVVETNYGFHILQVTDRGKESRK
                     VQLATLERKVVPSSTTSQRIYSEASQFASSNRTYEQFKTAADAKKLTRRVASNLLAND
                     QNIAGLESPREVIRWAFKAEEGDVSNVFELANMYVVASLTAARSEGVAPLKQVVADVR
                     TKVIRDKKAEKLAARVSDALKGASDIFSVAGKLNGKVEHAENVTFSSFTLPYAGFEPA
                     VIGVASVSSEGKLQGPVNGNAGVYVLAVTSIVTDQGDAGMEKLRLANTYQSRAYYEAF
                     EALKSKIGIVDKRYNFY"
BASE COUNT         1287 a         1142 c          997 g         1399 t
ORIGIN      
        1 taatcgttct ttgtcattct tacaattttg tgtagactct tttcaggacg ggtcaataac
       61 ctgctatttt cgatttttat tgttctatgc accttgcttc tgttcatcaa cgttggtacc
      121 tacggggttg ttgaaagtag cgatgcccgc tacgctgaaa tttcgcgtga gatgctagag
      181 tcgggcgact acattcatcc cacccttttg aatattcaac actaccataa gcccccgtta
      241 acctaccagc ttaccgcttt aggttaccaa atttttggcg taaacgagct tggcgcccgt
      301 tttttcctgc agctctccct ccttattcag ctgcttttag tgtatgccct atccaacatt
      361 ctctttggca accgtggtac cgcattatgg gcagctataa tatacttcag cttcccatta
      421 actctcatat ctacacgaaa tctaacaacc gattcgtttt tagcaacttt cgttctgttg
      481 tcaatatact cctgggttaa gtatcgaaaa tacggcaaat ttcggtacct ctacttaact
      541 gcaattagcc tggctttggg attctgtact aaaggtccgg tggtacttct ggtaccattg
      601 gtatttatta tagcctacaa ccgcattgaa aaaagtaaaa atcggtttgg agttcaccat
      661 ctactttccc tggtggtttt tctgggtatt tcgctatggt ggtttgtttt cctgattaga
      721 gagaacggag cgtttgccga ctacttttta ggcagacaaa cggtggaacg gtttggaaag
      781 gatgttttca accgttcgga gccattctgg tacttcgtag cttttgcacc gcttgtaggt
      841 cttccatggc tactcattct accctactta atggtgcaga acagaaaaag catcctgaag
      901 aagggtataa attctgtgct tacgctaagc ttcgtaattc cactggtgtt cttttcaata
      961 tctacctcaa aaagaattct gtacatactg ccgttctaca gcattttggc aatgctcacc
     1021 gccaacctgc tggcaaatct aattagctca aaagagaagg ttatcgaccg aagcgtaacg
     1081 atattctcaa tgattgtcct acttgttgca acggcaacca tgtttataca caccaattac
     1141 gttataccaa aaggagtagg cattgccagc attctactaa ttgtagtcat tttggcaatt
     1201 tatcgttact tacaggggag tagaaaattc aaacctgtgc tcgtctcgtt tttggtttcc
     1261 gggtttcttc ttataacaag cggcctgctg atgcaaaaaa atgagttgga attcaacatt
     1321 tccaggccaa tctccaactt tattaaaagc aaaaatctac aggacaggaa tatactcatc
     1381 tacaacacca tgaaaccctc cattgctttc gacatgcgaa agccggtgat ttcgctatat
     1441 gatggttcga acactcttgc ccgggaggtg cagtttgagg aaaattcggc atggaaaagc
     1501 tacttttacg acctcactga cagcattgat accaatcggc ttaaaggcct gatggaaagg
     1561 ccgtcagttc tggtttccta caagcatccc ctacctgaaa acaggcaatg gctggcgaaa
     1621 aggttcagca caagaaaaga cataggaaaa tggcacatct actactaacc ctgaaagggg
     1681 gtgaaggcaa ataaaattat aaaggcggtt tcggtgcaga accggattta tcgccacaaa
     1741 aaattaccac tggtaatgtt tctcaacgat tattcatagc ccaggtaaaa aagcctcact
     1801 taatgtagct ccgaatagcc aatatacttg ctaatgattg catgtatgac cctcttcatg
     1861 ttcgtggtga tgatgacacc cctcgcctga atcctcaaca tttccgctca ggaaattttc
     1921 aaccaaccgg cgagcatcgc ccgaacagcc gcgaaatacc tgtattccat gaccagctaa
     1981 catgttcaat gcaccatcgc ccatgttccc tgcgagcata acttctacac ctttttcctt
     2041 cagagtggca gcaatattgc tatggcatcc gcacccctga ggcgatggaa gaatttctga
     2101 gttttcaatc ttattacccg agtcaatcgt gaatacggtg taagcctcac aatggccaaa
     2161 gtgttcgtca acaacaccgt ttcttgttgg tacagcaacc ttcataacat ttcattttat
     2221 ttggttaata attccgataa ttttcgtttt gcctgttcaa ttataaccat tttgcaaact
     2281 tttagttcga aaaaacagtg atgcaaaaaa ggggaaattt tatttcccct cagtagtgaa
     2341 caactttcag gttcgatgct gtctagtaga agttgtagcg cttatcaaca atgccaatct
     2401 ttgatttcaa cgcctcaaaa gcctcgtagt atgccctgga ctggtaggtg ttggccaatc
     2461 ttaacttttc cattccggca tctccctgat cggtaactat agatgtaaca gccaaaacgt
     2521 aaaccccggc gttaccgttg acaggtccct gaagcttacc ctcggagcta accgaagcca
     2581 ctccaataac agctggctca aaacctgcat agggcaaagt aaacgagcta aatgtaacat
     2641 tttcggcgtg ctctaccttg ccgttaagct tacccgcaac ggaaaaaata tcgctggcgc
     2701 cctttaaagc atcactcaca cgtgctgcca acttttcggc cttcttatcc ctgattacct
     2761 tggttcttac atcggcaaca acctgcttta agggtgcaac tccctcacta cgggcagcgg
     2821 taagagaggc aaccacatac atgttggcca actcaaacac gtttgaaaca tcgccctctt
     2881 cagccttaaa tgcccagcga ataacttcac ggggtgattc cagcccggca atattttggt
     2941 cgttggccag caggttggag gcaacccgtc gagtaagttt tttggcatca gcagcggttt
     3001 taaactgctc gtaggttctg ttagatgagg caaattgcga agcttcgctg tatatcctct
     3061 ggctggtggt gctgcttggt acaacctttc gctccagagt agccagctgc accttcctgg
     3121 attccttccc cctatcggtt acctgaagaa tatgaaatcc gtaattggtt tcaactacct
     3181 taacctcatt ttttgggagg ttgaatgcag cctcatcaaa atttttcacc atgacaccgc
     3241 cgggaaacca ccctaggtcg ccacccttat ccttagatcc tgggtcatcg gaaaaacgtg
     3301 aggcaagatc atcccagctc cctccacgtt taattacatt cagcaggcta tctgctgttg
     3361 ccttggcttt agcaactgcc tcctgatttt gagccttagg gctaatcagt atatgacgcg
     3421 ccttcaccga gtcaggtagc atcttgacat tcaccagccg gataatttta taggaatttc
     3481 cgtcaaatgt agggccgtag ctttcgccta ccttggcaga aaaagcccat tttcccatct
     3541 catcagtcag ctcactttcc ttgtagtact tcgagtcgag tggactatcg gagttgaggg
     3601 taacatactg gtttggatcg tcagcgagta gaaactcatc cttcatctta ttaatccact
     3661 cctcagcagc ctgcctatcg ctttcggatg gttgaatggg gaaaactaca tattcgatat
     3721 cccttgaggc ggtctgcttg tactcgctta ggtgagattt gtagtaattt tttatttctt
     3781 ttccctcaac ggaaatagag gaatcgggaa tagaagagta ggccacgagg gtatagtcga
     3841 aatttacctt tttagttcta tcctccaatg cgcgttgggc ttgaaccgag gtaacatata
     3901 agcccttagt aataaggttg aagtacttat tgaaaagcct atccttctga atttcctgct
     3961 caagaaaaag ccagtatgtt ttggagcggg gatcctggtc catgttttta agaaacatgg
     4021 tgataaactc cttgttcagc tggccggtat ttggatcgct aaactgctgc tggattattg
     4081 gatggatgtt ccttccctga accatatcaa aaagctcgtc agggtgaatg gagagcccca
     4141 gctttttgta ctggtcttcc atggtgtatg ttctaaccaa atcctgccaa acttgctccc
     4201 tgatacgcag caccgtttgc tcatcgagtg atagctggtt ggaaaacatc ttctgaagat
     4261 tctcgctctc gtcaatcttt gcctgcagca tttcgaaagg tatagaagtg ccaccaattt
     4321 tagcgacctc catttgagca cggttaaata gcgatccgcc tgaacttaac aggtcgccca
     4381 aaatgaatgc cagcaatgct aaaccaatca ccaccgcaac aagaacacct gcccggtttc
     4441 taattttttc gagtgtagcc atttaaatga tatttaaatt atagaaattc agctaaaatt
     4501 caagccacga atatagcaat tttagcaata gggctttaag caccaagcaa tttttttata
     4561 tgggggttaa ctaaaaggga gagagaaagt gagtcaagat gttcaaggat aaaggataga
     4621 atcgtaaatc gtttgccttt taccaaaccg ggaaaagtta accttatcct ttacccttca
     4681 actttctcca tgtcttcagc cccagcgggg gaaaggtatt ctcagcggtg cacggaaagg
     4741 cgggaatcgt tgaacgttac tggtataagt aaatggcata aaagcctgac ccgcaaatgg
     4801 gtcgggggcg gaacgtagcg cggga
//
LOCUS       DAOPHX010000164         4800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_23772_length_4800_cov_6.263857, whole genome shotgun sequence.
ACCESSION   DAOPHX010000164 DAOPHX010000000
VERSION     DAOPHX010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23772_length_4800_cov_6.263857"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2498
                     /locus_tag="PK502_09850"
     CDS             <1..2498
                     /locus_tag="PK502_09850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPH01162.1"
                     /translation="NIFINLPANDYSIVVNDVNGCLQSTLRTINQPAQITIDNVVVVQ
                     ESTPGANDGEIHVTATGGTGALTYTLQPNGISNGTGNFTGLGTGTYHVDVMDANGCMV
                     QTGDLIISGVNINITSNNVSCHGGSDGLITITITGGFPPFTVVCNEIDTTPIPVNDLG
                     GGVFSAEGLSAGSYLITVTDNVPHTFPTYQIDILEPDSLIATFVSLQNPQCHGGADGN
                     VLFNITGGTPVYTITWDGGTSVGTTATPVTAGYHEFTITDQHGCSTVVSDVPELVEPN
                     PIQLVEQTQFDPTCHGEAIGIISVTATGGTGTLTYNLSGTATLSNTTGVFENLFAGVY
                     DLSVTDDNGCPDNFDPPVRFTLNEPPQIIISGLTPDDSLICPNDPSGFVRVQVSGGQP
                     DYTYLWSNGQTTVDLDSVVVGDYTITVTDALNCRVSQTFTVRGTPMLELSPVVIDTAL
                     CRIKPTGNTGRIDIRNSTGGNGQFADLEFQWNDANATTGPTLPKAGDPMIIGGGIYTV
                     TVKDTSGCVYSFDYEVPINPRYNFLTNAGSDTTVCYSNPVILHGSVISGNEGGTVDTT
                     FDFSYEWRKFPDIDGDPIHRGQDYQVNLVESSSFLLWAIENNSGCKDSTRINLNVLPK
                     IGLEVPLYISAVQDTIISILMGKEVNLDVITRSVEYATEFQWKPAEMFNPPDSWNSSI
                     LINDQIKQQIPEERKQKLTDPISKKPTDFILVDVIATTEMGCTDSLRLYTKVVESLGF
                     ANVFSPNGDGINDVWGVPKDYLFPDLSIEIFNRWGSLVWSATGDKAAKGWNGRTSNGN
                     ELPIGTYYYIVKFNVQAQGASWKPITGSVTIVK"
     gene            2658..3599
                     /locus_tag="PK502_09855"
     CDS             2658..3599
                     /locus_tag="PK502_09855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPH01163.1"
                     /translation="MKTTRTAAYLVYPLVTVIMLGMAGQVNSQQIPHYSQYMFNNYLT
                     NPAVAGTLNHYQMRLNNRYQWVGLNDAPYTVNMSFYGPFTKHDMGWGGYFSNDVTGPT
                     SRTSIMGTYAYNMQVSQSGMRISGGISFGMMIYKLDGSKLTLGEEPDPMYDPAIIQAT
                     KSVFTPDASVGFYLYSTSFNVGLSAHQLFGQKLKFYPEPIGVNRLKQHIMLSGGYWVN
                     LNRYMELETSAIIKYMFASPMQFELNGKISYRQKEYQFWGGLSLRWKDAVSALVGINW
                     NRKFMFGYSFDWSLLGISKYNSGSHEIMLGYMFDKIK"
     gene            3696..4541
                     /locus_tag="PK502_09860"
     CDS             3696..4541
                     /locus_tag="PK502_09860"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HPH01164.1"
                     /translation="MRKMVIWLVGLPAVVIACLSCGNSGIIPENDMVKILSKIYITDG
                     ITFLPEKHTLFGRDTIAYYEPIIESYGYTTQEFDSSMKYYAYHTEKLDEIFDRVIMEL
                     SKMEEQASINPKNLEEDQNTPTDTSENLWPHKTFWNMAVDYGQNRSLGFDIPVKGLGD
                     YTLSFEAQLFPDDSSVECRMNAFWFIADNVQRDYRDVAKVQLYPKDGVVHHYSFTFSC
                     TDSQYTHLKGWLYDNANKNQNFNRHAVFNNIKVTYKPAPKSPVRNLKDKKPIKAFRKP
                     IPVQQ"
     gene            4543..>4800
                     /locus_tag="PK502_09865"
     CDS             4543..>4800
                     /locus_tag="PK502_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HPH01165.1"
                     /translation="MHRFSANYIFPVSQPPIRNGVVEVDDDGVIMRVFHPGNSPKEIH
                     STEFYNGVIVPGFVNAHCHLELSHLRGKIPKNLGIAGFIKAI"
BASE COUNT         1340 a         1088 c         1081 g         1291 t
ORIGIN      
        1 ccaacatttt catcaacctg ccggcaaacg actacagcat cgtggtaaac gatgtgaatg
       61 gctgcctgca atcaaccctt agaaccatta accaacccgc tcagattacc attgacaatg
      121 ttgtggtggt gcaggaaagc acaccaggtg caaacgatgg cgaaatccat gttactgcta
      181 ccgggggaac cggagcctta acatacaccc tgcaacccaa tggaatttca aatggaacag
      241 gcaattttac tggcttaggc actggaacat accatgttga tgtaatggat gctaatggct
      301 gtatggttca aacaggcgac ctcatcatta gcggagttaa tatcaacata acctctaaca
      361 atgtaagctg ccatggaggt tccgatgggc ttatcaccat taccattacc ggaggctttc
      421 caccctttac cgtagtttgc aatgaaattg acacgacacc gattcctgtt aacgatctag
      481 gcggtggagt attttcagct gaggggttat cggcaggttc ctacctcata accgttactg
      541 ataatgttcc gcataccttc cccacctatc aaattgatat actagagccc gattcgctta
      601 ttgccacatt cgtcagctta caaaatcctc aatgccacgg tggagctgat ggtaacgttc
      661 tgtttaacat tacaggcgga actcctgttt atacgattac ctgggatggt ggaacttcgg
      721 tgggtacaac agctaccccg gttacagccg ggtaccacga atttaccata accgaccagc
      781 atggttgttc aactgttgtt tcggatgtac ccgagctggt tgagccaaat ccaattcagc
      841 tggtagagca aacgcagttt gatcccacct gccatggcga agcaataggt attattagcg
      901 ttacggctac cggtggcacg ggcacattaa cctacaacct ttccggaaca gctacccttt
      961 cgaatactac cggagttttt gaaaacctgt ttgccggcgt ttatgacctt agcgttacgg
     1021 atgacaacgg gtgtcccgac aacttcgacc cacctgttag gtttaccctg aacgaaccgc
     1081 cacaaattat tatcagtggc ctaacaccgg atgattcgct gatttgtccg aacgatccat
     1141 cgggctttgt tagagttcag gttagcggag gtcaacccga ttatacctac ctctggtcga
     1201 atgggcaaac tacggttgat cttgatagcg tagttgttgg agactacacc ataaccgtta
     1261 ctgatgccct gaactgtagg gtttcacaaa ccttcacagt cagaggaaca ccaatgcttg
     1321 aactttctcc ggttgttatt gatacggcac tatgtagaat aaaaccaacc ggtaataccg
     1381 gaagaataga catccggaat tccacaggag gtaatggcca gtttgctgac ctggaatttc
     1441 agtggaatga tgctaacgca accaccggtc ctactcttcc caaggcggga gacccaatga
     1501 ttattggtgg aggcatatac acagttacgg ttaaggatac cagcggctgt gtgtattcct
     1561 tcgattacga ggtacccata aatccccgtt acaatttcct tactaatgct gggagcgata
     1621 ccaccgtttg ctatagtaac cctgtaattc ttcatggttc ggttatatct ggaaatgaag
     1681 ggggaacggt agataccacc ttcgacttct cctacgagtg gcgtaaattc cccgatattg
     1741 atggcgatcc aattcatcgg ggtcaggatt accaggttaa ccttgttgaa agttcatcat
     1801 tcttgctttg ggctatagaa aataactctg ggtgtaagga ctctacccga ataaacctca
     1861 acgtgctccc caaaattggg cttgaggttc ccctttacat ttcggctgtt caggatacca
     1921 tcatctcaat tcttatgggt aaggaggtta accttgacgt tattacacgc agcgtagagt
     1981 acgccacgga gtttcaatgg aaacctgccg aaatgttcaa tccgccggac agctggaata
     2041 gctccatact aatcaacgac caaataaagc aacaaatacc tgaagaaagg aaacaaaaac
     2101 ttaccgaccc gatatccaaa aagccaaccg atttcatcct ggttgatgtg attgccacca
     2161 ccgagatggg ttgcaccgat tccctgcggc tttacacaaa ggtggttgaa agtctaggtt
     2221 ttgccaacgt attctcgcca aacggagatg ggataaacga tgtttggggt gttcccaagg
     2281 attacctgtt ccccgattta tccatagaga ttttcaaccg gtgggggtcg ttggtttggt
     2341 cggctaccgg cgataaggcg gccaagggat ggaatggaag aacaagcaat ggcaacgagc
     2401 tgcccattgg aacctactac tacatagtaa agttcaacgt tcaggctcag ggagccagct
     2461 ggaaacccat tacaggttct gttacaattg tgaagtaaag gctgtaatgt ttaggataca
     2521 atttttatat cctatgagtt tatagttaat ggctcaaaag aattaaagga attgtacaaa
     2581 ggcattttag gatagggttt aaattaaaaa tattttttgt agcagctatc aaagtaccgg
     2641 tatataagtt taaacgcatg aaaacaacac gtacagcagc ttaccttgtt tacccgctag
     2701 ttactgttat tatgctggga atggcgggtc aggtaaattc gcagcaaata ccccattact
     2761 cccagtatat gttcaataac tacctgacca acccggcagt ggctgggaca ttaaaccact
     2821 accagatgag gttgaacaac cggtatcaat gggttgggct caacgatgca ccctacacgg
     2881 ttaacatgag tttttacggt ccatttacca agcacgatat gggctggggt ggttactttt
     2941 cgaatgatgt taccgggcct acaagtcgga caagcataat gggaacctat gcctacaaca
     3001 tgcaggtaag ccagtcggga atgagaattt ccggtggaat ttcattcggc atgatgattt
     3061 ataagctcga cggctcaaag ctcaccctgg gagaagagcc agatccgatg tatgatccgg
     3121 ccattattca agctacaaaa tctgttttta cacccgatgc ctctgtagga ttttaccttt
     3181 acagcacctc atttaacgtt ggtctgtcgg cacaccagct ttttggtcaa aagttgaagt
     3241 tttaccccga gcccatcggt gttaaccgcc taaagcagca tatcatgctt agcgggggtt
     3301 actgggtaaa cctcaacagg tacatggagc tggaaacatc ggccataatc aagtacatgt
     3361 ttgccagtcc catgcagttt gaactaaacg ggaaaatcag ctaccggcag aaagaatacc
     3421 aattttgggg aggcctttcg cttcgctgga aggacgccgt atcagccctt gttggcatta
     3481 actggaacag gaagtttatg ttcggttact cgttcgactg gagccttctg ggcattagca
     3541 agtacaactc cggatctcac gaaattatgc ttggctacat gtttgataaa attaagtagc
     3601 gggaatgcat gtaggatatg tttgccggac accgttgtcc ggttttttca ttttcaccta
     3661 aattgaagta gatttgcagc tgtaaattat gtagaatgag gaaaatggta atttggctgg
     3721 taggattgcc ggctgtggtt atagcttgcc tgtcatgtgg aaattccgga attattcctg
     3781 agaatgacat ggttaaaatc ctttcaaaga tttacattac cgatggcata acttttcttc
     3841 ccgaaaagca tactcttttt ggtagggaca ccattgccta ctacgagccc atcatcgaat
     3901 cgtatggcta tacaacacaa gagtttgaca gctcaatgaa gtactacgcc taccacaccg
     3961 aaaagctgga tgagatattt gacagggtta ttatggagct atccaaaatg gaagaacaag
     4021 ctagtatcaa ccctaaaaat ttagaggaag accaaaacac ccctacggat acttctgaaa
     4081 atctttggcc tcataaaaca ttctggaaca tggctgttga ttatggtcaa aaccgctccc
     4141 taggcttcga tattcctgtt aaagggcttg gtgattacac tctttctttt gaagcccagc
     4201 tgtttcccga cgactcttct gttgagtgcc gcatgaatgc gttttggttt atagccgata
     4261 atgttcaacg ggattatcgt gatgtggcta aagtccagct ctaccctaag gatggagtgg
     4321 ttcaccacta ttcctttacg tttagctgca ccgatagcca gtacacgcat cttaaaggct
     4381 ggctctatga taatgccaat aagaatcaaa actttaaccg gcatgcagtt ttcaacaata
     4441 taaaggttac ctacaagccc gcaccaaaga gcccggtgcg taatttaaag gataaaaaac
     4501 ctataaaggc ctttcgaaag cccattccgg ttcaacagta acatgcacag gttttctgca
     4561 aactatattt tccccgtcag ccagccacca attcgaaacg gagttgttga ggtggatgac
     4621 gatggtgtta ttatgagagt ttttcatcct ggaaattctc caaaggaaat tcattccacg
     4681 gagttttaca acggggtaat tgttccgggt tttgtaaatg cccactgcca tttggaactc
     4741 tcgcatcttc gtggtaagat acccaagaac ttgggcattg cgggttttat taaggccata
//
LOCUS       DAOPHX010000165         4668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_24707_length_4668_cov_5.196185, whole genome shotgun sequence.
ACCESSION   DAOPHX010000165 DAOPHX010000000
VERSION     DAOPHX010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24707_length_4668_cov_5.196185"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PK502_09870"
     CDS             <1..741
                     /locus_tag="PK502_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPH01166.1"
                     /translation="ILSRMFIHSESYSDALCQAQSLGFAESNPDFDVMGFDALYKLII
                     LALHSFGTFIQPEQAFCYGIQNLTNFDIGYAREKGKKIKLVAHLRKFSESCFTLFVIP
                     TLVAPDDYIFNVENEFNGVVIEGQYYDKQFMFGKGAGGFPTASAVLSDITARLHGYRY
                     EYKKFYFSSPPSYTTDVVLSLYIRYSRESDLNVFDILEVQERYNGPEYRWVVARVKLS
                     SLLKIKELLPKRNLFTIFTGEYSGGILS"
     gene            772..4443
                     /gene="metH"
                     /locus_tag="PK502_09875"
     CDS             772..4443
                     /gene="metH"
                     /locus_tag="PK502_09875"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HPH01167.1"
                     /translation="MKSKELIAEQLNKRILVLDGAMGTMIQQLGLTEEDYRGAVFANH
                     PVNLKGNNDVLALSRPDVIASLHKQYLEAGADIIETNTFNANAVSQADYALEDWIEQI
                     NLEAAGIARRLADDFTTLRPDKPRFVAGSIGPTNRTASMSPDVNRPGYRAVTFDTLVQ
                     AYSLQIKALIKGGVDLLLVETVFDTLNAKAALFAAASVMEELQTDIPVMISATIADLS
                     GRLLTGQTLEAFVTSVSHYPLLSIGLNCAFGAEQLLPYVKQLAVVTPFRVSVHPNAGL
                     PNQLGGYDQSAEMMAGWVEQMVQAGAVNIVGGCCGTTPKHIERVAAVASKYPPRKTPT
                     LQRFTRLSGLEMLEIRPESNFINIGERTNVAGSKKFEKLIIEEKYGEALAIAREQVEG
                     GAQAIDVCMDGALINAPVAITEFLNLVASEPDIARVPVMIDSSRFEVIEAGLKCTQGK
                     SIVNSISLKEGEQEFIRRAKLIRRYGAAVVVMLFDEEGQATTTEHRCRVAMRSYNLLV
                     QTVGFAPEDIIIDPNILAIGTGMAEHSSQAVSFIETTKWIKQNLPYAGVSGGVSNLSF
                     AFRGNNQIREAIHTVFLYHAVNAGMNMGIVNPGMLRIYTDIEPDLLRLTEDLVLNRRH
                     DATERLLAYAHNVKDDKTDDKALPEWRSYPPGKRLEHAIIKGNEDYVEVDTLEAFSIM
                     GSAISVIEGPLMDGMKEVGTLFGSGRMFLPQVVKSARVMKKAVGILEPYISSSDESSK
                     IQKSKGTIVLATVKGDVHDIGKNIVSVVLACMGYEVVDLGVMVPTDKIINGVIGHKAD
                     ILGLSGLITPSLEEMANVARELEHSGLTIPLLVGGATTSELHTALKIDTEYSGSVIHV
                     KDASLAGGVVAELLSPNQHEMYTQKVKERYAGLRSTYSEQKKALISIDDARQNRFKFD
                     WSSYTPPQPNHRGIQPFLNVDISELRPYISWTFFFHAWGLKGRFPEILTDPVTGSEAK
                     KLLDEANRMLDDIAAAKSIRASGVAGLFPAASQNDDILVFTDETRSSVMATLPQLRNQ
                     QLKTNGEFNLSLADYLAPLNSGIPDWLGCFVVTSGIGVDELANKYRAAGDDYKAIMVK
                     LLADRLAEAFAEKLHQQVRKELWGYRPNENLSPAEVLAGRYDGIRPAPGYPACPDHRG
                     KKFIFDLLKASDLLGVELTETLVMQPLASVCGYYFSHPKSSYFNIGRVTNDQLGDYAR
                     RLGVSLEEAVRFFPTNVEQ"
BASE COUNT         1240 a         1042 c         1160 g         1226 t
ORIGIN      
        1 atcttgtcaa ggatgtttat ccattctgag tcatactccg atgccctttg ccaagctcaa
       61 tcgctcggat ttgccgaaag caaccccgac ttcgatgtga tgggttttga tgcgctctat
      121 aagcttatca tactagcttt acatagcttt ggaacattta ttcaaccgga acaggccttt
      181 tgttacggaa tccaaaattt aaccaatttc gacattgggt atgcccggga aaagggcaaa
      241 aagataaagt tggttgccca tttgcgaaaa ttttccgaaa gctgcttcac gctttttgta
      301 atccctactc tggttgctcc cgacgactac attttcaatg ttgaaaatga gtttaacggg
      361 gttgtcattg agggccaata ctacgataag cagttcatgt ttggcaaagg agccggaggt
      421 ttcccaacag cctcggctgt cctgagcgac attactgcca ggctgcacgg ttaccgttat
      481 gagtacaaaa agttttactt ttcatctcct cccagctata ctaccgatgt agttcttagc
      541 ctttacattc ggtatagccg ggagtcggac ctcaacgtat tcgatattct ggaggtgcag
      601 gaacggtata acggacccga ataccgctgg gtggtggcca gggttaagct atcgtcactt
      661 ctgaaaatta aagaattact ccccaagcgg aatctgttta ctatctttac cggtgaatac
      721 agcggtggaa ttctctctta aaaccccaca aacgtaaagg aaagacaatt aatgaaatcg
      781 aaggaattga tagctgagca gcttaacaaa aggatactgg tgcttgacgg ggccatggga
      841 accatgattc agcagcttgg ccttacggag gaggattacc gtggcgcagt gtttgccaac
      901 catcctgtca acctgaaggg taataatgat gtgcttgcgc tttctcgtcc cgatgttatt
      961 gcatcgcttc ataagcagta tcttgaagca ggtgctgata tcatagaaac aaacactttc
     1021 aacgcaaacg cagtttcgca agccgattac gcattggagg attggattga gcagataaat
     1081 ttggaggcag ccggtattgc acgacgacta gccgacgatt ttacaacact tagacctgat
     1141 aaacccaggt ttgtagcggg ctcaattggc cctactaacc gaacagcatc catgtcgccc
     1201 gatgttaacc ggcctggtta ccgtgcggtt acatttgata ctttggttca ggcatacagc
     1261 ttgcaaatca aagcgttgat aaaaggtgga gtggatttgc tgctagttga aacggttttc
     1321 gatacgctta acgccaaggc agcactgttt gccgctgcca gtgtcatgga agaactgcaa
     1381 accgacatcc cggtgatgat ttcagcaaca atagccgatt tgagcggacg tttgctaacc
     1441 ggccaaaccc tggaagcttt tgttacctcg gtttcacact atccgctttt gagcatcggg
     1501 cttaactgtg cttttggtgc cgagcagctg ctaccctatg tgaagcagct tgctgtggta
     1561 acgccattta gagttagcgt tcaccctaac gccgggcttc caaaccagct tggaggttac
     1621 gaccaatctg ccgagatgat ggccggttgg gtggagcaaa tggtacaggc aggagcggtg
     1681 aacattgtgg gcggatgctg tggtacaacg ccaaaacaca tcgagcgggt tgccgccgtt
     1741 gcttccaaat acccacctcg aaaaacacct acattacagc gcttcacccg cttaagcgga
     1801 ctggaaatgc tcgaaattcg acccgaaagt aattttataa atattggtga acgcactaac
     1861 gtagccggct ctaaaaaatt cgagaagcta attatcgagg agaagtacgg tgaagcgtta
     1921 gccattgctc gtgaacaggt tgagggtggc gcgcaggcca tcgacgtgtg tatggatggc
     1981 gccctgatta atgcccctgt tgcaattacc gagtttctta acctggttgc atccgaaccg
     2041 gatattgcaa gagttccggt tatgattgat tcatcccgct tcgaggttat tgaagcgggc
     2101 ctcaagtgca cacagggcaa aagcattgtg aattcaataa gcctgaagga gggtgagcag
     2161 gagttcatcc ggagggctaa gcttatcagg cggtatggtg ctgcagttgt tgtcatgctt
     2221 tttgatgagg aagggcaggc aacaaccacc gaacatcgct gcagggttgc catgcgctcc
     2281 tacaaccttc tggttcaaac agtgggcttt gcccccgagg atataattat tgaccccaac
     2341 attctggcta ttggaaccgg aatggccgag cactcatcgc aggcggtcag ctttattgaa
     2401 accaccaaat ggattaagca aaacctaccc tatgcaggag ttagcggggg tgtgagcaac
     2461 ctttcgtttg catttcgtgg aaacaaccag attcgggagg cgatacatac ggttttcctt
     2521 taccatgctg taaatgccgg tatgaacatg ggaattgtga accctggcat gctccgcatt
     2581 tacaccgata ttgaaccaga tttgcttagg ttgacggagg atttggtgct taaccgtcgc
     2641 catgatgcaa ccgaacggct actagcctat gcccataatg taaaggatga taagacagat
     2701 gataaagctt tgccggaatg gaggagttac ccacccggta aacgccttga gcacgccatt
     2761 ataaagggaa atgaggatta cgttgaagtt gatacgcttg aggcctttag cataatgggg
     2821 tctgcaatat ccgttattga agggccttta atggatggca tgaaggaagt tggcaccctt
     2881 tttggctcag gtagaatgtt tcttccgcag gtggtgaaga gcgcaagggt tatgaagaaa
     2941 gctgtcggta ttctcgaacc atatataagt agttcggatg aaagtagcaa aattcaaaag
     3001 tcaaagggaa caatcgttct tgccaccgtt aagggtgatg tccatgatat tggtaaaaat
     3061 attgtgagtg tggtgctggc ctgcatgggg tatgaggtgg ttgacctggg cgtgatggtt
     3121 ccgactgata aaattattaa tggggtaatt ggccataaag ccgatatttt ggggcttagc
     3181 gggcttataa ctccatcact tgaggaaatg gcaaatgtgg ccagagagct ggagcatagt
     3241 ggattaacta tccccttact tgttggtggg gcaacaacat ccgaactaca cactgccctg
     3301 aaaattgata ccgagtattc aggttcggtt attcacgtaa aggacgcttc gctagccggt
     3361 ggcgtggttg ctgagttgtt atctccgaat caacatgaga tgtacactca aaaagttaag
     3421 gaacgttacg ccggactccg aagcacttat agcgagcaga aaaaagcact gatttctatt
     3481 gacgatgccc gtcaaaacag gtttaagttc gattggagca gctacacacc gccacaaccc
     3541 aaccataggg gaatccaacc attcctaaat gttgatattt cggagttacg accctacatt
     3601 agctggacat tcttttttca cgcctggggg ctcaagggac gatttcccga aattcttacc
     3661 gatcctgtta caggttccga ggctaaaaag cttctcgatg aggctaaccg aatgctggat
     3721 gacatagccg cagcaaaatc aattcgagcg tcaggtgtag cagggctttt ccctgctgcc
     3781 tcgcagaacg acgatatttt ggtatttacc gacgaaaccc gaagttcggt gatggctacc
     3841 ctgccacagc ttagaaatca gcagctaaag acaaatgggg agttcaacct ttcactggct
     3901 gactatctgg caccgctaaa ctcgggcata cccgactggc taggttgctt tgtggtaact
     3961 tcaggaattg gggtagatga gctggccaac aagtaccgtg ctgcaggcga cgattacaaa
     4021 gctatcatgg ttaaactgtt ggccgacagg ctggccgaag cttttgccga aaagctacat
     4081 cagcaggtac gaaaggagct ttggggttat agacctaacg aaaacctctc acctgccgaa
     4141 gttttagctg gtaggtatga tggtattcgt ccggctcctg gttatcctgc ctgccccgat
     4201 caccggggga aaaagtttat ttttgatctt cttaaagcat cagatttact tggagttgag
     4261 ctaaccgaaa ccctggttat gcaacccctt gcgtcggttt gcggttacta cttctcgcat
     4321 cctaaaagca gctacttcaa tattggcagg gttaccaacg accagcttgg cgattatgcc
     4381 cgtaggttag gcgtaagcct tgaggaagct gtaaggtttt tcccaacaaa cgtcgaacag
     4441 tgaatttttt aaaacggtat agatattcct gcaagttaga gttttatttt tttaacttta
     4501 gtgcagaatg ttgaaatccg ggttgatatt ttcccgtcac acttcaacaa tccatcaata
     4561 tgaaggtaat agacattcta acaaaatcgc gtagcacctt attctccttt gagctgctgc
     4621 ctcccttaaa gggtcatacc atagataccg tttttgacac aattgaca
//
LOCUS       DAOPHX010000166         4654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_24819_length_4654_cov_5.229180, whole genome shotgun sequence.
ACCESSION   DAOPHX010000166 DAOPHX010000000
VERSION     DAOPHX010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24819_length_4654_cov_5.229180"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(378..1643)
                     /locus_tag="PK502_09880"
     CDS             complement(378..1643)
                     /locus_tag="PK502_09880"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPH01168.1"
                     /translation="MNNLNLLRLGLALLMFCCQLQGSAAPIKHTPKQICPVVEMLNEL
                     EKLNIFETTDSHLVLTDTLRAQPDLVYEYFIAEIGKQSPIDFDYNPHVRRYIDVYTTE
                     RREQVSQMLGLAELYFPLFDEYLDKYHLPLELKYLAAVESALNPLAVSKTGAAGIWQF
                     KINTARMFDLKVSSYVDERMDPDKSTEAACRYLEYLYRTFNSWHLAIAAYNVGPEPIR
                     KAIARNHGETNFWKLYDDLPESAQNYVPAFIAAAYVMQHYGDHGIFPARPTIAFAQTD
                     TVHVAFAVNFSTLSSALNIPVELLRYLNPMYRIDYIPKDSQRQVLRLPSDKAVLFTAN
                     KESILFNSPKTTHQQSITSGSASGSSKKSKMLIYTVKDGDSINKIAINYYCTLDLIRQ
                     WNPGLDTLLNVGQTLTLWVSEDELQKINS"
     gene            complement(1658..1855)
                     /locus_tag="PK502_09885"
     CDS             complement(1658..1855)
                     /locus_tag="PK502_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPH01169.1"
                     /translation="MTASVLALIGGWEWVVIILAILLLFGGKKIPELMRGLGRGVSEF
                     KKGMKDEENGPKEPEKDKKED"
     gene            complement(1878..3197)
                     /gene="rseP"
                     /locus_tag="PK502_09890"
     CDS             complement(1878..3197)
                     /gene="rseP"
                     /locus_tag="PK502_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPH01170.1"
                     /translation="MEILVKVGQFFLSLSILVILHEMGHFMFAKLFKTRVEKFYLFFN
                     PWFSLFRFKHGETEYGIGWLPLGGYVKISGMIDESLDKEQMKQPPQPYEFRSKPAWQR
                     FLIMIAGVMVNVVLAIVIYCATLYTWGEEYLPTRSLKYGVSCDSLAQSIGFQNGDKIL
                     SVSGREVENFQMVAHDILLNDDRTVEVLRGNDTIKITVGEEALASLVKNPLIFIPRIP
                     FIVDEVRPNEPAQAAGFLKGDKLLAVNNTPKQFFDEFRQAMQENKSKTVTFTVERGGQ
                     PVNLEVNVPESGIIGIVPVTDLTKFFEINRKDYTLLQSIPAGIARGTATIKSYLKQLK
                     LIFKPETKAYESLGGFISIGKIFPGTWDWQAFWNLTAFLSIILAIMNILPIPALDGGH
                     VLFLLYEMVSGRKPSDKFLEYAQIIGITLLLLLIVYANANDIVKLFR"
     gene            complement(3197..4354)
                     /locus_tag="PK502_09895"
     CDS             complement(3197..4354)
                     /locus_tag="PK502_09895"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPH01171.1"
                     /translation="MKNVAILGSTGSIGTQALQVIESNPELFSVELLTAQNNWKLLVS
                     QALKYQPNAVVIGNDKYYVNVKSALEKTDIKIFAGKNSIADCVGSAQVDMVLVALVGF
                     AGLEPTISAIKAGKTIALANKETLVVAGELISELAMEHKASVLPVDSEHSAIFQSIVG
                     ERSPIEKIILTASGGPFRNFSLEQLGRVTPSDALQHPNWCMGNKITVDSASLMNKGLE
                     VIEARWLFNLKPSQIEVVVHPQSIVHSMVQFEDGSVKAQLGLPDMRIPIQYALTYPFR
                     VKSDFPRINFGNNLQLTFERPDTVRFPNLALAREALERGGNMPCVLNAANEVAVEGFL
                     EQSIGFMQMSEIIAEVMSKSSYIAYPSLDDYKMTDIETRLKTAELISQNLA"
     gene            complement(4367..>4654)
                     /locus_tag="PK502_09900"
     CDS             complement(4367..>4654)
                     /locus_tag="PK502_09900"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPH01172.1"
                     /translation="VAAPNEVVLAVAKGTVILADWTIETGYTIAVQHENNFISVYKHN
                     SKLLKRQGDVVGAGDAIAIIGNSGELTTGPHLHFELWQNGNTLDPSKFIHF"
BASE COUNT         1311 a         1055 c         1008 g         1280 t
ORIGIN      
        1 tgtattctta cttcggtttt accattcatt ttaacatatt tttattacaa tttccactgc
       61 atggttttga accagggtgc tatattaata aattgttaag aaaaatcata ttgtcctata
      121 accataattg cattactcgt aagccgaaat aaatagcaat attctaatta ccaataattt
      181 aatactgata ctgactattt gtattaaata gtcaaaaata aaaaatattt atacccgtta
      241 agattttgtt ataaaattct taacaagaca aaaatctctg tggctgaaac gcaagaaact
      301 aatactttgc tagcccatag aggagacttg gtgcatatac agctgaaagt gatcaacaag
      361 cgcagccgaa gttagggcta gctgttgatt ttttggagtt cgtcctcgct aacccaaagc
      421 gtgagggttt ggccaacgtt taggagtgtg tcaaggccag gattccattg cctaatcagg
      481 tcaagcgtgc agtagtagtt gatggcaatc ttgtttatac tatccccatc cttaaccgta
      541 tagattagca ttttcgattt ttttgaactt ccacttgctg acccggaagt tatggactgc
      601 tgatgggttg ttttgggtga gttgaaaagt atggattcct tattagctgt aaataaaaca
      661 gctttatcgg atggcagccg taagacctgc cgttggctgt ctttggggat atagtcgatc
      721 cggtacatgg ggttcaggta ccggagaagt tccactggga tgttaagcgc cgaagatagt
      781 gtggagaagt tgacggcaaa ggctacatga acggtatcag tttgggcaaa ggcaattgta
      841 gggcgagcag gaaaaatgcc gtggtcgcca taatgctgca ttacgtatgc ggcagcaata
      901 aaggcaggaa catagttttg agctgattca ggcagatcat cgtaaagctt ccaaaagttg
      961 gtttcaccat gatttcgtgc aatagctttt cttataggtt ctggtccaac attataggct
     1021 gcaatggcta aatgccagct attaaatgtc ctgtaaaggt actccaggta acggcatgct
     1081 gcttcggttg atttatccgg atccatccgc tcatcaacgt aggatgaaac ctttaaatcg
     1141 aacattcgcg ctgtattgat tttgaactgc caaataccgg cagcaccggt tttagaaacg
     1201 gccaggggat taagagccga ttcgacagca gcaaggtatt ttagttctaa gggcaggtgg
     1261 tacttatcga ggtactcatc gaacagggga aagtatagct ccgcaagccc cagcatctgc
     1321 gacacctgtt ccctgcgttc agtagtgtaa acgtcaatgt acctcctcac atgaggattg
     1381 tagtcaaaat caataggcga ttgcttccca atttcggcaa taaagtactc gtaaaccagg
     1441 tcgggttgag cccgcagcgt gtcggtcagg acaagatggg aatcggtagt ctcaaaaata
     1501 tttagctttt cgagctcatt aagcatctcc actacagggc aaatctgttt tggggtatgt
     1561 tttatagggg cagcacttcc ctgtagctga cagcaaaaca tcagtagcgc taagcctaat
     1621 ctgagtaggt ttaggttatt catgaaaata gccggggtta atcttctttt ttatcctttt
     1681 cgggttcttt tggcccattc tcctcatcct tcatgccttt cttaaactcg ctaaccccgc
     1741 gtcctagtcc acgcatgagc tcaggaattt tttttcctcc aaacagcagg agaatagcca
     1801 ggattattac aacccactcc caacccccaa ttagcgctaa aaccgatgct gtcatgttga
     1861 tatgtattta gtgatggtta cctgaaaagc ttcacgatgt cgtttgcatt tgcgtaaaca
     1921 atcaacagga gtaaaagggt tatgccaata atttgggcat actcaagaaa cttatcgcta
     1981 ggtttacgcc cgcttaccat ttcgtaaagt aaaaacaaga catggcctcc gtctaatgca
     2041 ggaatcggca aaatgttcat aatggccaga ataattgata aaaaggccgt taggttccaa
     2101 aaagcctgcc aatcccaggt tcccgggaaa attttcccta ttgagataaa accgccaagt
     2161 gattcgtagg cctttgtctc aggcttaaaa ataagcttaa gctgcttaag gtagctttta
     2221 attgtggctg ttcccctggc aataccggca ggaattgatt gcagaagagt gtaatcctta
     2281 cggttaatct caaaaaattt cgttaaatcg gttacaggaa caataccaat gatacccgat
     2341 tcaggaacat tcacctcaag gttgacgggc tgtccaccac gttcaaccgt aaaggtaacg
     2401 gtttttgatt tattctcctg catagcctga cggaactcgt cgaagaactg cttgggggtg
     2461 ttatttacag ccagcagctt atcgcccttc aaaaatccgg cagcctgtgc aggttcattt
     2521 ggtcttacct catcaacaat aaagggaata cgtggtatga aaatcagggg gttctttacc
     2581 aggctggcca gcgcttcctc cccaactgta atttttatgg tgtcgtttcc acgtaaaacc
     2641 tctaccgttc ggtcgtcgtt gaggagaata tcgtgggcaa ccatctggaa attttcaact
     2701 tctctcccgc ttaccgaaag aattttgtcg ccgttctgaa agccaatgct ttgagcaagg
     2761 gaatcacagc tgacgccgta cttcaggctt cgtgttggaa gatactcctc gccccaggta
     2821 tagagcgtag cgcagtaaat cacaatggcc agaactacgt tcaccattac tcctgcgatc
     2881 attatcaaaa aacgctgcca ggcgggtttt gaacggaact cgtagggttg aggcggctgc
     2941 ttcatctgct ccttgtctag cgactcatca atcatccccg agatcttaac gtagccaccc
     3001 aatggtagcc aacctattcc atactccgtt tcgccgtgct taaacctaaa aagcgagaac
     3061 catgggttaa aaaacaggta gaacttctcc acacgggttt tgaagagctt ggcaaacata
     3121 aaatgcccca tctcgtggag aatgactaaa attgaaaggc ttaaaaagaa ttgtccaact
     3181 ttaaccagta tttccattat gcaaggtttt gtgagattag ttctgccgtt ttcaagcggg
     3241 tttcaatatc tgtcatcttg taatcgtcaa gcgatggata tgcaatatag ctagattttg
     3301 acataacttc agcaattatt tcggacatct gcatgaaacc gatgctctgc tcaaggaaac
     3361 cctccacggc cacctcgtta gcagcattta acacacaagg catattacct ccacgttcca
     3421 aagcctccct ggccagcgcc aaattaggaa aacgaacagt atcgggacgc tcaaaagtca
     3481 gctgaaggtt gttgccaaag ttaatccggg ggaagtccga tttaacccga aaaggatagg
     3541 taagggcata ctgtataggg attcgcatat cgggcaaccc gagctgagct ttcaccgagc
     3601 catcctcaaa ctgaaccatg gagtggacaa ttgattgagg atgaactacc acttcaatct
     3661 ggctgggttt taggttgaag agccacctgg cctcaatcac ctcaagcccc ttattcatca
     3721 gtgaggctga atcaacagtg atcttgttgc ccatgcacca gtttggatgc tgcagcgcat
     3781 cgcttggagt aaccctccca agctgctcca gggagaaatt cctgaatggg ccacctgaag
     3841 cggtgagtat aattttctcg attgggctac gttcacccac aattgactga aaaatagccg
     3901 aatgctccga atcgactggt aaaactgatg ctttatgctc catggccaat tcagaaatca
     3961 gctcccctgc caccaccaga gtttccttat tggctagggc aattgtttta ccagctttta
     4021 tggctgaaat tgtaggttcc aaaccagcaa agcctacaag agccacaagc accatgtcaa
     4081 cttgtgccga cccaacacaa tctgcaatag agtttttccc cgcaaaaatc ttaatatccg
     4141 tcttttcgag agcgctctta acatttacgt agtacttatc attaccaatt actacggcat
     4201 taggttgata ctttagagcc tgtgaaacaa gaagtttcca gttattttga gctgtaagca
     4261 gttcaacaga aaaaagctcc ggattagatt caataacctg caacgcctgg gttccaatgg
     4321 agcctgtaga accaagaata gcaacattct tcatcggttc agctccttaa aaatggataa
     4381 actttgaagg atcgagcgtg ttaccatttt gccacagctc aaagtgtaag tgggggccgg
     4441 tggtcaactc gcccgagtta ccaattatgg caatggcatc gcctgcacct accacatcgc
     4501 cctgccgctt aagcagcttg ctgttgtgct tgtaaaccga tataaagtta ttttcatgct
     4561 gaacagcaat agtatagccg gtttcaatag tccaatcggc caaaattaca gtccccttgg
     4621 ctaccgccag aaccacctca ttaggagcag ccac
//
LOCUS       DAOPHX010000167         4554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_25586_length_4554_cov_6.104690, whole genome shotgun sequence.
ACCESSION   DAOPHX010000167 DAOPHX010000000
VERSION     DAOPHX010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25586_length_4554_cov_6.104690"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PK502_09905"
     CDS             complement(<1..1088)
                     /locus_tag="PK502_09905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HPH01173.1"
                     /translation="MDSITAVNRKALPPIFIIGALFFVFGFVTWLNGILIPYFKISCE
                     LTDFEATLVAFAFYISYTIMALPSSWILKRTGFHKGMTLGLLLMSVGTLLFVPAALSR
                     AYWMFLSGLFVMGAGLAILQTAANPYIAILGPRESAARRISIMGICNKLAGAVAPLIL
                     AYYILQDGDTFVQSLSGLDEVARMAALDSLAARVIGPYLVMTGVLVVLAIFVRYSPLP
                     EVEAEPEEVEADVAELAQKKNILQFPHLVVGAIALFFYVGVEVIAGDTIIRYGISQGI
                     AISTAKAFTSYTLFAMIVGYLLGIALMPKIISQRTALTISASLGLLFSLGVILSRGST
                     SIVLLALLGFANALIWPTIWPLAIHNLGR"
     gene            complement(1194..2624)
                     /locus_tag="PK502_09910"
     CDS             complement(1194..2624)
                     /locus_tag="PK502_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HPH01174.1"
                     /translation="MRIETDYLGPMPIPDDALYGIHSQRAKENFPDITPFHKEWYQAM
                     GLVKQACYLTYVDFAKAVKNEPELSERLIDLIPDDILHAMISAAVEIQNGNYFEHFIV
                     PAFQGGAGTSINMNVNEIIANVALLKVGRKPGDYQLIDPIEHANIYQSTNDVVPTALR
                     VAATRLLTQLEEAINGTRQVVENLEKSYRNSLRIGYTQMQAAVPSSFGQLFSTYSEAL
                     SRDWWRVSKCFERIKQVNLGGGAIGTGIGIPRFFIMEATRHLQSLTRFPFARAENLND
                     ATANLDAFVEVHATLKAHAVNLEKMLSDIRLLASDVGRNSISIPKRQVGSSIMPGKVN
                     PVITEFTVSIAHRVYSNDMLISDLAGQGVLDLNAYIPTIGHALLDSLKLLVAANHSLN
                     NFLLDGLQVDVEKTEAQLLHNPSVTTALLPIIGFNKAAILANEMRQKDIDIFEANRVC
                     QLLPDETLREAVKPENLLRLGYSLKD"
     gene            complement(2772..3854)
                     /locus_tag="PK502_09915"
     CDS             complement(2772..3854)
                     /locus_tag="PK502_09915"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HPH01175.1"
                     /translation="MKAIEFATILTILAGCTNGQKNQPRVVNSHPPIADLRLIRIEEP
                     QNGAIFPLGDNIHISIKQTGNVAPDSVVLSVNGARVERLGGLTYELSTGGLKMGTQSI
                     SLAAWKSGSRQIAGVSVKLKSNFKPEKLTYRVVRSYPHDGRAYTQGLFYHDGYLYEGT
                     GQEGESSLRKVELETGKVLRSINLGREYFGEGIALLKGMIYQLTWTSGIGFIYDFNTF
                     NNLGSFTYATQGWGLTTNGKELIMSNGSNIISFMEPSGFSEVSRIEVFDDVGPVKMLN
                     ELEYINGKIYANVYLTDRIVIINAETGAVTADVDLKGILSPSDKTGSEDVLNGIAYDF
                     QGNRLFVTGKYWPKLFEVKLVPVGKR"
     gene            complement(3952..4371)
                     /locus_tag="PK502_09920"
     CDS             complement(3952..4371)
                     /locus_tag="PK502_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01176.1"
                     /translation="MFRIAVDFDGTIVENAYPAIGKPITFAFETLRELQKRGFAIILW
                     TVRSGKLLDEAVEFCRQNGVEFYAVNRNYPEESFDGEQPRKIEADVFIDDRNLGGFPG
                     WGKAFEMLCPEELSHELEKKALSRYKKKGFLNRLLGH"
BASE COUNT         1223 a         1034 c         1069 g         1228 t
ORIGIN      
        1 cggcccaggt tgtgaatggc aagcggccaa attgttggcc aaatcagggc gttggcaaag
       61 ccaagaaggg ccagaagcac gattgatgta ctgccccttg acaaaatcac cccaaggcta
      121 aaaagcaacc ccagcgatgc tgagatggta agggctgtcc gctgggagat aatttttggc
      181 atgagcgcta tacccagcag gtagcctacg atcatggcga atagagtgta ggaggtaaat
      241 gcttttgcgg ttgatattgc aataccctgc gaaattccgt agcgaataat agtatcgccg
      301 gctattactt caacgcctac gtagaagaat agtgctatag cgccaacaac cagatgggga
      361 aactgcagga tgtttttctt ttgggccaat tcggcaacgt cggcttccac ttcttcgggt
      421 tcagcttcta cctcggggag aggggagtac cgaacgaata tggccagaac aactagcact
      481 ccggtcataa ccaggtaggg gccaataacc cttgccgcaa ggctatcaag tgctgccata
      541 cgggcaactt cgtcgagccc gctaagcgac tgcacaaatg tgtcaccatc ctgaagaatg
      601 tagtaggcca ggataagtgg ggcaactgcg ccggccagct tgttgcatat acccatgatg
      661 cttatccgtc gggctgcact ttcacgaggt cccagtatgg ctatgtaggg atttgctgca
      721 gtttgcagaa tggccaaccc ggcgcccatt acaaaaagcc cggaaaggaa catccagtaa
      781 gctctactta acgctgctgg aacaaaaagt aacgtaccta ccgacattaa cagcagccct
      841 aaggtcattc ccttatgaaa acccgttcgc ttgagaatcc atgaggaggg aagggccatt
      901 atggtgtagg atatgtagaa ggcaaaagct accagcgtag cctcaaaatc agtcagctca
      961 cacgaaattt tgaagtatgg gattagtatt ccgttaagcc atgttacaaa tccaaaaaca
     1021 aagaatagcg caccaatgat aaatatgggt ggtaaagcct ttcggttaac agctgttatt
     1081 gagtccatag aagttcggtt aggtttctca aaaatattaa atggttgaaa ttttaagcaa
     1141 tagccttcga aataatttag ggaattaatg cgattaataa ccggaataac ggcctagtct
     1201 ttaagtgagt aacccaaacg gagtaaattc tctggtttaa ccgcttccct aagagtttcg
     1261 tcgggtagaa gctggcaaac cctgttggct tcaaagatgt caatgtcctt ctgcctcatt
     1321 tcgttggcga gtattgcagc cttattgaag cctataatgg gtaagagtgc agtagttacc
     1381 gacgggttat gcagcagctg tgcctcggtt ttttcaacat caacctgtag cccatctaac
     1441 agaaagttgt ttagggagtg gtttgcggca accagcagct taagggagtc gagaagtgca
     1501 tgtcctatag tgggaatata ggcgttgagg tcaagaacac cctgacctgc aagatctgaa
     1561 atcagcatat cattcgaata aaccctatgg gcaatgctaa cggtaaactc cgtgataacc
     1621 ggattcacct taccgggcat gatggagctg ccaacctgac gcttggggat tgatatgctg
     1681 tttctgccaa catcggaggc aagtagccta atgtcggata gcattttctc gaggttaacg
     1741 gcatgggctt taagggtggc atgaacctca acaaaggcat cgaggttagc ggtggcatcg
     1801 tttaaatttt cggctcttgc aaagggaaaa cgggtgaggg attgaagatg ccgggtagct
     1861 tccatgataa aaaaccgcgg aatgcctata ccggttccaa tggctcctcc gccaaggtta
     1921 acctgcttta ttcgctcaaa acatttggac acccgccacc agtcgcgtga aagcgcttcg
     1981 ctgtatgtgc taaaaagctg gccaaatgat gaaggaactg ctgcctgcat ttgggtgtat
     2041 cctatgcgaa gcgagttgcg gtaacttttt tcaagatttt cgacaacctg acgggtgccg
     2101 ttaattgctt cctcgagctg ggtgagcagc ctggttgctg ctacccgtaa agcggttgga
     2161 actacatcgt tggttgattg gtagatgttg gcatgttcaa tgggatcgat aagctggtaa
     2221 tcaccaggct ttctaccaac ttttagaagg gcaacgttgg caataatctc gttcacattc
     2281 atgtttatgc tggtgcctgc accaccctga aatgcgggaa caatgaaatg ctcaaagtag
     2341 ttgccgtttt gtatttctac ggctgcagaa atcattgcat gtaggatatc atcggggata
     2401 aggtcaataa gcctttcgga aagttccggt tcgttcttaa cggctttggc aaaatcaaca
     2461 taggttaagt aacaggcctg cttcacaagc cccattgcct ggtaccattc cttatggaat
     2521 ggcgtaatat cgggaaaatt ctcctttgcc cgctgcgagt gtattccata tagggcatcg
     2581 tcgggaattg gcattgggcc aaggtaatct gtttctatgc gcatctacct gaatgttaga
     2641 tttttttgat ggttacatgg attctgcctg taaaaaaact tagtacgcat aaaccatctt
     2701 aaatgctgaa atcaaaaaaa ataatttaga tgaaataata gtagctaaaa gaatgtggct
     2761 taaagctgct gctagcgttt tccaaccgga acaagcttca cctcgaaaag ttttggccag
     2821 tacttacccg taacgaatag tcgatttcct tgaaaatcgt aggcgattcc atttagcacg
     2881 tcttcacttc cggttttgtc cgacgggctg agaatcccct taagatcaac gtcggctgta
     2941 actgcgcctg tttcggcgtt aataataact atcctatcgg taaggtaaac gttggcgtaa
     3001 atttttccat taatatactc cagctcgttg agcattttta caggtcctac atcgtcgaaa
     3061 acctcaattc tactcacttc cgaaaatccc gaaggttcca tgaatgagat gatgtttgag
     3121 ccgttgctca tgattagctc tttaccgttg gttgttaacc cccatccctg ggtggcatag
     3181 gtaaatgagc cgaggttatt aaacgtatta aaatcgtaaa taaaaccaat accgctagtc
     3241 catgtaagct ggtaaatcat gccctttagg agggctattc cctcaccaaa gtactccctg
     3301 ccaaggttga tgcttcttaa caccttgccg gtttccagct caaccttacg caagctcgat
     3361 tctccttcct gtcctgtacc ctcgtaaaga tacccatcgt ggtaaaatag accctgagtg
     3421 taagccctgc cgtcgtgggg gtaagagcga accaccctgt aggttagctt ctcgggttta
     3481 aagttcgact tcagcttaac ggaaacacca gctatttggc gtgagccgct cttccatgca
     3541 gcgagtgata tgctttgtgt ccccatcttt aagccaccgg ttgaaagttc ataggttaaa
     3601 cctcccaacc gttcaaccct agcgccgttg accgatagca ccaccgaatc gggggcaaca
     3661 ttgccggttt gtttgatgga gatatggata ttatcgccca ggggaaatat agcaccattc
     3721 tggggttctt caatccggat aagccggagg tctgctatgg gcgggtggct gtttactacc
     3781 cgtggctgat tcttttggcc attggtgcat ccagccaaaa tagtgagaat agtggcaaat
     3841 tcgatcgctt tcatgaactt atacatttta gctgtcggga aattatgtcg ttatgaagtg
     3901 atttgcttta ccgtatgcca aactgttagg tgttaatctg gttaacttgg gttaatgtcc
     3961 gagtaacctg tttagaaagc ctttcttttt gtatcggctt aatgcttttt tttcaagctc
     4021 atggcttaat tcttccgggc aaagcatctc aaaagcctta ccccatccag gaaatccacc
     4081 aaggtttcta tcatcaataa acacatcggc ctcaattttg cggggctgct cgccgtcaaa
     4141 cgattcttcg gggtagttac ggtttaccgc ataaaattcc acaccgtttt gcctacaaaa
     4201 ttccacggct tcatcgagga gtttacctga acgcactgtc cagagtatga tggcaaagcc
     4261 tcttttttgc aactcgcgta gagtttcaaa ggcaaaagta atgggcttac caatagctgg
     4321 gtaggcgttc tccacaattg ttccgtcaaa gtcaactgca atcctgaaca tttcaattcg
     4381 tttgacaggc caaatctacg ttaaatttag ataagaagat gccctgcact tagaaaatca
     4441 aacctgttga agcgacagct aaaggtctgc tggtcgaacc cctaaactgt gcgcttattg
     4501 taaaataatc tggctggctt actcatgcct caacctgtaa accttcctca tgca
//
LOCUS       DAOPHX010000168         4518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_25888_length_4518_cov_5.805736, whole genome shotgun sequence.
ACCESSION   DAOPHX010000168 DAOPHX010000000
VERSION     DAOPHX010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25888_length_4518_cov_5.805736"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PK502_09925"
     CDS             complement(<1..752)
                     /locus_tag="PK502_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPH01177.1"
                     /translation="MLGLSKKGNTGVSLVLSSGGARGLAHIGVIEELLTRDFCINSVV
                     GSSMGALVAGLYASGGLENFKNWAVGLSKLEMLSLVDFTLTSRGIIKGEKVFNQMQKL
                     GLIPDINIEDLPIPFAAVAVDIINGKEVVFRSGSLLKAIRASIAIPSVFTPVAHEDGL
                     LIDGGVLSPLPLQHATRSKHDLLVAVDVNSFTPYGKPIARIRRRMEQVDKPKLLKKWD
                     AFNRQHKQPMSGRAMQKGVGYLQLLTRSSLLM"
     gene            complement(821..1315)
                     /locus_tag="PK502_09930"
     CDS             complement(821..1315)
                     /locus_tag="PK502_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPH01178.1"
                     /translation="MSTKEQTLENDEKFMDEAIELAIKGVMSDEGGPFGAIVVKDGRI
                     VGKGNNHVTSTNDPTAHAEVMAIRDACRTLNTFQLNGCVLYTSCEPCPMCLGAIYWAR
                     LDKVFYSANRHIASYHGFDDSFIYNEVNLSFDKRRIPFINISPEKGLVPFREWEKKDD
                     KIKY"
     gene            1542..4397
                     /locus_tag="PK502_09935"
     CDS             1542..4397
                     /locus_tag="PK502_09935"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HPH01179.1"
                     /translation="MRFEVKFPAIHLHIILFVVFAFSLNRLQGQSALHITNYDKKEYR
                     AANQNWSVSLGGISAVFTGNNHGLLCYDGAEWLLKPVPGKSIIRAVLYDSSRVYVGLL
                     DEFGYFDIVNGKPTIYHCLSTTVSNNLKNQEYWRIAKHHGKIYFQSFGSLYVYDGREV
                     SSVPLDFPILLLIKANDELFTQRIGGGLYRITENGLKLLEGSEIFADTEIKAALTHQN
                     DILFCTNSMGIYRFNGFSFEPWHTAVDAELKTYNLNTAVETQHYYCIGTILKGVLVLD
                     HNGQLVYQLDSKHLQNSTILSLEKDKMGNIWVGKDKGISYVAFNSPVSIHIDDTDKFS
                     CYSGVLVGSYLYLGTNQGIFRYSFSSENMLTGKTLVTGSQGQVWFIKLIDGKLYCGLN
                     DGTYLLENDKLEKIGFVSGGYNLEKFSKEKDLLIQSTYNNLVTYRNNNGRWEQNKILQ
                     GFSSPSRFLQVDFQQNIWVGHSIVGVNRLQTNSNVDSIISIENIGQEHGLNFQTNRIY
                     KIENRIVIPTGSGFLRWNDLNERFEPFGELNAQLQGFEKAHAVASLPDDKYWLIKDDE
                     WGLFEIRFGKANLLYRVIPDMFNMELVEENEKIIQLNDSLQLVCLDNGFAILNILQLN
                     RLPEVNLPPNINDVRFWRNEKDSASIKPRMTRGMLISPAKFNNISITFASNEVIGTKR
                     YFQYTLEGMDGEWSTWQTSTSVSYKRLPPGDYTFKVRTLNSKGILTPAAMVKFSIQPP
                     FFLSWKAGILYICIILAISGISRNRYKKRIKQHFEQQQRQEQEKIQKEREENERVIMR
                     IQNERLRADIENKNSQLAMNTMSLIRKNALLQEFAAELENMKNELGYRIPNKYYDRIT
                     RLINQNIENEHDWETFQKLFDQAHENFFKSLKDAYPSLTTSDLRLCAYIRMNLSSKEI
                     APLLNITVRGVEERRYRLRKRLNLSTDESLNDFILKL"
BASE COUNT         1259 a         1167 c         1024 g         1068 t
ORIGIN      
        1 tgcatcagaa gggacgagcg ggtaagcagc tgcaggtagc caaccccttt ttgcatagcc
       61 ctcccggaca tgggttgctt atgctgacgg ttgaatgcgt cccacttctt taacagctta
      121 ggcttgtcaa cctgctccat tctccttctg atcctagcta taggcttgcc atacggagta
      181 aacgagttca catcaacggc aaccagcaag tcgtgcttac tccgagttgc atgctggagg
      241 ggaagaggcg aaagaacgcc tccatcaatc agcagcccat cttcatgtgc taccggagtg
      301 aaaacactag gaattgcaat tgaagcacga atagccttaa gtaaactacc gctacggaac
      361 accacctcct tgccattaat gatgtcaacc gctacggcag caaaagggat aggcaggtcc
      421 tctatgttga tgtcgggaat taaccccagc ttttgcatct ggttgaaaac cttttcaccc
      481 tttatgatac ccctggaggt tagggtgaaa tcaacaagcg agagcatctc aagtttactg
      541 agtccaacag cccagttctt aaaattctca agccctcccg aggcgtacaa accagccacc
      601 aaggcaccca ttgatgagcc aaccaccgag ttgatacaaa agtcccgggt aagtaactcc
      661 tcaataaccc caatgtgtgc taaccctctg gctccaccgc tcgagagcac cagggaaaca
      721 cctgtgttac cttttttcga cagccctaac attgcctgcc actcataatg gtgcatttat
      781 acaaccccaa cctgctgccg gaacaaaatg ggctaacggg ttagtactta atcttatcat
      841 ccttcttttc ccactccctg aagggaacca accccttttc ggggctaatg ttgataaagg
      901 gaatgcgtcg tttatcgaac gaaaggttaa cctcgttgta aatgaagcta tcgtcgaaac
      961 catggtacga ggcaatatgg cggttggccg agtagaacac cttatcgaga cgagcccagt
     1021 aaatcgctcc aagacacatc ggacaaggct cgcacgaggt gtaaagcacg cacccgttca
     1081 gctgaaaggt gttaagtgtc cggcaggcat cccttatcgc cataacctcg gcatgggcgg
     1141 taggatcgtt ggtggaggtt acatggttgt tgcctttgcc aacaatcctg ccatccttaa
     1201 ccactattgc cccaaacggc ccaccctcat cggacatcac ccccttgatt gccagctcaa
     1261 tggcttcatc cataaatttc tcgtcgttct ccagcgtttg ctccttagta ctcatctata
     1321 atcttcattt ttgttcaacc tgtaaaaata taacaaatta tccaacccca caaaggcggc
     1381 agggagtttt ggggaaaatt cagccccttt caatactgtc agcagcataa aattgaactc
     1441 tacggataag ttagccctta aaaattaccc tctgaatttt cagcgtatgc tttgctttta
     1501 ggtaataagt tctaactttg ttacgatcca atttcaaacg tatgcgcttc gaggtaaaat
     1561 ttccggcaat ccatcttcat attattcttt ttgtagtatt tgcattcagc ttaaatcggc
     1621 tacaggggca atcggcccta cacataacca actacgataa aaaggagtat agggctgcca
     1681 accaaaactg gtcggtaagc ctaggaggta tatctgccgt ttttacgggc aataaccacg
     1741 gtttactctg ttacgatggt gccgaatggc tgcttaagcc agttcccggg aaatccatta
     1801 ttcgtgccgt gctctacgat agcagcaggg tttacgtcgg cttgctcgat gaattcggct
     1861 acttcgatat tgtcaacgga aagccaacca tttaccattg cctgtcaacc acggtaagca
     1921 acaaccttaa aaatcaggag tactggcgga tagccaagca ccacgggaaa atatattttc
     1981 agagtttcgg ctcactttac gtttacgatg gacgggaggt ttcctccgtt cctcttgatt
     2041 ttccgatact tctgcttatt aaggcaaacg atgaactttt cacacagcga attggtggcg
     2101 ggttataccg cattaccgaa aatggtctta agctgttgga aggcagcgag atatttgcag
     2161 ataccgaaat taaggctgcc cttacccatc aaaacgacat cctattttgc accaactcca
     2221 tgggcattta caggttcaac gggttctcct ttgaaccctg gcacaccgcc gtggatgccg
     2281 aactcaaaac ctacaacctg aacaccgctg ttgaaaccca acattactat tgcattggca
     2341 ccattctaaa gggcgtgctg gttctggatc acaacgggca gctggtttac cagctggact
     2401 ccaagcatct gcagaacagc accattcttt cgctcgagaa ggataaaatg ggcaacatct
     2461 gggttggtaa ggataagggt ataagctatg tggctttcaa ctcacccgtg agcatccaca
     2521 ttgacgacac cgataaattc tcgtgctact ccggcgttct ggttggctca tacctctacc
     2581 tgggaaccaa tcagggaata ttccgctact ccttctccag cgaaaacatg ctcaccggca
     2641 aaacgctggt taccggttcg cagggccagg tttggtttat taagctgatt gacgggaagc
     2701 tttactgcgg gctaaacgac ggcacctacc tgcttgaaaa cgacaagctg gagaagatag
     2761 gctttgtaag cggcgggtac aacctcgaaa aattcagcaa ggaaaaggat ttgctcatcc
     2821 aaagcaccta caacaacctg gtaacctacc gaaataacaa tgggaggtgg gaacagaaca
     2881 aaatccttca gggattctca tcgcccagcc gatttcttca ggttgacttt cagcagaata
     2941 tctgggtggg gcattccatt gtgggggtaa accggctgca aaccaacagc aatgttgaca
     3001 gcatcatcag catcgagaac attggccagg agcatggcct gaattttcaa accaaccgca
     3061 tctacaaaat cgaaaataga attgtaattc caacgggttc gggatttttg aggtggaacg
     3121 acctgaatga gcggttcgag cccttcggcg agctcaacgc ccagcttcag ggtttcgaga
     3181 aggctcacgc cgttgccagc ctccccgacg acaagtactg gctgattaag gacgacgaat
     3241 gggggctttt cgaaatacgc tttggcaagg ccaacctgct ttaccgggtt attcccgaca
     3301 tgtttaacat ggagcttgtt gaggagaacg agaagatcat ccagctaaac gacagccttc
     3361 agctggtttg cctcgataat ggttttgcca tactcaacat acttcagctc aacaggctgc
     3421 ctgaggtcaa ccttcccccc aacatcaacg acgtgcgatt ctggaggaat gaaaaggaca
     3481 gcgccagcat caagcccagg atgaccaggg gaatgctgat ttctcccgca aaattcaaca
     3541 acatcagcat taccttcgct tcaaacgagg tgataggaac caaacgctac ttccagtaca
     3601 cgcttgaggg tatggatggc gaatggagca cctggcaaac atcaacctcg gtgagctaca
     3661 agcgcctgcc ccccggcgat tacaccttta aggttagaac gttgaactcc aagggaattc
     3721 tgacgccagc agccatggtg aaattctcca ttcaaccccc gtttttcctt tcgtggaaag
     3781 ccggaatact ctacatctgc atcattctgg ccatttcagg catttcacgc aaccgttaca
     3841 agaaaaggat aaagcagcat tttgagcagc agcaacgaca ggagcaggag aaaattcaaa
     3901 aggaacgcga ggaaaacgag cgggtaatca tgcgaataca gaatgagcgc ttacgggccg
     3961 acatcgaaaa caagaactcg cagctggcca tgaacaccat gtccctcatc agaaaaaatg
     4021 cccttctgca ggagtttgcc gctgagctcg aaaacatgaa gaatgagctg ggataccgca
     4081 ttcccaacaa gtactatgac cgtataacca ggctcatcaa ccagaacatc gagaatgagc
     4141 acgactggga aacctttcaa aagctgtttg accaggctca cgagaacttt ttcaaaagcc
     4201 tgaaggatgc ctacccgagc ctgaccacaa gcgatttacg gctttgcgcc tacatccgga
     4261 tgaacctctc gtcgaaggag attgcccccc tgctcaacat taccgttcgt ggtgtggagg
     4321 agcggaggta ccgccttcgg aaaaggctga acctttcaac cgacgaaagc ctgaacgatt
     4381 tcatcctcaa gctctaaaga aggttaaagg ataaaggata aaaaaattca agcaggattc
     4441 aagaactttt ccgcctcctc actgaacttg tccggtggtt ttagccccga aggggcgaaa
     4501 taattagcaa ggggcgca
//
LOCUS       DAOPHX010000169         4505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_26000_length_4505_cov_5.811910, whole genome shotgun sequence.
ACCESSION   DAOPHX010000169 DAOPHX010000000
VERSION     DAOPHX010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26000_length_4505_cov_5.811910"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1560)
                     /locus_tag="PK502_09940"
     CDS             complement(139..1560)
                     /locus_tag="PK502_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPH01180.1"
                     /translation="MEEMQQYLKHKVFSVVSRVAEVEEVRVYVIGGYVRDIFLNRTSK
                     DIDIVVVGSGIDFAQRVARLMPGARMAYFKNFGTAQLKVDDLEVEFVGARKESYRLDS
                     RKPIVEDGTLDDDLQRRDFTINTLALSLNKDTFGQLVDPFNGLKDIKNRIIRTPLDPL
                     TTFSDDPLRIMRAIRFATQLNFSIEPETLNAMLSMHERLEIVSMERKVDEVNKLMMAQ
                     RPSVGFLLMEQTGILEQLFPELAALKGVETINGISHKDNFLHTLQVLDNVAAKSDNLW
                     LRWAALLHDVAKPKTKKYIEGQGWTFYGHDYMGSRMVSGIFRRLKLPMNERMRYVEKL
                     VQMHLRPINLSQEEVTDSAVRRLLFEAGDDIDDLMTLCEADITSKNDSTVRRHLANFR
                     LVREKLKIIEEKDAIRNFQPPISGNIIMETFGIEPCHEVGVIKEAIKNAILDGKIGNN
                     FNEAYRLMLEKGMELGLKPRNER"
     gene            1779..2135
                     /gene="trxA"
                     /locus_tag="PK502_09945"
     CDS             1779..2135
                     /gene="trxA"
                     /locus_tag="PK502_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPH01181.1"
                     /translation="MIEHLTAETFKQKVFDYEKNTEWKYEGNRPAIVDFYADWCGPCK
                     MVAPILEELSKEYDGKLNIYKVDTEAEQELAGVFGIRSIPSLLFIPLNDQPQMAMGAL
                     PKDAFKKAFKDVLGVE"
     gene            2533..2874
                     /locus_tag="PK502_09950"
     CDS             2533..2874
                     /locus_tag="PK502_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01182.1"
                     /translation="MKNIIMIFILCLSYSTIAQTKQDSVFVNNQYEYLTIIIETAGSS
                     CNIFISKDSTSFDKKSFKNIEPFNLTVVTDILSNYSKEGWKVQNSNISMIGDSSPYSA
                     YIYYFLSRKKE"
     gene            2954..3757
                     /locus_tag="PK502_09955"
     CDS             2954..3757
                     /locus_tag="PK502_09955"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPH01183.1"
                     /translation="MNKYALSSLLRQVGLIKIGDKIRFYINFIKTYRLRKQFFKENRD
                     VKLPPAYYIYETFNLNYFSFYNKSIDTAKWLIGHFEKYKTLENISILDWGCGPGRVIR
                     HLPSFIDNSCKFYGTDYNQKYINWCSKNIINVSFNTNTLAPPLNYERNTFDIIYGISI
                     FTHLSKKMHFAWFNELLRVLKPGGILFLTLHGNAFKEKLTEAEKIKFEKGELIVKSNT
                     KEGHRTFCAFQPTSFFKELVGLNEILEHIPGDIKNGKPQQDVWIIKKSI"
     gene            4122..4355
                     /locus_tag="PK502_09960"
     CDS             4122..4355
                     /locus_tag="PK502_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01184.1"
                     /translation="MVEKQADTYTTLLVGTDHLGSIVGLFSTDGRLVQKLSYDACSVS
                     VGFYIKVKPATRTNPPPGGGQPPQPCQLQPALY"
BASE COUNT         1315 a          932 c          918 g         1340 t
ORIGIN      
        1 ggttaaagga taaaaaaatt caagcaggat tcaaaaactt gtccgcctac tggcggaact
       61 tgtccggctc ctgacggaac ttgtccggct cctgacggat tcacccttcg gtaggtttag
      121 agttcatgtc tcgcaatttc accgttcgtt tcgtggcttt agccccagct ccatcccctt
      181 ttccagcatc aaccggtagg cttcgttgaa gttattccct attttcccat caaggatggc
      241 atttttaatg gcctccttaa ttaccccaac ctcatggcat ggctcaatgc caaaggtttc
      301 catgatgata ttgccgctga ttggaggctg aaaattccga atggcatcct tttcctctat
      361 tattttcaac ttttcacgaa ccagtctaaa gttggccaga tggcgtcgaa cggtactgtc
      421 gtttttagag gtgatgtcgg cctcgcatag ggtcatcagg tcgtcaatgt cgtcgcctgc
      481 ctcaaatagc aaccgacgga ctgccgaatc ggttacctct tcctgtgaca ggttgatagg
      541 gcgcaggtgc atctgcacca gcttctccac atacctcatc ctctcgttca tcggtagctt
      601 cagccttctg aaaattcccg aaaccatccg gctacccatg taatcgtggc cgtaaaatgt
      661 ccaaccctgt ccttcgatgt acttcttcgt tttaggtttt gctacatcgt gcagcagcgc
      721 agcccagcgt agccaaaggt tatccgattt ggcagctaca ttgtccagca cctgcagggt
      781 atgcaggaag ttatccttat gcgatatccc gttgatggtt tcaacccctt taagggcagc
      841 cagctccggg aacagctgtt caagtattcc ggtttgctcc attagcagga atccgaccga
      901 tggccgttgg gccatcatca gcttgttcac ctcgtcaact tttcgctcca ttgagacaat
      961 ttcgaggcgt tcgtgcatgc tcagcatggc gtttagcgtt tcgggctcta tgctaaagtt
     1021 cagctgggtg gcaaatcgta tggcgcgcat aatccttagc ggatcgtccg aaaaggtggt
     1081 aagcggatca agtggtgtgc ggataattcg gttcttaata tccttaagcc cgttgaatgg
     1141 gtcaaccagc tggccaaagg tatccttgtt gaggcttaag gccaatgtgt tgatggtaaa
     1201 gtccctgcgc tgcaggtcgt cgtcgagagt gccatcctcc acaatgggct tgcgcgaatc
     1261 gagcctgtac gactccttac gggcacccac aaactccacc tccaggtcgt caacctttag
     1321 ctgggcggtg ccaaagtttt tgaagtaggc cattctggct ccgggcatca ggcgcgctac
     1381 cctttgggca aaatctatcc cgctacccac caccacaatg tcgatatcct tcgatgtgcg
     1441 gttaagaaaa atatcgcgca catatccgcc gatgacgtaa acccttacct cctcaacttc
     1501 agccacacgc gaaacaaccg aaaaaacctt atgtttaaga tactgctgca tttcctccat
     1561 gttagaaggc aaaattacta ctaattgaaa ttctgacaac ctgtttcaac ggctaaataa
     1621 tgctgaagcc ttgcatgttg gttcaaacaa ataggactag cgggttgaat gatttttttt
     1681 tggttttatg ttgaaccaac ccggcaaaat ttcgtttaat caatgtcgta tataaatatg
     1741 aaaataacta catttgtggg ttactaaatt gcataaccat gattgaacat ttaacagcgg
     1801 agacgtttaa gcagaaagtt tttgactacg agaaaaacac ggagtggaag tatgagggca
     1861 acaggccggc cattgttgat ttctatgccg actggtgtgg tccctgtaag atggttgccc
     1921 ccattctgga ggagctctcg aaggagtatg acggcaagct caacatttac aaggttgaca
     1981 cggaggccga gcaggagttg gcaggggttt tcggcatacg aagcataccc tcgctgctgt
     2041 ttatacccct caacgaccaa ccccagatgg ccatgggtgc cctaccaaag gatgccttta
     2101 aaaaggcctt taaggacgtt ttgggagtgg agtaggggag agcttcctgc agctagttct
     2161 ggcattacac aggtaaatac cccattagcg ctggtagcta taccagccct gatggggttt
     2221 cccttttgat aaatcgggaa atatttctga tagttacgaa gaactttcgc taaatgaaga
     2281 tattaatgat agtgatatca atatttatcc aaaccctact aatggagttg ttcgagttca
     2341 tcaaaattta aatattgttg aatcaattac aattaccgac cttcttggga atgtcatata
     2401 tacaaatagc aaggtttcag aaaatgattt gaatatcaat atttcaaatc atacaaaggg
     2461 attatatcta ttccatataa gaacttccaa aggaataaaa acatacaaaa ttttacttaa
     2521 ataagtgagc atatgaaaaa tataatcatg atttttatat tatgtttgag ttattcaaca
     2581 attgctcaaa caaaacaaga ttcagttttc gtaaataatc aatatgaata tttaactatt
     2641 attattgaaa cagctggttc aagttgtaat atttttattt caaaggattc tacttcattt
     2701 gataagaagt cttttaaaaa tatagaacct tttaacttaa ctgtggttac tgatatttta
     2761 tcaaattata gtaaggaagg ctggaaagtg caaaatagta atatttctat gattggtgat
     2821 agcagccctt atagtgctta tatctactat tttctttcac gaaagaaaga atagttaata
     2881 aaaagagacc cttccaaggg gtctcttttt ttaaaaaact tgtatcttta caataaattt
     2941 atattcatct tttatgaata aatacgcatt atcctctttg ctaaggcaag ttgggttaat
     3001 aaaaattggg gataaaatta ggttttacat taattttatt aaaacttatc gcttaagaaa
     3061 gcaatttttt aaagaaaata gagatgttaa actaccacca gcatattata tctatgaaac
     3121 atttaatctt aactacttta gtttttataa taaaagtatc gatacagcaa aatggctaat
     3181 tggtcatttt gaaaaatata aaactttaga aaatataagt attttagatt ggggttgcgg
     3241 tcctggaaga gttataaggc atttaccctc ttttattgat aactcttgta agttctatgg
     3301 aactgattat aatcaaaaat acattaactg gtgtagtaaa aacattatta atgtttcatt
     3361 taacacaaac acacttgctc cacctttaaa ttatgaaaga aacacttttg atattatata
     3421 tggaatctca atatttacac atctttccaa aaaaatgcat tttgcttggt ttaatgaatt
     3481 attaagggtt cttaaaccag ggggtatttt gtttttaaca ttacatggta acgcttttaa
     3541 agaaaagtta actgaagcag aaaaaattaa atttgaaaaa ggagaattaa tagtcaagtc
     3601 aaatactaag gaaggtcatc gtactttttg tgcttttcag ccaacatctt tttttaaaga
     3661 attggttggt ttaaatgaaa ttttagaaca cataccaggt gatataaaaa atggcaaacc
     3721 gcagcaggat gtatggatta ttaagaaatc aatatagttt taacttaaat ttatatacta
     3781 tggctctaaa tcgtaaatat aactttaaag acgtaaacat gcttttagaa gcaaaaacca
     3841 taacaaaatt gattattccg gacaaatgtt gccagtaatt ccgtagtaaa tgttgccacc
     3901 ttttttgcgg tgaagtttac actaatttaa aggaacattt ttagcaaaat tagcaaaaaa
     3961 attaatttgt tttttctcgt tcgtgcggaa atgttttccg tgcatctagc gctgtaatcc
     4021 gtgctggaaa taaaaaggca gttagtttat actctgggta gttttttgct tttaacccaa
     4081 aaccagctac ctttgggttg cgttccttga aacctgtggc cgtggtggag aagcaggccg
     4141 acacctacac caccctgctg gtgggcaccg accacctggg cagcattgtt gggctgttca
     4201 gcaccgatgg caggcttgtg cagaagctca gctacgatgc gtgttcggtg agtgttgggt
     4261 tttacataaa ggtaaaaccc gctacacgaa caaatccgcc acctggcgga gggcaaccgc
     4321 cgcaaccctg ccaactacag ccagcccttt actgaagtac cccgcctgcg aaggggattc
     4381 accgggcatg agcatttgga cacccttggc ctgattaaca tgaacgggag gatgtacgcc
     4441 tgtccgccca ccggcggaat ccccgtgctg ggccgtttcc tcagccccga cagctacgtg
     4501 caggc
//
LOCUS       DAOPHX010000170         4451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_26458_length_4451_cov_5.156506, whole genome shotgun sequence.
ACCESSION   DAOPHX010000170 DAOPHX010000000
VERSION     DAOPHX010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26458_length_4451_cov_5.156506"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="PK502_09965"
     CDS             complement(<1..1350)
                     /locus_tag="PK502_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01185.1"
                     /translation="MLQEITIEKTSNFWIDNGIVGLYKVLRLLPDAEDFDVRLESDKL
                     EIKALDDSFDVADFLNKAKDHVAQQYLKKTKNYGWILKNDGEFEVYRKTDFKMHLKPF
                     FTGKKPNTEGSICTPDANEKENVRRMNNDEYSQFLSFQENILSNIPEKIKLGKKGFIN
                     SPPKYEIGSNFTEEFLKKGEKLCSFSGKQFKNANTITGMNYPFLTGNSGELNFDSALK
                     VKPKISALHDFVSLFSFYNLNYLMQDDLKHYFILYDNNLKELESFYDIINANIDNLTK
                     PDFCSFETQIIGTQYESESLFNFLLSVYAQVNQRINRDKRRGILLSKSVFTLSNDGNI
                     FRDVKEYTSLNALFELLDCFENYKDDERSYREPFLNFIRYFTQRLDSGKYNTTWRNRL
                     CSDILSFRSVLKTVEWFLGEVKLKEDKGNILFLDKIIEIYNFKTQKNMKAEMVDICKA
                     "
     gene            complement(1359..2159)
                     /gene="cas6"
                     /locus_tag="PK502_09970"
     CDS             complement(1359..2159)
                     /gene="cas6"
                     /locus_tag="PK502_09970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HPH01186.1"
                     /translation="MRFSIQLTRTTGRNLLPMDYQYYISAWIYKTIKLSNDEFARFLH
                     DRGYGSDEGKLFKLFCFSRLEFGKPLLLPAEKLFQISAATLRLLISFDIPITASHFIE
                     GIFKDQELYLGDKQHGLNLRVTTVELLPEPVFLETMRYRLLTPWVVSIKEDGKPQPVY
                     LAADDERFPTMAARHLAEKHNLTHTETPAVRHEQIVVSRINGFKRSGFVISPGTPRET
                     RVVGNLFEFTLTAPITIHQMAWNAGISEKSSMGFGMIETTKENNKQEN"
     gene            complement(2223..2546)
                     /locus_tag="PK502_09975"
     CDS             complement(2223..2546)
                     /locus_tag="PK502_09975"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HPH01187.1"
                     /translation="MERRHFTNFDIAGFGYYDGPIAFSELKVGTELQLVPELNNPYDP
                     KAVIIQYKEYKLGYVPRGENDHISKLLEMGYNPFEVRIQRIDPTAKPEQQIGLVVYLR
                     KADRV"
     gene            complement(2597..3496)
                     /locus_tag="PK502_09980"
     CDS             complement(2597..3496)
                     /locus_tag="PK502_09980"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPH01188.1"
                     /translation="MDQPKLERLLHLIQLLVGNRHNTVELAKRLECSQKTIQRYIDTI
                     QNAGFVVEYHRRGVPFLSTNQGSLKEISDLVHFSPEEAYLLRIAIDSINDDTALKQNL
                     KKKLYNLYNYPWLADIIVKPEQGENVQRLIEAISDERCVILRNYRSSNSNRVDDRLVE
                     PYKFTTNYQQVWCYEHSSQTCKMFKISRIETVEILDRPWECKDKHTDARIDVFRISAE
                     SYVGTVVLRLNLRAYNLLVEEYPLSEQHITADGDNAHLFTCPVCSYEGPGRFVMGLYH
                     DVQILGDDHFISFIRQKVSALHS"
     gene            complement(3582..>4451)
                     /gene="pgeF"
                     /locus_tag="PK502_09985"
     CDS             complement(3582..>4451)
                     /gene="pgeF"
                     /locus_tag="PK502_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HPH01189.1"
                     /translation="ELVRLLADLQMPIFGMFAHGFAQRVMIYIQAQIPYYRFEQMAEH
                     SDTIVQFVSTRNGVRDNRFTIGLNGYAADSQVLANRQMLAAQHGFEPSAFVFAEQVHG
                     NVVEYISPDKRGRGAFSRSTALPHCDAMITHHPNICLVAQAADCVPILFYDPVKKAIG
                     AAHAGWRGTAAKIAAAVVGAFQERYSSDPRDILVGIGPSIGPCCYEVGDEVAQQVREA
                     FPDGWQAVLQPSPSGKQIFNLWQANRITLLAAGITPQNMEVAEICTRCNNHQFFSARA
                     GDHGRFGAFIMLR"
BASE COUNT         1113 a          987 c         1033 g         1318 t
ORIGIN      
        1 cgctttacaa atatctacca tttctgcttt catatttttt tgggttttaa aattgtatat
       61 ctctatgatt ttatccaaaa ataagatatt ccctttatct tcttttaatt taacttcacc
      121 taaaaaccat tcaaccgttt taagaacgct acgaaatgaa agtatatccg agcataagcg
      181 attacgccaa gttgtattgt actttccgct atccaatcgt tgcgtaaaat agcgaataaa
      241 attcaaaaat ggttcacggt agctgcgttc atcatccttg tagttttcaa aacaatccaa
      301 taattcgaac aatgcgttca acgaagtata ttcttttaca tcgcgaaaaa tattaccgtc
      361 attcgataag gtgaatacgc ttttgcttaa caaaattcct cttcttttat ctctgtttat
      421 gcgctgattt acctgcgcat aaacagaaag taagaaattg aataaacttt ctgattcata
      481 ttgagttcct ataatctggg tttcgaaact gcaaaaatca ggttttgtaa gattatctat
      541 gtttgcattg ataatatcat aaaaactttc aagttctttt aaattattat catacaaaat
      601 aaaatagtgt tttaaatcat cttgcattaa ataattcaga ttgtaaaagg agaagagcga
      661 aacaaaatca tgcagtgcag aaattttagg ttttactttt aatgcggaat caaaatttag
      721 ttcaccacta tttccggtca aaaacgggta attcatccct gtaattgtat ttgcattttt
      781 aaattgcttt ccgctaaatg aacatagttt ctctcctttt ttaaggaatt cttctgtgaa
      841 atttgagcct atttcatatt ttggaggtga attaataaaa cctttcttgc ccaattttat
      901 tttttcagga atgttagaca atatattttc ttgaaaagat aaaaactgag aatattcatc
      961 attattcatt cgtctgacat tctctttctc gtttgcgtct ggagtacaaa tggaaccttc
     1021 tgtatttggt tttttacctg taaaaaaggg ttttaagtgc attttaaaat cagttttacg
     1081 ataaacttca aattctccgt cattttttaa aatccaaccg taattctttg tttttttcaa
     1141 atattgctga gcgacatgat ctttcgcttt gttcaaaaaa tcagcaacat caaaagaatc
     1201 atctaaagct ttaatttcaa gtttatcact ttctaaccga acatcgaagt cttcggcatc
     1261 aggtaataag cgcagtactt tatacaagcc tacaatccca ttatctatcc aaaagttact
     1321 tgttttttct atggttattt cttgtaacat atatcctttt agttttcctg tttattattt
     1381 tcttttgttg tttcaatcat accaaacccc atgctgctct tttccgagat tccggcgttc
     1441 catgccatct ggtgtatggt tatgggcgct gttagggtaa actcaaaaag gttgcccact
     1501 accctggttt cgcgtggtgt gccggggctg attacaaatc cgcttcgctt gaagccgttg
     1561 attcggctta ccactatttg ttcatgtctt actgctggag tttcggtgtg ggttaggttg
     1621 tgcttttcgg ccaggtgtcg ggcagccatg gtgggaaatc gttcatcatc ggcagcaagg
     1681 taaacgggct gaggcttgcc atcctccttt atgctcacca cccatggtgt taacaggcga
     1741 tagcgcatgg tttcgaggaa tactggctcg ggaagaagct ccacggtggt taccctaagg
     1801 ttaaggccat gctgcttatc gcccaggtag agctcctgat ccttaaaaat cccttctatg
     1861 aaatggcttg ctgtaatggg gatgtcgaat gagattagta gcctcagggt tgctgcgctt
     1921 atttgaaaca acttctcggc gggcaggagc aagggcttac caaattcgag ccgggaaaag
     1981 caaaatagct tgaacagctt cccctcgtcg cttccataac ccctgtcgtg taggaaccgg
     2041 gcaaactcat cgttggagag cttaatagtc ttgtatatcc aggcgctaat gtagtactgg
     2101 tagtccattg gcagcaggtt gcgaccggtt gtccgtgtaa gctgtatgct gaacctcatg
     2161 ttcctatagc ctttagaaag caatagtaaa tttagataaa taacattaaa aaaaaaattt
     2221 ttttacaccc tatctgcctt cctgaggtaa accaccagcc ctatctgctg ctcgggtttg
     2281 gcagtggggt caatccgctg gatgcgaacc tcaaatgggt tgtaccccat ctcgagcagc
     2341 ttgctgatgt ggtcgttctc accccggggc acgtagccca gcttgtactc cttgtactgg
     2401 ataataaccg ctttggggtc gtaggggttg ttgagctcgg gaaccagctg cagctcggtg
     2461 ccaaccttaa gctcgctgaa tgcaatgggg ccgtcgtagt agccaaagcc cgcaatgtcg
     2521 aaattggtga agtgtctccg ttccataaca ttaattttta tgccaagcta cacctgcaat
     2581 tcgacacctt ttgtcgctag gagtgaaggg ctgaaacttt ttgacgaatg aacgaaatga
     2641 agtggtcatc gcctaaaatc tgcacatcgt ggtacagccc catcacaaac cgcccgggac
     2701 cctcgtagct gcatacaggg caggtgaaca ggtgagcgtt gtcgccgtcg gcagtgatgt
     2761 gctgctccga aagggggtac tcctcaacca acaggttgta ggcccgtagg ttgagtcgta
     2821 ggactaccgt gccaacgtag ctttcggccg agattcggaa cacgtcaatt ctggcatccg
     2881 tgtgcttatc cttgcactcc catggcctat cgagaatttc aacggtttcg attctggaaa
     2941 tcttaaacat tttgcaggtt tggctggagt gctcgtagca ccacacctgc tggtagttgg
     3001 tggtaaactt gtagggttcc accagcctgt cgtcaaccct gttgctgttc gaggagcggt
     3061 aattcctgag aatgacgcat ctctcgtcgc tgatggcctc aatcaggcgc tgcacgtttt
     3121 cgccttgttc gggcttaaca atgatgtcgg ccagccaggg gtaattgtag aggttgtaga
     3181 gcttcttttt caggttctgt ttcagggcgg tgtcgtcgtt aatgctgtcg atggcgatgc
     3241 ggagcaggta ggcctcctcg ggagagaagt gcacaaggtc gctgatctcc ttaaggctgc
     3301 cctggttggt tgaaagaaat gggacaccgc gccggtggta ctccaccaca aaaccggcgt
     3361 tctggatggt atcaatgtac cgctgaatgg ttttctggga gcattcgagc cgtttggcca
     3421 gctcaactgt gttgtggcgg ttgcccacca gcagctgtat caggtgtagc aacctttcca
     3481 gcttgggttg gtccatggtg tagggggtgt tggtttgcca ttaaaggtag cagctggttt
     3541 tgtgaaatcc aaacctttac cacaggtggt tgtttcggca gctacctgag catgatgaag
     3601 gcgccaaacc gtccgtggtc accggcgcga gccgagaaaa actggtggtt gttgcacctg
     3661 gtgcaaattt cggcgacttc catgttttgt ggtgtgattc cggctgccaa aagggtgatg
     3721 cggtttgcct gccagaggtt aaaaatttgt tttcccgagg gggaaggttg aaggactgct
     3781 tgccacccat cggggaatgc ctccctaacc tgttgggcca cctcgtcgcc aacttcgtag
     3841 cagcatgggc caatggaggg gccaattcct accagaatgt cccggggatc ggagctgtaa
     3901 cgctcctgaa aggcaccaac aaccgctgct gcaattttag cagctgttcc cctccaaccg
     3961 gcatgggcag caccgatagc cttcttgacc ggatcgtaga agagaattgg aacacagtcg
     4021 gcagcctggg ctaccaggca gatgttggga tggtgggtta tcatggcgtc gcagtggggt
     4081 agggcagttg aacggctaaa tgcacctctg ccccttttgt cgggtgagat gtactccacc
     4141 acgtttccat gtacctgctc ggcaaatacg aaagccgatg gctcaaaccc atgctgggca
     4201 gccagcatct gcctgttagc cagaacctgg ctatcggcgg cgtaaccgtt aagcccaatg
     4261 gtaaagcggt tgtcccttac accattccgg gtagagacaa attgcacgat ggtgtcggaa
     4321 tgctccgcca tttgttcgaa acggtagtag ggtatttgag cctgaatgta gatcatgaca
     4381 cgttgggcaa aaccatgtgc aaacatacca aaaattggca tttgcaaatc cgccagtagg
     4441 cggacaagtt c
//
LOCUS       DAOPHX010000171         4448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_26476_length_4448_cov_5.758024, whole genome shotgun sequence.
ACCESSION   DAOPHX010000171 DAOPHX010000000
VERSION     DAOPHX010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26476_length_4448_cov_5.758024"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="PK502_09990"
     CDS             <1..1260
                     /locus_tag="PK502_09990"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HPH01190.1"
                     /translation="IHESIVKDVVFRGTNLWILMFAIIIASVGLNMNSTAVIIGAMLI
                     SPLMGPINGMGYSIATYNMKLFKISLKNYGFAVLAGLFASTLYFSISPISTAHSELLS
                     RTTPTIYDVIIALFGGLAGIVAISSKQKGNVIPGVAIATALMPPLCTAGYGLATGQFN
                     FFFGAFYLFTINTVFIGISSVAISQILKFPIIADMDVRQKKSINRWISTVIIVVLLPS
                     IYFGYRLVQNEKFNLNASRYISNVSSFEGNYLLRNNIDVRKKIITLTYGGVSLTEKQK
                     EAITQKASNFDLDDAKILIEQGLALNSSNETDSEVIKLRSELTRLNMIIRDKDAQIDS
                     ISRKRQLGKTLLLEIQAFFPKISSCSYAETFQYKTDSSQPDKTTVVFFGITSGITPAE
                     KKQISNWLNARLNQDDIKVFWEYVPKN"
     gene            complement(1403..1861)
                     /gene="pyrI"
                     /locus_tag="PK502_09995"
     CDS             complement(1403..1861)
                     /gene="pyrI"
                     /locus_tag="PK502_09995"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HPH01191.1"
                     /translation="MKDKKHLSVSAIENGTVIDHIPAQNLFKVISILGLDRIPNQITF
                     GTNLESQRLGRKAIVKIADKYFEREEINRIAIVAPHAKLNIIRDYEVVEKMLVEVPDH
                     ITGIVRCVNPKCITNFENVAPKFEVVDKKNVSLKCHYCEKITDQEHMEYR"
     gene            complement(1947..2855)
                     /gene="pyrB"
                     /locus_tag="PK502_10000"
     CDS             complement(1947..2855)
                     /gene="pyrB"
                     /locus_tag="PK502_10000"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HPH01192.1"
                     /translation="MKSKSLVSINDLSRDEMLRVLDLAEEFERNPSQPILQGKVVATL
                     FFEPSTRTRLSFESAVNRLSGRVIGFSEASSSSVSKGESLKDTILTVANYSDLIVMRH
                     PVEGSARYASEVSPVPIINAGDGANQHPTQTLLDLYSIRKTQGTLNDLKIVMVGDLKY
                     GRTVHSLLMAISQFNASFVFIAPPELQMPQEYKLFLKESGLKFEEHTDLADNIVDADI
                     VYMTRVQRERFSDPMEYEKVKNVYVLRNHMLANTKPNLKVMHPLPRVNEISIDVDDSS
                     KAYYFTQALNGVYTRMAIMSLILGLK"
     gene            complement(2919..3530)
                     /locus_tag="PK502_10005"
     CDS             complement(2919..3530)
                     /locus_tag="PK502_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPH01193.1"
                     /translation="MQADDTYQTIELKAEGLYKEKGSRFIALAFPVSSEVQVKAILDD
                     LRQEYHDARHHSFAYIVGYKGERWRTNDDGEPSGTAGKPIYGQLLSFNLTNVLVVVVR
                     YFGGTKLGVSGLINAYKTAACNALNNARIVTKTIDQTFSVFVDYPLVGEVMRVIKDLS
                     IQVIEQQFDTRCMVRVSVRQSLCGEFTSRISKVESAWIDSVDQ"
     gene            complement(3530..>4448)
                     /locus_tag="PK502_10010"
     CDS             complement(3530..>4448)
                     /locus_tag="PK502_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01194.1"
                     /translation="KILVILPKPDAMLYLPLCILSSAVILVVFKIAERMKINTLSIIL
                     INYIIACLAGFFLFRGEGAFSGQFSPGSIAFMVCLGVLLVVVFRLVGLSTQRAGIAAT
                     SVASKMSVVLPILFSIWIDPHDKLTSTKLAGIVVALVAVILSVYRNDNPRDSLRKIYL
                     PVLIFFGIGTIDSSVKYAQATFVTGELNPLFNFVIFGVAFIAGFAMVAFSKPALKDLQ
                     HLKTWLVGVVLGLANFGSMYFMIAALNHINPATGKPAQGSVVFGINNIGVVVLGVLLG
                     YTFFKERPTRLNWSGIALSVVAIFILMNS"
BASE COUNT         1222 a         1053 c          984 g         1189 t
ORIGIN      
        1 attcatgaaa gcatcgtgaa ggacgtggtg ttcaggggaa ccaacctgtg gattttaatg
       61 tttgctatca ttatagcatc ggttggcctg aatatgaact ctacagcggt aattataggc
      121 gctatgttga tatcgccact aatggggcct ataaatggta tgggctacag catagcaacc
      181 tacaacatga agctctttaa aatttcttta aaaaattatg gttttgccgt gcttgccggg
      241 ctttttgcct caaccctcta tttttcaata agtccaatat caactgcaca ttcagagctg
      301 ctatcccgaa caactcccac aatctatgac gtaataattg ctctttttgg cgggctggcc
      361 ggaatcgttg ccattagcag taagcaaaag ggaaatgtta tccccggtgt tgctattgct
      421 acagcgctta tgcctcctct ttgcacggca ggctacggat tggccacagg gcagttcaac
      481 tttttctttg gggcgttcta cctcttcacc attaatacgg tttttatagg tatttcatct
      541 gtagccattt ctcaaattct taaatttccg ataattgctg acatggatgt caggcaaaag
      601 aaaagcataa acagatggat ttctacggta ataatcgtgg tgctgcttcc gagcatttat
      661 tttggctaca ggctggtgca aaatgagaaa tttaacctga atgctagccg gtacatttcg
      721 aatgtaagtt cctttgaggg caactaccta cttagaaata acattgatgt acgtaaaaaa
      781 atcatcacgc taacctatgg aggtgtaagc ttaactgaaa agcaaaaaga agcgattacc
      841 caaaaggcga gcaattttga tctcgatgat gccaaaatac ttattgagca aggtttagcc
      901 ttgaatagct caaatgaaac cgacagtgag gttattaagc ttaggtctga gctaacccgc
      961 ttaaatatga ttatcaggga taaagatgcc caaattgata gtatctccag gaaaaggcag
     1021 ctaggcaaaa ccctcctgct ggagattcaa gcttttttcc ctaagatttc cagctgctcc
     1081 tatgccgaaa ccttccagta taaaaccgac agttctcaac ccgataaaac cacggtagta
     1141 tttttcggta taacctccgg gataacccct gccgaaaaaa agcagatttc caactggctc
     1201 aatgccaggt tgaatcagga cgacatcaag gttttctggg agtatgtgcc taaaaattaa
     1261 tttttaattc attggcatat tgtttcgcac tacatgatgc tttctaccac tactgctcgg
     1321 gccagaagtt cagcataatt ttctacagcc ttcgattagc ggcgaaacct ccttccaaag
     1381 tattccatct ataattcccg ccctagcggt attccatatg ttcctgatcg gtgatttttt
     1441 cgcagtaatg gcatttcagg ctaacatttt tcttgtcaac cacctcaaat tttggggcaa
     1501 cattctcaaa gttggtaatg catttagggt tcacacacct aactatgccc gtaatatggt
     1561 cgggaacctc aaccagcatt ttctcaacca cctcataatc cctgatgatg ttgagcttgg
     1621 catggggcgc cacaatggct attcggttaa tctcctcgcg ttcaaagtac ttgtcggcaa
     1681 ttttcactat ggcctttcta cccaaccgct ggctttccag attggttcca aaggtaatct
     1741 ggttggggat tctgtcaagt ccaagaattg agataacctt aaataggttc tgagcgggaa
     1801 tgtggtcaat aaccgttccg ttttcaattg cacttacact taaatgtttt ttgtctttca
     1861 tcgaattaat ttattagctg gttaaattgc aacgaattat aaaatataca ctaccacgcc
     1921 atgcattccg ccatggcacc tcgtagctac ttcagtccaa gaattagcga catgatggcc
     1981 attcgggtgt acaccccgtt tagggcctgg gtaaagtagt aggctttgga gctgtcgtcc
     2041 acatcaatgc taatttcgtt tacccgcggc agcggatgca tcaccttcag gttgggcttg
     2101 gtgttggcta gcatgtggtt gcgaagcacg taaacgtttt ttaccttttc gtactccatg
     2161 gggtccgaaa aacgctcgcg ctgcacacgg gtcatataca caatgtcggc atcaacaata
     2221 ttatcggcca ggtcggtgtg ctcctcaaac ttcaggcccg attccttaag aaacagcttg
     2281 tactcctgag gcatttgcag ctcgggaggg gcaatgaaaa caaaggaggc gttaaactgc
     2341 gaaatggcca tcagtagtga gtgaacagtt cggccatact taaggtcacc caccatcaca
     2401 atctttaggt cgttgagggt tccctgtgtt ttgcggatgg agtacaggtc aagcagcgtt
     2461 tgggtggggt gctggttggc gccatcgcct gcattaataa tcggcaccgg cgatacctcg
     2521 ctggcataac gggcgctacc ctccaccgga tgccgcatta caatcaggtc ggagtagttg
     2581 gctactgtta gaatggtatc cttgaggctt tcaccctttg aaacgctact cgacgaggcc
     2641 tcggaaaacc caataaccct gccgctcaac ctgttaactg cgctctcgaa gctcagacgg
     2701 gtacgggtag atggctcaaa gaaaagtgta gcaaccactt tgccctgtag aattggttgg
     2761 ctagggtttc tctcgaattc ctccgccagg tcaagtaccc tgagcatttc atccctactc
     2821 aggtcgttaa ttgaaactaa acttttactt ttcatgctca tgttgttttt aggttatatg
     2881 tagaaaaaaa gccccggtta acagggcttt acaaacaatt actggtcaac gctgtctatc
     2941 catgccgatt ccaccttgct gattctggag gtaaactccc cgcaaaggct ttggcgcacc
     3001 gatacgcgca ccatgcaccg ggtatcgaac tgctgctcaa tcacctgaat acttaaatcc
     3061 ttaataaccc tcatcacctc acccaccaag gggtagtcaa caaaaactga aaatgtttgg
     3121 tctattgttt tggttacaat ccgggcattg ttgagggcgt tgcaggcggc agttttatat
     3181 gcgttaatca gacccgaaac gccaagcttt gtcccgccaa agtatcgtac caccaccacc
     3241 agtacgttgg tcaggttaaa actgaggagc tgtccgtata ttggcttgcc agcagtcccg
     3301 gagggttcac catcgtcgtt agttctccac ctctcacctt tatagccaac tatgtatgca
     3361 aagctgtgat ggcgggcatc atggtactcc tgcctaaggt catcgagaat agcctttacc
     3421 tgcacctcgg aagatacggg aaaagccagc gcaataaacc ggctaccctt ttccttgtaa
     3481 aggccttcgg ctttcagctc aatagtttgg taagtatcgt cagcctgcat cagctattca
     3541 ttagaataaa aattgcaaca accgacaggg cgattcccga ccagttgaga cgggttgggc
     3601 gctccttaaa aaatgtatat cccagaagca ctccgagaac caccacgcct atattgttaa
     3661 ttccaaacac aaccgaaccc tgggcaggct tgcctgtagc cgggttgatg tggttgagag
     3721 cagcaatcat gaagtacatc gagccaaagt tggctagtcc cagtactact ccgactagcc
     3781 acgttttaag gtgctgcaaa tctttcagcg ctggcttcga aaaagccacc attgcgaaac
     3841 cggcaataaa cgcaactcca aaaattacaa agttgaaaag cgggttcagc tcgccggtaa
     3901 caaaggtggc ctgggcatac ttcaccgacg agtcaatagt tcctatccca aaaaaaatga
     3961 ggacaggaag gtatattttc cgaagtgaat ccctggggtt atcgtttcgg taaacagaaa
     4021 ggataactgc taccaacgca accactatgc cggcaagctt tgtggaggta agcttatcgt
     4081 gtggatcaat ccagatagag aacaggatgg ggagaacgac cgacatcttg gaggcaaccg
     4141 aggtagctgc aattcccgca cgctgggtgc ttaagcccac caatctaaaa accaccacga
     4201 gaagtactcc cagacaaacc atgaaggcaa tactgccagg gctaaattgc ccagaaaagg
     4261 caccctcacc cctgaacaaa aaaaaacctg ctaggcaggc aatgatgtag ttgatgagaa
     4321 tgatgctgag ggtgttgatt ttcatccttt cagcaatctt gaaaacaacc agtattacag
     4381 ccgaggaaag gatgcagaga ggtaggtata acatcgcatc aggttttggc aaaattacaa
     4441 gaattttg
//
LOCUS       DAOPHX010000172         4420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_26727_length_4420_cov_6.081787, whole genome shotgun sequence.
ACCESSION   DAOPHX010000172 DAOPHX010000000
VERSION     DAOPHX010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26727_length_4420_cov_6.081787"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(317..1711)
                     /locus_tag="PK502_10015"
     CDS             complement(317..1711)
                     /locus_tag="PK502_10015"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPH01195.1"
                     /translation="MRAELLKKRALTRMVRLASNLSDEKLIRMVELSQRHLMRGVTDE
                     RVRFTAEKIKKLFEEKHPATELAKSTFRRVSKESRKRIVENFFINAGVTGRQRQEEMK
                     RELGFGMPWFFVISPTAKCNLKCKGCYAGEYTKSEDLSFELVDRIFTEAKELGMYFIT
                     ISGGEPYMWPPLLDIFRKHSDMFFQTYTNGTLLTKDMAQKLAKLGNVAPAISVEGFEK
                     ETDARRGNGVFQRVMDAMDNLREAGVVFGYSVTPTRLNSELLVSDEFVDLMIQKGCVF
                     GWYFQYVPIGRKPDVSLMATPEQRNYIRQRTKEIRNTKPIFIGDFWNDGPIVGGCLAG
                     ARHRGYFHINCKGDVEPCVFLQFSVDNIKDKKLLDVIQSPFFRAIQDAQPYCENKNLL
                     TPCALIDNPQVLRDVVKQHNARPSYNSSYEVISNPDVCNFLDAYSKDYKKITDPIWEN
                     EVSLKYQSWKERFS"
     gene            2139..4058
                     /gene="dxs"
                     /locus_tag="PK502_10020"
     CDS             2139..4058
                     /gene="dxs"
                     /locus_tag="PK502_10020"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPH01196.1"
                     /translation="MGSDYRILPHVNSPNDLKGMKPEQLVELSEELRRFIVEVVSTNP
                     GHLGASLGVVELTVALHHVFNTPDDKLIWDVGHQAYAHKILTGRRDVFNTNRRYKGIS
                     GFPKRSESPYDAFGVGHASTSISAALGMAVASELKHESHQVVAIIGDGALTGGLAFEG
                     LNNGGSLKSNLLVILNDNHISIDPGVGALNEYLLDITTSKTYNRIKKDVWDLLGRFDS
                     LGPKARSAIQKLDNAIKTALLRQSNLFESLGFRYFGPVDGHDVAYLIRILNDLKDIPG
                     PKLLHCITVKGKGYTPAEQNQTIFHAPGKFNKDTGERISLLTSKPAPPRYQDVFGETL
                     VELAQSNDRIVGITPAMATGCSMNIMMEKMPERTFDVGIAEQHAVTFAAGMAADGLIP
                     FCNIYSSFMQRAFDQVIHDVALQNLHVVFCLDRAGLVGDDGPTHHGAFDLAYLRLIPN
                     ITIAAPMNEEELRNMMFTAQLDGQGTFSIRYPRGNGVMVDWRKSFTEIPIGKARILRE
                     GSDIAILTIGHVGNLAATAAGKLEAEGISVFHADMRFVKPLDADLLRSVAKRFTTVVT
                     VEDGCLPGGFGSGVTEWLNDNGYNVRVVRLGIPDEFIEQGPPQQLYAECGFDTENIYQ
                     TAKKYVQKKVKMRVG"
BASE COUNT         1194 a         1082 c          992 g         1152 t
ORIGIN      
        1 ttgattaagg tgggttctaa aaattaactt cgttgagctc gcaagctcgg ttcatctttt
       61 tgcaaaaatt tttctatcta tctactactt aacattgcaa aatatttaac cgaacaactt
      121 gttcgtatcg gaataaatta tacatccctt tgctgccgca cggttgcctt tgccgagctc
      181 tcccacggcg ggcgggtagg ttcgtggagt agcggggttt tatgccccaa agcatttcgg
      241 ggcaaatcat acccctcaac cggagttggc cgaagcatgc cggcactgaa catcctacga
      301 atggacaaaa tttctgctag ctgaagcgtt ccttccagga ttggtacttc aagctaacct
      361 cgttttccca aattggatcg gtaattttct tgtaatcctt tgaataggca tctaaaaaat
      421 tacaaacatc ggggttagaa atcacctcgt agctgctgtt gtaggagggc ctggcgttgt
      481 gctgcttaac aacatccctt aaaacctggg gattatcaat tagagcgcag ggggtgagaa
      541 ggttcttatt ctcacaatag ggttgggcat cctgaattgc cctgaagaat ggagattgta
      601 taacgtcaag cagcttttta tccttaatgt tgtccaccga aaactgcagg aaaacgcagg
      661 gctccacgtc gcccttgcag ttgatgtgga agtatcccct gtgcctggca ccggcaaggc
      721 atccgcccac aattggacca tcgttccaga agtcgccgat aaaaattggc tttgtattcc
      781 ttatctcctt ggtgcgctgc ctaatatagt ttctttgctc gggcgttgcc atgagcgaaa
      841 catccggttt acggccaatt ggcacgtact ggaagtacca gccaaacaca cacccctttt
      901 gaatcatcaa gtcaacaaac tcgtcggaaa ctagcaattc ggagttgagc cgtgttggtg
      961 taacagagta tccgaatacc acaccggcct cacgcaggtt gtccatggca tccatcaccc
     1021 tttgaaaaac accgttgccc ctacgggcat ccgtctcctt ctcaaacccc tccaccgaaa
     1081 ttgccggtgc cacgttgccc agcttcgcca acttttgggc catatcctta gttaacagcg
     1141 tgccgttggt gtaggtttgg aaaaacatat cgctatgctt cctgaaaata tcgagcagag
     1201 gaggccacat gtaaggttcc ccacctgata tggtgataaa gtacatcccc agctctttgg
     1261 cctcggtaaa aatgcggtca accagctcga atgaaaggtc ctcggactta gtgtactccc
     1321 cggcatagca ccccttgcac ttgaggttgc actttgccgt tgggctaata acaaaaaacc
     1381 acggcatgcc aaatcccagc tcacgcttca tctcctcctg cctttgcctt ccggtaaccc
     1441 ccgcattgat aaagaaattt tcaactattc gctttcggct ctccttcgat actcttctga
     1501 atgtgctttt ggccagctcg gttgcgggat gtttctcctc aaagagcttc ttaatctttt
     1561 ctgccgtaaa cctcaccctt tcgtcggtaa cccctctcat gaggtgccgc tgcgaaagct
     1621 ccaccatcct aatcagcttt tcgtccgata gatttgaagc cagcctaacc atccgggtta
     1681 atgctctctt ctttaaaagt tctgctctca tggcttaaaa aattaatgaa caaattcgta
     1741 taagaaaaac ttattttaaa aacctgaata ggttattgaa aattcaagcc atccctttat
     1801 tctatgagcc ttgaacgtgt tcaaaaggta gtgaataaaa ccataggcat ccagcctttt
     1861 aacataattt tagatttttt aatactgatg agagggcaac cccccatgta acatattctg
     1921 tctgtaatta aagtgttata ggacaaccca ttagtataaa tcaaacataa aaagcagaag
     1981 cttacataag cctaaatgat gaacgcatca aaattatgat ggtacttaag ctaacccttt
     2041 cactcgtcag gtagcaactc cgtgtgccct ataaatattt tcagaaaaga gcaacatttt
     2101 cttaactttg cggttaaagg aatataagtg gagcagcaat gggcagcgac tatagaatat
     2161 taccccatgt taactcgccc aacgatctga aggggatgaa accggagcag ctcgttgagc
     2221 ttagcgagga gttgcgcaga tttatcgttg aggtggtttc aactaatccc ggccacctag
     2281 gagccagcct gggggtagtt gagctgacag ttgcccttca tcatgtattc aatacacccg
     2341 acgacaagct aatttgggat gttggccacc aggcctacgc acataaaatt cttacgggca
     2401 gaagggatgt tttcaacacc aatcgcaggt ataaaggaat aagcggtttc ccaaaacgga
     2461 gcgagagccc ctacgatgca tttggcgttg ggcacgcctc aacctccatt tctgctgcac
     2521 tgggaatggc ggttgcctca gagttaaaac acgaatcaca ccaggttgtt gccattattg
     2581 gtgatggagc gctaaccggc ggattggcct ttgaagggct taacaacggc ggtagcttga
     2641 agtcgaacct gctggttatt cttaacgata accatatatc tattgacccc ggggttgggg
     2701 cgctaaacga gtacctcctc gacataacca cctccaaaac ctacaacagg ataaagaagg
     2761 atgtttggga tttactggga aggtttgaca gcttaggccc caaggcacgt tcggctatcc
     2821 agaaactcga caatgctatc aaaacagccc tgctcaggca aagtaacctg tttgaatctt
     2881 tgggatttcg ctactttggc cctgttgacg ggcacgatgt ggcctacctg atacgaattt
     2941 taaacgattt gaaggatatt ccgggtccta aactcctgca ctgcataacc gttaaaggaa
     3001 aaggctatac tcctgccgag caaaaccaaa caatcttcca cgctcccgga aaattcaaca
     3061 aggacaccgg tgagcgaatc agcctcctta ccagcaagcc agccccacca aggtatcagg
     3121 atgtttttgg tgaaaccctg gttgaactcg cccaaagtaa cgaccgaata gttggaatta
     3181 ctcctgctat ggccaccgga tgctccatga acattatgat ggagaaaatg ccagaaagaa
     3241 catttgatgt gggcatagcc gagcaacacg ctgttacatt tgctgccggt atggctgccg
     3301 acggccttat accgttctgc aacatttact ccagcttcat gcagagggca ttcgatcagg
     3361 taatacatga tgtagccctt caaaatctcc acgtggtgtt ttgccttgac cgtgccggac
     3421 tcgttggtga tgatgggcca acccaccacg gagctttcga cttagcctac ttaaggttaa
     3481 tccccaatat cactattgcg gctcccatga acgaggagga gttgcgaaac atgatgttta
     3541 ctgcccaact tgatgggcag ggtacattct ccatacgcta ccccagggga aatggggtaa
     3601 tggttgactg gcgcaaatca ttcaccgaaa tccctattgg caaggcccgt attcttcggg
     3661 agggttccga catcgccatc ctgactattg gccatgtggg taatcttgct gctactgcag
     3721 ccgggaagct tgaagccgaa ggcataagcg tttttcatgc cgacatgcgt tttgttaaac
     3781 ccctggatgc cgacctgcta cgctccgttg ccaagcggtt tacgactgtc gtaaccgttg
     3841 aggatggttg tttacctggt ggttttggaa gcggtgtaac ggaatggctt aacgataacg
     3901 gctacaacgt tagggtggtt aggcttggaa tccccgacga gttcattgag cagggtcctc
     3961 cccagcagct atacgccgaa tgcggctttg atactgagaa tatctaccaa actgccaaga
     4021 aatacgttca gaaaaaggtt aaaatgcggg tagggtaaaa ttttacatcg agctggcact
     4081 cgcaatgaat ttctctgaaa gcctttgtga cgcttaaaat ccatactaca caattaagac
     4141 taactacacg ctgtaattat agcatggcgt tgaggcagta agcctattaa atggccttta
     4201 attcaaacta acgttgaaaa aggcattaaa aacacaactt tggtgggtat gagttgccct
     4261 taaatgttga agtattcaac ctttcaaata caagtttgcg cccggcagaa aatattgcaa
     4321 acacaggcgc aaacaaatac aacaacggga acccatcagg gttaatggca aaccgaaagc
     4381 atgcgggcct cattcatcag cttgcgggtt agctcccgta
//
LOCUS       DAOPHX010000173         4400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_26886_length_4400_cov_5.305869, whole genome shotgun sequence.
ACCESSION   DAOPHX010000173 DAOPHX010000000
VERSION     DAOPHX010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26886_length_4400_cov_5.305869"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..2395
                     /locus_tag="PK502_10025"
     CDS             155..2395
                     /locus_tag="PK502_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPH01197.1"
                     /translation="MAVSNENLNGQKLNLEILIAMKFKPKSLFVVAVALVAISSTAIA
                     QKQAITLDDLFKKGTFRARGVYGLTSMNDGKSYSAYNSKGQVAKFEYSTGKEVEVIFD
                     PATVNVEDMNSFSGYQLSPDETIMLVETNVESIYRHSYTAVNYVYNLKSREIKPLSVN
                     GPQMNAAFSPDGRMVAFVRNNNIYMVNLTTAEEFQVTTDGLYNSIINGAADWVYEEEF
                     SLTTGLQWSPDSRKLAFYRFDESRVKMFNMTKYEGNLYPSNYTFKYPKAGEENSVVSI
                     HVFNLDSKSTIKMDVGPETDQYIARIKWSADPEKLCMVRLNRLQNKVDILLADASTGS
                     SSVLYTETNKYYIEEVTDAYPTFTPDGKYFIITSEKDGYRHIYLYGMNGSLVNQLTAG
                     INEVTEMYGYSPLSKMVYYQAYDDSPLCKAVYAKKIDGKQPVKLSTNIGNNYAQFSSN
                     FEYCMIFHSNIQEPLTVSLINAKGKEVRVVEDNQKLRDRLKEFAIPSKEFYTFTTSEG
                     VELNGYMIKPVDFDSTRKYPVMMYQYSGPGSQTVTNSFRVGWDEYLATLGYLVVCVDG
                     RGTGGRGEAFRKMTYGQMGYYESIDQIEAAKYLQTLSWVDAGRIGIWGWSYGGYMSSL
                     CLFKGADIFKMAIAVAPVTNWRYYDSIYTERFMGLPQDNPLGYDSNSPINHADKLRGK
                     LLLVHGTADDNVHFQNSMELVERLVQSGKQFDMMIYPDKNHGIYGGNTTMHLYTLLSN
                     YVQENL"
     gene            2467..2550
                     /locus_tag="PK502_10030"
     tRNA            2467..2550
                     /locus_tag="PK502_10030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2501..2503,aa:Leu,seq:cag)
     gene            2666..2752
                     /locus_tag="PK502_10035"
     tRNA            2666..2752
                     /locus_tag="PK502_10035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2700..2702,aa:Leu,seq:gag)
     gene            2809..3543
                     /locus_tag="PK502_10040"
     CDS             2809..3543
                     /locus_tag="PK502_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPH01198.1"
                     /translation="MRKVKLTLLLVSAFTGSFAQSADSFTTPNGKSLRITFIGHGTLM
                     MEYENLVIHVDPWSKLASYDKLPKADIVLVTHHHADHLDSAALVKVCKVGTEIYWTQT
                     CSGSSSLKPKSTIMHNGDRLTTHGVEISAVPAYNLIHMTPGGTPFHVKGEGNGYILNF
                     DGFVVYVAGDTEDIPEMKGFGRVDIAFLPMNLPYTMTPEMVHNAATMLHPKILYPYHY
                     GDTDTNALLNIMKTMPEVDVRIRNLK"
     gene            complement(3887..>4400)
                     /locus_tag="PK502_10045"
     CDS             complement(3887..>4400)
                     /locus_tag="PK502_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPH01199.1"
                     /translation="TRSEQSEMVDIPIVVDGRNFVVKLGGRIDRVEQANGLTRIIDFK
                     TGKFEPNKVTFSEIDQLFENKSNTDGILQLFAYCMIYSMKYGIDAGSIQPNLWYVRKN
                     TLPLISKSLKKGSSAKTEVTTFESLQAPFRQMLVALLEEIFNPSVDFTQTDNTDNCKI
                     CPYNAICNRV"
BASE COUNT         1222 a          965 c          977 g         1236 t
ORIGIN      
        1 cgaactactc actgtaacac ctgtcgaagg gtgaatccgc cagtaggcgg acaagttctt
       61 gaatcttgaa tcttaaattt tgaatcttga atttttttat cctttaccct ttatctttat
      121 actccgttat gtttatttaa actatttggc tacattggca gtcagtaatg aaaaccttaa
      181 cgggcaaaaa ctaaacctag aaatactgat tgccatgaag tttaaaccaa aatcactatt
      241 tgtggttgct gttgcacttg ttgccatcag cagcacggct attgcacaaa aacaggcaat
      301 tacgcttgat gatttgttta aaaaaggcac ctttcgtgct cggggcgttt acgggttaac
      361 ctccatgaac gatgggaaga gctacagcgc ctataattcc aaagggcagg ttgccaaatt
      421 tgagtattct accggtaaag aggttgaagt gatcttcgac cctgctactg ttaatgttga
      481 agacatgaac tcattttcgg gttaccagct tagtcccgat gaaaccataa tgcttgtcga
      541 aaccaacgtt gagagtattt ataggcattc atacaccgca gttaactatg tatataacct
      601 taaatcgaga gaaattaaac ccctttcggt caacggtcca caaatgaatg cggccttctc
      661 gcccgatggc cggatggttg cttttgtcag aaataataac atatacatgg ttaatcttac
      721 cactgccgaa gagtttcagg ttactaccga tggtttatac aacagcataa tcaacggtgc
      781 tgccgattgg gtttacgagg aggaattttc gcttaccacc gggctacaat ggtcgcccga
      841 ctcaagaaaa ctggcatttt accgattcga cgagagtcgg gtgaagatgt tcaacatgac
      901 caagtacgag ggcaaccttt acccttcaaa ctataccttt aaatatccta aggcgggcga
      961 ggagaactcg gtggtttcca ttcatgtttt caaccttgat tctaaatcta ccattaaaat
     1021 ggatgttggg ccagaaacag accagtacat tgcccgaatt aagtggtcag ctgatcctga
     1081 aaaattatgt atggttaggc tcaaccgtct tcaaaataaa gtagatattc ttttggccga
     1141 tgcatcaacc ggcagttcaa gtgtgcttta taccgaaaca aacaagtact acattgaaga
     1201 ggtaaccgac gcctacccga ccttcacccc cgatggtaag tattttatta ttacctccga
     1261 aaaggatggt taccggcaca tttatcttta cggcatgaac ggcagccttg taaaccagct
     1321 tactgcgggc attaacgagg taacagagat gtacggctac agccctttat caaaaatggt
     1381 ttactaccag gcctacgacg attcgcctct ttgtaaggca gtttatgcta agaaaattga
     1441 cggtaaacaa cctgtaaagc tgtcaacaaa tattggcaac aactatgctc agttcagcag
     1501 caatttcgag tactgcatga ttttccactc caacatacaa gaaccgctta ctgtttcact
     1561 gattaacgcc aaaggcaagg aggttagggt ggtagaggat aaccagaagt taagggatag
     1621 gcttaaggag tttgccattc cctcaaagga attttataca ttcacaacca gcgagggggt
     1681 tgagctcaac ggttacatga ttaaaccggt tgattttgac tccacccgga agtaccctgt
     1741 aatgatgtac cagtacagcg gtcccggatc tcaaacagta accaacagct ttagagttgg
     1801 ttgggacgaa tacctggcca ctcttggata ccttgttgtt tgcgtagatg gacgaggaac
     1861 cggtggaagg ggagaagcgt ttaggaagat gacttacggg caaatgggtt actacgaatc
     1921 catcgaccag attgaggctg caaagtacct gcaaaccctt tcatgggttg atgcaggaag
     1981 aattggaatt tggggttgga gctacggtgg atatatgtcg agcctttgcc ttttcaaggg
     2041 agccgatatt tttaaaatgg ccattgctgt tgctccggtt accaactggc gttactacga
     2101 ttccatctat accgaacgat ttatggggct accccaggac aatcctctgg gctacgattc
     2161 caactcgcca attaaccatg ccgataagct tcgagggaaa cttttgctgg tgcatggcac
     2221 tgccgacgac aacgttcact ttcagaacag catggagcta gtcgaacgac ttgtgcaaag
     2281 tggaaaacag ttcgatatga tgatatatcc tgataaaaat catggcatat acgggggtaa
     2341 caccaccatg cacctctaca cgctcttgag caactacgta caagaaaatt tatagtgttc
     2401 ctgctaaatt tccgggtgtc gtgtttggat gaatcagaaa ttgtataatt ttgcatccgc
     2461 attttagccc agatggcgga attggtagac gcgctagttt cagggactag tatcagcaat
     2521 gatgtgcagg ttcgagtcct gttctgggca caaggttcac cccatggtga acctttatta
     2581 caaaaacgcc aaaaatatcg ccctggtgcg gtagaaattt gaaaaaatag gctatttttg
     2641 cattcgttaa ttcccagttg ttattgccca ggtggtggaa ttggtagaca caccagcttg
     2701 aggggctggc gccgtcaaaa gcgtgcaagt tcgagtcttg tcctgggcac caaaccttgt
     2761 aatttttgca aggttttttt atttttgggt aaatcaacat ctaataccat gagaaaagta
     2821 aaacttaccc ttttgctggt ttcggctttc accggctcgt ttgcccaaag cgccgactcg
     2881 ttcaccactc ccaacggcaa gtcgcttcga ataaccttca tcggtcatgg aaccctgatg
     2941 atggagtatg agaacctcgt aattcatgtt gacccatgga gcaagctggc tagctacgac
     3001 aagcttccta aggccgacat agtgctggtt acccaccatc atgccgacca ccttgactca
     3061 gccgcactgg ttaaggtgtg caaagtaggt accgaaattt actggacaca aacctgctcg
     3121 gggagcagta gccttaaacc caaatccacg attatgcaca atggtgacag gcttactaca
     3181 catggtgttg agatttctgc ggttcccgcc tacaacctca ttcacatgac acccggcgga
     3241 actccttttc atgtaaaggg cgaagggaat gggtatatac tgaatttcga cgggtttgta
     3301 gtttatgtag caggcgatac cgaggatatt cctgaaatga agggcttcgg aagggttgat
     3361 atcgcctttt tacccatgaa tcttccttac actatgaccc ctgaaatggt acacaacgca
     3421 gcaaccatgc tacatccaaa gattctttat ccctaccatt atggcgatac cgataccaac
     3481 gccctgttaa acatcatgaa aacaatgccg gaagttgacg tccgtattag gaatttgaaa
     3541 tgatttaaca aaaaagttgt ccggattata ctgtcaggac ctgggacttt ggtggattcg
     3601 atgcatggcg acagcaactt ttgaactccg gacaacttgg ctgctggtgg cagcagtagt
     3661 atattgtttg tgtttgggtt acttctatag tagaaaaatc gtgccaccaa cgccggaacc
     3721 caagctgttt tttataacat actgcatatc tgcaatataa tcttatcctt ccgcatttct
     3781 agctgttgcc tgctgccatt ttgaggaata aaatccccaa attgtagagt tttgaggagt
     3841 ggttgacctg ttgctttcca cactcatgcg tttttacttg acaaaattat accctgttgc
     3901 agatggcgtt atacgggcat attttgcagt tatcggtgtt gtccgtttgg gtaaaatcaa
     3961 cggaggggtt gaaaatttct tccagcaaag ccaccagcat ttgtctaaat ggtgcttgta
     4021 aggattcgaa ggtggtcact tcagtcttag cgctgcttcc cttttttaaa gatttggata
     4081 taaggggaag agtatttttt cgcacatacc aaaggttggg ttgaatgctg ccggcatcaa
     4141 tgccgtactt catgctgtaa atcatacagt aagcaaatag ctggagtatg ccgtcggtgt
     4201 tgctcttgtt ttcaaaaagc tggtctattt ccgaaaaggt taccttgtta ggttcaaact
     4261 taccggtttt aaagtcaatt attcgggtta ggccatttgc ctgttcaacc ctatcaattc
     4321 gtccacctag cttcactaca aaatttcttc catccacaac aataggtata tcaaccattt
     4381 cgctctgctc agaccgggta
//
LOCUS       DAOPHX010000174         4280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_27948_length_4280_cov_5.452308, whole genome shotgun sequence.
ACCESSION   DAOPHX010000174 DAOPHX010000000
VERSION     DAOPHX010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27948_length_4280_cov_5.452308"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..2359
                     /locus_tag="PK502_10050"
     CDS             287..2359
                     /locus_tag="PK502_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPH01200.1"
                     /translation="MLFYACQKNRIQYPETKRVDVVDNYFGTKVPDPYRWLEDENSPE
                     VAEWVSKQNELTSSYLAKIPFREKIRERLTGIWNYTTMGVPFKEGGRYFFFKKDGRQN
                     QSVLYVMETLNSEPRVLLDPNTFSADGTIALSEVSPSRDGKYLAYAVNDGGSDWQKIK
                     VIALPEGKELKDEISWVKFSSIAWYANGFYYSRYDEPKKGEALSTLNQYHKVYYHTMG
                     TDSNEDKLIFANSQHPLRNYSAQTTENEKYLIIYETESTYGNSIYIKNLTDKRGEFSQ
                     LTTGFEYEYSVLENVDDNLYVLTNYKAPKYKLIKININSMDVGNWKDILPERKDVLTS
                     CQLVGGKIIANYVVDAKSKAEVYTLSGEKTDEIHLPTIGTISGFSGSYSDTLAFYSFS
                     SFTVPPAVYKFNVAKNESTEFFKPDIKFNFDDYETKQVFYTSKDGTIVPMFIVHKKGI
                     KLDGSNPTLLYGYGGFNISMMPGFSTSRLFWLENGGVFALANIRGGGEYGENWYRAGT
                     KLNKQNVFDDFIAAAEYLIKEKYTSPKKLVIQGGSNGGLLIGAVINQRPDLFKVAIPE
                     VGVMDMLRFHKFTIGWAWVNDYGSSEDSIQFQNLYSYSPLHNIKPNTKYPAILVTTAD
                     HDDRVVPAHSFKYIATMQEKNPNPQNPALIRIQTRAGHGAGTPTSLIIDEYTDIYAFI
                     LYNLGLSI"
     gene            2534..3163
                     /locus_tag="PK502_10055"
     CDS             2534..3163
                     /locus_tag="PK502_10055"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPH01201.1"
                     /translation="MQYLVIEGNIGAGKTTLATMLANDLNANLITEQFADNPFLPKFY
                     ENPERYSFALELSFLAERYRQLNAELRMGSLFHSLTIADYYFMKSLIFAQNTLSGDEL
                     QLYKQIFQIIYNTLPKPNLYVYLHLPVQMLLRNIKKRGREYEQSITGEYLESIAQGYF
                     DFFKQHPDYTFLIIETSKLDFVANQHDFNKIKSAIFDKTYPNGVNRLTF"
     gene            3274..>4280
                     /locus_tag="PK502_10060"
     CDS             3274..>4280
                     /locus_tag="PK502_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01202.1"
                     /translation="MMKGLKFAIVALAGVVLAGCGGAEKMKELPQGTSFKVTPNPLEV
                     HANKVKATIEGTVPAKYFNKKATLEITPVLVYNGGELALPSKILQGEDVQANNQVINY
                     EAGGSWKHDVEFDYKDEMFRAEMELRFVITYKDKQIPFDAPQKIGIGTMATYKLVDLD
                     PKPVMLKDKFVRVTPEEKEAQINFVIQKSDIRKSELTKDEVKMLNDFLLDAAKTSNKQ
                     LKEIKVSAYASPDGPETLNDKLSKERGKNTDSYLADVFKKGKVTNPATVSVETTSEDW
                     EGFQKQVQASNIEDKDLILRVLSMYSDPEVREKEIKNLSKVYKVLAEKVLPELRRSRM
                     IA"
BASE COUNT         1342 a          935 c          888 g         1115 t
ORIGIN      
        1 aactttatat atcctttaac cgcttcaatt tcttatggaa agcctgattg ggatgaagtt
       61 ttatgccccg ggtagagttc ttgaaagtta acttgaagac aaatctatga gaagctgatg
      121 aatttaatat aattttgggc tagtataaag gtagtcattt taatatccta ctaacactac
      181 aatgtattca ggtagtttga tgccaatacg attacattca aatatgttca actaaattat
      241 tgtatccgtg gaaaaaaaat taatcatact tgctgtaaca atacccatgc tgttttacgc
      301 ttgtcaaaaa aatcggattc agtatcccga aaccaaaagg gttgatgtag tagacaacta
      361 ctttggaact aaggttcccg atccctaccg ctggttggaa gatgaaaatt cgcctgaggt
      421 agccgagtgg gtttcgaagc agaacgagct aacctcatcg tatcttgcta aaattccatt
      481 ccgtgagaag attcgggaaa ggctaaccgg catctggaat tataccacca tgggggttcc
      541 ctttaaggaa ggggggcgat acttcttttt caagaaggat ggacggcaga accagagtgt
      601 gctctacgtt atggaaactc taaactctga accaagagtt ctacttgacc ccaatacctt
      661 tagtgcggat ggtaccatag ccctttcgga agtaagccca tcgcgcgatg gtaaatacct
      721 ggcctatgca gtgaacgacg gaggttctga ctggcaaaag attaaagtga ttgcgttacc
      781 tgaaggaaaa gagctgaaag acgaaatttc ctgggttaag ttctcgtcaa ttgcgtggta
      841 tgccaacggt ttttactaca gccgttacga cgagcctaaa aaaggtgaag cgctctcaac
      901 ccttaaccag taccataaag tttactacca caccatgggg accgattcaa atgaagacaa
      961 gctgattttt gcaaacagtc agcatcccct aaggaactat agcgcccaaa ctaccgagaa
     1021 cgaaaaatac ctgattatct acgaaactga atcgacctac ggtaactcca tctacattaa
     1081 aaacctcacc gacaagcgcg gggaattctc tcaactcaca actggtttcg agtatgagta
     1141 cagcgtgttg gaaaatgttg acgacaacct ttacgttcta accaactaca aagctccaaa
     1201 atacaagctt attaaaatca acattaacag catggatgtt ggaaactgga aagacatcct
     1261 gcctgaacgt aaggatgtgc ttacctcctg ccagctagtg ggtggcaaaa ttatcgctaa
     1321 ctacgtggta gatgccaaga gcaaggccga ggtttacacc ctcagtggtg aaaaaaccga
     1381 tgaaattcac ctaccaacca tcggaacaat ttccggtttc agcggctcct atagcgacac
     1441 gttggccttc tacagcttct cctcgtttac tgttcctccg gctgtttaca agttcaacgt
     1501 tgccaaaaat gaatcaaccg aatttttcaa gcccgatatc aaattcaact ttgatgatta
     1561 cgaaaccaag caggttttct acaccagcaa ggacggtaca attgtgccca tgttcattgt
     1621 tcacaaaaaa ggaatcaaac ttgacggtag caacccaacc cttttatatg gctatggagg
     1681 tttcaacatt agcatgatgc cgggttttag cacatcgaga ttgttctggc tcgaaaatgg
     1741 cggtgttttt gccctagcga atattcgtgg cgggggtgaa tatggtgaaa actggtaccg
     1801 tgccggaaca aaactcaaca agcaaaatgt gtttgacgac tttatagcag ctgctgaata
     1861 ccttattaag gaaaagtata cctcacccaa aaagctggtc atacagggtg ggtcaaatgg
     1921 tggcctcctg ataggagcgg ttattaacca gcgtcccgat ttatttaaag tagccatccc
     1981 ggaagttgga gtgatggata tgctgcgttt tcacaaattt accataggct gggcatgggt
     2041 taacgactat ggatcaagcg aggattccat tcagtttcag aatctctaca gctactcccc
     2101 gctgcacaac attaaaccaa acacaaaata cccagccata ttggtaacaa cggccgacca
     2161 tgacgacagg gtagttccag cgcactcgtt caagtacatt gccaccatgc aggaaaaaaa
     2221 tcccaacccg cagaatcctg cacttatcag aattcaaacc cgtgcaggcc atggtgctgg
     2281 aacaccaaca tccctgatca ttgacgagta caccgacatt tacgctttta tactttacaa
     2341 cctggggcta tccatctagc tcttttatga atgaatactt ttttaaccat gggggttgag
     2401 caacagctta tcccccattc atattgctgt acaacaacgg taaattccga aacatagtga
     2461 caattattga tgacgaattg cggaacagaa tatgctaact tagccaagta gttataaata
     2521 agcaagtagg aatatgcaat accttgtaat agaagggaat attggtgcag ggaaaactac
     2581 cctggccacc atgttggcaa acgatttaaa tgccaatctc attacagagc aatttgccga
     2641 taacccattt ttacctaaat tttacgaaaa tcctgaaagg tatagctttg ctctggagct
     2701 atcgtttctg gccgaacgct acaggcagct gaatgccgag ctacgaatgg gttcattatt
     2761 ccatagtctt accattgccg actactactt tatgaagtcg ctcatttttg cccagaacac
     2821 cctttcgggt gatgaacttc aactctacaa gcaaattttc caaatcatat acaacaccct
     2881 tcccaagcca aatctttacg tttacctgca tcttcccgtt cagatgctac tacgaaatat
     2941 aaaaaagcgc ggcagagagt atgagcaatc cattaccggc gagtacctcg aaagcatagc
     3001 acaggggtac ttcgattttt tcaagcagca ccctgactac acctttctaa tcatcgaaac
     3061 ctcaaagttg gattttgttg caaatcagca cgattttaat aaaattaaaa gtgcaatttt
     3121 tgacaaaacc tatccaaatg gtgttaaccg gcttacattt taggataaat gtatttttag
     3181 ggcatatttt ttatttgtaa aaaaaaattt ctaagtttgt agatgattta ctgaaaaaat
     3241 cgatgttatt aactattaac caaatttgat acaatgatga aaggattaaa atttgcaatc
     3301 gtagcactgg caggagttgt tctggcagga tgcggcggtg ccgaaaagat gaaagaatta
     3361 ccccagggca ccagctttaa ggttacccca aacccacttg aggttcacgc caacaaggta
     3421 aaggccacta ttgagggtac agttcccgca aagtatttca acaaaaaagc cacactggaa
     3481 attacccctg tactagttta taacggtggt gagctggctc ttccttccaa aattcttcag
     3541 ggggaagatg tgcaggccaa caaccaggta attaactacg aagcaggcgg ttcatggaag
     3601 catgatgttg aatttgacta caaggacgaa atgtttcgtg ccgaaatgga gctgcgcttt
     3661 gtaatcacct acaaggacaa gcagattcct tttgatgctc ctcagaaaat tggtattgga
     3721 accatggcaa cctacaagct ggttgaccta gaccccaagc cagttatgct gaaggataaa
     3781 tttgtaaggg ttacccccga ggaaaaagaa gctcagatca acttcgtaat ccaaaagtcg
     3841 gatattcgca aatccgaact taccaaggat gaggttaaaa tgctaaacga ttttctactc
     3901 gatgctgcca aaactagcaa caagcagctt aaagagatta aagtatcagc ctatgcttcg
     3961 cccgatggac cagaaactct taacgataag ctttcgaagg aacgtgggaa gaacaccgat
     4021 tcatacctcg ccgatgtgtt caaaaagggt aaggtaacaa atcccgctac ggtaagcgtt
     4081 gaaaccactt ctgaagactg ggaagggttc cagaagcaag ttcaggcctc gaacattgaa
     4141 gataaggact taattcttcg agttctttcc atgtactccg atcctgaggt tcgcgagaaa
     4201 gaaattaaaa atctatcgaa ggtttacaag gtgctggccg aaaaggtact ccctgaactt
     4261 cgtcgttcga gaatgattgc
//
LOCUS       DAOPHX010000175         4268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_28056_length_4268_cov_5.167102, whole genome shotgun sequence.
ACCESSION   DAOPHX010000175 DAOPHX010000000
VERSION     DAOPHX010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28056_length_4268_cov_5.167102"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1861)
                     /locus_tag="PK502_10065"
     CDS             complement(<1..1861)
                     /locus_tag="PK502_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01203.1"
                     /translation="MKTFTKNQTLPKCLSALLFVLMFTSGMAYSQFSVTIKTLPASDP
                     PTVCQNSSLVLKAYPTGGVTPYGPYTWGGAPFITPAGDIAVLTPDETTTPGIYTISCT
                     VKDHVGNEVTGYIDITVYESPTASITNNGTTTFCQGGSVELEAYSEAGYSFQWRRDDA
                     DISGATSSTYNATVSGSYNVVVTNGNGCDSYSNYIIVTVNPLPSATASNDGPACYNGT
                     INLTSGPNGMVSYSWTSTSTTPFSSTLQNPSITNVTPDNSGDYTVTVTDANGCENSAT
                     TTVLVYANLNGGTIGSDQTICYNGDPLAFTNETSPNGGDGIWTYSWESKVGAGSWTAI
                     GGANGLTYDVPAGLTQTTQYRRVATNGCGTVYSNTITVTVYADLNGGLISSSQSICYN
                     GDPAAFSNDAAASGGDGTFTYSWESKVGAGSWTPILGATGLTYDVPAGLTQTTLYRRK
                     ATNTCGVAYSNELTVTVFGALSGGVVAADQSICYSGDPAAFTNVTSPAGGTGAWTYSW
                     ESKVGAGAWTPIGGANGLTYDVPAGLTQTTMYRRAATNACGTVYSNVLTITVYAQMNG
                     GVISGNQSLCYNSDPSPITSTTNPSGGNGTWSYSWEYQSNCAGVWQSISGATAL"
     gene            complement(2457..3062)
                     /locus_tag="PK502_10070"
     CDS             complement(2457..3062)
                     /locus_tag="PK502_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01204.1"
                     /translation="MTFKPDRWLSILLHPLFMMCYAFLTLYLSASYISFLPDSYKRLL
                     FIALFLNTIIIPIFILMLLKRLGTVKSIELFHHRERVFPIVMVIIPYVFTLLLLIRLQ
                     VPEIFIKIVQGGILAMIIAATVSYWWKISLHMVGIGGVTGFIIAGLILHYFTSLNLLV
                     VVVVLSGLLASARLVRGDHCPSQVYTGYLTGLASILLVFLL"
     gene            complement(3072..>4268)
                     /gene="rpoN"
                     /locus_tag="PK502_10075"
     CDS             complement(3072..>4268)
                     /gene="rpoN"
                     /locus_tag="PK502_10075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPH01205.1"
                     /translation="NYRLVASNVSKDDKREEIPFSVGVTFQESLKSQLGLTSLNDREK
                     QLALYLIGNIDEDGYLRRKLSAIADDVAFALGLDTNEDELEKILYVIQEFDPPGVGAR
                     DLQECLLLQLRVKENGSTTASTAYKILKYHFDEFTRKHYDKIAQKLSLSDEQLRDAIN
                     EILKLNPKPGGGFSDPYNQSIQQVIPDFILDYVDGEFQLNLNSRNVPELRISREYSDM
                     LESYSYSKDSATSQEKEAVTFVRQKIESARWFIDALKQRQNTLMTTMRAILNYQQEFF
                     RDGDESKLKPMILKNIADATGLDISTISRVANSKYIQTHFGIYPLKYFFSEGLMNDAG
                     EEVSTREIKKILQECILNEDKGNPLTDEELMEALKQKGYPIARRTIAKYREQLGIPVA
                     RLRKQL"
BASE COUNT         1120 a          943 c         1028 g         1177 t
ORIGIN      
        1 taagcgctgt tgcgcccgaa atggactgcc atacccccgc acagttggat tgatactccc
       61 atgagtacga ccatgttccg ttacctcccg atggattggt agtgctggtt attgggcttg
      121 ggtcggaatt atagcaaagc gattgattac cggaaattac gcccccgttc atttgtgcgt
      181 aaacggtaat tgttagcacg ttggagtaaa ctgttccaca agcattagtg gctgccctcc
      241 tgtacatggt ggtttgagtt aaaccggcag gaacatcata ggttaaacca ttggctccgc
      301 caataggtgt ccatgcgcca gcaccaacct tcgactccca gctgtaggtc catgcgcctg
      361 ttcccccggc aggcgaggta acattggtaa acgcagcagg atcgccactg tagcaaatac
      421 tctggtctgc agccacaact ccaccgctta atgcgccaaa taccgttacc gttagttcgt
      481 tggagtaagc cactccgcag gtattggttg ccttacgcct gtacagggtt gtttgtgtaa
      541 gccctgcagg gacatcatag gttaagcctg ttgcgccgag aattggtgtc catgagccag
      601 caccaacctt cgattcccat gagtaggtaa aggtaccgtc tccacccgaa gcagcggcat
      661 cgttgctaaa tgctgcggga tcgccgttgt agcagatgga ctggctggag cttatcaaac
      721 caccgtttaa atcagcgtaa acagtaacgg ttatggtgtt ggagtaaacc gttccacaac
      781 cgttggttgc aacccgccgg tactgggtgg tttgcgtcaa tccggcaggc acgtcgtacg
      841 ttaggccatt ggctccgcca attgcagtcc atgagccagc gccaaccttc gactcccagc
      901 tgtatgtcca aatcccatcg ccaccattag gcgaagtttc atttgtaaat gcaaggggat
      961 cgccgttgta gcaaatggtt tggtcggagc caattgttcc gccattaagg ttggcgtaaa
     1021 ccaaaacggt tgttgtggca gagttctcac acccattagc atcagtaact gttacggtgt
     1081 aatcgccgga gttatcggga gtaacgttgg ttatgcttgg gttctgaagg gttgacgaaa
     1141 agggggttgt tgatgtgctt gtccagctgt acgaaaccat accattgggg cctgaggtca
     1201 ggttgatggt accattgtag caggcaggtc cgtcgttgga ggctgtggca ctcggaagag
     1261 ggttgaccgt tacgatgatg tagtttgagt agctgtcgca tccatttcca ttggttacca
     1321 ccacgttgta tgatcctgat acagtagcgt tataggttga tgaggtagct ccggatatat
     1381 cagcatcatc cctgcgccat tggaaggagt aacccgcctc gctgtatgcc tccagctcaa
     1441 ctgagcctcc ctggcaaaaa gtagttgttc cgttatttgt aatgcttgca gttggggatt
     1501 cgtaaactgt aatatcaata taccctgtaa cctcgttgcc cacgtgatct tttacagtgc
     1561 atgaaatggt ataaataccg ggagtagtag tttcatcagg agtaagaact gcaatatcgc
     1621 ctgcaggggt aataaacgga gccccgcccc atgtgtaggg tccataagga gttacccctc
     1681 cagtaggata ggcctttaat accagcgaag agttctggca gactgttggg ggatcgcttg
     1741 ctgggagggt ttttatcgta actgaaaact gcgagtatgc cataccgctg gtaaacatca
     1801 gcacaaaaag taatgccgat aagcatttgg gtagggtttg gttcttggta aaagttttca
     1861 tcctgtagat atttgggtgt aaattatgta acaataaaga tagtcgctcc cacatacgaa
     1921 ctattggttt tgttgctaat taaataagct gccatttcca gcctagcatg ggttaaccgg
     1981 tttgctatta acaacagcag gccaactagt taatttttac gaaacaccag gtcaaaatgt
     2041 taatatcttg ccttaaaatt gttgaaaact taaatgctac aagcttgcaa cttagaaaat
     2101 ttctataatg aataacgaaa gtagagctat tttgctaatt tgcaaacctc tatgggcata
     2161 aaaaaattaa taatatttat ccatctattg ctacaagact gattttctgt gtgttaaaat
     2221 ttatagggca aagttgaata atttttgggt atgctgaact ttcaacacca taaaacctga
     2281 tcggcatgtt taagaaatta ttacgagcca tctactttgc agcaggttta ttgagcttat
     2341 aattagttgt actacaaacc gcttaccata aataataact tactgtttat caggcaacta
     2401 tattataatt tggataacac ccgtgcccga aatcggttac tcgtcgggca tggctattaa
     2461 agcaagaata ccagcaaaat agaggccaat ccggtaaggt agcctgtata aacctgtgaa
     2521 ggacaatgat cacctcttac cagcctggcg ctggctaata aacccgatag aactacaacc
     2581 acaactagca gatttaggga ggtgaagtag tggagaatca gcccggcaat tataaatccg
     2641 gtaacgcctc caatacctac catgtgaagg cttattttcc accagtagga tacggttgct
     2701 gcaataatca tggccagaat gcctccctga acaattttga taaagatttc cggaacctga
     2761 aggcggataa gcagcaacag cgtaaaaacg taaggaatta tgaccatgac aatgggaaaa
     2821 acccgctcgc ggtggtggaa aagttcaatg cttttgaccg tcccgaggcg tttcaacagc
     2881 atcagaatga aaatggggat aatgatagta tttaaaaata gggcaataaa gagtaacctt
     2941 ttgtatgaat ccggtagaaa tgagatatat gatgcggata ggtacagcgt taagaaggcg
     3001 tagcacatca tgaaaagcgg gtggagcaga atggaaagcc aacggtcggg tttaaaagtc
     3061 atgtttaagg tttaaagttg ttttcttagt cgggcaaccg gaatgcccag ctgttccctg
     3121 tactttgcaa ttgtccggcg tgcaatggga taaccttttt gctttaaggc ctccatcagc
     3181 tcctcgtcgg tgaggggatt gcctttatcc tcattaagga tgcactcctg aagtattttc
     3241 ttaatttctc gggttgaaac ctcttcgccc gcatcgttca taaggccttc gctaaaaaag
     3301 tatttcaggg gataaatacc gaaatgggtt tggatatact tactgttggc aactcgtgat
     3361 attgtggaaa tgtcgagtcc ggtggcatcg gcaatatttt tcagaatcat gggtttcagc
     3421 ttcgattcgt ccccatcacg aaaaaattcc tgctggtagt taagtattgc cctcatcgtg
     3481 gtcatcaggg tgttttgccg ctgcttaagg gcatctataa accagcgtgc cgattcgatt
     3541 ttttgcctta caaaagtcac agcctccttc tcctggctgg ttgcgctatc cttgctatag
     3601 ctatacgatt caagcatgtc ggaatactcg cggctgattc ttaactcggg aacatttcgg
     3661 gagttgaggt taagctgaaa ttccccgtca acatagtcga ggataaaatc ggggataacc
     3721 tgctgaattg actggttgta aggatcgcta aatcctcctc cgggttttgg gtttagctta
     3781 agtatttcgt tgatggcatc gcgaagctgt tcgtcggaga gtgaaagctt ctgcgcaatt
     3841 ttatcataat gctttcgggt aaattcgtca aaatggtact ttaaaatttt atatgcagta
     3901 gatgcagtgg tgcttccatt ttcctttacc ctgagctgga gcagcaggca ctcctggagg
     3961 tcacgggctc caactcccgg tgggtcaaac tcctgaataa cgtaaagaat tttctccagc
     4021 tcgtcctcgt tggtatcgag gcctagggca aaggcaacat catcggcaat ggccgacaat
     4081 ttcctgcgca ggtagccgtc ctcgtcgatg ttgccaatca ggtaaagagc cagctgcttc
     4141 tcacggtcgt taaggcttgt taaccccagc tggcttttta ggctctcctg aaatgttacc
     4201 cccaccgaga acgggatttc ctccctttta tcatccttcg acacattgct ggcaactagc
     4261 cggtagtt
//
LOCUS       DAOPHX010000176         4212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_28577_length_4212_cov_6.740197, whole genome shotgun sequence.
ACCESSION   DAOPHX010000176 DAOPHX010000000
VERSION     DAOPHX010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28577_length_4212_cov_6.740197"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(306..1049)
                     /locus_tag="PK502_10080"
     CDS             complement(306..1049)
                     /locus_tag="PK502_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HPH01206.1"
                     /translation="MNIKLKIWRQKNAQSKGGFETYLLDGVSPEMSFLEMLDYLNSRL
                     IIEGKSPVAFDFDCREGICGSCGIYINGRPHGSDRKTTTCELRMRKFKDGETITLEPW
                     RAGPFPVIKDLMVDRSAFDKIIQAGGYITVNAGAAPEANSILVPKKNADTAFDTASCI
                     GCGACVAACKNASAMLFVAAKITHLAQFPQGRIEAPERVKRMVAKMEELGFGGCTNTR
                     ACEAECPKGISITAIAKLNRDYISAKMKD"
     gene            complement(1083..3002)
                     /locus_tag="PK502_10085"
     CDS             complement(1083..3002)
                     /locus_tag="PK502_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HPH01207.1"
                     /translation="MTKLDAKIPEGPLADKWTSHKAKLRLVNPANRKKIDIIVVGTGL
                     AGSSAASTLGELGYNVKVFCYQDSPRRAHSVAAQGGINAAKNYKNDNDSVYRLFYDMI
                     KGGDYRAREANVYRAAEVSNLVIDHYTALGVPFARDYGGHIDNRSFGGVQVSRTFYAK
                     GQSGQQCLIGAYSDLSRQISMGTVTMYPRREMMDLVLVDGKARGIVVRNLITGEIERH
                     SAHAVVLATGGYGTIYYLSTLALNSNGSAAWKAYKKGAYFANPSFVQIHPTSIPVLND
                     YQCKLTLMSESLRNDGRVWVPKQKGDTRKPNDIPEEDRDYYLERRYPAFGNLVPRDVA
                     SRAAKERCDAGYGVGPTGLSVYLDFRDAISRKGKKYIEDNYGNLFEMYEKISGESPYS
                     VPMRIYPAGHYTMGGLWVDYNLMTTVPGLFAIGESNFSDHGANRLGASSLMQTSGDGY
                     FILPYTISDYLADEIRTPKISINIPEFDEAENTVRERINKLLGINGTETSTSFHKRLG
                     KIMWDYCGMVRNEEGLKKALALIDELKDEFWHNLKLPDSSNEVNHELEKACRVADFLE
                     LSKLLVYDALNRRESCGAHFREESQTETGEALRDDEHFSYVAAWEFAGDSKEPILHKE
                     ELKFEYVKLQERSYK"
     gene            complement(3025..3696)
                     /locus_tag="PK502_10090"
     CDS             complement(3025..3696)
                     /locus_tag="PK502_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HPH01208.1"
                     /translation="MMGIFSSSIGKKLIMSITGIFLMIFLLVHLSVNLTLLVGDGETF
                     NHAAHFMGTNPLVKIIEPILAAGFILHIFFATYITLKNRSTRPVGYTQTSRNGVSTWP
                     SKNMYILGGLILVFLVIHLSNFFWKMRYGEMPTITYNGLVMDDAYSLVADLFIKYWWY
                     DLLYLAAAVLLGLHLSHGFWAAFQTVGWNGTKWLKRLEVIAYIYAVIIGIGFSVIPLY
                     FLIFK"
BASE COUNT         1176 a          995 c          868 g         1173 t
ORIGIN      
        1 cctcagcttg ggaagctgat gctctaccaa ctgagctact cccgcaacga actgcactcc
       61 tcagcttgcg atccgccggc tggcggacta ccgtctgagc tactcccgca acgaactgcg
      121 ctcctcagct tgcgatccgc cggctggcgg actaccagct gagctaatcc cgcaaatatg
      181 aaagtttaaa gcctgataat ttgtcttaat aattactgaa attctgcgca aaggtaattt
      241 tttatcgcat ttcatcaacc tattttttct taatagttta ccataagccc aaaataccaa
      301 taaaatcaat ctttcatttt ggctgaaatg taatccctgt ttagcttggc aatggcagta
      361 atagagattc ctttggggca ttctgcttca catgcacgag tatttgtgca gccgccaaag
      421 cccagctctt ccatttttgc aaccattcgc tttacccttt caggagcctc aatcctaccc
      481 tgaggaaact gggctaaatg agtaatctta gcagcaacaa acagcatggc tgaagcattc
      541 ttacaggctg caacacaagc cccgcatcca atacatgagg cggtatcaaa agcagtatct
      601 gcgttctttt tgggaactag tatgctgttt gcctctggcg cagcaccagc gttaacggtt
      661 atatacccgc cggcctgaat aattttgtcg aatgctgacc tgtcaaccat caggtctttg
      721 ataaccggaa aaggtcctgc acgccaaggt tcaagggtta ttgtttcacc atccttgaat
      781 tttcgcatac gcaactcgca agttgtggtt tttctgtcgc tcccgtgggg ccttccgtta
      841 atatatattc cgcaagaacc acaaatgccc tccctacagt caaaatcgaa tgcaacaggg
      901 cttttaccct ctataattaa gcggctattc aagtagtcta acatctccaa aaatgacatt
      961 tcaggagaaa caccgtcaag caggtacgtt tcaaaacctc ccttgctttg ggcatttttt
     1021 tgcctccata ttttaagctt gatattcatt tgtcttaata gttaaaacaa cgtaaatatt
     1081 tactacttat agcttcgttc ctgaagttta acgtactcga atttcaactc ctccttgtgg
     1141 agaattggct ctttactgtc gcccgcgaac tcccaagcag cgacatacga aaaatgctca
     1201 tcatcgcgca aggcctcgcc cgtttcggtt tgactttcct ccctgaagtg tgcaccacaa
     1261 ctttccctac ggttaagcgc atcgtaaact agcagttttg acaactccag aaaatcggcc
     1321 accctacatg ccttttcaag ttcgtggttg acctcattgg aagaatcagg tagtttaagg
     1381 ttatgccaaa attcatcttt tagctcatca attaatgcta aggctttttt taaaccctcc
     1441 tcattacgaa ccattccaca gtagtcccac ataatcttac ccaggcgctt atggaacgaa
     1501 gtggatgttt cggttccatt tataccaagc agcttgttaa tgcgttcacg aacagtattt
     1561 tccgcctcgt cgaactcagg aatgttaatt gaaattttgg gagtacgaat ttcatcggca
     1621 aggtagtcgg aaatggtata aggaagaata aagtacccgt cgccagaggt ctgcatcaag
     1681 gaacttgcac cgaggcggtt tgctccgtga tcggaaaagt tggattcgcc aatggcgaac
     1741 aagccgggca cggttgtcat caggttataa tcaacccaaa gcccccccat ggtgtagtgg
     1801 cctgccgggt aaattcgcat gggaacagag tatggcgact ctcctgaaat tttttcatac
     1861 atttcgaaga ggttaccata gttatcctcg atgtacttct tccctttccg gctaatggca
     1921 tcacgaaaat caaggtaaac agacaaaccg gtaggaccaa caccataccc cgcatcgcag
     1981 cgctcctttg cggcacgcga agctacatcc ctaggaacca gattgccaaa agccggatac
     2041 cgacgttcca ggtagtaatc cctatcttcc tcaggaatat cgttaggttt gcgggtgtcg
     2101 cctttttgct tcggaaccca aactctacca tcgttgcgga gcgattccga cattaacgtt
     2161 agcttgcact ggtagtcgtt cagcacagga atgctggttg gatgaatttg aacaaaactg
     2221 gggttagcaa aatacgcacc ctttttgtaa gctttccaag cggcagaacc atttgaattt
     2281 agcgctaatg ttgataggta gtagattgtg ccatatccac cggtggccaa aacaacggca
     2341 tgagcggaat ggcgctcaat ttcgccggtg ataagattgc gaacaacaat acctcgcgcc
     2401 ttgccatcaa ctaaaaccaa atccatcatc tcgcggcgag gatacattgt aactgtgccc
     2461 atgctaatct gccgcgatag gtcagagtaa gcgccaatca aacattgttg accactttgc
     2521 cctttggcgt agaatgttct ggacacctgg acgcccccaa atgaacgatt atctatatgt
     2581 ccaccatagt cccttgcaaa cggaactccc agcgcagtat aatggtcaat taccaggtta
     2641 ctaacctctg cggcacggta aacattggcc tcgcgggcac ggtaatcgcc tcccttaatc
     2701 atatcgtaga aaagacgata aacgctatcg ttatcgttct tgtaattttt tgcagcgttt
     2761 ataccaccct gagcagccac agagtgtgct ctgcgtgggc tatcctgata gcagaaaact
     2821 ttaacgttgt agcccaactc gcccagagtt gaggctgccg atgagccagc caacccagtt
     2881 ccaacaacga taatatcaat cttcttacgg tttgcggggt tcacaaggcg taacttcgcc
     2941 ttatgacttg tccatttatc ggccagtgga ccttcaggta tttttgcatc gagcttagtc
     3001 atatatagta tatgttagct gtgattactt gaaaattaag aagtagagag gaattacgct
     3061 aaaacctata ccgattataa ccgcatagat ataggcaatc acctctaacc gcttaagcca
     3121 tttagttcca ttccagccta cggtttggaa agcagcccaa aaaccgtggc taaggtgcaa
     3181 ccctaggagt actgctgccg ctaagtaaag caagtcgtac caccagtatt ttataaaaag
     3241 atcggccaca agggagtaag catcatccat taccagaccg ttataggtaa ttgtgggcat
     3301 ttcgccatat cgcatcttcc agaaaaagtt ggaaaggtga ataaccagga atactaaaat
     3361 caacccaccc aaaatgtaca tattttttga tggccatgtg gaaactccat tccttgaagt
     3421 ttgggtataa ccaaccggac gggtgctcct gttctttaat gtgatgtaag tagcaaagaa
     3481 gatgtggaga ataaaacctg cggccagaat tggctcgata attttgacta aagggttggt
     3541 gcccataaag tgggcagcat ggttaaatgt ttctccgtct cctacaagta gcgtcaggtt
     3601 tacggagagg tgaacaagca ggaatatcat caggaaaatc cctgtaatac tcattatcaa
     3661 ctttttccca atggacgagc tgaaaattcc catcatggca ttaaagttta attttggtga
     3721 acaagttaga aaaagttctt aaataataaa atacatggcc gtacgcaaaa gcttataaat
     3781 ttgaaatttc agatatcacc gatttaccct tcccaatggc cagcgctata gttttttggc
     3841 tgcttccctg ttgcatcacg gcctaaatgg tgtacctttg cgctacggtt aagtgcttca
     3901 tctttgtaac tttttgaggg aagggagcaa agtaaggcat ttactccgtc aggacaaggg
     3961 gggaaaaaat gcttttttct cctttgtttc ttgaaaaagc ttgttttttt acttccctga
     4021 ctcccttaaa aagttgcatt aattaattac tttaatttag gtaagaataa ttatgtaacc
     4081 tttttaattc tagaaaaaat gaataaaata aaagagatgt tctgtttaat aagcctttct
     4141 ttgttagtaa gtaatgcaac aggccaaggg ctgtaccaaa aatcttggtt ggtagatgtt
     4201 agtgcaggaa cc
//
LOCUS       DAOPHX010000177         4203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_28652_length_4203_cov_6.597396, whole genome shotgun sequence.
ACCESSION   DAOPHX010000177 DAOPHX010000000
VERSION     DAOPHX010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28652_length_4203_cov_6.597396"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..365
                     /locus_tag="PK502_10095"
     CDS             42..365
                     /locus_tag="PK502_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPH01209.1"
                     /translation="MEVENAKAQMRKGILELCILSVLSKGDAYANDLITKLKEAKMIV
                     VEGTLYPLLTRQKNLGLLSYRWEESPQGPPRKYYSLTDKGRTYLNELHRSWDELVETV
                     NTLRN"
     gene            600..2165
                     /locus_tag="PK502_10100"
     CDS             600..2165
                     /locus_tag="PK502_10100"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPH01210.1"
                     /translation="MKKTVRVSLSGFSYNLEEDAYRLLDHYLEEIRVRLGSNADAEET
                     LRDIEERISELLTARMGQRETVPADMVQDIINQIGMPEEVTGNAEYSKDQWETQPPKK
                     RLYRDTENPVIAGVCSGVAAFFGVDPIVIRIIFIALLFARGFGLLLYIILWIVVPRAL
                     TPKQKLEMRGQPVTLSNMGKNISEEMSSVKSSVSQKVPRNFFERLGKFLGQVGFWLLK
                     FIMVLVKVVAIAIGIVLIVSMLFAFIVLVGAFFFSGITLPWLFPGTVVSSLNEFITSM
                     IDISSGLWITIPTFLILAIPIVALIYIGIRVLFRFKARDGKIAIIATGIWVASVLTLA
                     ISIFIQLRSLSFSGADRQVVQLSSQLPRNQQTIYLKAFPQYDEATLPSVYKFFDYSIT
                     TVQGEKVISGQPKLVIEKSDSDSISLILSKNARGFSSTNAAKNAADIIYPYSVKDSTI
                     FVDSRFTLPASVTWKGQTLTLSLLLPEGYSIYLDSSICGILDTDQPYSSHWPDEMVGQ
                     TWTMTRNGLRVKR"
     gene            2355..2425
                     /locus_tag="PK502_10105"
     tRNA            2355..2425
                     /locus_tag="PK502_10105"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2387..2389,aa:Gln,seq:ttg)
     gene            complement(2488..3336)
                     /gene="nfo"
                     /locus_tag="PK502_10110"
     CDS             complement(2488..3336)
                     /gene="nfo"
                     /locus_tag="PK502_10110"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HPH01211.1"
                     /translation="MKFVGAHVSAAGGVENAPVNANAIGATAFALFTKNQRQWVAPPL
                     SPKSIDLFRRRCEEFSYSPAQVLPHDSYLINLGNPDRDALEKSRNAFTDEMARCELLG
                     LDRLNFHPGSHLNKTTPEECLNLIAESINMALDKTHGVTAVIENTSGQGSNLGFTFEQ
                     LRYIIDRVDDKTRIGVCIDTCHAFTAGYDLLTPEAFTKTFSEFDRIIGFGYLRGVHLN
                     DSKKGLGSRVDRHENIGLGLLGAEPFRMLMGDPRFDGIPLILETPDESKWPEEIALLK
                     GFDREI"
     gene            3586..4032
                     /locus_tag="PK502_10115"
     CDS             3586..4032
                     /locus_tag="PK502_10115"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPH01212.1"
                     /translation="MPAIRLTKEFRFEMAHVLEGYDGPCRNIHGHSYKLKVTVIGNPI
                     SQPGHPKLGMVIDFGDLKQVVETLITSRFDHSIAVLENSNIHRKLAGSDLEKVEALPF
                     QPTCENMVEYFARLLAPSLPEGVSLHHLLLHETATSYAEWYADDNP"
BASE COUNT         1136 a         1006 c          959 g         1102 t
ORIGIN      
        1 tattggtaaa tcgtttgaaa acaaaaaata accctacgga tatggaagtc gaaaatgcca
       61 aggcgcagat gcgcaaagga atactggagc tatgcatact ctcggtgctt tcaaaaggcg
      121 atgcctacgc caacgatctt ataaccaagt taaaagaagc caaaatgatt gtggttgagg
      181 gaaccctcta tccactgctt acccggcaga aaaacttggg attacttagc taccgctggg
      241 aggaatcgcc acaggggcca ccgcgcaagt actactccct caccgacaag gggagaactt
      301 acctaaacga gctgcatcgc agctgggacg agctggtgga gacagtgaat acacttcgaa
      361 actaatagct acaagaaata cctgccgtaa cttttaacct taacctatga tgctgaactt
      421 caacacgaca cttaatcgtc catactttcg ccagcatgtc ataccgacga taaaaagtag
      481 ccgtaattca gctttcgttt aggtgaggtg aggcagaagt ttagcccaag gatggtttaa
      541 ccaaccattt taccataaaa acactacttt gttgccaacc gtaaagtaaa ttaaagcaaa
      601 tgaaaaaaac agtaagagtg agcctttcgg gcttttcgta taacctggaa gaagatgcat
      661 acaggctgct cgaccattat cttgaagaaa tcagggtgag gttgggttca aatgctgacg
      721 ccgaagaaac cttaagggat attgaggagc ggatttccga gctgcttact gcccgaatgg
      781 gccagcgtga gactgttccg gccgacatgg tgcaggatat tatcaaccag attggtatgc
      841 ccgaggaagt taccggaaat gctgaataca gtaaagatca gtgggagact cagccaccca
      901 aaaagaggct ttaccgtgat actgaaaacc ctgtaattgc aggtgtttgc tcgggcgttg
      961 ctgccttctt cggggttgat ccaattgtaa tcagaatcat ttttattgcg ctactgtttg
     1021 ccagagggtt tgggctgctc ctgtacatca ttctctggat tgttgttccc agagcgctaa
     1081 cacctaagca gaaactcgaa atgaggggac aaccggttac gctgtcgaat atgggcaaaa
     1141 atatttccga ggagatgagc agcgttaaga gctccgttag ccaaaaggtt ccaaggaact
     1201 tttttgaacg tcttggaaag tttctgggtc aggtaggctt ctggctgttg aaatttatca
     1261 tggttctcgt aaaggttgta gctattgcca taggcattgt gctgatagtt tccatgctct
     1321 ttgctttcat tgtactggtg ggagcattct tctttagcgg aattaccctg ccctggctgt
     1381 tccccggaac agttgtaagc tcgctaaacg agtttatcac ctcgatgatt gacataagta
     1441 gcggcctatg gatcactatt cccaccttcc ttattctagc cattcccatt gtcgccctaa
     1501 tctacatagg cattcgggtt ttgttcaggt tcaaggcacg tgacgggaaa attgcgatta
     1561 ttgcaacagg gatctgggtc gcctccgtgc taacccttgc catcagcatt ttcattcaac
     1621 tacgaagcct aagtttttcc ggagcagaca ggcaggttgt gcagctgagc agccagctgc
     1681 ccagaaacca acaaaccatc tacctgaaag cattcccgca atacgatgaa gcaacgctac
     1741 cctcggtata taaattcttc gactactcca taacaacagt acaaggggaa aaggtaattt
     1801 ccgggcaacc gaagctggtt attgaaaaat cggattccga tagtatcagc ctaattttat
     1861 caaaaaatgc acgcggattc agcagcacaa atgcagctaa aaatgcagcg gatatcattt
     1921 acccatactc ggtgaaagat tccaccatat ttgttgacag ccggtttact ctaccagctt
     1981 cggttacgtg gaagggtcaa acactaactt tatccctttt acttccggaa ggatacagca
     2041 tttaccttga ctctagcatt tgcggcatcc tggacaccga ccaaccctac agcagccact
     2101 ggcccgatga aatggtcggg caaacctgga ctatgacccg caatggatta cgtgtaaaaa
     2161 gataggtagc aattgcccat acgatgagtt gttgcttaat tgttcctggt gaaggctgcg
     2221 tgtattagat acaacaatgt tatgctatgc gcaaagacta cctttgccca taatgagatg
     2281 gcgaatgaat agccaaaatt ttttggcggg tgataaaaaa tccattactt ttgccccact
     2341 ataactaggg gtattgacct atggtgtaat ggtagcacag cagattttgg ttctgttagt
     2401 ctaggttcga atcctggtag gtcaacttta aaagcattcc tatgaaaacg gaatgctttt
     2461 atttttaagc cgagctggag gcaatcctca aatttccctg tcaaagccct tgagcagggc
     2521 tatttcctcg ggccacttcg attcgtcggg agtttcgagg ataagcggaa taccgtcgaa
     2581 acggggatcg cccattagca ttcggaatgg ttcagcacct agcaaaccaa gaccaatatt
     2641 ctcatggcgg tcaaccctgc ttccaagccc ctttttggaa tcgtttaggt gaactcccct
     2701 caagtagcca aacccgataa tcctatcaaa ttcagaaaat gttttggtaa atgcctcggg
     2761 ggtaagcagg tcgtatcctg ccgtaaatgc gtggcaggta tcaatacaaa cgccaatgcg
     2821 ggttttgtca tcaaccctgt cgatgatgta ccgcagctgt tcgaaggtga accccaggtt
     2881 gctcccctgc cccgatgtat tctcgataac cgccgtaacg ccgtgcgttt tatccagtgc
     2941 catgttgata gactcggcaa tcaggttgag gcattcctcc ggggtagtct tatttaggtg
     3001 gcttcctggg tggaagttga gcctgtcaag acccagcagc tcacaccttg ccatctcgtc
     3061 agtaaaggca tttctggatt tctctagcgc atccctgtcg gggtttccta aatttatcaa
     3121 gtagctatcg tggggtaaaa cctgtgcagg gctgtagcta aactcctcac aacgcctacg
     3181 aaacaaatct atgctcttgg gcgataaggg gggggcaacc cattgccttt ggtttttggt
     3241 aaacagcgca aaggcagtag ctccaatggc gttggcgttt accggtgcat tttccacccc
     3301 gcctgctgcg ctaacgtgtg ctcctacaaa tttcatatcc tgatggattt taacataagc
     3361 aatctgaaga aatgccttta ccaaatacct gttttctccc ccaccctaaa aacctcaggt
     3421 tgattaccat ttccatggga agcttgctgt catcggacta aaggccagaa catcctgatc
     3481 atcaaaagtc gaaaatatct gacaatttaa caaacttcgt cgacgaacgt tcactatatt
     3541 tgtaaaaatg attaccgtaa tgcggtaacg gctaactttt ctacaatgcc agcaattcgt
     3601 ctaaccaaag agtttcgctt tgagatggcc catgttcttg agggttacga tggaccatgc
     3661 cggaacatac atgggcactc ctataagcta aaagtaacag taatcggcaa ccccatcagc
     3721 caacccggac accccaagct ggggatggtt atagactttg gcgatctgaa gcaagttgtt
     3781 gaaaccctca ttaccagccg tttcgaccat tcaattgcgg ttttggaaaa ttccaacatt
     3841 cataggaagc tcgctggctc cgacctcgag aaagttgagg ctttaccttt tcagcccacc
     3901 tgcgagaaca tggtggagta ttttgcccgc cttctggcac ccagcctacc cgaaggcgtt
     3961 tcccttcacc acctcttgct gcacgaaacg gctacctctt acgccgaatg gtatgccgat
     4021 gataaccctt gaaccgcttc cggtgaaaaa agcatttcgt ttgtttcatc tgttctgtta
     4081 gggcaagcag caagcttgct ccaagtgtcc tattaggtta acgctataac taaattcctc
     4141 gcccgacgag gttgggcgta gtattgcgaa gcacacctgc gtccagtttt tcaggcagtg
     4201 cct
//
LOCUS       DAOPHX010000178         4171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_28956_length_4171_cov_4.699708, whole genome shotgun sequence.
ACCESSION   DAOPHX010000178 DAOPHX010000000
VERSION     DAOPHX010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28956_length_4171_cov_4.699708"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2007)
                     /locus_tag="PK502_10120"
     CDS             complement(<1..2007)
                     /locus_tag="PK502_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPH01213.1"
                     /translation="MRRINRALLPVALLSLTCGVGLQAQTLQEAITLTKNEQYDKAEA
                     MFQQLIKNEPGNSKVYFFYGENVLLDYFADTISNSLNVATKKAADIFNNGITASPNEP
                     LNYVGLARVSFYNRDDAKAEEFRLKAKSLLPPYKKVSKIPNPKDYAFTLAKIGESYIR
                     FGQVDTSKAMPYIRQALQIDSRNSEVYIIAGDLYILVNDGSKAIKNYNLAQDWDPTSP
                     TANMKIGSIYVKGRNLMAAIPFYEQAIALDQNYAPAYRELGQLYSMAGRFNESKKYFE
                     KYLELTKGNIPAKIRYVNALFYAKEYKEVIKNVEEIFAIDDSRAYLNRIAGYSAFEEG
                     NYELAKNYMDKLFASLDPERLIKKDYLYYARILSRKNQNLGKLTIEADNLEADLTKLQ
                     TKYASAKPGPAKDKAKIEIDALKAKLDSVKALVERSEAEITLALEAYDKAALFDGEED
                     LNITYEKGTVQYAAKRYADAAQTWMHLIDLGRNSENDYIQVGKAFYQAKEFDKADSVF
                     NAMVTKYPDNLQGYLWMANIASAKDPDSELGLAKPKFQLVLQKAAVDSVKYVKEMYDA
                     LRYLGYEALQNKRYDEAKAYYYRMMNLSPDYVIRAHSSLSTMYISMGDYANAIAENNK
                     ILAIDPNNEVAKSSIQYITQLQKSALPKASPNEISGVITDSAGEP"
     gene            complement(2029..2991)
                     /locus_tag="PK502_10125"
     CDS             complement(2029..2991)
                     /locus_tag="PK502_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPH01214.1"
                     /translation="MGKISTFKQVVQLAILAVAIVAVLPACKNKGAKVPAETPTSGSI
                     KIEADESFKPIVDAEVSTFTSLYNLAHIQTVYKPERELIADFLNDSVQVIVSSWTPTE
                     EQRKTLLDAQTVVRTTLVAYDALAIVLNKGNNDSLLTYDNVKDIFTGKITDWNQLNTR
                     SKLGKISIIFDNIHSGNIRYFREKFNLPQQLPPNFYAVNSNAEVVNYVAKSPGALGIV
                     SVNWISDTDDSTSFDLGSKVKLAAISQQYFDKDSYYLPVQGSIYQKTYPFIREVNINS
                     RESFYGLGSGFISFVAGEKGQRIILKSGLVPATMPIRIVEVKNE"
     gene            complement(3310..3543)
                     /locus_tag="PK502_10130"
     CDS             complement(3310..3543)
                     /locus_tag="PK502_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01215.1"
                     /translation="MSTGKMYDKVFAIFGVFMVFFYIGLGVFIIFHPLLDHLDKALRL
                     IFGITLIIYGIYRIMYSYEKIKKVFFSKDDDDE"
     gene            3909..>4171
                     /locus_tag="PK502_10135"
     CDS             3909..>4171
                     /locus_tag="PK502_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01216.1"
                     /translation="MKYLHITQLIVFTCLCTTLHAQHPERYFRFVEPSKETINTKITR
                     IISIDRVKADTVWAYANDEEFARFSELGYKVELLTPPSLLPGKV"
BASE COUNT         1086 a          908 c          912 g         1265 t
ORIGIN      
        1 aggctcacca gcagaatcgg taataacacc cgatatctcg ttaggactgg ctttgggcag
       61 agcgctcttt tggagctgtg taatgtattg tatggacgat ttggctacct cgttgttggg
      121 gtcaatggcc agaattttat tattctcggc aatagcgttg gcataatcac ccatggagat
      181 gtacatggtg ctgagggagc tgtgtgctct gataacgtaa tcgggcgaaa ggttcatcat
      241 ccgatagtag tatgccttgg cctcgtcgta gcgtttgttc tgcagagctt cgtatccgag
      301 gtagcggagc gcatcgtaca tttccttaac atatttaacg gagtcaacgg cagctttctg
      361 caaaacaagc tggaatttgg gtttggccaa gccaagctcc gagtcgggat ctttagccga
      421 agctatgttg gccatccaaa ggtatccctg caggttgtcg gggtattttg tcaccattgc
      481 gttgaacaca gagtcagcct tgtcgaattc ctttgcctgg taaaaggcct ttccaacctg
      541 gatgtagtca ttttcagaat ttctacctag atcaataagg tgcatccagg tctgagcagc
      601 atcagcatat cttttggcgg catactgcac tgtacccttt tcataggtaa tgttcaagtc
      661 ctcctcaccg tcgaataggg ctgctttgtc gtatgcctcc agcgccaggg ttatctcagc
      721 ttccgacctt tcaaccaggg cttttaccga gtcaagctta gccttcaaag catcaatttc
      781 aattttggcc ttatccttgg ctggtccggg cttagctgag gcatacttgg tttgaagttt
      841 ggttaagtcg gcctccaggt tgtcagcctc aatagttagc tttcctaagt tctggttctt
      901 gcgtgaaagt attcgggcgt agtaaaggta gtccttctta ataaggcgtt cggggtcgag
      961 agaggcaaaa agcttatcca tgtaattctt ggccagctcg tagtttccct cctcaaaagc
     1021 agagtatcct gcaattcggt taaggtaggc cctggagtca tcaatggcaa agatttcctc
     1081 cacgtttttg attacttcct tgtattcctt ggcgtagaag agggcgttaa catacctgat
     1141 ctttgcagga atgttaccct tggtaagctc gaggtatttt tcaaagtatt tttttgattc
     1201 gttgaatcgt ccagccatgg agtatagctg gcccagctcg cgataggccg gggcgtagtt
     1261 ctgatcgagg gcaatggctt gctcgtagaa gggaatagca gccattaggt tacggccctt
     1321 cacgtaaatg cttccaattt tcatgttggc agttggtgaa gtggggtccc agtcctgagc
     1381 caggttgtag ttctttattg ccttggagcc gtcgtttacc aggatgtaca aatcgccggc
     1441 aatgatgtaa acctcgctgt ttcgtgaatc aatttgtagc gcttgtcgaa tgtagggcat
     1501 agccttactg gtatcaacct gtccaaaacg tatgtaggat tcgccaatct tggcaagagt
     1561 aaaggcataa tccttaggat ttggaatttt gctgaccttc ttgtacggcg gtaaaaggct
     1621 tttggccttt agcctaaact cctcggcctt ggcatcatcc ctgttgtaaa atgaaaccct
     1681 ggctaaacca acatagttta gcggttcatt ggggcttgcc gtgatgccat tgttaaatat
     1741 gtcggcagct ttcttggtag ccacgttcag cgagttggag attgtatcgg caaagtagtc
     1801 caaaaggaca ttttccccat aaaagaaata taccttgctg tttcccggct cgtttttaat
     1861 gagctgctga aacatggctt ccgctttatc atactgctca ttcttagtta atgtgatggc
     1921 ctcctgaaga gtctgagcct gtaaaccaac gccacaggtc aagcttaata aggcaacagg
     1981 aagcaatgct cggttaatac gtctcatgtt cattgctgtt aattttagtt actcgttttt
     2041 tacttcaaca attctaatcg gcatggtagc cgggaccaag cccgacttta gtatgattct
     2101 ttgacccttt tcacctgcaa caaaacttat gaatccggat cctaggccgt agaatgactc
     2161 ccttgagttt atgttaacct ccctgataaa ggggtaggtt ttctggtaaa ttgacccctg
     2221 aacgggaagg tagtaggaat ccttatcgaa atactgctgc gaaatggctg ctagctttac
     2281 cttgctgcca aggtcaaaac ttgtggaatc atcggtatcg cttatccagt ttacgctaac
     2341 tattccgaga gcaccaggac tcttagccac gtagtttaca acttctgcat tcgagtttac
     2401 agcgtagaag ttggggggta gctgctgtgg aaggttgaac ttttccctga agtaccggat
     2461 atttcctgag tggatgttgt cgaatataat gctaatctta cctagtttgg aacgggtatt
     2521 cagctggttc cagtcggtga tttttccggt aaaaatatcc ttaacattat cgtaggttaa
     2581 aagggagtcg ttatttccct tgttcagcac aattgccaac gcatcatagg caaccagcgt
     2641 agtacgaaca acagtttgag catcaagcag ggttttcctt tgctcctcgg ttggagtcca
     2701 tgacgaaacg attacctgca ccgaatcgtt gagaaaatcg gcaataagct ccctctcggg
     2761 tttgtaaacg gtttggatat gggcaaggtt atagagcgag gtgaatgtgc taacctcagc
     2821 atctacaatg ggcttgaacg attcatctgc ttcaatcttt atgctaccag aggtcggagt
     2881 ttcggcaggg acctttgctc ccttgttttt acatgcgggt agcacagcca caatggctac
     2941 agctaaaatc gccagctgta cgacttgttt gaatgttgaa atttttccca tagtattaaa
     3001 tttttccggc caacaactaa tattcttcgc tttgccctat tcaatacttt aacaacgcac
     3061 aatattagta attatcttga aaacttatag tatttaggta tcttcaaatt tcagtccata
     3121 atagttaata ccctgctagc tatcaacttg ttaccattat catttaatac tttttaacat
     3181 tagagcgggc aaatatacac caaccccgca tgtaaaaaaa cacctgctta acatttgcat
     3241 gcgaaacagg ctcaacaagc taacagaaag gggggaagaa tatattgaga gcttttcatg
     3301 catgtgctgc tactcgtcat catcatcctt gctaaaaaaa accttcttta tcttttcata
     3361 cgaatacatt atccggtaaa ttccgtatat aattaacgtt atgccgaata tcagccttag
     3421 ggctttatcc aaatggtcga gaagaggatg aaagatgatg aacaccccta acccgatata
     3481 aaagaatacc atgaataccc cgaaaatggc gaagacctta tcgtacattt taccagtgct
     3541 catttttcaa cgtttaaaat tcaacaagtg tgctgcatgt aagcagaacc taccaactta
     3601 agccttaact tctaatgccc agctttaagt gaacgctaca aaaataaaac tttaccttta
     3661 aacttatcat gacatatttc atatttttgt atggttgaaa cattcaaaag gataacaacc
     3721 tgctaatgtt gacctttaaa caaatgcagt atagctacat ttcagaacgg ctggttgttt
     3781 ttcaacctgc aatttcaagg cagcaaagtt ggtatgtttt aaagcatttt tttgaatttg
     3841 tatatttcgt ctcatggcaa tcttgctgtt tttggtagaa tatggaatct taaaaaaatc
     3901 actttttgat gaagtactta cacataaccc agctaattgt atttacgtgt ttatgcacta
     3961 cccttcacgc tcagcatccc gaacgctact tccggtttgt tgaacccagt aaagaaacta
     4021 tcaataccaa aattacccgc attatttcca ttgacagggt taaggccgat acggtatggg
     4081 cttacgccaa cgatgaggag tttgcccgat tctcggagct gggttacaag gtggagctgc
     4141 taacccctcc ctcgctgctc cccggaaaag t
//
LOCUS       DAOPHX010000179         4155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_29122_length_4155_cov_5.013659, whole genome shotgun sequence.
ACCESSION   DAOPHX010000179 DAOPHX010000000
VERSION     DAOPHX010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29122_length_4155_cov_5.013659"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PK502_10140"
     CDS             <1..858
                     /locus_tag="PK502_10140"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01217.1"
                     /translation="FIYNRSNDKLTTNHLGVGVFSKSPYLSYSGALRFYVNGYRSNEK
                     LLFGGITISPWKSTEMPFIKGSITFQDRQPDLFVRNYFSNHYAWSNNFEREKWLTVSG
                     VLGADRYKFEIGYNVANVVNYIYFDTTGRPAQASNVAVTSAYVQKVFKVRGLYMVGKV
                     LWQANTNSEVLSLPTLSGFGSIYYEYPLVKNVLTGQLGISVFYRTKFYADSYNPSTGQ
                     FINQREKKIGNYPFADVFANFKWKRANIFLKFEHVNQGLFSNEYFTALHYPANRRIFK
                     FGVSWMFYD"
     gene            997..2352
                     /locus_tag="PK502_10145"
     CDS             997..2352
                     /locus_tag="PK502_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPH01218.1"
                     /translation="MFSCISQDSVTRNQGHEVRDLADVLAEGKLVAVTDYNSASYYIY
                     KGEPMGYQFDMLRDFASFLGVKLELIAENDIQSSIEMLNSREVDLIANNLTITPERME
                     ELSFTLPHGETRQVIVQRKQPKPGEKGQTYIRSSQELAGKIVYVQRGSVSAKILKNIT
                     EKNGRSTVLVELPGYDAFQLIDMVAQGKIDYVVCDEVEAKVKVAVYSNLSCEIAVSNL
                     QPTAWAVRQSSPELLKRINLWLENYLSTSKYARIRSKYYENPLQARRMSNDYFYVYQG
                     KVSQWDEYFKHNSEKIGWDWRLLASMVYQESHFDHNAVSPRGAVGLMQFMPTTADYFG
                     IALNEMPIRQIEAGVGYLKWIEDRLSSFNIPKKEEVKFVLAAYNAGIGHVIDARNLAR
                     KYGRNPNVWDDNVEYFISNKAQFSGDPVVKYGSLKGKETTRYVEEILERYQHYQNIVE
                     K"
     gene            2449..3978
                     /gene="gltX"
                     /locus_tag="PK502_10150"
     CDS             2449..3978
                     /gene="gltX"
                     /locus_tag="PK502_10150"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPH01219.1"
                     /translation="MENRRVRVRFAPSPTGPLHIGGVRTALFNYFFARQHNGDFILRI
                     EDTDSQRFVPGAEEYINEALGWCGIKIDEGVKEGGLHAPYRQSERRQIYRQYADRLVA
                     SGNAYYAFDKPETLQKLRNEAENRGEAFTYNYANRNSMENSHTLGEDEVRNRIEGGDQ
                     WVIRYKMPADEEVVMHDIIRGEIRVNTSTLDDKVLFKSSDMLPTYHLANVTDDYLMEI
                     SHVIRGEEWLPSLPLHVLLYRALGWEDKMPQFAHLPLLLKPTGNGKLSKRDGDKMGFP
                     VFPLLWKAENGEVYRGYREDGYFPEAFVNLLALLGWNPGTERELFNMEELIQIFSLER
                     VNKSGARFDPEKAKWFNHQYLIRKGNDEVANLFMPILEQYGVQVQMDKLVRIVGLVKE
                     RVNFIHELWGQTSFFFERPLNYNSQTVAKFWNNDSSVMMNILVEKLSGISTWTALNIE
                     QSVKELIGEKGYGMGKVMNTIRLALVGESKGPGVADICEILGKEETLQRIRQAVLILG
                     K"
BASE COUNT         1170 a          836 c          999 g         1150 t
ORIGIN      
        1 tttatttata acaggagtaa tgataagctt actaccaacc atttgggcgt tggagtgttt
       61 agtaaatctc cctacttgtc gtattcaggt gcgttaaggt tctacgttaa tggttatcgt
      121 tcaaacgaaa agttactatt cgggggtatc actatttcac cgtggaaatc aaccgaaatg
      181 ccttttatca agggaagtat tacttttcag gacaggcaac ccgatttgtt tgttaggaat
      241 tacttttcaa atcattatgc ctggagcaat aattttgaaa gggagaagtg gttgacagtt
      301 tcaggagtgc tcggtgctga ccggtacaag tttgaaattg gatataatgt tgcgaatgtt
      361 gttaactata tctactttga cactactggc agacctgctc aggcttccaa tgttgccgtt
      421 acatctgctt atgtgcaaaa ggtattcaaa gtaagagggt tgtacatggt tgggaaggtg
      481 ctttggcagg cgaataccaa ttcagaagtg cttagcctac caactctttc aggttttgga
      541 tcgatttact acgagtaccc actggtgaaa aatgttttga ctggccagct tggtattagt
      601 gtattttata gaaccaaatt ttatgccgat agctacaacc catcgaccgg ccagttcatc
      661 aaccaaaggg agaagaaaat tggcaactac ccctttgccg atgtgtttgc aaattttaaa
      721 tggaaacgag ccaacatctt tctgaagttc gaacatgtta accagggcct gtttagtaac
      781 gaatacttta cagcgcttca ctatcccgcc aaccgtcgaa ttttcaagtt tggagtttcg
      841 tggatgtttt acgactagtt atcaaaatcc atctctgatt atcagttgat taacctaatt
      901 ttgctttttt acaggtttaa ccctaaagga cgagctatgc ggtcatcttt tgtttacaag
      961 tgcataaagt ggagtttatt tttgttttcc ctaataatgt tttcctgcat aagccaggat
     1021 agtgttacca ggaaccaagg ccatgaggtt agagatttgg ccgatgtgct ggctgaaggt
     1081 aagttggtgg cagtaacaga ttataactca gcaagttatt atatttacaa gggagagccc
     1141 atgggctacc agttcgacat gctacgcgat tttgcctcat ttttgggagt aaagctcgag
     1201 ctcattgccg agaacgatat tcaatcctct attgaaatgc taaatagccg ggaagttgac
     1261 ctaattgcca acaaccttac cataaccccc gaacgcatgg aggagttaag ctttacgtta
     1321 ccccatggcg aaacccgtca ggttatagtt caaaggaagc agccaaaacc cggagaaaaa
     1381 ggacaaacct atattcgcag ctctcaagag ttggcaggaa aaattgttta cgttcaacgg
     1441 ggatcggtat cagctaaaat attaaagaat attaccgaaa aaaatgggag gagtactgtc
     1501 ctggttgagc ttcctggcta tgatgctttt cagctaatag atatggttgc gcagggtaag
     1561 attgactatg tggtctgcga cgaagtcgaa gccaaagtca aggttgccgt ttacagtaac
     1621 cttagctgcg aaattgcagt tagtaatctt caaccgacag catgggcagt tcgtcaatca
     1681 tcacccgagc tcctcaagcg gattaatttg tggctggaga actacctgtc aacctcgaag
     1741 tatgcccgga tacgttcaaa atattatgaa aatccccttc aggcacggcg catgagcaac
     1801 gactattttt atgtttacca ggggaaggtt tcgcaatggg atgaatactt taaacataat
     1861 agtgaaaaga taggttggga ttggcggtta ctagcttcca tggtttatca ggaatcgcac
     1921 tttgaccata atgccgtatc acccagaggc gcagtaggtc ttatgcaatt catgcccaca
     1981 actgccgatt attttggtat agcccttaat gaaatgccta taaggcagat agaggctggt
     2041 gttgggtacc ttaaatggat agaggacagg ctgagcagct tcaatatccc taaaaaagaa
     2101 gaggttaaat ttgtgctggc tgcttataac gccggaatag gtcatgttat agatgcccgt
     2161 aacttggcca gaaagtatgg ccgtaaccct aatgtttggg acgataatgt tgagtacttc
     2221 atatccaata aggcccaatt ttctggcgat cccgtagtca aatatggaag tcttaagggt
     2281 aaggaaacta cacggtatgt tgaagaaatc ctcgaaaggt accagcacta ccaaaacatt
     2341 gtggaaaagt aaatggtaga ttttttgtag ccagctgtac ttgttttttc gggttgacgt
     2401 ttatagccca cttattattc ataattttga cctcacaaaa aatttgaaat ggagaaccga
     2461 agagtacggg ttcgttttgc ccctagtcca accggaccgt tgcacattgg aggggttaga
     2521 acagccctgt tcaactactt ttttgcccgc cagcataacg gcgattttat ccttagaatt
     2581 gaggatactg attctcagcg gtttgtacca ggtgccgagg agtatatcaa cgaggcgctt
     2641 ggctggtgcg gaattaaaat tgacgaagga gttaaagagg gtgggcttca tgctccctac
     2701 cgccaaagcg aacgtaggca aatttacaga caatatgccg ataggctggt ggctagtgga
     2761 aatgcttact atgcgtttga taaacccgaa acacttcaga aactccgcaa tgaggcagaa
     2821 aacaggggag aagcttttac ctataattat gccaaccgca actccatgga aaactcgcat
     2881 actttaggcg aggatgaggt tcgcaatcga attgaaggcg gcgaccagtg ggttattagg
     2941 tataaaatgc ctgctgacga ggaggttgtc atgcacgaca tcattcgagg cgagattagg
     3001 gttaacacct ccacgcttga cgataaggtg ctgtttaaga gtagcgacat gcttccaacc
     3061 taccatctgg ccaatgtaac cgatgactac ctgatggaaa tttcgcatgt tatcagagga
     3121 gaggaatggc ttccatcgct acccctgcac gttttgttat accgcgccct tggatgggag
     3181 gacaaaatgc cacagtttgc ccatttgccc ctactgctta aacctaccgg aaatggaaag
     3241 ctaagcaagc gtgatggaga caaaatgggt tttcctgtgt ttcctctact ttggaaggcc
     3301 gaaaatggtg aggtgtatag aggctaccgt gaggatggct attttcctga agcatttgtc
     3361 aacctgcttg ccctgcttgg atggaatccg ggaacagaaa gggagctctt caatatggag
     3421 gagctaattc aaattttcag cctcgagcgg gtaaataagt ccggtgcccg attcgatccc
     3481 gagaaggcaa agtggttcaa ccaccagtac ttaatccgca agggcaacga tgaggttgcc
     3541 aaccttttta tgcctatttt ggagcagtat ggcgttcagg ttcaaatgga caaacttgtc
     3601 cgaattgttg ggctggtcaa ggagcgagtc aattttattc atgagctttg gggccaaacc
     3661 agcttctttt ttgaaaggcc actgaactac aatagccaaa cggttgccaa gttttggaac
     3721 aatgattctt ccgtgatgat gaatatcctg gtagaaaagc tatccggaat ttcaacatgg
     3781 acagcgctca acattgagca atcggtaaag gagctgattg gagaaaaggg ctatggtatg
     3841 ggtaaagtta tgaataccat tcggcttgcg cttgttggcg aatcaaaggg ccctggcgtt
     3901 gccgatattt gcgagattct tggcaaagag gaaaccctac agcgaattag gcaggccgtt
     3961 ttaattctgg gtaagtagag ctttaggggg ttaagccaaa gggtaaattc tctcattact
     4021 gtgtttactc cggtaaacgt tgcgccaact agtaggatgg cttgcccaaa acaactacct
     4081 gaataatttc cggcactgtt gagctctcaa gccgtttggc cgctttgtcg aaggtgctta
     4141 agccagcagc ctccc
//
LOCUS       DAOPHX010000180         4080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_29795_length_4080_cov_5.186087, whole genome shotgun sequence.
ACCESSION   DAOPHX010000180 DAOPHX010000000
VERSION     DAOPHX010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29795_length_4080_cov_5.186087"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            460..2520
                     /locus_tag="PK502_10155"
     CDS             460..2520
                     /locus_tag="PK502_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPH01220.1"
                     /translation="MITRELINPQSIVVVGGSADVQKPGGKVLKNLIDHNFKGKLYVV
                     NPKADEVQGVRAYPDAEDLPPVELAILAIAAKFCPQAVETLAAKKGTKAFIILSAGFH
                     EESEEGARLEQQIVETINKAGGCLIGPNCIGVMNTNYAGVFTTPIPRFNTHGADFITG
                     SGATAVFIMESGISNGLQFNSVYSVGNSAQLGVEDILEYLDLTFDPNTSSRVKMLYIE
                     SIGQPQKLLKHASSLVRKGCRIAAVKAGSSSAGSRAASSHTGALASSDVAVDALFRKA
                     GIVRCYGREELTTVASIFMHPRLTGKNIAVITHAGGPAVMLTDALSNNGLEVPPISGS
                     KANELLSKLFPGSSVANPIDFLATGTAEQLGYIIDACENDFSHIDAMAVIFGSPGLFP
                     VYDVYDLLDRKMSECHKPIFPILTSVINVRDEINQFIAKGRVNFPDEVVFGNALTKVY
                     NTPDPAPEDPKLPIIDTSRIRRVIDGCSNGYLQPNQVQELLDAAGIPRTGEAIVKTAT
                     DATSAAMKMGFPVVMKVVGPIHKTDVGGVVLDVRDEQMVTREFNRMMGIKDTTAVLIQ
                     PMLKGLELFVGAKREGLFGHVVLCGLGGIFIEVLKDVKAGIAPIEANEAHSMIKHLQG
                     YGMIKGARGQEPVNENLFADIISRVSVLVGVAPEIFEMDINPLLGTKDKVVAVDARIR
                     IEKK"
     gene            2532..2684
                     /locus_tag="PK502_10160"
     CDS             2532..2684
                     /locus_tag="PK502_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01221.1"
                     /translation="MSIKGVFKKLSKNEKILLVIIILLLIMVATQWEKIWNEAIRAFG
                     RYFGSK"
     gene            complement(2861..3310)
                     /locus_tag="PK502_10165"
     CDS             complement(2861..3310)
                     /locus_tag="PK502_10165"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPH01222.1"
                     /translation="MALDNEAQLAGLLAHQLAHIDCRHITKMLLRHYDLSTFESIAAG
                     LEQAALDSIIHGLVNGSSTLKYSCSNEHEADSKAMEYLFDTGYDPYELINMYRKLLTC
                     SGVAQFFAIHPWDDGRIENLEAAWKNLGARHGEKYTERYLDFKKSLP"
     gene            complement(3310..3645)
                     /locus_tag="PK502_10170"
     CDS             complement(3310..3645)
                     /locus_tag="PK502_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01223.1"
                     /translation="MKKHLHIPLLLLMLASCSEDKSVFPTLTQDVCIGWRIDSAITAN
                     PAQFNVLERSKYAAIYSYTEEILQQLLESDGVRNAEAFSWQIRVINDNRLSVHLLLRE
                     VTSTSTRAY"
     gene            complement(3703..>4080)
                     /locus_tag="PK502_10175"
     CDS             complement(3703..>4080)
                     /locus_tag="PK502_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphofructokinase"
                     /protein_id="HPH01224.1"
                     /translation="YGHAKLGGIGDVVSRMIKEISPKFNNGKSVNVITQNLGYLVRGG
                     EPDAIDSIVPMAFGNLALDLIIKGIHGRLVVLKNGRYDDVPIDVVISQKKVVNVSKYY
                     NTERLRPFYKSFEMQPLFIMTSD"
BASE COUNT         1168 a          890 c          953 g         1069 t
ORIGIN      
        1 atggctttcc ccttttgcta catgcctaat tgaagacatg gcggggtgag agatgaagct
       61 gggcgtaaaa acccatttca ggctgcaggg aaatggttta tgctgaaacc cgattttttt
      121 ttggcacaat cctttcatgt ttaaaagcat aattttatct tctgtaacaa aataaaagtc
      181 cgggttttca tgaacaaccc tgtgaattgc catgcaatca ggaagaaatt aaaattaaag
      241 gtaatatgag taaacttggc agctagtaaa gttttgacct taaaaagttg aacaatagtg
      301 aaattgactg gctgaaatcg caaaccaata aaatggcata gttcgtagtc catttttata
      361 ggttctcact ttcaaattga agcttgctac attgctttat tagcattacg tttgcaaccg
      421 gaaaaatatc ctgaaaaaac aggtttaaaa tactttttta tgataaccag agagctgatt
      481 aacccccaga gtatagttgt agtgggtggt tctgccgacg ttcagaaacc cggtggtaag
      541 gttttgaaaa accttattga ccataatttt aagggtaagc tatacgtagt aaaccccaag
      601 gctgatgagg ttcagggtgt cagggcatat cctgacgccg aagatttacc acctgttgaa
      661 cttgccattc tggccatcgc agcaaagttc tgcccacagg cagtagaaac gctggctgca
      721 aaaaagggga caaaagcatt tatcatccta tcagccgggt ttcatgagga aagcgaggag
      781 ggtgccaggc tggagcagca aatagttgaa acgataaaca aagctggtgg atgtttgatt
      841 ggacccaact gcattggggt aatgaatacc aactatgccg gtgtttttac tactcctatt
      901 ccacgcttta atacgcatgg cgcagatttc attaccggtt cgggagcaac ggccgtattt
      961 atcatggaat caggaatttc caatggactt cagttcaaca gcgtttactc ggttggcaac
     1021 agcgcccagc ttggagttga ggacattctg gagtacctgg acctaacctt cgatccaaac
     1081 accagctcca gagtaaaaat gctttacatt gaaagcatag gccaacccca aaaacttctt
     1141 aaacatgcct catcgcttgt gagaaagggt tgccggattg cagctgttaa ggcaggaagc
     1201 tcatcagcag gaagccgggc tgcttcaagc cacaccggag cactcgccag cagcgatgtg
     1261 gctgttgatg cactatttag aaaagcgggt attgttaggt gctacggacg ggaagagctc
     1321 acaaccgttg cctcaatatt catgcatcca aggttaaccg gaaagaatat tgcagtaata
     1381 acccatgcgg gtggcccggc ggtaatgctt accgatgccc tatcgaacaa cgggcttgag
     1441 gttcccccta ttagcggttc aaaagctaac gaactactct ccaagctttt tcccggttca
     1501 tcggtagcca acccaattga tttcctggca accggcacgg ccgagcagct gggatacata
     1561 attgatgcat gtgaaaacga ctttagccat attgacgcaa tggcagtaat ctttggtagt
     1621 cccggattat tccctgttta cgatgtttac gacctcctgg atagaaaaat gtctgaatgc
     1681 cacaagccca ttttccccat ccttacctcg gtaatcaacg tcagggatga gattaaccag
     1741 ttcatagcaa agggcagagt aaacttcccc gatgaagtag tgtttggaaa tgcccttaca
     1801 aaggtttaca acacaccgga tcctgctcct gaagacccca agttgccgat aatagacacc
     1861 agcaggattc gtagggtgat tgatggctgt tccaatggtt acctacaacc caaccaggtt
     1921 caggagctac tcgatgctgc cgggattccc cgaactggcg aagccattgt aaaaacagcc
     1981 acagatgcaa cttctgcagc aatgaaaatg ggatttcctg tggtaatgaa ggttgtagga
     2041 cctattcata aaacagatgt gggaggagtt gtgctggatg ttcgcgatga gcaaatggtt
     2101 accagggagt ttaacaggat gatgggaatt aaggatacca ctgctgtgct cattcaaccc
     2161 atgctcaagg gtttagaact gtttgtagga gccaagcgcg agggtttgtt tggccacgtc
     2221 gttctgtgcg ggcttggcgg tatttttatt gaagtactaa aggacgtgaa ggccggcatt
     2281 gctccaattg aagccaacga ggctcattcc atgattaaac acctgcaggg ctatggaatg
     2341 attaaagggg ctaggggcca agagcctgta aacgaaaact tgtttgccga catcatcagc
     2401 cgggtttcgg tgttggtggg tgtggcgccc gaaatatttg aaatggacat caaccccttg
     2461 ctcggaacaa aggacaaagt ggtagctgtt gatgcgcgaa tcagaataga aaagaaatag
     2521 cctagctggt aatgagcata aagggtgttt ttaaaaaact ttccaaaaac gaaaaaatac
     2581 tactcgtcat cattatcctt ttgctgatta tggtagctac ccaatgggaa aaaatctgga
     2641 acgaggctat cagagcgttt ggcaggtact ttggcagcaa gtaaaccgta ttcttttaag
     2701 gaatggaaaa gcctagttgt gggggcattt tcacatttac tttcatcggg aaaatcgtga
     2761 gtggtgcgat tacccctttt ttcaatacaa gctgggtttt aaagtctata ccaaatgagt
     2821 ttgatttgcc cctttagaag gggctaaaat acaggaatca tcaaggtaaa gattttttaa
     2881 aatccaggta gcgttctgta tatttttcgc catgcctggc acccagattt ttccacgcag
     2941 cttccaagtt ttctatcctc ccatcatccc atgggtgaat agcaaagaat tgagcaaccc
     3001 cagagcatgt aagtagcttc ctgtacatgt tgataagctc atagggatca tagcctgtat
     3061 cgaacaggta ttccatggcc ttactatcgg cttcatgttc gttgctgcag ctgtatttta
     3121 gggtagagct cccgttgacc aacccatgga ttatactatc gagtgcagct tgttcgagac
     3181 cagcagcaat actttcaaac gttgataaat cataatgcct taaaagcatc ttggttatat
     3241 gccggcaatc aatgtgggcc agctggtgag ccagtagccc tgccagctga gcttcgttat
     3301 caagagccat taatatgccc gtgtagaggt agatgtaacc tcccggagca acaaatgcac
     3361 tgataaccga ttatcattta tcaccctgat ttgccagcta aaagcttcag cattgcgtac
     3421 gccgtccgat tccaaaagct gctgcaggat ttcttcggta tagctgtaaa tggcagcata
     3481 cttactacgc tccagcacgt tgaattgcgc tggattggcg gtaatggcag agtcaattct
     3541 ccaaccaata caaacatcct gcgttagggt aggaaaaacg gacttatcct ccgagcagga
     3601 agcaagcatc agcagcaata atggaatatg caaatgcttt ttcatcagga cataatgaag
     3661 ccaaacctac cgtagattaa aatatgcagc tcaaagtggc ggctagtcgc tggtcatgat
     3721 gaagagtggc tgcatttcaa agctcttata gaaaggcctt agcctttcgg tattgtagta
     3781 ctttgaaacg ttcacaacct ttttctggct aatcacaacg tcgataggca catcgtcgta
     3841 gcggccgttt ttaagcacaa ccaggcgacc atgaatcccc ttgatgatga gatcgagggc
     3901 aaggtttcca aaggccatgg gaacaataga atcaatagca tcgggctctc ccccacgaac
     3961 aaggtatccg agattctggg tgatcacgtt taccgacttc ccgttattaa atttaggaga
     4021 tatctcctta atcatcctcg aaacgacatc tccaattccc ccaagcttgg catggccgta
//
LOCUS       DAOPHX010000181         4040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_30213_length_4040_cov_5.083563, whole genome shotgun sequence.
ACCESSION   DAOPHX010000181 DAOPHX010000000
VERSION     DAOPHX010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30213_length_4040_cov_5.083563"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="PK502_10180"
     CDS             <1..1378
                     /locus_tag="PK502_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01225.1"
                     /translation="GGDFPSLSNNPARIGVYKSGELTVTPSFKNQKVSSTYNGIKATD
                     TRNRLYFDNVGVVMSLKPNGSDDKGLVNLNMGVGYTRTNDFFSNSIATGDNPNNSIMD
                     FFAVKAKGYYYNDLLVDDNYNPFSESSAPWDVIMAWNTYLLYDTIAGTNGTEYDPALL
                     IGDGVVQKNAVSTKGGSGEFDISFAANISNKLYLGLTVGIADYSFTYNATYSEDAFQS
                     NPARSNGDRFYYSDYMQYFETSGTGYNIKLGAIYTPIPSIRIGAAIHTPTFFSFSDSY
                     SYTMKSNFDIAGVESNFTSNSPLGKYDYDFETPFKLVGSFAYIIKEYGLVSLDVEHLD
                     YSAMRFRDGGNGDNFTDLNQSIDGHFRNVTNIRVGGEARINDFSVRAGYAYYPSPYKS
                     GFLNDNSNTNQLSFGLGYRIGNFTIDAAYLQTLQNAKYKFYDLTDLNVVSTKTRDGKF
                     LITLGFRF"
     gene            complement(1433..2068)
                     /locus_tag="PK502_10185"
     CDS             complement(1433..2068)
                     /locus_tag="PK502_10185"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HPH01226.1"
                     /translation="MDRKVVLSTAYLPPIQYFAKLLAYDVVYIESAENYAKQSYRNRC
                     TILSANGPLNLSVPAVKSLGNHTPITKVGIDNSSEWQKNHWRAVESAYRSSPFFDFGA
                     DLLFPFYTQRFENLFELNMELLKVLLGFIGVVVDVRFTTTYLTSYPEEYHDCRYFIHP
                     KRSFKADDHMVWVEYYQVFSDRFGFVPNLSVIDLIFNEGLGATSILKSMNK"
     gene            complement(2183..3508)
                     /gene="lepB"
                     /locus_tag="PK502_10190"
     CDS             complement(2183..3508)
                     /gene="lepB"
                     /locus_tag="PK502_10190"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPH01227.1"
                     /translation="MRTLFKNRYFNLGLSSLIYILMVIWIGNYWLLLGLAVIFDFYIT
                     RKVNWTFWKKRGQKRTVLIEWVDAIVFAVIAALLIRMFLIEAYTIPTSSMEKTLMVGD
                     YLFVSKVSYGPKMPNTPISFPFVHNTLPFSKKAKSYVEWPRWPYKRLAGLGSIKRNDV
                     VVFNFPEGDTVVLQDPNSSYYTLARFYGRDYLWQNFDIVYRPVDKRDNYIKRCVAIPG
                     DTVRVVHGQLYVNGKEQVKIGERQYNYRIITNGGTINPKRFEELGIAKADQNYNPAES
                     EYIIPLTEGMLDRIKDYGNIVKIERMENTDSTRMWQMIFPHNPSYPWTEDNFGPLWIP
                     SKGATVGLNLDNLPLYERIIDVYENNDLAVRNGAIYINGKPSNSYTFKMDYYFMMGDN
                     RHNSADSRFWGFVPEDHVVGKGSFIWLSLDKDRHFPFNIRWKRIFSGIH"
     gene            complement(3688..>4040)
                     /locus_tag="PK502_10195"
     CDS             complement(3688..>4040)
                     /locus_tag="PK502_10195"
                     /inference="COORDINATES: protein motif:HMM:NF017024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HPH01228.1"
                     /translation="IGGYSVGIKEIHHLQKLDAPSGTAITIAGKITEEIPSITGWTLL
                     PKREDGKIPIEAIREGTVPGTHTISFSSDQDSILLTHEAKSRRGFALGAVMAAEFLHS
                     KVGFFTMDDLLAFD"
BASE COUNT         1165 a          919 c          858 g         1098 t
ORIGIN      
        1 ggggggcgat ttcccctccc taagcaataa tccggcaaga attggtgttt acaagtcagg
       61 agagctaacc gttacccctt catttaaaaa ccaaaaggtt tcttcaacct ataacggcat
      121 taaagctacg gacacaagga atcgcctgta cttcgataac gtgggtgttg taatgtcgct
      181 taaaccgaac ggaagcgacg ataagggctt ggttaaccta aacatgggag ttgggtacac
      241 ccgcacaaac gactttttct ccaactccat tgccaccggc gataatccca acaactccat
      301 tatggacttc tttgctgtta aggctaaggg gtactactat aacgatttgc tggttgatga
      361 taattacaat ccatttagcg aaagcagtgc tccctgggat gtgattatgg catggaacac
      421 atacttgcta tacgacacga tagcgggtac gaatggtaca gagtacgacc cagcattact
      481 tattggtgac ggagtagttc agaaaaacgc tgtatccact aagggcggtt ccggtgagtt
      541 cgacatttca tttgctgcaa acatttcaaa taagctctac cttggattaa cagtaggtat
      601 tgccgattac agcttcacct acaatgcgac ctacagtgag gatgcctttc aatccaaccc
      661 tgcacgatcc aacggcgatc gcttctacta ctccgactac atgcagtact ttgaaactag
      721 cggaacaggc tacaatatta aactcggggc tatatacacc cctataccaa gtataagaat
      781 tggtgcagcc atccacactc ccactttctt ctcgttcagc gacagctact catataccat
      841 gaaatccaac ttcgacattg ccggagttga gagtaacttt accagcaatt cgcctctggg
      901 taaatatgac tacgactttg aaaccccatt caaactcgtt ggaagctttg cctatattat
      961 aaaagaatac gggcttgtaa gcctggatgt ggaacattta gactactcag ccatgagatt
     1021 tcgggatggg gggaatggcg ataactttac tgatttaaac cagagcatcg acggtcattt
     1081 cagaaatgtt accaacatac gtgtaggcgg cgaggcacga attaacgatt tttccgtaag
     1141 ggccggttat gcttactacc catcgcccta caagtcgggt tttttaaacg ataattccaa
     1201 caccaaccag ctttcattcg gccttggtta ccggataggt aactttacca ttgatgcagc
     1261 atacctgcaa actctccaga atgccaagta taaattttac gatttgaccg atctcaatgt
     1321 ggtttccacc aaaaccaggg atggaaaatt cctgataacc ctgggattca ggttctaaga
     1381 ttacaaaata tcaccatcta acccgtacaa cctgcccaac acccgggcag gtttacttat
     1441 tcatggactt cagaatggaa gttgcgccta aaccctcgtt aaaaatcaaa tcaataacgc
     1501 taagattggg cacaaagcca aacctgtccg agaaaacctg gtagtactca acccaaacca
     1561 tgtggtcgtc tgctttgaat gaacgctttg ggtggataaa ataccggcaa tcatggtatt
     1621 cctccggata ggatgtcaaa taggttgtag taaaccgaac atcaacaacc acacctatga
     1681 acccgagtag aactttaagc agctccatgt tcagctcaaa aagattttcg aacctttggg
     1741 tgtagaaagg gaatagtaaa tcggcaccaa aatcaaaaaa gggtgaagag cggtaggccg
     1801 attcaactgc ccgccagtga tttttctgcc attcagacga gttgtcaatt ccaaccttag
     1861 tgattggtgt atggtttccc agcgacttaa cggcaggcac cgataagttt aaggggccgt
     1921 tagcgctcag aatggtgcaa cggttacggt acgactgttt ggcgtaattt tctgctgatt
     1981 caatatatac aacatcatat gccaggagct tggcaaagta ctgaatgggc ggcaggtagg
     2041 ctgttgataa aactacttta cggtccaact atttccttga gttattatta tgccctctac
     2101 atcaaccagt aaatattcca ccattagatg attaagcgag gtaaaatgat gctgatggca
     2161 aacacattat aacgattgaa ggttagtgaa tgccggaaaa aatccttttc caccggatgt
     2221 tgaaggggaa atgcctatcc ttatcgagtg ataaccagat gaaagaaccc ttacccacca
     2281 catgatcttc gggaacaaaa ccccagaatc ggctatcggc cgagttgtgg cggttgtcgc
     2341 ccatcataaa gtagtagtcc attttaaaag tgtaggaatt ggaaggcttt ccgttgatat
     2401 agatagctcc attacgaaca gccaggtcgt tattctcgta cacatcaatt atccgctcat
     2461 acagaggcag gttatccaag tttaggccga cagtagctcc tttggacggt atccacaacg
     2521 ggccgaagtt atcctctgtc catggatagc ttgggttatg aggaaaaatc atttgccaca
     2581 tcctggttga atcggtattc tccatcctct caatctttac aatattgccg tagtccttaa
     2641 ttcgatcgag cataccttca gttaatggga ttatatactc cgattcagca gggttgtagt
     2701 tttgatcggc ttttgcaatg cccaactctt caaaacgttt aggattaatc gttcccccat
     2761 tcgttataat acggtagttg tattgccttt cgccgatttt tacctgctct tttccattca
     2821 cgtaaagctg gccatggaca accctaacgg tatcgcccgg aatggcaacg caccgtttta
     2881 tgtagttgtc gcgtttgtca actggccggt aaacgatatc aaagttttgc caaaggtaat
     2941 cgcggccgta aaacctggcc agggtgtagt agctagagtt gggatcctgc agcacaacag
     3001 tatccccctc tggaaagttg aataccacca cgtcgttacg ctttatacta cccagcccgg
     3061 ccagccgctt atatggccat cgaggccact ccacatacga tttggccttc tttgaaaacg
     3121 gcagggtgtt gtgaacaaag gggaatgaaa ttggagtatt gggcatttta ggaccgtagc
     3181 taaccttgct gacaaaaaga taatcgccaa ccatcagggt cttttccatt gatgaggttg
     3241 gaatagtata ggcttcgatt aggaacatgc ggataagcag ggctgcaata accgcaaaaa
     3301 caatagcatc aacccattca ataagaactg tcctcttttg cccccgtttt ttccaaaatg
     3361 tccagttaac cttacgggtg atgtaaaaat caaaaattac ggctaatcct agtagtagcc
     3421 aatagttacc tatccagata accattaaaa tgtagatgag ggacgacaac cctaggttga
     3481 agtatctgtt tttgaacaag gttcgaattt tcgtggtcat attaccttaa gctgaagttt
     3541 acgctgcaaa actattattt aattgaactg gttgtagcct aaaggggtta caatatcctc
     3601 atattaacat tagttatttt aagcatgccg ctttaagcta taattatttg ttgatataaa
     3661 agaaaaagat tgcataccgt taccctctta atcaaaggca agcagatcgt ccatggtaaa
     3721 aaaacccact ttgctgtgca ggaattctgc agccatcacg gctcccagcg caaaaccacg
     3781 cctactttta gcttcgtggg ttaataaaat gctatcctga tcggagctaa aagaaatggt
     3841 gtgagttcct ggaaccgtcc cttcccttat ggcttcaata ggtattttgc catcctcacg
     3901 cttgggaagt aacgtccaac cggtaattga ggggatttct tcagtaattt taccggcaat
     3961 ggtaatagct gtgccgcttg gagcatccag tttttggagg tgatgtattt ccttaatgcc
     4021 caccgagtaa ccgccaattt
//
LOCUS       DAOPHX010000182         4036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_30254_length_4036_cov_6.628736, whole genome shotgun sequence.
ACCESSION   DAOPHX010000182 DAOPHX010000000
VERSION     DAOPHX010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30254_length_4036_cov_6.628736"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PK502_10200"
     CDS             <1..616
                     /locus_tag="PK502_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01229.1"
                     /translation="TGQGLYQKSWLVDVSAGTPLLEKGGTIPIQARISGGYEIQFTES
                     GILGLAIRGGYASTRGHRSLTDETGVVYDGGESLYSYTGNSLFVGVSPMIIFEQYDSN
                     FDRLFIELELGLASHNVDMKFYEPTKTSTSNWVHFNGYFALKAGFRLAWKKEEGYFRA
                     MSIWGSLHNQSPINAIEKSIPKNWPYEFKEDLKSQWQIGISVYI"
     gene            848..1648
                     /locus_tag="PK502_10205"
     CDS             848..1648
                     /locus_tag="PK502_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPH01230.1"
                     /translation="MEDYVELPICTIRYRLSGQGPAVILVHGYLENLEIWEDIVPDLE
                     KNYTVLRVDLPGHGKSACNHQTISMDLMADSISAVMTTLDIKKAFMVGHSMGGYTTLA
                     FAERYPNWLNGFCLLHSSANADSSEKRQSRMNDIETVKNGGKRDLVYLSIPRLFANQN
                     LEKMDDIVDKFIKIGLSTPDQGIIGALNGMAKRPDRNYVIENANIPVFFIFGKHDNLI
                     SMEVAEALMERHKKARTVILNNSGHMGFVEEKDQTTAAITSFLTQVYS"
     gene            complement(1639..3312)
                     /locus_tag="PK502_10210"
     CDS             complement(1639..3312)
                     /locus_tag="PK502_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPH01231.1"
                     /translation="MAYQNSKKDIYYPIVIALVLAAGILIGQRFNGKSQKPGTLVYNR
                     TDKLSSVISYIEEEYVDSISVSTLVENTIPAILKNLDPHSIYIPASELQSVSEPLEGG
                     FDGIGIQFNMSNDTAVVVNTIKGGPSERLGILAGDRIVKVNGKLIAGVKFPMDSIPIL
                     LKGPTGTKVKVEIKRKDVDELIDFEITRDKIPISSVDIAYMVTPEIGYIKITTFAKTT
                     YKEFLDAMAKLESQGMKKLILDLRSNGGGYMDAATKIANEFLKPNQLIVYTQGKSRTR
                     QDVYSTGTGKYQDIPLQVLIDEFSASASEIFAGAIQDNDRGQIVGRRSFGKGLVQEQI
                     FFSDGSALRLTIARYYTPSGRCIQKPYTPGDDNYFNDITSRYLHGEFEVADSIQFADS
                     LKYYTVGGRTVYGGGGIMPDIFVPLDTNGITPYLQQVSRRNLIYRFAFNFTDQHRKET
                     RGLKDFTSVDRFLNKFDLLSDFIKFAEQNGVPRNEKEISVSKKILDAQVKATIARNII
                     DNEGFYPYIRNIDETLLKAIGLMEQSVSQPTTSALHRSDNSRVLNQAQL"
     gene            complement(3394..3843)
                     /locus_tag="PK502_10215"
     CDS             complement(3394..3843)
                     /locus_tag="PK502_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HPH01232.1"
                     /translation="MNKTTSFNDPKQLQFDRRYLEMAKIWAKNSYCKRRQVGALIVKN
                     RMIISDGYNGTPSGFENICEDENGLTKPYVLHAEANAITKVAKSGNSSEGATLYVTSS
                     PCMECAKLIIQAGIKRVVFCDEYHNPDGLSLLSRAGIDVVKLDIEEL"
     gene            complement(3950..4033)
                     /locus_tag="PK502_10220"
     tRNA            complement(3950..4033)
                     /locus_tag="PK502_10220"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3997..3999),aa:Leu,seq:caa)
BASE COUNT         1095 a          811 c          869 g         1261 t
ORIGIN      
        1 aacaggccaa gggctgtacc aaaaatcttg gttggtagat gttagtgcag gaaccccgtt
       61 gcttgagaaa ggtggaacta tacctataca agcaagaata tcgggtggat atgaaataca
      121 gtttactgag tctggaattt tgggacttgc aattcgcgga ggatatgctt caacaagagg
      181 ccatagaagt cttacagatg aaactggtgt tgtttatgat ggaggcgaat cgctatatag
      241 ctacactgga aatagtttgt ttgttggtgt atcgcctatg ataatttttg agcaatacga
      301 tagcaatttc gataggttat ttattgaatt ggaattagga ttggctagcc ataatgttga
      361 tatgaaattt tatgaaccca ccaaaactag cacttcaaat tgggtccact ttaatggtta
      421 ttttgcgctt aaggcaggtt ttagacttgc ctggaagaag gaggagggct attttagggc
      481 aatgtcaatt tggggttctt tacacaatca aagtccaata aatgcaatag agaaatcgat
      541 tccaaagaat tggccttatg aatttaaaga ggatttgaaa tcgcaatggc aaattggtat
      601 ttctgtttat atttaacaaa taagttcgtt atattttaaa acgccgctaa tatttagatt
      661 agtggcgttt ttttgacatt gagtttaaaa attaaaccca ttagatcttg ttgcaatttg
      721 ttttttaatt ggtttgtatt agttttatct ggaatcaatt tgctatattg aacgctgtca
      781 gggtatagaa ccctggcagc gttaggataa ataagtttag actttgtata aaatcatttt
      841 ctgaattatg gaagactacg ttgagttacc aatttgcacc attcgctaca ggttaagcgg
      901 acaaggcccg gcggttattc ttgtccacgg ctatctggaa aaccttgaga tttgggaaga
      961 tattgttccc gatttggaga aaaactatac cgttctacgg gtcgatttgc ccggtcacgg
     1021 aaagtcggct tgtaatcatc aaaccattag tatggatctt atggccgata gtattagcgc
     1081 tgttatgacc accctcgaca ttaaaaaagc ctttatggtt ggacactcaa tgggtggtta
     1141 taccacgctt gcctttgcag aacgataccc taactggtta aacggctttt gcttgcttca
     1201 ctcatcggct aacgcagact cgtcggaaaa gcgtcagagc aggatgaatg acattgaaac
     1261 cgttaaaaat ggtggaaagc gcgaccttgt ttacctaagt attcctcgcc ttttcgccaa
     1321 ccagaattta gagaaaatgg acgacattgt tgataaattc atcaaaatag gactaagtac
     1381 acctgaccag ggaataatcg gggcgctgaa cggaatggca aaacgccccg ataggaatta
     1441 tgttattgag aatgcaaaca ttcctgtttt cttcatcttt ggaaaacacg acaacctgat
     1501 ttctatggag gttgcagagg ccttaatgga aaggcacaag aaagcccgaa ccgttattct
     1561 aaacaactcg ggccatatgg gttttgttga ggagaaggat caaacaaccg ctgccattac
     1621 atcgtttctt acccaggttt atagctgagc ctgattaagc acacgtgaat tatcactcct
     1681 atgaagagct gaggttgtag gttgactaac gctttgctcc attagcccaa ttgcttttag
     1741 caatgtttcg tctatattcc taatgtatgg ataaaatcct tcattatcaa taatatttcg
     1801 ggcgatagtt gcttttactt gtgcatccag aatctttttt gaaactgata tttccttttc
     1861 gtttctgggc acaccgtttt gctcggcaaa ctttataaag tcggagagca ggtcaaattt
     1921 attcaggaac ctatcaaccg aggtaaagtc ctttaaacct cgagtttctt tccgatgctg
     1981 atcggtgaag ttgaaggcaa accggtaaat gagatttctt cgggatactt gctgcaggta
     2041 gggggtaatt ccgttggtat cgagtgggac aaaaatatcg ggcataatgc ctcctcctcc
     2101 ataaacagtc cttccgccta ctgtgtagta ttttagcgag tcggcgaact gtatgctatc
     2161 tgcaacctca aattctccat gcaggtatcg gcttgtgata tcgttgaagt agttatcgtc
     2221 gccaggtgtg tagggttttt gaatgcatct acccgatggg gtgtagtagc gggcaatggt
     2281 gagccttagg gcagatccat cggaaaaaaa tatctgctcc tgaacgaggc ctttcccaaa
     2341 tgaacgcctt ccaactatct gtcccctgtc gttatcctgt atcgctccgg caaaaatttc
     2401 acttgccgaa gcggaaaatt cgtcaattaa aacctgtaaa ggaatatcct ggtactttcc
     2461 tgttccagta gagtaaacat cctgacgggt tcggctttta ccctgtgtgt aaacaatcag
     2521 ctggtttggc tttaggaact cgttggcaat tttggtggca gcatccatgt accctccacc
     2581 attgcttcgc aaatcaagta taagtttttt cattccctgg ctttcgagct tagccattgc
     2641 atctagaaat tccttatagg ttgttttggc aaaggttgta attttaatat accctatctc
     2701 gggtgttacc atataggcaa tgtccacgct acttattgga attttatccc ttgttatctc
     2761 gaaatcaata agctcgtcaa cgtcttttct tttgatttca accttaacct ttgtcccggt
     2821 agggcctttc aagagaatgg ggatgctgtc catcggaaat tttactcccg caatcagctt
     2881 tccgttgacc tttacaatcc tgtcaccggc caaaattccg agcctttccg aggggcctcc
     2941 ctttatggta ttaacaacta ctgctgtgtc attcgacatg ttgaattgaa ttccaattcc
     3001 gtcaaagccc ccttcgagag gttcgcttac agattgcagc tccgaagctg gtatgtaaat
     3061 ggagtgtggg tctaggtttt taagtatagc aggtatagtg ttttcgacca gggtagatac
     3121 agaaattgaa tctacatact cctcttctat gtagcttatc acgctgctca gcttatcagt
     3181 tcggttataa accagcgttc cgggcttttg gcttttccca ttaaatcgtt gaccaataag
     3241 tattcctgcc gccagtacta gggctatgac tataggatag taaatatcct ttttgctatt
     3301 ttggtatgcc attctattca agtaatttat tctgaagtta ttggtattaa aaccatcagg
     3361 ttgtactctt ttagaaatat aactttaaaa agcctaaagt tcctctatgt cgagttttac
     3421 cacatcaatt cctgctctac tcaaaagcga aagaccatcg ggattgtggt attcgtcgca
     3481 gaaaactacc cgttttatgc ccgcctgaat gattagcttg gcgcattcca tgcacggcga
     3541 cgaggttacg taaagggttg ctccttctga gctatttcct gatttagcca ccttggtaat
     3601 tgcatttgcc tcggcatgaa gaacataagg cttagtaaga ccattctcat cctcacagat
     3661 gttttcaaaa cctgagggtg ttccattgta cccatcagat attatcattc tatttttgac
     3721 aataagcgct cccacctgcc tacgcttaca gtaagagttt tttgcccaaa ttttagccat
     3781 ttccagataa cgtctgtcaa actgaagctg tttggggtca ttaaaactgg tcgtcttatt
     3841 catgtagggc taacgtttgc agcgttttgc tactatagat ttgaattata gctgtacagt
     3901 aaatggagaa aatgaagcaa cttttaagta aaactggtgg ttggataagt acccggggcc
     3961 gggatcgaac cggcatggta ttgctaccac tggtgtttga gaccagcgcg tctaccaatt
     4021 ccgccacccg ggcaat
//
LOCUS       DAOPHX010000183         3950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_31161_length_3950_cov_4.660334, whole genome shotgun sequence.
ACCESSION   DAOPHX010000183 DAOPHX010000000
VERSION     DAOPHX010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31161_length_3950_cov_4.660334"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PK502_10225"
     CDS             <1..645
                     /locus_tag="PK502_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01233.1"
                     /translation="VHMADNSATPEMLLDNIVLNGANGTDTFYVTKIYSWDELKQGYS
                     KLGNGIDDFPPDAASADVGDYFALKYVSVMDNGFESLNRSTTKILVANSYAGFYTSHI
                     TYFHPTLGGAYPDKPYINTYTQKQLITLSVTSCTTNFALWGSYGESMDITINPDNSVV
                     FNIVGFDYIVNEGDPYNPAYHSYYDPTNSTIHLYYYWEGVGGYRVIWETLSLNI"
     gene            763..>3950
                     /locus_tag="PK502_10230"
     CDS             763..>3950
                     /locus_tag="PK502_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01234.1"
                     /translation="MRKLFTGLTIAFILQLFSGISHAEGTKQLMPNSSDRLWLEFNVF
                     GTGSSSFAGYNCSAEKRLNIRLVAGEKVFFGFKMNTINYGGNVYTNPNNAYYRIRKPD
                     GSIAVAESNIPTSGAGYIANYSQAIAGPNGAKLNGVTITGGYSPKTFTADQTGDYYIE
                     LRYYNNYRFALEFFDITVTDSNNNIITNPGEPNKPAGRIWSKTWQLTTTSFTQYPVNN
                     YFYVFTSDDFVNKINFQYYPFSFTFQSNSYGLINPTNEPNYIKRAQSKDNDQTSAASE
                     YRIYLNDPDRNIWPTTRLAAPKTQVWANDTLFFDYQYDRTPQQLAFADHDIYLTKNKV
                     GCEYSSIAIFKINSNIDAFTAILIDLDGDGGYSTAGNDRVIYRNMRRGNNYILWNFKN
                     DAGAEVATGVYHASATFMGRGPTNFPVYDVEQCDGVITSSIRPFRKLNATIYWDDTYI
                     SRWGDETGHGLMDETQRKQLKVSTIIPRIWSWNANLENTNFNGNLNTMNTWFNAIDLG
                     YSDITIHVNQSTTQCINGLAPWVGDIYKQGLPNNTITFTQADFTTKFFDPIEDPLTRI
                     RIKSLPANGSLKLSGTAVTVNQEITLANLANLTFTPNTGWVGITQFDWEAKNSQFWSM
                     NADKVYITVNTPPTISSIADQNLCTNNPSPAIPFTVNDSETPAANLTVTAYSHDPTFV
                     PNSSITIGGSGTNRTISVTPVANKSGHAIIYVMVDDGLSQTIQEFAVYVGPDLEFSGD
                     TTICVGQDLYLVAQEVGASSYTWKYNGTVVASTQTLSKAWGSFSAGSWSLTVSKGGCV
                     STRNFQVTIFPLTTFTGDQNVCVGEEINLSATENNASYEWKKGTTIVSSTKQFVKPSA
                     TLSDAGTNYTLKVTKSGCSATSNPFAITVINAPNVGLTVTGSTVDPGRNGTITIGSAQ
                     SGVTYNIYKNETLITSGVGAGANLTITVPAANLSVGSNLFVVKADNGNCEVPLSSTAT
                     ITVREPGFTITVISANTTEAGGTASFTVVCKTQPLANVILQFTSTDLTEGTVVSSITF
                     TTANWNTPQTVTVTGVDDYVVDGNISYTIQVGP"
BASE COUNT         1138 a         1045 c          826 g          941 t
ORIGIN      
        1 gttcacatgg ccgataattc agccactcca gaaatgctgc ttgataatat tgttttaaac
       61 ggggcaaatg gcaccgacac cttttatgtt accaaaatct actcatggga tgagctgaag
      121 cagggctact ccaagctggg aaacggcata gatgattttc cccccgatgc tgccagcgcc
      181 gatgtgggcg attactttgc tttaaagtac gtatcggtga tggacaatgg attcgaatcc
      241 cttaaccgtt ccaccacaaa aattctagtt gcgaactcgt atgccggttt ctacacctcg
      301 cacatcactt actttcaccc aacacttggc ggtgcttacc cagacaaacc gtacattaat
      361 acctacaccc aaaagcagct aatcaccctt tccgtcacca gctgcaccac aaactttgcc
      421 ctctggggca gctacggaga gtcaatggat atcaccatta accccgacaa ctctgtggta
      481 ttcaatattg ttggttttga ctacatagtt aatgagggcg acccctacaa ccccgcttac
      541 cattcatact acgatcccac taacagcact attcacctct actactactg ggagggcgtt
      601 ggtggttacc gggtaatttg ggaaacctta tcccttaaca tttaagcata ccgaaaattt
      661 aacccagata gtggaaatgg gttgaacttg gtaaaaagtt aacaattatg acaaaccgcc
      721 aatggcaagc catacaatta aaactaattt aaggtacacg atatgagaaa gctatttacc
      781 ggattaacca ttgcttttat tctgcagttg tttagtggaa tttcacacgc cgagggaacc
      841 aagcagctca tgcccaactc ctccgacagg ctatggctgg agtttaacgt attcggcaca
      901 ggaagcagca gctttgccgg gtacaactgt tctgccgaaa aaaggctgaa catcaggctt
      961 gttgccggcg agaaggtttt ctttggcttt aagatgaaca ctataaacta tgggggaaat
     1021 gtatatacaa atcccaataa cgcctactac agaataagga agcccgatgg cagcatagct
     1081 gtggccgaat cgaatattcc aacatcagga gcaggataca ttgcaaatta ttcccaggcc
     1141 attgccggcc caaacggtgc caagctaaac ggtgttacca taaccggtgg ctacagccca
     1201 aaaaccttca ctgccgacca aaccggcgac tactacatcg agttaagata ttacaataac
     1261 tatcgttttg cgctggagtt cttcgacatc acggtaaccg attccaacaa caacattatc
     1321 accaacccag gcgagccaaa caagccggca ggtaggatat ggtcaaaaac atggcagcta
     1381 accaccacca gcttcaccca gtacccggtg aataactact tctacgtttt cacctccgat
     1441 gacttcgtga acaagatcaa cttccagtac tacccctttt cgtttacgtt tcagtctaac
     1501 tcctacggtt taataaaccc cactaacgag ccaaactaca tcaaaagggc acaaagcaag
     1561 gataacgatc aaacatcagc tgcatcggag tatagaatct acctcaacga tcccgacagg
     1621 aacatttggc ctaccacacg gttggccgct cctaaaactc aggtttgggc caacgatacc
     1681 ctcttcttcg actaccagta cgatcgcact ccccaacagc tggcttttgc tgatcacgac
     1741 atttacctca ccaagaataa ggtgggttgt gagtacagct ccattgctat tttcaagata
     1801 aactccaaca ttgatgcatt tactgctatt ttgatagacc tggacggcga tggaggctac
     1861 tccactgcag gtaacgatcg ggttatatac cgtaacatgc gaagagggaa taactatatc
     1921 ctctggaact tcaagaatga tgccggcgcc gaggtggcaa caggtgttta ccatgcttcg
     1981 gccaccttta tgggtagagg cccaacaaat tttcctgttt acgatgtgga gcagtgcgat
     2041 ggggtaatca cctcttccat ccgacccttc aggaagctta acgctaccat atactgggac
     2101 gatacctata tctcccgatg gggcgacgaa acaggacatg ggcttatgga cgaaactcag
     2161 cgtaagcagc ttaaggttag caccatcata ccccgtatat ggtcgtggaa tgcgaacctt
     2221 gaaaatacaa acttcaacgg caacttaaat accatgaata catggttcaa cgctatagac
     2281 ctgggatatt ccgatatcac cattcacgtg aaccagagca ctacccagtg catcaacggg
     2341 ttagcaccat gggttggaga tatttataag cagggactac ccaacaatac tataaccttt
     2401 acacaagctg attttacaac taaatttttt gatcctattg aggatcccct tacccgaatc
     2461 cgtataaaat cgctaccggc aaatggctcg cttaagctgt caggaacagc agttactgtg
     2521 aaccaggaaa tcactctggc caacctggcc aacctaacct ttaccccaaa taccggatgg
     2581 gttggcatta cccagttcga ctgggaggca aagaatagcc agttctggtc aatgaacgcc
     2641 gataaggttt acatcactgt aaacacacct cctaccatat catccattgc cgaccaaaac
     2701 ctgtgtacaa acaacccctc cccggccata ccttttacgg taaacgatag cgaaactccc
     2761 gcagccaacc ttaccgttac ggcatactct cacgacccaa cctttgttcc aaacagctcc
     2821 attaccattg gcggtagcgg aaccaaccgt accattagtg ttacccctgt agccaacaaa
     2881 tcgggccatg ccattattta cgttatggtt gatgacgggc tatcgcaaac cattcaggaa
     2941 tttgctgtat atgttggccc cgatttggaa tttagcgggg acaccaccat ttgtgttggt
     3001 caggatttgt acctggtggc tcaagaggta ggtgcctcat cctatacatg gaagtacaac
     3061 ggaactgtgg tggctagcac ccaaacccta tcaaaggcat ggggtagttt tagcgccggt
     3121 agctggtcgc taacggttag caagggtggt tgcgtttcaa cccggaactt ccaggtaacc
     3181 atattcccct taacaacttt cactggcgat caaaatgtat gcgttggtga ggaaatcaac
     3241 ctcagcgcca ccgagaacaa cgcctcctac gaatggaaaa aggggacaac cattgtttct
     3301 agcacaaaac agttcgtcaa acccagcgca acgcttagtg acgcaggtac caattatacc
     3361 cttaaggtta ccaaatcggg ctgctcagca acctccaacc cgtttgctat aacggtaata
     3421 aacgcaccca acgttggcct gacggttact ggcagcacgg ttgaccctgg gcgtaatgga
     3481 acaataacca ttggttctgc tcaatcgggt gttacctaca acatatacaa aaatgaaacc
     3541 ttaatcacat cgggagtggg agcaggagca aaccttacca taacagtccc ggctgccaac
     3601 ctctctgttg gttcaaacct ttttgtggtg aaggctgata acggcaactg cgaggtaccg
     3661 ctctccagca ccgcaaccat taccgtaagg gaacccggtt ttaccattac cgtaatatcg
     3721 gccaatacaa ccgaggccgg aggcaccgct tcctttaccg tggtatgcaa aacccaacct
     3781 ttagccaacg tgatccttca gtttaccagt accgatttga ccgagggaac agtagtctca
     3841 tccatcacct ttacaacagc aaactggaac acgcctcaaa cggttaccgt taccggtgtt
     3901 gacgattacg tggtggacgg aaacatcagc tacaccattc aggttggacc
//
LOCUS       DAOPHX010000184         3889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_31877_length_3889_cov_6.494262, whole genome shotgun sequence.
ACCESSION   DAOPHX010000184 DAOPHX010000000
VERSION     DAOPHX010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31877_length_3889_cov_6.494262"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PK502_10235"
     CDS             complement(<1..792)
                     /locus_tag="PK502_10235"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HPH01235.1"
                     /translation="MKGDGMEDNMNLKSNPQQQDLPEEEQLDVKKLLFRVLANWYWYA
                     ITLFISLFIAYLINRYSEQSFSVSSSVIVRDDDQTKSYVGAEQLIQSLRLVKNTKSVQ
                     NEIGILQSYSLARQTVEELEDFNVTYVAVGRRGIKEQKLYTGSPFKVVIDSGATNMQG
                     HPVRVNILNSREYRLEIDEGMGISRTMRFGEPFTNESFNFTVFLRDPLNYDPKVAGRK
                     YYFTINDPNTVALSYKSKVTVTLNDKKGSILTLNSTGYVAQQEADY"
     gene            complement(851..1624)
                     /locus_tag="PK502_10240"
     CDS             complement(851..1624)
                     /locus_tag="PK502_10240"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPH01236.1"
                     /translation="MRISRPAVLIIVFASLMQVSCNRKAQLLYLNNLDTGLLTSTQPA
                     PYQLRPGDVLSVQVITQDPEISRLFNIVSSGTSTSYTAYSNDASLYINGFSVNDSGMV
                     RLPVVGTMPVAGLTVRQAQDSIQLKYERYLKDALVVVKLLSFKVTVLGEVRRPGTYTN
                     YRDNLNIFEAIGLAGDFSDYGERTNVLVLRQTPEGVKTFRLNLQDKNLLTAEGFYLLP
                     NDTVIVEPRNAKVMALNTPNISIFLSIITTALLLLNYVK"
     gene            2040..>3889
                     /locus_tag="PK502_10245"
     CDS             2040..>3889
                     /locus_tag="PK502_10245"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPH01237.1"
                     /translation="MRRYILFALLGITVYTATGQPYNRLGNPLVTNFTPEDYDATEQT
                     WCITQDDRGVMYFGTYDKGILEYDGKTWRKIQVPDSKPVNSMIKGDDGVIYVGTSGDF
                     GFLEPTADGSQRFVSLSGSINDTIKQSLSHIYKTYYFKGNVLFCSCSYIFVYDKQGKL
                     TPVYLGYQPEYANFLTFVVNDKVYIGSYLKGLRMLNSDWTLSTAPGGSSFERINIYSI
                     VSLSPSELLLVTDSGFFVYNQKTGSSKKMEKQNNFIESMLSQSVIPYHAIKLHNGNIG
                     VGTVQSDWLSFFELNADSSSPSTIVNPQTGLQAAQITYLYQNGESPVWATLFDGGISR
                     IELHSPIRRFSTESGINELILDIIQFNGTLYVATINGVYYQTAAANGMSVFKPVEGIN
                     GVVWSLLKFTPPGGSPMLLAGSYVDGVFEIKGNRAISISKPLYRSFPNVQHQCLTLYQ
                     SVLHPNMLYVGGISRLTYIEWSKGEWVKTQNIALDEIRSEVWSIGEDARGNLWLGTNL
                     KGVYMIDPHEKVYNFNSSSDFSGFKLLRIYPDGDSLYVLTPKGIFYYSYSDSTFRKCG
                     LLGKNSLENGFSRLLKVGNEIVAACYNDSTGKYWVCTMAKNLSGEWVTND"
BASE COUNT          989 a          854 c          945 g         1101 t
ORIGIN      
        1 gtagtcggcc tcctgttgtg ccacgtagcc tgtgctgttt agcgtaagaa ttgaaccctt
       61 tttgtcgtta agggtaaccg taactttcga cttgtacgat agggctacgg tgttgggatc
      121 gttaatggta aagtagtact ttcgccctgc aaccttgggg tcataattca atggatcgcg
      181 caggaaaact gtaaagttga acgactcgtt ggtgaatggc tcgccaaacc tcatggtacg
      241 tgaaatgccc attccctcat caatctccaa acggtactcc ctgctgttca ggatgttaac
      301 cctaacagga tggccttgca tgttggttgc accggagtct atcactacct taaaggggga
      361 accggtgtaa agcttctgtt cctttattcc cctacggccc acggccacgt aggtaacgtt
      421 aaaatcctcc aactcctcca cggtttgccg ggccagggag tatgactgca gtatcccgat
      481 ttcattctga acgctcttgg tattcttcac cagccgcaag ctctgtatga gctgctcggc
      541 gccaacgtat gacttagtct ggtcgtcgtc gcgaacaatt acacttgatg agacgctgaa
      601 tgactgttcg gagtaccggt tgatgagata tgctataaaa agggagataa agagtgtgat
      661 ggcataccag taccagttgg ccagcacgcg gaacagcaac tttttaacat ccagctgctc
      721 ctcctcgggg aggtcctgct gttgtgggtt ggatttaaga ttcatgttgt cttccatacc
      781 gtctcccttc aatgtttttt taccctttag ttgcgattta ccaccaacat gaaccgttgt
      841 aatttcctct ttattttaca tagttaagca gcagcaaggc ggtggtgatg atggaaagga
      901 atatggaaat gttaggggta ttcagggcca tcaccttggc gtttcgtggc tccacaataa
      961 ccgtatcgtt tggcagcagg tagaacccct cggcggtaag cagattttta tcctgcaggt
     1021 tgagccggaa tgtttttacc ccctcgggtg tttggcggag cacaagcacg ttggttcgtt
     1081 caccgtaatc ggagaaatcg ccggcaagtc caattgcctc aaatatgttg aggttgtccc
     1141 tatagttggt gtaggttccg ggtcgtctta cttcgcccag caccgtaacc ttaaagctga
     1201 gcagcttcac caccaccaga gcatccttga ggtacctttc atatttaagc tgtatcgaat
     1261 cctgagcttg gcgaacggtc agccctgcta cgggcatcgt tccaacaacg ggcaggcgta
     1321 ccatgccgga gtcgtttacc gagaatccgt ttatgtaaag cgaggcatcg tttgagtaag
     1381 cagtgtagga ggtagaggta ccgctcgaaa caatgttgaa taggcggctg atttcgggat
     1441 cctgggtgat aacctgaacg cttagcacat cccccggcct gagctggtag ggtgcaggct
     1501 gcgttgaagt taaaagtccg gtgtctaagt tgttcaggta gagcagctga gccttacggt
     1561 tgcacgatac ctgcatcaag cttgcaaaaa ctataatcaa aactgctgga cgtgaaattc
     1621 tcatgttgtt gcatttcctt gcacaaaagt aacaattatt ttaaagtttt gattagtagg
     1681 catgtttatt gctggaagtt ttttgtttaa gcctggtggg gggtaacggg gttacgatgc
     1741 ctcaatttta ttagtaatgt taaccctcag gaacctataa atctgccatg atgaaatcta
     1801 atagtttggt agagctggct gtatggtttg agaaattcaa catcaagtta ggttcatagt
     1861 aggatggtaa atgttgaaat tatgcaacct gcattatctg gcaaccatcg gtagaattga
     1921 gctgacagtt gtttgctgcc tttcgaacat aatagtattt tgtttttagg aggattagtt
     1981 ttttcaaatt tgcatggtga ttttcttttt catactttta aaaccagaaa attaacccta
     2041 tgagaaggta catccttttc gcattacttg gcataaccgt ttacacagca acggggcaac
     2101 cctataacag gctggggaac ccgttggtaa ccaattttac gcctgaggat tacgatgcaa
     2161 ctgaacagac ctggtgtatt acccaggacg acaggggtgt catgtacttt ggcacctacg
     2221 ataagggaat tctagagtat gatggaaaaa cctggagaaa aattcaggtt ccggatagca
     2281 aaccggtcaa ctccatgatt aagggtgatg atggggttat ctacgtggga acatcgggcg
     2341 attttggatt tttagaaccc acagccgatg gttcgcaacg atttgtctcc ctttcgggtt
     2401 ccattaacga caccataaaa caatcgcttt cgcatattta caaaacatac tacttcaagg
     2461 gcaacgttct tttctgttcc tgctcgtaca tttttgttta tgataagcaa ggcaagctaa
     2521 cgccggttta cctaggatat cagccagagt atgccaattt tctcaccttt gtagtgaacg
     2581 ataaagttta cattggcagc tacctgaagg gtttacgaat gctgaatagc gattggacgt
     2641 tatcaacagc tcccggaggg agtagttttg aacgcattaa tatctactcc atagtgtctt
     2701 taagcccatc ggagctgctg ctggttaccg atagtggttt ttttgtttac aaccaaaaga
     2761 ccggcagctc caaaaagatg gagaagcaaa ataattttat cgagtcgatg ttaagccagt
     2821 cggtaattcc ctatcatgcc atcaagctgc ataacgggaa tattggggtt ggaacggtgc
     2881 agtccgactg gctgagcttc tttgagctaa acgccgattc ctcctcgccc agcaccatag
     2941 taaacccaca aacagggctt caggccgctc aaattaccta tctttaccag aatggcgaat
     3001 cgccggtgtg ggccacactg ttcgacggag gaatttcccg aatagagctc cattcgccca
     3061 tccgacggtt ttcgaccgaa agcggaatca acgagctaat tctggatatt attcagttta
     3121 atggcacgct ttacgttgca accatcaacg gggtttacta tcaaacggcg gctgctaacg
     3181 gaatgtcggt atttaagccg gttgagggta tcaatggcgt tgtgtggtca ttgttaaagt
     3241 tcactcctcc gggaggctcg ccaatgctac tcgccggttc gtatgttgat ggggtttttg
     3301 aaattaaggg caacagggct atttcgataa gcaaacccct ttaccgttct tttccaaacg
     3361 tgcagcacca gtgtcttacc ctttaccagt ccgtacttca tcctaacatg ctatacgttg
     3421 ggggtatttc gaggcttacc tacattgaat ggagcaaggg ggaatgggtg aaaacccaga
     3481 acatagccct tgatgagatt cgctccgaag tatggtcaat tggcgaggac gcaaggggaa
     3541 atctttggct ggggacaaac cttaagggcg tttacatgat tgatccccac gaaaaagttt
     3601 acaactttaa ctccagcagc gatttctccg gttttaagct gctacgtatt taccccgatg
     3661 gcgacagcct ttacgttctt actccaaagg gcatatttta ctatagctac agcgatagca
     3721 ccttcaggaa atgtggattg ttgggtaaaa attctttgga aaacggcttt tcacgtttgt
     3781 tgaaggtggg gaatgaaatt gttgccgcct gttacaacga ttctactggc aagtactggg
     3841 tgtgcacgat ggccaagaat ttatccggcg aatgggttac caatgacaa
//
LOCUS       DAOPHX010000185         3861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_32229_length_3861_cov_6.466369, whole genome shotgun sequence.
ACCESSION   DAOPHX010000185 DAOPHX010000000
VERSION     DAOPHX010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32229_length_3861_cov_6.466369"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..1351
                     /locus_tag="PK502_10250"
     CDS             359..1351
                     /locus_tag="PK502_10250"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPH01238.1"
                     /translation="MKVAVVGATGLVGQVMLRVLEQRNFPVTELIPVASEKSVGKTLK
                     FKNEALKVSSLEDAVRQKPSLALFSAGSAVSGEWAPRFAEEGTTVIDNSSCWRMHNEI
                     PLVVPEVNASVLSSSSKIIANPNCSTIQLVVAVNPLHQHFRVKRIVVSTYQSVTGTGV
                     KAVNQLFAERQGIATDMAYPHPIDLNCIPHGGSFLDGGYTTEEQKLIDETRKIIGDPH
                     IMVSPTVVRVPVVGGHSESVNIEFEKDFLLEQVYDILKNSPGVTVQDVPSENTYPMPR
                     MAQGRDDVFVGRIRRDVSKPHCLNLWVVSDNLRKGAATNAVQIAEYLLLQNWLK"
     gene            complement(1419..2039)
                     /locus_tag="PK502_10255"
     CDS             complement(1419..2039)
                     /locus_tag="PK502_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPH01239.1"
                     /translation="MAELVARITAWYMGNINYTTITILMAIESSFIPFPSEIVVPPAA
                     FKAAQGELNIYLVILSSTFGAIIGAIFNYFISIWIGRKLIYAFAETKFAHLLMIEPKS
                     IQKAESYFVKHGNSSTFIGRLVPGIRQLISIPAGLARMHFGKFILYTTLGAGLWNIVL
                     ALLGYYIPADLVDRYYKELSWAMIILGAAFVLRLGYKAFGKKRQPT"
     gene            complement(2080..3306)
                     /gene="hflX"
                     /locus_tag="PK502_10260"
     CDS             complement(2080..3306)
                     /gene="hflX"
                     /locus_tag="PK502_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPH01240.1"
                     /translation="MGKIFSTEKKREKAILVGVIKQGQEETEIKENLVELDFLAETAG
                     ALSIKQFIQRIESPNPSTYIGKGKVIEVLAFISDNEVDMVIFDDELSPTQLRNLEAEL
                     GIKVLDRTNLILDVFARRAKTAYAKTQVELAQYQYLLPRLAGRWTHLERQRGGIGLRG
                     PGETEIETDRRVIRNRIARLKEELGRIDKQMAIQRSNRGELVRVALVGYTNVGKSTIM
                     NLISKSDVFAENKLFATLDTTVRKVVIGNLPFLLSDTVGFIRKLPHQLVEAFKSTLDE
                     VREADLLLHVIDISHPGFEEQINVVNQTLVEIGASDKPTILVFNKIDAYSWNEKGEDD
                     LLPPDKFNTSLYSLQNSWMAKANVQTIFISARLKTNLDQFKKLLYSSVRNIHVGRYPF
                     DNFLYPGDITFDGPKS"
     gene            3625..3698
                     /locus_tag="PK502_10265"
     tRNA            3625..3698
                     /locus_tag="PK502_10265"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3659..3661,aa:Ala,seq:ggc)
BASE COUNT         1141 a          840 c          789 g         1091 t
ORIGIN      
        1 ataaccatgc cgcacaagca tcctccacgt tacatctcca ttgttaacat tccaacttgg
       61 aagattgacc atgatggtat cagtggacga gccggtattg ccaaatgtat gctttaagga
      121 aaattctcct gataacggat ttatggtaga agcctgccat cgtccagctg gtttttgtat
      181 ccaacccgaa atatcagcat tctcaaagtc atagaaaatt tgaccactaa cctcataaaa
      241 aatcaacagg ctaaaaattg ataccaccaa ccgcttcatt gtactattta gctattttta
      301 tgcggattaa tttacgaaaa aaacagcagt aattcaatca gtattaaaaa tcacacctat
      361 gaaggtagca gtagtgggcg caaccggact ggttggtcag gttatgctta gagttttgga
      421 acaaagaaat tttcctgtaa cagagttaat tcccgtagca tccgaaaaat ctgtaggtaa
      481 aactttaaag tttaaaaatg aagccttaaa ggtaagttca ttggaagatg ctgtaaggca
      541 aaagccctcc ttggcgctct tttctgccgg ctcagcggtt tcgggtgaat gggcgccaag
      601 gtttgccgag gaaggaacaa cagtaattga caactcctca tgctggcgta tgcacaatga
      661 aattccactt gttgttcccg aggttaacgc ttcagtgctg agctcttcat ctaaaattat
      721 tgccaaccca aactgttcca ccattcagct ggtagtggcg gtgaacccgc ttcatcaaca
      781 ctttagagta aaacgaatag ttgtgtcaac ctaccaatct gttactggaa ctggtgttaa
      841 agctgttaac cagctctttg ctgagcgaca gggcattgct acagacatgg cctacccgca
      901 tcccatcgac ctgaactgca ttccacacgg aggatctttt cttgatggag gctacaccac
      961 tgaggagcag aagttgatag atgaaacacg aaaaattatt ggagatcctc atataatggt
     1021 ttctcctaca gttgttcgcg ttcctgtagt aggaggacat tccgaatcgg ttaatattga
     1081 gtttgaaaaa gattttttgc ttgaacaggt gtatgacatt cttaaaaatt caccgggggt
     1141 aaccgttcag gatgtcccat cggagaatac ctatcctatg cctcgaatgg cccagggcag
     1201 ggatgatgtt tttgttggca gaattcgcag ggatgtatca aaaccccatt gtttgaacct
     1261 atgggtggtt tcagataacc tacgtaaggg ggcagcaaca aatgctgttc aaattgcgga
     1321 atacctattg ctacaaaact ggttaaaata gttctcaaaa taggtactcc gcaaaccgta
     1381 tcaatatttg ttctgtacca ataggttaat acaaaaaatt aggttggttg cctttttttt
     1441 ccgaatgcct tgtaccctaa gcgcaacaca aatgcagccc ccagtattat catggcccag
     1501 ctaagctcct tatagtacct gtcaaccaaa tcggcgggga tatagtaacc aagaagggca
     1561 agtacaatgt tccagagtcc ggcacccaga gttgtgtaaa ggatgaactt tccaaaatgc
     1621 attctggcaa gccctgcagg aatggagata agctgtcgaa tcccgggaac taaccgtcca
     1681 ataaacgtag agctgttgcc atgcttaaca aaatatgatt cagccttttg aatagattta
     1741 ggttcaatca tcaacaagtg ggcaaacttg gtttcagcga acgcatagat aagcttacgt
     1801 cctatccaga tagatataaa atagttaaaa atagcgccaa taattgcccc aaatgttgag
     1861 gataaaataa ccaggtatat attaagctct ccctgggccg ccttgaaggc cgcaggaggt
     1921 acaactatct cggaaggaaa gggaataaaa gaactttcaa tagccataag tatggtaatt
     1981 gtggtatagt ttatattccc catataccaa gctgtaattc tagcaacaag ttctgccata
     2041 gttttttttg tgaattagct tacatttcta gtgcaatgtt caacttttcg gcccgtcaaa
     2101 ggtaatatcc ccaggatata aaaagttatc gaaggggtat cgtccaacat gaatgttcct
     2161 taccgaactg tatagaagct ttttaaattg atcgaggttg gttttcaacc gtgctgaaat
     2221 gaaaatagtc tgaacattgg ctttggccat ccagctgttc tgaaggctgt aaagactagt
     2281 attaaattta tcaggaggta gtaaatcgtc ttcacccttc tcgttccagc tatacgcatc
     2341 aattttgtta aataccagaa tggttggttt gtcgcttgcc ccaatttcta ccaatgtttg
     2401 gttaaccaca ttaatttgtt cctcaaagcc cggatgggag atgtctataa cgtgtagcag
     2461 caaatcggcc tccctcacct catcgagagt tgacttaaac gcctcaacca gctgatgggg
     2521 taatttccgt ataaaaccta ccgtatcgga caacaagaat gggagatttc cgataacaac
     2581 cttacgaaca gtagtatcaa gcgtggcgaa gagcttattt tcagcaaaaa catcggattt
     2641 gctgataagg ttcatgattg ttgatttgcc cacgttggta tatccaacaa gcgcaacacg
     2701 caccaactct cccctgttgc tccgttgaat ggccatctgt ttatcaatcc ggcccagctc
     2761 ttccttaaga cgagcaatac gattgcgaat aacacgccta tcggtttcaa tttcagtttc
     2821 acccggcccc cttagcccaa taccgccacg ctgccgctcg aggtgggtcc agcgccctgc
     2881 caatctaggt aataggtatt gatactgtgc aagttcaacc tgggttttcg cataggctgt
     2941 tttagcccgt cgtgcaaaaa catcaagtat aagattcgtt ctatcaagaa ccttaatacc
     3001 aagttctgcc tccaggttcc tgagctgtgt tggggataac tcgtcgtcaa aaatcaccat
     3061 atccacttcg ttatccgaaa tgaatgcgag cacttcaata acttttccct taccaatgta
     3121 cgtgctggga tttggtgatt cgatgcgttg aataaactgt tttatggaca acgcaccagc
     3181 tgtttcagct aaaaaatcca actcaaccaa gttttccttt atctctgtct cctcctgccc
     3241 ctgcttaatt acacctacaa gaatggcctt ctcccttttt ttctcagttg aaaaaatttt
     3301 ccccatattt aaagttagcc ttgttaacgg ctggctttag ttttttttta cccagattct
     3361 actatatagt cgcacaaaaa taatatgaaa tatctaaccg agggagttat tgcaaaaaac
     3421 cgttgtgttt tagaaccaaa cattaagcta aattttcaga aaatcggaag tatcggagtg
     3481 catctttaat gtttgaatat cggcaaaaag actaattaat gttaaaggta actctaaaat
     3541 agtggatttc atcaaacatt tagcaagttg gaaattttat aaggtgatag tattttaaaa
     3601 aaaaactata tttgcgcaag aaatgggggt atagctcagt tggctagagc gcttgcatgg
     3661 catgcaagag gtcaggggtt cgactcccct tacctccacc tcattaagaa aagcccgtac
     3721 atccaagaag tacgggtttt tttattgatg ccctttacag caaaaaaact acgggtggaa
     3781 taccctgtag cctgaactcc tgagcaggaa acgggactcg aacccgtgat ccgccggtgg
     3841 gcggactgcg ctcctcagct t
//
LOCUS       DAOPHX010000186         3734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_33769_length_3734_cov_5.064148, whole genome shotgun sequence.
ACCESSION   DAOPHX010000186 DAOPHX010000000
VERSION     DAOPHX010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33769_length_3734_cov_5.064148"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            417..2987
                     /locus_tag="PK502_10270"
     CDS             417..2987
                     /locus_tag="PK502_10270"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HPH01241.1"
                     /translation="METKKDTLMNNQAEQMAATYSFDEAYEASIKYFKGDEMAAKVWV
                     SKYALKDSFGKIFEKTPDDMHRRIARELARIEARYPNPMGEEALYELLKDFRYIVPQG
                     GPMTGIGNKYQIASLSNCFVIGNSGNADSYGGIMKIDEEQVQLMKRRGGVGHDLSHIR
                     PKGSPVLNSALTSTGIVPFMERYSNSTREVAQDGRRGALMLSISIKHPDSEKFIDAKM
                     EPGKITGANVSVKIDDEFMRAAIEGKPYIQQYPVDSPKPKYRQEIDAASLWKKVIHNA
                     WQSAEPGVLFWDTIIRESVPDSYADLGYRTVSTNPCGEIPLCPYDSCRLLAINLYSYV
                     ENPFTPKAKFNFDLFKRHAHAAQRIMDDIIDLELEKIDAILDKINSDPEEEEIKAVER
                     NLWQKIRRKTIEGRRTGIGITAEGDMLAALNLRYGSDNAVDFAVEVQRVLAVEAYRSS
                     VNLAKDRGAFPIYNAQRETNNPFIKRIKAEDEQLYSEMVTYGRRNIALLTIAPTGTTS
                     LMTQTTSGIEPVFLPYYRRRRKVNPNDKNVKITFVDGVGDSWEEFNVFHHKFLTWLEV
                     NGYNTDEVKGYSDDKLQELVKLSPYYGATSGDVDWVSKVKMQGMIQKWVDHSISVTVN
                     LPENATEELVAKVYQTAWESGCKGCTVYREGSREGVLIADRKDAEGDSKDTFPAKRPP
                     VLDCDVIRFKNNREDWVAFVGLYDNRPYEIFTGLTEDDVLPIPKSVIKGKIIKIKDEN
                     GSRYDFQYVDKYGYTNTIGGLSHMFNKEYWNYAKLISGVLRNGMPIPDVVNLVSSLRL
                     DSETINTWKAGVERALRRYIPDGTKAKPGKKCPECGSESLIYQEGCLTCQSCGYSKCG
                     "
     gene            complement(3047..3553)
                     /locus_tag="PK502_10275"
     CDS             complement(3047..3553)
                     /locus_tag="PK502_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HPH01242.1"
                     /translation="MTSCMQLRGDPLVYETLQRLNISFDYYEHPPVPTVEEATKYWKG
                     IDSTHCKNLFLRNHKGDRHYLVILEYTQVLNIHQLERVLNQGKLTFASPQRLLKYLGL
                     TPGSVSPFGLLHDTSHHVQVFIDSQLCQTHHLSFHPNLNTASLVIPTRDFFIFLAWTG
                     NPYELITI"
BASE COUNT         1039 a          806 c          946 g          943 t
ORIGIN      
        1 aagttccgcc agtaggctga caggtttgaa ccctgagcct gtcgaagggt gaatccgcca
       61 gtaggcggac agatttgaac cctgagcctg tcgaagggtg aatccgcaag taggcggaca
      121 agttcttgaa tcctgcttga atttttttat cctttatcct tgtcttcagc cccaacgggg
      181 cgcaatagct agcaaggggt gaagtccctt gtcagctttt attgccttcc cttcataagc
      241 tgccggtgtt aataactaaa aatgattgtt aatatttaga acatgtttag cttggcgtca
      301 aaggttaacc tttaataact tcgtacctgc tttcccaaat tttcaaaaat ccgcacaatt
      361 cttattgtca ggcatatggg aaagtttttt attgcgactt atacaaccaa aatataatgg
      421 agacaaaaaa agataccctg atgaacaatc aggcagaaca aatggcagca acctatagtt
      481 ttgatgaagc ctacgaggca agtattaagt attttaaggg tgacgaaatg gctgcaaagg
      541 tttgggttag caagtatgcc cttaaggatt cctttggtaa aattttcgaa aagacacccg
      601 acgacatgca tcgtagaatt gcccgtgagt tggcaagaat cgaggcaaga tacccaaacc
      661 cgatggggga ggaagcgctc tacgagctgc taaaagattt tcggtacata gtaccccagg
      721 gagggccaat gaccggcata ggcaataagt atcaaattgc ctcgctttct aactgctttg
      781 taatagggaa tagcggtaat gccgattcgt atgggggaat tatgaaaatt gatgaggagc
      841 aggtgcagct tatgaagcgc aggggaggtg taggccacga cctttcccac atccgaccaa
      901 aaggaagccc tgtgcttaac agtgcgctaa cctctacagg tattgtgccc ttcatggagc
      961 gctactcaaa ctccacgcgc gaggtggctc aggacgggcg gcgcggagct ctaatgctaa
     1021 gcatctccat aaaacatcct gattccgaaa aatttataga tgccaagatg gagcccggga
     1081 aaattacggg tgctaacgtg tcggttaaaa ttgacgatga gtttatgcgg gcagctattg
     1141 agggtaaacc ttacattcaa cagtaccccg ttgactcacc taagccaaag taccgccagg
     1201 aaattgatgc tgcatccctg tggaaaaagg tgatacacaa cgcatggcaa tcagctgaac
     1261 cgggggttct attctgggat accattattc gcgaatcggt tcccgacagc tatgccgatt
     1321 taggttatcg aaccgtatca accaatccct gtggcgagat tcccctctgc ccttacgata
     1381 gctgccggtt gctggcaatc aacctttata gctatgttga aaatcccttt acccctaagg
     1441 caaaatttaa cttcgacctg ttcaaaaggc atgcccatgc agcccaacgc atcatggacg
     1501 acatcattga cctggagctg gaaaagattg atgccattct cgataaaatt aactccgacc
     1561 ccgaagaaga agaaattaag gctgttgaac ggaacctctg gcagaaaatt aggcgcaaga
     1621 caattgaggg aaggcgaact ggaattggta ttacggccga aggagatatg ctggctgcac
     1681 taaacttgcg ctacggctcc gataatgcag ttgattttgc tgttgaagtt caacgtgtgc
     1741 tagctgttga ggcataccgt tcgtctgtaa acctggctaa ggatagggga gcattcccta
     1801 tttataacgc ccagcgtgaa accaacaacc cttttattaa gcgtattaag gccgaagatg
     1861 agcagcttta tagcgaaatg gttacctatg gcaggagaaa tattgccctg ctcaccattg
     1921 cacctaccgg cacaaccagc cttatgaccc aaacaacctc tggaatagag ccggtatttc
     1981 tgccctacta cagacgacgc cggaaggtga accccaacga taaaaatgtt aaaataacct
     2041 ttgttgatgg ggtgggggat tcatgggagg aatttaacgt gttccatcat aaatttctta
     2101 catggctgga ggtaaacggc tacaacaccg atgaggttaa aggttacagc gacgacaagc
     2161 ttcaggaact ggtaaaacta tcaccctact atggcgctac ttctggagat gtggattggg
     2221 taagcaaggt taaaatgcag gggatgatac agaaatgggt tgaccactcc attagcgtaa
     2281 ccgtcaacct gcccgagaat gccaccgaag agctggtggc caaagtttac caaactgctt
     2341 gggaaagcgg ttgcaagggt tgcactgtct atcgtgaggg atcgcgtgag ggcgttctga
     2401 ttgccgatag aaaggatgcc gaaggggatt caaaagatac cttccctgct aaaaggccac
     2461 ccgtgctcga ctgcgatgta attagattca aaaataaccg tgaggactgg gtggcatttg
     2521 ttggcttgta tgacaaccgc ccctacgaga tttttaccgg tttaaccgag gatgacgttc
     2581 tcccaatccc caaatcggtg atcaaaggga aaattatcaa gataaaggat gagaatggaa
     2641 gccggtacga ctttcagtat gttgataagt atggctatac caatacgatt ggcggcctgt
     2701 cgcacatgtt caacaaggag tactggaact atgccaagtt gatttcaggt gtgctgcgca
     2761 acggaatgcc cattcccgat gtggttaatc ttgtgtcgtc gctgcggctg gatagcgaaa
     2821 caattaacac atggaaggca ggagtggaga gagccctacg tcgctacatt cctgatggca
     2881 ccaaggccaa acccggcaaa aaatgccccg agtgcggttc cgaatcgcta atttaccagg
     2941 agggatgttt gacatgtcaa agttgtgggt attccaaatg cggctaagtt gtcacgtgta
     3001 gctatttaag ctcgtgttcc ccttgctagt gtgcaggggg aaatttttat atggtgataa
     3061 gctcgtaggg gttacctgtc catgccagaa agataaaaaa atcccttgtt gggattacca
     3121 gggaggcggt gtttaggtta gggtgaaagc tgaggtggtg agtctggcac agctgagaat
     3181 caataaacac ctgcacatgg tgtgaggtgt cgtggagtag tccgaaaggt gaaaccgagc
     3241 ctggagttag ccctaggtat ttgagtaagc gttggggtga tgcaaacgtt agttttcctt
     3301 gatttagcac cctttcgagc tggtgaatgt taagaacttg tgtgtattca aggattacca
     3361 ggtagtgcct atcgccctta tgattccgaa gaaataggtt tttacaatgg gtggaatcaa
     3421 tacccttcca gtattttgtt gcctcctcaa cggttggcac cggagggtgc tcatagtaat
     3481 caaaagaaat gttaagcctt tgcagggttt cgtatacaag cggatcgcct cgaagctgca
     3541 tgcacgatgt cattggtggt ggaccttcta aattataata ttctgcagcc tgctattgat
     3601 cgcctccatg ttaaaggttt cgggattgta gtcctcgcca agccattcgg caattaatgg
     3661 gtaccgccat tccttgttct ggtgcagctg aagcatatcc aaggtatcgg tgtagcaaac
     3721 aatacctccc gatt
//
LOCUS       DAOPHX010000187         3655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_34784_length_3655_cov_5.965278, whole genome shotgun sequence.
ACCESSION   DAOPHX010000187 DAOPHX010000000
VERSION     DAOPHX010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34784_length_3655_cov_5.965278"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3441
                     /locus_tag="PK502_10280"
     CDS             <1..3441
                     /locus_tag="PK502_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPH01243.1"
                     /translation="MENQTNPEEFYRLLAISKERMKELAAINNLVSIIKENKPIPETL
                     YQLCLVLADAWQYPEYTAVRIRYGQYEFQTRNFRETPWSQKEEFESIDGIRGLLEVYY
                     TQKFPEADEGPFLKEERDLIKNLSNIIVGYINSVKGQEVLKDKAKPKRRGFEVEPQSK
                     SLLQRFINQQNVDRDIYHDLMPFKVHEILLISTLYDAYSIEKEDRLTDNILGEYTKLS
                     LSSVPRITGVSNLDEALDKLEERYFNMIIIMMGADTVAPLKMAQRIKGEYNHIPIYLL
                     VNNSVIVDEMEKNPVAITGIDRMFVWNGEPKIFFSMIKLLEDRVNIENDTRIALIRLI
                     LLVEDSPKYYSRYLPLLYGSVLDQTKRMIEDVSTDELYKVLRIRIRPKILLAENYEEA
                     MELFSRYKDFMLCVISDVKFFRKGKLDEHAGVSLVEQVRKELPNLPIILQSFDRSNEE
                     TALKLKVAFLDKNSESLMLDIKNFLSNYLGFGDFVFKDSEGNPITIASTMEEFEKALR
                     IIPDESLLYHAQKNHFSMWLAARGEIQVARIIHPSRIDDFSGPDEIREYLILSLRKYR
                     QEKRRGKIVTFDSDWMVDESNIVSLADGSFGGKGRGLSFINTLLYTFDVSQYTPNINL
                     HTPRTSIIGTNEFENFMANNSLYEVVFSAKSYDEIKRYFLKAELSNQLKIKLDRLLQI
                     YYRPLAIRSSGMLEDSIMQPFAGIFDTYMIPNAHADRSIRLQSLMNAIKLVYASVFSP
                     TARAYVKAINFKIEDEKMAVIVQEVVGNKFENYYYPHISGVAQSYNYYPFGHINPEDG
                     FANIAVGLGKHVVEGGKSYRFCPRYPTLINYTLEDLVKNSQTDFLAIDLSRPDFDLLS
                     GDEAALARLDMSYAEQHGNLKHCASVYSSNNNSLIAGIGQPGPRVINFPNILKYNYIP
                     LSQTIDVILDIVKEAFGSPCEIEFAVDLNKDANFKASFFLLQIKPLIGNYNEYKVNLD
                     ELDLKSVILLSNTGMGNGSISNLYDVVYIKRNVFDKSMTPTMALEVEAINEKLATEGK
                     NFILIGPGRWGTRDRWIGIPVTWPQISNAKVIVETSLDDFPLDASSGSHFFHNVISMN
                     VGYCSVPNNDSFARIAWSKLDELPAYNETKYFRHVRFPKPLQVKMDGTQRLIVVTHEP
                     "
BASE COUNT         1015 a          808 c          868 g          964 t
ORIGIN      
        1 atggaaaatc aaactaatcc cgaggaattc taccgcctgc tcgccatatc gaaagagcgg
       61 atgaaggagt tggctgcaat taacaacttg gtttccataa taaaggagaa caagccaatt
      121 ccagaaacgc tttaccagct ttgtcttgtt ttagcagatg cttggcagta ccccgagtac
      181 acagcagtaa ggattaggta tgggcagtat gagtttcaaa cccgtaactt tagggaaacg
      241 ccttggagcc aaaaggagga gtttgaatct attgatggaa taaggggttt actggaggtt
      301 tactacaccc aaaaatttcc ggaagctgat gaaggccctt ttctgaagga agagcgcgac
      361 ctgattaaaa acctatccaa cataatagtt ggctatatta acagcgtaaa gggtcaggaa
      421 gttcttaaag ataaggctaa gccaaaacgt agaggttttg aggttgagcc ccaaagcaaa
      481 agcctgcttc agcgatttat caaccagcag aatgtagata gggatatcta ccacgatttg
      541 atgcccttta aggtgcatga gattttgctt atttccacgc tgtacgacgc ctacagcatt
      601 gagaaggagg accggctgac ggacaatatt ctaggagagt acacaaagtt gagcctttcc
      661 agcgtgcccc gaattacagg ggtttccaat cttgatgagg ctcttgataa gctggaggag
      721 cgctacttta acatgattat catcatgatg ggcgccgata cggttgcccc cctaaagatg
      781 gcccagcgca ttaagggcga gtacaaccat attccaattt acctgctggt aaataatagc
      841 gtgattgttg atgagatgga gaaaaatcct gttgccatta ccggcatcga caggatgttt
      901 gtgtggaacg gggaaccaaa gatttttttc tccatgatca agctactcga ggatagggtc
      961 aatattgaga atgatacccg tattgcgctc ataaggttaa tcctgttggt tgaggactcc
     1021 ccaaaatact actcccgata cctgcctcta ctctatggta gtgtacttga ccaaaccaaa
     1081 aggatgatcg aggacgttag caccgacgag ctttacaagg tattacggat taggattcga
     1141 ccaaaaatac tcctggccga gaactacgag gaggccatgg agctgttctc acgttacaag
     1201 gatttcatgc tttgcgtgat ttccgatgtc aagttcttta ggaaggggaa gctggacgag
     1261 catgctgggg tgagcctggt tgagcaggtg cgtaaggagt tgcccaattt gcccattatt
     1321 ctgcagtcgt tcgacaggtc caatgaggaa accgcgctta agctcaaggt ggcatttttg
     1381 gacaagaatt cggagagcct gatgctcgac atcaagaatt tcctgagcaa ctacctggga
     1441 tttggcgatt ttgtgtttaa ggatagcgag ggcaacccca ttaccattgc ctccaccatg
     1501 gaggagtttg agaaggctct gaggataata cctgacgaga gccttttgta tcatgcccag
     1561 aagaatcact tttccatgtg gctggccgcc aggggtgaaa ttcaagttgc acgaatcata
     1621 catccttcaa gaatagacga tttctcgggt cccgatgaaa ttcgcgagta ccttattttg
     1681 tccctcagga agtacaggca ggaaaagcgt agagggaaga tagttacctt tgatagcgat
     1741 tggatggttg atgaaagcaa cattgttagc ctggccgatg gttctttcgg gggaaagggg
     1801 aggggattgt cgtttatcaa caccctgctc tacacgtttg atgtttcgca gtacacccca
     1861 aacatcaacc tgcacacccc aagaacatcc attatcggca caaacgagtt tgagaacttc
     1921 atggctaaca atagccttta cgaggtggtt ttcagcgcta aaagctacga tgagattaaa
     1981 cggtattttc tgaaggcgga gctatcaaat caactgaaaa tcaagttgga caggttgctg
     2041 cagatttact ataggccact tgccatccga tcttcaggta tgttggaaga ctccattatg
     2101 cagccttttg cgggtatttt tgacacctac atgattccta atgcccatgc cgaccgctca
     2161 atccgacttc aaagcctgat gaacgccata aagctggttt acgcctccgt tttctcaccc
     2221 acagcccgtg cctatgttaa ggccattaac tttaagattg aagatgaaaa gatggccgtt
     2281 attgttcagg aggtggtggg aaacaaattc gaaaactact actatcccca tatcagcggg
     2341 gttgcacaat catacaacta ctaccccttt gggcacatta accccgaaga tggatttgcc
     2401 aatatagccg tggggcttgg caagcatgtg gttgaaggag gaaaatccta tcggttttgc
     2461 ccccggtacc caaccctaat taactacacc ctggaggacc tggtaaaaaa ttctcaaacc
     2521 gattttctcg ccattgacct cagcaggcca gacttcgact tgctttcagg tgacgaggct
     2581 gcccttgcgc gtctggatat gagctatgcc gagcaacatg gtaatttaaa gcattgtgca
     2641 tcggtttata gctccaacaa taatagcttg attgccggaa tcgggcaacc gggtccgagg
     2701 gttatcaatt tccctaatat tctcaagtac aactatattc ctctttccca aacaattgat
     2761 gtaatcctcg acattgtaaa ggaagccttt ggctccccct gcgaaataga gtttgctgtt
     2821 gatctgaata aggatgccaa tttcaaggct tcattcttcc tgcttcaaat taaacctctt
     2881 attggcaact acaacgagta taaggtgaac ctggatgagc tcgatttgaa gagtgtcata
     2941 ctcctttcaa atacaggaat gggtaacggc tcaattagta acctttacga tgtggtgtac
     3001 atcaaacgaa atgtttttga taagtccatg accccgacaa tggctttaga ggttgaggct
     3061 attaacgaaa agctggctac tgagggaaaa aatttcattc tcattggccc cggaaggtgg
     3121 ggaacccgcg ataggtggat tggcattccc gttacttggc cacaaatttc caatgccaaa
     3181 gtaattgtcg agactagctt ggatgatttt cccctcgatg cctcatccgg ctcccacttt
     3241 tttcacaatg ttatttccat gaacgtggga tactgctctg ttcctaacaa cgatagcttc
     3301 gcacgaattg catggagtaa gctcgatgag ctacctgctt acaacgaaac taaatatttc
     3361 aggcatgtcc gctttcctaa acctctgcag gttaaaatgg atggcacgca acgtttgatt
     3421 gtggttactc atgaaccctg agtatgctgg agcgtctagc tggaagcttc tacagttaaa
     3481 gaaaaaaata attccagatt caggaacttg tccgccagta ggcggacagg ttccgtcagg
     3541 aaccggacaa gttcttgaat ccgccggtag gcggacaagt tccgtcagga gccggacaag
     3601 ttccgccagt aggcggacag gttcttgaat cctgcttgaa tttttttatc cttta
//
LOCUS       DAOPHX010000188         3644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_34937_length_3644_cov_6.342435, whole genome shotgun sequence.
ACCESSION   DAOPHX010000188 DAOPHX010000000
VERSION     DAOPHX010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34937_length_3644_cov_6.342435"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PK502_10285"
     CDS             <1..873
                     /locus_tag="PK502_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPH01244.1"
                     /translation="ANIALTLILLLMISLMGMNAAYVSWCHSLGKEIHGVYPVSQHLT
                     GLFSSMPVEVIYTYYEVFWWSHILLIFLFANILPYSKHFHVFMSIPNVFLSRLEPLGK
                     LPSMESITREVKLMLDPNAAFAAPPSDTPIERFGVKDAEDVVWKSYFDSLSCTECGRC
                     TSVCPANLTGKRLSPRKVMMDLRARMKEKGPLMLKNGRDYSDGKSLIRDYISEEELWA
                     CTTCNACAQECPININHPTLIVDMRRYLVMEEGSAPGEIKAMFNNIENNGAPWQYSPE
                     DRLNWANNLEINTQ"
     gene            927..1721
                     /locus_tag="PK502_10290"
     CDS             927..1721
                     /locus_tag="PK502_10290"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPH01245.1"
                     /translation="METKKIEVPVMAELFARGEKPEYLFWVGCAGAYDDRYKKVTRAF
                     TKILVHLNISFAVLGKEETCTGDPARRAGNEMLFQMQAFQNIELFKSYDIKKIITICP
                     HCFNTFKNEYPDLGGSYEVISYTQLLEQHITEGKLKLDAGKFKNTRITYHDPCYLGRA
                     NGIYEEPRRIIKTLTGEFVEMERNRSFSLCCGAGGAQMFKEAEKGNKEVFIERTEDAL
                     KVNPEIIATACPFCMTMITDGIKYKNQEEKIRNLDIAELIAESLNL"
     gene            1748..3526
                     /locus_tag="PK502_10295"
     CDS             1748..3526
                     /locus_tag="PK502_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPH01246.1"
                     /translation="MSESKLLKSGEFLVNEVEANDIFIPEEFNEEQRMIAQTCRDFLE
                     AEVYPKLDDLDKSDRELMKSLLKKTGELGLLGIAVPEEYGGFGQSFITQMLVAETTGA
                     GYSFSVAYMAHCGIGTMPILYYGNEEQRQKYVTRLATGELLGAYCLTEPGAGSDANSG
                     KTNAKLSEDGKHYILNGQKMWITNAGFADTQVVFAKIENDRVLSAFIVESKWPGVVIG
                     PDEHKMGIKGSSTAQIYYNDVKVPVENLLGNRGEGFRIALSILHMGRMKLGANVIGAA
                     KETITQSVRYANERKQFNTQIANFGSIKHKLAEQVIRTFAHESAIYRVSQNIDELITR
                     YKAEGCDYGKAAIDAISHYAVEDAILKVNGSEMLDFVVDEGVQIHGGMGYSAEMNVER
                     GYRDSRINRIFEGTNEINRLLVVDTAIKRSLKGDFNLMGPAEELYTNLDGIKVEMGAA
                     GYFEQKKQLIRNFKKIALLGIFGANKTFAKQFVSEQEVQNNLSNIIMDIYVAESLALR
                     VEKLESRGHASMDIYRSILDVFIFDAASRIRKNAIDAISSFAAEPELGKLIAATERLS
                     AVNPINVKEARRKIADKLIADNVYKF"
BASE COUNT         1068 a          839 c          878 g          859 t
ORIGIN      
        1 gccaacatcg cccttacgct aattttgctt ttaatgattt cccttatggg gatgaacgct
       61 gcctacgtga gctggtgcca ttctcttgga aaggagattc atggagttta tccggtaagc
      121 cagcacctta ccggtctgtt ttcctccatg ccggtagagg tcatttacac ttactacgaa
      181 gtcttttggt ggtcacacat tctgctcatt tttctttttg ccaacatact cccctactcc
      241 aagcactttc atgtgttcat gtccatccct aacgtatttc tgtcgaggct ggaaccactg
      301 ggcaagctac ccagcatgga gagcattacc cgcgaggtta agcttatgct ggatccgaac
      361 gctgcctttg ccgcacctcc ttccgacaca ccaatcgaaa gatttggcgt aaaggatgcc
      421 gaggatgtcg tttggaaaag ctacttcgat tccctctcct gcaccgaatg cggacgctgc
      481 acctccgttt gcccggcaaa cctaaccggc aaaaggcttt caccccgcaa ggtaatgatg
      541 gacctgcgcg caaggatgaa ggagaaaggc ccattaatgc tgaaaaatgg aagggattac
      601 agcgatggca aatcgctgat tagggactac atctccgagg aggagctctg ggcttgtact
      661 acctgcaacg cctgcgccca ggagtgtcca atcaacatta accatcccac cttgattgtg
      721 gatatgcgcc gctatctggt catggaggag ggctcagcac ccggagaaat aaaggcaatg
      781 tttaacaata tcgagaacaa cggagcaccc tggcagtact caccagaaga caggcttaac
      841 tgggctaaca atttggaaat caacacacaa taagtatttt tcccattaca atgcaaacac
      901 aaccatttga ataaccagcc ataaagatgg aaacaaaaaa aatagaagtg cctgtaatgg
      961 ccgaactttt cgcccgcgga gaaaaacccg aatacctgtt ctgggttgga tgtgccggag
     1021 cctacgacga taggtacaag aaagttaccc gggcatttac caaaattctg gttcacctga
     1081 acatcagctt tgccgtgttg ggaaaagagg aaacctgcac gggtgatcct gcccgaaggg
     1141 ccggaaacga gatgttgttc caaatgcagg catttcaaaa tattgagctg ttcaagtcat
     1201 acgatattaa aaaaattatc accatctgcc ctcactgctt taacaccttt aaaaacgagt
     1261 accccgattt gggcggtagc tacgaggtta ttagctacac ccaactgctg gagcagcaca
     1321 taaccgaggg gaagctaaaa cttgatgccg gtaaattcaa gaacacccgt ataacttacc
     1381 atgacccatg ctacctgggt cgggccaacg gcatttatga agaaccccgc agaataatta
     1441 aaaccctgac tggcgaattt gttgaaatgg agcgcaaccg gagtttttcc ctttgctgcg
     1501 gtgccggagg ggcacaaatg ttcaaggagg cagagaaagg aaacaaggag gttttcatcg
     1561 agcgaaccga ggatgcgctg aaggtgaacc ccgaaatcat agcaaccgca tgtccgtttt
     1621 gcatgaccat gataacagat ggtattaagt ataagaacca ggaggagaag attcgtaacc
     1681 tcgacatagc ggagcttatt gccgaatcgt taaatctata gactacaaaa cataaaccaa
     1741 gcaaaccatg agtgagagta aactacttaa aagtggcgaa tttctggtaa acgaagttga
     1801 agccaacgat atttttatcc ctgaagagtt caacgaggag caaaggatga ttgcccaaac
     1861 atgtcgcgat tttcttgagg ctgaggttta ccccaagctt gatgacctgg ataaaagcga
     1921 cagggagctg atgaaaagct tactgaaaaa aacaggtgag ctcgggttgc tcggcatagc
     1981 agttccagaa gagtatggcg gttttggaca atcgttcatc acgcaaatgc ttgttgccga
     2041 aacaaccggt gctggctact cattttctgt ggcctacatg gcccattgcg gaattggtac
     2101 catgccaata ctatactatg gaaacgagga gcagcgccag aagtatgtaa cccgacttgc
     2161 aaccggcgag ctacttggtg catactgctt aacagaacct ggagccggca gcgatgcaaa
     2221 ctctggcaaa accaacgcta agctttctga agatggcaag cactacatac tcaacggcca
     2281 gaaaatgtgg attaccaacg ccggatttgc tgatacccag gtggtattcg caaaaattga
     2341 aaacgacagg gtgctaagcg ccttcattgt ggaaagtaag tggccgggcg ttgtgattgg
     2401 ccccgatgag cacaagatgg gaataaaagg atcctctacc gctcaaatct actataacga
     2461 tgtgaaagtt ccggtcgaaa acctgttggg aaaccgtggt gaggggttcc gaattgccct
     2521 tagcattctt cacatgggac gaatgaagct gggggctaac gttattgggg cagccaagga
     2581 aaccataacc caatcggttc gttacgctaa tgagcgcaag cagttcaaca cgcaaatagc
     2641 caactttggc tccattaagc ataagctggc cgagcaggtt atcagaacat ttgcccacga
     2701 atcggcaatc taccgggtta gtcagaacat cgacgagctg attacacgct acaaagccga
     2761 aggttgcgac tacggcaagg cagctattga tgccataagc cactacgctg ttgaggatgc
     2821 cattctgaag gtaaacggtt ccgaaatgct cgactttgta gttgatgagg gtgttcaaat
     2881 ccatggcgga atgggctact ctgccgaaat gaatgtggaa agaggctacc gcgactcccg
     2941 tatcaaccgg atttttgagg gaaccaacga aattaaccgc ctgctggtag ttgacacagc
     3001 aataaagcgc tcgctgaaag gcgacttcaa ccttatggga ccagccgagg aactttacac
     3061 caacctggat ggaattaagg tagaaatggg tgctgctggt tactttgagc agaaaaaaca
     3121 gctcatcagg aacttcaaaa agattgccct gctggggatt tttggtgcca acaaaacttt
     3181 tgcaaagcag tttgtaagcg agcaggaggt tcaaaataac ctcagcaaca tcatcatgga
     3241 tatttacgtt gccgaatcgc ttgccctaag ggtcgaaaaa ctagagagca gaggtcatgc
     3301 ttcaatggat atatacagga gtattctgga cgtttttatt tttgatgctg cctccagaat
     3361 ccgtaaaaat gccattgatg ccatatcatc ctttgctgct gagccagaac taggtaagct
     3421 gattgctgct accgaacgcc taagcgcagt aaaccccatt aatgttaagg aagcccgtag
     3481 aaaaattgcc gataagctga ttgccgataa tgtgtataag ttctaattga ggggagtaag
     3541 aatgctaaaa tcctgacatt tcatttaggc attttcaaag tcattaaaaa gggtaatcga
     3601 tttcggttac ccttttatgc atacctatgg ctgatatttc cccg
//
LOCUS       DAOPHX010000189         3501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_36946_length_3501_cov_5.021184, whole genome shotgun sequence.
ACCESSION   DAOPHX010000189 DAOPHX010000000
VERSION     DAOPHX010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36946_length_3501_cov_5.021184"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PK502_10300"
     CDS             <1..675
                     /locus_tag="PK502_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPH01247.1"
                     /translation="CIQTLRMLIEGKLKVSLQDRLFFLGDYIDRGPDSSAVVEYIIGL
                     MRSGYTISCLIGNHEQMMLDSVKGESELCLWQANSGQATIKSYVKMFGFDDDGHLGFL
                     PKAHLDFFTSLKQYFIVDDKYILVHGGLNTTAANPLEDTEAMLWSRLDDAPNDFMQDY
                     RIIHGHTPEALDVIADKLKQPNRRIFGLDAGCVYRGMLFPGTGYLVALDLDKWELNFV
                     ECLDKW"
     gene            complement(823..3054)
                     /gene="bglX"
                     /locus_tag="PK502_10305"
     CDS             complement(823..3054)
                     /gene="bglX"
                     /locus_tag="PK502_10305"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HPH01248.1"
                     /translation="MRIRKLSEITLAAIMSYTILSCSKTTMPYSDVVDQKVDSILMLM
                     TLEEKIGQLNLVTSDMDVTGPFIRKGYEEDIAKGRVGAIFNAYGAGYTRKLQELAVNN
                     TRLRIPLLFGYDVIHGHKTIFPVPIGEASSWDLEAIERSARVAAEEASAQGLHWTFAP
                     MCDIARDPRWGRVVEGSGEDPLLGSLIAQARVRGFQGTDLSQTNTILACVKHFAAYGA
                     AQAGRDYHSVDMSERQLREVYLPPYKAAIDAGALSVMSSFNDLNGIPATASKYLMTDI
                     LRNEWGFQGFVVTDYTSIMELLHHGVAADTLDAARLALNAGIDMDMQSGFYNSALLKL
                     LNEKKINEEQINQAVRRILRIKFLLGLFDDPYRYCSISREKETLLSNDNLLAARDMAK
                     KSIVLLKNQNNLLPLDKNIKTIAVVGPLANSQRDMMGSWSAAGDWPNSVTLLEGIKNK
                     IPGSQILYAQGCAIDDSNTGMIDEAVKIAKRADVVILAVGEAAWMTGEASSRASLDLP
                     GVQQELVERIYSTGKPIVAVLMNGRPLTIGWIDQHIPSILETWFLGTQAGNAIADVIF
                     GDYNPSGKLPITFPRSVGQIPIYYSMRSTGRPFDANNKYTSKYLDESNDPLYPFGYGL
                     SYTTFDYGEVELSSSEITETDTLDISVSVTNTGKYYGREMVQLYIHDNVASVAPPVKL
                     LKQFQFVDLKPGESKKMVFKLTVNDLMFYRDDMSYGFEKGQFTVYIGGNSRDVRTAFF
                     SLN"
     gene            complement(3070..>3501)
                     /locus_tag="PK502_10310"
     CDS             complement(3070..>3501)
                     /locus_tag="PK502_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HPH01249.1"
                     /translation="NVNHTLINRQYCITDPRGYLLYSPDCWGLTASDDNNGYGAHEPL
                     NDNGTITPTAALSSMPYTPEESMRVARFLYYTLGDKLWGDYGFYDAFNPTAGWWGKSY
                     LAIDQGPIIIMIENYRTGLCWNLFMSAPEVDGALSKLGFSH"
BASE COUNT          922 a          843 c          829 g          907 t
ORIGIN      
        1 tgcatacaaa cccttaggat gcttattgaa ggaaagctaa aggtatcgct acaggacagg
       61 cttttcttcc tgggcgatta cattgatagg ggccccgata gctcggcagt agtggagtac
      121 atcattgggt taatgagatc gggctacacc atcagctgcc ttattggaaa ccacgagcag
      181 atgatgcttg actcggtaaa gggagagtcg gagctatgcc tttggcaggc caactcgggt
      241 caagccacca ttaaaagcta cgttaaaatg tttggttttg atgatgatgg tcatttgggc
      301 tttcttccta aagcacattt ggactttttc acctcgctaa agcaatactt catcgttgac
      361 gataagtaca ttcttgtaca tggcgggctg aacactaccg cagccaatcc cctggaggat
      421 acagaagcca tgctctggag caggcttgac gatgccccaa acgattttat gcaggattac
      481 aggataatac atgggcatac ccccgaagcg ctagatgtga tagccgacaa actcaagcag
      541 cccaacaggc gaatttttgg gttggatgcc ggttgtgtgt acagggggat gcttttcccc
      601 ggtaccggct acctggttgc ccttgacctt gacaagtggg agcttaactt cgtagagtgc
      661 ctggataaat ggtgaaatac tcaatcaaca gctgcattca ccacgaacta atccatctca
      721 aaaaatgtag taaaatagct caccccactt actccattca ctaataaaag attaggtgga
      781 atatacttcc acctattctc caaaccaaac ctaaaacaaa ctctaattta aagaaaaaaa
      841 cgctgtcctt acatcacgcg aattaccacc aatataaaca gtgaattgtc ccttttcaaa
      901 tccgtaggac atatcatccc ggtagaacat taagtcgttt acggtaagct tgaataccat
      961 ttttttactc tcaccgggtt taaggtcaac aaattgaaat tgcttaagaa gctttacggg
     1021 tggggcaacc gaggctacat tatcatgaat gtagagctgc accatctctc gaccatagta
     1081 cttcccggta ttcgtaacac ttacggatat gtcaagagta tcggtctcgg taatttcact
     1141 ggagcttaac tcaacctccc cgtaatcgaa ggttgtgtag cttaatccgt agccaaaagg
     1201 gtagagcgga tcgttgcttt cgtcgaggta ctttgaggtg tacttattgt ttgcatcgaa
     1261 agggcgaccg gtgcttcgca tggagtagta aatggggatt tgtccaaccg aacgcgggaa
     1321 ggtaatggga agcttaccag aagggttgta gtcgccaaat atgacatcgg caatggcgtt
     1381 accggcctga gttccaagaa accatgtttc cagaattgag ggtatgtgct ggtctatcca
     1441 gccgattgtg aggggtcttc cgttcatcag cacagccaca ataggcttgc cggtgctgta
     1501 aatcctttca accagttcct gctgaacacc tggaaggtca agactggcac ggctggaggc
     1561 ctcaccggtc atccatgcag cctctcccac tgccagtata acaacatcgg cacgtttagc
     1621 aattttaacg gcctcatcaa tcataccggt gttggaatcg tctatagcac agccttgtgc
     1681 gtatagaatt tgtgaacctg gtatcttgtt cttaatgccc tcaagaaggg taaccgaatt
     1741 tggccagtca cctgcagccg accagctgcc catcatatcc ctttggctgt tagcaagggg
     1801 accaaccacc gctatggttt taatattctt gtcgagtggc aacagattat tttggttttt
     1861 cagcagcaca atggattttt tagccatatc cctagctgcc aacaggttat cattacttaa
     1921 aagggtctcc ttttccctgg agatagagca gtaccggtag gggtcgtcaa acaaaccaag
     1981 taagaatttg attcgtagaa tccttctaac tgcctggttg atttgctcct cgttgatttt
     2041 cttttcattt aaaagcttca acaaggcgct gttgtagaat cccgactgca tatccatatc
     2101 aattccggca ttgagagcca ggcgggctgc atcgagggta tcggccgcaa ctccatggtg
     2161 gagcagctcc ataattgagg tgtagtcggt taccacaaat ccctgaaaac cccactcatt
     2221 tcggaggata tcagtcataa ggtatttact ggctgtggcg gggatgccat taaggtcgtt
     2281 gaatgaggac ataaccgaaa gtgcgccggc atcaatagca gccttatatg gcggtaggta
     2341 aacttcacgc agctgtcgtt cgctcatatc aacgctatgg tagtcgcggc cagcctgagc
     2401 tgcaccataa gcagcaaaat gcttcacgca agcaagtata gtgttggttt gacttaagtc
     2461 ggttccctga aaacctctaa cccttgcctg agctatgagt gatccaagca ggggatcctc
     2521 gcccgaaccc tcaacaaccc tcccccatcg cgggtcgcgg gcaatgtcgc acatgggggc
     2581 aaaagtccag tgtaggcctt gagccgaagc ctcctcggca gctacccgag ccgaacgctc
     2641 aatggcctct aaatcccagc tggaggcctc gcctatggga actgggaata tggttttgtg
     2701 gccatgaatc acatcgtagc caaagagtaa cggaatgcgg agcctggtat tattaacagc
     2761 cagctcctga agtttacggg tgtagcctgc gccataggca ttaaatatgg ccccaactct
     2821 accctttgca atatcctcct catacccctt acgaatgaat ggaccagtaa catccatatc
     2881 gctggtaacc aggtttagct gtccgatttt ttcctcaaga gtcatcagca tcagaataga
     2941 gtcaaccttc tggtcaacca catcggagta tggcatggtt gtcttactgc atgaaagtat
     3001 ggtatagctc ataatggcag caagtgtaat ttcagatagc ttcctaatac gcatgatctt
     3061 taaacaattt tagtgtgaaa aaccaagctt gctgagtgca ccatccacct ccggggcaga
     3121 cataaacagg ttccagcaca gcccggttcg gtaattttca atcatgatga tgatcggtcc
     3181 ctgatctata gccaggtacg actttcccca ccacccggca gtggggttaa aagcatcgta
     3241 aaaaccgtag tctccccata gcttgtcgcc taaagtgtag tatagaaatc tagccaccct
     3301 catggattcc tcgggagtat agggcattga cgacagtgca gcggtagggg tgatggttcc
     3361 gttatcattc aatggttcgt gagcgccata gccgttgttg tcgtccgagg cggttaagcc
     3421 ccagcaatca gggctgtaga gcaggtaacc tctagggtct gtaatgcagt actgacggtt
     3481 tatgagcgta tggttaacgt t
//
LOCUS       DAOPHX010000190         3497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_36998_length_3497_cov_5.602557, whole genome shotgun sequence.
ACCESSION   DAOPHX010000190 DAOPHX010000000
VERSION     DAOPHX010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36998_length_3497_cov_5.602557"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            438..1853
                     /locus_tag="PK502_10315"
     CDS             438..1853
                     /locus_tag="PK502_10315"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HPH01250.1"
                     /translation="MVRKIFFIPAGLILLFTSVVVLNSCAGKSTQGEDLSFAELSPAQ
                     RDEFINDLKSRTFRYFWEVVDTNTWQNDDRFPTYNFTSIAATGFALPSYIIGVENNFI
                     SRQQASERVLNTLEWLWSSKQGAEPDATGYKGFYYHFLSYGTGRRYDKVELSTIDTGL
                     LLAGILTCQTYFDGADKTEVRIRELADSIYLRVDWQWAMNGAANMSMGWTPEQGFIQS
                     TWNGYNEAMILLIMAIGSPTHPIDSTAWDSWCSTYKWDSFYGYEHVPFGPLFGHQYSH
                     QFIDFRGIQDSYMRSKGIDYFENSVRATLSNRAYCMENPLKFEGYSANMWGLTACDGP
                     AMAKIRANGRVVQFYDYRARSACSYEIIDDGTIAPTAAGGSIPFAPEECLSALYTMKT
                     MLGSNVYREYGFVDAFNLTVKNGSQGWFDRDYLGIDQGPILIQLENYQSGLIWNLMKK
                     NRYIVSGLKKAGFRNGWLEQS"
     gene            1891..>3497
                     /locus_tag="PK502_10320"
     CDS             1891..>3497
                     /locus_tag="PK502_10320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPH01251.1"
                     /translation="MERKVHFKKFLLLNLLLVLCLPEAFAQKTVSGRVTDAETGETLP
                     GVNILILGKTKGTITNNDGSYTINMEEDENTLVFSFIGYRPDTITVTTSRRVDVQLKV
                     EALFIEDIVVVGYGVQRKSDLTGSVVSVKSEELTRIPTSSPMQALEGKVPGLQVASSS
                     GAPGSGVVVRVRGVGTFNNSNPIYVVDGVILDNIDYLNTADIESMEILKDASATAIYG
                     SRGANGVIIVTTKKSKLGTDKTTFNLSYENSYQKLINRIDLLSGREFATVVNVINPGT
                     YNNIDAVPNTNWQDIIFDDGFSAPIHNIQFSASGATSKMQYYVGIGYYNQTGIINKSN
                     FERLTVKLNNTYQLNKWLNIGNNVTIVPFKQQNTNGNVVFVAYRAWPVLEPYQADGSY
                     TALPGTGNPLADVEYTNSFSNGLKGTGNIFGDATLTKGLVLRSSFGVDLGFNRSKSFS
                     PTYFVSPQQQNSENDLYKCEGTQSSWIWENTLNYNHDFDRHHFDALAGFTMQDVTSEN
                     IGISGINVIRDAEDFWYINSNNINPNSV"
BASE COUNT         1014 a          699 c          793 g          991 t
ORIGIN      
        1 agaacctgtc cgcctaccgg cggaacttgt ccggctcctg acggattcaa catttagcta
       61 ggggcattaa cggcatgggt agcactgact tagtcatacc ctatggttag gttataaatc
      121 gtctaaattt tatcataatg aattaaaaac cacaaaatca atatgttaat attttatagg
      181 tgtaattaaa tggtttcgcc gaaatcgttt tcggctaaaa atatttctat aaatcataat
      241 tctaaagtat cagaaaaaca gtataataaa gtttaaatat taaaaatgac gtactgttaa
      301 gcatgcttaa aaaattccct tttgttataa ccgtagccgt gatgaggcgg aattctggac
      361 aaacgtttta tcgagtgtta actttgagct aaatcaagtt aaaggatata atgatttgta
      421 aattttttgc ttcaactatg gttagaaaaa ttttttttat tccagcaggc ttaatactgt
      481 tatttacctc tgttgttgtt ttaaattcat gcgctggtaa atcaactcaa ggggaagatc
      541 tttcttttgc tgagctaagt cctgctcaaa gggatgaatt tattaatgac ttaaaaagta
      601 ggacatttcg ctacttttgg gaggtggttg acacgaatac ctggcaaaat gacgaccgct
      661 ttccaaccta caattttacc agcattgctg caacgggttt tgcccttccc tcttatataa
      721 taggtgtaga aaacaatttt atttcccgtc agcaagcttc ggaacgagtg ctcaacaccc
      781 tggagtggct ttggagttca aagcagggag ctgagcccga cgcaaccgga tataaaggct
      841 tttactacca cttcctttcg tacgggaccg gcaggagata tgataaggtt gagctttcga
      901 ctattgatac tggcttgctc ctggccggaa tacttacctg ccagacgtac ttcgatgggg
      961 ccgacaaaac cgaagttaga atacgggagc ttgccgattc catttacctg cgcgtcgact
     1021 ggcaatgggc tatgaatggt gcagccaaca tgtccatggg ctggactccc gagcaaggat
     1081 tcatacagag cacctggaac gggtataatg aggccatgat tctactcatc atggctatcg
     1141 gctccccaac ccatcccata gactccaccg catgggacag ctggtgcagc acctacaaat
     1201 gggatagttt ttatgggtat gagcatgtgc cctttgggcc actctttggc catcagtact
     1261 cccatcaatt tatcgacttc aggggaatac aggatagcta catgcgttca aagggaattg
     1321 actacttcga gaactcggta agggctaccc tgagcaacag ggcctactgc atggaaaatc
     1381 cactcaaatt tgaggggtat tcggccaaca tgtgggggct tacggcgtgt gatggccctg
     1441 caatggccaa aatcagggct aatggcaggg tcgtgcagtt ttacgactac cgggcaagaa
     1501 gcgcttgcag ctacgagatt atcgacgatg gaacaattgc tcctacagca gcaggagggt
     1561 caatcccatt tgcccccgag gaatgcctga gtgctcttta taccatgaag actatgctgg
     1621 gtagcaatgt ttaccgggag tacggctttg ttgatgcgtt taacctgact gtgaaaaatg
     1681 gcagccaggg ttggttcgac agagattacc tgggaataga ccagggacca atccttattc
     1741 agctggaaaa ctaccagagc gggctgattt ggaacctgat gaaaaagaac aggtatatag
     1801 tttcaggtct aaagaaggcc ggtttcagaa atggttggct agagcaatcg taaaccaaga
     1861 tatcaaacct aaacttttaa acctatgcaa atggagagaa aagttcattt taagaagttt
     1921 ctgctgttaa acttactact tgtgctatgc ttgcccgaag catttgcaca aaaaacagtg
     1981 agcggaaggg ttaccgatgc cgaaactggc gaaacgctgc ccggagtaaa cattttaatt
     2041 ttgggtaaaa ccaagggtac tattactaat aatgatggtt cttataccat taacatggaa
     2101 gaggacgaaa atactctggt attttccttt attggctata gacctgatac tattaccgta
     2161 acaactagta gaagggttga tgttcagctt aaggtagagg ctttgtttat tgaggatatt
     2221 gtagttgtag gttatggtgt tcagcgtaag agcgatttaa cgggttctgt tgtaagtgtt
     2281 aaatcagaag agttgacacg tattcctacc tcgtcaccca tgcaggcttt ggaaggaaaa
     2341 gtccctgggc ttcaagttgc cagttcctca ggtgcccctg gttcaggggt ggttgtcagg
     2401 gtgcgtggcg taggtacgtt taataactct aacccaatct acgtggtgga tggcgttatt
     2461 ctagacaaca tcgactacct gaatactgct gatatagaat caatggaaat ccttaaggat
     2521 gcttcggcaa cggctattta cggttccagg ggagccaatg gtgttatcat tgttaccaca
     2581 aagaaatcta agcttggcac tgataaaact acttttaacc tctcctatga gaatagctac
     2641 cagaagttga taaatagaat tgatttgtta tctggtaggg aatttgctac tgttgttaat
     2701 gtaataaacc ctggcacgta caacaacatt gatgctgttc cgaacaccaa ctggcaggat
     2761 attatttttg acgacgggtt tagcgctccg attcataaca tccagttttc ggcttctggc
     2821 gcaacatcca agatgcaata ctatgtgggc attggctatt acaatcagac tgggataatc
     2881 aataagtcga attttgaaag actaactgtt aaacttaaca atacatacca gctaaacaag
     2941 tggctaaata ttgggaataa tgtaacgatt gttcccttca aacagcagaa caccaatggt
     3001 aatgtggtat ttgttgctta ccgggcatgg cctgttttag aaccatacca ggcagatggt
     3061 tcctatactg cacttcccgg cacaggaaat ccactggccg acgttgaata caccaacagc
     3121 ttcagcaatg ggttgaaggg aacaggcaat atttttggag atgctacttt aactaaagga
     3181 ctagtacttc gctctagctt tggtgttgat ttgggcttta accgaagcaa atccttttct
     3241 cccacttatt ttgtatctcc tcaacaacag aattcagaaa acgatctata caagtgtgaa
     3301 gggactcaga gctcttggat ttgggagaac accttgaatt acaaccatga tttcgatagg
     3361 catcacttcg atgcgttggc tggttttaca atgcaggatg ttacttccga aaatattgga
     3421 attagcggta ttaatgttat tagggatgca gaagattttt ggtacatcaa ctccaacaat
     3481 atcaacccaa attcagt
//
LOCUS       DAOPHX010000191         3440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_37875_length_3440_cov_5.299261, whole genome shotgun sequence.
ACCESSION   DAOPHX010000191 DAOPHX010000000
VERSION     DAOPHX010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37875_length_3440_cov_5.299261"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PK502_10325"
     CDS             <1..257
                     /locus_tag="PK502_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01252.1"
                     /translation="FGSSAGVAITNKFQEAKNVTLWLRKGWHVTLAYIVGFFTLYITM
                     AWEPASNKQHQEPAIECKAPDCPVRAKAEFERTLHPDEVK"
     gene            complement(552..1742)
                     /gene="ygeW"
                     /locus_tag="PK502_10330"
     CDS             complement(552..1742)
                     /gene="ygeW"
                     /locus_tag="PK502_10330"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF005538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HPH01253.1"
                     /translation="MDRVEIIERLKTNLHGRDFLLTWEKSEDELRMILGVADILKEMR
                     NRNISTRVFDSGLAISNFRDKSTRTRFSFASASNMLGLAVQDLDEEKSQISHGETVRE
                     TANMISFLSDFIGIRDDMYLGEGNKYMRQVGTALDDGFANGVLPVRPGIVNLQCDMDH
                     PTQVMADLLHLTHEFGSLEALRGKKLVMSWAYSPSYGKPLSVPQGVIALMTRFGLNVE
                     LAYPEGYDLIPEIVDVARNNAAKSGGSFTIKHSMAEAMRGADIVYPKSWAPFHIMEQR
                     TQLLKSGDKKLLNELEQTCLANNARHKDWEYNEEMRRLTSKSDALYLHCLPADISGES
                     CAQGEVSAETFERYRIKTYLEAGYKPYIIAAMMLANRFEDTAGLMKKLLDRKQERVTS
                     VLKL"
     gene            complement(1894..3081)
                     /locus_tag="PK502_10335"
     CDS             complement(1894..3081)
                     /locus_tag="PK502_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HPH01254.1"
                     /translation="MSEISLKVKELSEKYRDYTASNLSRLVRIKSLSTQEHDVQLELK
                     RQMEEAGFDEVRIDGLGNVVGRIGNGSRILAIDGHMDTVDIGYRNNWDFDPLGGEIRD
                     GFVHGRGSVDQKGGPASFVTSGRILKELGFDRDLTIYFVGSVMEEDCDGLCWKYIIEE
                     DGIKPDFVISTEPTNLNIYRGHRGRMEMHVQFYGVSAHGSAPERGKNAIYMASRAALE
                     IEKLNEHLKVDEFLGKGSVTISEIVSGSPSLCAVADYARIHLDRRLTWGETMETAVGE
                     VEEIVKNMNAKVEVLNYSEKAYTGLEYGMVKYYPTWKMSEDHLIVQTGVKAFSQLYGK
                     NPKVDKWTFSTNGIMTCGVYGIPTIGFGPGNEVLAHAPNEKVPVNDLVVASAFYTLFA
                     YTI"
BASE COUNT          913 a          853 c          725 g          949 t
ORIGIN      
        1 ggtttggttc gtcggctggc gtggctataa ccaataagtt ccaggaggct aaaaatgtaa
       61 cgctatggtt acgaaaaggc tggcacgtta ccctggccta tattgtcggt ttctttaccc
      121 tatacatcac catggcatgg gaacctgcca gcaacaagca gcaccaggaa cctgcaattg
      181 agtgtaaggc acccgactgt ccggttaggg caaaagctga atttgagaga acattgcacc
      241 ccgatgaggt taaatgatct gtaactccgg aatagagaaa acactttgtg ctttagctat
      301 taaattttat aagtgaagaa ggtgctacca gctagaaatt ttagctaaat taaaaaacag
      361 aggaattgct gaaataaaag tcaacccttt tggttgattt tcctaaaagc gaaaaaacct
      421 gccccccaca tagatggttc cggcaggttt tttcgctatt tccttagtaa gctatatggg
      481 cagaaaaaat tatggtttgc ccaacccggc tagaattgtt cttaccctgg cgcaaagctc
      541 caactacctt cttacagctt taaaacactg gtaacccgtt cctgcttacg gtctagtaat
      601 tttttcatta aacctgctgt atcctcaaag cggttagcaa gcatcatggc tgcaattatg
      661 tagggcttat atcccgcctc aaggtaggtc tttatgcggt agcgctcaaa agtttcagca
      721 cttacctcac cttgtgcaca cgattcaccg gaaatgtcgg cgggcaggca gtggaggtaa
      781 agggcgtcgg acttactggt caacctacgc atctcctcgt tatactccca gtccttatgc
      841 cgggcattgt tagccaggca ggtttgctcc agctcgttca gtaacttctt atcgccactt
      901 tttagcaatt gggttcgctg ctccatgatg tgaaatggag cccagctttt tggataaact
      961 atatcagcgc cgcgcatggc ttcggccatg gaatgtttaa tggtaaacga tccgccgctt
     1021 ttggcagcat tattcctagc tacatcaaca atctcgggta ttaagtcgta accttccggg
     1081 tatgcgagct ccacattcaa gccgaaacgg gtcataaggg caataacccc ctgggggacc
     1141 gatagaggtt ttccgtagct aggggagtaa gcccaactca tcaccagctt tttacccctc
     1201 aaggcttcca gggagccgaa ttcatgagta aggtgaagca aatcggccat aacctgggtt
     1261 gggtggtcca tatcgcattg caggttgaca atacccggcc tcaccggtag cacgccgttg
     1321 gcaaacccat cgtcgagtgc tgtgccaacc tgccgcatgt acttgttacc ttcgccaagg
     1381 tacatatcgt cgcgaatgcc tataaaatcg gatagaaacg aaatcatgtt ggcagtttca
     1441 cgaacggttt ccccatgcga aatctgcgat ttctcctcgt caagatcctg aacagccagc
     1501 cccagcatgt tggaggccga tgcaaaagaa aatcgggtac gggttgactt gtccctaaaa
     1561 ttggaaattg ccaggcccga gtcgaatacc cttgtagata tattacggtt tctcatctcc
     1621 ttaaggatat cggctacccc aagtatcatc cttaactcgt cttcactttt ctcccacgtt
     1681 aggaggaaat ccctgccatg caggttggtt tttaaccttt cgatgatttc aactctatcc
     1741 atcaacataa attgtttaaa taaacagaca actttttaac tcgattagca atagcaatag
     1801 gcagcttcca ttaaaattcc tctaaacaaa aataacagca gcatcactgc cctaaaatgg
     1861 taaagaaaaa gtaaacctaa aaatttcaac cagctagatg gtgtaggcaa atagggtgta
     1921 aaatgctgag gcaaccacca aatcgttaac aggcaccttt tcgtttggag catgagccag
     1981 cacctcgtta cccgggccaa atcctatggt tggaattccg taaacgccac aggtcataat
     2041 accgttggtt gaaaatgtcc acttgtcaac ctttgggttt ttgccataaa gctggctgaa
     2101 tgctttaacc ccggtttgca caattaaatg atcttcagac attttccaag ttggatagta
     2161 tttaaccatg ccgtactcca ggccggtgta ggccttctcg gagtagttca gcacctcaac
     2221 cttggcattc atgtttttaa caatctcctc cacctcgcca acagccgttt ccatggtttc
     2281 accccaggtc agcctgcggt cgagatgaat tcgggcataa tcggctaccg cacatagcga
     2341 cggactcccc gatacgattt cagaaattgt aacgcttccc ttgcccaaaa attcgtcaac
     2401 cttcaggtgt tcgttgagct tctcaatctc cagagcagcc cttgaggcca tgtagatggc
     2461 atttttgcca cgctcaggag ccgagccatg ggctgacacc ccgtaaaatt ggacgtgcat
     2521 ctccattctt cccctatgcc ctctgtagat atttaggtta gttggttcgg tgctgattac
     2581 aaaatcgggt ttaatcccat cctcctcgat gatgtacttc cagcatagcc cgtcgcaatc
     2641 ctcctccatt acgctgccga cgaagtaaat ggtgagatcg cggtcgaagc ccagttcttt
     2701 cagtattcgt cctgaagtta cgaatgaggc tggaccacct ttctggtcaa cagagcccct
     2761 tccgtgcaca aaaccatcgc gaatttcacc tcctaacggg tcgaagtccc agttgttgcg
     2821 gtaaccaatg tcaacggtat ccatgtggcc atctattgcc aatatcctac taccgttgcc
     2881 tattcggcca actacattcc ctaacccatc aatccttacc tcatcgaagc cggcttcctc
     2941 catttgtctc tttagctcca gctgaacatc atgctcctgg gtgcttaggg attttatcct
     3001 gaccaaacgc gacaagttac tagccgtata gtcgcggtat ttctcggaaa gttccttaac
     3061 cttcaagcta atctcactca tgatgattat cctattttgg ttcaaaatta ccggatatat
     3121 caatagccac atcaagtttt agttgtaaat tatacatttt attcaaattt agtatttttc
     3181 tcatagtatt aacttacatt ataggtattt tttctatata taaaggctaa ctaaaggatg
     3241 gttaatatag ctgtaccatg aaaatggtta aatattaaag gaattcaggg cccgtcaaac
     3301 tctgacggag cttgtgtaac tcttggcgga gaaaagttaa atgttacgcg gtcgggaacc
     3361 gagccgtttt ttggcgagtt cgccgaaggc aaaccgctac ttaaatacga cgaagttacg
     3421 ctttgctaaa cttcgccgag
//
LOCUS       DAOPHX010000192         3432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_38016_length_3432_cov_5.644359, whole genome shotgun sequence.
ACCESSION   DAOPHX010000192 DAOPHX010000000
VERSION     DAOPHX010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38016_length_3432_cov_5.644359"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="PK502_10340"
     CDS             complement(<1..1631)
                     /locus_tag="PK502_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPH01255.1"
                     /translation="MSLSSLSINRPVLSTVFSLIILLLGAIGITFLGVREFPSVDPPI
                     ISVSTSYPGANSDVIETQITEPLEQSINGIPGIRTLTSRSSQGSSRITVEFELSVDME
                     TAANDVRDKVSQAQRLLPRDCDPPTVSKADADARPIMNIAINSKKRSLLELSEIADLT
                     LKEQLQTISGVSSVGIWGEKRYAMRIWLDPAKLAGYQMTPLDVRNALNRENVELPAGS
                     IEGANTELTIRVLGLMSTPEEFNNLILKRSGEQLIRVRDIGRAELAPEDLRSIMKMNG
                     VPMVSVVVIPQPGANQIEIVDEVYKRIDFIKKDLPDDVNIVVGFDNTQYIRSSIKEVQ
                     TTIYIAFFLVVVIIFAFLRDWRTTLIPILVIPVSLVGVFFIMYLAGFSINILTLLAIV
                     LSIGLVVDDAIIMMENIYVKIEQGMTPLEAGVKGSNEIFFAIIATTITLISVFFPIVF
                     LEGMTGRLFREFSIVIAGAVAISAFVALTFTPMLSTKLLKTRHTRSKAYSYTEKFFTS
                     LNSGYRNSLSSFLKHRYLPVVILVVAAGLIVLLWK"
     gene            complement(1637..2758)
                     /locus_tag="PK502_10345"
     CDS             complement(1637..2758)
                     /locus_tag="PK502_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPH01256.1"
                     /translation="MTAFSKRLIRISTTVAIALLLVGIILYPKVKDASNSDSKDKVPG
                     RMAMRGGGGQPLFANGFIIVPTSMSELISTTGSLLPDEEVDLSFETSGKVVAIYFEEG
                     TKVKKGDLLAKINDAPLQAQLQKLQAQRKLAEEKLFRQKQLLERDAISRESYDQVSTE
                     LQAIEADIMLIKARIAETELRAPFDGIVGLRLISEGAYTSTQTPIAHLVKVSPLKIEF
                     SIPERYAGEVTPGYDISFVIDGIESKFTGKVYAVAPKVDMATRAIVVRAMYPNPNEEL
                     KPGRYASITLQLSKIENAVSIPTEALIPEMEGEKVFIYRNGKAEQVRVTTGLRTESRI
                     QVRTGLNFGDTLLTSAILQLRQGIPVQLDTIITNAINLQ"
BASE COUNT          959 a          804 c          773 g          896 t
ORIGIN      
        1 cttttccaaa gtagaactat caggccagca gctaccacaa gaataacaac tggcaggtac
       61 ctatgcttca gaaagctgct tagcgaattc ctgtacccac tattcaacga ggtaaaaaac
      121 ttttcggtgt agctatacgc cttgctcctg gtatgccggg ttttaaggag cttggtggaa
      181 agcatggggg tgaaagtcag ggcaacaaaa gccgaaatgg caactgctcc tgcaattaca
      241 atactaaact cccgaaacag cctccccgtc ataccctcaa gaaaaacaat gggaaagaat
      301 acggaaatca aggtaatggt tgtggcaatg atggcaaaaa atatttcgtt cgatccctta
      361 acacccgcct ccaacggggt cataccctgc tcaatcttaa cgtagatatt ctccatcatg
      421 atgatggcat cgtcaaccac caaaccaatg gagagcacta ttgccagcag ggttaaaatg
      481 ttgatgctga accctgcaag gtacattatg aagaatacgc caaccagcga aacggggatt
      541 accaggatgg ggataagggt ggttcgccaa tcgcgaagga aggcaaaaat gataaccacc
      601 acgagaaaaa atgcaatgta aatggtggtt tgcacctcct taattgacga ccttatatac
      661 tgggtgttgt cgaaacccac cacaatattt acatcatcgg gcaggtcttt tttaataaaa
      721 tcgatacgct tgtaaacctc atccacaatt tcaatttggt ttgccccggg ctgtggaata
      781 acaactaccg aaaccatagg cacaccgttc attttcataa tgcttcgtag gtcttcgggg
      841 gccagctcag cccttccgat atcacgaacc ctaatcagct gctcgccaga acgtttaaga
      901 attaaattgt taaattcctc gggtgttgac ataaggccca gcacccgaat ggtaagttcg
      961 gtgtttgccc cttcaatact gcccgcagga agctccacat tttcacgatt cagcgcattt
     1021 ctaacgtcaa gcggagtcat ttggtacccg gccagcttcg cagggtcgag ccatatacgc
     1081 atggcgtacc ttttctcgcc ccaaatgcca accgagctaa cgcccgaaat ggtttgcagc
     1141 tgctctttca gagtaagatc ggcaatttcg gaaagctcga gcagcgagcg cttcttgctg
     1201 ttaatggcaa tattcattat gggacgcgcg tcagcatcgg ccttcgaaac agttggcggg
     1261 tcgcaatcgc gtggcaaaag acgctgtgcc tgcgaaacct tatcacgcac gtcatttgca
     1321 gcagtctcca tgtcaactga aagttcaaac tccacggtaa tcctgctgct tccctgacta
     1381 ctccggctgg taagggtgcg aattccggga atgccgttga ttgactgctc caggggttcg
     1441 gtgatctggg tttctataac atcggagttg gctccggggt aggatgtgct caccgagatg
     1501 attggcgggt caacgctagg aaattcccta acacccagaa aggttatacc tattgcgcca
     1561 agtagcagga tgatgaggga aaatacggtg gaaagtaccg ggcgatttat acttaatgat
     1621 gaaagactca tgtgctttac tggagattta ttgcattagt aataattgta tcaagctgaa
     1681 caggaatgcc ctgtcgcagc tgaagaatgg cggaggtaag cagagtgtcg ccaaagttga
     1741 ggcctgtgcg cacttgaatg cgcgactcgg tgcgtagccc ggtggtaacc ctaacctgtt
     1801 cggcttttcc attcctgtag atgaatacct tttcaccctc catttcgggg attagcgcct
     1861 cggtggggat tgaaacagcg ttttcaattt ttgaaagctg cagggtgatg ctggcataac
     1921 gaccaggctt cagctcttcg ttgggatttg ggtacatagc cctaaccacg atggcccgtg
     1981 tagccatgtc aaccttgggt gctaccgcgt aaacctttcc ggtaaactta ctttcaattc
     2041 cgtcaataac aaagctaatg tcatagcccg gggttacctc cccggcgtac cgctcgggaa
     2101 tggaaaattc aattttaagg gggctaacct taaccagatg ggctattggg gtttgggttg
     2161 aagtgtaggc accctcgcta atcaagcgca acccgacaat tccatcaaat ggtgcccgta
     2221 gctccgtttc ggcaatacgc gctttaatga gcatgatatc agcctcaatg gcctgtagct
     2281 ccgtagatac ctggtcgtag ctctcccgac tgatagcatc gcgctcaagt agctgcttct
     2341 ggcggaacaa cttttcctcg gccagcttgc gttgagcctg aagcttctga agctgagcct
     2401 gcagcggggc atcgtttatc ttggccaaca aatcgccctt tttcactttt gttccctcct
     2461 caaagtaaat ggctacaacc ttcccggatg tttcaaatga aaggtcaacc tcctcatccg
     2521 ggagtaagct gccggtggta gagatcaact cgctcatgga tgttggaaca ataataaaac
     2581 cgttggcaaa aagcggctgg cctcccccac cccgcatggc cattcgtcct ggtactttat
     2641 ctttagaatc actatttgaa gcatctttta ccttgggata cagaataatc ccaactaaaa
     2701 gtagagcaat agcaaccgta gtagatatcc tgataagcct tttagaaaat gctgtcattt
     2761 gatgctgatt ttattttaag tttactagtt aacacctttg cctagtatga cagcaaaagt
     2821 taaaaaaggt tgaaaaacag aaattcattt ttcaacaaaa tttaacaaag agaatcaaaa
     2881 tataagggat aaaaaaatta acctttcaca tggtcagggt tctcaattac atggaacaat
     2941 aaaaatcaag aagttacctt gctaataaca ggaagaaatc gagaaatcga gtctaatgct
     3001 catcagcaca cgtgctaaca tttatccttt tccctttaat tccttacgct tcgacagggt
     3061 tatgccaggc attcaacctt taggattttt ttattaattt cggggagctt gttaggcaat
     3121 caccgataca ggtaaaagta agctttcaat gatatgaaac atacttataa tgtagcttgc
     3181 tcaaacacgc gttagcaaaa tatgggtgtt ccatccgccc aaccccgatg ccttaaaggt
     3241 atgggtcaag gtatttgtaa tcagaaattt aaaaattttg tacggatgaa accacaaagt
     3301 gtaatcagca aattttttca ctacttcaac ctcgagtccg agctcgacag ctttgatcgc
     3361 attcatgaaa gcatcgtaaa ggacgtggtg ttcaggggaa ccaacctgtg gattttaatg
     3421 tttgctatca tt
//
LOCUS       DAOPHX010000193         3362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_39122_length_3362_cov_5.852737, whole genome shotgun sequence.
ACCESSION   DAOPHX010000193 DAOPHX010000000
VERSION     DAOPHX010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39122_length_3362_cov_5.852737"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(602..1837)
                     /locus_tag="PK502_10350"
     CDS             complement(602..1837)
                     /locus_tag="PK502_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPH01257.1"
                     /translation="MHLPIYIARRYLFAKKSHNVINIISIISLVGVATGVMALVVVLS
                     VFNGFDTLIERLYSNIDADIKILPREGKTFSITGEKFDKLAKMNGVAVFAEIFEENAL
                     FSYRGKQHIGIIKGVSRNYSELTGLDSMMKDGEFKLWMGSQPMAVVGQGVAYYLNASL
                     AQFDPLFVYIPKRGRASLTMESAFGKKAIMPSGIFSVEQDFDTQYVIAPVEFVRNLLG
                     YDSTMVTAIEVKVAGGVDAESIRRSMEEVLGSDFRVLNRYQQNESLYRTMKSEKFAIG
                     LILTLILVIASFNIVGSLSMLIIDKRKDVETLRSLGADNGLIKKIFMVEGMLISIGGT
                     IIGTLLGLLICWLQVHFKLITLEGRGNFIIDAYPVDIQLPDIGAIILVVLLIGYLAAR
                     FPVRMITRRILNEDIRSNE"
     gene            complement(1926..2699)
                     /locus_tag="PK502_10355"
     CDS             complement(1926..2699)
                     /locus_tag="PK502_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPH01258.1"
                     /translation="MMRQVISLFTRFVPRHRLQVFAAFVLRVVGIFYRGNRYEDPISG
                     KTYRKLLPYGRTVVRPNALAPHSMSLERHRLIWLYLRERTDFFTAPHSVLHIAPEYCF
                     LKPFRKLGNIEYVTADLISPWADVKLDVQAMPFPSSQFDVVICNHVLEHVQSDIKAMG
                     EIYRVLKPGGFAILQVPIDISREKTLEDPSINTPELREQYYLQRDHQRLYGRDYADRL
                     RSVGFRVDENHFVDELPLNLVERYALPVGEVLFICNKPD"
     gene            complement(2713..3051)
                     /gene="rbfA"
                     /locus_tag="PK502_10360"
     CDS             complement(2713..3051)
                     /gene="rbfA"
                     /locus_tag="PK502_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPH01259.1"
                     /translation="MEGPRLQKVDRLVQKELGDIFQREGQARLRGKIITVTRVRVSPD
                     LSLAKVYISIFPSKEGAQTLELLNEHKKAIRGELGRRVRHQLRIVPELAFFIDDSLDY
                     IEHIDDLLKS"
BASE COUNT          885 a          850 c          793 g          834 t
ORIGIN      
        1 cttaagggca ttgatgaagt attccttatc gggaaggagc ttcagagccg gttgttccat
       61 aaccttgcaa gctctgcatc gaacctactc cccttcggcg aagtttgtag cttcgtcgtt
      121 tataaatcgc ggtttcccga ccgccgttaa aaaaattact cttccccatt ctaaagtaaa
      181 gctgcacaca tactacaacc cttaagccaa gctacaagta gaatcttgca cggccagaca
      241 cctctactca ggtacggcta agttgcagac atagccgagc gagggcaccg tgaaaagctg
      301 ctcatagccg ctgttctggt aaacaacttt accatttgat tttgaaaaaa tcaaatttta
      361 cctgattttt ttgattaacc tggtaagctg ccactcacag aattaccctt tttacacatg
      421 gttttggcta tccctggggt gcaattcctg gctaaatgct gcgacccata ctaagcccta
      481 gggtcgtcac cctggcattt ggttttgtgg acatgggcgg agaggctgaa gccgggaagg
      541 gaaatgtggt cagagggctg tgcctgacaa gcatgtaaac cgcccaaaaa atgcccaaag
      601 cctactcgtt actacggata tcctcattca gtatgcggcg ggtgatcatc cgcacgggga
      661 atcgggctgc caggtagcca atcaggagaa ccaccaggat aattgcccct atgtccggca
      721 gctggatatc aaccgggtag gcatcaatga tgaagttgcc tcgaccttca agggtaatca
      781 acttgaaatg cacctgaagc cagcaaatga gcagtcccaa aagtgtcccg ataattgttc
      841 cgccaatgga aatgagcatg ccctcaacca tgaatatttt tttaataagg ccgttgtctg
      901 cacccaggct gcggagggtt tccacatcct tgcgcttatc aatgataagc atggagagcg
      961 accccacgat gttgaaggag gctataacca gtatgagagt taaaatcagc cctatggcaa
     1021 acttctccga cttcatggtc cggtagagcg actcgttctg ctggtaccta ttcagcaccc
     1081 tgaaatcgct gcccagtacc tcctccatgc tcctcctgat gctctcggca tccaccccgc
     1141 cggccacttt gacttcaata gcagtaacca tggtggagtc gtaacccagc aggtttcgca
     1201 caaactcaac cggcgcaatg acgtactgcg tatcaaaatc ctgctcaacg gagaaaattc
     1261 ccgacggcat gatggccttc ttcccaaaag ccgattccat agtaagggat gccctccctc
     1321 ttttgggaat gtaaacaaaa agggggtcga attgcgccag gctggcattg aggtagtagg
     1381 ccacaccctg ccccaccaca gccatgggct ggctacccat ccatagcttg aactcgccgt
     1441 ctttcatcat ggagtcgagt ccggtaagct cgctgtagtt ccggcttacg cctttaataa
     1501 tgcctatgtg ctgcttgcca cggtagctga atagggcatt ttcttcaaaa atctcagcaa
     1561 aaacggctac tccattcatt tttgccagct tgtcgaattt ttccccggta atgctgaagg
     1621 ttttaccctc acggggaaga atcttaatat cggcatcaat attcgagtaa agccgctcga
     1681 taagggtgtc gaacccgttg aacaccgaaa gcacaaccac cagcgccatt accccagttg
     1741 ccacccctac cagcgaaatg atggagatga tgttgatgac attatgcgat ttcttcgcaa
     1801 agaggtaacg acgggcaatg taaatgggca ggtgcatatc cttagcaaat gcttttggct
     1861 aaaggcgtaa tatatacctt gtaatttgat tgatatggag aaaatctctt cacattaata
     1921 attatctagt ccggcttatt gcagatgaaa agcacctcgc ccacaggcaa ggcatagcgt
     1981 tcaacgaggt ttaacggcaa ctcatccaca aagtgatttt cgtcaaccct aaagcccaca
     2041 cttcgcaggc gatccgcata atccctaccg taaagtcttt gatggtcgcg ctgtaggtag
     2101 tactgctcgc gcagctccgg ggtgttgatg gaggggtctt caagggtttt ctccctgctg
     2161 atatcaattg gcacctgaag aatggcaaaa cctccgggtt taagcaccct gtaaatctcc
     2221 cccatggcct taatgtcgga ctgcacatgc tccagcacat ggttgcagat taccacgtcg
     2281 aactgggagc tggggaatgg cattgcctgc acgtcgagct taacatctgc ccagggggag
     2341 atcagatcgg cggtcacgta ttcaatgtta cctagttttc tgaagggttt aaggaagcag
     2401 tactccggtg caatgtgaag tacgctatgc ggagccgtga aaaaatcggt gcgctcacgc
     2461 aggtatagcc aaataagccg gtgacgctcg agcgacatgg agtggggcgc cagggcattc
     2521 ggcctgacaa cagtgcgacc gtagggcagc agcttgcggt aggttttccc agagatggga
     2581 tcctcatagc ggttgccgcg gtagaaaata cccaccaccc taagcacgaa tgcagcaaaa
     2641 acctgaaggc gatgtcgggg aacaaaccgg gtaaacagcg aaatgacttg cctcatcata
     2701 ccggtggccc tattatgatt ttaggagatc atcaatatgc tcgatgtagt cgagcgagtc
     2761 gtctataaaa aatgccagct ctggcacaat gcgcagctga tggcgcacac gcctaccaag
     2821 ttcaccacgt atggcctttt tatgctcatt cagcagctca agagtttgag ccccctcctt
     2881 ggaggggaaa atgcttatgt acaccttggc caacgacagg tcggggctaa ccctaacacg
     2941 ggttacagta attattttgc cccgtagcct ggcctgcccc tcccgctgga aaatatcgcc
     3001 cagctccttt tgaaccagcc ggtcaacctt ttgaagcctt ggtccttcca tagctctctt
     3061 tttggcaaag atacatgatg aagttgatat cgtaaaactt caagctattt gtaagcggtg
     3121 agccaaacaa aatcggatct attgcttacc tatgaggtaa taacgcttaa ttgttccaaa
     3181 attgacctgg ggatgagatg tattatgttt gagcaagttt tcccaagggt taaatgggtt
     3241 aggtacctaa cttggtacaa taaaagttcc caacttaaaa caacaatatt ttacccgtcg
     3301 ttcttgtggt ataaatttct aatcaaaatt tgtaggtcaa gcgaaacgct gtagttacgg
     3361 gc
//
LOCUS       DAOPHX010000194         3221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_41484_length_3221_cov_5.135818, whole genome shotgun sequence.
ACCESSION   DAOPHX010000194 DAOPHX010000000
VERSION     DAOPHX010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41484_length_3221_cov_5.135818"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PK502_10365"
     CDS             <1..193
                     /locus_tag="PK502_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01260.1"
                     /translation="SALIFFGSFLYQDKKEHIAGSLPQFRHGDEKNPNSSLEQLRFAS
                     PTHPLQALHFKTIPPLQGL"
     gene            complement(328..>3221)
                     /locus_tag="PK502_10370"
     CDS             complement(328..>3221)
                     /locus_tag="PK502_10370"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPH01261.1"
                     /translation="NAWVAIHILNHKLGLGSSPQTIFNMSVGYNLEGIKKPNVQWFLD
                     RMTSCETELKTKINLVRPIYPAIDSILIPPQLSNSITLSTMHGCPANEIEGIARYLLT
                     ERKLHTLVKLNPTLLGRQELRRILNDELKFKTSVPDAAFEHDLKYPDAVQIIRSLQGD
                     AERMGLQFGLKLTNTLESVNNRGTFKSEVEMMYMSGRALHPISINLASKLQNEFDGQL
                     LISFSAGVDAFNVVDTLKCGFRTVTVSSDLLKPGGYMRLNQYIEAISAELLKNKLDSV
                     GGLAHLKNGAAPTDYLSSYAGRVIENKRYQRSYLHEPTIKTTRELDYFDCISAPCRDT
                     CAANQDIPDYLHHAANSQFDKALEVILQTNPFPSVTGMVCDHLCQNKCTRINYDNPLL
                     IREVKRFVAENASIKLSQKPLNGKQVAIVGAGPSGLTCAYYLAQAGFKVDVYESESKA
                     GGMVRYAIPGFRLTSEALERDIARILQLGVTIAYNHRITSEEFSKLKSTYDFLYISAG
                     AQKATPLNIDGIEAAGVYDSLAFLKDVKAGNIKGVGKNVVVIGGGNTAMDAARTAHRL
                     VGTNGKVTVVYRRTIDEMPADEGEIKAVMAEGAEIVELSAPERLIIEKGNVCGLVCSR
                     MKLEGTDAKGRPKPVRVNNSEFTIPCDTVIPAIGQEVDIDFVPKELLRAHTTTYKTLL
                     HNVYIGGDALRGAATAIKAIGDGRKVAEQIINQAGIQRSIKTPTGRSHSKEELIMKRA
                     KRSYAPELPELSPSESRTFSLVSQTLDEQSIVSESGRCLQCDEICNICTTVCPNLANF
                     SYDISPVKYNMQRVLVGEDGQLKFEPDGVFEVKQQYQILNIANFCNECGNCATFCPTS
                     SAPYLNKPKLHLTTKSFNSSDEGYMLSKLEDRNVLIYKNEGGIKTLSETAGELIYETD
                     HVAARFDHDTFELKEVNVKTPCVRQIQFQHAAEMSIILKGAKARIKDKG"
BASE COUNT          763 a          773 c          732 g          953 t
ORIGIN      
        1 ttcagccttg attttctttg gttctttctt gtatcaagac aagaaagaac acatagctgg
       61 ctctttgcca caattccgcc atggtgatga aaagaatcca aacagctctt tggagcagct
      121 acgatttgca tcgccaacac accctctcca ggctcttcat ttcaaaacta ttccacccct
      181 tcagggcttg taaaaaatcg ttcgctccgt acaaggggct gcgccccttg ctacttatta
      241 cgccccttcg gggcttaaat ccgtcaggtt accgacaagt tccgccagga tgcggacaag
      301 ttattgaatt aacttaacct aaacctctta tcctttatcc tttatccttg cttttgctcc
      361 cttcagaatg atactcattt cggcagcatg ctggaattgg atttgtctaa cgcacggagt
      421 ttttacatta acctccttaa gttcaaatgt atcatggtca aatctggcag ctacatgatc
      481 ggtttcatat attagttcac cagctgtttc ggaaagggtt ttaatccctc cttcattttt
      541 gtatatcaac acatttctat cttccagttt tgagagcatg tagccctcat cgctgctatt
      601 gaacgacttg gtagtgaggt gaagttttgg cttgttaagg tagggtgcgc tgctggtagg
      661 acaaaaggtt gcacagttgc cacactcgtt acagaagttg gcaatgttta aaatttgata
      721 ctgctgctta acctcaaaca ccccatcggg ttcaaacttt agctgtccat cttcgccaac
      781 caacaccctt tgcatgttgt actttacggg cgaaatatcg taggagaaat tggccaggtt
      841 ggggcacacc gttgtgcaga tgttgcaaat ctcatcgcac tgaaggcacc tgcctgattc
      901 tgaaacaatt gattgttcgt caagagtttg gctaaccagg ctgaatgtac gactctcgga
      961 tggtgatagt tctggaagct caggggcata cgaccgtttt gcccttttca taatgagctc
     1021 ctctttagaa tggctcctgc cggttggagt ttttatgctt ctttgaatac cggcctggtt
     1081 gattatttgc tctgctactt tccgtccatc accaatggcc ttgattgccg tagcagctcc
     1141 gcgtagggca tcaccgccaa tgtaaacatt atgtagcagc gttttatagg tagttgtatg
     1201 ggcccgtagg agttctttgg ggacaaaatc aatatctacc tcctggccta tagccggaat
     1261 aaccgtatcg cagggtatgg taaattcgga gttattcacc ctaaccggct tggggcgacc
     1321 ctttgcatcg gttccctcaa gtttcattcg gctacatacc aacccgcaaa cgttaccctt
     1381 ttctataata agcctttcgg gagctgacag ctcaacaatt tcggctccct cagccatcac
     1441 cgctttaatc tcaccttcat cagccggcat ttcgtcaatg gttcgtctgt aaacgacggt
     1501 taccttgccg ttggttccta ccagcctatg agcagttcga gcagcatcca ttgcggtgtt
     1561 tccaccgccg attacaacca cattttttcc cactcccttg atgttccctg ctttaacatc
     1621 cttcagaaaa gctaacgagt cgtaaactcc ggctgcctct attccatcaa tattcaaagg
     1681 ggttgctttt tgtgctcctg cagaaatgta aaggaaatcg tacgtgcttt ttagcttgga
     1741 aaattcctcg ctggtaatgc gatggttata tgcaatagta acacccagct gcaggatgcg
     1801 cgcaatgtcg cgctcaagag cctcgctggt taatcggaag ccaggaatgg cgtatcgaac
     1861 cattccgccg gctttagatt ccgattcgta aacatcaact ttaaaaccag cttgcgccag
     1921 gtagtaggcg cacgttagtc ctgatgggcc cgctccaaca atggctacct gctttccgtt
     1981 caaaggcttt tggcttagct taattgaggc attctcagca acaaatcgtt ttacctccct
     2041 gattagcaaa ggattgtcgt agttaatccg tgtgcacttg ttctggcaca aatggtcgca
     2101 aaccattcct gttacggatg ggaaagggtt tgtttgaagg attacctcta gggccttgtc
     2161 aaattggctg ttggcagcgt ggtgcaggta atcgggaata tcctgattgg cagcgcaagt
     2221 atcacggcat ggagccgata tgcaatcgaa gtaatcaagc tccctggtag ttttaattgt
     2281 cggctcatgt aggtagctac gctggtagcg tttattttca ataaccctgc cggcgtagct
     2341 gctcaggtag tcggtaggcg cggctccatt tttcaggtgg gcaagcccgc caacactatc
     2401 aagcttgttt ttcaatagtt ctgctgaaat agcctcaatg tactggttta acctcatgta
     2461 gccacccggt ttaagaaggt cggaacttac tgtaacagtc ctgaagccgc acttcagggt
     2521 atcaactacg ttgaaggcgt caacgcctgc tgagaatgag ataagtagct gcccgtcaaa
     2581 ttcgttttgc agcttgctgg ccaggtttat tgagatgggg tgtagcgcac gtccgctcat
     2641 gtacatcatt tcaacttccg acttgaaagt gccgcgattg ttaaccgatt ccagcgtgtt
     2701 ggttagcttt agaccgaatt gcaaccccat tctttctgca tctccctgta gcgaacgaat
     2761 aatttgaact gcatcggggt acttaaggtc atgctcaaag gcagcatcag gtacagaagt
     2821 cttgaatttc agctcatcat tcagtatcct tctaagttcc tggcggccga gtagtgttgg
     2881 attcaacttg acaagagtgt gcaactttcg ttcagtgagc agatagcggg caataccttc
     2941 tatttcattg gcagggcatc cgtgcatggt tgatagggta atgctgttcg aaagctgggg
     3001 aggaattaaa atgctgtcaa ttgccgggta gatgggccta accaaattaa tttttgtttt
     3061 aagttctgtt tcgcagctgg tcatcctgtc gagaaaccac tgaacgtttg gcttttttat
     3121 gccctccagg ttgtagccaa cgctcatgtt aaaaatagtt tgcggacttg agccaagccc
     3181 gagcttatgg ttaaggatat gaatggctac ccaggcattg a
//
LOCUS       DAOPHX010000195         3076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_44147_length_3076_cov_6.417080, whole genome shotgun sequence.
ACCESSION   DAOPHX010000195 DAOPHX010000000
VERSION     DAOPHX010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44147_length_3076_cov_6.417080"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PK502_10375"
     CDS             complement(<1..304)
                     /locus_tag="PK502_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01262.1"
                     /translation="MSDKFFPIPIEQLLGIILHELDSNQSILGIPSKLFFSPQKSNRL
                     ATEIFGQKIATPLGVAAGPHSQMAQNIIAAWLMGCRYIELKTIQTLDELEVSKPCID"
     gene            398..1072
                     /locus_tag="PK502_10380"
     CDS             398..1072
                     /locus_tag="PK502_10380"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPH01263.1"
                     /translation="MTIKVKKNTSAIILAAGKSERMGYPKFMLRCADGQTFLQKLVSG
                     FLLFGCRQVVVVLNEHEYQKVLLQIPEFPSEVLLVENSHPEWGRFYSLKLAVDRIKPI
                     DYIYVGNIDNPFVDAELLGLLYHSAAQADYLMPEYKGRGGHPILISQKIAEDIKTRTT
                     YDYHLKEFLSSYSSLRVEVNNPLVLANINTIDDYRQYFGDWKLSKYQAPIQNSYDGFD
                     VKKLTE"
     gene            complement(1657..2550)
                     /locus_tag="PK502_10385"
     CDS             complement(1657..2550)
                     /locus_tag="PK502_10385"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPH01264.1"
                     /translation="MKPNLTAQSTNKLIDEKDITKLSRFFKGKAGEKFLKFLMHLLAI
                     DKVNWVYDHSFDYSGPEFTSRLLSDIGVDYKVGNPERLEKLPKGAFITIANHPYGGLD
                     GIMLIDILARIRPDFKIMANKQLSLIKTLASNFISVLPKTDANKGYSPFNFQSIRDTL
                     NLIRSGHAVGFFPSGAISDFKFRNLRVSDREWQDSIIRLIQKAQVPILPIRFFDRNSI
                     FFYFLGLIDWRIRSLRLPHEIFNKQGQCQRIGVGEIITVGEQAKYPDSEMFGMFLRNM
                     VYGMPLPSAFIQKSALCLPTK"
     gene            complement(2703..>3076)
                     /locus_tag="PK502_10390"
     CDS             complement(2703..>3076)
                     /locus_tag="PK502_10390"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPH01265.1"
                     /translation="FGNSLNPTIDTGQLEGAIVQGIGWATIEELMFNNKGTLLSNSLS
                     TYKVPDIHFAPKSIRCIPLEADGPEQAIMKSKAIGEPPFMYGIAAYFAIQNAIMEFNP
                     SYQPSYDLPLTPEKVLMGLYS"
BASE COUNT          857 a          634 c          712 g          873 t
ORIGIN      
        1 tgtcgatgca tggttttgaa acctccagct cgtcgagcgt ctggatggtt ttgagctcta
       61 tgtacctgca ccccataagc cacgcagcaa ttatgttctg cgccatttgg gagtgtggcc
      121 cagctgcaac cccaaggggg gtagctattt tttgcccgaa tatttccgtg gccaaccggt
      181 tgcttttttg aggactgaag aatagctttg atggaatccc caaaattgat tgattgctgt
      241 ccagctcgtg cagaattatg ccaagtagct gctctattgg aatcgggaaa aatttatcgc
      301 tcatatcaca tgattattac ccacaaagct aaaaaataaa cgttgaatga gctatttttg
      361 cccttaatca agctgaataa ttagaaaaaa tcgacagttg accatcaaag taaagaaaaa
      421 tacatcggca attatactag ctgctggaaa atctgaaagg atggggtacc ccaaatttat
      481 gcttaggtgt gccgatggcc aaactttttt gcaaaaattg gtatcggggt ttttgttgtt
      541 tgggtgcagg caggtggttg tagtgttgaa cgagcatgaa taccagaagg tattactcca
      601 aatccccgaa tttcccagcg aagtgctgct ggttgagaac agccacccgg aatggggacg
      661 gttttactcc cttaagctgg cagttgatag aattaaaccc attgattata tatatgttgg
      721 caacatcgac aacccttttg tagatgctga gttacttggc ctgctttacc attcagcagc
      781 acaggccgac tatcttatgc cggagtataa gggtagggga ggacacccca ttctcatctc
      841 tcagaaaatt gctgaggata tcaaaacacg cacaacttac gactaccacc taaaggagtt
      901 cctttcaagc tattctagct tgcgggttga ggtgaacaat ccgcttgttc tggctaatat
      961 caataccatc gacgattacc gtcagtattt tggtgattgg aagctgagca aataccaagc
     1021 accaatccaa aattcttatg atggttttga tgtgaaaaag ttaacggaat aggcagccta
     1081 aaggttattc cagttgcccc atccttttac attacttcac aaataaacat gttaattctg
     1141 atgccccacg ggtgaaaatg ggatgtgggc agggtgctag tacaaacgct gggatggtgt
     1201 gtcctgggat ttgcactgag atttccagca gagccgcctc gttgaaaatg gcaaccattg
     1261 gcccgataat aacagcactg ggtgattgga tttttatcac ggcgcatata acgactgcac
     1321 gtcataatat tcccttttcg cgccatttgt agctggccga aatgaggatt accagcccga
     1381 tggctgtggg gatgctacca ctgaacggaa tttttaggtt atgcaggaag ctacctagaa
     1441 ctatttccga tgatgcccac attgtgccaa gcaataaagc cctaactcac ttgatactta
     1501 gctgtatgga agtgttttgt agcgggctgc tagctaaaaa agtggcggat ttttcccttt
     1561 gggccatgct gtaatgaaat ttttatcttt taaccagcca agctgccatt actattgaat
     1621 tgcctattgg gttgataaat aaaattgatt gcagtcctac tttgtaggaa gacatagcgc
     1681 tgatttttgt ataaatgctg agggtaaagg cataccgtac accatgtttc gaagaaacat
     1741 gccgaacatt tcggagtcgg ggtatttggc ttgttcgcca acagtaatta tttctccaac
     1801 cccgatgcgt tggcactgac cctgcttatt aaatatttcg tggggtagcc ttaagctacg
     1861 aattcgccag tcaatcagcc caagaaagta gaagaaaatc gaattacgat caaaaaaacg
     1921 tatgggtaag attggaacct gtgccttttg tattaagcgg ataatgctat cttgccactc
     1981 gcggtcgctt actcgaaggt tacggaactt gaaatcggaa atagcccccg aagggaaaaa
     2041 acccacagca tggccacttc taattaagtt caacgtgtcg cgaatactct gaaagttaaa
     2101 cggtgagtag cccttatttg catctgtttt aggaagaacg gaaataaagt ttgaggctag
     2161 tgttttgatt agcgaaagct gcttgttggc cataatctta aaatcgggac gaattcttgc
     2221 caaaatatca atcagcataa ttccatccag gcctccatat ggatggttgg caatggtgat
     2281 gaaagctcct ttgggcaatt tttcaagccg ttcggggtta ccaaccttgt agtcaacccc
     2341 aatgtcgcta agcagcctgc tggtaaactc tggccctgag tagtcgaagg agtgatcata
     2401 aacccagtta accttatcaa ttgcaagcag gtgcattaag aatttcaaga acttttctcc
     2461 agctttaccc ttgaaaaatc tggatagttt agtaatgtct ttttcgtcaa tgagcttatt
     2521 agtcgactgg gcggtgaggt taggcttcat tttagaacaa aatcatttag aggaaaacaa
     2581 aaatacacat attatctgca tttggaaaat ggacaacgag tgtgtaagac ttccgcttaa
     2641 ctttcgactc agcctgatat ttgggcaata cagccttgct cattggttga tgaatacgat
     2701 ttctaggagt acagccccat cagcaccttt tcaggtgtaa ggggtaaatc gtagctgggc
     2761 tggtagctag ggttgaactc cataatggca ttttgaatgg cgaagtaggc ggcaattccg
     2821 tacatgaagg gtggttcgcc aatagctttc gatttcataa tggcctgctc tggaccatcc
     2881 gcttcaagcg gaatgcacct gatacttttc ggggcaaaat ggatgtcagg aaccttgtag
     2941 gttgataggc tattggacag gagggttcct ttgttgttga acataagctc ttcaatggta
     3001 gcccatccaa ttccctgaac aatagcgcct tcaagctgtc cggtatcaat ggtggggtta
     3061 aggctattgc caaaat
//
LOCUS       DAOPHX010000196         3035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_45003_length_3035_cov_3.154027, whole genome shotgun sequence.
ACCESSION   DAOPHX010000196 DAOPHX010000000
VERSION     DAOPHX010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45003_length_3035_cov_3.154027"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PK502_10395"
     CDS             complement(<1..274)
                     /locus_tag="PK502_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01266.1"
                     /translation="MTNKLLVAILFLCVFIQNSFSQNNDQINDLYLKIKKIEDRESDI
                     SKMKSRRGVSDRKYLIKSIIDSGEIISKHKIRYYRSGIKKERIKYYK"
     gene            complement(663..2051)
                     /locus_tag="PK502_10400"
     CDS             complement(663..2051)
                     /locus_tag="PK502_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HPH01267.1"
                     /translation="MFSKQTYTNRRNQLRAQFKSGILLFIGNDESGMNYEDNTYRFRQ
                     DSSFLYYFGINRPGLYAIMDIESGHDYVYGDDLTIDSIVWMGSQPTIHEMAAGFGVEN
                     TGPLSNFYQKVKTYPLASVHYLPPYRPEHYLKLMDIFGISKQEASARASVPFITAVAE
                     QRNVKTDEEVEEIDHAVSITAEMHMAAMRYALPGMTEAQVAARIYEVALAAGGDISFP
                     IIATINGQTLHNHYHGNTISEGQLFLVDAGYETPKGYAGDMSSTFPVGRTFNERQKEI
                     YQITLAAHNGAIDKLKPGIHFRDVHLHAARIIFDGLKNMGFAKGNTDDAIANGAHALF
                     FPCGVGHLMGLDVHDMENLGEQYVGYCGAPKSTQFGLKSLRLARPLKPGFVVTIEPGI
                     YFIPELIDLWQQNGTNRDYINFDRVNEYRHFGGIRNEEDVLVTPTGHRVLGKPLAKTV
                     EEVEALRAEAFF"
     gene            complement(2144..>3035)
                     /locus_tag="PK502_10405"
     CDS             complement(2144..>3035)
                     /locus_tag="PK502_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPH01268.1"
                     /translation="TRPTTTVIRRLADGKQLMEIEKSDITDLINMGWKPAEQFSLAAD
                     DAKTPLYGLIIKPTNFDSTKKYPVIDATYTGPHTISTPQTFSSSVLNMNLSLAELGFV
                     VVYIDGRGSAYRSKAFHDVSYARLGFGLVDHVYAIKKLAQKCAYLDTNRVGIYGHSAG
                     GYDATRGLLLFPEFYKVAVSSAGDHDHRMEKEWWPELYQGFPVDTQYQNQSNITNAGK
                     LKGHLLLATGDLDNNVNPSATIKLAEELTKANKDFELLILPNEDHGGCFWNKYFIRKR
                     WDFFVKHLLGQEPPREYKIQ"
BASE COUNT          731 a          703 c          695 g          906 t
ORIGIN      
        1 ttttataata tttaattctc tcttttttta tgccgctacg atagtatctt attttgtgct
       61 ttgaaataat ttctcctgag tcaattatgg atttaatcaa atattttcgg tctgaaacac
      121 ctcttctact cttcatttta gaaatatcag attctctgtc ctcaattttc tttattttaa
      181 gatacaagtc gttaatctga tcgttattct gagaaaatga attttgaata aaaacacaga
      241 gaaataaaat agcaactaaa agtttgtttg tcatattgta atagtttttg ttatgtcaat
      301 ttaactggtc tataactcgt ttataggtgc cactccatag cgcccttatt tccatatttg
      361 gatgtataga cgccataaag ttgcgttcta tttctataaa caaatttaga atttatttta
      421 caactcatca aatgggcttc taattcactg caaacttttc tggcaagcct gggtttaaac
      481 atctgtagcc cgaccacttt tatggccggg tagcaaccgg ataaaaccgc gggtggccag
      541 ctgcgttcag gtattcggaa cggttggccc tacagatttt acagcggtgc acttaccagg
      601 tacctgcggg ttatatgcct gctcggggag gttatgtccc ccaaggcttc gggtaatcct
      661 ttttaaaaaa atgcctctgc cctgagggct tccacttcct caaccgtctt ggccaagggt
      721 ttgcctagca ccctgtggcc ggtaggggtg accagcacgt cctcttcgtt ccgaattcct
      781 ccgaagtgcc tatactcgtt taccctgtcg aagttgatgt agtccctgtt tgtcccattt
      841 tgctgccata ggtcaataag ctcagggata aagtagatgc cgggttcaat ggttaccaca
      901 aacccgggtt taagagggcg tgcaagcctc aacgatttta acccgaattg cgtgctttta
      961 ggagcaccac agtagccaac atactgctca cccaggtttt ccatatcgtg aacatcgagc
     1021 cccatcaggt ggccaacccc acatggaaag aaaagtgcat gtgctccatt tgcaatggca
     1081 tcatcggtgt taccctttgc aaagcccata ttctttaggc catcaaaaat gatacgggct
     1141 gcgtgtaggt gtacatctct gaaatggata cctggtttta gcttgtcaat ggctccattg
     1201 tgagcggcca gggttatttg gtaaatttcc ttttgcctct cgttgaaggt tctgccaaca
     1261 gggaaagtgc tgctcatatc gcccgcataa cccttaggag tttcataccc ggcatcaaca
     1321 aggaaaagct ggccttcact tatggtgtta ccatggtagt ggttatgcag cgtttgcccg
     1381 ttaatagttg ctattatcgg aaagctgata tctccgccag cagcaagggc tacttcgtaa
     1441 attctggcag caacctgagc ctctgtcatt cccggaaggg catagcgcat ggccgccatg
     1501 tgcatttcgg ctgtaatgga tacggcatgg tcaatttcct caacctcttc gtcggtttta
     1561 acgtttcgct gctcggcaac ggctgtaata aatggaacgc ttgcccttgc cgatgcctcc
     1621 tgtttgctga ttccaaaaat atccatcagc ttcaggtagt gctcgggccg gtagggcgga
     1681 aggtagtgga cggaagctaa agggtaggtt tttaccttct ggtaaaagtt ggataaaggg
     1741 ccggtatttt caacgccaaa tccagcagcc atttcgtgaa tggtaggttg cgaccccatc
     1801 cacacaatgc tgtcgatggt taaatcgtcg ccataaacat agtcgtgccc actctctata
     1861 tccattatag cgtaaaggcc tggcctgtta atgccaaaat agtaaagaaa tgagctatcc
     1921 tgacgaaaac ggtaggtgtt gtcctcatag ttcattccgc tctcatcatt tccaatgaaa
     1981 agtaggatac cggatttaaa ttgtgcccgc agctggttgc gacggttggt ataggtttgc
     2041 ttgctgaaca tgctggttga atttttagtt ggttattatt acactcacag gctgggtcga
     2101 aatttgtaat ctcctgctcc gcccggcgtg gatgtataac aggttactga attttatact
     2161 cccttggcgg ctcttggcca agtaggtgct tcacaaagaa atcccatctt ttcctgataa
     2221 agtacttgtt ccagaaacag ccaccgtggt cttcattagg aaggattagc agctcaaagt
     2281 ccttattggc tttggttagc tcctcggcaa gcttaatggt tgcagaaggg tttacgttgt
     2341 tatccaaatc gccggttgcc agcagcaaat gcccctttag cttgccggca ttggtaatgt
     2401 tcgactggtt ttgatactgg gtgtcaaccg gaaaaccctg gtagagctct ggccaccact
     2461 ctttttccat acgatggtcg tgatcgccag ccgacgaaac agccaccttg taaaattccg
     2521 gaaataggag caatcctcgt gtggcatcgt agcctccggc cgaatggccg tatattccta
     2581 ccctattggt gtcgaggtag gcacattttt gcgcaagttt tttaatagcg taaacatggt
     2641 caaccaaacc aaagcctagc cgtgcgtagg acacatcgtg aaatgccttg gagcggtatg
     2701 cgcttccacg cccgtcaata tataccacta caaaacccag ctctgccaac gacaggttca
     2761 tgtttaacac gctgctcgag aatgtttggg gagtgctgat ggtgtgagga ccggtatagg
     2821 ttgcgtcgat aacggggtat tttttggttg aatcgaaatt cgttggcttg atgattagtc
     2881 cgtaaagcgg tgttttcgca tcgtcggcag ctaagctgaa ctgttctgcg ggcttccacc
     2941 ccatgtttat caggtcggtg atatcggatt tttcgatttc catcagctgc ttcccatcag
     3001 ccaaccttcg aatcaccgtg gtggttggcc tcgtg
//
LOCUS       DAOPHX010000197         3004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_45641_length_3004_cov_4.166158, whole genome shotgun sequence.
ACCESSION   DAOPHX010000197 DAOPHX010000000
VERSION     DAOPHX010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45641_length_3004_cov_4.166158"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            597..998
                     /locus_tag="PK502_10410"
     CDS             597..998
                     /locus_tag="PK502_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01269.1"
                     /translation="MEISIKGIRGKDFVIGPTDSLCRKLSMLIEGHCTIGVQEAIKKY
                     GYTEQRYYQLLRKYKEGGARALMNSKPGPDKPAVRTREVVNQIIRLRFLDPLSGADVL
                     AQKLNQMGCRVSRRSVERTITEYGLQKKRMP"
     gene            1511..2371
                     /locus_tag="PK502_10415"
     CDS             1511..2371
                     /locus_tag="PK502_10415"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPH01270.1"
                     /translation="MKRKQPQEPSRKMVQTFFALDAQTGQPIGCGIGSPGANTAQATM
                     GLLKMVGPVSKDALVLADKEHFTERLIRSIAHNGDFELLVPAVSTARVRAAERSQTYL
                     REWAGYAVAETRFNFAGRKEKYRLISQREGETAASYRYKSFLAISEKSATELLTEMYR
                     ERWAIEDFFNFDGAMGFDRASTFNLNIRYGKMSLALLAQAATHDLRKRLPKPYSRWNA
                     THLADAVFTKMDGDIRVKDDAIVVTCYNAPQELGLQNNYQGLPAKLMSEGINPKMPWL
                     YDYKLDFRFK"
     gene            complement(2364..2879)
                     /locus_tag="PK502_10420"
     CDS             complement(2364..2879)
                     /locus_tag="PK502_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01271.1"
                     /translation="MDWHEFVQPIFMGTLQSEMVRGKEVFYFENDTQWLKYSQFRNID
                     PDLTAFTGKQFLPPSKQNFGIFLDSSPDRWGRTLLQRKEAIVARNEGRTPRHLWETDY
                     LMGIYDGNRMGALRFKFSKDGDFMDNNPHMATPPWTSLRELEHASWQIELEEPNKSAI
                     FYGVFILVNLI"
BASE COUNT          781 a          729 c          769 g          725 t
ORIGIN      
        1 ttatttgata atcaataggt catttttgaa gcgttcatgt ggtgcacaaa acaggggtac
       61 catatccacc gaggagggaa aagagttttt atccaactac cgatcaggac tctacggata
      121 cacctattta gagtaaactg gcaatgcatg gttacatccg ctttttcccg caaagaagaa
      181 tttttttaac cacaaaggac ggttattcgg ttattcggtt attcgctaat tcggctattc
      241 gtaaattcgt gatcccgacg tgtcagcgat ttttttagat acattggttt tagttttaag
      301 catcattttt aatctgaatt tctatcgagt tgaattactt tcattgccct cccttcaata
      361 cggtggtccc ttccctgaat ttgcagcacg agccatccct gatgctaaat tagaataagg
      421 cgatactatt tcttcgatta gctctaacgc cacaactcga atttacatat atatttatac
      481 atgtttgcat tgtggtaatt attatttacc ttattgctct ggtattcaaa attatttcta
      541 cctttaccgc catatataaa tttagtctat ttttgttcaa ctcataggta aaagccatgg
      601 aaatctcaat caaaggcatc agggggaaag attttgtcat tggcccaacg gatagcttat
      661 gcaggaagct gtccatgctc atcgaggggc actgcaccat cggggtgcag gaggccataa
      721 agaagtacgg gtataccgag cagaggtact accagctgct gcggaaatac aaggagggcg
      781 gcgcccgtgc cctcatgaac agcaagcccg gcccggacaa gcccgccgtc aggaccaggg
      841 aggtagtgaa tcagatcatc cggctccgct ttctggaccc cctctccggc gctgatgtgc
      901 tcgcccagaa gctgaaccag atggggtgta gggtgagccg gcgcagcgtg gagaggacca
      961 tcacggagta cgggcttcaa aaaaaacgca tgccctgaac ccccgggagg agaaacgcag
     1021 gcaggacgtt gaggtacacc aaacgaagcg ggagctagtg cgggccagcg tcagcccccg
     1081 gggggtagag cggctgttca gggagagcct ctcaaaaaag ataagcggta ccctggtcgg
     1141 tttatggttc ctggccgctg agcatctacg cctgggcagc tgggacctga tcaagggcta
     1201 caccgggggt gccgatgcgg accttgaacc ccgcatagcc atgcagctag tcaatgaggc
     1261 cgcgctgtgc agcaaccgca taaggaggag caattacatt acccatcagg ggtttgagct
     1321 gctcaacggg ctggggttcc tggtgaccga caagcaggcg cacgacctgc tcaaccggca
     1381 cacggtcagc gaagcccaat cgttacagga agcattggct gccatgagga tgaaccatgg
     1441 ccattaccgg gggcgcttag tggccataga cccccaccgc attgtgtcca cctcccaacg
     1501 ggtcatgccg atgaagagga agcagccgca ggagccctca agaaaaatgg tgcagacctt
     1561 ttttgccctg gatgcccaaa ccgggcagcc gatagggtgt ggcataggtt cgcccggcgc
     1621 caatactgcc caggcgacca tgggcctgct gaagatggtg gggccggtga gtaaggatgc
     1681 gctggtactg gccgacaagg agcacttcac cgaacggtta atccggagca tagcgcacaa
     1741 cggcgacttt gaactccttg tccccgcagt atcaacagcg agggtccgag cggcagaacg
     1801 ctcccaaact tacctgcggg aatgggccgg gtatgcggtg gcggaaacga ggtttaattt
     1861 tgcggggcgt aaagaaaaat acaggctgat ctcccagcgt gagggggaaa cagccgcaag
     1921 ctaccggtac aaatcgtttt tggccatatc cgaaaaatct gcaacagagc tgctaacgga
     1981 aatgtacagg gagaggtggg ccattgaaga cttttttaat ttcgatggcg ccatgggctt
     2041 tgaccgggct tcaacgttta acctgaacat acgctatggc aagatgtccc tggccctact
     2101 ggcccaagcg gcaacgcatg atctaagaaa gaggttgcca aagccgtaca gccgatggaa
     2161 tgcaacgcat ttggctgacg cagtatttac aaaaatggat ggggatataa gggtgaaaga
     2221 cgacgcgatc gtggtaacct gttataatgc cccacaagaa ttagggttgc aaaacaacta
     2281 tcagggccta ccggcaaagc tgatgtcgga agggattaac cccaaaatgc cctggctcta
     2341 tgattacaag ttagatttta ggttcaaata agatttacta aaatgaatac cccgtaaaaa
     2401 atcgctgact tgttgggttc ctctagttct atttgccaac tggcatgttc caattccctc
     2461 aatgatgtcc agggaggagt agccatatgg gggttgttat ccataaaatc gccatccttt
     2521 gaaaacttga agcgcaatgc gcccatacgg ttgccatcgt atatccccat caaataatcg
     2581 gtttcccata agtgtcgggg agttctcccc tcattacgag ccacaatggc ctccttgcgt
     2641 tggagtaaag ttcgccccca tctgtcgggt gatgagtcga ggaaaatacc aaagttttgt
     2701 ttgctcgggg ggaggaactg cttgccggtg aaggccgtta aatcgggatc gatgttgcgg
     2761 aattgagagt atttaagcca ttgagtgtca ttctcgaagt agaaaacctc tttgcccctc
     2821 accatctcgc tttgaagtgt acccatgaaa attggctgaa cgaattcatg ccaatccatg
     2881 taaacatata tatttctttt ggctaacatt tttacctttt cgaaaccctt tccttcacag
     2941 tcaatttggc atcctgtaaa attctaccca gctcatcgtc ctttgccact tttatcaaat
     3001 cctt
//
LOCUS       DAOPHX010000198         2932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_47272_length_2932_cov_4.455335, whole genome shotgun sequence.
ACCESSION   DAOPHX010000198 DAOPHX010000000
VERSION     DAOPHX010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47272_length_2932_cov_4.455335"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PK502_10425"
     CDS             <1..372
                     /locus_tag="PK502_10425"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPH01272.1"
                     /translation="EIDSPEVYFDPETKIFSISGISHPENAKEFYQVIMEWVDEFYEY
                     MKNKKPCKIIVDMNFKYINSSSYKYLRELLIKMSNFKNSHFDIEVVWNYQEEDEDLLT
                     EGIVLFELPEINLPYRCVSYR"
     gene            668..1756
                     /gene="hydE"
                     /locus_tag="PK502_10430"
     CDS             668..1756
                     /gene="hydE"
                     /locus_tag="PK502_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HPH01273.1"
                     /translation="MAFIEELLAKESYTKSELVELLQVTGDGRAKLFSKAAGIKEKYL
                     GNKVYLRGLVEMSNVCRKNCLYCGIRSGNQKVNRYNLTDDEIVDAAIFAWKNKYGSIA
                     LQAGEISTREFATRVEGLLGKINAATYDELGITLSLGEQNEDTYRRWFQKGGIRYLLR
                     IESSNQELYKKLHPNDSLHDYKTRIDCLKSLRRVGYQVGSGVMIGLPFQTLEHLADDL
                     LWMQEMDLDMVGMGPYVEHPDTPLFEYRDQLLPVAERFDLSLKMTALIRIMMKNINIV
                     APTALQAIDPLGREKAIKVGANVVMPNITPGAFRNDYKLYENKPCVDDAADDCKNCLD
                     ARIQLAGHEVGYGEHGDSPHFYQRKNAQ"
     gene            1884..2603
                     /locus_tag="PK502_10435"
     CDS             1884..2603
                     /locus_tag="PK502_10435"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HPH01274.1"
                     /translation="MRTTGRKNITCNSRVVLGALLVILSILLLFNNFDILPPAWHSYV
                     FSWQTLLAIVGLILIVPQNTRVTGIVLATAGILLLLAKIYVFPNGLKKLFWPAIFLIT
                     GVLIIVNNRKQLLPGKVDQTNRKDDYVDDLSIFGGSEQRFKTDNFMGGRITNIFGGST
                     IDLSESRLAPGASNLNVLCIFGGSKIIVPAGWKLRIEAVSIFGGISDKRKQIVATDNE
                     SSELVITGITLFGGCEIKSYL"
     gene            2666..>2932
                     /locus_tag="PK502_10440"
     CDS             2666..>2932
                     /locus_tag="PK502_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01275.1"
                     /translation="MDGSNCEVAGDFFCDTPADPKLNFADDVNESCVYTGGRIDAHGD
                     AYNPATDLIMPYARHNCRTRFSSEQLNSVNYWANQSIRTCFSHMY"
BASE COUNT          909 a          602 c          637 g          784 t
ORIGIN      
        1 gaaatagact cccctgaagt ttactttgac cccgaaacca agattttttc catttcgggc
       61 atctcacacc ccgagaatgc caaagaattt taccaggtta tcatggagtg ggttgacgag
      121 ttttacgagt acatgaaaaa caagaagcct tgcaagatta tagtggatat gaactttaaa
      181 tatatcaact cttcatcgta caagtaccta agagagctgc tcatcaaaat gtctaacttt
      241 aaaaacagcc actttgatat tgaggttgta tggaactacc aggaagagga tgaagacctg
      301 ctaaccgagg gcattgttct tttcgaatta cccgaaataa acctgcccta ccgatgtgta
      361 tcataccgtt agcataatcg gccaactcac aacccggcta cgctgcccag tactgcatta
      421 catttgacca ggccaacatt ttaatgaatg ctgtcaatca tccatttatt aaggatgaaa
      481 tataaatggt caaaaatcca atttttatac ctttgtgctg ttgcagccaa ccattttcat
      541 gggattttca gcccaatatt tgatttaccc cggcaaaatc gaagttgaaa gaaggctgtc
      601 agccaaataa cagggctatg ggatagcatg ggctctacca cttgaaaaat taaagaatac
      661 aaagcttatg gcatttatag aggaactttt agcaaaagaa tcatacacaa aaagtgagct
      721 ggtagaactc ttgcaggtta ccggtgatgg aagagctaag ctctttagca aagcggccgg
      781 aataaaagaa aagtatctgg gtaataaggt ttacctccgt ggactggttg aaatgtcgaa
      841 tgtatgtcgt aagaattgct tatactgtgg tatcagatcg ggtaaccaaa aggttaatcg
      901 ctacaacctt acggacgatg aaatagtgga tgcggcaatt tttgcctgga agaataaata
      961 cggaagcatt gccctgcagg caggtgaaat tagcacccgc gagtttgcaa cgcgggttga
     1021 aggcttgctc ggtaaaatta atgctgcaac atacgacgag cttggcatca ccctttcctt
     1081 aggtgaacag aatgaggaca cataccgacg gtggtttcaa aagggaggaa tacgctatct
     1141 gctgcgaatt gagagttcaa atcaggagct ctacaaaaag ctgcacccta acgatagcct
     1201 gcacgattac aaaacaagaa tagattgcct taaaagcctc cgtcgggttg gctaccaggt
     1261 aggctcgggc gtaatgatcg ggttgccatt ccaaaccctt gaacatctgg ccgacgacct
     1321 tctctggatg caggagatgg acctcgacat ggtgggcatg ggcccctacg tggagcatcc
     1381 tgatactccg ctcttcgagt accgggacca gctgctacca gttgccgagc gtttcgacct
     1441 ctcgctcaaa atgacggcgc taatcaggat aatgatgaaa aatattaaca ttgttgcgcc
     1501 aaccgcccta caagccatag atcctcttgg aagggaaaaa gccattaagg tgggggccaa
     1561 cgtggtgatg cccaacatta ctccaggagc atttcgtaac gactacaagc tatacgagaa
     1621 taaaccctgc gttgacgatg ctgctgatga ttgcaaaaat tgtctggatg cccgcataca
     1681 gcttgccggg catgaagttg gatatggcga acatggcgat tcaccgcact tctaccagag
     1741 aaaaaatgcc caataaattt agctgtataa attctgacgt gccgtgtaaa taaccacaaa
     1801 caaataaata attatcgcta atcccaattg aacaagaaaa tatcaaaata atataaataa
     1861 ctaacatcta acactttata gatatgagaa caacaggtag aaagaacata acctgcaata
     1921 gccgggttgt tttaggtgca ctgctcgtca tactatccat tctgctgcta ttcaataatt
     1981 tcgatatcct accgccggcc tggcattcgt atgtattttc atggcagacc cttctagcaa
     2041 tagtggggct gattctaatt gttcctcaaa acaccagagt aacagggata gttttggcaa
     2101 cagctggaat tttacttctg cttgctaaaa tttatgtttt cccaaatggc ttaaaaaagc
     2161 tattctggcc agcaattttt cttatcaccg gggttttaat tatagtaaac aaccgtaaac
     2221 agcttctacc cggtaaagtt gaccaaacca acaggaagga tgactatgtt gacgacctgt
     2281 caatttttgg agggtccgag cagcggttta aaactgataa tttcatgggt gggagaatta
     2341 ccaacatatt tggcggatct acaattgacc tatcggaatc aaggctggca cccggagcaa
     2401 gtaacttaaa tgttctctgt atttttggtg gttccaaaat aattgtacct gccggctgga
     2461 agctcaggat agaggcagtt tcaatttttg gaggaatatc tgataaacgt aagcaaatcg
     2521 ttgccacaga taatgaaagt agcgagctgg ttattacagg gataactctt tttggcggat
     2581 gtgaaattaa aagctatttg taaagcttca cctttttgga ctaccgcaca cacataatga
     2641 ttttgaaata agccgtaatg aatacgtgga tggttcaaat tgtgaggtag ctggtgattt
     2701 cttttgtgat actcctgctg atccaaaact taattttgcc gacgatgtta atgagagttg
     2761 tgtatataca gggggcagga tagatgccca tggcgatgca tataatcccg ccacagacct
     2821 aattatgcca tatgctcgac ataattgtag aacaagattt agctcagagc agttaaattc
     2881 agttaattac tgggcaaatc aatcaattag aacctgtttt tctcatatgt at
//
LOCUS       DAOPHX010000199         2886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_48321_length_2886_cov_3.984458, whole genome shotgun sequence.
ACCESSION   DAOPHX010000199 DAOPHX010000000
VERSION     DAOPHX010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48321_length_2886_cov_3.984458"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PK502_10445"
     CDS             <1..924
                     /locus_tag="PK502_10445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPH01276.1"
                     /translation="SQDQQFTQFYSSPLFLAPSFTGSIVGHRVVTNYRNQWTSIPGAY
                     NTFSISWDYNSPAFRSGFGIIAMRDQAGAGNLGNTKVGALYSYDIIPTPDWHIRPGLG
                     FYVQQLSIDFGKLIFSDQLTSTPMPPTSVTQPGKQAVWDIDVSTSVVAYSDFIWFGAA
                     WDHMLRPVATFYDEDSRVPYKYSFFGGYRYVTKGFLLSKVEESITFSFNFRMQDIYRQ
                     LDLGLYWFSEPFTFGVWWRGMPKGQTRSRIDALAFMLGYRWESISVGYSYDFTISKLG
                     LGSGGSHEISLIYLFKMNPKRHWKAMPCPTF"
     gene            complement(978..1103)
                     /locus_tag="PK502_10450"
     CDS             complement(978..1103)
                     /locus_tag="PK502_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01277.1"
                     /translation="MDSPQGRIGKPLHNLRTSSFTSGLKIDGYVLPKGLIHVGSE"
     gene            1778..2191
                     /gene="rsfS"
                     /locus_tag="PK502_10455"
     CDS             1778..2191
                     /gene="rsfS"
                     /locus_tag="PK502_10455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPH01278.1"
                     /translation="MAGKKGNADETLQLLKCIVDAIEEKKGLDVVSIEIGELQNAVCD
                     YFVICHANSTTQVDAIAGNIEFKTLELQNQRVYRSSGYENAIWIVLDYIDIVVHVFLS
                     EWRNYYKIEELWADGKLCRYSDPDDDFSSMQPATF"
     gene            2244..>2886
                     /locus_tag="PK502_10460"
     CDS             2244..>2886
                     /locus_tag="PK502_10460"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HPH01279.1"
                     /translation="MSNNKNSGNQMPGDRKGQVPKPPRFNVYWIYILIILGVFLIQFF
                     FSGKSPVVTSWQEVKNQMLANDEVSKIVVVRNMGKVEVYLKEDKLTKYKDRLGEGMGA
                     VPKSGPHFYFTIGSVETFEKQLVEAQQGKPDAQIIYPEYIDQHNYFADILTWVIPILL
                     IVGLWVFFFHRMTKNASGGAAGPGNIFSVGKSKAQLFDKESNQKIDFKDVAGLE"
BASE COUNT          769 a          662 c          664 g          791 t
ORIGIN      
        1 tcccaggacc agcagtttac acaattttac tccagcccat tattccttgc cccctccttt
       61 acgggcagca ttgttggtca tagagtagta actaattata gaaaccaatg gacatctata
      121 cccggtgcgt acaatacctt tagcatctct tgggactaca attctcctgc tttccgaagt
      181 ggttttggta ttatagccat gcgcgaccag gccggagcgg gaaacctggg gaacaccaag
      241 gttggggcgc tttactccta cgacatcatt ccaacgcccg actggcatat acgacctggt
      301 ttaggatttt acgttcagca gctatccatt gatttcggga agctaatttt cagcgatcag
      361 ctgacctcca cacccatgcc acccacatcg gttacgcagc caggtaaaca ggcagtttgg
      421 gatattgatg tatccacctc ggtggtagcc tactccgact ttatctggtt tggtgcagcg
      481 tgggaccaca tgcttcggcc agtggcgaca ttttacgacg aggattcacg cgttccctat
      541 aaatattcct tttttggtgg ataccgatat gttaccaagg ggtttctgct tagcaaggtg
      601 gaggagtcca tcaccttctc gttcaacttc cgaatgcagg atatataccg ccaactcgac
      661 ctgggtctat actggttttc cgaaccgttt acatttggtg tttggtggcg cggcatgcca
      721 aagggccaaa ccagaagcag gatcgatgca ttggcattca tgttgggata caggtgggaa
      781 tccatcagtg taggctatag ctacgatttt accatttcca agctgggtct aggctcgggt
      841 ggctcgcacg agatttcgct tatctacctt ttcaagatga atccgaaaag gcactggaag
      901 gccatgcctt gcccaacatt ctaaagggag ggaaatacag gtaaaagtta atgatgataa
      961 ttttgactgt cacccgttta ttcagaccca acatggataa gccccttggg cagaacgtat
     1021 ccatcaattt taagccctga agtgaaggag ctggttcgca aattgtgaag gggtttccct
     1081 atcctgccct ggggagagtc catcacagac tgccaatctt ttaaatcttt agccttgctc
     1141 ttatgtattt ttattaccgt tgcgatgcaa aggtctaaaa ttatgctttt gagcggccgc
     1201 tacttacatc gccgaaactt ccgtggtcac caaaaaaccc aaggctttgc cctgggctag
     1261 gctgtttcgc ccctaggggc tcttgtttcc acacactcca aactcttcgt ttcaaaacta
     1321 ttccgcccct tcagggcttg taaaaatcgt tcgctccata caaggggctg cgccccttgc
     1381 tgcatattac gccccttcgg ggctaaaacc accggacaag ttccgaaatc cgccagcagg
     1441 cggacaagtt cttgaatccg tcaggagccg gacaagttcc gccgtaggcg gacaggttct
     1501 ggaatccgcc aggtaaaaga caagttccgc cggtaggcgg acaagcctga accctgagca
     1561 gttcgacagg tgttatagtg agtagttcga ctggctcact ataacaccag tcgcactaat
     1621 cactgtagca acttttgaag ggtgaatgcc tcaagccatg gttcatttaa gctattttta
     1681 gctattcagg ccaaattgcc aatgccaaaa catccacctg tttttcacgg ccaatgaaaa
     1741 ttgttttaac tttgctggct aaactaatat tgtattaatg gcaggaaaaa aaggaaatgc
     1801 tgacgaaacg ctacagctgc ttaaatgcat tgttgatgcc attgaggaaa aaaaaggatt
     1861 ggatgttgtt tcgattgaaa ttggcgagct tcaaaacgct gtttgcgact actttgtaat
     1921 ttgccacgca aactccacca cgcaggttga tgctattgcc gggaatattg aatttaaaac
     1981 tttggaactt caaaaccagc gggtttaccg ttcatcagga tatgaaaatg caatctggat
     2041 tgtgctcgac tacatagata tagtggttca tgttttcctt tcggagtgga ggaattacta
     2101 taaaattgag gaactttggg ccgacggtaa actatgccgc tacagtgacc ccgatgacga
     2161 tttttccagc atgcaaccag ctacttttta aacttttggt tactttaatg gttatcttac
     2221 cttcaaaccg taataaatga attatgagca acaataaaaa tagtgggaat caaatgccgg
     2281 gcgatagaaa agggcaagtg cctaaaccgc caaggtttaa tgtttactgg atttacattc
     2341 ttattattct tggtgttttc ttaatacaat tctttttctc gggaaaaagt cctgttgtaa
     2401 cctcgtggca ggaggttaaa aaccagatgt tggccaacga cgaggttagc aagattgttg
     2461 ttgttcgcaa catggggaaa gttgaggtgt accttaagga ggataagctt accaagtaca
     2521 aggacaggct gggcgaagga atgggggcag tccctaagtc aggccctcat ttctacttca
     2581 ccattggctc agttgaaacc tttgagaaac agctggtcga agctcaacag ggaaaacccg
     2641 atgcgcaaat tatctacccc gaatacattg atcagcacaa ctacttcgcc gatatcctta
     2701 catgggtcat tccaatcctg ctaatagtgg gactttgggt tttctttttc catagaatga
     2761 ccaagaatgc atccggaggt gcagctggcc ccggaaatat tttttcggta ggcaagtcaa
     2821 aagctcaact tttcgacaag gagtcgaacc agaagattga ttttaaagat gtagcaggtc
     2881 ttgaag
//
LOCUS       DAOPHX010000200         2885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_48343_length_2885_cov_6.360777, whole genome shotgun sequence.
ACCESSION   DAOPHX010000200 DAOPHX010000000
VERSION     DAOPHX010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48343_length_2885_cov_6.360777"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..455
                     /locus_tag="PK502_10465"
     CDS             42..455
                     /locus_tag="PK502_10465"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HPH01280.1"
                     /translation="MTPIKVFDSSYALVTFDIERKIIKVIWNGNHTVSEYKAAIGEAL
                     NFQRVSGFPVYTYLSDIRNQVIVNPESRKWFERVAIPQAVNGGLRRAAVVYDGSVFKN
                     YYLNLILLAANVYQLPLKFFGSIEEAYEWIESFHD"
     gene            complement(452..1456)
                     /locus_tag="PK502_10470"
     CDS             complement(452..1456)
                     /locus_tag="PK502_10470"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HPH01281.1"
                     /translation="MDSLTHIVLGAVVGEAAVGKKMGNKALVYGAIAGSLPDFDVFVA
                     RFFDPVDALFVHRGFSHSLFLLVILAPLAAYAVKHFSHNPRLTMLNWLALIGLPWLSH
                     ILVDIFNTYGTAILEPFSHARISFDSVPIVDIFILLVLAAAVVVILLAKRKNVLRKMA
                     AWASLSLTIAYFGTSAIIKTCVDGETRKSLITKGATYTRIHSTPLPLTNLIWMVVVED
                     SSGYTISQKNLINGKTTHKAYLARNEGLLKGCENNNNMGKLLNFTRGFYTVEKSSNEH
                     LKIYDLRFSSLAGVYPQAFVFMFEVNCNDGKLLDHPAHPSRGIIPRNVKNLWNSLWQS
                     "
     gene            1522..>2885
                     /gene="rlmD"
                     /locus_tag="PK502_10475"
     CDS             1522..>2885
                     /gene="rlmD"
                     /locus_tag="PK502_10475"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPH01282.1"
                     /translation="MSKDKYPIIEKVRIEDIAAEGKSIARIDGRVLFVPYAVPGDLVD
                     VQLTRKRKNYMEGYVVRYHEYSPIRVEPFCRHFGTCGGCKWQNLPYAEQLKIKQRQVE
                     DQLRRIGRVNFPTVSPILGSERTTGYRNKLEFTFADRRWFSGDELAKADKLPDEPALG
                     YHIPGRFDFVFDVEECFLQPEPSNSVRLAVKNYAADHHIPFMNLRSHEGTLRNLIVRT
                     SSTGDVMVILSLRQLSSDVEQLLDFMVEKFPQVSSFNYVVNAKLNDTINDLEVVCYHG
                     NKYITEEMEGIKFRVGPKSFYQTNSEQACVLYGVVREFAGLTGDELVYDLYTGTGTIA
                     NFLAPNARRVVGMEYISEAIADAIENSKLNGITNTSFFTGDIKDLLTDDFVIHEGNPD
                     VVVLDPPRAGVHPDVLKALLKISPLRVVYVSCNPATQARDIALLDSDYAIKRVQPVDM
                     FPHT"
BASE COUNT          772 a          620 c          696 g          797 t
ORIGIN      
        1 gttgactcct tagctgtagg gtggtaactt acttaaaaaa catgacccca attaaggttt
       61 tcgattcttc ctatgctctt gtaactttcg atatcgaacg aaaaattatc aaggttatat
      121 ggaatggcaa ccataccgtt agcgagtata aggcagccat tggcgaagcc cttaattttc
      181 aaagggtaag cgggttcccg gtttacacct acttgtccga tatccgaaat caggttattg
      241 ttaatcccga gagccgtaaa tggtttgaaa gggtagccat tccccaggcc gttaatggag
      301 ggctacgcag ggccgctgtt gtttatgatg gtagcgtgtt caagaactac taccttaatc
      361 tgattctgct ggcggctaac gtttaccagc tacccttgaa gttcttcggc tctatagagg
      421 aggcttatga gtggattgaa agttttcacg attagctctg ccataggctg ttccatagat
      481 ttttaacatt acgggggatt attcccctgc tggggtgagc gggatgatcc agtaatttac
      541 catcattgca gttaacttca aacatgaata cgaatgcttg gggataaacg cctgcaagac
      601 tagagaaccg taaatcataa atttttaaat gttcattgct tgatttttcc acagtgtaga
      661 aaccccttgt gaaattcagc agcttaccca tgttattgtt gttttcacag cccttaagaa
      721 gaccttcatt tcgagccagg taagctttgt gggtagtttt gccgttaatt agattttttt
      781 ggctgatggt gtatccgctg ctatcctcaa ccaccaccat ccatattagg tttgtcagtg
      841 ggagaggcgt gctatgaatc cgtgtatagg ttgcaccttt ggttataagg cttttcctgg
      901 tttcaccgtc aacacatgtt ttaattatag ccgaagtgcc aaaataggct atggttaggg
      961 aaagtgacgc ccaggcagcc atcttacgca gaacattttt cctttttgca agtaaaatta
     1021 ccaccacggc agctgccagt acaagtagga tgaaaatatc cacgatgggt accgagtcga
     1081 acgaaattct ggcatggcta aaaggttcga ggatggctgt tccataggtg ttaaaaatat
     1141 caactaagat gtgcgacagc catggtaggc caatcagcgc caaccaattc agcatggtta
     1201 gcctggggtt atgggagaaa tgttttactg cgtaggcagc taatggggcg aggattacaa
     1261 gtagaaagag ggagtggcta aacccccgat gcacgaacag ggcatcaaca gggtcgaaaa
     1321 atctggctac aaaaacgtcg aagtcgggta aacttccggc tatagccccg taaaccaaag
     1381 ccttgttgcc catcttttta cctactgctg cttctccaac aacagcgccc aaaacgatgt
     1441 gggttagtga gtccataaca atttgctacc tttgcaccct aacagccaat gttgatgttg
     1501 gttcaaatat agcacgataa agtgagtaaa gataagtacc cgataatcga aaaggttaga
     1561 attgaggata tagctgccga aggtaaatct attgccagga ttgacggtag ggtattgttt
     1621 gttccctacg ctgttccggg cgatttggtt gacgttcagc taacccgaaa gcggaaaaac
     1681 tacatggagg gttatgtggt tcgttaccat gagtattcgc ccattcgggt tgaaccattc
     1741 tgcaggcatt ttggaacatg cggcgggtgc aagtggcaaa acctgcccta tgccgagcag
     1801 ctcaaaataa agcaaagaca ggttgaggat cagcttcgac gaattggaag ggtcaatttt
     1861 ccaaccgttt cgcccattct aggctcagaa agaactaccg gttaccgaaa caagctcgaa
     1921 tttacctttg ccgatagacg ctggtttagt ggcgatgagc tggccaaagc cgacaagcta
     1981 cccgatgagc ctgcgctggg ataccatatt cccggtcgtt tcgactttgt gttcgacgtg
     2041 gaggagtgtt tcctgcaacc cgaaccatca aactccgtta ggcttgctgt taaaaattat
     2101 gctgctgacc accatattcc gttcatgaat cttcgttcgc acgagggcac acttcgtaac
     2161 cttattgtcc gtacttcgtc aactggtgat gtaatggtta tcctgtcgtt gaggcagctt
     2221 agctccgacg tagagcagct gctcgacttc atggttgaaa agtttcccca agtctcgtcg
     2281 ttcaactacg ttgtaaacgc caagttgaac gatactataa acgatttgga ggtagtgtgc
     2341 taccatggaa ataaatacat tactgaggaa atggagggca taaagtttag ggttggccca
     2401 aaatcgtttt accagacaaa ctcggaacag gcatgtgtgc tttacggagt tgtaagggag
     2461 tttgcaggac ttaccggcga tgagctggtg tacgaccttt acaccggaac tggaacaatt
     2521 gccaactttc tagcccctaa cgcccgtagg gttgttggaa tggagtatat ttcagaggca
     2581 attgccgatg ctattgagaa ctcaaagcta aacggaatta ctaatacaag tttttttact
     2641 ggtgatatta aggatttgct tacggatgat tttgtgatac atgagggaaa tccagatgtt
     2701 gtggtgctcg atcctcccag ggctggtgtt catcccgacg tgctgaaagc actgctcaag
     2761 atttctcccc tacgagtagt ttacgtgagt tgtaatcctg caacgcaggc cagggatatt
     2821 gccctgcttg attcagatta tgccataaag cgcgtacaac cggttgacat gttcccacat
     2881 accca
//
LOCUS       DAOPHX010000201         2804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_50240_length_2804_cov_6.704620, whole genome shotgun sequence.
ACCESSION   DAOPHX010000201 DAOPHX010000000
VERSION     DAOPHX010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50240_length_2804_cov_6.704620"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PK502_10480"
     CDS             complement(<1..265)
                     /locus_tag="PK502_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HPH01283.1"
                     /translation="MLKQRLQQKLLQKLSPQQIQMIKLLEVPTMLLEQRIKKEIEENP
                     VLEEVHDAEEPENDTEASLVNDDSDTETDKNDDEFTLEDYLNDE"
     gene            complement(272..1669)
                     /gene="asnS"
                     /locus_tag="PK502_10485"
     CDS             complement(272..1669)
                     /gene="asnS"
                     /locus_tag="PK502_10485"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPH01284.1"
                     /translation="MRNSERVKVKNLLESGTVGAEVAAMGWVRTKRGNKSITFIALND
                     GSTINSIQVVFDMAVFSEDQLKNVATGACIGVRGKLVESPGSGQRVEIQASYLEVLGE
                     ADSATYPLQKKGHSMEFLREIAHLRPRTNTFGAVLRIRHALAFAIHKYFNDRGFIYLH
                     TPIITGSDAEGAGAMFRVTTLDPNNLPRTPEGKIDWSEDFFGKEAKLTVSGQLEAELG
                     AMALSEVYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYDIQDNMDLAEDFLKYLIRYV
                     LTSCADDLEFLKKMYDDELVSRLQLVVDNKFERLTYTRAVEILEKANQKWEFPVFWGA
                     DLQSEHERYLVEKHFSKPVILTDYPKEIKAFYMKQNDDGKTVRAMDVLFPKIGEIIGG
                     SQREENYQKLLSRITELGVPEKDVWWYLDTRKFGTAPHSGFGLGFERLVLFVTGMSNI
                     RDVIPFPRTPKNCEF"
     gene            2044..2427
                     /locus_tag="PK502_10490"
     CDS             2044..2427
                     /locus_tag="PK502_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HPH01285.1"
                     /translation="MSLSFNPNAFDNLIVVGDRVLIKPKNPDERTKAGLLLPPGVQEK
                     QKAHSGYVVKVGPGYPIPTIGELDEPWKKSRENARYFPLQAQVGDLAVYLHENSIEVE
                     FNNEKYVVVSFSSILLLFRDEGLLE"
BASE COUNT          771 a          648 c          584 g          801 t
ORIGIN      
        1 cctcgtcgtt aaggtaatct tcaagggtga actcatcgtc gttcttgtcg gtttcggtat
       61 cggaatcgtc gtttacgagc gatgcttcgg tatcgttttc aggttcttca gcatcatgaa
      121 cctcctccaa tacaggattt tcttcaattt cctttttaat tcgctgttca agcagcatgg
      181 taggcacctc cagcagctta atcatctgta tttgctgtgg agataacttc tgaagaagtt
      241 tttgttgtaa tcgctgcttt agcattttac tctaaaattc acagtttttg ggggttctag
      301 gaaatggaat aacatccctg atgttgctca tgccggtaac aaagagcacc agcctttcaa
      361 aaccaagtcc aaatccgcta tggggtgctg ttccaaactt acgggtatca aggtaccacc
      421 aaacatcctt ttcgggaacg ccaagttcgg taatgcgtga aaggagcttt tggtagttct
      481 cctcgcgctg tgaaccacca attatttcgc caatttttgg aaaaagcaca tccattgccc
      541 tgacggtttt cccgtcatcg ttttgtttca tatagaatgc cttgatctcc ttgggatagt
      601 cggttagaat gacgggttta ctaaagtgtt tctccaccag gtagcgctca tgttccgact
      661 gtaggtctgc accccagaat acggggaatt cccatttttg gtttgccttc tcaaggattt
      721 cgacggcgcg ggtgtaggtt agcctctcga atttgttgtc aacaacaagc tgtaacctgc
      781 taaccagctc atcgtcatac atctttttta ggaattccag gtcatcggca cagctagtca
      841 gcacgtagcg aataaggtat tttaggaaat cttcggccag atccatgttg tcctgaatat
      901 cataaaaggc catttcgggc tcaatcatcc agaactcggc cagatgccgg ggggtgtttg
      961 aattttcggc tcgaaaggta gggccaaagg tgtaaacctc cgaaagagcc attgcaccca
     1021 gttccgcctc gagttgtccc gaaacggtaa gctttgcctc tttaccaaag aaatcctcac
     1081 tccaatcaat ctttccctcc ggggttcgtg gaaggttatt ggggtcgagc gtggttacgc
     1141 ggaacatggc ccctgcaccc tcggcatcag aaccggtaat aataggagta tgcaggtaga
     1201 tgaatcccct atcgttgaag tacttgtgaa ttgcaaaagc taaagcatgc cgaatgcgaa
     1261 gcacggcgcc aaaagtattc gtacggggac gcaggtgggc aatttcccta agaaactcca
     1321 tgctatgacc ctttttctga aggggatatg ttgccgaatc agcttcgccc agcacctcca
     1381 ggtagctggc ctgaatttca acccgttgcc ctgaaccggg cgattctacg agctttcccc
     1441 taacaccgat acaagcccca gtagctacat tttttagctg atcctcgcta aaaacagcca
     1501 tgtcaaaaac aacctgtatg ctgttgatag tggagccatc gttaagcgca ataaaggtga
     1561 tactcttgtt accccgcttg gtgcgaaccc atcccattgc agctacctca gctcctactg
     1621 ttccgctctc gagcaaattt tttaccttaa ccctttcgct attcctcata atcaagaaaa
     1681 ttcaattttt aatcgaactg caaaatcacg acaaaactat aaaatagttg gctatctaag
     1741 ctcgaatttc gacgattaag aatgtcaagg ttgaattctt tcaatcaaaa tgtctaaaaa
     1801 tagagggaac agcaaccaaa tgcaaatata aacctccaaa gccggctggt ctcgcacagg
     1861 ttggttgtgc tatgccgaca tcagtaagca aacagccact tttaaaataa tgcaacggct
     1921 ggctaaaaag ttaaaaaaat catattagct aaacaagttt tatatttgca gaaatgccag
     1981 ctaaccagga ttagcctcga atgaccgaaa aacaagtatt tataaatcca tatacccaac
     2041 ccgatgtcgc tttcattcaa tcccaacgcg tttgacaacc taattgtggt tggcgatcga
     2101 gtattaataa agccgaagaa tcctgatgaa cggactaagg ccgggctgct gttgccacca
     2161 ggagttcagg agaagcaaaa agctcatagc ggttacgttg taaaggttgg ccctggctac
     2221 cccattccca caataggtga gcttgatgag ccatggaaaa aatcgaggga aaatgcacga
     2281 tacttcccgc ttcaggcgca ggtgggcgac cttgccgttt accttcacga aaactccatc
     2341 gaggtggaat ttaacaacga gaagtacgtt gttgtttcgt tttcctccat tctacttctc
     2401 tttcgtgatg aagggctttt ggagtagatg cagccattcg atttttttat caaaaaaata
     2461 cattttttcg attaatggct tatatttgca agtgagatac ttgaaatgcc aatatttatt
     2521 ggggtttaaa gtttaacctg tagtttttcc ttttaaaaca ggactaattg aactaatagt
     2581 agctggtgtt gaatagtttt atgaagcgtt tcaggatgta atacatttaa accgaattaa
     2641 accaatctgt ataactaaca gctaaacgtt ttgaagatga aaaaatcaac cttaattttc
     2701 aacattattc tctcccttgc cgtggttgcc ctctttatcc ttcattttac tcaaaaatcg
     2761 ccacgaaacc atgtttccgg ctctacccca tcagaagcaa gtgc
//
LOCUS       DAOPHX010000202         2797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_50427_length_2797_cov_5.109409, whole genome shotgun sequence.
ACCESSION   DAOPHX010000202 DAOPHX010000000
VERSION     DAOPHX010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50427_length_2797_cov_5.109409"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PK502_10495"
     CDS             <1..180
                     /locus_tag="PK502_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01286.1"
                     /translation="YFSADELAQLTQFATDTRALMHEKNLNFPELIFYNGSWIIPDNR
                     DKLPSTRLTTGQSHF"
     gene            405..2237
                     /locus_tag="PK502_10500"
     CDS             405..2237
                     /locus_tag="PK502_10500"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPH01287.1"
                     /translation="MKQRFSFLIRFFLFWLIYFIVFRAVFLVYQLPLTRGIGMGDALM
                     IFTRGIWMDTSLAGYILLLASLFMALLYFVHGRFIRTVYTTYTIILLVLFSVITISDL
                     ELYRNWGFRIDATPLLYLKTPKDAMASLKGWVILVLAIAAAIAVISSMFFYRKWVAKP
                     LNHIEKGKWWYFPIFTFIAACMIIPIRGGFGIAPMNPGKVYFSQNVYCNHAALNPVWN
                     MMYSVSKSGKMSKQYPTEIKPSEAKQLFTSMMESDSTNYVLSSNRPNIVVILLESFSA
                     KLIEPLGGKSGVTPCFNRLSEQGILFTDAFASGDRSDKGIIAVLSGFPAQPTQSIIKY
                     PTKSRKLPTISGVLAENGYNNTFYYGGDPDFANIRSFLYHAKFNRIVTQDEFPKKYRN
                     SKWGVHDEYVFNYLLTDLDSARAPFFKFFFTLSSHEPFEIPAKPKFKGSDEVNQYLSS
                     AYYTDSCLGNFFNIAQKKNWYKNTIFILIADHGHRHLGNHPNYAVEKFHIPMLWIGGA
                     LSVNPTRIDETVSQVDLASTLLNQLNLSDSAFTFSKNILSSHFTPFAYYAFNDGFGFK
                     NNTDTVIYDHAGKKYIKMSRANPKQAAQEAWAFFSTYQDTFLGY"
     gene            complement(2293..>2797)
                     /locus_tag="PK502_10505"
     CDS             complement(2293..>2797)
                     /locus_tag="PK502_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01288.1"
                     /translation="KVEMKYSNLNIEAAQAMVVLSKYSKVYLGRVNSLVLESKYDRYE
                     VGSVMNLEGESGYTDFDISNLGKKFNLTTRYGNVKIIDVSDSFQSIRFEGAYTGLKAG
                     INPNVSYRIDAEAAYGSINFSADKSSVNRIEDNTTIQVSGLVGTNSRTNAEVYIRIKY
                     GNANLNK"
BASE COUNT          781 a          655 c          526 g          835 t
ORIGIN      
        1 tacttttctg ccgatgagct ggcacagctc acccaatttg ccaccgatac tcgtgctctg
       61 atgcacgaaa aaaatctgaa cttccccgag cttatctttt acaacggcag ctggattata
      121 ccagacaata gagacaaatt acccagcact aggttgacca ccgggcaatc tcatttttag
      181 cataaatcaa agcgaataag attttcttga cgaagcattc ccttcgctgg taatattata
      241 tgctaccaac ctgcatgtat ttataaaaaa aacctgccac acgagctacc ggaaatgaat
      301 gcttaaaaat ttaaaatttt atacctgcgt ttatacctac cccttttata gctatgtttg
      361 caggattttt aattaattat taacatcaac ttaacgctaa gttaatgaaa caacgtttct
      421 cattcctgat aaggtttttc cttttctggc tgatttactt catagtcttt agagcagtat
      481 ttttggttta ccagcttccg ttaacgcggg gtataggcat gggtgatgca ctaatgattt
      541 tcacgcgtgg aatatggatg gacacttcgc tggcaggata catactgctg ctggcctcgc
      601 tgttcatggc attactatat tttgttcatg gacgttttat tcgaacggtt tacacaacct
      661 ataccattat tttgctggtt ctattttcgg ttataaccat ttcggacctg gagctttacc
      721 gtaactgggg tttcagaatt gatgccactc ccctactcta cctcaaaact cctaaagatg
      781 ccatggcctc tttaaagggt tgggtaattt tagtgctagc cattgcagcg gcaattgcgg
      841 ttatctcatc tatgtttttt taccggaaat gggttgccaa acccctcaac cacattgaaa
      901 aggggaaatg gtggtatttc ccgattttca cattcatagc cgcctgcatg attattccta
      961 ttcgcggggg attcggaatt gcaccaatga atcctggcaa ggtttacttt agccagaacg
     1021 tttactgcaa ccatgcagca ttaaacccag tatggaacat gatgtactct gtttccaaat
     1081 cgggaaaaat gagcaagcaa tacccgacgg agattaagcc aagcgaagca aaacaacttt
     1141 tcaccagcat gatggaatcc gactccacca actacgttct ctcctctaac aggcctaata
     1201 ttgtggttat cctgctcgaa agctttagtg ctaaattaat tgagcccctc ggaggaaaaa
     1261 gtggcgtaac tccatgtttt aacaggcttt cagaacaagg tatattgttt acagatgcct
     1321 ttgcaagcgg tgataggagc gacaaaggaa ttattgcggt tctttcaggc tttccggcgc
     1381 aacccacaca atcaattatt aagtatccaa ccaagtcgcg taagctaccc actataagcg
     1441 gtgtgctggc cgaaaacgga tacaacaaca ctttctacta tggaggcgac cccgattttg
     1501 ccaatatccg ttcgttccta taccatgcaa agtttaacag aattgtaacc caagacgaat
     1561 tccctaaaaa ataccgcaac tccaagtggg gtgttcacga tgagtatgtt ttcaactacc
     1621 tgcttaccga tctcgatagc gccagggctc ctttctttaa attctttttt acgctaagca
     1681 gccatgagcc ctttgaaatt ccggccaaac caaaatttaa aggtagtgat gaggttaacc
     1741 agtacctaag ctcggcatac tataccgata gctgcctggg aaattttttc aatattgccc
     1801 agaaaaagaa ttggtacaaa aacaccattt tcatactcat agcagatcat ggccataggc
     1861 acctaggaaa tcatcccaac tatgccgttg aaaaattcca tattcccatg ctgtggatag
     1921 gaggcgcgct tagcgttaat ccaacacgaa ttgatgaaac tgtttcgcag gttgaccttg
     1981 cctcaacgct cctcaaccag ctcaacctca gcgattcagc ttttactttt tccaagaata
     2041 tccttagcag ccactttact ccttttgcat actatgcctt taatgacggc tttggtttta
     2101 agaataatac cgatacggtc atctatgacc atgcaggtaa gaagtacatc aagatgagcc
     2161 gagcaaaccc caaacaggct gctcaggaag catgggcatt tttttccact taccaagaca
     2221 cctttttagg atactagtgt gcttgtcgcc ttatcaaatt aaaacgggga ggttccttgc
     2281 ctccccattt ttttacttgt taaggtttgc gttgccatac ttaatcctga tgtacacttc
     2341 agcatttgtt ctgctatttg ttcctaccag gcccgatacc tgaattgtgg tgttgtcttc
     2401 aattcggttg acagatgatt tatcggcgct gaaatttata ctaccatagg ctgcttcagc
     2461 gtcaattctg taggaaacat tagggttgat accagccttc aagccggtgt aggccccctc
     2521 aaaccgaatg ctctgaaagc tatcagacac atctattatt ttaacgtttc catacctggt
     2581 ggttaggtta aactttttgc ccaagttgga aatatcgaaa tcggtataac cagactctcc
     2641 ttccaaattc ataaccgaac caacctcata cctatcatat ttagattcaa gaacaagcga
     2701 gtttaccctt cccaagtaaa ccttggagta ctttgaaaga actaccattg cctgtgctgc
     2761 ttcaatgtta aggttgctgt acttcatttc aaccttt
//
LOCUS       DAOPHX010000203         2787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_50676_length_2787_cov_4.794656, whole genome shotgun sequence.
ACCESSION   DAOPHX010000203 DAOPHX010000000
VERSION     DAOPHX010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50676_length_2787_cov_4.794656"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PK502_10510"
     CDS             <1..847
                     /locus_tag="PK502_10510"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HPH01289.1"
                     /translation="RLKEFSPAYINITHHREDVVYMPNTAGEPVPVKVRRRPGTISLA
                     AAIHQRYGIDVVPHLICGGFDAHDIEDALIDLDFLGIQNILVLRGDADKISGRFEPKR
                     GGHQHAVELVRQVQEFNRGIYLEKLIENPKESDFCIGVGGYPEKHPEAPNIDVDIRYL
                     KEKVDAGASYVVTQMFFDNAVYFKFVERCREAGIDVPIIPGIKPISAKEQLTNLPRVF
                     NVNIPQALVQEVDRCADNRQVFDVGVEWAVAQSKELKAKGAPAIHFYTMGRAENIARV
                     AAKVF"
     gene            909..2054
                     /locus_tag="PK502_10515"
     CDS             909..2054
                     /locus_tag="PK502_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPH01290.1"
                     /translation="MIRNLPLYSSLFLLLSFAGAARGQAVFLLDSLPAKTSATDTVFM
                     ATSLNGWNPHDLMYAFSPDSLGTRILQLPSVVDTFEYKLCRGSWKTVEVSADGRDIPN
                     RLYTSYDTVKLSVGGWRDMLAKGLAVSTASENVRYLPTSMRMPQLNRNRTIRLYLPPN
                     YADGGPFPVIYMHDGQNLFDDATSFAGEWHVDEILDSLYTYRGFSAIVVAIYNDDSER
                     LNEYSPWRNDSLGAGGDGNKYVRFIVETLKPFIDKHYRTDPRSQSTAIIGSSMGGLIT
                     LYAALEYPNVFGKAAVFSPSLWFSPKVFEYIQKYRRKGDQRFYMLAGEKESERMVTDV
                     TRAADLLRAAGFNNDSYLMIKIVPNGRHTEGFWSRELGEAIRYLYEF"
     gene            complement(2174..>2787)
                     /locus_tag="PK502_10520"
     CDS             complement(2174..>2787)
                     /locus_tag="PK502_10520"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPH01291.1"
                     /translation="YALVIGISDYSGTQNDLQYCDDDATDWKNRLVSEGYSVTILLDR
                     NATKSAIESAVNSLASKSIAGNEIAFCYSGHGSRGNIVTTDLYYISSSWFKTKFSTAK
                     STKMMFCFDACQIGAMKTDLNATGRVIAVASSTSTYSYDGDASMKNGVFTYYQMDGFD
                     NLSYTYLEPDCSYACSSMKSWASARRLKVAPSYADSYSGNFDL"
BASE COUNT          702 a          619 c          706 g          760 t
ORIGIN      
        1 caggctgaag gagttctcgc ctgcctacat caatattacc catcatcgag aggatgtggt
       61 gtacatgcca aacactgcag gagagcctgt tccggttaag gtacgtagaa gacccggtac
      121 cattagcctt gctgcagcca ttcaccagcg ttatggcatt gatgtggttc cccatcttat
      181 atgtggcggt tttgatgctc acgatattga agatgccttg attgatctgg acttcctggg
      241 tattcagaat attctggtgc taaggggcga tgccgacaaa atttccggca ggtttgaacc
      301 taagcgagga ggccaccagc atgccgttga gctggttcgg caggtgcagg agttcaatag
      361 gggcatctac cttgaaaagc tgattgaaaa ccccaaggag tcagatttct gcattggagt
      421 aggcggttat cccgagaagc accccgaagc gcctaatatt gatgttgata ttcgctacct
      481 taaggaaaag gtggatgcgg gtgccagcta tgtggtaacc cagatgtttt ttgataacgc
      541 agtatatttt aaatttgttg agcgttgtcg tgaggctggc atagatgtcc ctataattcc
      601 gggcataaaa cctatcagtg cgaaggagca gctaacaaat ctaccaaggg ttttcaacgt
      661 caacattccg caggcgctgg ttcaggaggt tgaccgctgt gccgacaacc ggcaggtttt
      721 tgatgttggg gtagagtggg cggtagccca atcgaaggaa cttaaagcca agggcgcccc
      781 tgccattcac ttttacacca tgggaagggc cgaaaatata gctcgggttg ctgcaaaagt
      841 tttttagcag tagcatgtcc attagcagaa accataaaaa actaaatttg tataaaatct
      901 tgttgttgtt gatacgcaac ctgccattat acagctcttt atttcttctg cttagctttg
      961 ctggagcagc aagagggcag gcggtatttc ttctggattc cctacctgct aaaacatccg
     1021 ctaccgatac ggtttttatg gccacatcgt tgaatgggtg gaatcctcac gatttaatgt
     1081 atgcctttag ccccgacagc cttggaacac ggatactaca attaccttca gtggttgaca
     1141 catttgagta caagctttgt cggggaagct ggaaaaccgt tgaggtttcg gccgatgggc
     1201 gggatatacc caacaggctt tatacatcat atgatactgt taagttgagt gtaggggggt
     1261 ggcgcgatat gctggctaag ggtctggcag tatctactgc ttctgaaaac gtaaggtacc
     1321 tgccaaccag catgaggatg ccccagctta accggaatag gaccatcagg ttatacctgc
     1381 ctccaaacta tgccgatggt ggccctttcc cggtaattta catgcacgac gggcagaatc
     1441 tttttgacga tgccacgtcg tttgccggcg agtggcatgt agatgagatt ctcgacagcc
     1501 tatacaccta ccggggtttc tcggcaattg ttgttgcaat ttacaacgac gattccgaga
     1561 ggcttaacga gtactcacca tggcgaaacg attcgttggg cgccggtggc gatggcaata
     1621 agtatgttag gtttattgtt gaaacgttaa aaccgtttat tgataagcat taccgaaccg
     1681 acccccgttc gcaaagcaca gcaattattg gcagctccat gggcgggcta atcaccctat
     1741 acgcagcgct ggagtatcct aatgtttttg ggaaggccgc cgttttttca ccctcgcttt
     1801 ggttttcacc caaggttttc gagtacattc aaaaatacag gcgcaaaggc gaccaacggt
     1861 tctacatgct cgcaggcgag aaggagagtg aaaggatggt tactgacgtt accagagcag
     1921 ccgacttgct tagggcagct ggatttaaca atgattccta cctgatgata aaaattgttc
     1981 ccaacggcag gcataccgag ggattttgga gcagggagtt gggcgaggct attcgatatc
     2041 tttatgaatt ttaacacttt gcaagtataa catccatgtg cctttacggc atttccgctc
     2101 tgtcttatct aaagtataag caaaaaggag cgcaaaagca ctcccttttt acatcgtatc
     2161 gaatactcgt agtttacagg tcgaagttgc ctgaatatga atcagcgtaa gagggcgcta
     2221 ctttaagcct tctggcggaa gcccatgact tcatggagct gcaggcatag ctgcagtcgg
     2281 gctcgaggta agtgtagcta aggttatcaa agccatccat ttgatagtat gtgaacactc
     2341 cgtttttcat ggaggcatcg ccatcgtatg agtaggttga tgtgctcgag gcaacagcaa
     2401 taaccctgcc ggttgcattt aggtctgttt tcattgctcc tatttggcag gcatcaaagc
     2461 agaacatcat ttttgtgctc ttggcagtgg aaaacttagt cttaaaccac gagctgctta
     2521 tgtagtacaa gtcggtggta actatgtttc ccctactgcc atgtcctgag tagcaaaagg
     2581 cgatttcgtt tccggcaata gatttgctag ccagggagtt tactgccgat tcaatggcac
     2641 ttttggtagc attccgatcg agcagtatgg ttacggagta accttcgctt actagcctat
     2701 ttttccagtc ggttgcatca tcgtcgcagt actgcaggtc attttgggtt ccggaatagt
     2761 ccgaaattcc aatcaccagt gcatact
//
LOCUS       DAOPHX010000204         2785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_50735_length_2785_cov_4.399267, whole genome shotgun sequence.
ACCESSION   DAOPHX010000204 DAOPHX010000000
VERSION     DAOPHX010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50735_length_2785_cov_4.399267"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..1525
                     /locus_tag="PK502_10525"
     CDS             206..1525
                     /locus_tag="PK502_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPH01292.1"
                     /translation="MELLIYTKNNSSRLTYIARLIFNDILAIKFRITTSHEEAAAYHG
                     PLLAYNKEPVNGAIQIVPHGLLNEKGISPQNFEVLSWRNLPVFPVTSPNGDFPFDIFT
                     ASFYLVSRYEEQLPATLDEHKRYRSEQSIAVKNNFLELPVIDLWAFQLLDMLQSRFNG
                     VAGCSRKFSTMFTFDLDSAYAYRYKGFVRSALGITRSLLNLRWKELQSRARVLSGAIN
                     DPNDVYAEVARKLKSFDGTTWFIHAGSWGQHDKPISLLHPAMKKIVADLSKEFKLGIH
                     PSYKSFRKNDTLSKEINRLSGVVGMKITRSRQHYLRFRLPETYIELTKMGIHEEYSMG
                     YPDRIGFRAGTCTPFTFYDLASDETLNLKVYPFAVMDSTLIFTNNCSIGESIQTINGL
                     IDVVKSVKGLFISVWHVDYLSESYPGKGWWKVLDAVISRLKEAKGEN"
     gene            1515..2462
                     /locus_tag="PK502_10530"
     CDS             1515..2462
                     /locus_tag="PK502_10530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPH01293.1"
                     /translation="MKINFLNRQQVDEARWNACMDASVNGMIYGYSWYLDIVSPGWCA
                     LADEDYHYIFPLPRLKRNFNEIIIQPLYTQQLGLFSDSHLSPEILDEFICAIPSRYRY
                     IGINLNTLNRTERYADNTSARVTYRLDLFSSYENIQKKYCQNTRRNNLKAYALGCRVE
                     RSITPAEFLGFYSKYGVPRQRMRHYVKVRQLVESLMKRDRGELVGVYTPGSQLCAVSL
                     ICKSNGKLIYLLAASSPLGRQNRAMFALVDQVIRWYSETELVFDFEGSMIPSIARFYG
                     GFGATPCSYSSITINHLPLYARFLFIAENGIRTLARKVL"
BASE COUNT          775 a          652 c          633 g          725 t
ORIGIN      
        1 cggcaattct tatcatggcc attgccggcg gagccatcat cccgcttatc tggggtaaac
       61 ttagcgatat atattcccaa acccctcaag ttgcctacat ggtacttatt ccatcttacc
      121 ttatcatcct ctactactcc gtttgggggt ataaactacg atcatggcgt agaaggtaaa
      181 accatatcac ctgtaactac ctgtagtgga actacttata tataccaaaa ataattcctc
      241 acgcctgacc tacattgccc ggttaatctt caatgatatt ctggccataa agtttcgtat
      301 cactacctcg cacgaagaag ctgcggccta ccatggccca ctgttagcct acaataaaga
      361 gcctgtaaat ggggctatcc aaattgttcc acatggattg ctcaacgaaa aggggatatc
      421 accccaaaat tttgaagttc tatcatggag aaacttacct gttttccctg taacctcacc
      481 taatggcgat ttcccatttg atatttttac agcctcgttc tacctggtta gccgttatga
      541 ggagcagctg ccggctacgt tggatgagca taaacgctac cgctcagagc agagcattgc
      601 cgttaaaaac aactttttgg agctccccgt tattgatttg tgggcatttc agctacttga
      661 tatgctgcaa tcaaggttta atggtgtagc gggttgttcc cgtaagttca gcacaatgtt
      721 caccttcgat ttggatagcg cctatgccta tcggtacaaa gggttcgtta ggtctgcctt
      781 gggtatcacc cgctcgctgc taaacctcag atggaaggag ctgcaaagcc gggcaagagt
      841 actaagcgga gccattaacg atccaaatga tgtgtacgcc gaagttgcca ggaagctaaa
      901 aagttttgat ggtaccacct ggtttatcca tgctggaagt tggggacagc acgacaagcc
      961 catcagcctg ttacatcctg ccatgaaaaa aattgttgca gatttgtcga aggaattcaa
     1021 acttggcatt catccatcat acaaatcatt caggaaaaat gacacgctca gcaaggaaat
     1081 caacaggttg tcgggggttg taggaatgaa aattacccgc agccggcagc actacctgag
     1141 atttagactg cccgaaacct acattgaact tacaaaaatg ggtattcatg aggaatactc
     1201 tatggggtat cccgatagga taggctttag agccgggaca tgcaccccgt ttacctttta
     1261 cgatttggca agcgatgaaa ccctaaacct caaagtttat ccatttgcgg ttatggattc
     1321 caccttaatt tttacaaaca attgctccat aggtgaatcc atacagacta taaatgggtt
     1381 gattgacgtt gtaaagtcgg taaagggttt gttcatcagc gtttggcatg ttgattacct
     1441 ttccgaatcc taccccggca aaggatggtg gaaggttttg gatgcagtaa tcagccgatt
     1501 aaaggaagct aaaggtgaaa attaactttt taaacaggca gcaggttgac gaggcacgct
     1561 ggaatgcctg catggatgcc tcggtaaacg ggatgattta tggctacagc tggtacctcg
     1621 acatcgtctc gcccggctgg tgcgctttgg ctgacgaaga ctaccactat attttcccgt
     1681 tgccccgcct gaagcgcaac ttcaacgaaa taattatcca gccgctctac acccagcagc
     1741 ttggactgtt ttcggatagc cacctgtcac ccgaaatact cgatgagttt atttgcgcca
     1801 ttcccagcag gtataggtac atcggcatta acctgaacac gcttaaccga acggaaaggt
     1861 atgccgataa taccagcgca cgggttacct acagactcga cctgttctcg tcctacgaaa
     1921 acattcaaaa gaagtactgc cagaataccc gcaggaacaa cctgaaagct tacgccttag
     1981 gttgtagagt agagagaagc attacaccag ctgaattcct aggattctac tcaaaatacg
     2041 gagttccccg ccagcgtatg aggcactacg ttaaggtgag gcagctggtt gagagcctta
     2101 tgaaaagaga taggggtgaa ttggtcggag tttacacccc cggaagccag ctatgtgcgg
     2161 tatcgctcat ctgtaaaagc aatggcaagc taatctacct ccttgctgcc tcatcaccat
     2221 tgggcaggca aaatagggca atgtttgccc tggttgatca ggtaatcagg tggtactcag
     2281 aaactgaact ggtattcgat tttgagggtt ccatgatacc ttcaattgca cgtttttacg
     2341 gtggttttgg cgctactcct tgcagctact ccagtatcac aattaaccac ctgcccctat
     2401 atgccaggtt cctttttata gcagaaaatg gcattagaac cctggcaaga aaggtgctgt
     2461 agtcctattt cagcattaaa aataacacca ttcaagttga aaaaagggcg aatcaggaaa
     2521 aaatgaaggt cgtgaaccgt gagtagaata aagtttcgcc atccctgccg aagggagaat
     2581 ccgtcaggag ccggacaagc tccgccggta ggcggacaaa ttcttaaatt ttgaatttta
     2641 aatcttgaat tatcttatcc tttacccttg tcttcagccc caacggggga agggtattct
     2701 ccgcaatgct cggaaaggcc ggaatcgttg aacgttgctg gtataagtaa aacggcataa
     2761 aagcctgacc cgcaaatggg tcggg
//
LOCUS       DAOPHX010000205         2690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_53183_length_2690_cov_5.527135, whole genome shotgun sequence.
ACCESSION   DAOPHX010000205 DAOPHX010000000
VERSION     DAOPHX010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53183_length_2690_cov_5.527135"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="PK502_10535"
     CDS             <1..1594
                     /locus_tag="PK502_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPH01294.1"
                     /translation="KPVTYANMAIIVRNISGMLYTLGIRKGDRVALIGENSPNWGMAY
                     LAATTIGAVVVPILPDFSDTELENIIRHSEARVVFVSAKIYSHLKIDNLKGVEAFILL
                     NSLVPIDEETFTADTTIPYSIPDPIVNVDYCDCELPRPSEDDLAAIIYTSGTTGRPKG
                     VMLTHRNLVSNVLSTLMVQEVTEHDRLLSILPLSHTYECTIGFLIPMATGASVYYLDK
                     PPVAQTLIPAMQKVRPTMVLTVPLIIEKIFRGSIKPQLTSTALRQRLYQFSLSRRLLH
                     RVAARKLHSTFGGELRFFGIGGAKLSREVELFLHEGRFPYSIGYGMTETSPLLTGSTP
                     AQVRFRTAGYCLPGQELRLLNPNPETGEGEILAKGSNVMVGYYKEPELTQAIFYEGWL
                     RTGDLGVIDDKGYLTIKGRSKNLILSSSGENIYPEEIEDIINNHEYVADSVVYESGGK
                     LMARVHLNREAVAQYLAGIKSSAADLSKNAEELTRRILDDIKVMVNSKVARFARLSVV
                     HEQLEPFEKSATQKIKRFMYQD"
     gene            complement(1893..>2690)
                     /locus_tag="PK502_10540"
     CDS             complement(1893..>2690)
                     /locus_tag="PK502_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01295.1"
                     /translation="DIKTRDVSQVYKDLSRQESETLKDVYDKAVLGLMSTAANIVRLE
                     AKFVDFNFDLLRVYPQPLIILLTVCPVAKARLENYLLAKTNLTTFDRDLLVVMLCQSG
                     FSDQALFNKLQANEQLKPIFDFMKFKKVVDNKQTGYYKVDIKKMTKLTKQVSFIEQIR
                     QRIYYERQGHYSISLNHLLNEVQLACCILEGKDIKTYKEPDVDLFLTTKSLDNSTKTK
                     ISNLFNRRNNNPVSHPGSDSRVAWSVDKNEYYDYKEFVAKTMETITK"
BASE COUNT          736 a          574 c          552 g          828 t
ORIGIN      
        1 aaagcctgtt acctacgcca acatggcgat tattgtcagg aacatttccg ggatgctcta
       61 cacccttggg attaggaagg gcgatagggt agccctcata ggcgagaata gcccaaactg
      121 gggtatggct tatttggcgg caaccacaat aggggcggtt gtagttccca ttcttcccga
      181 cttttcggat acagaattgg aaaacatcat acgccatagc gaggcgaggg tagtttttgt
      241 ttcagccaaa atttattctc accttaaaat cgacaatctt aaaggcgttg aggccttcat
      301 cctgctcaac agtttagttc ctattgacga ggaaacattt acagccgata ctactattcc
      361 ctactccata cccgacccta ttgtaaacgt tgattactgc gattgcgagt taccccgccc
      421 tagcgaggac gacctggcag caattatcta tacctcaggc accactggta ggcccaaggg
      481 ggtaatgctt acccaccgga accttgtttc taacgttcta tccaccttga tggttcagga
      541 ggtaacggag catgacaggc tactctccat tctcccttta tcgcacacct acgagtgcac
      601 cattggtttc ctaattccaa tggcaacggg tgcatcggtt tactacctcg ataaaccacc
      661 tgtagcccaa acacttattc cggccatgca aaaagttagg cctacaatgg tgcttacggt
      721 tccgttaatt attgaaaaaa tatttcgagg tagcatcaag ccccagctaa cctcaactgc
      781 cctaaggcaa cgtctatacc agttttcgct aagtcgcagg ctccttcatc gggttgctgc
      841 gcgaaaactc catagtactt tcgggggtga gcttagattc tttggaattg gcggagcaaa
      901 actttcgcgt gaggttgagc tattcctgca cgaaggccga tttccctact ccataggcta
      961 tggaatgacg gaaacatcgc cgctgcttac cggtagcact cccgcacaag taaggtttag
     1021 aacggcaggc tactgcctac ccgggcagga gctgaggttg cttaatccca accccgaaac
     1081 cggtgagggc gaaattcttg ctaagggttc caacgttatg gttggctact ataaagagcc
     1141 cgagctaacc caagcaattt tttacgaggg ttggttaaga accggagacc tgggagttat
     1201 agatgacaag ggatatttaa ccattaaggg acggtctaag aatttgatac tgagttcttc
     1261 gggcgaaaat atataccccg aggaaattga ggatattatc aacaaccatg aatacgttgc
     1321 cgattccgtg gtttacgagt cgggaggcaa gctgatggca agggttcacc tcaaccggga
     1381 agcagtggcg caatacctcg ccggcattaa atcctcagct gcggacctat ccaaaaatgc
     1441 cgaagagctt accagaagaa ttcttgacga catcaaggtt atggtgaact ctaaagtggc
     1501 caggtttgcc cggctaagcg ttgttcacga gcagcttgaa cctttcgaaa aatcggccac
     1561 ccagaaaatt aagagattca tgtatcagga ctagtcattg tagaactcgt cggatgccgt
     1621 ttccgtttcc aggttgaggt gtgttctaga attgacaatg atggagttgt aattgcagat
     1681 tcccatgctt tttcctgcaa tttttagttt gcctactttc agataaatat tttaggtcgc
     1741 ggttttggca agttcgaccg tttttgagca ttcattccct gaaacatatt tctgactttt
     1801 ttttattata ccgattaagt cggtggcttt tgtcattttt tgtttcattc gtcaaattgg
     1861 ccataacgcg tgttataggg ctttttttat tatcattttg ttattgtttc catagttttt
     1921 gcaacaaatt ctttataatc ataatattca tttttgtcaa cagaccaagc aactctcgaa
     1981 tcactacctg gatgcgaaac aggattatta ttcctcctat tgaataaatt agatattttt
     2041 gtttttgtag aattgtcgag gcttttcgta gttaagaata aatcaacatc aggctcctta
     2101 tatgttttta tgtctttgcc ttctaaaata caacaagcca attggacttc gtttaataaa
     2161 tgatttaatg aaattgaata atgtccttgt cgttcataat aaatccgttg tctgatttgt
     2221 tcaataaaac tgacttgctt agtaagtttt gtcatttttt tgatgtcaac tttatagtag
     2281 cctgtttgct tgttatctac aactttctta aacttcataa aatcgaaaat tggctttagt
     2341 tgttcattgg cttgtagctt attgaataat gcttgatctg aaaaacctga ttgacataac
     2401 ataaccacca ataagtctct atcaaaagtt gtcaaatttg tttttgcaag taaataattt
     2461 tctagtcttg cttttgcgac aggacaaaca gtaagtaaaa taattaaagg ttgtgggtat
     2521 actcgaagta aatcaaaatt aaagtcaaca aacttagctt caagccttac gatgtttgcc
     2581 gctgttgaca ttaatcctaa tacggcttta tcataaacat cttttagggt ttcactttct
     2641 tgtctagaca aatctttgta aacctgcgac acatctctag ttttaatatc
//
LOCUS       DAOPHX010000206         2683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_53352_length_2683_cov_5.671994, whole genome shotgun sequence.
ACCESSION   DAOPHX010000206 DAOPHX010000000
VERSION     DAOPHX010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53352_length_2683_cov_5.671994"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            471..1133
                     /locus_tag="PK502_10545"
     CDS             471..1133
                     /locus_tag="PK502_10545"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPH01296.1"
                     /translation="MTTLEKVLELKAKVKHLMNAEVADAMRREGIIYKVNYGVSIPEL
                     KALAKPYLGDHELALELFREEIRECKLLATMIDDPELITGEQLDEWANDFTNPEIVEQ
                     VCGNLFWRTGYALSRSIEWCLDDDELFQKAGLLIIARKASDPNVGVNLLQPYIGVIEN
                     MADTMTDTILSSMSFALREIAKRSPELCGMVLSAAQRMTQLDNELAAWLGNELIYELT
                     EA"
     gene            1543..2274
                     /locus_tag="PK502_10550"
     CDS             1543..2274
                     /locus_tag="PK502_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPH01297.1"
                     /translation="MDFELTIIVPVYNEEDNLERVYQELSSYIGTAERKAKVLLVNDG
                     STDGSLAKVKEICTHDNRFFYCSFDRNYGLSAAIKAGFDYADTPWVGYIDADLQTSPQ
                     DFNLLFEYANDYDLVTGVRSMRKDSFVKNMSSLIANNIRRAFTHDGMDDTGCPLKIIR
                     TEFAKRIPMFNGLHRFLPAMILLQNGRIKQVPVRHYPRVAGKSKYHLWNRLFGPLMDC
                     FAYLWMKKKYINYHVAENNIQPPTL"
     gene            2278..>2683
                     /locus_tag="PK502_10555"
     CDS             2278..>2683
                     /locus_tag="PK502_10555"
                     /inference="COORDINATES: protein motif:HMM:NF019214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HPH01298.1"
                     /translation="MSSWIIYAIGFIAQALFSSRVFLQWIISEKHKKVLTPKLFWELS
                     LLASVLLFIYGYLRHDFPIMLGQVITYFIYIRNIQLENQWKKFPKWLRVTLFVFPFVI
                     LIYGYNNNIMDRANLFNKENIPFVLMLLGVISQ"
BASE COUNT          753 a          608 c          600 g          722 t
ORIGIN      
        1 agaagcaagg tgcatagaac aataaaaatc gaaaatagca ggtacttttc atgctttaac
       61 tttttaacaa accgcaggaa cgtatattta atcattgata gcaacgttaa tccggaatca
      121 gtgcgtccag actttgccta gaaactaact tcaaaatatc ataacactat gcttatggtg
      181 aaggttcacc catgttattt ttataagtgt aaaggagcat tacaattttc tattgataca
      241 ctgtaggaca gttaactttg ctgccagctg gacagaatgg catggattat tactgtgtta
      301 ataggtaatt taagggctat ttgttaatat tttgatattt tacacctaga agtaagcaat
      361 cttttttaat tccgtttgct gtaggtaatg gttttgagtg ggcagtagag gttgagaact
      421 cattatgggt ttagtagaaa aaatgctaat tttgaaatta aaatagctgt atgactacgc
      481 ttgagaaagt tctggagtta aaggcgaagg tgaaacatct tatgaatgcc gaggtagcag
      541 atgccatgag gcgcgagggt attatctaca aggtaaacta cggcgtatcc attcctgagc
      601 ttaaagcgtt ggcaaagccc tatttgggcg accacgagct ggcgctggag ctattcaggg
      661 aagagataag ggagtgcaaa cttctggcaa ccatgataga tgacccggaa cttattaccg
      721 gcgagcagct cgatgagtgg gcaaacgatt ttaccaaccc tgaaattgtg gagcaggtgt
      781 gcggtaacct tttctggaga accgggtatg ccctatcgcg cagcattgag tggtgtctgg
      841 atgatgatga gttgttccaa aaggccggat tgctgataat tgcccgaaaa gcttccgatc
      901 ccaacgttgg cgtaaacttg ttgcaaccct acattggcgt tattgaaaat atggccgaca
      961 ccatgaccga tactattctt tcgtccatgt cgttcgccct acgtgaaatt gccaaacgct
     1021 cacccgagct ttgcggtatg gtgctttcgg cagcccagcg gatgactcag ctggataatg
     1081 agcttgcagc atggttaggt aatgaactta tctacgagct caccgaggcc tagactattg
     1141 gaaaagacaa ggataaagca taagataaac ttgtccgcct cctgacggat tcacttttcg
     1201 actggtgtta cagtgagtag ttcggctggc tcaccataac acctgtcgaa ctgctcaggg
     1261 ttcaaacttg tccgcctacc ggcggaacgt tttaggctcc aacgggttag tgtggtgtaa
     1321 aaacttccat aaagccattt aacttttgac tttcccccgc tcgctttcaa gcccaataat
     1381 ctttttctca gggaaaactc ttgtgcatga attttgttcc cctaataaga acaacatttt
     1441 aacagcttac taaaagtgta aaacctttta tacttttaca actttgaaat agctggctat
     1501 ttttagtgaa tcctaaggca acaacggaat tatctaaaca atatggattt tgagctgaca
     1561 atcattgttc cggtttataa cgaggaggac aacctggaac gagtttacca ggagctaagc
     1621 agctacatcg gaacagctga aaggaaagct aaggtgctgt tggtaaacga tggctccacc
     1681 gacggcagcc ttgccaaggt taaggaaatc tgcacgcacg acaaccgctt cttctactgt
     1741 tcatttgacc gtaattacgg gttaagcgca gccattaagg ccggcttcga ctatgccgac
     1801 acgccatggg tcggctacat tgatgccgac ctgcaaacct cacctcagga tttcaacctc
     1861 ctgtttgagt atgccaacga ctacgacctg gttacaggcg tcaggtctat gcggaaggac
     1921 tcctttgtga agaacatgtc gtcgctcata gccaacaata tacgcagagc ctttacgcac
     1981 gacggaatgg acgataccgg ctgccccctt aaaattatca gaacagaatt cgccaagcgt
     2041 attcccatgt ttaacggcct tcaccgtttc ctcccggcca tgatactcct gcaaaacgga
     2101 cgcattaagc aggtacctgt gaggcactat ccacgcgtag ccggcaaatc gaagtaccac
     2161 ctctggaaca ggctctttgg acccctgatg gactgctttg cctacctatg gatgaaaaag
     2221 aagtacatca actaccatgt ggccgaaaac aacattcaac ctcccaccct ataacccatg
     2281 agcagctgga taatatacgc cattgggttt atagcacaag cgcttttctc aagtcgggtt
     2341 ttcctgcaat ggattatttc cgaaaagcat aaaaaggtac tcaccccaaa gctattctgg
     2401 gagttgagct tgctggcgtc ggtgctgctt ttcatttacg ggtacttgcg ccacgacttc
     2461 cccatcatgc tgggacaggt tatcacctac ttcatctaca tcaggaacat tcagctggaa
     2521 aatcagtgga aaaaatttcc caaatggcta cgtgttaccc tcttcgtttt tccgttcgtc
     2581 atcctgatat acggctacaa caacaacatc atggacagag ccaacctgtt caataaggaa
     2641 aacataccct tcgttctcat gctgcttggg gtgatatcgc agg
//
LOCUS       DAOPHX010000207         2658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_54046_length_2658_cov_3.998079, whole genome shotgun sequence.
ACCESSION   DAOPHX010000207 DAOPHX010000000
VERSION     DAOPHX010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54046_length_2658_cov_3.998079"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            599..1615
                     /locus_tag="PK502_10560"
     CDS             599..1615
                     /locus_tag="PK502_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPH01299.1"
                     /translation="MKSSQITIKDIARELGISPSTVSRALKDHPDISQETKRVVNELA
                     TKYNYKPNIIALSLRNQRSNVIGLVIPEIAHYFFSTVISGIEEVANAHGYSVMVNQSG
                     EDYKREVAACDAFLNGIIDGLIVSVSKETENYEHFQRFEDEGIPLVFFDRIIEEISAD
                     RIIIDDFDGAYQATEHLIIQGRRKIVHFSGPQNRLIGQNRLNGYLKAMRDNGVVIDER
                     LIIHCDTFQSALIETQKLIDSGTKFDSIFTVNDFTAAGAMKILIRNGIKIPQEVSVVG
                     FGDDQTSLMVEPTLTTVNQPGFEMGKKAMEQLIRRITQTRQEPPITEILKTQLIVRES
                     SRLR"
     gene            1779..>2658
                     /locus_tag="PK502_10565"
     CDS             1779..>2658
                     /locus_tag="PK502_10565"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPH01300.1"
                     /translation="MNFTQGALFTISSIIISSGFAQARSSSDTISSRKSVEAFRIESH
                     ISINGHLDEPEWQLAGFAESFTQYEPYNGASPSQPTRVKMLYDDDALYVGAWLFDSSP
                     DSIYKELGQRDNSDNLKSDAFSVYISTYNDGVNYLQFTVSASGVQTDIKVTSDEEDRS
                     WNAVWESEVSMDNRGWYVEMKIPYSALRFSKNDTTVWGVNFLRLLKRYNEKCTWNFVD
                     KSKSGFTTQSGVLVGIAGIKPPPRLSFMPYLSAYANRYPDKSGTDYQVTGGMDMKLGL
                     SQSFTLDATLIPDFGQV"
BASE COUNT          759 a          554 c          583 g          762 t
ORIGIN      
        1 caacgcccgg caacggttca ttgtttgcat tatcggtaac cgttcctgtt accgtaatgt
       61 cttgcccatt tgctaggcct tggtaaaaaa caaggactag aatgaagcaa attctcataa
      121 aaatgcgttt gttcttcata taaaatttaa tttagggttt ggttaaattt tgcagtggaa
      181 agttaaggat tgttaatcct aaaagcatta ttttcgttgg cttataggct aaaaatcacc
      241 gcaaacgata ccgcaaacgt ttgtattttt cgttgtttat atttaacttt caccgcacag
      301 gttaaggagg tgttactttt tatactagga atatcccttt atgaatgtaa gtatcaggaa
      361 tccaatgtat aacatgaagg ttgggtgcta cactttttcc ctatccagca aaacaaggat
      421 tagtggctgt tgtttttttt aattgataaa ttagtgctta attagagggg atattttaaa
      481 aacttgttca gtactaacca ttactagaat gtttgctgtg ggtttttcct aaccacagcc
      541 ggtgtgtgat gacaaatcgt tactatttgc cagctaataa tattaaaatt cggaacggat
      601 gaaatcaagc cagattacca tcaaggacat tgcccgcgag ctgggcatca gcccatcaac
      661 cgtatcgcgt gctttaaagg atcatcccga tataagccag gaaaccaaaa gagtggttaa
      721 cgagctggcc accaagtaca actataagcc aaacattata gcccttagcc ttcgtaacca
      781 gcgtagcaac gtaattggct tggtaattcc tgaaattgcc cactactttt tttcaaccgt
      841 aataagcggc attgaggagg ttgccaatgc tcatggttat agcgttatgg tcaaccagtc
      901 gggtgaggat tacaagcgcg aggttgctgc ctgcgatgca ttcttgaacg gaattattga
      961 cgggcttatc gtttcggttt caaaagaaac cgagaattac gagcattttc agagatttga
     1021 ggacgaggga atacccctgg ttttttttga taggattatt gaggaaattt cagccgaccg
     1081 aataattatt gacgattttg acggagcata ccaggctacc gagcatctca ttatccaggg
     1141 tcgaaggaaa atagttcact tttcggggcc tcaaaatagg ctgattggcc agaacaggct
     1201 taacggctac ctcaaagcca tgagggataa cggcgtggta attgatgagc ggttgattat
     1261 ccattgtgat acattccagt ctgcattgat tgaaacacag aagctaatcg atagtgggac
     1321 taagttcgat tccattttta cggtaaacga ttttacggca gctggtgcta tgaagattct
     1381 tatccgtaat ggtattaaaa ttcctcagga ggtttcggtt gttggttttg gcgacgacca
     1441 aacatccctt atggttgaac caacccttac cactgttaac cagcctgggt ttgaaatggg
     1501 caaaaaagcc atggagcagc tcattcgaag aattacccaa acccgccagg aacctccaat
     1561 taccgaaatt cttaaaaccc agcttattgt tcgcgaatcg tcccgattaa ggtagttccg
     1621 ggaaagcacg gttttaagtt ttcactccta ccacttgcgt tttcatttta aagacggaca
     1681 tttacctttt ggttacggaa agttttttac cacaattaaa ccctttgtat ccttctcgct
     1741 caaactgaaa aacattctaa caaacagcaa gagcaggtat gaattttaca cagggcgctc
     1801 tttttacaat ctcctcaatc ataatttcat caggttttgc ccaggcacgg agtagcagcg
     1861 atacaatttc tagtaggaaa tcggtagagg catttcgtat cgaatcgcat attagcataa
     1921 atggccattt ggacgaaccc gaatggcaac ttgccggttt tgctgaaagc tttacccagt
     1981 atgagccata taatggggca tcaccaagcc aaccaaccag ggtaaagatg ctttacgatg
     2041 acgatgcgct ctacgtaggt gcatggcttt tcgattcatc gcccgatagt atttacaagg
     2101 aacttgggca aagggacaat agcgataacc tcaagtccga tgcctttagc gtttacatca
     2161 gcacctataa cgatggggta aactaccttc aatttaccgt ttcggcttcg ggcgtccaaa
     2221 ccgacatcaa ggttacctcc gacgaggagg atcgctcatg gaatgccgtt tgggaaagcg
     2281 aagttagcat ggacaaccgg ggttggtacg tggagatgaa aattccctac tcagccctcc
     2341 ggttttcaaa aaatgataca actgtatggg gtgttaattt tttgcgactc ttaaaaaggt
     2401 ataatgagaa atgcacctgg aactttgttg ataaatcaaa atcgggattt accacacagt
     2461 cgggagtgct ggttggaatt gccggaatta agccaccccc caggttgtct tttatgccct
     2521 acctttcagc ttacgccaac aggtatcccg acaagagtgg taccgattac caggttaccg
     2581 gaggaatgga catgaagctg ggccttagcc agagttttac tctcgatgct acccttatac
     2641 ccgattttgg acaggttc
//
LOCUS       DAOPHX010000208         2535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_57629_length_2535_cov_6.356048, whole genome shotgun sequence.
ACCESSION   DAOPHX010000208 DAOPHX010000000
VERSION     DAOPHX010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57629_length_2535_cov_6.356048"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2112
                     /gene="pheT"
                     /locus_tag="PK502_10570"
     CDS             <1..2112
                     /gene="pheT"
                     /locus_tag="PK502_10570"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPH01301.1"
                     /translation="RGISSEGMICAEDELGLGTSHEGIMVLPGDTPVGMPARELFKLD
                     DDYVYEIGLTPNRIDAASHYGVARDLAAYLAFHSNTDTKAVKPDVSGFSIDSKSLTIP
                     VEVVNTSACRRYSAITVSNVTVAPSPEWLQKRLKSIGINPINNIVDVTNYVLHEVGQP
                     LHAFDADAITGGRVVVQTLAEGTPFITLDGVERKLSADDLMICNGSEPMCIAGVFGGL
                     SSGITAKTTRVFIESACFNPVYVRRTARKHGLSTDASFRYERGTDPNITIWALKRAAM
                     LIREVAGGQISSDIVDVYPEPLDHFRVEFNVDSACRLIGMRIPNDQIERILTSLEISV
                     IEKNDYHWVLAVPTYRVDVTREADVVEEILRIFGYDNVEIPEKVSSTLNFSKKPDNFT
                     MQSIASGFLTGNGFNEIMCNSLTKKDYYEKLTTYPARSSVEILNPLSSDLNVMRQTLL
                     FGGMESIQRNTNYRRPNLKLFEFGNCYRYNADLADVTNPLDRYSESFRIAMWMTGLME
                     PEHWMDKHNSVNFYHLKAFVHALLARFGLDVDTLSHTEAPSDLFSYGLSYLSGSAPVA
                     SFGLISSSIADSFDIKAEVFFAEIEWSALLTKLGETKISFRELPKYPEVRRDLALLLD
                     NSVSYEQVRKLAFATERKLLRQVTLFDVYQGNNLPQGKKSYAVSFILQDETRTLTDEQ
                     IDATMKRLVDAYAKQLGAQLR"
     gene            complement(2299..>2535)
                     /locus_tag="PK502_10575"
     CDS             complement(2299..>2535)
                     /locus_tag="PK502_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01302.1"
                     /translation="LIFFGSFLYQDKKERLAGFSPKFCSRLIVSKLFRPFRACKIIRS
                     IQGAAPLATYFAPLGLKPPDRFWNPPAGGQVLES"
BASE COUNT          687 a          571 c          632 g          645 t
ORIGIN      
        1 cgtgggatta gctccgaggg gatgatttgt gccgaggatg aattgggact tggaacttcg
       61 cacgaaggga taatggtttt gcccggagat acccctgttg ggatgccggc tcgtgagcta
      121 tttaagctcg acgatgatta cgtttacgaa attgggctta caccaaatcg cattgatgct
      181 gcatcgcact atggtgtagc ccgagatttg gctgcatacc tggcttttca tagcaatacc
      241 gatactaagg ctgttaagcc agacgtgtct ggttttagca ttgatagcaa aagtttgact
      301 atccctgttg aggtggtgaa tacatcggcc tgccgtcgct actcggccat tacagtaagt
      361 aatgttactg ttgcgccatc gcccgaatgg ctccagaaaa ggctaaagtc cataggcata
      421 aatcccatca acaacattgt tgatgttacc aactatgtgc tccacgaggt gggacaaccg
      481 ctgcatgcgt tcgatgccga tgccattacc ggaggaaggg tggtggtaca aacgctggca
      541 gagggaaccc cattcatcac cctcgatggg gttgaacgta aactttctgc cgacgacctc
      601 atgatttgca atggctccga acccatgtgc attgccggtg tgtttggtgg gctgtcttcg
      661 ggaattacgg ctaaaaccac cagggtgttt attgagagcg catgttttaa cccggtttac
      721 gtaaggcgaa cagcccgcaa acacggactt agcaccgatg cctcgtttcg atacgaacgt
      781 ggaacagacc ccaacattac catttgggcg ctaaagcgtg ccgccatgct catcagagag
      841 gtagctggtg gccaaatttc atccgatata gttgacgtgt atcccgaacc tttagatcat
      901 tttagggtag agttcaacgt tgatagtgcc tgtcggctta ttggaatgag gatacccaat
      961 gaccagattg aaaggattct gacatctctt gaaatttcag tcatcgaaaa aaacgattac
     1021 cactgggtgt tggccgtgcc tacctatagg gttgatgtta ccagggaggc cgatgttgtg
     1081 gaggaaattc tcagaatttt cgggtacgat aatgtggaaa ttcccgaaaa ggtcagcagc
     1141 actctgaatt tctccaaaaa gcccgataac tttaccatgc agagcattgc cagcggcttc
     1201 cttaccggaa atggtttcaa cgaaattatg tgtaactcac taaccaaaaa ggattactac
     1261 gaaaaattga ctacctatcc cgcccgtagc tcggttgaaa tactaaatcc gctgagcagc
     1321 gacttgaacg tgatgcggca aaccctccta tttgggggaa tggagagcat tcagcggaac
     1381 accaactaca ggaggcccaa ccttaagttg tttgaatttg ggaattgcta tcggtataat
     1441 gccgatttgg cggatgttac caatccgctc gaccggtaca gcgaatcgtt cagaattgca
     1501 atgtggatga ccggcttaat ggaacccgaa cactggatgg ataagcacaa ttccgttaac
     1561 ttttatcatc taaaagcttt tgttcacgct ctgcttgcac gttttgggct tgatgtcgat
     1621 acccttagcc ataccgaagc tcctagcgat ctttttagct atggcttaag ctacttaagc
     1681 ggaagcgccc ctgtggcttc ttttgggctt ataagcagct ccattgccga tagcttcgac
     1741 attaaggcag aagttttctt tgctgaaata gagtggagtg cacttcttac gaagcttggt
     1801 gaaacaaaaa taagctttcg cgagttacca aagtatcctg aggtacgccg cgatttggcc
     1861 ttgcttctcg acaacagcgt tagctacgaa caggtaagaa agttggcttt tgccaccgag
     1921 cgtaagctgc tgcggcaggt tacacttttt gatgtttacc agggcaacaa cctgccacag
     1981 ggtaaaaaat cttatgccgt tagctttata ctacaggacg agactaggac gcttaccgac
     2041 gaacagattg atgctaccat gaagcggcta gttgatgcct atgccaaaca gctgggtgcg
     2101 cagctgaggt aaccgttaac aaaaaggata aaggataaag gataaaagat aaagggtaaa
     2161 ggaaaagata aacttgtcct cctgcttgcg gattcaccct tcgacaggct cagggttcaa
     2221 atctgtccgc ctaccggcgg aacttgtccg actactggcg gattccagat tcaaaattca
     2281 aaattcaaaa ttcaatactc aagactcaag aacttgtccg cctgctggcg gattccagaa
     2341 cctgtctggt ggttttagcc ccaaaggggc gaaataagta gcaaggggcg cagccccttg
     2401 tatggagcga atgattttac aagccctgaa ggggcggaat agttttgaaa cgatgagcct
     2461 ggagcagaat tttggcgaaa agccagctaa gcgttctttc ttgtcttgat acaagaaaga
     2521 accaaagaaa atcaa
//
LOCUS       DAOPHX010000209         2514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_58303_length_2514_cov_6.098821, whole genome shotgun sequence.
ACCESSION   DAOPHX010000209 DAOPHX010000000
VERSION     DAOPHX010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58303_length_2514_cov_6.098821"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            492..761
                     /locus_tag="PK502_10580"
     CDS             492..761
                     /locus_tag="PK502_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HPH01303.1"
                     /translation="MKEGIHPQNYRLVAFKDMSNDHVFITKSAANTKDTIVIDGVEYP
                     MVKVEISNTSHPFYTGKMKLVDTAGRVDKFMNRYKKHMDNNKAKQ"
     gene            933..1664
                     /locus_tag="PK502_10585"
     CDS             933..1664
                     /locus_tag="PK502_10585"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPH01304.1"
                     /translation="MNGSFFFEIFTVAITMVAVVLVLAYRRLKRKYLRLEQQLLLSQL
                     NPHFVFNSLTAIQSYVFRNEAHLASKYLASFAKLTRLILENSRSELCSIENEVATLEH
                     YLELQSLRFSNRFDYSIYVDEKIDADSTLIPPMLTQPFIENAIEHGFNGIEDRGKLTI
                     SYLYLDKHHVEIEVKDNGVGIKRSEQANPASGKDHLSLATEITLERLKNIRHYKRLNI
                     ILTLVDTGSIDPKQHGTLVKFKIPI"
     gene            1667..2422
                     /locus_tag="PK502_10590"
     CDS             1667..2422
                     /locus_tag="PK502_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPH01305.1"
                     /translation="MFTALIVDDELNSRQTLAYLLSHFCPTVRVIGEAEGVESAYTSI
                     QKLNPDIVFLDLQLADGNGFDLLRMFEEVHFMLIIVTAYQEYAIRAFKYCALDYLLKP
                     IDVNSLVDAVDRLQHSFSSQEANRKFQVLLSNTSACSKKCKKLVLKSLEGLRVVETDE
                     IVRCESHNNTTEFILTTEQGVMVSKTLKEYEELLAPEGFIRCHQSHLINKRHVKEVLR
                     FPSPTIRMDNGDEVPVSVRKRDILRAIAGKQNG"
BASE COUNT          722 a          531 c          523 g          738 t
ORIGIN      
        1 tatacttcat cctttatagt ttaaccctta cccgtttcat ccaaacaaag cattaatatg
       61 gttagctgac aatacaatac gatacgattt ggtgtttaat cccggaagtt atcggatgaa
      121 ccctgaagtc ccccttatgc aaattcacat tgcttggctg cgacgagcac gccagtaacg
      181 ccctgttgtt catttatttt tgccggttgt cctacatagg tgtttcattt caacaagtta
      241 tctcttaacg agactcgttt cctatccaaa tttctaactt tttgaaaaaa tgttcgtgtt
      301 gacttgaaat attgattccg gattagtaca tttgcaggct gaaacgagca tggatttatc
      361 ccgaaaaata cgaggaattg tccggctgct gttgatgtta ggagaatatt tggaaggtga
      421 gcagttagtc tcttgagtag aatattacgt gtaaactatt atagcactgc ataatattaa
      481 aatttaaaca gatgaaagag ggaatacatc cgcaaaatta ccgattggtg gcattcaagg
      541 acatgtcgaa tgatcatgtt tttatcacca agtcggctgc aaatacaaag gataccatag
      601 ttattgacgg agttgaatac ccaatggtaa aggtggaaat atccaatacc tcacacccat
      661 tttacaccgg taaaatgaag ctggttgaca ctgctggtcg cgttgacaaa tttatgaacc
      721 gctacaagaa gcacatggat aacaacaaag ccaagcaata gccttatttt gttccttttt
      781 taggctaccc ctgcactgcg taggggtttt ttattggttc gttgtaaatt agtctatctg
      841 tgatttttaa atttttttgg aaactaaatg ctacctgcta attttgtagt tgtaattgat
      901 ttaccagttg gggcaatggt taaaggataa gcatgaatgg ctcctttttt tttgagattt
      961 ttaccgtagc tattacgatg gtagctgttg tgcttgtttt ggcctataga cggctaaagc
     1021 gcaagtatct tcgcctggag caacagcttt tgctttcgca gctaaatcct cattttgttt
     1081 tcaattctct aactgctatt cagagctacg ttttccgcaa cgaggcccat ctggccagca
     1141 agtacctggc tagttttgcc aagcttaccc gcctaatact cgaaaattcc cgctccgagc
     1201 tatgctcaat tgaaaatgag gttgcaaccc tcgaacacta cctcgagctg caatccctgc
     1261 gtttcagcaa ccgttttgac tactccattt atgttgatga gaaaattgat gccgacagca
     1321 cgctaattcc tcccatgctc acccagccat ttatcgaaaa tgccatcgag catggattta
     1381 atggtataga agatagggga aaattaacaa taagctacct ctacctcgat aagcatcatg
     1441 ttgaaattga agtcaaagac aatggtgtgg ggattaaaag gtcggagcaa gcaaacccgg
     1501 cttctgggaa agaccatcta tcgcttgcaa ctgaaataac cctggagagg cttaaaaaca
     1561 tcagacacta taaaaggctg aatataatct tgacgcttgt tgacaccggt agtattgatc
     1621 ccaaacaaca tggcacgctg gtcaaattta aaattcccat ttaaccatgt ttaccgccct
     1681 tatagttgat gatgagctga actcaaggca gacgcttgcc tacctgctaa gccatttttg
     1741 cccaacagtc agggttattg gagaagctga aggagttgaa agcgcctaca cctccattca
     1801 aaagctaaac ccggatatag tttttctcga tttgcagctt gccgatggga atggatttga
     1861 ccttcttcgt atgtttgagg aggtgcattt catgctcatc attgttacag cctaccagga
     1921 atacgccatt agggcattca agtattgtgc cctcgactac ctgctgaagc ctatagatgt
     1981 taacagcttg gttgatgcgg tggacaggct gcaacactcg ttttcgagtc aggaggcaaa
     2041 ccgtaagttt caggtgctac tctcaaacac aagcgcctgt agcaaaaaat gtaaaaaact
     2101 tgttcttaag tccttagagg gactacgggt tgtagaaacc gatgaaattg tacgttgtga
     2161 gtcgcataac aacaccaccg agttcatcct tactaccgag cagggtgtca tggtgtctaa
     2221 aactctgaag gagtatgagg agctattagc tcctgagggt tttattcgct gccatcaatc
     2281 gcaccttatc aacaaaaggc atgttaagga agttctccga tttccctcac ctaccataag
     2341 gatggacaac ggcgatgaag tgcccgtttc ggttcgaaaa agggatatac tcagggcgat
     2401 agccggcaaa caaaatgggt aattgttaat attcacttaa caatgtctta acaaaagcgt
     2461 aatcccccac ggctaaccat tttaacgggc tattttatct ttgctaactg tttg
//
LOCUS       DAOPHX010000210         2433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_61031_length_2433_cov_5.928091, whole genome shotgun sequence.
ACCESSION   DAOPHX010000210 DAOPHX010000000
VERSION     DAOPHX010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61031_length_2433_cov_5.928091"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PK502_10595"
     CDS             <1..549
                     /locus_tag="PK502_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01306.1"
                     /translation="TMDSASEKLKVKSKKLKEEEIEKLIDVAYEDFCENLEEFRKIYP
                     VKTICMHGSPKSAFDNRDIWKKYDYRQLGIIGEPYFDIDFDKFFYVTDTGRCWNGYKY
                     SVRDKMPQQERWVKEGLVFKTTNDIIKAAQDNKLPNQIMITVHPQRWTNKPALWLWEL
                     AAQRAKNVVKAFRISSLARQQA"
     gene            complement(701..2080)
                     /locus_tag="PK502_10600"
     CDS             complement(701..2080)
                     /locus_tag="PK502_10600"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HPH01307.1"
                     /translation="MKLHSNFAILLVATAMMACNSPKDRVEVWVSKADSTILLEQQPG
                     INFSNNQIDEVIEVDTNQKCQMINGVGAALTWSSAYVFKNYLTPETRLQLFRELFTPE
                     GIGINVVRLTIGSSDFSRGNHSYSETPDTSLSNFTLAMDMDEVVPMMKEILAVNPNIT
                     IIASPWSPPAWMKSSNSMVGGNLLKQHYGAYANYLVKYIKAMQEQGIPIYGITVQNEP
                     EFGTATYPCMLMSAQEQNEFIRYYLGPRMAQNGLTTRIILFDHNCDSPQYPISILNDS
                     ITRKYVDGSGFHLYKGEIEALCTVHEAHPDKNIYFTEQSGGGWAPNFGDNLLWYSETL
                     MVKAMRCWSKTVLLWNLALDENDGPKNNGCSNCWGVVQVPSNGAVKRNAEYYALGHFG
                     KFVLPNATRLVSTMQKVSNVAFLNPDGSKVLIAVNTSGQPVAFTVKCGGRQFIYQMGG
                     REVVTFRWK"
BASE COUNT          665 a          545 c          560 g          663 t
ORIGIN      
        1 acgatggact cagccagcga aaagttaaaa gttaaaagta aaaagttaaa agaagaggaa
       61 attgaaaaac taattgatgt agcatatgaa gatttttgcg agaatttgga ggagtttagg
      121 aaaatatacc ctgtaaaaac tatttgtatg cacggtagcc cgaaatcggc attcgataat
      181 agggatattt ggaagaaata tgattatagg caattgggga ttattggtga gccgtacttt
      241 gatattgatt tcgacaagtt tttttacgta accgataccg ggcgttgctg gaatggctat
      301 aagtactccg tacgcgacaa gatgccgcag caggagcgct gggtaaaaga aggattggtt
      361 tttaaaacta ccaacgatat tataaaagct gcgcaggata acaaactccc caaccagatt
      421 atgattaccg ttcaccccca gcgctggacc aacaaaccgg cattgtggtt atgggaactc
      481 gccgcccaac gggctaagaa tgtggttaaa gctttcagga taagctcact tgcccggcaa
      541 caagcgtagc cgccggatat tgcctgcatt cctacactgc cgcacgaaaa ccccatcgtg
      601 cggtttactt tttatcgtta aaatactcgc gtaaacactg acattctagc tggggtttgg
      661 gcgagtattc aggcatggcc acacactgca cctgataaac ctacttccag cggaacgtaa
      721 ccacctcacg tccacccatc tggtagataa actgcctgcc gccgcacttc acggtaaagg
      781 caacgggttg tccggatgta ttcaccgcaa tcagcacctt tgaaccatcg gggttcagga
      841 aagccacgtt ggacaccttt tgcatggtgg agaccagccg tgtggcgttg ggtagaacaa
      901 actttccgaa atggcccagg gcatagtact cggcattcct cttaacggct ccgttggagg
      961 gcacctgaac aaccccccag cagttggagc aaccgttgtt cttggggcca tcattttcat
     1021 caagggcaag attccacagc agaacggttt tgctccagca ccgcatagct ttcaccataa
     1081 gggtttcgga gtaccagagc aggttgtcgc cgaagtttgg cgcccatccc ccacccgact
     1141 gttcggtgaa gtagatattc ttatcgggat gggcctcgtg aactgtgcaa agggcttcaa
     1201 tttcgccttt gtagaggtga aaacccgaac catccacata cttccgagta atggaatcgt
     1261 tcagaatgct aatgggatac tgcggggagt cgcagttatg gtcaaatagt attattcggg
     1321 ttgtcaggcc attctgtgcc atccggggtc ccaggtagta gcgaataaac tcattctgct
     1381 cctgagcgct cataagcatg caggggtagg tggccgtgcc aaactccggc tcattttgaa
     1441 cagttattcc gtaaatggga attccttgct cctgcatggc tttgatgtac tttaccaggt
     1501 agttggcata agcaccatag tgctgcttaa gtagattccc tcctaccatg ctgttggatg
     1561 atttcatcca tgccggtggg ctccacggcg aggcaataat ggtgatgttg gggtttacag
     1621 ctaatatttc cttcatcatg ggaacaacct cgtccatatc catggccaat gtaaagtttg
     1681 aaagagatgt atcgggcgtt tcgctgtagc tgtggtttcc tcgtgaaaaa tcagaggagc
     1741 caatagttag cctaaccacg ttgatgccaa taccctcagg ggtaaacagc tcgcggaaaa
     1801 gctgcaggcg ggtttcagga gtgaggtaat tcttaaaaac gtaggccgat gaccaggtaa
     1861 gagccgcacc tacgccatta atcatctggc acttttgatt tgtatcaacc tcaataactt
     1921 catcaatctg gttgttggaa aaattaatgc cgggctgctg ttctagtagt atggtagaat
     1981 cggcttttga aacccagact tcaaccctat ctttcgggga gttacaagcc atcatggcgg
     2041 ttgcaaccag caagatggca aaattcgaat gcaacttcat agtgattatt tttttgtaag
     2101 tttaactaaa accctaaatt tttaggtaat agatattttc attttctcca gcagtttttt
     2161 gttggtttat taattccggc aaaagctaat ccgttaggtg caaattttat tctctttacc
     2221 gacttaatac cagggtgcat tcaaagatgc aaaattatat tttaccacaa agggcaccaa
     2281 gatgctacac aaagttcaca aagtgccatt tataaccact ttaatcccat cctttaataa
     2341 aataacatta gagttcatca ttctctttca ttttttccct gtgttctttg tgcatgcttt
     2401 gagaactttg tggttaatat tcgccacgta acc
//
LOCUS       DAOPHX010000211         2413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_61758_length_2413_cov_4.241306, whole genome shotgun sequence.
ACCESSION   DAOPHX010000211 DAOPHX010000000
VERSION     DAOPHX010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61758_length_2413_cov_4.241306"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PK502_10605"
     CDS             <1..358
                     /locus_tag="PK502_10605"
                     /inference="COORDINATES: protein motif:HMM:NF015415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01308.1"
                     /translation="SPKSIFNFEKISKRKYLDIASVNSSILACTEGNVITSCHVSAGG
                     VAPIPTYLFNTSMFMVGKTINQETFEKANTIMQAEISPISDIRGSADYKRLLLRQLLF
                     SHLSKLFPELLKVTWL"
     gene            380..>2413
                     /locus_tag="PK502_10610"
     CDS             380..>2413
                     /locus_tag="PK502_10610"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPH01309.1"
                     /translation="MNNFNVNPMNNIDSINHVTGKSIYLDDITTLKGTLHLEIFGSAV
                     AHAKILSIDLTYAARVEGVVGIFTAKDIPGENQIGGIVEDEVLLAKTEVHYQGQPIAI
                     VAATTEIAARKAISLIRVDYEELPAVTDPRVAQKNGMLIIPPRTFSMGNTDEAWCKCT
                     HIFDGRVEIGGQEHLYIETQGAYAYPTEGGFLRVHSSTQGPTAVQRTIARVLGLPMHQ
                     IEVDVQRIGGGFGGKEDQPSQFAALAALATHLLKQPVKLSLSRHDDMRMTGKRHPYSA
                     DFKIGLDKNLKIIAYETTLYQNGGASADLSPAIMERTLFHSTASYFIPNTRVTVYSCR
                     TNLPPNTAFRGFGAPQGMFVMESAIAKAAHELGVAAQTIQELNLISEKDEFQYGQVAE
                     NAYATKCFERAKEQFKIEVLQKEIKLYNSKNRFSKRGMAVMPLCFGISFTNITMNQAR
                     ALVHIYQDGSVSVSTGAVEMGQGVNTKLAQVAAITLGIGIERVKIYSTNTSRVANTSP
                     TAASTGADINGNALLIAATRLLERLKQVAAEMLSCRASEVEIKNEEVLCRGERSSTSW
                     QKLIQTAYLKRICLTENGHYSTPKIYFDKTKEKGHPFAYMVYGTGIVTTTVDCIRGTY
                     SIDEVLVVHDFGNSLNPTIDTGQLEGAIVQGIGWATIEELMFNNQGTLLSNSLS"
BASE COUNT          710 a          574 c          572 g          557 t
ORIGIN      
        1 atcaccaaaa tctatattca actttgagaa aatcagcaag cgaaagtatc ttgatattgc
       61 cagcgtcaac tcatccattc ttgcctgcac tgaggggaac gtcatcacaa gctgtcatgt
      121 ttcagcagga ggggtggctc caatacccac ctatcttttt aatacctcca tgttcatggt
      181 aggaaaaacc attaaccagg agacttttga gaaggccaac accatcatgc aggccgaaat
      241 ttcgccaatt tccgacataa gagggagtgc tgattacaaa aggttgcttc tgaggcagct
      301 ccttttttca catctctcga agcttttccc tgaactgtta aaggtgacat ggctttagca
      361 tattttgaat gaacattgga tgaataactt caacgtgaac ccgatgaaca acatagactc
      421 cattaatcat gtaaccggca aatcaattta cctggatgat ataaccaccc tgaaggggac
      481 acttcattta gaaatatttg gttcagcagt ggcccatgcg aaaattttaa gcattgacct
      541 tacttatgct gcaagggtgg agggtgttgt aggaatattc acggcaaaag atatacccgg
      601 cgagaaccag attggcggaa tagttgagga cgaagtcttg ctggccaaaa ccgaggtgca
      661 ttatcaggga cagcctattg ccattgttgc agccacaaca gagattgctg cacgcaaggc
      721 aatttcatta attagggttg actacgagga acttcctgca gttaccgatc cccgcgtagc
      781 tcagaaaaat ggaatgctca tcattccgcc ccgaaccttt agcatgggaa acaccgatga
      841 agcatggtgc aaatgcaccc atatatttga tggtagagtg gaaattggcg gacaggagca
      901 cctatacatt gaaacgcagg gtgcttacgc ttacccaaca gaggggggat ttctacgagt
      961 tcactcatca acccaggggc ctacagcggt tcaacgaact attgccaggg tgttgggatt
     1021 gcccatgcac cagattgaag ttgacgttca acgcattgga ggtggattcg gcggcaagga
     1081 ggatcagcct tcgcagtttg cagcactggc agcactggcc acacacctgc ttaagcagcc
     1141 tgtaaagctt tcgctctcgc gccacgacga catgcggatg acagggaaaa gacatcccta
     1201 cagcgccgat tttaaaattg gactcgacaa aaaccttaaa attattgcct acgaaaccac
     1261 tctttaccag aacggtgggg cttcggccga tctatcaccc gccatcatgg agcgaacgct
     1321 attccattca actgcctcct actttatccc caatacgcgg gtaaccgttt acagctgccg
     1381 gacaaacctg ccacccaaca cagcatttcg tgggtttggg gcacctcagg gaatgtttgt
     1441 aatggaatcg gccattgcta aagctgctca cgagctgggt gtagcagcac aaaccataca
     1501 ggagctgaac ctgattagtg aaaaagacga gtttcagtat ggacaggtgg ccgagaacgc
     1561 ttatgctaca aaatgctttg aaagggcaaa agaacagttc aaaatcgaag tgctccaaaa
     1621 ggaaattaaa ctttacaaca gcaaaaatag gttcagcaag cgaggcatgg ccgtcatgcc
     1681 cctgtgcttc ggcatatcat ttaccaacat aacaatgaac caggcaaggg ccttggtgca
     1741 tatataccag gatggaagcg tatcggttag cacaggagct gtagagatgg gacagggtgt
     1801 aaataccaaa ctcgcccagg ttgctgccat tactttaggc ataggaattg aaagggtgaa
     1861 aatatactct accaacacat ccagggttgc caacacgtcg cctaccgccg caagtacagg
     1921 tgccgatatc aatggaaatg ccctgctgat agctgcaacc cggcttcttg aaaggctaaa
     1981 gcaggtggcc gcagaaatgc tcagctgcag ggccagcgag gtggaaatta aaaatgagga
     2041 agttttatgt cggggagagc gcagcagtac atcctggcaa aagttgattc aaactgccta
     2101 cctgaaaagg atatgcctca ccgaaaacgg gcactactct accccaaaaa tatactttga
     2161 caagaccaag gagaaaggcc atccttttgc ctacatggtt tacgggacag gcatcgttac
     2221 taccaccgtt gactgtatca ggggcacata ttctattgac gaggtgctgg tagttcacga
     2281 ttttggcaat agccttaacc ccaccattga taccggacag ctcgaaggcg ctattgttca
     2341 aggaattgga tgggctacca ttgaagagct tatgttcaac aaccaaggaa ccctcctgtc
     2401 caatagccta tca
//
LOCUS       DAOPHX010000212         2360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_63722_length_2360_cov_4.550108, whole genome shotgun sequence.
ACCESSION   DAOPHX010000212 DAOPHX010000000
VERSION     DAOPHX010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63722_length_2360_cov_4.550108"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="PK502_10615"
     CDS             <1..1322
                     /locus_tag="PK502_10615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPH01310.1"
                     /translation="FNRLNNYCGQWIHHSNWYPDRKLRLFDRRKGCWGGINPHDRVVM
                     QKGSRVHYLKGDLLHWVLNSYEAHIDKANKFSTIAAHEYFKLGKKSSIFGIFYHSSWC
                     FFKAYVIRLGFLDGYNGFVVSSLTAYTRFLKYVKLRHLNLQHKSESLSVERIAKKEFV
                     PLTSQPASGKPIATIIITTYNQPEWLKKVLWGFSKQTFSGFEILIADDGSDEKTKAVI
                     EEFSRTGKFRLKHVWHPDMGFRKCTIINKAITQASSDYLIFTDGDCIPRNDFVETHVK
                     YARRGYFLSGGYFKLTQPVSVTITHDDITMQKPFKASWLLHQGQPFTYKLLKFTRSRF
                     LQNLLNTITTARATWNGHNVSGWKADIVAVNGYNENMQYGGLDRELGERLVNYGLKGK
                     QIRYSAACIHLDHPRPYKTQLTLDQNKQIRKDVRRNKITWTSNGIIK"
     gene            1341..2189
                     /locus_tag="PK502_10620"
     CDS             1341..2189
                     /locus_tag="PK502_10620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPH01311.1"
                     /translation="MPRPVASLIISTYKNVEFLNAVIKSVFRQTDRRVEIIISEDGEC
                     EHTKEFVSELRSPLPLRHLTQPDVGWRKNKALNRAILASTTDYLIFIDGDCMLHPRFI
                     EFHLKFASDKSILAGKRVKLDPHTSALLLNDQLVVSQMNAYLLKNFTSLKRQKAKFIE
                     EGIFINPRGPFAFIPAIRAMHQLKGCNMSFSKKAIYSINGFDEDYINPAIGEDIDLTW
                     RFKMAGFRIKSLRNLAVQYHLHHKECWTEQVINHDLMLRKQKSGSVICSNGLIKTSLG
                     NNCCQE"
     gene            2201..>2360
                     /locus_tag="PK502_10625"
     CDS             2201..>2360
                     /locus_tag="PK502_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HPH01312.1"
                     /translation="MNVSNPSLLRAAETLRSGGIILYPTDTVWGIGCDATNPAAVDKI
                     FRLKQRDDS"
BASE COUNT          700 a          537 c          514 g          609 t
ORIGIN      
        1 actttaaccg gttaaataac tactgtggac aatggattca ccattcaaac tggtatcctg
       61 acaggaagct caggcttttt gaccggagga aaggctgctg gggtggcata aaccctcatg
      121 accgggtagt tatgcaaaag ggaagcaggg tgcattatct taagggcgat ttgctccatt
      181 gggtactcaa cagctacgag gcacatatag acaaggccaa caagtttagc accattgcag
      241 cacatgaata cttcaagttg ggaaagaaaa gcagcatttt cggtattttc taccacagtt
      301 catggtgctt ttttaaagct tacgttatcc ggcttggatt tctcgatggg tataatggtt
      361 ttgtggtaag ctcgctgaca gcctacacgc gttttttaaa atacgtaaaa ttacgccatt
      421 tgaaccttca acataaatcc gagagtttga gcgtagaaag aattgcaaaa aaagaattcg
      481 tacccctcac ttcgcaacca gcatccggga aacccattgc caccatcatt attaccacct
      541 ataaccagcc ggaatggcta aaaaaggtac tctggggttt tagcaagcaa accttctccg
      601 ggtttgaaat tctcatagcc gatgacggct ctgatgaaaa aaccaaagcg gttattgagg
      661 aattttcaag aaccgggaag ttccggctaa agcatgtttg gcatcctgat atgggcttcc
      721 gtaaatgcac aattattaac aaggctatta cccaggcatc gtccgattac ttaattttta
      781 ctgacgggga ttgcattccc cgaaacgatt ttgtggagac ccatgtaaaa tatgcccgtc
      841 ggggttactt tctaagtggc ggctacttta aacttaccca gcctgtctcc gtgaccatca
      901 cccatgatga tatcaccatg cagaaaccat tcaaggcatc ctggctgctt caccaggggc
      961 aaccctttac ctacaagcta cttaagttta cccgcagcag gtttctgcaa aacctcctga
     1021 ataccataac tactgcccgt gccacatgga atgggcataa tgttagcggc tggaaagcag
     1081 atattgttgc agtaaacggc tataatgaga acatgcagta cgggggctta gaccgtgaac
     1141 ttggcgaaag gcttgtcaat tacgggctaa aaggaaagca aattaggtac agcgctgcct
     1201 gtattcacct ggaccatcca cgtccgtata aaacacaact taccctggac caaaataaac
     1261 aaatccgcaa ggatgtaagg agaaataaaa ttacctggac atccaacggt attatcaagt
     1321 aactctatac atccatattt atgccaagac cagttgcctc cctgattatt tcaacctaca
     1381 agaacgtaga atttcttaat gctgtcataa aatcggtgtt caggcaaacc gacagacgag
     1441 ttgaaattat tatttctgaa gatggggagt gtgaacatac taaagaattt gtttcggagc
     1501 ttagatcacc ccttcctctc aggcacttaa cccaacccga cgttggatgg cgaaaaaata
     1561 aagctttaaa tcgtgccatt ctggcttcga caaccgacta cctaattttt attgacggcg
     1621 actgcatgct acatccccgc ttcatagaat ttcacctaaa atttgcctct gacaaaagta
     1681 tccttgccgg aaaaagggtt aaacttgacc cgcacacgtc ggcgttgctg cttaacgacc
     1741 agcttgtcgt ttctcagatg aatgcctatt tgctgaaaaa tttcacttcg ctcaaaaggc
     1801 aaaaggcaaa attcattgag gagggtatat tcattaatcc ccgggggcca tttgcattta
     1861 taccggccat ccgggcaatg caccagctta aagggtgcaa tatgtctttt tccaaaaagg
     1921 ccatctattc cataaatggc tttgatgagg attacataaa cccggcaatt ggggaggaca
     1981 ttgatctaac ctggaggttt aaaatggcgg gttttcggat taaatcccta agaaatctgg
     2041 cagttcagta tcatcttcat cataaggaat gctggacaga acaggttatc aaccatgact
     2101 tgatgctcag gaagcaaaag agtgggtcgg tgatctgttc aaacgggcta atcaaaactt
     2161 cactggggaa taattgttgt caggaataga gccagaatgt atgaatgtta gcaacccatc
     2221 tcttcttcgg gctgccgaga ccctccgttc cggaggcatc atcctctacc caaccgatac
     2281 ggtgtggggg attggctgcg atgccacaaa ccctgcagcg gttgacaaaa tattcaggtt
     2341 gaaacagcgc gacgatagca
//
LOCUS       DAOPHX010000213         2351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_64045_length_2351_cov_4.264373, whole genome shotgun sequence.
ACCESSION   DAOPHX010000213 DAOPHX010000000
VERSION     DAOPHX010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64045_length_2351_cov_4.264373"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PK502_10630"
     CDS             <1..221
                     /locus_tag="PK502_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPH01313.1"
                     /translation="NGKATLLNPFFNTTETLEGIDAIVMAGTKAANNSLYNEIRSSLQ
                     NVYVIGDAAAPRNTGAAIADAMSLSVNF"
     gene            304..1068
                     /locus_tag="PK502_10635"
     CDS             304..1068
                     /locus_tag="PK502_10635"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPH01314.1"
                     /translation="MSKLLENKTVLVTGGGSGIGRAAALGLAREGARVVVVDVNENGG
                     NETVRLIENAEGKAVFIKANISEEVEVMNLISKVVDLYGHLDGAFNNAAISGKLGKTT
                     IDYDSSDWDALMSVNLKGTWLCMKHEIIQMLKQGYGAIVNTSSDAGLIALPGLNPAYV
                     AAKHGIVGLTKIAAIEFADRNIRVNAIAPGMTHTGMTHDLIAGAPSEREVASYLPIKR
                     VADPAEIAEAAIWLLSGKASFVTGAILSVDGGSVAD"
     gene            1123..1716
                     /locus_tag="PK502_10640"
     CDS             1123..1716
                     /locus_tag="PK502_10640"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPH01315.1"
                     /translation="MKKLFLLLGLASALTFTVGAQTSAWVIDNMHSNFGFKISHMGIS
                     LVEGEFNEFSGTITTSREDFSDAKAEILVKAASINTDVQPRDQHLRSADFFEVEKYPD
                     ITFVSTKMERTYGNNFKLTGNLTMHGITKTIVLDVTYNGQAYEEQMKVQKAGFTVQGV
                     LNRDDFGISAFSFPLPSGAPMVGREVFLTIPLELIKQ"
     gene            1794..>2351
                     /locus_tag="PK502_10645"
     CDS             1794..>2351
                     /locus_tag="PK502_10645"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPH01316.1"
                     /translation="MKAARLHELGKPLVVEEVSIPELNDDDVLVEVKATFVAPSMKDI
                     SHLGGNFIRPTLPAILGSDAVGVISKLGRRVKRLRVGQYVWVNSMLYYNTDEFALRGQ
                     EGLSDSMAFQGMFTFNPNNIHLMDEYQGGFAQFIKAPSVSIAALPDNFPLEYAVRLGY
                     MGTAYHALKRAGVHYGSTVLINGATG"
BASE COUNT          667 a          477 c          546 g          661 t
ORIGIN      
        1 ataacggtaa ggcaaccctc ttgaacccat tttttaacac aacagaaacg cttgagggta
       61 ttgatgccat tgtaatggcc ggaacaaagg cagctaataa ttcgctttac aacgaaatac
      121 gaagcagcct acagaatgta tatgttatcg gcgatgctgc tgctccgcga aacacagggg
      181 cggcaatagc cgatgccatg agcttaagcg ttaattttta aattctaatc ataggaaacc
      241 cctttccgtt agaggggttt cccttaacat ttttcgttct gactaaaaca aaaaaacaaa
      301 ataatgagca aactacttga aaacaaaacc gtgctggtaa ccggcggggg ttcagggata
      361 ggtagagctg ctgctttggg attggccaga gaaggtgcaa gggtggttgt tgtggatgtt
      421 aatgaaaatg gtggaaatga aaccgttcgc ttgatagaga atgctgaagg caaggccgtt
      481 tttatcaagg ccaatatatc ggaagaggtc gaggtgatga acctcatcag taaggtggtt
      541 gatctgtacg gacaccttga tggtgctttt aacaatgcgg caatttcagg gaaattgggg
      601 aaaactacaa ttgactatga ctcgtccgac tgggatgccc tgatgagcgt taaccttaaa
      661 ggaacatggc tttgtatgaa acatgagatc atacaaatgc tcaaacaggg ctacggtgct
      721 attgtgaata cctcatccga tgcgggatta attgccctac cgggtctcaa ccccgcttac
      781 gttgcagcta agcacggcat tgttgggcta acaaaaattg ccgccattga gttcgccgat
      841 aggaatattc gagttaacgc cattgcacca gggatgacac ataccggaat gactcatgac
      901 ttaattgccg gagcccccag cgaaagggaa gtagcttcat acctcccgat taaaagggtt
      961 gccgaccctg ccgaaattgc cgaggccgca atttggttgc tttccggcaa agcctcgttt
     1021 gtcacaggag ctatactatc agttgatggc ggctcagtgg ccgactagat ggcgcttatg
     1081 taattttaac cttactgatc attaacctta aaactatttg ctatgaaaaa attatttttg
     1141 ctacttggtt tggcttcagc cctaactttt actgttgggg cacagacttc tgcatgggta
     1201 atagataaca tgcactccaa ttttggcttt aaaatatccc atatgggtat atcactggtt
     1261 gagggcgagt tcaacgaatt ttcgggaact atcactacct caagggagga tttttcagat
     1321 gcaaaagctg aaatactggt taaagctgca agcatcaata ctgatgttca gcctagagac
     1381 cagcacctaa ggtcagcaga tttcttcgaa gttgaaaaat atcccgatat cacgtttgtg
     1441 agtacaaaga tggaacgcac ctatggtaat aatttcaagc tcactggtaa tctaaccatg
     1501 cacgggatta ccaaaaccat tgttctcgat gttacataca atggtcaggc ttatgaggaa
     1561 caaatgaagg tacaaaaagc cggatttacg gttcaaggag tgttaaaccg tgacgatttt
     1621 gggatttcag catttagctt tccattacct tctggcgccc caatggttgg gcgtgaagta
     1681 tttctcacta ttccacttga gctaatcaaa cagtaatgtt ataattcttt aatgattgcc
     1741 cttatatcat tcactattct tatcacttaa tatgaatttc tataaattat gctatgaaag
     1801 ctgcacgttt gcatgagctg ggcaaacccc ttgtggttga agaagtgtcc attcctgaac
     1861 ttaatgacga tgacgttctg gttgaagtta aggcgacctt tgttgctcct tctatgaagg
     1921 atatttcaca cctaggagga aatttcattc gtccaaccct ccctgctatt ctcggatcgg
     1981 atgctgtggg tgttatctca aagcttggta gaagggtaaa gaggctgaga gttggacagt
     2041 atgtttgggt gaattcaatg ctatactaca ataccgatga gtttgcttta aggggacagg
     2101 aagggttgag cgattccatg gcatttcagg gaatgttcac ctttaacccc aacaatattc
     2161 atctcatgga tgaataccag ggaggctttg cacaatttat taaggctcca tcggtaagta
     2221 ttgctgctct acctgataat tttcctctgg aatatgccgt acgtcttggc tatatgggta
     2281 cagcgtacca tgctcttaaa agagccgggg ttcattatgg ctctacagta ctcatcaatg
     2341 gggctactgg a
//
LOCUS       DAOPHX010000214         2076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_76245_length_2076_cov_4.933201, whole genome shotgun sequence.
ACCESSION   DAOPHX010000214 DAOPHX010000000
VERSION     DAOPHX010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76245_length_2076_cov_4.933201"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            636..2036
                     /gene="mnmE"
                     /locus_tag="PK502_10650"
     CDS             636..2036
                     /gene="mnmE"
                     /locus_tag="PK502_10650"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPH01317.1"
                     /translation="MLDDSTIVAIATPPGMGAIAVIRVSGVDSITLTDGVFKPVMAGK
                     SLLKAKPYTLHYGHIVDMERVIDEVLVSIFHAPHSYTGENAVEISCHGSVVIQQQILE
                     LLIRRGARLAKPGEFTLRAFLNGKMDLSQAEAVADLIASTSEAERRVALQQMRGGFSD
                     KLKLLRDQLLRFTALIELELDFSEEDVEFADRKQLEALVGNIHEEVSRLADSFSMGNA
                     IKQGIPVTIAGKPNVGKSTLLNRLLNEEKAIVSEIAGTTRDAIEDTIQLAGMNFRFID
                     TAGLRHTTDEVESLGIARTHSKIEQAKVVLYLIDAAESMSVGLTEVEKFQQKLTNQQT
                     LMVLLNKADRAEAQHTELLAQNIESHLHCITLIISAKSGQNINELTQNLVNIGMSAQP
                     DGNNVVVTNARHYESLVNARDSLARVLSGLKSNLTGDLLAVDIHQALYHLGSITGEIT
                     NDEVLGYIFSKFCIGK"
BASE COUNT          570 a          513 c          496 g          497 t
ORIGIN      
        1 gtagatattc attttaaaaa cgatttaaaa gtgttggccg caaaggtaga aaatttaaac
       61 ttagagcacg agtaattggt agatctaagt tacaaatgca acttttttgt taaacatggt
      121 agtggtggac acgcagccaa taccttccag cttcgcccgt ttagaaatct gctcgggcag
      181 ccatcgatcc taactaagcc gccgctcaat atagactact accctggggt gagcaccgcc
      241 gctagcgacc cttggccttg cgccgggcta acccaaaccg gtaaacgcat tatgcccgtc
      301 ggtgttatgc ttaatttact gggtaatggc gctcttgtca accccaacga ccctggctat
      361 ctcaattttt ttgtacccgg ttcccaacat ctttgaaata gtgtaccttt gttcttgcgt
      421 ttagtgtacc atcctgcagc ttatttctta agagatttag ggggcgagga tagccaccta
      481 accgtcagga acaacgaggg aaggattagc tctttcactc tcgttcttga aaaattagat
      541 tttttgggtc tgccccttaa gctaatgtcc ctaaaagttg catttgcaat gaaaatctaa
      601 gatgccaaat ggtgtaactt tgcaaaaaat atacgatgct cgacgactca accatagtgg
      661 ccattgccac accccccgga atgggggcca ttgctgtgat tagggttagc ggtgtggatt
      721 ccatcaccct gaccgatggt gtgttcaaac ctgtgatggc cgggaaaagt ctcctaaagg
      781 ctaaacccta cacccttcac tatggacaca ttgttgatat ggaacgggtg attgacgagg
      841 tgctggtaag catttttcat gcgccccact cctataccgg cgagaatgcg gttgaaattt
      901 cgtgccatgg ctcggttgtt attcaacagc aaattctgga gttgctgatt cgccgtgggg
      961 caagacttgc caaacccggg gagttcaccc tgcgggcgtt cctgaatgga aagatggacc
     1021 tgtcgcaggc cgaagcggtt gccgacctta tagcgtcaac tagtgaagct gagcgccgcg
     1081 tggccttgca gcaaatgcgg ggagggtttt cggataagtt gaagctgctg cgcgatcagc
     1141 tgctgaggtt taccgctctc attgagctag aactcgactt tagcgaggag gatgtggagt
     1201 ttgccgaccg caaacagctc gaagccctcg ttggcaacat tcatgaagag gttagccgtt
     1261 tggccgattc gttcagcatg ggcaacgcca ttaagcaggg catccccgtt accatagcag
     1321 ggaagcccaa cgtcgggaag tcaaccctgc tcaaccgcct attgaatgaa gaaaaggcaa
     1381 ttgtttcgga aatagccggc accacccgcg atgccattga ggacaccata cagctggccg
     1441 gaatgaactt caggttcatt gacactgccg gactcaggca caccaccgac gaggttgagt
     1501 cgcttggcat agcccgaact cattccaaaa ttgaacaggc caaagtggtg ctctacctga
     1561 ttgacgccgc agaaagcatg agcgttggcc tgaccgaagt tgagaaattc cagcaaaaac
     1621 tcaccaacca gcaaaccctt atggtgctgc tgaacaaggc agacagagcc gaagcccaac
     1681 ataccgagct gctagcccag aacattgaaa gccatcttca ctgtataacc ctcataattt
     1741 cagcaaaatc aggacaaaac ataaacgaac taacacaaaa ccttgtaaat attggtatgt
     1801 cagcacagcc cgatggtaac aacgtggtgg ttacaaacgc acgccactac gaatcgcttg
     1861 taaacgcacg cgatagcctt gctagggtat tatctggact aaaatcaaac ctaaccggcg
     1921 acctgttggc agtggatata catcaggctc tctaccatct gggaagcata accggcgaaa
     1981 taactaacga tgaggtgcta ggctacatat tctcaaaatt ctgtatcgga aagtaatctt
     2041 ctttcagcaa aaatattcag taggacaaca acattc
//
LOCUS       DAOPHX010000215         1978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_81728_length_1978_cov_5.785751, whole genome shotgun sequence.
ACCESSION   DAOPHX010000215 DAOPHX010000000
VERSION     DAOPHX010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81728_length_1978_cov_5.785751"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PK502_10655"
     CDS             complement(<1..727)
                     /locus_tag="PK502_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPH01318.1"
                     /translation="MSRNYHNLTSYYNYYYNAYDSYRNGERRVYKDFKYNYTLPLPML
                     LFGERGVSDMVSSDMERTLNKCTALLAKHSITVKPKRKNGSLSSKEKEFYNRYEYVRW
                     AQESWLLVGKAHTWKGDLSKAQMAFEYVIRQFPNTTTWYEAQVWLARVEILNGDFISA
                     GDRLKSVDAKRRKPTDRQFNHLLSSTWAFYHLKQHNSNEAIPFIKRAAEFTNYKAERL
                     RYTYLLAQLQQQLGNTTEAIELYD"
     gene            complement(1157..>1978)
                     /gene="purQ"
                     /locus_tag="PK502_10660"
     CDS             complement(1157..>1978)
                     /gene="purQ"
                     /locus_tag="PK502_10660"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HPH01319.1"
                     /translation="HFKAKLLKGVKLLYFNLYINVDFACKLLIPIFEKFLDEKPMKFG
                     VVIFPGSNCDRDTIYVLREVLEQEVVTLWHKETDFDADVDAVILPGGFSYGDYLRSGA
                     LARFSPIMTRIAEFASNGGYVFGICNGFQILCEAELLPGALLHNASRQFVCKNVYIAP
                     DSKIAPPTAFLPKGRVFKIPIAHGEGRYFADRETLARMRVNGQIIFRYCDETGVISEL
                     ANPNGSVENIAGVCNERKNVFGMMPHPERASDDELGNTDGRLIFESIIKFIQGNG"
BASE COUNT          571 a          436 c          428 g          543 t
ORIGIN      
        1 tgtcgtaaag ttctatagct tcggtagtgt tgccaagctg ctgctgcagc tgagctagca
       61 ggtaggtata acgaagccgt tcggctttat aatttgtaaa ctcagctgca cgtttaatga
      121 aaggaatggc ctcgttgcta ttatgctgtt ttagatggta gaatgcccat gttgatgata
      181 gcaggtggtt aaactgccta tctgtaggtt ttcgtctttt tgcatcaaca gactttagcc
      241 tatcaccagc gctaataaag tcaccgttca aaatttcaac cctggctagc catacctgag
      301 cctcgtacca tgttgtagtg tttgggaact gccggataac atactcaaag gccatttggg
      361 ctttggacag gtcgcccttc cacgtatgtg ccttgccaac caaaagccat gattcctgtg
      421 cccacctaac atactcgtac cgattataaa attccttttc cttggacgaa agtgaaccgt
      481 ttttacgttt aggctttaca gttattgagt gcttggcaag taatgctgtg catttgttca
      541 gggttcgctc catgtcggag gaaaccatat cgctaacacc acgctctcca aacagcaaca
      601 tgggaagggg aagcgtgtag ttatacttaa aatccttgta aactcgcctc tcgccgttac
      661 ggtagctatc gtatgcgtta tagtaatagt tgtagtagga ggtaaggtta tggtaattcc
      721 tgctcataaa ggtatttttc tgggttgagc aagaagcaag aactaaagtc agcatgcaag
      781 ctgccatgtt tctggtcaat aagcgcatag taccagctgt ttccccaatc aaaagcaaag
      841 tatgccgagt tgaattaaac ggaatgttga aatttatata gcccacaata ttagcaataa
      901 aagttgaatt gccctccagg gcagcctcga gcttagcaac aattaagctg tccaacaaac
      961 agtaaacttt tattccatca aacaaccata agatggtagg aaaccgttaa gaacagccac
     1021 agttcaaaaa gatattgtat gctaaaaacg tttgtggcgg taaactattg ttgtggggta
     1081 aacaaacctt ccatctcggt ctgaaagagg gactgccggg ttgtaacaaa aatggaggaa
     1141 ttatcaaatt tccaggctag ccgttacctt gaatgaactt aattattgat tcgaagataa
     1201 ggcggccatc ggtgttacca agctcgtcgt ccgaggccct ttcgggatga ggcatcatcc
     1261 caaaaacgtt cttgcgttcg ttgcaaacac cagcaatgtt ttcaaccgag ccattaggat
     1321 ttgccaattc ggagataacc ccggtttcgt cgcagtagcg aaaaataatc tgcccgttca
     1381 cccgcatacg ggcaagtgtt tccctgtcgg caaagtagcg gccttcgcca tgggcaattg
     1441 ggattttaaa gactcgcccc ttgggaagaa atgcagtagg gggtgcaatt ttgctgtcgg
     1501 gagcgatgta tacattcttg catacaaact gacggcttgc attatgtaac aaagcacccg
     1561 gtagtaattc tgcttcacag agtatctgaa aaccgttgca aatcccaaaa acatagcccc
     1621 cattgcttgc aaactcggca attcgggtca ttattggcga aaacctggcc aaagctcctg
     1681 aacgtaggta atcgccatac gaaaaaccac cgggaagtat aactgcatca acatcggcat
     1741 caaaatcggt ttccttgtgc catagagtaa ccacttcttg ctcaagcacc tcgcgcagca
     1801 cgtaaatggt gtcacgatca cagtttgacc cgggaaaaat tactactcca aacttcatag
     1861 gtttttcatc caaaaacttt tcaaatatag gtattaatag cttacacgca aaatctacat
     1921 tgatgtagag gttgaaatat agtaacttta cacctttaag taattttgcc ttaaaatg
//
LOCUS       DAOPHX010000216         1973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_82023_length_1973_cov_5.027633, whole genome shotgun sequence.
ACCESSION   DAOPHX010000216 DAOPHX010000000
VERSION     DAOPHX010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82023_length_1973_cov_5.027633"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="PK502_10665"
     CDS             complement(<1..1428)
                     /locus_tag="PK502_10665"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HPH01320.1"
                     /translation="MYRIRLWPLGIAVIALIFGSCNVEKRSDAEQVAKAMLDRMTLEE
                     KVGQMAQITLDVVGKGDSLFNSYEPFELDTAELREAIVDYHVGSVLNSSNNRARSLAA
                     WNSIVSTIQNVAIAKTRLAIPVLYGIDAVHGANYTAEATLFPQQIGMAATWNDSLVQA
                     IGSITAYETRASGIPWVFSPVLDLGADPRFPRQYEGFGEDPHLCSVLGVALVNGYQGA
                     SVSSPLKVAACAKHYLGYSAPSSGKDRTPAQLPNITLKEYHLPPFKAAVEAGVKTFMV
                     NSGVINGLPVHASHYIITELLKEELGFEGVVVSDWNDIENLYRRDRVVATEKEAIGLA
                     VNAGIDMAMIPYRYQDFCNQLVELVREGSVEESRIDDAVMRILKLKAELGLFERPTTV
                     LGDYPKAGSEEFAKVSYQAAGESITLLKNSNGILPLSKDKRYKILVAGPNANSMRTLD
                     GGWSYSWQGEKVEEFAGRYNTIFEAI"
     gene            1656..>1973
                     /locus_tag="PK502_10670"
     CDS             1656..>1973
                     /locus_tag="PK502_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPH01321.1"
                     /translation="MDDELRLIPNLSIDCVIFGWDEGVLKVLLRKELIEYKDIVHIEW
                     KLPGNHVGMNERIEDTATRVLKEQAGVENIFMKQFMVFSDLDRLRRREMDYQWIKKYG
                     LGED"
BASE COUNT          534 a          473 c          434 g          532 t
ORIGIN      
        1 aatggcctca aaaatggtgt tgtacctgcc ggcaaattct tctaccttct caccctgcca
       61 cgagtatgac catccaccat cgagggttct catggagttt gcgttggggc cggcaacaag
      121 aattttatac cttttatcct tgcttaaggg caggatgccg ttgctatttt tgagcagggt
      181 aatagactcg cctgccgcct ggtatgaaac tttggcaaac tcctcggagc cagccttggg
      241 gtaatcgccc agcactgtag ttggacgctc aaacaatcca agttcagcct taagcttaag
      301 tattcgcatc acagcatcgt caattcggct ttcctctacc gacccctccc tcaccagctc
      361 caccagctgg ttacaaaaat cctggtaacg gtaaggaatc atagccatat caattccggc
      421 atttacagcc agccctatag cctctttttc agtggccacg accctatcgc gacggtaaag
      481 gttttcaatg tcgttccaat cagaaaccac taccccttca aaaccaagtt cctccttcag
      541 tagctcggta ataatgtagt ggctggcatg aaccggaagt ccattaatta ccccggagtt
      601 aaccataaac gttttaaccc ccgcttcaac agcagcttta aatggaggca ggtggtattc
      661 ctttagcgta atgtttggca gctgagccgg agttctatcc ttgccggatg agggcgccga
      721 gtagcccagg tagtgcttgg cacaggcagc aactttcagc ggacttgata cacttgctcc
      781 ctggtagcca tttactagcg caacccccaa aacactgcac aagtgcggat cctcgccaaa
      841 tccctcatac tgtcggggga aacgagggtc ggctcctaaa tcaagaaccg gggagaaaac
      901 ccatggaatt ccacttgcac gtgtttcgta ggctgtaatg cttccaattg cctgaacaag
      961 agaatcgttc catgttgccg ccatgcctat ctgctgtgga aaaagagtgg cctcagccgt
     1021 atagttagcg ccatgaaccg catcaattcc atataaaact ggtatagcta gccgagtttt
     1081 agctatggct acattctgaa tggtagaaac tatgctgttc cacgcagcca gtgagcgagc
     1141 tcgattattg ctgctgttta gcactgaacc cacatggtag tcaacaatcg cttcccgtag
     1201 ttcagccgta tccagctcaa aaggctcata gctattaaat aggctatcac cctttcctac
     1261 cacatcaaga gtaatctggg ccatctgtcc caccttttcc tcaagagtca tcctatccag
     1321 catggcctta gccacctgtt cagcatcgct tcttttttca acgttgcaag agccaaaaat
     1381 caatgcaatt actgcaattc ccaatggcca caatcttatt ctatacatat tcttaagtgt
     1441 aggtaacaaa attcttttgt gctttataag tgtctttttg acaattttaa agtacaaata
     1501 taagcaggta attattagaa tcaaataaaa gtgtattttt aacacttatt tttcaaaaaa
     1561 aacaacttcg gctatttttt gaattaaatt tggcagataa actggtgggg ctttcgtaat
     1621 ttttaagcta agcttgcagc caaatatacg ttggtatgga tgatgagtta agactaatac
     1681 ccaacctttc gattgattgt gtaattttcg ggtgggatga gggtgtgctg aaggtgttgc
     1741 tccgaaagga gctgattgag tacaaggata ttgttcacat cgagtggaaa cttccaggga
     1801 accacgtggg gatgaacgag cggatagaag atacagccac gagggtactc aaggagcaag
     1861 ccggcgtgga gaacatcttt atgaagcagt tcatggtatt tagcgattta gaccgcttac
     1921 gccggcgcga aatggactac caatggatta aaaagtacgg acttggggag gat
//
LOCUS       DAOPHX010000217         1871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_88440_length_1871_cov_6.020374, whole genome shotgun sequence.
ACCESSION   DAOPHX010000217 DAOPHX010000000
VERSION     DAOPHX010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88440_length_1871_cov_6.020374"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..1661)
                     /gene="lpdA"
                     /locus_tag="PK502_10675"
     CDS             complement(273..1661)
                     /gene="lpdA"
                     /locus_tag="PK502_10675"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPH01322.1"
                     /translation="MNFDLIVIGSGPGGYVAAIRASQLGMKVAVVERENLGGICLNWG
                     CIPTKALLKSAQVYEYASHAADYGVKTTDVKPDFETMVARSRGVAETMSKGIQFLFKK
                     NEVTVINGFGKLKDNHTVSVTAPDGSTSDYTSSHIILATGARSRELPNLKQDGVRVIG
                     YRQALNMKRQPASMVVVGSGAIGSEFAYFYNAIGTKITLVEFLPNIVPVEDEEVSKAL
                     ERAFKKSGITVKTEATVESVDTSGELCRVSIQTKKGVEVVEAEVVLSAVGITPNIEGI
                     GLEELGISVEKGRVKVDEFYRTNVEGVYAIGDIIPGPALAHVASAEGITCVEKIAGLN
                     PEPINYNNIPGCTYTNPEISSVGMTEKAAVEAGYDVKVGKFPFTASGKATAAGQRDGF
                     VKLIFDARYGELLGAHLVGGNVTEMVAELVVARKLEITGHELIKSIHPHPTMSEAIME
                     AAAAAYGEAIHI"
BASE COUNT          431 a          485 c          443 g          512 t
ORIGIN      
        1 ttttttgtgc ggcaattgtg ggtattgggg ttgtttttaa ccctactttg gcttggtatg
       61 ctgcttggct gtaaaaaaaa aggtggtttg gcagggtcaa cctatatacc cggcgaaaga
      121 atatgctaca ggatggggga gggttaggaa atttaaagcc caagtgctta tggggcagat
      181 gccattattt gccataaaag cccatcccca acggttatgg gaatcgttgg ggatgagctg
      241 attaatcctg aagttgacta gcggtgttcc ggttatatgt ggatggcctc gccgtaggca
      301 gcagcagcgg cctccatgat ggcctcgctc atggtgggat gcgggtgtat gctcttgata
      361 agctcatgcc cggtgatttc gagtttccgg gcaacaacca gctccgctac catctcggta
      421 acgtttcccc caaccagatg ggcgccaagc agctcgccgt atctggcatc aaaaatcagc
      481 ttaacgaacc catcgcgttg tccggctgcg gttgccttgc ccgaggcggt aaatggaaac
      541 ttacctactt ttacatcata cccggcctcc actgcagcct tctcggtcat gcctaccgat
      601 gaaatctcgg ggttggtgta ggtgcagccg ggaatattat tgtagttaat gggctcgggg
      661 tttagaccgg caattttttc cacgcaggtt atcccctcag ccgatgccac atgggcaagc
      721 gcaggacctg gaattatatc cccaatggca tagacaccct caacgttggt gcggtagaac
      781 tcgtccacct ttacccgtcc cttctctacg gaaataccca gctcctcaag gcctatcccc
      841 tcaatgttag gggtaatgcc cacagccgaa agcaccacct ctgcctcaac cacctctact
      901 cccttttttg tctgaatgct taccctgcat agctcgccgg aggtatcaac cgactcaacg
      961 gtagcctctg ttttgacggt tatccccgat ttcttgaatg ctctttccag cgccttgctt
     1021 acctcctcgt cctcaaccgg aacaatgttt ggcaggaact caaccagggt aattttagtg
     1081 ccaatggcgt tgtagaagta ggcgaactcg ctgccaattg cgccggagcc gacaaccacc
     1141 atggaggcag gttgcctctt catgttcaat gcctgccggt agccaatcac cctaacgcca
     1201 tcctgcttaa ggttgggcag ctcgcgcgaa cgggctcctg ttgccagaat gatgtggctt
     1261 gacgtgtagt cggaggttga accatcggga gcggttacgc ttaccgtatg gttgtccttt
     1321 agcttcccaa acccgttgat tacggttacc tcatttttct taaaaagaaa ctgtattccc
     1381 ttgctcatgg tttcggcaac gcccctgctc cgcgccacca ttgtttcaaa atcgggtttt
     1441 acatcggttg tcttaacccc gtaatcggca gcatgtgagg catactcata aacctgcgca
     1501 cttttcagta gtgcctttgt tggaatgcaa ccccagttga ggcaaattcc tcccaggttt
     1561 tcacgctcca caacggcaac cttcatcccc agctggctgg cccgaatggc agcaacgtag
     1621 ccgcccggtc cgcttcctat tactatcagg tcgaaattca tatccggtaa tttttagttt
     1681 ttcaaaagta gtataacaaa cggtgatggg aaaatgttat tggacataac atgttaatcc
     1741 caaaccctcc aaaatcaggt aatatatttt caaaaaaagc ggaataatcg ggtaaatagt
     1801 ttgtttattc gatttttttg ttgtttctca gtattaccta aataagtaat attaaatggc
     1861 actttgtgta g
//
LOCUS       DAOPHX010000218         1557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_3318
            NODE_116142_length_1557_cov_5.366844, whole genome shotgun
            sequence.
ACCESSION   DAOPHX010000218 DAOPHX010000000
VERSION     DAOPHX010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865053
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:23:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,136
            CDSs (total)                      :: 2,102
            Genes (coding)                    :: 2,092
            CDSs (with protein)               :: 2,092
            Genes (RNA)                       :: 34
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 6 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116142_length_1557_cov_5.366844"
                     /isolate="AMR_MDS_3318"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /locus_tag="PK502_10680"
     CDS             complement(<1..1278)
                     /locus_tag="PK502_10680"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HPH01323.1"
                     /translation="MKGQKILIPIASAIIGGLVAVGISEGIYQNNRVNVATERDNNAT
                     VQLTRVFSGADSAYTDFTWAAEKTVDAVVHVTTSYTQTLDYSFGNPLFDFFFGPNGFD
                     QRRQVQSSGSGVILTNDGFIVTNNHVVENAEDITVVLNDKRKFSAKVVGADPNTDIAL
                     LKIDAKDLPTIPFGNSDDIKVGEWVLAIGNPFNLNSTVTAGIVSAKARNIQILSNREF
                     AIESFIQTDAAVNPGNSGGALVNLRGELIGINTAIASRTGTFAGYAFAVPSSIVKKVV
                     TDLIEFGEVRRAVMGVRIQELTDELAKEKGIKDIKGVYVADVEKNGAADKAGIKAGDV
                     ILSINNVDVNSTAQLQEQISRYRPDQQVDVLVNRDNTKKHYTVTLSNLKSEMKFSKSE
                     DTFSSLGATFAEVPAKLKRDLGIDYGLQVVELAD"
BASE COUNT          420 a          380 c          318 g          439 t
ORIGIN      
        1 atcggctagc tcaacaacct gaaggccata gtctatgccc aaatccctct tcagcttagc
       61 tggtacctcg gcaaaggttg caccaagact gctgaaggta tcttcagact ttgaaaactt
      121 catctcgctt ttcagattgc taagggtaac ggtgtaatgt ttcttagtat tatcacggtt
      181 taccagaaca tccacctgtt ggtcaggccg gtagcggcta atttgctcct gaagctgggc
      241 agtgctgttt acatcaacat tgttgattga aagaataaca tcgcccgctt tgatacctgc
      301 tttatcggca gcaccattct tttcaacatc ggcaacatac actcctttta tatccttaat
      361 gcctttttcc ttagccagct catcggttag ctcctggatt cgaacaccca taacagcacg
      421 ccgcacctca ccaaactcta ttaagtcggt aaccaccttt ttaactattg atgagggaac
      481 agcaaaggca taccctgcaa atgtgccagt acgggaagca atggcggtat taatgccaat
      541 gagctcaccc cttaggttaa ccagcgcacc accactattg cccgggttaa cagctgcgtc
      601 ggtttgaatg aacgattcaa tggcaaactc cctgttgcta agtatttgaa tgttacgagc
      661 tttagcactt acaatacctg cagtaaccgt tgagttaagg ttgaaagggt tgccaattgc
      721 caaaacccac tcccctacct taatgtcgtc ggaattacca aaaggaatgg tgggaaggtc
      781 cttggcatca attttaagaa gggcaatgtc ggtgttgggg tcggctccaa caacctttgc
      841 agaaaattta cgcttatcgt ttaaaactac ggtaatatcc tcggcatttt ccaccacatg
      901 attattggtt acgataaaac cgtcgtttgt aagaattaca cccgacccgg aactctgcac
      961 ttgacgtcgt tgatcgaatc cgtttggccc aaaaaagaag tcgaacaggg gattcccgaa
     1021 agagtagtcg agcgtttggg tgtacgatgt ggttacatgc accacagcat caaccgtttt
     1081 ttcggctgcc cacgtaaaat ctgtgtaggc tgaatcggct ccactaaaca cccgggtgag
     1141 ctgcactgta gcattattgt cccgctcagt ggcaacatta acccggttat tttgatagat
     1201 gccctccgaa atgcccactg ctaccaaacc accaataatg gctgaagcta taggtattaa
     1261 aattttttgt cctttcatag ctttaacgtt ttaagttctt gtaaaagtag caatcaaaaa
     1321 atatacccta agccgcccta accttttcct taacacttgt taacctcctg tatgtcagtt
     1381 tttcatattt ttatgaaaat tttctgacaa aatgaacagg gtaaaggata agataaactt
     1441 gtccgcctac cggcgtattc caaattcaaa aacctgtccg cctactggcg gaacttgtcc
     1501 ggctcctgac ggattcaccc ttcgacaggt gttacagtga gtagttcgta tggtgtt
//
